Adenosine Triphosphatases: A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.Magnesium: A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.Adenosine: A nucleoside that is composed of ADENINE and D-RIBOSE. Adenosine or adenosine derivatives play many important biological roles in addition to being components of DNA and RNA. Adenosine itself is a neurotransmitter.Acid Anhydride Hydrolases: A group of enzymes that catalyze the hydrolysis of diphosphate bonds in compounds such as nucleoside di- and tri-phosphates, and sulfonyl-containing anhydrides such as adenylylsulfate. (Enzyme Nomenclature, 1992) EC 3.6.Receptor, Adenosine A2A: A subclass of adenosine A2 receptors found in LEUKOCYTES, the SPLEEN, the THYMUS and a variety of other tissues. It is generally considered to be a receptor for ADENOSINE that couples to the GS, STIMULATORY G-PROTEIN.Nucleoside-Triphosphatase: An enzyme which catalyzes the hydrolysis of nucleoside triphosphates to nucleoside diphosphates. It may also catalyze the hydrolysis of nucleotide triphosphates, diphosphates, thiamine diphosphates and FAD. The nucleoside triphosphate phosphohydrolases I and II are subtypes of the enzyme which are found mostly in viruses.Receptor, Adenosine A1: A subtype of ADENOSINE RECEPTOR that is found expressed in a variety of tissues including the BRAIN and DORSAL HORN NEURONS. The receptor is generally considered to be coupled to the GI, INHIBITORY G-PROTEIN which causes down regulation of CYCLIC AMP.Adenosine Deaminase: An enzyme that catalyzes the hydrolysis of ADENOSINE to INOSINE with the elimination of AMMONIA.rac GTP-Binding Proteins: A sub-family of RHO GTP-BINDING PROTEINS that is involved in regulating the organization of cytoskeletal filaments. This enzyme was formerly listed as EC 3.6.1.47.Receptor, Adenosine A3: A subtype of ADENOSINE RECEPTOR that is found expressed in a variety of locations including the BRAIN and endocrine tissues. The receptor is generally considered to be coupled to the GI, INHIBITORY G-PROTEIN which causes down regulation of CYCLIC AMP.GTP Phosphohydrolases: Enzymes that hydrolyze GTP to GDP. EC 3.6.1.-.Receptor, Adenosine A2B: A subclass of adenosine A2 receptors found in the CECUM, the COLON, the BLADDER, and a variety of other tissues. It is generally considered to be a low affinity receptor for ADENOSINE that couples to the GS, STIMULATORY G-PROTEIN.Adenosine Kinase: An enzyme that catalyzes the formation of ADP plus AMP from adenosine plus ATP. It can serve as a salvage mechanism for returning adenosine to nucleic acids. EC 2.7.1.20.cdc42 GTP-Binding Protein: A member of the Rho family of MONOMERIC GTP-BINDING PROTEINS. It is associated with a diverse array of cellular functions including cytoskeletal changes, filopodia formation and transport through the GOLGI APPARATUS. This enzyme was formerly listed as EC 3.6.1.47.Receptors, Adenosine A2: A subclass of ADENOSINE RECEPTORS that are generally considered to be coupled to the GS, STIMULATORY G-PROTEIN which causes up regulation of CYCLIC AMP.Adenosine A2 Receptor Agonists: Compounds that selectively bind to and activate ADENOSINE A2 RECEPTORS.rho GTP-Binding Proteins: A large family of MONOMERIC GTP-BINDING PROTEINS that are involved in regulation of actin organization, gene expression and cell cycle progression. This enzyme was formerly listed as EC 3.6.1.47.Adenosine A2 Receptor Antagonists: Compounds that selectively bind to and block the activation of ADENOSINE A2 RECEPTORS.Receptors, Purinergic P1: A class of cell surface receptors that prefer ADENOSINE to other endogenous PURINES. Purinergic P1 receptors are widespread in the body including the cardiovascular, respiratory, immune, and nervous systems. There are at least two pharmacologically distinguishable types (A1 and A2, or Ri and Ra).Adenosine A1 Receptor Antagonists: Compounds that bind to and block the stimulation of ADENOSINE A1 RECEPTORS.rac1 GTP-Binding Protein: A rac GTP-binding protein involved in regulating actin filaments at the plasma membrane. It controls the development of filopodia and lamellipodia in cells and thereby influences cellular motility and adhesion. It is also involved in activation of NADPH OXIDASE. This enzyme was formerly listed as EC 3.6.1.47.Adenosine A1 Receptor Agonists: Compounds that bind to and stimulate ADENOSINE A1 RECEPTORS.Nucleotidyltransferases: A class of enzymes that transfers nucleotidyl residues. EC 2.7.7.Purinergic P1 Receptor Antagonists: Compounds that bind to and block the stimulation of PURINERGIC P1 RECEPTORS.Guanine Nucleotide Exchange Factors: Protein factors that promote the exchange of GTP for GDP bound to GTP-BINDING PROTEINS.Xanthines: Purine bases found in body tissues and fluids and in some plants.Purinergic P1 Receptor Agonists: Compounds that bind to and stimulate PURINERGIC P1 RECEPTORS.Adenosine Monophosphate: Adenine nucleotide containing one phosphate group esterified to the sugar moiety in the 2'-, 3'-, or 5'-position.

Membrane deinsertion of SecA underlying proton motive force-dependent stimulation of protein translocation. (1/12201)

The proton motive force (PMF) renders protein translocation across the Escherichia coli membrane highly efficient, although the underlying mechanism has not been clarified. The membrane insertion and deinsertion of SecA coupled to ATP binding and hydrolysis, respectively, are thought to drive the translocation. We report here that PMF significantly decreases the level of membrane-inserted SecA. The prlA4 mutation of SecY, which causes efficient protein translocation in the absence of PMF, was found to reduce the membrane-inserted SecA irrespective of the presence or absence of PMF. The PMF-dependent decrease in the membrane-inserted SecA caused an increase in the amount of SecA released into the extra-membrane milieu, indicating that PMF deinserts SecA from the membrane. The PMF-dependent deinsertion reduced the amount of SecA required for maximal translocation activity. Neither ATP hydrolysis nor exchange with external SecA was required for the PMF-dependent deinsertion of SecA. These results indicate that the SecA deinsertion is a limiting step of protein translocation and is accelerated by PMF, efficient protein translocation thereby being caused in the presence of PMF.  (+info)

Stable remodeling of tailless nucleosomes by the human SWI-SNF complex. (2/12201)

The histone N-terminal tails have been shown previously to be important for chromatin assembly, remodeling, and stability. We have tested the ability of human SWI-SNF (hSWI-SNF) to remodel nucleosomes whose tails have been cleaved through a limited trypsin digestion. We show that hSWI-SNF is able to remodel tailless mononucleosomes and nucleosomal arrays, although hSWI-SNF remodeling of tailless nucleosomes is less effective than remodeling of nucleosomes with tails. Analogous to previous observations with tailed nucleosomal templates, we show both (i) that hSWI-SNF-remodeled trypsinized mononucleosomes and arrays are stable for 30 min in the remodeled conformation after removal of ATP and (ii) that the remodeled tailless mononucleosome can be isolated on a nondenaturing acrylamide gel as a novel species. Thus, nucleosome remodeling by hSWI-SNF can occur via interactions with a tailless nucleosome core.  (+info)

Arginine methylation and binding of Hrp1p to the efficiency element for mRNA 3'-end formation. (3/12201)

Hrp1p is a heterogeneous ribonucleoprotein (hnRNP) from the yeast Saccharomyces cerevisiae that is involved in the cleavage and polyadenylation of the 3'-end of mRNAs and mRNA export. In addition, Hrplp is one of several RNA-binding proteins that are posttranslationally modified by methylation at arginine residues. By using functional recombinant Hrp1p, we have identified RNA sequences with specific high affinity binding sites. These sites correspond to the efficiency element for mRNA 3'-end formation, UAUAUA. To examine the effect of methylation on specific RNA binding, purified recombinant arginine methyltransferase (Hmt1p) was used to methylate Hrp1p. Methylated Hrp1p binds with the same affinity to UAUAUA-containing RNAs as unmethylated Hrpl p indicating that methylation does not affect specific RNA binding. However, RNA itself inhibits the methylation of Hrp1p and this inhibition is enhanced by RNAs that specifically bind Hrpl p. Taken together, these data support a model in which protein methylation occurs prior to protein-RNA binding in the nucleus.  (+info)

The Golgi apparatus plays a significant role in the maintenance of Ca2+ homeostasis in the vps33Delta vacuolar biogenesis mutant of Saccharomyces cerevisiae. (4/12201)

The vacuole is the major site of intracellular Ca2+ storage in yeast and functions to maintain cytosolic Ca2+ levels within a narrow physiological range. In this study, we examined how cellular Ca2+ homeostasis is maintained in a vps33Delta vacuolar biogenesis mutant. We found that growth of the vps33Delta strain was sensitive to high or low extracellular Ca2+. This strain could not properly regulate cytosolic Ca2+ levels and was able to retain only a small fraction of its total cellular Ca2+ in a nonexchangeable intracellular pool. Surprisingly, the vps33Delta strain contained more total cellular Ca2+ than the wild type strain. Because most cellular Ca2+ is normally found within the vacuole, this suggested that other intracellular compartments compensated for the reduced capacity to store Ca2+ within the vacuole of this strain. To test this hypothesis, we examined the contribution of the Golgi-localized Ca2+ ATPase Pmr1p in the maintenance of cellular Ca2+ homeostasis. We found that a vps33Delta/pmr1Delta strain was hypersensitive to high extracellular Ca2+. In addition, certain combinations of mutations effecting both vacuolar and Golgi Ca2+ transport resulted in synthetic lethality. These results indicate that the Golgi apparatus plays a significant role in maintaining Ca2+ homeostasis when vacuolar biogenesis is compromised.  (+info)

Deletion mutation analysis of the mutS gene in Escherichia coli. (5/12201)

The MutS protein is part of the dam-directed MutHLS mismatch repair pathway in Escherichia coli. We have constructed deletion derivatives in the mutS gene, which retain the P-loop coding region for ATP binding. The mutant proteins were assayed for ATP hydrolysis, heteroduplex DNA binding, heterodimer MutS formation, and the ability to interact with MutL. Dimerization was assayed by expressing His6-tagged wild-type and non-tagged deletion mutant proteins in the same cell and isolating the His6-tagged protein followed by MutS immunoblotting after SDS-polyacrylamide gel electrophoresis. MutS-MutL interaction was measured using the same technique except that the MutL protein carried the His6 tag. Our results indicate that DNA binding ability resides in the N-terminal end of MutS, and dimerization and MutL interactions are located in the C-terminal end. Given the extensive amino acid homology in the MutS family our results with E. coli should be applicable to MutS homologues in other prokaryotes and eukaryotes.  (+info)

Evolutionary dynamics of a mitochondrial rearrangement "hot spot" in the Hymenoptera. (6/12201)

The arrangement of tRNA genes at the junction of the cytochrome oxidase II and ATPase 8 genes was examined across a broad range of Hymenoptera. Seven distinct arrangements of tRNA genes were identified among a group of wasps that have diverged over the last 180 Myr (suborder Apocrita); many of the rearrangements represent evolutionarily independent events. Approximately equal proportions of local rearrangements, inversions, and translocations were observed, in contrast to vertebrate mitochondria, in which local rearrangements predominate. Surprisingly, homoplasy was evident among certain types of rearrangement; a reversal of the plesiomorphic gene order has arisen on three separate occasions in the Insecta, while the tRNA(H) gene has been translocated to this locus on two separate occasions. Phylogenetic analysis indicates that this gene translocation is real and is not an artifactual translocation resulting from the duplication of a resident tRNA gene followed by mutation of the anticodon. The nature of the intergenic sequences surrounding this region does not indicate that it should be especially prone to rearrangement; it does not generally have the tandem or inverted repeats that might facilitate this plasticity. Intriguingly, these findings are consistent with the view that during the evolution of the Hymenoptera, rearrangements increased at the same time that the rate of point mutations and compositional bias also increased. This association may direct investigations into mitochondrial genome plasticity in other invertebrate lineages.  (+info)

Characterisation of copper-binding to the second sub-domain of the Menkes protein ATPase (MNKr2). (7/12201)

The Menkes ATPase (MNK) has an essential role in the translocation of copper across cellular membranes. In a complementary manner, the intracellular concentration of copper regulates the activity and cellular location of the ATPase through its six homologous amino-terminal domains. The roles of the six amino-terminal domains in the activation and cellular trafficking processes are unknown. Understanding the role of these domains relies on the development of an understanding of their metal-binding properties and structural properties. The second conserved sub-domain of MNK was over-expressed, purified and its copper-binding properties characterised. Reconstitution studies demonstrate that copper binds to MNKr2 as Cu(I) with a stoichiometry of one copper per domain. This is the first direct evidence of copper-binding to the MNK amino-terminal repeats. Circular dichroism studies suggest that the binding or loss of copper to MNKr2 does not cause substantial changes to the secondary structure of the protein.  (+info)

The interaction of the human MutL homologues in hereditary nonpolyposis colon cancer. (8/12201)

Germline mutations in two human mismatch repair (MMR) genes, hMSH2 and hMLH1, appear to account for approximately 70% of the common cancer susceptibility syndrome hereditary nonpolyposis colorectal cancer (HNPCC). Although the hMLH1 protein has been found to copurify with another MMR protein hPMS2 as a heterodimer, their function in MMR is unknown. In this study, we have identified the physical interaction regions of both hMLH1 with hPMS2. We then examined the effects of hMLH1 missense alterations found in HNPCC kindreds for their interaction with hPMS2. Four of these missense alterations (L574P, K616Delta, R659P, and A681T) displayed >95% reduction in binding to hPMS2. Two additional missense alterations (K618A and K618T) displayed a >85% reduction in binding to hPMS2, whereas three missense alterations (S44F, V506A, and E578G) displayed 25-65% reduction in binding to hPMS2. Interestingly, two HNPCC missense alterations (Q542L and L582V) contained within the consensus interaction region displayed no effect on interaction with hPMS2, suggesting that they may affect other functions of hMLH1. These data confirm that functional deficiencies in the interaction of hMLH1 with hPMS2 are associated with HNPCC as well as suggest that other unknown functional alteration of the human MutL homologues may lead to tumorigenesis in HNPCC kindreds.  (+info)

*ATPase

... s (EC 3.6.1.3, adenylpyrophosphatase, ATP monophosphatase, triphosphatase, SV40 T-antigen, adenosine 5'-triphosphatase, ... Kielley, WW (1961). "Myosin adenosine triphosphatase". In Boyer, P. D.; Lardy, H.; Myrbäck, K. The Enzymes. 5 (2nd ed.). New ... Martin SS, Senior AE (November 1980). "Membrane adenosine triphosphatase activities in rat pancreas". Biochimica et Biophysica ... adenosine triphosphatase) are a class of enzymes that catalyze the decomposition of ATP into ADP and a free phosphate ion. This ...

*Machine perfusion

Membrane adenosine triphosphatase and cation transport. Br Med Bull 1968;24(2):165-169. Levy MN. Oxygen consumption and blood ...

*ATP synthase

8. Properties of a factor conferring oligomycin sensitivity on mitochondrial adenosine triphosphatase". The Journal of ... "Catalytic site cooperativity of beef heart mitochondrial F1 adenosine triphosphatase. Correlations of initial velocity, bound ... ATP synthase is an enzyme that creates the energy storage molecule adenosine triphosphate (ATP). ATP is the most commonly used ... It is formed from adenosine diphosphate (ADP) and inorganic phosphate (Pi). The overall reaction catalyzed by ATP synthase is: ...

*Propionigenium modestum

Mitchell, Peter D. "A chemiosmotic molecular mechanism for proton-translocating adenosine triphosphatases". 43 (2). Amsterdam: ... Laubinger, Werner; Dimroth, Peter (1989). "The sodium ion translocating adenosine triphosphatase of Propionigenium modestum ...

*Discovery and development of proton pump inhibitors

"Gastric adenosine triphosphatases: A review of their possible role in HCl secretion". Gastroenterology. 73 (4 Pt 2): 921-6. ...

*Đái Duy Ban

Dai Duy Ban: (Announcement in Japan in English): Adenosine Triphospha tases on Plasma. Membrane Surrounding Lipid Vacuoles in L ...

*Oxidative phosphorylation

I. Purification and properties of soluble dinitrophenol-stimulated adenosine triphosphatase". J. Biol. Chem. 235 (11): 3322-9. ... The ATP synthase uses the energy to transform adenosine diphosphate (ADP) into adenosine triphosphate, in a phosphorylation ... "Catalytic site cooperativity of beef heart mitochondrial F1 adenosine triphosphatase. Correlations of initial velocity, bound ... thereby releasing energy which is used to produce adenosine triphosphate (ATP). In most eukaryotes, this takes place inside ...

*1957 in science

February 1957). "The Influence of Some Cations on an Adenosine Triphosphatase from Peripheral Nerves". Biochimica et Biophysica ...

*Na /K -ATPase

Na+ /K+ -ATPase (sodium-potassium adenosine triphosphatase, also known as the Na+ /K+ pump or sodium-potassium pump) is an ... ISBN 978-0-8493-3978-3. Skou JC (February 1957). "The influence of some cations on an adenosine triphosphatase from peripheral ... For every adenosine triphosphate molecule that the pump use, three sodium ions are exported, while two potassium are imported; ... The sodium pump is a membrane protein that use energy in the form of adenosine triphosphate to perform active transport of ...

*ATP5E

8. Properties of a factor conferring oligomycin sensitivity on mitochondrial adenosine triphosphatase". The Journal of ...

*P-type ATPase

doi:10.1016/0968-0004(87)90071-5. SKOU JC (February 1957). "The influence of some cations on an adenosine triphosphatase from ... adenosine triphosphate (ATP). In addition, they all appear to interconvert between at least two different conformations, ...

*Action potential

Skou J (1957). "The influence of some cations on an adenosine triphosphatase from peripheral nerves". Biochim Biophys Acta. 23 ...

*Jens Christian Skou

Skou, J C (1989). "The influence of some cations on an adenosine triphosphatase from peripheral nerves. 1957". Biochim. Biophys ... The Influence of Some Cations on an Adenosine Triphosphatase from Peripheral Nerves'". Biochim. Biophys. Acta. 1000: 435-8. doi ...

*MT-ATP6

8. Properties of a factor conferring oligomycin sensitivity on mitochondrial adenosine triphosphatase". The Journal of ... Another segment of the enzyme uses the energy created by this proton flow to convert a molecule called adenosine diphosphate ( ...

*ATP synthase, H+ transporting, mitochondrial F1 complex, alpha 1

8. Properties of a factor conferring oligomycin sensitivity on mitochondrial adenosine triphosphatase". The Journal of ...

*Colworth Medal

The adenosine triphosphatase reactions of myosin and actomyosin and their relation to energy transduction in muscle". ...

*Meiotic recombination checkpoint

An AAA-adenosine triphosphatase (AAA-ATPase) was found to be essential in this pathway. but the mechanism is not yet clear. ...

*Secretin

... adenosine triphosphatase". Gastroenterology. 108 (3): 850-9. doi:10.1016/0016-5085(95)90460-3. PMID 7875488. Marinelli RA, Pham ...

*Homing endonuclease

... adenosine triphosphatase". Science. 250 (4981): 651-7. doi:10.1126/science.2146742. PMID 2146742. Perler FB, Comb DG, Jack WE, ... translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae". J Biol Chem. 265 (12): 6726-33. ...

*ATP6AP2

This protein is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in ...

*Prostasomes

Ronquist, G; Brody, I; Gottfries, A; Stegmayr, B (1978). [PM:152589 "An Mg2+ and Ca2+-stimulated adenosine triphosphatase in ... Ronquist, G; Brody, I; Gottfries, A; Stegmayr, B (1978). [PM:153718 "An Mg2+ and Ca2+-stimulated adenosine triphosphatase in ... Ronquist, G; Hedström, M (1977). [PM:142513 "Restoration of detergent-inactivated adenosine triphosphatase activity of human ... "Abnormal deficiency of both Mg2+ and Ca2+-dependent adenosine triphosphatase and secretory granules and vesicles in human ...

*Histone H2A

... related adenosine triphosphatase. Another H2A variant that has been identified is H2AX. This variant has a C-terminal extension ...

*Protein splicing

... translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae". J. Biol. Chem. 265 (12): 6726-33. ... adenosine triphosphatase". Science. 250 (4981): 651-7. doi:10.1126/science.2146742. PMID 2146742. Short review Starokadomskyy ... The protein splicing reactions which are known now do not require exogenous cofactors or energy sources such as adenosine ...

*Serotonin transporter

... properly the Serotonin Transporter requires the membrane potential created by the sodium-potassium adenosine triphosphatase. ...

*V-ATPase

February 2002). "Novel mutations in the a3 subunit of vacuolar H+ -adenosine triphosphatase in a Japanese patient with ...

*List of homing endonuclease cutting sites

... adenosine triphosphatase". Science. 250 (4981): 651-7. doi:10.1126/science.2146742. PMID 2146742. Gimble FS, Thorner J (May ... translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae". J Biol Chem. 265 (12): 6726-33. ...
MARTA ISERN DE CALDENTEY, KENNETH P. WHEELER; Interactions of Phospholipids with Sodium-plus-Potassium Ion-Dependent Adenosine Triphosphatase. Biochem Soc Trans 1 February 1977; 5 (1): 107-108. doi: https://doi.org/10.1042/bst0050107. Download citation file:. ...
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BACKGROUND: Neurons can survive for decades via cell maintenance and protein degradation. This process includes the general protein endolysosomal degradation pathway, an integral part of which is the Rab GTPase proteins ...
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Levental, M and Tabakoff, B, "Sodium-potassium-activated adenosine triphosphatase activity as a measure of neuronal membrane characteristics in ethanol-tolerant mice." (1980). Subject Strain Bibliography 1980. 2608 ...
Kasarov, L B. and Friedman, H, "Enhanced na+-k+-activated adenosine triphosphatase activity in trans- formed fibroblasts." (1974). Subject Strain Bibliography 1974. 1678 ...
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Sigma-Aldrich offers abstracts and full-text articles by [Chuan-Xi Mao, Ying Xiong, Zhaohuan Xiong, Qifu Wang, Yong Q Zhang, Shan Jin].
integral component of plasma membrane, intracellular membrane-bounded organelle, calcium-transporting ATPase activity, cellular calcium ion homeostasis
BioAssay record AID 299465 submitted by ChEMBL: Inhibition of MKLP1 assessed as inhibition ATP hydrolysis by ATPase assay at 20 uM.
The aim of the present study was to evaluate the antioxidant effect of a new steroid (4-chloro-17α-acetoxy-4-pregnene-3,20-dione) in rat brain. Twelve adults rats of 500g were randomly distributed in groups of 6 that were treated with 4-chloro-17α-acetoxy-4-pregnene-3,20-dione (4 mg/kg/day), or vehicle, administered during 5 days. At the end of treatment all animals were sacrificed and the GSH levels in the brain were measured by fluorescence method, as well as lipid peroxidation (TBARS), Na+,K+-ATPase and total ATPase enzymatic activity by spectrophotometry methods. Na+,K+-ATPase and total ATPase activities increased, only the total ATPase activity increase was significant as compared to the control group ( ...
VPS4A is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In…
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
ATAD3A antibody (ATPase family, AAA domain containing 3A) for IHC-P, WB. Anti-ATAD3A pAb (GTX116301) is tested in Human samples. 100% Ab-Assurance.
Roles of eif3e and eif3min protein synthesis. (A) Subcellular localization. Live cells expressing GFP-tagged alleles of eif3b, eif3e, and eif3m at the endogenou
View mouse Ubxn10 Chr4:138709837-138737167 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
Sodium:potassium-exchanging ATPases are tetrameric proteins, consisting of two large alpha subunits and two smaller beta subunits. The alpha subunits bear the active site and penetrate the membrane, while the beta subunits carry oligosaccharide groups and…
Testing nucleotide-protein interaction - posted in Biochemistry: Hi everybody I work with an intrinsically disordered protein with unknown function and no close homologs. Psi Blasts show some low degree of homology to ATPsaes, but with e-values above cutoff. Motif searches predict an incomplete Walker A motif. If so, only the GKS motif is conserved, but not the P-loop. The protein forms a stable complex with Ca-calmodulin. I tried atpase assays, but there was no activity. Upon addition...
MLFDLINSFL KNGINNSNNN NNNNNNKNNF YNSLEDDDYL LNNQTTKVSL YLYFFIFAFM FLVVDLIMLY YKHRENIESR ETDLSLKLNK MLIDFENDNK IKSSPTTSTT TTTITPTTTS SSQLRQPSTP KTTTKTINSP PSTPKSPPPL PSLESKLLYK DDIKQQLSLN EAKSQIDSAK QLDESLKYNS CIKLYIDGIE KLMALFSSYN SKEYRDYIDF YLKRAEYLKN ELKKGTNLKS ITNFNNFSKE YQINYNNKIL EQQQQQQQQS SSTYRNSLNL SSSKSNSTIN NRHSISSLSS LNSTTATTTT PSNTSTITSP GNKYGLQKSL SSTTLSLKKS SNSTNFQQPS PPSMVIPDIK GIDKSMVTLI MNEIMDRKNP VKWDDVVGLD KVKQSLMESV ILPNLRPDVF TGLRAPPKGL LLFGPPGNGK TMIAKAVAYE SKVTFFSISS SSLTSKYVGD GEKLVRALFA VATHFQPSII FIDEIDSLLT ERSSNESEAS RRLKTEILVQ FDGARTNGDE RVLVMGATNR PEDLDDAALR RLVKRIYVGL PELETRLQII QHLLVGQRHS LTKQQINSLA EVTQGYSGFD LAALCKDAAY EPIRRLGIGI KDLELNEISL ISFKDFANSL KQIRPSVTSQ SLKSFEKWNQ KFGTI ...
We have used all reasonable efforts to ensure that the information displayed on these pages and contained in the databases is of high quality. We make no warranty, express or implied, as to its accuracy or that the information is fit for a particular purpose, and will not be held responsible for any consequences arising out of any inaccuracies or omissions. Individuals, organisations and companies which use this database do so on the understanding that no liability whatsoever either direct or indirect shall rest upon the curator(s) or any of their employees or agents for the effects of any product, process or method that may be produced or adopted by any part, notwithstanding that the formulation of such product, process or method may be based upon information here provided ...
The effect of uranyl-nitrate (10102064) (UN) on adenosine- triphosphatase (9000833) (ATPase) was studied in dog, cat, hamster, rat, rabbit and human tissue. Brain, heart, kidney, and liver homogenates or microsomal fractions were incubated with UN at 37 degrees C. Fifty percent microsomal ATPase inhibition (I50) required about 0.045 millimolar (mM) UN for dog heart, brain and kidney, while 0.08mM
SecA is a central component of the general secretion system that is essential for bacterial growth and thus an ideal target for antimicrobial agents. A series of fluorescein analogues were first screened against the ATPase activity using the truncated unregulated SecA catalytic domain. Rose bengal (RB) and erythrosin B (EB) were found to be potent inhibitors SecA with IC50 values of 0.5 μM and 2 μM, respectively. RB and EB inhibit the catalytic SecA ATPase more effectively than the F1F0-proton ATPase. We used three assays to test the effect of these compounds on full-length SecA ATPase: in solution (intrinsic ATPase), in membrane preparation, and translocation ATPase. RB and EB show the following trend in terms of IC50 values: translocation ATPase,membrane ATPase,intrinsic ATPase. Very importantly, the potency of these fluorescein analogues in inhibiting the truncated SecA ATPase correlates with their ability to inhibit the biologically relevant protein translocation activity of SecA. The in ...
A cell surface-located nucleoside triphosphatase activity can be assayed in liver epithelial cultures in situ with the incubation of intact cells in a medium containing [γ-32P]adenosine triphosphate and correlated with the tumorigenicity of these cells in neonatal Wistar rats. The ectoenzyme activity of normal diploid cell lines is minimal, whereas a considerably high activity has been found in all tumorigenic cell lines tested. The optimum condition for the adenosinetriphosphatase activity is physiological with regard to osmolarity, ionic composition, pH, and substrate concentration in the medium. The enzyme is significantly stimulated by Ca2+, and its activation is controlled by Mg2+. Histochemical examinations indicate that glutaraldehyde-fixed cells of tumorigenic lines have Ca2+-stimulated adenosinetriphosphatase activity on the external surface. The isotopic assay of adenosine triphosphate hydrolysis by intact cells may provide a rapid method for screening oncogenesis in vitro of liver ...
LOCALIZATION OF (SODIUM ION, POTASSIUM ION)-ACTIVATED ADENOSINE TRIPHOSPHATASE ACTIVITY IN TELEOST CHLORIDE CELLS: CYTOCHEMICAL AND CELLISOLATION STUDIES ON THE BRANCHIAL EPITHELIUM OF THE PINFISH, LAGODON ...
Synonyms for adenosine triphosphatase test in Free Thesaurus. Antonyms for adenosine triphosphatase test. 2 words related to adenosine: biochemistry, nucleoside. What are synonyms for adenosine triphosphatase test?
Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3 to 5 direction.
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Abstract: Within a daily period maximal activity of ATPases and content of 32P in Pliss lymphosarcoma tissue was found in the samples taken at 15 or 21 p.m. and 3 a.m. The radioactivity in the tumor was minimal between 9 and 12 a.m. and 18 or 24 p.m. The alterations in Mg2+-ATPase activity in their magnitude and circadian rhythm coincided completely with the daily variations in content of radiophosphorus. Activities of Na+, K+- and HCO(3-)-ATPases exhibited maximal values at 15 p.m. and, especially between 3 and 6 a.m., i.e. these alterations were of two-peak shape without the peak at 21 p.m ...
Spastin and katanin are ring-shaped hexameric AAA ATPases that sever microtubules, and thus crucially depend on a physical interaction with microtubules. For the first time, we report here the microtubule binding properties of spastin at the single-molecule level, and compare them to katanin. Microscopic fluorescence assays showed that human spastin bound to microtubules by ionic interactions, and diffused along microtubules with a diffusion coefficient comparable to katanin. The microscopic measurement of landing and dissociation rates demonstrated the ionic character of the interaction, which could be mapped to a patch of three lysine residues outside of the catalytic domain of human spastin. This motif is not conserved in Drosophila spastin or katanin, which also bound by non-catalytic parts of the protein. The binding affinities of spastin and katanin were nucleotide-sensitive, with the lowest affinities under ADP,, the highest under ATP-γS conditions. These changes correlated with the formation of
The effect of isoproterenol on the electrophysiological properties of the S2 proximal segment of the rabbit was examined. Isoproterenol at 10(-8) to 10(-4) M depolarized the basolateral membrane voltage (Vb) in a dose-dependent manner. Propranolol attenuated the isoproterenol-induced depolarization. These possible mechanisms of cell depolarization were explored. The role of luminal Na(+)-organic solute cotransport was negligible, since the removal of organic solute did not change the depolarization. Basolateral Na(+)-(HCO3-) cotransport was supported by the finding that 4,4-diisothiocyanostilbene-2,2-disulfonic acid inhibited isoproterenol-induced depolarization. Basolateral K+ conductance was suggested by the finding that the application of Ba2+ blocked the isoproterenol-induced depolarization. Na(+)-K(+)-adenosine-triphosphatase (ATPase) was questionable. Although ouabain blocked isoproterenol-induced depolarization, the removal of Na+ did not inhibit the depolarization. Further experiment ...
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Hsp90 couples ATP hydrolysis to large conformational changes essential for activation of client proteins. The structural transitions involve dimerization of the N-terminal domains and formation of closed states involving the N-terminal and middle domains. Here, we used Hsp90 mutants that modulate ATPase activity and biological function as probes to address the importance of conformational cycling for Hsp90 activity. We found no correlation between the speed of ATP turnover and the in vivo activity of Hsp90: some mutants with almost normal ATPase activity were lethal, and some mutants with lower or undetectable ATPase activity were viable. Our analysis showed that it is crucial for Hsp90 to attain and spend time in certain conformational states: a certain dwell time in open states is required for optimal processing of client proteins, whereas a prolonged population of closed states has negative effects. Thus, the timing of conformational transitions is crucial for Hsp90 function and not cycle ...
This work establishes Lis1 as a dynein regulatory protein and links its function to dynein enzymatic activity. The simplest interpretation of our data is that Lis1 acts specifically and directly on dynein. Although the increase in ATPase activity is modest at first glance, our determination that only one-third of the dynein molecules interacted with Lis1 suggests that there were two populations of motors present in the ATPase assay. The major population did not stably bind Lis1 and is not expected to have had any change in ATPase activity, whereas a smaller population binds Lis1, resulting in a boost in ATPase activity. In this scenario, the enzymatic activity of the dynein as a whole would have increased by only 40%, but the activity of Lis1-associated motors may have increased by ∼100%.. The finding that Lis1 can stimulate dynein in vitro supports our model that Lis1 has an activating influence on dynein in cells (Smith et al., 2000). In our previous work, we found that raising Lis1 ...
This work establishes Lis1 as a dynein regulatory protein and links its function to dynein enzymatic activity. The simplest interpretation of our data is that Lis1 acts specifically and directly on dynein. Although the increase in ATPase activity is modest at first glance, our determination that only one-third of the dynein molecules interacted with Lis1 suggests that there were two populations of motors present in the ATPase assay. The major population did not stably bind Lis1 and is not expected to have had any change in ATPase activity, whereas a smaller population binds Lis1, resulting in a boost in ATPase activity. In this scenario, the enzymatic activity of the dynein as a whole would have increased by only 40%, but the activity of Lis1-associated motors may have increased by ∼100%.. The finding that Lis1 can stimulate dynein in vitro supports our model that Lis1 has an activating influence on dynein in cells (Smith et al., 2000). In our previous work, we found that raising Lis1 ...
Adenosine triphosphate (ATP) is an important macro molecule that is the prime supplier of energy to run cell metabolism and to regulate several physiological processes in a cell. Despite its central role, the number of non-invasive methods to study ATP on a single cell level in real time are limited. ATPases use energy derived from ATP hydrolysis to maintain cell membrane potential by regulating ion gradients across the plasma membrane. It is generally believed that the Na+/K+-ATPase (NKA) which belongs to the P-type ATPase superfamily represent the main energy consumer among the ATPases. In this study, we set out to quantify ATP consumption by NKA on a single cell level in human embryonic kidney cells (HEK293a) using PercevalHR, a genetically encoded fluorescent biosensor that reports changes in the ATP:ADP during live cell imaging. We demonstrate that ATP hydrolysis by NKA is faster at physiological temperatures (35 -37°C) compared to room temperature. K+ free KREBS pre-treatment increased ...
The microtubule (MT)‐severing enzyme katanin triggers dynamic reorientation of cortical MT arrays that play crucial functions during plant cell morphogenesis, such as cell elongation, cell wall biosynthesis, and hormonal signaling. MT severing specifically occurs at crossover or branching nucleation sites in living Arabidopsis cells. This differs from the random severing observed along the entire length of single MTs in vitro and strongly suggests that a precise control mechanism must exist in vivo. However, how katanin senses and cleaves at MT crossover and branching nucleation sites in vivo has remained unknown. Here, we show that the katanin p80 subunit KTN80 confers precision to MT severing by specific targeting of the katanin p60 subunit KTN1 to MT cleavage sites and that KTN1 is required for oligomerization of functional KTN80-KTN1 complexes that catalyze severing. Moreover, our findings suggest that the katanin complex in Arabidopsis is composed of a hexamer of KTN1-KTN80 heterodimers ...
Implementing protein degradation is integral to enabling the function of many dynamical circuits, such as oscillators or feed-forward loops. We have been exploring the overexpression of AAA ATPases ClpXP to selectively target proteins with degradation tags. Initial experiments indicate that ClpXP, when overexpressed, can accelerate degradation of purified eGFP-ssrA (Fig. 1). We will further characterize this AAA ATPase family, as well as try to demonstrate its use through an incoherent feed-forward loop. We have not been able to replicate the results by adding purified ClpXP protein. However, we intend to express ClpXP off of plasmids or to create custom extract with ClpXP already overexpressed to enable rapid protein degradation. ...
Subunit Of The 19S Regulatory Particle Of The 26S Proteasome Lid; Synthetically Lethal With RPT1, Which Is An ATPase Component Of The 19S Regulatory Particle; Physically Interacts With Nob1p And Rpn3p; Protein Abundance Increases In Response To DNA Replication Stress
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 3 subunit. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012 ...
1GAJ: Crystal structures of the MJ1267 ATP binding cassette reveal an induced-fit effect at the ATPase active site of an ABC transporter.
1gaj: Crystal structures of the MJ1267 ATP binding cassette reveal an induced-fit effect at the ATPase active site of an ABC transporter.
Histoenzymological techniques were used to examine ATPase activity in rat heart muscle fibres after experimental infarction. 25 hours after coronary ligation, ATPase activity in all ventricular section fibres was high, homogeneous at pH 9.4, sections
View mouse Ncapg2 Chr12:116405355-116463532 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
The long-range goal of this project is to determine the mechanism used by bacteria to select the proper cell division site at midcell. The three Min proteins, M...
TbVCP Trypanosoma (Janet Roggy and Jay Bangs, Ph.D., University of Wisconsin-Madison, Department of Medical Microbiology and Immunology, 1300 University Avenue, Madison, WI 53711, USA; January 22, 1998, GenBank database entry from February 9, 1998 ...
The erythrocyte ATPase activities taken from normal rats when incubated in supernates of boiled plasma from control normotensive and experimental hypertensive r
Monoclonal antibody against ATPase family AAA domaining containg 2 expressed by ATAD2 for use in Immunoprecipitation, Microarray, Western Blot against Human
Since these processes use ATP, they all involve ATPase enzymes. ATPases catalyse the hydrolysis of ATP to ADP + Pi, and do work with the energy released.. All the thousands of chemical reactions taking place in a cell are referred to as Metabolism.. To make the reactions easier to understand, biochemists arrange them into metabolic pathways.The intermediates in these metabolic pathways are called metabolites.. ...
TadABC complex; the Flp pilus assembly complex, consisting of one ATPase component, TadA or CpaF, and two membrane components, TadB (218 aas and 3 - 5 TMSs) and TadC (200 aas and 2 N- and C-terminal TMSs) (Bottacini et al. 2017 ...
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Part Of Evolutionarily-conserved CCR4-NOT Regulatory Complex; Contains Single ABC-type ATPase Domain But No Transmembrane Domain; Interacts With Several Subunits Of Mediator
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Mutations in Spastin are associated with Hereditary Spastic Paraplegia (HSP), a disease characterized by retrograde axonal degeneration and a spastic gait. On page 599, Evans et al. demonstrate that Spastin is a microtubule-severing protein. The result leaves an unresolved paradox: how can the loss of a microtubule-severing protein result in shorter microtubule fibers?. Spastin is a member of the AAA ATPase protein family, and thus has a highly conserved ATP-binding domain. Within the family, Spastin is most like Katanin, a microtubule-severing protein required for the function of worm meiosis and rodent neurons. Spastin overexpression decreases the number of microtubules in cells, suggesting that it too might cut microtubules.. The team generated point mutations that disrupted ATP binding or hydrolysis. ATP binding was required for Spastin association with microtubules, ATP hydrolysis was necessary for microtubule release, and addition of recombinant Spastin severed microtubules in ...
Doss-Pepe, E.W., et al. (2003). "Ataxin-3 interactions with Rad23 and valosin-containing protein and its associations with ubiquitin chains and the proteasome are consistent with a role in ubiquitin-mediated proteolysis." Mol. Cell. Biol 23(18): 6469-6483. ...
Doss-Pepe, E.W., et al. (2003). "Ataxin-3 interactions with Rad23 and valosin-containing protein and its associations with ubiquitin chains and the proteasome are consistent with a role in ubiquitin-mediated proteolysis." Mol. Cell. Biol 23(18): 6469-6483. ...
Eps15 homology domain (EHD) proteins are conserved adenosine triphosphatases that are involved in membrane remodeling. EHD family members are structurally similar to the guanosine triphosphatase (GTPase) dynamin, and both are essential for the fission step of clathrin-mediated endocytosis. This Journal Club highlights a recent study by Jakobsson et al. that reports the unexpected finding that, rather than having a redundant function, EHD can regulate dynamin activity. Dynamin helices assemble around the neck of budding endocytic vesicles; as dynamin helices lengthen, the neck of the growing bud may become so long that GTP hydrolysis is no longer sufficient to promote fission. EHD increases the efficiency of dynamin-induced fission by restricting the length of dynamin helices. Furthermore, EHD is able to bind both dynamin and amphiphysin. Therefore, we propose a model whereby amphiphysin recruits both EHD and dynamin in neurons to regulate clathrin-dependent synaptic vesicle endocytosis.. ...
The VCP gene provides instructions for making an enzyme called valosin-containing protein. This enzyme is found throughout the body and has a wide variety of functions within cells. It is involved in cell division, fusing membranes within cells, reassembling cell structures after cells have divided, preventing the self-destruction of cells (apoptosis), and repairing damaged DNA.. Valosin-containing protein is part of the ubiquitin-proteasome system, which is the machinery that breaks down (degrades) unneeded proteins within cells. This system provides quality control by disposing of damaged, misshapen, and excess proteins. It also regulates the level of proteins involved in several critical cell activities, such as the timing of cell division and growth. Researchers believe that most of the functions of valosin-containing protein are directly or indirectly related to the ubiquitin-proteasome system. ...
Symptoms of Adenosine triphosphatase deficiency, anaemia due to including 12 medical symptoms and signs of Adenosine triphosphatase deficiency, anaemia due to, alternative diagnoses, misdiagnosis, and correct diagnosis for Adenosine triphosphatase deficiency, anaemia due to signs or Adenosine triphosphatase deficiency, anaemia due to symptoms.
Previous work has shown that spastin, the SPAST gene product, shares homology with the p60 subunit of katanin, and both proteins have been shown to be ATP-dependent microtubule-severing proteins. Microtubules, components of the cells cytoskeleton, are fundamentally important for the correct outgrowth of axons, but how microtubule-severing activities might relate to axonal degeneration in HSP is unclear. This study uses confocal time-lapse imaging in the zebrafish embryonic CNS to demonstrate that both spastin and katanin are required in axons for the formation of forward-moving dynamic microtubules, and also at the growth cone, the specialised guidance structure at the axons growing tip. The effects of lowering the levels of spastin and katanin, mimicking haploinsufficiency, were monitored in embryonic neurons using a fluorescent protein as a marker for the growing microtubules. Reduced expression of either spastin or katanin severely impaired formation of dynamic microtubules and inhibited ...
In molecular biology, SWI/SNF (SWItch/Sucrose Non-Fermentable), is a nucleosome remodeling complex found in both eukaryotes and prokaryotes. In simpler terms, it is a group of proteins that associate to remodel the way DNA is packaged. It is composed of several proteins - products of the SWI and SNF genes (SWI1, SWI2/SNF2, SWI3, SWI5, SWI6) as well as other polypeptides. It possesses a DNA-stimulated ATPase activity and can destabilise histone-DNA interactions in reconstituted nucleosomes in an ATP-dependent manner, though the exact nature of this structural change is unknown. The human analogs of SWI/SNF are BAF (SWI/SNF-A) and PBAF (SWI/SNF-B). BAF in turn stands for "BRG1- or HBRM-associated factors", and PBAF is for "polybromo-associated BAF". It has been found that the SWI/SNF complex (in yeast) is capable of altering the position of nucleosomes along DNA. Two mechanisms for nucleosome remodeling by SWI/SNF have been proposed. The first model contends that a unidirectional diffusion of a ...
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The peroxisomal ABC-transporters Pxa1p and Pxa2p are half transporters. Previous genetic investigations have demonstrated that Pxa1p and Pxa2p have to dimerise in order to build a functional transporter, which is very likely involved in the import of long chain fatty acids into peroxisomes of S. cerevisiae. In this work, tagged versions of the proteins were purified as a complex. This proved for the building of a stable hetero dimer. For characterisation of the ATP binding properties, the transporters were incubated and cross linked with 8-azido-[alpha-32P]-ATP. This revealed an asymmetric binding of the ATP analogue. Pxa2p binds much more azido-ATP, than Pxa1p, while the dissociation constants are rather similar. The poorer ATP binding of Pxa1p is reflected by degenerated sequence motifs in the nucleotide binding fold. The purified ABC-transporters have been used for ATPase assays. They showed a basal ATPase activity, which could be stimulated by addition of long chain fatty acid CoAs, like ...
Condensins are pentameric complexes that were initially described as being involved in the dynamics of chromosomes during mitosis. It has been recently established that two related complexes (Condensin I and Condensin II) contribute to this process. An increasing sum of studies, using different approaches in various organisms, leads to the paradigm that Condensins are required for the correct segregation of replicated chromosomes by cooperating somehow with Topoisomerase II in sister chromatid resolution. Depending on species and/or experimental studies, these complexes also contribute to some aspects of the assembly and compaction of mitotic chromosomes. Recent studies provided evidences that Condensins and related complexes also function in non-mitotic processes such as replication and transcription. Biochemical studies have highlighted mechanistic aspects of Condensin function and initiated a fine functional dissection of core and regulatory subunits. However, the exact contribution of each subunit
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Although lithium ion substitutes poorly for potassium ion in the reaction cycle of (Na+ + K+)-ATPase (ATP phosphohydrolase, EC 3.6.1.3), it consistently activated this enzyme in the presence of sodium and potassium. In contrast, rubidium ion, a much more effective substitute for potassium than lithium, was generally inhibitory in the presence of sodium and potassium. Li+ did not appear to activate the enzyme by stimulating its phosphorylation from ATP. Rather, Na+ was required for Li+ to stimulate ATPase activity, and Li+ directly (though weakly) stimulated dephosphorylation. Under conditions such that increasing concentrations of K+ and Rb+ inhibited turnover of the enzyme, Li+ stimulated its activity above values observed with K+ or Rb+. It appears that Li+ stimulates the turnover of the ATPase by triggering its dephosphorylation and dissociating rapidly from the dephospho-enzyme, thus allowing the enzyme to rephosphorylate readily. With Rb+ the stability of the ...
The major action sites of alcohol are brain and liver, though it is not still clarified how the changes affect on the formation of tolerance and dependence. Recently proposed, alcohol primarily acts on the synaptic membranes, causing widespread changes in neurotransmission via effects on the numerous biochemical processes. That is to say, alcohol inhibits action potentials and active transports of cations, in which ATPase plays an important role as a carrier. The activities of membranebound enzymes such as Mg^++ ATPase and (Na^+ - K^+ )ATPase are influenced by alcohol. The author investigates on the changes of ATPase activities when ethanol is administered acutely, chronically up to 3 weeks, and withdrawan 6 hours after the last dose of ethanol up to 72 hours, to account with the effect of ethanol on sodium pump and cation transport. The results obtained are as follows: 1. Upon acute ethanol(30% w/v) administration, brain homogenates showed more significantly increased Mg^++ ATPase activities ...
Kushner, S. R., Fischer, L. M., & Hamilton, C. (1986). Dna Helicase-Ii(Uvrd) and the Rep Helicase Are Required for Escherichia-Coli Dna-Replication. Journal of Cellular Biochemistry, 178-178 ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
An enzyme that catalyzes the Hydrolysis of ATP and is activated by millimolar concentrations of either Ca(2+) or Mg(2+). Unlike Ca(2+)-Transporting ATPase it does not require the second divalent cation for its activity, and is not sensitive to orthovanadate. (Prog Biophys Mol Biol 1988;52(1):1). A subgroup of EC 3.6.1.3 ...
A - Tilt: 4° - Segments: 1( 68- 84), 2( 89- 112), 3( 242- 265), 4( 272- 291), 5( 645- 666), 6( 673- 690), 7( 709- 731), 8( 752- 772), 9( 785- 803), 10( 817- 841 ...
Expression and characterization of P-type ATPases for structural studies [Elektronische Ressource] / von Sivaram Chandra Chintalapati : Expression and Characterization ofP-type ATPases for Structural StudiesDissertationZur Erlangung des Doktorgrades der Naturwissenschaftenvorgelegt beim Fachbereich Biochemie, Chemie und PharmaziederJohann Wolfgang Goethe Universität in Frankfurt am MainvonSivaram Chandra Chintalapatiaus Tenali, IndienFrankfurt am Main 2007Die Arbeit wurde in der Abteilung Structural Biology des
We propose to further research the protective effect of MMI on myocardium ischemic rat model and H9c2 cells that underwent cell apoptosis induced by hypoxia. We established the myocardium ischemic rat model via the cardiac surgical procedures in vivo and treated the model rats with different concentration of MMI. In vitro , with the pretreatment of MMI for 12 h in the model of Na ₂S ₂O ₄-induced h ...
Timely disassembly of higher order protein-DNA complexes is essential for the proper control of biological processes. We became interested in protein disassembly when we found that the ClpX protein, a member of the Clp/Hsp100 family, promotes a protein-remodeling reaction that helps release the transposase from the DNA following recombination. This work contributed to the conclusion that Clp/Hsp100 proteins are chaperones specifically suited for disassembling or disaggregating other proteins. ClpX also functions as an essential component of the ClpXP protease. The ClpP component is a cylindrical protein chamber that provides the peptidase-active sites to degrade proteins that are chosen and delivered by ClpX. To increase our understanding of Clp/Hsp100 protein function, we are focusing on four research areas.. Protein processing during disassembly and degradation. Several groups studying Clp/Hsp100 proteins demonstrated that these enzymes have the capacity to unfold their substrate proteins ...
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Direct im aging of ROS in probe stained cells was carried out utilizing a fluorescence microscope, INK 128 価格 and photos had been captured with a DP Controller
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Mutations of the human valosin-containing protein gene cause autosomal-dominant inclusion body myopathy associated with Paget disease of bone and frontotemporal dementia. We identified strumpellin as a novel valosin-containing protein binding partner. Strumpellin mutations have been shown to cause hereditary spastic paraplegia. We demonstrate that strumpellin is a ubiquitously expressed protein present in cytosolic and endoplasmic reticulum cell fractions. Overexpression or ablation of wild-type strumpellin caused significantly reduced wound closure velocities in wound healing assays, whereas overexpression of the disease-causing strumpellin N471D mutant showed no functional effect. Strumpellin knockdown experiments in human neuroblastoma cells resulted in a dramatic reduction of axonal outgrowth. Knockdown studies in zebrafish revealed severe cardiac contractile dysfunction, tail curvature and impaired motility. The latter phenotype is due to a loss of central and peripheral motoneuron ...
TY - JOUR. T1 - DNA-RNA helicase activity of RAD3 protein of Saccharomyces cerevisiae. AU - Bailly, Véronique. AU - Sung, Patrick. AU - Prakash, Louise. AU - Prakash, Satya. PY - 1991. Y1 - 1991. N2 - The RAD3 gene of Saccharomyces cerevisiae is required for excision repair of UV-damaged DNA and is essential for cell viability. The RAD3 protein exhibits a remarkable degree of sequence homology to the human excision repair protein ERCC2. The RAD3 protein is a single-stranded DNA-dependent ATPase and a DNA helicase capable of denaturing long regions of duplex DNA. Here, we demonstrate that RAD3 also possesses a potent DNA-RNA helicase activity similar in efficiency to its DNA helicase activity. The rad3 Arg-48 mutant protein, which binds but does not hydrolyze ATP, lacks the DNA-RNA unwinding activity, indicating a dependence on ATP hydrolysis. RAD3 does not show any RNA-dependent NTPase activity and, as expected, does not unwind duplex RNA. This observation suggests that RAD3 translocates on DNA ...
A preparation of ATPase from the membranes of Micrococcus lysodeikticus, solubilized and more than 95% pure, showed two main bands in analytical polyacrylamide gel electrophoresis. They did not correspond to isoenzymes because one band could be converted into the other by exposure to a mildly alkaline pH value. The conversion was paralleled by changes in molecular weight, circular dichroism and catalytic properties. Denaturation by pH at 25 degrees C was followed by means of circular dichroism, ultracentrifugation and polyacrylamide gel electrophoresis. A large conformational transition took place in the acid range with midpoints at about pH = 3.6 (I = 10(-4) M), 4.3 (I = 0.03 M) and 5.3 (I = 0.1 M). The transition was irreversible. Strong aggregation of the protein occurred in this range of pH. The final product was largely random coil, but even at pH 1.5 dissociation into individual subunits was not complete. However, partial dissociation took place at pH 5 (I = 0.028 M). At this pH value the enzyme
Chromosome 1; KIF14 gene; KIAA0042 (alias); HUMORFW (alias); MGC142302 (alias); Genes Section; Adenosine Triphosphatases; Cytoskeletal Proteins; KIF14 protein, human; Kinesin; Microtubule Proteins; Microtubule-Associated Proteins; Molecular Motor Proteins; Neoplasm Proteins; Nerve Tissue Proteins; Digestive System Neoplasms; Eye Neoplasms; Liver Neoplasms; Neoplasms, Germ Cell and Embryonal; Neoplasms, Glandular and Epithelial; Neoplasms, Nerve Tissue; Neoplasms, Neuroepithelial; Neuroectodermal Tumors; ...
Bloom syndrome helicase (BLM) has key roles in homologous recombination repair, telomere maintenance, and DNA replication. Germ-line mutations in the BLM gene causes Bloom syndrome, a rare disorder characterized by premature aging and predisposition to multiple cancers, including breast cancer. The clinicopathologic significance of BLM in sporadic breast cancers is unknown. We investigated BLM mRNA expression in the Molecular Taxonomy of Breast Cancer International Consortium cohort (n = 1,950) and validated in an external dataset of 2,413 tumors. BLM protein level was evaluated in the Nottingham Tenovus series comprising 1,650 breast tumors. BLM mRNA overexpression was significantly associated with high histologic grade, larger tumor size, estrogen receptor-negative (ER(-)), progesterone receptor-negative (PR(-)), and triple-negative phenotypes (ps , 0.0001). BLM mRNA overexpression was also linked to aggressive molecular phenotypes, including PAM50.Her2 (P , 0.0001), PAM50.Basal (P , 0.0001), ...
Processivity is an essential feature of DNA replication. D. Jeruzalmi (New York) described loading of the β processivity clamp onto circular DNA in E. coli, which is accomplished by the clamp loader complex, composed of γ, δ, δ′, χ and ψ subunits. The major function of the AAA ATPase in the clamp loader complex is to partially dissociate a stable β dimer. γ and δ′ are both AAA proteins, but only γ is an active ATPase. Upon binding of ATP to γ, changes within the complex cause δ to bind β, a bi‐lobed dimer, and pry it partially open. DNA slips into the open dimer and, upon ATP hydrolysis, the complex dissociates and β closes, thus encircling the DNA.. The major features of bacterial DNA replication are recapitulated in mammals. In eukaryotes, DNA polymerase is stabilized on the DNA by an accessory sliding clamp, PCNA (proliferating cell nuclear antigen). PCNA is loaded on the DNA by a clamp loader, RFC (replication factor C). RFC is a pentameric ring structure consisting of ...
Research in my laboratory is aimed at the structural characterization of a class of proteins called transport ATPases. Transport ATPases are membrane bound enzymes, which catalyze the active transport of inorganic and organic molecules across biological membranes, a process vital to all forms of life. Many transport ATPases are key players in some of the most devastating human diseases such as cancer and AIDS. If we want to be able to fight these diseases on a molecular level (for example by drug design) we have to first understand the structure and mechanism of the disease causing proteins. Currently, we are working on two members of the transport ATPase family, the vacuolar ATPase (V-ATPase) and P-glycoprotein (Pgp; also called multi drug resistance protein). We are using cryo electron microscopy and protein NMR spectroscopy as well as biochemical and molecular biological techniques to obtain structural information for these two and related proteins ...
Heat shock 10 kDa protein 1 (Hsp10) also known as chaperonin 10 (cpn10) or early-pregnancy factor (EPF) is a protein that in humans is encoded by the HSPE1 gene. The homolog in E. coli is GroES that is a chaperonin which usually works in conjunction with GroEL. GroES exists as a ring-shaped oligomer of between six and eight identical subunits, while the 60 kDa chaperonin (cpn60 - or groEL in bacteria) forms a structure comprising 2 stacked rings, each ring containing 7 identical subunits. These ring structures assemble by self-stimulation in the presence of Mg2+-ATP. The central cavity of the cylindrical cpn60 tetradecamer provides an isolated environment for protein folding whilst cpn-10 binds to cpn-60 and synchronizes the release of the folded protein in an Mg2+-ATP dependent manner. The binding of cpn10 to cpn60 inhibits the weak ATPase activity of cpn60. Escherichia coli GroES has also been shown to bind ATP cooperatively, and with an affinity comparable to that of GroEL. Each GroEL subunit ...
TABLE-US-00022 TABLE 23 Pfam domain Accession Gathering name number cutoff Domain description 14-3-3 PF00244.9 25 14-3-3 protein 2OG-FeII_Oxy PF03171.10 11.5 2OG-Fe(II) oxygenase superfamily AAA PF00004.19 12.3 ATPase family associated with various cellular activities (AAA) AAA_2 PF07724.3 -5 ATPase family associated with various cellular activities (AAA) AAA_5 PF07728.4 4 ATPase family associated with various cellular activities (AAA) ABC2_membrane PF01061.13 -17.9 ABC-2 type transporter ABC_tran PF00005.16 9.5 ABC transporter ACP_syn_III_C PF08541.1 -24.4 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal ADH_N PF08240.2 -14.5 Alcohol dehydrogenase GroES-like domain ADH_zinc_N PF00107.16 23.8 Zinc-binding dehydrogenase AOX PF01786.8 25 Alternative oxidase AP2 PF00847.10 0 AP2 domain AT_hook PF02178.8 3.6 AT hook motif Aa_trans PF01490.7 -128.4 Transmembrane amino acid transporter protein Abhydrolase_1 PF00561.10 10.3 alpha/beta hydrolase fold Acid_phosphat_A PF00328.12 -64.5 ...
ENCODES a protein that exhibits ATPase activity (ortholog); proton-transporting ATP synthase activity, rotational mechanism (ortholog); INVOLVED IN response to hyperoxia; mitochondrial ATP synthesis coupled proton transport (ortholog); ASSOCIATED WITH Reperfusion Injury; Acute Liver Failure (ortholog); Apical Hypertrophic Cardiomyopathy and Neuropathy (ortholog); FOUND IN mitochondrial proton-transporting ATP synthase complex; INTERACTS WITH 3-chloropropane-1,2-diol; apigenin; atrazine
Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single-stranded and primed DNAs and has weak ATPase activity that is stimulated by the presence of primed DNA, replication protein A (RPA) and by proliferating cell nuclear antigen (PCNA). The CTF18-RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. It also interacts with and stimulates DNA polymerase POLH ...
Reactivité: Humain, Souris Hôte: Lapin Clone: Polyclonal Conjugué: HRP | Commandez Blm/Blooms Syndrome Protein Blm lanticorps (ABIN1712183).
Menkes Disease is a genetic disorder affecting the metabolism of copper. Patient with this disease are both physically and mentally retarded. Menkes disease is usually first detected in the first 2-3 months of life. Infant males born with the disease fail to thrive, experience hypothermia, have delayed development, and experience seizures. These infants also have characteristic physical features such as changes of their hair and face. Females may also have changes in hair and skin color, but rarely have significant medical problems.. Appropriate treatment of Menkes Disease requires that the disease be diagnosed early and treatment started before irreversible brain damage occurs. The aim of treatment is to bypass the normal route of absorption of copper through the gastrointestinal tract. Copper must then be delivered to brain cells and be available for use by enzymes.. Copper histidine is a copper replacement that can be injected directly into the body to avoid absorption through the ...
In the previously determined structure of mitochondrial F1-ATPase determined with crystals grown in the presence of adenylyl-imidodiphosphate (AMP-PNP) and ADP, the three catalytic beta-subunits have different conformations and nucleotide occupancies. AMP-PNP and ADP are bound to subunits beta TP and beta DP, respectively, and the third beta-subunit (beta E) has no bound nucleotide. The efrapeptins are a closely related family of modified linear peptides containing 15 amino acids that inhibit both ATP synthesis and hydrolysis by binding to the F1 catalytic domain of F1F0-ATP synthase. In crystals of F1-ATPase grown in the presence of both nucleotides and inhibitor, efrapeptin is bound to a unique site in the central cavity of the enzyme. Its binding is associated with small structural changes in side chains of F1-ATPase around the binding pocket. Efrapeptin makes hydrophobic contacts with the alpha-helical structure in the gamma-subunit, which traverses the cavity, and with subunit beta E and ...
ATPase activity (E.C. 3.6.1.3) has been studied by electron microscopy with the help of several cytochemical techniques on Eigenmannia virescenselectrocytes. Incubation was carried out with in two...
Introduction: Alzheimers disease (AD) is a neurodegenerative disorder, clinically characterized by memory dysfunction and progressive loss of cognition. No curative therapeutic or drug is available for the complete cure of this disease. The present study was aimed to evaluate the efficacy of Lactobacillus plantarum MTCC1325 in ATPases activity in the selected brain regions of rats induced with Alzheimers. Methods: For the study, 48 healthy Wistar rats were divided into four groups: group I as control group, group II as AD model (AD induced by intraperitoneal injection of D-Galactose, 120 mg/kg body weight for 6 weeks), group III as normal control rats which were orally administered only with L. plantarum MTCC1325 for 60 days, and group IV where the AD-induced rats simultaneously received oral treatment of L. plantarum MTCC1325 (10ml/kg body weight, 12×108 CFU/mL) for 60 days. The well known membrane bound transport enzymes including Na+, K+-ATPases, Ca2+-ATPases, and Mg2+-ATPases were assayed in the
Approximately 2000 kinases are known and more than 90 Protein Tyrosine Kinases (PTKs) have been found in the human genome. They are divided into two classes, receptor and non-receptor PTKs. At present, 58 receptor tyrosine kinases (RTKs) are known, grouped into 20 subfamilies. They play pivotal roles in diverse cellular activities including growth, differentiation, metabolism, adhesion, motility, death [1]. RTKs are composed of an extracellular domain, which is able to bind a specific ligand, a transmembrane domain, and an intracellular catalytic domain, which is able to bind and phosphorylate selected substrates. Binding of a ligand to the extracellular region causes a series of structural rearrangements in the RTK that lead to its enzymatic activation. In particular, movement of some parts of the kinase domain gives free access to adenosine triphosphate (ATP) and the substrate to the active site. This triggers a cascade of events through phosphorylation of intracellular proteins that ...
Active medical nanorobots [1, 2] maneuvering inside living cells could disturb or disrupt any of the many functions of the cytoskeleton. The cytoskeleton is the internal structural framework of a cell, consisting of several different types of filaments (see below). The functions of the cytoskeleton include: (1) mechanical support, such as cell movement, adhesive interaction with the extracellular matrix (ECM) and neighboring cells, and stabilization of cell shape including cellular "tensegrity"; (2) integration of diverse cellular activities, such as intracellular movement including transport and positioning to the appropriate locations of organelles, intracellular particles, RNA and proteins; and (3) intracellular signal transduction, which includes structural information regarding cellular origin and differentiation state [3]. In diverse cell types, microtubule and actin filament networks cooperate functionally during many processes, such as vesicle and organelle transport, cleavage furrow ...
Complete information for BLM gene (Protein Coding), Bloom Syndrome RecQ Like Helicase, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Reaktivität: Human, Säugetier, Maus and more. 76 verschiedene RECQL2 Antikörper vergleichen. Alle direkt auf antikörper-online bestellbar!
Figure 4. The mature domain-binding site onto SecA. (A) The E. coli SecA (gray)-SeYEG (yellow) was modeled after the Thermotoga maritima translocase in three conformational states, based on PBD (purple) positioning: closed (left), open (middle), and wide open (right). Side and bottom views are shown (as indicated). I, II: SecA clamps. (B) Kd measurements of PhoA and its signal peptide (SPPhoA) for the wild-type (WT), locked closed (LC), locked open (LO), and locked wide open (LWO) SecA bound to SecYEG-inverted membrane vesicles. proPhoA(1-30) was used as SPPhoA. Affinity values represent means ± SEM; n = 3. (C) Potential space occupied by an incoming preprotein onto the cytoplasmic side (platform) of a SecA(gray)-SecYEG(yellow) translocase; signal peptide is in green. The inner circle represents the minimum area a translocation-competent preprotein would occupy, depicted here by the predicted DH of the smallest known preprotein (proEcnA; ∼3 nm; Table S8). The bigger circle represents the area ...
After cellular uptake, Copper (Cu) ions are transferred from the chaperone Atox1 to the Wilson disease protein (ATP7B) for incorporation into Cu-dependent enzymes in the secretory pathway. Human ATP7B is a large multi-domain membrane-spanning protein which, in contrast to homologues in other organisms, has six similar cytoplasmic metal-binding domains (MBDs). The reason for multiple MBDs is proposed to be indirect modulation of enzymatic activity and it is thus intriguing that point mutations in MBDs can promote Wilson disease. We here investigated, in vitro and in silico, the biophysical consequences of clinically-observed Wilson disease mutations, G85V in MBD1 and G591D in MBD6, incorporated in domain 4. Because G85 and G591 correspond to a conserved Gly found in all MBDs, we introduced the mutations in the well-characterized MBD4. We found the mutations to dramatically reduce the MBD4 thermal stability, shifting the midpoint temperature of unfolding by more than 20 A degrees C. In contrast to ...
TY - JOUR. T1 - Xanthohumol impairs autophagosome maturation through direct inhibition of valosin-containing protein. AU - Sasazawa, Yukiko. AU - Kanagaki, Shuhei. AU - Tashiro, Etsu. AU - Nogawa, Toshihiko. AU - Muroi, Makoto. AU - Kondoh, Yasumitsu. AU - Osada, Hiroyuki. AU - Imoto, Masaya. PY - 2012/5/18. Y1 - 2012/5/18. N2 - Autophagy is a bulk, nonspecific protein degradation pathway that is involved in the pathogenesis of cancer and neurodegenerative disease. Here, we observed that xanthohumol (XN), a prenylated chalcone present in hops (Humulus lupulus L.) and beer, modulates autophagy. By using XN-immobilized beads, valosin-containing protein (VCP) was identified as a XN-binding protein. VCP has been reported to be an essential protein for autophagosome maturation. Using an in vitro pull down assay, we showed that XN bound directly to the N domain, which is known to mediate cofactor and substrate binding to VCP. These data indicated that XN inhibited the function of VCP, thereby allowing ...
Copper is an essential metal but its overload causes Wilson disease, a disorder due to mutations in copper transporter ATP7B. To remove excess copper into the bile, ATP7B traffics toward canalicular area of hepatocytes. However, the trafficking mechanisms of ATP7B remain elusive. Polishchuk and colleagues show that, in response to elevated copper, ATP7B moves from the Golgi to lysosomes and imports metal into their lumen. ATP7B enables lysosomes to undergo exocytosis through the interaction with p62 subunit of dynactin that allows lysosome translocation toward the canalicular pole of hepatocytes. Activation of lysosomal exocytosis stimulates copper clearance from the hepatocytes and rescues the most frequent Wilson-disease-causing ATP7B mutant to the appropriate functional site. This findings indicate that lysosomes serve as an important intermediate in ATP7B trafficking, whereas lysosomal exocytosis operates as an integral process in copper excretion and hence can be targeted for therapeutic ...
Background. Karwinskia humboldtiana currently known by the common names buckthorn, wild cherry, tullidora, capulin tullidor, capulincillo, coyotillo, and cacatsin, is a poisonous shrub of the Rhamnaceae family that grows from Southern United States to northern Colombia [1]. Medicinal properties have been attributed to several species of the genus Karwisnkia due to a secondary metabolite with antineo-plastic action on mammalian tumor cells [2].. The ingestion of the green or ripe fruit of Kh causes a flaccid, symmetric, progressive, and ascending palsy of the lower limbs, which, in severe cases may progress to quadriplegia and breathing insufficiency, with victims requiring assisted mechanical ventilation. The neurologic symptoms are similar to those of poliomyelitis, Guillain-Barre syndrome, and other polyradiculoneuritis syndromes, for which it is often mistaken. In Mexico, the accidental ingestion of Kh causes several health problems [3-5], particularly there are numerous reports of poisoning ...
1. Muscle fibres may be subdivided into type I (with slow-twitch contractile properties) and type II (fast-twitch) depending on their myosin adenosine triphosphatase activity. In voluntary isometric contractions type I fibres are utilized at low forces (,20% of maximum) whereas type II fibres are recruited in addition at high forces. This physiological recruitment order has enabled us to measure the relaxation rate of type I and II fibres in vivo in normal human subjects.. 2. Relaxation rate was measured in 16 subjects from low (10% of maximum) and maximum isometric quadriceps contractions and the muscle-fibre type composition determined from needle-biopsy specimens in 10 subjects. The relaxation rate of type II fibres was calculated to be twice as fast as that of type I.. 3. It was not possible to estimate, from studies in 33 quadriceps muscles (25 normal subjects), the contribution of type II fibres to overall fibre area from the relaxation rate as determined from electrically stimulated ...
Looking for online definition of ATP-dependent RNA helicase eIF4A-3 in the Medical Dictionary? ATP-dependent RNA helicase eIF4A-3 explanation free. What is ATP-dependent RNA helicase eIF4A-3? Meaning of ATP-dependent RNA helicase eIF4A-3 medical term. What does ATP-dependent RNA helicase eIF4A-3 mean?

Phosphate binding by cerebral microsomes in relation to adenosine-triphosphatase activity | Biochemical JournalPhosphate binding by cerebral microsomes in relation to adenosine-triphosphatase activity | Biochemical Journal

Phosphate binding by cerebral microsomes in relation to adenosine-triphosphatase activity. R Rodnight, DA Hems, BE Lavin ... Phosphate binding by cerebral microsomes in relation to adenosine-triphosphatase activity Message Subject (Your Name) has ...
more infohttp://www.biochemj.org/content/101/2/502

Interactions of Phospholipids with Sodium-plus-Potassium Ion-Dependent Adenosine Triphosphatase | Biochemical Society...Interactions of Phospholipids with Sodium-plus-Potassium Ion-Dependent Adenosine Triphosphatase | Biochemical Society...

Interactions of Phospholipids with Sodium-plus-Potassium Ion-Dependent Adenosine Triphosphatase MARTA ISERN DE CALDENTEY MARTA ... The Adenosine Triphosphatase Reactions of Myosin and Actomyosin and their Relation to Energy Transduction in Muscle Biochem Soc ... Variation in Adenosine Triphosphatase Activities in Serial Preparations of Human Erythrocyte Membranes Biochem Soc Trans ( ... Requirements for the Inhibition of Sodium and Potassium Ion-Transporting Adenosine Triphosphatase by Adriamycin Biochem Soc ...
more infohttps://portlandpress.com/biochemsoctrans/article-abstract/5/1/107/89034/Interactions-of-Phospholipids-with-Sodium-plus?redirectedFrom=fulltext

Vectorial aspects of adenosine-triphosphatase activity in erythrocyte membranes | Biochemical JournalVectorial aspects of adenosine-triphosphatase activity in erythrocyte membranes | Biochemical Journal

Vectorial aspects of adenosine-triphosphatase activity in erythrocyte membranes. R Whittam, ME Ager ... Vectorial aspects of adenosine-triphosphatase activity in erythrocyte membranes Message Subject (Your Name) has forwarded a ...
more infohttp://www.biochemj.org/content/93/2/337

Recombinant Human FHIT protein (ab95856) | AbcamRecombinant Human FHIT protein (ab95856) | Abcam

FHIT (fragile histidine triad) cleaves adenosine 5 PPP 5 A to yield AMP and ADP. Alterations and deletions of the FHIT gene ... Bis 5 adenosyl triphosphatase. *Bis(5 adenosyl) triphosphatase. *Diadenosine 5 5 P1 P3 triphosphate hydrolase ...
more infohttp://www.abcam.com/recombinant-human-fhit-protein-ab95856.html

Category:Adenosine triphosphatases - Wikimedia CommonsCategory:Adenosine triphosphatases - Wikimedia Commons

Media in category "Adenosine triphosphatases". The following 74 files are in this category, out of 74 total. ... Retrieved from "https://commons.wikimedia.org/w/index.php?title=Category:Adenosine_triphosphatases&oldid=131322728" ...
more infohttps://commons.wikimedia.org/wiki/Category:Adenosine_triphosphatases

Adenosine triphosphatase | enzyme | Britannica.comAdenosine triphosphatase | enzyme | Britannica.com

Other articles where Adenosine triphosphatase is discussed: cell: The sodium-potassium pump: An enzyme called sodium-potassium- ... www.britannica.com/science/adenosine-triphosphatase", "title": "Adenosine triphosphatase", "documentGroup": "TOPIC PAGINATED ... adenosine triphosphate (ATP)Adenosine triphosphate, or ATP, is the primary carrier of energy in cells. The water-mediated ...
more infohttps://www.britannica.com/science/adenosine-triphosphatase

Na+, K(+)-adenosine triphosphatase regulation in hypertrophied vascular smooth muscle cells. | HypertensionNa+, K(+)-adenosine triphosphatase regulation in hypertrophied vascular smooth muscle cells. | Hypertension

Na+, K(+)-adenosine triphosphatase regulation in hypertrophied vascular smooth muscle cells.. L M Krug, B C Berk ...
more infohttp://hyper.ahajournals.org/content/20/2/144

Organization of Oligomycin-Sensitive Adenosine Triphosphatase | Biochemical Society TransactionsOrganization of Oligomycin-Sensitive Adenosine Triphosphatase | Biochemical Society Transactions

Organization of Oligomycin-Sensitive Adenosine Triphosphatase. M. D. PARTIS, E. BERTOLI, S. MASCARELLO, D. E. GRIFFITHS ... Organization of Oligomycin-Sensitive Adenosine Triphosphatase Message Subject (Your Name) has forwarded a page to you from ...
more infohttp://www.biochemsoctrans.org/content/4/1/88

Electrophorus Adenosine Triphosphatase: Sodium-Activated Exchange after N-Ethyl Maleimide Treatment | ScienceElectrophorus Adenosine Triphosphatase: Sodium-Activated Exchange after N-Ethyl Maleimide Treatment | Science

Electrophorus Adenosine Triphosphatase: Sodium-Activated Exchange after N-Ethyl Maleimide Treatment. By Stanley Fahn, R. Wayne ... Electrophorus Adenosine Triphosphatase: Sodium-Activated Exchange after N-Ethyl Maleimide Treatment. By Stanley Fahn, R. Wayne ... Electrophorus Adenosine Triphosphatase: Sodium-Activated Exchange after N-Ethyl Maleimide Treatment Message Subject. (Your Name ... The same preparation catalyzes a Mg++-dependent transphosphorylation between adenosine triphosphate and adenosine diphosphate. ...
more infohttps://science.sciencemag.org/content/145/3629/283.abstract

Symptoms of Adenosine triphosphatase deficiency, anaemia due to - RightDiagnosis.comSymptoms of Adenosine triphosphatase deficiency, anaemia due to - RightDiagnosis.com

... and correct diagnosis for Adenosine triphosphatase deficiency, anaemia due to signs or Adenosine triphosphatase deficiency, ... anaemia due to including 12 medical symptoms and signs of Adenosine triphosphatase deficiency, anaemia due to, alternative ... Do I have Adenosine triphosphatase deficiency, anaemia due to? *Adenosine triphosphatase deficiency, anaemia due to: ... Research symptoms & diagnosis of Adenosine triphosphatase deficiency, anaemia due to: *Overview -- Adenosine triphosphatase ...
more infohttps://www.rightdiagnosis.com/a/adenosine_triphosphatase_deficiency_anaemia_due_to/symptoms.htm

The cytochemical demonstration of NaK dependent adenosine triphosphatase at electrocyte level in Eigenmannia virescens ...The cytochemical demonstration of NaK dependent adenosine triphosphatase at electrocyte level in Eigenmannia virescens ...

Effect of adenosine triphosphatase hydrolysis by lead ion on the histochemical localization of adenosine triphosphatase ... Sodium potassium activated adenosine triphosphatase. VII. Concurent inhibition of Na+K+ adenosine triphosphatase and activation ... Denizot JP (1980) Adenosine triphosphatases (ATP-A) on sensory cell plasmic membranes of tuberous organs (electroreceptors) of ... Siegel GJ, Fogt SM (1977) Inhibition by lead ion of Electrophorus electroplax (Na+−K+)-adenosine triphosphatase and K+-p- ...
more infohttps://link.springer.com/article/10.1007/BF00495831

Erythrocyte and plasma Ca2+, Mg2+ and cell membrane adenosine triphosphatase activity in patients with essential hypertension. ...Erythrocyte and plasma Ca2+, Mg2+ and cell membrane adenosine triphosphatase activity in patients with essential hypertension. ...

... To assess the relationships between plasma and intracellular Ca2+, Mg2+ and blood cell membrane adenosine triphosphatase ( ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/10374376

PRIME PubMed | Pathogenesis of post-thymectomy autoimmune gastritis. Identification of anti-H/K adenosine triphosphatase...PRIME PubMed | Pathogenesis of post-thymectomy autoimmune gastritis. Identification of anti-H/K adenosine triphosphatase...

Identification of anti-H/K adenosine triphosphatase-reactive T cell were found in PRIME PubMed. Download Prime PubMed App to ... Identification of anti-H/K adenosine triphosphatase-reactive T cells. Journal of Immunology (Baltimore, Md. : 1950), 157(4), pp ... Identification of anti-H/K Adenosine Triphosphatase-reactive T Cells." Journal of Immunology (Baltimore, Md. : 1950), vol. 157 ... Identification of anti-H/K adenosine triphosphatase-reactive T cells. AU - Suri-Payer,E, AU - Kehn,P J, AU - Cheever,A W, AU - ...
more infohttps://www.unboundmedicine.com/medline/citation/8759770/Pathogenesis_of_post_thymectomy_autoimmune_gastritis__Identification_of_anti_H/K_adenosine_triphosphatase_reactive_T_cells_

Erythrocyte Sodium-plus-Potassium Ion-Activated Adenosine Triphosphatase in Depressive Patients | Biochemical Society...Erythrocyte Sodium-plus-Potassium Ion-Activated Adenosine Triphosphatase in Depressive Patients | Biochemical Society...

Erythrocyte Sodium-plus-Potassium Ion-Activated Adenosine Triphosphatase in Depressive Patients. D. A. T. DICK, G. J. NAYLOR, E ... Erythrocyte Sodium-plus-Potassium Ion-Activated Adenosine Triphosphatase in Depressive Patients. D. A. T. DICK, G. J. NAYLOR, E ... Erythrocyte Sodium-plus-Potassium Ion-Activated Adenosine Triphosphatase in Depressive Patients Message Subject (Your Name) has ...
more infohttp://www.biochemsoctrans.org/content/2/3/505

Arsenite-resistant Leishmania donovani promastigotes express an enhanced membrane P-type adenosine triphosphatase activity that...Arsenite-resistant Leishmania donovani promastigotes express an enhanced membrane P-type adenosine triphosphatase activity that...

The membrane-associated P-type adenosine triphosphatase (ATPase) activity detected in crude membrane fractions of the resistant ... Arsenite-resistant Leishmania donovani promastigotes express an enhanced membrane P-type adenosine triphosphatase activity that ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/10952266?dopt=Abstract

Sodium-potassium dependent adenosine triphosphatase activity in gills and kidneys of Atlantic salmon (|i|Salmo salar|/i|)Sodium-potassium dependent adenosine triphosphatase activity in gills and kidneys of Atlantic salmon (|i|Salmo salar|/i|)

Sodium-potassium dependent adenosine triphosphatase activity in gills and kidneys of Atlantic salmon (Salmo salar). Series ... Sodium-potassium dependent adenosine triphosphatase activity in gills and kidneys of Atlantic salmon (Salmo salar). Comparative ...
more infohttps://pubs.er.usgs.gov/publication/1013997

Inhibition of Brain Sodium- and Potassium-Stimulated Adenosine Triphosphatase Activity by Chlorpromazine Free Radical |...Inhibition of Brain Sodium- and Potassium-Stimulated Adenosine Triphosphatase Activity by Chlorpromazine Free Radical |...

Inhibition of Brain Sodium- and Potassium-Stimulated Adenosine Triphosphatase Activity by Chlorpromazine Free Radical. TAI ... Inhibition of Brain Sodium- and Potassium-Stimulated Adenosine Triphosphatase Activity by Chlorpromazine Free Radical. TAI ... Inhibition of Brain Sodium- and Potassium-Stimulated Adenosine Triphosphatase Activity by Chlorpromazine Free Radical. TAI ... Inhibition of Brain Sodium- and Potassium-Stimulated Adenosine Triphosphatase Activity by Chlorpromazine Free Radical ...
more infohttp://molpharm.aspetjournals.org/content/4/6/600

Possible mechanism for the inhibition of gastric (H+ + K+)-adenosine triphosphatase by the proton pump inhibitor AG-1749. |...Possible mechanism for the inhibition of gastric (H+ + K+)-adenosine triphosphatase by the proton pump inhibitor AG-1749. |...

Possible mechanism for the inhibition of gastric (H+ + K+)-adenosine triphosphatase by the proton pump inhibitor AG-1749.. H ... Possible mechanism for the inhibition of gastric (H+ + K+)-adenosine triphosphatase by the proton pump inhibitor AG-1749.. H ... Possible mechanism for the inhibition of gastric (H+ + K+)-adenosine triphosphatase by the proton pump inhibitor AG-1749.. H ... Possible mechanism for the inhibition of gastric (H+ + K+)-adenosine triphosphatase by the proton pump inhibitor AG-1749. ...
more infohttp://jpet.aspetjournals.org/content/248/2/799

Get PDF - Electron microscopic studies on renal tubules (Malpighian tubules) in Drosophila melanogaster. V. Localization of Na...Get PDF - Electron microscopic studies on renal tubules (Malpighian tubules) in Drosophila melanogaster. V. Localization of Na...

Studies on the anion-sensitivity, oligomycin-sensitivity and sub-cellular localization of adenosine triphosphatase activity in ... V. Localization of Na-adenosine triphosphatase. Electron microscopic studies on renal tubules (Malpighian tubules) in ... V. Localization of Na-adenosine triphosphatase. Schulte, E.. Zeitschrift für Zellforschung und Mikroskopische Anatomie 133(1): ...
more infohttps://eurekamag.com/research/042/983/042983609.php

Lithium and Rubidium Interactions with Sodium- and Potassium-Dependent Adenosine Triphosphatase: A Molecular Basis for the...Lithium and Rubidium Interactions with Sodium- and Potassium-Dependent Adenosine Triphosphatase: A Molecular Basis for the...

Lithium and Rubidium Interactions with Sodium- and Potassium-Dependent Adenosine Triphosphatase: A Molecular Basis for the ... Lithium and Rubidium Interactions with Sodium- and Potassium-Dependent Adenosine Triphosphatase: A Molecular Basis for the ... Lithium and Rubidium Interactions with Sodium- and Potassium-Dependent Adenosine Triphosphatase: A Molecular Basis for the ... Lithium and Rubidium Interactions with Sodium- and Potassium-Dependent Adenosine Triphosphatase: A Molecular Basis for the ...
more infohttp://molpharm.aspetjournals.org/content/10/3/501

LOCALIZATION OF (SODIUM ION, POTASSIUM ION)-ACTIVATED ADENOSINE TRIPHOSPHATASE ACTIVITY IN TELEOST CHLORIDE CELLS: ...LOCALIZATION OF (SODIUM ION, POTASSIUM ION)-ACTIVATED ADENOSINE TRIPHOSPHATASE ACTIVITY IN TELEOST CHLORIDE CELLS: ...

LOCALIZATION OF (SODIUM ION, POTASSIUM ION)-ACTIVATED ADENOSINE TRIPHOSPHATASE ACTIVITY IN TELEOST CHLORIDE CELLS: CYTOCHEMICAL ...
more infohttps://scholarship.rice.edu/handle/1911/15373

Catalytic site cooperativity of beef heart mitochondrial F1 adenosine triphosphatase--correlation of initial velocity, bound...Catalytic site cooperativity of beef heart mitochondrial F1 adenosine triphosphatase--correlation of initial velocity, bound...

Catalytic site cooperativity of beef heart mitochondrial F1 adenosine triphosphatase--correlation of initial velocity, bound ...
more infohttps://openagricola.nal.usda.gov/catalog/IND82118729

Subunit composition, function, and spatial arrangement in the Ca2+-and Mg2+-activated adenosine triphosphatases of Escherichia...Subunit composition, function, and spatial arrangement in the Ca2+-and Mg2+-activated adenosine triphosphatases of Escherichia...

... and spatial arrangement in the Ca2+-and Mg2+-activated adenosine triphosphatases of Escherichia coli and Salmonella typhimurium ... Subunit composition, function, and spatial arrangement in the Ca2+-and Mg2+-activated adenosine triphosphatases of Escherichia ... and spatial arrangement in the Ca2+-and Mg2+-activated adenosine triphosphatases of Escherichia coli and Salmonella typhimurium ...
more infohttps://www.semanticscholar.org/paper/Subunit-composition%2C-function%2C-and-spatial-in-the-Bragg-Hou/2a59bc3dd632e6fa5b4edca33fe7a616efff5244

Adenosine triphosphatase test synonyms, adenosine triphosphatase test antonyms - FreeThesaurus.comAdenosine triphosphatase test synonyms, adenosine triphosphatase test antonyms - FreeThesaurus.com

Antonyms for adenosine triphosphatase test. 2 words related to adenosine: biochemistry, nucleoside. What are synonyms for ... Synonyms for adenosine triphosphatase test in Free Thesaurus. ... redirected from adenosine triphosphatase test). Also found in: ... Related to adenosine triphosphatase test: adenosine triphosphate, adenosine stress test #vtZoom,.vt-link{cursor:pointer} .vt- ... Adenosine triphosphatase test synonyms, adenosine triphosphatase test antonyms - FreeThesaurus.com https://www.freethesaurus. ...
more infohttp://www.freethesaurus.com/adenosine+triphosphatase+test
  • article{Bragg1975SubunitCF, title={Subunit composition, function, and spatial arrangement in the Ca2+-and Mg2+-activated adenosine triphosphatases of Escherichia coli and Salmonella typhimurium. (semanticscholar.org)