5'-Adenylic acid, monoanhydride with sulfuric acid. The initial compound formed by the action of ATP sulfurylase on sulfate ions after sulfate uptake. Synonyms: adenosine sulfatophosphate; APS.
An enzyme that catalyzes the activation of sulfate ions by ATP to form adenosine-5'-phosphosulfate and pyrophosphate. This reaction constitutes the first enzymatic step in sulfate utilization following the uptake of sulfate. EC 2.7.7.4.
A nucleoside that is composed of ADENINE and D-RIBOSE. Adenosine or adenosine derivatives play many important biological roles in addition to being components of DNA and RNA. Adenosine itself is a neurotransmitter.
3'-Phosphoadenosine-5'-phosphosulfate. Key intermediate in the formation by living cells of sulfate esters of phenols, alcohols, steroids, sulfated polysaccharides, and simple esters, such as choline sulfate. It is formed from sulfate ion and ATP in a two-step process. This compound also is an important step in the process of sulfur fixation in plants and microorganisms.
A subclass of adenosine A2 receptors found in LEUKOCYTES, the SPLEEN, the THYMUS and a variety of other tissues. It is generally considered to be a receptor for ADENOSINE that couples to the GS, STIMULATORY G-PROTEIN.
A subtype of ADENOSINE RECEPTOR that is found expressed in a variety of tissues including the BRAIN and DORSAL HORN NEURONS. The receptor is generally considered to be coupled to the GI, INHIBITORY G-PROTEIN which causes down regulation of CYCLIC AMP.
An enzyme that catalyzes the hydrolysis of ADENOSINE to INOSINE with the elimination of AMMONIA.
A subtype of ADENOSINE RECEPTOR that is found expressed in a variety of locations including the BRAIN and endocrine tissues. The receptor is generally considered to be coupled to the GI, INHIBITORY G-PROTEIN which causes down regulation of CYCLIC AMP.
A subclass of adenosine A2 receptors found in the CECUM, the COLON, the BLADDER, and a variety of other tissues. It is generally considered to be a low affinity receptor for ADENOSINE that couples to the GS, STIMULATORY G-PROTEIN.
An enzyme that catalyzes the formation of ADP plus AMP from adenosine plus ATP. It can serve as a salvage mechanism for returning adenosine to nucleic acids. EC 2.7.1.20.
A subclass of ADENOSINE RECEPTORS that are generally considered to be coupled to the GS, STIMULATORY G-PROTEIN which causes up regulation of CYCLIC AMP.
Compounds that selectively bind to and activate ADENOSINE A2 RECEPTORS.
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A plant genus of the family FABACEAE. Some species were reclassified from CASSIA and Senna.
K-Cl cotransporter ubiquitously expressed with higher expression levels in ERYTHROCYTES of ANEMIA, SICKLE CELL. It mediates active potassium and chloride cotransport across the plasma membrane and contributes to cell volume homeostasis
An enzyme that catalyzes reversibly the oxidation of an aldose to an alditol. It possesses broad specificity for many aldoses. EC 1.1.1.21.
Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.
Na-Cl cotransporter in the convoluted segments of the DISTAL KIDNEY TUBULE. It mediates active reabsorption of sodium and chloride and is inhibited by THIAZIDE DIURETICS.
Databases devoted to knowledge about specific genes and gene products.
The complete genetic complement contained in the DNA of a set of CHROMOSOMES in a HUMAN. The length of the human genome is about 3 billion base pairs.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
A large group of bacteria including those which oxidize ammonia or nitrite, metabolize sulfur and sulfur compounds, or deposit iron and/or manganese oxides.
An element that is a member of the chalcogen family. It has an atomic symbol S, atomic number 16, and atomic weight [32.059; 32.076]. It is found in the amino acids cysteine and methionine.
Inorganic or organic compounds that contain sulfur as an integral part of the molecule.
Hot springs on the ocean floor. They are commonly found near volcanically active places such as mid-oceanic ridges.
A phylum of gram-negative bacteria containing seven class-level groups from a wide variety of environments. Most members are chemoheterotrophs.
A group of proteobacteria consisting of chemoorganotrophs usually associated with the DIGESTIVE SYSTEM of humans and animals.
A group of the proteobacteria comprised of facultatively anaerobic and fermentative gram-negative bacteria.
Plant cell inclusion bodies that contain the photosynthetic pigment CHLOROPHYLL, which is associated with the membrane of THYLAKOIDS. Chloroplasts occur in cells of leaves and young stems of plants. They are also found in some forms of PHYTOPLANKTON such as HAPTOPHYTA; DINOFLAGELLATES; DIATOMS; and CRYPTOPHYTA.
The inherent or induced capacity of plants to withstand or ward off biological attack by pathogens.
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
An excited state of molecular oxygen generated photochemically or chemically. Singlet oxygen reacts with a variety of biological molecules such as NUCLEIC ACIDS; PROTEINS; and LIPIDS; causing oxidative damages.
A large multisubunit protein complex that is found in the THYLAKOID MEMBRANE. It uses light energy derived from LIGHT-HARVESTING PROTEIN COMPLEXES to drive electron transfer reactions that result in either the reduction of NADP to NADPH or the transport of PROTONS across the membrane.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
The process by which ELECTRONS are transported from a reduced substrate to molecular OXYGEN. (From Bennington, Saunders Dictionary and Encyclopedia of Laboratory Medicine and Technology, 1984, p270)
Enzymes which transfer sulfate groups to various acceptor molecules. They are involved in posttranslational sulfation of proteins and sulfate conjugation of exogenous chemicals and bile acids. EC 2.8.2.
Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES.

Reduction of adenosine-5'-phosphosulfate instead of 3'-phosphoadenosine-5'-phosphosulfate in cysteine biosynthesis by Rhizobium meliloti and other members of the family Rhizobiaceae. (1/37)

We have cloned and sequenced three genes from Rhizobium meliloti (Sinorhizobium meliloti) that are involved in sulfate activation for cysteine biosynthesis. Two of the genes display homology to the Escherichia coli cysDN genes, which code for an ATP sulfurylase (EC 2.7.7.4). The third gene has homology to the E. coli cysH gene, a 3'-phosphoadenosine-5'-phosphosulfate (PAPS) reductase (EC 1.8.99.4), but has greater homology to a set of genes found in Arabidopsis thaliana that encode an adenosine-5'-phosphosulfate (APS) reductase. In order to determine the specificity of the R. meliloti reductase, the R. meliloti cysH homolog was histidine tagged and purified, and its specificity was assayed in vitro. Like the A. thaliana reductases, the histidine-tagged R. meliloti cysH gene product appears to favor APS over PAPS as a substrate, with a Km for APS of 3 to 4 microM but a Km for PAPS of >100 microM. In order to determine whether this preference for APS is unique to R. meliloti among members of the family Rhizobiaceae or is more widespread, cell extracts from R. leguminosarum, Rhizobium sp. strain NGR234, Rhizobium fredii (Sinorhizobium fredii), and Agrobacterium tumefaciens were assayed for APS or PAPS reductase activity. Cell extracts from all four species also preferentially reduce APS over PAPS.  (+info)

Identification of a new class of 5'-adenylylsulfate (APS) reductases from sulfate-assimilating bacteria. (2/37)

A gene was cloned from Burkholderia cepacia DBO1 that is homologous with Escherichia coli cysH encoding 3'-phosphoadenylylsulfate (PAPS) reductase. The B. cepacia gene is the most recent addition to a growing list of cysH homologs from a diverse group of sulfate-assimilating bacteria whose products show greater homology to plant 5'-adenylylsulfate (APS) reductase than they do to E. coli CysH. The evidence reported here shows that the cysH from one of the species, Pseudomonas aeruginosa, encodes APS reductase. It is able to complement an E. coli cysH mutant and a cysC mutant, indicating that the enzyme is able to bypass PAPS, synthesized by the cysC product. Insertional knockout mutation of P. aeruginosa cysH produced cysteine auxotrophy, indicating its role in sulfate assimilation. Purified P. aeruginosa CysH expressed as a His-tagged recombinant protein is able to reduce APS, but not PAPS. The enzyme has a specific activity of 5.8 micromol. min(-1). mg of protein(-1) at pH 8.5 and 30 degrees C with thioredoxin supplied as an electron donor. APS reductase activity was detected in several bacterial species from which the novel type of cysH has been cloned, indicating that this enzyme may be widespread. Although an APS reductase from dissimilatory sulfate-reducing bacteria is known, it shows no structural or sequence homology with the assimilatory-type APS reductase reported here. The results suggest that the dissimilatory and assimilatory APS reductases evolved convergently.  (+info)

Effects of AMP derivatives on cyclic AMP levels in NG108-15 cells. (3/37)

1. In NG108-15 neuroblastomaxglioma hybrid cells, ATP stimulates intracellular cyclic AMP formation, which is inhibited by both adenosine (P(1)) and P2 receptor antagonists. In the present study, we examined the effects of several AMP derivatives in NG108-15 cells and mouse neuroblastoma N18TG-2 cells. 2. Adenosine 2'-monophosphate (A2P), adenosine 3'-monophosphate (A3P) and adenosine 5'-phosphosulphate (A5PS) increased cyclic AMP levels with similar concentration-dependencies in NG108-15 cells. 3. Increases in cyclic AMP by AMP derivatives were inhibited by the P2 receptor antagonist PPADS, but not by suramin. Effects of AMP derivatives were also inhibited by P(1) receptor antagonists ZM241385, XAC, DPCPX and partially by alloxazine. The ecto-nucleotidase inhibitor alpha, beta-methyleneADP was without effect. 4. In contrast, AMP derivatives did not change cyclic AMP levels in N18TG-2 cells. Accumulation of cyclic AMP in N18TG-2 cells was stimulated by adenosine A(2) receptor agonists CGS21680 and NECA, but not by ATP or beta, gamma-methyleneATP, agonists for cyclic AMP production in NG108-15 cells. 5. Reverse transcription-coupled polymerase chain reaction (RT - PCR) analyses revealed that N18TG-2 cells express both A(2A) and A(2B) receptors, while NG108-15 cells express mainly A(2A) receptors. 6. AMP derivatives did not affect the P2X and P2Y receptors expressed in NG108-15 cells. 7. These results suggest that A2P, A3P and A5PS act as agonists for cyclic AMP production and that these compounds are valuable tools for determinating the mechanism of ATP-stimulated cyclic AMP response in NG108-15 cells.  (+info)

Sulfate assimilation in higher plants characterization of a stable intermediate in the adenosine 5'-phosphosulfate reductase reaction. (4/37)

The enzyme catalysing the reduction of adenosine 5'-phosphosulfate (AdoPS) to sulfite in higher plants, AdoPS reductase, is considered to be the key enzyme of assimilatory sulfate reduction. In order to address its reaction mechanism, the APR2 isoform of this enzyme from Arabidopsis thaliana was overexpressed in Escherichia coli and purified to homogeneity. Incubation of the enzyme with [35S]AdoPS at 4 degrees C resulted in radioactive labelling of the protein. Analysis of APR2 tryptic peptides revealed 35SO2-3 bound to Cys248, the only Cys conserved between AdoPS and prokaryotic phosphoadenosine 5'-phosphosulfate reductases. Consistent with this result, radioactivity could be released from the protein by incubation with thiols, inorganic sulfide and sulfite. The intermediate remained stable, however, after incubation with sulfate, oxidized glutathione or AdoPS. Because truncated APR2, missing the thioredoxin-like C-terminal part, could be labelled even at 37 degrees C, and because this intermediate was more stable than the complete protein, we conclude that the thioredoxin-like domain was required to release the bound SO2-3 from the intermediate. Taken together, these results demonstrate for the first time the binding of 35SO2-3 from [35S]AdoPS to AdoPS reductase and its subsequent release, and thus contribute to our understanding of the molecular mechanism of AdoPS reduction in plants.  (+info)

Crystal structure of ATP sulfurylase from Saccharomyces cerevisiae, a key enzyme in sulfate activation. (5/37)

ATP sulfurylases (ATPSs) are ubiquitous enzymes that catalyse the primary step of intracellular sulfate activation: the reaction of inorganic sulfate with ATP to form adenosine-5'-phosphosulfate (APS) and pyrophosphate (PPi). With the crystal structure of ATPS from the yeast Saccharomyces cerevisiae, we have solved the first structure of a member of the ATP sulfurylase family. We have analysed the crystal structure of the native enzyme at 1.95 Angstroms resolution using multiple isomorphous replacement (MIR) and, subsequently, the ternary enzyme product complex with APS and PPi bound to the active site. The enzyme consists of six identical subunits arranged in two stacked rings in a D:3 symmetric assembly. Nucleotide binding causes significant conformational changes, which lead to a rigid body structural displacement of domains III and IV of the ATPS monomer. Despite having similar folds and active site design, examination of the active site of ATPS and comparison with known structures of related nucleotidylyl transferases reveal a novel ATP binding mode that is peculiar to ATP sulfurylases.  (+info)

Identification of yacE (coaE) as the structural gene for dephosphocoenzyme A kinase in Escherichia coli K-12. (6/37)

Dephosphocoenzyme A (dephospho-CoA) kinase catalyzes the final step in coenzyme A biosynthesis, the phosphorylation of the 3'-hydroxy group of the ribose sugar moiety. Wild-type dephospho-CoA kinase from Corynebacterium ammoniagenes was purified to homogeneity and subjected to N-terminal sequence analysis. A BLAST search identified a gene from Escherichia coli previously designated yacE encoding a highly homologous protein. Amplification of the gene and overexpression yielded recombinant dephospho-CoA kinase as a 22.6-kDa monomer. Enzyme assay and nuclear magnetic resonance analyses of the product demonstrated that the recombinant enzyme is indeed dephospho-CoA kinase. The activities with adenosine, AMP, and adenosine phosphosulfate were 4 to 8% of the activity with dephospho-CoA. Homologues of the E. coli dephospho-CoA kinase were identified in a diverse range of organisms.  (+info)

Assay of adenosine 3'-phosphate 5'-sulphatophosphate in hepatic tissues. (7/37)

A fluorimetric assay, based on the ability of boiled hepatic extracts to support the sulphO-conjugation of harmol, was used to demonstrate and quantify PAdoPS (adenosine 3'-phosphate 5'-sulphatophosphate) present in liver. A stoicheiometric relationship was established between the sulphate conjugate formed and the 'active sulphate' utilized. Guinea-pig, rat, mouse and rabbit livers contain 3.3, 2.9, 0.8 and 0.5 mumol of PAdoPS/100 G wet wt. respectively.  (+info)

The presence of an iron-sulfur cluster in adenosine 5'-phosphosulfate reductase separates organisms utilizing adenosine 5'-phosphosulfate and phosphoadenosine 5'-phosphosulfate for sulfate assimilation. (8/37)

It was generally accepted that plants, algae, and phototrophic bacteria use adenosine 5'-phosphosulfate (APS) for assimilatory sulfate reduction, whereas bacteria and fungi use phosphoadenosine 5'-phosphosulfate (PAPS). The corresponding enzymes, APS and PAPS reductase, share 25-30% identical amino acids. Phylogenetic analysis of APS and PAPS reductase amino acid sequences from different organisms, which were retrieved from the GenBank(TM), revealed two clusters. The first cluster comprised known PAPS reductases from enteric bacteria, cyanobacteria, and yeast. On the other hand, plant APS reductase sequences were clustered together with many bacterial ones, including those from Pseudomonas and Rhizobium. The gene for APS reductase cloned from the APS-reducing cyanobacterium Plectonema also clustered together with the plant sequences, confirming that the two classes of sequences represent PAPS and APS reductases, respectively. Compared with the PAPS reductase, all sequences of the APS reductase cluster contained two additional cysteine pairs homologous to the cysteine residues involved in binding an iron-sulfur cluster in plants. Mossbauer analysis revealed that the recombinant APS reductase from Pseudomonas aeruginosa contains a [4Fe-4S] cluster with the same characteristics as the plant enzyme. We conclude, therefore, that the presence of an iron-sulfur cluster determines the APS specificity of the sulfate-reducing enzymes and thus separates the APS- and PAPS-dependent assimilatory sulfate reduction pathways.  (+info)

Induction of neural differentiation in cultures of undetermined presumptive epidermis from three amphibian species was achieved by the addition of 1 millimolar dibutyryl adenosine 3,5-monophosphate, 8-bromadenosine 3,5-monophosphate, or adenosine C,E-monophosphate together with theophylline. Adenosine 5-monophosphate, adenosine 2,3-monophosphate, dibutyryl guanosine 3,5-monophosphate, and butyrate at 1 millimolar are ineffective. These results suggest that the action of the primary inductor or inductors may be mediated via adenosine 3,5-monophosphate. ...
Extracted from text ... MODAFINIL Modafinil has recently been approved by the South African Medicines Control Council (SAMCC) for the treatment of excessive daytime sleepiness (EDS) in patients with narcolepsy. It is registered as Provigil. The drug has also been used off-label for fatigue and sleepiness experienced by patients with multiple sclerosis (MS) and Parkinson's disease. Registered indication for modafinil: treatment of narcolepsy Modafinil is a unique wake-promoting agent that is chemically distinct from traditional stimulants. The precise mechanism of action in narcolepsy is not known, but it is believed to work selectively to activate the cortex of the brain. The drug is ..
TY - JOUR. T1 - Human 3′-Phosphoadenosine 5′-Phosphosulfate Synthetase (Isoform 1, Brain). T2 - Kinetic Properties of the Adenosine Triphosphate Sulfurylase and Adenosine 5′-Phosphosulfate Kinase Domains. AU - Lansdon, Eric B.. AU - Fisher, Andrew J. AU - Segel, Irwin H.. PY - 2004/4/13. Y1 - 2004/4/13. N2 - Recombinant human 3′-phosphoadenosine 5′-phosphosulfate (PAPS) synthetase, isoform 1 (brain), was purified to near-homogeneity from an Escherichia coli expression system and kinetically characterized. The native enzyme, a dimer with each 71 kDa subunit containing an adenosine triphosphate (ATP) sulfurylase and an adenosine 5′-phosphosulfate (APS) kinase domain, catalyzes the overall formation of PAPS from ATP and inorganic sulfate. The protein is active as isolated, but activity is enhanced by treatment with dithiothreitol. APS kinase activity displayed the characteristic substrate inhibition by APS (K1 of 47.9 μM at saturating MgATP). The maximum attainable activity of 0.12 ...
3-Phosphoadenosine-5-phosphosulfate. Key intermediate in the formation by living cells of sulfate esters of phenols, alcohols, steroids, sulfated polysaccharides, and simple esters, such as choline sulfate. It is formed from sulfate ion and ATP in a two-step process. This compound also is an important step in the process of sulfur fixation in plants and microorganisms ...
gi,23831324,sp,O43252.2,PAPS1_HUMAN RecName: Full=Bifunctional 3-phosphoadenosine 5-phosphosulfate synthase 1; Short=PAPS synthase 1; Short=PAPSS 1; AltName: Full=Sulfurylase kinase 1; Short=SK 1; Short=SK1; Includes: RecName: Full=Sulfate adenylyltransferase; AltName: Full=ATP-sulfurylase; AltName: Full=Sulfate adenylate transferase; Short=SAT; Includes: RecName: Full=Adenylyl-sulfate kinase; AltName: Full=3-phosphoadenosine-5-phosphosulfate synthase; AltName: Full=APS kinase; AltName: Full=Adenosine-5-phosphosulfate 3-phosphotransferase; AltName: Full=Adenylylsulfate 3- ...
Plants need to strike a fine balance between the production, transport, and degradation of stress signals in order to optimize growth in fluctuating environmental conditions. The signal 3′-phosphoadenosine 5′-phosphate (PAP) accumulates during drought and light stress and induces stress-responsive gene expression (Estavillo et al., 2011). Sulfur transfer from 3′-phosphoadenosine 5′-phosphosulfate (PAPS) to acceptor compounds in the cytosol results in the release of PAP. PAP is then degraded by the enzyme SAL1 in plastids and mitochondria into AMP and inorganic phosphate. SAL1 tightly controls PAP levels, as constitutively elevated PAP levels markedly affect growth and development (Estavillo et al., 2011). However, insights into how this signaling molecule moves between different cellular compartments, and the specific implications of subcellular concentrations, have been elusive. Ashykhmina et al. (2019) expand on the discovery of the plastid-specific PAPS transporter PAPST1 ...
SWISS-MODEL Repository entry for A0A5B7PAV2 (A0A5B7PAV2_ECOLX), Phosphoadenosine phosphosulfate reductase. Escherichia coli O26:H11
The sulphate activation and tyrosyl-protein sulphotransferase systems in normal 3Y1 rat embryo fibroblasts and the same cells transformed by Schmidt Ruppin subgroup-A-Rous sarcoma virus (SRA-3Y1) were examined. Employing metabolic [35S]sulphate-labelling followed by PEI (polyethyleneimine)-cellulose thin-layer chromatography of the labelled cell lysates, it was found that the steady-state level of active sulphate, adenosine 3′-phosphate 5′-phosphosulphate, was drastically lower in SRA-3Y1 cells compared with their normal counterparts. When the sulphate activating enzymes were tested, it appeared that the activities in 3Y1 homogenates were 2-2.5 times greater than those in SRA-3Y1 homogenates. An endogenous sulphation assay for tyrosyl-protein sulphotransferase revealed that activities in 3Y1 and SRA-3Y1 homogenates were comparable. Nearly identical patterns were observed with both sets of cells when [35S]sulphated proteins generated in the endogenous assay were separated by two-dimensional ...
Complete information for PAPSS2 gene (Protein Coding), 3-Phosphoadenosine 5-Phosphosulfate Synthase 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Horses of extreme and opposite morphological types:(a) Rapid Heavy Draft (brachymorphic type or heavy) and (b) Italian Trotter (dolichomorphic type or light).
MicroRNAs play a key role in the control of plant development and response to adverse environmental conditions. For example, microRNA395 (miR395), which targets three out of four isoforms of ATP sulfurylase, the first enzyme of sulfate assimilation, as well as a low-affinity sulfate transporter, SULTR2;1, is strongly induced by sulfate deficiency. However, other components of sulfate assimilation are induced by sulfate starvation, so that the role of miR395 is counterintuitive. Here, we describe the regulation of miR395 and its targets by sulfate starvation. We show that miR395 is important for the increased translocation of sulfate to the shoots during sulfate starvation. MiR395 together with the SULFUR LIMITATION 1 transcription factor maintain optimal levels of ATP sulfurylase transcripts to enable increased flux through the sulfate assimilation pathway in sulfate-deficient plants. Reduced expression of ATP sulfurylase (ATPS) alone affects both sulfate translocation and flux, but SULTR2;1 is ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Sulfation is a common modification of endogenous (lipids, proteins, and carbohydrates) and exogenous (xenobiotics and drugs) compounds. In mammals, the sulfate source is 3'-phosphoadenosine 5'-phosphosulfate (PAPS), created from ATP and inorganic sulfate. Two different tissue isoforms encoded by different genes synthesize PAPS. This gene encodes one of the two PAPS synthetases. Defects in this gene cause the Pakistani type of spondyloepimetaphyseal dysplasia. Two alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008 ...
1JEC: The complex structures of ATP sulfurylase with thiosulfate, ADP and chlorate reveal new insights in inhibitory effects and the catalytic cycle.
Catalysis of the transfer of an adenylyl group from an adenosine nucleotide (ATP or ADP) to sulfate, forming adenylylsulfate. [GOC:mah, MetaCyc:SULFATE-ADENYLYLTRANS-RXN, MetaCyc:SULFATE-ADENYLYLTRANSFERASE-ADP-RXN]
Papss2 - mouse gene knockout kit via CRISPR, 1 kit. |dl||dt|Kit Component:|/dt||dd|- |strong|KN312800G1|/strong|, Papss2 gRNA vector 1 in |a href=http://www.origene.com/CRISPR-CAS9/Detail.
1JED: The complex structures of ATP sulfurylase with thiosulfate, ADP and chlorate reveal new insights in inhibitory effects and the catalytic cycle.
Jonathan R. Widawsky , Masha Kamenetska , Young S. Park , Jennifer E. Klare , Colin Nuckolls , Michael L. Steigerwald , Mark S. Hybertson , Latha Venkataraman ...
Yersinia pseudotuberculosis serotype O:1b Phosphoadenosine phosphosulfate reductase (cysH) datasheet and description hight quality product and Backed by our Guarantee
Achondrogenesis is a number of disorders that are the most severe form of congenital chondrodysplasia (malformation of bones and cartilage). These conditions are characterized by a small body, short limbs, and other skeletal abnormalities. As a result of their serious health problems, infants with achondrogenesis are usually born prematurely, are stillborn, or die shortly after birth from respiratory failure. Some infants, however, have lived for a while with intensive medical support.. Researchers have described at least three forms of achondrogenesis, designated as Achondrogenesis type 1A, achondrogenesis type 1B and achondrogenesis type 2. These types are distinguished by their signs and symptoms, inheritance pattern, and genetic cause. Other types of achondrogenesis may exist, but they have not been characterized or their cause is unknown.. Achondrogenesis type 1A is caused by a defect in the microtubules of the Golgi apparatus. In mice, a nonsense mutation in the thyroid hormone receptor ...
Sulfur Assimilation and Abiotic Stress in Plants und Buchbewertungen gibt es auf ReadRate.com. Bücher können hier direkt online erworben werden.
Achondrogenesis refers to a group of rare and extreme skeletal dysplasias. Epidemiology The estimated incidence is 1:40,000 with no recognised gender predilection. Pathology It is classified as an osteochondrodysplasia, meaning deficiency of ...
Buy our Recombinant |em|E. coli |/em| cysH protein. Ab106888 is a full length protein produced in Escherichia coli and has been validated in SDS-PAGE, MS…
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Y. Yin , J. Chudow , D.F. Santavicca , V. Manucharyan , A.J. Annunziata , L. Frunzio , D.E. Prober , B. Reulet , A. True , C.A. Schmuttenmaer , M. Purewal , Y. Zuev , P. Kim ...
Achondrogenesis is a severe skeletal dysplasia that is invariably lethal before or after birth. Achondrogenesis type IA (ACG1A; MIM 200600) and IB (ACG1B; MIM 600972) are autosomal recessive disorders caused by mutations in the TRIP11 and SLC26A2 genes, respectively. Achondrogenesis type II (ACG2; MIM 200610) is an autosomal dominant disorder caused by mutations in the COL2A1 gene. All types are clinically overlapping and are mainly characterized by short-limbed dwarfism, short ribs, small chest, absent or minimally ossified vertebral bodies, and hydrops fetalis.. Copy number variation (CNV) analysis of the Achondrogenesis genes is also offered as a panel. Additionally, CTGT offers a comprehensive test (both NGS and CNV panels) for these genes. Panel genes are also offered as individual sequencing and deletion/duplication tests unless otherwise indicated.. Read less ...
Background: Achondrogenesis type II is a lethal form of osteochondrodysplasia characterized by short trunk, disproportionately large head, prominent forehead, micrognathia, extreme micromelia, anasarca, large abdomen and poor ossification of the bones. The children with achondrogenesis are usually born premature, or die in the neonatal period mostly from respiratory failure. We report the case of a live term newborn infant with achondrogenesis type II who died shortly after birth. Methods: We report a case of achondrogenesis type II in a live male newborn. Results: We report the case of a term male infant delivered to a 24-year-old woman with a chondrogenesis type II confirmed radiologically but died at age 5 days. Conclusion: Whenever a skeletal dysplasia in a fetal dwarfism is suspected, a proper work-up plan should be done to evaluate family history. A clinical, radiographic and histopathologic examination, should be done and confirmed by genetic study. Following evidence-based diagnosis, patients
0063] In one embodiment, the primer extension products for the rare mutations are detected using PYROSEQUENCING® (Uppsala, Sweden), which is essentially sequencing by synthesis. A sequencing primer, designed to anneal directly next to the nucleic acid differing between the rare and the common allele or the artificially produced quantification standard is first hybridized to a single stranded, PCR amplified, DNA template comprising both the target and the standard PCT product, and incubated with the enzymes, DNA polymerase, ATP sulfurylase, luciferase and apyrase, and the substrates, adenosine 5 phosphosulfate (APS) and luciferin. One of four deoxynucleotide triphosphates (dNTP), for example, corresponding to the nucleotide present in the standard template, is then added to the reaction. DNA polymerase catalyzes the incorporation of the dNTP into the standard DNA strand. Each incorporation event is accompanied by release of pyrophosphate (PPi) in a quantity equimolar to the amount of ...
This family consists of phosphodiesterases, including human plasma-cell membrane glycoprotein PC-1 / alkaline phosphodiesterase I / nucleotide pyrophosphatase (nppase). These enzymes catalyse the cleavage of phosphodiester and phosphosulphate bonds in NAD, deoxynucleotides and nucleotide sugars [(PUBMED:9344668)]. Another member of this family is ATX an autotaxin, tumor cell motility-stimulating protein which exhibits type I phosphodiesterases activity [(PUBMED:7982964)]. The alignment encompasses the active site [(PUBMED:7730366), (PUBMED:7982964)]. Also present within this family is 60 kDa Ca2+-ATPase from Myroides odoratus [(PUBMED:8617788)].. This signature also hits a number of ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis.. ...
The Entamoeba transmembrane mitosomal protein of 30 kDa (ETMP30) of 260 aas and 5 equally distantly spaced TMSs. Its loss results in a defect in growth and partial elongation of mitosomes (Santos et al. 2019). The aerobic mitochondrion had undergone evolutionary diversification, most notable among lineages of anaerobic protists. Entamoeba is one of the genera of parasitic protozoans that lack canonical mitochondria, and instead possess mitochondrion-related organelles (MROs), specifically, mitosomes. Entamoeba mitosomes exhibit functional reduction and divergence, most exemplified by the organelles inability to produce ATP and synthesize iron-sulfur clusters. Instead, this organelle is capable of sulfate activation, which has been linked to amoebic stage conversion (Santos et al. 2019). Colocalization of hemagglutinin (HA)-tagged ETMP30 with the mitosomal marker, adenosine-5-phosphosulfate kinase. Mitosomal membrane localization was indicated by immunoelectron microscopy analysis. ...
Achondrogenesis, type IB (ACG1B; MIM 600972) is a lethal condition caused by the diastrophic dysplasia sulfate transporter gene mutations (DTDST/SLC26A2). Affected fetuses are usually in breech position and appear hydropic. The limbs and the trunk are shortened. In contrast to the findings in achondrogenesis, type II (ACG2; MIM 200610), the head is of a normal or near normal size and the fingers and toes are short. The feet and toes are rotated inward, a finding shared with diastrophic dysplasia (DTD; MIM 222600), another disorder caused by DTDST mutations.. Read less ...
Previous studies have indicated that transforming growth factor β (TGF-β) signaling has a critical role in cartilage homeostasis and repair, yet the mechanisms of TGF-βs chondroprotective effects are not known. Our objective in this study was to identify downstream targets of TGF-β that could act to maintain biochemical and biomechanical properties of cartilage. Tibial joints from 20-week-old mice that express a dominant-negative mutation of the TGF-β type II receptor (DNIIR) were graded histologically for osteoarthritic changes and tested by indentation to evaluate their mechanical properties. To identify gene targets of TGF-β, microarray analysis was performed using bovine articular chondrocytes grown in micromass culture that were either treated with TGF-β or left untreated. Phosphoadenosine phosphosynthetase 2 (PAPSS2) was identified as a TGF-β-responsive gene. Papss2 expression is crucial for proper sulfation of cartilage matrix, and its deficiency causes skeletal defects in mice and
Grigelioniene G, Geiberger S, Papadogiannakis N, Mäkitie O, Nishimura G, Nordgren A, Conner P. 2013. The phenotype range of achondrogenesis 1A. Am J Med Genet Part A 161A:2554-2558 ...
LOL. Again the other thread was more about getting boys snipped than men. A few people pointed out that there children who were not snipped had had really bad health problems, and snipping seemed to fix this. Others mentioned that it slowed the spread of HPV Human pap virus. I was doing some research to confirm this and came across the HIV news. Since its a pretty deadly disease and this is something that men can do to cut their risk by 50% if they are not using condoms, and even if they are, just cut down the risk that much further ...
APS Industrial Building is a Commercial Building located in Bishan. A number of Business, Electric Connector and others 51 business can be found in APS Industrial Building.
USAlive! (Alive) is a Belgian Strong Pale Ale style beer brewed by Mikkeller ApS in København, Denmark. 3.92 average with 205 ratings, reviews and opinions.
Search PubMed for more publications by David Eide. Qiao, W., Ellis, C., Steffen, J., Wu, C., and Eide, D. Zinc status and vacuolar zinc transporters control alkaline phosphatase accumulation and activity in Saccharomyces cerevisiae. Mol. Microbiol. 72:320-34 (2009).. Wu, C., Roje, S., Sandoval, F., Bird, A., Winge, D., and Eide, D. Repression of sulfate assimilation is an adaptive response of yeast to the oxidative stress of zinc deficiency. J. Biol. Chem. 284:27544-56 (2009).. Wu, C., Steffen, J., and Eide, D. Cytosolic superoxide dismutase (SOD1) is critical for tolerating the oxidative stress of zinc deficiency in yeast. PLoS One 16;4(9):e7061(2009).. Eide, D.J. Homeostatic and adaptive responses to zinc deficiency in Saccharomyces cerevisiae. J. Biol. Chem. 284:18565-18569 (2009).. Atkinson, A., Khalimonchuk, O., Smith, P., Sabic, H., Eide, D., and Winge, D. Mzm1 influences a labile pool of mitochondrial zinc important for respiratory function. J. Biol. Chem. 285:19450-19459 (2010).. Frey, ...
Adenylylsulphate (adenosine-5′-phosphosulphate, APS) reductase from the extremely thermophilic sulphate-reducing archaeon Archaeoglobus fulgidus is an iron-sulphur flavoprotein containing one non-covalently bound flavin group, eight non-haem iron and six labile sulphide atoms per molecule. Re-evaluation of the enzyme structure revealed the presence of two different subunits with molecular masses of 80 and 18.5 kDa. The subunits are arranged in an α2β subunit structure. We have cloned and sequenced a 2.7 kb segment of DNA containing the genes for the α and β subunits, which we designate aprA and aprB, respectively. The two genes are separated by 17 bp and localized in the order aprBA. While a putative promoter could not be identified in the vicinity of aprBA a probable termination signal was found just downstream of the translation stop codon of aprA. The codon usage for aprBA shows strong preferences for G and C in the third codon position. aprA encodes a 73.3 kDa polypeptide, which shows
TY - JOUR. T1 - The human estrogen sulfotransferase. T2 - A half-site reactive enzyme. AU - Sun, Meihao. AU - Leyh, Thomas S.. N1 - Copyright: Copyright 2010 Elsevier B.V., All rights reserved.. PY - 2010/6/15. Y1 - 2010/6/15. N2 - The affinity of 17β-estradiol (E2) for the estrogen receptor is weakened beyond the point of physiological relevance by the transfer of the sulfuryl moiety (-SO3) from PAPS (3′-phosphoadenosine 5′-phosphosulfate) to the 3′-hydroxyl of E2. The mechanism of this transfer reaction, catalyzed by estrogen sulfotransferase (EST), is investigated here in detail. The enzyme (a dimer of identical protomers) presents a clear example of half-sites reactivityonly one of the subunits of the dimer produces product during the catalytic cycle. This is the first example of half-sites reactivity in the sulfotransferase family. A burst of product, with an amplitude that corresponds to one-half of the available active sites, reveals that the mechanism is rate-limited by product ...
TY - JOUR. T1 - Zebrafish as a model for the study of the phase II cytosolic sulfotransferases. AU - Liu, Tzu An. AU - Bhuiyan, Shakhawat. AU - Liu, Ming Yih. AU - Sugahara, Takuya. AU - Sakakibara, Yoichi. AU - Suiko, Masahito. AU - Yasuda, Shin. AU - Kakuta, Yoshimitsu. AU - Kimura, Makoto. AU - Williams, Frederick E.. AU - Liu, Ming Cheh. PY - 2010/7/1. Y1 - 2010/7/1. N2 - Cytosolic sulfotransferases (SULTs) are traditionally known as the Phase II drug-metabolizing or detoxifying enzymes that serve for the detoxification of drugs and other xenobiotics. These enzymes in general catalyze the transfer of a sulfonate group from the active sulfate, 3′-phophoadenosine 5′-phosphosulfate (PAPS), to low-molecular weight substrate compounds containing hydroxyl or amino group(s). Despite considerable efforts made in recent years, some fundamental aspects of the SULTs, particularly their ontogeny, cell type/tissue/organ-specific distribution, and physiological relevance, particularly their ...
glycolithocholate sulfate = N-(3α-sulfooxy-5β-cholan-24-oyl)glycine. Other name(s): BAST I; bile acid:3-phosphoadenosine-5-phosphosulfate sulfotransferase; bile salt:3phosphoadenosine-5-phosphosulfate:sulfotransferase; bile acid sulfotransferase I; glycolithocholate sulfotransferase. Systematic name: 3-phosphoadenylyl-sulfate:glycolithocholate sulfotransferase. Comments: The formation of sulfate esters of bile acids is an essential step in the prevention of toxicity by monohydroxy bile acids in many species [3]. This enzyme is both a bile salt and a 3-hydroxysteroid sulfotransferase. In addition to the 5β-bile acid glycolithocholate, deoxycholate, 3β-hydroxy-5-cholenoate and dehydroepiandrosterone (3β-hydroxyandrost-5-en-17-one) also act as substrates [see also EC 2.8.2.2 (alcohol sulfotransferase) and EC 2.8.2.34 (glycochenodeoxycholate sulfotransferase)]. May be identical to EC 2.8.2.2 [3].. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: ...
Autor: Krueger, S. et al.; Genre: Zeitschriftenartikel; Im Druck veröffentlicht: 2009; Keywords: non-aqueous fractionation|br/|organelles|br/|regulation|br/|subcellular|br/|sulphur metabolism|br/|acetylserine thiol lyase|br/|synthase protein complex|br/|amino-acids|br/|gene family|br/|spinach leaves|br/|potato-tubers|br/|higher-plants|br/|salmonella-typhimurium|br/|sulfate assimilation|br/|hydrogen-sulfide; Titel: Analysis of cytosolic and plastidic serine acetyltransferase mutants and subcellular metabolite distributions suggests interplay of the cellular compartments for cysteine biosynthesis in Arabidopsis
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2 detailed and in-depth reviews for Chaind Reaction: ----Introduction---- APS is one of my favorite brands. I have tried lots of aminos with different qualities and effectiveness. I dont think they are a necessity but they are nice to have in my opinion and this is probably the best one Ive had. ----Ingredient Profile---- 2 scoops (12g) The ratio
Abstract Sulphur is an essential element that all pathogens have to absorb from their surroundings in order to grow inside their infected host. Despite its importance, the relevance of sulphur assimilation in fungal virulence is largely unexplored. Here we report a role of the bZIP transcription factor MetR in sulphur assimilation and virulence of the human pathogen Aspergillus fumigatus. The MetR regulator is essential for growth on a variety of sulphur sources; remarkably, it is fundamental for assimilation of inorganic S-sources but dispensable for utilization of methionine. Accordingly, it strongly supports expression of genes directly related to inorganic sulphur assimilation but not of genes connected to methionine metabolism. On a broader scale, MetR orchestrates the comprehensive transcriptional adaptation to sulphur-starving conditions as demonstrated by digital gene expression analysis. Surprisingly, A. fumigatus is able to utilize volatile sulphur compounds produced by its methionine ...
Sulfation or sulfurylation (not to be confused with sulfonation) in biochemistry is the enzyme-catalyzed conjugation of a sulfo group (not a sulfate or sulfuryl group) to another molecule. This biotransformation involves a sulfotransferase enzyme catalyzing the transfer of a sulfo group from a donor cosubstrate, usually 3-phosphoadenosine-5-phosphosulfate (PAPS), to a substrate molecules hydroxyl or amine. Sulfation is involved in a variety of biological processes, including detoxification, hormone regulation, molecular recognition, cell signaling, and viral entry into cells. It is among the reactions in phase II drug metabolism, frequently effective in rendering a xenobiotic less active from a pharmacological and toxicological standpoint, but sometimes playing a role in the activation of xenobiotics (e.g. aromatic amines, methyl-substituted polycyclic aromatic hydrocarbons). Another example of biological sulfation is in the synthesis of sulfonated glycosaminoglycans, such as heparin, heparan ...
TY - JOUR. T1 - Phenolsulphotransferase in human tissue. T2 - Radiochemical enzymatic assay and biochemical properties. AU - Anderson, Robert J.. AU - Weinshilboum, R. M.. PY - 1980/4/11. Y1 - 1980/4/11. N2 - Phenolsulphotransferase (EC 2.8.2.1) (PST) is an important catecholamine and drug metabolizing enzyme. Optimal conditions have been determined for the accurate measurement of PST activity in the human platelet, human renal cortex, and human jejunum with a radiochemical microassay. 3-Methoxy-4-hydroxyphenylglycol (MHPG) and 35S-3-phosphoadenosine-5-phosphosulfate (35S-PAPS) were the substrates for the reaction. The apparent Michaelis-Menten (Km) values for MHPG with platelet, renal cortex, and jejunum were 1.09, 0.46 and 1.16 mmol/l, respectively. Apparent Km values for PAPS in the same tissues were 0.14, 0.13 and 0.21 μmol/l. The pH optimum of the reaction in all three tissues was approximately 6.2-6.8 with three different buffer systems. The coefficients of variation for the assay of ...
Jez JM, Lee SG, Sherp AM (2016) The next green movement: plant biology for the environment and sustainability. Science 353, 1241-4. Kilgore M, Holland CK, Jez JM, Kutchan TM (2016) Identification of a noroxomaritidine reductase with Amaryllidaceae alkaloid biosynthesis-related activities. J Biol Chem 291, 16740-52. Lee SG, Nwumeh R, Jez JM (2016) Structure and mechanism of isopropylmalate dehydrogenase from Arabidopsis thaliana: insights on leucine and aliphatic glucosinolate biosynthesis. J Biol Chem 291, 13421-30. Herrmann J, Nathin D, Lee SG, Sun T, Jez JM (2015) Recapitulating the structural evolution of redox regulation in adenosine-5-phosphosulfate kinase from cyanobacteria to plants. J Biol Chem 290, 24705-14. Korasick DA, Jez JM, Strader LC (2015) Refining the nuclear auxin response pathway through structural biology. Curr Opin Plant Biol 27, 22-8. Cahoon RE, Lutke WK, Cameron JC, Chen S, Lee SG, Rivard RS, Rea PA, Jez JM (2015) Adaptive engineering of phytochelatin-based heavy metal ...
Major isoenzymes of androsterone-sulphating sulphotransferase (AD-ST) were isolated from liver cytosols of weanling and young adult female rats and their isoelectric properties were compared. On chromatofocusing the enzyme activity of young adults was eluted over a wider range of pH than was that of weanling rats. The activity at pH 7.8-7.2 (fraction I) is obvious at both ages, whereas the activity eluted over the pH 6.6-5.5 range (fraction II) is much lower in weanlings than in young adults. The AD-ST activities eluted in fractions I and II were separately purified by 3′-phosphoadenosine 5′-phosphate-agarose affinity chromatography at both ages. Two-dimensional gel electrophoresis of the isolated enzyme revealed several subunits with distinct pI values, but with the same molecular mass, namely 30 kDa. The relative levels of the pI 6.7 and pI 7.2 subunits are high and the relative level of the pI 6.1 is low in fraction I. In fraction II, the levels of pI 6.1 and pI 6.7 subunits are high and ...
The adrenal steroid dehydroepiandrosterone (DHEA) and its sulphate ester, DHEAS are the most abundant circulating steroid hormones in humans. Uncongugated DHEA predominately exerts its effects via its downstream conversion to active sex steroids in peripheral target tissues. In contrast the conversion of DHEAS to androgens first requires cleavage of the sulfate group, catalysed by the microsomal enzyme steroid sulfatase (STS). Conversely, DHEA is converted to inactive DHEAS by the activity of the cytosolic enzyme DHEA sulphotransferase (SULT2A1). However, in addition, evidence is growing that DHEA and DHEAS can have specific, direct effects. In this thesis, I have demonstrated that abrogation of DHEA metabolism can result in the manifestation of pathophysiological conditions. SULT2A1 requires 3-phosphoadenosine-5-phosphosulfate (PAPS) for catalytic activity. I have identified compound heterozygous mutations in the gene encoding human PAPS synthase 2 (PAPSS2) in a girl with androgen excess and ...
However, in multiplex Pyrosequencing, more proportional signals are expected in a Addition of this enzyme has eliminated the need for solid support and interme- diate washing thereby enabling the pyrosequencing re- action to be performed in a single tube. a real-time DNA sequencing technique based on monitoring DNA synthesis by BIOLUMINESCENCE using four enzymes: DNA POLYMERASE, ATP sulfurylase, LUCIFERASE and apyrase. Application of Pyrosequencing Pyrosequencing has shown well performance in determination of complex DNA structure such as cDNA analysis, mutation detection Re-sequencing of diseases linked genes, viral typing, bacterial typing, and SNPs. Detection ( light sensor) V. Washing or add enzyme apyrase enzyme. Das Verfahren wird von vier verschiedenen Enzymen eingesetzt: DNA-Polymerse, ATP-Sulfurylase, Luciferase und Apyrase und zwei Substrate Adenosin-5-phosphosulfat (APS) und Luciferin. [12,13] Unused dNTPs are washed out with the apyrase. Pyrosequencing, a nonelectrophoretic DNA ...
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... phosphosulfate kinase, adenosine phosphosulfate kinase, adenosine phosphosulfokinase, adenosine-5'-phosphosulfate-3'- ... MacRae IJ, Rose AB, Segel IH (October 1998). "Adenosine 5'-phosphosulfate kinase from Penicillium chrysogenum. site-directed ... Other names in common use include adenylylsulfate kinase (phosphorylating), 5'-phosphoadenosine sulfate kinase, adenosine 5'- ... phosphosulfate synthase and its functional domains". J. Biol. Chem. 273 (30): 19311-20. doi:10.1074/jbc.273.30.19311. PMID ...
PPi is pyrophosphate APS is adenosine 5-phosphosulfate; ATP is adenosine triphosphate; O2 is oxygen molecule; AMP is adenosine ... and with the substrates adenosinephosphosulfate (APS) and luciferin. The addition of one of the four deoxynucleotide ... This incorporation releases pyrophosphate (PPi). ATP sulfurylase converts PPi to ATP in the presence of adenosine 5´ ... phosphosulfate. This ATP acts as a substrate for the luciferase-mediated conversion of luciferin to oxyluciferin that generates ...
... phosphosulfate-forming). Other names in common use include adenosine phosphosulfate reductase, adenosine 5'-phosphosulfate ... Grant K, Carey NM, Mendoza M, Schulze J, Pilon M, Pilon-Smits EA, van Hoewyk D (September 2011). "Adenosine 5'-phosphosulfate ... 2018). "Central Role of Adenosine 5'-Phosphosulfate Reductase in the Control of Plant Hydrogen Sulfide Metabolism". Frontiers ... phosphosulfate reductase. Adenosine 5'-monophosphate inhibitor and key role of arginine 317 in switching the course of ...
Other names in common use include adenosine 5-phosphosulfate sulfohydrolase, and adenylylsulfate sulfohydrolase. This enzyme ...
... adenosine 5'phosphosulfate sulfohydrolase and adenosine-5'-phosphosulfate cyclase activities". Eur. J. Biochem. 65 (1): 113-21 ... adenosine 5'-phosphate + phosphate Thus, the two substrates of this enzyme are adenosine 3',5'-bisphosphate and H2O, whereas ... In enzymology, a 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) is an enzyme that catalyzes the chemical reaction adenosine 3 ... The systematic name of this enzyme class is adenosine-3'(2'),5'-bisphosphate 3'(2')-phosphohydrolase. Other names in common use ...
Abola AP, Willits MG, Wang RC, Long SR (1999). "Reduction of Adenosine-5′-Phosphosulfate Instead of 3′-Phosphoadenosine-5′- ... The systematic name of this enzyme class is AMP, sulfite:thioredoxin-disulfide oxidoreductase (adenosine-5'-phosphosulfate- ... Williams SJ, Senaratne RH, Mougous JD, Riley LW, Bertozzi CR (2002). "5'-adenosinephosphosulfate lies at a metabolic branch ... Phosphosulfate in Cysteine Biosynthesis by Rhizobium meliloti and Other Members of the Family Rhizobiaceae". J. Bacteriol. 181 ...
Li, Jiayang (1991). Purification and properties of ATP sulfurylase and adenosine 5'-phosphosulfate sulfotransferase from ... ATP sulfurylase and adenosine 5'-phosphosulfate sulfotransferase) in the single-celled organism Euglena. Following his PhD, Li ... CS1 maint: discouraged parameter (link) Li, J. Y.; Schiff, J. A. (1991). "Purification and properties of adenosine 5'- ... phosphosulphate sulphotransferase from Euglena". The Biochemical Journal. 274 (2): 355-60. doi:10.1042/bj2740355. PMC 1150144. ...
The enzymes reduce adenosine-5'-phosphosulfate by nucleophilic attack to produce the sulfite product. This typically involves a ...
17 Reactions of the adenosine 5'-phosphosulfate (APS) sulfotransferase from Chlorella and studies of model reactions which ... Schmidt A (January 1976). "The adenosine-5'-phosphosulfate sulfotransferase from spinach (Spinacea oleracea L.). Stabilization ... Other names in common use include phosphoadenylylsulfate-thiol sulfotransferase, PAPS sulfotransferase, and adenosine 3'- ... adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate Thus, the two substrates of this enzyme are 3'-phosphoadenylyl sulfate ...
Sulfate needs to be activated to adenosine 5'-phosphosulfate (APS) prior to its reduction to sulfite. The activation of sulfate ...
The systematic name of this enzyme class is AMP,sulfite:glutathione-disulfide oxidoreductase (adenosine-5'-phosphosulfate- ... adenosine-5'-phosphosulfate-forming), and plant-type 5'-adenylylsulfate reductase. In plants, APS is reduced by the plastidic ... 2 glutathione The 3 substrates of this enzyme are adenosine monophosphate, sulfite, and glutathione disulfide, whereas its two ...
... kinetic properties of the adenosine triphosphate sulfurylase and adenosine 5'-phosphosulfate kinase domains". Biochemistry. 43 ... Sekulic N, Konrad M, Lavie A (2007). "Structural mechanism for substrate inhibition of the adenosine 5'-phosphosulfate kinase ... 1998). "cDNA cloning, expression, and characterization of the human bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate ... In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 ...
"Genomic organization of the mouse and human genes encoding the ATP sulfurylase/adenosine 5'-phosphosulfate kinase isoform SK2 ... "Entrez Gene: PAPSS2 3'-phosphoadenosine 5'-phosphosulfate synthase 2". Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale ... Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthetase 2 is an enzyme that in humans is encoded by the PAPSS2 gene. ... In mammals, the sulfate source is 3'-phosphoadenosine 5'-phosphosulfate (PAPS), created from ATP and inorganic sulfate. Two ...
This is done by the enzyme ATP-sulfurylase, which uses ATP and sulfate to create adenosine 5'-phosphosulfate (APS). APS is ...
... usually adenosine 5'-phosphosulfate (APS) or 3'-phosphoadenosine-5'-phosphosulfate (PAPS). Sulfate esters may be hydrolyzed by ...
In this biochemical pathway, AMP reacts with sulfite in the presence of the enzyme APS reductase, giving APS (adenosine 5'- ... phosphosulfate). Then, APS reacts with the enzyme ATP sulfurylase in presence of pyrophosphate (PPi) giving ATP (substrate- ...
... adenosine 3',5'-bisphosphate,sulfite:oxidized-thioredoxin, and oxidoreductase (3'-phosphoadenosine-5'-phosphosulfate-forming). ... adenosine 3'-phosphate 5'-phosphosulfate reductase, 3'-phosphoadenylylsulfate reductase, thioredoxin:3'-phospho-adenylylsulfate ... The systematic name of this enzyme class is adenosine 3',5'-bisphosphate,sulfite:thioredoxin-disulfide oxidoreductase (3'- ... phosphoadenosine-5'-phosphosulfate-forming). Other names in common use include PAPS reductase, thioredoxin-dependent, PAPS ...
Both enzymes are required for PAPS (phosphoadenosine-phosphosulfate) synthesis from inorganic sulfate. As of late 2007, 18 ... Other names in common use include adenosine-5'-triphosphate sulfurylase, adenosinetriphosphate sulfurylase, adenylylsulfate ... Some sulfate adenylyltransferases are part of a bifunctional polypeptide chain associated with adenosyl phosphosulfate (APS) ... phosphosulfate synthase and its functional domains". J. Biol. Chem. 273 (30): 19311-20. doi:10.1074/jbc.273.30.19311. PMID ...
ATP sulfurylase first generates adenosine-5'-phosphosulfate (APS) and then APS kinase transfers a phosphate from ATP to APS to ... Kamio, K.; Honke, K.; Makita, A. (Dec 1995). "Pyridoxal 5'-phosphate binds to a lysine residue in the adenosine 3'-phosphate 5 ... In eukaryotic cells the activated sulfate donor is 3'-phosphoadenosine-5'-phosphosulfate (PAPS) (Figure 1). PAPS is synthesized ... May 1997). "Sulfation and sulfotransferases 5: the importance of 3'-phosphoadenosine 5'-phosphosulfate (PAPS) in the regulation ...
It is predicted that the potential use of the sox genes would be in the adenosine 5'-phosphosulfate (APS)/ 3'-Phosphoadenosine- ... 5'-phosphosulfate (PAPS) pathway. Though KT71 and other AAnPs can make up over 10% of the bacterioplankonic community in ...
The reduction of sulfate in nature involves the formation of one or sometimes two sulfate esters, adenosine 5'-phosphosulfate ( ... APS) and 3'-phosphoadenosine-5'-phosphosulfate (PAPS). Sulfate is an inert anion, so nature activates it by the formation of ...
... adenosine 5'-phosphosulfate) thereby consuming ATP. The APS is then reduced by the enzyme APS reductase to form sulfite (SO2− 3 ...
Heparan sulfate 6-sulfotransferases catalyses the transfer of sulfate from adenosine 3'-phosphate, 5'-phosphosulfate to the 6th ...
... phosphosulfate and 5beta-scymnol, whereas its two products are adenosine 3',5'-bisphosphate and 5beta-scymnol sulfate. This ... phosphosulfate + 5beta-scymnol ⇌ {\displaystyle \rightleftharpoons } adenosine 3',5'-bisphosphate + 5beta-scymnol sulfate Thus ... The systematic name of this enzyme class is 3'-Phosphoadenosine-5'-phosphosulfate:5beta-scymnol sulfotransferase. Macrides TA, ...
... occurs in four steps: Conversion (activation) of sulfate to Adenosine 5'-phosphosulfate (APS) ...
... phosphosulfate + chondroitin ⇌ {\displaystyle \rightleftharpoons } adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate Thus, ... I Transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to mucopolysaccharides". J. Biol. Chem. 235: 257-266. PMID ... the two substrates of this enzyme are 3'-phosphoadenylyl sulfate and chondroitin, whereas its two products are adenosine 3',5'- ...
... and adenosine 5'-phosphosulfate (APS), which are known to have a significant oxygen isotope exchange. The step catalyzed by SQR ... which is dependent on adenosine monophosphate (AMP), and oxidative phosphorylation independent of AMP, which has been detected ...
... known as Aps until 1904 Adenosine 5'-phosphosulfate, a metabolic precursor to 3'-phosphoadenosine-5'-phosphosulfate (PAPS) ...
... phosphosulfate. This product is then hydrolysed by 3'(2'),5'-bisphosphate nucleotidase to give adenosine monophosphate, which ... Adenosine 3',5'-bisphosphate is a form of an adenosine nucleotide with two phosphate groups attached to different carbons in ... Adenosine 3',5'-bisphosphate is produced as a product of sulfotransferase enzymes from the donation of a sulfate group from the ... This is distinct from adenosine diphosphate, where the two phosphate groups are attached in a chain to the 5' carbon atom in ...
3′-Phosphoadenosine-5′-phosphosulfate (PAPS) is a derivative of adenosine monophosphate that is phosphorylated at the 3′ ... It is endogenously synthesized by organisms via the phosphorylation of adenosine 5′-phosphosulfate (APS), an intermediary ... In humans such reaction is performed by bifunctional 3′-phosphoadenosine 5′-phosphosulfate synthases (PAPSS1 and PAPSS2) using ... Venkatachalam, K. V. (2003). "Human 3′-phosphoadenosine 5′-phosphosulfate (PAPS) synthase: Biochemistry, molecular biology and ...
The reduction of sulfate in nature involves the formation of one or sometimes two sulfate esters, adenosine 5'-phosphosulfate ( ... APS) and 3'-phosphoadenosine-5'-phosphosulfate (PAPS). Sulfate is an inert anion, so nature activates it by the formation of ...
Adenosine triphosphate [41]. Phosphate group. Bacteria, archaea and eukaryotes S-Adenosyl methionine [42]. Methyl group. ... 3'-Phosphoadenosine-5'-phosphosulfate [55]. Sulfate group. Bacteria, archaea and eukaryotes Pyrroloquinoline quinone [56]. ... Many contain the nucleotide adenosine monophosphate (AMP) as part of their structures, such as ATP, coenzyme A, FAD, and NAD+. ... Organic cofactors may have been present even earlier in the history of life on Earth.[65] The nucleotide adenosine is present ...
1996). "Purification and characterization of 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase from human renal ... a galactosylceramide to adenosine 3',5'-bisphosphate + galactosylceramide sulfate. Activity of this sulfotransferase is ... 1977). "Age-dependent modulation of 3'-phosphoadenosine-5'-phosphosulfate-galactosylceramide sulfotransferase by lipids ...
Harada T, Shimizu S, Nakanishi Y, Suzuki S (1967). "Enzymatic transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to ... adenosine 3',5'-bisphosphate + UDP-N-acetyl-D-galactosamine 4,6-bissulfate Thus, the two substrates of this enzyme are 3'- ... phosphoadenylyl sulfate and UDP-N-acetyl-D-galactosamine 4-sulfate, whereas its two products are adenosine 3',5'-bisphosphate ...
Other names in common use include 3-phosphoadenylyl sulfatase, 3-phosphoadenosine 5-phosphosulfate sulfatase, PAPS sulfatase, ... adenosine 3',5'-bisphosphate + sulfate Thus, the two substrates of this enzyme are 3'-phosphoadenylyl sulfate and H2O, whereas ... its two products are adenosine 3',5'-bisphosphate and sulfate. This enzyme belongs to the family of hydrolases, specifically ...
... phosphosulfate (PAPS) to phenolic compounds, resulting in more hydrophilic products that can be more easily expelled from ... which is a single nucleotide polymorphism at the 638th base of the SULT1A1 gene from guanine to adenosine that causes the ...
Other names in common use include GSase, 3'-phosphoadenosine-5'-phosphosulfate-cerebroside sulfotransferase, galactocerebroside ... adenosine 3',5'-bisphosphate + a galactosylceramidesulfate Thus, the two substrates of this enzyme are 3'-phosphoadenylyl ... sulfate and galactosylceramide, whereas its two products are adenosine 3',5'-bisphosphate and galactosylceramidesulfate. This ...
Other names in common use include PAPS:psychosine sulphotransferase, and 3'-phosphoadenosine 5'-phosphosulfate-psychosine ... adenosine 3',5'-bisphosphate + psychosine sulfate Thus, the two substrates of this enzyme are 3'-phosphoadenylyl sulfate and ... galactosylsphingosine, whereas its two products are adenosine 3',5'-bisphosphate and psychosine sulfate. This enzyme belongs to ...
Here, GalCer reacts with 3'-phosphoadenosine-5'-phosphosulfate (PAPS) to make sulfatide. This reaction is catalyzed by ... which sulfatide is capable of mediating through stimulation of calcium dependent exocytosis and adenosine triphosphate (ATP)- ...
Adenosine 3-phospho 5-phosphosulfate transporter 1 is a protein that in humans is encoded by the SLC35B2 gene. Solute carrier ... 2003). "Molecular cloning and identification of 3-phosphoadenosine 5-phosphosulfate transporter". J. Biol. Chem. 278 (28): ...
... phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl donor for sulfation events that take place in the ... Adenosine 3-phospho 5-phosphosulfate transporter 1Add BLAST. 431. Proteomic databases. PaxDb, a database of protein abundance ... sp,Q91ZN5,S35B2_MOUSE Adenosine 3-phospho 5-phosphosulfate transporter 1 OS=Mus musculus OX=10090 GN=Slc35b2 PE=1 SV=1 ... Mediates the transport of adenosine 3-phospho 5-phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl ...
... phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl donor for sulfation events that take place in the ... Adenosine 3-phospho 5-phosphosulfate transporter 1Add BLAST. 432. Amino acid modifications. Feature key. Position(s). ... sp,Q8TB61,S35B2_HUMAN Adenosine 3-phospho 5-phosphosulfate transporter 1 OS=Homo sapiens OX=9606 GN=SLC35B2 PE=1 SV=1 ... Mediates the transport of adenosine 3-phospho 5-phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl ...
... phosphosulfate reductase (APR) catalyzes the two-electron reduction of adenosine 5-phosphosulfate to sulfite and AMP, which ... Thus, plant assimilatory APR represents a novel type of adenosine 5-phosphosulfate reductase with a [4Fe-4S] center as the ... sole cofactor, which is clearly different from the dissimilatory adenosine 5-phosphosulfate reductases found in sulfate ... Adenosine 5-phosphosulfate reductase (APR) catalyzes the two-electron reduction of adenosine 5-phosphosulfate to sulfite and ...
Adenosine-5-Phosphosulfate- and Sulfite Reductases Activities of Sulfate-Reducing Bacteria from Various Environments. Autoři. ... Adenosine-5-Phosphosulfate- and Sulfite Reductases Activities of Sulfate-Reducing Bacteria from Various Environments ...
Kopriva S, Koprivova A (2004) Plant adenosine 5′phosphosulfate reductase-the past, the present, and the future. J Exp Bot 55: ... Brunold C, Suter M (1990) Adenosine 5′-phosphosulphate sulfotransferase. In P Lea, ed, Methods in Plant Biochemistry, Vol 3. ... Sulfate taken up into plant cells has to be activated first by adenylation to adenosine 5′-phosphosulfate (APS) catalyzed by ... Complex Signaling Network in Regulation of Adenosine 5′-Phosphosulfate Reductase by Salt Stress in Arabidopsis Roots. Anna ...
Adenosine 3ʹ-Phosphate 5ʹ-Phosphosulfate, Tetralithium Salt CAS 102029-54-9 - Find MSDS or SDS, a COA, data sheets and more ... Adenosine 3ʹ-Phosphate 5ʹ-Phosphosulfate, Tetralithium Salt. 118410 Adenosine 3ʹ-Phosphate 5ʹ-Phosphosulfate, Tetralithium Salt ...
Adenosine 3-phosphate 5-phosphosulfate (PAPS), the sulfate donor in these reactions, must be transported from the cytosol, ... transporter by a combination of conventional and affinity chromatography as well as photoaffinity radiolabeling with adenosine ... Disruption of adenosine-5-phosphosulfate kinase in Arabidopsis reduces levels of sulfated secondary metabolites.. *Sarah G. ... Adenosine 3-phosphate 5-phosphosulfate (PAPS), the sulfate donor in these reactions, must be transported from the cytosol, ...
T2 - Kinetic Properties of the Adenosine Triphosphate Sulfurylase and Adenosine 5′-Phosphosulfate Kinase Domains ... Kinetic Properties of the Adenosine Triphosphate Sulfurylase and Adenosine 5′-Phosphosulfate Kinase Domains. Biochemistry, 43( ... Kinetic Properties of the Adenosine Triphosphate Sulfurylase and Adenosine 5′-Phosphosulfate Kinase Domains. / Lansdon, Eric B. ... Kinetic Properties of the Adenosine Triphosphate Sulfurylase and Adenosine 5′-Phosphosulfate Kinase Domains, Biochemistry, vol ...
The iron-sulfur flavoenzyme adenosine-5-phosphosulfate (APS) reductase catalyzes a key reaction of the global sulfur cycle by ... The iron-sulfur flavoenzyme adenosine-5-phosphosulfate (APS) reductase catalyzes a key reaction of the global sulfur cycle by ... Reaction Mechanism of the Iron−Sulfur Flavoenzyme Adenosine-5-Phosphosulfate Reductase Based on the Structural ... Reaction Mechanism of the Iron−Sulfur Flavoenzyme Adenosine-5-Phosphosulfate Reductase Based on the Structural ...
Crystal structure of adenosine 5\-phosphosulfate kinase from Arabidopsis Thaliana in Complex with AMPPNP and APS. Help ...
Replica of Adenosine 5´-Phosphosulfate. reference substance. supplier. supplier code. lot. Adenosine 5´-Phosphosulfate. Sigma. ... Details Adenosine 5´-Phosphosulfate. rotate: click+drag; translate: alt. +click+drag; zoom: mousewheel; save: mol ... Metabolites mapped to Adenosine 5´-Phosphosulfate. metabolite. stereoisomer. isotopomer. Adenosine 5-phosphosulfate. ambient ...
... phosphosulfate and 3′-phospho-adenosine 5′-phosphosulfate. Together they form a unique fingerprint. * Adenosine Phosphosulfate ... phosphosulfate and 3′-phospho-adenosine 5′-phosphosulfate. Biochimica et Biophysica Acta - Bioenergetics, 1837(2), 326-334. ... phosphosulfate and 3′-phospho-adenosine 5′-phosphosulfate, Biochimica et Biophysica Acta - Bioenergetics, vol. 1837, no. 2, pp ... phosphosulfate and 3′-phospho-adenosine 5′-phosphosulfate. Biochimica et Biophysica Acta - Bioenergetics. 2014 Feb;1837(2):326- ...
Slalom encodes an adenosine 3-phosphate 5-phosphosulfate transporter essential for development in Drosophila. Citation:. ... Slalom encodes an adenosine 3-phosphate 5-phosphosulfate transporter essential for development in Drosophila. EMBO J. 2003;22 ... phosphosulfate from the cytosol into the Golgi lumen. Mutations in this gene, which we call slalom, display defects in Wg and ... phosphosulfate. Here we report the first molecular identification of a gene that encodes a transmembrane protein required to ...
Adenosine-5-phosphosulfate reductase (APSR) is an iron-sulfur protein that catalyzes the reduction of adenosine-5- ... Geometric and electrostatic study of the [4Fe-4S] cluster of adenosine-5-phosphosulfate reductase from broken symmetry density ... phosphosulfate (APS) to sulfite. APSR coordinates to a [4Fe-4S] cluster via a conserved CC-X(~80)-CXXC motif, and the cluster ...
... leaving the product adenosine 5′-phosphosulfate (APS) in solution with an 80% yield. This separation procedure, coupled with ... phosphosulfate (PAPS) under the assay conditions adopted, so that the 35S remaining in solution after ethanol precipitation of ... A new assay for ATP sulfurylase based on differential solubility of the sodium salts of adenosine 5′-phosphosulfate and sulfate ... leaving the product adenosine 5′-phosphosulfate (APS) in solution with an 80% yield. This separation procedure, coupled with ...
Activity and stability of recombinant bifunctional rearranged and monofunctional domains of ATP-sulfurylase and adenosine 5- ... Murine adenosine 3-phosphate 5-phosphosulfate (PAPS) synthetase consists of a COOH-terminal ATP-sulfurylase domain covalently ... linked through a nonhomologous intervening sequence to an NH2-terminal adenosine 5-phosphosulfate (APS) kinase domain forming ... Activity and stability of recombinant bifunctional rearranged and monofunctional domains of ATP-sulfurylase and adenosine 5- ...
APS1-4, ATP sulfurylases 1-4; APR1-4, adenosine phosphosulfate reductases 1-4; GSH1 & 2, GSH synthetase 1 & 2; OASTL, O- ... 2010) Sulphur flux through the sulphate assimilation pathway is differently controlled by adenosine 5′-phosphosulphate ... adenosine 5′-phosphosulfate (APS)] via a well-known enzymatic pathway (Fig. S4). Gene expression analysis based on RNA- ...
In the presence of adenosine 3′-phosphate 5′-phosphosulfate (PAPS), 3-OST-1 converts [35S]HS, lacking 3-O-sulfates, into [35S] ... adenosine 3′-phosphate 5′-phosphosulfate (PAPS); heparin cofactor II (HCII); cardiac microvascular endothelial (CME); alkaline ...
Described as one of the primary regulatory points in sulfate reduction, adenosine 5′-phosphosulfate (APS) is reduced to sulfite ... Interestingly, expression studies of adenosine 5′-phosphosulfate reductase (APR) indicate that sulfur assimilation is ...
Adenosine 3-phospho 5-phosphosulfate transporter 1 Protein Accession:. Q8TB61. Secondary Accessions: *B4DDM2 ... 3-phosphoadenosine 5-phosphosulfate transport. IDA. 12716889. GO:0050428. 3-phosphoadenosine 5-phosphosulfate biosynthetic ... Mediates the transport of adenosine 3-phospho 5-phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl ... Mediates the transport of adenosine 3-phospho 5-phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl ...
... phosphosulfate kinase, adenosine phosphosulfate kinase, adenosine phosphosulfokinase, adenosine-5-phosphosulfate-3- ... MacRae IJ, Rose AB, Segel IH (October 1998). "Adenosine 5-phosphosulfate kinase from Penicillium chrysogenum. site-directed ... Other names in common use include adenylylsulfate kinase (phosphorylating), 5-phosphoadenosine sulfate kinase, adenosine 5- ... phosphosulfate synthase and its functional domains". J. Biol. Chem. 273 (30): 19311-20. doi:10.1074/jbc.273.30.19311. PMID ...
Psr, polysulfide reductase; Sox, Sox multienzyme system; Dsr, dissimilatory sulfite reductase; Apr, adenosine 5′-phosphosulfate ...
APS, adenosine 5′-phosphosulphate; ATPS, ATP sulphurylase; APK, APS kinase; APS, adenosine phosphosulphate; Ind, indole; Glc, ... Biosynthesis and sulfonylation of GLS requires glutathione (GSH) and the sulfur donor 5′-phosphosulphate (PAPS). The reaction ... phosphosulfate to the cytosol. Plant Cell 24, 4187-4204. (doi:10.1105/tpc.112.101964). ... phosphosulphate (PAPS), whose by-product phosphoadenosine 5′-phosphate (PAP) has been shown to mediate retrograde signals from ...
Adenosine 3-phosphate 5-phosphosulfate*Adenosine 3-phosphate 5-sulfatophosphate*Adenosine 5-phosphosulfate 3-phosphate* ... 3-PHOSPHATE-ADENOSINE-5-PHOSPHATE SULFATE*3-Phospho-5-adenylyl sulfate*3-Phosphoadenosine 5-phosphosulfate*3- ... Adenosine 3,5-diphosphate, 5-anhydride with sulfuric acid*Adenosine 3,5-diphosphate, 5-anhydride with sulfuric acid (6CI ... Adenosine, 3,5-bis(dihydrogen phosphate) , 5-monoanhydride with sulfuric acid*Adenosine, 3,5-bis(dihydrogen phosphate) , 5 ...
APS, adenosine-5′-phosphosulfate; GLS, glucosinolate; APK, APS KINASE; APR, APS REDUCTASE; GSH1, GLU-CYS LIGASE. B, Circadian ...
Characterization of Adenosine 5′-Phospho-Sulfate Kinase (APSK) Genes from Higher Plants ...
dsr, dissimilatory sulfite reductase gene cluster; sox, sulfur oxidation gene cluster; aprBA, adenosine 5′-phosphosulfate (APS ... Phylogeny of the alpha and beta subunits of the dissimilatory adenosine-5′-phosphosulfate (APS) reductase from sulfate-reducing ... Molecular analysis of the distribution and phylogeny of dissimilatory adenosine-5′-phosphosulfate reductase-encoding genes ( ... and the adenosine 5′-phosphosulfate (APS) reductase (apr) were present throughout the OMZ (Fig. 2B). Several of the proteins ...
ATP sulfurylase/adenosine 5 phosphosulfate kinase. *ATP SULFURYLASE/ADENOSINE 5-PRIME PHOSPHOSULFATE KINASE 2 ... Bifunctional 3 phosphoadenosine 5 phosphosulfate synthase 2. *Bifunctional 3 phosphoadenosine 5 phosphosulfate synthethase ... In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 ... Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) ...
Phosphosulfate Synthase 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human ... synthesis of adenosine 5-phosphosulfate and phosphorylation of APS to generate 3-phospho-adenosine 5-phosphosulfate (PAPS), ... The first step is the transfer of a sulfate group to ATP to yield adenosine 5-phosphosulfate (APS), and the second step is the ... The first step is the transfer of a sulfate group to ATP to yield adenosine 5-phosphosulfate (APS), and the second step is the ...
  • Mediates the transport of adenosine 3'-phospho 5'-phosphosulfate (PAPS), from cytosol into Golgi. (uniprot.org)
  • Adenosine 3'-phosphate 5'-phosphosulfate (PAPS), the sulfate donor in these reactions, must be transported from the cytosol, its site of synthesis, into the lumen of the Golgi apparatus. (semanticscholar.org)
  • Recombinant human 3′-phosphoadenosine 5′-phosphosulfate (PAPS) synthetase, isoform 1 (brain), was purified to near-homogeneity from an Escherichia coli expression system and kinetically characterized. (elsevier.com)
  • The native enzyme, a dimer with each 71 kDa subunit containing an adenosine triphosphate (ATP) sulfurylase and an adenosine 5′-phosphosulfate (APS) kinase domain, catalyzes the overall formation of PAPS from ATP and inorganic sulfate. (elsevier.com)
  • Murine adenosine 3'-phosphate 5'-phosphosulfate (PAPS) synthetase consists of a COOH-terminal ATP-sulfurylase domain covalently linked through a nonhomologous intervening sequence to an NH2-terminal adenosine 5'-phosphosulfate (APS) kinase domain forming a bifunctional fused protein. (duke.edu)
  • MIM 608359) use an activated form of sulfate, 3-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS), as a common sulfate donor for sulfation of glycoproteins, proteoglycans, and glycolipids in the endoplasmic reticulum and Golgi apparatus. (genecards.org)
  • Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes. (abcam.com)
  • In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2. (abcam.com)
  • In mammals, the sulfate source is 3'-phosphoadenosine 5'-phosphosulfate (PAPS), created from ATP and inorganic sulfate. (genecards.org)
  • The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). (genecards.org)
  • Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. (hmdb.ca)
  • The reduction of sulfate in nature involves the formation of one or sometimes two sulfate esters, adenosine 5'-phosphosulfate (APS) and 3'-phosphoadenosine-5'-phosphosulfate (PAPS). (wikipedia.org)
  • These were incubated with NOHAAF or NOHGAF in the presence or absence of 3'-phospho-adenosine-5'-phosphosulfate (PAPS). (cdc.gov)
  • The human phosphoadenosine-phosphosulfate synthase (PAPS) system is a bifunctional enzyme: ATP sulfurylase, which catalyzes the formation of adenosine 5'-phosphosulfate (APS) from ATP and inorganic sulfate and the second step is catalyzed by the APS kinase portion of 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthase, which involves the formation of PAPS from enzyme bound APS and ATP. (expasy.org)
  • The universal sulfuryl group donor for SULT-catalyzed sulfation is adenosine 3'-phosphate 5'-phosphosulfate (PAPS), whereas the reaction products are a sulfated product and adenosine 3',5'-diphosphate (PAP). (sigmaaldrich.com)
  • Adenosine 5'-phosphosulfate reductase (APR) catalyzes the two-electron reduction of adenosine 5'-phosphosulfate to sulfite and AMP, which represents the key step of sulfate assimilation in higher plants. (rero.ch)
  • Thus, plant assimilatory APR represents a novel type of adenosine 5'-phosphosulfate reductase with a [4Fe-4S] center as the sole cofactor, which is clearly different from the dissimilatory adenosine 5'-phosphosulfate reductases found in sulfate reducing bacteria. (rero.ch)
  • Using known Arabidopsis ( Arabidopsis thaliana ) mutants in signaling pathways, we analyzed regulation of the key enzyme of sulfate assimilation, adenosine 5′-phosphosulfate reductase (APR), by salt stress. (plantphysiol.org)
  • The iron-sulfur flavoenzyme adenosine-5'-phosphosulfate (APS) reductase catalyzes a key reaction of the global sulfur cycle by reversibly transforming APS to sulfite and AMP. (uni-konstanz.de)
  • Adenosine-5'-phosphosulfate reductase (APSR) is an iron-sulfur protein that catalyzes the reduction of adenosine-5'-phosphosulfate (APS) to sulfite. (scripps.edu)
  • The iron-sulfur flavoenzyme adenylylsulfate (adenosine 5′-phosphosulfate, APS) reductase, a key enzyme in the microbial dissimilatory sulfate reduction, has been purified in three chromatographic steps(DEAE-Biogel A, Source 15, and Superdex 200 columns). (mendeley.com)
  • Sulfate taken up into plant cells has to be activated first by adenylation to adenosine 5′-phosphosulfate (APS) catalyzed by ATP sulfurylase. (plantphysiol.org)
  • Segel, Irwin H. / Human 3′-Phosphoadenosine 5′-Phosphosulfate Synthetase (Isoform 1, Brain) : Kinetic Properties of the Adenosine Triphosphate Sulfurylase and Adenosine 5′-Phosphosulfate Kinase Domains . (elsevier.com)
  • A new assay for ATP sulfurylase (ATP:sulfate adenylyltransferase, EC 2.7.7.4) has been devised, the key feature of which is the quantitative precipitation of the unreacted substrate as Na 2SO 4, in ethanol:water (5:1), leaving the product adenosine 5′-phosphosulfate (APS) in solution with an 80% yield. (naver.com)
  • Activity and stability of recombinant bifunctional rearranged and monofunctional domains of ATP-sulfurylase and adenosine 5'-phosphosulfate kinase. (duke.edu)
  • The hybridized primer and template strand is incubated with enzyme DNA polymerase, luciferin, luciferase, apyrase, ATP sulfurylase, with substrates adenosine 5' phosphosulfate (APS). (openpr.com)
  • ATP sulfurylase converts PPi to ATP in the presence of adenosine 5' phosphosulfate (APS). (openpr.com)
  • ATP Sulfurylase ( NEB #M0394 ) catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5´-phosphosulfate (APS) and pyrophosphate. (neb.com)
  • Phosphoadenosine Phosphosulfate" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
  • This graph shows the total number of publications written about "Phosphoadenosine Phosphosulfate" by people in this website by year, and whether "Phosphoadenosine Phosphosulfate" was a major or minor topic of these publications. (umassmed.edu)
  • Below are the most recent publications written about "Phosphoadenosine Phosphosulfate" by people in Profiles. (umassmed.edu)
  • Purification of the Golgi adenosine 3'-phosphate 5'-phosphosulfate transporter, a homodimer within the membrane. (semanticscholar.org)
  • It transported APS, 3′-phospho-adenosine 5′-phosphosulfate, sulfate and phosphate almost exclusively by a counter-exchange mechanism. (elsevier.com)
  • Slalom encodes an adenosine 3'-phosphate 5'-phosphosulfate transporter essential for development in Drosophila. (harvard.edu)
  • Sulfation of all macromolecules entering the secretory pathway in higher organisms occurs in the Golgi and requires the high-energy sulfate donor adenosine 3'-phosphate 5'-phosphosulfate. (harvard.edu)
  • Here we report the first molecular identification of a gene that encodes a transmembrane protein required to transport adenosine 3'-phosphate 5'-phosphosulfate from the cytosol into the Golgi lumen. (harvard.edu)
  • The common sulfate group donor is adenosine 3'-phosphate 5'-phosphosulfate. (mpbio.com)
  • Milla ME, Hirschberg CB (1989) Reconstitution of Golgi vesicle CMP-sialic acid and adenosine 3′-phosphate 5′-phosphosulfate transport into proteoliposomes. (springer.com)
  • Mammalian EST sulfurylates the hydroxyl group of estrogenic steroids by transferring the sulfate from a cosubstrate adenosine 3 prime-phosphate-5 prime-phosphosulfate. (novusbio.com)
  • Heparan-sulphate 6-O-sulphotransferase (HS6ST), which catalyses the transfer of sulphate from adenosine 3'-phosphate, 5'-phosphosulphate to the 6th position of the N -sulphoglucosamine residue in heparan sulphate [ PMID: 12492399 ]. (ebi.ac.uk)
  • Reactions were initiated by the addition of nicotinamide adenine dinucleotide phosphate reduced form (NADPH), uridine diphosphoglucuronic acid (UDPGA), and adenosine phosphate phosphosulfate (APPS) to give the final cofactor concentrations of 2 mM, 4 mM, and 4 mM, respectively. (bio-medicine.org)
  • PAPSS2 (3'-Phosphoadenosine 5'-Phosphosulfate Synthase 2) is a Protein Coding gene. (genecards.org)
  • Adenosine 3'-phospho 5'-phosphosulfate transporter 1 is a protein that in humans is encoded by the SLC35B2 gene. (wikipedia.org)
  • GO annotations related to this gene include signal transducer activity and 3-phosphoadenosine 5-phosphosulfate transmembrane transporter activity . (genecards.org)
  • Adenosine 5'- phosphosulfate sulfotransferase from the marine macroalga Porphyra yezoensis Ueda (Rhodophyta): Stabilization, purification, and properties. (bio.net)
  • Purine-induced block to mouse embryo cleavage is reversed by compounds that elevate cyclic adenosine monophosphate. (umassmed.edu)
  • An enzyme that catalyzes the activation of sulfate ions by ATP to form adenosine-5'-phosphosulfate and pyrophosphate. (bioportfolio.com)
  • Satishchandran C, Hickman Y, Markham G. Characterization of the phosphorylated enzyme intermediate formed in the adenosine 5'-phosphosulfate kinase reaction. (labome.org)
  • Other names in common use include adenylylsulfate kinase (phosphorylating), 5'-phosphoadenosine sulfate kinase, adenosine 5'-phosphosulfate kinase, adenosine phosphosulfate kinase, adenosine phosphosulfokinase, adenosine-5'-phosphosulfate-3'-phosphokinase, and APS kinase. (wikipedia.org)
  • Disruption of adenosine-5'-phosphosulfate kinase in Arabidopsis reduces levels of sulfated secondary metabolites. (semanticscholar.org)
  • Adenosine 5'-phosphosulfate kinase from Penicillium chrysogenum. (wikipedia.org)
  • The putative PAPX products decompose to adenosine 3′,5′ -diphosphate and the original oxyanion. (elsevier.com)
  • Herrmann J, Nathin D, Lee SG, Sun T, Jez JM (2015) Recapitulating the structural evolution of redox regulation in adenosine-5'-phosphosulfate kinase from cyanobacteria to plants. (wustl.edu)
  • In this study, the identification of the mitochondrial carrier for adenosine 5′-phosphosulfate (APS) is described. (elsevier.com)
  • The human phosphoadenosine-phosphosulfate synthase (PAPS) system is a bifunctional enzyme: ATP sulfurylase, which catalyzes the formation of adenosine 5'-phosphosulfate (APS) from ATP and inorganic sulfate and the second step is catalyzed by the APS kinase portion of 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthase, which involves the formation of PAPS from enzyme bound APS and ATP. (expasy.org)
  • In particular, Estrone and phosphoadenosine phosphosulfate can be converted into estrone sulfate and adenosine 3',5'-diphosphate through the action of the enzyme estrogen sulfotransferase. (bovinedb.ca)