Adenosine Phosphosulfate: 5'-Adenylic acid, monoanhydride with sulfuric acid. The initial compound formed by the action of ATP sulfurylase on sulfate ions after sulfate uptake. Synonyms: adenosine sulfatophosphate; APS.Sulfate Adenylyltransferase: An enzyme that catalyzes the activation of sulfate ions by ATP to form adenosine-5'-phosphosulfate and pyrophosphate. This reaction constitutes the first enzymatic step in sulfate utilization following the uptake of sulfate. EC 2.7.7.4.Adenosine: A nucleoside that is composed of ADENINE and D-RIBOSE. Adenosine or adenosine derivatives play many important biological roles in addition to being components of DNA and RNA. Adenosine itself is a neurotransmitter.Phosphoadenosine Phosphosulfate: 3'-Phosphoadenosine-5'-phosphosulfate. Key intermediate in the formation by living cells of sulfate esters of phenols, alcohols, steroids, sulfated polysaccharides, and simple esters, such as choline sulfate. It is formed from sulfate ion and ATP in a two-step process. This compound also is an important step in the process of sulfur fixation in plants and microorganisms.Receptor, Adenosine A2A: A subclass of adenosine A2 receptors found in LEUKOCYTES, the SPLEEN, the THYMUS and a variety of other tissues. It is generally considered to be a receptor for ADENOSINE that couples to the GS, STIMULATORY G-PROTEIN.Receptor, Adenosine A1: A subtype of ADENOSINE RECEPTOR that is found expressed in a variety of tissues including the BRAIN and DORSAL HORN NEURONS. The receptor is generally considered to be coupled to the GI, INHIBITORY G-PROTEIN which causes down regulation of CYCLIC AMP.Adenosine Deaminase: An enzyme that catalyzes the hydrolysis of ADENOSINE to INOSINE with the elimination of AMMONIA.Receptor, Adenosine A3: A subtype of ADENOSINE RECEPTOR that is found expressed in a variety of locations including the BRAIN and endocrine tissues. The receptor is generally considered to be coupled to the GI, INHIBITORY G-PROTEIN which causes down regulation of CYCLIC AMP.Receptor, Adenosine A2B: A subclass of adenosine A2 receptors found in the CECUM, the COLON, the BLADDER, and a variety of other tissues. It is generally considered to be a low affinity receptor for ADENOSINE that couples to the GS, STIMULATORY G-PROTEIN.Adenosine Kinase: An enzyme that catalyzes the formation of ADP plus AMP from adenosine plus ATP. It can serve as a salvage mechanism for returning adenosine to nucleic acids. EC 2.7.1.20.Receptors, Adenosine A2: A subclass of ADENOSINE RECEPTORS that are generally considered to be coupled to the GS, STIMULATORY G-PROTEIN which causes up regulation of CYCLIC AMP.Adenosine A2 Receptor Agonists: Compounds that selectively bind to and activate ADENOSINE A2 RECEPTORS.
(1/37) Reduction of adenosine-5'-phosphosulfate instead of 3'-phosphoadenosine-5'-phosphosulfate in cysteine biosynthesis by Rhizobium meliloti and other members of the family Rhizobiaceae.

We have cloned and sequenced three genes from Rhizobium meliloti (Sinorhizobium meliloti) that are involved in sulfate activation for cysteine biosynthesis. Two of the genes display homology to the Escherichia coli cysDN genes, which code for an ATP sulfurylase (EC 2.7.7.4). The third gene has homology to the E. coli cysH gene, a 3'-phosphoadenosine-5'-phosphosulfate (PAPS) reductase (EC 1.8.99.4), but has greater homology to a set of genes found in Arabidopsis thaliana that encode an adenosine-5'-phosphosulfate (APS) reductase. In order to determine the specificity of the R. meliloti reductase, the R. meliloti cysH homolog was histidine tagged and purified, and its specificity was assayed in vitro. Like the A. thaliana reductases, the histidine-tagged R. meliloti cysH gene product appears to favor APS over PAPS as a substrate, with a Km for APS of 3 to 4 microM but a Km for PAPS of >100 microM. In order to determine whether this preference for APS is unique to R. meliloti among members of the family Rhizobiaceae or is more widespread, cell extracts from R. leguminosarum, Rhizobium sp. strain NGR234, Rhizobium fredii (Sinorhizobium fredii), and Agrobacterium tumefaciens were assayed for APS or PAPS reductase activity. Cell extracts from all four species also preferentially reduce APS over PAPS.  (+info)

(2/37) Identification of a new class of 5'-adenylylsulfate (APS) reductases from sulfate-assimilating bacteria.

A gene was cloned from Burkholderia cepacia DBO1 that is homologous with Escherichia coli cysH encoding 3'-phosphoadenylylsulfate (PAPS) reductase. The B. cepacia gene is the most recent addition to a growing list of cysH homologs from a diverse group of sulfate-assimilating bacteria whose products show greater homology to plant 5'-adenylylsulfate (APS) reductase than they do to E. coli CysH. The evidence reported here shows that the cysH from one of the species, Pseudomonas aeruginosa, encodes APS reductase. It is able to complement an E. coli cysH mutant and a cysC mutant, indicating that the enzyme is able to bypass PAPS, synthesized by the cysC product. Insertional knockout mutation of P. aeruginosa cysH produced cysteine auxotrophy, indicating its role in sulfate assimilation. Purified P. aeruginosa CysH expressed as a His-tagged recombinant protein is able to reduce APS, but not PAPS. The enzyme has a specific activity of 5.8 micromol. min(-1). mg of protein(-1) at pH 8.5 and 30 degrees C with thioredoxin supplied as an electron donor. APS reductase activity was detected in several bacterial species from which the novel type of cysH has been cloned, indicating that this enzyme may be widespread. Although an APS reductase from dissimilatory sulfate-reducing bacteria is known, it shows no structural or sequence homology with the assimilatory-type APS reductase reported here. The results suggest that the dissimilatory and assimilatory APS reductases evolved convergently.  (+info)

(3/37) Effects of AMP derivatives on cyclic AMP levels in NG108-15 cells.

1. In NG108-15 neuroblastomaxglioma hybrid cells, ATP stimulates intracellular cyclic AMP formation, which is inhibited by both adenosine (P(1)) and P2 receptor antagonists. In the present study, we examined the effects of several AMP derivatives in NG108-15 cells and mouse neuroblastoma N18TG-2 cells. 2. Adenosine 2'-monophosphate (A2P), adenosine 3'-monophosphate (A3P) and adenosine 5'-phosphosulphate (A5PS) increased cyclic AMP levels with similar concentration-dependencies in NG108-15 cells. 3. Increases in cyclic AMP by AMP derivatives were inhibited by the P2 receptor antagonist PPADS, but not by suramin. Effects of AMP derivatives were also inhibited by P(1) receptor antagonists ZM241385, XAC, DPCPX and partially by alloxazine. The ecto-nucleotidase inhibitor alpha, beta-methyleneADP was without effect. 4. In contrast, AMP derivatives did not change cyclic AMP levels in N18TG-2 cells. Accumulation of cyclic AMP in N18TG-2 cells was stimulated by adenosine A(2) receptor agonists CGS21680 and NECA, but not by ATP or beta, gamma-methyleneATP, agonists for cyclic AMP production in NG108-15 cells. 5. Reverse transcription-coupled polymerase chain reaction (RT - PCR) analyses revealed that N18TG-2 cells express both A(2A) and A(2B) receptors, while NG108-15 cells express mainly A(2A) receptors. 6. AMP derivatives did not affect the P2X and P2Y receptors expressed in NG108-15 cells. 7. These results suggest that A2P, A3P and A5PS act as agonists for cyclic AMP production and that these compounds are valuable tools for determinating the mechanism of ATP-stimulated cyclic AMP response in NG108-15 cells.  (+info)

(4/37) Sulfate assimilation in higher plants characterization of a stable intermediate in the adenosine 5'-phosphosulfate reductase reaction.

The enzyme catalysing the reduction of adenosine 5'-phosphosulfate (AdoPS) to sulfite in higher plants, AdoPS reductase, is considered to be the key enzyme of assimilatory sulfate reduction. In order to address its reaction mechanism, the APR2 isoform of this enzyme from Arabidopsis thaliana was overexpressed in Escherichia coli and purified to homogeneity. Incubation of the enzyme with [35S]AdoPS at 4 degrees C resulted in radioactive labelling of the protein. Analysis of APR2 tryptic peptides revealed 35SO2-3 bound to Cys248, the only Cys conserved between AdoPS and prokaryotic phosphoadenosine 5'-phosphosulfate reductases. Consistent with this result, radioactivity could be released from the protein by incubation with thiols, inorganic sulfide and sulfite. The intermediate remained stable, however, after incubation with sulfate, oxidized glutathione or AdoPS. Because truncated APR2, missing the thioredoxin-like C-terminal part, could be labelled even at 37 degrees C, and because this intermediate was more stable than the complete protein, we conclude that the thioredoxin-like domain was required to release the bound SO2-3 from the intermediate. Taken together, these results demonstrate for the first time the binding of 35SO2-3 from [35S]AdoPS to AdoPS reductase and its subsequent release, and thus contribute to our understanding of the molecular mechanism of AdoPS reduction in plants.  (+info)

(5/37) Crystal structure of ATP sulfurylase from Saccharomyces cerevisiae, a key enzyme in sulfate activation.

ATP sulfurylases (ATPSs) are ubiquitous enzymes that catalyse the primary step of intracellular sulfate activation: the reaction of inorganic sulfate with ATP to form adenosine-5'-phosphosulfate (APS) and pyrophosphate (PPi). With the crystal structure of ATPS from the yeast Saccharomyces cerevisiae, we have solved the first structure of a member of the ATP sulfurylase family. We have analysed the crystal structure of the native enzyme at 1.95 Angstroms resolution using multiple isomorphous replacement (MIR) and, subsequently, the ternary enzyme product complex with APS and PPi bound to the active site. The enzyme consists of six identical subunits arranged in two stacked rings in a D:3 symmetric assembly. Nucleotide binding causes significant conformational changes, which lead to a rigid body structural displacement of domains III and IV of the ATPS monomer. Despite having similar folds and active site design, examination of the active site of ATPS and comparison with known structures of related nucleotidylyl transferases reveal a novel ATP binding mode that is peculiar to ATP sulfurylases.  (+info)

(6/37) Identification of yacE (coaE) as the structural gene for dephosphocoenzyme A kinase in Escherichia coli K-12.

Dephosphocoenzyme A (dephospho-CoA) kinase catalyzes the final step in coenzyme A biosynthesis, the phosphorylation of the 3'-hydroxy group of the ribose sugar moiety. Wild-type dephospho-CoA kinase from Corynebacterium ammoniagenes was purified to homogeneity and subjected to N-terminal sequence analysis. A BLAST search identified a gene from Escherichia coli previously designated yacE encoding a highly homologous protein. Amplification of the gene and overexpression yielded recombinant dephospho-CoA kinase as a 22.6-kDa monomer. Enzyme assay and nuclear magnetic resonance analyses of the product demonstrated that the recombinant enzyme is indeed dephospho-CoA kinase. The activities with adenosine, AMP, and adenosine phosphosulfate were 4 to 8% of the activity with dephospho-CoA. Homologues of the E. coli dephospho-CoA kinase were identified in a diverse range of organisms.  (+info)

(7/37) Assay of adenosine 3'-phosphate 5'-sulphatophosphate in hepatic tissues.

A fluorimetric assay, based on the ability of boiled hepatic extracts to support the sulphO-conjugation of harmol, was used to demonstrate and quantify PAdoPS (adenosine 3'-phosphate 5'-sulphatophosphate) present in liver. A stoicheiometric relationship was established between the sulphate conjugate formed and the 'active sulphate' utilized. Guinea-pig, rat, mouse and rabbit livers contain 3.3, 2.9, 0.8 and 0.5 mumol of PAdoPS/100 G wet wt. respectively.  (+info)

(8/37) The presence of an iron-sulfur cluster in adenosine 5'-phosphosulfate reductase separates organisms utilizing adenosine 5'-phosphosulfate and phosphoadenosine 5'-phosphosulfate for sulfate assimilation.

It was generally accepted that plants, algae, and phototrophic bacteria use adenosine 5'-phosphosulfate (APS) for assimilatory sulfate reduction, whereas bacteria and fungi use phosphoadenosine 5'-phosphosulfate (PAPS). The corresponding enzymes, APS and PAPS reductase, share 25-30% identical amino acids. Phylogenetic analysis of APS and PAPS reductase amino acid sequences from different organisms, which were retrieved from the GenBank(TM), revealed two clusters. The first cluster comprised known PAPS reductases from enteric bacteria, cyanobacteria, and yeast. On the other hand, plant APS reductase sequences were clustered together with many bacterial ones, including those from Pseudomonas and Rhizobium. The gene for APS reductase cloned from the APS-reducing cyanobacterium Plectonema also clustered together with the plant sequences, confirming that the two classes of sequences represent PAPS and APS reductases, respectively. Compared with the PAPS reductase, all sequences of the APS reductase cluster contained two additional cysteine pairs homologous to the cysteine residues involved in binding an iron-sulfur cluster in plants. Mossbauer analysis revealed that the recombinant APS reductase from Pseudomonas aeruginosa contains a [4Fe-4S] cluster with the same characteristics as the plant enzyme. We conclude, therefore, that the presence of an iron-sulfur cluster determines the APS specificity of the sulfate-reducing enzymes and thus separates the APS- and PAPS-dependent assimilatory sulfate reduction pathways.  (+info)

*  Adenylyl-sulfate reductase (thioredoxin)
Abola AP, Willits MG, Wang RC, Long SR (1999). "Reduction of Adenosine-5′-Phosphosulfate Instead of 3′-Phosphoadenosine-5′- ... The systematic name of this enzyme class is AMP,sulfite:thioredoxin-disulfide oxidoreductase (adenosine-5'-phosphosulfate- ... Williams SJ, Senaratne RH, Mougous JD, Riley LW, Bertozzi CR (2002). "5'-adenosinephosphosulfate lies at a metabolic branch ... Phosphosulfate in Cysteine Biosynthesis by Rhizobium meliloti and Other Members of the Family Rhizobiaceae". J. Bacteriol. 181 ...
*  Adenylyl-sulfate reductase
... phosphosulfate-forming). Other names in common use include adenosine phosphosulfate reductase, adenosine 5'-phosphosulfate ... reductase, APS-reductase, APS reductase, AMP, sulfite:(acceptor) oxidoreductase, and (adenosine-5'-phosphosulfate-forming). ... The systematic name of this enzyme class is AMP,sulfite:acceptor oxidoreductase (adenosine-5'- ...
*  Adenylyl-sulfate kinase
... phosphosulfate kinase, adenosine phosphosulfate kinase, adenosine phosphosulfokinase, adenosine-5'-phosphosulfate-3'- ... MacRae IJ, Rose AB, Segel IH (October 1998). "Adenosine 5'-phosphosulfate kinase from Penicillium chrysogenum. site-directed ... Other names in common use include adenylylsulfate kinase (phosphorylating), 5'-phosphoadenosine sulfate kinase, adenosine 5'- ... phosphosulfate synthase and its functional domains". J. Biol. Chem. 273 (30): 19311-20. doi:10.1074/jbc.273.30.19311. PMID ...
*  Adenylylsulfatase
Other names in common use include adenosine 5-phosphosulfate sulfohydrolase, and adenylylsulfate sulfohydrolase. This enzyme ...
*  Jiayang Li
Li, J. Y.; Schiff, J. A. (1991). "Purification and properties of adenosine 5'-phosphosulphate sulphotransferase from Euglena". ... Li, Jiayang (1991). Purification and properties of ATP sulfurylase and adenosine 5'-phosphosulfate sulfotransferase from ... ATP sulfurylase and adenosine 5'-phosphosulfate sulfotransferase) in the single-celled organism Euglena. Following his PhD, Li ...
*  Pyrosequencing
... and with the substrates adenosinephosphosulfate (APS) and luciferin. The addition of one of the four deoxynucleotide ... This incorporation releases pyrophosphate (PPi). ATP sulfurylase converts PPi to ATP in the presence of adenosine 5´ ... phosphosulfate. This ATP acts as a substrate for the luciferase-mediated conversion of luciferin to oxyluciferin that generates ...
*  3'(2'),5'-bisphosphate nucleotidase
... adenosine 5'phosphosulfate sulfohydrolase and adenosine-5'-phosphosulfate cyclase activities". Eur. J. Biochem. 65 (1): 113-21 ... adenosine 5'-phosphate + phosphate Thus, the two substrates of this enzyme are adenosine 3',5'-bisphosphate and H2O, whereas ... In enzymology, a 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) is an enzyme that catalyzes the chemical reaction adenosine 3 ... The systematic name of this enzyme class is adenosine-3'(2'),5'-bisphosphate 3'(2')-phosphohydrolase. Other names in common use ...
*  Adenosine 3',5'-bisphosphate
... phosphosulfate. This product is then hydrolysed by 3'(2'),5'-bisphosphate nucleotidase to give adenosine monophosphate, which ... Adenosine 3',5'-bisphosphate is a form of an adenosine nucleotide with two phosphate groups attached to different carbons in ... Adenosine 3',5'-bisphosphate is produced as a product of sulfotransferase enzymes from the donation of a sulfate group from the ... This is distinct from adenosine diphosphate, where the two phosphate groups are attached in a chain to the 5' carbon atom in ...
*  Sulfonucleotide reductase
The enzymes reduce adenosine-5'-phosphosulfate by nucleophilic attack to produce the sulfite product. This typically involves a ...
*  Thiol sulfotransferase
17 Reactions of the adenosine 5'-phosphosulfate (APS) sulfotransferase from Chlorella and studies of model reactions which ... Schmidt A (1976). "The adenosine-5'-phosphosulfate sulfotransferase from spinach (Spinacea oleracea L.). Stabilization, partial ... Other names in common use include phosphoadenylylsulfate-thiol sulfotransferase, PAPS sulfotransferase, and adenosine 3'- ... adenosine 3',5'-bisphosphate + an S-alkyl thiosulfate Thus, the two substrates of this enzyme are 3'-phosphoadenylyl sulfate ...
*  Sulfur assimilation
Sulfate needs to be activated to adenosine 5'-phosphosulfate (APS) prior to its reduction to sulfite. The activation of sulfate ...
*  3'-Phosphoadenosine-5'-phosphosulfate
... (PAPS) is a derivative of adenosine monophosphate that is phosphorylated at the 3' ... It is endogenously synthesized by organisms via the phosphorylation of adenosine 5'-phosphosulfate (APS), an intermediary ... In humans such reaction is performed by bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthases (PAPSS1 and PAPSS2) using ... Phosphosulfate Synthetase 1 (PAPSS1) and PAPSS2: Gene Cloning, Characterization and Chromosomal Localization". Biochemical and ...
*  Adenylyl-sulfate reductase (glutathione)
The systematic name of this enzyme class is AMP,sulfite:glutathione-disulfide oxidoreductase (adenosine-5'-phosphosulfate- ... adenosine-5'-phosphosulfate-forming), and plant-type 5'-adenylylsulfate reductase. Gutierrez-Marcos JF, Roberts MA, Campbell EI ... 2 glutathione The 3 substrates of this enzyme are adenosine monophosphate, sulfite, and glutathione disulfide, whereas its two ...
*  PAPSS1
... kinetic properties of the adenosine triphosphate sulfurylase and adenosine 5'-phosphosulfate kinase domains". Biochemistry. 43 ... Sekulic N, Konrad M, Lavie A (2007). "Structural mechanism for substrate inhibition of the adenosine 5'-phosphosulfate kinase ... 1998). "cDNA cloning, expression, and characterization of the human bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate ... In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 ...
*  PAPSS2
"Genomic organization of the mouse and human genes encoding the ATP sulfurylase/adenosine 5'-phosphosulfate kinase isoform SK2 ... "Entrez Gene: PAPSS2 3'-phosphoadenosine 5'-phosphosulfate synthase 2". Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale ... Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthetase 2 is an enzyme that in humans is encoded by the PAPSS2 gene. ... In mammals, the sulfate source is 3'-phosphoadenosine 5'-phosphosulfate (PAPS), created from ATP and inorganic sulfate. Two ...
*  Sulfate-reducing microorganisms
This is done by the enzyme ATP-sulfurylase, which uses ATP and sulfate to create adenosine 5'-phosphosulfate (APS). APS is ...
*  Sulfate conjugate
... usually adenosine 5'-phosphosulfate (APS) or 3'-phosphoadenosine-5'-phosphosulfate (PAPS). Sulfate esters may be hydrolyzed by ...
*  Carbohydrate sulfotransferase
ATP sulfurylase first generates adenosine-5'-phosphosulfate (APS) and then APS kinase transfers a phosphate from ATP to APS to ... Kamio, K.; Honke, K.; Makita, A. (Dec 1995). "Pyridoxal 5'-phosphate binds to a lysine residue in the adenosine 3'-phosphate 5 ... In eukaryotic cells the activated sulfate donor is 3'-phosphoadenosine-5'-phosphosulfate (PAPS) (Figure 1). PAPS is synthesized ... May 1997). "Sulfation and sulfotransferases 5: the importance of 3'-phosphoadenosine 5'-phosphosulfate (PAPS) in the regulation ...
*  Congregibacter litoralis
It is predicted that the potential use of the sox genes would be in the adenosine 5'-phosphosulfate (APS)/ 3'-Phosphoadenosine- ... 5'-phosphosulfate (PAPS) pathway. Though KT71 and other AAnPs can make up over 10% of the bacterioplankonic community in ...
*  Organosulfate
The reduction of sulfate in nature involves the formation of one or sometimes two sulfate esters, adenosine 5'-phosphosulfate ( ... APS) and 3'-phosphoadenosine-5'-phosphosulfate (PAPS). Sulfate is an inert anion, so nature activates it by the formation of ...
*  Microbial metabolism
... adenosine 5'-phosphosulfate) thereby consuming ATP. The APS is then reduced by the enzyme APS reductase to form sulfite (SO2− 3 ...
*  SLC35B2
Adenosine 3'-phospho 5'-phosphosulfate transporter 1 is a protein that in humans is encoded by the SLC35B2 gene. Solute carrier ... 2003). "Molecular cloning and identification of 3'-phosphoadenosine 5'-phosphosulfate transporter". J. Biol. Chem. 278 (28): ...
*  Dissimilatory sulfate reduction
... occurs in three steps: Conversion (activation) of sulfate to Adenosine 5'-phosphosulfate (APS) ...
*  APS
... known as Aps until 1904 Adenosine 5'-phosphosulfate, a metabolic precursor to 3'-phosphoadenosine-5'-phosphosulfate (PAPS) ...
*  Phosphoadenylyl-sulfate reductase (thioredoxin)
... adenosine 3',5'-bisphosphate,sulfite:oxidized-thioredoxin, and oxidoreductase (3'-phosphoadenosine-5'-phosphosulfate-forming). ... adenosine 3'-phosphate 5'-phosphosulfate reductase, 3'-phosphoadenylylsulfate reductase, thioredoxin:3'-phospho-adenylylsulfate ... The systematic name of this enzyme class is adenosine 3',5'-bisphosphate,sulfite:thioredoxin-disulfide oxidoreductase (3'- ... phosphoadenosine-5'-phosphosulfate-forming). Other names in common use include PAPS reductase, thioredoxin-dependent, PAPS ...
*  GAL3ST1
1996). "Purification and characterization of 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase from human renal ... a galactosylceramide to adenosine 3',5'-bisphosphate + galactosylceramide sulfate. Activity of this sulfotransferase is ... 1977). "Age-dependent modulation of 3'-phosphoadenosine-5'-phosphosulfate-galactosylceramide sulfotransferase by lipids ...
Adenosine deaminase conjugated with polyethylene glycol synonyms, adenosine deaminase conjugated with polyethylene glycol...  Adenosine deaminase conjugated with polyethylene glycol synonyms, adenosine deaminase conjugated with polyethylene glycol...
Antonyms for adenosine deaminase conjugated with polyethylene glycol. 2 words related to adenosine: biochemistry, nucleoside. ... What are synonyms for adenosine deaminase conjugated with polyethylene glycol? ... Synonyms for adenosine deaminase conjugated with polyethylene glycol in Free Thesaurus. ... Adenosine 5'-Phosphosulfate. *Adenosine 5'-Phosphosulfate. *adenosine 5'-phosphosulfate kinase. *adenosine 5'-triphosphate ...
more infohttps://www.freethesaurus.com/adenosine+deaminase+conjugated+with+polyethylene+glycol
Plant Adenosine 5-Phosphosulfate Reductase Is a Novel Iron-Sulfur Protein - RERO DOC  Plant Adenosine 5'-Phosphosulfate Reductase Is a Novel Iron-Sulfur Protein - RERO DOC
... phosphosulfate reductase (APR) catalyzes the two-electron reduction of adenosine 5'-phosphosulfate to sulfite and AMP, which ... Thus, plant assimilatory APR represents a novel type of adenosine 5'-phosphosulfate reductase with a [4Fe-4S] center as the ... sole cofactor, which is clearly different from the dissimilatory adenosine 5'-phosphosulfate reductases found in sulfate ... Adenosine 5'-phosphosulfate reductase (APR) catalyzes the two-electron reduction of adenosine 5'-phosphosulfate to sulfite and ...
more infohttp://doc.rero.ch/record/6270
Slc35b2 - Adenosine 3-phospho 5-phosphosulfate transporter 1 - Mus musculus (Mouse) - Slc35b2 gene & protein  Slc35b2 - Adenosine 3'-phospho 5'-phosphosulfate transporter 1 - Mus musculus (Mouse) - Slc35b2 gene & protein
... phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl donor for sulfation events that take place in the ... Adenosine 3'-phospho 5'-phosphosulfate transporter 1Add BLAST. 431. Proteomic databases. PaxDb, a database of protein abundance ... sp,Q91ZN5,S35B2_MOUSE Adenosine 3'-phospho 5'-phosphosulfate transporter 1 OS=Mus musculus OX=10090 GN=Slc35b2 PE=1 SV=1 ... Mediates the transport of adenosine 3'-phospho 5'-phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl ...
more infohttps://www.uniprot.org/uniprot/Q91ZN5
Adenosine 3ʹ-Phosphate 5ʹ-Phosphosulfate, Tetralithium Salt CAS 102029-54-9 | 118410  Adenosine 3ʹ-Phosphate 5ʹ-Phosphosulfate, Tetralithium Salt CAS 102029-54-9 | 118410
Adenosine 3ʹ-Phosphate 5ʹ-Phosphosulfate, Tetralithium Salt CAS 102029-54-9 - Find MSDS or SDS, a COA, data sheets and more ... Adenosine 3ʹ-Phosphate 5ʹ-Phosphosulfate, Tetralithium Salt. 118410 Adenosine 3ʹ-Phosphate 5ʹ-Phosphosulfate, Tetralithium Salt ...
more infohttp://www.merckmillipore.com/IE/en/product/Adenosine-3-Phosphate-5-Phosphosulfate-Tetralithium-Salt,EMD_BIO-118410
Purification of the Golgi adenosine 3-phosphate 5-phosphosulfate transporter, a homodimer within the membrane. - Semantic...  Purification of the Golgi adenosine 3'-phosphate 5'-phosphosulfate transporter, a homodimer within the membrane. - Semantic...
Adenosine 3'-phosphate 5'-phosphosulfate (PAPS), the sulfate donor in these reactions, must be transported from the cytosol, ... transporter by a combination of conventional and affinity chromatography as well as photoaffinity radiolabeling with adenosine ... Disruption of adenosine-5'-phosphosulfate kinase in Arabidopsis reduces levels of sulfated secondary metabolites.. *Sarah G. ... Adenosine 3'-phosphate 5'-phosphosulfate (PAPS), the sulfate donor in these reactions, must be transported from the cytosol, ...
more infohttps://www.semanticscholar.org/paper/Purification-of-the-Golgi-adenosine-3%27-phosphate-a-Mandon-Milla/c8d7cd9d24ae98cc2f312fe2e8b96fac38991849
Reaction Mechanism of the Iron−Sulfur Flavoenzyme Adenosine-5-Phosphosulfate Reductase Based on the Structural...  Reaction Mechanism of the Iron−Sulfur Flavoenzyme Adenosine-5'-Phosphosulfate Reductase Based on the Structural...
The iron-sulfur flavoenzyme adenosine-5'-phosphosulfate (APS) reductase catalyzes a key reaction of the global sulfur cycle by ... The iron-sulfur flavoenzyme adenosine-5'-phosphosulfate (APS) reductase catalyzes a key reaction of the global sulfur cycle by ... Reaction Mechanism of the Iron−Sulfur Flavoenzyme Adenosine-5'-Phosphosulfate Reductase Based on the Structural ... Reaction Mechanism of the Iron−Sulfur Flavoenzyme Adenosine-5'-Phosphosulfate Reductase Based on the Structural ...
more infohttps://kops.uni-konstanz.de/handle/123456789/37755
Activity and stability of recombinant bifunctional rearranged and monofunctional domains of ATP-sulfurylase and adenosine 5...  Activity and stability of recombinant bifunctional rearranged and monofunctional domains of ATP-sulfurylase and adenosine 5'...
Activity and stability of recombinant bifunctional rearranged and monofunctional domains of ATP-sulfurylase and adenosine 5'- ... Murine adenosine 3'-phosphate 5'-phosphosulfate (PAPS) synthetase consists of a COOH-terminal ATP-sulfurylase domain covalently ... linked through a nonhomologous intervening sequence to an NH2-terminal adenosine 5'-phosphosulfate (APS) kinase domain forming ... Activity and stability of recombinant bifunctional rearranged and monofunctional domains of ATP-sulfurylase and adenosine 5'- ...
more infohttps://scholars.duke.edu/display/pub1116126
NAVER Academic | A new assay for ATP sulfurylase based on differential solubility of the sodium salts of adenosine 5′...  NAVER Academic | A new assay for ATP sulfurylase based on differential solubility of the sodium salts of adenosine 5′...
... leaving the product adenosine 5′-phosphosulfate (APS) in solution with an 80% yield. This separation procedure, coupled with ... phosphosulfate (PAPS) under the assay conditions adopted, so that the 35S remaining in solution after ethanol precipitation of ... A new assay for ATP sulfurylase based on differential solubility of the sodium salts of adenosine 5′-phosphosulfate and sulfate ... leaving the product adenosine 5′-phosphosulfate (APS) in solution with an 80% yield. This separation procedure, coupled with ...
more infohttps://academic.naver.com/article.naver?doc_id=68730284
SLC35B2 Gene - GeneCards | S35B2 Protein | S35B2 Antibody  SLC35B2 Gene - GeneCards | S35B2 Protein | S35B2 Antibody
Adenosine 3-phospho 5-phosphosulfate transporter 1 Protein Accession:. Q8TB61. Secondary Accessions: *B4DDM2 ... 3-phosphoadenosine 5-phosphosulfate transport. IDA. 12716889. GO:0050428. 3-phosphoadenosine 5-phosphosulfate biosynthetic ... Mediates the transport of adenosine 3-phospho 5-phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl ... Mediates the transport of adenosine 3-phospho 5-phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl ...
more infohttp://www.genecards.org/cgi-bin/carddisp.pl?gene=SLC35B2
Insect haptoelectrical stimulation of Venus flytrap triggers exocytosis in gland cells | PNAS  Insect haptoelectrical stimulation of Venus flytrap triggers exocytosis in gland cells | PNAS
APS1-4, ATP sulfurylases 1-4; APR1-4, adenosine phosphosulfate reductases 1-4; GSH1 & 2, GSH synthetase 1 & 2; OASTL, O- ... 2010) Sulphur flux through the sulphate assimilation pathway is differently controlled by adenosine 5′-phosphosulphate ... adenosine 5′-phosphosulfate (APS)] via a well-known enzymatic pathway (Fig. S4). Gene expression analysis based on RNA- ...
more infohttps://www.pnas.org/content/114/18/4822
Chloroplasts in plant immunity | Philosophical Transactions of the Royal Society B: Biological Sciences  Chloroplasts in plant immunity | Philosophical Transactions of the Royal Society B: Biological Sciences
APS, adenosine 5′-phosphosulphate; ATPS, ATP sulphurylase; APK, APS kinase; APS, adenosine phosphosulphate; Ind, indole; Glc, ... Biosynthesis and sulfonylation of GLS requires glutathione (GSH) and the sulfur donor 5′-phosphosulphate (PAPS). The reaction ... phosphosulfate to the cytosol. Plant Cell 24, 4187-4204. (doi:10.1105/tpc.112.101964). ... phosphosulphate (PAPS), whose by-product phosphoadenosine 5′-phosphate (PAP) has been shown to mediate retrograde signals from ...
more infohttp://rstb.royalsocietypublishing.org/content/369/1640/20130235
PAPSS1 Gene - GeneCards | PAPS1 Protein | PAPS1 Antibody  PAPSS1 Gene - GeneCards | PAPS1 Protein | PAPS1 Antibody
Phosphosulfate Synthase 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human ... The first step is the transfer of a sulfate group to ATP to yield adenosine 5-phosphosulfate (APS), and the second step is the ... The first step is the transfer of a sulfate group to ATP to yield adenosine 5-phosphosulfate (APS), and the second step is the ... Structural mechanism for substrate inhibition of the adenosine 5'-phosphosulfate kinase domain of human 3'-phosphoadenosine 5'- ...
more infohttps://www.genecards.org/cgi-bin/carddisp.pl?gene=PAPSS1
Adenylyl-sulfate reductase (thioredoxin) - Wikipedia  Adenylyl-sulfate reductase (thioredoxin) - Wikipedia
Abola AP, Willits MG, Wang RC, Long SR (1999). "Reduction of Adenosine-5′-Phosphosulfate Instead of 3′-Phosphoadenosine-5′- ... The systematic name of this enzyme class is AMP,sulfite:thioredoxin-disulfide oxidoreductase (adenosine-5'-phosphosulfate- ... Williams SJ, Senaratne RH, Mougous JD, Riley LW, Bertozzi CR (2002). "5'-adenosinephosphosulfate lies at a metabolic branch ... Phosphosulfate in Cysteine Biosynthesis by Rhizobium meliloti and Other Members of the Family Rhizobiaceae". J. Bacteriol. 181 ...
more infohttps://en.wikipedia.org/wiki/Adenylyl-sulfate_reductase_(thioredoxin)
EC 1.7 and EC 1.8  EC 1.7 and EC 1.8
Other name(s): adenosine phosphosulfate reductase; adenosine 5'-phosphosulfate reductase; APS-reductase; APS reductase; AMP, ... phosphosulfate-forming). Systematic name: AMP,sulfite:acceptor oxidoreductase (adenosine-5'-phosphosulfate-forming). Comments: ... Systematic name: AMP,sulfite:glutathione-disulfide oxidoreductase (adenosine-5'-phosphosulfate-forming). Comments: This enzyme ... 2. Abola, A.P., Willits, M.G., Wang, R.C. and Long, S.R. Reduction of adenosine-5'-phosphosulfate instead of 3'- ...
more infohttps://www.qmul.ac.uk/sbcs/iubmb/enzyme/EC1/07p.html
Frontiers | Reconstruction of the Metabolic Potential of Acidophilic Sideroxydans Strains from the Metagenome of an...  Frontiers | Reconstruction of the Metabolic Potential of Acidophilic Sideroxydans Strains from the Metagenome of an...
This involves sulfate activation by a sulfate adenylyltransferase to adenosine-phosphosulfate (APS) and subsequent reduction to ... Sulfate is predicted to be subsequently assimilated via the adenosine phosphosulfate (APS) pathway. ...
more infohttps://www.frontiersin.org/articles/10.3389/fmicb.2016.02082/full
NeMeSys: a biological resource for narrowing the gap between sequence and function in the human pathogen Neisseria meningitidis...  NeMeSys: a biological resource for narrowing the gap between sequence and function in the human pathogen Neisseria meningitidis...
Abola AP, Willits MG, Wang RC, Long SR: Reduction of adenosine-5'-phosphosulfate instead of 3'-phosphoadenosine-5'- ... CysD and CysN might transform sulfate into adenosine 5'-phosphosulfate (APS). Usually, APS is phosphorylated into ... phosphosulfate in cysteine biosynthesis by Rhizobium meliloti and other members of the family Rhizobiaceae. J Bacteriol. 1999, ... phosphoadenosine-5'-phosphosulfate (PAPS), which is then reduced into sulfite by a PAPS reductase, but there is no gene ...
more infohttps://genomebiology.biomedcentral.com/articles/10.1186/gb-2009-10-10-r110
Vaccine efficacy of an attenuated but persistent Mycobacterium tuberculosis cysH mutant.  - PubMed - NCBI  Vaccine efficacy of an attenuated but persistent Mycobacterium tuberculosis cysH mutant. - PubMed - NCBI
This study reports the vaccine potential of an attenuated 5'-adenosine phosphosulfate reductase mutant (DeltacysH) of M. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/17374883?dopt=Abstract
The Journal of General and Applied Microbiology  The Journal of General and Applied Microbiology
... and adenosine phosphosulfate reductase were also determined. Although cells of strains MF and MY were clearly distinguished ...
more infohttps://www.jstage.jst.go.jp/browse/jgam/28/1/_contents
Adenylyl-sulfate reductase - Wikipedia  Adenylyl-sulfate reductase - Wikipedia
... phosphosulfate-forming). Other names in common use include adenosine phosphosulfate reductase, adenosine 5'-phosphosulfate ... reductase, APS-reductase, APS reductase, AMP, sulfite:(acceptor) oxidoreductase, and (adenosine-5'-phosphosulfate-forming). ... The systematic name of this enzyme class is AMP,sulfite:acceptor oxidoreductase (adenosine-5'- ...
more infohttps://en.wikipedia.org/wiki/Adenylyl-sulfate_reductase
Frontiers | Sulfur Metabolisms in Epsilon- and Gamma-Proteobacteria in Deep-Sea Hydrothermal Fields | Microbiology  Frontiers | Sulfur Metabolisms in Epsilon- and Gamma-Proteobacteria in Deep-Sea Hydrothermal Fields | Microbiology
Psr, polysulfide reductase; Sox, Sox multienzyme system; Dsr, dissimilatory sulfite reductase; Apr, adenosine 5′-phosphosulfate ...
more infohttps://www.frontiersin.org/articles/10.3389/fmicb.2011.00192/full
  • Sulfate is activated at the expense of two ATP equivalents through the activity of sulfate adenylyltransferase ( equation 1 ), producing adenosine phosphosulfate (APS), which increases the redox potential of the APS/HSO 3 − couple to −60 mV ( 5 , 6 ), allowing reduction by electrons from H 2 or organic electron donors. (asm.org)