A negative regulator of beta-catenin signaling which is mutant in ADENOMATOUS POLYPOSIS COLI and GARDNER SYNDROME.
A polyposis syndrome due to an autosomal dominant mutation of the APC genes (GENES, APC) on CHROMOSOME 5. The syndrome is characterized by the development of hundreds of ADENOMATOUS POLYPS in the COLON and RECTUM of affected individuals by early adulthood.
Tumor suppressor genes located in the 5q21 region on the long arm of human chromosome 5. The mutation of these genes is associated with familial adenomatous polyposis (ADENOMATOUS POLYPOSIS COLI) and GARDNER SYNDROME, as well as some sporadic colorectal cancers.
A multi-functional catenin that participates in CELL ADHESION and nuclear signaling. Beta catenin binds CADHERINS and helps link their cytoplasmic tails to the ACTIN in the CYTOSKELETON via ALPHA CATENIN. It also serves as a transcriptional co-activator and downstream component of WNT PROTEIN-mediated SIGNAL TRANSDUCTION PATHWAYS.
Major constituent of the cytoskeleton found in the cytoplasm of eukaryotic cells. They form a flexible framework for the cell, provide attachment points for organelles and formed bodies, and make communication between parts of the cell possible.
A glycogen synthase kinase that was originally described as a key enzyme involved in glycogen metabolism. It regulates a diverse array of functions such as CELL DIVISION, microtubule function and APOPTOSIS.
Slender, cylindrical filaments found in the cytoskeleton of plant and animal cells. They are composed of the protein TUBULIN and are influenced by TUBULIN MODULATORS.
Discrete abnormal tissue masses that protrude into the lumen of the INTESTINE. A polyp is attached to the intestinal wall either by a stalk, pedunculus, or by a broad base.
A scaffolding protein that is a critical component of the axin signaling complex which binds to ADENOMATOUS POLYPOSIS COLI PROTEIN; GLYCOGEN SYNTHASE KINASE 3; and CASEIN KINASE I.
Benign neoplasms derived from glandular epithelium. (From Stedman, 25th ed)
The growth of INTESTINAL POLYPS. Growth processes include neoplastic (ADENOMA and CARCINOMA) and non-neoplastic (hyperplastic, mucosal, inflammatory, and other polyps).
Proteins obtained from ESCHERICHIA COLI.
A childhood counterpart of abdominal or extra-abdominal desmoid tumors, characterized by firm subcutaneous nodules that grow rapidly in any part of the body but do not metastasize. The adult form of abdominal fibromatosis is FIBROMATOSIS, ABDOMINAL. (Stedman, 25th ed)
A benign epithelial tumor with a glandular organization.
Tumors or cancer of the INTESTINES.
Tumors or cancer of the COLON or the RECTUM or both. Risk factors for colorectal cancer include chronic ULCERATIVE COLITIS; FAMILIAL POLYPOSIS COLI; exposure to ASBESTOS; and irradiation of the CERVIX UTERI.
A variant of ADENOMATOUS POLYPOSIS COLI caused by mutation in the APC gene (GENES, APC) on CHROMOSOME 5. It is characterized by not only the presence of multiple colonic polyposis but also extracolonic ADENOMATOUS POLYPS in the UPPER GASTROINTESTINAL TRACT; the EYE; the SKIN; the SKULL; and the FACIAL BONES; as well as malignancy in organs other than the GI tract.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
Tumors or cancer of the COLON.
Discrete abnormal tissue masses that protrude into the lumen of the DIGESTIVE TRACT or the RESPIRATORY TRACT. Polyps can be spheroidal, hemispheroidal, or irregular mound-shaped structures attached to the MUCOUS MEMBRANE of the lumen wall either by a stalk, pedunculus, or by a broad base.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Wnt proteins are a large family of secreted glycoproteins that play essential roles in EMBRYONIC AND FETAL DEVELOPMENT, and tissue maintenance. They bind to FRIZZLED RECEPTORS and act as PARACRINE PROTEIN FACTORS to initiate a variety of SIGNAL TRANSDUCTION PATHWAYS. The canonical Wnt signaling pathway stabilizes the transcriptional coactivator BETA CATENIN.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A relatively large mass of unusually firm scarlike connective tissue resulting from active participation of fibroblasts, occurring most frequently in the abdominal muscles of women who have borne children. The fibroblasts infiltrate surrounding muscle and fascia. (Stedman, 25th ed)
One of the two pairs of human chromosomes in the group B class (CHROMOSOMES, HUMAN, 4-5).
Any detectable and heritable alteration in the lineage of germ cells. Mutations in these cells (i.e., "generative" cells ancestral to the gametes) are transmitted to progeny while those in somatic cells are not.
Tumors or cancer of the DUODENUM.
Discrete tissue masses that protrude into the lumen of the COLON. These POLYPS are connected to the wall of the colon either by a stalk, pedunculus, or by a broad base.
A family of DNA-binding proteins that are primarily expressed in T-LYMPHOCYTES. They interact with BETA CATENIN and serve as transcriptional activators and repressors in a variety of developmental processes.
A sulfinylindene derivative prodrug whose sulfinyl moiety is converted in vivo to an active NSAID analgesic. Specifically, the prodrug is converted by liver enzymes to a sulfide which is excreted in the bile and then reabsorbed from the intestine. This helps to maintain constant blood levels with reduced gastrointestinal side effects.
A surgical procedure involving the excision of the COLON and RECTUM and the formation of an ILEOANAL RESERVOIR (pouch). In patients with intestinal diseases, such as ulcerative colitis, this procedure avoids the need for an OSTOMY by allowing for transanal defecation.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
Genes that inhibit expression of the tumorigenic phenotype. They are normally involved in holding cellular growth in check. When tumor suppressor genes are inactivated or lost, a barrier to normal proliferation is removed and unregulated growth is possible.

Axin prevents Wnt-3a-induced accumulation of beta-catenin. (1/711)

When Axin, a negative regulator of the Wnt signaling pathway, was expressed in COS cells, it coeluted with glycogen synthase kinase-3beta (GSK-3beta), beta-catenin, and adenomatous polyposis coli protein (APC) in a high molecular weight fraction on gel filtration column chromatography. In this fraction, GSK-3beta, beta-catenin, and APC were co-precipitated with Axin. Although beta-catenin was detected in the high molecular weight fraction in L cells on gel filtration column chromatography, addition of conditioned medium expressing Wnt-3a to the cells increased beta-catenin in the low molecular weight fraction. However, Wnt-3a-dependent accumulation of beta-catenin was greatly inhibited in L cells stably expressing Axin. Axin also suppressed Wnt-3a-dependent activation of Tcf-4 which binds to beta-catenin and acts as a transcription factor. These results suggest that Axin forms a complex with GSK-3beta, beta-catenin, and APC, resulting in the stimulation of the degradation of beta-catenin and that Wnt-3a induces the dissociation of beta-catenin from the Axin complex and accumulates beta-catenin.  (+info)

EB1, a protein which interacts with the APC tumour suppressor, is associated with the microtubule cytoskeleton throughout the cell cycle. (2/711)

The characteristics of the adenomatous polyposis coli (APC) associated protein EB1 were examined in mammalian cells. By immunocytochemistry EB1 was shown to be closely associated with the microtubule cytoskeleton throughout the cell cycle. In interphase cells EB1 was associated with microtubules along their full length but was often particularly concentrated at their tips. During early mitosis, EB1 was localized to separating centrosomes and associated microtubules, while at metaphase it was associated with the spindle poles and associated microtubules. During cytokinesis EB1 was strongly associated with the midbody microtubules. Treatment with nocodazole caused a diffuse redistribution of EB1 immunoreactivity, whereas treatment with cytochalasin D had no effect. Interestingly, treatment with taxol abolished the EB1 association with microtubules. In nocodazole washout experiments EB1 rapidly became associated with the centrosome and repolymerizing microtubules. In taxol wash-out experiments EB1 rapidly re-associated with the microtubule cytoskeleton, resembling untreated control cells within 10 min. Immunostaining of SW480 cells, which contain truncated APC incapable of interaction with EB1, showed that the association of EB1 with microtubules throughout the cell cycle was not dependent upon an interaction with APC. These results suggest a role for EB1 in the control of microtubule dynamics in mammalian cells.  (+info)

Analysis of masked mutations in familial adenomatous polyposis. (3/711)

Familial adenomatous polyposis (FAP) is an autosomal-dominant disease characterized by the development of hundreds of adenomatous polyps of the colorectum. Approximately 80% of FAP patients can be shown to have truncating mutations of the APC gene. To determine the cause of FAP in the other 20% of patients, MAMA (monoallelic mutation analysis) was used to independently examine the status of each of the two APC alleles. Seven of nine patients analyzed were found to have significantly reduced expression from one of their two alleles whereas two patients were found to have full-length expression from both alleles. We conclude that more than 95% of patients with FAP have inactivating mutations in APC and that a combination of MAMA and standard genetic tests will identify APC abnormalities in the vast majority of such patients. That no APC expression from the mutant allele is found in some FAP patients argues strongly against the requirement for dominant negative effects of APC mutations. The results also suggest that there may be at least one additional gene, besides APC, that can give rise to FAP.  (+info)

Administration of an unconjugated bile acid increases duodenal tumors in a murine model of familial adenomatous polyposis. (4/711)

Intestinal carcinogenesis involves the successive accumulation of multiple genetic defects until cellular transformation to an invasive phenotype occurs. This process is modulated by many epigenetic factors. Unconjugated bile acids are tumor promoters whose presence in intestinal tissues is regulated by dietary factors. We studied the role of the unconjugated bile acid, chenodeoxycholate, in an animal model of familial adenomatous polyposis. Mice susceptible to intestinal tumors as a result of a germline mutation in Apc (Min/+ mice) were given a 10 week dietary treatment with 0.5% chenodeoxycholate. Following this, the mice were examined to determine tumor number, enterocyte proliferation, apoptosis and beta-catenin expression. Intestinal tissue prostaglandin E2 (PGE2) levels were also assessed. Administration of chenodeoxycholate in the diet increased duodenal tumor number in Min/+ mice. Promotion of duodenal tumor formation was accompanied by increased beta-catenin expression in duodenal cells, as well as increased PGE2 in duodenal tissue. These data suggest that unconjugated bile acids contribute to periampullary tumor formation in the setting of an Apc mutation.  (+info)

Negative regulation of Wingless signaling by D-axin, a Drosophila homolog of axin. (5/711)

Wnt/Wingless directs many cell fates during development. Wnt/Wingless signaling increases the amount of beta-catenin/Armadillo, which in turn activates gene transcription. Here the Drosophila protein D-Axin was shown to interact with Armadillo and D-APC. Mutation of d-axin resulted in the accumulation of cytoplasmic Armadillo and one of the Wingless target gene products, Distal-less. Ectopic expression of d-axin inhibited Wingless signaling. Hence, D-Axin negatively regulates Wingless signaling by down-regulating the level of Armadillo. These results establish the importance of the Axin family of proteins in Wnt/Wingless signaling in Drosophila.  (+info)

Hereditary desmoid disease in a family with a germline Alu I repeat mutation of the APC gene. (6/711)

Two families with autosomal dominantly inherited desmoid tumors have recently been shown to have germline mutations at the 3' end of the APC gene. We subsequently identified an Amish family with autosomal dominantly inherited desmoid tumors. Genetic analysis performed on one family member, a 47-year-old man with multiple desmoid tumors and no colon polyps, revealed a protein truncating mutation in the middle of the APC gene. The truncating mutation is the result of a 337-bp insertion of an Alu I sequence into codon 1526 of the APC gene. The presence of a poly(A) tail at the 3' end of the insertion suggests that the Alu I sequence was inserted by a retrotranspositional event. Germline insertions of Alu I sequences have occasionally been reported to cause other genetic diseases including type I neurofibromatosis, hereditary site-specific breast cancer (BRCA2), and hemophilia B. However, this is the first report of a germline mutation of the APC gene resulting from an Alu I insertion.  (+info)

E-cadherin binding prevents beta-catenin nuclear localization and beta-catenin/LEF-1-mediated transactivation. (7/711)

Beta-catenin is a multifunctional protein found in three cell compartments: the plasma membrane, the cytoplasm and the nucleus. The cell has developed elaborate ways of regulating the level and localization of beta-catenin to assure its specific function in each compartment. One aspect of this regulation is inherent in the structural organization of beta-catenin itself; most of its protein-interacting motifs overlap so that interaction with one partner can block binding of another at the same time. Using recombinant proteins, we found that E-cadherin and lymphocyte-enhancer factor-1 (LEF-1) form mutually exclusive complexes with beta-catenin; the association of beta-catenin with LEF-1 was competed out by the E-cadherin cytoplasmic domain. Similarly, LEF-1 and adenomatous polyposis coli (APC) formed separate, mutually exclusive complexes with beta-catenin. In Wnt-1-transfected C57MG cells, free beta-catenin accumulated and was able to associate with LEF-1. The absence of E-cadherin in E-cadherin-/- embryonic stem (ES) cells also led to an accumulation of free beta-catenin and its association with LEF-1, thereby mimicking Wnt signaling. beta-catenin/LEF-1-mediated transactivation in these cells was antagonized by transient expression of wild-type E-cadherin, but not of E-cadherin lacking the beta-catenin binding site. The potent ability of E-cadherin to recruit beta-catenin to the cell membrane and prevent its nuclear localization and transactivation was also demonstrated using SW480 colon carcinoma cells.  (+info)

Regulation of beta-catenin signaling by the B56 subunit of protein phosphatase 2A. (8/711)

Dysregulation of Wnt-beta-catenin signaling disrupts axis formation in vertebrate embryos and underlies multiple human malignancies. The adenomatous polyposis coli (APC) protein, axin, and glycogen synthase kinase 3beta form a Wnt-regulated signaling complex that mediates the phosphorylation-dependent degradation of beta-catenin. A protein phosphatase 2A (PP2A) regulatory subunit, B56, interacted with APC in the yeast two-hybrid system. Expression of B56 reduced the abundance of beta-catenin and inhibited transcription of beta-catenin target genes in mammalian cells and Xenopus embryo explants. The B56-dependent decrease in beta-catenin was blocked by oncogenic mutations in beta-catenin or APC, and by proteasome inhibitors. B56 may direct PP2A to dephosphorylate specific components of the APC-dependent signaling complex and thereby inhibit Wnt signaling.  (+info)

Adenomatous Polyposis Coli Protein: A negative regulator of beta-catenin signaling which is mutant in ADENOMATOUS POLYPOSIS COLI and GARDNER SYNDROME.
Research Grants, Research Topics, Publications, Genomes and Genes, Species, Scientific Experts about adenomatous polyposis coli protein
Our findings implicate the APC tumor suppressor gene in the process of axial patterning in Xenopus. Since induction of the dorsoanterior axis involves the activities of embryonic signaling centers (or organizers), these findings demonstrate that APC has signal transduction activity. APC seems to act in the same signaling pathway as β-catenin. They have similar characteristics, including induction of the homeobox protein Siamois. Furthermore, APC signaling is strongly dependent on the availability of a free cytosolic pool of β-catenin, which by itself has axis-inducing activity. Moreover, APC or APC/β-catenin complexes seem to have direct positive signaling activity, since APC does not act indirectly simply by binding up β-catenin or by changing the levels of β-catenin. We propose, therefore, that axis induction by APC is due to its role in a signal transduction process in which β-catenin has been strongly implicated.. The induction of a dorsoanterior axis in Xenopus results from localized ...
A unique feature of colon cancer is that truncation mutations in the APC (adenomatous polyposis coli) gene are common to most tumours. The high penetrance of APC mutations, especially in gut epithelium, supports the idea that APC may be involved in a number of the processes that govern the normal maintenance of this tissue: differentiation, migration, proliferation and apoptosis. Indeed, APC is involved in the regulation of β-catenin and it also is an important regulator of the cytoskeleton. Thus mutations in APC lead to the accumulation of β-catenin, which causes changes in differentiation, and they also produce changes in cytoskeletal organization, which results in altered cell migration and disrupted mitotic spindles. The function of APC in cytoskeletal organization is related to its effect on microtubules and F-actin. Depleting APC from cultured cells leads to changes in cytoskeletal organization. In addition, N-terminal fragments of APC, like those commonly found in tumours, compromise ...
We show that expression of stabilized β-catenin decreased neurite initiation and elongation in NGF-treated PC12 cells (Fig. 5). Several mechanisms could explain how stabilized β-catenin inhibits neurite outgrowth in PC12 cells. When β-catenin is stabilized by Wnt signals it can promote cadherin-mediated cell-cell adhesion (Hinck et al., 1994) in addition to Tcf/Lef-mediated transcription. Experiments expressing stabilized β-catenin in whole animals or in neuronal cultures directly contacting glial cells may mask the role of β-catenin in the APC complex with its role in adhesion (Yu and Malenka, 2004; Loureiro et al., 2001; Elul et al., 2003). Previous work on the role of β-catenin in branching of axons and dendrites uses neurons in direct cell-cell contact with a glial feeder layer, and β-catenin is thought to require N-cadherin for this effect (Yu and Malenka, 2003; Yu and Malenka, 2004). PC12 cells do not form distinct axons and dendrites (Greene et al., 1998) and, if treated with NGF ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
In the present study, our comprehensive behavioral test battery revealed that Apc1638T/1638T mice shows impaired learning and memory, increased locomotor activity, mildly increased depression-like behavior, reduced anxiety-like behavior and mildly decreased social interaction behavior. In the hippocampal CA1 region of Apc1638T/1638T mice, the dendritic spine density and size are reduced, the PSD was smaller, and LTP was impaired. Taken together, our findings provide the first direct evidence of neuropsychological roles for the C-terminus of Apc tumor suppressor.. Apc1638T/1638T mice showed hyperactivity, impaired memory, increased depression-like behavior, and decreased social interaction. These behavioral characteristics are often linked to symptoms of schizophrenia (see Supplementary Table 11 in [15]) and observed in many animal models of schizophrenia [15-19]. In particular, Apc1638T/1638T mice showed a marked deficit in the performance of the working memory task. Impaired working memory is ...
3.0.CO;2-R. PMID 8806074. Sheikh F, Chen Y, Chen Y, Liang X, Hirschy A, Stenbit AE, Gu Y, Dalton ND, Yajima T, Lu Y, Knowlton KU, Peterson KL, Perriard JC, Chen J (Sep 2006). alpha-E-catenin inactivation disrupts the cardiomyocyte adherens junction, resulting in cardiomyopathy and susceptibility to wall rupture. Circulation. 114 (10): 1046-55. doi:10.1161/CIRCULATIONAHA.106.634469. PMID 16923756. Su LK, Vogelstein B, Kinzler KW (December 1993). Association of the APC tumor suppressor protein with catenins. Science. 262 (5140): 1734-7. doi:10.1126/science.8259519. PMID 8259519. Daniel JM, Reynolds AB (September 1995). The tyrosine kinase substrate p120cas binds directly to E-cadherin but not to the adenomatous polyposis coli protein or alpha-catenin. Mol. Cell. Biol. 15 (9): 4819-24. doi:10.1128/mcb.15.9.4819. PMC 230726 . PMID 7651399. Oyama T, Kanai Y, Ochiai A, Akimoto S, Oda T, Yanagihara K, Nagafuchi A, Tsukita S, Shibamoto S, Ito F (December 1994). A truncated beta-catenin disrupts ...
Truncating mutations in adenomatous polyposis coli (Apc) are strongly linked to colorectal cancers. APC is a negative regulator of the Wnt pathway and constitutive Wnt activation mediated by enhanced Wnt-β-catenin target gene activation is believed to be the predominant mechanism responsible for Apc mutant phenotypes. However, recent evidence suggests that additional downstream effectors contribute to Apc mutant phenotypes. We previously identified a mechanism in cultured human cells by which APC, acting through glycogen synthase kinase-3 (GSK-3), suppresses mTORC1, a nutrient sensor that regulates cell growth and proliferation. We hypothesized that truncating Apc mutations should activate mTORC1 in vivo and that mTORC1 plays an important role in Apc mutant phenotypes. We find mTORC1 is strongly activated in apc mutant zebrafish and in intestinal polyps in Apc mutant mice. Furthermore, mTORC1 activation is essential downstream of APC as mTORC1 inhibition partially rescues Apc mutant phenotypes ...
Adenomatous polyposis coli (APC) also known as deleted in polyposis 2.5 (DP2.5) is a protein that in humans is encoded by the APC gene. The APC protein is a negative regulator that controls beta-catenin concentrations and interacts with E-cadherin, which are involved in cell adhesion. Mutations in the APC gene may result in colorectal cancer. APC is classified as a tumor suppressor gene. Tumor suppressor genes prevent the uncontrolled growth of cells that may result in cancerous tumors. The protein made by the APC gene plays a critical role in several cellular processes that determine whether a cell may develop into a tumor. The APC protein helps control how often a cell divides, how it attaches to other cells within a tissue, how the cell polarizes and the morphogenesis of the 3D structures, or whether a cell moves within or away from a tissue. This protein also helps ensure that the chromosome number in cells produced through cell division is correct. The APC protein accomplishes these tasks ...
Actin Cytoskeletal 43 kD protein forming the backbone of the cytoplasmic microfilament system and the thin filament system of muscle sacomeres. In humans, there are distinct muscle (4 alpha-skeletal, alpha-cardiac, alpha-smooth, gamma-smooth) and non-muscle (2 cytoskeletal beta-, gamma-) actin isoforms. Exists as globular actin monomer (G-actin) and microfilament polymer (F-actin). Adenomatous Polyposis Coli Protein A negative regulator of beta-catenin signaling. These gene is associated with familial adenomatous polyposis (FAP), an inherited disorder characterized by the development of myriad polyps in the colon. The gene is located on chromosome 5. [48] Amino acid One of the 20 building blocks of protein. The sequence of amino acids in a protein and, hence, the function of that protein are determined by the genetic code in the DNA. Amino acids are molecules that (in technical terms) contain a basic amino (NH2) group, an acidic carboxyl (COOH) group and a side chain attached to an alpha carbon ...
Actin Cytoskeletal 43 kD protein forming the backbone of the cytoplasmic microfilament system and the thin filament system of muscle sacomeres. In humans, there are distinct muscle (4 alpha-skeletal, alpha-cardiac, alpha-smooth, gamma-smooth) and non-muscle (2 cytoskeletal beta-, gamma-) actin isoforms. Exists as globular actin monomer (G-actin) and microfilament polymer (F-actin). Adenomatous Polyposis Coli Protein A negative regulator of beta-catenin signaling. These gene is associated with familial adenomatous polyposis (FAP), an inherited disorder characterized by the development of myriad polyps in the colon. The gene is located on chromosome 5. [47] Amino acid One of the 20 building blocks of protein. The sequence of amino acids in a protein and, hence, the function of that protein are determined by the genetic code in the DNA. Amino acids are molecules that (in technical terms) contain a basic amino (NH2) group, an acidic carboxyl (COOH) group and a side chain attached to an alpha carbon ...
Mutations of APC appear to initiate sporadic and inherited forms of human colorectal cancer. Although these mutations have been well characterized, little is known about the function of the APC gene product. Two cellular proteins that associate with APC were identified by nucleotide sequence analysis and peptide mapping as the E-cadherin-associated proteins alpha- and beta-catenin. A 27-residue fragment of APC containing a 15-amino acid repeat was sufficient for the interaction with the catenins. These results suggest an important link between tumor initiation and cell adhesion. ...
Despite the importance of cholinergic synapses for cognitive and autonomic functions, little is known about the mechanisms that direct their assembly during development. In a new study published in the June 2008 issue of Molecular and Cellular Neuroscience, researchers at Tufts University School of Medicine (TUSM), uncover mechanisms that direct cholinergic synapse assembly between neurons in vivo. We have identified the protein adenomatous polyposis coli (APC) as a key organizer of a multi-protein complex that is required for assembly of neuronal cholinergic synapses says corresponding author Michele H. Jacob, PhD, professor of neuroscience at TUSM and member of the neuroscience program faculty of the Sackler School of Graduate Biomedical Sciences. APC is expressed in all cell types and has multiple functions and binding partners. It is best known for its role in colorectal cancer. Our work defines a novel role for APC in neurons. We show that APC brings together several proteins at the ...
The trafficking of the adenomatous polyposis coli (APC) tumour suppressor protein in mammalian cells is a perennially controversial topic. Immunostaining evidence for an actin-associated APC localisation at intercellular junctions has been previously presented, though live imaging of mammalian junctional APC has not been documented. Using live imaging of transfected COS-7 cells we observed intercellular junction-associated pools of GFP-APC in addition to previously documented microtubule-associated GFP-APC and a variety of minor localisations. Although both microtubule and junction-associated populations could co-exist within individual cells, they differed in their subcellular location, dynamic behaviour and sensitivity to cytoskeletal poisons. GFP-APC deletion mutant analysis indicated that a protein truncated immediately after the APC armadillo repeat domain retained the ability to localise to adhesive membranes in transfected cells. Supporting this, we also observed junctional APC immunostaining in
Our results implied that the n‐NESs of APC truncations provide insufficient nuclear export activity for the reduction of TCF transcription. To confirm this experimentally, we carried out complementation assays of SW480 cells that lack endogenous APC function; this function can be restored if the cells are transfected with minimal APC fragments (Munemitsu et al., 1995). We thus compared the function of wild‐type APC with that of two APC mutants without n‐NESs (HC, the central third of APC; mAPC, full‐length APC with mutated n‐NESs) and of two mutants without c‐NESs (NAPC, a type I truncation; APCala, full‐length APC with mutated c‐NESs) (Figure 1). We examined the subcellular distributions of these constructs in transfected SW480 cells, and compared their function in reducing nuclear β‐catenin and TOPFLASH activity. Their expression levels were monitored by western blotting (Figure 5E).. HC is largely excluded from the nuclei of SW480 cells, correlating with low levels of ...
Beginning at Leu 1029, the APC‐rA peptide adopts a conformation strikingly different from that of XTcf3 or E‐cadherin (Figure 3B). The C‐terminal half of the peptide, from Leu1029 to Glu1034, bulges out of the β‐catenin groove, away from the paths of XTcf3 and E‐cadherin (Figures 2B and 3B). This difference in conformation is probably due to the presence of a lysine residue at amino acid 1030 of APC‐rA. In XTcf3 and E‐cadherin this position is occupied by an acidic residue (Glu24 of XTcf3, Glu682 of E‐cadherin), which forms a salt bridge with β‐catenin Lys312 to form the second charged button of the extended region (Figure 3B). The lysine at this position in APC‐rA probably causes charge repulsion with β‐catenin Lys312, leading to a deviation from the conformations of the other two ligands. The conformation of the backbone at Lys1030 suggests that the side chain of this residue is in the vicinity of β‐catenin Glu462 and several other acidic residues. Although APC‐rA ...
EB1 is a microtubule tip-associated protein that interacts with the APC tumour suppressor protein and components of the dynein/dynactin complex.
The (Adenomatous Polyposis Coli) APC protein normally builds a destruction complex with glycogen synthase kinase 3-alpha and or beta (GSK-3α/β) and axin via interactions with the 20 AA and SAMP repeats[citation needed]. This complex is then able to bind β-catenins in the cytoplasm, that have dissociated from adherens contacts between cells. With the help of casein kinase 1 (CK1), which carries out an initial phosphorylation of β-catenin, GSK-3β is able to phosphorylate β-catenin a second time. This targets β-catenin for ubiquitination and degradation by cellular proteasomes. This prevents it from translocating into the nucleus, where it acts as a transcription factor for proliferation genes. APC is also thought to be targeted to microtubules via the PDZ binding domain, stabilizing them[citation needed]. The deactivation of the APC protein can take place after certain chain reactions in the cytoplasm are started, e.g. through the Wnt signals that destroy the conformation of the ...
Catenin is a critical transducer of the Wnt transmission pathway and takes on an important part in many developmental and cellular processes. signaling pathway by STI-571 may be further explored as an important target for alternate/adjuvant treatments for any broader range of human being cancer. receptors, aswell as the determined co-receptors LRP5 and LRP6 lately, resulting in phosphorylation from the proteins. It then affiliates with Axin as well as the adenomatous polyposis coli (APC) tumor suppressor, avoiding GSK3b from phosphorylating -catenin. Unphosphorylated -catenin can be stabilized by escaping reputation by ubiquitin/proteosome complicated, and finally translocates towards the nucleus where it engages transcription elements LEF/TCF-4 to activate the manifestation of downstream focuses 1616113-45-1 supplier on, such as for example c-Myc and cyclin D1 [3,6-11]. The participation of -catenin signaling in tumorigenesis was initially founded in colorectal tumor, where in fact the ARHGAP26 ...
The protein encoded by this gene functions as a protein ubiquitin ligase and is a component of the multiprotein APC complex. The APC complex is a cyclin degradation system that governs exit from mitosis by targeting cell cycle proteins for degredation by the 26S proteasome. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein, and other APC complex proteins, contain a tetratricopeptide repeat (TPR) domain; a protein domain that is often involved in protein-protein interactions and the assembly of multiprotein complexes. Multiple alternatively spliced transcript variants, encoding distinct proteins, have been identified. [provided by RefSeq, Jan 2016 ...
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Kundu, Chanakya et al Adenomatous polyposis coli (APC) protein causes hypersensitivity of mouse embryonic fibroblast cell lines to DNA-alkylating agent methylmethane sulfonate through base excision repair pathway . Cancer Research 67.9 Supplement (2007): 1961. Web. 21 Feb. 2018. ...
Canonical WNT signalling plays a critical role in the regulation of ovarian development; mis-regulation of this key pathway in the adult ovary is associated with subfertility and tumourigenesis. The roles of Adenomatous polyposis coli 2 (APC2), a little-studied WNT signalling pathway regulator, in ovarian homeostasis, fertility and tumourigenesis have not previously been explored. Here, we demonstrate essential roles of APC2 in regulating ovarian WNT signalling and ovarian homeostasis. A detailed analysis of ovarian histology, gene expression, ovulation and hormone levels was carried out in 10 week old and in aged constitutive APC2-knockout (Apc2−/−) mice (mixed background). Statistical significance for qRT-PCR data was determined from 95% confidence intervals. Significance testing was performed using 2-tailed Students t-test, when 2 experimental cohorts were compared. When more were compared, ANOVA test was used, followed by a post-hoc test (LSD or Games-Howell). P-values of | 0.05 were considered
human AMER1 protein: regulates the distribution of the tumor suppressor APC between microtubules and the plasma membrane; amino acid sequence in first source
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Complete information for SAPCD2 gene (Protein Coding), Suppressor APC Domain Containing 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
TY - JOUR. T1 - A novel APC promoter 1B deletion shows a founder effect in Italian patients with classical familial adenomatous polyposis phenotype. AU - Marabelli, M.. AU - Gismondi, V.. AU - Ricci, M. T.. AU - Vetro, A.. AU - Khouzam, R. Abou. AU - Rea, V.. AU - Vitellaro, M.. AU - Zuffardi, O.. AU - Varesco, L.. AU - Ranzani, G. N.. N1 - LR: 20180201; CI: (c) 2017; JID: 9007329; 0 (APC protein, human); 0 (Adenomatous Polyposis Coli Protein); 2017/06/01 00:00 [received]; 2017/08/05 00:00 [revised]; 2017/08/07 00:00 [accepted]; 2017/08/10 06:00 [pubmed]; 2018/02/02 06:00 [medline]; 2017/08/10 06:00 [entrez]; ppublish. PY - 2017/12/1. Y1 - 2017/12/1. N2 - Familial adenomatous polyposis is a Mendelian syndrome in which germline loss-of-function mutations of APC are associated with multiple adenomatous polyps of the large bowel, a multiplicity of extracolonic features, and a high lifetime risk of colorectal cancer. Different APC germline mutations have been identified, including sequence changes, ...
The adenomatous polyposis coli (Apc) gene is mutated in familial adenomatous polyposis and in sporadic colorectal tumors. The Apc gene product (APC), basically a cytoplasmic protein, blocks cell cycle
AbstractPURPOSE:The aim of this study is to show that the diagnosis of attenuated adenomatous polyposis coli must be made with caution and certainly only after adequate colonic examination with dye-spray.METHODS:Four patients thought to have attenuated adenomatous polyposis coli on the basis of fami
Adenomatous Polyposis Coli; Polyposis Coli, Familial; Polyposis Syndrome, Familial. On-line free medical diagnosis assistant. Ranked list of possible diseases from either several symptoms or a full patient history. A similarity measure between symptoms and diseases is provided.
Aberrant methylation of the adenomatous polyposis coli (APC) gene promoter occurs in about 40% of breast tumours and has been correlated with reduced APC protein levels. To what extent epigenetic alterations of the APC gene may differ according to specific breast cancer phenotypes, remains to be elucidated. Our aim was to explore the role of APC methylation in the inflammatory breast cancer (IBC) phenotype. The status of APC gene promoter hypermethylation was investigated in DNA from normal breast tissues, IBC and non-IBC by both conventional and real-time quantitative methylation-specific PCR (MSP). APC methylation levels were compared with APC mRNA and protein levels. Hypermethylation of the APC gene promoter was present in 71% of IBC samples (n=21) and 43% of non-IBC samples (n=30) by conventional MSP (P=0.047). The APC gene also showed an increased frequency of high methylation levels in IBC (in 74% of cases, n=19) vs non-IBC (in 46% of cases, n=35) using a qMSP assay (P=0.048). We observed ...
Adenomatous polyposis coli (APC), a dominantly inherited disorder, has been mapped to chromosome 5q15-q21 by family linkage studies. Cells from patients with deletions in this region, in one case associated with polyposis in a family, have been used to construct human hamster hybrid cell lines that retain either the normal or deleted chromosome 5. These lines have been used to identify markers from the region of the polyposis gene obtained by cloning the ends of 0.5- to 2-megabase BssHII fragments purified by pulsed-field gel electrophoresis. Three markers are described that map within the deletions and must therefore be close to the APC gene.
What is familial adenomatous polyposis fap? Learn about familial adenomatous polyposis fap symptoms, familial adenomatous polyposis fap causes, diagnosis, and...
Compare & find the top performing anti-Rat (Rattus) Adenomatous Polyposis Coli antibody for Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)).
Regulation and Functions of Localized RNAs. It is becoming increasingly appreciated that, virtually in all polarized mammalian cells, a large number of mRNAs do not exist diffusely in the cytoplasm, but undergo specific subcellular targeting and local control of their translation. Such localized RNAs are important for various processes such as migration, epithelial cell polarity, mitotic spindle assembly and neuronal function. Defects in RNA localization are implicated in diseases such as mental retardation and cancer metastasis. Our lab aims to understand the mechanisms and regulation of RNA localization in mammalian cells, the effect of localized translation on protein function, and the contribution of these processes to disease.. A. The APC-dependent RNA localization pathway. We have identified an RNA localization pathway that targets numerous mRNAs to cellular protrusions (Mili et al, Nature 2008). A central component of this pathway is the tumor suppressor protein adenomatous polyposis coli ...
TY - JOUR. T1 - Some fine-structural fi ndings on the thyroid gland in Apc1638T/1638T mice that express a C-terminus lacking truncated Apc. AU - Yokoyama, Atsushi. AU - Nomura, Ryuji. AU - Kurosumi, Masafumi. AU - Shimomura, Atsushi. AU - Onouchi, Takanori. AU - Iizuka-Kogo, Akiko. AU - Smits, Ron. AU - Fodde, Riccardo. AU - Itoh, Mitsuyasu. AU - Senda, Takao. PY - 2012/6/1. Y1 - 2012/6/1. N2 - Adenomatous polyposis coli (Apc) is a multifunctional protein as well as a tumor suppressor. To determine the functions of the C-terminal domain of Apc, we examined Apc1638T/1638T mice that express a truncated Apc lacking the C-terminal domain. The Apc1638T/1638T mice were tumor free and exhibited growth retardation. We recently reported abnormalities in thyroid morphology and functions of Apc1638T/1638T mice, although the mechanisms underlying these abnormalities are not known. In the present study, we further compared thyroid gland morphology in Apc1638T/1638T and Apc+/+ mice. The diameters of thyroid ...
RATIONALE: Exisulind may be effective in preventing the development and growth of polyps in patients who have familial adenomatous polyposis.. PURPOSE: Randomized phase II/III trial to determine the effectiveness of exisulind in preventing the development and growth of polyps in patients who have familial adenomatous polyposis. ...
Proteins that are associated with the cytoplasmic domain of adhesion molecules such as cadherins and link them to the cytoskeleton. A second role is in regulating transcription factors. α-Catenin (906 aa) is a vinculin-related protein involved in adherens junction-mediated intercellular adhesion; abnormalities are associated with cancer invasion and metastasis. β-Catenin (781 aa) binds E-cadherin and N-cadherin. A second pool of β-catenin is cytoplasmic and coimmunoprecipitates with the adenomatous polyposis coli (APC) tumour suppressor protein, interacts with Tcf and Lef transcription factors, and is an essential member of the Wingless wnt signal transduction pathway. Ubiquitination of β-catenin is greatly reduced in Wnt-expressing cells. See dapper. Mutations in catenin B1 are associated with colorectal cancer, ovarian and prostate carcinomas, hepatoblastoma, hepatocellular carcinoma, pilomatrixoma, and medulloblastoma. γ-Catenin (desmoplakin-3, plakoglobin, 745 aa), is a major component of
The adenomatous polyposis coli (APC) gene is mutated in familial adenomatous polyposis and in many sporadic colorectal tumors. The carboxyl-terminal S/TXV motif of the APC gene product interacts with the PDZ domain of hDLG, the human homolog of the Drosophila lethal (1) discs larige-1 (dlg) tumor su …
Background: The Adenomatous Polyposis Coli (APC) gene is considered as a gatekeeper in colorectal tumorigenesis. 60% of somatic mutations in the APC gene are concentrated..
1578 The Adenomatous Polyposis Coli (APC) gene is a tumor suppressor gene which is associated with both familial and sporadic cancer. Aberrant methylation of the APC promoter 1A which inactives the APC gene occurs in colorectal tumors as well as many other kinds of cancers. We investigated whether the same mechanism occurs in adult T-cell leukemia/lymphoma (ATL) by analyzing 31 DNA samples from 30 ATL patients and 4 ATL cell lines. APC promoter methylation was found in 15 of 31 (48 %) primary samples, and 2 of 4 (50 %) ATL cell lines. Moreover, methylation of the APC gene occured more frequently in acute ATL (12/21) (57 %) than chronic ATL (1/8) (13 %) (P=0.03). APC promoter was not methylated in any of ten peripheral blood samples from normal individuals. APC was expressed in the normal peripheral blood samples, but not in the APC-methylated ATL cell line ST1. Demethylation with 5-Azacytidine treatment restored APC expression in the ST1 cell line. Our data show for the first time that ...
Toll-like receptors (TLRs)/NF-B activation activated by lipopolysaccharide (LPS) was connected with different natural response in cancer of the colon, but the fundamental mechanism was generally unidentified. cell autophagy, which really is a promising therapeutic technique for improving rays therapy [9]. To the very best of our understanding, adenomatous polyposis coli (APC) tumor suppressor pathway stands the prominent as a powered hereditary alteration in colorectal tumorigenesis by aberrant signaling. Almost 20% situations of cancer of the colon are connected with familial clustering, and familial adenomatous polyposis (FAP) is normally a well-defined hereditary susceptibility to colorectal cancers[10C12]. Nevertheless, the systems that is situated between FAP and colorectal cancers is normally indistinct, the tumorigenesis which is the consequence of multiple elements. Included in this, APC is normally an important factor resulting in deposition of -catenin aswell as oncogenes activation, ...
This chapter provides an overview of several examples of molecular biology applications in this rapidly advancing field. Not all present biomarkers can be detected in tissues that are routinely available in pathology laboratories. There is an ongoing effort to adapt techniques that work well with fresh, unfixed tissues to specimens such as formalin-fixed paraffin-embedded needle biopsy material and cytology smear material. Ligase chain reaction and similar techniques are presently used for the detection of microorganisms in tissues by labeling the oligonucleotides with organism-specific sequences. This process can also be used for detection and quantitation of the expression of specific genes in tissues. The study of familial adenomatous polyposis has demonstrated the presence of adenomatous polyposis coli gene mutations in 5q21. The study of molecular pathways of carcinogenesis has highlighted various molecules that are now therapeutic targets. A relatively simple method of examining hundreds of tissue
Familial adenomatous polyposis (FAP) is a condition that can run in families. Untreated FAP can increase the risk of getting bowel cancer.
In patients with familial adenomatous polyposis, six months of twice-daily treatment with 400 mg of celecoxib, a cyclooxygenase-2 inhibitor, leads to a significant reduction in the number of colorectal polyps.
The Food and Drug Administration (FDA) recently approved celecoxib (Celebrex) as an oral adjunct to the standard care (eg, endoscopic surveillance and surgery) of patients with familial adenomatous polyposis (FAP). Celecoxib, the only 1
Smits, R. and Ruiz, P. and Diaz-Cano, S. and Luz, A. and Jagmohan-Changur, S. and Breukel, C. and Birchmeier, C. and Birchmeier, W. and Fodde, R. ...
Campos FG, Habr Gama A, Kiss DR, Silva EV da, Rawet V, Imperiale AR, Perez R, Silva JH da, Sousa AHS, Gama-Rodrigues JJ. Adenocarcinoma after ileoanal anastomosis for familial adenomatous polyposis: review of risk factors and current surveillance apropos of a case. Journal of Gastrointestinal Surgery. 2005 ; 9 695-702 ...
Familial adenomatous polyposis is an inherited condition in which numerous polyps form mainly in the epithelium of the large intestine.
Compare risks and benefits of common medications used for Familial Adenomatous Polyposis. Find the most popular drugs, view ratings, user reviews, and more...
Learn more about Familial Adenomatous Polyposis at Memorial Hospital DefinitionCausesRisk FactorsSymptomsDiagnosisTreatmentPreventionrevision ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
Familial adenomatous polyposis (FAP) is a colonic cancer syndrome which runs in families. It presents as mutliple adenomas in the colon which will become cancer of colon unless the colon is removed surgically.Though the cancer will present in the thirties, we have seen this presenting even at an age of 9 or 10.This runs in families caused by adenomatous polyposis coli (APC) gene, located on chromosome 5q21 ...
TY - JOUR. T1 - RACK1 downregulates levels of the pro-apoptotic protein Fem1b in apoptosis-resistant colon cancer cells. AU - Subauste, M. Cecilia. AU - Ventura-Holman, T.. AU - Du, L.. AU - Subauste, Jose S.. AU - Chan, Shing Leng. AU - Yu, Victor C.. AU - Maher, Joseph F.. PY - 2009/12/1. Y1 - 2009/12/1. N2 - Evasion of apoptosis plays an important role in colon cancer progression. Following loss of the Apc tumor suppressor gene in mice, the gene encoding Fem1b is upregulated early in neoplastic intestinal epithelium. Fem1b is a pro-apoptotic protein that interacts with Fas, TNFR1 and apaf-1, and increased expression of Fem1b induces apoptosis of cancer cells. Fem1b is a homolog of FeM-1, a protein in Caenorhabditis elegans that is negatively regulated by ubiquitination and proteasomal degradation. To study Fem1b regulation in colon cancer progression, we used apoptotis-sensitive SW480 cells, derived from a primary colon cancer, and their isogenic, apoptosis-resistant counterparts sW620 cells, ...
Colorectal cancer is the second leading cause of cancer incidence and cancer death among adults in Europe. It is estimated for the year 2000 alone that 362,620 people were diagnosed with colorectal cancer, and 198,778 patients died owing to this disease (Ferlay et al., 2001). In more than 80% of the sporadic colorectal cancers, both alleles of the Adenomatous Polyposis Coli (APC) gene are inactivated (Kinzler and Vogelstein, 1996). The APC protein forms - together with Axin and GSK3β - a degradation complex for β-catenin. In this complex, GSK3β phosphorylates β-catenin, which in turn is ubiquitinated and thereby targeted for destruction. Wnt signalling inhibits the degradation complex and hence leads to the cytoplasmic accumulation and entry of β-catenin into the nucleus, where it forms a complex with members of the Pangolin(Pan)/TCF/Lef family of DNA-binding proteins, the putative adaptor protein Legless/BCL9 (Lgs) and the transcriptional regulator Pygopus (Pygo) (Behrens et al., 1996; ...
Gastric cancer (GC) is a common malignancy and frequent cause of cancer-related death. Long non-coding RNAs (lncRNAs) have emerged as important regulators and tissue-specific biomarkers of multiple cancers, including GC. Recent evidence has indicated that the novel lncRNA LINC01133 plays an important role in cancer progression and metastasis. However, its function and molecular mechanism in GC remain largely unknown. LINC01133 expression was detected in 200 GC and matched non-cancerous tissues by quantitative reverse transcription PCR. Gain- and loss-of-function experiments were conducted to investigate the biological functions of LINC01133 both in vitro and in vivo. Insights into the underlying mechanisms of competitive endogenous RNAs (ceRNAs) were determined by bioinformatics analysis, dual-luciferase reporter assays, quantitative PCR arrays, TOPFlash/FOPFlash reporter assay, luciferase assay, and rescue experiments. LINC01133 was downregulated in GC tissues and cell lines, and its low expression
Data sets were analysed with the Bio-Rad LaserPix software. For each time point, the total pixel intensity distribution was compared to the pre-bleach image to select the corresponding region. The two images were then compared by eye to confirm that they did correspond to the same focal plane. The coordinates for the bleach ROIs were used to accurately locate the bleach spots on the pre bleach image, and the mean fluorescence intensity for each ROI was calculated. Several equivalent sized ROIs were also placed on unbleached cells to measure any change in fluorescence due to photobleaching or movement.. To track movement of the cells, an acetate sheet was placed over the computer monitor and each ROI was marked on it as well as the shapes of the cells surrounding it. By aligning the sheet with the appropriate cell shapes, the ROI could be appropriately positioned for each time point. This process was used to position each ROI on the appropriate image for each time point.. Once all ROIs had been ...
Acquisition of truncating mutations in the adenomatous polyposis coli (APC) protein underlies the progression of the majority of sporadic and familial colorectal cancers. summary of APC-antibody specificities for APC. (2005) and includes popular antibodies to APC that were not tested in their study. The relevance of reporting such antibody specificities lies in the central importance of APC to malignancy development. MATERIALS AND METHODS Cell lines SW480 cells (ECACC, and gift from MRC laboratories, Leicester, UK) were cultivated in DMEM/10%FBS, HCT116 cells (ECACC) were cultivated in McCoys 5a medium/10%FBS, HEK293 cells (ECACC) were cultivated in MEM/10%FBS. All cells were cultivated at 37C with 5% CO2. Immunoprecipitation and Western blot Cells for immunoprecipitation (IP) and Western blot were collected by scraping, resuspended in IP buffer (50?mM Tris-HCl (pH 7.4), 200?mM KAc, 0.5% Triton-X-100, 1?mM AEBSF, 10?(2005). Furthermore, the C-terminal APC antibodies should give the same ...
Supplementary Materials SUPPLEMENTARY DATA supp_44_2_582__index. Interestingly, manifestation of the cell cycle inhibitor p21 (gene, thereby contributing to the local biosynthesis of estrogen from 19 carbon steroids (30,31). In breast cancer cells, LRH-1 manifestation can be induced by estrogen, via ER, and LRH-1 regulates breasts cancer cell development (26,28). Rules of development involves immediate modulation of ER manifestation (28), excitement of ER recruitment to DNA, probably by advertising co-factor recruitment and remodelling of chromatin to a far more open condition (32), and LRH-1 recruitment to regulatory parts of genes that enhance cell development (33). LRH-1 also promotes breasts tumor cell motility and invasion (34). Within the digestive tract, LRH-1 continues to be implicated in intestinal tumour development. Mice heterozygous for an adenomatous polyposis coli (APC) mutation along with a LRH-1 inactivating mutation created fewer intestinal tumours than mice harbouring the APC ...
Adenomatous polyposis coli (APC) proteins localise in the cytoplasm, at the cell cortex and in the nucleus, where they coordinate the negative regulation of Wnt signalling and direct actin and microtubule organisation and function. The mechanisms that determine APC localisation, and how this localisation contributes to the cellular roles of these proteins, however, remain poorly understood. On page 1589, Brooke McCartney and colleagues address these longstanding questions, first by demonstrating that two domains of Drosophila melanogaster APC2 are required for cell cortex localisation, and second by characterising the subsequent impact of this localisation on both Wnt signalling and actin organisation. The authors show that the localisation of APC2 to the cell cortex is dependent on both the ARM repeats, which undergo self-oligomerisation to form higher-order complexes of APC2, and a new domain that contains the C-terminal 30 amino acid residues. The authors propose that APC2 ARM oligomerisation ...
Apparently, not yet. However, promising results from a study in the New England Journal of Medicine capitalize on our understanding of the genetic basis behind colon cancer. Colon cancer arises when four or five genes become mutated. In more than 90% of cases, it is mutations in the adenomatous polyposis coli (APC) gene that initiate colorectal tumours. The researchers investigated whether it was possible to detect APC mutations in fecal DNA using a new developed method known as digital protein truncation, a technique that could identify mutations in a sensitive and specific manner. Stool samples were collected from 28 patients with nonmetastatic colorectal cancers, 18 patients with adenomas that were at least 1 cm in diameter and 28 control patients. The test managed to identify mutations in 26 of the 46 patients with neoplasia (57%), and in none of the control patients.. Although successful in preventing false positives, which is a common occurrence with the current method of checking the ...
Stabilizes microtubules and may regulate actin fiber dynamics through the activation of Rho family GTPases (PubMed:25753423). May also function in Wnt signaling by promoting the rapid degradation of CTNNB1 (PubMed:10021369, PubMed:11691822, PubMed:9823329). This gene encodes a strongly conserved protein that has an N-terminal coiled-coil domain followed by an armadillo domain, five 20-amino acid repeats, and two SAMP domains. This protein promotes the assembly of a multiprotein complex that recruits and phosphorylates the Wnt effector beta-catenin and targets beta-catenin for ubiquitylation and proteasomal degradation. This protein therefore plays a role in the reduction of cytoplasmic levels of beta-catenin which in turn reduces activation of Wnt target genes that play a pivotal role in the pathogenesis of various human cancers. The protein encoded by this gene is closely related to the adenomatous polyposis coli (APC) tumor-suppressor protein and has similar tumor-suppressor effects. This gene also
Colorectal cancer is a disease of sequential accumulation of mutations in the single layer of epithelial cells lining the rostral-caudal axis of the large intestine (2, 3). Mutations in adenomatous polyposis coli (APC) and β-catenin, which are present in nearly 100% of tumors, lead to loss of normal growth control and the formation of small polyps (2). Further mutations in genes, including KRAS and BRAF, lead to exuberant hyperproliferation and the formation of large premalignant adenomas (2, 3). Terminal mutations in p53, or the TGFβ signaling components SMAD4 or the TGFβ receptor, drive transformation to invasive adenocarcinoma with metastatic potential (2, 3). Although this sequence of mutations is well defined, mechanisms underlying the susceptibility of intestinal epithelial cells to accumulate the mutations driving tumorigenesis remain undefined.. The intestinal epithelium comprises a highly dynamic structure organized as crypt-villus units in small intestine and crypt-surface units in ...
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4G69: Structure of the Human Discs Large 1 PDZ2- Adenomatous Polyposis Coli Cytoskeletal Polarity Complex: Insight into Peptide Engagement and PDZ Clustering.
1jpp: Molecular mechanisms of beta-catenin recognition by adenomatous polyposis coli revealed by the structure of an APC-beta-catenin complex.
Dr Arati Khanna Gupta, VP - R&D with MedGenome Labs, Bengaluru said, Once family members had consented to share their clinical histories and blood samples, we at MedGenome used high throughput Next Generation Sequencing (NGS) methods to look for the FAP causative mutant gene. Our analyses revealed the presence of a never-before-identified mutation in the APC gene in ten of the twenty five family members, six of whom had FAP. Mutations in the APC gene are commonly found in cases of FAP, causing the APC gene product to malfunction. Under normal conditions, APC is a tumour-suppressor gene which prevents uncontrolled growth of cells; a hallmark of cancerous tumours. This is the first-ever genetic study on FAP conducted in India on a large family. It underscores the power of genetic analysis in identifying individuals in the affected family at the risk of developing colorectal cancer, and saving lives through early detection and timely treatment ...
Sigma-Aldrich offers abstracts and full-text articles by [Razvan L Miclea, Marcel Karperien, Cathy Aj Bosch, Geertje van der Horst, Martin A van der Valk, Tatsuya Kobayashi, Henry M Kronenberg, Georges Rawadi, Pinar Akçakaya, Clemens Wgm Löwik, Riccardo Fodde, Jan Maarten Wit, Els C Robanus-Maandag].
New findings are taking researchers and scientists one step closer to a better understanding of autism and mental retardation, the causes of which have long
Certain genetic diseases are known to increase your risk of bowel cancer including Lynch syndrome, Familial Adenomatous Polyposis and MUTYH Associated Polyposis.
Question - Getting blood in the stool. Done with sigmadoscopy. What are the findings?. Ask a Doctor about diagnosis, treatment and medication for Familial adenomatous polyposis, Ask a Pediatrician
"A role for the Adenomatous Polyposis Coli protein in chromosome segregation". Nature Cell Biology. 3 (4): 429-432. doi:10.1038/ ... The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/ ... BubR1 protein levels are shown to decline with age. Furthermore, loss of BubR1 in young organisms is associated with rapid ... Mitotic checkpoint serine/threonine-protein kinase BUB1 beta is an enzyme that in humans is encoded by the BUB1B gene. Also ...
"A role for the Adenomatous Polyposis Coli protein in chromosome segregation". Nature Cell Biology. 3 (4): 429-32. doi:10.1038/ ... Mitotic checkpoint protein BUB3 is a protein that in humans is encoded by the BUB3 gene. Bub3 is a protein involved with the ... cancer adenomatous polyposis osteosarcoma familial breast cancer glioblastoma cervicitis lung cancer carcinoma Coli polyposis ... The complex of proteins which regulate the cell arrest are BUB1, BUB2, BUB3 (this protein), Mad1, Mad2, Mad3 and MPS1. At ...
Through its N-terminal regulator of G-protein signaling (RGS) domain, it recruits the adenomatous polyposis coli (APC) protein ... and in particular by the adenomatous polyposis coli (APC) protein, encoded by the tumour-suppressing APC gene. Therefore, ... its partner the Adenomatous Polyposis Coli (APC) protein contains 11 such motifs in tandem arrangement per protomer, thus ... "Association and regulation of casein kinase 2 activity by adenomatous polyposis coli protein". Proceedings of the National ...
"Association and regulation of casein kinase 2 activity by adenomatous polyposis coli protein". Proc. Natl. Acad. Sci. U.S.A. ... "Characterization of protein interaction among subunits of protein kinase CKII in vivo and in vitro". Mol. Cells. KOREA. 8 (1): ... "Mapping of the interaction domain of the protein kinase CKII beta subunit with target proteins". Mol. Cells. Korea (South). 12 ... a novel pleckstrin homology domain-containing protein that interacts with protein kinase CK2". J. Biol. Chem. UNITED STATES. ...
"Association and regulation of casein kinase 2 activity by adenomatous polyposis coli protein". Proc. Natl. Acad. Sci. U.S.A. 99 ... Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as ... Kim MS, Lee YT, Kim JM, Cha JY, Bae YS (February 1998). "Characterization of protein interaction among subunits of protein ... Allende JE, Allende CC (1995). "Protein kinases. 4. Protein kinase CK2: an enzyme with multiple substrates and a puzzling ...
Rubinfeld B, Tice DA, Polakis P (2001). "Axin-dependent phosphorylation of the adenomatous polyposis coli protein mediated by ... Segment polarity protein dishevelled homolog DVL-1 is a protein that in humans is encoded by the DVL1 gene. DVL1, the human ... Fagotto F, Jho Eh, Zeng L, Kurth T, Joos T, Kaufmann C, Costantini F (1999). "Domains of axin involved in protein-protein ... Kishida S, Yamamoto H, Hino S, Ikeda S, Kishida M, Kikuchi A (1999). "DIX domains of Dvl and axin are necessary for protein ...
Rubinfeld B, Tice DA, Polakis P (2001). "Axin-dependent phosphorylation of the adenomatous polyposis coli protein mediated by ... The encoded protein interacts with adenomatosis polyposis coli, catenin (cadherin-associated protein) beta 1, glycogen synthase ... Adenomatous polyposis coli, CREB-binding protein/(CBP) and might be affected in similar ways by missense mutations of their ... "GSK-3beta-dependent phosphorylation of adenomatous polyposis coli gene product can be modulated by beta-catenin and protein ...
The protein encoded by this gene shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene ... "The adenomatous polyposis coli-binding protein EB1 is associated with cytoplasmic and spindle microtubules". Proc. Natl. Acad. ... a new member of the adenomatous polyposis coli-binding EB1-like gene family, is differentially expressed in activated T cells ... Microtubule-associated protein RP/EB family member 2 is a protein that in humans is encoded by the MAPRE2 gene. ...
The protein encoded by this gene was first identified by its binding to the APC (Adenomatous polyposis coli) protein which is ... "The adenomatous polyposis coli-binding protein EB1 is associated with cytoplasmic and spindle microtubules". Proc. Natl. Acad. ... which is why EB1 is categorized as a microtubule plus end tracking protein(+TIP protein). The protein also associates with ... Microtubule-associated protein RP/EB family member 1 is a protein that in humans is encoded by the MAPRE1 gene. ...
She is known for her work on the role of the adenomatous polyposis coli (APC) protein in colorectal cancer. Näthke grew up in ... Penman, George A.; Leung, Louie; Näthke, Inke S. (2005-10-15). "The adenomatous polyposis coli protein (APC) exists in two ... "The adenomatous polyposis coli tumor suppressor protein localizes to plasma membrane sites involved in active cell migration". ... In her postdoctoral work she established a link between the adenomatous polyposis coli (APC) tumor suppressor and cell movement ...
"Siah-1 mediates a novel beta-catenin degradation pathway linking p53 to the adenomatous polyposis coli protein". Molecular Cell ... The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The ... October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. ... E3 ubiquitin-protein ligase SIAH1 is an enzyme that in humans is encoded by the SIAH1 gene. This gene encodes for a polypeptide ...
It is also dependent on several microtubule-associated proteins such as EB1 and adenomatous polyposis coli (APC). Growth of ... Cortical Dynein, a motor protein moves along the microtubules of the cell and plays a key role in the growth and inhibition of ...
... of the tumor suppressor protein adenomatous polyposis coli (APC). Its structure and its interaction with chromosome maintenance ... XPO1 mediates NES-dependent protein transport. It exports several hundreds of different proteins from the nucleus. XPO1 is ... "Ran-binding protein 3 is a cofactor for Crm1-mediated nuclear protein export". J. Cell Biol. 153 (7): 1391-402. doi:10.1083/jcb ... is a eukaryotic protein that mediates the nuclear export of various proteins and RNAs. XPO1 (CRM1) originally was identified in ...
Zumbrunn J, Kinoshita K, Hyman AA, Näthke IS (Jan 2001). "Binding of the adenomatous polyposis coli protein to microtubules ... Tubulin alpha-4A chain is a protein that in humans is encoded by the TUBA4A gene. Microtubules of the eukaryotic cytoskeleton ... Kirsch J, Langosch D, Prior P, Littauer UZ, Schmitt B, Betz H (Nov 1991). "The 93-kDa glycine receptor-associated protein binds ... Huby RD, Carlile GW, Ley SC (Dec 1995). "Interactions between the protein-tyrosine kinase ZAP-70, the proto-oncoprotein Vav, ...
2004). "The tumor suppressor adenomatous polyposis coli and caudal related homeodomain protein regulate expression of retinol ... 2003). "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and ... transmembrane proteins: a bioinformatics assessment". Genome Res. 13 (10): 2265-70. doi:10.1101/gr.1293003. PMC 403697. PMID ...
... and scaffold proteins adenomatous polyposis coli (APC) and axin targets plakoglobin for degradation.[31-33]. The phosphorylated ... "The tyrosine kinase substrate p120cas binds directly to E-cadherin but not to the adenomatous polyposis coli protein or alpha- ... "The tyrosine kinase substrate p120cas binds directly to E-cadherin but not to the adenomatous polyposis coli protein or alpha- ... Swope D, Cheng L, Gao E, Li J, Radice GL (Mar 2012). "Loss of cadherin-binding proteins β-catenin and plakoglobin in the heart ...
"ARM domain-dependent nuclear import of adenomatous polyposis coli protein is stimulated by the B56 alpha subunit of protein ... "Direct activation of protein phosphatase-2A0 by HIV-1 encoded protein complex NCp7:vpr". FEBS Letters. 401 (2-3): 197-201. doi: ... "The B56alpha regulatory subunit of protein phosphatase 2A is a target for regulation by double-stranded RNA-dependent protein ... Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell ...
"The tyrosine kinase substrate p120cas binds directly to E-cadherin but not to the adenomatous polyposis coli protein or alpha- ... Cadherin-1 or Epithelial cadherin (E-cadherin), (not to be confused with the APC/C activator protein CDH1) is a protein that in ... Complete loss of E-cadherin protein expression in 84% of lobular breast carcinomas. Several proteins such as SNAI1/SNAIL, ... CDH1+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) GeneReviews/NCBI/NIH/UW entry on ...
"The tyrosine kinase substrate p120cas binds directly to E-cadherin but not to the adenomatous polyposis coli protein or alpha- ... "The tyrosine kinase substrate p120cas binds directly to E-cadherin but not to the adenomatous polyposis coli protein or alpha- ... Oneyama C, Nakano H, Sharma SV (March 2002). "UCS15A, a novel small molecule, SH3 domain-mediated protein-protein interaction ... "Entrez Gene: CTNNA1 catenin (cadherin-associated protein), alpha 1, 102kDa". "Protein sequence of human CTNNA1 (Uniprot ID: ...
She showed that checkpoint proteins have to 'sense' that both the adenomatous polyposis coli and EB1 proteins to support normal ... but not clear why aneuploidy can still occur in cells with checkpoint proteins. Draviam demonstrated that checkpoint proteins ... Dravian, Viji Mythily; Raff, Jordan W (2003). "The ch-TOG/XMAP215 protein is essential for spindle pole organization in human ... They study kinetochore proteins through selective mutations and investigations using single molecule microscopy. Specifically, ...
Li Q, Dashwood RH (Oct 2004). "Activator protein 2alpha associates with adenomatous polyposis coli/beta-catenin and Inhibits ... Transcription factor AP-2 alpha (Activating enhancer binding Protein 2 alpha), also known as TFAP2A, is a protein that in ... "The epsins define a family of proteins that interact with components of the clathrin coat and contain a new protein module". ... "Huntingtin interacting protein 1 Is a clathrin coat binding protein required for differentiation of late spermatogenic ...
... has been found to target and inhibit gene expression of the adenomatous polyposis coli (APC) protein, enabling it to ... FOXO1 protein expression is down-regulated in breast tumour tissue samples; miR-27a has been identified as one of three miRNAS ... There is activation of Wnt signalling through nuclear accumulation of this protein, which is in response to inhibition of the ... Suppression of miR-27a results in a FOXO1 protein increase and a consequent cell number decrease. Landgraf, P; Rusu, M; ...
"The tyrosine kinase substrate p120cas binds directly to E-cadherin but not to the adenomatous polyposis coli protein or alpha- ... "The tyrosine kinase substrate p120cas binds directly to E-cadherin but not to the adenomatous polyposis coli protein or alpha- ... p120, and called catenin delta-1 is a protein that in humans is encoded by the CTNND1 gene. This gene encodes a member of the ... The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 4 (2): 141-50. ...
... of the tumor suppressor protein adenomatous polyposis coli (APC). Its structure and its interaction with chromosome maintenance ... Ran (RAs-related Nuclear protein) also known as GTP-binding nuclear protein Ran is a protein that in humans is encoded by the ... Ran is a small G protein that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran ... "Molecular interactions between the importin alpha/beta heterodimer and proteins involved in vertebrate nuclear protein import ...
This later activity is mediated by formins, the adenomatous polyposis coli protein, and EB1, a protein that tracks along the ... MAP-1 proteins consists of a set of three different proteins: A, B and C. The C protein plays an important role in the ... including the motor proteins dynein and kinesin, microtubule-severing proteins like katanin, and other proteins important for ... Plus end tracking proteins are MAP proteins which bind to the tips of growing microtubules and play an important role in ...
This gene encodes (i.e. directs production of) the adenomatous polyposis coli protein (APC protein) but when mutated in FAP ... In the majority of cases, GF tumors are manifestations of the genetic disease, familial adenomatous polyposis (FAP), or its ... APC protein acts indirectly to degrade β-catenin, a protein which when overexpressed induces various cancers. Partially or ... "Familial Adenomatous Polyposis Syndrome: An Update and Review of Extraintestinal Manifestations". Archives of Pathology & ...
"GSK-3beta-dependent phosphorylation of adenomatous polyposis coli gene product can be modulated by beta-catenin and protein ... "Direct activation of protein phosphatase-2A0 by HIV-1 encoded protein complex NCp7:vpr". FEBS Letters. 401 (2-3): 197-201. doi: ... Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell ... Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform is an enzyme that in humans is encoded by the ...
"GSK-3beta-dependent phosphorylation of adenomatous polyposis coli gene product can be modulated by beta-catenin and protein ... The product of this gene belongs to the Protein phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of ... "Direct activation of protein phosphatase-2A0 by HIV-1 encoded protein complex NCp7:vpr". FEBS Letters. 401 (2-3): 197-201. doi: ... Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform is an enzyme that in humans is encoded by the ...
2006). "Adenomatous Polyposis Coli (APC) Is Required for Normal Development of Skin and Thymus". PLOS Genet. 2 (9): e146. doi: ... Kinesin-associated protein 3 (KAP3) is a protein that in humans is encoded by the KIFAP3 gene. It is a non-motor, accessory ... The small G protein GDP dissociation stimulator (smg GDS) is a regulator protein having two activities on a group of small G ... The protein encoded by this gene contains 9 Armadillo repeats and interacts with the smg GDS protein through these repeats. ...
... an anti-coagulant and anti-inflammatory protein Adenomatous polyposis coli, a tumor suppressor protein encoded by the APC gene ... a family of transport proteins APC Superfamily, a superfamily of transport proteins APC tablet, analgesic compound of aspirin, ... a protein from the light-harvesting phycobiliprotein family Allylpalladium chloride dimer, a chemical compound Ammonium ... mutations in which can cause colon cancer Anaphase-promoting complex, a ubiquitin ligase cell cycle protein Antigen-presenting ...
Moreover, the UPS regulates the degradation of tumor suppressor gene products such as adenomatous polyposis coli (APC) in ... To recognize protein as designated substrate, 19S complex has subunits that are capable to recognize proteins with a special ... Accordingly, misfolded proteins and damaged protein need to be continuously removed to recycle amino acids for new synthesis; ... The 19S regulatory particles can recognize ubiquitin-labeled protein as degradation substrate, unfold the protein to linear, ...
Moreover, the UPS regulates the degradation of tumor suppressor gene products such as adenomatous polyposis coli (APC) in ... The eukaryotic proteasome recognizes degradable proteins, including damaged proteins for protein quality control purpose or key ... "Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes". ... This protein is one of the 17 essential subunits that contribute to the complete assembly of the 20S proteasome complex. In ...
The gene associated with colorectal cancer is the adenomatous polyposis coli (APC), which is a classic tumor suppressor gene. ... coordinating the cellular localization of proteins, activating and inactivating proteins, and modulating protein-protein ... The protein modifications can be either a single ubiquitin protein (monoubiquitylation) or a chain of ubiquitin ( ... The monoubiquitination of a protein can have different effects to the polyubiquitination of the same protein. The addition of a ...
APC is a tumour suppressor gene which is associated with the inherited disease adenomatous polyposis coli (APC). It is thought ... Heppner Goss K, Trzepacz C, Tuohy TM, Groden J (June 2002). "Attenuated APC alleles produce functional protein from internal ...
Moreover, the UPS regulates the degradation of tumor suppressor gene products such as adenomatous polyposis coli (APC) in ... "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. Bibcode:2005Natur. ... Lecossier D, Bouchonnet F, Clavel F, Hance AJ (2003). "Hypermutation of HIV-1 DNA in the absence of the Vif protein". Science. ... Such protein accumulation may contribute to the pathogenesis and phenotypic characteristics in neurodegenerative diseases, ...
... such as formation of adenoma via Adenomatous polyposis coli (APC) mutation. Studies on LGR5 in colorectal cancer revealed a ... is a protein that in humans is encoded by the LGR5 gene. It is a member of GPCR class A receptor proteins. R-spondin proteins ... Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) also known as G-protein coupled receptor 49 (GPR49) or G- ... "LGR5 leucine-rich repeat-containing G protein-coupled receptor 5". Entrez Gene. "LGR5 leucine-rich repeat containing G protein- ...
Moreover, the UPS regulates the degradation of tumor suppressor gene products such as adenomatous polyposis coli (APC) in ... Misfolded proteins and damaged protein need to be continuously removed to recycle amino acids for new synthesis; in addition, ... To recognize proteins as designated substrates, the 19S complex has subunits that are capable of recognizing proteins with a ... The 19S regulatory particles can recognize ubiquitin-labeled protein as a substrate for degradation, unfold the protein to a ...
PLAG1 Adenomatous polyposis coli; 175100; APC Adenosine deaminase deficiency, partial; 102700; ADA Adenosine triphosphate, ... HRG Thrombophilia due to protein C deficiency, autosomal dominant; 176860; PROC Thrombophilia due to protein C deficiency, ... GPR56 Polyposis syndrome, hereditary mixed, 2; 610069; BMPR1A Polyposis, juvenile intestinal; 174900; BMPR1A Polyposis, ... OPN1SW Colorectal adenomatous polyposis, autosomal recessive, with pilomatricomas; 132600; MUTYH Colorectal cancer; 114500; ...
Moreover, the UPS regulates the degradation of tumor suppressor gene products such as adenomatous polyposis coli (APC) in ... The eukaryotic proteasome recognized degradable proteins, including damaged proteins for protein quality control purpose or key ... "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957-68. doi:10.1016/j.cell ... "The co-chaperone CHIP regulates protein triage decisions mediated by heat-shock proteins". Nature Cell Biology. 3 (1): 93-6. ...
Muniappan BP, Thilly WG (June 2002). "The DNA polymerase beta replication error spectrum in the adenomatous polyposis coli gene ... An amino acid change may not result in appreciable changes in protein structure depending on whether the amino acid change is ... "Nonsense but not missense mutations can decrease the abundance of nuclear mRNA for the mouse major urinary protein, while both ... and confer transcriptome diversity and enhanced protein function in response to selective pressures. Nonsense mutation Start ...
Moreover, the UPS regulates the degradation of tumor suppressor gene products such as adenomatous polyposis coli (APC) in ... The eukaryotic proteasome recognized degradable proteins, including damaged proteins for protein quality control purpose or key ... This protein has a major role in the immune system as part of an immunoproteasome that is primarily induced upon infection and ... The human protein proteasome subunit beta type-8 is 25 kDa in size and composed of 234 amino acids. The calculated theoretical ...
Moreover, the UPS regulates the degradation of tumor suppressor gene products such as adenomatous polyposis coli (APC) in ... To recognize protein as designated substrate, 19S complex has subunits that are capable to recognize proteins with a special ... Accordingly, misfolded proteins and damaged protein need to be continuously removed to recycle amino acids for new synthesis; ... The 19S regulatory particles can recognize ubiquitin-labeled protein as degradation substrate, unfold the protein to linear, ...
... and EGFR/Ras that impact the growth control and developed a fly model for studying Adenomatous polyposis coli, the colon cancer ... Development (doi: 10.1242/dev.161844). Shukla, J.P., Deshpande, G. and Shashidhara, L.S. (2017). Ataxin-2 binding protein 1 is ... Drosophila melanogaster Homeotic selector gene Adenomatous polyposis coli Ultrabithorax India portal Biology portal 25 of his ... "ChIP for Hox Proteins from Drosophila Imaginal Discs". Hox Genes. Methods in Molecular Biology. Vol. 1196. pp. 241-253. doi: ...
Moreover, the UPS regulates the degradation of tumor suppressor gene products such as adenomatous polyposis coli (APC) in ... Li T, Duan W, Yang H, Lee MK, Bte Mustafa F, Lee BH, Teo TS (Jan 2001). "Identification of two proteins, S14 and UIP1, that ... Lecossier D, Bouchonnet F, Clavel F, Hance AJ (May 2003). "Hypermutation of HIV-1 DNA in the absence of the Vif protein". ... Such protein accumulation may contribute to the pathogenesis and phenotypic characteristics in neurodegenerative diseases, ...
... the majority of which have increased Wnt signaling due to mutations the adenomatous polyposis coli (APC), AXIN2, or rarely the ... Naked cuticle 1 (NKD1) is a human gene that encodes the protein Nkd1, a member of the Naked cuticle (Nkd) family of proteins ... Vertebrate proteins related to Drosophila Naked Cuticle bind Dishevelled and antagonize Wnt signaling. Dev Biol. 2001 Jun 1;234 ... family of proteins (DVL1, DVL2, DVL3). Specific truncating NKD1 mutations identified in DNA mismatch repair deficient colon ...
2009). "Somatic mutations of adenomatous polyposis coli gene and nuclear b-catenin accumulation have prognostic significance in ... Beta-catenin-interacting protein 1 is a protein that is encoded in humans by the CTNNBIP1 gene. The protein encoded by this ... 1 regulates beta-catenin at membrane ruffles and its role in macropinocytosis of N-cadherin and adenomatous polyposis coli". J ... 2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi: ...
... and adenomatous polyposis coli in mammals. DUF383: Domain of unknown function 383 DUF384: Domain of unknown function 383 Every ... "Predicted: brain protein 16-like [Danio rerio]". NCBI Protein. Retrieved 5 February 2013. "Predicted: brain protein 16-like [ ... BRP16 (brain protein 16) is an alias of FAM203A, and BRP16L (brain protein 16-like) is an alias of FAM203B. A gene named BRP16L ... The BRP16L protein in D. rerio has a significant 3' truncation compared to the M. mulatta protein, further supporting the ...
This destruction complex includes the following proteins: Axin, adenomatosis polyposis coli (APC), protein phosphatase 2A (PP2A ... Minde DP, Radli M, Forneris F, Maurice MM, Rüdiger SG (2013). "Large extent of disorder in Adenomatous Polyposis Coli offers a ... "Large extent of disorder in Adenomatous Polyposis Coli offers a strategy to guard Wnt signalling against point mutations". PLOS ... Dsh proteins are present in all organisms and they all share the following highly conserved protein domains: an amino-terminal ...
Moreover, the UPS regulates the degradation of tumor suppressor gene products such as adenomatous polyposis coli (APC) in ... The PSMA5 protein has further been associated with the biosynthesis of conjugated linoleic acid (CLA) in mammary tissue. GRCh38 ... Lecossier D, Bouchonnet F, Clavel F, Hance AJ (May 2003). "Hypermutation of HIV-1 DNA in the absence of the Vif protein". ... Such protein accumulation may contribute to the pathogenesis and phenotypic characteristics in neurodegenerative diseases, ...
... encoding protein Amyloid beta A4 precursor protein-binding family B member 3 APC: adenomatosis polyposis coli ARL15: encoding ... Familial Adenomatous Polyposis is caused by a deletion of the APC tumor suppressor gene on the long (q) arm of chromosome 5. ... encoding protein Proline-rich protein 16 PURA: Purine-rich element-binding protein A PWWP2A: encoding protein PWWP domain ... Protein Red IRX1: Iroquois-class homeodomain protein (human) LARP1: La-related protein 1 LMAN2: Lectin mannose binding 2 LNCR3 ...
The APC gene provides instructions for making the APC protein, which plays a critical role in several cellular processes. Learn ... The adenomatous polyposis coli protein: the Achilles heel of the gut epithelium. Annu Rev Cell Dev Biol. 2004;20:337-66. doi: ... Goss KH, Groden J. Biology of the adenomatous polyposis coli tumor suppressor. J Clin Oncol. 2000 May;18(9):1967-79. doi: ... Senda T, Shimomura A, Iizuka-Kogo A. Adenomatous polyposis coli (Apc) tumor suppressor gene as a multifunctional gene. Anat Sci ...
Adenomatous polyposis coli (APC) also known as deleted in polyposis 2.5 (DP2.5) is a protein that in humans is encoded by the ... The (Adenomatous Polyposis Coli) APC protein normally builds a "destruction complex" with glycogen synthase kinase 3-alpha and ... Zumbrunn J, Kinoshita K, Hyman AA, Näthke IS (January 2001). "Binding of the adenomatous polyposis coli protein to microtubules ... Li Q, Dashwood RH (October 2004). "Activator protein 2alpha associates with adenomatous polyposis coli/beta-catenin and ...
Suppressor_APC; Adenomatous polyposis coli tumour suppressor protein. Alternate T2T-CHM13v2.0. Genomic * NC_060933.1 Alternate ... General protein information Go to the top of the page Help Preferred Names. suppressor APC domain-containing protein 2. Names. ... mRNA and Protein(s) * XM_011519180.4 → XP_011517482.1 suppressor APC domain-containing protein 2 isoform X1 ... mRNA and Protein(s) * NM_178448.4 → NP_848543.2 suppressor APC domain-containing protein 2 ...
DAP-1 was found to associate with hDLG, PSD-95, NMDA-R and adenomatous polyposis coli protein (APC). Furthermore, we found that ... Background: The human homologue of the Drosophila discs large tumour suppressor protein (hDLG) and closely related proteins ... DAP-1, a novel protein that interacts with the guanylate kinase-like domains of hDLG and PSD-95 Genes Cells. 1997 Jun;2(6):415- ... Results: We have identified a protein named DAP-1 that binds to the guanylate kinase-like domains of hDLG and PSD-95. ...
Hanson, C. A. & Miller, J. R. Non-traditional roles for the Adenomatous Polyposis Coli (APC) tumor suppressor protein. Gene 361 ... A role for the Adenomatous Polyposis Coli protein in chromosome segregation. Nature Cell Biol. 3, 429-432 (2001) ... Etienne-Manneville, S. & Hall, A. Cdc42 regulates GSK-3β and adenomatous polyposis coli to control cell polarity. Nature 421, ... Kita, K., Wittmann, T., Nathke, I. S. & Waterman-Storer, C. M. Adenomatous polyposis coli on microtubule plus ends in cell ...
Adenomatous polyposis coli gene mutation and decreased wild-type p53 protein expression in oral submucous fibrosis: a ... Evaluation of plasma fibrinogen degradation products and total serum protein concentration in oral submucous fibrosis. J Clin ...
Adenomatous Polyposis Coli Protein. Orner GA, Dashwood W-M, Blum CA, G Díaz D, Li Q, Al-Fageeh M, Tebbutt N, Heath JK, Ernst M ... Cytoskeletal Proteins. Orner GA, Dashwood W-M, Blum CA, G Díaz D, Li Q, Al-Fageeh M, Tebbutt N, Heath JK, Ernst M, Dashwood RH ...
... and ASTRAL compendium for protein structure and sequence analysis ... adenomatous polyposis coli protein. Species: Homo sapiens [ ... Compound: Beta-catenin-interacting protein 1. Species: Homo sapiens [TaxId:9606]. Gene: CTNNBIP1,ICAT. Database cross- ... SCOPe: Structural Classification of Proteins - extended. Release 2.08 (updated 2022-09-21, stable release September 2021) ...
The Adenomatous Polyposis Coli Protein Is an Essential Regulator of Radial Glial Polarity and Construction of the Cerebral ... and G protein-coupled receptor signals that contribute to neural development.. Expand. ...
2004) Neuronal nicotinic synapse assembly requires the adenomatous polyposis coli tumor suppressor protein. J Neurosci 24:6776- ... 2008) Adenomatous polyposis coli plays a key role, in vivo, in coordinating assembly of the neuronal nicotinic postsynaptic ... 2007) Wnt-7a induces presynaptic colocalization of α7-nicotinic acetylcholine receptors and adenomatous polyposis coli in ... Green fluorescent protein (GFP) fusion proteins for SAP102, SAP97, and PSD-93 (SAP102-GFP, SAP97-GFP, and PSD-93-GFP, ...
... and the role of F-actin in dendritic protein trafficking. ... and the role of F-actin in dendritic protein trafficking. ... Actin is a versatile and ubiquitous cytoskeletal protein that plays a major role in both the establishment and the maintenance ... Actin is a versatile and ubiquitous cytoskeletal protein that plays a major role in both the establishment and the maintenance ... 2010). Adenomatous polyposis coli protein nucleates actin assembly and synergizes with the formin mDia1. J. Cell Biol. 189, ...
These data highlight a dual role for DISC1 in corticogenesis and indicate that phosphorylation of this protein at S710 ... proteins to the centrosome. In support of this model, loss of BBS1 leads to defects in migration, but not proliferation, ... The adenomatous polyposis coli protein is an essential regulator of radial glial polarity and construction of the cerebral ... Figure 1: Phosphorylation of DISC1 at S710 selectively increases binding of DISC1 with BBS proteins, resulting in enhanced BBS1 ...
Dikovskaya, Khoudoli, Newton, Chadha, Klotz, Visvanathan, Lamond, Swedlow, Näthke: "The adenomatous polyposis coli protein ... The encoded protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits ... The protein encoded by this gene inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This ... This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms ...
The cadherin-catenin complex is expressed alternately with the adenomatous polyposis coli protein during rat incisor ... Antibodies to extracellular matrix proteins were also studied to determine the temporal sequence between expression of ... immunoreactive protein for basement membrane proteins, which serve as markers of embryonic induction of nephron development, ... wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary ...
... and include such proteins as beta-catenin, the junctional plaque protein plakoglobin, the adenomatous polyposis coli (APC) ... The adenomatous polyposis coli tumor suppressor protein is also implicated in beta-catenin signaling. ... Crystal structure of the complex between the armadillo repeat domain of adenomatous polyposis coli and the tyrosine-rich domain ... The Structure of a beta-Catenin Binding Repeat from Adenomatous Polyposis Coli (APC) in Complex with beta-Catenin. ...
The adenomatous polyposis coli (APC) gene is a tumor suppressor gene, and mutations resulting in loss of APC protein function ... Adenomatous polyposis coli (APC) plays multiple roles in the intestinal and colorectal epithelia. Med Mol Morphol. 2007;40:68- ... autosomal dominant neoplastic syndrome of Familial Adenomatous Polyposis Coli (FAP). This condition has an annual incidence of ... Multiple colorectal adenomas, classic adenomatous polyposis, and germ-line mutations in MYH. N Engl J Med. 2003;348:791-799. ...
Siah-1 mediates a novel β-catenin degradation pathway linking p53 to the adenomatous polyposis coli protein. J Liu, J Stevens, ... Protein phosphatase 2A and its B56 regulatory subunit inhibit Wnt signaling in Xenopus. X Li, HJ Yost, DM Virshup, JM Seeling ... RNA splicing is interrupted by heat shock and is rescued by heat shock protein synthesis. HJ Yost, S Lindquist ...
"The tyrosine kinase substrate p120cas binds directly to E-cadherin but not to the adenomatous polyposis coli protein or alpha- ... Oneyama C, Nakano H, Sharma SV (March 2002). "UCS15A, a novel small molecule, SH3 domain-mediated protein-protein interaction ... Several proteins such as SNAI1/SNAIL,[54][55] ZFHX1B/SIP1,[56] SNAI2/SLUG,[57][58] TWIST1[59] and DeltaEF1[60] have been found ... Cadherin-1 also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin is a protein that in humans is encoded by ...
Following protein pathways can lead to unexpected places. In this case, three groups approached b-catenin from separate d ... Kinzlers group at Johns Hopkins University independently reported that the tumor suppressor protein adenomatous polyposis coli ... Following protein pathways can lead to unexpected places. In this case, three groups approached b-catenin from separate d. The ... Following protein pathways can lead to unexpected places. In this case, three groups approached b-catenin from separate ...
Bi-allelic Loss of Human APC2, Encoding Adenomatous Polyposis Coli Protein 2, Leads to Lissencephaly, Subcortical Heterotopia, ...
Activator protein 2alpha associates with adenomatous polyposis coli/beta-catenin and Inhibits beta-catenin/T-cell factor ... Activator protein 2alpha associates with adenomatous polyposis coli/beta-catenin and Inhibits beta-catenin/T-cell factor ... Activator protein 2alpha associates with adenomatous polyposis coli/beta-catenin and Inhibits beta-catenin/T-cell factor ... Activator protein 2alpha associates with adenomatous polyposis coli/beta-catenin and Inhibits beta-catenin/T-cell factor ...
APC E1544TER, Adenomatous polyposis coli protein, APC_HUMAN Icon Locations in the PathwayBrowser Expand all ... A protein modification that effectively removes or replaces an L-glutamic acid. ...
APC R499TER, Adenomatous polyposis coli protein, APC_HUMAN Icon Locations in the PathwayBrowser Expand all ...
Alterations of beta-catenin expression, and its binding partners E-cadherin and the adenomatous polyposis coli protein (APC), ... in addition to its binding partners E-cadherin and the adenomatous polyposis coli protein (APC), are frequent events in ... Effects of BEP neuron transplants in the hypothalamus on DMH-induced changes in EMT marker proteins levels in the colon. (A-D) ... Effects of BEP neuron transplants in the hypothalamus on DMH-induced changes in NK cell cytolytic proteins levels in the colon ...
... alterations in intracellular signal transduction proteins (Adenomatous polyposis coli, β-catenin), causing constitutive pathway ... alterations in intracellular signal transduction proteins (Adenomatous polyposis coli, β-catenin), causing constitutive pathway ... alterations in intracellular signal transduction proteins (Adenomatous polyposis coli, β-catenin), causing constitutive pathway ... alterations in intracellular signal transduction proteins (Adenomatous polyposis coli, β-catenin), causing constitutive pathway ...
... and that the protein adenomatous polyposis coli (APC) may play a role in regulating these interactions. The research presented ... This thesis research investigates the regulation of N-cadherin-dependent cell adhesion by bone morphogenetic protein-2 (BMP-2) ... BMP-2 upregulation of N-cadherin expression and resultant chondrogenesis required mitogsn activated protein kinase (MAPK) ... and by day 5 an 8-fold upregulation of N-cadherin protein. ... and that the protein adenomatous polyposis coli (APC) may play ...
... through subtractive genomics approach and host-pathogen protein-protein interactions (HP-PPIs). We also carried out enrichment ... Hence, proteins from IBDsite, a database for inflammatory bowel disease (IBD) research and those involved in colorectal ... These target proteins might be considered for designing potent inhibitors to abrogate F. nucleatum infections. From enrichment ... Subtractive genomics analysis provided a set of target proteins suggested to be indispensable for survival and pathogenicity of ...
... in beta-catenins role as a co-transcriptional factor with the DNA binding proteins TCF (T cell factor) and LEF-1 (lymphoid ... a multi-protein destruction complex consisting of the scaffolding/tumor suppressor proteins Axin and adenomatous polyposis coli ... The F-box protein beta-TrCP associates with phosphorylated beta-catenin and regulates its activity in the cell. Curr. Biol. 9, ... The E3 ligase NLK-associated Ring Finger Protein (NARF) ubiquitinates TCF/LEF-1 in vitro and in vivo, which leads to TCF/LEF-1 ...
  • Familial adenomatous polyposis (FAP) is caused by an inherited, inactivating mutation in the APC gene. (wikipedia.org)
  • More than 800 mutations[citation needed]in the APC gene have been identified in families with classic and attenuated types of familial adenomatous polyposis. (wikipedia.org)
  • The most common mutation in familial adenomatous polyposis is a deletion of five bases in the APC gene. (wikipedia.org)
  • Although APC -related desmoid tumors are commonly associated with a form of colon cancer called familial adenomatous polyposis (described below), APC gene mutations can cause tumors in individuals without this inherited disease. (medlineplus.gov)
  • Mutations in the APC gene are also responsible for a disorder called Turcot syndrome, which is closely related to familial adenomatous polyposis. (medlineplus.gov)
  • Intra-abdominal DTs are often observed in patients with an association of familial adenomatous polyposis (FAP) or Gardner syndrome (see these terms). (rarehematologynews.com)
  • The observed variant frequency is approximately 2 fold of the estimated maximal expected allele frequency for a pathogenic variant in APC causing Familial Adenomatous Polyposis phenotype (7.1e-05), strongly suggesting that the variant is benign. (utah.edu)
  • A has been reported in the literature in individuals affected with Familial Adenomatous Polyposis, colorectal cancer, HBOC, ALL and Neuroblastoma (e.g. (utah.edu)
  • These reports do not provide unequivocal conclusions about association of the variant with Familial Adenomatous Polyposis. (utah.edu)
  • The adenomatous polyposis coli (APC) gene was analyzed for germline mutations in 113 familial adenomatous polyposis suspected families from all over Slovakia. (nel.edu)
  • In the case of multiple intestinal polyps associated with familial adenomatous polyposis (FAP), colon resection remains the only feasible option (see the image below). (medscape.com)
  • Familial adenomatous polyposis, total colectomy specimen. (medscape.com)
  • We further showed that DIM was able to down-regulate survivin and enhance the effects of butyrate in apoptosis induction and prevention of familial adenomatous polyposis in APC min/+ mice. (scialert.net)
  • Adenomatous polyposis coli (APC) is a gene whose mutations are associated with a rare, hereditary form of colorectal cancer known as familial adenomatous. (pasteur.fr)
  • Analysis of candidate modifier loci for the severity of colonic familial adenomatous polyposis, with evidence for the importance of the N-acetyl transferases. (snpedia.com)
  • APC gene mutations in Chinese familial adenomatous polyposis patients. (snpedia.com)
  • Combined with the continuing progress for our novel Ribosome Modulating Agents in RDEB and familial adenomatous polyposis, we believe we are poised to deliver on multiple potential value-creating events over the next twelve months. (eloxxpharma.com)
  • Not all polyposis syndromes are familial. (medscape.com)
  • Familial polyposis syndromes are divided into 2 major groups based on the presence of adenomas or hamartomas. (medscape.com)
  • the familial hamartomatous polyposis syndromes include Peutz-Jeghers syndrome and juvenile polyposis. (medscape.com)
  • Patients with familial adenomatous polyposis harbor colonic biofilms containing tumorigenic bacteria. (vanderbilt.edu)
  • The analysis from the molecular bases of two main syndromes familial adenomatous polyposis (FAP) and hereditary nonpolypsis CRC (HNPCC) provides resulted in the id of two primary pathways where these molecular occasions can result in CRC [5]. (flora2world.com)
  • Hereditary Nonpolyposis Colorectal Cancer (HNPCC) and Familial Adenomatous Polyposis (FAP) are the two most common forms of hereditary predisposition to colorectal cancer. (vallhebron.com)
  • In patients with familial adenomatous polyposis, a genetic disease predisposing to colon cancer, mutations of the APC gene induce the formation of intestinal polyps, but. (newzs.de)
  • Most of these mutations cause the production of an APC protein that is abnormally short and presumably nonfunctional. (wikipedia.org)
  • Most APC gene mutations that cause sporadic desmoid tumors lead to an abnormally short APC protein. (medlineplus.gov)
  • Most of these mutations lead to the production of an abnormally short, nonfunctional version of the APC protein. (medlineplus.gov)
  • The adenomatous polyposis coli ( APC ) gene is a tumor suppressor gene, and mutations resulting in loss of APC protein function are associated with carcinogenesis. (cdc.gov)
  • Wnt-activating mutations can be divided into: i) ligand-independent (LI) alterations in intracellular signal transduction proteins (Adenomatous polyposis coli, β-catenin), causing constitutive pathway activation and ii) ligand-dependent (LD) mutations affecting the synergistic R-Spondin axis (RNF43, RSPO-fusions) acting through amplification of endogenous Wnt signal transmembrane transduction. (elsevier.com)
  • To evaluate the role of the APC/beta-catenin/Tcf pathway, we analyzed mutations in the beta-catenin gene and the accumulation of beta-catenin protein in gastric carcinomas. (springernature.com)
  • Our findings suggest that somatic mutations of the beta-catenin gene are rare in human gastric carcinomas and that accumulation of normal beta-catenin protein in a subset of gastric cancers may be due to other mechanisms of its activation. (springernature.com)
  • In cases with FAP, DTs have been associated with mutations in the tumor suppressor gene APC (5q21-q22) encoding the adenomatous polyposis coli protein . (rarehematologynews.com)
  • In 64 urothelial carcinomas of the urinary bladder, methylationspecific polymerase chain reaction with RAS association domain family 1A (RASSF1A), adenomatous polyposis coli (APC), death-associated protein-kinase (DAPK), runt-related transcription factor 3 (RUNX3), p14, p16 and MGMT was performed and correlated the results with p53 mutations, DNA ploidy, clinicopathologic parameters and recurrences. (jpatholtm.org)
  • Colorectal cancer (CRC) is categorized by alteration of vital pathways such as β-catenin (CTNNB1 ) mutations, WNT signaling activation , tumor protein 53 ( TP53 ) inactivation, BRAF , Adenomatous polyposis coli ( APC ) inactivation, KRAS , dysregulation of epithelial to mesenchymal transition ( EMT ) genes, MYC amplification, etc. (biomedcentral.com)
  • We recently showed that mutations in the adenomatous polyposis coli ( APC ) gene confer resistance to HDAC inhibitor-induced apoptosis in colon cancers. (scialert.net)
  • We used the C10 cell line, which we established and have previously shown to lack adenomatous polyposis coli, E-cadherin, or beta-catenin mutations. (ox.ac.uk)
  • Regardless the importance of GST activity for cellular vitality and health, the GST gene cluster is a hotspot for DNA sequence mutations that leads to the expression of active but functionally different GST variant proteins. (frontiersin.org)
  • ZKN-013 continues to demonstrate dose dependent inducement of functional Collagen VIIA protein (truncated in patients with nonsense mutations patients), in RDEB patient cells. (eloxxpharma.com)
  • The majority of CRC is induced by mutations in adenomatous polyposis coli (transcription followed by metal-induced hydrolysis at 94C. (scienceonstageturkey.com)
  • Unlike HPV-negative HNSCC that is driven by the stepwise accumulation ofmutations in the squamous epithelium, notably mutations in the p53 tumor suppressor gene,HPV-positive HNSCC is caused by two viral oncogenes encoding for early viral proteins, E6 and E7, that bind and inactivate the tumor suppressor genes p53 and pRb leading to malignant transformation of the squamous epithelium. (a-inhibitor.com)
  • The (Adenomatous Polyposis Coli) APC protein normally builds a "destruction complex" with glycogen synthase kinase 3-alpha and or beta (GSK-3α/β) and axin via interactions with the 20 AA and SAMP repeats. (wikipedia.org)
  • APC protein down-regulates the Wnt signaling pathway through its binding to β-catenin and axin ( 8 ). (cdc.gov)
  • In the absence of Wnt binding to the co-receptor complex of Frizzled (Fz) and low-density lipoprotein receptor-related proteins (LRP) 5 and 6, a multi-protein destruction complex consisting of the scaffolding/tumor suppressor proteins Axin and adenomatous polyposis coli (APC), and the serine/threonine kinases casein kinase 1α (CK-1α) and glycogen synthase kinase 3 (GSK3) mediates phosphorylation of β-catenin 2,3,9 . (cytoskeleton.com)
  • PTHR23315: beta-catenin and the subtree to which beta-catenin belongs seems okay #PTHR10845:AN318: Axin #*Subfamily of the RGS (Regulators of G-protein signaling) # PTHR11309 frizzled: 7 distinct subfamilies #* Transmembrane proteins that are receptors for the Wnt signaling proteins. (geneontology.org)
  • Axin Protein" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (uchicago.edu)
  • This graph shows the total number of publications written about "Axin Protein" by people in this website by year, and whether "Axin Protein" was a major or minor topic of these publications. (uchicago.edu)
  • Below are the most recent publications written about "Axin Protein" by people in Profiles. (uchicago.edu)
  • In the absence of a mitotic signal from outside the cell, β-catenin is sequestered in a complex with the adenomatous polyposis coli (APC) gene product, a serine threonine glycogen synthetase kinase (GSK-3β) and an adapter protein axin (or a homologue conductin), enabling phosphorylation and degradation of free β-catenin by the ubiquitin-proteasome system [ 8 ]. (biomedcentral.com)
  • Activation of DsH inhibits another complicated of cytoplasmic proteins including axin, GSK-3 (glycogen synthase kinase-3), as well as the proteins APC (adenomatous polyposis coli). (sprentland.com)
  • In the absence of Wnt signaling, free cytoplasmic β-catenin is rapidly targeted for degradation by a multiprotein complex containing (i) the scaffold proteins axin and adenomatous polyposis coli (APC), and (ii) the serine/threonine kinases casein kinase 1 α (CK1α) and glycogen synthase kinase 3β (GSK3β) [ 10 , 11 ]. (scitechnol.com)
  • DIX domains of Dvl and axin are necessary for protein interactions and their ability to regulate beta-catenin stability. (modencode.org)
  • GSK-3beta-dependent phosphorylation of adenomatous polyposis coli gene product can be modulated by beta-catenin and protein phosphatase 2A complexed with Axin. (modencode.org)
  • Intracellular -catenin amounts are controlled with a complicated made up of axin, casein kinase 1 (CKI)a, and adenomatous polyposis coli (APC). (uitest.info)
  • The second reason is within the destruction complicated in cytoplasm, which is usually assembled from the scaffold protein AXIN, APC, GSK3, and CSNK1A1. (antiviralbiologic.com)
  • In canonical Wnt/β-catenin signaling, the binding of Wnt to Fzd and LRP5/6 results in activation and phosphorylation of the latter, recruitment of Dishvelled and Axin proteins, and inhibition of glycogen synthase kinase (GSK)3β-mediated β-catenin phosphorylation and proteolytic degradation. (scirp.org)
  • It is now clear that Armadillo and beta-catenin bind directly to members of the T-cell factor/lymphoid enhancer factor subfamily of HMG box DNA-binding proteins, forming bipartite transcription factors that regulate Wingless/Wnt responsive genes in both Drosophila and vertebrates. (embl.de)
  • In subtractive genomics approach, the essential, virulence and resistance related proteins were retrieved from RefSeq proteome of F. nucleatum by searching against Database of Essential Genes (DEG), Virulence Factor Database (VFDB) and Antibiotic Resistance Gene-ANNOTation (ARG-ANNOT) tool respectively. (biomedcentral.com)
  • In canonical Wnt-mediated signaling, β-catenin is a key effector and interacts, as a co-transcription factor, with the DNA binding proteins TCF (T cell factor) and LEF-1 (lymphoid enhancer factor 1) to activate the transcription of Wnt/β-catenin target genes including cyclin D1, c-jun, and c-myc 8-11 (Fig. 1). (cytoskeleton.com)
  • a downstream target of GSK3β Glycogen synthase kinase-3 (GSK-3) is a serine/threonine protein kinase encoded by two highly homologous and ubiquitously expressed genes. (web.app)
  • Stabilized β-catenin accumulates in the nucleus where it binds to T cell factor/lymphoid enhancer factor (LEF) and regulates the expression of Snail, Twist, fibronectin, fibroblast-specific protein (FSP)1, matrix metalloproteinase (MMP)-7, plasminogen activator inhibitor (PAI)-1, Ras, and other target genes that promote EMT. (scirp.org)
  • Loss of the APC protein prevents the turnover of p-catenin, and hence p-catenin accumulates in the nucleus and causes the activation of TCF/ LEF or Wnt target genes such as c-Myc and Cyclin D2 (Bienz and Clevers, 2000). (cyberleninka.org)
  • GENEMANIA revealed five genes similar in their expression level with MSH6 and seven genes share the same protein domain with it. (sapub.org)
  • The ras genes (H-, K- and N-) code for highly related proteins of 189 amino acid residues generically known as p21 [ 5 ]. (oeso.org)
  • Genomic techniques such as DNA microarray analysis and proteomic methods such as two-dimensional (2-D) electrophoresis and mass spectrometry are now popular to elucidate the manifestation profiles of genes and proteins. (flora2world.com)
  • 2014). Such hereditary amendments often involve activation of cell development signaling through mutation of aswell as through mutation or epigenetic silencing of vital tumor suppressor genes (TSGs) such as for example p53 and adenomatous polyposis coli (reasonably as dependant on microarray analysis, IFNprotein creation had not been noticeable by ELISA easily, because of low level appearance probably, which was likewise observed also in the FHC handles (Amount 1B). (labrador-kennel.com)
  • Small, monomeric GTP-binding proteins encoded by ras genes (GENES, RAS). (lookformedical.com)
  • The activity of one protein in particular, beta-catenin, is controlled by the APC protein (see: Wnt signaling pathway). (wikipedia.org)
  • Abstract: apc (adenomatous polyposis coli) protein has been thought to be involved in tumor suppression through the wnt/b-catenin signaling pathway. (queenscovecreative.com)
  • Wingless-type MMTV (mouse mammary tumor pathogen) integration site category of the proteins (Wnt) signaling pathway is certainly among those pathways regulating cell proliferation. (sprentland.com)
  • Inhibition of the Wnt signaling pathway by Idax, a novel Dvl-binding protein. (modencode.org)
  • Smoothened (SMO) is one of the constituent proteins of the Hedgehog signaling pathway, and the Hedgehog protein family consists of sonic hedgehog (SHH), Indian hedgehog (IHH), and desert hedgehog (DHH), which bind to the cell surface as a transmembrane protein receptor, Patched (PTCH). (researchsquare.com)
  • Destruction complex function in the Wnt signaling pathway of Drosophila requires multiple interactions between Adenomatous polyposis coli 2 and Armadillo. (cmu.edu)
  • This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. (abnova.com)
  • Actin is a versatile and ubiquitous cytoskeletal protein that plays a major role in both the establishment and the maintenance of neuronal polarity. (frontiersin.org)
  • In this review, we focus on recent developments regarding the role of actin in dendrite morphology, the regulation of actin dynamics by internal and external factors, and the role of F-actin in dendritic protein trafficking. (frontiersin.org)
  • At the plasma membrane β-catenin is an important component of the adherens junctions and links cadherin adhesion proteins to the actin cytoskeleton. (scitechnol.com)
  • Actin cables are assembled at the bud neck by the formin protein Bnr1, and rapidly extend into the mother cell at a rate of 0.5-1 µm/s. (brandeis.edu)
  • In doing so, they identified a novel actin cable 'length sensing' feedback loop, dependent on the myosin-passenger protein Smy1. (brandeis.edu)
  • Adenomatous Polyposis Coli Modulates Actin and Microtubule Cytoskeleton at the Immunological Synapse to Tune CTL Functions. (pasteur.fr)
  • This short protein cannot suppress the cellular overgrowth that leads to the formation of polyps, which can become cancerous. (wikipedia.org)
  • This short protein cannot suppress the cellular overgrowth that leads to the formation of abnormal growths (polyps) in the colon, which can become cancerous. (medlineplus.gov)
  • Studies indicate that the majority of large adenomatous polyps in women will be missed by using flexible sigmoidoscopy alone. (medscape.com)
  • Colonic polyps can occur as part of inherited polyposis syndromes in which their number is greater and the risk for malignant progression is much higher compared to the risk associated with isolated colonic polyps. (medscape.com)
  • FAP is characterized by the presence of hundreds to thousands of adenomatous polyps in the colon and rectum of affected individuals and would lead to colorectal cancer in virtually all the patients if left untreated. (vallhebron.com)
  • 11 Naveau et al found that alcoholism and cirrhosis were two independent risk factors for colorectal adenomatous polyps after successive adjustments for sex, age, smoking, and serum cholesterol levels. (bmj.com)
  • Effects of oral calcium supplementation on intestinal bile acids and cytolytic activity of fecal water in patients with adenomatous polyps of the colon. (jamanetwork.com)
  • Arrestins (abbreviated Arr) are a family of proteins which are important in the regulation of signal transduction G-protein coupled receptors, which at first was discovered, as part of a conserved two step mechanism for regulating the activity of G protein-coupled receptors (GPCR) in rhodopsin visual system by Ursula Kuhn found Scott court, and β-called Global Martin J. Lohse system and kidney and colleagues. (arrestins.com)
  • Ras proteins bind GTP and GDP and possess intrinsic GTPase activity and are involved in signal transduction inducing manifestations of the malignant phenotype. (oeso.org)
  • Colon cancer was induced by injecting 1,2-dimethylhydrazine (DMH) for sixteen weeks in Sprague Dawley rats with BEP neuron transplants or control neuron transplants, and their colonic histopathologies, colon tissue levels of pro-inflammatory cytokines and epithelial-mesenchymal transition (EMT) proteins and splenic levels of cytotoxic proteins were measured. (jcancer.org)
  • Biallelic versions from the APC gene cause colon cancer inside genetic adenomatous polyposis coli (FAP) and also make functionality involving cut down goods inadequate internet domain names associated with beta-catenin wreckage but nevertheless having a minimum size. (bcl-2inhibitors.com)
  • They include LINE-1 insertions interrupting coding exons of the adenomatous polyposis coli (APC) gene, identified first in a case of colon cancer in 1992 and again in an unrelated case this year by Scott Devine's group. (biomedcentral.com)
  • Loss of heterozygosity has been shown in the adenomatous polyposis coli and mutated colon cancer and retinoblastoma locus in Barrett's cancers [ 9 , 10 ]. (oeso.org)
  • We have identified a protein named DAP-1 that binds to the guanylate kinase-like domains of hDLG and PSD-95. (nih.gov)
  • Glycogen synthase kinase-3 is central to multiple intracellular pathways including those activated by Wnt/β-catenin, Sonic Hedgehog, Notch, growth factor/RTK, and G protein-coupled receptor signals that contribute to neural development. (semanticscholar.org)
  • BMP-2 upregulation of N-cadherin expression and resultant chondrogenesis required mitogsn activated protein kinase (MAPK) activity and tyrosine phosphorylation as downstream events. (jefferson.edu)
  • A mitotic checkpoint serine/threonine-protein kinase BUB1 beta that is encoded in the genome of frog. (proconsortium.org)
  • Several derivatives of 5-aminoimidazole-4-carboxamide-1--d-ribofuranoside (AICAR), an AMP-activated protein kinase (AMPK) activator, were identified as transcriptional activators of the DDT gene. (tokushima-u.ac.jp)
  • Proteins normally associated with the apical junctional complexes of epithelial cells, such as Par-3, Par-6 and atypical protein kinase C (aPKC) have a role in polarization in vitro . (biomedcentral.com)
  • Yeast glycogen synthase kinase 3 is involved in protein degradation in cooperation with Bul1, Bul2, and Rsp5. (modencode.org)
  • Regulation of type 1 protein phosphatase/inhibitor-2 complex by glycogen synthase kinase-3beta in intact cells. (modencode.org)
  • It has also been indicated that PM2.5 may trigger asthma, chronic obstructive lung disease (COPD) and lung cancer through the activation of various signaling pathways, including AMP-activated protein kinase (AMPK) catalytic subunit α1 and signal transducer and activator of transcription (STAT)-1 ( 7 ). (spandidos-publications.com)
  • It has also been suggested that vascular endothelial growth factor receptor (VEGF), mitogen-activated protein kinase (MAPK), nuclear factor κB (NF-κB) and interleukin (IL)-8 signaling is involved in PM2.5-induced lung injury ( 8 ). (spandidos-publications.com)
  • Although the function of the CTLH complex remains unclear, here we used yeast two-hybrid screening to isolate Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) as a protein binding to a key component of CTLH complex, Armadillo repeat containing 8 (ARMc8) α. (openbiochemistryjournal.com)
  • Fanning S § , Xu W § , Beaurepaire C, Suhan J, Nantel A, Mitchell A * . Functional control of the Candida albicans cell wall by catalytic protein kinase A subunit Tpk1. (cmu.edu)
  • We have found that down-regulation of APC results in activation of the focal adhesion kinase (FAK) pathway, Src kinase, the STAT3 transcription factor, and epithelial membrane protein 2 (EMP2). (nd.edu)
  • Interaction of protein kinase C zeta with ZIP, a novel protein kinase C-binding protein. (cocites.com)
  • Localization of atypical protein kinase C isoforms into lysosome-targeted endosomes through interaction with p62. (cocites.com)
  • The atypical protein kinase Cs. (cocites.com)
  • The atypical protein kinase C-interacting protein p62 is a scaffold for NF-kappaB activation by nerve growth factor. (cocites.com)
  • In the current study, we demonstrated that upregulation of miR-744 in pancreatic cancer promoted Wnt/β-catenin signaling by directly targeting secreted frizzled-related protein 1 (SFRP1), glycogen synthase kinase 3β (GSK3β), and transducin-like enhancer of split 3 (TLE3), important negative modulators of Wnt/β-catenin signaling. (oncotarget.com)
  • Adenomatous polyposis coli (APC) also known as deleted in polyposis 2.5 (DP2.5) is a protein that in humans is encoded by the APC gene. (wikipedia.org)
  • Precise identification of the genetic cause of this condition has a profound impact on the management of FAP/AFAP family members, and there is therefore an acute need to identify new genetic abnormalities that could be responsible for a significant number of these adenomatous polyposis syndromes in APC/MUTYH mutation negative families. (vallhebron.com)
  • The APC protein is a negative regulator that controls beta-catenin concentrations and interacts with E-cadherin, which are involved in cell adhesion. (wikipedia.org)
  • One protein with which APC associates is beta-catenin. (medlineplus.gov)
  • The shortened protein is unable to interact with the beta-catenin protein, which prevents the breakdown of beta-catenin when it is no longer needed. (medlineplus.gov)
  • The cadherin-catenin complex is expressed alternately with the adenomatous polyposis coli protein during rat incisor amelogenesis. (wikigenes.org)
  • The adenomatous polyposis coli tumor suppressor protein is also implicated in beta-catenin signaling. (embl.de)
  • The separate journeys converged in 1993 when Paul Polakis, then at Onyx Pharmaceuticals Inc. of Richmond, Calif., and Kenneth Kinzler's group at Johns Hopkins University independently reported that the tumor suppressor protein adenomatous polyposis coli (APC) bound b catenin. (the-scientist.com)
  • 2004. Activator protein 2alpha associates with adenomatous polyposis coli/beta-catenin and Inhibits beta-catenin/T-cell factor transcriptional activity in colorectal cancer cells. . (oregonstate.edu)
  • Other laboratories have recently reported that N-cadherin dependent cell adhesion requires intracellular associations with $\alpha $-, $\beta$-, and $\gamma$-catenin, and that the protein adenomatous polyposis coli (APC) may play a role in regulating these interactions. (jefferson.edu)
  • Upon phosphorylation, the E3 ubiquitin ligase β-transducin repeats containing protein (β-TrCP) ubiquitinates phospho-β-catenin on Lys19 and Lys49 13,14 , which leads to proteasomal destruction and low β-catenin levels and activity 15,16 . (cytoskeleton.com)
  • Conversely, in the presence of Wnt binding, the scaffold protein Dishevelled (Dvl) is recruited to the Fz-LRP5/6 co-receptor complex, which activates a sequence of molecular signals that inhibit the destruction complex and result in stabilization of unphosphorylated, cytosolic β-catenin and β-catenin-dependent transcription 2,3,17-19 . (cytoskeleton.com)
  • Moreover, previous binding of APC 3×15 facilitates the association of β-catenin to the TATA box-associated protein. (uab.cat)
  • The protein encoded by APC (Adenomatous polyposis coli), a tumour suppressor gene, plays a key role in the degradation of beta catenin. (gesundheitsindustrie-bw.de)
  • The activated Dsh downregulates the protein complex, so that it can no longer phosphorylate β-catenin, which is then not degraded. (biomedcentral.com)
  • On the lack of Wnt protein, beta catenin is certainly phosphorylated and quickly destructed by ubiquitin-proteaosome.14-16 Some works has indicated that BIO (6-bromoindirubin-3-oxim) can play as GSK-3 inhibitor mimicking the action of Wnt secretive molecules.17 BIO is a derivative of indirubin that is obtained from a trypan purple. (sprentland.com)
  • The data suggest in which interaction among resistance methyltransferases along with the cell's personal ancient methyltransferases can start to play a huge role inside determining weight levels.The adenomatous polyposis coli (APC) proteins are an adverse regulator of the mitogenic transcribing factor beta-catenin by revitalizing it's proteasomal wreckage. (bcl-2inhibitors.com)
  • The 4 20 protein repeats (15R) and also the 20R1 can also be beta-catenin-binding internet sites, but their part in beta-catenin degradation has remained cloudy. (bcl-2inhibitors.com)
  • Emerin interacts with -catenin through a conserved adenomatous polyposis coli (APC)-like domain. (ncl.ac.uk)
  • Immuno-histochemical staining showed the accumulation of β-catenin protein both in cytoplasm as well as in the nuclei of cancer cells when compared with normal tissue. (biomedcentral.com)
  • Here, we aimed to explore the relationship among the Yes-associated protein, β-catenin, and Smoothened signaling pathways to provide a theoretical basis for the clinical diagnosis and Treatment program of invasive breast cancer. (researchsquare.com)
  • Immunohistochemistry was used to detect the expression of Yes-associated protein, Smoothened protein, and β-catenin in breast cancer, adjacent, and normal tissues. (researchsquare.com)
  • the expression of β-catenin and Smoothened protein in breast cancer tissues were all correlated with the human epidermal growth factor receptor expression. (researchsquare.com)
  • The Yes-associated protein, β-catenin, and Smoothened protein can be used as new targets in the treatment of invasive breast cancer or as reference indicators for prognostic judgment. (researchsquare.com)
  • c Assessment of β-catenin/TCF transcriptional activity through a TOPflash reporter assay in adenomatous polyposis coli-mutant DLD1 colorectal cancer cells. (biomedcentral.com)
  • We found that IGF-1 stimulation enhanced tyrosine phosphorylation of two proteins, beta-catenin and insulin-receptor substrate 1, which formed a complex with E-cadherin. (ox.ac.uk)
  • IGF-1 also enhanced the stability of beta-catenin protein. (ox.ac.uk)
  • catenin interacts with this complicated and is after that phosphorylated on three described proteins (Ser33/Ser37/Thr41) by GSK-3 via the ubiquitin-proteasome pathway Rabbit polyclonal to APBB3 [33,35]. (uitest.info)
  • The inner nuclear membrane protein Emerin regulates beta-catenin activity by restricting its accumulation in the nucleus. (core.ac.uk)
  • Protein expression of E-cadherin was increased, whereas that of vimentin, Wnt4, β -catenin, and fibronectin was decreased in Shenning I-treated rats, when compared with the untreated UUO rats, as determined by immunohistochemistry and western blotting (P (scirp.org)
  • beta catenin is also a gene regulatory protein that promotes cellular proliferation. (flashcardmachine.com)
  • APC, (adenomatous polyposis coli), is a tumor suppressor gene whose main function is to down-regulate growth promoting signals, such as beta catenin. (flashcardmachine.com)
  • XX FF beta-catenin links E-cadherin and APC to the cytoskeleton-associated protein alpha-catenin. (genexplain.com)
  • Unphosphorylated DISC1 regulates canonical Wnt signalling via an interaction with GSK3β, whereas specific phosphorylation at serine 710 (S710) triggers the recruitment of Bardet-Biedl syndrome (BBS) proteins to the centrosome. (nature.com)
  • These data highlight a dual role for DISC1 in corticogenesis and indicate that phosphorylation of this protein at S710 activates a key developmental switch. (nature.com)
  • Phosphorylation of DISC1 at S710 selectively increases binding of DISC1 with BBS proteins, resulting in enhanced BBS1 accumulation at the centrosome. (nature.com)
  • Protein post-translational modifications (PTMs) such as phosphorylation, acetylation, ubiquitination, and SUMOylation, to name but a few, have evolved to diversify the functions of a single protein and account for the vast increase in proteome complexity and functional diversity 1 . (cytoskeleton.com)
  • Stabilization occurs through partial inhibition of CK-1α and GSK318, inhibition of β-TrCP20, phosphorylation of five highly conserved PPPSP motifs in LRP6's intracellular domain 21,22 (Fig.1), and subsequent aggregation of these Wnt signaling proteins. (cytoskeleton.com)
  • DIM inhibited survivin mRNA expression and promoted survivin protein degradation through inhibition of p34 cdc2 -cyclin B1-mediated survivin Thr 34 phosphorylation. (scialert.net)
  • Iron is essential for all organisms, and iron-containing proteins catalyze a variety of crucial metabolic processes, such as oxidative phosphorylation and DNA synthesis, as well as cell-cycle progression and growth (Le and Richardson, 2002). (cyberleninka.org)
  • PTHR12607 ADENOMATOUS POLYPOSIS COLI PROTEIN #* Intracellular cytoskeleton, role in structure of adherens junction. (geneontology.org)
  • The protein MAP-1B links GABA(C) receptors to the cytoskeleton at retinal synapses. (cocites.com)
  • Proteins found in the microtubules. (nih.gov)
  • Aided by adenomatous polyposis coli and KIF3A (a kinesin superfamily protein), proteins that travel along microtubules, these apical components accumulate at the tips of growing axons. (biomedcentral.com)
  • Microtubules and their associated motor proteins play a variety of vital roles in orchestrating many processes within higher eukaryotes, such as membrane traffic and cell division. (manchester.ac.uk)
  • Sequestosome 1/p62 is a polyubiquitin chain binding protein involved in ubiquitin proteasome degradation. (cocites.com)
  • The body also uses glutamic acid as a principal digestive enzyme in gastric juice, which controls the degradation of protein to peptones and hydrolyses peptide linkages. (medmelon.gr)
  • The human homologue of the Drosophila discs large tumour suppressor protein (hDLG) and closely related proteins such as postsynaptic density protein 95 kDa (PSD-95) are associated with N-methyl-D-aspartate receptors (NMDA-R) and Shaker-type K+ channels, and are thought to be involved in their clustering. (nih.gov)
  • Results: Gene promoters of SHH (P = 0.016), adenomatous polyposis coli (APC) (P = 0.003), secreted frizzled-related protein 5 (SFRP5) (P = 0.004) and Ras association domain family 1A (RASSF1A) (P = 0.023) showed significantly more methylation in BCC versus normal skin. (maastrichtuniversity.nl)
  • A new paper in Molecular Biology of the Cell from the Rodal lab (in collaboration with the Nicastro lab and the Sokolova Lab at Lomonosov Moscow State University) describes a novel membrane deforming activity for Nervous Wreck (Nwk), an F-BAR protein that regulates trafficking of transmembrane growth signal receptors at the Drosophila neuromuscular junction. (brandeis.edu)
  • Al-Hajj et al demonstrated that Compact disc44+/Compact disc24-/low cells within a breasts tumor, that are cells that express Compact disc44 proteins with detrimental GDC-0449 (Vismodegib) or faint appearance of Compact disc24 proteins, could actually form brand-new tumors in NOD/SCID mice whenever a few hundred of the cells were presented right into a mammary unwanted fat pad [23]. (uitest.info)
  • Interactions of MSH6 with these proteins are critical for its MMR function and any structural alterations that interfere or harm these networks interactions would probably increase susceptibility to tumors formation and progression. (sapub.org)
  • As currently understood, Wnt proteins bind to receptors of the Frizzled and LRP families on the cell surface. (geneontology.org)
  • receptors and their co-receptors are known as as low-density lipoprotein receptor-related proteins 5 and 6 (LRP5/6) and accompanied by activation of Dishevelled family members proteins (DsH) which really is a key element of a membrane-associated Wnt receptor complicated. (sprentland.com)
  • Wnt proteins transmit signals across cell membranes by interacting with Frizzled (Fzd) receptors and their co-receptor low-density lipoprotein receptor-associated protein (LRP)5 or LRP6. (scirp.org)
  • ZIP3, a new splice variant of the PKC-zeta-interacting protein family, binds to GABAC receptors, PKC-zeta, and Kv beta 2. (cocites.com)
  • PMID 17221838 ] Association of adenomatous polyposis coli (APC) gene polymorphisms with autism spectrum disorder (ASD). (snpedia.com)
  • This region of AR-12 manufacturer Escherichia coli 16S rRNA by now consists of several methylated nucleotides such as mirielle(Four)Cm1402 as well as m(Your five)C1407. (bcl-2inhibitors.com)
  • Connections to Escherichia coli are shown below. (vanderbilt.edu)
  • Drug metabolism by Escherichia coli expressing human cytochromes P450. (vanderbilt.edu)
  • In these roles, it binds to cadherins, Tcf-family transcription factors, and the tumor suppressor gene product Adenomatous Polyposis Coli (APC). (embl.de)
  • Cadherin-1 also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin is a protein that in humans is encoded by the CDH1 gene . (wikidoc.org)
  • The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region, and a highly conserved cytoplasmic tail . (wikidoc.org)
  • Complete loss of E-cadherin protein expression in 84% of lobular breast carcinomas. (wikidoc.org)
  • This thesis research investigates the regulation of N-cadherin-dependent cell adhesion by bone morphogenetic protein-2 (BMP-2) during chondrogenesis and the signaling events downstream of BMP-2 and/or N-cadherin required for chondrogenesis. (jefferson.edu)
  • The thesis research presented here demonstrated that within 24 hours of BMP-2 treatment, C3H10T1/2 cells in high density micromass culture exhibit a 4-fold upregulation of N-cadherin mRNA, and by day 5 an 8-fold upregulation of N-cadherin protein. (jefferson.edu)
  • Haas, Andrew Robert, "Chondrogenic induction by bone morphogenetic protein-2: Involvement of N-cadherin-associated functions" (1997). (jefferson.edu)
  • these linkages are almost always indirect, and depend on the intracellular anchorage proteins assembling on the tail of the cadherin. (flashcardmachine.com)
  • About 85% of CRCs occur from occasions that bring about chromosomal instability (CIN) with aneuploidy and early inactivation of adenomatosis polyposis coli (APC). (flora2world.com)
  • The clinical syndrome due to mismatch repair gene deficiency is known as Hereditary Non-Polyposis Colorectal Cancer (HNPCC) and accounts for 2-5% of all colorectal cancer cases. (cdc.gov)
  • Proteins that are related to the frizzled protein family in that they also contain the Wnt-binding site, but SFRP family are secreted and not membrane bound. (geneontology.org)
  • When a mitotic signal is delivered by the Wnt pathway, by association of the Wg/Wnt family of secreted glycoproteins and their membrane receptor frizzled, it leads to activation of the dishevelled (Dsh) protein, which is recruited to the cell membrane. (biomedcentral.com)
  • ZUD (ZU5 and deathdomain-containing protein), also known as UNC5CL (protein unc-5 homolog C-like), is a 518amino acid single-pass type III membrane protein that belongs to the unc-5 family. (sprentland.com)
  • Emerin is a type II inner nuclear membrane (INM) protein of unknown function. (ncl.ac.uk)
  • Sorting and processing of the proteins that span cell membranes requires extensive membrane remodeling , including budding, tubulation, and fission. (brandeis.edu)
  • Until now, it was thought that proteins containing these F-BAR domains induced membrane tubulation by assembling in highly ordered helical coats on lipid bilayers. (brandeis.edu)
  • The work done by the Rodal lab suggests that while basic self-assembly and membrane binding properties are likely conserved between F-BAR proteins, the higher-order organization of Nwk may account for differences in membrane remodeling and its specialized role in the cell. (brandeis.edu)
  • A process in which a protein is transported to, or maintained in, a location within the external part of the cell wall and/or plasma membrane. (princeton.edu)
  • Membrane-anchored mucins may have additional roles concerned with protein interactions at the cell surface. (lookformedical.com)
  • Involved in negative regulation of protein localization to cell cortex and positive regulation of cell population proliferation. (nih.gov)
  • Our research centres on microtubule motor proteins: their regulation, diversity and function, both in healthy cells and how their misfunction may contribute to disease. (manchester.ac.uk)
  • Expression of CARD8 in human atherosclerosis and its regulation of inflammatory proteins in human endothelial cells. (oru.se)
  • The oncogene-derived protein (ONCOGENE PROTEIN P21(RAS)) can play a role in aberrant cellular regulation during neoplastic cell transformation (CELL TRANSFORMATION, NEOPLASTIC). (lookformedical.com)
  • The encoded protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. (antibodies-online.com)
  • Hence, an understanding from the proteins mixed up in diverse stages of apoptosis give chances to discover new goals for treatment strategies [22]. (uitest.info)
  • In addition, our research has shown that the motor protein cytoplasmic dynein is an early target for destruction during programmed cell death, and that disrupting cytoplasmic dynein function in otherwise normal cells can itself trigger apoptosis. (manchester.ac.uk)
  • The bcl-2 proto-oncogene encodes a protein that blocks apoptosis and is involved in the development of follicular lymphoma by a chromosomal translocation. (oeso.org)
  • NMR structure and mutagenesis of the inhibitor-of-apoptosis protein XIAP. (vanderbilt.edu)
  • The ectodomain of this protein mediates bacterial adhesion to mammalian cells, and the cytoplasmic domain is required for internalization. (wikidoc.org)
  • A 235-kDa cytoplasmic protein that is also found in platelets. (umassmed.edu)
  • p62 Is a common component of cytoplasmic inclusions in protein aggregation diseases. (cocites.com)
  • Antibodies are purified by protein a and peptide affinity chromatography. (queenscovecreative.com)
  • Finally, tis sue and cell penetration of antibodies is often less effi cient than of compact chemical substances, as well as target protein requirements to get accessible for the antibody for being efficiently neutralized. (plcsignaling.com)
  • In today's study, we present which the binding properties from the anti-polyQ antibodies MW1 and 3B5H10 support the linear lattice model for the framework of soluble polyQ in the framework of the huntingtin exon 1 fusion proteins. (pik-93.info)
  • This model postulates that Varespladib both extended and regular polyQ tracts in Varespladib the preaggregation condition are random-coil buildings, with extended polyQ repeats filled with more epitopes acknowledged by antibodies or additional binding proteins than normal polyQ tracts [3]. (pik-93.info)
  • Following protein pathways can lead to unexpected places. (the-scientist.com)
  • The apc gene encodes a large 312-kda protein that has been shown to have multiple cellular functions and act through several molecular pathways. (queenscovecreative.com)
  • Our results support recent findings that suggest that INM proteins can influence signalling pathways by restricting access of transcription coactivators to the nucleus. (ncl.ac.uk)
  • P62 and the sequestosome, a novel mechanism for protein metabolism. (cocites.com)
  • Despite the multifunctionality of these proteins, GSTs are best known for their ability to transfer the tripeptide gamma-glutamyl-cysteinyl-glycine, also known as glutathione (GSH) to a wide variety of highly genotoxic and cell-damaging molecules, either directly occurred from the extracellular environment or from the intracellular detoxification metabolism. (frontiersin.org)
  • Bi-allelic Loss of Human APC2, Encoding Adenomatous Polyposis Coli Protein 2, Leads to Lissencephaly, Subcortical Heterotopia, and Global Developmental Delay. (uniklinikum-dresden.de)
  • A second adenomatous polyposis coli (APC)-like gene, APC2/APCL, was recently described and localized to chromosome 19. (sinica.edu.tw)
  • Identification of APC2, a homologue of the adenomatous polyposis coli tumour suppressor. (sinica.edu.tw)
  • Assignment of the murine adenomatous polyposis coli 2 (Apc2) gene to mouse chromosome band 10B5-C2 by in situ hybridisation. (sinica.edu.tw)
  • Eighty-percent of colon cancers stem from a genetic mutation of the protein adenomatous polyposis coli, or APC. (uci.edu)
  • The APC protein helps control how often a cell divides, how it attaches to other cells within a tissue, how the cell polarizes and the morphogenesis of the 3D structures, or whether a cell moves within or away from tissue. (wikipedia.org)
  • This protein also helps ensure that the chromosome number in cells produced through cell division is correct. (wikipedia.org)
  • The APC protein acts as a tumor suppressor, which means that it keeps cells from growing and dividing too fast or in an uncontrolled way. (medlineplus.gov)
  • Furthermore, splenic NK cells cytolytic proteins such as perforin, granzyme B and IFN-γ levels in BEP neurons transplanted rats were higher than those in control rats. (jcancer.org)
  • Identification of protease serine S1 family member 53 as a mitochondrial protein in murine islet beta cells, Islets, Vol.14, No.1, 1-13, 2021. (tokushima-u.ac.jp)
  • FAP is caused by a mutation in the adenomatous polyposis coli (APC) gene coding for APC protein, which acts as a tumor suppressor and keeps cells from growing too fast or in an uncontrolled way. (eloxxpharma.com)
  • EBV-miR-BART12 accelerates migration and invasion in EBV-associated cancer cells by targeting tubulin polymerization-promoting protein 1. (viictr.org)
  • The expression level of Yes-associated protein in the MCF-7 cells was significantly higher than that in the MDA-MB-231 cells. (researchsquare.com)
  • The specification of the ectodermal cells into the epidermal layer known as skin is governed by Wnt signaling stimulation wh-ich helps inhibit the response of ectoderm to fibroblast growth factors (FGFs).25 Without FGFs signaling, the ectodermal cells can express bone morphogenetic proteins (BMPs) and will start to form the epidermal characteristics.25,26. (samploon.com)
  • classical cadherins are probably the most important proteins in adhering like cells, (homophilic adhesion), b/c they only like to bind to other cadherins of the same type. (flashcardmachine.com)
  • We also track the movement of green fluorescent protein-tagged cargoes within living cells using timelapse fluorescence microscopy. (manchester.ac.uk)
  • Now researchers at the Hebrew University of Jerusalem have studied ice-binding "antifreeze proteins" that protect frozen cells from expansion damage when they thaw out. (unitedwithisrael.org)
  • Glutamic acid is one of the 20 amino acids commonly found in proteins - the body's building blocks of cells, enzymes, hormones and immune components. (medmelon.gr)
  • Cancer cells can disrupt a metabolic pathway that breaks down fats and proteins to boost the levels of a byproduct called methylmalonic acid, thereby driving. (newzs.de)
  • Members of the NAP/SET family of proteins interact specifically with B-type cyclins. (modencode.org)
  • Signal integration and diversification through the p62 scaffold protein. (cocites.com)
  • 2009. Activator protein 2alpha suppresses intestinal tumorigenesis in the Apc(min) mouse. . (oregonstate.edu)
  • Functional definition of the mutation cluster region of adenomatous polyposis coli in colorectal tumours. (ous-research.no)
  • Hence, we aimed to predict drug target proteins in F. nucleatum , through subtractive genomics approach and host-pathogen protein-protein interactions (HP-PPIs). (biomedcentral.com)
  • Prediction of HP-PPIs exhibited 186 interactions contributed by 103 host and 76 bacterial proteins. (biomedcentral.com)
  • Project leaders== Varsha Khodiyar, Suzi Lewis ==Justification (Impact and significance)== Wnt proteins form a family of highly conserved secreted signaling molecules that regulate cell-to-cell interactions during embryogenesis. (geneontology.org)
  • Proximity Ligation Analysis of protein-protein interactions between APC and CTNNB1. (abnova.com)
  • Liver fibrosis secondary to bile duct injury: correlation of Smad7 with TGF-beta and extracellular matrix proteins. (shengsci.com)
  • Apc1638t homozygous mice contain a c-terminally deleted apc protein capable of. (queenscovecreative.com)
  • CABS1 is a novel calcium-binding protein specifically expressed in elongate spermatids of mice. (modencode.org)
  • C57BL/6J mice carrying the Min allele of Adenomatous polyposis coli (Apc) develop numerous adenomas along the entire length of the intestine and consequently die at an early age. (isharonline.org)
  • It is not known if this large predicted unstructured region from amino acid 800 to 2843 persists in vivo or would form stabilised complexes - possibly with yet unidentified interacting proteins. (wikipedia.org)
  • This mutation results in the substitution of the amino acid lysine for isoleucine at position 1307 in the APC protein (also written as I1307K or Ile1307Lys). (wikipedia.org)
  • This mutation replaces the amino acid isoleucine with the amino acid lysine at position 1307 in the APC protein (written as Ile1307Lys or I1307K). (medlineplus.gov)
  • A (p.Pro2467Thr) results in a non-conservative amino acid change located in the Adenomatous polyposis coli protein basic domain (IPR009234) of the encoded protein sequence. (utah.edu)
  • The protein encoded by this gene inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). (antibodies-online.com)
  • Rabbit polyclonal antibody against apc protein. (queenscovecreative.com)
  • The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. (arrestins.com)
  • Rabbit polyclonal to ZU5.Proteins containing the death domain (DD) are involved in a wide range of cellular processes,and play an important role in apoptotic and inflammatory processes. (sprentland.com)
  • Rabbit polyclonal antibody raised against a full-length human APC protein. (abnova.com)