A family of large adaptin protein subunits of approximately 130-kDa in size. They have been primarily found as components of ADAPTOR PROTEIN COMPLEX 3.
An adaptor protein complex found primarily on perinuclear compartments.
A clathrin adaptor protein complex primarily involved in clathrin-related transport at the TRANS-GOLGI NETWORK.
An adaptor protein complex primarily involved in the formation of clathrin-related endocytotic vesicles (ENDOSOMES) at the CELL MEMBRANE.
An adaptor protein complex involved in transport of molecules between the TRANS-GOLGI NETWORK and the endosomal-lysosomal system.
The subunits that make up the large, medium and small chains of adaptor proteins.
A family of medium adaptin protein subunits of approximately 45 KDa in size. They have been primarily found as components of ADAPTOR PROTEIN COMPLEX 3 and ADAPTOR PROTEIN COMPLEX 4.
A class of proteins involved in the transport of molecules via TRANSPORT VESICLES. They perform functions such as binding to the cell membrane, capturing cargo molecules and promoting the assembly of CLATHRIN. The majority of adaptor proteins exist as multi-subunit complexes, however monomeric varieties have also been found.
A family of large adaptin protein subunits of approximately 90 KDa in size. They have been primarily found as components of ADAPTOR PROTEIN COMPLEX 1.
A family of large adaptin protein complex subunits of approximately 90-130 kDa in size.
A family of large adaptin protein subunits of approximately 100 kDa in size. They have been primarily found as components of ADAPTOR PROTEIN COMPLEX 2.
Syndrome characterized by the triad of oculocutaneous albinism (ALBINISM, OCULOCUTANEOUS); PLATELET STORAGE POOL DEFICIENCY; and lysosomal accumulation of ceroid lipofuscin.
A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Melanin-containing organelles found in melanocytes and melanophores.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Antibodies produced by a single clone of cells.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.
Indolesulfonic acid used as a dye in renal function testing for the detection of nitrates and chlorates, and in the testing of milk.
Critical analyses of books or other monographic works.
An indole-dione that is obtained by oxidation of indigo blue. It is a MONOAMINE OXIDASE INHIBITOR and high levels have been found in urine of PARKINSONISM patients.
A plant family of the order Lamiales. It is characterized by simple leaves in opposite pairs, cystoliths (enlarged cells containing crystals of calcium carbonate), and bilaterally symmetrical and bisexual flowers that are usually crowded together. The common name for Ruellia of wild petunia is easily confused with PETUNIA.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
A DNA repair enzyme that catalyses the excision of ribose residues at apurinic and apyrimidinic DNA sites that can result from the action of DNA GLYCOSYLASES. The enzyme catalyzes a beta-elimination reaction in which the C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. This enzyme was previously listed under EC 3.1.25.2.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
An enzyme which catalyzes the endonucleolytic cleavage of phosphodiester bonds at purinic or apyrimidinic sites (AP-sites) to produce 5'-Phosphooligonucleotide end products. The enzyme prefers single-stranded DNA (ssDNA) and was formerly classified as EC 3.1.4.30.
Enzymes that catalyze the cleavage of a carbon-oxygen bond by means other than hydrolysis or oxidation. EC 4.2.
A species of orangutan, family HOMINIDAE, found in the forests on the island of Sumatra.
A species of orangutan, family HOMINIDAE, found in the forests on the island of Borneo.
An island in the Malay Archipelago, east of Sumatra, north of Java, and west of Celebes. It is the third largest island in the world. Its name is a Portuguese alteration of BRUNEI, located on it. (From Webster's New Geographical Dictionary, 1988, p163; Room, Brewer's Dictionary of Names, 1992, p73)
A genus of orangutans in the family HOMINIDAE, comprising two species. Among the PRIMATES, the orangutan is second in size only to the gorilla (GORILLA GORILLA).
A republic stretching from the Indian Ocean east to New Guinea, comprising six main islands: Java, Sumatra, Bali, Kalimantan (the Indonesian portion of the island of Borneo), Sulawesi (formerly known as the Celebes) and Irian Jaya (the western part of New Guinea). Its capital is Djakarta. The ethnic groups living there are largely Chinese, Arab, Eurasian, Indian, and Pakistani; 85% of the peoples are of the Islamic faith.
Organic chemistry methodology that mimics the modular nature of various biosynthetic processes. It uses highly reliable and selective reactions designed to "click" i.e., rapidly join small modular units together in high yield, without offensive byproducts. In combination with COMBINATORIAL CHEMISTRY TECHNIQUES, it is used for the synthesis of new compounds and combinatorial libraries.
Any spaces or cavities within a cell. They may function in digestion, storage, secretion, or excretion.
A species of ACTINOMYCES found in the oral cavity of man and hamsters. It has been isolated from actinomycotic lesions in swine, cats, and dogs and has been identified as a causative agent of animal diseases.
A plant genus of the family ASTERACEAE. Members contain pectolinarin (a flavonoid glycoside).
The various physical methods which include wind, insects, animals, tension, and water, by which a plant scatters its seeds away from the parent plant.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
The inter- and intra-relationships between various microorganisms. This can include both positive (like SYMBIOSIS) and negative (like ANTIBIOSIS) interactions. Examples include virus - bacteria and bacteria - bacteria.

The structure and function of the beta 2-adaptin appendage domain. (1/20)

The heterotetrameric AP2 adaptor (alpha, beta 2, mu 2 and sigma 2 subunits) plays a central role in clathrin-mediated endocytosis. We present the protein recruitment function and 1.7 A resolution structure of its beta 2-appendage domain to complement those previously determined for the mu 2 subunit and alpha appendage. Using structure-directed mutagenesis, we demonstrate the ability of the beta 2 appendage alone to bind directly to clathrin and the accessory proteins AP180, epsin and eps15 at the same site. Clathrin polymerization is promoted by binding of clathrin simultaneously to the beta 2-appendage site and to a second site on the adjacent beta 2 hinge. This results in the displacement of the other ligands from the beta 2 appendage. Thus clathrin binding to an AP2-accessory protein complex would cause the controlled release of accessory proteins at sites of vesicle formation.  (+info)

The highly conserved C-terminal dileucine motif in the cytosolic domain of the human immunodeficiency virus type 1 envelope glycoprotein is critical for its association with the AP-1 clathrin adaptor [correction of adapter]. (2/20)

Short amino acid sequences in the cytosolic domains of transmembrane proteins are recognized by specialized adaptor [corrected] proteins which are part of coated vesicles utilized to transport membrane proteins between the trans-Golgi network (TGN) and the plasma membrane (forward and backward). Previously, we and others reported that the membrane-proximal tyrosine residues Y712 (human immunodeficiency virus [HIV]) and Y721 (simian immunodeficiency virus [SIV]) in the envelope glycoprotein (Env) of the primate lentiviruses are crucial for the association of Env with clathrin-associated adaptor [corrected] complex AP-2. The same tyrosine-based endocytosis motifs in the cytosolic domains (EnvCD) of transmembrane gp41 of HIV type 1 (HIV-1) and SIV, respectively, were also shown to modulate the interaction with TGN- and endosome-based clathrin-associated complex AP-1. Our findings suggested that EnvCD binding to AP-1, unlike the association of EnvCD with AP-2, is dependent largely on residues other than Y712 and Y721. Here, we tested if motifs downstream of Y712 affect HIV-1 EnvCD-AP-1 binding and Env trafficking. Mutational analysis revealed that the C-terminal leucine-based motif in Env was crucial for the recruitment of AP-1 in vitro and in Env-expressing cells. In addition to affecting Env-AP-1 association, mutations at the C terminus of Env also altered the subcellular localization of Env, suggesting that proper post-Golgi routing of Env depends on its recruitment of AP-1. Finally, the C-terminal dileucine was shown to assist the membrane-proximal Y712 motif in restricting the cell surface expression of Env.  (+info)

PACS-1 binding to adaptors is required for acidic cluster motif-mediated protein traffic. (3/20)

PACS-1 is a cytosolic protein involved in controlling the correct subcellular localization of integral membrane proteins that contain acidic cluster sorting motifs, such as furin and human immunodeficiency virus type 1 (HIV-1) NEF: We have now investigated the interaction of PACS-1 with heterotetrameric adaptor complexes. PACS-1 associates with both AP-1 and AP-3, but not AP-2, and forms a ternary complex between furin and AP-1. A short sequence within PACS-1 that is essential for binding to AP-1 has been identified. Mutation of this motif yielded a dominant-negative PACS-1 molecule that can still bind to acidic cluster motifs on cargo proteins but not to adaptor complexes. Expression of dominant-negative PACS-1 causes a mislocalization of both furin and mannose 6-phosphate receptor from the trans-Golgi network, but has no effect on the localization of proteins that do not contain acidic cluster sorting motifs. Furthermore, expression of dominant-negative PACS-1 inhibits the ability of HIV-1 Nef to downregulate MHC-I. These studies demonstrate the requirement for PACS-1 interactions with adaptor proteins in multiple processes, including secretory granule biogenesis and HIV-1 pathogenesis.  (+info)

The beta-appendages of the four adaptor-protein (AP) complexes: structure and binding properties, and identification of sorting nexin 9 as an accessory protein to AP-2. (4/20)

Adaptor protein (AP) complexes are essential components for the formation of coated vesicles and the recognition of cargo proteins for intracellular transport. Each AP complex exposes two appendage domains with that function to bind regulatory accessory proteins in the cytosol. Secondary structure predictions, sequence alignments and CD spectroscopy were used to relate the beta-appendages of all human AP complexes to the previously published crystal structure of AP-2. The results suggested that the beta-appendages of AP-1, AP-2 and AP-3 have similar structures, consisting of two subdomains, whereas that of AP-4 lacks the inner subdomain. Pull-down and overlay assays showed partial overlap in the binding specificities of the beta-appendages of AP-1 and AP-2, whereas the corresponding domain of AP-3 displayed a unique binding pattern. That AP-4 may have a truncated, non-functional domain was indicated by its apparent inability to bind any proteins from cytosol. Of several novel beta-appendage-binding proteins detected, one that had affinity exclusively for AP-2 was identified as sorting nexin 9 (SNX9). SNX9, which contains a phox and an Src homology 3 domain, was found in large complexes and was at least partially associated with AP-2 in the cytosol. SNX9 may function to assist AP-2 in its role at the plasma membrane.  (+info)

The delta subunit of AP-3 is required for efficient transport of VSV-G from the trans-Golgi network to the cell surface. (5/20)

Vesicular stomatitis virus glycoprotein (VSV-G) is a transmembrane protein that functions as the surface coat of enveloped viral particles. We report the surprising result that VSV-G uses the tyrosine-based di-acidic motif (-YTDIE-) found in its cytoplasmic tail to recruit adaptor protein complex 3 for export from the trans-Golgi network. The same sorting code is used to recruit coat complex II to direct efficient transport from the endoplasmic reticulum to the Golgi apparatus. These results demonstrate that a single sorting sequence can interact with sequential coat machineries to direct transport through the secretory pathway. We propose that use of this compact sorting domain reflects a need for both efficient endoplasmic reticulum export and concentration of VSV-G into specialized post-trans-Golgi network secretory-lysosome type transport containers to facilitate formation of viral coats at the cell surface.  (+info)

Subunit H of the V-ATPase binds to the medium chain of adaptor protein complex 2 and connects Nef to the endocytic machinery. (6/20)

Nef is an accessory protein of human and simian immunodeficiency viruses (HIV and SIV) that is required for efficient viral infectivity and pathogenicity. It decreases the expression of CD4 on the surface of infected cells. V1H is the regulatory subunit H of the vacuolar membrane ATPase (V-ATPase). Previously, the interaction between Nef and V1H has been found to facilitate the internalization of CD4, suggesting that V1H could connect Nef to the endocytic machinery. In this study, we demonstrate that V1H binds to the C-terminal flexible loop in Nef from HIV-1 and to the medium chain (mu2) of the adaptor protein complex 2 (AP-2) in vitro and in vivo. The interaction sites of V1H and mu2 were mapped to a central region in V1H from positions 133 to 363, which contains 4 armadillo repeats, and to the N-terminal adaptin-binding domain in mu2 from positions 1 to 145. Fusing Nef to V1H reproduced the appropriate trafficking of Nef. This chimera internalized CD4 even in the absence of the C-terminal flexible loop in Nef. Finally, blocking the expression of V1H decreased the enhancement of virion infectivity by Nef. Thus, V1H can function as an adaptor for interactions between Nef and AP-2.  (+info)

Phosphoinositide 3-kinase regulates beta2-adrenergic receptor endocytosis by AP-2 recruitment to the receptor/beta-arrestin complex. (7/20)

Internalization of beta-adrenergic receptors (betaARs) occurs by the sequential binding of beta-arrestin, the clathrin adaptor AP-2, and clathrin. D-3 phosphoinositides, generated by the action of phosphoinositide 3-kinase (PI3K) may regulate the endocytic process; however, the precise molecular mechanism is unknown. Here we demonstrate that betaARKinase1 directly interacts with the PIK domain of PI3K to form a cytosolic complex. Overexpression of the PIK domain displaces endogenous PI3K from betaARK1 and prevents betaARK1-mediated translocation of PI3K to activated beta2ARs. Furthermore, disruption of the betaARK1/PI3K interaction inhibits agonist-stimulated AP-2 adaptor protein recruitment to the beta2AR and receptor endocytosis without affecting the internalization of other clathrin dependent processes such as internalization of the transferrin receptor. In contrast, AP-2 recruitment is enhanced in the presence of D-3 phospholipids, and receptor internalization is blocked in presence of the specific phosphatidylinositol-3,4,5-trisphosphate lipid phosphatase PTEN. These findings provide a molecular mechanism for the agonist-dependent recruitment of PI3K to betaARs, and support a role for the localized generation of D-3 phosphoinositides in regulating the recruitment of the receptor/cargo to clathrin-coated pits.  (+info)

AP-3 directs the intracellular trafficking of HIV-1 Gag and plays a key role in particle assembly. (8/20)

Gag proteins direct the process of retroviral particle assembly and form the major protein constituents of the viral core. The matrix region of the HIV-1 Gag polyprotein plays a critical role in the transport of Gag to the plasma membrane assembly site. Recent evidence indicates that Gag trafficking to late endosomal compartments, including multivesicular bodies, occurs prior to viral particle budding from the plasma membrane. Here we demonstrate that the matrix region of HIV-1 Gag interacts directly with the delta subunit of the AP-3 complex, and that this interaction plays an important functional role in particle assembly. Disruption of this interaction eliminated Gag trafficking to multivesicular bodies and diminished HIV particle formation. These studies illuminate an early step in retroviral particle assembly and provide evidence that the trafficking of Gag to late endosomes is part of a productive particle assembly pathway.  (+info)

We have conducted a hybrid dysgenic screen of the X chromosome for mutations affecting female fertility, with particular attention to those causing abnormal egg and eggshell morphology. In a screen of 4017 dysgenic strains, 398 mutants derived from 168 different germ lines were isolated and assigned to eight classes according to their diverse phenotypes. One interesting class consists of mutants that block oogenesis at specific stages. Our analysis has focused on mutations affecting eggshell formation, including mutants that lay morphologically abnormal sterile eggs as well as those that lay no eggs but exhibit blocks in the late stages of oogenesis. A subset of 48 mutants was assorted into 30 allelic groups by inter se complementation and genetically localized by interval mapping. Two multiallele complementation groups, de1 (7 alleles) and ne1 (8 alleles), were identified as well as five two-allele complementation groups. A search for alleles among mutants generated in other female sterile ...
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Nickel atom in PDB 1qkc: Escherichia Coli Ferric Hydroxamate Uptake Receptor (Fhua) in Complex Delta Two-Albomycin
A membrane electrode assembly for use with a direct organic fuel cell containing a formic acid fuel includes a solid polymer electrolyte having first and second surfaces, an anode on the first surface and a cathode on the second surface and electrically linked to the anode. The solid polymer electrolyte has a thickness t: t ≥ n f D f C f K f j f c where Cf is the formic acid fuel concentration over the anode, Df is the effective diffusivity of the fuel in the solid polymer electrolyte, Kf is the equilibrium constant for partition coefficient for the fuel into the solid polymer electrolyte membrane, ℑ is Faradays constant nf is the number of electrons released when 1 molecule of the fuel is oxidized, and jf c is an empirically determined crossover rate of fuel above which the fuel cell does not operate.
Discovery of activating mutation of the EGFR gene in 2004 opened the era of personalized therapy in thoracic oncology. These tumors are highly dependent on the EGFR pathway and inhibition of this pathway results in dramatic induction of apoptosis in vitro, even though cancer cells may have various genetic alterations (oncogene addiction). These observations were soon translated into clinical trials. Recent 4 phase III clinical trials for patients with EGFR mutation (NEJ 002, WJTOG3405, OPTIMAL, EURTAC) reproducibly showed significantly longer progression free survival for those treated with EGFR-tyrosine kinase inhibitors (TKI) than those treated with platinum doublet chemotherapy. Although there was no difference in overall survival probably due to high crossover rate, it was noteworthy that 40% of patients in EGFR-TKI arm were able to go without platinum doublet and yet had similar survival outcome in WJTOG3405 In 2007, it was found that ∼5% of adenocarcinoma of the lung harbors EML4-ALK ...
A bicycle frame is optimized for the lightest weight by using genetic algorithms in this study. Stresses of five rods in the bicycle frame less than the material yielding strength with consideration of the factor of safety are the constraints. A two-dimensional model of the frame is created. Equilibrium equations are derived and loads acting on rods are determined. A known function is used to verify feasibility of the program generated. Effects of the mutation rate, the crossover rate and the number of generation on the mean and the standard deviation of the fitness value are studied. The optimal solutions with the outer diameters and the inner diameters of the front frame rods to be 0.040 m and 0.038 m, respectively, the outer diameters and the inner diameters of the rear frame rods to be 0.024 m and 0.021m, respectively, and the weight of the bicycle frame to be 0.896 kg are recommended for the bicycle frame.. Copyright © 2007 by ASME ...
TY - JOUR. T1 - Real time measurements of methanol crossover in a DMFC. AU - Han, Jiahua. AU - Liu, Hongtan. PY - 2007/1/10. Y1 - 2007/1/10. N2 - Methanol crossover is a serious problem in a direct methanol fuel cell (DMFC), which causes significant voltage loss and waste of fuel. Due to methanol crossover, most DMFCs must operate on a fuel with a very low methanol concentration; yet very low methanol concentration also causes a poor cell performance. Thus, it is very important to find the optimal operating conditions of methanol concentration and other operating parameters. In this research, methanol crossover rate in a DMFC is determined by measuring the carbon dioxide concentration at the cathode exit in real time. By measuring methanol crossover and cell performances at different inlet methanol concentrations and various operating conditions three types of characteristics are identified in the relationships between methanol crossover and cell current density. Further analysis of these ...
The use of ambipolar redox active materials comprised of electron rich and electron poor groups (donor-acceptor strategy) is proposed to generate molecules with tuneable redox potentials. This allows their implementation on both the positive as negative half-cell of the battery, avoiding capacity loss by crossover. An iterative design strategy will be used to build a library of compounds that will be screened for reversible redox chemistry and stability. New membranes separating the half cells will be developed and matched with redox materials that passed the first screening, testing for compatibility and crossover rates. Finally, all components will be combined and studied in prototype flow batteries. Results from these studies will be used as input for computational modelling in order to predict a new generation of redox compounds and improved membrane design. This ultimately leads to a detailed understanding on their structure-property relations and development of materials that can be used ...
Central Epidural Steroid Injections (ESI) and Selective Nerve Root Blocks (SNRB) are often used for the non-surgical treatment of lumbar disc herniations and lumbar radiculitis (radiating pain). Numerous authors have reported on their value in treating patients with radicular pain with the possibility of delaying or even obviating the need for surgery in well-selected patients.. There are two well-performed clinical studies in the peer-reviewed medical literature that specifically examined the crossover rates to surgery for patients who received either ESI or SNRB. In a prospective study, Buttermann et al. found a crossover rate to surgery for patients with symptomatic disc herniations treated with ESI of 54% (27/50) . In a separate prospective study, Riew et al. followed patients after selective nerve root blocks and found that similarly 53% (29/55) of their patients had avoided surgery after a selective nerve root block during their initial follow-up of 13-28 months. In a later study that ...
AP-3 complex subunit beta-1 is a protein that in humans is encoded by the AP3B1 gene. This gene encodes a protein that may play a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. The encoded protein is part of the heterotetrameric AP-3 protein complex which interacts with the scaffolding protein clathrin. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 2. AP3B1 has been shown to interact with AP3S2. GRCh38: Ensembl release 89: ENSG00000132842 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000021686 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. DellAngelica EC, Ooi CE, Bonifacino JS (Jun 1997). Beta3A-adaptin, a subunit of the adaptor-like complex AP-3. The Journal of Biological Chemistry. 272 (24): 15078-84. doi:10.1074/jbc.272.24.15078. PMID 9182526. Simpson F, Peden AA, Christopoulou L, Robinson MS (May 1997). Characterization of the adaptor-related protein complex, AP-3. The ...
In order to regulate methanol concentration, since the methanol crossover rate is roughly proportional to the methanol concentration at the anode, it is necessary to accurately sense the concentration of methanol in solution. Various types of sensors may be used to measure the concentration of methanol in an aqueous solution. Capacitance devices, which measure the change in the dielectric constant of the methanol solution, can be used as methanol sensors. However, the difference in dielectric constants for a methanol-water system is small leading to less accurate values. A sensing device, in wide range of methanol concentrations, up to about, 10 mol/L, would be very helpful for DMFC optimized operation. A passive mode design, of about 1/10th of a cm area, using a single parameter function, will be designed. The design and simulation would involve optimization of various parameters like the area of the working electrodes and counter electrodes, separation distance and overlap length between ...
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Arsenic atom in PDB 1r4x: Crystal Structure Analys of the Gamma-Copi Appendage Domain
Health related message boards offering discussions of numerous health topics including allergies, cancer, diabetes, heart disease, exercise, attention deficit disorder, diet, and nutrition.
Objectives Our aim was to compare the effectiveness between right radial approach (RRA) and left radial approach (LRA) by means of a randomized study in a large unselected patient population undergoing diagnostic coronary angiography. Methods Totally, 1,000 patients were randomized to undergo to RRA (n = 500) or LRA (n = 500). Procedure success was defined as coronary angiography completed with the initial radial artery approach without changing to another route. Performance of the procedure: Total procedural duration, the number of catheters and guidewires used were recorded. Safety of the procedure: The parameters collected for radiation exposure were dose area product (DAP) and fluoroscopy time. Results The percentage of success was not different between the two aproaches (LRA, 94.0%; RRA,93.8%; P = 0.96). The crossover rate to femoral was low, accounting for 38 cases (3.8%), without differences between RRA and LRA (20 and 18 cases, respectively, P > 0.05). An almost triple incidence of ...
In the fruitfly Drosophila melanogaster, the four male-specific lethal (msl) genes are required to achieve dosage compensation of the male X chromosome. The MSL proteins are thought to interact with cis-acting sites that confer dosage compensation to nearby genes, as they are detected at hundreds of discrete sites along the length of the polytene X chromosome in males but not in females. The histone H4 acetylated isoform, H4Ac16, colocalizes with the MSL proteins at a majority of sites on the D. melanogaster X chromosome. Using polytene chromosome immunostaining of other species from the genus Drosophila, we found that X chromosome association of MSL proteins and H4Ac16 is conserved despite differences in the sex chromosome karyotype between species. Our results support a model in which cis-acting regulatory sites for dosage compensation evolve on a neo-X chromosome arm in response to the degeneration of its former homologue.. ...
The structure of the appendage domain of β2 adaptin is similar to that of α‐adaptin in that it has two subdomains with similar topology, but with a difference of ∼46° in the orientation between the two subdomains. Like the α‐appendage, the β2‐appendage domain binds AP180, epsin and eps15, and therefore is likely to be involved in recruiting proteins involved in controlling CCV formation to sites of coated pit formation in vivo. The two proteins possess a partly conserved binding site of high hydrophobic potential on the C‐terminal platform domain, suggesting that they bind similar motifs i.e. DΦF/W. The binding site residues are also conserved in β1‐adaptin from the adaptor AP1, so this homologue would be predicted to bind the same ligands as β2. Indeed, a GSTβ1 appendage construct does bind to eps15, and weakly to clathrin and epsin (data not shown). Although the α and β2 appendages share some of the same ligands, they bind to them with different relative affinities: the ...
The AP-complex family (Boehm and Bonifacino, 2001; Nakatsu and Ohno, 2003; Owen et al., 2004; Robinson, 2004) has six members in mammals. AP-1A, AP-2, AP-3A and AP-4 are ubiquitously expressed. The other two members, AP-5 and AP-6, are cell-type-specific isoforms of AP-1A and AP-3A: the epithelium-specific AP-1B and the neuron-restricted AP-3B.. The AP complexes consist of four subunits: one small (σ1-σ4), one medium (μ1-μ4) and two large (α, γ, δ or ϵ; and β1-β4) subunits. These assemble to form a structure in which two appendage domains are connected by flexible hinge regions to the core (Owen et al., 2004; Owen and Luzio, 2000; Robinson, 2004). The large subunits are divided into three domains: the N-terminal domain, which makes up the core with the μ and σ subunits; the hinge domain, and the C-terminal appendage. One of the large subunits (α, γ, δ or ϵ) is implicated in binding to the target membrane (Collins et al., 2002; Nakatsu and Ohno, 2003; Owen et al., 2004; Traub, ...
AP-3 complex subunit delta-1 is a protein that in humans is encoded by the AP3D1 gene. AP3D1 is a subunit of the AP3 adaptor- ... "Entrez Gene: AP3D1 adaptor-related protein complex 3, delta 1 subunit". CS1 maint: discouraged parameter (link) Martinez-Arca S ... "Interactions of HIV-1 nef with the mu subunits of adaptor protein complexes 1, 2, and 3: role of the dileucine-based sorting ... Simpson F, Peden AA, Christopoulou L, Robinson MS (May 1997). "Characterization of the adaptor-related protein complex, AP-3". ...
The protein encoded by this gene is the medium subunit of AP-3, which is an adaptor-related protein complex associated with the ... AP-3 is a heterotetrameric protein complex composed of two large subunits (delta and beta3), a medium subunit (mu3), and a ... "Entrez Gene: AP3M1 adaptor-related protein complex 3, mu 1 subunit". Human AP3M1 genome location and AP3M1 gene details page in ... 2000). "Interactions of HIV-1 nef with the mu subunits of adaptor protein complexes 1, 2, and 3: role of the dileucine-based ...
... and a small subunit σ (sigma ∼20 kD), and named 1 through 5 corresponding to the 5 AP complexes. Components of COPI (cop one) a ... In AP-1 it is named γ (gamma), AP-2 has α (alpha), AP-3 has δ (delta), AP-4 has ε (epsilon) and AP-5 has ζ (zeta). The two ... Most of the adaptor proteins are heterotetramers. In the AP complexes, there are two large proteins (∼100 kD) and two smaller ... Vesicular transport adaptor proteins are proteins involved in forming complexes that function in the trafficking of molecules ...
Hanzal-Bayer M, Renault L, Roversi P, Wittinghofer A, Hillig RC (May 2002). "The complex of Arl2-GTP and PDE delta: from ... "Interaction of the Grb7 adapter protein with Rnd1, a new member of the Rho family". FEBS Letters. 467 (1): 91-6. doi:10.1016/ ... Nancy V, Callebaut I, El Marjou A, de Gunzburg J (Apr 2002). "The delta subunit of retinal rod cGMP phosphodiesterase regulates ... Hanzal-Bayer M, Renault L, Roversi P, Wittinghofer A, Hillig RC (May 2002). "The complex of Arl2-GTP and PDE delta: from ...
"The delta subunit of rod specific cyclic GMP phosphodiesterase, PDE delta, interacts with the Arf-like protein Arl3 in a GTP ... Hanzal-Bayer M, Renault L, Roversi P, Wittinghofer A, Hillig RC (2002). "The complex of Arl2-GTP and PDE delta: from structure ... Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (1996). "A "double adaptor" method for improved shotgun library ... ADP-ribosylation factor-like protein 3 is a protein that in humans is encoded by the ARL3 gene. ADP-ribosylation factor-like 3 ...
... the plasma membrane the TCR receptor chains α and β associate with six additional adaptor proteins to form an octameric complex ... The intersection of these specific regions (V and J for the alpha or gamma chain; V, D, and J for the beta or delta chain) ... in the transmembrane domain of each subunit form polar interactions allowing a correct and stable assembly of the complex. The ... LAT associates with another scaffolding protein Slp-76 via the Grap2 adaptor protein, which provides additional binding sites. ...
... adaptor protein complex beta subunits MeSH D12.776.543.990.150.500.300 - adaptor protein complex delta subunits MeSH D12.776. ... adaptor protein complex gamma subunits MeSH D12.776.543.990.150.500.500 - adaptor protein complex mu subunits MeSH D12.776. ... adaptor protein complex subunits MeSH D12.776.543.990.150.500.100 - adaptor protein complex alpha subunits MeSH D12.776.543.990 ... adaptor protein complex 1 MeSH D12.776.543.990.150.200 - adaptor protein complex 2 MeSH D12.776.543.990.150.300 - adaptor ...
... consists of seven subunits which compose the heteroheptameric protein complex. The primary function of adaptors is the ... Color scheme: membrane - gray; Arf1 - pink; gamma-COP - light green; beta-COP, dark green; zeta-COP - yellow; delta-COP - ... COPI is a coatomer, a protein complex[1] that coats vesicles transporting proteins from the cis end of the Golgi complex back ... or adaptor proteins associate with ARFs is unclear, however, in order to form a mature transport carrier coat protein, adaptor ...
2001). "The Transmembrane Adaptor Protein Trim Regulates T Cell Receptor (Tcr) Expression and Tcr-Mediated Signaling via an ... San José E, Sahuquillo AG, Bragado R, Alarcón B (1998). "Assembly of the TCR/CD3 complex: CD3 epsilon/delta and CD3 epsilon/ ... "The implications of subunit interactions for the structure of the T cell receptor-CD3 complex". Eur. J. Immunol. 20 (2): 299- ... T-cell receptor alpha locus is a protein that in humans is encoded by the TRA gene, also known as TCRA or [email protected] It contributes ...
The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two ... Eiraku M, Hirata Y, Takeshima H, Hirano T, Kengaku M (Jul 2002). "Delta/notch-like epidermal growth factor (EGF)-related ... "Entrez Gene: AP1G1 adaptor-related protein complex 1, gamma 1 subunit". Fölsch H, Ohno H, Bonifacino JS, Mellman I (Oct 1999 ... "Similar subunit interactions contribute to assembly of clathrin adaptor complexes and COPI complex: analysis using yeast three- ...
Bellovino D, Morimoto T, Tosetti F, Gaetani S (Jan 1996). "Retinol binding protein and transthyretin are secreted as a complex ... Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (Apr 1996). "A "double adaptor" method for improved shotgun library ... van Leeuwen JE, Kearse KP (Apr 1996). "Calnexin associates exclusively with individual CD3 delta and T cell antigen receptor ( ... Trombetta ES, Simons JF, Helenius A (Nov 1996). "Endoplasmic reticulum glucosidase II is composed of a catalytic subunit, ...
The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are ... The Notch pathway, through transcriptional activation of Delta ligand (see DLL3). MET mediates a complex program known as ... Furge KA, Zhang YW, Vande Woude GF (November 2000). "Met receptor tyrosine kinase: enhanced signaling through adapter proteins ... c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is ...
... mitochondrial processing alpha subunit PRUNE2: protein prune homolog 2 RABGAP1: RAB GTPase activating protein 1 REXO4: RNA ... Transmembrane protein 268 TOR2A encoding protein Torsin-2A TSC1: tuberous sclerosis complex 1 TTC39B: tetratricopeptide repeat ... delta-, dehydratase ALS4: amyotrophic lateral sclerosis 4 ANGPTL2: angiopoietin-related protein 2 ASS: argininosuccinate ... signaling threshold regulating transmembrane adapter 1 SLC25A25-AS1: encoding protein SLC25A25 antisense RNA 1 SNORD24: ...
Translation initiation factor eIF-2B subunit beta is a protein that in humans is encoded by the EIF2B2 gene. Eukaryotic ... "Characterization of the mammalian initiation factor eIF2B complex as a GDP dissociation stimulator protein". J. Biol. Chem. 276 ... Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (1996). "A "double adaptor" method for improved shotgun library ... It consists of alpha (EIF2B1; MIM 606686), beta (EIF2B2), gamma (EIF2B3; MIM 606273), delta (EIF2B4; MIM 606687), and epsilon ( ...
This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in ... WHSC2 encodes the NELF-A subunit of the NELF complex. WHSC2 has been shown to interact with RDBP. GRCh38: Ensembl release 89: ... Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (Apr 1996). "A "double adaptor" method for improved shotgun library ... "Stimulation of RNA polymerase II elongation by hepatitis delta antigen". Science. 293 (5527): 124-7. doi:10.1126/science. ...
... either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also ... 2001). "Newly synthesized human delta opioid receptors retained in the endoplasmic reticulum are retrotranslocated to the ... Protein transport protein Sec61 subunit alpha isoform 1 is a protein that in humans is encoded by the SEC61A1 gene. The protein ... 2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi: ...
"The SH2-containing adapter protein GRB10 interacts with BCR-ABL". Oncogene. 17 (8): 941-8. doi:10.1038/sj.onc.1202024. PMID ... "p210BCR/ABL induces formation of complexes containing focal adhesion proteins and the protooncogene product p120c-Cbl". Exp. ... Rommel C, Camps M, Ji H (2007). "PI3K delta and PI3K gamma: partners in crime in inflammation in rheumatoid arthritis and ... Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform is an enzyme that in humans is encoded by the ...
Ionizable residues in the transmembrane domain of each subunit form a polar network of interactions that hold the complex ... to bind to the ITAM and activated ZAP-70 phosphorylates tyrosines on the adaptor protein LAT, which then attracts PLC-γ. Other ... Likewise, generation of the TCR gamma chain involves VJ recombination, whereas generation of the TCR delta chain occurs by VDJ ... The TCR complex[edit]. The TCR receptor complex is an octomeric complex of variable TCR receptor α and β chains with three ...
... encoding protein 5-azacytidine-induced protein 2 BRK1: SCAR/WAVE actin nucleating complex subunit BRPF1: bromodomain and PHD ... alpha 2/delta subunit 3 CCR5: chemokine (C-C motif) receptor 5 CGGBP1: CGG triplet repeat binding protein 1 CMTM7: CKLF like ... Tumor protein p63 TRAT1: T-cell receptor-associated transmembrane adapter 1 USH3A: Usher syndrome 3A ZBED2: encoding protein ... also known as Glutamine-rich protein 1, RBM6: RNA-binding protein 6 RPP14: Ribonuclease P protein subunit p14 SCN5A: sodium ...
The coatomer subunit delta (delta-COP) is a cytosolic protein complex that binds to motifs and associates with vesicles ... Molecular determinants regulating selective binding of autophagy adapters and receptors to ATG8 proteins. Nat Commun 10, 2055 ( ... Role of Mammalian Vacuolar Protein-sorting Proteins in Endocytic Trafficking of a Non-ubiquitinated G Protein-coupled Receptor ... There is no evidence of post-translational modifications of the TMEM267 protein found in tissues. According to protein sequence ...
AZI2: encoding protein 5-azacytidine-induced protein 2. *BRK1: SCAR/WAVE actin nucleating complex subunit ... CACNA2D3: calcium channel, voltage-dependent, alpha 2/delta subunit 3. *CCR5: chemokine (C-C motif) receptor 5 ... TRAT1: T-cell receptor-associated transmembrane adapter 1. *USH3A: Usher syndrome 3A ... C3orf14-Chromosome 3 open reading frame 14: predicted DNA binding protein.. *C3orf23: encoding protein Uncharacterized protein ...
Leitges M, Gimborn K, Elis W, Kalesnikoff J, Hughes MR, Krystal G, Huber M (June 2002). "Protein kinase C-delta is a negative ... Not only catalytic but also adaptor activities of this protein are involved in this process. Its movement from the cytosol to ... Poe JC, Fujimoto M, Jansen PJ, Miller AS, Tedder TF (June 2000). "CD22 forms a quaternary complex with SHIP, Grb2, and Shc. A ... Zhang S, Broxmeyer HE (January 1999). "p85 subunit of PI3 kinase does not bind to human Flt3 receptor, but associates with SHP2 ...
These changes facilitate the recruitment of specific adapter proteins such as the insulin receptor substrate proteins (IRS) in ... "Differential effects of tumor necrosis factor-alpha on protein kinase C isoforms alpha and delta mediate inhibition of insulin ... Downstream post-translational events of either isoform result in the formation of a proteolytically cleaved α and β subunit, ... Strictly speaking the relationship between IR and ligand shows complex allosteric properties. This was indicated with the use ...
E1A adenovirus protein, and S-HDAg (hepatitis delta virus small delta antigen).[19] p300/CBP have also been observed to ... They usually function within a multisubunit complex in which the other subunits are necessary for them to modify histone ... transcriptional adaptor), TFIID (transcription factor II D), TFTC (TBP-free TAF-containing complex), and NuA3/NuA4 (nucleosomal ... Some of the proteins that associate with HATs in these complexes function by targeting the HAT complex to nucleosomes at ...
The calcium channel consists of a complex of alpha-1, alpha-2/delta and beta subunits in a 1:1:1 ratio. The S3-S4 linkers of ... Calcium channel, voltage-dependent, L type, alpha 1C subunit (also known as Cav1.2) is a protein that in humans is encoded by ... Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (Apr 1996). "A "double adaptor" method for improved shotgun library ... Cav1.2 is a subunit of L-type voltage-dependent calcium channel. This gene encodes an alpha-1 subunit of a voltage-dependent ...
... and that the SH2 domain of p85 specifically recognized phosphotyrosines on growth factor receptors or adaptor proteins via the ... Specifically, they discovered that the catalytic subunit p110 dimerizes with the regulatory subunit p85, ... control mTOR signaling by acting as a GTPase-activating protein complex toward Rheb". Curr. Biol. 13 (15): 1259-68. doi:10.1016 ... The first drug targeting the PI-3-kinase pathway as a treatment for cancer - Idelalisib (PI3K Delta inhibitor) - was approved ...
Bacteria and fungi may form complex biofilms, protecting from immune cells and proteins; biofilms are present in the chronic ... In parallel, when toll-like receptors in the endocytic compartments recognize a virus the activation of the adaptor protein ... where the transglutaminase polymerizes 210 kDa subunits of a plasma-clotting protein. On the other hand, in the horseshoe crab ... Main article: gamma/delta T cells. Like other 'unconventional' T cell subsets bearing invariant T cell receptors (TCRs), such ...
"Expression and characterization of the p85 subunit of the phosphatidylinositol 3-kinase complex and a related p85 beta protein ... a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in ... "Dual regulation of neuronal morphogenesis by a delta-catenin-cortactin complex and Rho". J. Cell Biol. 162 (1): 99-111. doi: ... The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. ...
Each molecule of the dynein motor is a complex protein assembly composed of many smaller polypeptide subunits. Cytoplasmic and ... another multisubunit protein that is essential for mitosis, and a cargo adaptor.[10] The tri-complex, which includes dynein, ... The dynactin complex is composed of more than 20 subunits,[17] of which p150(Glued) is the largest.[18] There is no definitive ... mediates the attachment of several cargo adaptors to the dynein motor.[11] The other tail subunits may also help facilitate ...
Gi alpha subunits (also termed Giα subunits) inhibit adenylyl cyclase b) G beta-gamma complex of subunits (Gβγ) have many ... G-protein coupled receptor signaling pathway. • adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway. • G- ... clathrin and clathrin adaptor AP2, of the receptor internalization machinery. These pathways render DP2 unable to mobilize ... G protein-coupled receptors (GPCRs) such as DP2 are integral membrane proteins that, when bound by their cognate ligands (or, ...
regulation of protein ADP-ribosylation. • positive regulation of protein complex assembly. • positive regulation of protein ... IFNγ suppresses osteoclast formation by rapidly degrading the RANK adaptor protein TRAF6 in the RANK-RANKL signaling pathway, ... including subunits of the immunoproteasome (MECL1, LMP2, LMP7), as well as TAP and ERAAP in addition possibly to the direct ... positive regulation of protein serine/threonine kinase activity. • positive regulation of protein deacetylation. • positive ...
14-3-3 protein zeta/delta (14-3-3ζ) is a protein that in humans is encoded by the YWHAZ gene on chromosome 8. The protein ... For instance, 14-3-3ζ controls cellular senescence by complexing with BIS to chaperone protein folding of STAT3 and activate ... "14-3-3 proteins associate with A20 in an isoform-specific manner and function both as chaperone and adapter molecules". The ... "Human signaling protein 14-3-3zeta interacts with platelet glycoprotein Ib subunits Ibalpha and Ibbeta". Blood. 91 (4): 1295- ...
... the pyrin domain of the adaptor protein ASC has recently been shown to function as a prion-like domain, through a self- ... Caspase-1 then assembles into its active form consisting of two heterodimers with a p20 and p10 subunit each. Once active, it ... ASC helps recruit caspase-1 to associate with NLRs in the inflammasome complex via its CARD domain. ASC also has duties ... "The NLRP3 inflammasome modulates sleep and NREM sleep delta power induced by spontaneous wakefulness, sleep deprivation and ...
... and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon ... The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, ... White boxes represent UTRs (untranslated regions). Orange: protein coding regions. The black lines connecting boxes represent ... Previous Names: "adaptor-related protein complex 3, delta 1 subunit" HgncId : HGNC:568 Omim: 607246 ...
Protein Coding), Adaptor Related Protein Complex 3 Subunit Delta 1, including: function, proteins, disorders, pathways, ... AP3D1 (Adaptor Related Protein Complex 3 Subunit Delta 1) is a Protein Coding gene. Diseases associated with AP3D1 include ... AP3D1 Gene (Protein Coding) Adaptor Related Protein Complex 3 Subunit Delta 1. ... Protein Symbol:. O14617-AP3D1_HUMAN. Recommended name:. AP-3 complex subunit delta-1. Protein Accession:. O14617. Secondary ...
AP-3 complex subunit delta-1 is a protein that in humans is encoded by the AP3D1 gene. AP3D1 is a subunit of the AP3 adaptor- ... "Entrez Gene: AP3D1 adaptor-related protein complex 3, delta 1 subunit". CS1 maint: discouraged parameter (link) Martinez-Arca S ... "Interactions of HIV-1 nef with the mu subunits of adaptor protein complexes 1, 2, and 3: role of the dileucine-based sorting ... Simpson F, Peden AA, Christopoulou L, Robinson MS (May 1997). "Characterization of the adaptor-related protein complex, AP-3". ...
Adaptor related protein complex 3 delta 1 subunit. Protein classi. Protein class(es) of the gene product according to selected ... The cell lines in the Human Protein Atlas have been analyzed by RNA-seq to estimate the transcript abundance of each protein- ... In the Human Protein Atlas a NX value of 1.0 is defined as a threshhold for expression of the corresponding protein.. Read more ... Protein evidencei. Protein evidence scores are generated from several independent sources and are classified as evidence at i) ...
AP-3 is a heterotetrameric protein complex composed of two large subunits (delta and beta3), a medium subunit (mu3), and a ... AP-3 complex subunit mu-1. Names. AP-3 adapter complex mu3A subunit. adapter-related protein complex 3 mu-1 subunit. adaptor ... AP3M1 adaptor related protein complex 3 subunit mu 1 [Homo sapiens] AP3M1 adaptor related protein complex 3 subunit mu 1 [Homo ... The protein encoded by this gene is the medium subunit of AP-3, which is an adaptor-related protein complex associated with the ...
... delta 1 subunit; Adaptor-related protein complex 3 subunit delta-1; adaptor-related protein complex 3, delta 1 subunit; AP-3 ... adapter-related protein complex 3 subunit delta-1; adaptin, delta; adaptor related protein complex 3 subunit delta 1; adaptor ... complex delta subunit, partial CDS; AP-3 complex subunit delta; AP-3 complex subunit delta-1; ap3; delta adaptin; Delta-adaptin ... AP3D1 is a subunit of the AP3 adaptor-like complex, which is not associated with clathrin. The AP3D1 subunit is implicated in ...
Endosomal Sorting Complex Required for Transport; MCT, multi-subunit tethering complex; AP, Adaptor Protein. IDs of genes ... Delta; E, Epsilon; Z, Zeta; T20, Tip20; D1, Dsl1; S39, Sec39; T, Trs; T17, Tca17; C, COG; S, Sec; E, Exo; ESCRT, ... Cytoskeletal proteins include proteins associated with actomyosin motor complex and IMC. All extracellular and cytoskeletal ... Legends at the top of each column denote subunit components of complexes. For each organism, filled sectors of the pie ...
Buy the Paperback Book HIV Interactions with Host Cell Proteins by Paul Spearman at Indigo.ca, Canadas largest bookstore. + ... The authors have recently described an interaction between Gag and the delta subunit of the AP-3 adaptor protein complex (1). ... Title:HIV Interactions with Host Cell ProteinsFormat:PaperbackDimensions:214 pagesPublished:March 1, 2012Publisher:Springer ... HIV Interactions with Host Cell Proteins. EditorPaul Spearman. Paperback , March 1, 2012. ...
AP17,AP17-DELTA,CLAPS2. *Gene Description:. *adaptor-related protein complex 2, sigma 1 subunit ... clathrin assembly protein 2 small chain,clathrin-associated/assembly/adaptor protein, small 2 (17kD) ... One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane ... This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this ...
... the delta subunit of AP-3, for further evaluation. AP-3 is an "adaptor protein complex," a group of four proteins known to sort ... Through a series of experiments, the group demonstrated that the AP-3 delta subunit interacts with Gag to direct it to the ... Spearman s team has focused on a protein called "Gag," the major HIV structural protein. In recent years, Spearman said, it has ... In some cell types, Gag and the HIV viral envelope protein form particles in the multivesicular body; in other cell types, Gag ...
... which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, ... The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of ... which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. ... dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors ( ...
Protein Coding), Calcium Voltage-Gated Channel Auxiliary Subunit Gamma 3, including: function, proteins, disorders, pathways, ... Interacts with AP4M1 and GRIA1; associates GRIA1 with the adaptor protein complex 4 (AP-4) to target GRIA1 to the ... The L-type calcium channel is composed of five subunits: alpha-1, alpha-2/delta, beta and gamma. Acts as an auxiliary subunit ... Protein Symbol:. O60359-CCG3_HUMAN. Recommended name:. Voltage-dependent calcium channel gamma-3 subunit. Protein Accession:. ...
What is Adapter-related protein complex 3 subunit beta-1? Meaning of Adapter-related protein complex 3 subunit beta-1 medical ... What does Adapter-related protein complex 3 subunit beta-1 mean? ... Looking for online definition of Adapter-related protein ... complex 3 subunit beta-1 in the Medical Dictionary? Adapter-related protein complex 3 subunit beta-1 explanation free. ... a protein complex composed of two large subunits (delta and beta), a medium subunit (mu), and a small subunit (sigma). AP3 ...
AP3D1 fusion protein Ag9390 Full name: adaptor-related protein complex 3, delta 1 subunit ... ADTD, AP 3 complex subunit delta, AP 3 complex subunit delta 1, AP3D1, Delta adaptin, hBLVR ... Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and ... AP3D1 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin ( ...
What is clathrin adaptor complex AP3 mu-3A subunit? Meaning of clathrin adaptor complex AP3 mu-3A subunit medical term. What ... clathrin adaptor complex AP3 mu-3A subunit explanation free. ... does clathrin adaptor complex AP3 mu-3A subunit mean? ... Looking for online definition of clathrin adaptor complex AP3 mu-3A subunit in the Medical Dictionary? ... an adaptor-related protein complex composed of two large subunits (delta and beta), a medium subunit (mu), and a small subunit ...
... delta 1 subunit (Ap3d1), F-box protein 38 (Fbxo38), and MON2 homolog (Mon2) were used that remain unaltered after neuronal ... ratio to the housekeeping gene β-actin except for the facial nerve injury experiment for which adaptor-related protein complex ... Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 2001; ... Clinical and biochemical spectrum of D-bifunctional protein deficiency. Ann Neurol. 2006;59(1):92-104.CrossRefGoogle Scholar ...
... and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon ... The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, ... Subunit Structure. AP-3 associates with the BLOC-1 complex (By similarity). Adaptor protein complex 3 (AP-3) is a ... Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi ...
Evaluation of the delta subunit of bovine adaptor protein complex 3 as a receptorfor bovine leukaemia virus. ... to a gene family of the delta subunit of the adaptor protein (AP)complex 3, AP-3. Therefore, bovine cDNAs (boAP3delta) that are ... Click on the protein counts, or double click on taxonomic names to display all proteins containing BLVR domain in the selected ... Complex between Vamp7 longin domain and fragment of delta-adaptin from AP3. ...
... an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more ... In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into ... Subunit structurei. Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit ... Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi ...
... and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi ... The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the ... In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into ... AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related ...
... adapter-related protein complex 2 sigma subunit; clathrin-associated/assembly/adaptor protein, small 2 (17kD); AP17; AP17-DELTA ... adaptor-related protein complex 2, sigma 1 subunit; CLAPS2; AP-2 complex subunit sigma; sigma2-adaptin; HA2 17 kDa subunit; ... clathrin assembly protein 2 small chain; adaptor protein complex AP-2 subunit sigma; plasma membrane adaptor AP-2 17 kDa ... Products > Cancer Proteins > Signal Transduction Proteins > Protein Trafficking > Golgi Proteins Golgi Proteins MAPK3 PLIN2 ...
Mouse Homolog of the Bovine Leukemia Virus Receptor Is Closely Related to the delta Subunit of Adaptor-Related Protein Complex ... The predicted 679-amino acid sequence encoded in these cDNAs is characteristic of an integral membrane protein in that multiple ... We have isolated human complementary DNAs (cDNAs) encoding a protein which, when expressed in normally uninfectable mouse ... A Putative Thiamine Transport Protein Is a Receptor for Feline Leukemia Virus Subgroup A. J. Virol., April 1, 2006; 80(7): 3378 ...
The protein encoded by this gene is the medium subunit of AP-3, which is an adaptor-related protein complex associated with the ... AP-3 is a heterotetrameric protein complex composed of two large subunits (delta and beta3), a medium subunit (mu3), and a ... "Entrez Gene: AP3M1 adaptor-related protein complex 3, mu 1 subunit". Human AP3M1 genome location and AP3M1 gene details page in ... 2000). "Interactions of HIV-1 nef with the mu subunits of adaptor protein complexes 1, 2, and 3: role of the dileucine-based ...
ribosomal protein S26, like. 0.013. ap3d1. adaptor-related protein complex 3, delta 1 subunit. 0.013. ... adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1. 0.031. ... BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like. 0.010. ... TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated facto. 0.038. ...
... delta both associate with the p85alpha and beta adaptor subunits and are similarly recruited to activated signaling complexes ... are lipid kinases which also possess an in vitro protein kinase activity towards themselves or their adaptor proteins. The ... and p50 adapter subunit isoforms. In addition, p85alpha-NSH2 antibodies recognized four adapter subunit variants of 54, 53, 48 ... The complex was found in larvae, pupae, and adults, consistent with p60 functioning as the adaptor for Dp110 throughout the ...
Adaptor Protein Complex delta Subunits アダプター蛋白質複合体デルタサブユニット ... GTP-Binding Protein alpha Subunits, Gq-G11 G蛋白質アルファサブユニットGq-G11 ... GTP-Binding Protein alpha Subunits, Gs G蛋白質-促進性Gs ... Hedgehog Proteins - Adaptor Proteins, Vesicular Transport 小胞輸送 ... rab GTP-Binding Proteins rab G蛋白質 ... Protein-Tyrosine Kinases Protein
Adaptor Protein Complex delta Subunits / genetics* Actions. * Search in PubMed * Search in MeSH ... protein-DNA binding; PP, protein-protein binding; RB, regulation of binding; RE, reaction; T, transcription; TR, translocation ... Anti-citrullinated peptide/protein antibody (ACPA)-negative RA shares a large proportion of susceptibility loci with ACPA- ... The cardiac syndecan-4 interactome reveals a role for syndecan-4 in nuclear translocation of muscle LIM protein (MLP). ...
adaptor-related protein complex 3, delta 1 subunit. 0.019. Mospd2. motile sperm domain containing 2. 0.019. ... protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I). 0.012. ... establishment of protein localization to vacuole. GO:0072666. The directed movement of a protein to a specific location in a ... Highlight genes with functional analogs involved in "establishment of protein localization to vacuole" (in gray). Name. ...
It shows genes and PPIs with information about pathways, protein-protein interactions (PPIs), Gene Ontology (GO) annotations ... a web resource for human protein-protein interactions. ... adaptor-related protein complex 1, gamma 1 subunit. delta/notch ... Protein-Protein Interactions. 20 interactors: AFTPH AP1AR AP1B1 AP1M2 AP1S1 AP1S2 ARF1 CLINT1 CLTC DNER KIF13A MAP1A RAB5A ... Nef-mediates down modulation of cell surface receptors by recruiting them to clathrin adapters ...
It shows genes and PPIs with information about pathways, protein-protein interactions (PPIs), Gene Ontology (GO) annotations ... a web resource for human protein-protein interactions. ... adaptor-related protein complex 3, delta 1 subunit. vacuolar ... Protein-Protein Interactions. 9 interactors: AP3S1 AP3S2 ARF1 ARF6 CTGF RUFY1 SLC30A3 VAMP7 VPS41 9 interactors: AKTIP AP3D1 ...
  • The elucidation of the genomic structure of the catalytic subunits is a necessary step for the investigation of the function of PI3K isoforms by inactivation of the gene in vivo. (embl.de)
  • PI3Kδ is a heterodimer of the p110δ catalytic subunit and the p85α, p55α, or p50α regulatory subunit and is known to play a major role in cell survival, cell growth, and cell-cycle entry through downstream mediators including AKT and mTORC1 ( 8 ). (frontiersin.org)
  • The PIK3CA gene encodes the p110alpha protein which is a catalytic subunit of the class I PI 3-kinases (PI3K). (atlasgeneticsoncology.org)
  • Class I PI3K are heterodimeric molecules composed of a catalytic subunit, a p110, and a regulatory subunit. (atlasgeneticsoncology.org)
  • PI3K s are heterodimers composed of various combinations of catalytic and regulator subunit isoforms. (diff.org)
  • Class IA PI3K heterodimers contain specific isoforms of the 85 kDa adaptor subunit that facilitates interaction with receptor tyrosine kinases (RTK) and either an alpha, beta or delta p110 catalytic subunit (p110α, p110β, or p110γ). (diff.org)
  • Class IB PI3K heterodimers contain a p101 regulatory subunit that responds to specific GPCR -associated G-protein, βγ-subunits and a gamma p110 (p110γ) catalytic subunit. (diff.org)
  • P85 contains a Src homology 3 (SH3) domain, a breakpoint-cluster region homology (BH) domain between two proline-rich regions, and two C-terminal SH2 domains separated by an inter-SH2 (iSH2) region, which tightly binds p85 to the catalytic subunit. (diff.org)
  • It is in common agreement that is actually loss of the inhibitory interaction of the p85 regulatory subunit that appears to be the basis for one common type of oncogenic mutation in the p110 γ catalytic subunit. (diff.org)
  • A recent study, based on X-ray crystal structure of the complex of the ABD with the iSH2 domain and an experimental model of the catalytic core of PI3K α, proposed a model of how the regulatory subunit would interact with the full length p110 α catalytic subunit. (diff.org)
  • 1992). It was not known at the time that agonist-stimulated PI3K is a heterodimer made up of a p110 catalytic subunit and a regulatory subunit, namely p85 in the case of class IA PI3K s and p101 in the case of the class IB p110γ. (diff.org)
  • Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. (sinobiological.com)
  • The regulatory subunit has a dual function of recruiting the p110 catalytic subunit to an activated upstream tyrosine kinase receptor, and inhibiting catalytic activity in the absence of this stimulatory interaction (Yu et al. (atlasgeneticsoncology.org)
  • Downregulation of genes encoding for subunits of adaptor complex-3 in cervical carcinomas. (nih.gov)
  • Genes encoding subunits of the endomembrane trafficking system. (elifesciences.org)
  • Subsequent cloning ofrelated genes from other organisms indicated that the candidate gene is related, but unique, to a gene family of the delta subunit of the adaptor protein (AP)complex 3, AP-3. (embl.de)
  • The myotubularin-related genes define a large family of eukaryotic proteins, most of them initially characterized by the presence of a ten-amino acid consensus sequence related to the active sites of tyrosine phosphatases, dual-specificity protein phosphatases and the lipid phosphatase PTEN. (embl.de)
  • Prolactin is a primary factor required for the growth and terminal differentiation of mammary epithelial cells as determined by the induction of transcription of milk protein genes required for lactation [ 2 ], [ 3 ]. (bio-rad.com)
  • Their stability as reference genes was validated by NormFinder, BestKeeper, geNorm and Delta Ct programs. (biomedcentral.com)
  • We also demonstrated the occurrence of multiple AP-1 complexes resulting from combinatorial assembly of various gamma (i.e., gamma1 and gamma2) and sigma1 (i.e., sigma1A, sigma1B and sigma1C) subunit isoforms encoded by different genes. (grantome.com)
  • Among the genes strongly stimulated by DHT in C4-2B cells - D-dopachrome tautomerase (DDT), Protein kinase C delta (PRKCD), Glutathione S- transferase theta 2 (GSTT2), Transient receptor potential cation channel subfamily V member 3 (TRPV3), and Pyrroline-5-carboxylate reductase 1 (PYCR1) - most were less strongly or hardly stimulated in LNCaP cells. (biomedcentral.com)
  • It's been demonstrated that CRL4B mediates the ubiquitination and following degradation of WDR5, a primary subunit of histone H3 lysine 4 (H3K4) methyltransferase complexes, therefore regulating the manifestation of neuronal genes 25. (barasertib.info)
  • Activated PI3Kδ Syndrome (APDS) is an inherited immune disorder caused by heterozygous, gain-of-function mutations in the genes encoding the phosphoinositide 3-kinase delta (PI3Kδ) subunits p110δ or p85δ. (frontiersin.org)
  • Ten genes associated with HPS are identified to date, and each gene encodes a protein subunit of either Biogenesis of Lysosome-related Organelles Complex (BLOC)-1, BLOC-2, BLOC-3, or the Adaptor Protein-3 complex. (bvsalud.org)
  • This axon genes the results reviewed by SLC outliers, by which complexes and families, not as as members, porphyrias, linkages, and homozygous musicians are beta T ligases( He et al. (evakoch.com)
  • Each multidomain thrombopoiesis is a breast of 7 transcriptional co-chaperone genes in two proteins, the city and neuronal cells. (evakoch.com)
  • These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. (exbio.cz)
  • Class I PI3K regulatory subunits are derived from three genes. (diff.org)
  • Within this group, sarcoglycanopathies are a subgroup caused by mutations in genes encoding the transmembrane protein sarcoglycan (SG) complex, located in the sarcolemma of striated muscles 2 . (nature.com)
  • Because the SG complex plays a key role in the maintenance of sarcolemma integrity during muscle contraction, mutations in these genes lead to muscular dysfunction 3 . (nature.com)
  • The authors have recently described an interaction between Gag and the delta subunit of the AP-3 adaptor protein complex (1). (indigo.ca)
  • Disruption of the interaction, using a specific piece of the AP-3 subunit, eliminated Gag trafficking to the multivesicular body and diminished HIV particle formation in cells. (innovations-report.com)
  • Gibbon ape leukemia virus (GALV) enters cells following interaction with a specific receptor protein. (aacrjournals.org)
  • Comparison of the structural features of these domains not only reveals a high degree of conservation of their lipid interaction sites but also highlights their evolutionary link to protein modules known for protein-protein interactions. (biologists.org)
  • For soluble proteins, luminal pH and divalent metals can affect aggregation and interaction with surrounding membranes. (frontiersin.org)
  • Although the bovine adaptor protein 3 complex subunit delta-1 (boAP3D1) has been proposed as the potential receptor, the specific ligand-receptor interaction has not yet been completely defined and boAP3D1 receptor and gp51 3D structures have not been determined. (urosario.edu.co)
  • This study was thus aimed at a functional annotation of boAP3D1 cellular adaptor protein and BLV gp51 and, proposing a reliable model for gp51-AP3D1 interaction using bioinformatics tools. (urosario.edu.co)
  • The boAP3D1 receptor interaction patterns were calculated based on models of boAP3D1 receptor and gp51 complexes' 3D structures, which were constructed using homology techniques and data-driven docking strategy. (urosario.edu.co)
  • These results demonstrate that APP and AP-4 engage in a novel type of signal-adaptor interaction that mediates transport of APP from the trans-Golgi network to endosomes, thereby reducing amyloidogenic processing of the protein. (grantome.com)
  • One such protein is p21Cip1, which inhibits DNA replication through its interaction with PCNA, while allowing repair to continue. (sdbonline.org)
  • Hinge and ear domains are important to the interaction with coat proteins and regulatory/accessory proteins. (bioscirep.org)
  • also called δ-catenin) binds via a postsynaptic density-95 (PSD-95)/discs large/zona occludens-1 (PDZ) interaction to AMPA receptor (AMPAR)-binding protein (ABP) and the related glutamate receptor (GluR)-interacting protein (GRIP), two multi-PDZ proteins that bind the GluR2 and GluR3 AMPAR subunits. (jneurosci.org)
  • Exogenous NPRAP that was bound to cadherins at adherens junctions of Madin-Darby canine kidney cells recruited ABP from the cytosol to form cadherin-NPRAP-ABP complexes, dependent on NPRAP interaction with the ABP PDZ domain 2. (jneurosci.org)
  • In cultured hippocampal neurons, dominant-negative mutants of NPRAP designed to disrupt tethering of ABP to NPRAP-cadherin complexes reduced surface levels of endogenous GluR2, indicating that interaction with cadherin-NPRAP-ABP complexes stabilized GluR2 at the neuronal plasma membrane. (jneurosci.org)
  • Thus, the interaction of scaffolding proteins with cadherin-NPRAP complexes may anchor diverse signaling and adhesion molecules at cadherins. (jneurosci.org)
  • In the absence of a WNT-Frizzled low density lipoprotein receptor-related protein-5/6 co-receptor interaction (WNT-FZD-LRP5/6), β-catenin is caught in a molecular destruction complex, phosphorylated and degraded by the proteasome. (biomedcentral.com)
  • Upon WNT-receptor interaction, the destruction complex is disassembled, β-catenin accumulates in the cell, translocates to the nucleus and associates with transcription factors of the T-cell factor/lymphoid enhancer factor (TCF/LEF) family. (biomedcentral.com)
  • Since PI3K has multiple protein-interaction domains, p85 is able to interact with several signaling molecules simultaneously, allowing for significant fine tuning of PI3K activity. (diff.org)
  • Protein interaction domains can be classified based on sequence homology, ligand-binding properties, or structural similarity [ 11 ]. (hindawi.com)
  • Thus, a typical class of ligand binding proteins may contain a variety of protein interaction domains that recognize a common ligand, whereas a family classified based on sequence homology contains a single fold that may recognize a variety of ligands. (hindawi.com)
  • DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. (antikoerper-online.de)
  • Right here, we record that dBRWD3, the take flight homolog of XLID proteins BRWD3, adversely regulates the quantity of H3.3 connected with YEM as well as the?degrees of chromatin-associated H3.3. (barasertib.info)
  • MLH1 (mutL homolog) is a protein with sequence similarity to DNA mismatch repair proteins of bacteria (MutL and HexB) and S. cerevisiae yeast (PMS1). (sdbonline.org)
  • MSH2 is a homolog of the bacterial DNA mismatch protein MutS. (sdbonline.org)
  • Following IL-5 -induced hetero-dimerization, IL-5 receptor binds to multiple signal-transducing proteins, which include Janus kinase 1 ( JAK1 ) and 2 ( JAK2 ), v-yes-1 Yamaguchi sarcoma viral related oncogene homolog ( Lyn ), spleen tyrosine kinase ( Syk ) and SHC transforming protein 1 ( Shc ). (bio-rad.com)
  • . SOS promotes v-Ha-ras Harvey rat sarcoma viral oncogene homolog ( H-Ras )/ v-Raf-1 murine leukemia viral oncogene homolog 1 ( c-Raf-1 )/ Mitogen-activated protein kinase kinase 1 and 2 ( MEK1 and MEK2 )/ ERK1/2 pathway [ 24 ], [ 25 ]. (bio-rad.com)
  • Through a series of experiments, the group demonstrated that the AP-3 delta subunit interacts with Gag to direct it to the multivesicular body. (innovations-report.com)
  • AP-1A recruitment to membranes can be modulated by Phosphofurin Acidic Cluster Sorting protein 1 (PACS-1), a cytosolic protein which interacts with both AP-1A and cargo that has been phosphorylated by casein kinase II. (frontiersin.org)
  • Through its adaptor binding domain p110alpha interacts with the regulatory subunit. (atlasgeneticsoncology.org)
  • U6atac snRNA stem-loop interacts with U12 p65 RNA binding protein and is functionally interchangeable with the U12 apical stem-loop III. (mpg.de)
  • At the postsynaptic side, the scaffolding protein postsynaptic density-95 (PSD-95) binds neuroligin (for review, see Kim and Sheng, 2004 ), a postsynaptic CAM that interacts with presynaptic neurexins. (jneurosci.org)
  • Because we find that NPRAP also binds to PSD-95, cadherin-NPRAP complexes may be linked to the PSD-95 scaffold, which is involved in tethering NMDAR and TARP-AMPAR complexes and which also interacts with neuroligins. (jneurosci.org)
  • Of note, the SH3 domain interacts with Src (Src homology 2/α-collagen-related), CDC42GAP (Cdc42 GTP ase-activating protein) and the proto-oncogene product Cbl. (diff.org)
  • Interacts with herpes simplex virus 1 protein UL46. (sinobiological.com)
  • 14-3-3 beta / YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. (sinobiological.com)
  • p110delta interacts with its associated p85 regulatory subunit via the adaptor binding domain (ABD). (atlasgeneticsoncology.org)
  • The classical effector of PI3K signalling is the serine/threonin kinase AKT (aka protein kinase B), which contains a pleckstrin homology (PH) domain that interacts with PIP 3 (Franke et al. (atlasgeneticsoncology.org)
  • The protein encoded by this gene is the medium subunit of AP-3, which is an adaptor-related protein complex associated with the Golgi region as well as more peripheral intracellular structures. (nih.gov)
  • AP-3 is a heterotetrameric protein complex composed of two large subunits (delta and beta3), a medium subunit (mu3), and a small subunit (sigma 3). (nih.gov)
  • A gene on chromosome 5q14.1 that encodes a subunit of the heterotetrameric AP3 complex, a protein complex composed of two large subunits (delta and beta), a medium subunit (mu), and a small subunit (sigma). (thefreedictionary.com)
  • This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. (antikoerper-online.de)
  • Among these adaptor proteins are the heterotetrameric AP-1, AP-2, AP-3 and AP-4 complexes, the monomeric GGA proteins, and the heteropentameric retromer complex. (grantome.com)
  • The AP (adaptor protein) complexes are heterotetrameric protein complexes that mediate intracellular membrane trafficking along endocytic and secretory transport pathways. (bioscirep.org)
  • A ) Diagrams of heterotetrameric AP complexes. (bioscirep.org)
  • Interactions of viral protein U (Vpu) with cellular factors. (indigo.ca)
  • Imaging of HIV/host protein interactions. (indigo.ca)
  • Vpr and its interactions with cellular proteins. (indigo.ca)
  • V1H can function as an adaptor for interactions between Nef and AP-2 (zeige GTF3A ELISA Kits ). (antikoerper-online.de)
  • Protein-protein interactions involving lipid-binding domains could serve as the basis for phosphoinositide-induced conformational regulation of target proteins at biological membranes. (biologists.org)
  • Here, I focus on the structural and functional similarities between these modules, emphasising their proven or potential protein-protein interactions in combination with their lipid-binding properties. (biologists.org)
  • The location and topography of the corresponding sites are similar, although the strength and amino acid requirements of different interactions depend on the exact sequence of the signal and the particular AP complex involved. (grantome.com)
  • Because these kinases exhibit constitutive activity in biochemical assays, it is likely that their activity in cells is controlled by subcellular localization, interactions with inhibitory proteins, targeted degradation, or combinations of these mechanisms. (sciencemag.org)
  • It mediates protein-protein interactions by binding to proline-rich motifs in target proteins forming multimeric signaling complexes. (diff.org)
  • T. Itoh established a comprehensive system to examine two-hybrid interactions in all possible combinations between the yeast S, cerevisiae proteins, and predicted several gene cascades base on the identified interactions. (nii.ac.jp)
  • The current version of the human immunome network consists of nearly 1400 interactions involving approximately 600 proteins. (hindawi.com)
  • Protein-protein interactions (PPIs) are critical for most biological functions and cellular processes [ 1 , 2 ]. (hindawi.com)
  • the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). (uniprot.org)
  • Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. (ptglab.com)
  • Results indicate that AP-2 is not essential for clathrin -coated vesicle formation at the plasma membrane, but that it is one of several endocytic adaptors required for the uptake of certain cargo proteins. (antikoerper-online.de)
  • Part of the AP-3 complex, an adaptor-related complex which is essential for the compartmentalization of the endocytic pathway. (mybiosource.com)
  • Aberrant endocytic processing through disruption of adaptor protein complexes is likely to result from the AP1S2 mutations identified in the three XLMR-affected families, and such defects may plausibly cause abnormal synaptic development and function. (ox.ac.uk)
  • AP1S2 is the first reported XLMR gene that encodes a protein directly involved in the assembly of endocytic vesicles. (ox.ac.uk)
  • The Rab5 proteins are known to regulate membrane docking and fusion in the early endocytic pathway. (jove.com)
  • In the endocytic and secretory pathways, cargo proteins destined for transport to distinct locations are collectively assembled into vesicles and delivered to their target sites by vesicular trafficking. (bioscirep.org)
  • Epsin is an endocytic protein that binds Clathrin, the plasma membrane, Ubiquitin, and also a variety of other endocytic proteins through well-characterized motifs. (stanford.edu)
  • AP3D1 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). (ptglab.com)
  • Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2 ), a medium adaptin (mu-type subunit AP3M1 or AP3M2 ) and a small adaptin (sigma-type subunit APS1 or AP3S2 ) (By similarity). (rcsb.org)
  • This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. (antikoerper-online.de)
  • The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). (antikoerper-online.de)
  • We identify dynamin and the EAP -binding alpha-adaptin appendage domain of the AP2 adaptor as switches in a regulated, multistep maturation process and provide direct evidence for a molecular checkpoint in clathrin mediated endocytosis. (antikoerper-online.de)
  • Also known as AP-3 complex subunit beta (Adaptor protein complex AP-3 beta subunit) (Adaptor-related protein complex 3 subunit beta) (Beta-3-adaptin) (Clathrin assembly protein complex 3 beta large chain). (mybiosource.com)
  • Also known as AP-3 complex subunit delta (Adaptor-related protein complex 3 subunit delta) (Delta-adaptin 3) (Delta-adaptin). (mybiosource.com)
  • Also known as AP-3 complex subunit delta-1 (AP-3 complex subunit delta) (Adaptor-related protein complex 3 subunit delta-1) (BLVPCP1) (Bovine leukemia virus cell receptor) (BLV-R) (Delta-adaptin). (mybiosource.com)
  • Also known as AP-3 complex subunit mu (Adaptor protein complex AP-3 subunit mu) (Adaptor protein-3 mu-adaptin) (Adaptor-related protein complex 3 subunit mu) (At-muD-Ad) (Mu3-adaptin) (Protein ZIG SUPPRESSOR 4). (mybiosource.com)
  • AP1S2 encodes an adaptin protein that constitutes part of the adaptor protein complex found at the cytoplasmic face of coated vesicles located at the Golgi complex. (ox.ac.uk)
  • Evaluation of the delta subunit of bovine adaptor protein complex 3 as a receptorfor bovine leukaemia virus. (embl.de)
  • Component of the coatomer, a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. (uniprot.org)
  • The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. (uniprot.org)
  • The trafficking of granule membrane proteins can be controlled by both luminal and cytosolic factors. (frontiersin.org)
  • Cytosolic adaptor proteins (APs), which recognize the cytosolic domains of proteins that span the SG membrane, have been shown to play essential roles in the assembly of functional SGs. (frontiersin.org)
  • Membrane proteins: Transmembrane proteins which reside in the ER contain sorting signals in their cytosolic tails which direct the protein to exit the Golgi and return to the ER. (wikipedia.org)
  • Sorting is mediated by recognition of signals present in the cytosolic domains of the transmembrane proteins by adaptor proteins that are components of membrane coats (e.g., clathrin coats). (grantome.com)
  • We recently found that, instead, the mu4 subunit of AP-4 binds an YKFFE sequence from the cytosolic tail of the Alzheimer's disease amyloid precursor protein (APP). (grantome.com)
  • The ArfGAP family of cytosolic proteins is characterized by a well-conserved catalytical zinc finger domain, whereas their noncatalytical domains differ between subgroups of the family ( Randazzo and Hirsch, 2004 ). (rupress.org)
  • The protein encoded by this gene is a subunit of the AP3 adaptor-like complex, which is not clathrin-associated, but is associated with the golgi region, as well as more peripheral structures. (genecards.org)
  • The AP-3 complex facilitates the budding of vesicles from the golgi membrane, and may be directly involved in trafficking to lysosomes. (genecards.org)
  • The complex is associated with the Golgi region as well as more peripheral structures. (genecards.org)
  • AP-3 facilitates the budding of vesicles from the Golgi membrane, and it may directly function in protein sorting to the endosomal/lysosomal system. (nih.gov)
  • The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. (uniprot.org)
  • AP3 plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes, as well as in biogenesis of melanosomes, platelet dense granules and lysosomes. (thefreedictionary.com)
  • AP3 plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes by facilitating budding of vesicles from the Golgi membrane. (thefreedictionary.com)
  • Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. (uniprot.org)
  • COPI is a coatomer , a protein complex [1] that coats vesicles transporting proteins from the cis end of the Golgi complex back to the rough endoplasmic reticulum (ER), where they were originally synthesized , and between Golgi compartments. (wikipedia.org)
  • The name "COPI" refers to the specific coat protein complex that initiates the budding process on the cis -Golgi membrane. (wikipedia.org)
  • Luminal proteins: Proteins found in the lumen of the Golgi complex that need to be transported to the lumen of the ER contain the signal peptide KDEL . (wikipedia.org)
  • In previous work, we found that a complex named GARP is a critical component of the molecular machinery that mediates retrograde transport of various cargo proteins, including sorting receptors, processing endopeptidases, fusogenic proteins and bacterial and plant toxins, from endosomes to the trans-Golgi network in mammalian cells. (grantome.com)
  • We showed that the molecular function of this complex is to promote tethering and fusion of endosome-derived transport carriers to the trans-Golgi network. (grantome.com)
  • Interference with any of the GARP subunits blocks the delivery of many cargos to the trans-Golgi network, leading to global defects in lysosomal function, lipid traffic and autophagy. (grantome.com)
  • ArfGAP1 is a prototype of GTPase-activating proteins for ADP-ribosylation factors (ARFs) and has been proposed to be involved in retrograde transport from the Golgi apparatus to the endoplasmic reticulum (ER) by regulating the uncoating of coat protein I (COPI)-coated vesicles. (genes2cognition.org)
  • Furthermore, in these cells proteins resident in or cycling through the cis-Golgi, including ERGIC-53, beta-COP, and GM130, accumulate in the ER-Golgi intermediate compartment, and Golgi-to-ER retrograde transport is blocked. (genes2cognition.org)
  • The formation of coat protein complex I (COPI)-coated vesicles is regulated by the small guanosine triphosphatase (GTPase) adenosine diphosphate ribosylation factor 1 (Arf1), which in its GTP-bound form recruits coatomer to the Golgi membrane. (genes2cognition.org)
  • Second, PI4KII also has an unforeseen function in late endosomes, where it is required for normal retromer dynamics and for formation of tubular endosomes that are likely to be involved in retrieving Snap24 and Lysosomal enzyme receptor protein (Lerp) from late endosomes to the trans-Golgi network. (biologists.org)
  • CACNG3 (Calcium Voltage-Gated Channel Auxiliary Subunit Gamma 3) is a Protein Coding gene. (genecards.org)
  • Previous studies showed that tyrosine-based signals bind to the mu1, mu2 and mu3 subunits, whereas dileucine-based signals bind to a combination (i.e., a hemicomplex) of two subunits, gamma-sigma1, alpha-sigma2 and delta-sigma3, from the corresponding AP complexes. (grantome.com)
  • Association of methotrexate treatment with a decrease of double negative (CD4-CD8-) and gamma/delta T cell levels in patients with juvenile rheumatoid arthritis. (faintpower.ga)
  • The antibody MEM-57 reacts with gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes. (exbio.cz)
  • it reacts strongly with gamma/delta T lymphocytes. (exbio.cz)
  • T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. (exbio.cz)
  • Dave VP, Cao Z, Browne C, Alarcon B, Fernandez-Miguel G, Lafaille J, de la Hera A, Tonegawa S, Kappes DJ: CD3 delta deficiency arrests development of the alpha beta but not the gamma delta T cell lineage. (exbio.cz)
  • Heterotrimeric G proteins, consisting of alpha, beta and gamma subunits, couple ligand-bound seven transmembrane domain receptors (GPCRs or G-protein coupled receptors) to the regulation of effector proteins and production of intracellular secondmessengers such as cAMP, cGMP, and Ca.sup.2+. (patentgenius.com)
  • Does not show subunit-specific AMPA receptor regulation and regulates all AMPAR subunits. (genecards.org)
  • Arkadia (zeige RNF111 ELISA Kits ) complexes with clathrin adaptor AP2 (zeige GTF3A ELISA Kits ) mu2 subunit and regulates EGF (zeige EGF ELISA Kits ) signalling. (antikoerper-online.de)
  • We find that the E3 ubiquitin ligase complex Cullin1(Cul1)/SkpA/Supernumerary limbs(Slimb) regulates the stability of one of the peripheral membrane components, Prickle (Pk). (stanford.edu)
  • 14-3-3 beta / YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation. (sinobiological.com)
  • The protein encoded by this gene is a type I transmembrane AMPA receptor regulatory protein (TARP). (genecards.org)
  • Class IA PI3K s are tightly associated with a 85 kDa regulatory subunit called p85. (diff.org)
  • 1998). Unlike p110alpha, p110delta does not phoshorylate its associated p85 regulatory subunit and instead auto-phosphorylates (Vanhaesebroeck et al. (atlasgeneticsoncology.org)
  • AP-3 is an "adaptor protein complex," a group of four proteins known to sort cargo proteins to specific compartments in the cell. (innovations-report.com)
  • Adaptor protein 1A (AP-1A) is known to interact with specific motifs in its cargo proteins and with the clathrin heavy chain, contributing to the formation of a clathrin coat. (frontiersin.org)
  • A cargo/PACS-1/AP-1A complex is necessary to drive the appropriate transport of several cargo proteins within the regulated secretory pathway. (frontiersin.org)
  • We review the functions of AP-1A, PACS-1, and GGAs in facilitating the retrieval of proteins from immature SGs and review examples of cargo proteins whose trafficking within the regulated secretory pathway is governed by APs. (frontiersin.org)
  • Required for the transport via the ALP pathway, which directs the transport of the cargo proteins PHO8 and VAM3 to the vacuole. (mybiosource.com)
  • The primary function of adaptors is the selection of cargo proteins for their incorporation into nascent carriers. (wikipedia.org)
  • [10] [9] The order in which adaptor proteins associate with cargo, or adaptor proteins associate with ARFs is unclear, however, in order to form a mature transport carrier coat protein, adaptor, cargo, and ARF must all associate. (wikipedia.org)
  • The β′- and α-COP subunits form an arch over the γζβδ-COP subcomplex, orienting their N-terminal domains such that the K(X)KXX cargo-motif binding sites are optimally positioned against the membrane. (wikipedia.org)
  • Based on our work, we hypothesize that defects in these diseases are caused by abnormal sorting of specific cargo proteins having dileucine-based signals. (grantome.com)
  • BICD cargo adaptor 1 [Source:HGNC Symb. (broadinstitute.org)
  • Arf GTPase-activating protein (GAP) catalyzed GTP hydrolysis in Arf1 triggers uncoating and is required for uptake of cargo molecules into vesicles. (genes2cognition.org)
  • They recognize and concentrate cargo proteins into vesicular carriers that mediate transport from a donor membrane to a target organellar membrane. (bioscirep.org)
  • They bind to sorting signals in the cytoplasmic tails of cargo proteins, recruit clathrin and other accessory proteins, and then concentrate the cargo proteins into vesicular carriers, which transport from the donor membrane to the target organelle membrane ( Figure 1 ). (bioscirep.org)
  • Core domains are responsible for the cargo protein binding and membrane localization. (bioscirep.org)
  • In the locked state, the cargo-binding sites of AP-1 are hindered by two large subunits (γ and β1). (bioscirep.org)
  • In the open state, both [D/E]XXXL[L/I] and YXXØ-binding sites are exposed and ready to bind to corresponding cargo proteins (yellowish circles). (bioscirep.org)
  • In this Topical Review, we present their main protein components and discuss current models for cargo sorting. (deepdyve.com)
  • It is known that mannose binding protein can mediate the adhesion of Acanthamoeba to the surface of cornea [ 5 ]. (parasitol.kr)
  • The G1 subunits of SHC1 is However achieved modified with FGFR3, but this is first of Aldosterone process, and the cohesin-bound development now branched involved to mediate other activity. (evakoch.com)
  • These five AP complexes localize to different intracellular compartments and mediate membrane trafficking in distinct pathways. (bioscirep.org)
  • Moelleken, J. 2006-10-14 00:00:00 COPI-coated vesicles are protein and liquid carriers that mediate transport within the early secretory pathway. (deepdyve.com)
  • YWHAB gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. (sinobiological.com)
  • This gene encodes a protein which is similar to the Drosophila crumbs protein and localizes to the inner segment of mammalian photoreceptors. (antibodies-online.com)
  • This subunit is implicated in intracellular biogenesis and trafficking of pigment granules, and possibly platelet dense granules and neurotransmitter vesicles. (genecards.org)
  • In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals (By similarity). (genecards.org)
  • The assembly of AP-3 adaptor complex-containing clathrin-coated vesicles on synthetic liposomes. (nih.gov)
  • Gag is a myristoylated precursor protein that is translated in the cytoplasm and then traffics to the plasma membrane or to endosomal vesicles for assembly. (indigo.ca)
  • The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. (antikoerper-online.de)
  • This family consists of several bovine specific leukaemia virus receptors which are thought to function as transmembrane proteins, although their exact function is unknown (PMID:12692298). (embl.de)
  • Alpha-subunit is ligand-specific and beta subunit is shared with the receptors for Interleukin 3 (IL-3) and granulocyte-macrophage colony-stimulating-factor (GM-CSF) [ 3 ], [ 4 ]. (bio-rad.com)
  • Ephrin including is abrogated to Rho download Fashion, costume, and culture: clothing, headwear, body decorations, and footwear receptors IP7 as Rac, Rho and Cdc42 that show 47S epithelia proteins to knockouts in the pol activation( Noren & Pasquale 2004, Groeger & Nobes 2007). (erik-mill.de)
  • NPRAP also binds PSD-95, which is a scaffold for NMDA receptors, for AMPARs in complexes with auxiliary subunits, the TARPs (transmembrane AMPA receptor regulator proteins), and for adhesion molecules. (jneurosci.org)
  • In the nerve terminal, scaffolding proteins bound to CAM intracellular domains tether the neurotransmitter release machinery, whereas postsynaptic scaffolding proteins, also bound to CAMs, anchor glutamate receptors. (jneurosci.org)
  • G protein-coupled cell surface receptors (GPCRs) have served a fundamental role in modern pharmacology, due to their central importance in cell communication, and have been the target for the discovery of a large number of drugs. (ddw-online.com)
  • sub.q protein variants that functionally couple to sensory cell receptors such as taste GPCRs (TRs) and olfactory GPCRs (ORs) in an overly promiscuous manner. (patentgenius.com)
  • This invention relates to G.alpha.q protein variants and their use in the analysis and discovery of agonists and antagonsists of chemosensory receptors, such as G protein coupled receptors involved in sensing of tastants, olfactants, andpheromones. (patentgenius.com)
  • Agonist-bound sensory receptors catalyze theexchange of GTP for GDP on the surface of the G.alpha.a subunit to initiate intracellular responses to extracellular signals. (patentgenius.com)
  • For instance, the FGF receptor and TrkA receptors activate PI3 -kinase through the FRS -2:Grb2:Gab1 complex. (diff.org)
  • His work has revealed key functional and structural information about T cell receptors (TCRs), the CD3 signaling subunits, and how, with other molecules, TCRs bind to peptide-loaded MHC. (dana-farber.org)
  • This protein in turn binds to the ARF. (wikipedia.org)
  • During a clamp loading circle, the RFC-clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. (string-db.org)
  • NPRAP is an ARM repeat protein that binds to the juxtamembrane region of the cadherin intracellular domain. (jneurosci.org)
  • MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. (string-db.org)
  • Expression levels of proteins secreted into extracellular space were compared between A. castellanii pathogenic (ACP) and non-pathogenic strains. (parasitol.kr)
  • Proteins secreted into extracellular space as potential targets for treatment and diagnosis of amoebiasis have been reported [ 7 , 8 ]. (parasitol.kr)
  • To gain more information on virulence factors of Acanthamoeba infection, the objective of this study was to compare the levels of proteins secreted into extracellular space between pathogenic ATCC 30011 strain and non-pathogenic ATCC 30868 strain of Acanthamoeba . (parasitol.kr)
  • Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. (antikoerper-online.de)
  • Both subunits contain extracellular domains. (bio-rad.com)
  • 3 4 5 6 7 Retinoschisin is almost entirely composed of a highly conserved discoidin domain frequently found in a wide range of membrane and extracellular proteins and most likely mediating cell adhesion and cellular signaling processes. (arvojournals.org)
  • The coat consists of large protein subcomplexes that are made of seven different protein subunits, namely α, β, β', γ, δ , ε and ζ . (wikipedia.org)
  • Coat protein, or COPI, is an ADP ribosylation factor (ARF)-dependent protein involved in membrane traffic. (wikipedia.org)
  • Current work in our laboratory is aimed at elucidating the structure, regulation and physiological roles of coat proteins and vesicle tethering factors, and investigating human diseases that result from genetic defects (e.g. (grantome.com)
  • Three homologous ArfGAPs participate in coat protein I-mediated transport. (genes2cognition.org)
  • We suggest that ArfGAP2 and ArfGAP3 are coat protein-dependent ArfGAPs, whereas ArfGAP1 has a more general function. (genes2cognition.org)
  • Recently, proteomic analysis of secreted proteins by Acanthamoeba for non-pathogenic strain ATCC 30010 and clinically pathogenic isolates has been performed [ 13 ], revealing 44 secreted proteins, including 10 consensus secretory proteins and 34 strain specific secretory proteins. (parasitol.kr)
  • These professional secretory cells devote as much as half of their total protein synthesis to the production of a single hormone ( 2 ). (frontiersin.org)
  • Our genetic analysis of PI4KII in flies thus reveals a novel role for PI4KII in regulating the fidelity of granule protein trafficking in secretory tissues. (biologists.org)
  • These sorting signals, or motifs, typically contain the amino acid sequence KKXX or KXKXX, which interact with COPI subunits α-COP and β'-COP. (wikipedia.org)
  • This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. (sinobiological.com)
  • Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. (sinobiological.com)
  • One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. (abnova.com)
  • More recent studies on correctors of the misfolded ∆F508 mutant protein in the cystic fibrosis transmembrane conductance regulator (CFTR) have described their positive impact on α-SG missense mutant protein localization in the plasma membrane in primary muscle cells from patients 16 . (nature.com)
  • Many small insertions and deletionsof nucleotides could generate the predicted transmembrane and cytoplasmic regionsof the BLVR protein from the prototypic boAP3delta gene. (embl.de)
  • CD2 cytoplasmic tail binding protein 2. (broadinstitute.org)
  • stability membranes see an critical other protein through which they contain to type houses, a small industry alpha, and an hematopoietic cytoplasmic containing serine entering a alpha-D-glucose application Pyruvate c1 half-life also together as biochemical asparagine absence mammals. (erik-mill.de)
  • Antigen standard for adaptor-related protein complex 2, sigma 1 subunit (AP2S1), transcript variant AP17delta is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. (creativebiomart.net)
  • Mutations in the gene encoding the Sigma 2 subunit of the adaptor protein 1 complex, AP1S2, cause X-linked mental retardation. (ox.ac.uk)
  • Adaptor protein complexes AP-1 and AP-3 are required by the HHV-7 Immunoevasin U21 for rerouting of class I MHC molecules to the lysosomal compartment. (nih.gov)
  • Altered trafficking of lysosomal proteins in Hermansky-Pudlak syndrome due to mutations in the beta 3A subunit of the AP-3 adaptor. (nih.gov)
  • The production of mature SGs requires concentrating newly synthesized soluble content proteins in granules whose membranes contain the appropriate integral membrane proteins. (frontiersin.org)
  • The mechanisms underlying the sorting of soluble and integral membrane proteins destined for SGs from other proteins are not yet well understood. (frontiersin.org)
  • High-resolution NMR determination of the dynamic structure of membrane proteins. (mpg.de)
  • p110 delta localises to the cytosol and is recruited to the periplasmic region upon stimulation of upstream activators. (atlasgeneticsoncology.org)
  • Rasl and a putative guanine nucleotide exchange factor perform crucial steps in signaling by the sevenless protein tyrosine kinase. (springer.com)
  • Basler K, Hafen E. Control of photoreceptor cell fate by the sevenless protein requires a functional tyrosine kinase domain. (springer.com)
  • Although myotubularin was thought to be a dual-specificity protein phosphatase, recent results indicate that it is primarily a lipid phosphatase, acting on phosphatidylinositol 3-monophosphate, and might be involved in the regulation of phosphatidylinositol 3-kinase (PI 3-kinase) pathway and membrane trafficking. (embl.de)
  • Phosphatidylinositol 3-kinases are a family of dual specificity lipid/protein kinases. (embl.de)
  • Moreover, recruitment of multiple proteins containing PH domains binding to the same inositol lipid would create a large degree of information `spreading' and it is hard to see how cells could maintain signaling specificity if this were the case. (biologists.org)
  • To clarify the molecular basis of FEN-1 specificity and PCNA activation, structures of FEN-1:DNA and PCNA:FEN-1-peptide complexes, along with fluorescence resonance energy transfer (FRET) and mutational results are reported. (sdbonline.org)
  • Experiments in which DUF1669 domains were swapped among FAM83 family members suggested that DUF1669 determines the specificity of the FAM83 protein for particular CK1 isoforms. (sciencemag.org)
  • subunits also contribute to the specificity of receptor-G protein coupling (Xuet al. (patentgenius.com)
  • Mutations in one of the large subunits of AP-3 have been associated with the Hermansky-Pudlak syndrome, a genetic disorder characterized by defective lysosome-related organelles. (nih.gov)
  • also studied ClpC proteins that had mutations to the middle domain that prevented the repressed state from forming. (elifesciences.org)
  • However, while there are no frequent hotspot mutations within the Ras-binding domain (RBD), hotspot mutations within the adaptor-binding domain (ABD) and the C2 domain are also frequently found. (atlasgeneticsoncology.org)
  • AAA+ ( A TPase a ssociated with a variety of cellular a ctivities) proteins control a multitude of essential cellular activities by converting the energy derived from ATP hydrolysis into a mechanical force to remodel bound substrates ( Hanson and Whiteheart, 2005 ). (elifesciences.org)
  • The PI(3,4,)P3 provokes the recruitment to cellular membranes of a variety of signalling proteins, containing PX domain, pleckstrin homology domains (PH domains), FYVE domains and other phosphoinositide-binding domains. (atlasgeneticsoncology.org)
  • Information on protein localization on the subcellular level is important to map and characterize the proteome and to better understand cellular functions of proteins. (genes2cognition.org)
  • FAM83 proteins participate in various cellular processes and are characterized by an N-terminal "domain of unknown function" called DUF1669. (sciencemag.org)
  • Members of the casein kinase 1 (CK1) family of serine-threonine protein kinases are implicated in the regulation of many cellular processes, including the cell cycle, circadian rhythms, and Wnt and Hedgehog signaling. (sciencemag.org)
  • Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. (string-db.org)
  • 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. (sinobiological.com)
  • The Class IA phosphoinositide 3-kinase delta (PI3Kδ) complex is recruited to phosphotyrosines and catalyzes the phosphorylation of phosphatidylinositol-4,5-bisphosphate to generate phosphatidylinositol-(3,4,5)-trisphosphate (PIP 3 ) that acts as a second messenger recruiting downstream signaling molecules. (frontiersin.org)
  • 100%). To determine whether dBRWD3 features like a subunit from the CRL4 complicated in these procedures, we complemented mutant clones with either wild-type dBRWD3 or delta-N-dBRWD3 mutant proteins that cannot bind towards the DDB1 adaptor from the CLR4 complicated 24. (barasertib.info)
  • The ability of PCNA to bind to MLH1 and MSH2 may reflect linkage between mismatch repair and replication and may be relevant to the roles of mismatch repair proteins in other DNA transactions (Umar, 1996). (sdbonline.org)
  • In the repressed state ClpC cannot interact with its partner peptidase and the shape of the assembly shields the sites where adapter proteins can bind. (elifesciences.org)
  • p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later. (uniprot.org)
  • As in other multicellular organisms, RAS proteins play a key role in the signaling pathways that regulate cell growth and differentiation in Drosophila . (springer.com)
  • They are key players in protein quality control by targeting misfolded and aggregated proteins to degrading and refolding pathways. (elifesciences.org)
  • This encoded protein is a calcium-dependent serine endoprotease that can efficiently cleave precursor proteins at their paired basic amino acid processing sites. (antikoerper-online.de)
  • Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. (string-db.org)
  • Until the complex thesis remains thus 15 homologs predominantly, the SLX4 Circulating regulation cleaves not intracellular. (evakoch.com)
  • Cryo-EM of mitotic checkpoint complex-bound APC/C reveals reciprocal and conformational regulation of ubiquitin ligation. (mpg.de)
  • Regulation of thymocyte trafficking by Tagap, a GAP domain protein linked to human autoimmunity. (dana-farber.org)
  • The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. (antikoerper-online.de)
  • The adaptor MecA activates ClpC by targeting substrates and stimulating ClpC ATPase activity. (elifesciences.org)
  • IL-5 receptor is composed of two polypeptide chains: alpha and beta subunits. (bio-rad.com)
  • 1. A nucleic acid encoding a mutated Gq protein identical to the polypeptide contained in SEQ ID NO:1 or 37 except for the substitution of an aspartic acid residue for theglycine residue at position 66. (patentgenius.com)
  • 6. A nucleic acid encoding a mutated Gq protein which is identical to the polypeptide contained in SEQ ID NO:1 except for the replacement of the last 5 amino acid residues with the 5 amino acid residues of the carboxy terminus of Golf ortransducin. (patentgenius.com)
  • Search, Find and Buy Antibodies, ELISA Kits and Proteins. (antibodies-online.com)
  • 171 Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) Antibodies from 23 manufacturers are available on www.antibodies-online.com. (antibodies-online.com)
  • Custom ELISA Kits, Recombinant Proteins and Antibodies can be designed, manufactured and produced according to the researcher's specifications. (mybiosource.com)
  • Here we report on a pilot study of 466 proteins in three human cell lines aimed to allow large scale confocal microscopy analysis using protein-specific antibodies. (genes2cognition.org)
  • This is the first large scale antibody-based study to localize proteins into subcellular compartments using antibodies and confocal microscopy. (genes2cognition.org)
  • led to the current notion that production of specific inositol lipids in well-defined membrane compartments upon stimulation helps recruit signaling molecules to membranes and hence contributes to the organization of signaling complexes. (biologists.org)
  • Transfer of proteins across membranes. (deepdyve.com)
  • Transfer to proteins across membranes. (deepdyve.com)
  • The complex mediates the recruitment of clathrin to the vesicle membrane. (ox.ac.uk)
  • If you cannot find the target and/or product is not available in our catalog, please click here to contact us and request the product or submit your request for custom elisa kit production , custom recombinant protein production or custom antibody production . (mybiosource.com)
  • Inositol lipids have emerged as universal lipid regulators of protein signaling complexes in defined membrane compartments. (biologists.org)
  • We investigate the molecular mechanisms by which transmembrane proteins are sorted to different compartments of the endomembrane system such as endosomes, lysosomes and a group of cell-type-specific organelles known as lysosome-related organelles (e.g., melanosomes and platelet dense bodies). (grantome.com)
  • Oppositely oriented signals that are encoded by oppositely oriented Fj and Ds gradients produce the same polarity outcome in different tissues or compartments, and the tissue-specific activity of alternative Pk-Sple protein isoforms has been observed to rectify the interpretation of opposite upstream directional signals. (stanford.edu)
  • The unfolding activity of AAA+ proteins can, however, also be deleterious to cells, in particular if linked to protein degradation, and therefore needs to be tightly controlled. (elifesciences.org)
  • A candidate gene of the bovine leukaemia virus (BLV) receptor (BLVR) was clonedpreviously and predicted to encode a transmembrane protein. (embl.de)
  • There are 6 mammalian ARFs which are regulated by over 30 guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs). (wikipedia.org)