Adaptor Protein Complex delta Subunits: A family of large adaptin protein subunits of approximately 130-kDa in size. They have been primarily found as components of ADAPTOR PROTEIN COMPLEX 3.Adaptor Protein Complex 3: An adaptor protein complex found primarily on perinuclear compartments.Adaptor Protein Complex 1: A clathrin adaptor protein complex primarily involved in clathrin-related transport at the TRANS-GOLGI NETWORK.Adaptor Protein Complex 2: An adaptor protein complex primarily involved in the formation of clathrin-related endocytotic vesicles (ENDOSOMES) at the CELL MEMBRANE.Adaptor Protein Complex 4: An adaptor protein complex involved in transport of molecules between the TRANS-GOLGI NETWORK and the endosomal-lysosomal system.Adaptor Protein Complex Subunits: The subunits that make up the large, medium and small chains of adaptor proteins.Adaptor Protein Complex mu Subunits: A family of medium adaptin protein subunits of approximately 45 KDa in size. They have been primarily found as components of ADAPTOR PROTEIN COMPLEX 3 and ADAPTOR PROTEIN COMPLEX 4.Adaptor Proteins, Vesicular Transport: A class of proteins involved in the transport of molecules via TRANSPORT VESICLES. They perform functions such as binding to the cell membrane, capturing cargo molecules and promoting the assembly of CLATHRIN. The majority of adaptor proteins exist as multi-subunit complexes, however monomeric varieties have also been found.Adaptor Protein Complex gamma Subunits: A family of large adaptin protein subunits of approximately 90 KDa in size. They have been primarily found as components of ADAPTOR PROTEIN COMPLEX 1.Adaptor Protein Complex beta Subunits: A family of large adaptin protein complex subunits of approximately 90-130 kDa in size.Adaptor Protein Complex alpha Subunits: A family of large adaptin protein subunits of approximately 100 kDa in size. They have been primarily found as components of ADAPTOR PROTEIN COMPLEX 2.
(1/20) The structure and function of the beta 2-adaptin appendage domain.

The heterotetrameric AP2 adaptor (alpha, beta 2, mu 2 and sigma 2 subunits) plays a central role in clathrin-mediated endocytosis. We present the protein recruitment function and 1.7 A resolution structure of its beta 2-appendage domain to complement those previously determined for the mu 2 subunit and alpha appendage. Using structure-directed mutagenesis, we demonstrate the ability of the beta 2 appendage alone to bind directly to clathrin and the accessory proteins AP180, epsin and eps15 at the same site. Clathrin polymerization is promoted by binding of clathrin simultaneously to the beta 2-appendage site and to a second site on the adjacent beta 2 hinge. This results in the displacement of the other ligands from the beta 2 appendage. Thus clathrin binding to an AP2-accessory protein complex would cause the controlled release of accessory proteins at sites of vesicle formation.  (+info)

(2/20) The highly conserved C-terminal dileucine motif in the cytosolic domain of the human immunodeficiency virus type 1 envelope glycoprotein is critical for its association with the AP-1 clathrin adaptor [correction of adapter].

Short amino acid sequences in the cytosolic domains of transmembrane proteins are recognized by specialized adaptor [corrected] proteins which are part of coated vesicles utilized to transport membrane proteins between the trans-Golgi network (TGN) and the plasma membrane (forward and backward). Previously, we and others reported that the membrane-proximal tyrosine residues Y712 (human immunodeficiency virus [HIV]) and Y721 (simian immunodeficiency virus [SIV]) in the envelope glycoprotein (Env) of the primate lentiviruses are crucial for the association of Env with clathrin-associated adaptor [corrected] complex AP-2. The same tyrosine-based endocytosis motifs in the cytosolic domains (EnvCD) of transmembrane gp41 of HIV type 1 (HIV-1) and SIV, respectively, were also shown to modulate the interaction with TGN- and endosome-based clathrin-associated complex AP-1. Our findings suggested that EnvCD binding to AP-1, unlike the association of EnvCD with AP-2, is dependent largely on residues other than Y712 and Y721. Here, we tested if motifs downstream of Y712 affect HIV-1 EnvCD-AP-1 binding and Env trafficking. Mutational analysis revealed that the C-terminal leucine-based motif in Env was crucial for the recruitment of AP-1 in vitro and in Env-expressing cells. In addition to affecting Env-AP-1 association, mutations at the C terminus of Env also altered the subcellular localization of Env, suggesting that proper post-Golgi routing of Env depends on its recruitment of AP-1. Finally, the C-terminal dileucine was shown to assist the membrane-proximal Y712 motif in restricting the cell surface expression of Env.  (+info)

(3/20) PACS-1 binding to adaptors is required for acidic cluster motif-mediated protein traffic.

PACS-1 is a cytosolic protein involved in controlling the correct subcellular localization of integral membrane proteins that contain acidic cluster sorting motifs, such as furin and human immunodeficiency virus type 1 (HIV-1) NEF: We have now investigated the interaction of PACS-1 with heterotetrameric adaptor complexes. PACS-1 associates with both AP-1 and AP-3, but not AP-2, and forms a ternary complex between furin and AP-1. A short sequence within PACS-1 that is essential for binding to AP-1 has been identified. Mutation of this motif yielded a dominant-negative PACS-1 molecule that can still bind to acidic cluster motifs on cargo proteins but not to adaptor complexes. Expression of dominant-negative PACS-1 causes a mislocalization of both furin and mannose 6-phosphate receptor from the trans-Golgi network, but has no effect on the localization of proteins that do not contain acidic cluster sorting motifs. Furthermore, expression of dominant-negative PACS-1 inhibits the ability of HIV-1 Nef to downregulate MHC-I. These studies demonstrate the requirement for PACS-1 interactions with adaptor proteins in multiple processes, including secretory granule biogenesis and HIV-1 pathogenesis.  (+info)

(4/20) The beta-appendages of the four adaptor-protein (AP) complexes: structure and binding properties, and identification of sorting nexin 9 as an accessory protein to AP-2.

Adaptor protein (AP) complexes are essential components for the formation of coated vesicles and the recognition of cargo proteins for intracellular transport. Each AP complex exposes two appendage domains with that function to bind regulatory accessory proteins in the cytosol. Secondary structure predictions, sequence alignments and CD spectroscopy were used to relate the beta-appendages of all human AP complexes to the previously published crystal structure of AP-2. The results suggested that the beta-appendages of AP-1, AP-2 and AP-3 have similar structures, consisting of two subdomains, whereas that of AP-4 lacks the inner subdomain. Pull-down and overlay assays showed partial overlap in the binding specificities of the beta-appendages of AP-1 and AP-2, whereas the corresponding domain of AP-3 displayed a unique binding pattern. That AP-4 may have a truncated, non-functional domain was indicated by its apparent inability to bind any proteins from cytosol. Of several novel beta-appendage-binding proteins detected, one that had affinity exclusively for AP-2 was identified as sorting nexin 9 (SNX9). SNX9, which contains a phox and an Src homology 3 domain, was found in large complexes and was at least partially associated with AP-2 in the cytosol. SNX9 may function to assist AP-2 in its role at the plasma membrane.  (+info)

(5/20) The delta subunit of AP-3 is required for efficient transport of VSV-G from the trans-Golgi network to the cell surface.

Vesicular stomatitis virus glycoprotein (VSV-G) is a transmembrane protein that functions as the surface coat of enveloped viral particles. We report the surprising result that VSV-G uses the tyrosine-based di-acidic motif (-YTDIE-) found in its cytoplasmic tail to recruit adaptor protein complex 3 for export from the trans-Golgi network. The same sorting code is used to recruit coat complex II to direct efficient transport from the endoplasmic reticulum to the Golgi apparatus. These results demonstrate that a single sorting sequence can interact with sequential coat machineries to direct transport through the secretory pathway. We propose that use of this compact sorting domain reflects a need for both efficient endoplasmic reticulum export and concentration of VSV-G into specialized post-trans-Golgi network secretory-lysosome type transport containers to facilitate formation of viral coats at the cell surface.  (+info)

(6/20) Subunit H of the V-ATPase binds to the medium chain of adaptor protein complex 2 and connects Nef to the endocytic machinery.

Nef is an accessory protein of human and simian immunodeficiency viruses (HIV and SIV) that is required for efficient viral infectivity and pathogenicity. It decreases the expression of CD4 on the surface of infected cells. V1H is the regulatory subunit H of the vacuolar membrane ATPase (V-ATPase). Previously, the interaction between Nef and V1H has been found to facilitate the internalization of CD4, suggesting that V1H could connect Nef to the endocytic machinery. In this study, we demonstrate that V1H binds to the C-terminal flexible loop in Nef from HIV-1 and to the medium chain (mu2) of the adaptor protein complex 2 (AP-2) in vitro and in vivo. The interaction sites of V1H and mu2 were mapped to a central region in V1H from positions 133 to 363, which contains 4 armadillo repeats, and to the N-terminal adaptin-binding domain in mu2 from positions 1 to 145. Fusing Nef to V1H reproduced the appropriate trafficking of Nef. This chimera internalized CD4 even in the absence of the C-terminal flexible loop in Nef. Finally, blocking the expression of V1H decreased the enhancement of virion infectivity by Nef. Thus, V1H can function as an adaptor for interactions between Nef and AP-2.  (+info)

(7/20) Phosphoinositide 3-kinase regulates beta2-adrenergic receptor endocytosis by AP-2 recruitment to the receptor/beta-arrestin complex.

Internalization of beta-adrenergic receptors (betaARs) occurs by the sequential binding of beta-arrestin, the clathrin adaptor AP-2, and clathrin. D-3 phosphoinositides, generated by the action of phosphoinositide 3-kinase (PI3K) may regulate the endocytic process; however, the precise molecular mechanism is unknown. Here we demonstrate that betaARKinase1 directly interacts with the PIK domain of PI3K to form a cytosolic complex. Overexpression of the PIK domain displaces endogenous PI3K from betaARK1 and prevents betaARK1-mediated translocation of PI3K to activated beta2ARs. Furthermore, disruption of the betaARK1/PI3K interaction inhibits agonist-stimulated AP-2 adaptor protein recruitment to the beta2AR and receptor endocytosis without affecting the internalization of other clathrin dependent processes such as internalization of the transferrin receptor. In contrast, AP-2 recruitment is enhanced in the presence of D-3 phospholipids, and receptor internalization is blocked in presence of the specific phosphatidylinositol-3,4,5-trisphosphate lipid phosphatase PTEN. These findings provide a molecular mechanism for the agonist-dependent recruitment of PI3K to betaARs, and support a role for the localized generation of D-3 phosphoinositides in regulating the recruitment of the receptor/cargo to clathrin-coated pits.  (+info)

(8/20) AP-3 directs the intracellular trafficking of HIV-1 Gag and plays a key role in particle assembly.

Gag proteins direct the process of retroviral particle assembly and form the major protein constituents of the viral core. The matrix region of the HIV-1 Gag polyprotein plays a critical role in the transport of Gag to the plasma membrane assembly site. Recent evidence indicates that Gag trafficking to late endosomal compartments, including multivesicular bodies, occurs prior to viral particle budding from the plasma membrane. Here we demonstrate that the matrix region of HIV-1 Gag interacts directly with the delta subunit of the AP-3 complex, and that this interaction plays an important functional role in particle assembly. Disruption of this interaction eliminated Gag trafficking to multivesicular bodies and diminished HIV particle formation. These studies illuminate an early step in retroviral particle assembly and provide evidence that the trafficking of Gag to late endosomes is part of a productive particle assembly pathway.  (+info)

*  AP3D1
AP-3 complex subunit delta-1 is a protein that in humans is encoded by the AP3D1 gene. AP3D1 is a subunit of the AP3 adaptor- ... "Entrez Gene: AP3D1 adaptor-related protein complex 3, delta 1 subunit". Martinez-Arca S, Rudge R, Vacca M, Raposo G, Camonis J ... "Interactions of HIV-1 nef with the mu subunits of adaptor protein complexes 1, 2, and 3: role of the dileucine-based sorting ... "Characterization of the adaptor-related protein complex, AP-3". The Journal of Cell Biology. 137 (4): 835-45. doi:10.1083/jcb. ...
*  AP3M1
The protein encoded by this gene is the medium subunit of AP-3, which is an adaptor-related protein complex associated with the ... AP-3 is a heterotetrameric protein complex composed of two large subunits (delta and beta3), a medium subunit (mu3), and a ... "Entrez Gene: AP3M1 adaptor-related protein complex 3, mu 1 subunit". Human AP3M1 genome location and AP3M1 gene details page in ... 2000). "Interactions of HIV-1 nef with the mu subunits of adaptor protein complexes 1, 2, and 3: role of the dileucine-based ...
*  Vesicular transport adaptor protein
... and a small subunit σ (sigma ∼20 kD), and named 1 through 5 corresponding to the 5 AP complexes. Components of COPI (cop one) a ... In AP-1 it is named γ (gamma), AP-2 has α (alpha), AP-3 has δ (delta), AP-4 has ε (epsilon) and AP-5 has ζ (zeta). The two ... Most of the adaptor proteins are heterotetramers. In the AP complexes, there are two large proteins (∼100 kD) and two smaller ... Vesicular transport adaptor proteins are proteins involved in forming complexes that function in the trafficking of molecules ...
*  Rnd1
Hanzal-Bayer M, Renault L, Roversi P, Wittinghofer A, Hillig RC (May 2002). "The complex of Arl2-GTP and PDE delta: from ... "Interaction of the Grb7 adapter protein with Rnd1, a new member of the Rho family". FEBS Letters. 467 (1): 91-6. doi:10.1016/ ... Nancy V, Callebaut I, El Marjou A, de Gunzburg J (Apr 2002). "The delta subunit of retinal rod cGMP phosphodiesterase regulates ... Hanzal-Bayer M, Renault L, Roversi P, Wittinghofer A, Hillig RC (May 2002). "The complex of Arl2-GTP and PDE delta: from ...
*  TRA (gene)
2001). "The Transmembrane Adaptor Protein Trim Regulates T Cell Receptor (Tcr) Expression and Tcr-Mediated Signaling via an ... San José E, Sahuquillo AG, Bragado R, Alarcón B (1998). "Assembly of the TCR/CD3 complex: CD3 epsilon/delta and CD3 epsilon/ ... "The implications of subunit interactions for the structure of the T cell receptor-CD3 complex". Eur. J. Immunol. 20 (2): 299- ... T-cell receptor alpha locus is a protein that in humans is encoded by the TRA gene, also known as TCRA or [email protected] It contributes ...
*  AP1G1
The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two ... Eiraku M, Hirata Y, Takeshima H, Hirano T, Kengaku M (Jul 2002). "Delta/notch-like epidermal growth factor (EGF)-related ... "Entrez Gene: AP1G1 adaptor-related protein complex 1, gamma 1 subunit". Fölsch H, Ohno H, Bonifacino JS, Mellman I (Oct 1999 ... "Similar subunit interactions contribute to assembly of clathrin adaptor complexes and COPI complex: analysis using yeast three- ...
*  C-Met
The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are ... The Notch pathway, through transcriptional activation of Delta ligand (see DLL3). MET mediates a complex program known as ... Furge KA, Zhang YW, Vande Woude GF (November 2000). "Met receptor tyrosine kinase: enhanced signaling through adapter proteins ... c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is ...
*  Calnexin
Bellovino D, Morimoto T, Tosetti F, Gaetani S (Jan 1996). "Retinol binding protein and transthyretin are secreted as a complex ... Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (Apr 1996). "A "double adaptor" method for improved shotgun library ... van Leeuwen JE, Kearse KP (Apr 1996). "Calnexin associates exclusively with individual CD3 delta and T cell antigen receptor ( ... Trombetta ES, Simons JF, Helenius A (Nov 1996). "Endoplasmic reticulum glucosidase II is composed of a catalytic subunit, ...
*  Akt/PKB signaling pathway
Ras-GTP then activates the p110 subunit of PI3K. Other adaptor molecules such as insulin receptor substrate (IRS) can also ... mechanistic target of rapamycin complex (mTORC) and DNA-dependent protein kinase (DNA-PK). The regulation of Ser473 ... Transcription factor NF-κB, activated by Akt, regulates peroxisome proliferator-activated receptor delta (PPARβ/δ) agonists and ... It then recruits the catalytic subunit p110 to form the fully active PI3K enzyme. Alternatively, adaptor molecule Grb2 binds to ...
*  Sec61 alpha 1
... either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also ... 2001). "Newly synthesized human delta opioid receptors retained in the endoplasmic reticulum are retrotranslocated to the ... Protein transport protein Sec61 subunit alpha isoform 1 is a protein that in humans is encoded by the SEC61A1 gene. The protein ... 2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi: ...
*  PIK3CG
"The SH2-containing adapter protein GRB10 interacts with BCR-ABL". Oncogene. 17 (8): 941-8. doi:10.1038/sj.onc.1202024. PMID ... "p210BCR/ABL induces formation of complexes containing focal adhesion proteins and the protooncogene product p120c-Cbl". Exp. ... Rommel C, Camps M, Ji H (2007). "PI3K delta and PI3K gamma: partners in crime in inflammation in rheumatoid arthritis and ... Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform is an enzyme that in humans is encoded by the ...
*  ARL3
"The delta subunit of rod specific cyclic GMP phosphodiesterase, PDE delta, interacts with the Arf-like protein Arl3 in a GTP ... Hanzal-Bayer M, Renault L, Roversi P, Wittinghofer A, Hillig RC (2002). "The complex of Arl2-GTP and PDE delta: from structure ... Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (1996). "A "double adaptor" method for improved shotgun library ... ADP-ribosylation factor-like protein 3 is a protein that in humans is encoded by the ARL3 gene. ADP-ribosylation factor-like 3 ...
*  EIF2B2
Translation initiation factor eIF-2B subunit beta is a protein that in humans is encoded by the EIF2B2 gene. Eukaryotic ... "Characterization of the mammalian initiation factor eIF2B complex as a GDP dissociation stimulator protein". J. Biol. Chem. 276 ... Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (1996). "A "double adaptor" method for improved shotgun library ... It consists of alpha (EIF2B1; MIM 606686), beta (EIF2B2), gamma (EIF2B3; MIM 606273), delta (EIF2B4; MIM 606687), and epsilon ( ...
*  List of MeSH codes (D12.776.543)
... adaptor protein complex beta subunits MeSH D12.776.543.990.150.500.300 -- adaptor protein complex delta subunits MeSH D12.776. ... adaptor protein complex gamma subunits MeSH D12.776.543.990.150.500.500 -- adaptor protein complex mu subunits MeSH D12.776. ... adaptor protein complex subunits MeSH D12.776.543.990.150.500.100 -- adaptor protein complex alpha subunits MeSH D12.776. ... adaptor protein complex 1 MeSH D12.776.543.990.150.200 -- adaptor protein complex 2 MeSH D12.776.543.990.150.300 -- adaptor ...
*  T-cell receptor
Ionizable residues in the transmembrane domain of each subunit form a polar network of interactions that hold the complex ... to bind to the ITAM and activated ZAP-70 phosphorylates tyrosines on the adaptor protein LAT, which then attracts PLC-γ. Other ... The intersection of these specific regions (V and J for the alpha or gamma chain; V, D, and J for the beta or delta chain) ... The TCR receptor complex is an octomeric complex of variable TCR receptor α and β chains with three dimeric signaling modules ...
*  WHSC2
This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in ... WHSC2 encodes the NELF-A subunit of the NELF complex. WHSC2 has been shown to interact with RDBP. GRCh38: Ensembl release 89: ... Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (Apr 1996). "A "double adaptor" method for improved shotgun library ... "Stimulation of RNA polymerase II elongation by hepatitis delta antigen". Science. 293 (5527): 124-7. doi:10.1126/science. ...
*  Lewis C. Cantley
... and that the SH2 domain of p85 specifically recognized phosphotyrosines on growth factor receptors or adaptor proteins via the ... Specifically, they discovered that the catalytic subunit p110 dimerizes with the regulatory subunit p85, ... control mTOR signaling by acting as a GTPase-activating protein complex toward Rheb". Curr. Biol. 13 (15): 1259-68. doi:10.1016 ... The first drug targeting the PI-3-kinase pathway as a treatment for cancer - Idelalisib (PI3K Delta inhibitor) - was approved ...
*  Insulin receptor
These changes facilitate the recruitment of specific adapter proteins such as the insulin receptor substrate proteins (IRS) in ... "Differential effects of tumor necrosis factor-alpha on protein kinase C isoforms alpha and delta mediate inhibition of insulin ... Downstream post-translational events of either isoform result in the formation of a proteolytically cleaved α and β subunit, ... Strictly speaking the relationship between IR and ligand shows complex allosteric properties. This was indicated with the use ...
*  Chromosome 9 (human)
G protein-coupled receptor 107 GRHPR: glyoxylate redasductase/hydroxypyruvate reductase HAUS6: HAUS augmin-like complex subunit ... delta-, dehydratase ALS4: amyotrophic lateral sclerosis 4 ANGPTL2: angiopoietin-related protein 2 ASS: argininosuccinate ... signaling threshold regulating transmembrane adapter 1 SLC25A25-AS1: encoding protein SLC25A25 antisense RNA 1 SPAG8 sperm- ... encoding protein Transmembrane protein 215 TMEM268: Transmembrane protein 268 TOR2A encoding protein Torsin-2A TSC1: tuberous ...
*  INPP5D
Leitges M, Gimborn K, Elis W, Kalesnikoff J, Hughes MR, Krystal G, Huber M (June 2002). "Protein kinase C-delta is a negative ... Poe JC, Fujimoto M, Jansen PJ, Miller AS, Tedder TF (June 2000). "CD22 forms a quaternary complex with SHIP, Grb2, and Shc. A ... Zhang S, Broxmeyer HE (January 1999). "p85 subunit of PI3 kinase does not bind to human Flt3 receptor, but associates with SHP2 ... Lemay S, Davidson D, Latour S, Veillette A (April 2000). "Dok-3, a novel adapter molecule involved in the negative regulation ...
*  Inflammasome
... the pyrin domain of the adaptor protein ASC has recently been shown to function as a prion-like domain, through a self- ... Caspase-1 then assembles into its active form consisting of two heterodimers with a p20 and p10 subunit each. Once active, it ... This NLRP1 multi-molecular complex was dubbed the 'inflammasome', which spurred much interest in the following years; since ... "The NLRP3 inflammasome modulates sleep and NREM sleep delta power induced by spontaneous wakefulness, sleep deprivation and ...
*  Chromosome 3 (human)
... encoding protein 5-azacytidine-induced protein 2 BRK1: SCAR/WAVE actin nucleating complex subunit BRPF1: bromodomain and PHD ... alpha 2/delta subunit 3 CCR5: chemokine (C-C motif) receptor 5 CGGBP1: CGG triplet repeat binding protein 1 CMTM7: CKLF like ... T-cell receptor-associated transmembrane adapter 1 USH3A: Usher syndrome 3A ZBED2: encoding protein Zinc finger BED-type ... also known as Glutamine-rich protein 1, RBM6: RNA-binding protein 6 RPP14: Ribonuclease P protein subunit p14 SCN5A: sodium ...
*  Cav1.2
The calcium channel consists of a complex of alpha-1, alpha-2/delta and beta subunits in a 1:1:1 ratio. The S3-S4 linkers of ... Calcium channel, voltage-dependent, L type, alpha 1C subunit (also known as Cav1.2) is a protein that in humans is encoded by ... Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (Apr 1996). "A "double adaptor" method for improved shotgun library ... Cav1.2 is a subunit of L-type voltage-dependent calcium channel. This gene encodes an alpha-1 subunit of a voltage-dependent ...
*  Histone acetyltransferase
Some of the proteins that associate with HATs in these complexes function by targeting the HAT complex to nucleosomes at ... E1A adenovirus protein, and S-HDAg (hepatitis delta virus small delta antigen). p300/CBP have also been observed to acetylate β ... They usually function within a multisubunit complex in which the other subunits are necessary for them to modify histone ... These complexes include SAGA (Spt/Ada/Gcn5L acetyltransferase), PCAF, ADA (transcriptional adaptor), TFIID (transcription ...
*  FYN
"Expression and characterization of the p85 subunit of the phosphatidylinositol 3-kinase complex and a related p85 beta protein ... a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in ... "Dual regulation of neuronal morphogenesis by a delta-catenin-cortactin complex and Rho". J. Cell Biol. 162 (1): 99-111. doi: ... The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. ...
Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) ELISA Kits  Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) ELISA Kits
Adaptor-Related Protein Complex 3, beta 2 Subunit ELISA Kits * Adaptor-Related Protein Complex 3, delta 1 Subunit ELISA Kits ... adapter-related protein complex 2 mu subunit , adaptin-mu2 , adaptor protein complex AP-2 subunit mu , clathrin adaptor complex ... adaptor related protein complex 2 mu 1 subunit (AP2M1) Elisa Kit * adaptor-related protein complex 2, mu 1 subunit, a (ap2m1a) ... adaptor-related protein complex 2, mu 1 subunit (Ap2m1) Elisa Kit * adaptor related protein complex 2 mu 1 subunit L homeolog ( ...
more infohttp://www.antikoerper-online.de/egfr-signaling-pathway-pathway-26/ap2m1-elisa-kit-1886/
SMART: BLVR domain annotation  SMART: BLVR domain annotation
Evaluation of the delta subunit of bovine adaptor protein complex 3 as a receptorfor bovine leukaemia virus. ... to a gene family of the delta subunit of the adaptor protein (AP)complex 3, AP-3. Therefore, bovine cDNAs (boAP3delta) that are ... Click on the protein counts, or double click on taxonomic names to display all proteins containing BLVR domain in the selected ... Complex between Vamp7 longin domain and fragment of delta-adaptin from AP3. ...
more infohttp://smart.embl.de/smart/do_annotation.pl?DOMAIN=BLVR&BLAST=DUMMY
HIV Interactions with Host Cell Proteins, Book by Paul Spearman (Paperback) | chapters.indigo.ca  HIV Interactions with Host Cell Proteins, Book by Paul Spearman (Paperback) | chapters.indigo.ca
Buy the Paperback Book HIV Interactions with Host Cell Proteins by Paul Spearman at Indigo.ca, Canada's largest bookstore. + ... The authors have recently described an interaction between Gag and the delta subunit of the AP-3 adaptor protein complex (1). ... Title:HIV Interactions with Host Cell ProteinsFormat:PaperbackDimensions:214 pagesPublished:March 1, 2012Publisher:Springer ... HIV Interactions with Host Cell Proteins. EditorPaul Spearman. Paperback , March 1, 2012. ...
more infohttps://www.chapters.indigo.ca/en-ca/books/hiv-interactions-with-host-cell/9783642261824-item.html
AP3D1 Antibody 16454-1-AP  | Proteintech  AP3D1 Antibody 16454-1-AP | Proteintech
AP3D1 fusion protein Ag9390 Full name: adaptor-related protein complex 3, delta 1 subunit ... ADTD, AP 3 complex subunit delta, AP 3 complex subunit delta 1, AP3D1, Delta adaptin, hBLVR ... Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and ... AP3D1 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin ( ...
more infohttps://www.ptglab.com/products/AP3D1-Antibody-16454-1-AP.htm
AP3D1 - Wikipedia  AP3D1 - Wikipedia
AP-3 complex subunit delta-1 is a protein that in humans is encoded by the AP3D1 gene. AP3D1 is a subunit of the AP3 adaptor- ... "Entrez Gene: AP3D1 adaptor-related protein complex 3, delta 1 subunit". Martinez-Arca S, Rudge R, Vacca M, Raposo G, Camonis J ... "Interactions of HIV-1 nef with the mu subunits of adaptor protein complexes 1, 2, and 3: role of the dileucine-based sorting ... "Characterization of the adaptor-related protein complex, AP-3". The Journal of Cell Biology. 137 (4): 835-45. doi:10.1083/jcb. ...
more infohttps://en.wikipedia.org/wiki/AP3D1
Microglia lacking a peroxisomal β-oxidation enzyme chronically alter their inflammatory profile without evoking neuronal and...  Microglia lacking a peroxisomal β-oxidation enzyme chronically alter their inflammatory profile without evoking neuronal and...
... delta 1 subunit (Ap3d1), F-box protein 38 (Fbxo38), and MON2 homolog (Mon2) were used that remain unaltered after neuronal ... ratio to the housekeeping gene β-actin except for the facial nerve injury experiment for which adaptor-related protein complex ... Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 2001; ... Clinical and biochemical spectrum of D-bifunctional protein deficiency. Ann Neurol. 2006;59(1):92-104.CrossRefGoogle Scholar ...
more infohttps://link.springer.com/article/10.1186%2Fs12974-019-1442-3
RCSB PDB - Protein Feature View 









 - AP-3 complex subunit delta-1 - O14617 (AP3D1 HUMAN)  RCSB PDB - Protein Feature View - AP-3 complex subunit delta-1 - O14617 (AP3D1 HUMAN)
... and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon ... The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, ... Subunit Structure. AP-3 associates with the BLOC-1 complex (By similarity). Adaptor protein complex 3 (AP-3) is a ... Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi ...
more infohttp://www.rcsb.org/pdb/protein/Q9H3C6
AP3抗体|Abcam中国|Anti-AP3抗体(ab3705)  AP3抗体|Abcam中国|Anti-AP3抗体(ab3705)
Adapter-related protein complex 3 subunit delta-1 antibody. *adaptor-related protein complex 3, delta 1 subunit antibody ... Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi ... Belongs to the adaptor complexes large subunit family.. Contains 11 HEAT repeats. ... The Universal Protein Resource (UniProt) in 2010. Nucleic Acids Res. 38:D142-D148 (2010) . ...
more infohttp://www.abcam.cn/ap3-antibody-ab3705.html
Ap3b2 - AP-3 complex subunit beta-2 - Mus musculus (Mouse) - Ap3b2 gene & protein  Ap3b2 - AP-3 complex subunit beta-2 - Mus musculus (Mouse) - Ap3b2 gene & protein
... and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi ... The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the ... In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into ... AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related ...
more infohttp://www.uniprot.org/uniprot/Q9JME5
AP3B1 - AP-3 complex subunit beta-1 - Canis lupus familiaris (Dog) - AP3B1 gene & protein  AP3B1 - AP-3 complex subunit beta-1 - Canis lupus familiaris (Dog) - AP3B1 gene & protein
... and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi ... The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the ... In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into ... AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related ...
more infohttp://www.uniprot.org/uniprot/Q7YRF1
Shutting down the HIV assembly line  Shutting down the HIV assembly line
... the delta subunit of AP-3, for further evaluation. AP-3 is an "adaptor protein complex," a group of four proteins known to sort ... Through a series of experiments, the group demonstrated that the AP-3 delta subunit interacts with Gag to direct it to the ... Spearman s team has focused on a protein called "Gag," the major HIV structural protein. In recent years, Spearman said, it has ... In some cell types, Gag and the HIV viral envelope protein form particles in the multivesicular body; in other cell types, Gag ...
more infohttps://www.innovations-report.com/html/reports/medicine-health/report-41637.html
P47B | definition of P47B by Medical dictionary  P47B | definition of P47B by Medical dictionary
... an adaptor-related protein complex composed of two large subunits (delta and beta), a medium subunit (mu), and a small subunit ... A gene on chromosome 8p11.2 that encodes a medium subunit of the heterotetrameric AP3 complex, ... sigma). AP3 plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes and in trafficking to ...
more infohttps://medical-dictionary.thefreedictionary.com/P47B
Clathrin adaptor complex AP3 mu-3A subunit | definition of clathrin adaptor complex AP3 mu-3A subunit by Medical dictionary  Clathrin adaptor complex AP3 mu-3A subunit | definition of clathrin adaptor complex AP3 mu-3A subunit by Medical dictionary
What is clathrin adaptor complex AP3 mu-3A subunit? Meaning of clathrin adaptor complex AP3 mu-3A subunit medical term. What ... clathrin adaptor complex AP3 mu-3A subunit explanation free. ... does clathrin adaptor complex AP3 mu-3A subunit mean? ... Looking for online definition of clathrin adaptor complex AP3 mu-3A subunit in the Medical Dictionary? ... an adaptor-related protein complex composed of two large subunits (delta and beta), a medium subunit (mu), and a small subunit ...
more infohttp://medical-dictionary.thefreedictionary.com/clathrin+adaptor+complex+AP3+mu-3A+subunit
Comparison of Proteins Secreted into Extracellular Space of Pathogenic and Non-pathogenic <i xmlns=>Acanthamoeba...  Comparison of Proteins Secreted into Extracellular Space of Pathogenic and Non-pathogenic <i xmlns="">Acanthamoeba...
hypothetical protein ACA1_052470 [Acanthamoeba castellanii Neff]. 54. 38.7. IN26. adaptor protein complex AP-3 delta subunit [ ... 7 qualitative increased proteins, 65 decreased proteins, and 17 qualitative decreased proteins in ACP strain. Twenty protein ... where 34 proteins increased (IN), 7 proteins qualitatively increased (QI), 65 proteins decreased (DE), and 17 proteins ... Proteins highly expressed in pathogenic A. castellanii Among 41 up-regulated protein spots, 13 proteins with more than 5-fold ...
more infohttp://parasitol.kr/journal/view.php?viewtype=pubreader&number=2265
In silico and in vitro analysis of boAP3d1 protein interaction with bovine leukaemia virus gp51<...  In silico and in vitro analysis of boAP3d1 protein interaction with bovine leukaemia virus gp51<...
Although the bovine adaptor protein 3 complex subunit delta-1 (boAP3D1) has been proposed as the potential receptor, the ... Although the bovine adaptor protein 3 complex subunit delta-1 (boAP3D1) has been proposed as the potential receptor, the ... Although the bovine adaptor protein 3 complex subunit delta-1 (boAP3D1) has been proposed as the potential receptor, the ... Although the bovine adaptor protein 3 complex subunit delta-1 (boAP3D1) has been proposed as the potential receptor, the ...
more infohttps://pure.urosario.edu.co/en/publications/in-silico-and-in-vitro-analysis-of-boap3d1-protein-interaction-wi
Recombinant Human AP2S1 293 Cell Lysate AP2S1-8811HCL - Creative BioMart  Recombinant Human AP2S1 293 Cell Lysate AP2S1-8811HCL - Creative BioMart
... adapter-related protein complex 2 sigma subunit; clathrin-associated/assembly/adaptor protein, small 2 (17kD); AP17; AP17-DELTA ... adaptor-related protein complex 2, sigma 1 subunit; CLAPS2; AP-2 complex subunit sigma; sigma2-adaptin; HA2 17 kDa subunit; ... clathrin assembly protein 2 small chain; adaptor protein complex AP-2 subunit sigma; plasma membrane adaptor AP-2 17 kDa ... Products > Cancer Proteins > Signal Transduction Proteins > Protein Trafficking > Golgi Proteins Golgi Proteins MAPK3 PLIN2 ...
more infohttps://www.creativebiomart.net/description_410458_318.htm
AP2S1 rabbit monoclonal antibody - (H00001175-K) - Products - Abnova  AP2S1 rabbit monoclonal antibody - (H00001175-K) - Products - Abnova
AP17,AP17-DELTA,CLAPS2. *Gene Description:. *adaptor-related protein complex 2, sigma 1 subunit ... clathrin assembly protein 2 small chain,clathrin-associated/assembly/adaptor protein, small 2 (17kD) ... One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane ... This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this ...
more infohttp://www.abnova.com/products/products_detail.asp?catalog_id=H00001175-K
AP3M1 Gene - GeneCards | AP3M1 Protein | AP3M1 Antibody  AP3M1 Gene - GeneCards | AP3M1 Protein | AP3M1 Antibody
Protein Coding), Adaptor Related Protein Complex 3 Mu 1 Subunit, including: function, proteins, disorders, pathways, orthologs ... Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type ... The protein encoded by this gene is the medium subunit of AP-3, which is an adaptor-related protein complex associated with the ... AP3M1 (Adaptor Related Protein Complex 3 Mu 1 Subunit) is a Protein Coding gene. Among its related pathways are Chaperonin- ...
more infohttp://www.genecards.org/cgi-bin/carddisp.pl?gene=AP3M1
AP3M1 - Wikipedia  AP3M1 - Wikipedia
The protein encoded by this gene is the medium subunit of AP-3, which is an adaptor-related protein complex associated with the ... AP-3 is a heterotetrameric protein complex composed of two large subunits (delta and beta3), a medium subunit (mu3), and a ... "Entrez Gene: AP3M1 adaptor-related protein complex 3, mu 1 subunit". Human AP3M1 genome location and AP3M1 gene details page in ... 2000). "Interactions of HIV-1 nef with the mu subunits of adaptor protein complexes 1, 2, and 3: role of the dileucine-based ...
more infohttps://en.wikipedia.org/wiki/AP3M1
Notch Signaling | www.antibodies-online.com  Notch Signaling | www.antibodies-online.com
This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP ... DLL3 (delta Like Protein 3): DLL3 antibodies DLL3 ELISA Kits DLL3 Proteins ... DLL4 (delta-Like 4): DLL4 antibodies DLL4 ELISA Kits DLL4 Proteins JAG2 (Jagged 2): JAG2 antibodies JAG2 ELISA Kits JAG2 ... FBXW7 Proteins NEDD4 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase): NEDD4 ...
more infohttps://www.antibodies-online.com/notch-signaling-pathway-14/
  • p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors. (uniprot.org)
  • Mutations in one of the large subunits of AP-3 have been associated with the Hermansky-Pudlak syndrome, a genetic disorder characterized by defective lysosome-related organelles. (genecards.org)
  • also studied ClpC proteins that had mutations to the middle domain that prevented the repressed state from forming. (elifesciences.org)
  • Among its related pathways are Chaperonin-mediated protein folding and Metabolism of proteins . (genecards.org)
  • As in other multicellular organisms, RAS proteins play a key role in the signaling pathways that regulate cell growth and differentiation in Drosophila . (springer.com)
  • They are key players in protein quality control by targeting misfolded and aggregated proteins to degrading and refolding pathways. (elifesciences.org)
  • Through a series of experiments, the group demonstrated that the AP-3 delta subunit interacts with Gag to direct it to the multivesicular body. (innovations-report.com)
  • AP-1A recruitment to membranes can be modulated by Phosphofurin Acidic Cluster Sorting protein 1 (PACS-1), a cytosolic protein which interacts with both AP-1A and cargo that has been phosphorylated by casein kinase II. (frontiersin.org)
  • We are calling this complex AP-3, a name that has also been used for the neuronalspecific phosphoprotein AP180, but we feel that it is amore appropriate designation for an adaptor-related heterotetramer.Immunofluorescence using anti-delta antibodies reveals that the AP-3 complex isassociated with the Golgi region of the cell as well as with more peripheralstructures. (embl.de)
  • Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest. (uniprot.org)
  • Thedelta subunit is closely related to the protein product of the Drosophila garnet gene, which when mutated results in reduced pigmentation of the eyes and othertissues. (embl.de)
  • Although these proteins were first identified in Drosophila using cross-species hybridization, it has been the use of classical genetics which has overwhelmingly driven investigations of RAS function in Drosophila . (springer.com)
  • Localization of the sevenless protein, a putative receptor for positional information, in the eye imaginal disc of Drosophila . (springer.com)
  • The authors have recently described an interaction between Gag and the delta subunit of the AP-3 adaptor protein complex (1). (indigo.ca)
  • Disruption of the interaction, using a specific piece of the AP-3 subunit, eliminated Gag trafficking to the multivesicular body and diminished HIV particle formation in cells. (innovations-report.com)
  • Although the bovine adaptor protein 3 complex subunit delta-1 (boAP3D1) has been proposed as the potential receptor, the specific ligand-receptor interaction has not yet been completely defined and boAP3D1 receptor and gp51 3D structures have not been determined. (urosario.edu.co)
  • The boAP3D1 receptor interaction patterns were calculated based on models of boAP3D1 receptor and gp51 complexes' 3D structures, which were constructed using homology techniques and data-driven docking strategy. (urosario.edu.co)
  • For soluble proteins, luminal pH and divalent metals can affect aggregation and interaction with surrounding membranes. (frontiersin.org)
  • We identify dynamin and the EAP -binding alpha-adaptin appendage domain of the AP2 adaptor as switches in a regulated, multistep maturation process and provide direct evidence for a molecular checkpoint in clathrin mediated endocytosis. (antikoerper-online.de)
  • AAA+ ( A TPase a ssociated with a variety of cellular a ctivities) proteins control a multitude of essential cellular activities by converting the energy derived from ATP hydrolysis into a mechanical force to remodel bound substrates ( Hanson and Whiteheart, 2005 ). (elifesciences.org)
  • To gain more information on virulence factors of Acanthamoeba infection, the objective of this study was to compare the levels of proteins secreted into extracellular space between pathogenic ATCC 30011 strain and non-pathogenic ATCC 30868 strain of Acanthamoeba . (parasitol.kr)
  • Expression levels of proteins secreted into extracellular space were compared between A. castellanii pathogenic (ACP) and non-pathogenic strains. (parasitol.kr)
  • Differentially expressed proteins were identified, focusing on highly expressed proteins in pathogenic Acanthamoeba . (parasitol.kr)
  • have now used a technique called cryo-electron microscopy to study the structures of repressed and adapter-activated ClpC from pathogenic bacteria called Staphylococcus aureus . (elifesciences.org)
  • This complex is composed of two large chains, a medium chain, and a small chain. (abnova.com)
  • Basler K, Hafen E. Control of photoreceptor cell fate by the sevenless protein requires a functional tyrosine kinase domain. (springer.com)
  • Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. (antibodies-online.com)
  • Subsequent cloning ofrelated genes from other organisms indicated that the candidate gene is related, but unique, to a gene family of the delta subunit of the adaptor protein (AP)complex 3, AP-3. (embl.de)
  • PCGF3: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. (mybiosource.com)
  • Their stability as reference genes was validated by NormFinder, BestKeeper, geNorm and Delta Ct programs. (biomedcentral.com)
  • AP-3 associates with the BLOC-1 complex (By similarity). (rcsb.org)
  • These proteins showed similarity each to inosine-uridine preferring nucleoside hydrolase, carboxylesterase, oxygen-dependent choline dehydrogenase, periplasmic-binding protein proteinases and hypothetical proteins. (parasitol.kr)
  • During contact with human vaginal epithelial cells, Trichomonas vaginalis releases 19 major proteins, including cysteine proteases [ 10 ]. (parasitol.kr)
  • ATP-dependent AAA+ proteases form ring-shaped assemblies that are composed of AAA+ proteins and an associated peptidase. (elifesciences.org)
  • Alternatively spliced transcript variants encoding the same protein have been observed. (wikipedia.org)
  • A boAP3delta protein taggedwith green fluorescent protein was localized in the cytoplasm and incorporatedinto AP-3 in bovine cells. (embl.de)