ADAM Proteins: A family of membrane-anchored glycoproteins that contain a disintegrin and metalloprotease domain. They are responsible for the proteolytic cleavage of many transmembrane proteins and the release of their extracellular domain.Antigens, CD40: A member of the tumor necrosis factor receptor superfamily with specificity for CD40 LIGAND. It is found on mature B-LYMPHOCYTES and some EPITHELIAL CELLS, lymphoid DENDRITIC CELLS. Evidence suggests that CD40-dependent activation of B-cells is important for generation of memory B-cells within the germinal centers. Mutations of the gene for CD40 antigen result in HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 3. Signaling of the receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.Bronchoconstriction: Narrowing of the caliber of the BRONCHI, physiologically or as a result of pharmacological intervention.Immunoglobulin Class Switching: Gene rearrangement of the B-lymphocyte which results in a substitution in the type of heavy-chain constant region that is expressed. This allows the effector response to change while the antigen binding specificity (variable region) remains the same. The majority of class switching occurs by a DNA recombination event but it also can take place at the level of RNA processing.Lymphocyte Activation: Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.B-Lymphocytes: Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.Receptors, Antigen, T-Cell: Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.CD40 Ligand: A membrane glycoprotein and differentiation antigen expressed on the surface of T-cells that binds to CD40 ANTIGENS on B-LYMPHOCYTES and induces their proliferation. Mutation of the gene for CD40 ligand is a cause of HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 1.Immunoglobulin Switch Region: A site located in the INTRONS at the 5' end of each constant region segment of a immunoglobulin heavy-chain gene where recombination (or rearrangement) occur during IMMUNOGLOBULIN CLASS SWITCHING. Ig switch regions are found on genes encoding all five classes (IMMUNOGLOBULIN ISOTYPES) of IMMUNOGLOBULIN HEAVY CHAINS.Plants, Genetically Modified: PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.Diet: Regular course of eating and drinking adopted by a person or animal.Plant Leaves: Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)Plant Proteins: Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.Plant Roots: The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)Genes, Plant: The functional hereditary units of PLANTS.Plant Extracts: Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard.Plant Shoots: New immature growth of a plant including stem, leaves, tips of branches, and SEEDLINGS.Plants, Medicinal: Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals.Fitness Centers: Facilities having programs intended to promote and maintain a state of physical well-being for optimal performance and health.Public Relations: Relations of an individual, association, organization, hospital, or corporation with the publics which it must take into consideration in carrying out its functions. Publics may include consumers, patients, pressure groups, departments, etc.Glutens: Prolamins in the endosperm of SEEDS from the Triticeae tribe which includes species of WHEAT; BARLEY; and RYE.LondonSoybeans: An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS.Blogging: Using an INTERNET based personal journal which may consist of reflections, comments, and often hyperlinks.Dietary Proteins: Proteins obtained from foods. They are the main source of the ESSENTIAL AMINO ACIDS.Soy Foods: Foods made from SOYBEANS. Health benefits are ascribed to the high levels of DIETARY PROTEINS and ISOFLAVONES.Aging: The gradual irreversible changes in structure and function of an organism that occur as a result of the passage of time.Mitochondrial Degradation: Proteolytic breakdown of the MITOCHONDRIA.Mitochondrial Proteins: Proteins encoded by the mitochondrial genome or proteins encoded by the nuclear genome that are imported to and resident in the MITOCHONDRIA.Quality Control: A system for verifying and maintaining a desired level of quality in a product or process by careful planning, use of proper equipment, continued inspection, and corrective action as required. (Random House Unabridged Dictionary, 2d ed)Mitochondria: Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)KuwaitMolecular Chaperones: A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. Although they take part in the assembly process, molecular chaperones are not components of the final structures.Antiphospholipid Syndrome: The presence of antibodies directed against phospholipids (ANTIBODIES, ANTIPHOSPHOLIPID). The condition is associated with a variety of diseases, notably systemic lupus erythematosus and other connective tissue diseases, thrombopenia, and arterial or venous thromboses. In pregnancy it can cause abortion. Of the phospholipids, the cardiolipins show markedly elevated levels of anticardiolipin antibodies (ANTIBODIES, ANTICARDIOLIPIN). Present also are high levels of lupus anticoagulant (LUPUS COAGULATION INHIBITOR).Alzheimer Disease: A degenerative disease of the BRAIN characterized by the insidious onset of DEMENTIA. Impairment of MEMORY, judgment, attention span, and problem solving skills are followed by severe APRAXIAS and a global loss of cognitive abilities. The condition primarily occurs after age 60, and is marked pathologically by severe cortical atrophy and the triad of SENILE PLAQUES; NEUROFIBRILLARY TANGLES; and NEUROPIL THREADS. (From Adams et al., Principles of Neurology, 6th ed, pp1049-57)Cell Adhesion: Adherence of cells to surfaces or to other cells.Hyperhomocysteinemia: Condition in which the plasma levels of homocysteine and related metabolites are elevated (>13.9 µmol/l). Hyperhomocysteinemia can be familial or acquired. Development of the acquired hyperhomocysteinemia is mostly associated with vitamins B and/or folate deficiency (e.g., PERNICIOUS ANEMIA, vitamin malabsorption). Familial hyperhomocysteinemia often results in a more severe elevation of total homocysteine and excretion into the urine, resulting in HOMOCYSTINURIA. Hyperhomocysteinemia is a risk factor for cardiovascular and neurodegenerative diseases, osteoporotic fractures and complications during pregnancy.Monocytes: Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.Vascular Cell Adhesion Molecule-1: Cytokine-induced cell adhesion molecule present on activated endothelial cells, tissue macrophages, dendritic cells, bone marrow fibroblasts, myoblasts, and myotubes. It is important for the recruitment of leukocytes to sites of inflammation. (From Pigott & Power, The Adhesion Molecule FactsBook, 1993, p154)Intercellular Adhesion Molecule-1: A cell-surface ligand involved in leukocyte adhesion and inflammation. Its production is induced by gamma-interferon and it is required for neutrophil migration into inflamed tissue.Endothelium, Vascular: Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.Coculture Techniques: A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.Cell Communication: Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.Endothelial Cells: Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.Glial Fibrillary Acidic Protein: An intermediate filament protein found only in glial cells or cells of glial origin. MW 51,000.DNA Nucleotidylexotransferase: A non-template-directed DNA polymerase normally found in vertebrate thymus and bone marrow. It catalyzes the elongation of oligo- or polydeoxynucleotide chains and is widely used as a tool in the differential diagnosis of acute leukemias in man. EC 2.7.7.31.Search Engine: Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.Brain Injuries: Acute and chronic (see also BRAIN INJURIES, CHRONIC) injuries to the brain, including the cerebral hemispheres, CEREBELLUM, and BRAIN STEM. Clinical manifestations depend on the nature of injury. Diffuse trauma to the brain is frequently associated with DIFFUSE AXONAL INJURY or COMA, POST-TRAUMATIC. Localized injuries may be associated with NEUROBEHAVIORAL MANIFESTATIONS; HEMIPARESIS, or other focal neurologic deficits.Astrocytoma: Neoplasms of the brain and spinal cord derived from glial cells which vary from histologically benign forms to highly anaplastic and malignant tumors. Fibrillary astrocytomas are the most common type and may be classified in order of increasing malignancy (grades I through IV). In the first two decades of life, astrocytomas tend to originate in the cerebellar hemispheres; in adults, they most frequently arise in the cerebrum and frequently undergo malignant transformation. (From Devita et al., Cancer: Principles and Practice of Oncology, 5th ed, pp2013-7; Holland et al., Cancer Medicine, 3d ed, p1082)In Situ Nick-End Labeling: An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.Astrocytes: A class of large neuroglial (macroglial) cells in the central nervous system - the largest and most numerous neuroglial cells in the brain and spinal cord. Astrocytes (from "star" cells) are irregularly shaped with many long processes, including those with "end feet" which form the glial (limiting) membrane and directly and indirectly contribute to the BLOOD-BRAIN BARRIER. They regulate the extracellular ionic and chemical environment, and "reactive astrocytes" (along with MICROGLIA) respond to injury.Microglia: The third type of glial cell, along with astrocytes and oligodendrocytes (which together form the macroglia). Microglia vary in appearance depending on developmental stage, functional state, and anatomical location; subtype terms include ramified, perivascular, ameboid, resting, and activated. Microglia clearly are capable of phagocytosis and play an important role in a wide spectrum of neuropathologies. They have also been suggested to act in several other roles including in secretion (e.g., of cytokines and neural growth factors), in immunological processing (e.g., antigen presentation), and in central nervous system development and remodeling.Spinal Cord: A cylindrical column of tissue that lies within the vertebral canal. It is composed of WHITE MATTER and GRAY MATTER.Tumor Necrosis Factor-alpha: Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.Fatigue: The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Systems Integration: The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Proteome: The protein complement of an organism coded for by its genome.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Phosphines: Inorganic or organic compounds derived from phosphine (PH3) by the replacement of H atoms. (From Grant & Hackh's Chemical Dictionary, 5th ed)User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Sodium Azide: A cytochrome oxidase inhibitor which is a nitridizing agent and an inhibitor of terminal oxidation. (From Merck Index, 12th ed)Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Antibodies, Viral: Immunoglobulins produced in response to VIRAL ANTIGENS.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Azides: Organic or inorganic compounds that contain the -N3 group.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Antibody Formation: The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.Antibodies, Neutralizing: Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.
(1/1953) Constitutive and regulated alpha-secretase cleavage of Alzheimer's amyloid precursor protein by a disintegrin metalloprotease.

Amyloid beta peptide (Abeta), the principal proteinaceous component of amyloid plaques in brains of Alzheimer's disease patients, is derived by proteolytic cleavage of the amyloid precursor protein (APP). Proteolytic cleavage of APP by a putative alpha-secretase within the Abeta sequence precludes the formation of the amyloidogenic peptides and leads to the release of soluble APPsalpha into the medium. By overexpression of a disintegrin and metalloprotease (ADAM), classified as ADAM 10, in HEK 293 cells, basal and protein kinase C-stimulated alpha-secretase activity was increased severalfold. The proteolytically activated form of ADAM 10 was localized by cell surface biotinylation in the plasma membrane, but the majority of the proenzyme was found in the Golgi. These results support the view that APP is cleaved both at the cell surface and along the secretory pathway. Endogenous alpha-secretase activity was inhibited by a dominant negative form of ADAM 10 with a point mutation in the zinc binding site. Studies with purified ADAM 10 and Abeta fragments confirm the correct alpha-secretase cleavage site and demonstrate a dependence on the substrate's conformation. Our results provide evidence that ADAM 10 has alpha-secretase activity and many properties expected for the proteolytic processing of APP. Increases of its expression and activity might be beneficial for the treatment of Alzheimer's disease.  (+info)

(2/1953) Unaltered cleavage and secretion of angiotensin-converting enzyme in tumor necrosis factor-alpha-converting enzyme-deficient mice.

Mammalian angiotensin-converting enzyme (ACE) is one of several biologically important ectoproteins that exist in both membrane-bound and soluble forms as a result of a post-translational proteolytic cleavage. It has been suggested that a common proteolytic system is responsible for the cleavage of a diverse group of membrane ectoproteins, and tumor necrosis factor-alpha-converting enzyme (TACE), a recently purified disintegrin-metalloprotease, has been implicated in the proteolytic cleavage of several cell surface proteins. Mice devoid of TACE have been developed by gene targeting. Such mice could provide a useful system to determine if TACE is responsible for the cleavage of other ectoproteins. Cultured fibroblasts without TACE activity, when transfected with cDNA encoding for the testicular isozyme of ACE (ACET), synthesized and secreted ACET normally after a proteolytic cleavage near the C terminus. In addition, similar quantities of the soluble, C-terminally truncated somatic isozyme of ACE (ACEP) were present in the serum of wild-type and TACE-deficient mice. These results demonstrate that TACE is not essential in the generation of soluble ACE under physiological conditions. Finally, we also report solubilization of ACE-secretase, the enzyme that cleaves ACE, from mouse ACE89 cells and from rabbit lung. We demonstrate that soluble ACE-secretase from both sources failed to cleave its substrate in solution, suggesting a requirement for anchoring to the membrane.  (+info)

(3/1953) Thrombotic thrombocytopenic purpura and autoimmunity: a tale of shadows and suspects.

BACKGROUND AND OBJECTIVE: The key pathogenic feature of TTP is the formation of platelet aggregates within the microcirculation; however, the etiology of such aggregates has been elusive for years. A large amount of evidence points to an abnormal interaction between damaged vascular endothelium and platelets, although the cause of the primary microvascular endothelial cell injury is seldom clear. The autoimmune hypothesis often recurs, and this is based on a number of observations: the claimed superiority of plasma-exchange over plasma infusion, the anecdotal report of the presence of immunocomplexes and autoantibodies in TTP patients, the efficacy of the administration of corticosteroids and other immunosuppressant agents, and the concomitant occurrence of TTP in association with autoimmune diseases, especially systemic lupus erythematosus (SLE). This review will focus on the complex relationships between TTP and humoral autoimmunity; in particular, similarities and differences between TTP, SLE and antiphospholipid (aPL) antibodies syndrome, as well as the putative role of several other antibodies directed towards endothelial cells and/or platelets, including the recently discovered anti-CD36 antibodies and antivWF-cleaving metalloprotease, will be discussed. DESIGN AND METHODS: The authors have been involved in the study and treatment of TTP and autoimmune diseases for years; furthermore, the PubMed data base of the National Library of Congress has been extensively searched using the Internet. CONCLUSIONS: Although over the years evidence has increased in favor of the autoimmune hypothesis for TTP etiopathogenesis, TTP should not yet be considered an autoimmune disease. Autoantibodies should be regarded as only one of the many different insults which can trigger microvascular thrombosis even though the autoimmune theory of the pathogenesis of TTP is gaining more and more strength. As far as concerns the relationship between TTP, SLE and aPL antibodies-related disorders, these diseases should be distinguished on the basis of both different clinical presentations and accurate antibody screening, although this approach should definitely not delay the prompt start of treatment.  (+info)

(4/1953) Regulation of human ADAM 12 protease by the prodomain. Evidence for a functional cysteine switch.

The ADAMs (a disintegrin and metalloprotease) are a family of multidomain proteins that are believed to play key roles in cell-cell and cell-matrix interactions. We have shown recently that human ADAM 12-S (meltrin alpha) is an active metalloprotease. It is synthesized as a zymogen, with the prodomain maintaining the protease in a latent form. We now provide evidence that the latency mechanism of ADAM 12 can be explained by the cysteine switch model, in which coordination of Zn2+ in the active site of the catalytic domain by a cysteine residue in the prodomain is critical for inhibition of the protease. Replacing Cys179 with other amino acids results in an ADAM 12 proform that is proteolytically active, but latency can be restored by placing cysteine at other positions in the propeptide. None of the amino acids adjacent to the crucial cysteine residue is essential for blocking activity of the protease domain. In addition to its latency function, the prodomain is required for exit of ADAM 12 protease from the endoplasmic reticulum. Tissue inhibitor of metalloprotease-1, -2, and -3 were not found to block proteolytic activity of ADAM 12, hence a physiological inhibitor of ADAM 12 protease in the extracellular environment remains to be identified.  (+info)

(5/1953) Evidence for a role of a tumor necrosis factor-alpha (TNF-alpha)-converting enzyme-like protease in shedding of TRANCE, a TNF family member involved in osteoclastogenesis and dendritic cell survival.

Tumor necrosis factor (TNF)-related activation-induced cytokine (TRANCE), a member of the TNF family, is a dendritic cell survival factor and is essential for osteoclastogenesis and osteoclast activation. In this report we demonstrate (i) that TRANCE, like TNF-alpha, is made as a membrane-anchored precursor, which is released from the plasma membrane by a metalloprotease; (ii) that soluble TRANCE has potent dendritic cell survival and osteoclastogenic activity; (iii) that the metalloprotease-disintegrin TNF-alpha convertase (TACE) can cleave immunoprecipitated TRANCE in vitro in a fashion that mimics the cleavage observed in tissue culture cells; and (iv) that in vitro cleavage of a TRANCE ectodomain/CD8 fusion protein and of a peptide corresponding to the TRANCE cleavage site by TACE occurs at the same site that is used when TRANCE is shed from cells into the supernatant. We propose that the TRANCE ectodomain is released from cells by TACE or a related metalloprotease-disintegrin, and that this release is an important component of the function of TRANCE in bone and immune homeostasis.  (+info)

(6/1953) Deficient activity of von Willebrand's factor-cleaving protease in patients with disseminated malignancies.

An aberrant platelet immunorelated glycoprotein Ib (GPIb) receptor expressed by human tumor cells appears to participate in primary adhesive interactions required for the metastatic process. Hence, we questioned whether plasma von Willebrand's factor (vWf), its adhesive ligand, manifested comparable anomalies in patients with disseminated tumors. Plasma specimens from patients with disseminated metastases showed 68% (P < 0.013), 91% (P < 0.0009), and 207% (P < 0.0009) enhancements in FVIII:C activity, vWf-related antigen levels, and ristocetin co-factor activity, respectively, whereas their SDS-agarose electrophoretic analysis demonstrated a 165% (P < 0.001) increase in the highly polymeric forms of vWf compared to control preparations from patients with corresponding, localized solid tumors. Substantially reduced levels of vWf-cleaving protease activity were observed in study patient specimens, with no plasma inhibitors detectable. The clinical presence and absence of tumor metastases correlated significantly with vWf-cleaving enzyme activities of < or = 15% and > or = 88%, respectively (n = 20; P < 0.0001). Finally, with an in vitro model system, tumor-induced platelet aggregation was enhanced by 127% (P < 0.001) in study patient platelet-rich plasma (PRP) compared to control PRP and could be completely inhibited (P < 0.0009) when both tumor cells and their PRP substrates were incubated with monoclonal antibodies directed against the vWf binding epitope of GPIb alpha and against the GPIb binding epitope of plasma vWf, respectively. Unusually large vWf multimers observed in patients with disseminated tumors probably result from deficient vWf-cleaving protease activity and may represent a novel mechanism regulating primary platelet-tumor adhesive interactions involved in the metastatic process.  (+info)

(7/1953) Cysteine-rich domain of human ADAM 12 (meltrin alpha) supports tumor cell adhesion.

The ADAMs (A disintegrin and metalloprotease) comprise a family of membrane-anchored cell surface proteins with a putative role in cell-cell and/or cell-matrix interactions. By immunostaining, ADAM 12 (meltrin alpha) was up-regulated in several human carcinomas and could be detected along the tumor cell membranes. Because of this intriguing staining pattern, we investigated whether human ADAM 12 supports tumor cell adhesion. Using an in vitro assay using recombinant polypeptides expressed in Escherichia coli, we examined the ability of individual domains of human ADAM 12 and ADAM 15 to support tumor cell adhesion. We found that the disintegrin-like domain of human ADAM 15 supported adhesion of alphavbeta3-expressing A375 melanoma cells. In the case of human ADAM 12, however, recombinant polypeptides of the cysteine-rich domain but not the disintegrin-like domain supported cell adhesion of a panel of carcinoma cell lines. On attachment to recombinant polypeptides from the cysteine-rich domain of human ADAM 12, most tumor cell lines, such as MDA-MB-231 breast carcinoma cells, were rounded and associated with numerous actin-containing filopodia and used a cell surface heparan sulfate proteoglycan to attach. Finally, we demonstrated that authentic full-length human ADAM 12 could bind to heparin Sepharose. Together these results suggest a novel role of the cysteine-rich domain of ADAM 12 -- that of supporting tumor cell adhesion.  (+info)

(8/1953) Evidence that a functional fertilin-like ADAM plays a role in human sperm-oolemmal interactions.

Fertilin is a protein initially identified in guinea pig spermatozoa; it is the prototype of a larger family of conserved, proteins designated as a disintegrin and a metalloproteinase (ADAM). These heterodimers which consist of alpha and beta subunits, containing metalloproteinase-like and disintegrin-like domains, appear to play a role in mammalian fertilization. Peptides derived from the disintegrin domains of two ADAMs, fertilin and cyritestin, interfere with gamete adhesion and sperm-egg membrane fusion in non-human species. It has been suggested that fertilin-beta binds to an oolemmal integrin, and it is proposed that the tripeptide FEE (Phe-Glu-Glu) is the integrin recognition sequence in human fertilin-beta. We evaluated whether fertilin beta plays a role in human fertilization by studying the effects of a linear octapeptide containing the FEE sequence, SFEECDLP, and a scrambled octapeptide with the same amino acids, SFPCEDEL, on the incorporation of human spermatozoa by human zona-free eggs. The effects of G4120, a potent RGD-containing (Arg-Gly-Asp) thioether-bridged cyclic peptide which blocks both fibronectin and vitronectin receptors, and the relationship between FEE- and RGD-receptor interactions on sperm-egg interactions were also studied. The FEE-containing peptide, but not the scrampled peptide, inhibited sperm adhesion to oocytes and their penetration, over the range 1-5 microM. The inhibition induced by SFEECDLP was reversible and occurred only in the presence of peptide itself. The G4120 peptide exhibited 10-fold less inhibitory effects on sperm adhesion and penetration than did SFEECDLP. When combined, SFEECDLP and G4120 exhibited strong inhibition of both adhesion and penetration at concentrations that individually had been ineffective, suggesting co-operation between the two receptor-ligand interactions during fertilization. We propose that a fertilin-like molecule is functionally active on human spermatozoa and that its interaction with an oolemmal integrin receptor plays a role in fertilization in humans.  (+info)

*  ADAM (protein)
ADAM22 ADAM metallopeptidase domain 22". "Entrez Gene: ADAM23 ADAM metallopeptidase domain 23". "Entrez Gene: ADAM28 ADAM ... ADAM metallopeptidase domain 7". "Entrez Gene: ADAM8 ADAM metallopeptidase domain 8". "Entrez Gene: ADAM9 ADAM metallopeptidase ... 1995). "ADAM, a widely distributed and developmentally regulated gene family encoding membrane proteins with a disintegrin and ... ADAMTS (A disintegrin and metalloproteinase with thrombospondin motifs) family Ectodomain shedding ADAM Proteins at the US ...
*  Metalloproteinase
Well known metalloendopeptidases include ADAM proteins and matrix metalloproteinases. In the MEROPS database peptidase families ... The metal ion is coordinated to the protein via three ligands. The ligands co-ordinating the metal ion can vary with histidine ... In many instances the structural protein fold that characterises the clan or family may have lost its catalytic activity, yet ... Minde DP, Maurice MM, Rüdiger SG (2012). "Determining biophysical protein stability in lysates by a fast proteolysis assay, ...
*  ADAM28
The protein encoded by this gene is a lymphocyte-expressed ADAM protein. Alternative splicing results in two transcript ... This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane- ... Disintegrin and metalloproteinase domain-containing protein 28 is an enzyme that in humans is encoded by the ADAM28 gene. ... 2002). "The lymphocyte metalloprotease MDC-L (ADAM 28) is a ligand for the integrin alpha4beta1". J. Biol. Chem. 277 (5): 3784- ...
*  Disintegrin
ADAM Protein Evolution of snake venom McLane MA, Sanchez EE, Wong A, Paquette-Straub C, Perez JC (2004). "Disintegrins". Curr ... ADAM and ADAMTS protein families, which include important protease enzymes. As an example, the secreted protease ADAMTS13, ... Disintegrin-like proteins are found in various species ranging from slime mold to humans. Some other proteins known to contain ... The beta-subunit of guinea pig sperm surface protein PH30. PH30 is a protein involved in sperm-egg fusion. The beta subunit ...
*  Alpha secretase
Other ADAM proteins, ADAM17 (also called TACE, tumor necrosis factor-α converting enzyme), ADAM9, and ADAM19 have also been ... have also been implicated in similar maturation events for other transmembrane proteins such as MHC class I proteins. Recent ... "Protein kinase C-dependent alpha-secretase competes with beta-secretase for cleavage of amyloid-beta precursor protein in the ... Several such proteins, notably ADAM10, have been identified as possessing alpha-secretase activity. Upon cleavage by alpha ...
*  ADAM8
"ADAM family proteins in the immune system". Immunology Today. 20 (6): 278-84. doi:10.1016/S0167-5699(99)01464-4. PMID 10354553 ... "Entrez Gene: ADAM8 ADAM metallopeptidase domain 8". Yamamoto S, Higuchi Y, Yoshiyama K, Shimizu E, Kataoka M, Hijiya N, ... The protein encoded by this gene may be involved in cell adhesion during neurodegeneration. Cluster of differentiation GRCh38: ... A Disintegrin and metalloproteinase domain-containing protein 8 is an enzyme that in humans is encoded by the ADAM8 gene. This ...
*  ADAM10
Cluster of differentiation ADAM 17 Metallopeptidase ADAM Protein GRCh38: Ensembl release 89: ENSG00000137845 - Ensembl, May ... Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease ... Wolfsberg TG, Primakoff P, Myles DG, White JM (1995). "ADAM, a novel family of membrane proteins containing A Disintegrin And ... Chubinskaya S, Mikhail R, Deutsch A, Tindal MH (2001). "ADAM-10 protein is present in human articular cartilage primarily in ...
*  List of MeSH codes (D09)
... adam proteins MeSH D09.408.051.059 --- anthracyclines MeSH D09.408.051.059.050 --- aclarubicin MeSH D09.408.051.059.200 --- ...
*  ADAM17
... is a 70-kDa enzyme that belongs to the ADAM protein family of disintegrins and metalloproteases. ADAM17 is an 824-amino acid ... Hirohata S, Seldin MF, Apte SS (November 1998). "Chromosomal assignment of two ADAM genes, TACE (ADAM17) and MLTNB (ADAM19), to ... ADAM metallopeptidase domain 17 (ADAM17), also called TACE (tumor necrosis factor-α-converting enzyme), ... Kärkkäinen I, Rybnikova E, Pelto-Huikko M, Huovila AP (June 2000). "Metalloprotease-disintegrin (ADAM) genes are widely and ...
*  List of MeSH codes (D08)
... adam proteins MeSH D08.811.277.656.675.374.205 --- collagenases MeSH D08.811.277.656.675.374.205.352 --- gelatinase a MeSH ... proto-oncogene proteins c-mdm2 MeSH D08.811.464.938.750.750 --- skp cullin f-box protein ligases MeSH D08.811.464.938.750.750. ... gtp-binding protein alpha subunit, gi2 MeSH D08.811.277.040.330.300.200.100.300 --- gtp-binding protein alpha subunits, gq-g11 ... proto-oncogene proteins c-bcr MeSH D08.811.913.696.620.682.700.776 --- proto-oncogene proteins c-pim-1 MeSH D08.811.913.696. ...
*  Immunocontraception
... that have been identified as potential targets for immunocontraception include the sperm-specific peptides or proteins ADAM, ... Wang, M; JL Shi; GY Cheng; YQ Hu; C Xu (2009). "The antibody against a nuclear autoantigenic sperm protein can result in ... Inoue, N; M Ikawa; A Isotani; M Okabe (2005). "The immunoglobin superfamily protein izumo is required for sperm to fuse with ... Because the antigens used in contraceptive vaccines are protein, not steroids, they are not easily passed from animal to animal ...
*  ADAM15
The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family ... Primakoff P, Myles DG (2000). "The ADAM gene family: surface proteins with adhesion and protease activity". Trends Genet. 16 (2 ... It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein ... 2003). "The adaptor protein fish associates with members of the ADAMs family and localizes to podosomes of Src-transformed ...
*  ADAM9
2003). "Expression of ADAM-9 mRNA and protein in human breast cancer". Int. J. Cancer. 105 (6): 754-61. doi:10.1002/ijc.11161. ... The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, ... 2003). "ADAM-9 is an insulin-like growth factor binding protein-5 protease produced and secreted by human osteoblasts". ... "Entrez Gene: ADAM9 ADAM metallopeptidase domain 9 (meltrin gamma)". Nelson KK, Schlöndorff J, Blobel CP (Nov 1999). "Evidence ...
*  Sheddase
Many sheddases are members of the ADAM or aspartic protease (BACE) protein families. These enzymes can activate a transmembrane ... protein if it is a receptor (e.g., HER2), or cut off the part of the transmembrane protein which has already bound an agonist ( ... Tumor necrosis factor alpha converting enzyme (TACE) is a sheddase protein that has been observed in many types of cancer and ... Sheddases are membrane-bound enzymes that cleave extracellular portions of transmembrane proteins, releasing the soluble ...
*  ADAM12
ADAM 12, a metalloprotease that binds insulin growth factor binding protein-3 (IGFBP-3), appears to be an effective early Down ... This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane- ... Decreased levels of ADAM 12 may be detected in cases of trisomy 21 as early as 8 to 10 weeks gestation. Maternal serum ADAM 12 ... Loechel F, Fox JW, Murphy G, Albrechtsen R, Wewer UM (Nov 2000). "ADAM 12-S cleaves IGFBP-3 and IGFBP-5 and is inhibited by ...
*  ADAM11
This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane- ... "Entrez Gene: ADAM11 ADAM metallopeptidase domain 11". Wolfsberg TG, Primakoff P, Myles DG, White JM (1995). "ADAM, a novel ... Disintegrin and metalloproteinase domain-containing protein 11 is an enzyme that in humans is encoded by the ADAM11 gene. ... Sagane K, Ohya Y, Hasegawa Y, Tanaka I (1998). "Metalloproteinase-like, disintegrin-like, cysteine-rich proteins MDC2 and MDC3 ...
*  Proteases in angiogenesis
"Structure-function analysis of the ADAM family of diintegrin-like and metalloproteinase-containing proteins". J. Protein Chem. ... The ADAM family of proteases is receiving increased attention for their ability to alter the balance between pro-and anti- ... Native proteins which contain such domains with regulatory activity are normally inactive, most likely because they are cryptic ... Most disintegrins contain this conserved RGD motif, but ADAM15 is the only member of the ADAM family to contain this motif. A ...
*  ADAMDEC1
ADAM-like, decysin 1 is a protein that in humans is encoded by the ADAMDEC1 gene. This encoded protein is thought to be a ... This protein may play an important role in dendritic cell function and their interactions with germinal center T cells. [ ... Fritsche, J; Müller, A; Hausmann, M; Rogler, G; Andreesen, R; Kreutz, M (2003). "Inverse regulation of the ADAM-family members ... provided by RefSeq, Jul 2008]. "Entrez Gene: ADAM-like, decysin 1". Retrieved 2014-08-16. Mueller, C. G.; Rissoan, M. C.; ...
*  ADAM7
... is an 85-kDa enzyme that is a member of the transmembrane ADAM (A Disintegrin and Metalloprotease) protein family. ... ADAM7 is also denoted as: ADAM_7, ADAM-7, EAPI, GP-83, and GP83. The functions of ADAM7 directly relate to sperm maturation and ... Disintegrin and metalloproteinase domain-containing protein 7 is a protein that in humans is encoded by the ADAM7 gene. ... Lacking protease activity, ADAM7 may play roles in protein-protein interactions and cell adhesion processes including sperm-egg ...
*  Aggrecan
... is a proteoglycan, or a protein modified with large carbohydrates; the human form of the protein is 2316 amino acids ... Proteases capable of degrading aggrecans are called aggrecanases, and they are members of the ADAM (A Disintegrin And ... chains are attached to an extended protein core. Aggrecan has a molecular mass >2,500 kDa. The core protein (~300 kDa) has ... The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in ...
*  ADAM2
"Role of the integrin-associated protein CD9 in binding between sperm ADAM 2 and the egg integrin alpha6beta1: implications for ... immunolocalization and processing of fertilins ADAM-1 and ADAM-2 in the boar (Sus domesticus) spermatozoa during epididymal ... "Entrez Gene: ADAM2 ADAM metallopeptidase domain 2 (fertilin beta)". Eto K, Huet C, Tarui T, Kupriyanov S, Liu HZ, Puzon- ... This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane- ...
*  Lamin
Intermediate filament Nuclear lamina Laminopathies Inner nuclear membrane proteins Dechat, Thomas; Adam, Stephen A.; Taimen, ... This is accomplished by lamin and lamin-interacting proteins (SUN1/SUN2) connecting with proteins on the outer nuclear membrane ... protein family. Further investigations found evidence that supports that all IF proteins arose from a common lamin-like ... Lamin proteins are involved in the disassembling and reforming of the nuclear envelope during mitosis, the positioning of ...
*  Chromosome 5 (human)
ABLIM3: encoding protein Actin-binding LIM protein 3 ADAMTS2: ADAM metallopeptidase with thrombospondin type 1 motif, 2 AGXT2: ... encoding protein FAM114A2 C5orf13: Neuronal regeneration related protein C5orf21/FAM172A: encoding protein UPF0528 protein ... Protein Red IRX1: Iroquois-class homeodomain protein (human) LARP1: La-related protein 1 LMAN2: Lectin mannose binding 2 LNCR3 ... encoding protein PWWP domain containing 2A RANBP3L: encoding protein RAN binding protein 3-like RMND5B: Required for meiotic ...
*  Chromosome 9 (human)
ABO: ABO histo-blood group glycosyltransferases ACTL7A: encoding protein Actin-like protein 7A ADAMTS13: ADAM metallopeptidase ... encoding protein Transmembrane protein 215 TMEM268: Transmembrane protein 268 TOR2A encoding protein Torsin-2A TSC1: tuberous ... protein prune homolog 2 RABGAP1: RAB GTPase activating protein 1 REXO4: RNA exonuclease 4 RNF183: encoding protein Ring finger ... zinc finger protein basonuclin-2 C9orf64: chromosome 9 open reading frame 64 C9orf78: encoding protein Uncharacterized protein ...
*  Green iguana
Zoologists, such as Adam Britton, believe that such a diet containing protein is unhealthy for the animal's digestive system ... Britton, Adam (2007-04-19). "Animal Protein and Claw Trimming". Melissa Kaplan's Herp Care Collection. Retrieved 2007-06-27. " ... There is some debate as to whether captive green iguanas should be fed animal protein. There is evidence of wild iguanas eating ... Although green iguanas in captivity will eat meat if presented with it, excessive consumption of animal protein results in ...
*  Fragmentation (cell biology)
Genes involved in controlling cell death encode proteins with three distinct functions: "Killer" proteins are required for a ... Smith, Aaron; Parkes, Michael AF; Atkin-Smith, Georgia K; Tixeira, Rochelle; Poon, Ivan KH. "Cell disassembly during apoptosis ... cell to begin the apoptotic process "Destruction" proteins do things such as digest DNA in a dying cell "Engulfment" proteins ... CAD is usually inhibited by another protein in the cell, called Inhibitor of caspase-activated DNase (ICAD). In order for ...
Cleavage of CD23 by ADAM proteins is regulated by beta-2 adrenergic receptor engagement on primed B cells (55.11) | The Journal...  Cleavage of CD23 by ADAM proteins is regulated by beta-2 adrenergic receptor engagement on primed B cells (55.11) | The Journal...
Cleavage of CD23 by ADAM proteins is regulated by beta-2 adrenergic receptor engagement on primed B cells (55.11). Caroline ... Cleavage of CD23 by ADAM proteins is regulated by beta-2 adrenergic receptor engagement on primed B cells (55.11) ... Cleavage of CD23 by ADAM proteins is regulated by beta-2 adrenergic receptor engagement on primed B cells (55.11) ... Cleavage of CD23 by ADAM proteins is regulated by beta-2 adrenergic receptor engagement on primed B cells (55.11) ...
more infohttp://www.jimmunol.org/content/186/1_Supplement/55.11
ADAM (protein) - Wikipedia  ADAM (protein) - Wikipedia
ADAM22 ADAM metallopeptidase domain 22". "Entrez Gene: ADAM23 ADAM metallopeptidase domain 23". "Entrez Gene: ADAM28 ADAM ... ADAM metallopeptidase domain 7". "Entrez Gene: ADAM8 ADAM metallopeptidase domain 8". "Entrez Gene: ADAM9 ADAM metallopeptidase ... 1995). "ADAM, a widely distributed and developmentally regulated gene family encoding membrane proteins with a disintegrin and ... ADAMTS (A disintegrin and metalloproteinase with thrombospondin motifs) family Ectodomain shedding ADAM Proteins at the US ...
more infohttps://en.wikipedia.org/wiki/ADAM_(protein)
Post Exercise Ingestion of Plant vs Animal Protein by Adam N. Upshaw  "Post Exercise Ingestion of Plant vs Animal Protein" by Adam N. Upshaw
Study 2 demonstrated that regardless of protein type (hemp, dairy milk or whey protein isolate) post training session CHO- ... Six weeks of concurrent training, when supplemented with hemp based protein-CHO drink post exercise, appears to be effective at ... Thus, post training session CHO-protein ingestion appears to be as effective for strength development over 10 weeks of training ... However, debate exists about whether the type of protein consumed, animal vs plant, affects exercise performance differently. ...
more infohttps://ir.lib.uwo.ca/etd/2271/
Protein  Protein
Chocolate Covered Almond Protein Balls…HELL YEAH!!. by Adam Stansbury , July 18, 2017 , Nutrition, Protein, Recipe, Vegan ... Creating Protein Packed Plant Powered Recipes. by Adam Stansbury , September 22, 2017 , Education, Nutrition, Protein, Vegan ... Sources of Plant Based Proteins. by Adam Stansbury , September 22, 2017 , Education, From Meat to Plants, Nutrition, Protein, ... The Protein Myth. by Adam Stansbury , August 18, 2017 , Education, Nutrition, Protein, Vegan ...
more infohttp://www.theplantpoweredpt.com/category/protein/
Crystal structures of catrocollastatin/VAP2B reveal a dynamic, modular architecture of ADAM/adamalysin/reprolysin family...  Crystal structures of catrocollastatin/VAP2B reveal a dynamic, modular architecture of ADAM/adamalysin/reprolysin family...
... modular architecture of ADAM/adamalysin/reprolysin family proteins.. Igarashi T1, Araki S, Mori H, Takeda S. ... ADAM)/adamalysin/reprolysin family proteins. Here we describe crystal structures of catrocollastatin/VAP2B in three different ... modular architecture that may be important for the functions of ADAM/adamalysin/reprolysin family proteins. ... Catrocollastatin/vascular apoptosis-inducing protein (VAP)2B is a metalloproteinase from Crotalus atrox venom, possessing ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/17485084?dopt=Abstract
Evidence against roles for phorbol binding protein Munc13-1, ADAM adap by Annat F Ikin, Mirsada Causevic et al.  "Evidence against roles for phorbol binding protein Munc13-1, ADAM adap" by Annat F Ikin, Mirsada Causevic et al.
Eve-1, an ADAM adaptor protein reported to be essential for PKC-regulated shedding of pro-EGF, was found to play no obvious ... compounds that act via protein kinase C (PKC) or through unconventional phorbol-binding proteins such as Munc13-1. We have ... Here, we examined the effects on APP ectodomain shedding of four phorbol-sensitive proteins involved in regulation of vesicular ... Shedding of the Alzheimer amyloid precursor protein (APP) ectodomain can be accelerated by phorbol esters, ...
more infohttp://jdc.jefferson.edu/farberneursofp/2/
Sources of Plant Based Proteins - Adam Stansbury - The Plant Powered Personal Trainer - Online, London, Chelsea, Fulham  Sources of Plant Based Proteins - Adam Stansbury - The Plant Powered Personal Trainer - Online, London, Chelsea, Fulham
Sources of Plant Based Proteins. by Adam Stansbury , September 22, 2017 , Education, From Meat to Plants, Nutrition, Protein, ... Plant Protein Powders. The three most widely used plant sources found in protein powders are generally hemp, pea and rice due ... Usually a good protein powder will have a blend of two or all three of them to give the powder a broad amino acid profile, ... As you could see from the lists of food groups in my last blog that most of the protein obtained on a plant based diet comes ...
more infohttp://www.theplantpoweredpt.com/sources-of-plant-based-proteins/
Role of Mitophagy in Mitochondrial Protein Quality Control - Adam Hughes  Role of Mitophagy in Mitochondrial Protein Quality Control - Adam Hughes
Hughes, Adam / University of Utah. NIH 2015. R00 AG. Role of Mitophagy in Mitochondrial Protein Quality Control. Hughes, Adam ... Role of Mitophagy in Mitochondrial Protein Quality Control Hughes, Adam University of Utah, Salt Lake City, UT, United States ... Role of Mitophagy in Mitochondrial Protein Quality Control. Hughes, Adam / University of Utah. $249,000. ... Rutter, Jared; Hughes, Adam L (2015) Power(2): the power of yeast genetics applied to the powerhouse of the cell. Trends ...
more infohttp://grantome.com/grant/NIH/R00-AG043095-02
Homocysteinylated albumin promotes increased monocyte-endothelial cell adhesion and up-regulation of MCP1, Hsp60 and ADAM17.  Homocysteinylated albumin promotes increased monocyte-endothelial cell adhesion and up-regulation of MCP1, Hsp60 and ADAM17.
... and it has been hypothesized that homocysteinylated proteins are important mediators of the toxic effects of ... The cardiovascular risk factor homocysteine is mainly bound to proteins in human plasma, ... 0/Albumins; 0/CCL2 protein, human; 0/Chaperonin 60; 0/Chemokine CCL2; 454-28-4/Homocysteine; EC 3.4.24.-/ADAM Proteins; EC 3.4. ... ADAM Proteins / metabolism*. Albumins / chemistry, physiology*. Cell Adhesion. Cell Line. Chaperonin 60 / metabolism*. ...
more infohttp://www.biomedsearch.com/nih/Homocysteinylated-Albumin-Promotes-Increased-Monocyte/22319627.html
Systematic discovery of ectopic pregnancy serum biomarkers using 3-D protein profiling coupled with label-free quantitation.  Systematic discovery of ectopic pregnancy serum biomarkers using 3-D protein profiling coupled with label-free quantitation.
A 3-D biomarker discovery strategy consisting of abundant protein immunodepletion, SDS gels, ... 0/Biological Markers; 0/Blood Proteins; 0/Membrane Proteins; 0/Proteome; EC 3.4.24.-/ADAM 12 protein; EC 3.4.24.-/ADAM Proteins ... ADAM Proteins / blood, genetics. Amino Acid Sequence. Biological Markers / blood*. Blood Proteins / analysis*. Chromatography, ... Databases, Protein. Electrophoresis, Polyacrylamide Gel / methods. Female. Humans. Membrane Proteins / blood, genetics. ...
more infohttp://www.biomedsearch.com/nih/Systematic-Discovery-Ectopic-Pregnancy-Serum/21142075.html
ITGAL | Cancer Genetics Web  ITGAL | Cancer Genetics Web
Heat shock protein 90 is critical for regulation of phenotype and functional activity of human T lymphocytes and NK cells.. J ... What pathways are this gene/protein implicaed in?. Show (14). ITGAL is involved in:. - Adhesion and Diapedesis of Granulocytes ... What does this gene/protein do?. Show (23). ITGAL is implicated in:. - activated T cell proliferation - blood coagulation - ... protein heterodimerization activity - regulation of immune response - signal transduction - T cell activation via T cell ...
more infohttp://www.cancerindex.org/geneweb/ITGAL.htm
Similar articles for PubMed (Select 20034386) - PubMed - NCBI  Similar articles for PubMed (Select 20034386) - PubMed - NCBI
ADAM and ADAMTS Family Proteins and Snake Venom Metalloproteinases: A Structural Overview. ... A Disintegrin and Metalloproteinase (ADAM) and ADAM with thrombospondin motifs (ADAMTS) family in vascular biology and disease. ... Emerging roles of ADAM and ADAMTS metalloproteinases in cancer.. Rocks N, Paulissen G, El Hour M, Quesada F, Crahay C, Gueders ... Role of ADAM and ADAMTS metalloproteinases in airway diseases.. Paulissen G, Rocks N, Gueders MM, Crahay C, Quesada-Calvo F, ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed?linkname=pubmed_pubmed&from_uid=20034386
Calories in Adams Organic French Onion Soup - Calorie, Fat, Carb, Fiber, and Protein Info | SparkRecipes  Calories in Adam's Organic French Onion Soup - Calorie, Fat, Carb, Fiber, and Protein Info | SparkRecipes
Full nutritional breakdown of the calories in Adam's Organic French Onion Soup based on the calories and nutrition in each ... Calories in Adam's Organic French Onion Soup. View the full Adam's Organic French Onion Soup Recipe & Instructions. Submitted ... Calories per serving of Adam's Organic French Onion Soup. 36 calories of Earth Balance Natural Buttery Spread, (0.36 tbsp). 29 ...
more infohttps://recipes.sparkpeople.com/recipe-calories.asp?recipe=2605627
Determination of An Interaction between Nipped B-Like Protein and MLL by Adam Robert Marek  "Determination of An Interaction between Nipped B-Like Protein and MLL" by Adam Robert Marek
The NIPBL protein is quite large; so seven subdomain regions were cloned and expressed. GST-tagged MLL repression domain was ... protein serves as a positive transcriptional regulator during hematopoietic and embryonic development. The MLL gene can undergo ... Marek, Adam Robert, "Determination of An Interaction between Nipped B-Like Protein and MLL" (2012). Master's Theses. 825. https ... The NIPBL protein is quite large; so seven subdomain regions were cloned and expressed. GST-tagged MLL repression domain was ...
more infohttps://ecommons.luc.edu/luc_theses/825/
Identification and functional analysis of endothelial tip cell-enriched genes | Blood Journal  Identification and functional analysis of endothelial tip cell-enriched genes | Blood Journal
ADAM proteins-therapeutic potential in cancer. Curr Cancer Drug Targets 2008;8(8):720-732. ... 2 are secreted proteins that stabilize the BM by interacting with other ECM proteins such as laminin, collagen IV, perlecan, ... A) Protein extracts from APLN- and APJ-deficient retinas were analyzed by Western blot for mTOR and ERK-phosphorylation. Total ... Proteases up-regulated in DLL4+/− retinas included uPAR, ADAM-TS family members, and cathepsin S, which are known to facilitate ...
more infohttp://www.bloodjournal.org/content/116/19/4025?ijkey=bed8809397085c09e8a092e06fc128bf6b43c1ac&keytype2=tf_ipsecsha&sso-checked=true
ADAMTS6 - ADAM metallopeptidase with thrombospondin type 1 motif 6 - Ornithorhynchus anatinus (Duckbill platypus) - ADAMTS6...  ADAMTS6 - ADAM metallopeptidase with thrombospondin type 1 motif 6 - Ornithorhynchus anatinus (Duckbill platypus) - ADAMTS6...
Pfam protein domain database. More...Pfami. View protein in Pfam. PF17771 ADAM_CR_2, 1 hit. PF05986 ADAM_spacer1, 1 hit. ... PROSITE; a protein domain and family database. More...PROSITEi. View protein in PROSITE. PS50215 ADAM_MEPRO, 1 hit. PS50092 ... View protein in Pfam. PF17771 ADAM_CR_2, 1 hit. PF05986 ADAM_spacer1, 1 hit. PF01562 Pep_M12B_propep, 1 hit. PF01421 ... View protein in InterPro. IPR041645 ADAM_CR_2. IPR010294 ADAM_spacer1. IPR013273 ADAMTS/ADAMTS-like. IPR024079 MetalloPept_cat_ ...
more infohttps://www.uniprot.org/uniprot/F7DL37
Decysin is highly expressed in mature DCs. (A) 28 cycle | Open-i  Decysin is highly expressed in mature DCs. (A) 28 cycle | Open-i
ADAM Proteins. *Amino Acid Sequence. *Antigens, CD11/immunology. *Base Sequence. *Blotting, Northern ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC2199019_JEM.970967f4a&req=4
The ADAMs family of proteases: new biomarkers and therapeutic targets for cancer? | Clinical Proteomics | Full Text  The ADAMs family of proteases: new biomarkers and therapeutic targets for cancer? | Clinical Proteomics | Full Text
Furthermore, a number of selective ADAM inhibitors, especially against ADAM10 and ADAM17, have been shown to have anti-cancer ... As proteases, their main substrates are the ectodomains of other transmembrane proteins. These substrates include precursor ... The ADAMs are transmembrane proteins implicated in proteolysis and cell adhesion. Forty gene members of the family have been ... Structure of ADAM Proteins. The generalised structure of an ADAM protein contains 8 distinct domains or regions. In the typical ...
more infohttps://clinicalproteomicsjournal.biomedcentral.com/articles/10.1186/1559-0275-8-9
astrocytic tumor spinal cord 2005:2010[pubdate] *count=100 - BioMedLib™ search engine  astrocytic tumor spinal cord 2005:2010[pubdate] *count=100 - BioMedLib™ search engine
ADAM Proteins; EC 3.4.24.- / Metalloendopeptidases; EC 3.4.24.86 / ADAM17 Protein; EC 3.4.24.86 / Adam17 protein, rat ... Plumb J, Cross AK, Surr J, Haddock G, Smith T, Bunning RA, Woodroofe MN: ADAM-17 and TIMP3 protein and mRNA expression in ... Title] ADAM-17 and TIMP3 protein and mRNA expression in spinal cord white matter of rats with acute experimental autoimmune ... Chemical-registry-number] 0 / Antigens; 0 / Ectodysplasins; 0 / Glial Fibrillary Acidic Protein; 0 / Membrane Proteins; 0 / RNA ...
more infohttp://www.bmlsearch.com/?kwr=astrocytic+tumor+spinal+cord+2005:2010%5Bpubdate%5D&cxts=100&stmp=b0
Differences in Extracellular Matrix Production and Basic Fibroblast Growth Factor Response in Skin Fibroblasts from Sporadic...  Differences in Extracellular Matrix Production and Basic Fibroblast Growth Factor Response in Skin Fibroblasts from Sporadic...
2000) Altered cell-matrix associated ADAM proteins in Alzheimer disease. J. Neurosc. Res. 59:680-4.CrossRefGoogle Scholar ... Scatchard G. (1949) The attractions of proteins for small molecules and ions. Ann. NY Acad. Science. 51:660-72.CrossRefGoogle ... 1997) Amyloid precursor proteins protect neurons of transgenic mice against acute and chronic excitotoxic injuries in vivo. ... Lahiri DK, Nall C. (1995) Promoter activity of the gene encoding the beta-amyloid precursor protein is up-regulated by growth ...
more infohttps://rd.springer.com/article/10.2119%2F2007-00034.Bellucci