One of the FURANS with a carbonyl thereby forming a cyclic lactone. It is an endogenous compound made from gamma-aminobutyrate and is the precursor of gamma-hydroxybutyrate. It is also used as a pharmacological agent and solvent.
S-Acyl coenzyme A. Fatty acid coenzyme A derivatives that are involved in the biosynthesis and oxidation of fatty acids as well as in ceramide formation.
The addition of an organic acid radical into a molecule.

Thermoregulation of N-acyl homoserine lactone-based quorum sensing in the soft rot bacterium Pectobacterium atrosepticum. (1/173)

The psychrotolerant bacterium Pectobacterium atrosepticum produces four N-acyl homoserine lactones under a wide range of temperatures. Their thermoregulation differs from that of the exoenzyme production, described as being under quorum-sensing control. A mechanism involved in this thermoregulation consists of controlling N-acyl homoserine lactones synthase production at a transcriptional level.  (+info)

The plant pathogen Pantoea ananatis produces N-acylhomoserine lactone and causes center rot disease of onion by quorum sensing. (2/173)

A number of gram-negative bacteria have a quorum-sensing system and produce N-acyl-l-homoserine lactone (AHL) that they use them as a quorum-sensing signal molecule. Pantoea ananatis is reported as a common colonist of wheat heads at ripening and causes center rot of onion. In this study, we demonstrated that P. ananatis SK-1 produced two AHLs, N-hexanoyl-l-homoserine lactone (C6-HSL) and N-(3-oxohexanoyl)-l-homoserine lactone (3-oxo-C6-HSL). We cloned the AHL-synthase gene (eanI) and AHL-receptor gene (eanR) and revealed that the deduced amino acid sequence of EanI/EanR showed high identity to those of EsaI/EsaR from P. stewartii. EanR repressed the ean box sequence and the addition of AHLs resulted in derepression of ean box. Inactivation of the chromosomal eanI gene in SK-1 caused disruption of exopolysaccharide (EPS) biosynthesis, biofilm formation, and infection of onion leaves, which were recovered by adding exogenous 3-oxo-C6-HSL. These results demonstrated that the quorum-sensing system involved the biosynthesis of EPS, biofilm formation, and infection of onion leaves in P. ananatis SK-1.  (+info)

Theoretical study of molecular determinants involved in signal binding to the TraR protein of Agrobacterium tumefaciens. (3/173)

N-acylated homoserine lactone (AHL) mediated cell-cell communication in bacteria is dependent on the recognition of the cognate signal by its receptor. This interaction allows the receptor-ligand complex to act as a transcriptional activator, controlling the expression of a range of bacterial phenotypes, including virulence factor expression and biofilm formation. One approach to determine the key features of signal- binding is to model the intermolecular interactions between the receptor and ligand using computational-based modeling software (LigandFit). In this communication, we have modeled the crystal structure of the AHL receptor protein TraR and its AHL signal N-(3- oxooctanoyl)-homoserine lactone from Agrobacterium tumefaciens and compared it to the previously reported antagonist behaviour of a number of AHL analogues, in an attempt to determine structural constraints for ligand binding. We conclude that (i) a common conformation of the AHL in the hydrophobic and hydrophilic region exists for ligand-binding, (ii) a tail chain length threshold of 8 carbons is most favourable for ligand-binding affinity, (iii) the positive correlation in the docking studies could be used a virtual screening tool.  (+info)

Production of acyl-homoserine lactone quorum-sensing signals is widespread in gram-negative Methylobacterium. (4/173)

Members of Methylobacterium, referred as pink-pigmented facultative methylotrophic bacteria, are frequently associated with terrestrial and aquatic plants, tending to form aggregates on the phyllosphere. We report here that the production of autoinducer molecules involved in the cell-to-cell signaling process, which is known as quorum sensing, is common among Methylobacterium species. Several strains of Methylobacterium were tested for their ability to produce N-acyl-homoserine lactone (AHL) signal molecules using different indicators. Most strains of Methylobacterium tested could elicit a positive response in Agrobacterium tumefaciens harboring lacZ fused to a gene that is regulated by autoinduction. The synthesis of these compounds was cell-density dependent, and the maximal activity was reached during the late exponential to stationary phases. The bacterial extracts were separated by thin-layer chromatography and bioassayed with A. tumefaciens NT1 (traR, tra::lacZ749). They revealed the production of various patterns of the signal molecules, which are strain dependent. At least two signal molecules could be detected in most of the strains tested, and comparison of their relative mobilities suggested that they are homologs of N-octanoyl-DL-homoserine lactone (C8-HSL) and N-decanoyl-DL-homoserine lactone (C10-HSL).  (+info)

The acyl-homoserine lactone-type quorum-sensing system modulates cell motility and virulence of Erwinia chrysanthemi pv. zeae. (5/173)

Erwinia chrysanthemi pv. zeae is one of the Erwinia chrysanthemi pathovars that infects on both dicotyledons and monocotyledons. However, little is known about the molecular basis and regulatory mechanisms of its virulence. By using a transposon mutagenesis approach, we cloned the genes coding for an E. chrysanthemi pv. zeae synthase of acyl-homoserine lactone (AHL) quorum-sensing signals (expI(Ecz)) and a cognate response regulator (expR(Ecz)). Chromatography analysis showed that expI(Ecz) encoded production of the AHL signal N-(3-oxo-hexanoyl)-homoserine lactone (OHHL). Null mutation of expI(Ecz) in the E. chrysanthemi pv. zeae strain EC1 abolished AHL production, increased bacterial swimming and swarming motility, disabled formation of multicell aggregates, and attenuated virulence of the pathogen on potato tubers. The mutation also marginally reduced the inhibitory activity of E. chrysanthemi pv. zeae on rice seed germination. The mutant phenotypes were rescued by either exogenous addition of AHL signal or in trans expression of expI(Ecz). These data demonstrate that the AHL-type QS signal plays an essential role in modulation of E. chrysanthemi pv. zeae cell motility and the ability to form multicell aggregates and is involved in regulation of bacterial virulence.  (+info)

Multiple phenotypic alterations caused by a c-type cytochrome maturation ccmC gene mutation in Pseudomonas aeruginosa. (6/173)

 (+info)

Inter-kingdom signalling: communication between bacteria and their hosts. (7/173)

 (+info)

Characteristics of the LrhA subfamily of transcriptional regulators from Sinorhizobium meliloti. (8/173)

In our previous work, we identified 94 putative genes encoding LysR-type transcriptional regulators from Sinorhizobium meliloti. All of these putative lysR genes were mutagenized using plasmid insertions to determine their phenotypes. Six LysR-type regulators, encoded by mutants SMa1979, SMb20715, SMc00820, SMc04163, SMc03975, and SMc04315, showed similar amino acid sequences (30%) and shared the conserved DNA-binding domain with LrhA, HexA, or DgdR. Phenotype analysis of these gene mutants indicated that the regulators control the swimming behaviors of the bacteria, production of quorum-sensing signals, and secretion of extracellular proteins. These characteristics are very similar to those of LrhA, HexA, and DgdR. Thus, we refer to this group as the LrhA subfamily. Sequence analysis showed that a great number of homologous genes of the LrhA subfamily were distributed in the alpha, beta, and gamma subdivisions of proteobacteria, and a few in actinobacteria. These findings could provide new clues to the roles of the LysR gene family.  (+info)

Author Summary The human pathogen Pseudomonas aeruginosa and other bacteria communicate with each other using quorum sensing (QS). This is important for their growth, virulence, motility and the formation of biofilms. Furthermore, eukaryotic cells
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Implications for the application of the MPN technique.It has been shown that cellular isolates which correspond to abundant 16S rDNA sequences can indeed be isolated if the inocula are diluted in MPN series prior to enrichment (16). This effect has been attributed to the presence of rare but rapidly growing bacteria which are eliminated by the dilution step. Consequently, the MPN technique currently represents the method of choice to obtain cultures of abundant bacteria (16).. Unexpectedly, isolates obtained in the present study from the highest positive dilutions, despite the very high cultivation success of up to 100%, could not be detected by DGGE fingerprinting and yielded only a very faint signal during dot blot hybridization. Based on the relatively large inherent statistical uncertainty of the MPN technique, the fraction of strain G100 in the natural bacterioplankton community could also be as low as 12% (the lower limit of the MPN confidence interval). Nevertheless, this minimum value is ...
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Specific recognition of cognate signals is considered fundamental to cell signaling circuits as it creates fidelity in the communication system. In bacterial quorum sensing (QS), receptor specificity ensures that bacteria cooperate only with kin. There are examples, however, of QS receptors that respond promiscuously to multiple signals. Eavesdropping by these promiscuous receptors can be beneficial in both interspecies competition... ...
Bacterial cell-to-cell signalling has emerged as a new area in microbiology. Individual bacterial cells communicate with each other and co-ordinate group activities. Although a lot of detail is known about the mechanisms of a few well-characterized bacterial communication systems, other systems have …
Virulence in the Gram-negative pathogen Pseudomonas aeruginosa relies in part on the efficient functioning of two LuxI/R dependent quorum sensing (QS) cascades, namely, the LasI/R and RhlI/R systems that generate and respond to N-(3-oxo)-dodecanoyl-l-homoserine lactone and N-butyryl-l-homoserine lactone, respectively. The two acyl homoserine lactone (AHL) synthases, LasI and RhlI, use 3-oxododecanoyl-ACP and butyryl-ACP, respectively, as the acyl-substrates to generate the corresponding autoinducer signals for the bacterium. Although AHL synthases represent excellent targets for developing QS modulators in P. aeruginosa, and in other related bacteria, the identification of potent and signal synthase specific inhibitors has represented a significant technical challenge. In the current study, we sought to test the utility of AHL analogs as potential modulators of an AHL synthase and selected RhlI in P. aeruginosa as an initial target. We systematically varied the chemical functionalities of the ...
A number of bacteria use a class of chemical compounds called acyl-homoserine lactones (AHLs) as quorum sensing (QS) signals to coordinate their behavior at the population level, including pathogens...
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The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
In recent years it has become clear that the production of N-acyl homoserine lactones (N-AHLs) is widespread in Gram-negative bacteria. These molecules act as diffusible chemical communication signals (bacterial pheromones) which regulate diverse physiological processes including bioluminescence, an …
快飞留学为您解读2020年QS本科大学排名、本科专业大学排名。快飞留学凭借权威独创的采矿工程专业海外世界院校数据库,提供最全面的QS采矿工程专业大学院校相关排名列表。
快飞留学为您解读2020年QS本科大学排名、本科专业大学排名。快飞留学凭借权威独创的管理学硕士专业海外世界院校数据库,提供最全面的QS管理学硕士专业大学院校相关排名列表。
Accepted name: acyl-homoserine-lactone acylase. Reaction: an N-acyl-L-homoserine lactone + H2O = L-homoserine lactone + a carboxylate. Other name(s): acyl-homoserine lactone acylase; AHL-acylase; AiiD; N-acyl-homoserine lactone acylase; PA2385 protein; quorum-quenching AHL acylase; quorum-quenching enzyme; PvdQ; QuiP. Systematic name: N-acyl-L-homoserine-lactone amidohydrolase. Comments: Acyl-homoserine lactones (AHLs) are produced by a number of bacterial species and are used by them to regulate the expression of virulence genes in a process known as quorum-sensing. Each bacterial cell has a basal level of AHL and, once the population density reaches a critical level, it triggers AHL-signalling which, in turn, initiates the expression of particular virulence genes. Plants or animals capable of degrading AHLs would have a therapeutic advantage in avoiding bacterial infection as they could prevent AHL-signalling and the expression of virulence genes in quorum-sensing bacteria. This ...
Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones (Cynthia H. Collins, Frances H. Arnold and Jared R. Leadbetter) Molecular Microbiology 2005 Acyl-homoserine lactone acylase from Ralstonia strain XJ12B represents a novel and potent class of quorum-quenching enzymes (Yi-Han Lin, Jin-Ling Xu, Jiangyong Hu, Lian-Hui Wang, Say Leong, Jared Renton Leadbetter and Lian-Hui Zhang) Molecular Microbiology 2003 Quorum Sensing and Quorum-quenching enzymes (Yi-Hu Dong and Lian-Hui Zhang) Journal of Microbiology 2005 AhlD, an N-acylhomoserine lactonase in Arthrobacter sp., and predicted homologues in other bacteria (Park, et. al) Microbiology 2003 AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora PNAS 2000 The molecular structure and catalytic mechanism of a quorum-quenching N-acyl-L-homoserine lactone hydrolase (Kim et al.) PNAS 2005 Communications blackout? Do ...
Some bacteria use pheromones (N-acylhomoserine lactones or AHLs) to determine their population density. Salmonella does not make AHLs but it can detect the AHLs produced by other bacteria. We hypothesized that Salmonella would detect AHLs made by the normal intestinal flora and use this information to adjust its gene expression accordingly. Surprisingly, we discovered that the normal gut flora does not make AHLs and instead, Salmonella is detecting the AHL production of other pathogens in the gut. Salmonella can detect the AHL production of Aeromonas hydrophila in turtles and Yersinia enterocolitica in mice. We are characterizing this same AHL detection system in E. coli, Klebsiella, Enterobacter and other closely related organisms.. 2. Coordination of metabolism and virulence by SirA and CsrA ...
Functional polymers that selectively recognize target compounds are developed by imprinting polymerization. In the present paper, two different dipyrromethanes, 5-phenol-dipyrromethane (5-pOH-DP) and 5-phenyl-dipyrromethane (5-ph-DP), are synthetized and investigated to develop conductive molecularly imprinted polymer (cMIP) sensors. As target molecules, two homoserine lactone derivatives were templated by an electrochemically driven polymerization process. Acyl-homoserine lactones (AHLs), also called homoserine lactones (HS), are a class of signaling molecules involved in bacterial quorum sensing (QS), which is a strategy of coordination among bacteria mediated by population density. The preparation of cMIP from 5-pOH-DP and 5-ph-DP in the presence of acetyl-homoserine lactone (Acetyl-HS) or carboxybenzyl-homoserine lactone (Cbz-HS) was performed by cyclic voltammetry (CV). The cMIP selectivity and sensitivity were assessed by microgravimetry (QCM). Both series of measurements were performed with the
Supplementary MaterialsSupplementary Information 41598_2019_44236_MOESM1_ESM. of the virulence and biofilm formation of is usually a Gram-negative bacteria that is highly resistant to existing antibiotics and cause many opportunistic and nosocomial infections. In particular, chronic lung infections with are the major causes of mortality in cystic fibrosis (CF) patients6. has been shown to form biofilms in the CF lung, which increase bacterial resistance to antibiotics7, and also produce several virulence factors including elastase, rhamnolipid, and pyocyanin. Thus, the inhibition of virulence factor production and biofilm formation may be highly attractive for the prevention and treatment of infections8. QS in is usually tightly regulated by three main QS systems organized within a hierarchical way3. LasR-LasI and RhlR-RhlI make use of acyl homoserine lactones (AHLs) as signaling substances, while PqsR-PqsABCD uses 2-alkyl-4-quinolones. RhlI and LasI, the AHL synthases, synthesize ...
Sigma-Aldrich offers abstracts and full-text articles by [Danielle M Stacy, Michael A Welsh, Philip N Rather, Helen E Blackwell].
Most studies of biofilms have focused on single species and on genes that control or are regulated by life on a surface. As more information is uncovered by studies of pure cultures, these data can be applied towards understanding the roles of specific genes in multispecies interactions. This chapter focuses mostly on multi-species interactions among oral bacteria in biofilms: a few single-species biofilms are featured to discuss responses to environmental signals, including signals generated by the occupants within the biofilm. Signals involved in cell-to-cell communication among biofilm cells include acyl homoserine lactones, oligopeptides, and autoinducer-2 (AI-2). Importantly, an optimal concentration of 4,5-dihydroxy-2,3-pentanedione (DPD) was critical for maximal biofilm development. One site where natural multispecies biofilms are unusually accessible is the tooth surface in the human oral cavity. We use a retrievable enamel chip model system that permits us to place three pieces of enamel side
Bijtenhoorn P, Schipper C, Hornung C, Quitschau M, Grond S, Weiland N, Streit W R (2011); J Biotechnol., 155(1):86-94. doi: 10.1016/j.jbiotec.2010.12.016. ...
The presence of charcoal in soil triggers a range of biological effects that are not yet predictable, in part because it interferes with the functioning of chemical signals that microbes release into their environment to communicate. We do not fully understand the mechanisms by which charcoal alters the biologically available concentrations of these intercellular signals. Recently, charcoal has been shown to sorb the signaling molecules that microbes release, rendering them ineffective for intercellular communication. Here, we investigate a second, potentially more important mechanism of interference: signaling-molecule hydrolysis driven by charcoal-induced soil pH changes. We examined the effects of 10 charcoals on the bioavailable concentration of an acyl-homoserine lactone (AHL) used by many Gram-negative bacteria for cell-cell communication. We show that charcoals decrease the level of bioavailable AHL through sorption and pH-dependent hydrolysis of the lactone ring. We then built a ...
One major microbiological problem is the widespread antibiotic resistance. There is an urgent need for new antibiotics and ways to treat multi-drug-resistant infections. Inhibition of bacterial quorum sensing (QS) systems could be an effective alternative in a smuch as they regulate a broad spectrum of cell functions, including, virulence factor production, biofilm organisation and motility. Influx and efflux bacterial systems involved in quorum sensing (QS) are known to depend on the proton motive force (PMF). Thus, a new series of 12 trifluoromethyl ketones (TFs) known to inhibit the PMF, was investigated for effects on the efflux pump of a QS responding bacterium, for its subsequent effect on the response to a QS signal and its direct inhibition of the response to a QS signal ...
Looking for the definition of AHLS? What does AHLS stand for? Find out it here! 13 meanings for AHLS abbreviations and acronyms on acronymsandslang.com The Worlds most comprehensive acronyms and slang dictionary!
Our project harnesses the use of quorum sensing to prevent bacteria from activating pathogenic factors triggered by AHLs. We developed a model system with Allivibrio fisheri. A. fisheri luminesces when activated by AHLs, which is a much safer alternative to pathogenic bacteria. We grew up our E. coli with Vibrio fisheri, and showed that the recombinant E. coli could detect V. fisheris AHLs, and inhibit V. fisheri from activating its quorum response genes by not producing light.The detection system then secretes factors that reduce the concentration of AHLs in the environment, thereby inhibiting the bacteria that use AHLs from communicating with each other. This is useful in preventing pathology from bacteria. When a threshold level of AHLs is reached in pathogenic bacteria, the signals activate transcription of pathogenic factors such as biofilm formation and host invasion signals (Raffa et al, 2005). We propose that disrupting the AHLs will inhibit these bacteria from creating biofilms or ...
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During this shaking period label chloroform-resistant tubes or 96 well plate with sample names and dilutions For ease of handling large numbers of eppendorf tubes, we load them in a rack, wrap saran wrap around the tubes + rack, secure the ends with packing tape, and shake the entire rack in the 37°C shaker. Saran wrap can be easily cut off using scissors at the end of the experiment ...
4G8D: High-resolution structures of AidH complexes provide insights into a novel catalytic mechanism for N-acyl homoserine lactonase
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The Dynamic Gallery of Thoughts, The Symmetry of I, the Circle of O ve A.M.G.O.D. alb mleriyle doksanlar n ikinci yar s nda ad ndan s z ettiren ve senfonik black metal olarak ba lad kariyerini sonradan end striyel metale eviren Fin grup …AND OCEANS, 2002′deki Cypher n ard ndan yeni alb m i in Kas m 2019′da st dyoya girmi ti.. ...
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Maryam Ebadi, Haniyeh Akhlaghi, Mohammad Mahdi Zamani, Hani Beheshti, Hassan Abolhassani, Ahmad Ayadi, Jaleh Dezdar, Seyedeh Hamideh Mortazzavi, Gholam Reza Karami, Morteza Izadi ...
TY - JOUR. T1 - N-acyl-L-homoserine lactones are inter-kingdom signalling molecules controlling quorum sensing in the Gram-negative bacteria and priming plants increasing stress adaptation. AU - Moshynets, O.. AU - Babenko, L.. AU - Karakhim, S.. AU - Kharkhota, M.. AU - Kosakivska, I.. AU - Rogalsky, S.. AU - Shcherbatiuk, M.. AU - Suslova, O.. AU - Spiers, A.. PY - 2017/9. Y1 - 2017/9. U2 - 10.1111/febs.14174. DO - 10.1111/febs.14174. M3 - Meeting Abstract. VL - 284. SP - 358. EP - 358. JO - FEBS Journal. JF - FEBS Journal. SN - 1742-464X. IS - S1. T2 - 42nd Congress of the Federation-of-European-Biochemical-Societies (FEBS) on From Molecules to Cells and Back. Y2 - 10 September 2017 through 14 September 2017. ER - ...
The recent rapid growth in the biofilm field has spawned a number of new strategies for controlling biofilms. Below are descriptions of a few of these emergent strategies.. For some years it has been known that bacteria communicate with each other via diffusible signal molecules in a process termed quorum sensing. The discovery that quorum sensing regulates biofilm formation opens the door to interdicting normal biofilm development through the use of quorum sensing inhibitors. This strategy of jamming communication is now moving towards application. One example of such inhibitors are the brominated furanones that block quorum sensing by acyl homoserine lactones, signal molecules used by Gram-negative bacteria. These furanones were first isolated from a marine algae and are thought to be part of the plants natural defense against microbial biofouling. Furanone-based quorum sensing inhibitors have been shown to increase antibiotic sensitivity of Pseudomonas aeruginosa biofilms and improve ...
Bacteria can coordinate and synchronize activities through a cell density-dependent regulatory mechanism called quorum sensing (QS). Bacteria can measure their population by the synthesis, secretion, and perception of QS signal molecules to regulate specific gene expression when the population reaches a critical threshold. QS participates in various microbial processes such as marine organism bioluminescence, bacterial biofilm formation, and virulence factor expression. The use of QS systems mediated by N-acyl-homoserine lactones and autoinducer-2 has been recently recognized as a promising regulatory approach in environmental science and technology that can intrinsically promote the profound comprehension of wastewater treatment from a microbiology perspective ...
1.THE GREEN FLUORESCENT PROTEIN - Annual Review of Biochemistry, 67(1):509.. 2.Tian T, Burrage K (2006) Stochastic models for regulatory networks of the genetic toggle switch. Proceedings of the National Academy of Sciences 103: 8372 -8377.. 3.Basu S, Mehreja R, Thiberge S, Chen M, Weiss R (2004) Spatiotemporal control of gene expression with pulse-generating networks. Proceedings of the National Academy of Sciences of the United States of America 101: 6355 -6360.. 4.Nilsson P, Olofsson A, Fagerlind M, Fagerström T, Rice S, u. a. (2001) Kinetics of the AHL Regulatory System in a Model Biofilm System: How Many Bacteria Constitute a Quorum? Journal of Molecular Biology 309: 631-640.. 5.Schaefer AL, Val DL, Hanzelka BL, Cronan JE, Greenberg EP (1996) Generation of cell-to-cell signals in quorum sensing: acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein. Proc. Natl. Acad. Sci. U.S.A 93: 9505-9509.. 6.Kaplan HB, Greenberg EP (1985) Diffusion of autoinducer is ...
Many common bacterial pathogens use chemical signals to coordinate group behaviors. In the canonical quorum sensing (QS) system of V. fischeri, a synthase, LuxI, produces an N-acyl homoserine lactone (AHL) that is recognized by a receptor, LuxR, at high signal density. LuxR is a transcription factor that regulates bioluminescence and other group behaviors at high population density in an AHL dependent manner. SdiA is a LuxR homolog found in Escherichia coli and Salmonella enterica serovar typhimurium. SdiA does not have a corresponding synthase, however, and these species do not produce AHL signals. SdiA does not respond to population density, but rather has been shown to respond to AHLs produced by other bacterial species in its environment. SdiA regulates many aspects of pathogenesis including directing the location of the infection, survival in the mammalian digestive system, and the production of virulence factors. Accordingly, modulation of SdiA activity might be a useful anti-virulence ...
The goal of this project is to decipher the quorum sensing (cell-to-cell signaling) abilities of Shewanella oneidensis MR-1, a Gram-negative bacterium well known for its ability to use geologic substrates, such as Fe and Mn oxides, for respiratory purposes. Overall our results show that S. oneidensis cannot utilize either an acyl-homoserine lactone (AHL) or AI-2 quorum sensing signal, despite previous work that indicated that it produced an AHL that would enhance it ability to growth in certain anaerobic environments. Using a variety of quorum sensing signal sensors, no evidence could be found that S. oneidensis has a typical AHL signal. An in silco analysis of the genome also produced little evidence that S. oneidensis has the genes to accept or relay an AHL signal. S. oneidensis can produce a luminescence response in the AI-2 reporter strain, Vibrio harveyi MM32. This luminescence response is abolished upon deletion of luxS, the gene responsible for catalyzing AI-2. Deletion of luxS also ...
Introduction: Lumichrome, a photodegradation product of riboflavin, is an endogenous compound in humans. The compound is more photostable and a more efficient photogenerator of singlet oxygen than riboflavin. It absorbs radiation in the UVA and blue light region, which can be an advantage in aPDT of superficial infections.. Aims and objectives: The aim of this study was to investigate the aPDT effect of various lumichrome pharmaceuticals formulations on bacteria in vitro.. Material & methods: Solutions of lumichrome (10-5 - 10-3M) were prepared in plain PBS, in PBS-cosolvents solutions or in formulations containing cyclodextrins or polyoxamers. Supersaturated solutions of lumichrome in PBS were prepared via the cosolvent and solvent evaporation method. The antibacterial effect of selected lumichrome preparations was studied in vitro against plaktonic Gram-positive and Gram-negative bacteria. The light dose used was ≈ 13 J/cm² and the lamp emitted mainly in the range 340-440 nm.. Results & ...
LuxI and LuxR are key factors that drive quorum sensing (QS) in bacteria through secretion and perception of the signaling molecules e.g. N-Acyl homoserine lactones (AHLs). The role of these proteins is well established in Gram-negative bacteria for intercellular communication but remain under-explored in Gram-positive bacteria where QS peptides are majorly responsible for cell-to-cell communication. Therefore, in the present study, we explored conservation, potential function, topological arrangements and evolutionarily aspects of these proteins in Gram-positive bacteria. Putative LuxI/LuxR containing proteins were retrieved using the domain-based strategy from InterPro v62.0 meta-database. Conservational analyses via multiple sequence alignment and domain showed that these are well conserved in Gram-positive bacteria and possess relatedness with Gram-negative bacteria. Further, Gene ontology and ligand-based functional annotation explain their active involvement in signal transduction ...
Many Gram-negative bacteria employ N-acylhomoserine lactones (AHLs) as quorum-sensing (QS) signal molecules to regulate virulence expression in a density-dependent manner. Quorum quenching (QQ) via enzymatic inactivation of AHLs is a promising strategy to reduce bacterial infections and drug resistance. Herein, a thermostable AHL lactonase (AidB), which could degrade different AHLs, with or without a substitution of carbonyl or hydroxyl at the C-3 position, was identified from the soil bacterium Bosea sp. strain F3-2. Ultrahigh-performance liquid chromatography analysis demonstrated that AidB is an AHL lactonase that hydrolyzes the ester bond of the homoserine lactone (HSL) ring. AidB was thermostable in the range 30 to 80°C and showed maximum activity after preincubation at 60°C for 30 min. The optimum temperature of AidB was 60°C, and the enzyme could be stably stored in double-distilled water (ddH2O) at 4°C or room temperature. AidB homologs were found only in Rhizobiales and ...
Despite the discovery of the first N-acyl homoserine lactone (AHL) based quorum sensing (QS) in the marine environment, relatively little is known about the abundance, nature and diversity of AHL QS systems in this diverse ecosystem. Establishing the prevalence and diversity of AHL QS systems and how they may influence population dynamics within the marine ecosystem, may give a greater insight into the evolution of AHLs as signaling molecules in this important and largely unexplored niche. Microbiome profiling of Stelletta normani and BD1268 sponge samples identified several potential QS active genera. Subsequent biosensor-based screening of a library of 650 marine sponge bacterial isolates identified 10 isolates that could activate at least one of three AHL biosensor strains. Each was further validated and profiled by Ultra-High Performance Liquid Chromatography Mass Spectrometry, with AHLs being detected in 8 out of 10 isolate extracts. Co-culture of QS active isolates with S. normani marine ...
definition of HBHL, what does HBHL mean?, meaning of HBHL, Hydroxybutanoyl Homoserine Lactone, HBHL stands for Hydroxybutanoyl Homoserine Lactone
Recent studies have identified a novel interkingdom signaling circuit, via plant signaling molecules, and a bacterial sub-family of LuxR proteins, bridging eukaryotes and prokaryotes. Indeed pivotal plant-bacteria interactions are regulated by the so called Plant Associated Bacteria (PAB) LuxR solo regulators that, although closely related to the quorum sensing (QS) LuxR family, do not bind or respond to canonical quorum sensing N-acyl homoserine lactones (AHLs), but only to specific host plant signal molecules. The large body of structural data available for several members of the QS LuxR family complexed with different classes of ligands (AHLs and other compounds), has been exploited to dissect the cartography of their regulatory domains through structure-based multiple sequence alignments, structural superimposition and a comparative analysis of the contact residues involved in ligand binding. In the absence of experimentally determined structures of members of the PAB LuxR solos subfamily, an
Quorum sensing regulation, mediated by N-acyl homoserine lactone signals, produced by strain Serratia plymuthica HRO-C48 isolated from the rhizosphere of oilseed rape, was found to be responsible for
Our body consists of 100 trillion cells that communicate with each other, receive signals from the outside world and react to them. A central role in this communication network is attributed to receiver proteins, called receptors, which are anchored at the cell membrane. There, they receive and transmit signals to the inside of the cell, where a cell reaction is triggered. In humans, G protein-coupled receptors (GPC receptors) represent the largest group of these receptor molecules, with around 700 different types. The research of the Frankfurt and Leipzig scientists focused on a GPC receptor that serves as a receptor for the neuropeptide Y in cells and is accordingly called the Y2 receptor. Neuropeptide Y is a messenger substance that primarily mediates signals between nerve cells, which is why Y2 receptors are mainly present in nerve cells and among other activities trigger the formation of new cell connections.. In the laboratory, the researchers engineered cells, which had approx. 300,000 Y2 ...
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CP001096.PE321 Location/Qualifiers FT CDS_pept complement(343120..343851) FT /codon_start=1 FT /transl_table=11 FT /locus_tag=Rpal_0323 FT /product=transcriptional regulator, LuxR family FT /note=PFAM: regulatory protein LuxR; Autoinducer-binding FT domain protein; KEGG: bbt:BBta_7112 putative N-acyl FT homoserine lactone transcriptional regulator, LuxR-like FT protein FT /db_xref=EnsemblGenomes-Gn:Rpal_0323 FT /db_xref=EnsemblGenomes-Tr:ACE98883 FT /db_xref=GOA:B3Q959 FT /db_xref=InterPro:IPR000792 FT /db_xref=InterPro:IPR005143 FT /db_xref=InterPro:IPR016032 FT /db_xref=InterPro:IPR036388 FT /db_xref=InterPro:IPR036693 FT /db_xref=UniProtKB/TrEMBL:B3Q959 FT /protein_id=ACE98883.1 FT /translation=MIVGEDQLWGRRALEFVDSVERLEAPALISRFESLIASCGFTAYI FT MAGLPSRNAGLPELTLANGWPRDWFDLYVSENFSAVDPVPRHGATTVYPFVWSDAPYDR FT DRDPAAHRVMTRAAEFGLVEGYCIPLHYDDGSAAISMAGKDPDLSPAARGAMQLVSIYA FT HSRLRALSRPKPIRRNRLTPRECEILQWAAQGKTAWEISVILCITERTVKFHLIEAARK FT LDAANRTAAVAKALTLGLIRL atgatcgtcg ...
This textbook provides a comprehensive overview of the specific signaling pathways on a variety of human tissues. You understand what makes a tissue specific and depict key signaling pathways for tissue-specific functions in the plethora of signals, signals receptors, transducers and effectors.
Visit Healthgrades for information on Dr. Hani Salha, MD Find Phone & Address information, medical practice history, affiliated hospitals and more.
See what Yeni Albüm İndir (canansihirdar) has discovered on Pinterest, the worlds biggest collection of everybodys favorite things.
Z-D-Homoserine Lactone chemical properties, What are the chemical properties of Z-D-Homoserine Lactone 41088-89-5, What are the physical properties of Z-D-Homoserine Lactone ect.
Monsanto, with an annual turnover of around 13.5 billion US dollars last year, is the largest seed company in the world. Based out of St. Luis in the USA, it also leads the Genetically Modified seed sector globally. Monsanto in India exists as Monsanto India Ltd, Monsanto Holding Pvt Ltd, Mahyco-Monsanto Biotech, besides holding 26% share in the Indian seed company Mahyco.
Quorum ceiling fans manufacturers already over 30 years high quality fans. They are known for the wide choice of types, styles, prices and great designs.
One of PolySciTech’s latest product offerings is Akanocure stereotetrad lactones (https://akinainc.com/polyscitech/products/akanocure/index.php) for
Long story short, i am a long term patient of the brai2n clinic in Antwerp. Dirk De Ridder was my physician. I had done a TMS test which was negative...
İzmir Tınaztepe Üniversitesi geleceğe öğrenci yetiştirmektedir. Tecrübeli kadrosu ile eğitime yeni bir yön çizmeyi hedeflemektedir. Bireyleri gerçek iş hayatına hazırlamayı hedefleyen eğitim kurumudur.
3P The reaction product of ethylene oxide with acyl chlorides in the presence of sodium iodide is a complex iodoethyl ester: ( ... reactions of ethylene oxide with compounds containing active methylene group leads to the formation of butyrolactones: Ethylene ...
Ravinder S. Reddy And K. Saravanan and Pradeep Kumar*, An efficient approach to ?-alkylidene ?-butyrolactones : Application to ... Pradeep Kumar and A.K. Mukerjee, Acylation of primary amines by O-acyl oximes, Synthesis., 1979, 726 (1979). ... butyrolactones. Its relevance to photo- stability of Fredericamycin A, J.Chem.Soc.Chem. Commun., 1993, 870 (1993). ...
Acyl-Butyrolactones Medicine & Life Sciences 89% * Cattle Medicine & Life Sciences 70% * Rumen Medicine & Life Sciences 43% ... EHEC harbors SdiA, a regulator that senses acyl-homoserine lactones (AHLs) produced by other bacteria. Here,we show that SdiA ... EHEC harbors SdiA, a regulator that senses acyl-homoserine lactones (AHLs) produced by other bacteria. Here,we show that SdiA ... EHEC harbors SdiA, a regulator that senses acyl-homoserine lactones (AHLs) produced by other bacteria. Here,we show that SdiA ...
The discrepancies in chemical framework Among the many γ-butyrolactones are classified as the size and branching from the acyl ...
Acyl homoserine lactones *N-acyl amines *Gamma butyrolactones *Oxolanes *Secondary carboxylic acid amides ... Belongs to the class of organic compounds known as n-acyl-alpha amino acids and derivatives. N-acyl-alpha amino acids and ... belongs to the class of organic compounds known as n-acyl-alpha amino acids and derivatives. N-acyl-alpha amino acids and ... derivatives are compounds containing an alpha amino acid (or a derivative thereof) which bears an acyl group at its terminal ...
C. B. Rao, N. Zhang, J. Hu, Y. Wang, Y. Liang, R. Zhang,* J.Yuan, D.Dong,* Tf2O-Mediated Cyclization of α-Acyl-β-(2- ... A Novel Entry to γ-Butyrolactones, Synthesis 2012, 1679-1685. ... Diones from α-Acyl-α-Carbamoyl Ketene-S,S-Acetals, Synthesis ... J. Yang, D. Xiang, R. Zhang, N. Zhang,* Y. Liang, X. Xin, D. Dong,* POCl3-Mediated Reaction of 1-Acyl-1-Carbamoyl Oximes: A New ... M. Yu, Q. Zhang, G. Li, J. Yuan, N. Zhang,* R. Zhang, Y. Liang, D. Dong,* Copper-Nitrene Triggered Annulation of α-Acyl ...
Acyl-Butyrolactones [D02.540.232] * Dehydroascorbic Acid [D02.540.260] * Eplerenone [D02.540.383] * Macrolides [D02.540.505] ...
Acyl-Butyrolactones. *Adult. *Aerosols. *Analgesics. *Anesthesia, Spinal. *Anesthetics, Local. *Animals. *Anthrax. *Antibodies ...
Beal, J., Telmer, C. A., Vignoni, A., Boada, Y., Baldwin, G. S., Hallett, L., Lee, T., Selvarajah, V., Billerbeck, S., Brown, B., Cai, G. N., Cai, L., Eisenstein, E., Kiga, D., Ross, D., Alperovich, N., Sprent, N., Thompson, J., Young, E. M., Endy, D., & 1 othersHaddock-Angelli, T., 2022, In: Synthetic Biology. 7, 1, ysac010.. 研究成果: Article › 査読 ...
La bièrra sembla èsser apareguda ambé lei culturas de cerealas en Mesopotamia Anciana. Pasmens, en causa de la manca de donadas, la data precisa es desconeguda. D'un biais generau, es situada vèrs 6 000 avC car lei tecnologias dau periòde (mestritge de la produccion d'òrdi e de blat[1], terralhas capablas de protegir una bevenda alcoolizada...) permetián alora son existéncia[2]. La premiera traça clarament atestada data dau milleni IV avC e foguèt descubèrta en Sumèr. Dicha « sikaru », fasiá partida dau manjar de la vida vidante e sa fabricacion èra protegida per la divessa Ninkasi[3]. Èra producha a partir de fogassas d'òrdi ò d'espèuta trempadas dins l'aiga per entraïnar la fermentacion. D'autreis ingredients i èran de còps aponduts (mèu, espècias...) per ne melhorar lo gost. A partir de Mesopotamia, la bièrra se difusèt vèrs lei regions vesinas[4]. Pasmens, es tanben possible que dins certaneis endrechs, de descubèrtas independentas aguèron luòc. Que mecanisme ...
Bel Air (or Bel-Air) is a neighborhood in the Los Angeles Westside, in the foothills of the Santa Monica Mountains. It was founded in 1923. It is a lightly populated residential district. The people are older, well-educated, and have high incomes. ...
The follaein 2 pages is in this categerie, oot o 2 awthegither. ...
Schooda (dt.: Schotter, engl.: crushed stone (kinstle), gravel (natiale)) san kloane Breckal bzw. Keandln vo Stoana und Fejsn, wo natiale oda kinstle entstondn san und gressa wia 2 mm san. Wonns kloana san, is's a Sond. A Schooda wo natiale entstondn is, nennd ma aa Kisl. A Schooda wo kinstle brocha is, nennd ma Splitt. ...
Gallout a reer implijout an testennoù zo dindan an Aotre-Implijout Creative Commons Dereiñ/Kenrannañ diouzh an hevelep divizoù; divizoù ouzhpenn a c'hall bezañ ivez. Gwelet an Doareoù Implijout evit gouzout hiroc'h ...
picha_ya_satelite = Localización de la provincia de Alicante. ...
Ang teksto puyde magamit ubos sa Creative Commons Attribution-ShareAlike License; puyde madugangan ang mga termino. Tan-awa ang Mga Termino sa Paggamit para sa mga detalye ...
Ta Lanreunan (Frangish: Laurenan, Galloish: Laor'nan) ny chommyoon 'sy département Frangagh Aodoù-an-Arvor 'sy Vritaan ayns sheear hwoaie ny Frank. Ta'n ennym Frangish Laurenanais currit er cummaltee yn valley. ...
Maldiv, an form long Répiblik dé Maldiv (an dhivehi : ދިވެހިރާއްޖޭގެ ޖުމްހޫރިއްޔާ, Dhivehi Raa'jeyge Jumhooriyya), sa roun péyi di Lazi di Sid konstitchwé di 1 199 zil, don 202 ki gen moun ki ka rété, sitchwé o sid-wès di sou-kontinan endyen. ...
Si Papa Leó III(750 - 12 Hunyo 816) ang Papa ng Simbahang Katoliko Romano mula 795 CE hanggang sa kanyang kamatayan noong 816 CE. Siya ay iningatan ni Charlemagne mula sa kanyang mga kaaway sa Roma. Kanyang kalaunang pinakalakas ang posisyon ni Charlemagne sa pamamagitan ng pagkorona rito bilang Banal na Emperador Romano. ...
Welcome on my talk page, you can also leave a message on my Dutch talk page for a fast response ...
This template displays discharge measurement units used within various Category:Geobox templates, automatically converting between metric/imperial values. The input must be unformatted numeric values (i.e. without commas, the commas are automatically added to the output). ...
Hi Osmeña usa nga abogado ngan editor hin periodiko ugsa hiya mahibulig ha lokal nga mga politika. Nagtikáng hiya komo konsehal. Han 1904, an Amerikano nga kolonyal nga administrasyon naghimo ha iya nga gobernador han Sugbo o Cebu. Kaatapos hin duha ka tuig, na-elihir hiya nga gobernador han Sugbo. Samtang gobernador hiya, nagdalagan hiya dida han pinili-ay para han syahan nga Kapulongan Pilipinhon o Philippine Assembly han 1907 ngan napili hiya nga Speaker hadton nga kapulongan. Hi Osmeña 29 pa la an edad han kahimo niya nga gihataasi in ranggo nga opisyal Pilipino hadto nga panahon. Hiya ngan in usa liwat nga politiko, nga hi Manuel L. Quezon han Tayabas, nagtukod han Partido Nacionalista komo baton han Partido Federalista han mga politiko-ha-Manila. An duha nagkaada hin pagkaribal para hin kadominante ha politika tikang hini nga panahon. ...
An samar da dukkan muƙaloli a ƙarƙashin lasisin Creative Commons Attribution/Share-Alike; tana yiwuwa wasu ƙa'idoji su yi tasiri. Ku duba Ka'idojin Amfani na Gidauniyar Wikimedia domin ƙarin bayani ...
Ti Senado ti Filipinas ket ti akin-ngato a benneg ti bikameral a lehislatura ti Filipinas (Kongreso ti Filipinas). Buklen ti 24 a senador ti Senado, a mabutosan iti maysa a nailian nga eleksion. Saan nga ibagian ti maysa a senador ti maysa a partikular a geograpikal a distrito. Agpaay dagiti senador iti 6 a tawen a termino. Kaguadua (12) dagiti senador ti mabutosan iti kada tallo a tawen. ...
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თე ხასჷლაშ ბოლო რედაქტირაფაშ თარიღი: 22:02, 31 კვირკვე 2016 ...
image = Aberdeen, William Wallace close-up - geograph.org.uk - 598093. ...
Të gjitha materialet që gjenden në këtë faqë janë të mbrojtura nga Creative Commons Attribution/Share-Alike License;. Shiko Terms of Use për më shumë informacione ...
13:2613:26, 20 जनवरी 2022 अन्तर इतिहास −165‎ वृन्दावन ‎ wrong images removed, ‎Kusum Sarovar is located outside of Vrindavan ...
Acyl-Butyrolactones 24% * Quorum Sensing 20% * quinone 19% * Oryza 19% * Republic of Korea 19% ...
Beal, J., Telmer, C. A., Vignoni, A., Boada, Y., Baldwin, G. S., Hallett, L., Lee, T., Selvarajah, V., Billerbeck, S., Brown, B., Cai, G. N., Cai, L., Eisenstein, E., Kiga, D., Ross, D., Alperovich, N., Sprent, N., Thompson, J., Young, E. M., Endy, D., & 1 othersHaddock-Angelli, T., 2022, In: Synthetic Biology. 7, 1, ysac010.. 研究成果: Article › 査読 ...
Acyl-Butyrolactones Medicine & Life Sciences 89% * Cattle Medicine & Life Sciences 70% * Rumen Medicine & Life Sciences 43% ... EHEC harbors SdiA, a regulator that senses acyl-homoserine lactones (AHLs) produced by other bacteria. Here,we show that SdiA ... EHEC harbors SdiA, a regulator that senses acyl-homoserine lactones (AHLs) produced by other bacteria. Here,we show that SdiA ... EHEC harbors SdiA, a regulator that senses acyl-homoserine lactones (AHLs) produced by other bacteria. Here,we show that SdiA ...
Acyl-Butyrolactones. *Adult. *Aerosols. *Analgesics. *Anesthesia, Spinal. *Anesthetics, Local. *Animals. *Anthrax. *Antibodies ...
Rational Design of a Tailored N-Heterocyclic Carbene CatalystA highly selective NHC-catalyzed synthesis of γ-butyrolactones ... Asymmetric Homoenolate Additions to Acyl Phosphonates through Rational Design of a Tailored N-Heterocyclic Carbene Catalyst. ... A highly selective NHC-catalyzed synthesis of γ-butyrolactones from the fusion of enals and α-ketophosphonates has been ... Home » Asymmetric Homoenolate Additions to Acyl Phosphonates through Rational Design of a Tailored N-Heterocyclic Carbene ...
Acyl-Butyrolactones [D02.540.232] * Dehydroascorbic Acid [D02.540.260] * Eplerenone [D02.540.383] * Macrolides [D02.540.505] ...
The discrepancies in chemical framework Among the many γ-butyrolactones are classified as the size and branching from the acyl ...
Acyl homoserine lactones *N-acyl amines *Gamma butyrolactones *Oxolanes *Secondary carboxylic acid amides ... Belongs to the class of organic compounds known as n-acyl-alpha amino acids and derivatives. N-acyl-alpha amino acids and ... belongs to the class of organic compounds known as n-acyl-alpha amino acids and derivatives. N-acyl-alpha amino acids and ... derivatives are compounds containing an alpha amino acid (or a derivative thereof) which bears an acyl group at its terminal ...
3P The reaction product of ethylene oxide with acyl chlorides in the presence of sodium iodide is a complex iodoethyl ester: ( ... reactions of ethylene oxide with compounds containing active methylene group leads to the formation of butyrolactones: Ethylene ...
Ravinder S. Reddy And K. Saravanan and Pradeep Kumar*, An efficient approach to ?-alkylidene ?-butyrolactones : Application to ... Pradeep Kumar and A.K. Mukerjee, Acylation of primary amines by O-acyl oximes, Synthesis., 1979, 726 (1979). ... butyrolactones. Its relevance to photo- stability of Fredericamycin A, J.Chem.Soc.Chem. Commun., 1993, 870 (1993). ...
C. B. Rao, N. Zhang, J. Hu, Y. Wang, Y. Liang, R. Zhang,* J.Yuan, D.Dong,* Tf2O-Mediated Cyclization of α-Acyl-β-(2- ... A Novel Entry to γ-Butyrolactones, Synthesis 2012, 1679-1685. ... Diones from α-Acyl-α-Carbamoyl Ketene-S,S-Acetals, Synthesis ... J. Yang, D. Xiang, R. Zhang, N. Zhang,* Y. Liang, X. Xin, D. Dong,* POCl3-Mediated Reaction of 1-Acyl-1-Carbamoyl Oximes: A New ... M. Yu, Q. Zhang, G. Li, J. Yuan, N. Zhang,* R. Zhang, Y. Liang, D. Dong,* Copper-Nitrene Triggered Annulation of α-Acyl ...
Benzoins via an acyl anion equivalent. Novel one-pot preparation of benzo[b]furans via benzoins using hydriodic acid ... Substituted .gamma.-butyrolactones. Part 32. Ring construction using 3-(arylmethylene)-2,4(3H,5H)-furandione: synthesis of ... I. Preparation of α-Substituted γ-Butyrolactones by Condensation of γ-Butyrolactone with Aldehydes. Hydrogenation of the ...
Zhang, C.; Moran, E.J.; Woiwade, T.F.; Short, K.M.; Mjalli, A.M.M. Synthesis of tetra substituted imidazoles via α-(N-acyl-N- ... 5trisubstituted γ-butyrolactones. Synthesis, 2007, 3195-3200.. [http://dx.doi.org/10.1055/s-2007-990781] (e) Potewar, T.M.; ...

No data available that match "acyl butyrolactones"


  • Salmonella is an important foodborne pathogen, and it is unable to produce the quorum sensing signaling molecules called acyl-homoserine lactones (AHLs). (nih.gov)
  • A treatment of normal and cancer cells with C12 or other N-acyl homoserine lactones (AHLs) results in rapid translocation of MVP into lipid raft (LR) membrane fractions. (bgu.ac.il)
  • The organism has two acyl-homoserine lactone (HSL)-mediated QS systems, LasR/LasI and RhlR/RhlI. (elsevier.com)