Acute-Phase Proteins: Proteins that are secreted into the blood in increased or decreased quantities by hepatocytes in response to trauma, inflammation, or disease. These proteins can serve as inhibitors or mediators of the inflammatory processes. Certain acute-phase proteins have been used to diagnose and follow the course of diseases or as tumor markers.Haptoglobins: Plasma glycoproteins that form a stable complex with hemoglobin to aid the recycling of heme iron. They are encoded in man by a gene on the short arm of chromosome 16.Acute-Phase Reaction: An early local inflammatory reaction to insult or injury that consists of fever, an increase in inflammatory humoral factors, and an increased synthesis by hepatocytes of a number of proteins or glycoproteins usually found in the plasma.OrosomucoidSerum Amyloid A Protein: An ACUTE PHASE REACTION protein present in low concentrations in normal sera, but found at higher concentrations in sera of older persons and in patients with AMYLOIDOSIS. It is the circulating precusor of amyloid A protein, which is found deposited in AA type AMYLOID FIBRILS.alpha 1-Antichymotrypsin: Glycoprotein found in alpha(1)-globulin region in human serum. It inhibits chymotrypsin-like proteinases in vivo and has cytotoxic killer-cell activity in vitro. The protein also has a role as an acute-phase protein and is active in the control of immunologic and inflammatory processes, and as a tumor marker. It is a member of the serpin superfamily.Turpentine: The concrete oleoresin obtained from Pinus palustris Mill. (Pinaceae) and other species of Pinus. It contains a volatile oil, to which its properties are due, and to which form it is generally used. (Dorland, 28th ed) Turpentine is used as a solvent and an experimental irritant in biomedical research. Turpentine toxicity is of medical interest.alpha 1-Antitrypsin: Plasma glycoprotein member of the serpin superfamily which inhibits TRYPSIN; NEUTROPHIL ELASTASE; and other PROTEOLYTIC ENZYMES.C-Reactive Protein: A plasma protein that circulates in increased amounts during inflammation and after tissue damage.Serum Amyloid P-Component: Amyloid P component is a small, non-fibrillar glycoprotein found in normal serum and in all amyloid deposits. It has a pentagonal (pentaxin) structure. It is an acute phase protein, modulates immunologic responses, inhibits ELASTASE, and has been suggested as an indicator of LIVER DISEASE.Interleukin-6: A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.Fibrinogen: Plasma glycoprotein clotted by thrombin, composed of a dimer of three non-identical pairs of polypeptide chains (alpha, beta, gamma) held together by disulfide bonds. Fibrinogen clotting is a sol-gel change involving complex molecular arrangements: whereas fibrinogen is cleaved by thrombin to form polypeptides A and B, the proteolytic action of other enzymes yields different fibrinogen degradation products.Mycoplasma hyosynoviae: A species of gram-negative bacteria isolated from the SYNOVIAL FLUID; LYMPH NODES; and MUCOUS MEMBRANE secretions in diseased SWINE. It causes nonsuppurative ARTHRITIS.alpha-Macroglobulins: Glycoproteins with a molecular weight of approximately 620,000 to 680,000. Precipitation by electrophoresis is in the alpha region. They include alpha 1-macroglobulins and alpha 2-macroglobulins. These proteins exhibit trypsin-, chymotrypsin-, thrombin-, and plasmin-binding activity and function as hormonal transporters.CeruloplasminBlood Proteins: Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.Dictyocaulus: Nematodes parasitic in the bronchi of herbivorous animals.Inflammation: A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.Serum Albumin: A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Interleukin-1: A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.Alpha-Globulins: Serum proteins that have the most rapid migration during ELECTROPHORESIS. This subgroup of globulins is divided into faster and slower alpha(1)- and alpha(2)-globulins.Xerophthalmia: Dryness of the eye surfaces caused by deficiency of tears or conjunctival secretions. It may be associated with vitamin A deficiency, trauma, or any condition in which the eyelids do not close completely.Protein Array Analysis: Ligand-binding assays that measure protein-protein, protein-small molecule, or protein-nucleic acid interactions using a very large set of capturing molecules, i.e., those attached separately on a solid support, to measure the presence or interaction of target molecules in the sample.Prealbumin: A tetrameric protein, molecular weight between 50,000 and 70,000, consisting of 4 equal chains, and migrating on electrophoresis in 3 fractions more mobile than serum albumin. Its concentration ranges from 7 to 33 per cent in the serum, but levels decrease in liver disease.Actinobacillus Infections: Infections with bacteria of the genus ACTINOBACILLUS.Housing, AnimalAlbumins: Water-soluble proteins found in egg whites, blood, lymph, and other tissues and fluids. They coagulate upon heating.Lipocalins: A diverse family of extracellular proteins that bind to small hydrophobic molecules. They were originally characterized as transport proteins, however they may have additional roles such as taking part in the formation of macromolecular complexes with other proteins and binding to CELL SURFACE RECEPTORS.Complement C3: A glycoprotein that is central in both the classical and the alternative pathway of COMPLEMENT ACTIVATION. C3 can be cleaved into COMPLEMENT C3A and COMPLEMENT C3B, spontaneously at low level or by C3 CONVERTASE at high level. The smaller fragment C3a is an ANAPHYLATOXIN and mediator of local inflammatory process. The larger fragment C3b binds with C3 convertase to form C5 convertase.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Biological Markers: Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.Transferrin: An iron-binding beta1-globulin that is synthesized in the LIVER and secreted into the blood. It plays a central role in the transport of IRON throughout the circulation. A variety of transferrin isoforms exist in humans, including some that are considered markers for specific disease states.Complement C4: A glycoprotein that is important in the activation of CLASSICAL COMPLEMENT PATHWAY. C4 is cleaved by the activated COMPLEMENT C1S into COMPLEMENT C4A and COMPLEMENT C4B.Actinobacillus pleuropneumoniae: A species of gram-negative, facultatively anaerobic coccobacillus-shaped bacteria that has been isolated from pneumonic lesions and blood. It produces pneumonia with accompanying fibrinous pleuritis in swine.Tumor Necrosis Factor-alpha: Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.Lipopolysaccharides: Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)Swine Diseases: Diseases of domestic swine and of the wild boar of the genus Sus.Night Blindness: Failure or imperfection of vision at night or in dim light, with good vision only on bright days. (Dorland, 27th ed)Cytokines: Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.Vitamin A Deficiency: A nutritional condition produced by a deficiency of VITAMIN A in the diet, characterized by NIGHT BLINDNESS and other ocular manifestations such as dryness of the conjunctiva and later of the cornea (XEROPHTHALMIA). Vitamin A deficiency is a very common problem worldwide, particularly in developing countries as a consequence of famine or shortages of vitamin A-rich foods. In the United States it is found among the urban poor, the elderly, alcoholics, and patients with malabsorption. (From Cecil Textbook of Medicine, 19th ed, p1179)Blood Sedimentation: Measurement of rate of settling of erythrocytes in anticoagulated blood.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.STAT3 Transcription Factor: A signal transducer and activator of transcription that mediates cellular responses to INTERLEUKIN-6 family members. STAT3 is constitutively activated in a variety of TUMORS and is a major downstream transducer for the CYTOKINE RECEPTOR GP130.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Acute Disease: Disease having a short and relatively severe course.Cytokine Receptor gp130: A cytokine receptor that acts through the formation of oligomeric complexes of itself with a variety of CYTOKINE RECEPTORS.Receptors, Interleukin-6: Cell surface receptors that are specific for INTERLEUKIN-6. They are present on T-LYMPHOCYTES, mitogen-activated B-LYMPHOCYTES, and peripheral MONOCYTES. The receptors are heterodimers of the INTERLEUKIN-6 RECEPTOR ALPHA SUBUNIT and the CYTOKINE RECEPTOR GP130.Dexamethasone: An anti-inflammatory 9-fluoro-glucocorticoid.Carcinoma, Hepatocellular: A primary malignant neoplasm of epithelial liver cells. It ranges from a well-differentiated tumor with EPITHELIAL CELLS indistinguishable from normal HEPATOCYTES to a poorly differentiated neoplasm. The cells may be uniform or markedly pleomorphic, or form GIANT CELLS. Several classification schemes have been suggested.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Interleukins: Soluble factors which stimulate growth-related activities of leukocytes as well as other cell types. They enhance cell proliferation and differentiation, DNA synthesis, secretion of other biologically active molecules and responses to immune and inflammatory stimuli.Endotoxins: Toxins closely associated with the living cytoplasm or cell wall of certain microorganisms, which do not readily diffuse into the culture medium, but are released upon lysis of the cells.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Leukocyte Count: The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Recombinant Proteins: Proteins prepared by recombinant DNA technology.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Mice, Inbred C57BLCattle Diseases: Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus.Inflammation Mediators: The endogenous compounds that mediate inflammation (AUTACOIDS) and related exogenous compounds including the synthetic prostaglandins (PROSTAGLANDINS, SYNTHETIC).Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Statistics, Nonparametric: A class of statistical methods applicable to a large set of probability distributions used to test for correlation, location, independence, etc. In most nonparametric statistical tests, the original scores or observations are replaced by another variable containing less information. An important class of nonparametric tests employs the ordinal properties of the data. Another class of tests uses information about whether an observation is above or below some fixed value such as the median, and a third class is based on the frequency of the occurrence of runs in the data. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1284; Corsini, Concise Encyclopedia of Psychology, 1987, p764-5)Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Hepatocytes: The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules.Arthritis, Rheumatoid: A chronic systemic disease, primarily of the joints, marked by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Etiology is unknown, but autoimmune mechanisms have been implicated.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Monocytes: Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.Glycosylation: The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.Case-Control Studies: Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Sepsis: Systemic inflammatory response syndrome with a proven or suspected infectious etiology. When sepsis is associated with organ dysfunction distant from the site of infection, it is called severe sepsis. When sepsis is accompanied by HYPOTENSION despite adequate fluid infusion, it is called SEPTIC SHOCK.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Liver Neoplasms: Tumors or cancer of the LIVER.Membrane Glycoproteins: Glycoproteins found on the membrane or surface of cells.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Kinetics: The rate dynamics in chemical or physical systems.Reference Values: The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.Mice, Inbred BALB CReverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Neutrophils: Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Proteomics: The systematic study of the complete complement of proteins (PROTEOME) of organisms.Chagas Disease: Infection with the protozoan parasite TRYPANOSOMA CRUZI, a form of TRYPANOSOMIASIS endemic in Central and South America. It is named after the Brazilian physician Carlos Chagas, who discovered the parasite. Infection by the parasite (positive serologic result only) is distinguished from the clinical manifestations that develop years later, such as destruction of PARASYMPATHETIC GANGLIA; CHAGAS CARDIOMYOPATHY; and dysfunction of the ESOPHAGUS or COLON.Mucocutaneous Lymph Node Syndrome: An acute, febrile, mucocutaneous condition accompanied by swelling of cervical lymph nodes in infants and young children. The principal symptoms are fever, congestion of the ocular conjunctivae, reddening of the lips and oral cavity, protuberance of tongue papillae, and edema or erythema of the extremities.Proteome: The protein complement of an organism coded for by its genome.Prospective Studies: Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group.Trypanosoma cruzi: The agent of South American trypanosomiasis or CHAGAS DISEASE. Its vertebrate hosts are man and various domestic and wild animals. Insects of several species are vectors.Neoplasm Proteins: Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.

Predominant immunoglobulin A response to phase II antigen of Coxiella burnetii in acute Q fever. (1/1561)

Diagnosis of acute Q fever is usually confirmed by serology, on the basis of anti-phase II antigen immunoglobulin M (IgM) titers of >/=1:50 and IgG titers of >/=1:200. Phase I antibodies, especially IgG and IgA, are predominant in chronic forms of the disease. However, between January 1982 and June 1998, we observed anti-phase II antigen IgA titers of >/=1:200 as the sole or main antibody response in 10 of 1,034 (0.96%) patients with acute Q fever for whom information was available. In order to determine whether specific epidemiological or clinical factors were associated with these serological profiles, we conducted a retrospective case-control study that included completion of a standardized questionnaire, which was given to 40 matched controls who also suffered from acute Q fever. The mean age of patients with elevated phase II IgA titers was significantly higher than that usually observed for patients with acute Q fever (P = 0.026); the patients were also more likely than controls to live in rural areas (P = 0.026) and to have increased levels of transaminase in blood (P = 0.03). Elevated IgA titers are usually associated with chronic Q fever and are directed mainly at phase I antigens. Although the significance of our findings is unexplained, we herein emphasize the fact that IgA antibodies are not specific for chronic forms of Q fever and that they may occasionally be observed in patients with acute disease. Moreover, as such antibody profiles may not be determined by most laboratories, which test only for total antibody titers to phase I and II antigens, the three isotype-specific Ig titers should be determined as the first step in diagnosing Q fever.  (+info)

The STAT3-independent signaling pathway by glycoprotein 130 in hepatic cells. (2/1561)

Interleukin (IL)-6 is a major regulator of hepatic acute-phase plasma protein (APP) genes. The membrane-proximal 133-amino acid cytoplasmic domain of glycoprotein (gp) 130, containing one copy of the Box3 motif, is sufficient to transmit a productive signal to endogenous APP genes in rat hepatoma H-35 cells. In contrast, a mutant gp130 domain lacking the Box3 motif activates Janus kinases to a normal level but fails to activate signal transducer and activator of transcription 3 and to up-regulate a number of APP genes, including thiostatin, fibrinogen, hemopexin, and haptoglobin. However, in the absence of Box3, gp130 still stimulates the expression of alpha2-macroglobulin and synergizes with IL-1 to up-regulate alpha1-acid glycoprotein. The Box3 motif is not required for activation of the SH2-containing protein tyrosine phosphatase 2 or the mitogen-activated protein kinase (MAPK), nor is the immediate induction of egr-1 and junB significantly altered. Surprisingly, gp130 without any functional Box3 stimulates prolonged activation of MAPK, leading to an extended period of up-regulation of egr-1 and to an extracellularly regulated kinase-mediated reduction in the IL-6-stimulated production of thiostatin. IL-6 reduces proliferation of H-35 cells through signaling by the Box3. In addition, cells expressing Box3-deficient gp130 showed distinct morphologic changes upon receptor activation. Taken together, these results indicate that Box3-derived and Box3-independent signals cooperate in the control of hepatic APP genes and that Box3 may be involved in the modulation of MAPK activity in gp130 signaling.  (+info)

Lipopolysaccharide stimulates HepG2 human hepatoma cells in the presence of lipopolysaccharide-binding protein via CD14. (3/1561)

Lipopolysaccharide (LPS)-binding protein (LBP), an opsonin for activation of macrophages by bacterial LPS, is synthesized in hepatocytes and is known to be an acute phase protein. Recently, cytokine-induced production of LBP was reported to increase 10-fold in hepatocytes isolated from LPS-treated rats, compared with those from normal rats. However, the mechanism by which the LPS treatment enhances the effect of cytokines remains to be clarified. In the present study, we examined whether LPS alone or an LPS/LBP complex directly stimulates the hepatocytes, leading to acceleration of the cytokine-induced LBP production. HepG2 cells (a human hepatoma cell line) were shown to express CD14, a glycosylphosphatidylinositol-anchored LPS receptor, by both RT/PCR and flow cytometric analyses. An LPS/LBP complex was an effective stimulator for LBP and CD14 production in HepG2 cells, but stimulation of the cells with either LPS or LBP alone did not significantly accelerate the production of these proteins. The findings were confirmed by semiquantitative RT/PCR analysis of mRNA levels of LBP and CD14 in HepG2 cells after stimulation with LPS alone and an LPS/LBP complex. In addition, two monoclonal antibodies (mAbs) to CD14 (3C10 and MEM-18) inhibited LPS/LBP-induced cellular responses of HepG2 cells. Furthermore, prestimulation of HepG2 cells with LPS/LBP augmented cytokine-induced production and gene expression of LBP and CD14. All these findings suggest that an LPS/LBP complex, but not free LPS, stimulates HepG2 cells via CD14 leading to increased basal and cytokine-induced LBP and CD14 production.  (+info)

Heme and acute inflammation role in vivo of heme in the hepatic expression of positive acute-phase reactants in rats. (4/1561)

Acute-phase protein synthesis in the liver during inflammation is regulated via cytokines and glucocorticoids. Using quantitative reverse transcription (RT)-PCR analysis and immunoassay, we explored, in the rat, the response of the acute-phase protein, alpha-2 macroglobulin (A2M), after systemic inflammation induced by lipopolysaccharide (LPS) or localized inflammation induced by turpentine oil (TO). The results indicate that synthesis of A2M is higher following TO-induced inflammation than LPS-induced inflammation and is not correlated with interleukin (IL)-6 or glucocorticoid levels. We studied the putative role of heme in this differential A2M expression following localized vs. systemic inflammation; addition of heme during LPS-induced inflammation can boost the expression of A2M, whereas blocking heme synthesis (by succinyl acetone) or enhancing its consumption in parallel biosynthetic pathways (cytochrome P450 induction by phenobarbital) decreases A2M expression. This decrease was abolished by exogenous heme supplementation. Finally, we demonstrate that heme supplementation is also able to increase the A2M response in female rats to a level similar to that in male rats providing a new insight into the puzzling sexual dimorphism observed previously during localized inflammation. We propose that heme should be considered a new regulatory element in controlling liver A2M expression during inflammation.  (+info)

Endotoxin interactions with lipopolysaccharide-responsive cells. (5/1561)

Recent work has identified two proteins that work together to enable many cell types to respond to endotoxin. These two proteins, lipopolysaccharide (LPS) binding protein (LBP) and CD14, also participate in cellular internalization of endotoxin, which may occur independently of cellular activation. Current work with antibodies to LBP and CD14 as well as "knockout" mice in the context of LPS-initiated endotoxic shock suggests that inhibition of this pathway could be therapeutically useful. These observations point to the need to identify new molecules that mediate LPS-initiated transmembrane signaling and internalization of LPS-protein complexes.  (+info)

Lipopolysaccharide-coated erythrocytes activate human neutrophils via CD14 while subsequent binding is through CD11b/CD18. (6/1561)

Interaction of LPS with monocytes and neutrophils is known to occur via CD14 and is strongly enhanced by LPS-binding protein (LBP). Integrins as well as CD14 play a role in the interaction of erythrocytes (E) coated with LPS or whole Gram-negative bacteria with phagocytes. We reasoned that the density of LPS on a particle is an important determinant in these interactions. Therefore, E were coated with different concentrations of LPS (ELPS). The binding of these ELPS to neutrophils was evaluated by flow cytometry. Simultaneously, we measured fMLP receptor expression to evaluate neutrophil activation. ELPS only bound to neutrophils in the presence of LBP. Blocking CD14 inhibited both activation and binding, whereas blocking complement (C) receptor 3 (CR3) inhibited binding but not activation. TNF activation restored ELPS binding in CD14-blocked cells but not in cells in which CR3 was blocked. Salmonella minnesota did bind to neutrophils independent of CR3 or CD14. The addition of LBP enhanced binding twofold, and this surplus was dependent upon CD14 but not on CR3. We conclude that ELPS interact with neutrophils via CD14, initially giving rise to cell activation; subsequently, binding is solely mediated by activated CR3.  (+info)

Membrane-anchored forms of lipopolysaccharide (LPS)-binding protein do not mediate cellular responses to LPS independently of CD14. (7/1561)

Inflammatory responses of myeloid cells to LPS are mediated through CD14, a glycosylphosphatidylinositol-anchored receptor that binds LPS. Since CD14 does not traverse the plasma membrane and alternatively anchored forms of CD14 still enable LPS-induced cellular activation, the precise role of CD14 in mediating these responses remains unknown. To address this, we created a transmembrane and a glycosylphosphatidylinositol-anchored form of LPS-binding protein (LBP), a component of serum that binds and transfers LPS to other molecules. Stably transfected Chinese hamster ovary (CHO) fibroblast and U373 astrocytoma cell lines expressing membrane-anchored LBP (mLBP), as well as separate CHO and U373 cell lines expressing membrane CD14 (mCD14), were subsequently generated. Under serum-free conditions, CHO and U373 cells expressing mCD14 responded to as little as 0.1 ng/ml of LPS, as measured by NF-kappaB activation as well as ICAM and IL-6 production. Conversely, the vector control and mLBP-expressing cell lines did not respond under serum-free conditions even in the presence of more than 100 ng/ml of LPS. All the cell lines exhibited responses to less than 1 ng/ml of LPS in the presence of the soluble form of CD14, demonstrating that they are still capable of LPS-induced activation. Taken together, these results demonstrate that mLBP, a protein that brings LPS to the cell surface, does not mediate cellular responses to LPS independently of CD14. These findings suggest that CD14 performs a more specific role in mediating responses to LPS than that of simply bringing LPS to the cell surface.  (+info)

Blood concentrations of pancreatitis associated protein in neonates: relevance to neonatal screening for cystic fibrosis. (8/1561)

AIM: To determine whether pancreatitis associated protein (PAP) is a marker for cystic fibrosis which could be used in neonatal screening for the disease. METHODS: PAP was assayed on screening cards from 202,807 neonates. Babies with PAP > or = 15 ng/ml, or > or = 11.5 ng/ml and immunoreactive trypsinogen (IRT) > or = 700 ng/ml were recalled for clinical examination, sweat testing, and cystic fibrosis transmembrane regulator (CFTR) gene analysis. RESULTS: Median PAP value was 2.8 ng/ml. Forty four cases of cystic fibrosis were recorded. Recalled neonates (n = 398) included only 11 carriers. A receiver operating characteristic curve analysis showed that PAP above 8.0 ng/ml would select 0.76% of babies, including all those with cystic fibrosis, except for one with meconium ileus and two with mild CFTR mutations. Screening 27,146 babies with both PAP and IRT showed that only 0.12% had PAP > 8.0 ng/ml and IRT > 700 ng/ml, including all cases of cystic fibrosis. CONCLUSION: PAP is increased in most neonates with cystic fibrosis and could be used for CF screening. Its combination with IRT looks promising.  (+info)

Acute-phase proteins (APPs) are a class of proteins whose plasma concentrations increase (positive acute-phase proteins) or decrease (negative acute-phase proteins) in response to inflammation. This response is called the acute-phase reaction (also called acute-phase response). The terms acute-phase protein and acute-phase reactant (APR) are often used synonymously, although some APRs are (strictly speaking) polypeptides rather than proteins. In response to injury, local inflammatory cells (neutrophil granulocytes and macrophages) secrete a number of cytokines into the bloodstream, most notable of which are the interleukins IL1, IL6 and IL8, and TNFα. The liver responds by producing a large number of acute-phase reactants. At the same time, the production of a number of other proteins is reduced; these proteins are, therefore, referred to as "negative" acute-phase reactants. Increased acute-phase proteins from the liver may also contribute to the promotion of sepsis. Positive acute-phase ...
Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene.[5][6] LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4.[7] The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma ...
The acute phase protein (APP) response was evaluated after prolonged transportation of pigs under commercial conditions. Elevated serum APP concentrations were observed in two groups of boars immediately after their arrival at a destination farm compared with within-animal control samples obtained one month later. The effect was more pronounced in the first group of pigs conveyed under average transport conditions (Transport 1, 24 h), although the second group was transported for a longer time period (Transport 2, 48 h) but in superior transport conditions. In a second trial, pigs were sampled before transport, on arrival at an abattoir (following 12 h transport), and at the slaughter-line (after 6 h lairage). Significant increases in major acute phase protein (Pig-MAP), haptoglobin, serum amyloid A, C-reactive protein, and a decrease in apolipoprotein A-I, were observed at slaughter. The results demonstrate that shipment of pigs by road can result in an APP response that is probably related to ...
Background: Inhibition of intracellular signal transduction is considered to be an interesting target for treatment in inflammation. p38 MAPK inhibitors, especially, have been developed and are now in phase II clinical trials for rheumatoid arthritis (RA).. Objective: To investigate the influence of p38 MAPK inhibition on acute phase protein (APP) production, which is dependent on both JAK/STAT and p38 MAPK pathways.. Methods: The effects of p38 MAPK inhibition on APP production and mRNA expression in four human hepatoma cell lines was investigated, after stimulation with interleukin (IL)6 and/or IL1β or tumour necrosis factor α.. Results: Two out of four cell lines produced C reactive protein (CRP), especially after combined IL6 and IL1β stimulation. CRP production was significantly inhibited by the p38 MAPK specific inhibitor RWJ 67657 at 1 μmol/l, which is pharmacologically relevant. Fibrinogen production was also inhibited at 1 μmol/l in all cell lines. Serum amyloid A (SAA) was ...
LBP [LPS (lipopolysaccharide)-binding protein] was discovered approximately 25 years ago. Since then, substantial progress has been made towards our understanding of its function in health and disease. Furthermore, the discovery of a large protein family sharing functional and structural attributes has helped in our knowledge. Still, key questions are unresolved, and here an overview on the old and new findings on LBP is given. LBP is an acute-phase protein of the liver, but is also synthesized in other cells of the organism. While LBP is named after the ability to bind to LPS of Gram-negative bacteria, it also can recognize other bacterial compounds, such as lipopeptides. It has been shown that LBP is needed to combat infections; however, the main mechanism of action is still not clear. New findings on natural genetic variations of LBP leading to functional consequences may help in further elucidating the mechanism of LBP and its role in innate immunity and disease. ...
Acute phase protein levels increase in blood and other bodily fluids as a result of inflammation, tissue injury and disease. They are used as biomarkers of disease in humans, companion animals, livestock and species used in preclinical research. When selecting an acute phase biomarker it is important to appreciate that a good biomarker in one species is not necessarily useful in other species. For example, C-reactive protein (CRP) can increase as much as 1000-fold in humans and dogs but it increases by only 50% in rats. Please contact us if you would like advice regarding biomarker selection. We currently manufacture ELISA kits for measurement of acute phase proteins including CRP, haptoglobin, hemopexin, alpha-1 acid glycoprotein, clusterin, alpha-2-macroglobulin, serum amyloid A (SAA) and serum amyloid P (SAP) in species including but not limited to mouse, rat, rabbit, cat, dog, pig, cow, chicken, and monkey.. Data that illustrate the use of our kits to measure acute phase proteins in mouse ...
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F. Carrouel, M. J Staquet, J. F Keller, C. Baudouin, P. Msika, F. Bleicher, B. Alliot-Licht, and J. C Farges (2013) J Endod, 39(8):1008-14.. ...
In this study, we provide evidence based on both functional assays and structural modeling that is consistent with EsLBP1 functioning as an LBP-like protein. Most notably, EsLBP1 binds Gram-negative bacterial LOS and LPS with nanomolar or higher avidity under in vitro conditions, i.e., when LPS/LOS is presented as part of supramolecular assemblies containing LPS-rich lipid-water interfaces, as in aggregates of purified LPS/LOS. eslbp1 gene expression is regulated by exposure to the peptidoglycan monomer TCT, which synergizes with LPS in the triggering of V. fischeri-induced morphogenesis of the host symbiotic tissues. The gene is expressed and the protein produced across the organs epithelia-from the point where V. fischeri initially gathers, along the path of its migration, to where it takes up permanent residence in the crypts. The protein is also abundant along the apical surfaces of other epithelial tissues, where colonization by bacteria does not occur.. Although EsLBP1 has only ~25% ...
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... Acute-phase proteins are a class of proteins whose plasma concentrations increase (positive acute phase proteins) or decrease (negative
The diet transition phase is thought to be the highest risk period for development of low ruminal pH, while pathology associated with low reticulo-ruminal pH (RRpH) induced ruminal acidosis (RA) is often found at slaughter, months after the diet transition. Two experiments were conducted to 1) determine the risk of low RRpH during the transition phase and 2) explore the association of rumen fermentation and acute phase protein response during finishing with pathology identified post mortem. In experiment 1, RRpH was measured in 32 mixed breed steers (n = 16) and heifers (n = 16) housed in commercial feedlot pens with 227 ±13 and 249 ± 6 hd/pen cohort steers and heifers, respectively. Cattle were transitioned from a diet containing 46.5% forage and 53.5% concentrate to a diet containing 9.5% forage and 90.5% concentrate dry matter (DM) basis) over 40 d. In addition, wheat replaced barley as the grain source during the dietary transition. Both mean and minimum RRpH decreased as the proportion of ...
Assessment of serum concentration of lipopolysaccharide (LPS)-binding protein (LBP) has been suggested as a useful biomarker to indicate activation of innate immune responses to microbial products. We investigated LBP concentrations and associations with demographics, lifestyle factors, and common metabolic abnormalities in adults. We also examined if LBP concentrations were associated with common polymorphisms in genes coding for LBP (rs2232618), CD14 (rs2569190), and TLR4 (rs4986790), the molecules responsible for the innate immune response to LPS, or serum levels of soluble CD14 (sCD14) and proinflammatory cytokines ...
Correction: 3LPS-binding protein and its interactions with P. gingivalis LPS modulate pro-inflammatory response and Toll-like receptor signaling in human oral keratinocytes. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Twenty patients with invasive cervical cancer of the squamous cell type were treated by radiotherapy and/or radical hysterectomy. During a follow-up period of approximately 2 yr in 10 of these patients a recurrence was established. Serial determinations of three acute phase reactants (α1-acid glycoprotein, haptoglobin and C-reactive protein) and of ... read more three complement components (C4, C3-proactivator and C9) were performed before and after therapy and either at the time of recurrence (recurrence group), or at the end of the follow-up period (non-recurrence group). Sequential analysis of the serum levels of all the parameters tested showed a significant increase at the time of recurrence. The most conclusive results were obtained with haptoglobin and the C9-component of complement. show less ...
The aim of this study was to investigate the effects of a housing period following long distance transport on haptoglobin (Hp), C-reactive protein (CRP) and pig major acute phase protein (pig-MAP) in swine. After transportation, 80 gilts were allotted to group A, B, C, or D. Blood samples were collected on arrival and 28 days later; additional samples were collected from Group C on day 14, and fro ...
Results : Body weight, % body fat, fasting glucose, total cholesterol, and NEFA were increased with WD and wheat had no effect on these metabolic parameters. Serum C reactive protein and lipopolysaccharide binding protein were not changed by WD or wheat. WD decreased the SCFA, acetic acid, but adding Gallagher wheat to WD restored levels to control (PWD*Wheat, 0.05). No other SCFA were altered. Histological evaluation revealed reduced villi height (P, 0.05) and area (P, 0.05) in the jejunum with WD and wheat did not alter this response. Within the ileum, Gallagher increased villi area (P, 0.01) relative to control, but no other changes were noted. No effects of WD or wheat on villous atrophy or lymphocyte infiltration within the jejunum, ileum or colon were observed. Overall, gene expression of tight junction proteins was unaffected by WD or wheat, except for a reduction in junction adhesion molecule-3 (Jam3) by WD (P, 0.05). Within the ilial lamina propria, WD increased interferon-γ (IFNg) (P, ...
IL-6 is produced by APCs such as macrophages, dendritic cells, and B cells, but also by nonhematopoietic cells (e.g., epithelial and endothelial cells, astrocytes, and fibroblasts) in response to external stimuli such as TNF-α, IL-1β, platelet-derived growth factor, or bacterial and fungal components. It binds to a receptor complex consisting of the specific IL-6Rα and the gp130 signal transducing unit that activates the transcription factor STAT3, among other signaling molecules. IL-6 is a pleiotropic cytokine that plays an important role in acute-phase protein synthesis, bone metabolism, central nervous system function, growth and drug response of tumors, and the immune response (for reviews see references 1, 2). Regarding its function in the immune system, IL-6 is now thought of as an important link between innate and adaptive immunity, mediating several aspects of B and T cell responses (3).. The role of IL-6 in CD4+ T cell function is multifaceted. IL-6 influences T cell effector ...
The proteasome as a lipopolysaccharide-binding protein in macrophages: differential effects of proteasome inhibition on lipopolysaccharide-induced signaling events ...
The host inflammatory response against infections is characterized by the release of pro-inflammatory cytokines and acute-phase proteins, driving both innate and adaptive arms of the immune response. Distinct patterns of circulating cytokines and acute-phase responses have proven indispensable for guiding the diagnosis and management of infectious diseases. This review discusses the profiles of acute-phase proteins and circulating cytokines encountered in viral and bacterial infections. We also propose a model in which the inflammatory response to viral (IL-18/ferritin) and bacterial (IL-6/CRP) infections presents with specific plasma patterns of immune biomarkers ...
Diabetes mellitus is one of public health problem in world. Type 2 diabetes millitus (T2DM) which resistance cell to insulin. more inflammation occur in Patients with T2DM because of elevation of inflammatory marker like C3, C4 & hsCRP. This study was aimed to detect the level of acute phase reactant proteins (C3 , C4 and hsCRP) in T2DM patients. Blood samples were collected from 60 patients with T2DM and control group was included 30 healthy donors. C3, C4 & hsCRP levels were estimated for all samples. Samples were grouped according to complications of patients. The results showed that there was a significant increase in C3 level for patients with T2DM (199.0 mg/dl) compared to control group (135.4 mg /dl) while C4 level for T2DM (27.25 mg/dl) showed no significant change compared to control group( 30.04mg/dl). C3 level of patients under heart disease (199.6 mg/dl ), patients under thyroid disease (230.4 mg/dl) and patients without any disease (188.1 mg/dl) recorded significant elevation
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Interleukin 1 receptor antagonist (IL-1Ra) is an acute-phase protein.: Interleukin 1 receptor antagonist (IL-1Ra) levels are elevated in the blood of patients w
Anogen is a Canadian biopharmaceutical company, who has been producing antibody products for research, diagnostic and therapeutic applications.
CD14 Antigens: Glycolipid-anchored membrane glycoproteins expressed on cells of the myelomonocyte lineage including monocytes, macrophages, and some granulocytes. They function as receptors for the complex of lipopolysaccharide (LPS) and LPS-binding protein.
Objective: We aimed to determine the potential value of maternal serum levels of acute phase reactants in the prediction of preterm delivery in women with ...
Simpson, R. J., Guy, K., Whyte, G. P., Middleton, N., Black, J. R., Ross, J. A., …Florida-James, G. D. (2006). Lymphocyte Phenotype Alterations, Pro-Inflammatory Cytokines and Acute Phase Proteins Following Repeated Bouts of Mountainous Hill-Running: 2278. Medicine and science in sports and exercise. 38(Supplement), S412???S413. doi:10.1249/00005768-200605001-01739. ISSN 0195-9131. ...
Simpson, R. J., Guy, K., Whyte, G. P., Middleton, N., Black, J. R., Ross, J. A., …Florida-James, G. D. (2006). Lymphocyte Phenotype Alterations, Pro-Inflammatory Cytokines and Acute Phase Proteins Following Repeated Bouts of Mountainous Hill-Running: 2278. Medicine and science in sports and exercise. 38(Supplement), S412???S413. doi:10.1249/00005768-200605001-01739. ISSN 0195-9131. ...
Heatmap depicting circulating microbial products, acute phase proteins and inflammatory cytokines in CP Ag+ individuals compared to EN, INF and CP Ag− individ
SPR reveals ColN‐R is responsible for LPS binding. Histidine‐tagged ColN domain combinations (500 nM) were injected for 60 s at a flow rate of 5 μl m
Diagnosis of acute kidney injury (AKI) relies on a late marker, namely serum creatinine (SCr). New biomarkers are considered for early and sensitive detection of CIN. In particular, uNGAL has been used for early detection of AKI in the emergency department, after cardiopulmonary bypass or following CM administration.. This study will be conducted to assess the possible value of urinary Neutrophil Gelatinase-Associated Lipocalin (uNGAL) as an early detector of contrast-induced nephropathy (CIN) in a large sized cohort of patients undergoing percutaneous coronary procedures (PCP) and whether or not uNGAL correlates with the volume of contrast medium (CM) used. ...
TY - JOUR. T1 - Neutrophil Gelatinase-Associated Lipocalin Is Not Associated with Tacrolimus-Induced Acute Kidney Injury in Liver Transplant Patients Who Received Mycophenolate Mofetil with Delayed Introduction of Tacrolimus. AU - Fukuda, Mio. AU - Suetsugu, Kimitaka. AU - Tajima, Soichiro. AU - Katsube, Yurie. AU - Watanabe, Hiroyuki. AU - Harada, Noboru. AU - Yoshizumi, Tomoharu. AU - Egashira, Nobuaki. AU - Mori, Masaki. AU - Masuda, Satohiro. PY - 2019/6/25. Y1 - 2019/6/25. N2 - Tacrolimus is widely used as an immunosuppressant in liver transplantation, and tacrolimus-induced acute kidney injury (AKI) is a serious complication. The urinary neutrophil gelatinase-associated lipocalin (NGAL) level has been linked to tacrolimus-induced AKI in patients starting tacrolimus treatment the morning after liver transplantation. Here we tested this association using a different immunosuppression protocol: Mycophenolate mofetil administration beginning on Postoperative Day 1 and tacrolimus administration ...
Different molecular forms of urinary neutrophil gelatinase-associated lipocalin (NGAL) have recently been discovered. We aimed to explore the nature, source and discriminatory value of urinary NGAL in
García, T., Betbesé, A., García, M. et al. Erratum to: Urinary neutrophil gelatinase-associated lipocalin as an early predictor of acute kidney injury in cardiac surgery patients. Crit Care 17, P546 (2013). https://doi.org/10.1186/cc12604. Download ...
Acute kidney injury (AKI) is a devastating potential consequence of renal ischaemia and reperfusion (I-R) subsequent to severe intra-operative hypotension and fluid resuscitation. Acute tubular epithelial damage is a common early histological abnormality in this syndrome. The high mortality rate associated with AKI in dogs is attributed in part to the limitations of current diagnostic techniques that can only detect AKI in the late stages when damage is irreversible. Early detection of renal tubular injury could improve outcome and might be possible by measuring urinary neutrophil gelatinase-associated lipocalin concentration (uNGAL) in at-risk dogs.. The objectives of this study were to establish a clinically relevant canine model of renal I-R injury, and use this model to determine changes in uNGAL within three hours of initiation of injury.. A pilot study was performed to establish the severity and duration of hypotension caused by haemorrhage, and duration of reperfusion, that produced ...
Neutrophil gelatinase-associated lipocalin has been used for the diagnosis, prognosis and severity assessment of AKI and has been validated in paediatric populations (where comorbidity is low) and in conditions where the timing of the insult is clear (that is cardiac surgery and so on) [20-24]. Its role in an adult ICU population has not been well validated due to the heterogeneity and uncertainty of the timing of the insult. The pathophysiology and causes of AKI in the ICU could be indigenous [25] and may differ from pre-ICU causes (low-volume state, inotropes, contrast injury and so on). This has led to problems in the design and interpretation of studies in the general adult ICU patient cohort. In this study, we sought to overcome these problems by excluding patients with pre-existing chronic kidney disease (CKD) and/or AKI and by looking at the predictive value of both urinary and plasma NGAL at different time points following admission.. We found the incidence of AKI was 30.4% (n = 59) with ...
TY - JOUR. T1 - Neutrophil gelatinase-associated lipocalin regulates gut microbiota of mice. AU - Mori, Katsuya. AU - Suzuki, Takeshi. AU - Minamishima, Shizuka. AU - Igarashi, Toru. AU - Inoue, Kei. AU - Nishimura, Daisuke. AU - Seki, Hiroyuki. AU - Yamada, Takashige. AU - Kosugi, Shizuko. AU - Katori, Nobuyuki. AU - Hashiguchi, Saori. AU - Morisaki, Hiroshi. PY - 2016/1/1. Y1 - 2016/1/1. N2 - Background and Aim: Because neutrophil gelatinase-associated lipocalin (NGAL) is known to provide significant bacteriostatic effects during infectious conditions, we tested the hypothesis that this protein is up-regulated and secreted into the intraluminal cavity of the gut under critically ill conditions and is thus responsible for the regulation of bacterial overgrowth. Methods: With our institutional approval, male C57BL/6J mouse (6-7weeks) were enrolled and applied for lipopolysaccharide or peritonitis model compared with naïve control. We assessed NGAL protein concentrations in intestinal lumen and ...
Neutrophil gelatinase-associated lipocalin (NGAL) is a protein expressed by kidney tubular cells in response to ischemia, but may also be an early indicator of immunological rejection, calcineurin inhibitor toxicity, obstructive nephropathy, subclinical tubulitis or infection. Although there is currently no evidence to support the routine serial measurement of blood or urinary NGAL to detect subclinical acute tubular injury, NGAL has the potential to provide useful information to those that care for kidney transplant recipients (KTRs). First, high urinary or serum NGAL concentrations shortly after transplantation are a predictor of delayed graft function and are associated with reduced graft function at one year. Secondly, among KTRs with previously stable graft function who then suffer acute graft dysfunction, a high urinary NGAL predicts graft loss at one year. If further refined, diagnostic tests based on NGAL levels may provide future useful clinical tools.
Neutrophil gelatinase-associated lipocalin (NGAL) is a small 25-kDa protein released from kidney tubular cells after harmful stimuli. It represents one of the most promising future biomarkers in the diagnostic field of acute kidney injury (AKI), as the increase in NGAL levels is a good predictor of a brief-term onset of AKI, notably anticipating the resulting increase in serum creatinine. However, recent studies also suggest a possible role for NGAL in chronic kidney disease (CKD). For this reason we evaluated serum (sNGAL) and urinary NGAL (uNGAL) in a cohort of CKD patients in order to verify the relationship with the severity of renal impairment. In CKD patients sNGAL, uNGAL and the fractional excretion of this protein were notably increased as compared to controls. Furthermore both sNGAL and uNGAL were correlated with serum creatinine and, inversely, with residual glomerular filtration rate (GFR): this last relationship was found to be even closer than that found between GFR and serum ...
Lipocalin-2, human recombinant protein, Neutrophil gelatinase-associated lipocalin, NGAL, p25, 25 kDa alpha-2-microglobulin-related subunit validated in (PBV10492r-10), Abgent
Evaluation of Neutrophil Gelatinase-Associated Lipocalin and Cystatin C in Early Diagnosis of Chronic Kidney Disease in the Absence of the Gold Standard
Nephropathia epidemica (NE) is a mild form of hemorrhagic fever with renal syndrome (HFRS) that is caused by the Puumala virus. Periodic outbreaks have been described in endemic areas, with a substantial number of previously healthy individuals developing acute kidney injury (AKI). There is a considerable diversity in the clinical course of the disease, and few patients require renal replacement therapy. We tested whether urinary neutrophil gelatinase associated lipocalin (uNGAL), urine albumin/creatinine ratio (uACR), urine protein/creatinine ratio (uPCR), urine dipstick protein, C-reactive protein, procalcitonin, leukocyte and platelet count, determined on admission to the hospital, can predict the severity of AKI. Sixty-one patients were analyzed during admission in the emergency department. The variables most strongly associated with peak plasma creatinine concentration were uNGAL (β = 0.70, p |0.0001), uPCR (β = 0.64, p = 0.001), uACR (β = 0.61, p = 0.002), and dipstick proteinuria (β = 0.34, p
Abstract Elderly is the main age group affected by acute kidney injury (AKI). There are no studies that investigated the predictive properties of urinary (u) NGAL as an AKI marker in septic elderly population. This study aimed to evaluate the efficacy of uNGAL as predictor of AKI diagnosis and prognosis in elderly septic patients admitted to ICUs. We prospectively studied elderly patients with sepsis admitted to ICUs from October 2014 to November 2015. Assessment of renal function was performed daily by serum creatinine and urine output. The level of uNGAL was performed within the first 48 hours of the diagnosis of sepsis (NGAL1) and between 48 and 96 hours (NGAL2). The results were presented using descriptive statistics and area under the receiver operating characteristic curve (AUC-ROC) and p value was 5%. Seventy-five patients were included, 47 (62.7%) developed AKI. At logistic regression, chronic kidney disease and low mean blood pressure at admission were identified as factors associated ...
One-hundred and eighty-one patients (66.1%) were men; mean age was 68.2 ± 12.2 years. Valve replacement was performed in 123, coronary artery bypass graft (CABG) in 81, valve surgery + CABG in 48, cardiac transplant in five, aorta aneurism surgery in nine, and other procedures in eight patients. ICU and hospital stays were 6.7 ± 8.1 and 15.7 ± 13.9 days, respectively. Renal replacement therapy (RRT) was required in 16 patients (5.8%) within 48 hours of ICU stay and in 28 patients (10.2%) within 43weeks. Mortality at 28 days was 2.9%. Eighty-six patients (31.4%) were diagnosed with AKI within 48 hours of surgery. Area under the ROC curve of POST uNGAL for AKI diagnosis was 0.72 (0.66 to 0.79) (P 0.0001) at an optimal cutoff value of 1803 μg/l, with 78.7% specificity, 64% sensitivity and 74.1% accuracy. uNGAL advanced diagnosis of AKI in 44 patients (51.2%), whereas diagnosis was achieved at the same time as AKI criteria in 11 patients; AKI criteria outperformed uNGAL in only 36% of cases. ...
AKI occurs frequently in the hospital setting and affects morbidity, mortality, and resource use, regardless of country, etiology, setting, or definition. In current practice, AKI is identified by a large, persistent increase in serum creatinine levels. However, physicians want to diagnose AKI as early as possible, and the serum creatinine increase happens days after AKI occurs. Early changes in serum creatinine are not particularly ...
Semantic Scholar extracted view of Neutrophil gelatinase-associated lipocalin as an early indicator for postoperative renal failure by CD Van der Marel et al.
敗血症(Sepsis)是造成急性腎損傷(Acute kidney injury, AKI)的主因,而AKI又為敗血症患者死因之一,研究認為NGAL(Neutrophil gelatinase-associated lipocalin)可作為預測AKI的生物標誌,但因NGAL又為感染及全身性發炎的生物標誌,可能受多重因素干擾。目前AKI患者可依RIFLE(Risk、Injury、Failure、Loss of kidney function及End-stage kidney disease)分級,若腎臟代替療法(Renal replacement therapy)於RIFLE-Failure(F)期之前進行,可提升治療結果,但AKI患者接受腎臟代替療法時機仍未有共識,因此發表於《Journal of the Formosan Medical Association》的最新文章評估血漿中NGAL及細胞激素是否可作為預測RIFLE-F及啟動腎臟代替療法的生物標誌。試驗中,30位健康受試者及85位患者接受採血,分析肌酸酐(creatinine, ...
A lateral flow device comprised of a strip of porous nitrocellulose is coated near its distal end with a capture antibody against NGAL applied as a transverse band. A further transverse band of antibody against antibodies of the species from which the detection antibody is derived is placed distally to the capture antibody band and serves as a control of strip function. The proximal end of the strip contains the detection antibody against NGAL adsorbed or linked to labeled polystyrene particles or particles of dye complex. When an aliquot of the centrifuged urine sample is applied to the proximal end of the strip, the labeled particles attached to detection antibody travel along the strip by capillary attraction. When reaching the band of capture antibody, only those particles which have bound NGAL will be retained, giving rise to a detectable band. Particles reaching the control band of antibody against the detection antibody will produce a detectable band whether or not any NGAL has been ...
Nøytrofil gelatinase-assosiert lipocalin i inflammatorisk tarmsykdom Inflammatorisk tarmsykdom (IBD) er kroniske betennelsestilstander i mage-tarmsystemet. De to hovedformene er Crohns sykdom (CD) og ulcerøs kolitt (UC). Årsaken til sykdommene er ukjent. Sykdomsmekanismene er komplekse og involverer blant annet genetiske faktorer, komponenter av det medfødte og ervervede immunforsvaret og tarmens mikrobeflora. Eksisterende behandling er utilfredsstillende. Mye forskning har blitt utført for å forstå sykdomsmekanismene bedre, for å finne bedre diagnostiske verktøy og bedret behandling. Vår forskningsgruppe har tidligere utført analyse av genregulering i tykktarmsslimhinne der det ble funnet at genet LCN2 var blant de mest oppregulerte genene ved aktiv betennelse sammenlignet med kontroller. Proteinet som kodes av LCN2, nøytrofil gelatinase-assosiert lipocalin (NGAL), var høyt uttrykt i en klasse av hvite blodlegemer og i betente overflateceller (epitel). NGAL er kjent som et ...
Looking for online definition of Acute phase reactant in the Medical Dictionary? Acute phase reactant explanation free. What is Acute phase reactant? Meaning of Acute phase reactant medical term. What does Acute phase reactant mean?
... is an acute phase protein and is therefore seen to decrease in inflammation, cancers, and certain diseases. ... permanent dead link] Jain S, Gautam V, Naseem S (Jan 2011). "Acute-phase proteins: As diagnostic tool". Journal of Pharmacy & ... "Reference distributions for the negative acute-phase serum proteins, albumin, transferrin and transthyretin: a practical, ... "Transferrin is an insulin-like growth factor-binding protein-3 binding protein". The Journal of Clinical Endocrinology and ...
... (ORM) or alpha-1-acid glycoprotein (α1AGp,[1] AGP or AAG) is an acute phase (acute phase protein) plasma alpha- ... The effect of these changes on drug protein binding and drug delivery, however, appear to be minimal.[4] AGP shows a complex ... It is synthesized primarily in hepatocytes and has a normal plasma concentration between 0.6-1.2 mg/mL (1-3% plasma protein).[2 ...
Blood proteins. *Acute phase proteins. Hidden categories: *CS1 German-language sources (de) ... Fibrin (also called Factor Ia) is a fibrous, non-globular protein involved in the clotting of blood. It is formed by the action ... Fibrin forms long strands of tough insoluble protein that are bound to the platelets. Factor XIII completes the cross-linking ... Fibrin: Molecule of the Month, by David Goodsell, RCSB Protein Data Bank ...
Acute phase proteins are markers of inflammation. Autoantibodies are usually absent or very low, so instead of being given in ... "C-reactive protein". GPnotebook. 2730 Serum C-Reactive Protein values in Diabetics with Periodontal Disease A.R. Choudhury, and ... Derived from mass values using molar mass of 314.46 g/mol Bhattacharya Sudhindra Mohan (July/August 2005) Mid-luteal phase ... Included here are also related binding proteins, like ferritin and transferrin for iron, and ceruloplasmin for copper. If ...
OSM can regulate the expression of acute phase proteins. OSM regulates the expression of various protease and protease ... Of the proteins recruited to type I cytokine receptors STAT proteins remain the best studied. Homodimerisation of gp130 has ... "Differential activation of acute phase response factor/STAT3 and STAT1 via the cytoplasmic domain of the interleukin 6 signal ... effect on the acute phase reaction". Z Ernahrungswiss. 37 Suppl 1: 43-9. PMID 9558728. Schieven GL, Kallestad JC, Brown TJ, ...
... acute phase proteins / reactants such as C-reactive protein; (3) adipokines of the alternative complement system; (4) ... such as C-Reactive protein, interleukin-6, and tumor necrosis alpha) with weight gain Abnormalities in lipoprotein particle ...
Many acute-phase proteins of inflammation are involved in the coagulation system. Also increased levels of lactoferrin and ... This leads to antiviral protein production, such as protein kinase R, which inhibits viral protein synthesis, or the 2′,5′- ... "Resistance" (R) proteins, encoded by R genes, are widely present in plants and detect pathogens. These proteins contain domains ... The cascade is composed of many plasma proteins, synthesized in the liver, primarily by hepatocytes. The proteins work together ...
Many acute-phase proteins of inflammation are involved in the coagulation system. In addition, pathogenic bacteria may secrete ... Thrombomodulin binds these proteins in such a way that it activates Protein C. The activated form, along with protein S and a ... as well as Protein S, Protein C and Protein Z. In adding the gamma-carboxyl group to glutamate residues on the immature ... Protein C is activated in a sequence that starts with Protein C and thrombin binding to a cell surface protein thrombomodulin. ...
Disulfide groups stabilize the tertiary structures of proteins. Transferrins, are iron binding proteins and acute phase ... Egg white albumen is composed of multiple proteins and ovotransferrin is the most heat reliable protein of them all. It has a ... Consequently, structurally this protein differs from its serum counterpart because of its glycosylation pattern. These proteins ... ovotransferrin has been found to produce heat shock proteins. When these heat shock proteins are induced in the skin, they ...
This test utilises the acute phase proteins (C-Reactive Protein and Haptoglobin). In combination with basic clinical symptoms, ... Serum thymadine kinase 1 and C-reactive protein as biomarkers for screening clinically healthy dogs for occult disease. K A ... Hypercalcemia in these cases is caused by secretion of parathyroid hormone-related protein. Multicentric lymphoma presents as ...
This gene encodes a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is ... "Acute phase protein alpha 1-acid glycoprotein interacts with plasminogen activator inhibitor type 1 and stabilizes its ... sequence homology with other human acute phase protein genes". Nucleic Acids Res. 13 (11): 3941-52. doi:10.1093/nar/13.11.3941 ... classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however, it may be ...
As ferritin is also an acute-phase reactant, it is often elevated in the course of disease. A normal C-reactive protein can be ... Ferritin is a globular protein complex consisting of 24 protein subunits forming a nanocage with multiple metal-protein ... this implies that it is an acute phase protein. Mitochondrial ferritin has many roles pertaining to molecular function. It ... Ong DS, Wang L, Zhu Y, Ho B, Ding JL (2005). "The response of ferritin to LPS and acute phase of Pseudomonas infection". ...
1993). "Ciliary neurotrophic factor induces acute-phase protein expression in hepatocytes". FEBS Lett. 314 (3): 280-4. doi: ... "Ciliary neurotrophic factor induces acute-phase protein expression in hepatocytes". FEBS Lett. 314 (3): 280-4. doi:10.1016/0014 ... Ciliary neurotrophic factor is a protein that in humans is encoded by the CNTF gene. The protein encoded by this gene is a ... Phase III clinical trials for the drug against obesity were conducted in 2003 by Axokine's maker, Regeneron, demonstrating a ...
Schooltink H, Stoyan T, Roeb E, Heinrich PC, Rose-John S (Dec 1992). "Ciliary neurotrophic factor induces acute-phase protein ... Schooltink H, Stoyan T, Roeb E, Heinrich PC, Rose-John S (1992). "Ciliary neurotrophic factor induces acute-phase protein ... PTH-related protein receptor gene". Endocrinology. 140 (2): 925-32. doi:10.1210/en.140.2.925. PMID 9927325. IL6R protein, human ... The IL6 receptor is a protein complex consisting of an IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer ...
"Relationship of TSG-14 protein to the pentraxin family of major acute phase proteins". J. Immunol. 153 (8): 3700-7. PMID ... PTX3 behaves as an acute phase response protein, as the blood levels of PTX3, low in normal conditions (about 25 ng/mL in the ... Pentraxin-related protein PTX3 also known as TNF-inducible gene 14 protein (TSG-14) is a protein that in humans is encoded by ... is a novel member of the pentaxin family of acute phase proteins". J. Immunol. 150 (5): 1804-12. PMID 7679696. Alles VV, ...
1990). "Serum amyloid A, an acute phase protein, inhibits platelet activation". J. Lab. Clin. Med. 116 (2): 180-6. PMID 1697614 ... Ancsin JB, Kisilevsky R (1997). "Characterization of high affinity binding between laminin and the acute-phase protein, serum ... an acute-phase serum amyloid A protein gene (SAA2)". Genomics. 16 (2): 447-54. doi:10.1006/geno.1993.1209. PMID 7686132. " ... "Entrez Gene: SAA2 Serum amyloid A2". Betts JC, Edbrooke MR, Thakker RV, Woo P (1991). "The human acute-phase serum amyloid A ...
Since those are Acute-phase proteins, a positive Rivalta's test may be suggestive of inflammation. To perform this test, a ... Using a pH 4.0 acetic acid solution, 8 types of proteins were identified in Rivalta reaction-positive turbid precipitates: C- ... Not only the high protein content, but high concentrations of fibrinogen and inflammatory mediators lead to a positive reaction ... reactive protein (CRP), Alpha 1-antitrypsin (alpha1-AT), Orosomucoid ((Alpha-1-acid glycoprotein or AGP)), haptoglobin (Hp), ...
Acute-phase serum amyloid A proteins (A-SAAs) are secreted during the acute phase of inflammation. These proteins have several ... Serum amyloid A (SAA) is also an acute phase marker that responds rapidly. Similar to CRP, levels of acute-phase SAA increase ... acute phase SAAs). These proteins are produced predominantly by the liver. The conservation of these proteins throughout ... an acute-phase serum amyloid A protein gene (SAA2)". Genomics. 16 (2): 447-54. doi:10.1006/geno.1993.1209. PMID 7686132. de ...
Liu Z, Ukomadu C (2008). "Fibrinogen-like protein 1, a hepatocyte derived protein is an acute phase reactant". Biochem. Biophys ... Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FLG-1 is encoded by the ... Fibrinogen-like protein 1 is a member of the fibrinogen family of proteins, which also includes fibrinogen, fibrinogen-like ... 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. doi:10.1038 ...
These globular regions of C1q can also bind to bacterial and viral surface proteins, apoptotic cells, and acute phase proteins ... The classical complement pathway can also be activated by apoptotic cells, necrotic cells, and acute phase proteins. The ... Following activation, a series of proteins are recruited to generate C3 convertase (C4bC2b), which cleaves the C3 protein. The ... In addition, the C5 convertase initiates the terminal phase of the complement system, leading to the assembly of the membrane ...
... (SAA1) is a protein that in humans is encoded by the SAA1 gene. SAA1 is a major acute-phase protein mainly ... Zimlichman S, Danon A, Nathan I, Mozes G, Shainkin-Kestenbaum R (Aug 1990). "Serum amyloid A, an acute phase protein, inhibits ... "Entrez Gene: SAA1 serum amyloid A1". Gabay C, Kushner I (Feb 1999). "Acute-phase proteins and other systemic responses to ... Malle E, Sodin-Semrl S, Kovacevic A (Jan 2009). "Serum amyloid A: an acute-phase protein involved in tumour pathogenesis". ...
... an adaptor/docking protein, modulates STAT3 activation in acute-phase response through its YXXQ motif". J. Biol. Chem. 278 (13 ... Signal-transducing adaptor protein 1 is a protein that in humans is encoded by the STAP1 gene. The protein encoded by this gene ... The protein is directly phosphorylated by Tec in vitro where it participates in a positive feedback loop, increasing Tec ... A mouse ortholog, stem cell adaptor protein 1, shares 83% identity with its human counterpart. STAP1 has been shown to interact ...
It is theorized that, because of this, haptoglobin has evolved into an acute-phase protein. HP has a protective influence on ... As haptoglobin is indeed an acute-phase protein, any inflammatory process (infection, extreme stress, burns, major crush injury ... The chains originate from a common precursor protein, which is proteolytically cleaved during protein synthesis. Hp exists in ... For this reason, it is often referred to as the suicide protein. Haptoglobin is produced mostly by hepatic cells but also by ...
C-reactive protein is an acute phase protein produced by the liver during an inflammatory reaction. Since C-reactive protein ... Jens Georg Hansen; Henrik Schmidt; Jorn Rosborg; Elisabeth Lund (22 July 1995). "Predicting acute maxillary sinusitis in a ... low plasma protein (due to liver or kidney disease) and congestive heart failure. The basal ESR is slightly higher in females. ... both tests for ESR and CRP were found to be independently associated with a diagnosis of acute maxillary sinusitis so in some ...
2003). "Hepcidin, a putative mediator of anemia of inflammation, is a type II acute-phase protein". Blood. 101 (7): 2461-3. doi ... Hepcidin is a protein that in humans is encoded by the HAMP gene. The product encoded by this gene is involved in the ... 2003). "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and ... encoding a protein homologous to human antimicrobial peptide hepcidin, is overexpressed during iron overload". J Biol Chem. 276 ...
Lomitapide is a microsomal triglyceride transfer protein inhibitor.. *Phytosterols may be found naturally in plants. Similar to ... succinobucol(AGI-1067), a novel antioxidant, failed a phase-III trial.. *Apoprotein-B inhibitor mipomersen (approved by the FDA ... AMDA - The Society for Post-Acute and Long-Term Care Medicine (February 2014), "Ten Things Physicians and Patients Should ... CETP inhibitors (cholesteryl ester transfer protein), 1 candidate is in trials. It is expected that these drugs will mainly ...
Acute Phase Proteins-Regulation and Functions of Acute Phase Proteins. InTech; 2011. ... Neutrophil gelatinase-associated lipocal in protein levels as an acute appendicitis biomarker in children. 2016;5(1):193. ... Glomerular filtration rate and albuminuria for detection and staging of acute and chronic kidney disease in adults: a ... Shang W, Wang ZJCP, Science P. The Update of NGAL in Acute Kidney Injury. 2017;18(12):1211-7. ...
Secondly, among KTRs with previously stable graft function who then suffer acute graft dysfunction, a high urinary NGAL ... there is currently no evidence to support the routine serial measurement of blood or urinary NGAL to detect subclinical acute ... is a protein expressed by kidney tubular cells in response to ischemia, but may also be an early indicator of immunological ... Acute-Phase Proteins, Biomarkers, Delayed Graft Function, Graft Rejection, Humans, Kidney Transplantation, Lipocalin-2, ...
Echocardiography, spirometry, and systemic acute-phase inflammatory proteins in smokers with COPD or CHF: an observational ... In the last 20 years she deals with the long pentraxin PTX3, expressing the protein and characterizing the biochemical features ... Inflammation and thrombosis in essential thrombocythemia and polycythemia vera: different role of C-reactive protein and ... recombinant protein, antibodies) for the identification of MSF as possible marker of TAM and prognostic tool. ...
Akute-Phase-Protein (de); Proteínas de fase aguda (pt); Acute-phase protein (en); بروتين طور حاد (ar); 急性期蛋白 (zh); ... Proteine de phase aigue (fr); Akute-Phase-Proteine, Akut-Phase-Protein (de); Akutfasproteiner (sv); protein akutního stavu, ... Proteínas de fase aguda (es); Beljakovina akutne faze (sl); Protéine de phase aiguë (fr); protein akutní fáze (cs); ... Retrieved from "https://commons.wikimedia.org/w/index.php?title=Category:Acute_phase_proteins&oldid=315167790" ...
Acute-phase response: These proteins, collectively called acute-phase proteins, bind to bacteria and, by doing so, activate ... The acute-phase proteins act similarly to antibodies but are more democratic-that is, they do not distinguish between pathogens ... Other articles where Acute-phase protein is discussed: immune system: ... In immune system: Acute-phase response. These proteins, collectively called acute-phase proteins, bind to bacteria and, by ...
Acute-phase proteins and other systemic responses to inflammation.. Gabay C1, Kushner I. ... Acute-Phase Proteins/analysis. *Acute-Phase Proteins/physiology*. *Acute-Phase Reaction/physiopathology* ...
Amyloidosis and Acute Phase Proteins is part of the Division of Medicine. *Information for Patients ... Normal healthy proteins are cleared away at about the same rate that they are produced, but proteins that have formed amyloid ... The amyloid‑forming protein in AA amyloidosis is called serum amyloid A protein (SAA); the concentration of SAA in the blood ... The amyloid precursor protein is a blood protein called leucocyte chemotactic factor 2 (LECT2). This condition has been ...
This response is called the acute-phase reaction (also called acute-phase response). The terms acute-phase protein and acute- ... Acute-phase proteins (APPs) are a class of proteins whose plasma concentrations increase (positive acute-phase proteins) or ... Increased acute-phase proteins from the liver may also contribute to the promotion of sepsis. Positive acute-phase proteins ... Inflammation and Acute Phase Proteins in Haemostasis. Acute Phase Proteins [Internet]. 2013 [cited 15 October 2015];. Available ...
... Biochem Biophys Res Commun. 1998 Jan 26;242(3):480-3. doi: 10.1006/ ... led us to investigate whether it can be regulated as an acute phase protein (APP). Ang concentration in the serum of mice ... placed into the acute phase by injection with 3% thioglycollate do indeed increase transiently as is typical for APPs. Moreover ... a liver-specific rise and subsequent fall in Ang mRNA transcripts also follows entrance into acute inflammation. We conclude ...
Kimura, T.; Yamamoto, K., 1987: Production of acute phase proteins in the liver. Mode of distribution of acute phase proteins ... Agglutination of intravenous lipid emulsions by acute phase proteins of inflammation. Agglutination of intravenous fat ... Agglutination of intravenous fat emulsions by acute inflammation phase proteins. Agglutination of intravenous fat emulsions ( ... Agglutination of intravenous fat emulsions by acute-phase proteins. Pathologie Biologie 36(7): 877-881. Agglutination of ...
Human serum amyloid A (SAA) protein: a prominent acute-phase reactant for clinical practice.. Malle E1, De Beer FC. ... Acute-phase protein concentrations have been advocated as objective biochemical indices of disease activity in a number of ... It is as sensitive a marker for the acute-phase as C-reactive protein (CRP). Recent studies indicate that SAA is the most ... Observations that the acutephase response is tightly coupled to lipoprotein abnormalities and the fact that acute-SAA proteins ...
Three acute phase proteins (APP); haptoglobin (Hp), mammary associated serum amyloid A3 (M-SAA3) and C-reactive protein (CRP) ... Acute phase proteins, proteomics and metabolomics in the diagnosis of bovine mastitis ...
Increases in the serum acute phase proteins after ozone exposure are associated with induction of genes in the lung but not ... Increases in the serum acute phase proteins after ozone exposure are associated with induction of genes in the lung but not ... EPA Home » Science Inventory » Increases in the serum acute phase proteins after ozone exposure are associated with induction ... Ozone exposure increased positive acute phase proteins in the serum, such as α-1-acid glycoprotein, AGP; α-2-macroglobulin, A2M ...
Plasma Proteome Dynamics: Analysis of Lipoproteins and Acute Phase Response Proteins with 2H2O Metabolic Labeling. Ling Li, ... We applied this technique to measure the synthesis rates of several plasma lipoproteins and acute phase response proteins in ... In this study, we mainly concentrated on several plasma acute phase response proteins and lipoproteins and evaluated the ... The kinetics of different plasma lipoproteins and acute phase response proteins. To emphasize the differences between kinetics ...
Elevated Levels of Acute-Phase Proteins and Plasminogen Activator Inhibitor-1 Predict the Development of Type 2 Diabetes. The ... Elevated Levels of Acute-Phase Proteins and Plasminogen Activator Inhibitor-1 Predict the Development of Type 2 Diabetes ... Elevated Levels of Acute-Phase Proteins and Plasminogen Activator Inhibitor-1 Predict the Development of Type 2 Diabetes ... Elevated Levels of Acute-Phase Proteins and Plasminogen Activator Inhibitor-1 Predict the Development of Type 2 Diabetes ...
Can acute phase proteins predict survival in ventilated patients with acute exacerbation of COPD?. Gamal Agmy, Hoda Maghlouf, ... PURPOSE: The aim of this study was to assess the correlation between acute phase proteins [High sensitivity C-reactive protein ... Can acute phase proteins predict survival in ventilated patients with acute exacerbation of COPD? ... Can acute phase proteins predict survival in ventilated patients with acute exacerbation of COPD? ...
Levels of alpha-2 macroglobulin (A2M) and C-reactive protein (CRP) increased in a significant number of children with acute ... In endemic areas, it is important to detect acute and even asymptomatic infections in children so that specific therapy can be ... The combined analysis of serologic and biochemical parameters can define the different stages of acute infection by Trypanosoma ... a late acute stage, with low IgM levels but high A2M, CRP, anti-Gal, and specific IgG levels. The detection of high IgG levels ...
TNF-α by producing acute phase proteins, including α1-acid glycoprotein (AGP) and α1-antitrypsin (AAT).10 These 2 major acute ... Sepsis score and acute-phase protein response as predictors of outcome in septic surgical patients. Arch Surg. 1987;122:141-146 ... Hepatic acute phase protein mRNA expression 16 hours after either renal I/R, sham operation, administration of 30 μg of LPS IP ... Measurement of Hepatic Acute Phase Protein mRNA Content. Total RNA was extracted from livers and transcribed into cDNA, of ...
A robust and versatile automated glycoanalytical technology for serum antibodies and acute phase proteins: ovarian cancer case ... A robust and versatile automated glycoanalytical technology for serum antibodies and acute phase proteins: ovarian cancer case ... A robust and versatile automated glycoanalytical technology for serum antibodies and acute phase proteins: ovarian cancer case ... A robust and versatile automated glycoanalytical technology for serum antibodies and acute phase proteins: ovarian cancer case ...
Association between circulating IL-6 and acute-phase proteins. Because IL-6 is the principal regulator of hepatic acute-phase ... Acute-phase proteins.. C-reactive protein (CRP) was measured by fluorescent polarization immunoassay using the TDX system ( ... In view of ample in vitro and animal data linking IL-6 with acute-phase protein synthesis in inflammation (28, 29), it was of ... It has been reported that IL-1ra is made by hepatocytes and by cells within the joint and behaves as an acute-phase protein. ...
Preoperative Feeding With a Whey Protein Plus Carbohydrate Drink on the Acute Phase Response and Insulin Resistance. This study ... Effects of preoperative feeding with a whey protein plus carbohydrate drink on the acute phase response and insulin resistance ...
... retinol-binding protein, transferrin, ceruloplasmin, hemopexin, haptoglobin, alpha 1-ac ... Aiming to define the evolution pattern of 10 acute-phase proteins in early infancy, we measured nephelometrically the serum ... 0/Acute-Phase Proteins; 0/Orosomucoid; 0/Retinol-Binding Proteins; 0/Serum Albumin; 0/alpha 1-Antitrypsin; 0/alpha- ... Acute-Phase Proteins / analysis*. Adult. Aging / blood. Ceruloplasmin / analysis. Female. Gestational Age. Humans. Infant. ...
PKA enhances acute-phase insulin proportionately to the glucose challenge.. Acute-phase insulin release is critical for ... 3H), confirming the potency of acute-phase insulin in improving glucose tolerance. Acute-phase insulin release is responsive to ... In contrast, a glucose bolus enhanced acute-phase insulin secretion alone. Acute-phase insulin secretion was increased 3.5-fold ... a critical feature of which is reduced acute-phase insulin release (32,33). The acute phase of insulin release is a major ...
Differential influence of p38 mitogen activated protein kinase (MAPK) inhibition on acute phase protein synthesis in human ... Differential influence of p38 mitogen activated protein kinase (MAPK) inhibition on acute phase protein synthesis in human ... Objective: To investigate the influence of p38 MAPK inhibition on acute phase protein (APP) production, which is dependent on ... Results: Two out of four cell lines produced C reactive protein (CRP), especially after combined IL6 and IL1β stimulation. CRP ...
Acute phase proteins such as C-reactive protein (CRP) and serum amyloid A (SAA) are the biomarkers of inflammation. Hence, the ... interactive effect of drugs of abuse with acute phase proteins in HIV-positive subjects was investigated. Plasma samples were ... Inflammatory proteins such as C-reactive protein (CRP) and serum amyloid A (SAA) are the plasma proteins known as acute phase ... Acute phase proteins such as C-reactive protein (CRP) and serum amyloid A (SAA) are the biomarkers of inflammation. Hence, the ...
  • Neutrophil gelatinase-associated lipocalin (NGAL) is a protein expressed by kidney tubular cells in response to ischemia, but may also be an early indicator of immunological rejection, calcineurin inhibitor toxicity, obstructive nephropathy, subclinical tubulitis or infection. (ox.ac.uk)
  • Glomerular filtration rate and albuminuria for detection and staging of acute and chronic kidney disease in adults: a systematic review. (gmj.ir)
  • Although there is currently no evidence to support the routine serial measurement of blood or urinary NGAL to detect subclinical acute tubular injury, NGAL has the potential to provide useful information to those that care for kidney transplant recipients (KTRs). (ox.ac.uk)
  • TNF-α is also an inducer of the acute phase response (APR), a complex series of reactions executed by the host in the immediate aftermath of injury, trauma, or infection. (ahajournals.org)
  • The aim of the study was to analyze acute phase protein and cathelicidin gene responses to small ruminant lentivirus (SRLV) infection in goats. (biomedcentral.com)
  • An extended response was found for AGP which occurred at a point when the infection was likely to have been transforming from an acute to a chronic phase. (beds.ac.uk)
  • Although called the acute phase proteins their presence in the circulation can persist beyond the immediate post-infection time period, although, as pathological lesions become chronic, the pattern of response can vary. (beds.ac.uk)
  • The, up-regulated gene transcripts or proteins have been further characterized for their potential role in both the cellular and humoral immunity (defense responses) of shrimps in response to WSSV infection. (plos.org)
  • To evaluate serial serum C-reactive protein (CRP) levels for diagnosis of neonatal infection. (aappublications.org)
  • Ang concentration in the serum of mice placed into the acute phase by injection with 3% thioglycollate do indeed increase transiently as is typical for APPs. (nih.gov)
  • Combinations of APPs allowed the detection of disease more sensitively than any individual APP and the best three-protein combinations were CRP, apoA1, pigMAP and CRP, apoA1, Hp, respectively, closely followed by the two-protein combinations CRP, pigMAP and apoA1, pigMAP, respectively. (biomedcentral.com)
  • The aim of this study was the development of a quantitative assay that could support future studies of a panel of acute phase proteins (APPs) in the horse. (jpt.com)
  • Growth performance, behavioral stress markers, and physiological stress markers (neutrophil:lymphocyte ratio and acute phase proteins [APPs]) were measured from birth to Day 58. (aasv.org)
  • The term amyloidosis describes a group of disorders caused by abnormal folding, aggregation and accumulation of certain proteins in the tissues, in an abnormal form known as amyloid deposits. (ucl.ac.uk)
  • These deposits are composed of abnormal protein fibres, the so‑called amyloid fibrils that accumulate more quickly than they are cleared away, and which progressively interfere with the structure and function of affected organs throughout the body. (ucl.ac.uk)
  • Normal healthy proteins are cleared away at about the same rate that they are produced, but proteins that have formed amyloid are broken down only very slowly. (ucl.ac.uk)
  • About 30 different proteins have been found to form amyloid in man, but only a few are associated with clinically significant disease. (ucl.ac.uk)
  • Amyloidosis is classified according to the protein that forms the amyloid fibrils and the clinical picture and symptoms can differ greatly between one amyloid type and another. (ucl.ac.uk)
  • In this condition, amyloid deposits in the bones and joints are formed from a protein called β2‑microglobulin. (ucl.ac.uk)
  • Localised amyloid deposits composed of a protein called Aβ occur in the brains of patients with Alzheimer's disease, but it is not known whether they are the cause of the disease. (ucl.ac.uk)
  • Human serum amyloid A (SAA) protein: a prominent acute-phase reactant for clinical practice. (nih.gov)
  • In the patients with MCTs, C-reactive protein (CRP) and α-1 acid glycoprotein levels increased, with a concurrent drop in serum amyloid A levels. (bmj.com)
  • Modest and similar improvements of C reactive protein, serum amyloid protein A, and erythrocyte sedimentation rate were seen in both study groups. (bmj.com)
  • Acute-phase proteins include C-reactive protein, several complement and coagulation factors, transport proteins, amyloid, and antiprotease enzymes. (tabers.com)
  • ABBR: APP An integral membrane protein concentrated at neuron synapses that is cleaved biochemically into components, one of which is the Alzheimer disease-associated beta amyloid. (tabers.com)
  • The functions of the APP included protease inhibitors, enzymes, transport proteins, coagulation proteins, and modulators of the immune response. (deepdyve.com)
  • Pools of overlapping peptides from antigenic proteins for T-cell stimulation, T-cell expansion in T-cell assays such as ELISPOT, ICS or Flow Cytometry, e.g. for immune monitoring. (jpt.com)
  • Acute phase proteins ( APP ) are the cornerstone to the innate immune response. (miami.edu)
  • Adapter proteins participate in the immune response by acting as a bridge for enzymes in the signaling pathway needed to activate lymphocytes and initiate a response to an antigen. (tabers.com)
  • During humoral immune responses, proteins called antibodies, which can bind to and destroy pathogens, are secreted into the blood and other body fluids. (encyclopedia.com)
  • In contrast, C-reactive protein (with a half-life of 6-8 hours) rises rapidly and can quickly return to within the normal range if treatment is employed. (wikipedia.org)
  • For example, in active systemic lupus erythematosus, one may find a raised ESR but normal C-reactive protein. (wikipedia.org)
  • It is as sensitive a marker for the acute-phase as C-reactive protein (CRP). (nih.gov)
  • C-reactive protein was expressed highly in the liver but minimal expression was noted in the lung (not affected by ozone in lung or liver). (epa.gov)
  • In the present study, we investigated the relation of C-reactive protein (CRP), fibrinogen, and plasminogen activator inhibitor 1 (PAI-1) to incident type 2 diabetes during a 5-year period in the Insulin Resistance Atherosclerosis Study (IRAS). (diabetesjournals.org)
  • The aim of this study was to assess the correlation between acute phase proteins [High sensitivity C-reactive protein (CRP) and prealbumin (PA)] and mortality in ventilated patients with COPD. (ersjournals.com)
  • Two out of four cell lines produced C reactive protein (CRP), especially after combined IL6 and IL1β stimulation. (bmj.com)
  • 0.05) in weight, body mass index (BMI) waist hip ratio, fasting blood sugar, C-reactive protein, total cholesterol, triglyceride, triglyceride/high density lipoprotein (TG/HDLC), low density lipoprotein/ high density lipoprotein (LDLC/HDLC) and TC/HDLC in type 2 diabetic mellitus patients when compared with the controls. (ajol.info)
  • All infected pigs developed sepsis characterized by fever, neutrophilia, increased serum levels of C-reactive protein (CRP) and interleukin (IL)-6, and decreased levels of serum iron. (dtu.dk)
  • We examined the reliability of C-reactive protein (CRP), procalcitonin (PCT), and WBC for diagnosis of infections during SE. (biomedcentral.com)
  • C-reactive protein (CRP) is an acute-phase serum protein that has been shown to help protect the host from several bacterial pathogens, which it recognizes by binding to phosphorylcholine (PC) on their surfaces. (surrey.ac.uk)
  • For example, C-reactive protein (CRP) can increase as much as 1000-fold in humans and dogs but it increases by only 50% in rats. (lifediagnostics.com)
  • BACKGROUND: Elevated levels of C-reactive protein (CRP) are associated with an unfavorable clinical outcome in patients with unstable angina. (ox.ac.uk)
  • Serum interleukin-6, procalcitonin and C-reactive protein levels in subjects with active Behçet's disease. (semanticscholar.org)
  • High-Sensitivity C-Reactive Protein Is Associated With Incident Type 2 Diabetes Among African Americans: The Jackson Heart Study Diabetes Care. (edu.krd)
  • Increased concentration of C-reactive protein in obese patients with type 2 diabetes is associated with obesity and presence of diabetes but not with macrovascular and microvascular complications or glycemic control. (edu.krd)
  • In clinical medicine the erythrocyte sedimentation rate or serum C-reactive protein level sometimes is used as a marker of increased amounts of acute-phase proteins. (tabers.com)
  • 216.5 μg/mL had higher rectal (P = 0.017) and vaginal temperature (P = 0.02) than sows with C-reactive protein ≤ 216.5 μg/mL. (fu-berlin.de)
  • C-reactive protein (CRP) is one of the most frequently measured acute-phase proteins in clinical medicine. (beds.ac.uk)
  • Fever and results of serial measurements of C-reactive protein, erythrocyte sedimentation rate, white blood cell counts, and platelet counts were related to the clinical course of infective endocarditis.Fever persisted or recurred in 108 episodes (57%) despite appropriate antibiotic treatment. (gu.se)
  • Elevations in C-reactive protein levels were significantly prolonged in the episodes with complicated courses compared with the episodes with uncomplicated courses, while mean erythrocyte sedimentation rate remained unchanged during treatment, not differentiating between complicated and uncomplicated episodes.Fever during treatment must be analyzed in terms of persistence and recurrence to provide a basis for clinical decisions. (gu.se)
  • Serial measurements of C-reactive protein are useful to monitor the response to antimicrobial therapy and to detect complications, while serial determinations of erythrocyte sedimentation rate are of no value. (gu.se)
  • In the mid 1990s, immunoassays for C-reactive protein (CRP), with greater sensitivity than those previously in routine use, revealed that increased CRP values, even within the range previously considered normal, strongly predict future coronary events. (jci.org)
  • Compared with men in the bottom third of baseline measurements of C reactive protein, men in the top third had an odds ratio for coronary heart disease of 2.13 (95% confidence interval 1.38 to 3.28) after age, town, smoking, vascular risk factors, and indicators of socioeconomic status were adjusted for. (bmj.com)
  • 2 6 A variety of mechanisms by which C reactive protein might directly promote vascular disease have been proposed, 7 but none is proved. (bmj.com)
  • The figures and table below illustrate the serum levels of acute phase proteins in lipopolysaccharide (LPS) treated rats using ELISA kits manufactured by Life Diagnostics, Inc. Sprague Dawley (~200g) rats were injected (i.p.) with 1 mg/kg LPS in saline. (lifediagnostics.com)
  • Depressed women in perimenopause, when being compared to non-depressed women, did not differ as to serum levels of acute-phase proteins. (beds.ac.uk)
  • This study investigates the contribution of the acute phase proteins α 1 -acid glycoprotein (AGP) and α 1 -antitrypsin (AAT) to the protective effect of ischemic preconditioning in the kidney. (ahajournals.org)
  • 3 9 During the APR, liver cells and various epithelial cells respond to increasing levels of, among others, TNF-α by producing acute phase proteins, including α 1 -acid glycoprotein (AGP) and α 1 -antitrypsin (AAT). (ahajournals.org)
  • FIP is characterized by the overexpression of an acute phase protein, the α1-acid glycoprotein (AGP). (usda.gov)
  • Our study demonstrates an approach capable of investigating how protein glycosylation fine-tunes protein-drug interactions at the glycan-specific level and will prove universally useful for studying glycoprotein interactions. (pnas.org)
  • Elucidating glycoprotein microheterogeneity and relating subtle changes in the glycan structural repertoire to changes in protein-protein, or protein-small molecule interactions, remains a significant challenge in glycobiology. (pnas.org)
  • Taken together, our results not only elucidate how glycoprotein microheterogeneity regulates protein-drug/protein interactions but also inform the pharmacokinetics of plasma proteins, many of which are drug targets, and whose glycosylation status changes in various disease states. (pnas.org)
  • p38 MAPK inhibitors, especially, have been developed and are now in phase II clinical trials for rheumatoid arthritis (RA). (bmj.com)
  • Analysis of the EKG recordings and blood parameters are under way, including detailed analyses of HRV and repolarization, and acute-phase proteins using methodology identical to the human clinical studies. (thefreedictionary.com)
  • Multivariate linear and logistic regression was used to study the association between acute-phase protein levels in relation to clinical, neuropsychiatric, cognitive, and psychotropic use characteristics in a cohort consisting of 122 HD mutation carriers and 42 controls at first biomarker measurement, and 85 HD mutation carriers and 32 controls at second biomarker measurement. (cdc.gov)
  • It was the year of 1969 when this monograph was originally published in Japanese by Professor TADASHI KAWAI, titled as "The Plasma Proteins, Their Fundamental and Clinical Aspects. (springer.com)
  • This article provides information about CRP as a protein and an acute-phase reactant, and a knowledge-based framework for interpretation and analysis of clinical observations of CRP in relation to cardiovascular and other diseases. (jci.org)
  • LBP is a nonspecific marker of the acute phase response and cannot be used as a diagnostic tool for differentiating between infectious and noninfectious etiologies of SIRS. (biomedcentral.com)
  • Therefore, it concluded that C. pseudotuberculosis and PLD can influence the level of acute phase proteins in goats. (biomedcentral.com)
  • Can acute phase proteins predict survival in ventilated patients with acute exacerbation of COPD? (ersjournals.com)