Proteins that are secreted into the blood in increased or decreased quantities by hepatocytes in response to trauma, inflammation, or disease. These proteins can serve as inhibitors or mediators of the inflammatory processes. Certain acute-phase proteins have been used to diagnose and follow the course of diseases or as tumor markers.
Plasma glycoproteins that form a stable complex with hemoglobin to aid the recycling of heme iron. They are encoded in man by a gene on the short arm of chromosome 16.
An early local inflammatory reaction to insult or injury that consists of fever, an increase in inflammatory humoral factors, and an increased synthesis by hepatocytes of a number of proteins or glycoproteins usually found in the plasma.
An ACUTE PHASE REACTION protein present in low concentrations in normal sera, but found at higher concentrations in sera of older persons and in patients with AMYLOIDOSIS. It is the circulating precusor of amyloid A protein, which is found deposited in AA type AMYLOID FIBRILS.
Glycoprotein found in alpha(1)-globulin region in human serum. It inhibits chymotrypsin-like proteinases in vivo and has cytotoxic killer-cell activity in vitro. The protein also has a role as an acute-phase protein and is active in the control of immunologic and inflammatory processes, and as a tumor marker. It is a member of the serpin superfamily.
The concrete oleoresin obtained from Pinus palustris Mill. (Pinaceae) and other species of Pinus. It contains a volatile oil, to which its properties are due, and to which form it is generally used. (Dorland, 28th ed) Turpentine is used as a solvent and an experimental irritant in biomedical research. Turpentine toxicity is of medical interest.
Plasma glycoprotein member of the serpin superfamily which inhibits TRYPSIN; NEUTROPHIL ELASTASE; and other PROTEOLYTIC ENZYMES.
A plasma protein that circulates in increased amounts during inflammation and after tissue damage.
Amyloid P component is a small, non-fibrillar glycoprotein found in normal serum and in all amyloid deposits. It has a pentagonal (pentaxin) structure. It is an acute phase protein, modulates immunologic responses, inhibits ELASTASE, and has been suggested as an indicator of LIVER DISEASE.
A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.
Plasma glycoprotein clotted by thrombin, composed of a dimer of three non-identical pairs of polypeptide chains (alpha, beta, gamma) held together by disulfide bonds. Fibrinogen clotting is a sol-gel change involving complex molecular arrangements: whereas fibrinogen is cleaved by thrombin to form polypeptides A and B, the proteolytic action of other enzymes yields different fibrinogen degradation products.
A species of gram-negative bacteria isolated from the SYNOVIAL FLUID; LYMPH NODES; and MUCOUS MEMBRANE secretions in diseased SWINE. It causes nonsuppurative ARTHRITIS.
Glycoproteins with a molecular weight of approximately 620,000 to 680,000. Precipitation by electrophoresis is in the alpha region. They include alpha 1-macroglobulins and alpha 2-macroglobulins. These proteins exhibit trypsin-, chymotrypsin-, thrombin-, and plasmin-binding activity and function as hormonal transporters.
Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.
Nematodes parasitic in the bronchi of herbivorous animals.
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.
Serum proteins that have the most rapid migration during ELECTROPHORESIS. This subgroup of globulins is divided into faster and slower alpha(1)- and alpha(2)-globulins.
Dryness of the eye surfaces caused by deficiency of tears or conjunctival secretions. It may be associated with vitamin A deficiency, trauma, or any condition in which the eyelids do not close completely.
Ligand-binding assays that measure protein-protein, protein-small molecule, or protein-nucleic acid interactions using a very large set of capturing molecules, i.e., those attached separately on a solid support, to measure the presence or interaction of target molecules in the sample.
A tetrameric protein, molecular weight between 50,000 and 70,000, consisting of 4 equal chains, and migrating on electrophoresis in 3 fractions more mobile than serum albumin. Its concentration ranges from 7 to 33 per cent in the serum, but levels decrease in liver disease.
Infections with bacteria of the genus ACTINOBACILLUS.
Water-soluble proteins found in egg whites, blood, lymph, and other tissues and fluids. They coagulate upon heating.
A diverse family of extracellular proteins that bind to small hydrophobic molecules. They were originally characterized as transport proteins, however they may have additional roles such as taking part in the formation of macromolecular complexes with other proteins and binding to CELL SURFACE RECEPTORS.
A glycoprotein that is central in both the classical and the alternative pathway of COMPLEMENT ACTIVATION. C3 can be cleaved into COMPLEMENT C3A and COMPLEMENT C3B, spontaneously at low level or by C3 CONVERTASE at high level. The smaller fragment C3a is an ANAPHYLATOXIN and mediator of local inflammatory process. The larger fragment C3b binds with C3 convertase to form C5 convertase.
Elements of limited time intervals, contributing to particular results or situations.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
An iron-binding beta1-globulin that is synthesized in the LIVER and secreted into the blood. It plays a central role in the transport of IRON throughout the circulation. A variety of transferrin isoforms exist in humans, including some that are considered markers for specific disease states.
A glycoprotein that is important in the activation of CLASSICAL COMPLEMENT PATHWAY. C4 is cleaved by the activated COMPLEMENT C1S into COMPLEMENT C4A and COMPLEMENT C4B.
A species of gram-negative, facultatively anaerobic coccobacillus-shaped bacteria that has been isolated from pneumonic lesions and blood. It produces pneumonia with accompanying fibrinous pleuritis in swine.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)
Diseases of domestic swine and of the wild boar of the genus Sus.
Failure or imperfection of vision at night or in dim light, with good vision only on bright days. (Dorland, 27th ed)
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
A nutritional condition produced by a deficiency of VITAMIN A in the diet, characterized by NIGHT BLINDNESS and other ocular manifestations such as dryness of the conjunctiva and later of the cornea (XEROPHTHALMIA). Vitamin A deficiency is a very common problem worldwide, particularly in developing countries as a consequence of famine or shortages of vitamin A-rich foods. In the United States it is found among the urban poor, the elderly, alcoholics, and patients with malabsorption. (From Cecil Textbook of Medicine, 19th ed, p1179)
Measurement of rate of settling of erythrocytes in anticoagulated blood.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A signal transducer and activator of transcription that mediates cellular responses to INTERLEUKIN-6 family members. STAT3 is constitutively activated in a variety of TUMORS and is a major downstream transducer for the CYTOKINE RECEPTOR GP130.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Disease having a short and relatively severe course.
A cytokine receptor that acts through the formation of oligomeric complexes of itself with a variety of CYTOKINE RECEPTORS.
Cell surface receptors that are specific for INTERLEUKIN-6. They are present on T-LYMPHOCYTES, mitogen-activated B-LYMPHOCYTES, and peripheral MONOCYTES. The receptors are heterodimers of the INTERLEUKIN-6 RECEPTOR ALPHA SUBUNIT and the CYTOKINE RECEPTOR GP130.
An anti-inflammatory 9-fluoro-glucocorticoid.
A primary malignant neoplasm of epithelial liver cells. It ranges from a well-differentiated tumor with EPITHELIAL CELLS indistinguishable from normal HEPATOCYTES to a poorly differentiated neoplasm. The cells may be uniform or markedly pleomorphic, or form GIANT CELLS. Several classification schemes have been suggested.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Soluble factors which stimulate growth-related activities of leukocytes as well as other cell types. They enhance cell proliferation and differentiation, DNA synthesis, secretion of other biologically active molecules and responses to immune and inflammatory stimuli.
Toxins closely associated with the living cytoplasm or cell wall of certain microorganisms, which do not readily diffuse into the culture medium, but are released upon lysis of the cells.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Proteins prepared by recombinant DNA technology.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus.
The endogenous compounds that mediate inflammation (AUTACOIDS) and related exogenous compounds including the synthetic prostaglandins (PROSTAGLANDINS, SYNTHETIC).
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A class of statistical methods applicable to a large set of probability distributions used to test for correlation, location, independence, etc. In most nonparametric statistical tests, the original scores or observations are replaced by another variable containing less information. An important class of nonparametric tests employs the ordinal properties of the data. Another class of tests uses information about whether an observation is above or below some fixed value such as the median, and a third class is based on the frequency of the occurrence of runs in the data. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1284; Corsini, Concise Encyclopedia of Psychology, 1987, p764-5)
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules.
A chronic systemic disease, primarily of the joints, marked by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Etiology is unknown, but autoimmune mechanisms have been implicated.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.
Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Systemic inflammatory response syndrome with a proven or suspected infectious etiology. When sepsis is associated with organ dysfunction distant from the site of infection, it is called severe sepsis. When sepsis is accompanied by HYPOTENSION despite adequate fluid infusion, it is called SEPTIC SHOCK.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Tumors or cancer of the LIVER.
Glycoproteins found on the membrane or surface of cells.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Transport proteins that carry specific substances in the blood or across cell membranes.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
The rate dynamics in chemical or physical systems.
The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Established cell cultures that have the potential to propagate indefinitely.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
The systematic study of the complete complement of proteins (PROTEOME) of organisms.
Infection with the protozoan parasite TRYPANOSOMA CRUZI, a form of TRYPANOSOMIASIS endemic in Central and South America. It is named after the Brazilian physician Carlos Chagas, who discovered the parasite. Infection by the parasite (positive serologic result only) is distinguished from the clinical manifestations that develop years later, such as destruction of PARASYMPATHETIC GANGLIA; CHAGAS CARDIOMYOPATHY; and dysfunction of the ESOPHAGUS or COLON.
An acute, febrile, mucocutaneous condition accompanied by swelling of cervical lymph nodes in infants and young children. The principal symptoms are fever, congestion of the ocular conjunctivae, reddening of the lips and oral cavity, protuberance of tongue papillae, and edema or erythema of the extremities.
The protein complement of an organism coded for by its genome.
Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group.
The agent of South American trypanosomiasis or CHAGAS DISEASE. Its vertebrate hosts are man and various domestic and wild animals. Insects of several species are vectors.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.

Predominant immunoglobulin A response to phase II antigen of Coxiella burnetii in acute Q fever. (1/1561)

Diagnosis of acute Q fever is usually confirmed by serology, on the basis of anti-phase II antigen immunoglobulin M (IgM) titers of >/=1:50 and IgG titers of >/=1:200. Phase I antibodies, especially IgG and IgA, are predominant in chronic forms of the disease. However, between January 1982 and June 1998, we observed anti-phase II antigen IgA titers of >/=1:200 as the sole or main antibody response in 10 of 1,034 (0.96%) patients with acute Q fever for whom information was available. In order to determine whether specific epidemiological or clinical factors were associated with these serological profiles, we conducted a retrospective case-control study that included completion of a standardized questionnaire, which was given to 40 matched controls who also suffered from acute Q fever. The mean age of patients with elevated phase II IgA titers was significantly higher than that usually observed for patients with acute Q fever (P = 0.026); the patients were also more likely than controls to live in rural areas (P = 0.026) and to have increased levels of transaminase in blood (P = 0.03). Elevated IgA titers are usually associated with chronic Q fever and are directed mainly at phase I antigens. Although the significance of our findings is unexplained, we herein emphasize the fact that IgA antibodies are not specific for chronic forms of Q fever and that they may occasionally be observed in patients with acute disease. Moreover, as such antibody profiles may not be determined by most laboratories, which test only for total antibody titers to phase I and II antigens, the three isotype-specific Ig titers should be determined as the first step in diagnosing Q fever.  (+info)

The STAT3-independent signaling pathway by glycoprotein 130 in hepatic cells. (2/1561)

Interleukin (IL)-6 is a major regulator of hepatic acute-phase plasma protein (APP) genes. The membrane-proximal 133-amino acid cytoplasmic domain of glycoprotein (gp) 130, containing one copy of the Box3 motif, is sufficient to transmit a productive signal to endogenous APP genes in rat hepatoma H-35 cells. In contrast, a mutant gp130 domain lacking the Box3 motif activates Janus kinases to a normal level but fails to activate signal transducer and activator of transcription 3 and to up-regulate a number of APP genes, including thiostatin, fibrinogen, hemopexin, and haptoglobin. However, in the absence of Box3, gp130 still stimulates the expression of alpha2-macroglobulin and synergizes with IL-1 to up-regulate alpha1-acid glycoprotein. The Box3 motif is not required for activation of the SH2-containing protein tyrosine phosphatase 2 or the mitogen-activated protein kinase (MAPK), nor is the immediate induction of egr-1 and junB significantly altered. Surprisingly, gp130 without any functional Box3 stimulates prolonged activation of MAPK, leading to an extended period of up-regulation of egr-1 and to an extracellularly regulated kinase-mediated reduction in the IL-6-stimulated production of thiostatin. IL-6 reduces proliferation of H-35 cells through signaling by the Box3. In addition, cells expressing Box3-deficient gp130 showed distinct morphologic changes upon receptor activation. Taken together, these results indicate that Box3-derived and Box3-independent signals cooperate in the control of hepatic APP genes and that Box3 may be involved in the modulation of MAPK activity in gp130 signaling.  (+info)

Lipopolysaccharide stimulates HepG2 human hepatoma cells in the presence of lipopolysaccharide-binding protein via CD14. (3/1561)

Lipopolysaccharide (LPS)-binding protein (LBP), an opsonin for activation of macrophages by bacterial LPS, is synthesized in hepatocytes and is known to be an acute phase protein. Recently, cytokine-induced production of LBP was reported to increase 10-fold in hepatocytes isolated from LPS-treated rats, compared with those from normal rats. However, the mechanism by which the LPS treatment enhances the effect of cytokines remains to be clarified. In the present study, we examined whether LPS alone or an LPS/LBP complex directly stimulates the hepatocytes, leading to acceleration of the cytokine-induced LBP production. HepG2 cells (a human hepatoma cell line) were shown to express CD14, a glycosylphosphatidylinositol-anchored LPS receptor, by both RT/PCR and flow cytometric analyses. An LPS/LBP complex was an effective stimulator for LBP and CD14 production in HepG2 cells, but stimulation of the cells with either LPS or LBP alone did not significantly accelerate the production of these proteins. The findings were confirmed by semiquantitative RT/PCR analysis of mRNA levels of LBP and CD14 in HepG2 cells after stimulation with LPS alone and an LPS/LBP complex. In addition, two monoclonal antibodies (mAbs) to CD14 (3C10 and MEM-18) inhibited LPS/LBP-induced cellular responses of HepG2 cells. Furthermore, prestimulation of HepG2 cells with LPS/LBP augmented cytokine-induced production and gene expression of LBP and CD14. All these findings suggest that an LPS/LBP complex, but not free LPS, stimulates HepG2 cells via CD14 leading to increased basal and cytokine-induced LBP and CD14 production.  (+info)

Heme and acute inflammation role in vivo of heme in the hepatic expression of positive acute-phase reactants in rats. (4/1561)

Acute-phase protein synthesis in the liver during inflammation is regulated via cytokines and glucocorticoids. Using quantitative reverse transcription (RT)-PCR analysis and immunoassay, we explored, in the rat, the response of the acute-phase protein, alpha-2 macroglobulin (A2M), after systemic inflammation induced by lipopolysaccharide (LPS) or localized inflammation induced by turpentine oil (TO). The results indicate that synthesis of A2M is higher following TO-induced inflammation than LPS-induced inflammation and is not correlated with interleukin (IL)-6 or glucocorticoid levels. We studied the putative role of heme in this differential A2M expression following localized vs. systemic inflammation; addition of heme during LPS-induced inflammation can boost the expression of A2M, whereas blocking heme synthesis (by succinyl acetone) or enhancing its consumption in parallel biosynthetic pathways (cytochrome P450 induction by phenobarbital) decreases A2M expression. This decrease was abolished by exogenous heme supplementation. Finally, we demonstrate that heme supplementation is also able to increase the A2M response in female rats to a level similar to that in male rats providing a new insight into the puzzling sexual dimorphism observed previously during localized inflammation. We propose that heme should be considered a new regulatory element in controlling liver A2M expression during inflammation.  (+info)

Endotoxin interactions with lipopolysaccharide-responsive cells. (5/1561)

Recent work has identified two proteins that work together to enable many cell types to respond to endotoxin. These two proteins, lipopolysaccharide (LPS) binding protein (LBP) and CD14, also participate in cellular internalization of endotoxin, which may occur independently of cellular activation. Current work with antibodies to LBP and CD14 as well as "knockout" mice in the context of LPS-initiated endotoxic shock suggests that inhibition of this pathway could be therapeutically useful. These observations point to the need to identify new molecules that mediate LPS-initiated transmembrane signaling and internalization of LPS-protein complexes.  (+info)

Lipopolysaccharide-coated erythrocytes activate human neutrophils via CD14 while subsequent binding is through CD11b/CD18. (6/1561)

Interaction of LPS with monocytes and neutrophils is known to occur via CD14 and is strongly enhanced by LPS-binding protein (LBP). Integrins as well as CD14 play a role in the interaction of erythrocytes (E) coated with LPS or whole Gram-negative bacteria with phagocytes. We reasoned that the density of LPS on a particle is an important determinant in these interactions. Therefore, E were coated with different concentrations of LPS (ELPS). The binding of these ELPS to neutrophils was evaluated by flow cytometry. Simultaneously, we measured fMLP receptor expression to evaluate neutrophil activation. ELPS only bound to neutrophils in the presence of LBP. Blocking CD14 inhibited both activation and binding, whereas blocking complement (C) receptor 3 (CR3) inhibited binding but not activation. TNF activation restored ELPS binding in CD14-blocked cells but not in cells in which CR3 was blocked. Salmonella minnesota did bind to neutrophils independent of CR3 or CD14. The addition of LBP enhanced binding twofold, and this surplus was dependent upon CD14 but not on CR3. We conclude that ELPS interact with neutrophils via CD14, initially giving rise to cell activation; subsequently, binding is solely mediated by activated CR3.  (+info)

Membrane-anchored forms of lipopolysaccharide (LPS)-binding protein do not mediate cellular responses to LPS independently of CD14. (7/1561)

Inflammatory responses of myeloid cells to LPS are mediated through CD14, a glycosylphosphatidylinositol-anchored receptor that binds LPS. Since CD14 does not traverse the plasma membrane and alternatively anchored forms of CD14 still enable LPS-induced cellular activation, the precise role of CD14 in mediating these responses remains unknown. To address this, we created a transmembrane and a glycosylphosphatidylinositol-anchored form of LPS-binding protein (LBP), a component of serum that binds and transfers LPS to other molecules. Stably transfected Chinese hamster ovary (CHO) fibroblast and U373 astrocytoma cell lines expressing membrane-anchored LBP (mLBP), as well as separate CHO and U373 cell lines expressing membrane CD14 (mCD14), were subsequently generated. Under serum-free conditions, CHO and U373 cells expressing mCD14 responded to as little as 0.1 ng/ml of LPS, as measured by NF-kappaB activation as well as ICAM and IL-6 production. Conversely, the vector control and mLBP-expressing cell lines did not respond under serum-free conditions even in the presence of more than 100 ng/ml of LPS. All the cell lines exhibited responses to less than 1 ng/ml of LPS in the presence of the soluble form of CD14, demonstrating that they are still capable of LPS-induced activation. Taken together, these results demonstrate that mLBP, a protein that brings LPS to the cell surface, does not mediate cellular responses to LPS independently of CD14. These findings suggest that CD14 performs a more specific role in mediating responses to LPS than that of simply bringing LPS to the cell surface.  (+info)

Blood concentrations of pancreatitis associated protein in neonates: relevance to neonatal screening for cystic fibrosis. (8/1561)

AIM: To determine whether pancreatitis associated protein (PAP) is a marker for cystic fibrosis which could be used in neonatal screening for the disease. METHODS: PAP was assayed on screening cards from 202,807 neonates. Babies with PAP > or = 15 ng/ml, or > or = 11.5 ng/ml and immunoreactive trypsinogen (IRT) > or = 700 ng/ml were recalled for clinical examination, sweat testing, and cystic fibrosis transmembrane regulator (CFTR) gene analysis. RESULTS: Median PAP value was 2.8 ng/ml. Forty four cases of cystic fibrosis were recorded. Recalled neonates (n = 398) included only 11 carriers. A receiver operating characteristic curve analysis showed that PAP above 8.0 ng/ml would select 0.76% of babies, including all those with cystic fibrosis, except for one with meconium ileus and two with mild CFTR mutations. Screening 27,146 babies with both PAP and IRT showed that only 0.12% had PAP > 8.0 ng/ml and IRT > 700 ng/ml, including all cases of cystic fibrosis. CONCLUSION: PAP is increased in most neonates with cystic fibrosis and could be used for CF screening. Its combination with IRT looks promising.  (+info)

Acute-phase proteins (APPs) are a class of proteins whose plasma concentrations increase (positive acute-phase proteins) or decrease (negative acute-phase proteins) in response to inflammation. This response is called the acute-phase reaction (also called acute-phase response). The terms acute-phase protein and acute-phase reactant (APR) are often used synonymously, although some APRs are (strictly speaking) polypeptides rather than proteins. In response to injury, local inflammatory cells (neutrophil granulocytes and macrophages) secrete a number of cytokines into the bloodstream, most notable of which are the interleukins IL1, IL6 and IL8, and TNFα. The liver responds by producing a large number of acute-phase reactants. At the same time, the production of a number of other proteins is reduced; these proteins are, therefore, referred to as negative acute-phase reactants. Increased acute-phase proteins from the liver may also contribute to the promotion of sepsis. Positive acute-phase ...
Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene.[5][6] LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4.[7] The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma ...
The acute phase protein (APP) response was evaluated after prolonged transportation of pigs under commercial conditions. Elevated serum APP concentrations were observed in two groups of boars immediately after their arrival at a destination farm compared with within-animal control samples obtained one month later. The effect was more pronounced in the first group of pigs conveyed under average transport conditions (Transport 1, 24 h), although the second group was transported for a longer time period (Transport 2, 48 h) but in superior transport conditions. In a second trial, pigs were sampled before transport, on arrival at an abattoir (following 12 h transport), and at the slaughter-line (after 6 h lairage). Significant increases in major acute phase protein (Pig-MAP), haptoglobin, serum amyloid A, C-reactive protein, and a decrease in apolipoprotein A-I, were observed at slaughter. The results demonstrate that shipment of pigs by road can result in an APP response that is probably related to ...
The aim of our study was to investigate the predictive value of the biomarkers interleukin 6 (IL-6), interleukin 10 (IL-10) and lipopolysaccharide-binding protein (LBP) compared with clinical CRB and CRB-65 severity scores in patients with community-acquired pneumonia (CAP). Samples and data were obtained from patients enrolled into the German CAPNETZ study group. Samples (blood, sputum and urine) were collected within 24 h of first presentation and inclusion in the CAPNETZ study, and CRB and CRB-65 scores were determined for all patients at the time of enrollment. The combined end point representative of a severe course of CAP was defined as mechanical ventilation, intensive care unit treatment and/or death within 30 days. Overall, a total of 1,000 patients were enrolled in the study. A severe course of CAP was observed in 105 (10.5%) patients. The highest IL-6, IL-10 and LBP concentrations were found in patients with CRB-65 scores of 3-4 or CRB scores of 2-3. IL-6 and LBP levels on enrollment in the
Background: Inhibition of intracellular signal transduction is considered to be an interesting target for treatment in inflammation. p38 MAPK inhibitors, especially, have been developed and are now in phase II clinical trials for rheumatoid arthritis (RA).. Objective: To investigate the influence of p38 MAPK inhibition on acute phase protein (APP) production, which is dependent on both JAK/STAT and p38 MAPK pathways.. Methods: The effects of p38 MAPK inhibition on APP production and mRNA expression in four human hepatoma cell lines was investigated, after stimulation with interleukin (IL)6 and/or IL1β or tumour necrosis factor α.. Results: Two out of four cell lines produced C reactive protein (CRP), especially after combined IL6 and IL1β stimulation. CRP production was significantly inhibited by the p38 MAPK specific inhibitor RWJ 67657 at 1 μmol/l, which is pharmacologically relevant. Fibrinogen production was also inhibited at 1 μmol/l in all cell lines. Serum amyloid A (SAA) was ...
LBP [LPS (lipopolysaccharide)-binding protein] was discovered approximately 25 years ago. Since then, substantial progress has been made towards our understanding of its function in health and disease. Furthermore, the discovery of a large protein family sharing functional and structural attributes has helped in our knowledge. Still, key questions are unresolved, and here an overview on the old and new findings on LBP is given. LBP is an acute-phase protein of the liver, but is also synthesized in other cells of the organism. While LBP is named after the ability to bind to LPS of Gram-negative bacteria, it also can recognize other bacterial compounds, such as lipopeptides. It has been shown that LBP is needed to combat infections; however, the main mechanism of action is still not clear. New findings on natural genetic variations of LBP leading to functional consequences may help in further elucidating the mechanism of LBP and its role in innate immunity and disease. ...
Acute phase protein levels increase in blood and other bodily fluids as a result of inflammation, tissue injury and disease. They are used as biomarkers of disease in humans, companion animals, livestock and species used in preclinical research. When selecting an acute phase biomarker it is important to appreciate that a good biomarker in one species is not necessarily useful in other species. For example, C-reactive protein (CRP) can increase as much as 1000-fold in humans and dogs but it increases by only 50% in rats. Please contact us if you would like advice regarding biomarker selection. We currently manufacture ELISA kits for measurement of acute phase proteins including CRP, haptoglobin, hemopexin, alpha-1 acid glycoprotein, clusterin, alpha-2-macroglobulin, serum amyloid A (SAA) and serum amyloid P (SAP) in species including but not limited to mouse, rat, rabbit, cat, dog, pig, cow, chicken, and monkey.. Data that illustrate the use of our kits to measure acute phase proteins in mouse ...
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F. Carrouel, M. J Staquet, J. F Keller, C. Baudouin, P. Msika, F. Bleicher, B. Alliot-Licht, and J. C Farges (2013) J Endod, 39(8):1008-14.. ...
In this study, we provide evidence based on both functional assays and structural modeling that is consistent with EsLBP1 functioning as an LBP-like protein. Most notably, EsLBP1 binds Gram-negative bacterial LOS and LPS with nanomolar or higher avidity under in vitro conditions, i.e., when LPS/LOS is presented as part of supramolecular assemblies containing LPS-rich lipid-water interfaces, as in aggregates of purified LPS/LOS. eslbp1 gene expression is regulated by exposure to the peptidoglycan monomer TCT, which synergizes with LPS in the triggering of V. fischeri-induced morphogenesis of the host symbiotic tissues. The gene is expressed and the protein produced across the organs epithelia-from the point where V. fischeri initially gathers, along the path of its migration, to where it takes up permanent residence in the crypts. The protein is also abundant along the apical surfaces of other epithelial tissues, where colonization by bacteria does not occur.. Although EsLBP1 has only ~25% ...
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Title: Inhalation exposure to nickel hydroxide nanoparticles induces systemic acute phase response in mice.. Authors: Kang, Gi Soo; Gillespie, Patricia Anne; Chen, Lung-Chi. Published In Toxicol Res, (2011 Mar). Abstract: It has been proposed that acute phase response can be a mechanism by which inhaled particles exert adverse effects on the cardiovascular system. Although some of the human acute phase proteins have been widely studied as biomarkers of systemic inflammation or cardiovascular diseases, there are only a few studies that investigated the role of serum amyloid P (SAP) , a major acute phase protein in mice. In this study, we investigated the changes in SAP, following inhalation exposure to nickel hydroxide nanoparticles (nano-NH) . We conducted 1) acute (4 h) exposure to nano-NH at 100, 500, and 1000 μg/m(3) and 2) sub-acute (4h/d for 3d) exposure at 1000 μg/m(3), then measured serum SAP protein levels along with hepatic Sap mRNA levels. The results show that inhaled nano-NH can ...
Acute phase protein Acute-phase proteins are a class of proteins whose plasma concentrations increase (positive acute phase proteins) or decrease (negative
The diet transition phase is thought to be the highest risk period for development of low ruminal pH, while pathology associated with low reticulo-ruminal pH (RRpH) induced ruminal acidosis (RA) is often found at slaughter, months after the diet transition. Two experiments were conducted to 1) determine the risk of low RRpH during the transition phase and 2) explore the association of rumen fermentation and acute phase protein response during finishing with pathology identified post mortem. In experiment 1, RRpH was measured in 32 mixed breed steers (n = 16) and heifers (n = 16) housed in commercial feedlot pens with 227 ±13 and 249 ± 6 hd/pen cohort steers and heifers, respectively. Cattle were transitioned from a diet containing 46.5% forage and 53.5% concentrate to a diet containing 9.5% forage and 90.5% concentrate dry matter (DM) basis) over 40 d. In addition, wheat replaced barley as the grain source during the dietary transition. Both mean and minimum RRpH decreased as the proportion of ...
Assessment of serum concentration of lipopolysaccharide (LPS)-binding protein (LBP) has been suggested as a useful biomarker to indicate activation of innate immune responses to microbial products. We investigated LBP concentrations and associations with demographics, lifestyle factors, and common metabolic abnormalities in adults. We also examined if LBP concentrations were associated with common polymorphisms in genes coding for LBP (rs2232618), CD14 (rs2569190), and TLR4 (rs4986790), the molecules responsible for the innate immune response to LPS, or serum levels of soluble CD14 (sCD14) and proinflammatory cytokines ...
Correction: 3LPS-binding protein and its interactions with P. gingivalis LPS modulate pro-inflammatory response and Toll-like receptor signaling in human oral keratinocytes. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Twenty patients with invasive cervical cancer of the squamous cell type were treated by radiotherapy and/or radical hysterectomy. During a follow-up period of approximately 2 yr in 10 of these patients a recurrence was established. Serial determinations of three acute phase reactants (α1-acid glycoprotein, haptoglobin and C-reactive protein) and of ... read more three complement components (C4, C3-proactivator and C9) were performed before and after therapy and either at the time of recurrence (recurrence group), or at the end of the follow-up period (non-recurrence group). Sequential analysis of the serum levels of all the parameters tested showed a significant increase at the time of recurrence. The most conclusive results were obtained with haptoglobin and the C9-component of complement. show less ...
The aim of this study was to investigate the effects of a housing period following long distance transport on haptoglobin (Hp), C-reactive protein (CRP) and pig major acute phase protein (pig-MAP) in swine. After transportation, 80 gilts were allotted to group A, B, C, or D. Blood samples were collected on arrival and 28 days later; additional samples were collected from Group C on day 14, and fro ...
Results : Body weight, % body fat, fasting glucose, total cholesterol, and NEFA were increased with WD and wheat had no effect on these metabolic parameters. Serum C reactive protein and lipopolysaccharide binding protein were not changed by WD or wheat. WD decreased the SCFA, acetic acid, but adding Gallagher wheat to WD restored levels to control (PWD*Wheat, 0.05). No other SCFA were altered. Histological evaluation revealed reduced villi height (P, 0.05) and area (P, 0.05) in the jejunum with WD and wheat did not alter this response. Within the ileum, Gallagher increased villi area (P, 0.01) relative to control, but no other changes were noted. No effects of WD or wheat on villous atrophy or lymphocyte infiltration within the jejunum, ileum or colon were observed. Overall, gene expression of tight junction proteins was unaffected by WD or wheat, except for a reduction in junction adhesion molecule-3 (Jam3) by WD (P, 0.05). Within the ilial lamina propria, WD increased interferon-γ (IFNg) (P, ...
IL-6 is produced by APCs such as macrophages, dendritic cells, and B cells, but also by nonhematopoietic cells (e.g., epithelial and endothelial cells, astrocytes, and fibroblasts) in response to external stimuli such as TNF-α, IL-1β, platelet-derived growth factor, or bacterial and fungal components. It binds to a receptor complex consisting of the specific IL-6Rα and the gp130 signal transducing unit that activates the transcription factor STAT3, among other signaling molecules. IL-6 is a pleiotropic cytokine that plays an important role in acute-phase protein synthesis, bone metabolism, central nervous system function, growth and drug response of tumors, and the immune response (for reviews see references 1, 2). Regarding its function in the immune system, IL-6 is now thought of as an important link between innate and adaptive immunity, mediating several aspects of B and T cell responses (3).. The role of IL-6 in CD4+ T cell function is multifaceted. IL-6 influences T cell effector ...
The proteasome as a lipopolysaccharide-binding protein in macrophages: differential effects of proteasome inhibition on lipopolysaccharide-induced signaling events ...
The host inflammatory response against infections is characterized by the release of pro-inflammatory cytokines and acute-phase proteins, driving both innate and adaptive arms of the immune response. Distinct patterns of circulating cytokines and acute-phase responses have proven indispensable for guiding the diagnosis and management of infectious diseases. This review discusses the profiles of acute-phase proteins and circulating cytokines encountered in viral and bacterial infections. We also propose a model in which the inflammatory response to viral (IL-18/ferritin) and bacterial (IL-6/CRP) infections presents with specific plasma patterns of immune biomarkers ...
Diabetes mellitus is one of public health problem in world. Type 2 diabetes millitus (T2DM) which resistance cell to insulin. more inflammation occur in Patients with T2DM because of elevation of inflammatory marker like C3, C4 & hsCRP. This study was aimed to detect the level of acute phase reactant proteins (C3 , C4 and hsCRP) in T2DM patients. Blood samples were collected from 60 patients with T2DM and control group was included 30 healthy donors. C3, C4 & hsCRP levels were estimated for all samples. Samples were grouped according to complications of patients. The results showed that there was a significant increase in C3 level for patients with T2DM (199.0 mg/dl) compared to control group (135.4 mg /dl) while C4 level for T2DM (27.25 mg/dl) showed no significant change compared to control group( 30.04mg/dl). C3 level of patients under heart disease (199.6 mg/dl ), patients under thyroid disease (230.4 mg/dl) and patients without any disease (188.1 mg/dl) recorded significant elevation
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Interleukin 1 receptor antagonist (IL-1Ra) is an acute-phase protein.: Interleukin 1 receptor antagonist (IL-1Ra) levels are elevated in the blood of patients w
Anogen is a Canadian biopharmaceutical company, who has been producing antibody products for research, diagnostic and therapeutic applications.
CD14 Antigens: Glycolipid-anchored membrane glycoproteins expressed on cells of the myelomonocyte lineage including monocytes, macrophages, and some granulocytes. They function as receptors for the complex of lipopolysaccharide (LPS) and LPS-binding protein.
Objective: We aimed to determine the potential value of maternal serum levels of acute phase reactants in the prediction of preterm delivery in women with ...
Simpson, R. J., Guy, K., Whyte, G. P., Middleton, N., Black, J. R., Ross, J. A., …Florida-James, G. D. (2006). Lymphocyte Phenotype Alterations, Pro-Inflammatory Cytokines and Acute Phase Proteins Following Repeated Bouts of Mountainous Hill-Running: 2278. Medicine and science in sports and exercise. 38(Supplement), S412???S413. doi:10.1249/00005768-200605001-01739. ISSN 0195-9131. ...
Simpson, R. J., Guy, K., Whyte, G. P., Middleton, N., Black, J. R., Ross, J. A., …Florida-James, G. D. (2006). Lymphocyte Phenotype Alterations, Pro-Inflammatory Cytokines and Acute Phase Proteins Following Repeated Bouts of Mountainous Hill-Running: 2278. Medicine and science in sports and exercise. 38(Supplement), S412???S413. doi:10.1249/00005768-200605001-01739. ISSN 0195-9131. ...
Heatmap depicting circulating microbial products, acute phase proteins and inflammatory cytokines in CP Ag+ individuals compared to EN, INF and CP Ag− individ
SPR reveals ColN‐R is responsible for LPS binding. Histidine‐tagged ColN domain combinations (500 nM) were injected for 60 s at a flow rate of 5 μl m
Diagnosis of acute kidney injury (AKI) relies on a late marker, namely serum creatinine (SCr). New biomarkers are considered for early and sensitive detection of CIN. In particular, uNGAL has been used for early detection of AKI in the emergency department, after cardiopulmonary bypass or following CM administration.. This study will be conducted to assess the possible value of urinary Neutrophil Gelatinase-Associated Lipocalin (uNGAL) as an early detector of contrast-induced nephropathy (CIN) in a large sized cohort of patients undergoing percutaneous coronary procedures (PCP) and whether or not uNGAL correlates with the volume of contrast medium (CM) used. ...
TY - JOUR. T1 - Neutrophil Gelatinase-Associated Lipocalin Is Not Associated with Tacrolimus-Induced Acute Kidney Injury in Liver Transplant Patients Who Received Mycophenolate Mofetil with Delayed Introduction of Tacrolimus. AU - Fukuda, Mio. AU - Suetsugu, Kimitaka. AU - Tajima, Soichiro. AU - Katsube, Yurie. AU - Watanabe, Hiroyuki. AU - Harada, Noboru. AU - Yoshizumi, Tomoharu. AU - Egashira, Nobuaki. AU - Mori, Masaki. AU - Masuda, Satohiro. PY - 2019/6/25. Y1 - 2019/6/25. N2 - Tacrolimus is widely used as an immunosuppressant in liver transplantation, and tacrolimus-induced acute kidney injury (AKI) is a serious complication. The urinary neutrophil gelatinase-associated lipocalin (NGAL) level has been linked to tacrolimus-induced AKI in patients starting tacrolimus treatment the morning after liver transplantation. Here we tested this association using a different immunosuppression protocol: Mycophenolate mofetil administration beginning on Postoperative Day 1 and tacrolimus administration ...
Different molecular forms of urinary neutrophil gelatinase-associated lipocalin (NGAL) have recently been discovered. We aimed to explore the nature, source and discriminatory value of urinary NGAL in
García, T., Betbesé, A., García, M. et al. Erratum to: Urinary neutrophil gelatinase-associated lipocalin as an early predictor of acute kidney injury in cardiac surgery patients. Crit Care 17, P546 (2013). Download ...
Acute kidney injury (AKI) is a devastating potential consequence of renal ischaemia and reperfusion (I-R) subsequent to severe intra-operative hypotension and fluid resuscitation. Acute tubular epithelial damage is a common early histological abnormality in this syndrome. The high mortality rate associated with AKI in dogs is attributed in part to the limitations of current diagnostic techniques that can only detect AKI in the late stages when damage is irreversible. Early detection of renal tubular injury could improve outcome and might be possible by measuring urinary neutrophil gelatinase-associated lipocalin concentration (uNGAL) in at-risk dogs.. The objectives of this study were to establish a clinically relevant canine model of renal I-R injury, and use this model to determine changes in uNGAL within three hours of initiation of injury.. A pilot study was performed to establish the severity and duration of hypotension caused by haemorrhage, and duration of reperfusion, that produced ...
Neutrophil gelatinase-associated lipocalin has been used for the diagnosis, prognosis and severity assessment of AKI and has been validated in paediatric populations (where comorbidity is low) and in conditions where the timing of the insult is clear (that is cardiac surgery and so on) [20-24]. Its role in an adult ICU population has not been well validated due to the heterogeneity and uncertainty of the timing of the insult. The pathophysiology and causes of AKI in the ICU could be indigenous [25] and may differ from pre-ICU causes (low-volume state, inotropes, contrast injury and so on). This has led to problems in the design and interpretation of studies in the general adult ICU patient cohort. In this study, we sought to overcome these problems by excluding patients with pre-existing chronic kidney disease (CKD) and/or AKI and by looking at the predictive value of both urinary and plasma NGAL at different time points following admission.. We found the incidence of AKI was 30.4% (n = 59) with ...
TY - JOUR. T1 - Neutrophil gelatinase-associated lipocalin regulates gut microbiota of mice. AU - Mori, Katsuya. AU - Suzuki, Takeshi. AU - Minamishima, Shizuka. AU - Igarashi, Toru. AU - Inoue, Kei. AU - Nishimura, Daisuke. AU - Seki, Hiroyuki. AU - Yamada, Takashige. AU - Kosugi, Shizuko. AU - Katori, Nobuyuki. AU - Hashiguchi, Saori. AU - Morisaki, Hiroshi. PY - 2016/1/1. Y1 - 2016/1/1. N2 - Background and Aim: Because neutrophil gelatinase-associated lipocalin (NGAL) is known to provide significant bacteriostatic effects during infectious conditions, we tested the hypothesis that this protein is up-regulated and secreted into the intraluminal cavity of the gut under critically ill conditions and is thus responsible for the regulation of bacterial overgrowth. Methods: With our institutional approval, male C57BL/6J mouse (6-7weeks) were enrolled and applied for lipopolysaccharide or peritonitis model compared with naïve control. We assessed NGAL protein concentrations in intestinal lumen and ...
Neutrophil gelatinase-associated lipocalin (NGAL) is a protein expressed by kidney tubular cells in response to ischemia, but may also be an early indicator of immunological rejection, calcineurin inhibitor toxicity, obstructive nephropathy, subclinical tubulitis or infection. Although there is currently no evidence to support the routine serial measurement of blood or urinary NGAL to detect subclinical acute tubular injury, NGAL has the potential to provide useful information to those that care for kidney transplant recipients (KTRs). First, high urinary or serum NGAL concentrations shortly after transplantation are a predictor of delayed graft function and are associated with reduced graft function at one year. Secondly, among KTRs with previously stable graft function who then suffer acute graft dysfunction, a high urinary NGAL predicts graft loss at one year. If further refined, diagnostic tests based on NGAL levels may provide future useful clinical tools.
Neutrophil gelatinase-associated lipocalin (NGAL) is a small 25-kDa protein released from kidney tubular cells after harmful stimuli. It represents one of the most promising future biomarkers in the diagnostic field of acute kidney injury (AKI), as the increase in NGAL levels is a good predictor of a brief-term onset of AKI, notably anticipating the resulting increase in serum creatinine. However, recent studies also suggest a possible role for NGAL in chronic kidney disease (CKD). For this reason we evaluated serum (sNGAL) and urinary NGAL (uNGAL) in a cohort of CKD patients in order to verify the relationship with the severity of renal impairment. In CKD patients sNGAL, uNGAL and the fractional excretion of this protein were notably increased as compared to controls. Furthermore both sNGAL and uNGAL were correlated with serum creatinine and, inversely, with residual glomerular filtration rate (GFR): this last relationship was found to be even closer than that found between GFR and serum ...
Lipocalin-2, human recombinant protein, Neutrophil gelatinase-associated lipocalin, NGAL, p25, 25 kDa alpha-2-microglobulin-related subunit validated in (PBV10492r-10), Abgent
Evaluation of Neutrophil Gelatinase-Associated Lipocalin and Cystatin C in Early Diagnosis of Chronic Kidney Disease in the Absence of the Gold Standard
McLean , M H , Thomson , A J , Murray , G I , Fyfe , N , Hold , G L & El-Omar , E M 2013 , Expression of neutrophil gelatinase-associated lipocalin in colorectal neoplastic progression : a marker of malignant potential? , British Journal of Cancer , vol. 108 , no. 12 , pp. 2537-2541 . ...
Nephropathia epidemica (NE) is a mild form of hemorrhagic fever with renal syndrome (HFRS) that is caused by the Puumala virus. Periodic outbreaks have been described in endemic areas, with a substantial number of previously healthy individuals developing acute kidney injury (AKI). There is a considerable diversity in the clinical course of the disease, and few patients require renal replacement therapy. We tested whether urinary neutrophil gelatinase associated lipocalin (uNGAL), urine albumin/creatinine ratio (uACR), urine protein/creatinine ratio (uPCR), urine dipstick protein, C-reactive protein, procalcitonin, leukocyte and platelet count, determined on admission to the hospital, can predict the severity of AKI. Sixty-one patients were analyzed during admission in the emergency department. The variables most strongly associated with peak plasma creatinine concentration were uNGAL (β = 0.70, p |0.0001), uPCR (β = 0.64, p = 0.001), uACR (β = 0.61, p = 0.002), and dipstick proteinuria (β = 0.34, p
Abstract Elderly is the main age group affected by acute kidney injury (AKI). There are no studies that investigated the predictive properties of urinary (u) NGAL as an AKI marker in septic elderly population. This study aimed to evaluate the efficacy of uNGAL as predictor of AKI diagnosis and prognosis in elderly septic patients admitted to ICUs. We prospectively studied elderly patients with sepsis admitted to ICUs from October 2014 to November 2015. Assessment of renal function was performed daily by serum creatinine and urine output. The level of uNGAL was performed within the first 48 hours of the diagnosis of sepsis (NGAL1) and between 48 and 96 hours (NGAL2). The results were presented using descriptive statistics and area under the receiver operating characteristic curve (AUC-ROC) and p value was 5%. Seventy-five patients were included, 47 (62.7%) developed AKI. At logistic regression, chronic kidney disease and low mean blood pressure at admission were identified as factors associated ...
One-hundred and eighty-one patients (66.1%) were men; mean age was 68.2 ± 12.2 years. Valve replacement was performed in 123, coronary artery bypass graft (CABG) in 81, valve surgery + CABG in 48, cardiac transplant in five, aorta aneurism surgery in nine, and other procedures in eight patients. ICU and hospital stays were 6.7 ± 8.1 and 15.7 ± 13.9 days, respectively. Renal replacement therapy (RRT) was required in 16 patients (5.8%) within 48 hours of ICU stay and in 28 patients (10.2%) within 43weeks. Mortality at 28 days was 2.9%. Eighty-six patients (31.4%) were diagnosed with AKI within 48 hours of surgery. Area under the ROC curve of POST uNGAL for AKI diagnosis was 0.72 (0.66 to 0.79) (P 0.0001) at an optimal cutoff value of 1803 μg/l, with 78.7% specificity, 64% sensitivity and 74.1% accuracy. uNGAL advanced diagnosis of AKI in 44 patients (51.2%), whereas diagnosis was achieved at the same time as AKI criteria in 11 patients; AKI criteria outperformed uNGAL in only 36% of cases. ...
AKI occurs frequently in the hospital setting and affects morbidity, mortality, and resource use, regardless of country, etiology, setting, or definition. In current practice, AKI is identified by a large, persistent increase in serum creatinine levels. However, physicians want to diagnose AKI as early as possible, and the serum creatinine increase happens days after AKI occurs. Early changes in serum creatinine are not particularly ...
Semantic Scholar extracted view of Neutrophil gelatinase-associated lipocalin as an early indicator for postoperative renal failure by CD Van der Marel et al.
敗血症(Sepsis)是造成急性腎損傷(Acute kidney injury, AKI)的主因,而AKI又為敗血症患者死因之一,研究認為NGAL(Neutrophil gelatinase-associated lipocalin)可作為預測AKI的生物標誌,但因NGAL又為感染及全身性發炎的生物標誌,可能受多重因素干擾。目前AKI患者可依RIFLE(Risk、Injury、Failure、Loss of kidney function及End-stage kidney disease)分級,若腎臟代替療法(Renal replacement therapy)於RIFLE-Failure(F)期之前進行,可提升治療結果,但AKI患者接受腎臟代替療法時機仍未有共識,因此發表於《Journal of the Formosan Medical Association》的最新文章評估血漿中NGAL及細胞激素是否可作為預測RIFLE-F及啟動腎臟代替療法的生物標誌。試驗中,30位健康受試者及85位患者接受採血,分析肌酸酐(creatinine, ...
A lateral flow device comprised of a strip of porous nitrocellulose is coated near its distal end with a capture antibody against NGAL applied as a transverse band. A further transverse band of antibody against antibodies of the species from which the detection antibody is derived is placed distally to the capture antibody band and serves as a control of strip function. The proximal end of the strip contains the detection antibody against NGAL adsorbed or linked to labeled polystyrene particles or particles of dye complex. When an aliquot of the centrifuged urine sample is applied to the proximal end of the strip, the labeled particles attached to detection antibody travel along the strip by capillary attraction. When reaching the band of capture antibody, only those particles which have bound NGAL will be retained, giving rise to a detectable band. Particles reaching the control band of antibody against the detection antibody will produce a detectable band whether or not any NGAL has been ...
Nøytrofil gelatinase-assosiert lipocalin i inflammatorisk tarmsykdom Inflammatorisk tarmsykdom (IBD) er kroniske betennelsestilstander i mage-tarmsystemet. De to hovedformene er Crohns sykdom (CD) og ulcerøs kolitt (UC). Årsaken til sykdommene er ukjent. Sykdomsmekanismene er komplekse og involverer blant annet genetiske faktorer, komponenter av det medfødte og ervervede immunforsvaret og tarmens mikrobeflora. Eksisterende behandling er utilfredsstillende. Mye forskning har blitt utført for å forstå sykdomsmekanismene bedre, for å finne bedre diagnostiske verktøy og bedret behandling. Vår forskningsgruppe har tidligere utført analyse av genregulering i tykktarmsslimhinne der det ble funnet at genet LCN2 var blant de mest oppregulerte genene ved aktiv betennelse sammenlignet med kontroller. Proteinet som kodes av LCN2, nøytrofil gelatinase-assosiert lipocalin (NGAL), var høyt uttrykt i en klasse av hvite blodlegemer og i betente overflateceller (epitel). NGAL er kjent som et ...
Looking for online definition of Acute phase reactant in the Medical Dictionary? Acute phase reactant explanation free. What is Acute phase reactant? Meaning of Acute phase reactant medical term. What does Acute phase reactant mean?
Subramanian Senthilkumaran, Ponniah Thirumalaikolundusubramanian, Namasivayam Elangovan Journal of Emergencies, Trauma, and Shock 2019 12(4):260-262 Backgrou
Neutrophil gelatinase-associated lipocalin (NGAL), an acute phase protein released by neutrophils, has been described as biomarker of inflammatory states. Type 2 diabetes mellitus (T2DM) is characterized by increased inflammation and an elevated risk for embolization of carotid artery stenosis (CAS). We aimed to explore the role of NGAL systemically and in plaques of diabetics undergoing carotid endarterectomy. Moreover, the potential anti-inflammatory effect of metformin on NGAL was addressed in diabetics. Serum NGAL and matrix metalloproteinase (MMP)-9/NGAL levels were measured in 136 patients (67 with T2DM vs. 69 non-diabetics) by specific ELISA. Endarterectomy samples were graded histologically according to the American Heart Association´s classification. NGAL mRNA expression was detected using RealTime-PCR in carotid endarterectomy specimens. Serum NGAL [median 107.4 ng/ml (quartiles: 75.2-145.0) vs. 64.4 (50.4 -81.3), p | 0.0001] and MMP-9/NGAL [41.5 ng/ml (20.8-63.9) vs. 27.6 (16.0-42.4), p = 0
Background and aims the role of serum neutrophil gelatinase-associated lipocalin (NGAL) in predicting delayed graft function (DGF) after kidney transplantation is poorly defined. The objective of this study was to evaluate the serum NGAL expression in the early postoperative phase after kidney...
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The neutrophilic granulocyte is a cytotoxic and potentially tissue-injuring cell participating in the destructive processes and symptoms seen in a variety of inflammatory diseases. Sensitive immunoassays have been introduced to measure the levels of specific secretory proteins of various inflammatory cells in blood and other body fluids. The aim has been to develop highly specific markers for each cell type. The results obtained by immunoassay have indicated that human neutrophil lipocalin (HNL) is a protein unique to the neutrophil. The present study investigated the specificity of HNL as a neutrophil marker in peripheral blood and lung tissue by using flow cytometry and immunocytochemistry. Flow cytometry and immunocytochemistry on peripheral blood showed that monoclonal antibodies to HNL only react with neutrophils and not with other types of leukocytes. Immunocytochemistry on plastic-embedded sections and on frozen sections of lung tissue showed that a cocktail of six monoclonal antibodies ...
The human neutrophil lipocalin (HNL), a member of the large family of lipocalins that exhibit various physiological functions, is coexpressed in granulocytes with progelatinase B (MMP-9). Part of it is covalently bound to the proenzyme and therefore may play a possible role in the activation process: of promatrix metalloproteinases. We now report that HNL is able to accelerate the direct activation of promatrix metalloproteinases slightly. A significant enhancement of the activity could be demonstrated for the HgCl2- and the plasma kallikrein-induced activation of all three secretory forms of proMMP-9 and of proMMP-8. The same activating effects were exerted by HNL isolated from granulocytes as well as by the recombinant forms expressed by the yeast Pichia pastoris or by Escherichia coli. This demonstrates that the carbohydrate moiety is not essential for the biological activity of HNL. Activation and activity enhancement are obviously mediated by entrapping the remaining N-terminal sequence ...
This PhD sought to exploit the acute effects of exercise upon the kidneys to make tenable links to pathological states such as acute kidney injury (AKI) and chronic kidney disease (CKD). It is surprising that such associations with their potential clinical implications have received limited attention so far despite the ever-increasing number of healthy individuals participating in vigorous and physiologically challenging activities. The work herein has shown how experimental in-vivo exercise models may be used to simulate a stressed kidney with features that resemble diseased states. Summarising the key findings briefly, the first study (chapter 2) demonstrated that maximal-intensity exercise in the form of an 800 metre sprint resulted in increased urinary concentrations of an AKI biomarker (neutrophil gelatinase-associated lipocalin / NGAL), suggesting mild kidney stress or a concentrating effect. However, plasma NGAL concentrations decreased and urinary rises were independent of ...
The aim of this study was to investigate the interrelationships between three bone-derived factors [serum osteocalcin (OCN), fibroblast growth factor (FGF) 23, and neutrophil gelatinase-associated lipocalin (NGAL) levels] and body fat content and distribution, in order to reveal the potential endocrine function of bone in the development of obesity. We recruited 1179 people (aged 59.5 ± 6.2 years) from communities in Shanghai. Serum OCN levels were determined using an electrochemiluminescence immunoassay. Serum FGF23 and NGAL levels were determined using a sandwich enzyme-linked immunosorbent assay. The abdominal fat distribution, including visceral fat area (VFA), was assessed by magnetic resonance imaging. Visceral obesity was defined as a VFA ≥ 80 cm2. Serum OCN levels were inversely correlated with body fat parameters, while FGF23 and NGAL were positively correlated (P | 0.05). After adjusting for confounders, waist circumference (W) and VFA had a closer relationship with serum OCN, FGF23, and
Results There were 42 female and 31 male patients with an age range of 18-54 years. In the comparison of laboratory parameters of the patients with or without the components of BDCAF, no significant relation could be found between headache, erythema nodosum, superficial thrombophlebitis, skin pustules, GIS and eye involvement and acute phase response parameters. However, we found significantly higher levels of ferritin in patients with arthritis and higher levels of fibrinogen in patients both with arthralgia and arthritis versus without joint involvement (p=0,01; p=0,030 and p=0,000; respectively). Also Hp levels in patients with genital ulceration and arthritis (p=0,005 and p=0,002) and Cp levels in patients with oral ulceration and arthritis were found to be higher compared to patients without these involvements (p=0,01 and p=0,001). Prealbumin as a negative acute phase reactant was determined as significantly lower in patients with joint involvement than the patients without it (p=0,023). ...
Kaori Maeda, Luke O. Schoeniger, Masahiko Shimada, Richard A. Winchurch, Timothy G. Buchman, James L. Robotham; Regulation of Acute Phase Gene Expression Following Surgery and Endotoxin Administration in the Anesthetized Pig. Anesthesiology 1993;79(6):1324-1337. Download citation file:. ...
For the kidney allograft assessment will be used measurements performed during machine perfusion in hypothermia: renal flow, resistance, lactate dehydrogenase, lactates and ischemia injury markers measured in the fourth hour of perfusion in perfusion fluid.. For kidney ischemia injury assessment such markers will be measured: tumour necrosis factor (TNF alfa), interleukin 2 (IL-2), interleukin 6 (IL-6), high sensitivity C-reactive protein (hsCRP), platelet-derived growth factor (PDGF), cystatin C, kidney Injury Molecule (KIM-1), neutrophil Gelatinase-associated Lipocalin (NGAL), complement component C3, caspase 3.. Every time from pair of retrieved kidneys each kidney will be randomise for one of the group:. ...
BACKGROUND: Several biomarkers are becoming available for the early detection of acute kidney injury (AKI), but few have been directly compared.. OBJECTIVE: To compare urinary kidney injury molecule-1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL), and N-acetyl glucosaminidase (NAG) against serum creatinine and renal histological score in the initiation, maintenance, and recovery phases of cisplatin (CP)-induced AKI.. METHODS: Sprague-Dawley rats (300-350 g) were injected once through their tail veins with CP (CP group) at 5.5 mg/kg or with same volume of normal saline vehicle (Control group). Rats were euthanized at 2, 4, 6, 12, and 24 hours, and on days 2, 3, 6, and 10 (n = 12 in the CP group and n = 6 in the Control group at each time point), and urine, blood, and kidney samples were analyzed.. RESULTS: A significant increase in serum creatinine was noted by day 3 in the CP group versus Control group [1.46 (0.12) vs 0.28 (0.03) mg/dL; mean (SE); P , 0.05]. The renal histology ...
Mechanisms of progression of chronic kidney disease (CKD), a major health care burden, are poorly understood. EGFR stimulates CKD progression, but the molecular networks that mediate its biological effects remain unknown. We recently showed that the severity of renal lesions after nephron reduction varied substantially among mouse strains and required activation of EGFR. Here, we utilized two mouse strains that react differently to nephron reduction - FVB/N mice, which develop severe renal lesions, and B6D2F1 mice, which are resistant to early deterioration - coupled with genome-wide expression to elucidate the molecular nature of CKD progression. Our results showed that lipocalin 2 (Lcn2, also known as neutrophil gelatinase-associated lipocalin [NGAL]), the most highly upregulated gene in the FVB/N strain, was not simply a marker of renal lesions, but an active player in disease progression. In fact, the severity of renal lesions was dramatically reduced in Lcn2-/- mice. We discovered that Lcn2 ...
Although the repression of inflammatory cytokines by glucocorticoids is well studied as being the basis of the anti-inflammatory action of glucocorticoids the effect of inflammatory cytokines on glucocorticoid action, especially in the periphery, is less well studied. Having shown in vivo that IL-6 antagonizes the effect of glucocorticoids in the liver we expanded the study in tissue culture and found that glucocorticoids and pro-inflammatory cytokines generally have divergent effects on the GR levels and metabolic enzymes, while their functions are convergent on the acute phase proteins. Further work on the interaction between the stress and inflammatory system is focussing on corticosteroid binding globulin (CBG), which is both a negative acute phase protein synthesised in the liver and the plasma binding protein involved in the transport of glucocorticoids. ...
Radcliffe Cardiology article authored by Niall G Mahon covering topics - Neutrophil gelatinase-associated lipocalin, acute kidney injury, cardiorenal syndrome & on other cardiology field
Background.The role of serum cystatin C (Scyc), neutrophil gelatinase-associated lipocalin, and interleukin-18 in predicting early graft function after kidney transplant is poorly defined.Methods.We conducted a multicenter prospective cohort study of deceased-donor kidney transplants. We collected s
Aims: Lactate is produced by anaerobic metabolism and may reflect inadequate tissue perfusion in conditions such as acute heart failure (AHF). We evaluated the prevalence and clinical significance of elevated blood lactate on admission in patients with AHF. Methods and results: We enrolled 237 patients with AHF (mean age 67 ± 12 years; 70% men) presenting without overt clinical evidence of peripheral hypoperfusion (warm haemodynamic profile). Median (upper and lower quartiles) blood lactate on admission was 1.8 (1.5; 2.4) mmol/L; 103 (43%) patients had an elevated blood lactate (≥2 mmol/L). Patients with an elevated lactate had higher blood high-sensitivity troponin I [15.4 (8.5; 26.1) vs. 9.9 (4.3; 19.6) pg/mL], aspartate aminotransferase [28 (20; 44) vs 24 (19; 36) IU/L] and endothelin-1 (12.1 ± 6.2 vs. 9.3 ± 3.9 pg/mL) (all P , 0.05). In this group plasma concentration of neutrophil gelatinase-associated lipocalin increased during the first 48 h, whereas values fell for those with ...
Health, ...EDITORS PICK: How the drug isotretinoin zaps acne ...The most potent drug available for the treatment of acne is 13-cis ret...Initial analysis confirmed previous observations made using cultured c...TITLE: Neutrophil gelatinase-associated lipocalin mediates 13-ci...,JCI,online,early,table,of,contents:,March,3,,2008,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
Mellor, A and Boos, C and Stacey, M and Hooper, T and Smith, C and Begley, J and Yarker, J and Piper, R and OHara, J and King, R and Turner, S and Woods, DR (2013) Neutrophil gelatinase-associated lipocalin: its response to hypoxia and association with acute mountain sickness. Disease markers, 35 (5). 537 - 542. ISSN 0278-0240 DOI: ...
TY - JOUR. T1 - Modulation of types I and II acute phase reactants with insulin-like growth factor-1/binding protein-3 complex in severely burned children. AU - Spies, Marcus. AU - Wolf, Steven E.. AU - Barrow, Robert E.. AU - Jeschke, Marc G.. AU - Herndon, David N.. PY - 2002/1/1. Y1 - 2002/1/1. N2 - Objective: To determine whether 0.5 mg/kg insulin-like growth factor (IGF)-1/binding protein (IGFBP)-3, given intravenously, effectively alters the acute phase response in severely burned children. Design: Longitudinal trial with each patient serving as their own control. Setting: University-affiliated pediatric burn center. Patients: Nine children, 15 yrs of age or less, with burns covering ,40% of the total body surface area. Interventions: Standard burn care with early burn wound excision and grafting. Blood sampled at defined time points before and after operative procedures. Measurements and Results: Determination of types I and II acute phase reactant proteins, constitutive serum proteins, ...
In 81% of the critically ill patients with polytrauma the post-traumatic period was accompanied with development of infectious complications, Gram-negative (K. pneumoniae, Acinetobacter spp., E. coli) and Gram-positive (S. Epidermidis, S. aureus). Sepsis was diagnosed on 8 to 10 days in 45% of the patients. The significant increase of LPS-BP was found in the first 3 days of the follow-up, compared with the control values (6.7 times higher in SIRS group (χ2(n = 18, df = 3) = 52.8666, P , 0.001); 9.9 times higher in the group with local infection (χ2(n = 36, df = 3) = 91.6629, P , 0.001); 15.2 times higher in the sepsis group; 20.5 times higher in the severe sepsis group; 47.3 times higher in the septic shock group (χ2(n = 6, df = 3) = 11.0339, P = 0.0115)), whereas the first positive results of the microbiological examination were obtained only on 5 to 7 days. The diagnostic sensitivity of threshold concentration of LBP in blood serum (335 mkg/ml) was 84%, diagnostic specificity was 88% (ROC ...
The serum protein 1-acid glycoprotein (AGP), also known as orosomucoid, is normally referred to as an archetypical positive acute phase protein. as analyzed by 2-D electrophoresis. This MAb was utilized to build up an immunoassay (ELISA) for quantification of AGP in pig serum samples. The adult serum concentrations of pig AGP had been in the number of 1C3 mg/ml in several regular pig breeds although it was low in G?ttingen and Ossabaw minipigs (in the 0.3 to 0.6 mg/ml vary) and higher in young (2C5 times old) regular pigs (mean: 6.6 mg/ml). Amazingly, pig AGP was discovered to Betanin work as a poor acute phase proteins during a selection of experimental infections and aseptic irritation with significant decreases in serum focus and in hepatic ORM1 expression through the acute stage response. To your understanding this is actually the first explanation in virtually any species of AGP being truly a negative severe phase protein. Launch Alpha-1-acid glycoprotein (AGP), also referred to as ...
C-reactive protein (CRP) is an acute-phase reactant frequently used in histochemistry like a marker of ongoing inflammation. [AA] 218 to 232) was identified by CP-724714 monoclonal antibodies CRP-8 and 9C9. This epitope region of Hsp60 displays 26.6% amino acid identity to CRP AA region 77 to 90. These data suggest that the B-cell epitopes shared between CRP and Hsp60 give rise to a true mimicry-based cross-reaction and the induction of cross-reactive antibodies. Our study underlines the importance of thorough study design and careful interpretation of results while using polyclonal anti-CRP antibodies for histochemistry, especially at low dilutions. Furthermore, analytical interference with Hsp60 in CRP assays should also become tested. C-reactive protein (CRP) is an acute-phase reactant in humans, rabbits, and a number of additional mammalian varieties. It is indicated and secreted primarily by hepatocytes, but recently, local production at sites of swelling by monocytes has also been reported ...
Epidemiological studies link inhalation of particles to increased risk of cardiovascular disease. Inhaled particles may induce cardiovascular disease by several different mechanisms including translocation of particles to systemic circulation, activation of airway sensory nerves resulting in autonomic imbalance and particle-induced pulmonary inflammation and acute phase response. The acute phase response is the systemic response to acute and chronic inflammatory states caused by for example bacterial infection, virus infection, trauma and infarction. It is characterized by differential expression of ca. 50 different acute phase proteins including C-reactive protein and Serum amyloid A, which are the most differentially up-regulated acute phase response proteins. Blood levels of these two acute phase proteins are closely associated with risk of cardiovascular disease in epidemiological studies and SAA has been causally related to the formation of plaques in the aorta in animal studies. In a recent paper
A method of determining the proliferative status of a carcinoma is disclosed. One obtains a patient sample and then quantitatively analyzes the sample for NGAL gene expression product. The amount of NGAL expression product is compared with a standard curve to determine the S-phase value. The sample can be breast tissue or breast fluid aspirate. Alternatively, blood can by analyzed for this marker to diagnose metastasis.
Acute kidney injury (AKI) is a complex, frequent and serious clinical problem with high rate of mortality. Therefore there is a serious need for early detection of AKI, with a tendency to detect early stage--RISK dut to start with therapy as soon as possible and prevent irreversible changes in renal function. Studys purposes were to explore the rhythm of urine neutrophile gelatinase associated lipocalin (urine NGAL) concentration changes before and after cardiovascular surgery and compare results of urine NGAL values with results of serum creatinine and creatinine clearance as main diagnostic indicators of renal function in order to define role of urine NGAL biomarker in early diagnosis of acute kidney injure ...
Recognition of bacterial lipopolysaccharide (LPS) by the innate immune system elicits strong pro-inflammatory responses that can eventually cause a fatal sepsis syndrome in humans. LPS-mediated activation of mammalian cells is believed to involve the interaction of LPS with lipopolysaccharide-binding protein (LBP) in the serum and, subsequently with CD14. Although there is no doubt that CD14 binds LPS, CD14 is not capable of initiating a transmembrane activation signal because it is a glycosylphosphatidylinositol (GPI)-anchored protein. Accumulating evidence has suggested that LPS must interact with a transmembrane receptor(s) that is responsible for signal transduction. Integrins CD11c and/or CD18, Toll-like receptors (TLRs), as well as CD55, have been suggested to serve this function. Recently, we have revealed that a signalling complex of receptors is formed following LPS stimulation, which comprises heat-shock proteins (Hsps) 70 and 90, chemokine receptor 4 (CXCR4) and growth differentiation ...
... in contrast to other acute phase proteins, e.g., C-reactive protein, which increase in case of acute inflammation). ... Transferrin is an acute phase protein and is seen to decrease in inflammation, cancers, and certain diseases ( ... Jain S, Gautam V, Naseem S (January 2011). "Acute-phase proteins: As diagnostic tool". Journal of Pharmacy & Bioallied Sciences ... "Reference distributions for the negative acute-phase serum proteins, albumin, transferrin and transthyretin: a practical, ...
Mary R, Veinberg F, Couderc R (2003). "[Acute meningitidis, acute phase proteins and procalcitonin]". Annales de Biologie ... "Assessment of the prognostic value of certain acute-phase proteins and procalcitonin in the prognosis of acute pancreatitis". ... It is therefore often classed as an acute phase reactant. The induction period for procalcitonin ranges from 4-12 hours with a ... PCT serves a marker to help differentiate acute respiratory illness such as infection from an acute cardiovascular concern. It ...
Acute phase proteins are markers of inflammation. Autoantibodies are usually absent or very low, so instead of being given in ... "C-reactive protein". GPnotebook. 2730 Serum C-Reactive Protein values in Diabetics with Periodontal Disease Archived 2008-12-20 ... Derived from mass values using molar mass of 314.46 g/mol Bhattacharya Sudhindra Mohan (July/August 2005) Mid-luteal phase ... Included here are also related binding proteins, like ferritin and transferrin for iron, and ceruloplasmin for copper. Note: ...
"Amyloidosis Overview". Centre for Amyloidosis and Acute Phase Proteins. University College London. 22 May 2018. Retrieved 27 ...
Genes on human chromosome 12, Acute-phase proteins). ... α2-macroglobulin may act as a carrier protein because it also ... The amino acid sequence of α2-macroglobulin has been shown to be 71% the same as that of the pregnancy zone protein (PZP; also ... α2-Macroglobulin (α2M), or alpha-2-macroglobulin, is a large (720 KDa) plasma protein found in the blood. It is mainly produced ... Dodds AW, Law SK (December 1998). "The phylogeny and evolution of the thioester bond-containing proteins C3, C4 and alpha 2- ...
... (SAA1) is a protein that in humans is encoded by the SAA1 gene. SAA1 is a major acute-phase protein mainly ... Zimlichman S, Danon A, Nathan I, Mozes G, Shainkin-Kestenbaum R (Aug 1990). "Serum amyloid A, an acute phase protein, inhibits ... "Entrez Gene: SAA1 serum amyloid A1". Gabay C, Kushner I (Feb 1999). "Acute-phase proteins and other systemic responses to ... Malle E, Sodin-Semrl S, Kovacevic A (Jan 2009). "Serum amyloid A: an acute-phase protein involved in tumour pathogenesis". ...
Response elements to fatty acids, acute phase proteins, serum. and to the immune factor NF-κB were also observed. The presence ... It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein ... Protein Engineering. 10 (6): 621-5. doi:10.1093/protein/10.6.621. PMID 9278274. Zeng C, Spielman AI, Vowels BR, Leyden JJ, ... ApoD mRNA and protein increases in the ipsilateral region of hippocampus as early as 2 days post-lesion (DPL), remains high for ...
OSM can regulate the expression of acute phase proteins. OSM regulates the expression of various protease and protease ... Of the proteins recruited to type I cytokine receptors STAT proteins remain the best studied. Homodimerisation of gp130 has ... "Differential activation of acute phase response factor/STAT3 and STAT1 via the cytoplasmic domain of the interleukin 6 signal ... effect on the acute phase reaction". Z Ernahrungswiss. 37 Suppl 1: 43-9. PMID 9558728. Schieven GL, Kallestad JC, Brown TJ, ...
Human SAP has 51% sequence homology with C-reactive protein (CRP), a classical acute phase response plasma protein, and is a ... Genes on human chromosome 1, Blood proteins, Acute-phase proteins). ... The serum amyloid P component (SAP) is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein first ... This association is utilised in the routine clinical diagnostic technique of SAP scintigraphy whereby radio-labelled protein is ...
Many acute-phase proteins of inflammation are involved in the coagulation system. In addition, pathogenic bacteria may secrete ... Thrombomodulin binds these proteins in such a way that it activates Protein C. The activated form, along with protein S and a ... as well as Protein S, Protein C and Protein Z. In adding the gamma-carboxyl group to glutamate residues on the immature ... Protein C is activated in a sequence that starts with Protein C and thrombin binding to a cell surface protein thrombomodulin. ...
Disulfide groups stabilize the tertiary structures of proteins. Transferrins are iron binding proteins and acute phase ... Protein articles without symbol, Blood proteins, Storage proteins, Avian proteins). ... Consequently, structurally this protein differs from its serum counterpart because of its glycosylation pattern. These proteins ... Egg white albumen is composed of multiple proteins, of which ovotransferrin is the most heat reliable. It has a molecular ...
... diarrhoea and acute phase proteins in naturally infected dairy calves". Comp Immunol Microbiol Infect Dis. 41: 10-6. doi: ...
Heavy infections with Eimeria zuernii in calves can produce more of the acute phase proteins haptoglobin and serum amyloid A ... Lassen, B.; Bangoura, B.; Lepik, T.; Orro, T. (2015). "Systemic acute phase proteins response in calves experimentally infected ... diarrhoea and acute phase proteins in naturally infected dairy calves". Comp Immunol Microbiol Infect Dis. 41: 10-6. doi: ...
This test utilises the acute phase proteins (C-Reactive Protein and Haptoglobin). In combination with basic clinical symptoms, ... Serum thymadine kinase 1 and C-reactive protein as biomarkers for screening clinically healthy dogs for occult disease. K A ... Hypercalcemia in these cases is caused by secretion of parathyroid hormone-related protein. Multicentric lymphoma presents as ...
C-reactive protein (CRP) is an acute phase protein. Therefore, it is a better marker for acute phase reaction than ESR. While ... mediates suppression of C-reactive protein: Explanation for muted C-reactive protein response in lupus flares?". Arthritis & ... Arik N, Bedir A, Günaydin M, Adam B, Halefi I (October 2000). "Do erythrocyte sedimentation rate and C-reactive protein levels ... ESR begins to rise at 24 to 48 hours after the onset of acute self-limited inflammation, decreases slowly as inflammation ...
This gene encodes a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is ... "Acute phase protein alpha 1-acid glycoprotein interacts with plasminogen activator inhibitor type 1 and stabilizes its ... sequence homology with other human acute phase protein genes". Nucleic Acids Res. 13 (11): 3941-52. doi:10.1093/nar/13.11.3941 ... classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however, it may be ...
As ferritin is also an acute-phase reactant, it is often elevated in the course of disease. A normal C-reactive protein can be ... Ferritin is a globular protein complex consisting of 24 protein subunits forming a hollow nanocage with multiple metal-protein ... where ferritin is elevated in its capacity as an inflammatory acute phase protein and not as a marker for iron overload.[ ... this implies that it is an acute phase protein. Mitochondrial ferritin has many roles pertaining to molecular function. It ...
April 2012). "Acute phase proteins in the diagnosis and prediction of cirrhosis associated bacterial infections". Liver ... It is also used as a validated survival predictor of cirrhosis, alcoholic hepatitis, acute liver failure, and acute hepatitis. ... Protein uptake is encouraged. The underlying cause may also need to be identified and treated. Causes include alcohol use, ... A low protein diet is recommended with gastrointestinal bleeding. Rifaximin is administered if mental state does not improve in ...
1993). "Ciliary neurotrophic factor induces acute-phase protein expression in hepatocytes". FEBS Lett. 314 (3): 280-4. doi: ... "Ciliary neurotrophic factor induces acute-phase protein expression in hepatocytes". FEBS Lett. 314 (3): 280-4. doi:10.1016/0014 ... Ciliary neurotrophic factor is a protein that in humans is encoded by the CNTF gene. The protein encoded by this gene is a ... Phase III clinical trials for the drug against obesity were conducted in 2003 by Axokine's maker, Regeneron Pharmaceuticals, ...
Schooltink H, Stoyan T, Roeb E, Heinrich PC, Rose-John S (Dec 1992). "Ciliary neurotrophic factor induces acute-phase protein ... Schooltink H, Stoyan T, Roeb E, Heinrich PC, Rose-John S (1992). "Ciliary neurotrophic factor induces acute-phase protein ... PTH-related protein receptor gene". Endocrinology. 140 (2): 925-32. doi:10.1210/endo.140.2.6573. PMID 9927325. IL6R+protein,+ ... The IL6 receptor is a protein complex consisting of an IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer ...
Ceruloplasmin is an acute phase protein synthesized in the liver. It is the carrier of the copper ion. Its level is increased ... In acute viral hepatitis, the GGT levels can peak at 2nd and 3rd week of illness, and remained elevated at 6 weeks of illness. ... Albumin is a protein made specifically by the liver, and can be measured cheaply and easily. It is the main constituent of ... In acute appendicitis, total bilirubin can rise from 20.52 μmol/L to 143 μmol/L. In pregnant women, the total bilirubin level ...
"Relationship of TSG-14 protein to the pentraxin family of major acute phase proteins". Journal of Immunology. 153 (8): 3700-7. ... PTX3 behaves as an acute phase response protein, as the blood levels of PTX3, low in normal conditions (about 25 ng/mL in the ... Pentraxin-related protein PTX3 also known as TNF-inducible gene 14 protein (TSG-14) is a protein that in humans is encoded by ... is a novel member of the pentaxin family of acute phase proteins". Journal of Immunology. 150 (5): 1804-12. PMID 7679696. Alles ...
1990). "Serum amyloid A, an acute phase protein, inhibits platelet activation". J. Lab. Clin. Med. 116 (2): 180-6. PMID 1697614 ... Ancsin JB, Kisilevsky R (1997). "Characterization of high affinity binding between laminin and the acute-phase protein, serum ... an acute-phase serum amyloid A protein gene (SAA2)". Genomics. 16 (2): 447-54. doi:10.1006/geno.1993.1209. PMID 7686132. " ... "Entrez Gene: SAA2 Serum amyloid A2". Betts JC, Edbrooke MR, Thakker RV, Woo P (1991). "The human acute-phase serum amyloid A ...
Since those are Acute-phase proteins, a positive Rivalta's test may be suggestive of inflammation. To perform this test, a ... An estimate of the concentration of protein in such fluids can narrow the differential diagnosis and assist the clinician in ... Using a pH 4.0 acetic acid solution, 8 types of proteins were identified in Rivalta reaction-positive turbid precipitates: C- ... Not only the high protein content, but high concentrations of fibrinogen and inflammatory mediators lead to a positive reaction ...
"Complex of soluble human IL-6-receptor/IL-6 up-regulates expression of acute-phase proteins". The Journal of Immunology. 149 (6 ... The Hyper-IL-6 protein has also been used to explore the physiologic role of Interleukin-6 trans-signaling in vivo. It turned ... Hyper-IL-6 is a fusion protein of the four-helical cytokine Interleukin-6 and the soluble Interleukin-6 receptor which are ... The complex of Interleukin-6 and the Interleukin-6 receptor associate with a second receptor protein called gp130, which ...
Acute-phase serum amyloid A proteins (A-SAAs) are secreted during the acute phase of inflammation. These proteins have several ... Protein pages needing a picture, Genes on human chromosome 11, Amyloidosis, Immune system, Acute-phase proteins). ... Serum amyloid A (SAA) is also an acute phase marker that responds rapidly. Similar to CRP, levels of acute-phase SAA increase ... an acute-phase serum amyloid A protein gene (SAA2)". Genomics. 16 (2): 447-54. doi:10.1006/geno.1993.1209. PMID 7686132. de ...
The presence or increased concentrations of acute phase proteins, particularly fibrinogen, results in enhanced erythrocyte ... Rouleaux formation takes place only in suspensions of RBC containing high-molecular, fibrilar proteins or polymers in the ... Erythrocyte aggregation is determined by both suspending phase (blood plasma) and cellular properties. Surface properties of ... suspending medium (often Dextran-2000 in-vitro). The most important protein causing rouleaux formation in plasma is fibrinogen ...
These globular regions of C1q can also bind to bacterial and viral surface proteins, apoptotic cells, and acute phase proteins ... The classical complement pathway can also be activated by apoptotic cells, necrotic cells, and acute phase proteins. The ... In addition, the C5 convertase initiates the terminal phase of the complement system, leading to the assembly of the membrane ... C2a diffuses into the plasma as a protein inflammatory mediator while C2b remains attached with C4b forming C4bC2b what is ...
In 1999, he became director of the University College London Centre for Amyloidosis and Acute Phase Proteins. Mark Pepys has ... He has pioneered work on the structure, function and clinical applications of the pentraxins, C-reactive protein (CRP) and ... the pentraxin proteins, and amyloidosis, and is a leading authority on these subjects in the UK and internationally. He ... and identified SAP as a normal tissue protein. He introduced radiolabelled SAP as a diagnostic in-vivo tracer for amyloid, ...
... an adaptor/docking protein, modulates STAT3 activation in acute-phase response through its YXXQ motif". J. Biol. Chem. 278 (13 ... Signal-transducing adaptor protein 1 is a protein that in humans is encoded by the STAP1 gene. The protein encoded by this gene ... The protein is directly phosphorylated by Tec in vitro where it participates in a positive feedback loop, increasing Tec ... A mouse ortholog, stem cell adaptor protein 1, shares 83% identity with its human counterpart. STAP1 has been shown to interact ...
"S-phase-specific interaction of the Fanconi anemia protein, FANCD2, with BRCA1 and RAD51". Blood. 100 (7): 2414-20. doi:10.1182 ... Acute myeloid leukemia[edit]. FA patients are at elevated risk for the development of AML defined as presence of 20% or more of ... Recent studies have shown that eight of these proteins, FANCA, -B, -C, -E, -F, -G, -L and -M, assemble to form a core protein ... Following assembly, the protein core complex activates FANCL protein which acts as an E3 ubiquitin-ligase and monoubiquitinates ...
Stadtman E (1992). "Protein oxidation and aging". Science. 257 (5074): 1220-4. doi:10.1126/science.1355616. PMID 1355616.. ... 2006). "NXY-059 for acute ischemic stroke". N Engl J Med. 354 (6): 588-600. doi:10.1056/NEJMoa052980. PMID 16467546.. ... and four retinyl esters in serum determined simultaneously by reversed-phase HPLC with multiwavelength detection" (PDF). Clin ... Sohal R (2002). "Role of oxidative stress and protein oxidation in the aging process". Free Radic Biol Med. 33 (1): 37-44. doi: ...
They added a protein called "human recombinant soluble angiotensin converting enzyme 2" (hrsACE2) and saw that it stopped the ... Pneumonia, viral sepsis, acute respiratory distress syndrome, kidney failure, cytokine release syndrome, respiratory failure, ... "The three phases of Covid-19 - and how we can make it manageable". The Spinoff. Retrieved 9 March 2020 ... They say mRNA vaccines take less time to develop and make than protein or whole-virus vaccines.[52][53] ...
Alpha-tocopherol transfer protein is coded by the TTPA gene on chromosome 8. The binding site for RRR-α-tocopherol is a ... Panin G, Strumia R, Ursini F (December 2004). "Topical alpha-tocopherol acetate in the bulk phase: eight years of experience in ... "Vitamin E Acetate as a Plausible Cause of Acute Vaping-related Illness". Cureus. 11 (12): e6350. doi:10.7759/cureus.6350. PMC ... Vitamin E affects gene expression[22] and is an enzyme activity regulator, such as for protein kinase C (PKC) - which plays a ...
severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), Severe acute respiratory syndrome ... It is a recombinant form of the protein Interferon alpha-2 that was originally sequenced and produced recombinantly in E. coli[ ... Weissmann, Charles (2001). "Recombinant interferon - the 20th anniversary". In Buckel, Peter (ed.). Recombinant Protein Drugs. ... but the period of usefulness is limited by the production of antibodies against this foreign protein.[9] ...
The first phase of collectivization resulted in modest improvements in output. Famine along the mid-Yangzi was averted in 1956 ... "In the first case, blows to the body caused internal injuries that, in combination with physical emaciation and acute hunger, ...
The acute phase occurs during the initial hours to days of implantation, and is identified by fluid and protein exudation along ... The inflammatory response occurs within two time periods: the acute phase, and the chronic phase. ... During the acute phase, the body attempts to clean and heal the wound by delivering excess blood, proteins, and monocytes are ... Cellulose and starch, proteins and peptides, and DNA and RNA are all examples of biopolymers, in which the monomeric units, ...
Phase relationships in the uranium-oxygen system are complex. The most important oxidation states of uranium are uranium(IV) ... Elevated levels of protein excretion, urinary catalase and diuresis Damage to proximal convoluted tubules, necrotic cells cast ... Acute cholinergic toxicity; Dose-dependent accumulation in cortex, midbrain, and vermis; Electrophysiological changes in ... Th(-I) and U(-I) have been detected in the gas phase as octacarbonyl anions; see Chaoxian, Chi; Sudip, Pan; Jiaye, Jin; Luyan, ...
"Protein Engineering Design and Selection. 24 (9): 633-34. doi:10.1093/protein/gzr012.. ... Kiernan, Matthew C.; Isbister, Geoffrey K.; Cindy; Lin, S.-Y.; Burke, David; Bostock, Hugh (2005). "Acute Tetrodotoxin-induced ... which also happens to be largely responsible for the sodium current that drives the depolarization phase of neuron action ... Konopacka, Agnieszka; Konopacki, Filip A.; Albrecht, Jan (2009). "Protein Kinase G Is Involved in Ammonia-induced Swelling of ...
A normal C-reactive protein can be used to exclude elevated ferritin caused by acute phase reactions.[citation needed] ... this implies that it is an acute phase protein.[27]. MitochondriaEdit. Mitochondrial ferritin has many roles pertaining to ... where ferritin is elevated in its capacity as an inflammatory acute phase protein and not as a marker for iron overload.[ ... Ferritin is a globular protein complex consisting of 24 protein subunits forming a hollow nanocage with multiple metal-protein ...
Hanash SM (September 2000). "Operomics: molecular analysis of tissues from DNA to RNA to protein". Clin. Chem. Lab. Med. 38 (9 ... A human Phase II clinical trial, with 41 patients, evaluated one synthetic lethal approach for tumors with or without MMR ... The first cancer genome was sequenced in 2008.[6] This study sequenced a typical acute myeloid leukaemia (AML) genome and its ... This treatment is also being evaluated for breast cancer and numerous other cancers in Phase III clinical trials in 2016.[47] ...
alfa-1 antitripsin (Inggris: Transcortin, corticosteroid-binding globulin, CBG) ingaran juga acute phase reactant[1] utawa ... "Total Serum Protein". WebMD.. (kaunduh 12/1/13) *↑ "Albumin bovine, Agarose, Affinity matrix" (pdf). Sigma-Aldrich. Dibukak ing ... Protein wewenang ,1%. mengatur èksprèsi genetik Ana 2 jinis protéin kang utama ing jero serum, yaiku albumin lan globulin. ... Protein darah. Kadar normal level. %. Kegunaan Serum albumin 3.5-5.0 g/dl. 60%. memelihara tekanan osmosis dan pengusung ...
"MDX 066, MDX 1388 Medarex, University of Massachusetts Medical School clinical data (phase II)(diarrhea)". Archived from the ... The protective effects of serum albumin may be related to the capability of this protein to bind C. difficile toxin A and toxin ... 4.8 billion each year in excess health care costs for acute care facilities alone."[99] ... "op-line data from randomized, double-blind, placebo controlled Phase 2 clinical trial indicate statistically significant ...
The green fluorescent protein (GFP) is often used in genetics as a marker. Many substances, such as proteins, have significant ... Difference frequency mixing (i.e., f1 + f2 − f3) as an advantage over sum frequency mixing because the phase matching can ... In humans, excessive exposure to UV radiation can result in acute and chronic harmful effects on the eye's dioptric system and ... Many enzymes and proteins involved in modern mitosis and meiosis are similar to repair enzymes, and are believed to be evolved ...
In the ventilation phase of a ventilation/perfusion scan, a gaseous radionuclide xenon or technetium DTPA in an aerosol form ( ... and for this purpose the iodide isotope does not need to be attached to another protein or molecule, because thyroid tissue ... The perfusion phase of the test involves the intravenous injection of radioactive technetium macro aggregated albumin (Tc99m- ... MAA). A gamma camera acquires the images for both phases of the study. ...
Within this protein-coding DNA (called the exome), an average cancer of the breast or colon can have about 60 to 70 protein ... The Roman medical encyclopedist Celsus (c. 30 BC-38 AD) described the four cardinal signs of acute inflammation as tumor, dolor ... point out that more than half of somatic mutations identified in tumors occurred in a pre-neoplastic phase (in a field defect ... Cancers are known to exhibit genome instability or a mutator phenotype.[56] The protein-coding DNA within the nucleus is about ...
"Acute Pancreatitis - Gastrointestinal Disorders". Merck Manuals Professional Edition. Merck.. [permanent dead link] ... Specific amylase proteins are designated by different Greek letters. All amylases are glycoside hydrolases and act on α-1,4- ... "Blocking saccharide absorption and weight loss: a clinical trial using Phase 2 brand proprietary fractionated white bean ... A higher than normal concentration may reflect any of several medical conditions, including acute inflammation of the pancreas ...
In the early phase of drug development, animal models were the only way of obtaining in vivo data that would predict the human ... The production of urea and steady-state protein proves the viability of this device for use in high-throughput toxicity studies ... It allows patients, especially out-patients, to self-serve the drugs with minimal possibility of experiencing acute drug ... Although PDMS is cheap, malleable, and transparent, proteins and small molecules can be absorbed by it and later leech at ...
By impact on protein sequence[edit]. The structure of a eukaryotic protein-coding gene. A mutation in the protein coding region ... Acute myeloblastic leukemia with maturation t(8 RUNX1T1;21 RUNX1). *Acute promyelocytic leukemia t(15 PML,17 RARA) ... The compensatory mutation can occur in the same protein or in another protein with which it interacts [112]. ... Thus, compensatory mutations can bring novelty to proteins by forging new pathways of protein evolution : it allows individuals ...
... are in phase 1 trials as of December 2021.[9][67] Subcutaneous injection of the anti-IgE antibody omalizumab is being studied ... Anaphylaxis to penicillin or cephalosporins occurs only after it binds to proteins inside the body with some agents binding ... "Acute symptoms of drug hypersensitivity (urticaria, angioedema, anaphylaxis, anaphylactic shock)" (PDF). The Medical Clinics ...
The drug is 95% bound to plasma protein. Its primary route of metabolism is by CYP3A4. It does not induce P450 enzymes. About ... acute psychosis, hallucinations, delusions, paranoia) and delirium (delirium, confusional state, disorientation, memory ...
The initial stage, or acute phase, is accompanied by mild symptoms such as lethargy, anorexia, fever, and lymphadenopathy ( ... The capsid protein derived from the polyprotein Gag is assembled into a viral core (the protein shell of a virus) and the ... The protein is prepared in a lyophilized (freeze-dried) 1 microgram dose. Reconstitution in sterile diluent produces a solution ... The bovine protein is homologous with other mammalian species and is a homogeneous 50 kDa glycoprotein with an isoelectric ...
Naziv leukemijski virus nosi sličan protein. Simbol BCR je izveden iz regiona klastera tačke prekida, gena koji kodira protein ... ciljanjem na JAK-STAT i BCR-ABL faze istovremeno. Inhibitori malih molekula, kao što su analozi arsenik-trioksida i ... Ikaros gene deletions in pediatric Philadelphia chromosome negative and Philadelphia chromosome positive B-cell precursor acute ... Ovaj gen je ABL1 na hromosomu 9 klasterske regije tačke prekida gena BCR sa hromosoma 22, kodirajući za hibridni protein: ...
... and their realization that this protein is the same as the Bence-Jones protein described in 1845 by Henry Bence Jones.[73] ... Animal toxicity studies: Acute toxicity testing, repeat-dose toxicity testing, long-term toxicity testing ... Multimodality Biomolecule Generation from the Solid Phase". Bioconjugate Chemistry. 27 (5): 1390-1399. doi:10.1021/acs. ... and database search methods that use existing protein sequence databases.[119][120] Many versions of shotgun protein sequencing ...
Acetylation of cellular proteins is a well-established phenomenon in the regulation of protein function at the post- ... Aspirin overdose can be acute or chronic. In acute poisoning, a single large dose is taken; in chronic poisoning, higher than ... "Resolution phase lipid mediators of inflammation: agonists of resolution". Current Opinion in Pharmacology. 13 (4): 632-40. ... Protein binding. 80-90%[3]. Metabolism. Liver (CYP2C19 and possibly CYP3A), some is also hydrolysed to salicylate in the gut ...
Phase 2b Controlled Trial of M72/AS01E Vaccine to Prevent Tuberculosis. New England Journal of Medicine. 2018-10-25, 379 (17): ... Jacob, Jesse T.; Mehta, Aneesh K.; Leonard, Michael K. Acute Forms of Tuberculosis in Adults. The American Journal of Medicine ... Niederweis M, Danilchanka O, Huff J, Hoffmann C, Engelhardt H. Mycobacterial outer membranes: in search of proteins. Trends in ...
The percentage of the drug that is protein bound is 98%.[15] ... "Acid-suppressive medication use in acute stroke and hospital- ... "Acute allergic interstitial nephritis after use of pantoprazole". CMAJ. 180 (5): 535-538. doi:10.1503/cmaj.080456. PMC 2645468 ...
"Plasma catecholamines in the acute phase of the response to myocardial infarction". Arch Emerg Med 3 (1): 20-7. பப்மெட்:3524599 ... 2006). "The novel role of C-reactive protein in cardiovascular disease: risk marker or pathogen". Int J Cardiol 106 (3): 291-7 ... Muller JE, Stone PH, Turi ZG, et al. (1985). "Circadian variation in the frequency of onset of acute myocardial infarction". N ... 29.0 29.1 Nyboe J, Jensen G, Appleyard M, Schnohr P. (1989). "Risk factors for acute myocardial infarction in Copenhagen. I: ...
... of people in the acute phase, but only 20-60% of those in the chronic phase.[6] Treatment of chronic disease is more effective ... cruzi proteins in donor blood.[31] ... During the acute phase of infection, it can be detected by ... In the acute phase of the disease, signs and symptoms are caused directly by the replication of T. cruzi and the immune system' ... T. cruzi shed acute-phase antigen (SAPA), which can be detected in blood using ELISA or Western blot,[22] has been used as an ...
Interests in acute phase proteins (APP) as biomarkers for the early progression of Alzheimers disease indicate that the ... Ng, P.M., Jin, Z., Tan, S.S., Ho, B. and Ding, J.L. (2004) C-Reactive Protein: A Predominant LPS-Binding Acute Phase Protein ... Hepatic Expression of Multiple Acute Phase Proteins and Down-Regulation of Nuclear Receptors after Acute Endotoxin Exposure. ... Interests in acute phase proteins (APP) as biomarkers for the early progression of Alzheimers disease indicate that the ...
... Pihl, Tina H.; Scheepers, Elrien; Sanz, Macarena; Goddard, ... MEASUREMENTS AND MAIN RESULTS : Blood and PF samples collected at admission were used for acute-phase protein concentration ... OBJECTIVE : To investigate the diagnostic potential of the concentrations of acute-phase proteins serum amyloid A (SAA), ... Blood and PF samples collected at admission were used for acute-phase protein concentration measurement. ...
The serum level of C-reactive protein (CRP) is associated with cognitive performance in acute phase psychosis. Johnsen, Erik; ... and cognitive performance in patients through the acute phase of psychosis. Methods A total of 124 patients were assessed at ... of the present study was to investigate possible associations between the level of the inflammation marker C-reactive protein ( ... The findings indicate that signs of inflammation may serve as a state-dependent marker of cognitive dysfunctions in acute ...
Acute-phase protein is raised in inflammatory conditions.. *When there is an increase in a protein called positive acute-phase ... protein. *In the case of a decrease in the acute phase protein, is called negative phase protein. ... The acute phase proteins (positive) are protein whose concentration increases in the plasma and after the disease episode is ... This is decreased in acute inflammation and raised in a chronic condition. *This acts as an acute-phase protein. ...
Acute phase proteins in tuberculous patients. The Indian Journal of Chest Diseases & Allied Sciences. 1990 Jan-Mar; 32(1): 15- ... The serum concentrations of some acute phase proteins were determined on admission, during treatment, at the end of treatment ... alpha 2-macroglobulin did not appear to function as an acute phase reactant in any of the 3 groups. Amalgamating the findings ... There was a significant decrease with treatment in the concentrations of C-reactive protein, ceruloplasmin, haptoglobin and ...
Acute phase proteins in visceral leishmaniasis / K.M.A. Wasunna. By: Wasunna, Kevin Monique AjilongContributor(s): UNDP/World ... Acute-phase proteins , Dissertations, Academic , Parasitic Diseases and their ControlNLM classification: WC 715 86WA ...
Acute rheumatic fever (ARF) is an autoimmune inflammatory process that develops as a sequela of streptococcal infection. ARF ... Acute-phase reactants, erythrocyte sedimentation rate, and C-reactive protein. Acute-phase reactants, the erythrocyte ... Acute rheumatic fever: a chink in the chain that links the heart to the throat?. Lancet Infect Dis. 2004 Apr. 4(4):240-5. [QxMD ... Resurgence of acute rheumatic fever in the intermountain area of the United States. N Engl J Med. 1987 Feb 19. 316(8):421-7. [ ...
QconCAT for equine acute phase proteins. QconCAT , Mass spectrometry , Proteomics. Tags: Beynon,Harman,Brownridge,QconCAT, ...
TextPublication details: 1991. Description: 367 leavesSubject(s): Acute-phase proteins , Dissertations, Academic , ... Acute phase proteins as predictors of disease activity in tropical infections : visceral leishmaniasis and tuberculosis / Kevin ... Details for: Acute phase proteins as predictors of disease activity in tropical infections : ...
The pathogenesis of CLA is a slow process, and produces a chronic rather than an acute disease state. Acute phase proteins (APP ... for AGP which occurred at a point when the infection was likely to have been transforming from an acute to a chronic phase. The ... Table 1 Area under curves for the time course of acute phase protein and anti-PLD (IgG) following C. pseudotuberculosis ... From: Acute phase protein response in an experimental model of ovine caseous lymphadenitis ...
Identification of sequences responsible for acute-phase induction of human C-reactive protein. / Arcone, R.; Gualandi, G.; ... Identification of sequences responsible for acute-phase induction of human C-reactive protein. Nucleic Acids Research. 1988;16( ... title = "Identification of sequences responsible for acute-phase induction of human C-reactive protein", ... Identification of sequences responsible for acute-phase induction of human C-reactive protein. ...
CINC-1 is an early hepatic acute phase protein induced by focal brain injury that causes leukocyte mobilization and liver ... CINC-1 is an early hepatic acute phase protein induced by focal brain injury that causes leukocyte mobilization and liver ...
It is one of a group of proteins, called acute phase reactants, that go up in response ... C-reactive protein (CRP) is produced by the liver. The level of CRP rises when there is inflammation throughout the body. ... It is one of a group of proteins, called acute phase reactants, that go up in response to inflammation. The levels of acute ... Haberman R, Fors Nieves CE, Cronstein BN, Saxena A. Acute phase reactants. In: Firestein GS, Budd RC, Gabriel SE, Koretzky GA, ...
ADAM Proteins / genetics * Acute-Phase Proteins / genetics * CD55 Antigens / genetics * Carrier Proteins / genetics ...
protein answers are found in the Tabers Medical Dictionary powered by Unbound Medicine. Available for iPhone, iPad, Android, ... Acute-phase proteins include C-reactive protein, several complement and coagulation factors, transport proteins, amyloid, and ... lipopolysaccharide-binding protein. One of many acute-phase proteins released into the serum in patients with a gram-negative ... acute phase protein. Any of the plasma proteins whose concentration increases or decreases by at least 25% during inflammation ...
Categories: Acute-Phase Proteins Image Types: Photo, Illustrations, Video, Color, Black&White, PublicDomain, ...
These proteins, primarily innate immune response proteins or proteins specific to liver, skin, or white blood cells, may serve ... In an independent cohort of patients with erythema migrans, six of these proteins, APOA4, C9, CRP, CST6, PGLYRP2, and S100A9, ... In a longitudinal cohort of 40 Lyme disease patients and 20 healthy controls, we identified 10 proteins with significantly ... Here, we performed selected reaction monitoring, a targeted mass spectrometry-based approach, to measure selected proteins that ...
C-reactive protein (CRP) is an acute phase protein synthesized in the liver. It is involved in the activation of complement, ... HS C-Reactive Protein (mg/L). English Text: High-Sensitivity C-Reactive Protein (hs-CRP) (mg/L). Target: Both males and females ... HS C-Reactive Protein Comment Code. English Text: High-Sensitivity C-Reactive Protein (hs-CRP) Comment Code. Target: Both males ... High-Sensitivity C-Reactive Protein (P_HSCRP). Data File: P_HSCRP.xpt. First Published: August 2021. Last Revised: NA. ...
In exchange, serum acute phase proteins (e.g. C-reactive protein, serum amyloid P) and accompanying liver gene expression were ... We evaluated serum proteins and blood cell gene expression of biomarkers related to potential cardiovascular effects. ... At 4hr, a marked systemic inflammatory response was evidenced by increased inflammatory serum proteins (e.g. IL-6, CXCL1) and ... At 24hr, inflammatory serum proteins and blood cell gene expression had returned to baseline and the systemic tissue response ...
Inflammatory markers like C-reactive protein (CRP) have been associated with post-traumatic stress disorder (PTSD) and ... and acute-phase proteins (e.g., CRP) have been observed in the blood of individuals with PTSD [4, 5]. Meta-analyses of cross- ... C-reactive protein as a predictor of posttraumatic stress induced by acute myocardial infarction. Gen Hosp Psychiatry. 2018;53: ... 2: Genetic correlation (x-axis: genetic correlation, rg; y-axis: -log10p-value) of serum C-reactive protein (CRP) with post- ...
The Use of Acute Phase Proteins as Biomarkers of Diseases in Cattle and Swine By Csilla Tóthová, Oskar Nagy and Gabriel Kováč ... Acute Phase Proteins as Markers of Diseases in Farm Animals By Csilla Tóthová, Oskar Nagy, Herbert Seidel and Gabriel Kováč ... Acute Phase Proteins in Dairy Cows and Sows During the Periparturient Period By Gabriel Kováč, Csilla Tóthová, Oskar Nagy ... Transthyretin has shorter half-life (2-3 days) than that of albumin and belongs to negative acute phase proteins. This may ...
Acute phase proteins in patients with abdominal aortic aneurysms. J Cardiovasc Surg 1987: 28(5), 528 530 ... Acute phase proteins in patients with abdominal aortic aneurysms. J Cardiovasc Surg 1987: 28(5), 528 530 ... Provision of acute beds in inner London. Are all Thames Regions the same? (Letter:Comment). BMJ 1993: 306(6893), 1692 ...
Acute phase proteins have been used in monitoring the progress of infection. In the present study serum ceruloplasmin and ... The main aim of this study was to evaluate the role of Plasma advanced oxidation protein product (AOPP), protein carbonyl as ... The iodine is reactive with all the three major biochemical constituents namely proteins, lipids and carbohydrates. Present ... Carrageenan induced paw edema and cotton pellet granuloma were the models for acute and subacute inflammation respectively.... ...
Acute Phase Proteins, Inflammation, Immunoassays. Our laboratory conducts research related to acute phase proteins and their ... Acute phase proteins are produced by the liver in response to proinflammatory cytokine stimulation, primarily IL-1, IL-6, and ... Our laboratory has focused on the acute phase protein serum amyloid A (SAA). Within 18 hours following stimulation, SAA is ... We have prepared a variety of recombinant proteins and peptides that reflect the novel colostrum-associated amino acid sequence ...
Ferritin L is the sole serum ferritin constituent and a positive hepatic acute-phase protein. Shock. 2013;39:520-6. DOIPubMed ...
Acute phase proteins. Browse this term in LC Authorities Interleukins. Browse this term in LC Authorities Monokines. Browse ...
CRP is an acute-phase protein belonging to the pentraxin family. CRP is synthesized in the hepatocytes, primarily under the ... The salivary determinations of eotaxin, IFN-γ, macrophage inflammatory proteins- (MIP-) 1β, IL-1β, IL-6, IL-8, and TNF-α were ... Y.-Z. Guo, L. Pan, C.-J. du, D.-Q. Ren, and X.-M. Xie, "Association between C-reactive protein and risk of cancer: a meta- ... Cytokines are small proteins that in the past were called lymphokines or monokines depending on the cells that produced them. ...
  • The aim of the present study was to investigate possible associations between the level of the inflammation marker C-reactive protein (CRP) and cognitive performance in patients through the acute phase of psychosis. (
  • Conclusions The findings indicate that signs of inflammation may serve as a state-dependent marker of cognitive dysfunctions in acute psychosis. (
  • This is decreased in acute inflammation and raised in a chronic condition. (
  • It is one of a group of proteins, called acute phase reactants, that go up in response to inflammation. (
  • These proteins are produced by white blood cells during inflammation. (
  • Any of the plasma proteins whose concentration increases or decreases by at least 25% during inflammation. (
  • They help mediate both positive and negative effects of acute and chronic inflammation, including chemotaxis, phagocytosis, protection against oxygen radicals, and tissue repair. (
  • This feedback amplifies the cycle of inflammation-mediated events and the release of acute phase reactants (see the image below). (
  • Moreover, compared with sham groups, the terms "acute-phase response", "inflammation", and "protein binding" were significantly enriched in CCI groups. (
  • Due to acute inflammation, it can cause the plasma concentrations of ORM levels to rapidly increase in response. (
  • A serum protein is considered an acute phase protein (APP, a protein produced by the liver and circulating in the bloodstream) if it increases or decreases more than 25% in response to inflammation. (
  • In contrast, mild and moderate APPs fibrinogen and C-reactive protein, respectively, are already present at low levels in the blood and gradually increase only one- to tenfold in response to inflammation. (
  • This is called acute inflammation. (
  • CRP is an acute-phase protein produced by the liver during conditions of inflammation, bacterial infection, or tissue trauma. (
  • 4 In this model, intestinal inflammation results from covalent binding of the haptenising agent to autologous host proteins with subsequent stimulation of a delayed-type hypersensitivity to TNBS modified self antigens. (
  • Known as a biomarker of acute inflammation, many large-scale prospective studies demonstrate the association between CRP and chronic inflammation, including: cardiovascular disease (CVD), cerebrovascular accident (CVA) (ischaemic stroke), Alzheimer's Disease, and age-related macular degeneration 2 . (
  • This transcription factor governs the expression of target genes that are involved in encoding cytokines, chemokines, growth factors, cell adhesion molecules, and acute-phase proteins that regulate inflammation, cell proliferation, and apoptosis. (
  • They also revealed that IL-6 not only enhances antibody production but also has a wide range of functions, including its role as a key stimulus for hepatic production of C-reactive protein* 3 (CRP) during the acute phase of inflammation and as a growth factor for multiple myeloma. (
  • 3 CRP is a protein found in the blood in response to inflammation or tissue destruction. (
  • However, serum retinol and retinol binding protein (RBP) concentrations, two biomarkers often used to estimate VAD, are temporarily altered during the acute phase response, potentially overestimating the prevalence of VAD in populations with high levels of inflammation. (
  • In 22 nationally-representative surveys, we examined (1) the association between C-reactive protein (CRP) or α1-acid glycoprotein (AGP) and retinol or RBP, and (2) how different adjustment approaches for correcting for inflammation compare with one another. (
  • Th2 cells are critical in maintaining both the state of chronic and relapsing eosinophil-predominant inflammation and the acute hypersensitivity responses characteristic of the atopic diseases. (
  • Acute-phase reactants, the erythrocyte sedimentation rate (ESR), and C-reactive protein levels (CRP) are usually elevated at the onset of ARF and serve as a minor manifestation in the Jones criteria. (
  • The levels of acute phase reactants increase in response to certain inflammatory proteins called cytokines. (
  • [ 1 ] Thus, circulating levels of cytokines, prothrombotic factors, or acute phase reactants may play a role in precipitating acute stroke in the setting of diseased vessels, even when not stenotic. (
  • Finally, many proinflammatory cytokines and acute-phase reactants originate in the adipocyte. (
  • OBJECTIVE : To investigate the diagnostic potential of the concentrations of acute-phase proteins serum amyloid A (SAA), haptoglobin (Hp), and fibrinogen in blood and peritoneal fluid (PF) for differentiating horses with inflammatory colic (entero-colitis and peritonitis) from those with surgical colic. (
  • CONCLUSIONS : Evaluation of SAA in serum improved the ability to differentiate horses with acute inflammatory colic requiring medical treatment from horses with colic requiring surgery, as it allowed an additional 4% of horses to be correctly classified into medical and surgical cases. (
  • Acute-phase protein is raised in inflammatory conditions. (
  • At 4hr, a marked systemic inflammatory response was evidenced by increased inflammatory serum proteins (e.g. (
  • At 24hr, inflammatory serum proteins and blood cell gene expression had returned to baseline and the systemic tissue response had diminished. (
  • Inflammatory markers like C-reactive protein (CRP) have been associated with post-traumatic stress disorder (PTSD) and traumatic experiences, but the underlying mechanisms are unclear. (
  • Our laboratory conducts research related to acute phase proteins and their role in the inflammatory process. (
  • The prepartum luteolysis was associated with immune inflammatory responses ( p = 2.87e-14), including acute-phase reaction ( p = 4.10e-06). (
  • Background The purpose of this randomised double-blind double-dummy placebo-controlled trial was to investigate whether etanercept, a tumour necrosis factor α (TNFα) antagonist, would provide more effective anti-inflammatory treatment for acute exacerbations of chronic obstructive pulmonary disease (COPD) than prednisone. (
  • Does treatment of acute exacerbations of COPD with the TNFα antagonist etanercept provide safer, more effective, anti-inflammatory treatment than prednisone? (
  • The elevated levels of inflammatory cytokines and chemokines (e.g., interleukin [IL] 6, IL-8, macrophage inflammatory protein 1α and 1β, monocyte chemoattractant protein 1, and macrophage colony-stimulating factor) and immunomodulatory cytokines (e.g. (
  • Guillain-Barré syndrome (GBS) can be described as a collection of clinical syndromes that manifests as an acute inflammatory polyradiculoneuropathy with resultant weakness and diminished reflexes. (
  • The typical patient with GBS, which in most cases will manifest as acute inflammatory demyelinating polyradiculoneuropathy (AIDP), presents 2-4 weeks following a relatively benign respiratory or gastrointestinal illness with complaints of finger dysesthesias and proximal muscle weakness of the lower extremities. (
  • DEL-1 is a protein known to regulate inflammatory cell recruitment and acts. (
  • It has been postulated that chronic airway symptoms result from persistent late-phase inflammatory responses in situations of perennial allergen exposure. (
  • The safest approach to modulate the immune or inflammatory pathways and cellular mechanisms is to either enhance or prevent these natural responses by administration of recombinant proteins of immune modulators produced also by the cells in the body. (
  • Interests in acute phase proteins (APP) as biomarkers for the early progression of Alzheimer's disease indicate that the peripheral A β metabolism is perturbed and the role of nutritional diets are important to reduce APPs to maintain peripheral A β clearance with relevance to hepatic cholesterol homeostasis and brain amyloidosis. (
  • These proteins, primarily innate immune response proteins or proteins specific to liver, skin, or white blood cells, may serve as candidate blood biomarkers requiring further validation to aid in the laboratory diagnosis of early Lyme disease. (
  • We evaluated serum proteins and blood cell gene expression of biomarkers related to potential cardiovascular effects. (
  • For example, serum biomarkers, such as high sensitivity C-reactive protein (hsCRP) and cytokine levels, predict progression of atherosclerosis and risk of stroke. (
  • Identifying novel biomarkers for risk stratification in t-cell acute lymphoblastic leukemia (ALL). (
  • The production of acute phase proteins (APPs) is induced and regulated by cytokines. (
  • These include α1 acid glycoprotein, serum amyloid A, the C-reactive protein homolog pentraxin-3, the lipocalin 24p3, and a host of cytokines ( 17 ). (
  • Methods We enrolled 81 patients with acute exacerbations of COPD and randomly assigned them to treatment with either 40 mg oral prednisone given daily for 10 days or to 50 mg etanercept given subcutaneously at randomisation and 1 week later. (
  • Impaired Overall Survival in Young Patients With Acute Myeloid Leukemia and Variants in Genes Predisposing for Myeloid Malignancies. (
  • Clinical Significance of RAS Gene Mutations in Patients with Acute Myeloid Leukemia]. (
  • 5'-nucleotidase, cytosolic II genotype, and clinical outcome in patients with acute myeloid leukemia with intermediate-risk cytogenetics. (
  • The Heart Failure Society of America 2010 comprehensive heart failure practice guideline recommends considering endomyocardial biopsy for patients with acute deterioration of heart function of unknown origin that is not responding to medical treatment. (
  • We have prepared a variety of recombinant proteins and peptides that reflect the novel colostrum-associated amino acid sequence, and have determined that one functional role of this unique isoform of SAA causes increased mucin-3 production by intestined cells. (
  • Immunoglobulin (Ig) M and IgG against Hantaan recombinant nucleocapsid protein antigen were detected in the case-patient's acute-phase and convalescent-phase serum samples, respectively, by ELISA ( 4 , 5 ). (
  • Feline IL-8 Recombinant Protein is purified interleukin-8 produced in yeast. (
  • Within the last two decades, VPM has successfully managed and consulted the development of promising biopharmaceutical candidates, ranging from small molecules, recombinant proteins up to gene-modified live vaccines and ATMPs. (
  • Frequency and clinical impact of WT1 mutations in the context of CEBPA-mutated acute myeloid leukemia. (
  • Clinical Outcomes and Prognostic Factors of Allogeneic Hematopoietic Stem Cell Transplantation in the Treatment of Refractory/Relapsed Acute Myeloid Leukemia]. (
  • Molecular profiling of patients with cytogenetically normal acute myeloid leukemia and hyperleukocytosis. (
  • Allogeneic hematopoietic cell transplantation can overcome the adverse prognosis indicated by secondary-type mutations in de novo acute myeloid leukemia. (
  • The Company also has clinical data for the use of Zantrene as a chemotherapeutic agent with reduced cardiotoxicity in acute myeloid leukemia (AML), breast and ovarian cancers and is investigating its use in these areas. (
  • So far, several novel, genetically improved antitumor antibodies for the treatment of acute myeloid leukemia (AML) and acute lymphocytic leukemia (ALL) have been developed. (
  • In a phase III, AGILE study, ivosidenib and azacitidine significantly improved event-free survival (EFS), response, and overall survival (OS) as compared with placebo and azacitidine in patients with newly diagnosed IDH1 -mutated acute myeloid leukaemia (AML) who were ineligible for induction chemotherapy. (
  • Acute and convalescent sera, if available, are helpful for proving recent streptococcal infection. (
  • Acute phase proteins have been used in monitoring the progress of infection. (
  • a colostrum-derived protein with potential as a prophylactic for enteropathogenic E. coli infection. (
  • METHODS: 158 pediatric (2-120-month-old) patients suspected to have acute bacterial infection, based on clinical judgment in which other causes of SIRS were ruled out were included in the study. (
  • Outcome measures such as length of hospital stay, mortality, incidence of infection and sepsis, acute phase protein levels, and muscle fractional synthetic rate were compared between and within groups during the acute stay (admission to discharge). (
  • Detection of acute/recent DENV infection by both molecular (RT-PCR) and serological methods (IgM) was available in 19/24 (79.2%) participating laboratories. (
  • During acute HIV infection, HIV is highly infectious because the virus is multiplying at a very rapid rate. (
  • The project included a Phase I clinical trial with AntiBKV, an antibody targeting BK polyomavirus (BKV) infection in renal transplant patients. (
  • The iCARE Dengue NS1 Rapid test kit detects acute infection with the dengue virus by measuring the non-structural protein 1 (NS1) of the virus. (
  • This makes it difficult to distinguish acute infection from chronic infection. (
  • The iCARE Dengue NS1 Rapid Testing Kit detects non-structural protein 1 of the dengue virus, a marker of recent exposure or acute infection. (
  • The iCARE Dengue NS1 Rapid test kit detects acute infection with dengue virus by detecting a nonstructural protein (NS1) from dengue virus. (
  • Developed as a serum test, NS1 is as sensitive as molecular tests in the early stages of acute dengue infection. (
  • In the convalescent phase, a second specimen should be tested for IgM antibodies. (
  • In clinical medicine the erythrocyte sedimentation rate or serum C-reactive protein level sometimes is used as a marker of increased amounts of acute-phase proteins. (
  • Increases in CRP concentration are non-specific and should be used in conjunction with traditional clinical laboratory evaluation of acute coronary syndromes. (
  • Thus, the usefulness of serum proteins in the diagnosis of health disorders and the possible clinical application of the results of the electrophoretic separation of serum proteins in ruminants will be reviewed in this chapter. (
  • Patients with myocarditis have a clinical history of acute decompensation of heart failure, but they have no other underlying cardiac dysfunction or have low cardiac risk. (
  • The diagnosis of acute myocarditis is usually presumptive, based on patient demographics and the clinical course. (
  • We present the clinical and laboratory characteristics of acute invasive H. influenzae diseases in children admitted over a 4-year period to a tertiary paediatric ward of the Al-Ain medical district hospital, before vaccination became available in the United Arab Emirates. (
  • In this phase I, single-blind, partially randomized, placebo-controlled clinical trial, 40 healthy adult volunteers were administered single and multiple ascending intravenous doses of AntiBKV ( NCT05358106). (
  • For its customers from the pharmaceutical industry, academic research and small biotechnology companies, VPM helped develop their products from preclinical phase through clinical phases I-III and approval, managing the processes more cost-effectively and with higher success probabilities. (
  • Natural Killer (NK) cell therapy pipeline report encloses its detailed analysis of various drugs in different stages of clinical development, including phase II, I, preclinical and Discovery. (
  • In February 2021, Nektar has entered into a clinical trial collaboration and supply agreement with Merck (known as MSD outside the United States and Canada) for a Phase II/III study of Bempegaldesleukin. (
  • The respective protocols for clinical phase I/II studies, based on these clinical data, have been submitted to the regulatory authorities and clinical studies are scheduled to start in 2016. (
  • This clinical benefit is supported by favourable health-related quality of life, incidences of transfusion independence, and the expected constellation of adverse events associated with treatment for acute leukaemia. (
  • Data from a phase Ib study that involved 23 patients with newly diagnosed IDH1 -mutated AML showed encouraging clinical activity with ivosidenib and azacitidine combination therapy. (
  • This research study is a Phase II clinical trial. (
  • Phase II clinical trials test the safety and effectiveness of an investigational drug to learn whether the drug or drug combination works in treating a specific disease. (
  • Prebreeding phase: Clinical and analytical laboratory findings. (
  • Prebreeding phase: Clinical health findings. (
  • alpha 2-macroglobulin did not appear to function as an acute phase reactant in any of the 3 groups. (
  • For a response of ACR20, there had to be ≥20% improvement in swollen joint count, ≥20% improvement in painful/tender joint count, and ≥20% improvement in at least 3 of the following: subject's assessment of pain, global assessment of disease activity, assessment of physical function, or acute phase reactant (C-reactive protein or erythrocyte sedimentation rate). (
  • The serum concentrations of some acute phase proteins were determined on admission, during treatment, at the end of treatment and at 12 months after stopping treatment in 20 patients with pulmonary tuberculosis. (
  • There was a significant decrease with treatment in the concentrations of C-reactive protein, ceruloplasmin, haptoglobin and alpha-1-acid glycoprotein in all 3 groups of patients. (
  • Here, we performed selected reaction monitoring, a targeted mass spectrometry-based approach, to measure selected proteins that (1) are known to be predominantly expressed in one organ (i.e., organ-specific blood proteins) and whose blood concentrations may change as a result of Lyme disease, or (2) are involved in acute immune responses. (
  • The determination of transthyretin concentrations may be used as a diagnostic tool for some disease conditions in humans, but is more often used as a nutritional marker to assess protein-calorie malnutrition and as prognostic indicator in critically ill patients. (
  • Many disease processes can cause changes in the concentrations of serum proteins. (
  • Ninety-five proteins were recognized as present at different concentrations in analyzed groups and were annotated to intracellular pathways. (
  • Phase 1 of this cross-sectional study aimed to compare hair lead concentrations and establish the determinants of hair lead levels in 149 occupationally exposed individuals versus 177 non-occupationally exposed controls in the Lebanese population. (
  • In acute and chronic diseases. (
  • Acute exacerbations of chronic obstructive pulmonary disease (AECOPD) are characterised clinically by symptoms of worsening dyspnoea, cough, sputum production and sputum purulence, as well as by worsening of airflow obstruction. (
  • In general, treatment of either acute or chronic myocarditis is aimed at reducing congestion and improving cardiac hemodynamics in heart failure, as well as providing supportive therapy, with the hope of prolonging survival. (
  • Nephrological therapies can treat both acute and chronic hypercalcemia, and it seems to be effective also in secondary hyperparathyroidism (HPT), but in other situations, such as posttransplantation HPT, a surgical approach is often needed. (
  • The same surveys report that 30% to 40% of children suffer from stunting (chronic malnutrition), 10% of children suffer from emaciation or wasting (acute malnutrition), 50% of children under 5 years of age and 60% of pregnant women have iron deficiency anaemia, 10% to 40% of adolescents have iodine deficiency (goitre) and 25% of children under 5 years have vitamin A deficiency. (
  • IgE antibodies (but not other isotypes) are capable of passive transfer of both acute and LPR sensitivity to allergen challenge. (
  • They carry enzymes, antibodies (immunoglobulins) and other proteins. (
  • In the upcoming DKTK funding period, antibody developmental work will focus on further optimized bi- and trispecific reagents and fusion proteins that target solid tumors and contain checkpoint modulating antibodies. (
  • Antibodies are proteins normally made by the immune system that bind to substances that don't belong in the body to prevent harm. (
  • No single specific laboratory test can confirm the diagnosis of acute rheumatic fever (ARF). (
  • Adaptation of acute lymphoblastic leukemia (ALL) to the post-transplant allogeneic environment. (
  • Acute liver diseases. (
  • C-reactive protein (CRP) is produced by the liver. (
  • C-reactive protein (CRP) is an acute phase protein synthesized in the liver. (
  • C-reactive protein, serum amyloid P) and accompanying liver gene expression were increased. (
  • Acute phase proteins are produced by the liver in response to proinflammatory cytokine stimulation, primarily IL-1, IL-6, and TNFą. (
  • The serum albumin level was not affected by patient age and gender, type of myeloma, and the occurrence of Bence Jones protein, lytic bone lesions, or hypercalcemia, and it was not related to changes in body weight or in liver and renal function. (
  • Interestingly, fetuin-A, a liver-secreted acute-phase glycoprotein, was involved in these biological processes. (
  • It is an acute-phase protein produced in the liver as well as in extrahepatic sites. (
  • Three days after admission, acute renal failure with relative oliguria (0.85 L/24h) developed, as well as uremia (26.4 mg/dL), creatinemia (0.98 mg/dL), and abnormal liver function (aspartate aminotransferase 49 U/L and alanine transferase 60 U/L). The following day the patient had dyspnea and became agitated. (
  • INTERVENTION : Blood and PF samples collected at admission were used for acute-phase protein concentration measurement. (
  • The acute phase proteins (positive) are protein whose concentration increases in the plasma and after the disease episode is over then it decreases and may become normal. (
  • Impact of Dietary Protein Concentration and Quality on Immune Function of Cats. (
  • Amalgamating the findings in all 3 groups of tuberculous patients, the proportions of patients with abnormal values on admission and at the end of treatment were 62% and 14% for C-reactive protein, 78% and 50% for ceruloplasmin, 86% and 26% for haptoglobin and 92% and 6% for alpha 1-acid glycoprotein, respectively. (
  • This leads to a dysregulation of triglyceride and free fatty acid levels, as well as a dysregulation of specific adipocyte-derived secretory proteins, a group of proteins that we refer to as adipokines. (
  • A more sensitive CRP test, called a high-sensitivity C-reactive protein (hs-CRP) assay, is available to determine a person's risk for heart disease. (
  • He joined UCL in October 2011 with MRC Strategic Award, where he established a new group in the Centre for Amyloidosis and Acute Phase Proteins. (
  • He identified a previously unrecognized step in the development of transthyretin amyloidosis by demonstrating that stress-mediated proteolysis is a pivotal step in the protein fibrillogenesis pathway. (
  • Dietary proteins provide the amino acids necessary for the growth and repair of animal tissue. (
  • About 20 different amino acids make up human proteins, which may contain other minerals such as iron or copper. (
  • A protein consists of from 50 to thousands of amino acids arranged in a specific sequence. (
  • An incomplete protein lacks one or more of the essential amino acids. (
  • Salt-loaded, spontaneously hypertensive stroke-prone rats show progressive increases in blood pressure and proteinuria and accumulate acute-phase proteins in body fluids, modeling events during renal damage. (
  • Under physiological conditions, the production of serum proteins is closely regulated, but alterations in the serum protein pattern may occur in a wide range of diseases and health disorders. (
  • The postmenopausal phase of life is frequently associated in women with subjective symptoms (e.g. vasomotor) and real diseases (atherosclerosis with coronary ischemia, osteoporosis, Alzheimer-type neurodegeneration, urogenital dystrophy), which together determine the post-menopausal syndrome. (
  • It is thus obvious that additional proteins produced by the human cells in these diseases displaying a potential benefit for CNS diseases need to be identified. (
  • Two important types of allergic diseases at the workplace a symptomatic effector phase. (
  • Transthyretin has shorter half-life (2-3 days) than that of albumin and belongs to negative acute phase proteins. (
  • Patients with myocarditis usually present with signs and symptoms of acute decompensation of heart failure (eg, tachycardia, gallop, mitral regurgitation, edema) and, in those with concomitant pericarditis, with pericardial friction rub. (
  • The authors commented that this phase III study showed that combination therapy with ivosidenib and azacitidine was associated with adverse events similar to those attributed to treatment for acute leukaemia and was effective in extending EFS, increasing the likelihood of complete remission, and prolonging OS among patients with IDH1 -mutated AML who were older or otherwise ineligible for induction chemotherapy. (
  • Adapter proteins participate in the immune response by acting as a bridge for enzymes in the signaling pathway needed to activate lymphocytes and initiate a response to an antigen. (
  • Module A contained serum samples spiked with cultured dengue virus (DENV) or chikungunya virus (CHIKV) for the detection of nucleic acid and DENV non-structural protein 1 (NS1) antigen. (
  • Its nonstructural protein 1 (NS1) antigen provides a quick, accurate test result. (
  • Based on the results from the Phase 3 KEYNOTE-775/Study 309 trial, KEYTRUDA plus LENVIMA has been approved in the U.S. for patients with advanced endometrial carcinoma that is not microsatellite instability-high or dMMR, who have disease progression following prior systemic therapy in any setting and are not candidates for curative surgery or radiation. (
  • Blood will be collected for measurement of other markers of neurodegeneration and oxidative stress, including neurofilament light chain, isoprostanes, and acute phase proteins. (
  • Plasma levels of bacterial lipopolysaccharide (LPS) and fungal (1→3)-β-D-Glucan (BDG) translocation markers, along with markers of intestinal damage fatty acid binding protein (I-FABP) and regenerating islet-derived protein-3α (REG3α) were assessed by ELISA or the fungitell assay. (
  • Some might be testing for the amounts of certain proteins, while others may analyze the number of a specific type of cell. (
  • Some cells may also release certain proteins that activate other parts of the immune system. (
  • Furthermore, at both 4 and 24hr increased serum levels of the prothrombotic protein plasminogen activator inhibitor-1 were found. (
  • While storage and release of lipids are major functions of adipocytes, the adipocyte also uses specific lipid molecules for intracellular signaling and uses a host of protein factors to communicate with essentially every organ system in the body. (
  • ANIMALS : Horses referred for severe acute abdominal pain to Hospital 1 (n = 148) or Hospital 2 (n = 78). (
  • Heat stroke is a a recorded temperature high of 74°C, with an actual air result of the failure of the body to regulate its temperature temperature of 46°C and a high dew point temperature accordingly along with a severe acute-phase response of 32°C ( 3 ). (
  • Since the coronavirus disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was declared a pandemic by the World Health Organization on 11 March 2020, vaccine development and administration became a global priority. (
  • Compared to adults, children with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have predominantly mild or asymptomatic infections, but the underlying immunological differences remain unclear. (
  • Although hundreds of proteins exist in blood serum, little is known about the precise composition and entire set of serum proteins in different ruminant species. (
  • handle/10665/39485, consulté en janvier 2021). (
  • In protein malnutrition. (
  • 2. In the 1980s, many countries in the African Region made steady progress in reducing levels of protein energy malnutrition. (
  • 4. The major nutritional problems besetting the African Region are protein energy malnutrition and micronutrient deficiencies4. (
  • A protein that stimulates the expression of a gene. (
  • We hypothesized that blood cell gene expression and serum protein analysis will provide insight into the relationship between CNT-induced lung and cardiovascular effects. (
  • HMGB1 is a chromatin-binding protein that among several undertakings regulates gene transcription, but operates as a critical DAMP after being released. (
  • In addition to triggering immediate-hypersensitivity reactions and late-phase responses, there is accumulating evidence that preformed IgE can augment humoral and cellular immune responses to allergens. (
  • In response to the presence of certain activating substances, including oxidized LDL, monocyte chemoattractant protein 1 (MCP-1), interleukin (IL)-8, and platelet-derived growth factor (PDGF), leukocytes migrate into the wall of the artery (see the image below). (
  • 1973. Dose-response and ultrastructural alterations in dioxane carcinogenesis: Influence of methylcholanthrene on acute toxicity. (
  • The patients' levels of protein C, antithrombin III and in vitro platelet aggregation in response to collagen were compared with those of 20 controls. (
  • Because there is not a concrete known function for orosomucoid, it is of the opinion that orosomucoid (ORM) regulates the immune system in the acute-phase reaction. (
  • IgE-induced mast cell degranulation in vivo is often followed by a late-phase reaction (LPR), a second wave of hypersensitivity responses occurring many hours after the acute reaction and dependent upon eosinophils. (
  • Acute-phase proteins include C-reactive protein, several complement and coagulation factors, transport proteins, amyloid, and antiprotease enzymes. (
  • Bilirubin is a metabolite of haem, derived from haem-containing proteins, including myoglobin, haemoglobin and various P450 enzymes, that serves to coordinate iron in various proteins. (
  • Most of the APPs used in veterinary medicine are positive APPs, including SAA, C-reactive protein, and fibrinogen. (
  • 1990a, 1990b, 1995) reportaron un aumento de la proliferación celular en experimentos realizados utilizando células de un glioma cerebral, linfocitos humanos y células ováricas de Hámster. (
  • Los experimentos in Vivo de Lai y Singh (1995, 1996) ameritan especial atención, teniendo en cuenta el interés que despertaron. (
  • In a longitudinal cohort of 40 Lyme disease patients and 20 healthy controls, we identified 10 proteins with significantly altered serum levels in patients at the time of diagnosis, and we also developed a 10-protein panel identified through multivariate analysis. (
  • Evaluation of a rapid IgM detection test for diagnosis of acute leptospirosis in dogs. (
  • Elevated or rising protein levels on serial lumbar punctures and 10 or fewer mononuclear cells/mm 3 strongly support the diagnosis. (
  • Approved in July 2011 for reducing CV death & MI in patients of acute coronary syndrome. (
  • The serum protein profile is mostly evaluated by serum protein electrophoresis, which allows the identification of protein fractions, each being composed of several individual proteins with similar electrophoretic mobility. (
  • At UCL he has continued his research into the molecular mechanisms of amyloidogenesis as exemplified in two recent studies, which both arose out of his work following the identification of new pathogenic variants of amyloidogenic proteins. (
  • Identification of proteins interacting with ALK in neuroblastoma. (
  • The iodine is reactive with all the three major biochemical constituents namely proteins, lipids and carbohydrates. (
  • Erythrocyte sedimentation rate and C-reactive protein were not elevated. (
  • Evidence in humans and animal models suggests that IgE-mediated mast cell activation gives rise to both the acute and late-phase responses. (
  • Acute responses are accompanied by evidence of mast cell activation and mediator release. (
  • Durable and deep responses, with frequent IDH1 mutation clearance, occurred in patients who received ivosidenib and azacitidine, a finding that highlights the benefit of targeting the mutated IDH1 protein. (
  • The acute phase protein serum amyloid A (SAA) is a powerful tool for equine veterinarians. (
  • In an independent cohort of patients with erythema migrans, six of these proteins, APOA4, C9, CRP, CST6, PGLYRP2, and S100A9, were confirmed to show significantly altered serum levels in patients at time of presentation. (
  • Our quantitative proteomics identified 2499 proteins, 157 were significantly differentially expressed between brain tissues at 6 hours after CCI (CCI6h) and sham groups, and 109 were significantly differentially expressed between CCI24h and sham brain tissues. (
  • Representative standard curve using the Canine C-Reactive Protein [CRP] ELISA Kiit, 1x Standard. (
  • The Canine C-Reactive Protein (CRP) ELISA Kit is designed for the detection and quantitation of canine CRP in canine serum. (
  • This ELISA for canine CRP is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). (
  • This is an open label single-arm phase II study to evaluate the combination therapy of the antibody drug conjugate, sacituzumab govitecan, and the anti-PD-L1 antibody, atezolizumab, in patients with triple negative breast cancer . (
  • Plasma from both parents and one child have IgG antibody against the S1 protein and virus-neutralizing activity detected. (
  • Despite this usefulness, the evaluation of serum protein pattern is still relatively a less frequently used laboratory diagnostic technique in ruminant medicine. (
  • Our laboratory has focused on the acute phase protein serum amyloid A (SAA). (
  • His seminal demonstration that the amyloidogenic variants of lysozyme, discovered in the laboratory of Professor Sir Mark B. Pepys at the Royal Postgraduate Medical School, Hammersmith Hospital in 1993, were less stable than the wild type molecule provided the basis for a now generally accepted theory explaining the mechanism of amyloid conversion of globular proteins. (
  • In the case of a decrease in the acute phase protein, is called negative phase protein. (
  • Therefore, here, next-generation sequencing (RNA-Seq) was performed on CL from the late luteal phase and compared with normally luteolyzing CL collected at the prepartum P4 decrease. (
  • Whether sustained normocortisolism induced by medical therapy induces re-expression of functional sst 2 protein in corticotroph adenomas and whether this increases the ACTH-lowering potency of octreotide remains to be established. (
  • The proteins involved in nonsense-mediated decay and L13a-mediated translational silencing of ceruloplasmin expression were recognized as downregulated. (
  • These include work on alpha-synuclein (Professor Schapira), apolipoprotein C-III (Professor Talmud), structural insights into amyloidogenic proteins (Professor Christodoulou) and the generation of bio-compatible devices to study fibrillogenesis of human globular proteins (Professor Pinzani). (
  • Other external collaborations include the use of native mass spectrometry for protein structure analysis (Professor Robinson, Oxford) and work on new amyloidogenic variants of β2-microglobulin and apolipoprotein C-III (Professor Valleix, Paris). (
  • Measurement of Organ-Specific and Acute-Phase Blood Protein Levels in " by Yong Zhou, Shizhen Qin et al. (
  • The objective of this study was to determine whether the increased AKT or MAPK kinase-1/2 (MEK1/2) activity observed in endometriotic stromal cells (OSIS) from ovarian endometriomas influences levels of PR protein. (
  • Inhibiting AKT with MK-2206 or MEK1/2 with U0126 for 24 hours in the absence of R5020 increased total and nuclear PRA and PRB protein levels in OSIS but not in eutopic endometrial stromal cells from disease-free patients from disease-free patients. (
  • We evaluated receptor mRNA and protein expression levels and effects of octreotide, pasireotide, and cabergoline on ACTH secretion by cultured human corticotroph adenoma cells. (
  • During each short interglacial phase sea levels have been within ~10 m of today's level (0 m). (