AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation Science
ActomyosinMyosinsMyosin Type IIActinsMyosin SubfragmentsTropomyosinCytokinesisAdenosine TriphosphatasesCa(2+) Mg(2+)-ATPaseTroponinRabbitsHeterocyclic Compounds with 4 or More RingsActin CytoskeletonNonmuscle Myosin Type IIAMyofibrilsCytoskeletonMusclesCalmodulin-Binding ProteinsMuscle ContractionGizzardMyosin Light ChainsCell ShapeAdenosine TriphosphateMuscle ProteinsEthenoadenosine TriphosphateAdenosine DiphosphateMolecular Motor ProteinsChemical PrecipitationChickensMyosin Heavy ChainsStress FibersCalciumCytoplasmic Streamingrho-Associated KinasesKineticsSchizosaccharomycesSchizosaccharomyces pombe ProteinsPhalloidineTroponin INonmuscle Myosin Type IIBMicrofilament ProteinsCell PolarityTroponin CMuscle, SmoothMyosin-Light-Chain KinaseContractile ProteinsTime-Lapse ImagingGastrulationProtein BindingMuscle, SkeletalPsoas MusclesModels, BiologicalDiacetylMyosin Type VPhysarumIsometric Contraction
Actomyosin
A protein complex of actin and MYOSINS occurring in muscle. It is the essential contractile substance of muscle.
Myosins
A diverse superfamily of proteins that function as translocating proteins. They share the common characteristics of being able to bind ACTINS and hydrolyze MgATP. Myosins generally consist of heavy chains which are involved in locomotion, and light chains which are involved in regulation. Within the structure of myosin heavy chain are three domains: the head, the neck and the tail. The head region of the heavy chain contains the actin binding domain and MgATPase domain which provides energy for locomotion. The neck region is involved in binding the light-chains. The tail region provides the anchoring point that maintains the position of the heavy chain. The superfamily of myosins is organized into structural classes based upon the type and arrangement of the subunits they contain.
Myosin Type II
The subfamily of myosin proteins that are commonly found in muscle fibers. Myosin II is also involved a diverse array of cellular functions including cell division, transport within the GOLGI APPARATUS, and maintaining MICROVILLI structure.
Actins
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
Myosin Subfragments
Parts of the myosin molecule resulting from cleavage by proteolytic enzymes (PAPAIN; TRYPSIN; or CHYMOTRYPSIN) at well-localized regions. Study of these isolated fragments helps to delineate the functional roles of different parts of myosin. Two of the most common subfragments are myosin S-1 and myosin S-2. S-1 contains the heads of the heavy chains plus the light chains and S-2 contains part of the double-stranded, alpha-helical, heavy chain tail (myosin rod).
Tropomyosin
A protein found in the thin filaments of muscle fibers. It inhibits contraction of the muscle unless its position is modified by TROPONIN.
Cytokinesis
The process by which the CYTOPLASM of a cell is divided.
Adenosine Triphosphatases
A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.
Ca(2+) Mg(2+)-ATPase
Troponin
One of the minor protein components of skeletal muscle. Its function is to serve as the calcium-binding component in the troponin-tropomyosin B-actin-myosin complex by conferring calcium sensitivity to the cross-linked actin and myosin filaments.
Rabbits
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Heterocyclic Compounds with 4 or More Rings
A class of organic compounds containing four or more ring structures, one of which is made up of more than one kind of atom, usually carbon plus another atom. The heterocycle may be either aromatic or nonaromatic.
Actin Cytoskeleton
Fibers composed of MICROFILAMENT PROTEINS, which are predominately ACTIN. They are the smallest of the cytoskeletal filaments.
Nonmuscle Myosin Type IIA
A nonmuscle isoform of myosin type II found predominantly in platelets, lymphocytes, neutrophils and brush border enterocytes.
Myofibrils
The long cylindrical contractile organelles of STRIATED MUSCLE cells composed of ACTIN FILAMENTS; MYOSIN filaments; and other proteins organized in arrays of repeating units called SARCOMERES .
Cytoskeleton
The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.
Muscles
Contractile tissue that produces movement in animals.
Calmodulin-Binding Proteins
Proteins which bind calmodulin. They are found in many tissues and have a variety of functions including F-actin cross-linking properties, inhibition of cyclic nucleotide phosphodiesterase and calcium and magnesium ATPases.
Muscle Contraction
A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments.
Gizzard
Myosin Light Chains
The smaller subunits of MYOSINS that bind near the head groups of MYOSIN HEAVY CHAINS. The myosin light chains have a molecular weight of about 20 KDa and there are usually one essential and one regulatory pair of light chains associated with each heavy chain. Many myosin light chains that bind calcium are considered "calmodulin-like" proteins.
Cell Shape
The quality of surface form or outline of CELLS.
Adenosine Triphosphate
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
Muscle Proteins
The protein constituents of muscle, the major ones being ACTINS and MYOSINS. More than a dozen accessory proteins exist including TROPONIN; TROPOMYOSIN; and DYSTROPHIN.
Ethenoadenosine Triphosphate
1,N-6-Ethenoadenosine triphosphate. A fluorescent analog of adenosine triphosphate.
Adenosine Diphosphate
Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.
Molecular Motor Proteins
Proteins that are involved in or cause CELL MOVEMENT such as the rotary structures (flagellar motor) or the structures whose movement is directed along cytoskeletal filaments (MYOSIN; KINESIN; and DYNEIN motor families).
Chemical Precipitation
The formation of a solid in a solution as a result of a chemical reaction or the aggregation of soluble substances into complexes large enough to fall out of solution.
Chickens
Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.
Myosin Heavy Chains
The larger subunits of MYOSINS. The heavy chains have a molecular weight of about 230 kDa and each heavy chain is usually associated with a dissimilar pair of MYOSIN LIGHT CHAINS. The heavy chains possess actin-binding and ATPase activity.
Stress Fibers
Bundles of actin filaments (ACTIN CYTOSKELETON) and myosin-II that span across the cell attaching to the cell membrane at FOCAL ADHESIONS and to the network of INTERMEDIATE FILAMENTS that surrounds the nucleus.
Calcium
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Cytoplasmic Streaming
The movement of CYTOPLASM within a CELL. It serves as an internal transport system for moving essential substances throughout the cell, and in single-celled organisms, such as the AMOEBA, it is responsible for the movement (CELL MOVEMENT) of the entire cell.
rho-Associated Kinases
A group of intracellular-signaling serine threonine kinases that bind to RHO GTP-BINDING PROTEINS. They were originally found to mediate the effects of rhoA GTP-BINDING PROTEIN on the formation of STRESS FIBERS and FOCAL ADHESIONS. Rho-associated kinases have specificity for a variety of substrates including MYOSIN-LIGHT-CHAIN PHOSPHATASE and LIM KINASES.
Kinetics
The rate dynamics in chemical or physical systems.
Schizosaccharomyces
A genus of ascomycetous fungi of the family Schizosaccharomycetaceae, order Schizosaccharomycetales.
Schizosaccharomyces pombe Proteins
Proteins obtained from the species Schizosaccharomyces pombe. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
Phalloidine
Very toxic polypeptide isolated mainly from AMANITA phalloides (Agaricaceae) or death cup; causes fatal liver, kidney and CNS damage in mushroom poisoning; used in the study of liver damage.
Troponin I
One of the three polypeptide chains that make up the TROPONIN complex. It inhibits F-actin-myosin interactions.
Nonmuscle Myosin Type IIB
A nonmuscle isoform of myosin type II found predominantly in neuronal tissue.
Microfilament Proteins
Monomeric subunits of primarily globular ACTIN and found in the cytoplasmic matrix of almost all cells. They are often associated with microtubules and may play a role in cytoskeletal function and/or mediate movement of the cell or the organelles within the cell.
Cell Polarity
Orientation of intracellular structures especially with respect to the apical and basolateral domains of the plasma membrane. Polarized cells must direct proteins from the Golgi apparatus to the appropriate domain since tight junctions prevent proteins from diffusing between the two domains.
Troponin C
One of the three polypeptide chains that make up the TROPONIN complex of skeletal muscle. It is a calcium-binding protein.
Muscle, Smooth
Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed)
Myosin-Light-Chain Kinase
An enzyme that phosphorylates myosin light chains in the presence of ATP to yield myosin-light chain phosphate and ADP, and requires calcium and CALMODULIN. The 20-kDa light chain is phosphorylated more rapidly than any other acceptor, but light chains from other myosins and myosin itself can act as acceptors. The enzyme plays a central role in the regulation of smooth muscle contraction.
Contractile Proteins
Proteins which participate in contractile processes. They include MUSCLE PROTEINS as well as those found in other cells and tissues. In the latter, these proteins participate in localized contractile events in the cytoplasm, in motile activity, and in cell aggregation phenomena.
Time-Lapse Imaging
Recording serial images of a process at regular intervals spaced out over a longer period of time than the time in which the recordings will be played back.
Gastrulation
A process of complicated morphogenetic cell movements that reorganizes a bilayer embryo into one with three GERM LAYERS and specific orientation (dorsal/ventral; anterior/posterior). Gastrulation describes the germ layer development of a non-mammalian BLASTULA or that of a mammalian BLASTOCYST.
Protein Binding
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Muscle, Skeletal
A subtype of striated muscle, attached by TENDONS to the SKELETON. Skeletal muscles are innervated and their movement can be consciously controlled. They are also called voluntary muscles.
Psoas Muscles
A powerful flexor of the thigh at the hip joint (psoas major) and a weak flexor of the trunk and lumbar spinal column (psoas minor). Psoas is derived from the Greek "psoa", the plural meaning "muscles of the loin". It is a common site of infection manifesting as abscess (PSOAS ABSCESS). The psoas muscles and their fibers are also used frequently in experiments in muscle physiology.
Models, Biological
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Diacetyl
Carrier of aroma of butter, vinegar, coffee, and other foods.
Myosin Type V
A subclass of myosin involved in organelle transport and membrane targeting. It is abundantly found in nervous tissue and neurosecretory cells. The heavy chains of myosin V contain unusually long neck domains that are believed to aid in translocating molecules over large distances.
Physarum
A genus of protozoa, formerly also considered a fungus. Characteristics include the presence of violet to brown spores.
Isometric Contraction
Muscular contractions characterized by increase in tension without change in length.

Evidence for F-actin-dependent and -independent mechanisms involved in assembly and stability of the medial actomyosin ring in fission yeast. (1/982)

Cell division in a number of eukaryotes, including the fission yeast Schizosaccharomyces pombe, is achieved through a medially placed actomyosin-based contractile ring. Although several components of the actomyosin ring have been identified, the mechanisms regulating ring assembly are still not understood. Here, we show by biochemical and mutational studies that the S.pombe actomyosin ring component Cdc4p is a light chain associated with Myo2p, a myosin II heavy chain. Localization of Myo2p to the medial ring depended on Cdc4p function, whereas localization of Cdc4p at the division site was independent of Myo2p. Interestingly, the actin-binding and motor domains of Myo2p are not required for its accumulation at the division site although the motor activity of Myo2p is essential for assembly of a normal actomyosin ring. The initial assembly of Myo2p and Cdc4p at the division site requires a functional F-actin cytoskeleton. Once established, however, F-actin is not required for the maintenance of Cdc4p and Myo2p medial rings, suggesting that the attachment of Cdc4p and Myo2p to the division site involves proteins other than actin itself.  (+info)

Calculation of a Gap restoration in the membrane skeleton of the red blood cell: possible role for myosin II in local repair. (2/982)

Human red blood cells contain all of the elements involved in the formation of nonmuscle actomyosin II complexes (V. M. Fowler. 1986. J. Cell. Biochem. 31:1-9; 1996. Curr. Opin. Cell Biol. 8:86-96). No clear function has yet been attributed to these complexes. Using a mathematical model for the structure of the red blood cell spectrin skeleton (M. J. Saxton. 1992. J. Theor. Biol. 155:517-536), we have explored a possible role for myosin II bipolar minifilaments in the restoration of the membrane skeleton, which may be locally damaged by major mechanical or chemical stress. We propose that the establishment of stable links between distant antiparallel actin protofilaments after a local myosin II activation may initiate the repair of the disrupted area. We show that it is possible to define conditions in which the calculated number of myosin II minifilaments bound to actin protofilaments is consistent with the estimated number of myosin II minifilaments present in the red blood cells. A clear restoration effect can be observed when more than 50% of the spectrin polymers of a defined area are disrupted. It corresponds to a significant increase in the spectrin density in the protein free region of the membrane. This may be involved in a more complex repair process of the red blood cell membrane, which includes the vesiculation of the bilayer and the compaction of the disassembled spectrin network.  (+info)

Ca2+ and cross-bridge-induced changes in troponin C in skinned skeletal muscle fibers: effects of force inhibition. (3/982)

Changes in skeletal troponin C (sTnC) structure during thin filament activation by Ca2+ and strongly bound cross-bridge states were monitored by measuring the linear dichroism of the 5' isomer of iodoacetamidotetramethylrhodamine (5'IATR), attached to Cys98 (sTnC-5'ATR), in sTnC-5'ATR reconstituted single skinned fibers from rabbit psoas muscle. To isolate the effects of Ca2+ and cross-bridge binding on sTnC structure, maximum Ca2+-activated force was inhibited with 0.5 mM AlF4- or with 30 mM 2,3 butanedione-monoxime (BDM) during measurements of the Ca2+ dependence of force and dichroism. Dichroism was 0.08 +/- 0.01 (+/- SEM, n = 9) in relaxing solution (pCa 9.2) and decreased to 0.004 +/- 0.002 (+/- SEM, n = 9) at pCa 4.0. Force and dichroism had similar Ca2+ sensitivities. Force inhibition with BDM caused no change in the amplitude and Ca2+ sensitivity of dichroism. Similarly, inhibition of force at pCa 4.0 with 0.5 mM AlF4- decreased force to 0.04 +/- 0.01 of maximum (+/- SEM, n = 3), and dichroism was 0.04 +/- 0.03 (+/- SEM, n = 3) of the value at pCa 9.2 and unchanged relative to the corresponding normalized value at pCa 4.0 (0.11 +/- 0.05, +/- SEM; n = 3). Inhibition of force with AlF4- also had no effect when sTnC structure was monitored by labeling with either 5-dimethylamino-1-napthalenylsulfonylaziridine (DANZ) or 4-(N-(iodoacetoxy)ethyl-N-methyl)amino-7-nitrobenz-2-oxa-1,3-diazole (NBD). Increasing sarcomere length from 2.5 to 3.6 microm caused force (pCa 4.0) to decrease, but had no effect on dichroism. In contrast, rigor cross-bridge attachment caused dichroism at pCa 9.2 to decrease to 0.56 +/- 0.03 (+/- SEM, n = 5) of the value at pCa 9. 2, and force was 0.51 +/- 0.04 (+/- SEM, n = 6) of pCa 4.0 control. At pCa 4.0 in rigor, dichroism decreased further to 0.19 +/- 0.03 (+/- SEM, n = 6), slightly above the pCa 4.0 control level; force was 0.66 +/- 0.04 of pCa 4.0 control. These results indicate that cross-bridge binding in the rigor state alters sTnC structure, whereas cycling cross-bridges have little influence at either submaximum or maximum activating [Ca2+].  (+info)

Rational analyses of organelle trajectories in tobacco pollen tubes reveal characteristics of the actomyosin cytoskeleton. (4/982)

To gain insight into the characteristics of organelle movement and the underlying actomyosin motility system in tobacco pollen tubes, we collected data points representing sequential organelle positions in control and cytochalasin-treated cells, and in a sample of extruded cytoplasm. These data were utilized to reconstruct approximately 900 tracks, representing individual organelle movements, and to produce a quantitative analysis of the movement properties, supported by statistical tests. Each reconstructed track appeared to be unique and to show irregularities in velocity and direction of movement. The regularity quotient was near 2 at the tip and above 3 elsewhere in the cell, indicating that movement is more vectorial in the tube area. Similarly, the progressiveness ratio showed that there were relatively more straight trajectories in the tube region than at the tip. Consistent with these data, arithmetical dissection revealed a high degree of randomlike movement in the apex, lanes with tip-directed movement along the flanks, and grain-directed movement in the center of the tube. Intercalated lanes with bidirectional movement had lower organelle velocity, suggesting that steric hindrance plays a role. The results from the movement analysis indicate that the axial arrangement of the actin filaments and performance of the actomyosin system increases from tip to base, and that the opposite polarity of the actin filaments in the peripheral (+-ends of acting filaments toward the tip) versus the central cytoplasm (+-ends of actin filaments toward to the grain) is installed within a few minutes in these tip-growing cells.  (+info)

Alterations of cross-bridge kinetics in human atrial and ventricular myocardium. (5/982)

CONDENSED ABSTRACT: We analyzed actomyosin cross-bridge kinetics in human atrial and ventricular muscle strip preparations by using sinusoidal length changes from 0.1 to 60 Hz. The minimum stiffness frequency was higher in atrial than in ventricular human myocardium and lower in failing than in non-failing left ventricular human myocardium. beta-Adrenergic stimulation increased the minimum stiffness frequency by 18 +/- 3% (p < 0.05). Cross-bridge kinetics are temperature-dependent, with a Q10 of at least 2.7. BACKGROUND: Dynamic stiffness measurements have revealed acute and chronic alterations of actomyosin cross-bridge kinetics in cardiac muscles of a variety of different animal species. We studied dynamic stiffness in right atrial and left ventricular preparations of non-failing and failing human hearts and tested the influence of the temperature and beta-adrenergic stimulation on cross-bridge kinetics. METHODS AND RESULTS: Muscle strips were prepared from right atria and left ventricles from human non-failing and failing hearts. After withdrawal of calcium, steady contracture tension was induced by the addition of 1.5 mM barium chloride. Sinusoidal length oscillations of 1% muscle length were applied, with a frequency spectrum of between 0.1 and 60 Hz. Dynamic stiffness was calculated from the length change and the corresponding force response amplitude. The specific minimum stiffness frequency, which indicates the interaction between cross-bridge recruitment and cross-bridge cycling dynamics, was analyzed for each condition: (1) The minimum stiffness frequency was 0.78 +/- 0.04 Hz in left ventricular myocardium and 2.80 +/- 0.31 Hz in right atrial myocardium (p < 0.01) at 27 degrees C. (2) The minimum stiffness frequency was 41% higher in non-failing compared to failing left ventricular human myocardium. (3) Over a wide range of experimental temperatures, the minimum stiffness frequency changed, with a Q10 of at least 2.7. (4) beta-Adrenergic stimulation significantly (p < 0.05) increased the minimum stiffness to 18 +/- 3% higher frequencies and significantly (p < 0.05) lowered contracture tension by 7 +/- 1%. CONCLUSIONS: The contractility of human heart muscle is not only regulated by excitation-contraction coupling but also by modulation of intrinsic properties of the actomyosin system. Acute and chronic alterations of cross-bridge kinetics have been demonstrated, which play a significant role in the physiology and pathophysiology of the human heart.  (+info)

Amphidinolide B, a powerful activator of actomyosin ATPase enhances skeletal muscle contraction. (6/982)

Amphidinolide B caused a concentration-dependent increase in the contractile force of skeletal muscle skinned fibers. The concentration-contractile response curve for external Ca2+ was shifted to the left in a parallel manner, suggesting an increase in Ca2+ sensitivity. Amphidinolide B stimulated the superprecipitation of natural actomyosin. The maximum response of natural actomyosin to Ca2+ in superprecipitation was enhanced by it. Amphidinolide B increased the ATPase activity of myofibrils and natural actomyosin. The ATPase activity of actomyosin reconstituted from actin and myosin was enhanced in a concentration-dependent manner in the presence or absence of troponin-tropomyosin complex. Ca2+-, K+-EDTA- or Mg2+-ATPase of myosin was not affected by amphidinolide B. These results suggest that amphidinolide B enhances an interaction of actin and myosin directly and increases Ca2+ sensitivity of the contractile apparatus mediated through troponin-tropomyosin system, resulting in an increase in the ATPase activity of actomyosin and thus enhances the contractile response of myofilament.  (+info)

Backward movements of cross-bridges by application of stretch and by binding of MgADP to skeletal muscle fibers in the rigor state as studied by x-ray diffraction. (7/982)

The effects of the applied stretch and MgADP binding on the structure of the actomyosin cross-bridges in rabbit and/or frog skeletal muscle fibers in the rigor state have been investigated with improved resolution by x-ray diffraction using synchrotron radiation. The results showed a remarkable structural similarity between cross-bridge states induced by stretch and MgADP binding. The intensities of the 14.4- and 7.2-nm meridional reflections increased by approximately 23 and 47%, respectively, when 1 mM MgADP was added to the rigor rabbit muscle fibers in the presence of ATP-depletion backup system and an inhibitor for muscle adenylate kinase or by approximately 33 and 17%, respectively, when rigor frog muscle was stretched by approximately 4.5% of the initial muscle length. In addition, both MgADP binding and stretch induced a small but genuine intensity decrease in the region close to the meridian of the 5.9-nm layer line while retaining the intensity profile of its outer portion. No appreciable influence was observed in the intensities of the higher order meridional reflections of the 14.4-nm repeat and the other actin-based reflections as well as the equatorial reflections, indicating a lack of detachment of cross-bridges in both cases. The changes in the axial spacings of the actin-based and the 14.4-nm-based reflections were observed and associated with the tension change. These results indicate that stretch and ADP binding mediate similar structural changes, being in the correct direction to those expected for that the conformational changes are induced in the outer portion distant from the catalytic domain of attached cross-bridges. Modeling of conformational changes of the attached myosin head suggested a small but significant movement (about 10-20 degrees) in the light chain-binding domain of the head toward the M-line of the sarcomere. Both chemical (ADP binding) and mechanical (stretch) intervensions can reverse the contractile cycle by causing a backward movement of this domain of attached myosin heads in the rigor state.  (+info)

The translation in vitro of mRNA from developing cysts of Artemia salina. (8/982)

Successive stages in the development of the brine shrimp cyst were used as a model for studying differentiation at the level of mRNA transcription and translation. The poly (A)-containing mRNA from dormant cysts and free-swimming larvae (nauplii) was found to be efficiently translated in a wheat-germ cell-free system, and electrophoretic patterns of translation products in vitro resembled those of the endogenous proteins extracted from the equivalent developmental stages. Each stage, however, exhibits a characteristic protein pattern. Two low-molecular-weight proteins prominent in the cyst disappeared almost completely in the nauplius stage, whereas the proportion of actin increased 3-fold. Parallel patterns were observed upon translation in vitro of the respective mRNA preparations. The percentage of the acidic protein, tubulin, decreased somewhat during development.  (+info)

Unidirectional Brownian motion observed in an in silico single molecule experiment of an actomyosin motor<...
TY - JOUR. T1 - Unidirectional Brownian motion observed in an in silico single molecule experiment of an actomyosin motor. AU - Takano, Mitsunori. AU - Terada, Tomoki P.. AU - Sasai, Masaki. PY - 2010/4/27. Y1 - 2010/4/27. N2 - The actomyosin molecular motor, the motor composed of myosin II and actin filament, is responsible for muscle contraction, converting chemical energy into mechanical work. Although recent single molecule and structural studies have shed new light on the energy-converting mechanism, the physical basis of the molecular-level mechanism remains unclear because of the experimental limitations. To provide a clue to resolve the controversy between the lever-arm mechanism and the Brownian ratchet-like mechanism, we here report an in silico single molecule experiment of an actomyosin motor.When we placed myosin on an actin filament and allowed myosin to move along the filament, we found that myosin exhibits a unidirectional Brownian motion along the filament. This ...
https://waseda.pure.elsevier.com/en/publications/unidirectional-brownian-motion-observed-in-an-in-silico-single-mo
α-Catenin stabilises Cadherin-Catenin complexes and modulates actomyosin dynamics to allow pulsatile apical contraction -ORCAα-Catenin stabilises Cadherin-Catenin complexes and modulates actomyosin dynamics to allow pulsatile apical contraction -ORCA
We have investigated how cell contractility and adhesion are functionally integrated during epithelial morphogenesis. To this end, we have analysed the role of α-Catenin, a key molecule linking E-Cadherin-based adhesion and the actomyosin cytoskeleton, during Drosophila embryonic dorsal closure, by studying a newly developed allelic series. We find that α-Catenin regulates pulsatile apical contraction in the amnioserosa, the main force-generating tissue driving closure of the embryonic epidermis. α-Catenin controls actomyosin dynamics by stabilising and promoting the formation of actomyosin foci, and also stabilises DE-Cadherin (Drosophila E-Cadherin, also known as Shotgun) at the cell membrane, suggesting that medioapical actomyosin contractility regulates junction stability. Furthermore, we uncover a genetic interaction between α-Catenin and Vinculin, and a tension-dependent recruitment of Vinculin to amniosersoa apical cell membranes, suggesting the existence of a mechano-sensitive module ...
http://orca-mwe.cf.ac.uk/101732/
Mechanical measurements of single actomyosin motor force<...
TY - JOUR. T1 - Mechanical measurements of single actomyosin motor force. AU - Miyata, H.. AU - Yoshikawa, H.. AU - Hakozaki, H.. AU - Suzuki, N.. AU - Furuno, Taiji. AU - Ikegami, A.. AU - Kinosita, K.. AU - Nishizaka, T.. AU - Ishiwata, S.. AU - Driezen, P.. AU - Mehta, A.. PY - 1995. Y1 - 1995. N2 - To elucidate the mechanism of force generation by actomyosin motor, a measuring system was constructed, in which an in vitro motility assay was combined with an optical trapping technique. An actin filament of several μm long was attached to a gelsolin-coated polystyrene bead, and was allowed to interact with a small number (~1/1-μm actin filament) of rabbit skeletal heavy meromyosin (an active subfragment of myosin) molecules bound to a nitrocellulose-coated coverglass. The bead position was determined at 33-ms intervals. We measured the force generation event at relatively low (100-400 nM) ATP concentration so that the occurrence of individual force generation events could be detected with our ...
https://keio.pure.elsevier.com/en/publications/mechanical-measurements-of-single-actomyosin-motor-force
TRPM7, a novel regulator of actomyosin contractility and cell adhesion.TRPM7, a novel regulator of actomyosin contractility and cell adhesion.
Actomyosin contractility regulates various cell biological processes including cytokinesis, adhesion and migration. While in lower eukaryotes, alpha-kinases control actomyosin relaxation, a similar role for mammalian alpha-kinases has yet to be established. Here, we examined whether TRPM7, a cation channel fused to an alpha-kinase, can affect actomyosin function. We demonstrate that activation of TRPM7 by bradykinin leads to a Ca(2+)- and kinase-dependent interaction with the actomyosin cytoskeleton. Moreover, TRPM7 phosphorylates the myosin IIA heavy chain. Accordingly, low overexpression of TRPM7 increases intracellular Ca2+ levels accompanied by cell spreading, adhesion and the formation of focal adhesions. Activation of TRPM7 induces the transformation of these focal adhesions into podosomes by a kinase-dependent mechanism, an effect that can be mimicked by pharmacological inhibition of myosin II. Collectively, our results demonstrate that regulation of cell adhesion by TRPM7 is the combined ...
https://repository.ubn.ru.nl/handle/2066/51410
Welcome to CDC stacks | Contractile Units in Disordered Actomyosin Bundles Arise from F-Actin Buckling - 33757 | CDC Public...Welcome to CDC stacks | Contractile Units in Disordered Actomyosin Bundles Arise from F-Actin Buckling - 33757 | CDC Public...
Bundles of filaments and motors are central to contractility in cells. The classic example is striated muscle, where actomyosin contractility is mediated by highly organized sarcomeres which act as fundamental contractile units. However, many contractile bundles in vivo and in vitro lack sarcomeric organization. Here we propose a model for how contractility can arise in bundles without sarcomeric organization and validate its predictions with experiments on a reconstituted system. In the model, internal stresses in frustrated arrangements of motors with diverse velocities cause filaments to buckle, leading to overall shortening. We describe the onset of buckling in the presence of stochastic motor head detachment and predict that buckling-induced contraction occurs in an intermediate range of motor densities. We then calculate the size of the contractile units associated with this process. Consistent with these results, our reconstituted actomyosin bundles show contraction at relatively high ...
https://stacks.cdc.gov/view/cdc/33757
Bergen Open Research Archive: Assembly and positioning of actomyosin rings by contractility and planar cell polarityBergen Open Research Archive: Assembly and positioning of actomyosin rings by contractility and planar cell polarity
The actomyosin cytoskeleton is a primary force-generating mechanism in morphogenesis, thus a robust spatial control of cytoskeletal positioning is essential. In this report, we demonstrate that actomyosin contractility and planar cell polarity (PCP) interact in post-mitotic Ciona notochord cells to self-assemble and reposition actomyosin rings, which play an essential role for cell elongation. Intriguingly, rings always form at the cells′ anterior edge before migrating towards the center as contractility increases, reflecting a novel dynamical property of the cortex. Our drug and genetic manipulations uncover a tug-of-war between contractility, which localizes cortical flows toward the equator and PCP, which tries to reposition them. We develop a simple model of the physical forces underlying this tug-of-war, which quantitatively reproduces our results. We thus propose a quantitative framework for dissecting the relative contribution of contractility and PCP to the self-assembly and ...
https://bora.uib.no/bora-xmlui/handle/1956/11840
Iris Publication
Polarized epithelia develop distinct cell surface domains, with the apical membrane acquiring characteristic morphological features such as microvilli. Cell polarization is driven by polarity determinants including the evolutionarily conserved partitioning-defective (PAR) proteins that are separated into distinct cortical domains. PAR protein segregation is thought to be a consequence of asymmetric actomyosin contractions. The mechanism of activation of apically polarized actomyosin contractility is unknown. Here we show that the Cdc42 effector MRCK activates myosin-II at the apical pole to segregate aPKC-Par6 from junctional Par3, defining the apical domain. Apically polarized MRCK-activated actomyosin contractility is reinforced by cooperation with aPKC-Par6 downregulating antagonistic RhoA-driven junctional actomyosin contractility, and drives polarization of cytosolic brush border determinants and apical morphogenesis. MRCK-activated polarized actomyosin contractility is required for apical ...
https://iris.ucl.ac.uk/iris/publication/1414770/1
Thermal Inactivation of the Calcium Regulatory Mechanism of Human Skeletal Muscle Actomyosin:A Possible Contributing Factor in...Thermal Inactivation of the Calcium Regulatory Mechanism of Human Skeletal Muscle Actomyosin:A Possible Contributing Factor in...
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http://anesthesiology.pubs.asahq.org/article.aspx?articleid=1961396
Frontiers | Myosin IIA-related Actomyosin Contractility Mediates Oxidative Stress-induced Neuronal Apoptosis | Frontiers in...Frontiers | Myosin IIA-related Actomyosin Contractility Mediates Oxidative Stress-induced Neuronal Apoptosis | Frontiers in...
Oxidative stress-induced neuronal apoptosis plays an important role in the progression of central nervous system (CNS) diseases. In our study, when neuronal cells were exposed to hydrogen peroxide (H2O2), an exogenous oxidant, cell apoptosis was observed with typical morphological changes including membrane blebbing, neurite retraction and cell contraction. The actomyosin system is considered to be responsible for the morphological changes, but how exactly it regulates oxidative stress-induced neuronal apoptosis and the distinctive functions of different myosin II isoforms remain unclear. We demonstrate that myosin IIA was required for neuronal contraction, while myosin IIB was required for neuronal outgrowth in normal conditions. During H2O2-induced neuronal apoptosis, myosin IIA, rather than IIB, interacted with actin filaments to generate contractile forces that leaded to morphological changes. Moreover, myosin IIA knockout using CRISPR/Cas9 reduced H2O2-induced neuronal apoptosis and the associated
https://www.frontiersin.org/articles/10.3389/fnmol.2017.00075/full
Actomyosin contraction of the posterior hemisphere is required for inversion of the Volvox embryo | DevelopmentActomyosin contraction of the posterior hemisphere is required for inversion of the Volvox embryo | Development
During inversion of a Volvox embryo, a series of cell shape changes causes the multicellular sheet to bend outward, and propagation of the bend from the anterior to the posterior pole eventually results in an inside-out spherical sheet of cells. We use fluorescent and electron microscopy to study the behavior of the cytoskeleton in cells undergoing shape changes. Microtubules are aligned parallel to the cells long axis and become elongated in the bend. Myosin and actin filaments are arrayed perinuclearly before inversion. In inversion, actin and myosin are located in a subnuclear position throughout the uninverted region but this localization is gradually lost towards the bend. Actomyosin inhibitors cause enlargement of the embryo. The bend propagation is inhibited halfway and, as a consequence, the posterior hemisphere remains uninverted. The arrested posterior hemisphere will resume and complete inversion even in the presence of an actomyosin inhibitor if the anterior hemisphere is removed ...
http://dev.biologists.org/content/126/10/2117
Competition for actin between two distinct F-actin networks defines a bistable switch for cell polarization<...
TY - JOUR. T1 - Competition for actin between two distinct F-actin networks defines a bistable switch for cell polarization. AU - Lomakin, Alexis J.. AU - Lee, Kun Chun. AU - Han, Sangyoon J.. AU - Bui, Duyen A.. AU - Davidson, Michael. AU - Mogilner, Alex. AU - Danuser, Gaudenz. PY - 2015/11/1. Y1 - 2015/11/1. N2 - Symmetry-breaking polarization enables functional plasticity of cells and tissues and is yet not well understood. Here we show that epithelial cells, hard-wired to maintain a static morphology and to preserve tissue organization, can spontaneously switch to a migratory polarized phenotype after relaxation of the actomyosin cytoskeleton. We find that myosin II engages actin in the formation of cortical actomyosin bundles and thus makes it unavailable for deployment in the process of dendritic growth normally driving cell motility. Under low-contractility regimes, epithelial cells polarize in a front-back manner owing to the emergence of actin retrograde flows powered by dendritic ...
https://ucdavis.pure.elsevier.com/en/publications/competition-for-actin-between-two-distinct-f-actin-networks-defin-2
Tissue mechanical properties modulate cell extrusion in the Drosophila abdominal epidermis | DevelopmentTissue mechanical properties modulate cell extrusion in the Drosophila abdominal epidermis | Development
The replacement of cells is a common strategy during animal development. In the Drosophila pupal abdomen, larval epidermal cells (LECs) are replaced by adult progenitor cells (histoblasts). Previous work showed that interactions between histoblasts and LECs result in apoptotic extrusion of LECs during early pupal development. Extrusion of cells is closely preceded by caspase activation and is executed by contraction of a cortical actomyosin cable. Here, we identify a population of LECs that extrudes independently of the presence of histoblasts during late pupal development. Extrusion of these LECs is not closely preceded by caspase activation, involves a pulsatile medial actomyosin network, and correlates with a developmental time period when mechanical tension and E-cadherin turnover at adherens junctions is particularly high. Our work reveals a developmental switch in the cell extrusion mechanism that correlates with changes in tissue mechanical properties. ...
https://dev.biologists.org/content/147/5/dev179606
Microtubules tune mechanosensitive cell responses - Institut PasteurMicrotubules tune mechanosensitive cell responses - Institut Pasteur
Mechanotransduction is a process by which cells sense the mechanical properties of their surrounding environment and adapt accordingly to perform cellular functions such as adhesion, migration and differentiation. Integrin-mediated focal adhesions are major sites of mechanotransduction and their connection with the actomyosin network is crucial for mechanosensing as well as the generation and transmission of forces onto the substrate. Despite having emerged as major regulators of cell adhesion and migration, the contribution of microtubules to mechanotransduction still remains elusive. Here, we show that actomyosin-dependent mechanosensing of substrate rigidity controls microtubule acetylation, a tubulin post-translational modification, by promoting the recruitment of the alpha-tubulin acetyl transferase (αTAT1) to focal adhesions. Microtubule acetylation, in turn, promotes GEF-H1 mediated RhoA activation, actomyosin contractility and traction forces. Our results reveal a fundamental crosstalk between
https://hal-pasteur.archives-ouvertes.fr/pasteur-03096554
Head involution in Drosophila melanogaster: on the role of supracellular actomyosin structures in tissue bending, spreading,...Head involution in Drosophila melanogaster: on the role of supracellular actomyosin structures in tissue bending, spreading,...
La morfogènesi crea una plètora de formes complexes en animals i plantes. Hem consagrat aquest treball a lestudi de la involució del cap (head involution HI) de Drosophila, un procés embriogenètic tardiu, que implica un complet rearranjament dels teixits del cap, així com la internalització del cervell i la propagació de lepidermis. Mostrem, pel primer cop, la cinètica completa de HI amb una alta resolució espacial i temporal. Describim els moviments que porten a la internalització del cervell de lembrió, així com el seu sculpting per apoptosi i leliminació de cèl.lules pels hemòcits. Seguidament, hem enfocat lestudi en la progressió de lepidermis sobre el cap de lembrió, essent aquest un esdeveniment que es pot dividir en dues fases: rodolament i lliscament. Mostrem que totes dues fases son impulsades per un cable dactomyosina. També mostrem que la propagació de lepidermis es troba espacialment controlada, tenint aquest control com a resultat la formació de ...
https://tesisenred.net/handle/10803/386441
Characterisation of the expression profile and endothelial function of Rho GTPase RhoJ - eTheses RepositoryCharacterisation of the expression profile and endothelial function of Rho GTPase RhoJ - eTheses Repository
Rho GTPases are molecular switches that regulate many aspects of cell physiology. A number of Rho GTPases are essential for the formation of new vessels from pre-existing ones, a process known as angiogenesis. RhoJ/TCL belongs to the Cdc42 subfamily of Rho GTPases. Previous bioinformatic and primary cell line analyses identified RhoJ as being highly expressed in endothelial cells. The aim of this project was to investigate the expression pattern and endothelial function of RhoJ, particularly in the processes necessary for angiogenesis. Silencing RhoJ with siRNA impaired tube formation and migration. On the cellular level, RhoJ knockdown increased focal adhesions, actin stress fibres and collagen gel contraction, suggesting increased actomyosin contractility. Pharmacological inhibition of ROCK and myosin II, two regulators of actomyosin contractility, restored motility and tube formation after RhoJ knockdown. RhoJ localised to blood vessels of developing mice and in various human normal and ...
http://etheses.bham.ac.uk/1558/
Interactive Fly, DrosophilaInteractive Fly, Drosophila
The wound healing response is an essential mechanism to maintain the integrity of epithelia and protect all organisms from the surrounding milieu. In the purse-string mechanism of wound closure, an injured epithelial sheet cinches its hole closed via an intercellular contractile actomyosin cable. This process is conserved across species and utilized by both embryonic as well as adult tissues, but remains poorly understood at the cellular level. In an effort to identify new players involved in purse-string wound closure a wounding strategy suitable for screening large numbers of Drosophila embryos was developed. Using this methodology, wound healing defects were observed in Jun-related antigen (encoding DJUN) and scab (encoding Drosophila alphaPS3 integrin) mutants and a forward genetics screen was performed on the basis of insertional mutagenesis by transposons that led to the identification of 30 lethal insertional mutants with defects in embryonic epithelia repair. One of the mutants ...
http://www.sdbonline.org/sites/fly/torstoll/basktjk5.htm
Misshapen coordinates protrusion restriction and actomyosin contractility during collective cell migration. | IRIC - Institute...Misshapen coordinates protrusion restriction and actomyosin contractility during collective cell migration. | IRIC - Institute...
Collective cell migration is involved in development, wound healing and metastasis. In the Drosophila ovary, border cells (BC) form a small cluster that migrates collectively through the egg chamber. To achieve directed motility, the BC cluster coordinates the formation of protrusions in its leader cell and contractility at the rear. Restricting protrusions to leader cells requires the actin and plasma membrane linker Moesin. Herein, we show that the Ste20-like kinase Misshapen phosphorylates Moesin in vitro and in BC. Depletion of Misshapen disrupts protrusion restriction, thereby allowing other cells within the cluster to protrude. In addition, we show that Misshapen is critical to generate contractile forces both at the rear of the cluster and at the base of protrusions. Together, our results indicate that Misshapen is a key regulator of BC migration as it coordinates two independent pathways that restrict protrusion formation to the leader cells and induces contractile forces.. ...
https://www.iric.ca/en/research/publications/misshapen-coordinates-protrusion-restriction-and-actomyosin-contractility-during-collective-cell-migration
NDLI: Actomyosin Contractility Rotates The Cell NucleusNDLI: Actomyosin Contractility Rotates The Cell Nucleus
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access ...
https://ndl.iitkgp.ac.in/document/SEpXamVlRGV1RkEraWtXQ1RrcThWeEd4SWJTSFVRMFAxMkpkTjJLWm9WWT0
KLUEDO | 92B05 General biology and biomathematicsKLUEDO | 92B05 General biology and biomathematics
Cell migration is essential for embryogenesis, wound healing, immune surveillance, and progression of diseases, such as cancer metastasis. For the migration to occur, cellular structures such as actomyosin cables and cell-substrate adhesion clusters must interact. As cell trajectories exhibit a random character, so must such interactions. Furthermore, migration often occurs in a crowded environment, where the collision outcome is deter- mined by altered regulation of the aforementioned structures. In this work, guided by a few fundamental attributes of cell motility, we construct a minimal stochastic cell migration model from ground-up. The resulting model couples a deterministic actomyosin contrac- tility mechanism with stochastic cell-substrate adhesion kinetics, and yields a well-defined piecewise deterministic process. The signaling pathways regulating the contractility and adhesion are considered as well. The model is extended to include cell collectives. Numer- ical simulations of single ...
https://kluedo.ub.uni-kl.de/solrsearch/index/search/searchtype/collection/id/13510
Intestinal permeability (Cyrex array 2) - Embracing Nutrition & Functional MedicineIntestinal permeability (Cyrex array 2) - Embracing Nutrition & Functional Medicine
Zonulin and occulin are proteins that open up the tight junctions of the intestinal wall, regulating how permeable the gut is. Researchers first discovered zonulin while studying intestinal infection cholera. It has since been shown that gluten can trigger zonulin release and cause the gut to become more permeable in people reactive to gluten.. As the gut becomes inflamed and breaks down during leaky gut, the immune system makes antibodies to zonulin and occludin. One way to evaluate leaky gut is to test for elevated zonulin-occludin antibodies.. Actomyosin antibodies are an indicator of intestinal destruction. Actomyosin is a complex of proteins that make up muscle fibres and contributes to contractions in the intestine. Antibodies to actomyosin signal a breakdown of the membrane lining the digestive tract and hence leaky gut.. Actomyosin antibodies indicate gut damage is severe enough to break through the cells, not just open the spaces between cells. This type of damage takes longer to ...
https://embracingnutrition.co.uk/shop/tests/intestinal-permeability-cyrex-array-2/
Discovery of α-N-trimethylalanine in myosin light chains and its role in actomyosin interaction | Biochemical Society...Discovery of α-N-trimethylalanine in myosin light chains and its role in actomyosin interaction | Biochemical Society...
Thank you for your interest in spreading the word about Biochemical Society Transactions.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
http://www.biochemsoctrans.org/content/10/5/362
An abnormal adherence of monocytes to fibronectin in thyroid autoimmunity has consequences for cell polarization and the...An abnormal adherence of monocytes to fibronectin in thyroid autoimmunity has consequences for cell polarization and the...
Blood monocytes of patients with thyroid autoimmune disease (TAID) display defects in rearranging their cortical actomyosin cytoskeleton (polarize) in response to chemoattractants. Such rearrangements also take place after the adherence of monocytes to the extracellular matrix (ECM). It is therefo …
https://pubmed.ncbi.nlm.nih.gov/11472420/?dopt=Abstract
Spatial localisation of actin filaments across developmental stages of the malaria parasiteSpatial localisation of actin filaments across developmental stages of the malaria parasite
Actin dynamics have been implicated in a variety of developmental processes during the malaria parasite lifecycle. Parasite motility, in particular, is thought to critically depend on an actomyosin motor located in the outer pellicle of the parasite cell. Efforts to understand the diverse roles acti …
https://pubmed.ncbi.nlm.nih.gov/22389687/?dopt=Abstract
Frmd6 - FERM domain-containing protein 6 - Mus musculus (Mouse) - Frmd6 gene & proteinFrmd6 - FERM domain-containing protein 6 - Mus musculus (Mouse) - Frmd6 gene & protein
apical junction complex, cytoskeleton, structural constituent of cytoskeleton, actomyosin structure organization, apical constriction, cellular protein localization, regulation of actin filament-based process
http://www.uniprot.org/uniprot/Q8C0V9
Background Cellular contractility, important for cell proliferation and movement, is certainly - Small Molecule Inhibitors of...Background Cellular contractility, important for cell proliferation and movement, is certainly - Small Molecule Inhibitors of...
Background Cellular contractility, important for cell proliferation and movement, is certainly controlled by microtubules, Actomyosin and RhoA. natural phenomena such as cytokinesis, motion, difference and substrate adherence. These obvious adjustments in cell form are accomplished by modulation of the cytoskeleton, most the actin cytoskeleton significantly, through pushes produced by the actomyosin network. This network can be controlled primarily through the activity of aminoacids from the Rho-GTPase family members that regulate both actin nucleation and myosin activity through downstream effectors such as mDia and Rock and roll (Rho kinase), respectively (evaluated by [1], [2], [3]). Cell contractility can be typically accomplished by localised service of Myosin II Light String (MLC) by its phosphorylation on Ser19. This phosphorylation, which causes contractions of the actin network by Myosin II is regulated by various MLC kinases and Myosin phosphatase (MYP). This 329932-55-0 site is ...
https://welbourneprimary.com/background-cellular-contractility-important-for-cell-proliferation-and-movement-is-certainly/
New type of protein-protein interactionNew type of protein-protein interaction
New type of protein-protein interaction: actomyosin gels interact in a THRESHOLD manner. For details see the following paper: V.V.Matveev. Evidence of a new type of protein-protein interaction: desensitized actomyosin blocks Ca2+-sensitivity of the natural one. A possible model for an intracellular signalling system related to actin filaments. Physiological Chemistry Physics & Medical NMR, 32: 167-179, 2000. ABSTRACT see here: http://members.nbci.com/vm_spb/actomyosin/signal.htm Sent via Deja.com http://www.deja.com/ Before you buy ...
http://www.bio.net/bionet/mm/immuno/2000-November/016214.html
Cooperation Between the Septins and the Actomyosin Ring and Role of a Cell-integrity Pathway During Cell Division in Fission...Cooperation Between the Septins and the Actomyosin Ring and Role of a Cell-integrity Pathway During Cell Division in Fission...
The Genetics Society of America (GSA), founded in 1931, is the professional membership organization for scientific researchers and educators in the field of genetics. Our members work to advance knowledge in the basic mechanisms of inheritance, from the molecular to the population level.. Online ISSN: 1943-2631. ...
http://www.genetics.org/content/early/2010/08/25/genetics.110.119842
Find people in the EMBO Communities
My group develops and uses advanced imaging technologies (e.g., photoactivation, FRAP, single particle tracking, multispectral unmixing and PALM) to study the complexity of cell behaviors (e.g., cell crawling, polarity, cytokinesis, viral budding and intercellular transfer) and dynamism of subcellular organelles (e.g., ER, mitochondria, Golgi, endosomes, lysosomes, actomyosin filaments, microtubules, autophagosomes, peroxisomes and cilia) under both healthy and pathological conditions.. Keywords: Organelle dynamics / ER / mitochondria / Golgi / peroxisomes / lipid droplets / actin / secretory pathway / autophagy / super resolution imaging / multispectral imaging / single molecule tracking / HIV budding / ESCRTs. Subject area(s): Membranes & Transport , Proteins & Biochemistry , Structural Biology & Biophysics. ...
https://people.embo.org/profile/jennifer-lippincott-schwartz
Anti-PPP1R12A AntibodyAnti-PPP1R12A Antibody
Polyclonal antibody for PPP1R12A detection. Host: Rabbit.Size: 100μg/vial. Tested applications: Flow Cytometry. Reactive species: Human. PPP1R12A information: Molecular Weight: 115281 MW; Subcellular Localization: Cytoplasm . Along actomyosin filaments an
https://www.bosterbio.com/anti-ppp1r12a-antibody-pa1681-boster.html
OPUS 4 | SearchOPUS 4 | Search
A wide variety of cell types exhibit substrate topography-based behavior, also known as contact guidance. However, the precise cellular mechanisms underlying this process are still unknown. In this study, we investigated contact guidance by studying the reaction of human endothelial cells (ECs) to well-defined microgroove topographies, both during and after initial cell spreading. As the cytoskeleton plays a major role in cellular adaptation to topographical features, two methods were used to perturb cytoskeletal structures. Inhibition of actomyosin contractility with the chemical inhibitor blebbistatatin demonstrated that initial contact guidance events are independent of traction force generation. However, cell alignment to the grooved substrate was altered at later time points, suggesting an initial passive phase of contact guidance, followed by a contractility-dependent active phase that relies on mechanosensitive feedback. The actin cytoskeleton was also perturbed in an indirect manner ...
https://publikationen.reutlingen-university.de/solrsearch/index/search/searchtype/authorsearch/author/%22Kemkemer%2C+Ralf%22/start/0/rows/100/facetNumber_publisher_name_facet/all/sortfield/year/sortorder/desc/yearfq/2017/author_facetfq/Sales%2C+Adria/publisher_name_facetfq/Royal+Society+of+Chemistry
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Kumar, Abhishek and Maitra, Ananyo and Sumit, Madhuresh and Ramaswamy, Sriram and Shivashankar, G. V. (2014) Actomyosin contractility rotates the cell nucleus. In: SCIENTIFIC REPORTS, 4 . ...
http://eprints.iisc.ac.in/view/people/Sumit=3AMadhuresh=3A=3A.html
The protein model of signal transductionThe protein model of signal transduction
The protein model of signal transduction system based on actin cytoskeleton. V.V.Matveev. Evidence of a new type of protein-protein interaction: desensitized actomyosin blocks Ca2+-sensitivity of the natural one. A possible model for an intracellular signalling system related to actin filaments. Physiological Chemistry Physics & Medical NMR, 32: 167-179, 2000. ABSTRACT see here: http://members.nbci.com/vm_spb/actomyosin/signal.htm Sent via Deja.com http://www.deja.com/ Before you buy ...
http://www.bio.net/bionet/mm/immuno/2000-December/016258.html
1703.04395] Maximal fluctuations of confined actomyosin gels: dynamics of the cell nucleus1703.04395] Maximal fluctuations of confined actomyosin gels: dynamics of the cell nucleus
Abstract: We investigate the effect of stress fluctuations on the stochastic dynamics of an inclusion embedded in a viscous gel. We show that, in non-equilibrium systems, stress fluctuations give rise to an effective attraction towards the boundaries of the confining domain, which is reminiscent of an active Casimir effect. We apply this generic result to the dynamics of deformations of the cell nucleus and we demonstrate the appearance of a fluctuation maximum at a critical level of activity, in agreement with recent experiments [E. Makhija, D. S. Jokhun, and G. V. Shivashankar, Proc. Natl. Acad. Sci. U.S.A. 113, E32 (2016 ...
http://export.arxiv.org/abs/1703.04395?context=cond-mat
S pombe Rng2 protein Summary Report | CureHunterS pombe Rng2 protein Summary Report | CureHunter
S pombe Rng2 protein: a component of the actomyosin ring and the spindle pole body; homologous to S cerevisiae IQG1 protein; amino acid sequence in first source
http://www.curehunter.com/public/keywordSummaryC114172-S-pombe-Rng2-protein.do
The cell cortex | Charras LabThe cell cortex | Charras Lab
What is actomyosin cortex? The cell cortex is a thin (~0.1 µm thick) network of actin filaments and actin-binding proteins that underlies the plasma membrane in most eukaryotic cells. The cortex is the main determinant of cell shape and therefore plays a fundamental role in processes such as cell division, migration, and tissue morphogenesis. Despite…
https://charraslab.com/the-actin-cortex/
LEFT OVER LIGHT by jacques seronde (Paperback) - LuluLEFT OVER LIGHT by jacques seronde (Paperback) - Lulu
Buy LEFT OVER LIGHT by jacques seronde (Paperback) online at Lulu. Visit the Lulu Marketplace for product details, ratings, and reviews.
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HER LIGHT by Thomas Weatherspoon (Paperback) - LuluHER LIGHT by Thomas Weatherspoon (Paperback) - Lulu
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http://www.lulu.com/shop/thomas-weatherspoon/her-light/paperback/product-5571283.html
Correlation between myosin heavy chain expression and actomyosin atpase activity in human muscle fibers<...
TY - JOUR. T1 - Correlation between myosin heavy chain expression and actomyosin atpase activity in human muscle fibers. AU - Proctor, David Nathan. AU - Joyner, M. J.. AU - Sieck, G. C.. PY - 1996. Y1 - 1996. N2 - The myosin heavy chain (MHC) is the site of ATP hydrolysis during crossbridge cycling; therefore, a correlation should exist between MHC isoform expression and actomyosin ATPase activity in human muscle fibers. Needle biopsies were obtained from the quadriceps femoris of 5 sedentary subjects (21-43 yr). Fibers were classified histochemicalîy and also on the basis of MHC isoform immunoreactivity. Calcium-activated actomyosin ATPase activity of muscle fibers (20 fibers per type) was determined in alternate serial sections using a quantitative histochemical procedure (Blanco & Sieck, Histochem J. 24: 431-444, 1992}. The mean actomyosin ATPase activity (mmol Pi/ liter tissue/min) of type I fibers (52.8±6.1) was two-fold lower (p,0.05) than that of type Ha (102.1 + 13.3) and type IIx ...
https://pennstate.pure.elsevier.com/en/publications/correlation-between-myosin-heavy-chain-expression-and-actomyosin-
Lecture | Page 7 | Tartu Ãœlikooli molekulaar- ja rakubioloogia instituutLecture | Page 7 | Tartu Ãœlikooli molekulaar- ja rakubioloogia instituut
Prof Pekka Lappalainen, Helsingi Ãœlikooli Biotehnoloogia Instituudi teadusdirektor. Seminari teema: Assembly of contractile actomyosin bundles in migrating cells. Seminari toetab Biomeditsiini ja biotehnoloogia doktorikool ASTRA meetme kaudu (Euroopa Liidu Regionaalarengu Fond). ...
https://www.tymri.ut.ee/et/event-category/lecture?page=6
MAGIs regulate aPKC to enable balanced distribution of intercellular tension for epithelial sheet homeostasis<...
TY - JOUR. T1 - MAGIs regulate aPKC to enable balanced distribution of intercellular tension for epithelial sheet homeostasis. AU - Matsuzawa, Kenji. AU - Ohga, Hayato. AU - Shigetomi, Kenta. AU - Shiiya, Tomohiro. AU - Hirashima, Masanori. AU - Ikenouchi, Junichi. N1 - Publisher Copyright: © 2021, The Author(s).. PY - 2021/12. Y1 - 2021/12. N2 - Constriction of the apical plasma membrane is a hallmark of epithelial cells that underlies cell shape changes in tissue morphogenesis and maintenance of tissue integrity in homeostasis. Contractile force is exerted by a cortical actomyosin network that is anchored to the plasma membrane by the apical junctional complexes (AJC). In this study, we present evidence that MAGI proteins, structural components of AJC whose function remained unclear, regulate apical constriction of epithelial cells through the Par polarity proteins. We reveal that MAGIs are required to uniformly distribute Partitioning defective-3 (Par-3) at AJC of cells throughout the ...
https://kyushu-u.pure.elsevier.com/en/publications/magis-regulate-apkc-to-enable-balanced-distribution-of-intercellu
Terminal web - Biology-Online DictionaryTerminal web - Biology-Online Dictionary
The terminal web is a zone or a region located at the base of the microvilli in certain specialized epithelial cells (such as intestinal epithelial cells). The name is derived from the web (meshwork) of microfilaments that compose this region. The web of microfilaments is from the bundles of apical filaments at the core of a microvillus as well as from adherens junctions in myosin and in other proteins characteristic of an actomyosin motor system. The actin filaments in the terminal web are stabilized by spectrin. They anchor the terminal web to the apical cell membrane. The contractile ability of the terminal web is due to the presence of myosin II and tropomyosin. It is observed that the contraction at the terminal web results in the decrease in the diameter of the apex of the cell. This, in turn, causes the microvilli to spread apart, which is essential during absorption.1,2,3 ...
https://www.biology-online.org/dictionary/Terminal_web
YAP1 gene - Genetics Home Reference - NIHYAP1 gene - Genetics Home Reference - NIH
Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).. Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637, PubMed:30447097). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). Plays a key role to ...
https://ghr.nlm.nih.gov/gene/YAP1
Cytokinesis in development and disease: Variations on a common theme<...
TY - JOUR. T1 - Cytokinesis in development and disease. T2 - Variations on a common theme. AU - Li, R.. PY - 2007/12. Y1 - 2007/12. N2 - Cytokinesis is a crucial step in cell proliferation, and remarkably, it is also an important mechanism for developmental regulation in the generation of diverse cell types in eukaryotic organisms. Successful cytokinesis relies on the assembly and activation of an actomyosin-based contractile ring and membrane deposition/fusion in a spatially and temporally precise manner. As such, the molecular pathways governing cytokinesis are highly complex, involving a large number of components forming intricate interactive networks. The complexity of this system, however, may have also provided a rich platform for evolutionary tinkering to achieve specific morphogenetic and developmental outcomes. Furthermore, failed or altered cytokinesis appears to contribute to the development of cancer in unexpected ways.. AB - Cytokinesis is a crucial step in cell proliferation, ...
https://jhu.pure.elsevier.com/en/publications/cytokinesis-in-development-and-disease-variations-on-a-common-the-3
Diabetes: new hope for better wound healingDiabetes: new hope for better wound healing
Parisa Kakanj, the author of the study, examined the skin of larvae of the fruit fly Drosophila melanogaster. These flies serve as models for diabetes, because insulin metabolism has been strongly conserved over the course of evolution, meaning that flies and mammals are very similar in this respect. Using a precision laser, Kakanj removed a cell from the outermost skin layer of fruit fly larvae and then observed what happens in the neighbouring cells live under the microscope.. Immediately after a skin injury, the neighbouring cells respond by forming an actomyosin cable, Kakanj explains. The cable consists of proteins that otherwise occur in muscle fibres, where they are responsible for muscular contraction. After an injury, the cable forms a contractile ring around the wound. It then contracts, sealing off the gap caused by the wound. However, if insulin metabolism is impaired, as in our genetically modified flies, the cable is weaker and forms much later. This results in incomplete or ...
http://www.innovations-report.com/html/reports/life-sciences/diabetes-new-hope-for-better-wound-healing.html
Epithelial repair is a two-stage process driven first by dying cells and then by their neighbours | Journal of Cell ScienceEpithelial repair is a two-stage process driven first by dying cells and then by their neighbours | Journal of Cell Science
Epithelial cells maintain an essential barrier despite continuously undergoing mitosis and apoptosis. Biological and biophysical mechanisms have evolved to remove dying cells while maintaining that barrier. Cell extrusion is thought to be driven by a multicellular filamentous actin ring formed by neighbouring cells, the contraction of which provides the mechanical force for extrusion, with little or no contribution from the dying cell. Here, we use live confocal imaging, providing time-resolved three-dimensional observations of actomyosin dynamics, to reveal new mechanical roles for dying cells in their own extrusion from monolayers. Based on our observations, the clearance of dying cells can be subdivided into two stages. The first, previously unidentified, stage is driven by the dying cell, which exerts tension on its neighbours through the action of a cortical contractile F-actin and myosin ring at the cell apex. The second stage, consistent with previous studies, is driven by a multicellular ...
https://jcs.biologists.org/content/127/6/1229.abstract
The tiny GTPase Rac1 continues to be implicated in the dissemination - HDAC Inhibition for the Disruption of Latent HIV-1...The tiny GTPase Rac1 continues to be implicated in the dissemination - HDAC Inhibition for the Disruption of Latent HIV-1...
The tiny GTPase Rac1 continues to be implicated in the dissemination and formation of tumours. and invasion play a significant role in a variety of physiological functions such as for example embryonic development immune system cell replies and wound recovery. Furthermore aberrant legislation of cell motility is certainly associated with tumour progression with an increase of cell migration and invasion marking an integral part of metastasis the main cause of loss of life in cancer sufferers1 2 Research centered on deciphering the root mechanisms involved with cell migration and invasion possess identified five crucial guidelines that govern the mesenchymal-mode of single-cell motility: (1) front-rear polarization; (2) membrane protrusion on the industry leading; (3) cell-extra-cellular matrix (ECM) adhesion; (4) actomyosin contractility; and (5) detachment from the cell back3 4 Provided the mandatory interplay between your actin cytoskeleton cell-ECM adhesions and myosin electric motor ...
https://recyclers-info.com/the-tiny-gtpase-rac1-continues-to-be-implicated-in-the-dissemination/
Specificities of β1 integrin signaling in the control of cell adhesion and adhesive strength. - Inserm
Cells exert actomyosin contractility and cytoskeleton-dependent force in response to matrix stiffness cues. Cells dynamically adapt to force by modifying their behavior and remodeling their microenvironment. This adaptation is favored by integrin activation switch and their ability to modulate their clustering and the assembly of an intracellular hub in response to force. Indeed integrins are mechanoreceptors and mediate mechanotransduction by transferring forces to specific adhesion proteins into focal adhesions which are sensitive to tension and activate intracellular signals. α(5)β(1) integrin is considered of major importance for the formation of an elaborate meshwork of fibronectin fibrils and for the extracellular matrix deposition and remodeling. Here we summarize recent progress in the study of mechanisms regulating the activation cycle of β(1) integrin and the specificity of α(5)β(1) integrin in mechanotransduction.
https://www.hal.inserm.fr/inserm-00544923
Nature - June 2014
Malignancy is associated with altered expression of glycans and glycoproteins that contribute to the cellular glycocalyx. We constructed a glycoprotein expression signature, which revealed that metastatic tumors upregulate expression of bulky glycoproteins. A computational model predicted that these glycoproteins would influence transmembrane receptor spatial organization and function. We tested this prediction by investigating whether a bulky glycocalyx promotes a tumor phenotype by increasing integrin adhesion and signaling. Data revealed that a bulky glycocalyx facilitates integrin clustering by funneling active integrins into adhesions and altering integrin state by applying tension to matrix-bound integrins, independent of actomyosin contractility. Expression of large tumor-associated glycoproteins in non-transformed mammary cells promoted focal adhesion assembly and facilitated integrin-dependent growth factor signaling to support cell growth and survival. Clinical studies revealed that ...
http://www.iins.u-bordeaux.fr/Nature-June-2014-233
Frontiers | MYBPC1, an Emerging Myopathic Gene: What We Know and What We Need to Learn | PhysiologyFrontiers | MYBPC1, an Emerging Myopathic Gene: What We Know and What We Need to Learn | Physiology
Myosin Binding Protein-C (MyBP-C) comprises a family of accessory proteins that includes the cardiac, slow skeletal, and fast skeletal isoforms. The three isoforms share structural and sequence homology, and localize at the C-zone of the sarcomeric A-band where they interact with thick and thin filaments to regulate the cycling of actomyosin crossbridges. The cardiac isoform, encoded by MYBPC3, has been extensively studied over the last several decades due to its high mutational rate in congenital hypertrophic and dilated cardiomyopathy. It is only recently, however, that the MYBPC1 gene encoding the slow skeletal isoform (sMyBP-C) has gained attention. Accordingly, during the last five years it has been shown that MYBPC1 undergoes extensive exon shuffling resulting in the generation of multiple slow variants, which are co-expressed in different combinations and amounts in both slow and fast skeletal muscles. The sMyBP-C variants are subjected to PKA- and PKC-mediated phosphorylation in constitutive and
https://www.frontiersin.org/articles/10.3389/fphys.2016.00410/full
Kupata Hazina Katika Mji wa LuluKupata Hazina Katika Mji wa Lulu
Kupata Hazina Katika Mji wa Lulu. NA MWANDISHI WA AMKENI! NCHINI AUSTRALIA. BROOME ni mji ambao uko kaskazini-magharibi mwa Australia na umezingirwa na ufuko wa bahari. Jangwa Kuu la Mchanga limeanzia upande wa kusini-mashariki wa mji huo na kuelekea katikati ya Australia. Bahari ya Hindi iko upande wa magharibi wa mji huo, nayo inaelekea hadi pwani ya Afrika. Tufani huvuma mara nyingi kwenye sehemu hiyo iliyoko kaskazini-magharibi mwa bara hilo.. Hapo zamani, kulikuwa na chaza wengi wenye lulu katika bahari ya eneo hilo la kitropiki hivi kwamba Broome uliitwa Mji wa Lulu. Eneo la Broome lilitembelewa na maharamia, watumwa, na wauzaji wa lulu.. Uvumbuzi wa Haramia. Ijapokuwa Mholanzi Dirck Hartog alitembelea eneo hilo la mbali katika mwaka wa 1616, pwani ya magharibi ya Australia haikujulikana sana hadi mwaka wa 1688. Katika mwaka huo, Mwingereza William Dampier, aliyekuwa mwandishi, mchoraji, na haramia, alifika kwenye pwani hiyo bila kutarajia. Alikuwa akisafiri katika meli yake ya maharamia ...
https://www.jw.org/sw/maktaba/magazeti/g20020408/Kupata-Hazina-Katika-Mji-wa-Lulu/
QuimP | BISEQuimP | BISE
Summary. QuimP is software for tracking cellular shape changes and dynamic distributions of fluorescent reporters at the cell membrane. QuimPs unique selling point is the possibility to aggregate data from many cells in form of spatio-temporal maps of dynamic events, independently of cell size and shape. QuimP has been successfully applied to address a wide range of problems related to cell movement in many different cell types. Introduction. In transmembrane signalling the cell membrane plays a fundamental role in localising intracellular signalling components to specific sites of action, for example to reorganise the actomyosin cortex during cell polarisation and locomotion. The localisation of different components can be directly or indirectly visualised using fluorescence microscopy, for high-throughput screening commonly in 2D. A quantitative understanding demands segmentation and tracking of whole cells and fluorescence signals associated with the moving cell boundary, for example those ...
http://biii.eu/quimp
Working model of vertex ring assembly. See text for det | Open-iWorking model of vertex ring assembly. See text for det | Open-i
Working model of vertex ring assembly. See text for details. Solid black arrows indicate a strict hierarchical requirement for vertex enrichment. Dotted arrows
https://openi.nlm.nih.gov/detailedresult.php?img=PMC2172665_200209095f8&req=4
Pengumuman Final Lulus Beasiswa Mentoring Flutter Intermediate Batch 4 - UDACODINGPengumuman Final Lulus Beasiswa Mentoring Flutter Intermediate Batch 4 - UDACODING
Bismillah,. Ucapan terima kasih kami sampaikan kepada seluruh peserta yang telah antusias mendaftar pada Program Beasiswa Mentoring Intermediate batch 4 Udacoding khususnya bidang Flutter.. Setelah melalui rangkaian proses seleksi, kami ucapkan Selamat kepada para peserta yang LULUS. Dengan ini, Anda berhak mengikuti Program Mentoring Udacoding (level intermediate) dengan 100% Free. Berikut kami lampirkan Nama-nama Peserta yang LULUS Flutter Intermediate batch 4 ini : ...
https://www.udacoding.com/pengumuman-final-lulus-beasiswa-mentoring-flutter-intermediate-batch-4/
Actomyosin ringActomyosin ring
It is composed of actin and myosin II bundles, thus the term actomyosin. The actomyosin ring operates in contractile motion, ... 2016). Actomyosin ring driven cytokinesis in budding yeast. Seminars in Cell & Developmental Biology 53:19-27. Alberts, B., A. ... In plant cells, there is no actomyosin ring. Instead, a cell plate grows centrifugally outwards from the center of the plane of ... 2016). Actomyosin Ring Formation and Tension Generation in Eukaryotic Cytokinesis. Curr Biol. 26(15):719-737. Mana-Capelli, S ...
https://en.wikipedia.org/wiki/Actomyosin_ring
Sriram RamaswamySriram Ramaswamy
Kumar, Abhishek; Maitra, Ananyo; Sumit, Madhuresh; Ramaswamy, Sriram; Shivashankar, G. V. (21 January 2014). "Actomyosin ...
https://en.wikipedia.org/wiki/Sriram_Ramaswamy
Myosin binding protein C, cardiacMyosin binding protein C, cardiac
Moos C, Feng IN (Oct 1980). "Effect of C-protein on actomyosin ATPase". Biochimica et Biophysica Acta (BBA) - General Subjects ...
https://en.wikipedia.org/wiki/Myosin_binding_protein_C,_cardiac
MYH9MYH9
Moos C, Feng IN (October 1980). "Effect of C-protein on actomyosin ATPase". Biochimica et Biophysica Acta (BBA) - General ...
https://en.wikipedia.org/wiki/MYH9
Ilona BangaIlona Banga
In the process, she co-discovered actomyosin. In order to get myosin to analyze, Banga extracted it from rabbit muscles - she ... Ilona Banga (1906-1998) was a Hungarian biochemist known for co-discovering actomyosin and working to characterize how actin ... describe their groundbreaking experiments leading to the discovery of actomyosin I Studies from the Institute of Medical ...
https://en.wikipedia.org/wiki/Ilona_Banga
Transforming protein RhoATransforming protein RhoA
ROCK kinases induce actomyosin-based contractility and phosphorylate TAU and MAP2 involved in regulating myosins and other ... RhoA is prevalent in regulating cell shape, polarity and locomotion via actin polymerization, actomyosin contractility, cell ... Cytokinesis is defined by actomyosin-based contraction. RhoA-dependent diaphanous-related formins (DRFs) localize to the ... mostly actin stress fibers formation and actomyosin contractility. It acts upon several effectors. Among them, ROCK1 (Rho- ...
https://en.wikipedia.org/wiki/Transforming_protein_RhoA
Anne RidleyAnne Ridley
subscription required) Cantelli, Gaia (2016). Transcriptional programs controlling actomyosin contractility in melanoma. ethos. ...
https://en.wikipedia.org/wiki/Anne_Ridley
Sliding filament theorySliding filament theory
By the end of the 1940s Szent-Györgyi's team had postulated with evidence that contraction of actomyosin was equivalent to ... However, in one of his last contributions to muscle research, Szent-Györgyi demonstrated that actomyosin driven by ATP was the ... Cross-bridge theory states that actin and myosin form a protein complex (classically called actomyosin) by attachment of myosin ... Szent-Györgyi, A (1949). "Free-energy relations and contraction of actomyosin". The Biological Bulletin. 96 (2): 140-161. doi: ...
https://en.wikipedia.org/wiki/Sliding_filament_theory
Enzyme catalysisEnzyme catalysis
Siemankowski RF, Wiseman MO, White HD (February 1985). "ADP dissociation from actomyosin subfragment 1 is sufficiently slow to ... Lymn RW, Taylor EW (December 1971). "Mechanism of adenosine triphosphate hydrolysis by actomyosin". Biochemistry. 10 (25): 4617 ... "Kinetics of nucleoside triphosphate cleavage and phosphate release steps by associated rabbit skeletal actomyosin, measured ...
https://en.wikipedia.org/wiki/Enzyme_catalysis
Edwin W. TaylorEdwin W. Taylor
In the actomyosin ATPase cycle, the hydrolysis of ATP by enzymes leads to the generation of force and motion. Taylor also has ... The actomyosin ATP hydrolysis cycle in muscle motility was intended to show a direct correlation in the regulation of ATP ... He found the dissociation of phosphate in the actomyosin mechanism to be a slow rate-limiting step, while this step occurred ... Aware that ATP was hydrolyzed in the polymerization of actin, Taylor was confused on whether it dissociated actomyosin through ...
https://en.wikipedia.org/wiki/Edwin_W._Taylor
Keith BurridgeKeith Burridge
Korn, ED (1978). "Biochemistry of actomyosin-dependent cell motility (a review)". Proceedings of the National Academy of ...
https://en.wikipedia.org/wiki/Keith_Burridge
TroponinTroponin
TnI inhibits ATP-ase activity of acto-myosin; TnC is a Ca2+-binding subunit, playing the main role in Ca2+ dependent regulation ...
https://en.wikipedia.org/wiki/Troponin
Tropomyosin 3Tropomyosin 3
Inhibition of actomyosin MgATPase and regulation by phosphorylation". J. Biol. Chem. 265 (17): 10148-55. doi:10.1016/S0021-9258 ...
https://en.wikipedia.org/wiki/Tropomyosin_3
DurotaxisDurotaxis
The source of cytoskeletal traction is actomyosin contractility. Increased external stiffness leads to a signal transduction ... actomyosin contractility, and cytoskeletal organization. As a result, these changes can cause a cell to rearrange its ...
https://en.wikipedia.org/wiki/Durotaxis
Actin, alpha skeletal muscleActin, alpha skeletal muscle
Inhibition of actomyosin MgATPase and regulation by phosphorylation". The Journal of Biological Chemistry. 265 (17): 10148-55. ...
https://en.wikipedia.org/wiki/Actin,_alpha_skeletal_muscle
ExcimerExcimer
"Myosin cleft movement and its coupling to actomyosin dissociation". Nature Structural & Molecular Biology. 10 (10): 831-835. ...
https://en.wikipedia.org/wiki/Excimer
Focal adhesionFocal adhesion
Wolfenson, H.; Bershadsky, A.; Henis, Y. I.; Geiger, B. (2011). "Actomyosin-generated tension controls the molecular kinetics ...
https://en.wikipedia.org/wiki/Focal_adhesion
MitosisMitosis
Due to its importance in mitosis, the molecular components and dynamics of the mitotic actomyosin cortex is an area of active ... Matthews HK, Delabre U, Rohn JL, Guck J, Kunda P, Baum B (August 2012). "Changes in Ect2 localization couple actomyosin- ... Rounding forces are driven by reorganization of F-actin and myosin (actomyosin) into a contractile homogeneous cell cortex that ... Stewart MP, Helenius J, Toyoda Y, Ramanathan SP, Muller DJ, Hyman AA (January 2011). "Hydrostatic pressure and the actomyosin ...
https://en.wikipedia.org/wiki/Mitosis
Cell cortexCell cortex
The actomyosin cortex is attached to the cell membrane via membrane-anchoring proteins called ERM proteins that plays a central ... Rosa, A (2015). "Ect2/Pbl acts via Rho and polarity proteins to direct the assembly of an isotropic actomyosin cortex upon ... The cell cortex, also known as the actin cortex, cortical cytoskeleton or actomyosin cortex, is a specialized layer of ... Stewart MP, Helenius J, Toyoda Y, Ramanathan SP, Muller DJ, Hyman AA (January 2011). "Hydrostatic pressure and the actomyosin ...
https://en.wikipedia.org/wiki/Cell_cortex
Bernhard BrennerBernhard Brenner
Yu, L.C.; Brenner, B. (March 1989). "Structures of actomyosin crossbridges in relaxed and rigor muscle fibers". Biophysical ...
https://en.wikipedia.org/wiki/Bernhard_Brenner
ANLNANLN
These actomyosin rings invaginate to separate all nuclei for one another in the syncytial blastoderm. Anillin has a unique ... Goldbach P, Wong R, Beise N, Sarpal R, Trimble WS, Brill JA (May 2010). "Stabilization of the actomyosin ring enables ... Anillins are also enriched at other actomyosin rings, most significantly, those at the leading edge of the Drosophila embryo ... Anillins have also been shown to organize the actomyosin cytoskeleton into syncytial structures observed in Drosophila embryos ...
https://en.wikipedia.org/wiki/ANLN
GlysobuzoleGlysobuzole
The actomyosin filaments of β-cells contract and release insulin. Secretion of insulin in large amounts decreases the ...
https://en.wikipedia.org/wiki/Glysobuzole
ROCK1ROCK1
... is a major downstream effecter of the small GTPase RhoA and is a regulator of the actomyosin cytoskeleton which promotes ... In humans, the main function of ROCK1 is actomyosin contractility. As mentioned before, this contributes to many proximal ...
https://en.wikipedia.org/wiki/ROCK1
Cell mechanicsCell mechanics
Stewart MP, Helenius J, Toyoda Y, Ramanathan SP, Muller DJ, Hyman AA (January 2011). "Hydrostatic pressure and the actomyosin ... actomyosin assemblies (F-actin, myosin motors, and associated binding, nucleating, capping, stabilizing, and crosslinking ...
https://en.wikipedia.org/wiki/Cell_mechanics
Mitotic cell roundingMitotic cell rounding
Apart from actomyosin-related genes, several disease genes have recently been implicated in mitotic cell rounding. These ... "ETH ETH E-Collection: The Mechanism of Mitotic Rounding: Role of the Actomyosin Cortex - ETH". e-collection.library.ethz.ch. ... Sorce, B (2015). "Mitotic cells contract actomyosin cortex and generate pressure to round against or escape epithelial ... "Changes in Ect2 localization couple actomyosin-dependent cell shape changes to mitotic progression". Developmental Cell. 23 (2 ...
https://en.wikipedia.org/wiki/Mitotic_cell_rounding
Apical constrictionApical constriction
Like in Xenopus, actomyosin contractility plays a major role in constricting the apical side of the cell. The constricting ... In these cells, apical constriction occurs when actomyosin contractility folds the cell membrane to reduce the apical surface ... Lee, J.; Harland, R. M. (2007). "Actomyosin contractility and microtubules drive apical constriction in Xenopus bottle cells". ...
https://en.wikipedia.org/wiki/Apical_constriction
ADP deaminaseADP deaminase
Deutsch A, Nilsson R (1954). "On the dephosphorylation and deamination of adenosine triphosphate by actomyosin gel". Acta Chem ...
https://en.wikipedia.org/wiki/ADP_deaminase
Plakophilin-2Plakophilin-2
"Plakophilin 2 couples actomyosin remodeling to desmosomal plaque assembly via RhoA". Molecular Biology of the Cell. 21 (16): ...
https://en.wikipedia.org/wiki/Plakophilin-2
Rae Robertson-AndersonRae Robertson-Anderson
The oscillators produce actomyosin, a protein complex which contracts muscle tissue. Robertson-Anderson was made Chair of the ...
https://en.wikipedia.org/wiki/Rae_Robertson-Anderson
Manuel MoralesManuel Morales
1989) On the origin and transmission of force in actomyosin subfragment 1. Proceedings of the National Academy of Sciences USA ...
https://en.wikipedia.org/wiki/Manuel_Morales
Actin turnover ensures uniform tension distribution during cytokinetic actomyosin ring contraction | Molecular Biology of the...Actin turnover ensures uniform tension distribution during cytokinetic actomyosin ring contraction | Molecular Biology of the...
... cytokinesis is facilitated by the contraction of an actomyosin ring (AMR). The exact mechanisms that lead to this contractility ... of defective actin turnover during cytokinesis shows that robust rearrangement of actin is crucial for proper actomyosin ring ... A contractile actomyosin ring (AMR) is essential for normal cytokinesis in fungal, amoeboid, and metazoan cells, and both ... Nguyen L, Swulius MT, Aich S, Mishra M, Jensen GJ (2018). Coarse-grained simulations of actomyosin rings point to a nodeless ...
https://www.molbiolcell.org/doi/10.1091/mbc.E18-08-0511
Microtubule-Actomyosin Mechanical Cooperation during Contact Guidance Sensing<...
Microtubule-Actomyosin Mechanical Cooperation during Contact Guidance Sensing. Cell Reports. 2018 Oct 9;25(2):328-338.e6. doi: ... Microtubule-Actomyosin Mechanical Cooperation during Contact Guidance Sensing. In: Cell Reports. 2018 ; Vol. 25, No. 2. pp. 328 ... Microtubule-Actomyosin Mechanical Cooperation during Contact Guidance Sensing. Erdem D. Tabdanov, Vikram Puram, Alexander ... Microtubule-Actomyosin Mechanical Cooperation during Contact Guidance Sensing. / Tabdanov, Erdem D.; Puram, Vikram; Zhovmer, ...
https://pennstate.pure.elsevier.com/en/publications/microtubule-actomyosin-mechanical-cooperation-during-contact-guid
positive regulation of mitotic actomyosin contractile ring assembly - Ontology Browser - Rat Genome Databasepositive regulation of mitotic actomyosin contractile ring assembly - Ontology Browser - Rat Genome Database
mitotic actomyosin contractile ring assembly actin filament organization + negative regulation of mitotic actomyosin ... positive regulation of mitotic actomyosin contractile ring contraction positive regulation of mitotic actomyosin contractile ... actomyosin contractile ring assembly ; up regulation of actomyosin contractile ring assembly involved in cytokinesis after ... actomyosin contractile ring assembly involved in mitotic cytokinesis ; up regulation of mitotic actomyosin contractile ring ...
https://rgd.mcw.edu/rgdweb/ontology/view.html?acc_id=GO:1903501
The actomyosin network at the apical pole of human corneal endothelial cells | 2022 Photomicrography Competition | Nikons...The actomyosin network at the apical pole of human corneal endothelial cells | 2022 Photomicrography Competition | Nikon's...
The actomyosin network at the apical pole of human corneal endothelial cells (revealed by immunofluorescence) ...
https://www.nikonsmallworld.com/galleries/2022-photomicrography-competition/the-actomyosin-network-at-the-apical-pole-of-human-corneal-endothelial-cells
Spatiotemporal recruitment of RhoGTPase protein GRAF inhibits actomyosin ring constriction in Drosophila cellularization | eLifeSpatiotemporal recruitment of RhoGTPase protein GRAF inhibits actomyosin ring constriction in Drosophila cellularization | eLife
Actomyosin contractility is regulated by Rho-GTP in cell migration, cytokinesis and morphogenesis in embryo development. ... GRAF is enriched at the cleavage furrow tip during actomyosin assembly and initiation of ring constriction. Graf depletion ... Whereas Rho activation by Rho-GTP exchange factor (GEF), RhoGEF2 is well known in actomyosin contractility during cytokinesis ... GRAF and RhoGEF2 are present in a balance for appropriate activation of actomyosin ring constriction. RhoGEF2 depletion and ...
https://elifesciences.org/articles/63535v1
Language: English / Subject: Actomyosin / Exhibit Tags: institute - Albert Szent-Gyorgyi - Profiles in Science Search ResultsLanguage: English / Subject: Actomyosin / Exhibit Tags: institute - Albert Szent-Gyorgyi - Profiles in Science Search Results
EN (en)EN (en)
Acto-myosin cytoskeleton dependent viscosity and shear-thinning behavior of the amoeba cytoplasm. ...
https://research.pasteur.fr/en/publication/acto-myosin-cytoskeleton-dependent-viscosity-and-shear-thinning-behavior-of-the-amoeba-cytoplasm/
Casimir - BN Seminar - Stephan Grill (TU Dresden): Physics of Actomyosin-based Morphogenetic ProcessesCasimir - BN Seminar - Stephan Grill (TU Dresden): 'Physics of Actomyosin-based Morphogenetic Processes'
The actomyosin cortex is a thin layer underneath the cellular membrane that can self contract, which drives many of the large- ... BN Seminar - Stephan Grill (TU Dresden): Physics of Actomyosin-based Morphogenetic Processes. Date:. 27 January 2017 ... I will discuss physical activities of the actomyosin cytoskeleton that emerge on the mesoscale and that drive cell-scale ... We are just beginning to understand how the actomyosin cortex reshapes and deforms, and how such morphogenetic processes couple ...
https://casimir.researchschool.nl/bn-seminar-stephan-grill-tu-dresden-physics-of-actomyosin-based-morphogenetic-processes--3198.html
Actin-facilitated assembly of smooth muscle myosin induces formation of actomyosin fibrils | Journal of Cell Biology |...Actin-facilitated assembly of smooth muscle myosin induces formation of actomyosin fibrils | Journal of Cell Biology |...
To identify regulatory mechanisms potentially involved in formation of actomyosin structures in smooth muscle cells, the ... causing regeneration of actomyosin fiber bundles. The results indicate that actomyosin fibers can be spontaneously formed by F- ... Actin-facilitated assembly of smooth muscle myosin induces formation of actomyosin fibrils D Applegate, D Applegate ... Actomyosin fibers also formed after addition of F-actin to nonphosphorylated 10S myosin monomers containing the products of ATP ...
https://rupress.org/jcb/article/117/6/1223/14412/Actin-facilitated-assembly-of-smooth-muscle-myosin
Actomyosin cables by mechanical self-organization | mathtube.org
Actomyosin cables by mechanical self-organization. Speaker: Mingfeng Qiu Date: Wed, Oct 5, 2022 Location: PIMS, University of ... Supracellular actomyosin cables often drive morphogenesis in development. The origin of these cables is poorly understood. We ...
https://mathtube.org/lecture/video/actomyosin-cables-mechanical-self-organization
Browsing by Subject "Actomyosin Cytoskeleton"
Singed (human Fascin), being an parallel bundle actin cross linker, is required for cell motility in both Droso¬phila and mammalian systems, as Fascin 1-dependent actin bundles are required for the formation of filopodia ...
http://www.presiuniv.ndl.iitkgp.ac.in/xmlui/browse?type=subject&value=Actomyosin+Cytoskeleton
Regulation of actomyosin contractility | Zaidel-Bar Lab
... we have shown that activity of the small GTPase RHO-1 is essential for spermathecal contraction by activation of actomyosin, a ... as a highly tractable model of a smooth muscle-like tubular tissue to uncover novel signaling pathways controlling actomyosin ...
https://www.zaidelbarlab.com/regulation-of-actomyosin-contractil-1
Entropy production rate is maximized in non-contractile actomyosin by Daniel S. Seara, Vikrant Yadav et al."Entropy production rate is maximized in non-contractile actomyosin" by Daniel S. Seara, Vikrant Yadav et al.
To investigate this, we synthesize an actomyosin material in vitro whose active stress content can tune the network from stable ... stable state of actomyosin. Our study provides evidence that the origins of entropy production and activity-dependent ... To investigate this, we synthesize an actomyosin material in vitro whose active stress content can tune the network from stable ... stable state of actomyosin. Our study provides evidence that the origins of entropy production and activity-dependent ...
https://scholarworks.uni.edu/facpub/625/
Engineering Light-Responsive Contractile Actomyosin Systems with Genetic NanotechnologyEngineering Light-Responsive Contractile Actomyosin Systems with Genetic Nanotechnology
Due to terms of use violation, your account has been suspended by Akismet Anti-Spam Plugin. ...
https://dirstop.com/story12894043/engineering-light-responsive-contractile-actomyosin-systems-with-genetic-nanotechnology
Microtubules tune mechanosensitive cell responses | Nature MaterialsMicrotubules tune mechanosensitive cell responses | Nature Materials
Here, we show that talin- and actomyosin-dependent mechanosensing of substrate rigidity controls microtubule acetylation (a ... actomyosin contractility and traction forces. Our results reveal a fundamental crosstalk between microtubules and actin in ... Integrin-mediated focal adhesions are major sites of mechanotransduction and their connection with the actomyosin network is ... 4 Microtubule acetylation promotes acto-myosin contractility.. a, Astrocytes transfected with siCtl or siαTAT1-1, and (a. i) ...
https://www.nature.com/articles/s41563-021-01108-x?error=cookies_not_supported&code=04557934-8ef7-4888-bacc-933a5d1f5991
How Plasma Membrane and Cytoskeletal Dynamics Influence Single-Cell Wound Healing: Mechanotransduction, Tension and Tensegrity ...How Plasma Membrane and Cytoskeletal Dynamics Influence Single-Cell Wound Healing: Mechanotransduction, Tension and Tensegrity ...
Actomyosin contractile ring. Mechanical wounding; laser wounding. RhoA; Cdc42; F-actin; myosin II; microtubules. [83-89]. ... Formation of the actomyosin array is controlled by the Ca2+-dependent recruitment and activation of Rho family GTPase proteins ... Actomyosin contractile ring. As previously discussed, disruptions of the plasma membrane may be accompanied by, as well as ... Wound closure in epithelial sheets has been demonstrated to be driven by the coupling of actomyosin contraction and collective ...
https://www.intechopen.com/chapters/51269
Protein Friction and Filament Bending Facilitate Contraction of Disordered Actomyosin Networks | bioRxivProtein Friction and Filament Bending Facilitate Contraction of Disordered Actomyosin Networks | bioRxiv
I. Mendes Pinto, B. Y. Rubinstein, A. Kucharavy, J. R. Unruh, and R. Li, "Actin depolymerization drives actomyosin ring ... M. Murrell, P. W. Oakes, M. Lenz, and M. L. Gardel, "Forcing cells into shape: the mechanics of actomyosin contractility", ... M. Lenz, T. Thoresen, M. L. Gardel, and A. R. Dinner, "Contractile units in disordered actomyosin bundles arise from F-actin ... S. L. Freedman, G. M. Hocky, S. Banerjee, and A. R. Dinner, "Nonequilibrium phase diagrams for actomyosin networks", Soft ...
https://www.biorxiv.org/content/10.1101/2021.02.23.432588v1.full
Scaling behaviour in steady-state contracting actomyosin networks. - The Kennedy Institute of Rheumatology
Here, we generate contracting actomyosin networks with rapid turnover in vitro, by encapsulating cytoplasmic Xenopus egg ... Contractile actomyosin network flows are crucial for many cellular processes including cell division and motility, ... Scaling behaviour in steady-state contracting actomyosin networks. Malik-Garbi M., Ierushalmi N., Jansen S., Abu-Shah E., Goode ... Contractile actomyosin network flows are crucial for many cellular processes including cell division and motility, ...
https://www.kennedy.ox.ac.uk/publications/977246?35c4eeda-6f1a-11ed-9b08-0633602177b2
Oxygen regulates epithelial stem cell proliferation via RhoA-actomyosin-YAP/TAZ signal in mouse incisor. | Development;148(4...Oxygen regulates epithelial stem cell proliferation via RhoA-actomyosin-YAP/TAZ signal in mouse incisor. | Development;148(4...
Oxygen regulates epithelial stem cell proliferation via RhoA-actomyosin-YAP/TAZ signal in mouse incisor.. Otsu, Keishi; Ida- ... thereby promoting cortical actomyosin and stabilizing the adherens junction complex, including merlin. This leads to the ...
https://pesquisa.bvsalud.org/odontologia/resource/pt/mdl-33472844
Investigating actomyosin mediated nuclear force coupling in Hutchinson Gilford Progeria Syndrome - The University of Liverpool...Investigating actomyosin mediated nuclear force coupling in Hutchinson Gilford Progeria Syndrome - The University of Liverpool...
Waring, Thomas (2021) Investigating actomyosin mediated nuclear force coupling in Hutchinson Gilford Progeria Syndrome. PhD ...
https://livrepository.liverpool.ac.uk/3148764/
People - Biomedical Engineering - Purdue UniversityPeople - Biomedical Engineering - Purdue University
Purdues Weldon School of Biomedical Engineering strives to become the premier source of scientific discoveries and of well-educated biomedical engineers.
https://engineering.purdue.edu/BME/People/ptProfile?resource_id=89738
RTKN rhotekin [Homo sapiens (human)] - Gene - NCBIRTKN rhotekin [Homo sapiens (human)] - Gene - NCBI
involved_in actomyosin contractile ring assembly IBA Inferred from Biological aspect of Ancestor. more info ... is_active_in actomyosin contractile ring IBA Inferred from Biological aspect of Ancestor. more info ...
https://www.ncbi.nlm.nih.gov/gene/6242
Secretion of IL1 by Dedifferentiated Melanoma Cells Inhibits JAK1-STAT3-Driven Actomyosin Contractility of Lymph Node...Secretion of IL1 by Dedifferentiated Melanoma Cells Inhibits JAK1-STAT3-Driven Actomyosin Contractility of Lymph Node...
Secretion of IL1 by Dedifferentiated Melanoma Cells Inhibits JAK1-STAT3-Driven Actomyosin Contractility of Lymph Node ...
https://m.amedeo.com/35502542
Multiple- and single-molecule analysis of the actomyosin motor by nanometer-piconewton manipulation with a microneedle: Unitary...
Ishijima A, Kojima H, Higuchi H, Harada Y, Funatsu T, Yanagida T. Multiple- and single-molecule analysis of the actomyosin ... At a very low myosin-to-rod ratio, distinct actomyosin attachment, force generation (the power stroke), and detachment events ... At a very low myosin-to-rod ratio, distinct actomyosin attachment, force generation (the power stroke), and detachment events ... At a very low myosin-to-rod ratio, distinct actomyosin attachment, force generation (the power stroke), and detachment events ...
https://tohoku.pure.elsevier.com/en/publications/multiple-and-single-molecule-analysis-of-the-actomyosin-motor-by-
Podiumsdiskussion „Die Bedeutung von Drittmitteln in der ForschungsförderungPodiumsdiskussion „Die Bedeutung von Drittmitteln in der Forschungsförderung"
Herbert Schiller, PhD: The Molecular Interplay of the Actomyosin Cytoskeleton with Integrin Adhesion Sites. Friday, March 11, ...
https://www.meduniwien.ac.at/hp/cepii/veranstaltungen/single-view/?tx_ttnews%5Bpointer%5D=12&tx_ttnews%5Btt_news%5D=3441&cHash=2807c4d372e321c35728ce2606b761f5
Research - Onishi Lab | Duke BiologyResearch - Onishi Lab | Duke Biology
Is furrowing without actomyosin ring relevant to us?. We think so!!. It is clear that the contractile actomyosin ring was not ... While the actomyosin system plays an important role in improving the efficiency and fidelity of furrowing, it is not the ... How does a cell form a furrow without an actomyosin ring?. Most eukaryotic cells divide by forming a cleavage furrow. According ... We use the model green alga Chlamydomonas reinhardtii, which has no actomyosin ring yet divides by furrowing, as a model to ...
https://sites.duke.edu/onishilab/research/
Peripheral muscle endurance and the oxidative profile of the quadriceps in patients with COPD | ThoraxPeripheral muscle endurance and the oxidative profile of the quadriceps in patients with COPD | Thorax
Actomyosin ATPase II. Fibre typing by histochemical ATPase. Muscle Nerve1980;3:233-9. ...
https://thorax.bmj.com/content/59/8/673?ijkey=e66aba882a18b37275a7ce7cebfdda65f87b230a&keytype2=tf_ipsecsha
Publications | CrickPublications | Crick
Actomyosin drives cancer cell nuclear dysmorphia and threatens genome stability. Authors (11)*Tohru Takaki ...
https://www.crick.ac.uk/research/publications?f%5B0%5D=publication_type_latest_pub%3A404&f%5B1%5D=research_groups_latest_pub%3A125&f%5B2%5D=topic%3A33
Publications | Benny GeigersPublications | Benny Geiger's
Tian, B; Gabelt, BT; Geiger, B; Kaufman, PL (2009). The role of the actomyosin system in regulating trabecular fluid outflow. ... Wolfenson, H; Bershadsky, A; Henis, YI; Geiger, B (2011). Actomyosin-generated tension controls the molecular kinetics of focal ... Differential effect of actomyosin relaxation on the dynamic properties of focal adhesion proteins. PLoS ONE. 8 (9):e73549. ...
https://www.weizmann.ac.il/immunology/Geiger/publications?qt-view__publications__page=0
Wissenschaftliche Publikationen | Max-Planck-GesellschaftWissenschaftliche Publikationen | Max-Planck-Gesellschaft
Holmes, K. C.: Molecular structure of the actomyosin system in cross-striated muscle. In: Molecular Bioenergetics and ... Holmes, K. C.: Molecular structure of the actomyosin system in cross-striated muscle. In: Molecular Bioenergetics and ...
https://www.mpg.de/14599861/publikationen?seite=265
ContractilityCytoskeletonContractileContractionCytokinesisActinCorticalCleavage furrowRhoAMicrotubulesMembraneDynamicsSpatiotemporalVitroFlowsEpithelialAdhesionCrucialStiffnessNetworksEssentialRecruitmentFormDriveActivationForceSystemRoleUnderstandEnergy
Contractility8
  • Actomyosin contractility is regulated by Rho-GTP in cell migration, cytokinesis and morphogenesis in embryo development. (elifesciences.org)
  • Whereas Rho activation by Rho-GTP exchange factor (GEF), RhoGEF2 is well known in actomyosin contractility during cytokinesis at the base of invaginating membranes in Drosophila cellularization, Rho inhibition by RhoGTPase activating proteins (GAP) remains to be studied. (elifesciences.org)
  • We have found that the RhoGAP, GRAF inhibits actomyosin contractility during cellularization. (elifesciences.org)
  • Therefore, GRAF recruitment restricts Rho-GTP levels in a spatiotemporal manner for inhibiting actomyosin contractility during cellularization. (elifesciences.org)
  • We are using C. elegans spermatheca as a highly tractable model of a smooth muscle-like tubular tissue to uncover novel signaling pathways controlling actomyosin contractility. (zaidelbarlab.com)
  • Microtubule acetylation, in turn, promotes the release of the guanine nucleotide exchange factor GEF-H1 from microtubules to activate RhoA, actomyosin contractility and traction forces. (nature.com)
  • Secretion of IL1 by Dedifferentiated Melanoma Cells Inhibits JAK1-STAT3-Driven Actomyosin Contractility of Lymph Node Fibroblastic Reticular Cells. (amedeo.com)
  • For example perturbing actomyosin contractility strongly affects cell-cell adhesion formation and maturation (Angres et al. (exposed-skin-care.net)
Cytoskeleton4
  • I will discuss physical activities of the actomyosin cytoskeleton that emerge on the mesoscale and that drive cell-scale morphogenetic processes, for example the generation of active tension and active torque. (researchschool.nl)
  • More recently, we have shown that activity of the small GTPase RHO-1 is essential for spermathecal contraction by activation of actomyosin, a part of the cytoskeleton. (zaidelbarlab.com)
  • We have focused particular attention on the neurofibromatosis type 2 ( NF2 ) tumor suppressor, Merlin, and closely related ERM proteins (Ezrin, Radixin and Moesin) - membrane:cytoskeleton linking proteins that simultaneously influence membrane complexes and the cortical actomyosin cytoskeleton. (massgeneral.org)
  • We conclude that E-cadherin-based FAJs connect forming cell-cell adhesions to the contractile actomyosin cytoskeleton. (exposed-skin-care.net)
Contractile9
  • Any process that activates or increases the frequency, rate or extent of mitotic actomyosin contractile ring assembly. (mcw.edu)
  • Moreover, induction of actomyosin fibers by myosin light chain phosphorylation in the presence of actin filament networks provides a plausible hypothesis for contractile fiber assembly in situ. (rupress.org)
  • Entropy production rate is maximized in non-contractile actomyosin" by Daniel S. Seara, Vikrant Yadav et al. (uni.edu)
  • To investigate this, we synthesize an actomyosin material in vitro whose active stress content can tune the network from stable to contractile. (uni.edu)
  • We find that the balance of work and entropy does not increase monotonically and the entropy production rate is maximized in the non-contractile, stable state of actomyosin. (uni.edu)
  • Contractile actomyosin network flows are crucial for many cellular processes including cell division and motility, morphogenesis and transport. (ox.ac.uk)
  • According to the textbook model, the force for furrowing should be provided by the contractile actomyosin ring . (duke.edu)
  • We know very well that animal cells divide by centripetal ingression of the cleavage furrow , which is aided by the contractile actomyosin ring . (duke.edu)
  • It is clear that the contractile actomyosin ring was not the primary force-generating machinery for furrowing when it emerged in a cell, because the immediate progenitor of such cell could form a furrow without it. (duke.edu)
Contraction3
  • In many eukaryotes, cytokinesis is facilitated by the contraction of an actomyosin ring (AMR). (molbiolcell.org)
  • We use mathematical modelling and computation to investigate how protein friction facilitates contraction of disordered actomyosin networks. (biorxiv.org)
  • Moreover the rate of barrier establishment is delayed when actomyosin contraction is blocked or when Vinculin recruitment to the Cadherin complex is prevented. (exposed-skin-care.net)
Cytokinesis1
  • 5) Most importantly, we will use Chlamydomonas to identify novel and universal cytokinesis genes, whose roles may have been obscured in animals and fungi by the prominent actomyosin ring. (duke.edu)
Actin7
  • To identify regulatory mechanisms potentially involved in formation of actomyosin structures in smooth muscle cells, the influence of F-actin on smooth muscle myosin assembly was examined. (rupress.org)
  • Furthermore, myosin assembly caused rearrangement of actin filament networks into actomyosin fibers composed of coaligned F-actin and myosin thick filaments. (rupress.org)
  • Severin-induced fragmentation of actin in actomyosin fibers resulted in immediate disassembly of myosin thick filaments, demonstrating that actin filaments were indispensable for mediating myosin assembly in the presence of AMPPNP. (rupress.org)
  • Actomyosin fibers also formed after addition of F-actin to nonphosphorylated 10S myosin monomers containing the products of ATP hydrolysis trapped at the active site. (rupress.org)
  • However, reassembly of myosin filaments in the presence of MgATP and F-actin could be induced by phosphorylation of myosin P-light chains, causing regeneration of actomyosin fiber bundles. (rupress.org)
  • The results indicate that actomyosin fibers can be spontaneously formed by F-actin-mediated assembly of smooth muscle myosin. (rupress.org)
  • As cells expand, arrays of actomyosin are again resolved-some F-actin turnover likely occurs, but a large fraction of existing filaments rearrange. (janelia.org)
Cortical1
  • Mechanistically, we have demonstrated that, during hypoxia , HIF1α upregulation activates the RhoA signal, thereby promoting cortical actomyosin and stabilizing the adherens junction complex, including merlin . (bvsalud.org)
Cleavage furrow2
  • GRAF is enriched at the cleavage furrow tip during actomyosin assembly and initiation of ring constriction. (elifesciences.org)
  • We use the model green alga Chlamydomonas reinhardtii , which has no actomyosin ring yet divides by furrowing, as a model to understand how a cell without an actomyosin ring can form a cleavage furrow and to gain insights into the evolution of division mechanisms in a diverse range of eukaryotes from their common ancestor. (duke.edu)
RhoA1
  • Oxygen regulates epithelial stem cell proliferation via RhoA-actomyosin-YAP/TAZ signal in mouse incisor. (bvsalud.org)
Microtubules2
  • Using flat and nanotextured substrates with variable architectures and stiffness, we show that CG sensing is regulated by substrate stiffness and define a mechanical role for microtubules and actomyosin-microtubule interactions during CG sensing. (elsevier.com)
  • show that the mechanical properties of aligned extracellular matrix environments influence invasive cell behavior and define a mechanical role for microtubules and actomyosin-microtubule interactions during sensing of contact guidance cues that arise from aligned extracellular matrix. (elsevier.com)
Membrane2
  • The actomyosin cortex is a thin layer underneath the cellular membrane that can self contract, which drives many of the large-scale morphogenetic rearrangements that are observed during animal development. (researchschool.nl)
  • Actomyosin dynamics drive local membrane component organization in an in vitro active composite layer. (ncbs.res.in)
Dynamics2
  • Superresolution microscopy reveals actomyosin dynamics in medioapical arrays. (janelia.org)
  • Leading-process actomyosin coordinates organelle positioning and adhesion receptor dynamics in radially migrating cerebellar granule neurons. (stjude.org)
Spatiotemporal1
  • Thus, Cdc42 and formins play pivotal roles in heart lumen formation through the spatiotemporal regulation of the actomyosin network (Vogler, 2014). (sdbonline.org)
Vitro1
  • Here, we generate contracting actomyosin networks with rapid turnover in vitro, by encapsulating cytoplasmic Xenopus egg extracts into cell-sized 'water-in-oil' droplets. (ox.ac.uk)
Flows1
  • Symmetry breaking and emergence of directional flows in minimal actomyosin cortices. (mpg.de)
Epithelial1
  • Campos Y, Qiu X, Gomero Y, Wakefield R, Horner L, Brutkowski W, Han YG, Solecki D , Frase S, Bongiovanni A and d'Azzo A. Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier. (stjude.org)
Adhesion1
  • 2010 During junction formation it is not clear which of the different adhesion complexes forms a Meisoindigo functional link with actomyosin. (exposed-skin-care.net)
Crucial1
  • Integrin-mediated focal adhesions are major sites of mechanotransduction and their connection with the actomyosin network is crucial for mechanosensing as well as for the generation and transmission of forces onto the substrate. (nature.com)
Stiffness1
  • 2011. Actomyosin-mediated cellular tension drives increased tissue stiffness and β-catenin activation to induce epidermal hyperplasia and tumor growth. . (ucsf.edu)
Networks2
  • Fig. 3: Microtubule acetylation reorganizes the actomyosin and intermediate filament networks. (nature.com)
  • Scaling behaviour in steady-state contracting actomyosin networks. (ox.ac.uk)
Essential1
  • To understand the true evolutionary advantage of the actomyosin ring, it is essential to know what a cell without it can and cannot do. (duke.edu)
Recruitment1
  • Here, we show that talin- and actomyosin-dependent mechanosensing of substrate rigidity controls microtubule acetylation (a tubulin post-translational modification) by promoting the recruitment of α-tubulin acetyltransferase 1 (αTAT1) to focal adhesions. (nature.com)
Form1
  • How does a cell form a furrow without an actomyosin ring? (duke.edu)
Drive1
  • Supracellular actomyosin cables often drive morphogenesis in development. (mathtube.org)
Activation1
  • GRAF and RhoGEF2 are present in a balance for appropriate activation of actomyosin ring constriction. (elifesciences.org)
Force1
  • At a very low myosin-to-rod ratio, distinct actomyosin attachment, force generation (the power stroke), and detachment events were directly detected. (elsevier.com)
System2
  • While the actomyosin system plays an important role in improving the efficiency and fidelity of furrowing, it is not the primary driver. (duke.edu)
  • Molecular structure of the actomyosin system in cross-striated muscle. (mpg.de)
Role1
  • 2005 indicating that actomyosin based forces play a promoting or stabilizing role in this process. (exposed-skin-care.net)
Understand1
  • We are just beginning to understand how the actomyosin cortex reshapes and deforms, and how such morphogenetic processes couple to singnaling. (researchschool.nl)
Energy1
  • Using this method, the elementary events in energy transduction by the actomyosin motor, driven by ATP hydrolysis, were directly recorded from multiple and single molecules. (elsevier.com)
Microtubules tune mechanosensitive cell responses | Nature Materials
Microtubules tune mechanosensitive cell responses | Nature Materials (nature.com)
A composition-dependent molecular clutch between T cell signaling condensates and actin | eLife
A composition-dependent molecular clutch between T cell signaling condensates and actin | eLife (elifesciences.org)
Actin turnover ensures uniform tension distribution during cytokinetic actomyosin ring contraction | Molecular Biology of the...
Actin turnover ensures uniform tension distribution during cytokinetic actomyosin ring contraction | Molecular Biology of the... (molbiolcell.org)
People - Biomedical Engineering - Purdue University
People - Biomedical Engineering - Purdue University (engineering.purdue.edu)
Research - Onishi Lab | Duke Biology
Research - Onishi Lab | Duke Biology (sites.duke.edu)
Microtubule-Actomyosin Mechanical Cooperation during Contact Guidance Sensing<...
Microtubule-Actomyosin Mechanical Cooperation during Contact Guidance Sensing<... (pennstate.pure.elsevier.com)
Format: Text / Subject: Muscle Contraction / Genre: Articles / Periodical: The Biological Bulletin / Story Section: The...
Format: Text / Subject: Muscle Contraction / Genre: Articles / Periodical: The Biological Bulletin / Story Section: The... (profiles.nlm.nih.gov)
The actomyosin network at the apical pole of human corneal endothelial cells | 2022 Photomicrography Competition | Nikon's...
The actomyosin network at the apical pole of human corneal endothelial cells | 2022 Photomicrography Competition | Nikon's... (nikonsmallworld.com)
Publications | Crick
Publications | Crick (crick.ac.uk)
Ein unbeständiges flockenartiges Netzwerk entsteht | Max-Planck-Gesellschaft
Ein unbeständiges flockenartiges Netzwerk entsteht | Max-Planck-Gesellschaft (mpg.de)
Laboratoire Matière et Systèmes Complexes - Séminaires 2016
Laboratoire Matière et Systèmes Complexes - Séminaires 2016 (msc.univ-paris-diderot.fr)
Regulation of cellular senescence by extracellular matrix during chronic fibrotic diseases | Clinical Science | Portland Press
Regulation of cellular senescence by extracellular matrix during chronic fibrotic diseases | Clinical Science | Portland Press (portlandpress.com)
Publications | Benny Geiger's
Publications | Benny Geiger's (weizmann.ac.il)
Wissenschaftliche Publikationen | Max-Planck-Gesellschaft
Wissenschaftliche Publikationen | Max-Planck-Gesellschaft (mpg.de)
Trpm7 (transient receptor potential cation channel, subfamily M, member 7) - Rat Genome Database
Trpm7 (transient receptor potential cation channel, subfamily M, member 7) - Rat Genome Database (rgd.mcw.edu)
Begriffe mit A | Medizin-Lexikon | gesundheit.de
Begriffe mit A | Medizin-Lexikon | gesundheit.de (gesundheit.de)
Begriffe mit A | Medizin-Lexikon | gesundheit.de
Begriffe mit A | Medizin-Lexikon | gesundheit.de (gesundheit.de)
Acto-myosin cytoskeleton dependent viscosity and shear-thinning behavior of the amoeba cytoplasm - Research - Institut Pasteur
Acto-myosin cytoskeleton dependent viscosity and shear-thinning behavior of the amoeba cytoplasm - Research - Institut Pasteur (research.pasteur.fr)
William Weis' Profile | Stanford Profiles
William Weis' Profile | Stanford Profiles (profiles.stanford.edu)
Theresa grove - Valdosta State University
Theresa grove - Valdosta State University (valdosta.edu)
IJMS | Free Full-Text | The Janus Role of Adhesion in Chondrogenesis
IJMS | Free Full-Text | The Janus Role of Adhesion in Chondrogenesis (mdpi.com)
De nouveaux mécanismes impliqués dans la migration cellulaire élucidés | IRIC - Institut de recherche en immunologie et en...
De nouveaux mécanismes impliqués dans la migration cellulaire élucidés | IRIC - Institut de recherche en immunologie et en... (iric.ca)
Cell and Tissue Morphodynamics | NHLBI, NIH
Cell and Tissue Morphodynamics | NHLBI, NIH (nhlbi.nih.gov)
Cullin-5 mutants reveal collective sensing of the nucleocytoplasmic ratio in Drosophila embryogenesis: Current Biology
Cullin-5 mutants reveal collective sensing of the nucleocytoplasmic ratio in Drosophila embryogenesis: Current Biology (cell.com)
Chromatin swelling drives neutrophil extracellular trap release | Nature Communications
Chromatin swelling drives neutrophil extracellular trap release | Nature Communications (nature.com)
Profiles Collection: The Albert Szent-Gyorgyi Papers - Albert Szent-Gyorgyi - Profiles in Science Search Results
Profiles Collection: The Albert Szent-Gyorgyi Papers - Albert Szent-Gyorgyi - Profiles in Science Search Results (profiles.nlm.nih.gov)
Creator: Szent-Gyorgyi, Albert, 1893-1986 - Albert Szent-Gyorgyi - Profiles in Science Search Results
Creator: Szent-Gyorgyi, Albert, 1893-1986 - Albert Szent-Gyorgyi - Profiles in Science Search Results (profiles.nlm.nih.gov)
Publisher: Marine Biological Laboratory (Woods Hole, Mass.) - Albert Szent-Gyorgyi - Profiles in Science Search Results
Publisher: Marine Biological Laboratory (Woods Hole, Mass.) - Albert Szent-Gyorgyi - Profiles in Science Search Results (profiles.nlm.nih.gov)
Albert Szent-Gyorgyi - Profiles in Science Search Results
Albert Szent-Gyorgyi - Profiles in Science Search Results (profiles.nlm.nih.gov)
Exhibit Tags: institute - Albert Szent-Gyorgyi - Profiles in Science Search Results
Exhibit Tags: institute - Albert Szent-Gyorgyi - Profiles in Science Search Results (profiles.nlm.nih.gov)
Publisher: Marine Biological Laboratory (Woods Hole, Mass.) - Albert Szent-Gyorgyi - Profiles in Science Search Results
Publisher: Marine Biological Laboratory (Woods Hole, Mass.) - Albert Szent-Gyorgyi - Profiles in Science Search Results (profiles.nlm.nih.gov)
Exhibit Tags: institute - Albert Szent-Gyorgyi - Profiles in Science Search Results
Exhibit Tags: institute - Albert Szent-Gyorgyi - Profiles in Science Search Results (profiles.nlm.nih.gov)
Profiles Collection: The Albert Szent-Gyorgyi Papers - Albert Szent-Gyorgyi - Profiles in Science Search Results
Profiles Collection: The Albert Szent-Gyorgyi Papers - Albert Szent-Gyorgyi - Profiles in Science Search Results (profiles.nlm.nih.gov)
Albert Szent-Gyorgyi - Profiles in Science Search Results
Albert Szent-Gyorgyi - Profiles in Science Search Results (profiles.nlm.nih.gov)
Profiles Collection: The Albert Szent-Gyorgyi Papers - Albert Szent-Gyorgyi - Profiles in Science Search Results
Profiles Collection: The Albert Szent-Gyorgyi Papers - Albert Szent-Gyorgyi - Profiles in Science Search Results (profiles.nlm.nih.gov)
Publications - Microscopy and Imaging Core | NICHD - Eunice Kennedy Shriver National Institute of Child Health and Human...
Publications - Microscopy and Imaging Core | NICHD - Eunice Kennedy Shriver National Institute of Child Health and Human... (nichd.nih.gov)
Exhibit Tags: institute - Albert Szent-Gyorgyi - Profiles in Science Search Results
Exhibit Tags: institute - Albert Szent-Gyorgyi - Profiles in Science Search Results (profiles.nlm.nih.gov)