NFATC Transcription Factors: A family of transcription factors characterized by the presence of highly conserved calcineurin- and DNA-binding domains. NFAT proteins are activated in the CYTOPLASM by the calcium-dependent phosphatase CALCINEURIN. They transduce calcium signals to the nucleus where they can interact with TRANSCRIPTION FACTOR AP-1 or NF-KAPPA B and initiate GENETIC TRANSCRIPTION of GENES involved in CELL DIFFERENTIATION and development. NFAT proteins stimulate T-CELL activation through the induction of IMMEDIATE-EARLY GENES such as INTERLEUKIN-2.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Calcineurin: A CALCIUM and CALMODULIN-dependent serine/threonine protein phosphatase that is composed of the calcineurin A catalytic subunit and the calcineurin B regulatory subunit. Calcineurin has been shown to dephosphorylate a number of phosphoproteins including HISTONES; MYOSIN LIGHT CHAIN; and the regulatory subunits of CAMP-DEPENDENT PROTEIN KINASES. It is involved in the regulation of signal transduction and is the target of an important class of immunophilin-immunosuppressive drug complexes.Thioctic Acid: An octanoic acid bridged with two sulfurs so that it is sometimes also called a pentanoic acid in some naming schemes. It is biosynthesized by cleavage of LINOLEIC ACID and is a coenzyme of oxoglutarate dehydrogenase (KETOGLUTARATE DEHYDROGENASE COMPLEX). It is used in DIETARY SUPPLEMENTS.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Chromonesp38 Mitogen-Activated Protein Kinases: A mitogen-activated protein kinase subfamily that regulates a variety of cellular processes including CELL GROWTH PROCESSES; CELL DIFFERENTIATION; APOPTOSIS; and cellular responses to INFLAMMATION. The P38 MAP kinases are regulated by CYTOKINE RECEPTORS and can be activated in response to bacterial pathogens.Search Engine: Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.Databases, Genetic: Databases devoted to knowledge about specific genes and gene products.Genome, Human: The complete genetic complement contained in the DNA of a set of CHROMOSOMES in a HUMAN. The length of the human genome is about 3 billion base pairs.Activating Transcription Factor 3: An activating transcription factor that plays a key role in cellular responses to GENOTOXIC STRESS and OXIDATIVE STRESS.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Activating Transcription Factor 4: An activating transcription factor that regulates the expression of a variety of GENES involved in amino acid metabolism and transport. It also interacts with HTLV-I transactivator protein.Activating Transcription Factors: Activating transcription factors were originally identified as DNA-BINDING PROTEINS that interact with early promoters from ADENOVIRUSES. They are a family of basic leucine zipper transcription factors that bind to the consensus site TGACGTCA of the cyclic AMP response element, and are closely related to CYCLIC AMP-RESPONSIVE DNA-BINDING PROTEIN.Genes: A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.Receptors, Thyrotropin: Cell surface proteins that bind pituitary THYROTROPIN (also named thyroid stimulating hormone or TSH) and trigger intracellular changes of the target cells. TSH receptors are present in the nervous system and on target cells in the thyroid gland. Autoantibodies to TSH receptors are implicated in thyroid diseases such as GRAVES DISEASE and Hashimoto disease (THYROIDITIS, AUTOIMMUNE).Thyrotropin: A glycoprotein hormone secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Thyrotropin stimulates THYROID GLAND by increasing the iodide transport, synthesis and release of thyroid hormones (THYROXINE and TRIIODOTHYRONINE). Thyrotropin consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the pituitary glycoprotein hormones (TSH; LUTEINIZING HORMONE and FSH), but the beta subunit is unique and confers its biological specificity.Thyroid Gland: A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively.Nuclear Proteins: Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.Pulmonary Surfactant-Associated Protein A: An abundant pulmonary surfactant-associated protein that binds to a variety of lung pathogens, resulting in their opsinization. It also stimulates MACROPHAGES to undergo PHAGOCYTOSIS of microorganisms. Surfactant protein A contains a N-terminal collagen-like domain and a C-terminal lectin domain that are characteristic of members of the collectin family of proteins.Immunoglobulins, Thyroid-Stimulating: Autoantibodies that bind to the thyroid-stimulating hormone (TSH) receptor (RECEPTORS, THYROTROPIN) on thyroid epithelial cells. The autoantibodies mimic TSH causing an unregulated production of thyroid hormones characteristic of GRAVES DISEASE.Keratin-7: A type II keratin found associated with KERATIN-19 in ductal epithelia and gastrointestinal epithelia.Bibliometrics: The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)Thyroid Neoplasms: Tumors or cancer of the THYROID GLAND.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Oligonucleotide Array Sequence Analysis: Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.Pragmatic Clinical Trials as Topic: Works about randomized clinical trials that compare interventions in clinical settings and which look at a range of effectiveness outcomes and impacts.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Hypertension: Persistently high systemic arterial BLOOD PRESSURE. Based on multiple readings (BLOOD PRESSURE DETERMINATION), hypertension is currently defined as when SYSTOLIC PRESSURE is consistently greater than 140 mm Hg or when DIASTOLIC PRESSURE is consistently 90 mm Hg or more.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Gene Expression Regulation, Neoplastic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Terminology as Topic: The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.Leucine Zippers: DNA-binding motifs formed from two alpha-helixes which intertwine for about eight turns into a coiled coil and then bifurcate to form Y shaped structures. Leucines occurring in heptad repeats end up on the same sides of the helixes and are adjacent to each other in the stem of the Y (the "zipper" region). The DNA-binding residues are located in the bifurcated region of the Y.Basic-Leucine Zipper Transcription Factors: A large superfamily of transcription factors that contain a region rich in BASIC AMINO ACID residues followed by a LEUCINE ZIPPER domain.G-Box Binding Factors: A family of transcription factors found primarily in PLANTS that bind to the G-box DNA sequence CACGTG or to a consensus sequence CANNTG.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Receptors, Serotonin, 5-HT1: A subclass of G-protein coupled SEROTONIN receptors that couple preferentially to GI-GO G-PROTEINS resulting in decreased intracellular CYCLIC AMP levels.GTP-Binding Proteins: Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.Radioligand Assay: Quantitative determination of receptor (binding) proteins in body fluids or tissue using radioactively labeled binding reagents (e.g., antibodies, intracellular receptors, plasma binders).Binding, Competitive: The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Guanosine 5'-O-(3-Thiotriphosphate): Guanosine 5'-(trihydrogen diphosphate), monoanhydride with phosphorothioic acid. A stable GTP analog which enjoys a variety of physiological actions such as stimulation of guanine nucleotide-binding proteins, phosphoinositide hydrolysis, cyclic AMP accumulation, and activation of specific proto-oncogenes.Receptors, G-Protein-Coupled: The largest family of cell surface receptors involved in SIGNAL TRANSDUCTION. They share a common structure and signal through HETEROTRIMERIC G-PROTEINS.Ligands: A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)Recombinant Proteins: Proteins prepared by recombinant DNA technology.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Research: Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Baculoviridae: Family of INSECT VIRUSES containing two subfamilies: Eubaculovirinae (occluded baculoviruses) and Nudibaculovirinae (nonoccluded baculoviruses). The Eubaculovirinae, which contain polyhedron-shaped inclusion bodies, have two genera: NUCLEOPOLYHEDROVIRUS and GRANULOVIRUS. Baculovirus vectors are used for expression of foreign genes in insects.Genomics: The systematic study of the complete DNA sequences (GENOME) of organisms.
(1/213) Transcriptional repression of the cystic fibrosis transmembrane conductance regulator gene, mediated by CCAAT displacement protein/cut homolog, is associated with histone deacetylation.

Human cystic fibrosis transmembrane conductance regulator gene (CFTR) transcription is tightly regulated by nucleotide sequences upstream of the initiator sequences. Our studies of human CFTR transcription focus on identifying transcription factors bound to an inverted CCAAT consensus or "Y-box element." The human homeodomain CCAAT displacement protein/cut homolog (CDP/cut) can bind to the Y-box element through a cut repeat and homeobox. Analysis of stably transfected cell lines with wild-type and mutant human CFTR-directed reporter genes demonstrates that human histone acetyltransferase GCN5 and transcription factor ATF-1 can potentiate CFTR transcription through the Y-box element. We have found 1) that human CDP/cut acts as a repressor of CFTR transcription through the Y-box element by competing for the sites of transactivators hGCN5 and ATF-1; 2) that the ability of CDP/cut to repress activities of hGCN5 and ATF-1 activity is contingent on the amount of CDP/cut expression; 3) that histone acetylation may have a role in the regulation of gene transcription by altering the accessibility of the CFTR Y-box for sequence-specific transcription factors; 4) that trichostatin A, an inhibitor of histone deacetylase activity, activates transcription of CFTR through the Y-box element; 5) that the inhibition of histone deacetylase activity leads to an alteration of local chromatin structure requiring an intact Y-box sequence in CFTR; 6) that immunocomplexes of CDP/cut possess an associated histone deacetylase activity; 7) that the carboxyl region of CDP/cut, responsible for the transcriptional repressor function, interacts with the histone deacetylase, HDAC1. We propose that CFTR transcription may be regulated through interactions with factors directing the modification of chromatin and requires the conservation of the inverted CCAAT (Y-box) element of the CFTR promoter.  (+info)

(2/213) Bacterial peptidoglycan induces CD14-dependent activation of transcription factors CREB/ATF and AP-1.

Peptidoglycan (PGN), the major cell wall component of Gram-positive bacteria, induces secretion of cytokines in macrophages through CD14, the pattern recognition receptor that binds lipopolysaccharide and other microbial products. To begin to elucidate the mechanisms that regulate the transcription of cytokine genes, we wanted to determine which transcription factors are activated by PGN in mouse RAW264.7 and human THP-1 macrophage cells. Our results demonstrated that: (i) PGN induced phosphorylation of the transcription factors ATF-1 and CREB; (ii) ATF-1 and CREB bound DNA as a dimer and induced transcriptional activation of a CRE reporter plasmid, which was inhibited by dominant negative CREB and ATF-1; (iii) PGN induced phosphorylation of c-Jun, protein synthesis of JunB and c-Fos, and transcriptional activation of the AP-1 reporter plasmid, which was inhibited by dominant negative c-Fos; and (iv) PGN-induced activation of CREB/ATF and AP-1 was mediated through CD14. This is the first study to demonstrate activation of CREB/ATF and AP-1 transcription factors by PGN or by any other component of Gram-positive bacteria.  (+info)

(3/213) Cyclic AMP- and differentiation-dependent regulation of the proximal alphaHCG gene promoter in term villous trophoblasts.

Although the regulatory mechanisms controlling alpha and beta human chorionic gonadotrophin (HCG) expression have been investigated in choriocarcinoma cell model systems, little is known about the regulation of HCG subunit synthesis in non-tumourigenic trophoblasts. We therefore investigated alphaHCG mRNA transcription in villous cytotrophoblasts isolated from term placentae and have shown for the first time that the proximal alphaHCG gene promoter is functional in these cells. By establishing conditions which allow efficient transient transfection of immunopurified cells, we have demonstrated that a 363 bp sequence in the proximal 5' flanking region of the alphaHCG gene is sufficient to direct trophoblast-specific expression of a luciferase reporter. After 12-60 h cultivation, an increase in endogenous alphaHCG mRNA expression could be detected, indicating that aggregated villous trophoblasts undergo biochemical differentiation. Concomitantly, we observed induction of alphaHCG promoter-driven luciferase activity, suggesting that the 363 bp sequence of the proximal 5' flanking region is sufficient to direct differentiation-dependent increase of alphaHCG mRNA. Continuous luciferase expression required functional cAMP-response elements (CREs), since deletion of both recognition sequences eliminated differentiation-dependent transcription of the reporter. Elevation of cAMP values increased transcription of the wild-type construct; however, it did not affect promoter activity of the mutant plasmid. Moreover, we have demonstrated that during in-vitro differentiation, CREs interacted with increasing amounts of phosphorylated activating transcription factor/cyclic AMP response element-binding protein (ATF-1/CREB-1) suggesting that these cAMP-dependent DNA-binding factors are major determinants in regulating alphaHCG gene expression in villous trophoblasts.  (+info)

(4/213) Definition of the transcription factors which bind the differentiation responsive element of the Epstein-Barr virus BZLF1 Z promoter in human epithelial cells.

Epstein-Barr virus (EBV) is a ubiquitous human herpesvirus and an important human pathogen. Initiation of the EBV lytic cycle is dependent upon transcription of the EBV BZLF1 gene. Our previous studies of transcriptional regulation of the BZLF1 Z promoter (Zp) in human SCC12F epithelial cells identified a region within Zp that is responsive to epithelial cell differentiation. In the present study, we localize this differentiation responsive element to the CREB/AP-1-like binding site (TGACATCA) between -67 to -60 bp within Zp, previously designated ZII, and furthermore show that homodimers and heterodimers of CREB and ATF-1 specifically bind ZII. Consistent with a regulatory role for CREB and ATF-1 in differentiation dependent BZLF1 expression, ZII was able to bind approximately 3-fold more CREB and ATF-1 when incubated with nuclear extract obtained from populations of SCC12F cells enriched for the differentiated phenotype than when incubated with extract obtained from populations enriched for the undifferentiated phenotype. In addition, CREB and ATF-1 were found to increase in abundance during SCC12F differentiation. These results indicate a regulatory role for CREB and ATF-1 in differentiation-dependent expression of BZLF1 in human epithelial cells.  (+info)

(5/213) Role of the ATFa/JNK2 complex in Jun activation.

The ATFa proteins, which are members of the CREB/ATF family of transcription factors, display quite versatile properties. We have previously shown that they interact with the adenovirus E1a oncoprotein, mediating part of its transcriptional activity and heterodimerize with the Jun, Fos or related transcription factors, thereby modulating their DNA-binding specificity. In the present study, we report the sequence requirement of the N-terminal activation domain of ATFa and demonstrate the importance of specific threonine residues (Thr51 and Thr53) in addition to that of the metal-binding domain, in transcriptional activation processes. We also show that the N-terminal domain of ATFa which stably binds the Jun N-terminal kinase-2 (JNK2) (Bocco et al., 1996), is not a substrate for this kinase in vivo but, instead, serves as a JNK2-docking site for ATFa-associated partners like JunD, allowing them to be phosphorylated by the bound kinase.  (+info)

(6/213) Stress-induced stimulation of early growth response gene-1 by p38/stress-activated protein kinase 2 is mediated by a cAMP-responsive promoter element in a MAPKAP kinase 2-independent manner.

The p38/stress-activated protein kinase2 (p38/SAPK2) is activated by cellular stress and proinflammatory cytokines. Several transcription factors have been reported to be regulated by p38/SAPK2, and this kinase is involved in the control of expression of various genes. In human Jurkat T-cells, induction of the early growth response gene-1 (egr-1) by anisomycin is completely inhibited by SB203580, a specific inhibitor of p38/SAPK2a and -b. Northern blot and reporter gene experiments indicate that this block is at the level of mRNA biosynthesis. Using mutants of the egr-1 promoter, we demonstrate that a distal cAMP-responsive element (CRE; nucleotides -134 to -126) is necessary to control egr-1 induction by p38/SAPK2. Pull-down assays indicate that phospho-CRE binding protein (CREB) and phospho-activating transcription factor-1 (ATF1) bind to this element in a p38/SAPK2-dependent manner. In response to anisomycin, two known CREB kinases downstream to p38/SAPK2, MAPKAP kinase 2 (MK2) and mitogen- and stress-activated kinase 1 (MSK1), show increased activity. However, in MK2 -/- fibroblasts derived from mice carrying a disruption of the MK2 gene, the phosphorylation of CREB and ATF1 and the expression of egr-1 reach levels comparable with wild type cells. This finding excludes MK2 as an involved enzyme. We conclude that egr-1 induction by anisomycin is mediated by p38/SAPK2 and probably by MSK1. Phosphorylated CREB and ATF1 then bind to the CRE of the egr-1 promoter and cause a stress-dependent transcriptional activation of this gene.  (+info)

(7/213) Identification of a novel transcriptional regulatory element within the promoter region of the keratinocyte growth factor gene that mediates inducibility to cyclic AMP.

Keratinocyte growth factor (KGF) plays a critical role for the normal development and morphogenesis of many different tissues and organs. Furthermore, its expression is induced during wound healing and in various chronic inflammatory diseases. To determine the molecular mechanisms which regulate KGF gene induction at the transcriptional level, we carried out in vitro studies using the human KGF promoter. We have identified a novel regulatory element, TGAGGTCAG, located between -39 and -46 bp (relative to the transcription start site) in the KGF basal promoter region, which binds to inducible transcription factors as determined by electrophoretic mobility shift assay. When cloned in front of a heterologous SV40 promoter this region conferred inducibility to forskolin, a stimulator of adenylate cyclase. In contrast, various mutated forms of this region were either partially or completely impaired in their ability to mediate induction to forskolin. The TGAGGTCAG sequence shared homology to both the cAMP responsive element (CRE) and CCAAT/enhancer binding protein (C/EBP) consensus binding sites. An oligonucleotide comprising a consensus CRE binding site partially competed for the nuclear protein binding to the TGAGGTCAG site. Gel mobility supershift assays indicated that two members of the activating transcription factor (ATF) family of CRE binding proteins, ATF1 and ATF2, were part of the nuclear protein complex bound to this regulatory region. Furthermore, purified recombinant ATF2 was able to directly recognize and bind the TGAGGTCAG sequence. In contrast, no evidence was obtained for C/EBP transcription factors being part of the complex. These results suggest that members of the ATF family are involved in mediating the transcriptional regulation of the KGF gene in response to extracellular stimuli via a novel CRE regulatory element.  (+info)

(8/213) Sin1: an evolutionarily conserved component of the eukaryotic SAPK pathway.

The fission yeast Sty1/Spc1 mitogen-activated protein (MAP) kinase is a member of the eukaryotic stress-activated MAP kinase (SAPK) family. We have identified a protein, Sin1, that interacts with Sty1/Spc1 which is a member of a new evolutionarily conserved gene family. Cells lacking Sin1 display many, but not all, of the phenotypes of cells lacking the Sty1/Spc1 MAP kinase including sterility, multiple stress sensitivity and a cell-cycle delay. Sin1 is phosphorylated after stress but this is not Sty1/Spc1-dependent. Importantly, Sin1 is not required for activation of Sty1/Spc1 but is required for stress-dependent transcription via its substrate, Atf1. We find that in the absence of Sin1, Sty1/Spc1 appears to translocate to the nucleus but Atf1 is not fully phosphorylated and becomes unstable in response to environmental stress. Sin1 is also required for effective transcription via the AP-1 factor Pap1 but does not prevent its nuclear translocation. Remarkably chimaeric fusions of sin1 with chicken sin1 sequences rescue loss of sin1 function. We conclude that Sin1 is a novel component of the eukaryotic SAPK pathway.  (+info)

*  Calcineurin
... activates nuclear factor of activated T cell, cytoplasmic (NFATc), a transcription factor, by dephosphorylating it ... Calcineurin induces different transcription factors (NFATs) that are important in the transcription of IL-2 genes. IL-2 ... Shibasaki F, Kondo E, Akagi T, McKeon F (1997). "Suppression of signalling through transcription factor NF-AT by interactions ... which activates calcineurin, by binding a regulatory subunit and activating calmodulin binding. ...
*  ATF1
1993). "Activating transcription factor-1 can mediate Ca(2+)- and cAMP-inducible transcriptional activation". J. Biol. Chem. ... Sun P, Lou L, Maurer RA (1996). "Regulation of activating transcription factor-1 and the cAMP response element-binding protein ... This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper ... This gene has a pseudogene on chromosome 6. Activating transcription factor ATF1 has been shown to interact with: BRCA1, ...
*  CAMK1
1993). "Activating transcription factor-1 can mediate Ca(2+)- and cAMP-inducible transcriptional activation". J. Biol. Chem. ... McKinsey TA, Zhang CL, Olson EN (2001). "Activation of the myocyte enhancer factor-2 transcription factor by calcium/calmodulin ... Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly ... 2003). "Synapsin I is phosphorylated at Ser603 by p21-activated kinases (PAKs) in vitro and in PC12 cells stimulated with ...
*  RUVBL2
... a regulator of activating transcription factor 2 response to stress and DNA damage". Molecular and Cellular Biology. 21 (24): ... a regulator of activating transcription factor 2 response to stress and DNA damage". Molecular and Cellular Biology. 21 (24): ... "Control of nutrient-sensitive transcription programs by the unconventional prefoldin URI". Science. 302 (5648): 1208-12. doi: ... 366 (1): 179-92. doi:10.1016/j.jmb.2006.11.030. PMID 17157868. "Entrez Gene: RUVBL2 RuvB-like 2 (E. coli)". Ewing RM, Chu P, ...
*  Biliverdin reductase
... a novel regulator for induction of activating transcription factor-2 and heme oxygenase-1". The Journal of Biological Chemistry ... and mitogen-activated protein kinase (MAPK) signaling pathway. BVR acts as a means to regenerate bilirubin in a repeating redox ... by control of the upstream activator of insulin growth factor-1 (IGF-1) ... 235 (1-2): 372-81. doi:10.1111/j.1432-1033.1996.00372.x. PMID 8631357. PDB: 1GCU​; Kikuchi A, Park SY, Miyatake H, Sun D, Sato ...
*  QRICH1
"Activating Transcription Factor 7 Interacting Protein". Retrieved 23 April 2011. "AceView: Homo sapiens complex locus QRICH1, ... activating transcription factor 7-interacting protein 1. ATXN1 is involved in binding RNA in vitro and may be involved in RNA ... ATF7IP is a recruiter protein that couples transcriptional factors to the general transcription apparatus, thereby modulating ... transcription regulation and chromatin formation. QRICH1 is expressed at a high level, 3.3 times the average gene. It is ...
*  ATF7IP
Activating transcription factor 7-interacting protein 1 is a protein that in humans is encoded by the ATF7IP gene. ATF7IP has ... "Entrez Gene: ATF7IP activating transcription factor 7 interacting protein". Fujita, Naoyuki; Watanabe Sugiko; Ichimura Takaya; ... "A set of proteins interacting with transcription factor Sp1 identified in a two-hybrid screening". Mol Cell Biochem. 210 (1-2 ... 2005). "Activation of Sp1-mediated transcription by Rta of Epstein-Barr virus via an interaction with MCAF1". Nucleic Acids Res ...
*  CREB1
"Calmodulin-dependent protein kinase II potentiates transcriptional activation through activating transcription factor 1 but not ... The binding of CREB1 stimulates transcription. This protein is a CREB transcription factor that is a member of the leucine ... Don J, Stelzer G (Feb 2002). "The expanding family of CREB/CREM transcription factors that are involved with spermatogenesis". ... Pandey SC (Oct 2004). "The gene transcription factor cyclic AMP-responsive element binding protein: role in positive and ...
*  CAMP responsive element modulator
Pongubala JM, Atchison ML (Apr 1995). "Activating transcription factor 1 and cyclic AMP response element modulator can modulate ... CREM transcription factors also play an important role in many physiological systems, such as cardiac function, circadian ... This gene encodes a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular ... Don J, Stelzer G (Feb 2002). "The expanding family of CREB/CREM transcription factors that are involved with spermatogenesis". ...
*  DRG1
189 (1): 371-7. doi:10.1016/0006-291X(92)91568-B. PMID 1280421. Mahajan MA, Park ST, Sun XH (1996). "Association of a novel GTP ... 1448 (1): 109-14. doi:10.1016/s0167-4889(98)00129-3. PMID 9824680. Dunham I, Shimizu N, Roe BA, et al. (1999). "The DNA ... 1491 (1-3): 196-204. doi:10.1016/s0167-4781(00)00025-7. PMID 10760581. Strausberg RL, Feingold EA, Grouse LH, et al. (2003). " ... 189 (1): 363-70. doi:10.1016/0006-291X(92)91567-A. PMID 1449490. "Entrez Gene: DRG1 developmentally regulated GTP binding ...
*  CREB-binding protein
The CREB protein carries out its function by activating transcription, where interaction with transcription factors is managed ... "Calmodulin-dependent protein kinase II potentiates transcriptional activation through activating transcription factor 1 but not ... "BRCA1 augments transcription by the NF-kappaB transcription factor by binding to the Rel domain of the p65/RelA subunit". The ... Goto NK, Zor T, Martinez-Yamout M, Dyson HJ, Wright PE (November 2002). "Cooperativity in transcription factor binding to the ...
*  CAMK2G
"Calmodulin-dependent protein kinase II potentiates transcriptional activation through activating transcription factor 1 but not ... Wen Z, Zhong Z, Darnell JE (1995). "Maximal activation of transcription by Stat1 and Stat3 requires both tyrosine and serine ... Countaway JL, Nairn AC, Davis RJ (1992). "Mechanism of desensitization of the epidermal growth factor receptor protein-tyrosine ... and its regulation by a unique GTPase-activating protein (GAP)-like activity". J. Biol. Chem. 270 (9): 4805-12. doi:10.1074/jbc ...
*  Takafumi Uchida
"The protein level and transcription activity of activating transcription factor 1 is regulated by prolyl isomerase Pin1 in ...
*  MAP4K4
"MicroRNA-30d induces insulin transcription factor MafA and insulin production by targeting mitogen-activated protein 4 kinase 4 ... This further leads to the increase in expression and activity of specific transcription factors that respond to a variety of ... TNF-α can elevate MAP4K4 expression using transcription factors The JNK pathway is implicated in a number of physiological ... "Mitogen-activated protein kinase kinase kinase kinase 4 as a putative effector of Rap2 to activate the c-Jun N-terminal kinase ...
*  Activating transcription factor 2
... , also known as ATF2, is a protein that, in humans, is encoded by the ATF2 gene. This gene ... Activating transcription factor 2 has been shown to interact with C-jun, Casein kinase 2, alpha 1, CREB binding protein, ... "Phosphorylation of two eukaryotic transcription factors, Jun dimerization protein 2 and activation transcription factor 2, in ... Activating transcription factor GRCh38: Ensembl release 89: ENSG00000115966 - Ensembl, May 2017 GRCm38: Ensembl release 89: ...
*  C-Met
ETS1 activates MET transcription in vitro. MET transcription is activated by hypoxia-inducible factor 1 (HIF1), which is ... "Hepatocyte growth factor/scatter factor activates the ETS1 transcription factor by a RAS-RAF-MEK-ERK signaling pathway". ... MET activates the STAT3 transcription factor directly, through an SH2 domain. The beta-catenin pathway, a key component of the ... MET transcription is activated by HGF and several growth factors. MET promoter has four putative binding sites for Ets, a ...
*  CREB
... activating transcription factor-1) proteins. CREB proteins are expressed in many animals, including humans. CREB has a well- ... A Stimulus-Induced Transcription Factor Activated by A Diverse Array of Extracellular Signals". Annual Review of Biochemistry. ... The phosphorylated CREB recognizes the cAMP Response Element and serves as a transcription factor for Per1 and Per2, two genes ... CREB (cAMP response element-binding protein) is a cellular transcription factor. It binds to certain DNA sequences called cAMP ...
*  TP53
... which activates transcription factors. The N-terminus contains two complementary transcriptional activation domains, with a ... p53 also activates miR-34a and miR-145, which then repress the hESCs pluripotency factors, further instigating differentiation ... A tandem of nine-amino-acid transactivation domains (9aaTAD) was identified in the AD1 and AD2 regions of transcription factor ... Molecules of p53 with mutations in the OD dimerise with wild-type p53, and prevent them from activating transcription. ...
*  Cyclin D/Cdk4
The released E2F is then free to act as a transcription factor and it subsequently binds to DNA promoter regions and activates ... is bound with the E2F transcription factor. Once Cdk4 is activated and is bound with Cyclin D, the Cyclin D/Cdk4 complex ... Stimulation by growth factors activates any of a number of receptor tyrosine kinase (RTK) proteins. These receptor tyrosine ... SCF/Skp1 (an E3 ubiquitin ligase) helps to further inhibit p27 and thus further help activate the Cyclin D/Cdk4 complex. Also, ...
*  KLF2
... which in turn activates two pathways; the MEK5/ERK5 pathway, which activates MEF2, a transcription factor that upregulates KLF2 ... Krüppel-like factors Erythroid Krüppel-like factor Zinc finger transcription factors GRCh38: Ensembl release 89: ... It is a member of the Krüppel-like factor family of zinc finger transcription factors, and it has been implicated in a variety ... Wani MA, Wert SE, Lingrel JB (July 1999). "Lung Kruppel-like factor, a zinc finger transcription factor, is essential for ...
*  Nerve growth factor
The activated transcription factor c-Jun regulates nuclear transcription via AP-1 to increase pro-apoptotic gene transcription ... which results in the release of nuclear factor κB (NF-κB) transcription activator. NF-κB regulates nuclear gene transcription ... Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance ... Villoslada P, Genain CP (2004). "Role of nerve growth factor and other trophic factors in brain inflammation". Progress in ...
*  Tumor metabolome
... and indirectly causes other metabolic changes by activating transcription factors such as hypoxia-inducible factor 1 (HIF1A). ... This leads to inhibition of forkhead box subfamily O transcription factors, leading to the increase of glycolytic capacity. ... permitting it to activate glycolytic genes, which are normally activated by HIF-1 under hypoxic conditions. HIF1 then would ... p53 activates hexokinase 2 (HK2) that converts glucose to glucose-6-phosphate (G6P) which enters glycolysis to produce ATP, or ...
*  Protein disulfide-isomerase
... and activating transcription factor 6 (ATF6). They respond to high levels of misfolded proteins in the ER through intracellular ... 1699 (1-2): 35-44. doi:10.1016/j.bbapap.2004.02.017. PMID 15158710. Gruber CW, Cemazar M, Heras B, Martin JL, Craik DJ (Aug ... 367 (1): 43-57. doi:10.1007/s00441-016-2488-5. ISSN 0302-766X. PMC 5203823 . PMID 27632163. Oka, Ojore B. V.; Yeoh, Hui Y.; ... 43 (1): 62-70. doi:10.1016/j.freeradbiomed.2007.03.025. PMID 17561094. Hoffstrom BG, Kaplan A, Letso R, Schmid DC, Turmel RS, ...
*  AP-1 transcription factor
Navas TA, Baldwin DT, Stewart TA (November 1999). "RIP2 is a Raf1-activated mitogen-activated protein kinase kinase". J. Biol. ... Activator protein Transcription factor Hess, J; Angel, P; Schorpp-Kistner, M (1 December 2004). "AP-1 subunits: quarrel and ... June 2001). "Transcription factor decoy for activator protein-1 (AP-1) inhibits high glucose- and angiotensin II-induced type 1 ... Lee, W; Haslinger, A; Karin, M; Tjian, R (January 1987). "Activation of transcription by two factors that bind promoter and ...
*  ATF4
Activating transcription factor 4 (tax-responsive enhancer element B67), also known as ATF4, is a protein that in humans is ... "Entrez Gene: ATF4 activating transcription factor 4 (tax-responsive enhancer element B67)". Franceschi RT, Ge C, Xiao G, Roca H ... He CH, Gong P, Hu B, Stewart D, Choi ME, Choi AM, Alam J (2001). "Identification of activating transcription factor 4 (ATF4) as ... Activating transcription factor GRCh38: Ensembl release 89: ENSG00000128272 - Ensembl, May 2017 GRCm38: Ensembl release 89: ...
*  G1 phase
In order for the cell to continue through the G1-pm, there must be a high amount of growth factors and a steady rate of protein ... These complexes then activate S-Cdk complexes that move forward with DNA replication in the S phase. Concurrently, anaphase- ... the way this is phrased it is unclear whether it is transcription of the gene or translation of the mRNA gene product that is ... G1 phase and the other subphases of the cell cycle may be affected by limiting growth factors such as nutrient supply, ...
*  Casein kinase 1 isoform epsilon
The canonical Wnt Pathway involves the accumulation of β-catenin in the cytoplasm, which activates transcription factors. ... BMAL1 and CLOCK work to increase PER and CRY transcription by binding on the E-box domain upstream from the PER and CRY gene ... Once inside the nucleus, PER and CRY can work to inhibit their own transcription, while casein kinase 1 epsilon works to ... This oscillator, or "circadian clock," is made up of a transcription-translation negative feedback loop (TTNFL) in which ...
PURE liquid Topical Alpha Lipoic Acid 400x STRONGER THAN VITAMIN E & C for skin  PURE liquid Topical Alpha Lipoic Acid 400x STRONGER THAN VITAMIN E & C for skin
... such as tumor nectosis factor alpha and interleukins. This kind of deadly damage to DNA transcription, or allowing DNA to give ... This process prevents a very dangerous formation of oxidized fats called lipid peroxides, which damage DNA and activate NF-kB, ... inhibiting of the activation of transcription factor NF-kB; reduction of cellular inflammation; stimulation of AP-1, which ... Alpha Lipoic AcidComes in 1 oz. bottleUse at 3 - 5 %. ...
more infohttps://bestoutletshop.ecrater.com/p/25414829/pure-liquid-topical-alpha-lipoic
NIOSHTIC-2  Publications Search - 20035352 - Induction of metallothionein I by arsenic via metal-activated transcription factor...  NIOSHTIC-2 Publications Search - 20035352 - Induction of metallothionein I by arsenic via metal-activated transcription factor...
Metal-activated transcription factor 1 (MTF1) mediates the induction of metallothioneins I and II by zinc and stress signals. ... Metal-activated transcription factor 1 (MTF1) mediates the induction of metallothioneins I and II by zinc and stress signals. ... Induction of metallothionein I by arsenic via metal-activated transcription factor 1. Critical role of c-terminal cysteine ... The findings demonstrate a critical role of the C-terminal cysteine cluster of MTF1 in arsenic sensing and gene transcription ...
more infohttps://www.cdc.gov/niosh/nioshtic-2/20035352.html
Recombinant Human Activating Transcription Factor 1, His-tagged ATF1-1374H - Creative BioMart  Recombinant Human Activating Transcription Factor 1, His-tagged ATF1-1374H - Creative BioMart
Recombinant Human Activating Transcription Factor 1, His-tagged. Download Datasheet See All ATF1 Products. Bring this labeled ... ATF1; activatingtranscription factor 1; TREB36; EWS-ATF1;FUS/ATF-1; cyclic AMP-dependent transcription factor ATF-1; cAMP- ... ATF1 is a cyclic-AMPdependent transcription factor. ATF1 is expressed in a wide variety of celltypes and is capable of ... sequence-specific DNA binding transcription factor activity ;sequence-specific distal enhancer binding RNA polymerase II ...
more infohttps://www.creativebiomart.net/description_106497_13.htm
Activating transcription factor 7-interacting protein 1  Activating transcription factor 7-interacting protein 1
HCA RNA Cell Line for Activating transcription factor 7-interacting protein 1. ... Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription ... The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone H3 'Lys-9' ...
more infohttps://pharos.nih.gov/idg/targets/Q6VMQ6
Involvement of oxidants and AP-1 in angiotensin II-activated NFAT3 transcription factor | Cell Physiology  Involvement of oxidants and AP-1 in angiotensin II-activated NFAT3 transcription factor | Cell Physiology
Antioxidants inhibit ANG II from activating nuclear factor of activated T cells-3 (NFAT3) transcription factor in ... Activation of several transcription factors, including the nuclear factor of activated T cells-3 (NFAT3), contributes in part ... Activation of the nuclear factor of activated T cells-3 (NFAT3) transcription factor has been shown to result from endocrine ... EMSA was used to verify the activation of NFAT3 transcription factor. The binding of NFAT3 transcription factor to its cis- ...
more infohttp://ajpcell.physiology.org/content/292/4/C1248
IJMS | Free Full-Text | p21WAF1/Cip1 Regulation by hYSK1 Activates SP-1 Transcription Factor and Increases MMP-2 Expression...  IJMS | Free Full-Text | p21WAF1/Cip1 Regulation by hYSK1 Activates SP-1 Transcription Factor and Increases MMP-2 Expression...
... and diminished MMP-2 transcription and protein levels in hypoxic conditions as compared to control. Taken together, hYSK1 ... Moreover, the interaction between hYSK1 and p21WAF1/Cip1 led to the inhibition of SP-1 transcriptional activity, as revealed by ... functions by direct interaction and inhibits the p16INK4a expression and induces MMP-2 expression by its regulations of SP-1 ... a significant down-regulation of SP-1-mediated transactivation of p16INK4a promoter, and accelerated MMP-2 expression. ...
more infohttps://www.mdpi.com/1422-0067/20/2/310
NIOSHTIC-2  Publications Search - 20029921 - Chromium (VI) induces antioxidant gene HO-1 by activating the CNC BZIP...  NIOSHTIC-2 Publications Search - 20029921 - Chromium (VI) induces antioxidant gene HO-1 by activating the CNC BZIP...
Expression of a dominant negative form of Nrf2, a cap 'n' collar basic leucine zipper transcription factor, in the cells blocks ... implicating Nrf2 as the key transcription factor in the induction. Mechanistic analysis reveals that Cr (VI) increases the ... These results provide the first evidence that Cr (VI) induces antioxidant gene HO-1 important in ROS defense by activating Nrf2 ... Here, we report that Cr (VI) induces antioxidant gene HO-1 at both mRNA and protein levels in hepa1c1c7 cells. ...
more infohttps://www.cdc.gov/niosh/nioshtic-2/20029921.html
Wortmannin inhibits activation of nuclear transcription factors NF-kappaB and activated protein-1 induced by lipopolysaccharide...  Wortmannin inhibits activation of nuclear transcription factors NF-kappaB and activated protein-1 induced by lipopolysaccharide...
Whether all inflammatory agents activate nuclear transcription factors NF-kappaB and activated protein-1 (AP-1) through the ... Wortmannin inhibits activation of nuclear transcription factors NF-kappaB and activated protein-1 induced by lipopolysaccharide ... okadaic acid and tumor necrosis factor. Inhibition of NF-kappaB correlated with abrogation of the degradation of IkappaBalpha ... Manna SK1, Aggarwal BB.. Author information. 1. Cytokine Research Laboratory, Department of Bioimmunotherapy, The University of ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/10802070?dopt=Abstract
ATF1 Gene - GeneCards | ATF1 Protein | ATF1 Antibody  ATF1 Gene - GeneCards | ATF1 Protein | ATF1 Antibody
Activating Transcription Factor 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - ... ATF1 (Activating Transcription Factor 1) is a Protein Coding gene. Diseases associated with ATF1 include Melanoma Of Soft ... transcription factor activity, sequence-specific DNA binding. IEA,TAS. 2196176. GO:0003705. transcription factor activity, RNA ... Nuclear Events (kinase and transcription factor activation). Nuclear Events (kinase and transcription factor activation) ...
more infohttp://www.genecards.org/cgi-bin/carddisp.pl?gene=ATF1
THYROID TRANSCRIPTION FACTOR-1 ACTIVATES THE PROMOTER OF THE THYROTROPIN RECEPTOR GENE  - UCL Discovery  THYROID TRANSCRIPTION FACTOR-1 ACTIVATES THE PROMOTER OF THE THYROTROPIN RECEPTOR GENE - UCL Discovery
THYROID TRANSCRIPTION FACTOR-1 ACTIVATES THE PROMOTER OF THE THYROTROPIN RECEPTOR GENE. MOLECULAR ENDOCRINOLOGY , 7 (12) pp. ... THYROID TRANSCRIPTION FACTOR-1 ACTIVATES THE PROMOTER OF THE THYROTROPIN RECEPTOR GENE ... THYROID TRANSCRIPTION FACTOR-1 ACTIVATES THE PROMOTER OF THE THYROTROPIN RECEPTOR GENE. ...
more infohttp://discovery.ucl.ac.uk/1377353/
Expression of Terpenoids 1, a glandular trichome-specific transcription factor from tomato that activates the terpene synthase...  Expression of Terpenoids 1, a glandular trichome-specific transcription factor from tomato that activates the terpene synthase...
... a glandular trichome-specific transcription factor from tomato that activates the terpene synthase 5 promoter, Plant Molecular ... The transcription factor EMISSION OF BENZENOIDS II activates the MYB ODORANT1 promoter at a MYB binding site specific for ... a glandular trichome-specific transcription factor from tomato that activates the terpene synthase 5 promoter. Spyropoulou, ... a glandular trichome-specific transcription factor from tomato that activates the terpene synthase 5 promoter. Expression of ...
more infohttps://www.deepdyve.com/lp/springer_journal/expression-of-terpenoids-1-a-glandular-trichome-specific-transcription-K0BYN3a2Tp
Figure 5 from The Drosophila homolog of mammalian zinc finger factor MTF-1 activates transcription in response to heavy metals....  Figure 5 from The Drosophila homolog of mammalian zinc finger factor MTF-1 activates transcription in response to heavy metals....
Numbering refers to nucleotide position relative to transcription start. (B) Cytoplasmic RNA was isolated after metal induction ... The Drosophila homolog of mammalian zinc finger factor MTF-1 activates transcription in response to heavy metals.' ... and was transfected into S2 cells with or without a dMTF-1 expression vector. a to d indicate four MRE-like sequence motifs. ... were found by EMSA to bind relatively poorly to MTF-1 under our standard experimental conditions. (A) To evaluate their ...
more infohttps://www.semanticscholar.org/paper/The-Drosophila-homolog-of-mammalian-zinc-finger-fa-Zhang-Egli/f58a5faaa48208e69cde6553b3492a4c6d6efff4/figure/4
AID 91769 - Inhibition of nuclear factor of activated T-cells (NFAT) mediated transcription of beta-galactosidase in J.NFATZ.1...  AID 91769 - Inhibition of nuclear factor of activated T-cells (NFAT) mediated transcription of beta-galactosidase in J.NFATZ.1...
... mediated transcription of beta-galactosidase in J.NFATZ.1 cells. ... Inhibition of nuclear factor of activated T-cells (NFAT) ...
more infohttps://pubchem.ncbi.nlm.nih.gov/bioassay/91769
Human ATF1 peptide (ab22788) | Abcam  Human ATF1 peptide (ab22788) | Abcam
RNA binding protein activating transcription factor 1 fusion protein. *TREB 36. *TREB36 ... Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper Leucine Zipper ... Cyclic AMP dependent transcription factor ATF1. *Cyclic AMP-dependent transcription factor ATF-1 ...
more infohttps://www.abcam.com/human-atf1-peptide-ab22788.html
Microarray Analysis of Differential Gene Expression Profile in Peripheral Blood Cells of Patients with Human Essential...  Microarray Analysis of Differential Gene Expression Profile in Peripheral Blood Cells of Patients with Human Essential...
activating transcription factor 1. 1.563370465. 3.242605034. 107.3708967. 212159_x_at. AP2A2. adaptor-related protein complex 2 ... RABGTPase activating protein 1. -1.58675. 0.63685. 107.406. 203504_s_at. ABCA1. ATP-binding cassette, sub-family A (ABC1), ... Complement factor D (adipsin). -1.817904316. 0.378262489. 107.40645. * The student's t-statistic value; #The lowest False ... A bacterial cytotoxin identifies the RhoA exchanger factor Net1 as a key effector in the response to DNA damage. PLos One. 2008 ...
more infohttp://www.medsci.org/v08p0168.htm
GO Gene List  GO Gene List
Activating transcription factor 1. NM_007497. Gene Info. Atr. Ataxia telangiectasia and Rad3 related. NM_019864. Gene Info. ... Transcription termination factor, RNA polymerase I. NM_009442. Gene Info. Twist1. Twist homolog 1 (Drosophila). NM_011658. Gene ... Epidermal growth factor receptor. NM_207655. NM_007912. Gene Info. Egfr. Epidermal growth factor receptor. AK033431. Gene Info ... Mitogen-activated protein kinase kinase 4. NM_009157. Gene Info. Mapk15. Mitogen-activated protein kinase 15. NM_177922. Gene ...
more infohttps://cgap.nci.nih.gov/Genes/GoGeneQuery?PAGE=1&ORG=Mm&GOID=0051052
Basic leucine zipper proteins Gene Family | HUGO Gene Nomenclature Committee  Basic leucine zipper proteins Gene Family | HUGO Gene Nomenclature Committee
activating transcription factor 1. TREB36. 12q13.12. ATF2 activating transcription factor 2. CREB2. TREB7, CRE-BP1, HB16. ... basic leucine zipper ATF-like transcription factor 2. MGC20410. 11q13.1. BATF3 basic leucine zipper ATF-like transcription ... nuclear factor, erythroid 2 like 3. Nrf3. 7p15.2. NFIL3 nuclear factor, interleukin 3 regulated. IL3BP1. E4BP4, NFIL3A, NF-IL3A ... basic leucine zipper ATF-like transcription factor. B-ATF, SFA-2, BATF1. 14q24.3. ...
more infohttps://www.genenames.org/cgi-bin/genefamilies/set/506
GO Gene List  GO Gene List
Activating transcription factor 1. NM_005171. Gene Info. ATR. Ataxia telangiectasia and Rad3 related. NM_001184. Gene Info. ... E2F transcription factor 7. NM_203394. Gene Info. E2F8. E2F transcription factor 8. NM_024680. NM_001256371. NM_001256372. Gene ... Transcription termination factor, RNA polymerase I. BU940778. Gene Info. TWIST1. Twist homolog 1 (Drosophila). NM_000474. Gene ... Platelet derived growth factor C. NM_016205. Gene Info. PDGFRA. Platelet-derived growth factor receptor, alpha polypeptide. NM_ ...
more infohttps://cgap.nci.nih.gov/Genes/GoGeneQuery?PAGE=1&ORG=Hs&GOID=0051052
MSKs are required for the transcription of the nuclear orphan receptors Nur77, Nurr1 and Nor1 downstream of MAPK signalling |...  MSKs are required for the transcription of the nuclear orphan receptors Nur77, Nurr1 and Nor1 downstream of MAPK signalling |...
... activating transcription factor-1; CRE, cAMP response element; CREB, CRE-binding protein; DMEM, Dulbecco's modified Eagle's ... activating transcription factor-1)-dependent manner. Downstream of anisomycin signalling, a second ERK-dependent pathway, ... myocyte-enhancer-binding factor 2D; NGF, nerve growth factor; NGFI-B, NGF-induced B factor; NBRE, NGFI-B response element; PKA ... Stimulation of mouse embryonic fibroblasts with PMA, EGF (epidermal growth factor), TNF (tumour necrosis factor) or anisomycin ...
more infohttp://www.biochemj.org/content/390/3/749
  • This process prevents a very dangerous formation of oxidized fats called lipid peroxides, which damage DNA and activate NF-kB, a proinflammatory cursor to cytokines, such as tumor nectosis factor alpha and interleukins. (ecrater.com)
  • This kind of deadly damage to DNA transcription, or allowing DNA to give instructions to the cell, is to be avoided at all costs.Cosmetic PropertiesAlpha lipoic acid also has the ability to smooth skin by increasing its exfoliating capacity, or the removal of dead skin cells from the skin's surface. (ecrater.com)
  • In this study, we found that antioxidant enzyme catalase and antioxidants N -acetyl- l -cysteine, α-phenyl- N - tert -butylnitrone, and lipoic acid prevent ANG II from activating NFAT3 promoter-luciferase. (physiology.org)
  • A dominant negative form of NFAT3 transcription factor inhibited H 2 O 2 from activating NFAT3 promoter. (physiology.org)
  • Inactivation of AP-1 transcription factor by cotransfection of a dominant negative c-Jun, TAM67, prevented H 2 O 2 or ANG II from activating NFAT3 promoter. (physiology.org)
  • NFAT3 promoter containing the core NFAT cis -element without AP-1 binding site failed to show activation by H 2 O 2 treatment. (physiology.org)
  • Conversely, the knock-down of hYSK1 enhanced the p16 INK4a promoter activity and protein expression, and diminished MMP-2 transcription and protein levels in hypoxic conditions as compared to control. (mdpi.com)
  • The promoter of the embryonic MT gene (Mto) of Drosophila contains four MRE-like motifs (51), which, however, were found by EMSA to bind relatively poorly to MTF-1 under our standard experimental conditions. (semanticscholar.org)
  • The MSK responsive elements were mapped to two AP (activator protein)-1-like elements in the Nur77 promoter. (biochemj.org)
  • Inhibitors of DNA methyltransferase (Dnmt) and histone deacetylases (HDAC) synergistically activate the methylated metallothionein I gene ( MT-I) promoter in mouse lymphosarcoma cells. (asm.org)
  • Association of histone deacetylase 1 (HDAC1) with the promoter decreased after treatment with TSA or 5-AzaC and was abolished after treatment with both inhibitors. (asm.org)
  • In vivo genomic footprinting studies demonstrated increased occupancy of MTF-1 to metal response elements of the MT-I promoter after treatment with both inhibitors. (asm.org)
  • These results implicate involvement of multifarious factors including modified histones, MBDs, and Dnmts in silencing the methylated MT-I promoter in lymphosarcoma cells. (asm.org)
  • The synergistic activation of this promoter by these two types of inhibitors is due to demethylation of the promoter and altered association of different factors that leads to reorganization of the chromatin and the resultant increase in accessibility of the promoter to the activated transcription factor MTF-1. (asm.org)
  • An important gene associated with Melanoma of Soft Tissue is CREB1 (CAMP Responsive Element Binding Protein 1), and among its related pathways/superpathways are MAPK-Erk Pathway and Myometrial Relaxation and Contraction Pathways . (malacards.org)
  • This is reflected in notable pharmacological differences, in particular, a 100-1000-fold-lower affinity of the rat A 3 AR for certain xanthine compounds compared with the recombinant human and sheep receptors ( 1 , 5-7 ). (aspetjournals.org)
  • In the present study, we show that peptides corresponding to the MAPK-docking sites of MEK1, MEK2, Ste7, Elk-1 and MAPK phosphatase (MKP)-2 potently inhibit MEK2 phosphorylation of ERK2, ERK2 phosphorylation of Elk-1, and MKP-1 dephosphorylation of ERK2. (nih.gov)
  • Although phosphorylation of eIF2α results in global translational suppression, it specifically increases translation of activating transcription factor (ATF)4 through ribosomal leaky scanning of the mini open reading frames (ORFs) in the 5′-untranslated region (UTR) of the mRNA ( 19 , 22 , 23 ). (jimmunol.org)
  • Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. (wikipedia.org)
  • Among its related pathways are Activated TLR4 signalling and DNA damage_Role of Brca1 and Brca2 in DNA repair . (genecards.org)
  • The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3). (nih.gov)
  • Yeast-one-hybrid screens with this fragment identified a glandular trichome-specific transcription factor, designated Expression of Terpenoids 1 (SlEOT1). (deepdyve.com)
  • Expression and characterization of SUMO-conjugated metal-responsive transcription factor 1: SIM-dependent cross-interaction and distinct DNA binding activity. (semanticscholar.org)
  • A) To evaluate their potential, a minipromoter was assembled into a OVEC reporter construct as shown (for details, see Materials and Methods) and was transfected into S2 cells with or without a dMTF-1 expression vector. (semanticscholar.org)
  • They reveal that organ-specific expression of vascular endothelial growth factor is sufficient to mobilize the recruit hematopoietic cells from the bone marrow to the blood, but retention of the proangiogenic subpopulation of hematopoietic cells in peripheral organs requires an additional factor, stromal-derived factor 1 (SDF-1). (readabstracts.com)
  • Treatment of these cells with HDAC inhibitors also increased expression of the MTF-1 (metal transcription factor-1) gene as well as its DNA binding activity. (asm.org)
  • Induction of metallothionein I by arsenic via metal-activated transcription factor 1. (cdc.gov)
  • Metal-activated transcription factor 1 (MTF1) mediates the induction of metallothioneins I and II by zinc and stress signals. (cdc.gov)
  • Deletion of the C-terminal cysteine cluster or mutation of the cysteine residues abolishes or markedly reduces the transcription activation activity of MTF1 and the ability of MTF1 to restore Mt1 induction in Mtf1 knockout cells. (cdc.gov)
  • Stimulation of mouse embryonic fibroblasts with PMA, EGF (epidermal growth factor), TNF (tumour necrosis factor) or anisomycin resulted in induction of the Nur77 mRNA. (biochemj.org)
  • these include cardioprotection and neuroprotection from prolonged ischemia, bronchoconstriction, mast cell and eosinophil activation, and induction of hypotension ( 1-3 ). (aspetjournals.org)
  • ERK inhibitor PD98059 was found previously to inhibit AP-1 activation by H 2 O 2 . (physiology.org)
  • We examined the effect of the phosphatidylinositol-3-kinase (PI-3K) inhibitor wortmannin on the activation of NF-kappaB and AP-1 by different inflammatory agents. (nih.gov)
  • Wortmannin blocked NF-kappaB and AP-1 activation by lipopolysaccharide and phorbol ester but had minimal effect on activation by hydrogen peroxide, ceramide, okadaic acid and tumor necrosis factor. (nih.gov)
  • Thus, the mechanism of NF-kappaB and AP-1 activation by lipopolysaccharide and phorbol ester involves PI-3K. (nih.gov)
  • All the members of the C/EBP family, except C/EBPγ, can induce transcription, through their activation domains, by interacting with components of the basal transcription apparatus. (bionity.com)
  • 2000 May 4;473(1):113-8. (nih.gov)
  • According to recent report, as of the year 2000, 972 million people were afflicted worldwide and this figure is believed to rise to more than 1.56 billion by the year 2025 ( 1 , 2 ). (medsci.org)