A family of bacteria including numerous parasitic and pathogenic forms.

Rapid identification of Actinomycetaceae and related bacteria. (1/140)

Identification of new isolates belonging to the family Actinomycetaceae requires extensive numbers of biochemical tests, supplemented with gas-liquid chromatography determination of fermentation end products and, often, analysis of cell wall composition. This paper describes the results of the testing of 162 strains of Actinomycetaceae and related taxa for 20 different enzymatic activities including phosphatases, esterases, aminopeptidases, and glycosidases. The results of all tests were read after 4 h of incubation. The results obtained in the study provide significant new information on the biochemical properties of these groups of bacteria. An identification scheme based upon 13 selected tests, which allow the identification of these groups of bacteria within 4 h, is proposed.  (+info)

Effects of antibiotics on metabolism of peptidoglycan, protein, and lipids in Bifidobacterium bifidum subsp. pennsylvanicus. (2/140)

The formation of cell envelope components of Bifidobacterium bifidum subsp. pennsylvanicus was studied by measuring the incorporation of [(3)H]glycine, (14)C-labeled fatty acids, and N-benzoyl-[(14)C]glucosamine into the membrane protein, membrane lipids, and cell wall peptidoglycan, respectively. Inhibition of peptidoglycan synthesis by antibiotics (penicillin G, vancomycin, d-cycloserine, and bacitracin) and by the omission of glucosamine-containing growth factors caused a marked decrease in glycine incorporation into cellular as well as membrane protein, which was accompanied by a considerable enhancement of fatty acid incorporation. The uncoupling of protein and lipid synthesis led to the release of marked amounts of lipids from the cell under these conditions. Arrestment of protein synthesis by antibiotics (chloramphenicol, tetracycline, and actinomycin D) decreased peptidoglycan and lipid synthesis only partially, but did not lead to lipid release. Mg(2+) deficiency of the medium caused about 60% inhibition of growth and lipid synthesis, but protein synthesis and especially peptidoglycan synthesis were much less inhibited. Staphylococcin 1580 arrested the growth and also the synthesis of protein and peptidoglycan. However, the synthesis and turnover of lipids were considerably increased and a release of large amounts of lipids was observed. Peptidoglycan and cellular protein did not show any turnover either during normal growth or after the inhibition of cell wall and protein synthesis.  (+info)

Characterization and cloning of celR, a transcriptional regulator of cellulase genes from Thermomonospora fusca. (3/140)

CelR, a protein that regulates transcription of cellulase genes in Thermomonospora fusca (Actinomycetaceae) was purified to homogeneity. A 6-kilobase NotI-SacI fragment of T. fusca DNA containing the celR gene was cloned into Esherichia coli and sequenced. The celR gene encodes a 340-residue polypeptide that is highly homologous to members of the GalR-LacI family of bacterial transcriptional regulators. CelR specifically binds to a 14-base pair inverted repeat, which has sequence similarity to the binding sites of other family members. This site is present in regions upstream of all six cellulase genes in T. fusca. The binding of CelR to the celE promoter is inhibited specifically by low concentrations of cellobiose (0.2-0.5 mM), the major end product of cellulases. The other sugars tested did not affect binding at equivalent or 50-fold higher concentrations. The results suggest that CelR may act as a repressor, and that the mechanism of induction involves a direct interaction of CelR with cellobiose.  (+info)

Denitrobacterium detoxificans gen. nov., sp. nov., a ruminal bacterium that respires on nitrocompounds. (4/140)

A new group of anaerobic, Gram-positive, high G + C (56-60 mol%) bacteria was isolated from the bovine rumen. Of four strains characterized, all were non-motile and none produced spores. The isolates did not produce indole or H2S and did not hydrolyse gelatin. Cells of each strain exhibited similar rod-shaped morphology (0.5-1.0 x 1.0-1.5 microns) although bulbous ends were sometimes present. None of the four strains were able to grow via oxidation of a variety of potentially fermentable substrates but rather obtained energy for growth via anaerobic respiration processes, oxidizing hydrogen, formate or lactate for reduction of various oxidized nitrogen compounds. Trimethylamine oxide and DMSO were also used as electron acceptor. All four strains shared greater than 99% 16S rRNA gene sequence identity. The closest match found between the 16S rRNA gene sequence of all four strains, NPOH1T, NPOH2, NPOH3 and MAJ1, to sequences available in GenBank was that of Coriobacterium glomerans (86% sequence similarity), a phenotypically dissimilar anaerobe within the class Actinobacteria. To accommodate these bacteria the creation of a new genus and species, Denitrobacterium detoxificans, for placement within the family Coriobacteriaceae is proposed. The type strain, NPOH1T (ATCC 700546T), grew equally well over a narrow range of incubation temperatures tested (32-39 degrees C).  (+info)

A novel actinomycete from sugar-cane bagasse: Saccharopolyspora hirsuta gen. et. sp. nov. (5/140)

A new species of nocardioform actinomycete isolated from spontaneously heated sugar-cane bagasse is described as Saccharopolyspora hirsuta gen. et sp. nov. It has affinities with species of both Nocardia and Actinomadura but can be distinguished from both genera by its morphology, sporulation, wall and lipid analyses, antibiotic resistance, degradation and carbon utilzation tests.  (+info)

Arcanobacterium pluranimalium sp. nov., isolated from porpoise and deer. (6/140)

Two strains of a previously undescribed Arcanobacterium-like bacterium were isolated from a dead harbour porpoise and a dead sallow deer. Biochemical testing and PAGE analysis of whole-cell proteins indicated that the strains were phenotypically closely related to each other and distinct from previously described Actinomyces and Arcanobacterium species. Comparative 16S rRNA gene sequencing studies showed the bacterium to be a hitherto unknown subline within the genus Arcanobacterium. Based on phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium be classified as Arcanobacterium pluranimalium sp. nov. The type strain of Arcanobacterium pluranimalium is CCUG 42575T (= CIP 106442T).  (+info)

Cloning, expression, and characterization of a neuraminidase gene from Arcanobacterium pyogenes. (7/140)

Arcanobacterium pyogenes is an opportunistic pathogen, associated with suppurative infections in domestic animals. In addition to pyolysin, a pore-forming, cholesterol-binding toxin, A. pyogenes expresses a number of putative virulence factors, including several proteases and neuraminidase activity. A 3,009-bp gene, nanH, was cloned and sequenced and conferred neuraminidase activity on an Escherichia coli host strain. The predicted 107-kDa NanH protein displayed similarity to a number of bacterial neuraminidases and contained the RIP/RLP motif and five copies of the Asp box motif found in all bacterial neuraminidases. Recombinant His-tagged NanH was found to have pH and temperature optima of 5.5 to 6.0 and 55 degrees C, respectively. Insertional deletion of the nanH gene resulted in the reduction, but not absence, of neuraminidase activity, indicating the presence of a second neuraminidase gene in A. pyogenes. NanH was localized to the A. pyogenes cell wall. A. pyogenes adhered to HeLa, CHO, and MDBK cells in a washing-resistant manner. However, the nanH mutant was not defective for adherence to epithelial cells. The role of NanH in host epithelial cell adherence may be masked by the presence of a second neuraminidase in A. pyogenes.  (+info)

Structure of the major glycolipid from Rothia dentocariosa. (8/140)

Structural studies of the major glycolipid isolated from Rothia dentocariosa were carried out by specific chemical degradation and nuclear magnetic resonance spectroscopy. The glycolipid was found to be a dimannosylacylmonoglyceride in which the carbohydrate part was the glycerol-linked dimannoside alpha-D-Manp-(1-->3)-alpha-D-Manp-(1-->3)-sn-Gro, and the internal mannose was esterified at C-6 by fatty acid residue. The other fatty acyl chain substituted the primary methylene position of glycerol. The occurrence of this glycolipid is limited to the related microorganisms. The structural characteristics can facilitate the differentiation of some genera.  (+info)

Arcanobacterium pyogenes Pyolysin: member of thiol-activated cytolysin family; isolated from Arcanobacterium pyogenes; amino acid sequence in first source; GenBank U84782
SUMMARY Rothia gen. nov. (Actinomycetaceae) is proposed as the name of a monotypic genus with the species Rothia dentocariosus comb. nov. (basionym Actinomyces dentocariosus Onisi 1949), synonyms Nocardia dentocariosus (Onisi) Roth 1957; Nocardia salivae Davis and Freer 1960, No strain of Onisi's original isolates is extant. ATCC 17931 is described and proposed as the neotype strain of Rothia dentocariosus (Onisi) Georg and Brown 1967.
Looking for Bacterionema? Find out information about Bacterionema. A genus of bacteria in the family Actinomycetaceae; characteristically cells are rods with filaments attached and produce filamentous microcolonies;... Explanation of Bacterionema
Trypsin-like activity (TLA), clinical parameters and TLA-postitive bacteria were examined in periodontitis and healthy sites in dogs. TLA was markedly higher in periodontitis than at healthy sites. There was good correlation between TLA positivity and severity of periodontal disease. The proportions of TLA-positive bacteria to total isolates in periodontitis and healthy sites were 21.1% and 2.1%, respectively. Among TLA-positive bacteria in periodontitis sites, 4.4% showed strong TLA activity, 35.3% showed moderate and 60.3% showed weak activity. In the healthy sites, all the TLA-positive bacteria showed weak activity. In all, 90% of the total number of TLA-positve bacteria were identified as belonging to the family Actinomycetaceae; 40% of bacteria belonging to the family Actinomycetaceae were identified as genus Corynebacterium with moderate trypsin-like activity and the remaining 60% were identified as genus Actinomyces with weak activity. Obligately anaerobic bacteria accounted for only 5.9% of the
As actinomicetáceas (Actinomycetaceae) son unha familia de actinobacterias que contén o xénero medicamente importante Actinomyces. As Actinomycetaceae comprenden tres xéneros potencialmente patóxenos: Actinomyces, Nocardia, e Streptomyces. Estes organismos están estreitamente relacionados coas micobacterias pero inicialmente foran clasificadas como fungos (actinomicetos) e críase que eran unhas formas de transición entre as bacterias e os fungos. ...
As base substitutions within domain V of the 23S rRNA can result in ML resistance (17), sequencing of this gene region in these A. pyogenes isolates was undertaken. Primers 23S-1 (5′-AGTTCCGACCTGCACGAATGGC-3′) and 23S-2 (5′-GTTCGTCCGTCCCGGTCCTCTC-3′) were used to amplify a product of 728 bp, equivalent to bases 1953 to 2680 of the E. coli 23S rRNA gene (GenBank accession no. U70214 ), from the four macrolide-resistant and three macrolide-susceptible A. pyogenes isolates. The sequences of the PCR products were determined by using automated DNA sequencing.. Mutations were identified by aligning the sequences using CLUSTAL W (13), with the sequence of the MLsA. pyogenes isolate BBR1 being designated the wild type. Of the other MLs isolates, OX-5 has a wild-type sequence and OX-9 has a G2137T substitution (E. coli numbering). As OX-9 is MLs, it is unlikely that the G2137T change contributes to ML resistance, and it probably represents a naturally occurring polymorphism in the 23S rRNA ...
ID K0ZJ08_9ACTO Unreviewed; 534 AA. AC K0ZJ08; DT 28-NOV-2012, integrated into UniProtKB/TrEMBL. DT 28-NOV-2012, sequence version 1. DT 25-OCT-2017, entry version 38. DE RecName: Full=Chromosomal replication initiator protein DnaA {ECO:0000256,HAMAP-Rule:MF_00377, ECO:0000256,RuleBase:RU000577}; GN Name=dnaA {ECO:0000256,HAMAP-Rule:MF_00377}; GN ORFNames=HMPREF9241_00388 {ECO:0000313,EMBL:EJZ87760.1}; OS Actinomyces turicensis ACS-279-V-Col4. OC Bacteria; Actinobacteria; Actinomycetales; Actinomycetaceae; OC Actinomyces. OX NCBI_TaxID=883077 {ECO:0000313,EMBL:EJZ87760.1, ECO:0000313,Proteomes:UP000003994}; RN [1] {ECO:0000313,EMBL:EJZ87760.1, ECO:0000313,Proteomes:UP000003994} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ACS-279-V-Col4 {ECO:0000313,EMBL:EJZ87760.1, RC ECO:0000313,Proteomes:UP000003994}; RG The Broad Institute Genome Sequencing Platform; RA Earl A., Ward D., Feldgarden M., Gevers D., Saerens B., RA Vaneechoutte M., Walker B., Young S.K., Zeng Q., Gargeya S., RA ...
ID A0A0X8K3S2_9ACTO Unreviewed; 207 AA. AC A0A0X8K3S2; DT 13-APR-2016, integrated into UniProtKB/TrEMBL. DT 13-APR-2016, sequence version 1. DT 25-OCT-2017, entry version 10. DE RecName: Full=Superoxide dismutase {ECO:0000256,RuleBase:RU000414}; DE EC=1.15.1.1 {ECO:0000256,RuleBase:RU000414}; GN ORFNames=AXE84_11840 {ECO:0000313,EMBL:AME00013.1}; OS Actinomyces oris. OC Bacteria; Actinobacteria; Actinomycetales; Actinomycetaceae; OC Actinomyces. OX NCBI_TaxID=544580 {ECO:0000313,EMBL:AME00013.1, ECO:0000313,Proteomes:UP000067270}; RN [1] {ECO:0000313,EMBL:AME00013.1, ECO:0000313,Proteomes:UP000067270} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=T14V {ECO:0000313,EMBL:AME00013.1, RC ECO:0000313,Proteomes:UP000067270}; RA Wen L., He K., Yang H.; RL Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Destroys radicals which are normally produced within the CC cells and which are toxic to biological systems. CC {ECO:0000256,RuleBase:RU000414}. CC -!- CATALYTIC ...
Lineage: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinobacteria; Actinomycetales; Actinomycetaceae; ...
Lineage: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; ...
Bovi-Sera Serum Antibodies from Colorado - For use as an aid in the prevention and treatment of enteric and respiratory conditions in cattle and sheep caused by Arcanobacterium pyogenes, E. coli, Mannheimia haemolytica, Pasteurella multocida and Salmonella typhimurium. Administer IM or subcut.
57) Abstract:. The invention relates to veterinary Microbiology and can be used in bacteriological laboratories for the preparation of diagnostic kits. The strain obtained using traditional microbiological techniques from the urine of pigs with clinical signs of urocystic and pyelonephritis. The strain is deposited in the Institute of culture collections of microorganisms GMCVB Vector number 0699. Expressed antigenic properties and the ability to quickly and easily multiply on artificial and natural environments make use of this strain as a reference strain in the preparation of diagnostic kits. The invention relates to the field of veterinary Microbiology, epizootiology and can be used in bacteriological laboratories.. In the claimed invention was set the task of finding a strain of bacteria, easy and fast breeding on natural and artificial nutrient media and has marked antigenic properties.. Known strains of Actinobaculum (Corynebacterium, Eubacterium, Actinomyces) suis allocated M. A. Soltys, ...
Corynebacterium (/kɔːrˈaɪnəbækˌtɪəriəm/, /kɔːrˈɪnə-/) is a genus of bacteria that are gram-positive and aerobic. They are bacilli (rod-shaped), and in some phases of life they are, more particularly, club-shaped, which inspired the genus name (coryneform means club-shaped). They are widely distributed in nature in the microbiota of animals (including the human microbiota) and are mostly innocuous. Some are useful in industrial settings such as C. glutamicum. Others can cause human disease, including most notably diphtheria, which is caused by C. diphtheriae. As with various species of a microbiota (including their cousins in the genera Arcanobacterium and Trueperella), they usually are not pathogenic but can occasionally opportunistically capitalize on atypical access to tissues (via wounds) or weakened host defenses. The genus Corynebacterium was created by Lehmann and Neumann in 1896 as a taxonomic group to contain the bacterial rods responsible for causing diphtheria. The ...
Immunological adjuvants obtainable from already-known water-soluble adjuvants containing peptidoglycane fragments, which include saccharide units formed from N-acetylglucosamine and from an N-acyl-muramic acid wherein the acyl group is a glycolyl or acetyl group and to the muramyl group there are attached peptide chains, by modification of said already-known water-soluble adjuvants so that they bear acylating groups derived from physiologically-acceptable carboxylic polyacids, especially carboxylic diacids, and wherein preferably the acylating groups themselves include free carboxylic acid groups, for instance being derived from phthalic acid or succinic acid.
The invention relates to a process for the preparation of fast-dissolving pharmaceutical preparations from difficultly soluble active substances, wherein an aqueous suspension is made from the active substance and one or more water-soluble adjuvants and then the resulting aqueous suspension is processed, with removal of the water, by methods conventional per se, to form solid pharmaceutical preparations. The invention also relates to fast-dissolving pharmaceutical preparations of active substances having a dissolution rate of at least 70% after 30 minutes, prepared in accordance with the process of the invention.
Arcanobacterium haemolyticum ATCC ® BAA-1784™ Designation: Vitek 1656 TypeStrain=False Application: Quality control strain
Arcanobacterium haemolyticum ATCC ® BAA-1784™ Designation: Vitek 1656 TypeStrain=False Application: Quality control strain
en] The activity of the D-alanyl-D carboxypeptidase from the penicillin-resistant Streptomyces albus G is not or very little affected by penicillins and related antibiotics. The molecular basis for the mechanism of action of penicillin is discussed. The Streptomyces albus G D-alanyl-D carboxypeptidase appears as a model for the study of a mechanism of penicillin resistance that does not involve the enzymatic degradation of the antibiotic. Vancomycin and ristocetin are shown to inhibit the hydrolysis of sensitive peptides by the Streptomyces albus G D-alanyl-D carboxypeptidase and the mechanism of inhibition is discussed ...
The reactions of beta-lactamases of Actinomadura R39 and Streptomyces albus G with clavulanate proceed along branched pathways. Both enzymes perform the hydrolysis of this beta-lactam with rather high efficiencies (kcat. = 18s-1 and 52s-1 respectively). If large clavulanate/enzyme ratios are used, complete inactivation of the enzymes is observed. At lower ratios, inactivation is only partial. Irreversible inactivation occurs after 400 and 20000 turnovers for the A. R39 and S. albus G enzymes respectively. With the A. R39 beta-lactamase, a transiently inhibited complex is also formed that remains undetectable with the S. albus G beta-lactamase. Kinetic models are presented and studied for the interaction between clavulanate and both enzymes. A tentative general reaction scheme is also discussed.. ...
Corynebacterium matruchotii is a species of bacteria in the genus Corynebacterium. Corynebacteria occur within the normal flora of the human body. Corynebacterium matruchotii are Gram positive bacilli with long filaments and short, thick terminal ends. C. matruchotii is a bacterium of significance within the oral cavity and comprises the central filament of corn-cob formations (formations in which Streptococcus sanguinis bacteria bind to and surround C. matruchotii to create a corn-cob appearance). C. matruchotii can be isolated from dental plaque, although is it not known to be associated with the etiology of dental diseases. Corynebacterium. List of Prokaryotic Names with Standing in Nomenclature. Retrieved 22 February 2017. Paster, B. J.; Boches, S. K.; Galvin, J. L.; Ericson, R. E.; Lau, C. N.; Levanos, V. A.; Sahasrabudhe, A.; Dewhirst, F. E. (15 June 2001). Bacterial Diversity in Human Subgingival Plaque. Journal of Bacteriology. 183 (12): 3770-3783. ...
Numerous studies relate differences in microbial communities to human health and disease; however, little is known about microbial changes that occur postmortem or the possible applications of microbiome analysis in the field of forensic science. The aim of this review was to study the microbiome and its applications in forensic sciences and to determine the main lines of investigation that are emerging, as well as its possible contributions to the forensic field. A systematic review of the human microbiome in relation to forensic science was carried out by following PRISMA guidelines. This study sheds light on the role of microbiome research in the postmortem interval during the process of decomposition, identifying death caused by drowning or sudden death, locating the geographical location of death, establishing a connection between the human microbiome and personal items, sexual contact, and the identification of individuals. Actinomycetaceae, Bacteroidaceae, Alcaligenaceae, and Bacilli play ...
The urinary microbiota was shown to contain: a total of 217 bacterial isolates from 77 different genera were isolated from OAB patients, while 66 bacterial isolates from 33 different genera were isolated from control patients. Organisms isolated solely from OAB patient urines included Actinobaculum schaalii, Aerococcus urinae, Arthrobacter cumminsii, and Oligella urethralis; each has been reported to cause UTI ...
1) De Meester F, et al. (1987) The active sites of the beta-lactamases of Streptomyces cacaoi and Streptomyces albus G.. Biochem J 244(2):427-32 PubMed: 2822004 ...
1) Dehottay P, et al. (1987) Nucleotide sequence of the gene encoding the Streptomyces albus G beta-lactamase precursor.. Eur J Biochem 166(2):345-50 PubMed: 3038538 ...
Collins, C. L. ; Dunshea, F. R. ; Henman, D. J. ; McCauley, I. ; King, R. H., 2005. The apparent ileal digestibility of amino acids in common vetch (Vicia sativa cv. Morava). Aust. J. Exp. Agric., 45 (6): 705- ...
FX Reconciliations Accountant where your main responsibility will be to reconcile the companies foreign exchange transactions. You will also be responsible for the positing of journal entries, and general maintenance of the ledgers.
Introduction. Trueperella pyogenes (previously, Corynebacterium pyogenes) is an opportunistic pathogen, causing suppurative infections in a wide range of hosts, including avians (Barbour et al., 1991) and domestic animals (Addo et al., 1977). It is known to spread hematogenously to cause abscesses and suppurative lesions in various organs and tissues (Tolle et al., 1983). This organism has been reported to cause liver abscesses in large animals like cattle (Narayananet al., 1998), but its role as an etiological agent for hepatic abscesses in birds like pigeons is yet to be established. The present study reports the isolation and characterization of Trueperella pyogenes from a hepatic abscess in an american fan tailed pigeon.. Materials and Methods. A commercial flock of American-fan tailed pigeons from Thrissur, Kerala was presented with heavy morbidity and mortality. A detailed post-mortem examination of a representative bird, revealed air-sacculitis accompanied by hepatitis and abscesses on ...
Tindall 2019 Tindall BJ. Arachnia propionica (Buchanan and Pine 1962) Pine and Georg 1969 (Approved Lists 1980), Propionibacterium propionicum corrig. (Buchanan and Pine 1962) Charfreitag et al. 1988 and Pseudopropionibacterium propionicum (Buchanan and Pine 1962) Scholz and Kilian 2016 and the nomenclatural consequences of changes in the taxonomy of the genus Propionibacterium. Int J Syst Evol Microbiol 2019; 69:2612-2615. ...
Animal health depends on the ability of immune cells to kill invading pathogens, and on the resilience of tissues to tolerate the presence of pathogens. Trueperella pyogenes causes tissue pathology in many mammals by secreting a cholesterol-dependent cytolysin, pyolysin (PLO), which targets stromal cells. Cellular cholesterol is derived from squalene, which is synthesized via the mevalonate pathway enzymes, including HMGCR, FDPS and FDFT1. The present study tested the hypothesis that inhibiting enzymes in the mevalonate pathway to reduce cellular cholesterol increases the resilience of stromal cells to PLO. We first verified that depleting cellular cholesterol with methyl-β-cyclodextrin increased the resilience of stromal cells to PLO. We then used siRNA to deplete mevalonate pathway enzyme gene expression, and used pharmaceutical inhibitors, atorvastatin, alendronate or zaragozic acid to inhibit the activity of HMGCR, FDPS and FDFT1, respectively. These approaches successfully reduced cellular ...
Granuloma containing necrotic debris in the center and surrounded by macrophages, giant cells, and epithelioid cells in the periphery (H&E stain, X200). Bacteriological methods and Results. Abscess contents from different areas of the lungs were aseptically collected and plated on blood agar and MacConkey media, and incubated aerobically at 37°C. Culture plates were examined daily for 3 days. The growth was classified as heavy, moderate, and light. Three or less colonies were considered insignificant, and probably contaminants. The bacteria were identified based on colony morphology, Gram stain, classic biochemical tests, CAMP test (Quinn et al., 1994, Funke and Bernard, 2011), and by using a commercial bacterial identification system: API Coryne (bioMerieux Inc., Durham, NC, USA). Antimicrobial susceptibility tests were done against 12 drugs using the disk diffusion method as recommended by the Clinical and Laboratory Standards Institute (CLSI), and the zone sizes were interpreted as per ...
Wellcome Trust Sanger Institute, Genome Research Limited (reg no. 2742969) is a charity registered in England with number 1021457 , Legal , Cookie Policy , Data Sharing Unless otherwise stated the content of this website is copyright Genome Research Limited, licenced under the Creative Commons Attribution 3.0 Unported License. URI: ; retrieved at 03.29 am, 13th December 2017. ...
This chapter deals with aerobically growing, asporogenous, irregularly shaped, non-partially acid-fast, Gram-positive rods generally called coryneforms. Genera mainly covered include Corynebacterium, Turicella, Arthrobacter, Brevibacterium, Dermabacter, Helcobacillus, Rothia, Exiguobacterium, Oerskovia, Cellulomonas, Cellulosimicrobium, Microbacterium, Curtobacterium, Leifsonia, Pseudoclavibacter, Auritidibacter, Arcanobacterium, Trueperella, and Gardnerella. Information is presented on epidemiology and transmission, clinical significance, direct examination, isolation procedures, and identification of coryneforms. Taxa are described in detail with emphasis on identification tools for the clinical microbiologist. Finally, antimicrobial susceptibility testing procedures and resistance patterns of coryneforms are outlined.
Strains from subclinical mastitis, from the genital tract and tonsils of cattle, from tonsils of a goat and a cat and from the crop and the respiratory tract of canaries were found to constitute a new streptococcal species, for which the name Streptococcus pluranimalium sp. nov. is proposed. Sequencing of 16S rRNA showed that Streptococcus thoraltensis and Streptococcus hyovaginalis were its closest known phylogenetic relatives. The new species showed some phenotypic resemblance to the poorly described species Streptococcus acidominimus, but whole-cell protein analysis and 16S rRNA sequencing revealed that the new species was only distantly related to the type strain of S ...
The licences you must have and the transport conditions you must follow for bringing non-specified animal pathogens or carriers into the UK.
five structurally related heptadecapeptides rich in hydrophobic amino acids have been discovered in the venom of the bumblebee megabombus pennsylvanicus. we have named them bombolitin i (ile-lys-ile-thr-thr-met-leu-ala-lys-leu-gly-lys-val-leu-ala-his-val-nh2 ), bombolitin ii (ser-lys-ile-thr-asp-ile-leu-ala-lys-leu-gly-lys-val-leu-ala-his-val-nh2 ), bombolitin iii (ile-lys-ile-met-asp-ile-leu-ala-lys-leu-gly-lys-val-leu-ala-his-val-nh2 ), bombolitin iv (ile-asn-ile-lys-asp-ile-leu-ala-lys-leu-va ...
If you meant EQUAL? compares the first areas and returns TRUE if they have the same content, otherwise it compares the content in the second areas. then we agree. In other words, its an IF/ELSE rather than ALL set of comparisons. Agreed? -jn- ___ ___ ___ \/ 2 + \/ 2 = 4 (for sufficiently large approximations of \/ 2 ) joeldotneelyatfedexdotcom ...
Complete article is available online.. PDF version is available online.. Trueperella abortisuis is a gram-positive bacterium that has been previously identified in aborted porcine feti and placentae located in Asia and Europe. Routine microbiological screening of extended boar semen from a US mid-Atlantic stud identified delayed growth of very small white colonies on both brain-heart infusion and sheep-blood agars after 48 to 72 hours incubation at 37°C under aerobic conditions. Isolate identification was performed using matrix-assisted laser desorption-ionization time of flight (MALDI-TOF) mass spectrometry, with T abortisuis identified (MALDI biotyper score = 2.103). After storage at 16°C and 72 hours post collection, total and progressive motility parameters had decreased in extended semen samples positive for T abortisuis. Further work is needed to elucidate the role T abortisuis may play in extended boar semen quality, extended semen longevity, and sow reproductive performance.. Keywords: ...
The microflora of 55 patients with leprosy skin ulcers was studied and related to a weighted inflammatory score (IS). The control group consisted of 18 ulcers with different underlying pathology. Leprosy ulcers were characterized by the exclusive presence of two types of branching gram-positive rods; a particular interesting proposal is that Mycobacterium leprae share common antigens with these unusual leprosy ulcer associated organisms and group G beta-hemolytic streptococci. In the leprosy group, corynebacteria and branching rods accounted for 97% of gram-positive bacilli and Bacillus species constituted only 3%. In the control group, B. species formed 50% of gram-positive rods; the rest were corynebacteria (p = 0.03). In the leprosy group, one third of the gram-positive bacteria were branching rods; none of them was acid fast. Ten of them were identified as Arcanobacterium haemolyticum, and the remaining 7 could not be identified. The IS of leprosy patients was lower than in the control group. The
TY - CHAP. T1 - Diseases caused by actinomyces species. AU - Booth, James. PY - 2007. Y1 - 2007. N2 - The genus Actinomyces consists of facultatively anaerobic and obligately anaerobic gram-positive filamentous rods that microscopically resemble the hyphae of the eukaryotic fungi. Actinomyces are true bacteria (prokaryotic). They should not be confused with the aerobic actinomycetes, which includes Nocardia and related bacteria that they resemble microscopically. The most common infection due to Actinomyces is actinomycosis, although this term can be applied to similar infections caused by other species, such as Propionibacterium propionicum.. AB - The genus Actinomyces consists of facultatively anaerobic and obligately anaerobic gram-positive filamentous rods that microscopically resemble the hyphae of the eukaryotic fungi. Actinomyces are true bacteria (prokaryotic). They should not be confused with the aerobic actinomycetes, which includes Nocardia and related bacteria that they resemble ...
IRMNG (2018). Lactobacillus rhamnosus (Hansen 1968) Collins et al., 1989. Accessed at: http://www.irmng.org/aphia.php?p=taxdetails&id=10032442 on 2020-09-28 ...
Die Vermehrung von Streptomyces albus unter Nutzung von Stoffwechselendprodukten und Leibessubstanzen einer absterbenden Para-B-Kultur führt zu Rückwirkungen auf die Salmonellen. Bezogen auf den...
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Actinomadura pelletieri.
[124 Pages Report] Check for Discount on United States Bifidiobacterium Bifidum Market Report 2016 report by QYResearch Group. Notes: Sales, means the sales volume of Bifidiobacterium Bifidum Revenue,...
[Arg15, Asp16, 25, Pro18, 21, 23, Val22, Ile24]-Beta-Amyloid-Protein (15-25), 10 mg. The undecapeptide RDLPFFPVPID (iA��11) has a similar degree of hydrophobicity as the native amyloid ��-peptide (A��); but with a low propensity to adopt a
THE 38% PROTEIN Bar je slastna beljakovinska ploščica z visoko vsebnostjo sirotkinih (whey) beljakovin in nizko vsebnostjo OH ter sladkorja. Odličen zdrav posladek in nadomestek obroka.
Definition of Corynebacterium haemolyticum. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and definitions.
BACKGROUND: In the female genital tract, up to 30% of Papanicolaou (Pap)-stained cervicovaginal smears of intrauterine device (IUD) users are positive for actinomyces-like organisms (ALOs). Many clinicians believe that no therapeutic intervention is necessary if women with ALOs are without symptoms. However, there are no recommendations for the procedure in ALO-positive women with need for a routine IUD exchange. STUDY DESIGN: In this retrospective study, the incidence of ALOs was compared in ALO-positive women with a routine IUD exchange according to two new procedures: Group 1 (n=19), insertion of a new IUD immediately after removal of the index device, and Group 2 (n=19) IUD removal and reinsertion after 3-5 days. A Pap smear was obtained at intervals of 6 weeks and 12, 24 and 36 months after reinsertion. RESULTS: The cytological examination carried out after 6 weeks proved to be negative for ALOs in all cases. After 36 months, smears were more often positive for ALOs in women with immediate ...
TY - JOUR. T1 - Crystal Structures of a Glycoside Hydrolase Family 20 lacto-N-biosidase from Bifidobacterium bifidum. AU - Ito, T.. AU - Katayama, T.. AU - Hattie, M.. AU - Sakurama, H.. AU - Wada, J.. AU - Suzuki, R.. AU - Ashida, H.. AU - Wakagi, T.. AU - Yamamoto, K.. AU - Stubbs, Keith. AU - Fushinobu, S.. PY - 2013. Y1 - 2013. U2 - 10.1074/jbc.M112.420109. DO - 10.1074/jbc.M112.420109. M3 - Article. C2 - 23479733. VL - 288. SP - 11795. EP - 11806. JO - The Journal of Biological Chemistry. JF - The Journal of Biological Chemistry. SN - 0021-9258. IS - 17. ER - ...
A method for converting compactin to pravastatin is described. Compactin is provided and contacted with an agent derived from Actinomadura under conditions in which the agent converts compactin to pravastatin. Also described are an Actinomadura strain, an Actinomadura cell free extract, an Actinomadura hydroxylase, and a method for lowering cholesterol levels in mammals.
Mandelic acid (MA), an important component in pharmaceutical syntheses, is currently produced exclusively via petrochemical processes. Growing concerns over the environment and fossil energy costs have inspired a quest to develop alternative routes to MA using renewable resources. Herein we report the first direct route to optically pure MA from glucose via genetic modification of the L-phenylalanine pathway in E. coli. The introduction of hydroxymandelate synthase (HmaS) from Amycolatopsis orientalis into E. coli led to a yield of 0.092 g/L S-MA. By combined deletion of competing pathways, further optimization of S-MA production was achieved, and the yield reached 0.74 g/L within 24 h. To produce R-MA, hydroxymandelate oxidase (Hmo) from Streptomyces coelicolor and D-mandelate dehydrogenase (DMD) from Rhodotorula graminis were co-expressed in an S-MA-producing strain, and the resulting strain was capable of producing 0.68 g/L R-MA. Finally, phenylpyruvate feeding experiments suggest that HmaS is a
7-Chloro-8-methoxytetracycline isolated from antibiotic ES-119 and antibiotic Tet. 7 which are produced by fermentation of mutants of Actinomadura brunnea, namely Actinomadura brunnea var. antibiotica, ATCC 53108 and Actinomadura brunnea var antibiotica ATCC 53180 and its use as an antibiotic against gram-positive and gram-negative organisms are disclosed.
The aim of this study was to examine the influence of maternal intestinal and vaginal bifidobacteria on the establishment of bifidobacteria colonizing the gut in infants. Fecal samples from 110 healthy pregnant mothers within 1 mo before delivery and their babies at 1 mo of age and 100 vaginal swabs …
Microdialysis is a minimally invasive sampling technique that can be used to continuously monitor drug concentrations in the ECF of tissues that are accessible to microdialysis probe placement. The free drug concentration at the site of drug action in tissues is a critical determinant of the intensity of the drugs effect, and therefore monitoring tissue free drug concentration may be more informative than monitoring plasma drug concentrations.. We compared microdialysis sampling from blood and tissues to conventional sampling methods in a nonhuman primate model at steady state during a continuous infusion of zidovudine and after a bolus dose of zidovudine. The pharmacology of this nucleoside analog has been previously characterized in our animal model and the CSF penetration was 0.21 (Klecker et al., 1987; Collins et al., 1988; Balis et al., 1989). Zidovudine (mol. wt., 267) is well tolerated, lipophilic, and minimally protein bound, and a sensitive and specific assay for its detection and ...
In this symposium, Karen Kramer delivered a beautiful paper just before mine on teen pregnancy, and I had some great conversation with session participants and attendees, that has further evolved my own thinking on this issue. I want to say something just a little provocative:. While I think teen pregnancy should be avoided, culturally we overstate its dangers and consequences because we have a real problem with young people reproducing. This can lead young girls to overlook potentially more serious issues like sexually transmitted infections, HIV, and cervical cancer, all of which girls and women are at risk for if they use only hormonal contraception and have otherwise unprotected sex.. Let me explain two important points here. First, in most industrialized nations we are not set up well to support young mothers because of the way families are isolated, yet social support is a strong predictor of birth weight, postpartum depression, and labor progression (Collins et al. 1993; Feldman et al. ...
In this symposium, Karen Kramer delivered a beautiful paper just before mine on teen pregnancy, and I had some great conversation with session participants and attendees, that has further evolved my own thinking on this issue. I want to say something just a little provocative:. While I think teen pregnancy should be avoided, culturally we overstate its dangers and consequences because we have a real problem with young people reproducing. This can lead young girls to overlook potentially more serious issues like sexually transmitted infections, HIV, and cervical cancer, all of which girls and women are at risk for if they use only hormonal contraception and have otherwise unprotected sex.. Let me explain two important points here. First, in most industrialized nations we are not set up well to support young mothers because of the way families are isolated, yet social support is a strong predictor of birth weight, postpartum depression, and labor progression (Collins et al. 1993; Feldman et al. ...
Helps prevent eight clostridial diseases (including diseases caused by CI. haemolyticum and CI. perfringens Types B, C and D), which continue to be of economic importance in cattle. Clostridium bacteria are universally present and produce potent toxins that can result in rapid death of otherwise healthy animals ...
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Actinomadura coerulea.
Supplemental source of probiotics including Lactobacillus acidophilus and Bifidobacterium bifidum organisms. *These statements have not been evaluated by the Food and Drug Administration. These products are not intended to diagnose, treat, cure or prevent any disease.
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Actinomyces, aktinomycety - rod grampozitivn ch, p ev n anaerobn ch ty inkovit ch bakteri . CoJeCo.cz - Va e v eobecn encyklopedie
Solution for In each reaction box, place the best reagent and conditions from the list below.Br1)2)Br HNa, NH3 (NaNH2 (excess)ВНз/THFCH3BrNaBH4HBrH2, Lindlars…

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