Actinobacillus pleuropneumoniae: A species of gram-negative, facultatively anaerobic coccobacillus-shaped bacteria that has been isolated from pneumonic lesions and blood. It produces pneumonia with accompanying fibrinous pleuritis in swine.Actinobacillus Infections: Infections with bacteria of the genus ACTINOBACILLUS.Actinobacillus: A genus of PASTEURELLACEAE described as gram-negative, nonsporeforming, nonmotile, facultative anaerobes. Most members are found both as pathogens and commensal organisms in the respiratory, alimentary, and genital tracts of animals.Pleuropneumonia: Inflammation of the lung parenchyma that is associated with PLEURISY, inflammation of the PLEURA.Swine Diseases: Diseases of domestic swine and of the wild boar of the genus Sus.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Aggregatibacter actinomycetemcomitans: A species of Gram-negative, facultatively anaerobic spherical or rod-shaped bacteria indigenous to dental surfaces. It is associated with PERIODONTITIS; BACTERIAL ENDOCARDITIS; and ACTINOMYCOSIS.Haemophilus: A genus of PASTEURELLACEAE that consists of several species occurring in animals and humans. Its organisms are described as gram-negative, facultatively anaerobic, coccobacillus or rod-shaped, and nonmotile.Actinobacillus suis: A species of gram-negative bacteria in the genus ACTINOBACILLUS. It is mainly a pathogen of PIGS, but also can infect HORSES.Hemolysin Proteins: Proteins from BACTERIA and FUNGI that are soluble enough to be secreted to target ERYTHROCYTES and insert into the membrane to form beta-barrel pores. Biosynthesis may be regulated by HEMOLYSIN FACTORS.Serotyping: Process of determining and distinguishing species of bacteria or viruses based on antigens they share.Transferrin-Binding Proteins: A class of carrier proteins that bind to TRANSFERRIN. Many strains of pathogenic bacteria utilize transferrin-binding proteins to acquire their supply of iron from serum.Cytotoxins: Substances that are toxic to cells; they may be involved in immunity or may be contained in venoms. These are distinguished from CYTOSTATIC AGENTS in degree of effect. Some of them are used as CYTOTOXIC ANTIBIOTICS. The mechanism of action of many of these are as ALKYLATING AGENTS or MITOSIS MODULATORS.Pasteurella multocida: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria normally found in the flora of the mouth and respiratory tract of animals and birds. It causes shipping fever (see PASTEURELLOSIS, PNEUMONIC); HEMORRHAGIC BACTEREMIA; and intestinal disease in animals. In humans, disease usually arises from a wound infection following a bite or scratch from domesticated animals.Pleuropneumonia, Contagious: A pleuropneumonia of cattle and goats caused by species of MYCOPLASMA.Aspartate Ammonia-Lyase: An enzyme that catalyzes the conversion of aspartic acid to ammonia and fumaric acid in plants and some microorganisms. EC 4.3.1.1.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Pasteurellaceae: A family of coccoid to rod-shaped nonsporeforming, gram-negative, nonmotile, facultatively anaerobic bacteria that includes the genera ACTINOBACILLUS; HAEMOPHILUS; MANNHEIMIA; and PASTEURELLA.Bacterial Proteins: Proteins found in any species of bacterium.Transferrin-Binding Protein B: A subtype of bacterial transferrin-binding protein found in bacteria. It forms a cell surface receptor complex with TRANSFERRIN-BINDING PROTEIN A.Bacterial Vaccines: Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.Pasteurella: The oldest recognized genus of the family PASTEURELLACEAE. It consists of several species. Its organisms occur most frequently as coccobacillus or rod-shaped and are gram-negative, nonmotile, facultative anaerobes. Species of this genus are found in both animals and humans.Palatine Tonsil: A round-to-oval mass of lymphoid tissue embedded in the lateral wall of the PHARYNX. There is one on each side of the oropharynx in the fauces between the anterior and posterior pillars of the SOFT PALATE.Tylosin: Macrolide antibiotic obtained from cultures of Streptomyces fradiae. The drug is effective against many microorganisms in animals but not in humans.Actinobacillus equuli: A genus of gram-negative bacteria in the genus ACTINOBACILLUS, which is pathogenic for HORSES and PIGS.Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Genes, Bacterial: The functional hereditary units of BACTERIA.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Bacterial Capsules: An envelope of loose gel surrounding a bacterial cell which is associated with the virulence of pathogenic bacteria. Some capsules have a well-defined border, whereas others form a slime layer that trails off into the medium. Most capsules consist of relatively simple polysaccharides but there are some bacteria whose capsules are made of polypeptides.Iron-Binding Proteins: Proteins that specifically bind to IRON.Bacterial Toxins: Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.Lung: Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.Bacterial Outer Membrane Proteins: Proteins isolated from the outer membrane of Gram-negative bacteria.Sulfite Reductase (Ferredoxin): A FERREDOXIN-dependent oxidoreductase that is primarily found in PLANTS where it plays an important role in the assimilation of SULFUR atoms for the production of CYSTEINE and METHIONINE.Polysaccharides, Bacterial: Polysaccharides found in bacteria and in capsules thereof.Haemophilus paragallinarum: A species of gram-negative bacteria in the genus HAEMOPHILUS, causing respiratory tract disease in CHICKENS known as infectious coryza.Pasteurella Infections: Infections with bacteria of the genus PASTEURELLA.Mannheimia haemolytica: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria normally commensal in the flora of CATTLE and SHEEP. But under conditions of physical or PHYSIOLOGICAL STRESS, it can cause MASTITIS in sheep and SHIPPING FEVER or ENZOOTIC CALF PNEUMONIA in cattle. Its former name was Pasteurella haemolytica.Fibroma Virus, Rabbit: A species of LEPORIPOXVIRUS causing subcutaneous localized swellings in rabbits, usually on the feet.Phytoplasma: A genus of minute bacteria in the family ACHOLEPLASMATACEAE that inhabit phloem sieve elements of infected PLANTS and cause symptoms such as yellowing, phyllody, and witches' brooms. Organisms lack a CELL WALL and thus are similar to MYCOPLASMA in animals. They are transmitted by over 100 species of INSECTS especially leafhoppers, planthoppers, and PSYLLIDS.Hydrangea: A plant genus of the family HYDRANGEACEAE. Members contain hydrangenol, thunberginols, hydramacrosides A and B, and secoiridoid glucosides.Biofilms: Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.Thiamphenicol: A methylsulfonyl analog of CHLORAMPHENICOL. It is an antibiotic and immunosuppressive agent.Drugs, Generic: Drugs whose drug name is not protected by a trademark. They may be manufactured by several companies.Immunity, Maternally-Acquired: Resistance to a disease-causing agent induced by the introduction of maternal immunity into the fetus by transplacental transfer or into the neonate through colostrum and milk.Immunomagnetic Separation: A cell-separation technique where magnetizable microspheres or beads are first coated with monoclonal antibody, allowed to search and bind to target cells, and are then selectively removed when passed through a magnetic field. Among other applications, the technique is commonly used to remove tumor cells from the marrow (BONE MARROW PURGING) of patients who are to undergo autologous bone marrow transplantation.C-Reactive Protein: A plasma protein that circulates in increased amounts during inflammation and after tissue damage.Biological Markers: Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.Abattoirs: Places where animals are slaughtered and dressed for market.HungaryHemagglutination Tests: Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)

Pulmonary lesions in guinea pigs experimentally infected with Actinobacillus pleuropneumoniae (A.p.) serovar 1. (1/288)

Pathological studies were carried out on the lungs of guinea pigs intratracheally inoculated with 4.6 x 10(6-8) colony forming units (CFU)/head of Actinobacillus pleuropneumoniae serovar 1. All animals in the highest dose group died within 24 hr post inoculation (hpi) and showed pulmonary lesions being hemorrhagic in nature while all animals in the lowest dose group were killed as scheduled at 11 days post inoculation (dpi) and showed only hyperplasia of peribronchial lymphoid tissues. In the middle dose group, two died within 24 hpi, two died at 9 dpi, and the remaining one was killed at 11 dpi. Two guinea pigs which died at 9 dpi showed fibrinonecrotic pleuropneumonia which is the most characteristic acute pulmonary lesion in swine, and has not yet been reproduced in laboratory animals up to the present time. This suggests that guinea pigs may be a useful laboratory animal for studying the pathogenesis of Actinobacillus pleuropneumoniae infection in swine.  (+info)

Vaccination and protection of pigs against pleuropneumonia with a vaccine strain of Actinobacillus pleuropneumoniae produced by site-specific mutagenesis of the ApxII operon. (2/288)

The production of toxin (Apx)-neutralizing antibodies during infection plays a major role in the induction of protective immunity to Actinobacillus pleuropneumoniae reinfection. In the present study, the gene encoding the ApxII-activating protein, apxIIC, was insertionally inactivated on the chromosome of a serovar 7 strain, HS93. Expression of the structural toxin, ApxIIA, and of the two genes required for its secretion, apxIB and apxID, still occurs in this strain. The resulting mutant strain, HS93C- Ampr, was found to secrete the unactivated toxin. Pigs vaccinated with live HS93C- Ampr via the intranasal route were protected against a cross-serovar challenge with a virulent serovar 1 strain of A. pleuropneumoniae. This is the first reported vaccine strain of A. pleuropneumoniae which can be delivered live to pigs and offers cross-serovar protection against porcine pleuropneumonia.  (+info)

Molecular cloning and sequencing of the aroA gene from Actinobacillus pleuropneumoniae and its use in a PCR assay for rapid identification. (3/288)

The gene (aroA) of Actinobacillus pleuropneumoniae, serotype 2, encoding 5-enolpyruvylshikimate-3-phosphate synthase was cloned by complementation of the aroA mutation in Escherichia coli K-12 strain AB2829, and the nucleotide sequence was determined. A pair of primers from the 5' and 3' termini were selected to be the basis for development of a specific PCR assay. A DNA fragment of 1,025 bp was amplified from lysed A. pleuropneumoniae serotypes 1 to 12 of biovar 1 or from isolated DNA. No PCR products were detected when chromosomal DNAs from other genera were used as target DNAs; however, a 1,025-bp DNA fragment was amplified when Actinobacillus equuli chromosomal DNA was used as a target, which could be easily differentiated by its NAD independence. The PCR assay developed was very sensitive, with lower detection limits of 12 CFU with A. pleuropneumoniae cells and 0.8 pg with extracted DNA. Specificity and sensitivity make this PCR assay a useful method for the rapid identification and diagnosis of A. pleuropneumoniae infections.  (+info)

Actinobacillus pleuropneumoniae osteomyelitis in pigs demonstrated by fluorescent in situ hybridization. (4/288)

Necrotizing osteomyelitis and fibrinopurulent arthritis with isolation of Actinobacillus pleuropneumoniae serotype 2 is reported in two pigs from a herd with lameness and mild coughing problems among 8 to 12-week-old pigs. Application of fluorescent in situ hybridization targeting 16S ribosomal RNA of A. pleuropneumoniae in formalin-fixed tissue was performed to verify the association of A. pleuropneumoniae with the bone and joint lesions. By in situ hybridization A. pleuropneumoniae was demonstrated as multiple microcolonies or single cells dispersed in focal fibrinonecrotizing pleuropneumonia, in joints with arthritis, and in bone necroses including lysis of growth plate and suppurative inflammation in the adjacent trabecular metaphysis, thus demonstrating that well-known infections manifest new, unusual lesions.  (+info)

A novel enzyme-linked immunosorbent assay using the recombinant Actinobacillus pleuropneumoniae ApxII antigen for diagnosis of pleuropneumonia in pig herds. (5/288)

For the surveillance of pig herds infected with porcine pleuropneumonia, an enzyme-linked immunosorbent assay (ELISA) using the recombinant Actinobacillus pleuropneumoniae ApxII protein as species- but not serotype-specific antigen was developed. Using this ELISA, 243 of 400 animals from 22 A. pleuropneumoniae-infected herds were classified as seropositive.  (+info)

First chromosomal restriction map of Actinobacillus pleuropneumoniae and localization of putative virulence-associated genes. (6/288)

Combined physical and genetic maps of the genomes of Actinobacillus pleuropneumoniae AP76 (serotype 7 clinical isolate) and of A. pleuropneumoniae ATCC 27088 (serotype 1 reference strain) were constructed by using the restriction endonucleases ApaI, AscI, NotI, and SalI. The chromosome sizes as determined by the addition of estimated fragment sizes were 2.4 Mbp, and both maps had a resolution of approximately 100 kbp. The linkages between the ApaI, AscI, NotI, and SalI fragments and their relative positions were determined by (i) fragment excision and redigestion and (ii) partial digests of defined fragments and Southern blot using end-standing probes. The single SalI site within the chromosome of strain A. pleuropneumoniae AP76 was defined as position 1 of the map; for the map of A. pleuropneumoniae ATCC 27088, the corresponding SalI site was chosen. Putative virulence-associated genes (apx, omlA, sodA, tbpBA, ureC, and a repeat element) and housekeeping genes (glyA, metJ, recA, and rhoAP) were positioned on the physical maps and located on the ApaI and NotI fragments of A. pleuropneumoniae serotype reference strains.  (+info)

Detection of antibodies against Actinobacillus pleuropneumoniae serotypes 1, 2, 5 and 7 using the immunohistochemical staining. (7/288)

Whole cells of Actinobacillus pleuropneumoniae (A. pleuropneumoniae) serotype 1, 2, 5 or 7 attached to fibrins were fixed in 10% neutral buffered formalin and embedded in paraffin. The sections on a slide glass were stained by the avidin-biotin complex immunoperoxidase (ABC) method. Test sera were applied to sections as primary antibodies. The serum antibodies against A.pleuropneumoniae (serotypes 1, 2, 5 and 7) were measured by the ABC method and complement fixation (CF) test. There was good correlation between the ABC and CF tests. The present results indicate that the immunohistochemical staining is as useful as the CF test for the detection and quantification of antibody in swine sera.  (+info)

Characterization of apxIVA, a new RTX determinant of Actinobacillus pleuropneumoniae. (8/288)

A fourth type of RTX determinant was identified in Actinobacillus pleuropneumoniae and was designated apxIVA. When expressed in Escherichia coli, recombinant ApxIVA showed a weak haemolytic activity and co-haemolytic synergy with the sphingomyelinase (beta-toxin) of Staphylococcus aureus. These activities required the presence of an additional gene, ORF1, that is located immediately upstream of apxIVA. The apxIVA gene product could not be detected in A. pleuropneumoniae cultures grown under various conditions in vitro; however, pigs experimentally infected with A. pleuropneumoniae serotypes 1, 5 and 7 started to produce antibodies that reacted with recombinant ApxIVA 14 d post-infection, indicating that apxIVA is expressed in vivo. In addition, sera from pigs naturally and experimentally infected with any of the serotypes all reacted with recombinant ApxIVA. The apxIVA gene from the serotype 1 A. pleuropneumoniae type strain Shope 4074T encodes a protein with a predicted molecular mass of 202 kDa which has typical features of RTX proteins including hydrophobic domains in the N-terminal half and 24 glycine-rich nonapeptides in the C-terminal half that bind Ca2+. The glycine-rich nonapeptides are arranged in a modular structure and there is some variability in the number of modules in the ApxIVA proteins of different serotypes of A. pleuropneumoniae. The deduced amino acid sequences of the ApxIVA proteins have significant similarity with the Neisseria meningitidis iron-regulated RTX proteins FrpA and FrpC, and to a much lesser extent with other RTX proteins. The apxIVA gene could be detected in all A. pleuropneumoniae serotypes and seems to be species-specific. Although the precise role of this new RTX determinant in pathogenesis of porcine pleuropneumonia needs to be determined, apxIVA is the first in vivo induced toxin gene that has been described in A. pleuropneumoniae.  (+info)

TY - JOUR. T1 - Actinobacillus pleuropneumoniae serotypes in Hungary. AU - Sárközi, Rita. AU - Makrai, L.. AU - Fodor, L.. PY - 2018/9/1. Y1 - 2018/9/1. N2 - A total of 255 Actinobacillus pleuropneumoniae isolates were collected from 634 lung samples representing 70 swine herds in Hungary between January 2012 and June 2016. On the basis of the indirect haemagglutination test 77 independent strains were included in the evaluation after the elimination of duplicate or multiple serotypes from the same herd. In the case of 7 herds strains of two different serotypes were identified. Fourteen Hungarian A. pleuropneumoniae isolates from the culture collection of the Department of Microbiology and Infectious Diseases, isolated before 2012, were also included in the evaluation (one each from 12 herds and two each from two herds, where two serotypes occurred). Out of the altogether 91 A. pleuropneumoniae strains 72 strains belonged to biotype I and 19 strains could be allocated to biotype II. In ...
In Denmark porcine pleuropneumonia is most frequently caused by Actinobacillus pleuropneumoniae serotype 2 (60%). Isolation of A. pleuropneumoniae from nasal cavities or tonsils from carrier animals is complicated due to the mixed bacterial flora present. An immunomagnetic separation technique (IMS) using immunomagnetic beads (Dynabeads(R)) was developed for isolation of A. pleuropneumoniae serotype 2 from pure cultures and from heterogeneous suspensions. Different coating and washing procedures were evaluated in pure and mixed cultures using polyclonal (PAb) and monoclonal antibodies. The highest reisolation yield was achieved when the beads were coated with 1.5 mug PAb IgG/10(7) beads. After washing the beads for four times 9-24% of the bacteria could be reisolated depending on the amount of IgG attached to the beads and the number of beads used. The recovery was increased to 19-61% when only two washing steps were performed. The IMS was further evaluated using dilutions of A. pleuropneumoniae ...
Actinobacillus pleuropneumoniae serotype 3 UPF0263 protein APJL_1366 (APJL_1366) datasheet and description hight quality product and Backed by our Guarantee
Serotypes 3 and 8 of Actinobacillus pleuropneumoniae, the aetiological agent of porcine pleuropneumonia, have been reported to predominate in the UK. Direct serotyping of isolates of the organism is typically determined by the immunological reactivity of rabbit serum to its surface polysaccharides, but the method has limitations, for example, cross-reactions between serotypes 3, 6 and 8. This study describes the development of a serotype 3-specific PCR, based on the capsule locus, which can be used in a multiplex format with the organisms specific gene apxIV. The PCR test was evaluated on 266 strains of A pleuropneumoniae and 121 strains of other organisms, including all the major respiratory bacterial pathogens of pigs. The test was highly specific and sensitive and should be useful for differentiating strains of serotypes 3, 6 and 8, and in seroprevalence and epidemiological surveys in regions where serotype 3 is prevalent, such as the UK.. ...
article{176028, author = {Haesebrouck, Freddy and Chiers, Koen and Van Overbeke, Ingrid and Ducatelle, Richard}, issn = {0378-1135}, journal = {VETERINARY MICROBIOLOGY}, language = {eng}, number = {2-4}, pages = {239--249}, title = {Actinobacillus pleuropneumoniae infections in pigs: the role of virulence factors in pathogenesis and protection.}, volume = {58}, year = {1997 ...
Actinobacillus pleuropneumoniae (previously Haemophilus pleuropneumoniae), is a Gram-negative, facultative anaerobic, respiratory pathogen found in pigs. It was first reported in 1957, and was formally declared to be the causative agent of porcine pleuropneumonia in 1964. It was reclassified in 1983 after DNA studies showed it was more closely related to A. lignieresii. A. pleuropneumoniae is a nonmotile, Gram-negative, encapsulated coccobacillus bacterium found in the Pasteurellaceae family. It exhibits β-hemolysis activity, thus explaining its growth on chocolate or blood agar, but must be supplemented with NAD (V factor) to facilitate growth for one of its biological variants (biovar 1). As a facultative anaerobic pathogen, A. pleuropneumoniae may need CO2 to grow. Depending on the biovar, the bacteria may or may not be positive for urease; both biovars are positive for porphyrin. A. pleuropneumoniae was found to be the causative agent for up to 20% of all bacterial pneumonia cases in ...
Fifty randomly selected fattening pig herds were studied to investigate the epidemiological characteristics of infections with Actinobacillus pleuropneumoniae serovars 2, 3 and 9, and to identify risk factors for their within-herd seroprevalences. Information about 13 farm characteristics was obtained by means of a questionnaire and used to assess potential risk factors for the percentage of slaughter pigs with antibodies against each of the three serovars. The presence of antibodies was measured with an indirect ELISA. The median within-herd seroprevalence for serovar 2 was 58 per cent (range 0 to 100 per cent), for serovar 3, 53 per cent (range 10 to 95 per cent), and for serovar 9, 35 per cent (range 5 to 100 per cent). All but one farm tested positive for A pleuropneumoniae serovar 2, and all the farms were positive for A pleuropneumoniae serovars 3 and 9. There was a positive association (P<0.05) between each pair of serovars. The within-herd seroprevalence of serovar 2 was ...
Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, a significant disease that causes serious economic losses to the swine industry worldwide. Persistent infections caused by bacterial biofilms are recalcitrant to treat because of the particular drug resistance of biofilm-dwelling cells. TolC, a key component of multidrug efflux pumps, are responsible for multidrug resistance in many Gram-negative bacteria. In this study, we identified two TolC-like proteins, TolC1 and TolC2, in A. pleuropneumoniae. Deletion of tolC1, but not tolC2, caused a significant reduction in biofilm formation, as well as increased drug sensitivity of both planktonic and biofilm cells. The genetic-complementation of the tolC1 mutation restored the competent biofilm and drug resistance. Besides, biofilm formation was inhibited and drug sensitivity was increased by the addition of phenylalanine-arginine beta-naphthylamide (PAβN), a well-known efflux pump inhibitor (EPI), suggesting a role
0004] It is commonly known how to produce the RTX-toxin ApxI by culturing Actinobacillus pleuropneumoniae in a culturing medium to which a calcium salt (i.e. a chemical compound, based on an acid, formed by replacing all or part of the hydrogen ions of the acid with one or more calcium ions) is added. In particular, EP 0 453 024 already describes such a method (see "Example 2", paragraph 2 "Purification and characterisation of hemolysin", subparagraph "Methods"). Note that ApxI used to be referred to as "HLY" (see Frey et al. in "J Gen Microbiol. 1993 August; 139(8): 1723-8"). From this EP patent it is known to add a calcium compound (CaCl2) to the medium. Indeed, in Microbiol Pathogenesis 37 (2004) 29-33 it is stated that transcriptional activity of the ApxI operon is enhanced by addition of calcium to the growth medium. This way, high levels of ApxI can be provided for. The medium should support the growth of APP bacteria. It is commonly known how to constitute a medium that supports growth of ...
Actinobacillus pleuropneumoniae ApaA protein: an antigenic membrane protein from Actinobacillus pleuropnemoniae homology to ABC transporters; amino acid sequence in first source
Pleuropneumonia infectioasa a porcului (PIP), cauzata de Actinobacillus pleuropneumoniae (App), poate fi intalnita la porcii de toate varstele, inclusiv la mistreti, dar simptomele clinice cele mai frecvente sunt inregistrate la porcii in faza de finisare, in varsta de peste 12 saptamani. In formele clasice de boala animalele manifesta febra si simptome respiratorii, datorate leziunilor caracteristice de pneumonie hemoragico-necrotica sau pleuropneumonie fibrinoasa. Boala poate evolua supra-acut, fara semne clinice, animalele fiind gasite moarte, acut, mai ales in efectivele naive imunologic, cu pierderi prin mortalitate de 15-20%, sau sub-acut, cu mortalitate mai redusa dar variabila. In efectivele infectate endemic boala se cronicizeaza la animalele care au supravietuit, afectand starea lor de bine si producand o scadere a performantelor zootehnice. Porcii pot fi purtatori fara sa dezvolte vreun raspuns imun. Boala genereaza pierderi economice insemnate.. ...
Biomarkers of infection were screened for their possible role as evaluators of antibiotic treatment in an aerosol infection model of porcine pneumonia caused by Actinobacillus pleuropneumoniae (Ap). Following infection of 12 pigs, clinical signs of pneumonia developed within 20 h, whereafter the animals received a single dose of either danofloxacin (2.5 mg/kg) or tiamulin (10 mg/kg). To test the discriminative properties of the biomarkers, the dosage regimens were designed with an expected difference in therapeutic efficacy in favour of danofloxacin. Accordingly, the danofloxacin-treated pigs recovered clinically within 24h after treatment, whereas tiamulin-treated animals remained clinically ill until the end of the study, 48 h after treatment. A similar Picture was seen for the biomarkers of infection. During the infection period, plasma C-reactive protein (CRP), interleukin-6 and haptoglobin increased, whereas plasma zinc, ascorbic acid and alpha-tocopherol decreased. In the ...
MUNOZ, D; QUEZADA, M y RUIZ, A. Serological behaviour study of Actinobacillus pleuropneumoniae (App) in commercial swine herds from the central region of Chile. Arch. med. vet. [online]. 2008, vol.40, n.2, pp.147-153. ISSN 0301-732X. http://dx.doi.org/10.4067/S0301-732X2008000200006.. In Chile, there was only one existing study on App. This study was designed to determine the maternal immunity duration, the age of seroconversion and the apparent and true prevalence in animals from 7 swine commercial herds. 60 samples were taken per herd and divided into 10 serum samples from animals of 4, 6,10,14,18and21 weeks of age, which were analyzed by ELISA®. Out of the 420 samples, 134 were positive with 112 of them belonging to pigs under 10 weeks of age while only 22 were from animals over 10 weeks of age, which seroconverted presumably due to a natural infection. Regarding maternal immunity duration it was found that around the 10th week of age the animals lose their colostrum antibodies. In ...
Actinobacillus pleuropneumoniae ATCC ® 33590™ Designation: Femo TypeStrain=True Application: Characterization Respiratory research
Actinobacillus pleuropneumoniae, a Gram-negative, non-motile, facultatively anaerobic Pasteurellaceae, causes porcine pleuropneumonia, a highly contagious disease for which there is no effective vaccine. There are least twelve different serotypes, some of which produce no disease but others cause severe disease. Serotypes vary in different countries. Types 1, 5, 9, 11 and 12 are usually highly virulent and strains 3 and 6 are mild. The bacterium is carried in the tonsils and upper respiratory tract. It is transmitted short distances by droplet infection and only survives outside the pig for a few days.Actinobacillus pleuropneumoniae strain JL03 is an isolate of serotype 3 which is prevalent in China. Its genome is made up of a single circular chromosome encoding 2097 CDSs. It possesses a complete set of genes coding for enzymes of glycolysis, gluconeogenesis and non-oxidative pentose phosphate pathways. Genes coding for the key enzymes of the TCA cycle, citrate synthase, aconitase and isocitrate ...
ID ACTP7_1_PE100 STANDARD; PRT; 215 AA. AC ACTP7_1_PE100; B3GZU0; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Cytochrome c-type protein NrfB; (ACTP7_1.PE100). GN Name=nrfB; OrderedLocusNames=APP7_0100; OS ACTINOBACILLUS PLEUROPNEUMONIAE SEROVAR 7 STR. AP76. OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; OC Pasteurellaceae; Actinobacillus. OX NCBI_TaxID=537457; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS ACTP7_1.PE100. CC Actinobacillus pleuropneumoniae serovar 7 str. AP76, complete genome. CC sequence. CC -!- ANNOTATIONS ORIGIN:B3GZU0_ACTP7 CC -!- GENE_FAMILY: HOG000278525 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; B3GZU0; -. DR EMBL; CP001091; ACE60752.1; -; Genomic_DNA. DR RefSeq; YP_001967894.1; NC_010939.1. DR ProteinModelPortal; B3GZU0; -. DR STRING; B3GZU0; -. DR GeneID; 6397435; -. DR GenomeReviews; CP001091_GR; APP7_0100. DR ...
Shipping (test code: xtrnc) and handling (test code: xhand) fees are also applicable on each submission. External test price is subject to change. ...
Pharmacokinetic-pharmacodynamic (PK/PD) integration and modelling were used to predict dosage schedules for florfenicol for two pig pneumonia pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida. Pharmacokinetic data were pooled for two bioequivalent products, pioneer and generic formulations, administered intramuscularly to pigs at a dose rate of 15 mg/kg. Antibacterial potency was determined in vitro as minimum inhibitory concentration (MIC) and Mutant Prevention Concentration in broth and pig serum, for six isolates of each organism. For both organisms and for both serum and broth MICs, average concentration:MIC ratios over 48 h were similar and exceeded 2.5:1 and times greater than MIC exceeded 35 h. From in vitro time-kill curves, PK/PD modelling established serum breakpoint values for the index AUC24h/MIC for three levels of inhibition of growth, bacteriostasis and 3 and 4log10 reductions in bacterial count; means were 25.7, 40.2 and 47.0 h, respectively, for P. multocida and 24.6,
SWISS-MODEL Repository entry for B3GZ69 (KTHY_ACTP7), Thymidylate kinase. Actinobacillus pleuropneumoniae serotype 7 (strain AP76)
Complete article is available online.. PDF version is available online.. Objective: To evaluate the duration of efficacy of a single dose of ceftiofur crystalline free acid sterile suspension (CCFA-SS) against clinical disease in grower pigs inoculated intratracheally with Actinobacillus pleuropneumoniae.. Materials and methods: One hundred and ninety male piglets were blocked by weight and randomly assigned to 19 pens of 10 animals each, with three pens randomly assigned to each of six treatments. Pigs were challenged with A pleuropneumoniae at 7 to 8 weeks old (Day 0). Groups 1 through 5 were treated with a single intramuscular dose of CCFA-SS (5 mg ceftiofur equivalents per kg body weight) on Days -13, -10, -7, -4, and -1, respectively. Group 6 pigs were challenged, untreated controls. A single pen of 10 unchallenged, untreated pigs acted as sentinels. The primary variable was removal rate (percent of pigs per pen that died or were euthanized because of severe illness by Day 9). Ancillary ...
Pourquier, P., Lesceu, S. Use of the ID Screen® APP indirect ELISA range for quick identification and serotyping of Actinobacillus pleuropneumoniae in domestic pigs. Poster presented at the 6th International Symposium on Emerging and Re-emerging Pig Diseases, June 2011, Madrid, Spain ...
Formulated to provide outstanding protection against the most common and virulent serotypes 1, 5 and 7. For prevention of pneumonia in pigs 4 weeks of age or older by infection with the prevalent serotypes of Actinobacillus pleuropneumoniae ...
Porcine pleuropneumonia, caused by Actinobacillus pleuropneumoniae, is a bacterial respiratory disease of swine. Acute outbreaks of the disease are often accompanied by high mortality and economic losses. As severe cases of the disease frequently require parenteral antibiotic treatment of the animals, the efficacy of a single, high dose of marbofloxacin was compared to a three-time application of a dose of enrofloxacin under experimental conditions. A blinded, controlled, randomized and blocked dose confirmation study was conducted to test the efficacy and safety of a single dose of 8 mg/kg marbofloxacin (160 mg/ml, Forcyl® Swine, Vetoquinol SA, France) to treat acute porcine pleuropneumonia after experimental aerosol inoculation of pigs with A. pleuropneumoniae serotype 2. The results were compared to a three consecutive day treatment of 2.5 mg/kg enrofloxacin and a mock (saline) treatment. Criteria for the assessment of efficacy were severity of lung lesions, bacteriological cure and the course of
LIU, Qiong et al. Construction and immunogenicity of a ΔapxIC/ompP2 mutant of Actinobacillus pleuropneumonias and Haemophilus parasuis. Onderstepoort j. vet. res. [online]. 2013, vol.80, n.1, pp.1-6. ISSN 2219-0635.. The apxIC genes of the Actinobacillus pleuropneumoniae serovar 5 (SC-1), encoding the ApxI-activating proteins, was deleted by a method involving sucrose counter-selection. In this study, a mutant strain of A. pleuropneumoniae (SC-1) was constructed and named AapxIC/ ompP2. The mutant strain contained foreign DNA in the deletion site of ompP2 gene of Haemophilus parasuis. It showed no haemolytic activity and lower virulence of cytotoxicity in mice compared with the parent strain, and its safety and immunogenicity were also evaluated in mice. The LD50 data shown that the mutant strain was attenuated 30-fold, compared with the parent strain (LD50 of the mutant strain and parent strain in mice were determined to be 1.0 x 107 CFU and 3.5 x 105 CFU respectively). The mutant strain that ...
The complete nucleotide sequence of a 7.7kb mobilisable plasmid (pM3446F), isolated from a florfenicol resistant isolate of Actinobacillus pleuropneumoniae, showed extended similarity to plasmids found in other members of the Pasteurellaceae containing the floR gene as well as replication and mobilisation genes. Mobilisation into other Pasteurellaceae species confirmed that this plasmid can be transferred horizontally ...
TY - JOUR. T1 - Optimal combinations of acute phase proteins for detecting infectious disease in pigs. AU - Heegaard, Peter M. H. AU - Stockmarr, Anders AU - Piñeiro, Matilde AU - Carpintero, Rakel AU - Lampreave, Fermin AU - Campbell, Fiona Margaret. AU - Eckersall, P. David AU - Toussaint, Mathilda J.M. AU - Gruys, Erik AU - Skall Sorensen, Nanna PY - 2011/3/17. Y1 - 2011/3/17. N2 - The acute phase protein (APP) response is an early systemic sign of disease, detected as substantial changes in APP serum concentrations and most disease states involving inflammatory reactions give rise to APP responses. To obtain a detailed picture of the general utility of porcine APPs to detect any disease with an inflammatory component seven porcine APPs were analysed in serum sampled at regular intervals in six different experimental challenge groups of pigs, including three bacterial (Actinobacillus pleuropneumoniae, Streptococcus suis, Mycoplasma hyosynoviae), one parasitic (Toxoplasma gondii) and one ...
Welcome to the Inform a Colleague area of the Academy Website. The American Academy of Spine Physicians (AASP) strives to implement new and more efficient methods for members to communicate with other healthcare professionals. One of the most effective tools is the online "Inform a Colleague" function. This function can be used to expand and educate your spinecare referral network. It also represents a resource to conveniently inform others about trends in spinecare and about offers of organizations such as the American Academy of Spine Physicians (AASP), the International Spine Association (ISA). The link library also contains an application for membership with the AASP ...
Patrick G. Halbur and Eric Bush. Report: Results of the recent survey of the membership of the AASP for outbreaks of sow abortion and mortality ...
Yeah - I dont think its all one or the other - but what reason to not expect the worst administration in history (@ least our own)? Theres already damage to civil society - & his cabinet is full of Oligarchs & people determined to destroy the very departments theyre supposed to run. Hes surrounding himself w/ Generals & dispensing w/ Diplomacy...Were that it was only ridiculous. The country & the world are @ critical junctures - & the reins are being handed to a hate-filled racist nationalist business fraud, bankruptcy pest, & game show host famous for terminating people. But - this is not Unreality TV - this is the real stage ...
Structural studies of the O-chain of the phenol-phase soluble lipopolysaccharide from Haemophilus pleuropneumoniae serotype 2: Biochem.Cell Biol.
TY - JOUR. T1 - Effect of heat treatment on the surface antigens of Haemophilus pleuropneumoniae.. AU - Mittal, K. R.. AU - Higgins, Robert. AU - Larivière, S.. AU - Martineau, G. P.. PY - 1987/1/1. Y1 - 1987/1/1. N2 - Coagglutination and ring precipitation tests were used to study the effect of heat on the surface antigens of Haemophilus pleuropneumoniae strains employing the reference strains belonging to serotypes 1 to 7 and field isolates belonging to serotypes 1, 2, 3, 5, 6 and 7. By immunising rabbits with formalin-fixed whole-cell suspension, antibodies were obtained which sensitised Cowan I Staphylococcus aureus to coagglutinate antigen preparations which had not been heated, or heated at 56 degrees C, or boiled or autoclaved. Similar positive reactions were obtained with the ring precipitation test. Heating the cultures at 56 degrees C for one hour was best for exposing the most potent serotype-specific antigens in all the strains studied. All the reference strains and most of the ...
Looking for Actinobacillus capsulatus? Find out information about Actinobacillus capsulatus. A genus of gram-negative, immotile and nonspore-forming, oval to rod-shaped, often pleomorphic bacteria which occur as parasites or pathogens in mammals ,... Explanation of Actinobacillus capsulatus
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Actinobacillus hominis.
Actinobacillus equulis: especie que se encuentra en la cavidad bucal y amígdalas del caballo y causa lesiones supurativas de los riñones y articulaciones de los cerdos y caballos y endocarditis en el cerdo ...
Learn about the veterinary topic of Pleuropneumonia in Horses. Find specific details on this topic and related topics from the Merck Vet Manual.
Oral fluid analysis for herd monitoring is of interest to the commercial pig production in Korea. The aim of this study was to investigate pathogen-positive rates and correlations among eight pathogens associated with porcine respiratory disease complex by analyzing oral fluid samples from 214 pig groups from 56 commercial farms. Samples collected by a rope-chewing method underwent reverse-transcriptase polymerase chain reaction (RT-PCR) or standard polymerase chain reaction (PCR) analysis, depending on the microorganism. Pathogens were divided into virus and bacteria groups. The former consisted of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 (PCV2), and the latter Pasteurella multocida, Haemophilus parasuis, Actinobacillus pleuropneumoniae, Mycoplasma hyopneumoniae (MHP), Mycoplasma hyorhinis, and Streptococcus suis (SS). All pathogens were detected more than once by PCR. Age-based analysis showed the PCR-positive rate increased with increasing age for PCV2 ...
Periodontitis is mankinds most common chronic inflammatory disease. One severe form of periodontitis is localized aggressive periodontitis (LAP), a condition to which individuals of African origin demonstrate an increased susceptibility. The main causative organism of this disease is Actinobacillus …
The use of the porcine lung to demonstrate recruitment maneuvers stimulated us to consider the possibility of using the ASL 5000 to investigate ways to improve protocols for the handling of ex-vivo perfused lungs. We believe this would provide interesting research projects for students and faculty. Many of the current ex-vivo perfusion protocols involve using positive pressure and high concentrations of oxygen to maintain isolated perfused lungs. The longest time that perfused lungs have been kept viable ex-vivo is about 10 hours. Using the ASL 5000 to model breathing, we would like to explore the possibility of extending survival time of ex-vivo perfused lungs. We believe that lowering the FiO2 and avoiding positive pressure ventilation could improve viability. Further development of this model could lead to advances in therapeutics related to airway clearance, mechanical ventilation, and airway pharmacology.. In the video below, the porcine lungs are attached to an Avea ventilator in the CPAP ...
The assumption that USS arise by mutation rather than copying was first confirmed using alignments of H. influenzae proteins with distant homologues. Abundant USS-like repeats were found in all eight Pasteurellacean genomes; the repeat consensuses of species in the Hin subclade were identical to that of H. influenzae (AAGTGCGGT), whereas members of the Apl subclade shared the consensus ACAAGCGGT. All species USSs had the strong consensus and flanking AT-rich repeats of H. influenzae USSs. DNA uptake and competition experiments demonstrated that the Apl-type repeat is a true USS distinct from the Hin-type USS: A. pleuropneumoniae preferentially takes up DNA fragments containing the Apl-type USS over both H. influenzae and unrelated DNAs, and H. influenzae prefers its own USS over the Apl type ...
TY - GEN. T1 - Experimental and modelling studies of the bioconversion of glycerol to succinic acid by actinobacillus succinogenes. AU - Vlysidis, Anestis. AU - Du, Chenyu. AU - Webb, Colin. AU - Theodoropoulos, Constantinos. PY - 2008/12/1. Y1 - 2008/12/1. N2 - An unstructured kinetic model for the growth of Actinobacillus succinogenes on glycerol as the only carbon source is proposed in this study. The model describes cell growth considering both substrate and product inhibition. and its parameters are estimated by fitting the model predictions to experimental data. Substrate consumption and product formation rates are described by the Luedeking-Piret model. The main product of the process is succinic acid while by-products like acetate, formate and ethanol have very low concentrations. The main environmental factors that affect the bioprocess were examined and optimum conditions in terms of yield, final succinic acid concentration and productivity were evaluated by a factorial experimental ...
A little over two years ago, we commented on a general rise in the frequency of Actinobacillus suis (A. suis) isolation from our swine tissue submission cases, and wondered if it was related to the increased porcine circovirus type 2 (PCV2) activity (National Hog Farmer North American Preview, June 6, 2008).
Structures of the antigenic O-polysaccharides of lipopolysaccharides produced by Actinobacillus actinomycetemcomitans serotypes a, c, d and e
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0165] (1) Wilson M, Henderson, B. Virulence factors of A. actinomycetemcomitans revenant to the pathogenesis of inflammatory periodontal diseases. FEMS 1995:17:365-379. [0166] (2) Nowotny A, Behling U H, Hammond B, et al. Release of toxic microvesicles by Actinobacillus actinomycetemcomitans. Infect Immun 1982:37:151-154. [0167] (3) Kaplan J B, Perry M B, MacLean L L, Furgang D, Wilson M E, Fine D H. Structural and genetic analyses of 0 polysaccharide from Actinobacillus actinomycetemcomitans serotype f. Infect Immun 2001:69:5375-5384. [0168] (4) Rosan B, Slots J, Lamont R J, Listgarten M A, Nelson G M. Actinobacillus actinomycetemcomitans fimbriae. Oral Microbiol Immunol 1988:3:58-63. [0169] (5) Inouye T, Ohta H, Kokeguchi S, Fukui K, Kato K. Colonial variation and fimbriation of Actinobacillus actinomycetemcomitans. FEMS microbiology letters 1990: 57: 13-17. [0170] (6) Kaplan J B, Schreiner H C, Furgang D, Fine D H. Population structure and genetic diversity of Actinobacillus ...
Succinic acid (SA) is poised to become a significant building-block or platform chemical in the bio-based economy. Of the microbial strains that show promise for biological SA production, the wild-type bacterium Actinobacillus succinogenes is one of the top-contenders. While strides have been made towards understanding the behaviour of the organism and developing the fundamentals for an industrial process based on the organism, there is still a large scope of research required and a multitude of challenges to be addressed. In particular, an improved understanding of the metabolism of the organism under favourable biofilm conditions is required and its potential as a microbial host in a biorefinery setting needs to be established. Therefore, the aim of this thesis is to develop a fundamental understanding of the central metabolism of the organism under biofilm conditions on relevant substrates, and to determine its performance as a SA producer on scalable biorefinery streams. Continuous operation ...
A novel biosimulator based on ex vivo porcine lungs for training in peripheral tissue sampling using endobronchial ultrasonography with a guide sheath
Modun, Belinda, and Paul Williams. "The Staphylococcal Transferrin-Binding Protein Is a Cell Wall Glyceraldehyde-3-Phosphate Dehydrogenase." Infection and Immunity 67.3 (1999): 1086-1092. Web. 29 Mar. 2020. ...
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Acinetobacter baumannii Actinobacillus pleuropneumoniae Anaplasma phagocytophilum Bacillus anthracis Bacillus cereus Bartonella henselae Bartonella quintana Bordetella bronchiseptica Bordetella pertussis Borrelia burgdorferi Brucella abortus Brucella canis Burkholderia pseudomallei Campylobacter jejuni Capnocytophaga canimorsus Chlamydia pneumoniae Chlamydia trachomatis Chlamydophila psittaci Clostridium botulinum Clostridium tetani Clostridium perfringens Corynebacterium pseudotuberculosis Coxiella burnetii Cronobacter sakazakii Francisella tularensis Haemophilus influenzae Haemophilus parasuis Helicobacter pylori…
"Development of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae infection". Vaccine. 24 (49): 7226-32. doi: ... Actinobacillus pleuropneumonia and Salmonella infections in pigs. Vaccine development has several trends: Until recently,[when ...
"Poly-N-acetylglucosamine mediates biofilm formation and antibiotic resistance in Actinobacillus pleuropneumoniae". Microb ... Kaplan JB, Ragunath C, Ramasubbu N, Fine DH (August 2003). "Detachment of Actinobacillus actinomycetemcomitans biofilm cells by ... Kaplan JB, Meyenhofer MF, Fine DH (2003). "Biofilm growth and detachment of Actinobacillus actinomycetemcomitans". J. Bacteriol ... a biofilm-releasing glycoside hydrolase from the periodontopathogen Actinobacillus actinomycetemcomitans". J. Mol. Biol. 349 (3 ...
Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae are pathogens that cause respiratory disease in swine. Haemophilus ... coli TOP10 strain Campylobacter jejuni Mycoplasma hyopneumoniae Haemophilus influenzae Actinobacillus pleuropneumoniae ...
Clade II, encompassing Actinobacillus pleuropneumoniae, Actinobacillus minor, Haemophilus ducreyi, Mannheimia haemolytica and ... Two major clades are formed within this Pasteurellales: Clade I, encompassing Aggregatibacter, Pasteurella, Actinobacillus ...
... reducing pig deaths and disease from the bacteria Actinobacillus pleuropneumoniae. The Molecular Plant Breeding CRC developed ...
Actinobacillus pleuropneumoniae, and Streptococcus suis. Mastitis-metritis-agalactia syndrome involved with E. coli, ...
... while the other includes Actinobacillus minor, Actinobacillus pleuropneumoniae, Haemophilus ducreyi, Haemophilus parasuis, and ... The distribution of CSIs corresponds to sensu stricto clades of "true" Actinobacillus, Haemophilus, and Pasteurella species, ... and Actinobacillus species. Comparative analyses of Pasteurellaceae genomes have identified large numbers (>20) of conserved ... Actinobacillus, Haemophilus, and Pasteurella. These genera demonstrate extensive polyphyly across the family, however, CSIs ...
Actinobacillus equuli MeSH B03.440.450.009.580 --- Actinobacillus pleuropneumoniae MeSH B03.440.450.009.675 --- Actinobacillus ... Actinobacillus equuli MeSH B03.660.250.550.050.580 --- Actinobacillus pleuropneumoniae MeSH B03.660.250.550.050.675 --- ... Actinobacillus seminis MeSH B03.660.250.550.050.700 --- Actinobacillus suis MeSH B03.660.250.550.290 --- Haemophilus MeSH ... Actinobacillus MeSH B03.660.250.550.050.050 --- Actinobacillus actinomycetemcomitans MeSH B03.660.250.550.050.200 --- ...
2008). "Actinobacillus pleuropneumoniae". Pasteurellaceae: Biology, Genomics and Molecular Aspects. Caister Academic Press. ... Actinobacillus (Pasteurella) ureae and A. hominis occur in the respiratory tracts of healthy humans and may be involved in the ... Actinobacillus is a genus of Gram-negative, nonmotile and nonspore-forming, oval to rod-shaped bacteria occurring as parasites ... ISBN 978-1-904455-24-0. Nørskov-Lauritsen N, Kilian M (September 2006). "Reclassification of Actinobacillus ...
Streptococcus suis and Actinobacillus pleuropneumoniae. Antibiotics such as ceftiofur, gentamicin and trimethoprim/sulfadiazine ... Actinobacillus suis is a beta-haemolytic, Gram-negative bacterium of the Pasteurellaceae family. The bacterium has many strains ... Type strain of Actinobacillus suis at BacDive - the Bacterial Diversity Metadatabase. ... Actinobacillus suis, reviewed and published by Wikivet at http://en.wikivet.net/Actinobacillus_suis accessed 07/10/2011. ...
... (previously Haemophilus pleuropneumoniae), is a Gram-negative, facultative anaerobic, ... Gillaspy, A. "Actinobacillus pleuropneumoniae". Laboratory for Molecular Biology and Cytometry Research-University of Oklahoma ... "Actinobacillus pleuropneumoniae". Iowa State University College of Veterinary Medicine. Retrieved 20 April 2013. [permanent ... Brownfield, B. "Actinobacillus pleuropneumoniae in swine". Purdue University-Animal Disease Diagnostic Laboratory. Retrieved 20 ...
"Development of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae infection". Vaccine. 24 (49-50): 7226-37. doi: ... Actinobacillus pleuropneumonia[111] and Salmonella infections in pigs.[112] ...
Isolation of Actinobacillus pleuropneumoniae serotype 2 by immunomagnetic separation. Publication: Research - peer-review › ... In Denmark porcine pleuropneumonia is most frequently caused by Actinobacillus pleuropneumoniae serotype 2 (60%). Isolation of ... By using IMS A. pleuropneumoniae serotype 2 could be reisolated from the tonsils of three pigs. The LMS method represents a ... From 12 pigs inoculated with an aerosol of A. pleuropneumoniae serotype 2 the bacterium could not be detected from the nasal ...
title = "Actinobacillus pleuropneumoniae serotypes in Hungary",. abstract = "A total of 255 Actinobacillus pleuropneumoniae ... Actinobacillus pleuropneumoniae serotypes in Hungary. / Sárközi, Rita; Makrai, L.; Fodor, L.. In: Acta Veterinaria Hungarica, ... Sárközi, Rita ; Makrai, L. ; Fodor, L. / Actinobacillus pleuropneumoniae serotypes in Hungary. In: Acta Veterinaria Hungarica. ... Sárközi, R., Makrai, L., & Fodor, L. (2018). Actinobacillus pleuropneumoniae serotypes in Hungary. Acta Veterinaria Hungarica, ...
Actinobacillus pleuropneumoniae comb. nov.) on the basis of phenotypic and deoxyribonucleic acid relatedness. Int J Syst ... Reclassification of equine isolates previously reported as Actinobacillus equuli, variants of A. equuli, Actinobacillus suis or ... Pohl, S., Bertschinger, H. U., Frederiksen, W. & Mannheim, W. ( 1983 ). Transfer of Haemophilus pleuropneumoniae and the ... Christensen, H. & Bisgaard, M. ( 2004 ). Revised definition of Actinobacillus sensu stricto isolated from animals: a review ...
Actinobacillus pleuropneumoniae (previously Haemophilus pleuropneumoniae), is a Gram-negative, facultative anaerobic, ... Gillaspy, A. "Actinobacillus pleuropneumoniae". Laboratory for Molecular Biology and Cytometry Research-University of Oklahoma ... "Actinobacillus pleuropneumoniae". Iowa State University College of Veterinary Medicine. Retrieved 20 April 2013. [permanent ... Brownfield, B. "Actinobacillus pleuropneumoniae in swine". Purdue University-Animal Disease Diagnostic Laboratory. Retrieved 20 ...
Shipping (test code: xtrnc) and handling (test code: xhand) fees are also applicable on each submission. External test price is subject to change. ...
Actinobacillus pleuropneumoniae L20. ›Actinobacillus pleuropneumoniae serovar 5b str. L20. ›Actinobacillus pleuropneumoniae ... Actinobacillus. › Actinobacillus pleuropneumoniae. › Actinobacillus pleuropneumoniae serovar 5. Strains i. › L20. See also. › ... Actinobacillus pleuropneumoniae serotype 5b (strain L20). Taxonomy navigation. › Actinobacillus pleuropneumoniae serovar 5 ...
Characterization of apxIVA, a new RTX determinant of Actinobacillus pleuropneumoniae.. Schaller A1, Kuhn R, Kuhnert P, Nicolet ... A fourth type of RTX determinant was identified in Actinobacillus pleuropneumoniae and was designated apxIVA. When expressed in ... The apxIVA gene could be detected in all A. pleuropneumoniae serotypes and seems to be species-specific. Although the precise ... The apxIVA gene from the serotype 1 A. pleuropneumoniae type strain Shope 4074T encodes a protein with a predicted molecular ...
Genomic DNA from Actinobacillus pleuropneumoniae 4074 TypeStrain=True Application: ... Actinobacillus pleuropneumoniae (Shope) Pohl et al. (ATCC® 27088D-5™) Strain Designations: Genomic DNA from Actinobacillus ... Genomic DNA from Actinobacillus pleuropneumoniae 4074 [ATCC® 27088™] Biosafety Level 1 Biosafety classification is based on U.S ... Nucleotide (GenBank) : AACK01000000 Actinobacillus pleuropneumoniae serovar 1 str. 4074, whole genome shotgun sequencing ...
Actinobacillus pleuropneumoniae ATCC ® 33590™ Designation: Femo TypeStrain=True Application: Characterization Respiratory ... Actinobacillus pleuropneumoniae (Shope) Pohl et al. (ATCC® 33590™) Strain Designations: Femo / Type Strain: yes / Biosafety ... Analysis of Actinobacillus pleuropneumoniae and related organisms by DNA-DNA hybridization and restriction endonuclease ... Biochemical and serological characterization of Haemophilus pleuropneumoniae (Matthews and Pattison 1961) Shope 1964 and ...
The Gram-negative bacterium Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumoniae, a ... Comparative Genomic Characterization of Actinobacillus pleuropneumoniae Zhuofei Xu, Xiabing Chen, Lu Li, Tingting Li, Shengyue ... Comparative Genomic Characterization of Actinobacillus pleuropneumoniae Zhuofei Xu, Xiabing Chen, Lu Li, Tingting Li, Shengyue ... Comparative Genomic Characterization of Actinobacillus pleuropneumoniae Zhuofei Xu, Xiabing Chen, Lu Li, Tingting Li, Shengyue ...
Two Actinobacillus pleuropneumoniae serotype 7 antigens were expressed in Escherichia coli and tested for their protective ... Immunization of pigs against Actinobacillus pleuropneumoniae with two recombinant protein preparations.. Rossi-Campos A1, ... Upon challenge with an A. pleuropneumoniae serotype 7 strain, all immunized groups were less affected by the disease and showed ... Protection was serotype-specific since no cross-protection was detected following challenge with an A. pleuropneumoniae ...
Actinobacillus pleuropneumoniae serovar 10 str. D13039. Actinobacillus pleuropneumoniae serovar 4 str. M62. Actinobacillus ... Actinobacillus pleuropneumoniae serovar 10 str. D13039. Actinobacillus pleuropneumoniae serovar 4 str. M62. Actinobacillus ... Actinobacillus pleuropneumoniae serovar 11 str. 56153. Actinobacillus pleuropneumoniae serovar 13 str. N273. Actinobacillus ... Actinobacillus pleuropneumoniae serovar 11 str. 56153. Actinobacillus pleuropneumoniae serovar 13 str. N273. Actinobacillus ...
... an antigenic membrane protein from Actinobacillus pleuropnemoniae homology to ABC transporters; amino acid sequence in first ... Actinobacillus pleuropneumoniae ApaA protein. Subscribe to New Research on Actinobacillus pleuropneumoniae ApaA protein ... an antigenic membrane protein from Actinobacillus pleuropnemoniae homology to ABC transporters; amino acid sequence in first ...
DNA sequence analysis of the fragment showed that there are three genes designated afuA, afuB and afuC (Actinobacillus ferric ... The cloned afu locus of Actinobacillus pleuropneumoniae restored the ability of an Escherichia coli K-12 mutant (aroB) to grow ... Identification of a locus involved in the utilization of iron by Actinobacillus pleuropneumoniae FEMS Microbiol Lett. 1996 Sep ... The cloned afu locus of Actinobacillus pleuropneumoniae restored the ability of an Escherichia coli K-12 mutant (aroB) to grow ...
... pleuropneumoniae and divided the isolates of A. pleuropneumoniae into 20 clusters. Most of the serovars of A. pleuropneumoniae ... Actinobacillus pleuropneumoniae, p. 563-576. In B. E. Straw, J. J. Zimmerman, S. DAllaire, and D. J. Taylor (ed.), Diseases of ... An Actinobacillus pleuropneumoniae PCR typing system based on the apx and omlA genes-evaluation of isolates from lungs and ... A. pleuropneumoniae is genetically closely affiliated with Actinobacillus lignieresii (6, 25), a bacterium commonly found in ...
The IDEXX APP-ApxIV Ab Test is an enzyme immunoassay for the detection of antibodies against Actinobacillus pleuropneumoniae ( ... Actinobacillus pleuropneumoniae (APP). Swine pleuropneumonia caused by Actinobacillus pleuropneumoniae (APP) is a highly ... Actinobacillus pleuropneumoniae (APP). The IDEXX APP-ApxIV Ab Test is an enzyme immunoassay for the detection of antibodies ... against Actinobacillus pleuropneumoniae (APP), the causative pathogen for swine pleuropneumonia, in serum and plasma of swine ...
In this study, we identified two TolC-like proteins, TolC1 and TolC2, in A. pleuropneumoniae. Deletion of tolC1, but not tolC2 ... In this study, we identified two TolC-like proteins, TolC1 and TolC2, in A. pleuropneumoniae. Deletion of tolC1, but not tolC2 ... pleuropneumoniae. Taken together, TolC1 is required for biofilm formation and is a part of the multidrug resistance machinery ... pleuropneumoniae. Taken together, TolC1 is required for biofilm formation and is a part of the multidrug resistance machinery ...
Actinobacillus pleuropneumoniae and Pasteurella multocida. Pharmacokinetic data were pooled for two bioequivalent products, ... pleuropneumoniae). For daily doses at steady state, and 90% TAR bacteriostatic and bactericidal actions, dosages of 6.2 and 9.6 ... pleuropneumoniae. Using these PK and PD data, together with literature MIC distributions, doses for each pathogen were ... mg/kg (P. multocida) and 18.2 and 35.2 mg/kg (A. pleuropneumoniae) were required. PK/PD integration and modelling approaches to ...
Lipopolysaccharides of Actinobacillus pleuropneumoniae bind pig hemoglobin.. M Bélanger, C Bégin, M Jacques ... Lipopolysaccharides of Actinobacillus pleuropneumoniae bind pig hemoglobin. Message Subject (Your Name) has forwarded a page to ... A previous study indicated that lipopolysaccharides (LPS) extracted from Actinobacillus pleuropneumoniae bind two low-molecular ... Our results indicate that LPS of all A. pleuropneumoniae isolates tested bind pig hemoglobin and that lipid A is involved in ...
MUNOZ, D; QUEZADA, M y RUIZ, A. Serological behaviour study of Actinobacillus pleuropneumoniae (App) in commercial swine herds ... Palabras clave : Actinobacillus pleuropneumoniae; seroprevalence; seroconversion. · resumen en Español · texto en Español · ...
An Actinobacillus pleuropneumoniae (APP) challenge model was used to evaluate the duration of antibiotic effectiveness of ... In the U.S., approval has been granted for treatment of SRD associated with Actinobacillus pleuropneumoniae, Pasteurella ... Draxxin® (tulathromycin) Injectable Solution: Duration of Effectiveness in an Actinobacillus Pleuropneumoniae Challenge Model. ... weight given at different times prior to intranasal challenge with a highly virulent strain of Actinobacillus pleuropneumoniae ...
Keywords: Actinobacillus pleuropneumoniae, enzyme-linked immunosorbent assay, polymerase chain reaction. Cite as: Broes A, ... Serological testing is widely used to monitor swine herds for Actinobacillus pleuropneumoniae (APP). Several serological tests ... Dealing with unexpected Actinobacillus pleuropneumoniae serological results. Andr? Broes, DVM, PhD, Diplomate ECPHM; Guy-Pierre ... Martineau GP, Gottschalk M. Dealing with unexpected Actinobacillus pleuropneumoniae serological results. J Swine Health Prod ...
Actinobacillus pleuropneumoniae infections in pigs: the role of virulence factors in pathogenesis and protection. Vet. ... D) Two wild-type strains of A. pleuropneumoniae and E. coli DH5α expressing the A. pleuropneumoniae pgaCD genes on a plasmid ( ... Expression of A. pleuropneumoniae pgaCD in E. coli.Genomic DNA isolated from A. pleuropneumoniae strain IA1 was amplified by ... Expression and purification of A. pleuropneumoniae dispersin B.Genomic DNA isolated from A. pleuropneumoniae strain IA1 was ...
Actinobacillus pleuropneumoniae (APP) was first isolated from pig lung in 1957 and initially described as Haemophilus-like ... Actinobacillus pleuropneumoniae is one of the most important bacterial pathogens, which causes PCP (Li et al., 2016). For ... 2008). Efficacy of a subunit vaccine against Actinobacillus pleuropneumoniae in an endemically infected swine herd. J. Swine ... 2014). Transmission of Actinobacillus pleuropneumoniae among weaned piglets on endemically infected farms. Prev. Vet. Med. 117 ...
MICs and MBCs of Tiamulin against Actinobacillus pleuropneumoniae (App) in Broth and 100 Per Cent Serum. Disease and welfare ... against Actinobacillus pleuropneumoniae (App), using standardised MIC determinations, have been unsuccessful and led to ... in plasma with tiamulin MICs against Actinobacillus pleuropneumoniae (App) have proven ineffective, despite good clinical and ...
  • Taken together, TolC1 is required for biofilm formation and is a part of the MDR machinery of both planktonic and biofilm cells, which could supplement therapeutic strategies for resistant bacteria and biofilm-related infections of A. pleuropneumoniae clinical isolate SC1516. (frontiersin.org)
  • Actinobacillus is a genus of Gram-negative, nonmotile and nonspore-forming, oval to rod-shaped bacteria occurring as parasites or pathogens in mammals, birds, and reptiles. (wikipedia.org)
  • The present invention pertains to a method to produce RTX-toxin Apxl by culturing Actinobacillus pleuropneumonica bacteria in a liquid culturing medium that supports growth of the bacteria, to which medium a calcium salt is added to form calcium ions in the medium, wherein the culturing medium contains borogluconate to form a calcium borogluconate complex in the medium. (patentsencyclopedia.com)
  • 1. A method to produce RTX-toxin ApxI comprising culturing Actinobacillus pleuropneumoniae bacteria in a culturing medium that supports growth of the bacteria, to which medium a calcium salt is added to form calcium ions in the medium, characterised in that the culturing medium contains borogluconate to form a calcium borogluconate complex in the medium. (patentsencyclopedia.com)
  • Biomarkers of infection were screened for their possible role as evaluators of antibiotic treatment in an aerosol infection model of porcine pneumonia caused by Actinobacillus pleuropneumoniae (Ap). (dtu.dk)
  • The apxIVA gene from the serotype 1 A. pleuropneumoniae type strain Shope 4074T encodes a protein with a predicted molecular mass of 202 kDa which has typical features of RTX proteins including hydrophobic domains in the N-terminal half and 24 glycine-rich nonapeptides in the C-terminal half that bind Ca2+. (nih.gov)
  • Clustering of 26,012 predicted protein-coding genes showed that the pan genome of A. pleuropneumoniae consists of 3,303 gene clusters, which contain 1,709 core genome genes, 822 distributed genes, and 772 strain-specific genes. (asm.org)
  • To evaluate lung lesions at slaughter after three-dose vaccination with a subunit Actinobacillus pleuropneumoniae vaccine containing ApxI, ApxII, ApxIII, and an outer membrane protein. (aasv.org)
  • In reverse transcription PCR differential display (RT-PCR DD) experiments, A. pleuropneumoniae CM5 exposed to BALF up-regulated, among other genes, a gene predicted to encode LamB, an outer-membrane transport protein of the maltose regulon. (biomedcentral.com)
  • Characterization of apxIVA, a new RTX determinant of Actinobacillus pleuropneumoniae. (nih.gov)
  • Amplified fragment length polymorphism (AFLP) was evaluated as a method for genotypic characterization and subtyping within the bacterial species Actinobacillus pleuropneumoniae . (asm.org)
  • Evaluar las lesiones pulmonares en el rastro después de una vacunación con tres dosis de una vacuna de Actinobacillus pleuropneumoniae de subunidades conteniendo ApxI, ApxII, ApxIII, y una proteína de membrana externa. (aasv.org)
  • The goals of this study were to determine the role of A. pleuropneumoniae exotoxin ApxI in cytokine induction and to delineate the underlying mechanisms. (biomedcentral.com)
  • Among various vaccines against Actinobacillus pleuropneumoniae, subunit vaccines using recombinant proteins of ApxI, ApxII, and ApxIII as vaccine antigens have shown good efficacy in terms of safety and protection. (bvsalud.org)
  • Pleuropneumonia is caused by Actinobacillus pleuropneumoniae , a Gram negative rod-shaped bacterium which produces fimbriae (filaments), a protective capsule and at least four toxins (ApxI, ApxII, which are haemolytic and destroy red blood cells, ApxIII which kills cells and Apx IV, produced in vivo and essential for pathogenicity). (pigprogress.net)
  • At slaughter, lungs were examined for gross and microscopic lesions and cultured for A pleuropneumoniae . (aasv.org)
  • Differences in ADG and occurrence of A pleuropneumoniae -related lesions were compared between treatment groups using multilevel mixed models with room and pen as random effects. (aasv.org)
  • Actinobacillus pleuropneumoniae -related lesions and ADG did not differ between control and treatment groups. (aasv.org)
  • A robust antibody titer is essential in preventing lung lesions associated with Actinobacillus pleuropneumoniae (App). (izon.com)
  • Attempts to integrate the pharmacokinetics (PK) of tiamulin concentrations in plasma with the pharmacodynamics (PD) measured as Minimum Inhibitory Concentration (MIC) against Actinobacillus pleuropneumoniae (App), using standardised MIC determinations, have been unsuccessful and led to considerations that lung 1 or leucocyte concentrations 2 might play a significant role, as estimated intracellular concentrations were 8.5µg/g but only 0.47µg/ml in plasma (ratio 18:1). (thepigsite.com)
  • AasP, an autotransporter serine protease of Actinobacillus pleuropneumoniae, has been shown to be expressed in necrotic porcine lung tissue. (uni-bielefeld.de)
  • To date, several factors involved in the virulence of A. pleuropneumoniae have been described, including Apx exotoxins, capsular polysaccharides (CPS), lipopolysaccharides (LPS), outer membrane proteins, iron-acquisition proteins and adhesin factors ( 11 , 19 , 24 ). (asm.org)
  • A previous study indicated that lipopolysaccharides (LPS) extracted from Actinobacillus pleuropneumoniae bind two low-molecular-mass proteins, of approximately 10 and 11 kDa, present in porcine respiratory tract secretions (M. Bélanger, D. Dubreuil, and M. Jacques, Infect. (asm.org)
  • Besides, biofilm formation was inhibited and drug sensitivity was increased by the addition of phenylalanine-arginine beta-naphthylamide (PAβN), a well-known efflux pump inhibitor (EPI), suggesting a role for EPI in antibacterial strategies toward drug tolerance of A. pleuropneumoniae . (frontiersin.org)
  • In A. pleuropneumoniae , biofilm formation is prevalent among field isolates ( Kaplan and Mulks, 2005 ). (frontiersin.org)
  • An isogenic Actinobacillus pleuropneumoniae AasP mutant exhibits altered biofilm formation but retains virulence. (uni-bielefeld.de)
  • Tegetmeyer H, Fricke K, Baltes N. An isogenic Actinobacillus pleuropneumoniae AasP mutant exhibits altered biofilm formation but retains virulence. (uni-bielefeld.de)
  • Absence of TolC Impairs Biofilm Formation in Actinobacillus pleuropneumoniae by Reducing Initial Attachment. (uni-bielefeld.de)
  • Analysis on Actinobacillus pleuropneumoniae LuxS regulated genes reveals pleiotropic roles of LuxS/AI-2 on biofilm formation, adhesion ability and iron metabolism. (uni-bielefeld.de)
  • A total of 430 newborn piglets in a herd endemically infected with A pleuropneumoniae were assigned to control and treatment groups by block randomization at litter level. (aasv.org)
  • Actinobacillus pleuropneumoniae (Shope) Pohl et al. (atcc.org)
  • To investigate the associations of serovar diversity with the underlying genetic components, more serovar-related genomic islands involved in the biosynthesis of capsular and lipopolysaccharide antigens should be decoded at the pan-genome level of A. pleuropneumoniae . (asm.org)
  • O segundo segmento diferencial foi identificado apenas no genoma de A. pleuropneumoniae sorotipo 7 (AP76) e contém sequências como transposases caracterizando a presença de uma sequência de inserção no segmento. (ufv.br)
  • Under controlled conditions, 170 F2 animals of a Hampshire/Landrace family, with known differences in founder populations regarding A. pleuropneumoniae resistance, were challenged with an A. pleuropneumoniae serotype 7 aerosol followed by a detailed clinical, radiographic, ultrasonographic, pathological and bacteriological examination. (deepdyve.com)
  • Waldmann, Karl 2014-01-21 00:00:00 Actinobacillus pleuropneumoniae is among the most important pathogens worldwide in pig production. (deepdyve.com)