Actinobacillus pleuropneumoniae: A species of gram-negative, facultatively anaerobic coccobacillus-shaped bacteria that has been isolated from pneumonic lesions and blood. It produces pneumonia with accompanying fibrinous pleuritis in swine.Actinobacillus Infections: Infections with bacteria of the genus ACTINOBACILLUS.Actinobacillus: A genus of PASTEURELLACEAE described as gram-negative, nonsporeforming, nonmotile, facultative anaerobes. Most members are found both as pathogens and commensal organisms in the respiratory, alimentary, and genital tracts of animals.Pleuropneumonia: Inflammation of the lung parenchyma that is associated with PLEURISY, inflammation of the PLEURA.Swine Diseases: Diseases of domestic swine and of the wild boar of the genus Sus.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Aggregatibacter actinomycetemcomitans: A species of Gram-negative, facultatively anaerobic spherical or rod-shaped bacteria indigenous to dental surfaces. It is associated with PERIODONTITIS; BACTERIAL ENDOCARDITIS; and ACTINOMYCOSIS.Haemophilus: A genus of PASTEURELLACEAE that consists of several species occurring in animals and humans. Its organisms are described as gram-negative, facultatively anaerobic, coccobacillus or rod-shaped, and nonmotile.Actinobacillus suis: A species of gram-negative bacteria in the genus ACTINOBACILLUS. It is mainly a pathogen of PIGS, but also can infect HORSES.Hemolysin Proteins: Proteins from BACTERIA and FUNGI that are soluble enough to be secreted to target ERYTHROCYTES and insert into the membrane to form beta-barrel pores. Biosynthesis may be regulated by HEMOLYSIN FACTORS.Serotyping: Process of determining and distinguishing species of bacteria or viruses based on antigens they share.Transferrin-Binding Proteins: A class of carrier proteins that bind to TRANSFERRIN. Many strains of pathogenic bacteria utilize transferrin-binding proteins to acquire their supply of iron from serum.Cytotoxins: Substances that are toxic to cells; they may be involved in immunity or may be contained in venoms. These are distinguished from CYTOSTATIC AGENTS in degree of effect. Some of them are used as CYTOTOXIC ANTIBIOTICS. The mechanism of action of many of these are as ALKYLATING AGENTS or MITOSIS MODULATORS.Pasteurella multocida: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria normally found in the flora of the mouth and respiratory tract of animals and birds. It causes shipping fever (see PASTEURELLOSIS, PNEUMONIC); HEMORRHAGIC BACTEREMIA; and intestinal disease in animals. In humans, disease usually arises from a wound infection following a bite or scratch from domesticated animals.Pleuropneumonia, Contagious: A pleuropneumonia of cattle and goats caused by species of MYCOPLASMA.Aspartate Ammonia-Lyase: An enzyme that catalyzes the conversion of aspartic acid to ammonia and fumaric acid in plants and some microorganisms. EC 4.3.1.1.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Pasteurellaceae: A family of coccoid to rod-shaped nonsporeforming, gram-negative, nonmotile, facultatively anaerobic bacteria that includes the genera ACTINOBACILLUS; HAEMOPHILUS; MANNHEIMIA; and PASTEURELLA.Bacterial Proteins: Proteins found in any species of bacterium.Transferrin-Binding Protein B: A subtype of bacterial transferrin-binding protein found in bacteria. It forms a cell surface receptor complex with TRANSFERRIN-BINDING PROTEIN A.Bacterial Vaccines: Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.Pasteurella: The oldest recognized genus of the family PASTEURELLACEAE. It consists of several species. Its organisms occur most frequently as coccobacillus or rod-shaped and are gram-negative, nonmotile, facultative anaerobes. Species of this genus are found in both animals and humans.Palatine Tonsil: A round-to-oval mass of lymphoid tissue embedded in the lateral wall of the PHARYNX. There is one on each side of the oropharynx in the fauces between the anterior and posterior pillars of the SOFT PALATE.Tylosin: Macrolide antibiotic obtained from cultures of Streptomyces fradiae. The drug is effective against many microorganisms in animals but not in humans.Actinobacillus equuli: A genus of gram-negative bacteria in the genus ACTINOBACILLUS, which is pathogenic for HORSES and PIGS.Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Genes, Bacterial: The functional hereditary units of BACTERIA.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Bacterial Capsules: An envelope of loose gel surrounding a bacterial cell which is associated with the virulence of pathogenic bacteria. Some capsules have a well-defined border, whereas others form a slime layer that trails off into the medium. Most capsules consist of relatively simple polysaccharides but there are some bacteria whose capsules are made of polypeptides.Iron-Binding Proteins: Proteins that specifically bind to IRON.Bacterial Toxins: Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.Lung: Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.Bacterial Outer Membrane Proteins: Proteins isolated from the outer membrane of Gram-negative bacteria.Sulfite Reductase (Ferredoxin): A FERREDOXIN-dependent oxidoreductase that is primarily found in PLANTS where it plays an important role in the assimilation of SULFUR atoms for the production of CYSTEINE and METHIONINE.Polysaccharides, Bacterial: Polysaccharides found in bacteria and in capsules thereof.Haemophilus paragallinarum: A species of gram-negative bacteria in the genus HAEMOPHILUS, causing respiratory tract disease in CHICKENS known as infectious coryza.Pasteurella Infections: Infections with bacteria of the genus PASTEURELLA.Mannheimia haemolytica: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria normally commensal in the flora of CATTLE and SHEEP. But under conditions of physical or PHYSIOLOGICAL STRESS, it can cause MASTITIS in sheep and SHIPPING FEVER or ENZOOTIC CALF PNEUMONIA in cattle. Its former name was Pasteurella haemolytica.
(1/288) Pulmonary lesions in guinea pigs experimentally infected with Actinobacillus pleuropneumoniae (A.p.) serovar 1.

Pathological studies were carried out on the lungs of guinea pigs intratracheally inoculated with 4.6 x 10(6-8) colony forming units (CFU)/head of Actinobacillus pleuropneumoniae serovar 1. All animals in the highest dose group died within 24 hr post inoculation (hpi) and showed pulmonary lesions being hemorrhagic in nature while all animals in the lowest dose group were killed as scheduled at 11 days post inoculation (dpi) and showed only hyperplasia of peribronchial lymphoid tissues. In the middle dose group, two died within 24 hpi, two died at 9 dpi, and the remaining one was killed at 11 dpi. Two guinea pigs which died at 9 dpi showed fibrinonecrotic pleuropneumonia which is the most characteristic acute pulmonary lesion in swine, and has not yet been reproduced in laboratory animals up to the present time. This suggests that guinea pigs may be a useful laboratory animal for studying the pathogenesis of Actinobacillus pleuropneumoniae infection in swine.  (+info)

(2/288) Vaccination and protection of pigs against pleuropneumonia with a vaccine strain of Actinobacillus pleuropneumoniae produced by site-specific mutagenesis of the ApxII operon.

The production of toxin (Apx)-neutralizing antibodies during infection plays a major role in the induction of protective immunity to Actinobacillus pleuropneumoniae reinfection. In the present study, the gene encoding the ApxII-activating protein, apxIIC, was insertionally inactivated on the chromosome of a serovar 7 strain, HS93. Expression of the structural toxin, ApxIIA, and of the two genes required for its secretion, apxIB and apxID, still occurs in this strain. The resulting mutant strain, HS93C- Ampr, was found to secrete the unactivated toxin. Pigs vaccinated with live HS93C- Ampr via the intranasal route were protected against a cross-serovar challenge with a virulent serovar 1 strain of A. pleuropneumoniae. This is the first reported vaccine strain of A. pleuropneumoniae which can be delivered live to pigs and offers cross-serovar protection against porcine pleuropneumonia.  (+info)

(3/288) Molecular cloning and sequencing of the aroA gene from Actinobacillus pleuropneumoniae and its use in a PCR assay for rapid identification.

The gene (aroA) of Actinobacillus pleuropneumoniae, serotype 2, encoding 5-enolpyruvylshikimate-3-phosphate synthase was cloned by complementation of the aroA mutation in Escherichia coli K-12 strain AB2829, and the nucleotide sequence was determined. A pair of primers from the 5' and 3' termini were selected to be the basis for development of a specific PCR assay. A DNA fragment of 1,025 bp was amplified from lysed A. pleuropneumoniae serotypes 1 to 12 of biovar 1 or from isolated DNA. No PCR products were detected when chromosomal DNAs from other genera were used as target DNAs; however, a 1,025-bp DNA fragment was amplified when Actinobacillus equuli chromosomal DNA was used as a target, which could be easily differentiated by its NAD independence. The PCR assay developed was very sensitive, with lower detection limits of 12 CFU with A. pleuropneumoniae cells and 0.8 pg with extracted DNA. Specificity and sensitivity make this PCR assay a useful method for the rapid identification and diagnosis of A. pleuropneumoniae infections.  (+info)

(4/288) Actinobacillus pleuropneumoniae osteomyelitis in pigs demonstrated by fluorescent in situ hybridization.

Necrotizing osteomyelitis and fibrinopurulent arthritis with isolation of Actinobacillus pleuropneumoniae serotype 2 is reported in two pigs from a herd with lameness and mild coughing problems among 8 to 12-week-old pigs. Application of fluorescent in situ hybridization targeting 16S ribosomal RNA of A. pleuropneumoniae in formalin-fixed tissue was performed to verify the association of A. pleuropneumoniae with the bone and joint lesions. By in situ hybridization A. pleuropneumoniae was demonstrated as multiple microcolonies or single cells dispersed in focal fibrinonecrotizing pleuropneumonia, in joints with arthritis, and in bone necroses including lysis of growth plate and suppurative inflammation in the adjacent trabecular metaphysis, thus demonstrating that well-known infections manifest new, unusual lesions.  (+info)

(5/288) A novel enzyme-linked immunosorbent assay using the recombinant Actinobacillus pleuropneumoniae ApxII antigen for diagnosis of pleuropneumonia in pig herds.

For the surveillance of pig herds infected with porcine pleuropneumonia, an enzyme-linked immunosorbent assay (ELISA) using the recombinant Actinobacillus pleuropneumoniae ApxII protein as species- but not serotype-specific antigen was developed. Using this ELISA, 243 of 400 animals from 22 A. pleuropneumoniae-infected herds were classified as seropositive.  (+info)

(6/288) First chromosomal restriction map of Actinobacillus pleuropneumoniae and localization of putative virulence-associated genes.

Combined physical and genetic maps of the genomes of Actinobacillus pleuropneumoniae AP76 (serotype 7 clinical isolate) and of A. pleuropneumoniae ATCC 27088 (serotype 1 reference strain) were constructed by using the restriction endonucleases ApaI, AscI, NotI, and SalI. The chromosome sizes as determined by the addition of estimated fragment sizes were 2.4 Mbp, and both maps had a resolution of approximately 100 kbp. The linkages between the ApaI, AscI, NotI, and SalI fragments and their relative positions were determined by (i) fragment excision and redigestion and (ii) partial digests of defined fragments and Southern blot using end-standing probes. The single SalI site within the chromosome of strain A. pleuropneumoniae AP76 was defined as position 1 of the map; for the map of A. pleuropneumoniae ATCC 27088, the corresponding SalI site was chosen. Putative virulence-associated genes (apx, omlA, sodA, tbpBA, ureC, and a repeat element) and housekeeping genes (glyA, metJ, recA, and rhoAP) were positioned on the physical maps and located on the ApaI and NotI fragments of A. pleuropneumoniae serotype reference strains.  (+info)

(7/288) Detection of antibodies against Actinobacillus pleuropneumoniae serotypes 1, 2, 5 and 7 using the immunohistochemical staining.

Whole cells of Actinobacillus pleuropneumoniae (A. pleuropneumoniae) serotype 1, 2, 5 or 7 attached to fibrins were fixed in 10% neutral buffered formalin and embedded in paraffin. The sections on a slide glass were stained by the avidin-biotin complex immunoperoxidase (ABC) method. Test sera were applied to sections as primary antibodies. The serum antibodies against A.pleuropneumoniae (serotypes 1, 2, 5 and 7) were measured by the ABC method and complement fixation (CF) test. There was good correlation between the ABC and CF tests. The present results indicate that the immunohistochemical staining is as useful as the CF test for the detection and quantification of antibody in swine sera.  (+info)

(8/288) Characterization of apxIVA, a new RTX determinant of Actinobacillus pleuropneumoniae.

A fourth type of RTX determinant was identified in Actinobacillus pleuropneumoniae and was designated apxIVA. When expressed in Escherichia coli, recombinant ApxIVA showed a weak haemolytic activity and co-haemolytic synergy with the sphingomyelinase (beta-toxin) of Staphylococcus aureus. These activities required the presence of an additional gene, ORF1, that is located immediately upstream of apxIVA. The apxIVA gene product could not be detected in A. pleuropneumoniae cultures grown under various conditions in vitro; however, pigs experimentally infected with A. pleuropneumoniae serotypes 1, 5 and 7 started to produce antibodies that reacted with recombinant ApxIVA 14 d post-infection, indicating that apxIVA is expressed in vivo. In addition, sera from pigs naturally and experimentally infected with any of the serotypes all reacted with recombinant ApxIVA. The apxIVA gene from the serotype 1 A. pleuropneumoniae type strain Shope 4074T encodes a protein with a predicted molecular mass of 202 kDa which has typical features of RTX proteins including hydrophobic domains in the N-terminal half and 24 glycine-rich nonapeptides in the C-terminal half that bind Ca2+. The glycine-rich nonapeptides are arranged in a modular structure and there is some variability in the number of modules in the ApxIVA proteins of different serotypes of A. pleuropneumoniae. The deduced amino acid sequences of the ApxIVA proteins have significant similarity with the Neisseria meningitidis iron-regulated RTX proteins FrpA and FrpC, and to a much lesser extent with other RTX proteins. The apxIVA gene could be detected in all A. pleuropneumoniae serotypes and seems to be species-specific. Although the precise role of this new RTX determinant in pathogenesis of porcine pleuropneumonia needs to be determined, apxIVA is the first in vivo induced toxin gene that has been described in A. pleuropneumoniae.  (+info)

*  Actinobacillus pleuropneumoniae
... (previously Haemophilus pleuropneumoniae), is a Gram-negative, facultative anaerobic, ... Gillaspy, A. "Actinobacillus pleuropneumoniae". Laboratory for Molecular Biology and Cytometry Research-University of Oklahoma ... "Actinobacillus pleuropneumoniae". Iowa State University College of Veterinary Medicine. Retrieved 20 April 2013. [permanent ... Brownfield, B. "Actinobacillus pleuropneumoniae in swine". Purdue University-Animal Disease Diagnostic Laboratory. Retrieved 20 ...
*  Pasteurellaceae
... while the other includes Actinobacillus minor, Actinobacillus pleuropneumoniae, Haemophilus ducreyi, Haemophilus parasuis, and ... The distribution of CSIs corresponds to sensu stricto clades of "true" Actinobacillus, Haemophilus, and Pasteurella species, ... and Actinobacillus species. Comparative analyses of Pasteurellaceae genomes have identified large numbers (>20) of conserved ... Actinobacillus, Haemophilus, and Pasteurella. These genera demonstrate extensive polyphyly across the family, however, CSIs ...
*  Actinobacillus
2008). "Actinobacillus pleuropneumoniae". Pasteurellaceae: Biology, Genomics and Molecular Aspects. Caister Academic Press. ... Actinobacillus (Pasteurella) ureae and A. hominis occur in the respiratory tracts of healthy humans and may be involved in the ... Actinobacillus is a genus of Gram-negative, nonmotile and nonspore-forming, oval to rod-shaped bacteria occurring as parasites ... ISBN 978-1-904455-24-0. Nørskov-Lauritsen N, Kilian M (September 2006). "Reclassification of Actinobacillus ...
*  Vaccine
"Development of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae infection". Vaccine. 24 (49): 7226-32. doi: ... Actinobacillus pleuropneumonia and Salmonella infections in pigs. Vaccine development has several trends: Until recently,[when ...
*  Dispersin B
"Poly-N-acetylglucosamine mediates biofilm formation and antibiotic resistance in Actinobacillus pleuropneumoniae". Microb ... Kaplan JB, Ragunath C, Ramasubbu N, Fine DH (August 2003). "Detachment of Actinobacillus actinomycetemcomitans biofilm cells by ... Kaplan JB, Meyenhofer MF, Fine DH (2003). "Biofilm growth and detachment of Actinobacillus actinomycetemcomitans". J. Bacteriol ... a biofilm-releasing glycoside hydrolase from the periodontopathogen Actinobacillus actinomycetemcomitans". J. Mol. Biol. 349 (3 ...
*  Sulfatide
Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae are pathogens that cause respiratory disease in swine. Haemophilus ... coli TOP10 strain Campylobacter jejuni Mycoplasma hyopneumoniae Haemophilus influenzae Actinobacillus pleuropneumoniae ...
*  Actinobacillus suis
Streptococcus suis and Actinobacillus pleuropneumoniae. Antibiotics such as ceftiofur, gentamicin and trimethoprim/sulfadiazine ... Actinobacillus suis is a beta-haemolytic, Gram-negative bacterium of the Pasteurellaceae family. The bacterium has many strains ... Type strain of Actinobacillus suis at BacDive - the Bacterial Diversity Metadatabase. ... Actinobacillus suis, reviewed and published by Wikivet at http://en.wikivet.net/Actinobacillus_suis accessed 07/10/2011. ...
*  Conserved signature indels
Clade II, encompassing Actinobacillus pleuropneumoniae, Actinobacillus minor, Haemophilus ducreyi, Mannheimia haemolytica and ... Two major clades are formed within this Pasteurellales: Clade I, encompassing Aggregatibacter, Pasteurella, Actinobacillus ...
*  Cooperative Research Centre
... reducing pig deaths and disease from the bacteria Actinobacillus pleuropneumoniae. The Molecular Plant Breeding CRC developed ...
*  Cefquinome
Actinobacillus pleuropneumoniae, and Streptococcus suis. Mastitis-metritis-agalactia syndrome involved with E. coli, ...
*  List of MeSH codes (B03)
Actinobacillus equuli MeSH B03.440.450.009.580 --- Actinobacillus pleuropneumoniae MeSH B03.440.450.009.675 --- Actinobacillus ... Actinobacillus equuli MeSH B03.660.250.550.050.580 --- Actinobacillus pleuropneumoniae MeSH B03.660.250.550.050.675 --- ... Actinobacillus seminis MeSH B03.660.250.550.050.700 --- Actinobacillus suis MeSH B03.660.250.550.290 --- Haemophilus MeSH ... Actinobacillus MeSH B03.660.250.550.050.050 --- Actinobacillus actinomycetemcomitans MeSH B03.660.250.550.050.200 --- ...
In Denmark porcine pleuropneumonia is most frequently caused by Actinobacillus pleuropneumoniae serotype 2 (60%). Isolation of A. pleuropneumoniae from nasal cavities or tonsils from carrier animals is complicated due to the mixed bacterial flora present. An immunomagnetic separation technique (IMS) using immunomagnetic beads (Dynabeads(R)) was developed for isolation of A. pleuropneumoniae serotype 2 from pure cultures and from heterogeneous suspensions. Different coating and washing procedures were evaluated in pure and mixed cultures using polyclonal (PAb) and monoclonal antibodies. The highest reisolation yield was achieved when the beads were coated with 1.5 mug PAb IgG/10(7) beads. After washing the beads for four times 9-24% of the bacteria could be reisolated depending on the amount of IgG attached to the beads and the number of beads used. The recovery was increased to 19-61% when only two washing steps were performed. The IMS was further evaluated using dilutions of A. pleuropneumoniae ...
Serotypes 3 and 8 of Actinobacillus pleuropneumoniae, the aetiological agent of porcine pleuropneumonia, have been reported to predominate in the UK. Direct serotyping of isolates of the organism is typically determined by the immunological reactivity of rabbit serum to its surface polysaccharides, but the method has limitations, for example, cross-reactions between serotypes 3, 6 and 8. This study describes the development of a serotype 3-specific PCR, based on the capsule locus, which can be used in a multiplex format with the organisms specific gene apxIV. The PCR test was evaluated on 266 strains of A pleuropneumoniae and 121 strains of other organisms, including all the major respiratory bacterial pathogens of pigs. The test was highly specific and sensitive and should be useful for differentiating strains of serotypes 3, 6 and 8, and in seroprevalence and epidemiological surveys in regions where serotype 3 is prevalent, such as the UK.. ...
article{176028, author = {Haesebrouck, Freddy and Chiers, Koen and Van Overbeke, Ingrid and Ducatelle, Richard}, issn = {0378-1135}, journal = {VETERINARY MICROBIOLOGY}, language = {eng}, number = {2-4}, pages = {239--249}, title = {Actinobacillus pleuropneumoniae infections in pigs: the role of virulence factors in pathogenesis and protection.}, volume = {58}, year = {1997 ...
Actinobacillus pleuropneumoniae (previously Haemophilus pleuropneumoniae), is a Gram-negative, facultative anaerobic, respiratory pathogen found in pigs. It was first reported in 1957, and was formally declared to be the causative agent of porcine pleuropneumonia in 1964. It was reclassified in 1983 after DNA studies showed it was more closely related to A. lignieresii. A. pleuropneumoniae is a nonmotile, Gram-negative, encapsulated coccobacillus bacterium found in the Pasteurellaceae family. It exhibits β-hemolysis activity, thus explaining its growth on chocolate or blood agar, but must be supplemented with NAD (V factor) to facilitate growth for one of its biological variants (biovar 1). As a facultative anaerobic pathogen, A. pleuropneumoniae may need CO2 to grow. Depending on the biovar, the bacteria may or may not be positive for urease; both biovars are positive for porphyrin. A. pleuropneumoniae was found to be the causative agent for up to 20% of all bacterial pneumonia cases in ...
Fifty randomly selected fattening pig herds were studied to investigate the epidemiological characteristics of infections with Actinobacillus pleuropneumoniae serovars 2, 3 and 9, and to identify risk factors for their within-herd seroprevalences. Information about 13 farm characteristics was obtained by means of a questionnaire and used to assess potential risk factors for the percentage of slaughter pigs with antibodies against each of the three serovars. The presence of antibodies was measured with an indirect ELISA. The median within-herd seroprevalence for serovar 2 was 58 per cent (range 0 to 100 per cent), for serovar 3, 53 per cent (range 10 to 95 per cent), and for serovar 9, 35 per cent (range 5 to 100 per cent). All but one farm tested positive for A pleuropneumoniae serovar 2, and all the farms were positive for A pleuropneumoniae serovars 3 and 9. There was a positive association (P<0.05) between each pair of serovars. The within-herd seroprevalence of serovar 2 was ...
Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, a significant disease that causes serious economic losses to the swine industry worldwide. Persistent infections caused by bacterial biofilms are recalcitrant to treat because of the particular drug resistance of biofilm-dwelling cells. TolC, a key component of multidrug efflux pumps, are responsible for multidrug resistance in many Gram-negative bacteria. In this study, we identified two TolC-like proteins, TolC1 and TolC2, in A. pleuropneumoniae. Deletion of tolC1, but not tolC2, caused a significant reduction in biofilm formation, as well as increased drug sensitivity of both planktonic and biofilm cells. The genetic-complementation of the tolC1 mutation restored the competent biofilm and drug resistance. Besides, biofilm formation was inhibited and drug sensitivity was increased by the addition of phenylalanine-arginine beta-naphthylamide (PAβN), a well-known efflux pump inhibitor (EPI), suggesting a role
0004] It is commonly known how to produce the RTX-toxin ApxI by culturing Actinobacillus pleuropneumoniae in a culturing medium to which a calcium salt (i.e. a chemical compound, based on an acid, formed by replacing all or part of the hydrogen ions of the acid with one or more calcium ions) is added. In particular, EP 0 453 024 already describes such a method (see "Example 2", paragraph 2 "Purification and characterisation of hemolysin", subparagraph "Methods"). Note that ApxI used to be referred to as "HLY" (see Frey et al. in "J Gen Microbiol. 1993 August; 139(8): 1723-8"). From this EP patent it is known to add a calcium compound (CaCl2) to the medium. Indeed, in Microbiol Pathogenesis 37 (2004) 29-33 it is stated that transcriptional activity of the ApxI operon is enhanced by addition of calcium to the growth medium. This way, high levels of ApxI can be provided for. The medium should support the growth of APP bacteria. It is commonly known how to constitute a medium that supports growth of ...
Actinobacillus pleuropneumoniae ApaA protein: an antigenic membrane protein from Actinobacillus pleuropnemoniae homology to ABC transporters; amino acid sequence in first source
Pleuropneumonia infectioasa a porcului (PIP), cauzata de Actinobacillus pleuropneumoniae (App), poate fi intalnita la porcii de toate varstele, inclusiv la mistreti, dar simptomele clinice cele mai frecvente sunt inregistrate la porcii in faza de finisare, in varsta de peste 12 saptamani. In formele clasice de boala animalele manifesta febra si simptome respiratorii, datorate leziunilor caracteristice de pneumonie hemoragico-necrotica sau pleuropneumonie fibrinoasa. Boala poate evolua supra-acut, fara semne clinice, animalele fiind gasite moarte, acut, mai ales in efectivele naive imunologic, cu pierderi prin mortalitate de 15-20%, sau sub-acut, cu mortalitate mai redusa dar variabila. In efectivele infectate endemic boala se cronicizeaza la animalele care au supravietuit, afectand starea lor de bine si producand o scadere a performantelor zootehnice. Porcii pot fi purtatori fara sa dezvolte vreun raspuns imun. Boala genereaza pierderi economice insemnate.. ...
LIU, Qiong et al. Construction and immunogenicity of a ΔapxIC/ompP2 mutant of Actinobacillus pleuropneumonias and Haemophilus parasuis. Onderstepoort j. vet. res. [online]. 2013, vol.80, n.1, pp.1-6. ISSN 2219-0635.. The apxIC genes of the Actinobacillus pleuropneumoniae serovar 5 (SC-1), encoding the ApxI-activating proteins, was deleted by a method involving sucrose counter-selection. In this study, a mutant strain of A. pleuropneumoniae (SC-1) was constructed and named AapxIC/ ompP2. The mutant strain contained foreign DNA in the deletion site of ompP2 gene of Haemophilus parasuis. It showed no haemolytic activity and lower virulence of cytotoxicity in mice compared with the parent strain, and its safety and immunogenicity were also evaluated in mice. The LD50 data shown that the mutant strain was attenuated 30-fold, compared with the parent strain (LD50 of the mutant strain and parent strain in mice were determined to be 1.0 x 107 CFU and 3.5 x 105 CFU respectively). The mutant strain that ...
Structural studies of the O-chain of the phenol-phase soluble lipopolysaccharide from Haemophilus pleuropneumoniae serotype 2: Biochem.Cell Biol.
Looking for online definition of Actinobacillus actinomycetemcomitans in the Medical Dictionary? Actinobacillus actinomycetemcomitans explanation free. What is Actinobacillus actinomycetemcomitans? Meaning of Actinobacillus actinomycetemcomitans medical term. What does Actinobacillus actinomycetemcomitans mean?
The complete nucleotide sequence of a 7.7kb mobilisable plasmid (pM3446F), isolated from a florfenicol resistant isolate of Actinobacillus pleuropneumoniae, showed extended similarity to plasmids found in other members of the Pasteurellaceae containing the floR gene as well as replication and mobilisation genes. Mobilisation into other Pasteurellaceae species confirmed that this plasmid can be transferred horizontally ...
AccuPower® Porcine Respiratory 4-Plex PCR Kit simultaneously detects four pathogens of Actinobacillus pleuropneumoniae (App), Actinobacillus suis (A. suis), Haemophilus parasuis (H. Parasuis) and Streptococcus suis (S. Suis) that cause a porcine respiratory disease ...
The domestic pig (Sus scrofa domestica) has assumed a significant role in biomedical research. The pig serves as an animal model for research involving the dermal, cardiopulmonary, gastrointestinal, renal, and other body systems. Swine have become the organ donor of choice in xenotransplantation experiments. In this regard, porcine reproductive and respiratory syndrome virus (PRRSV) is often found as a copathogen, along with Mycoplasma hyopneumoniae, Pasteurella multocida, porcine circovirus type 2, swine influenza virus, and other pathogens. Swine influenza virus (SIV) is a single-stranded RNA (ssRNA) virus in the family Orthomyxoviridae. SIV is one of the most studied of swine viral pathogens. More severe clinical signs accompany concurrent infection with pathogens such as Actinobacillus pleuropneumoniae, Pasteurella multocida, Haemophilus parasuis, Streptococcus suis type 2, Mycoplasma hyopneumoniae, and PRRSV. Clinical disease may accompany primary viral infection, such as swine influenza virus,
Oral fluid analysis for herd monitoring is of interest to the commercial pig production in Korea. The aim of this study was to investigate pathogen-positive rates and correlations among eight pathogens associated with porcine respiratory disease complex by analyzing oral fluid samples from 214 pig groups from 56 commercial farms. Samples collected by a rope-chewing method underwent reverse-transcriptase polymerase chain reaction (RT-PCR) or standard polymerase chain reaction (PCR) analysis, depending on the microorganism. Pathogens were divided into virus and bacteria groups. The former consisted of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 (PCV2), and the latter Pasteurella multocida, Haemophilus parasuis, Actinobacillus pleuropneumoniae, Mycoplasma hyopneumoniae (MHP), Mycoplasma hyorhinis, and Streptococcus suis (SS). All pathogens were detected more than once by PCR. Age-based analysis showed the PCR-positive rate increased with increasing age for PCV2 ...
Staphylococcus spp. Streptococcus spp. E. coli Pasteurella multocida *Amoxicillin-clavulanate breakpoints were determined from an examination of MIC distribution of isolates, efficacy data, and PK-PD analysis of amoxicillin in dogs. The dosage regimen used for PK-PD analysis of amoxicillin was 11 mg/kg administered every 12 hours orally. **Amoxicillin-clavulanate breakpoints were determined from an examination of MIC distribution of isolates, efficacy data, and PK-PD analysis of amoxicillin in cats at a dosage of 12.5 mg/kg (amoxicillin) administered every 12 hours orally. Ampicillin Actinobacillus pleuropneumoniae Pasteurella multocida Streptococcus suis Bordetella bronchiseptica * Systemic breakpoint derived from microbiological, pharmacokinetic, and pharmacodynamic data. For dogs, the dose of amoxicillin modeled was 22 mg/kg every 12 hours orally. ** A breakpoint of ≤8 should be used for urinary tract infections. This breakpoint was derived from published literature in which orally ...
Actinobacillus definition, any of several spherical or rod-shaped, aerobic, parasitic bacteria of the genus Actinobacillus, certain species of which, as A. mallei, are pathogenic for animals. See more.
Looking for contagious caprine/ovine pleuropneumonia? Find out information about contagious caprine/ovine pleuropneumonia. 1. capable of being passed on by direct contact with a diseased individual or by handling clothing, etc., contaminated with the causative agent 2.... Explanation of contagious caprine/ovine pleuropneumonia
A little over two years ago, we commented on a general rise in the frequency of Actinobacillus suis (A. suis) isolation from our swine tissue submission cases, and wondered if it was related to the increased porcine circovirus type 2 (PCV2) activity (National Hog Farmer North American Preview, June 6, 2008).
Pathogenic bacteria of the genus Neisseria have a siderophore-independent iron-uptake system reliant on a direct interaction between the bacterial cell and human transferrin (hTf), a serum protein. In the meningococcus, this uptake system is dependent on two surface-exposed, transferrin-binding proteins (Tbps), TbpA and TbpB. TbpA is highly conserved among meningococcal strains, and is thought to be a porin-like integral protein that functions as a gated channel for the passage of iron into the periplasm. TbpB is more variable in size, lipidated and fully surface-exposed. Given its location on the cell surface, its role in pathogenicity and interstrain sequence conservation, TbpA is currently being regarded for inclusion in a meningococcal vaccine effective against all serogroups. This requires gaining knowledge of the ligand-receptor interactions. In the present study we have optimized a procedure for obtaining purified, functionally active recombinant TbpA at a level and stability necessary ...
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Actinobacillus hominis.
Homologous recombination (HR) is essential for the accurate repair of DNA double-strand breaks (DSBs), potentially lethal lesions. HR takes place in the late S-G2 phase of the cell cycle and involves the generation of a single-stranded region of DNA, followed by strand invasion, formation of a Holliday junction, DNA synthesis using the intact strand as a template, branch migration and resolution. It is investigated that RecA/Rad51 family proteins play a central role. The breast cancer susceptibility protein Brca2 and the RecQ helicase BLM (Bloom syndrome mutated) are tumor suppressors that maintain genome integrity, at least in part, through HR ...
This study was carried out in Njiru and Dagorreti slaughterhouses with the objective of comparing the results of complement fixation test (CFT) and pathological lesions in lung samples taken for culture and sero-prevalence of Contagious Bovine Pleuropneumonia (CBPP) among cattle brought for slaughter. A total of 804 serum samples and 40 suspected lung tissues were collected from both abattoirs for laboratory diagnosis. The serum and lung tissues were collected from adult cattle that were brought for slaughter. 416 serum samples were taken from Njiru and the other 388 from Dagoretti slaughterhouse.The serum samples were subjected to the complement fixation test and the lung tissues cultured in both solid media and broth. Growth of the microorganisms in the media was checked once a week for a period of one month. Any media that had no growth after a period of one month was recorded as negative. Animals for slaughter originated from 22 districts and the majority were from the arid and semi-arid ...
Contagious bovine pleuropneumonia (CBPP) is a severe infectious disease caused by Mycoplasma mycoides subsp. mycoides small colony type (M. mycoides SC) and is a vast problem in Africa. Current CBPP prevention is based on attenuated live strain vaccines, but these are limited by factors such as short-term immunity, cold-chain dependence and retained virulence. CBPP can be diagnosed using post-mortem examination, identification of the agent using culture and PCR based methods as well as serological diagnostic methods, but the latter are generally not sensitive enough and there is also demand for an inexpensive, pen side field test.The research presented in this thesis was focused on using recombinantly expressed surface proteins from M. mycoides SC to characterize humoral immune responses to CBPP. Thereby candidate proteins to be used in development of serological diagnostic methods and possibly subunit vaccines could be identified. As a first step, five putative variable surface proteins of M. ...
Abstract Contagious bovine pleuropneumonia (CBPP) is an economically important disease in most of sub-Saharan Africa. A conjoint analysis and ordered probit regression models were used to measure the preferences of farmers for CBPP vaccine and vaccination attributes. This was with regard to inclusion or not of an indicator in the vaccine, vaccine safety, vaccine stability as well as frequency of vaccination, vaccine administration and the nature of vaccination. The analysis was carried out in 190 households in Narok District of Kenya between October and December 2006 using structured questionnaires, 16 attribute profiles and a five-point Likert scale. The factors affecting attribute valuation were shown through a two-way location interaction model. The study also demonstrated the relative importance (RI) of attributes and the compensation value of attribute levels. The attribute coefficient estimates showed that farmers prefer a vaccine that has an indicator, is 100% safe and is administered by ...
The use of the porcine lung to demonstrate recruitment maneuvers stimulated us to consider the possibility of using the ASL 5000 to investigate ways to improve protocols for the handling of ex-vivo perfused lungs. We believe this would provide interesting research projects for students and faculty. Many of the current ex-vivo perfusion protocols involve using positive pressure and high concentrations of oxygen to maintain isolated perfused lungs. The longest time that perfused lungs have been kept viable ex-vivo is about 10 hours. Using the ASL 5000 to model breathing, we would like to explore the possibility of extending survival time of ex-vivo perfused lungs. We believe that lowering the FiO2 and avoiding positive pressure ventilation could improve viability. Further development of this model could lead to advances in therapeutics related to airway clearance, mechanical ventilation, and airway pharmacology.. In the video below, the porcine lungs are attached to an Avea ventilator in the CPAP ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Fast Shipping. Great Low Price. Protects against Haemophilus parasuis, the cause of Glassers disease and polyserositis. Also protects against Actinobacillus pleuropneumonia (serotypes 1, 5, and 7) and Pasteurella multocida. 2 ml dose IM to pigs over 30 lbs. Revaccinate 2-3 weeks later.Parapleuro Shield-P Swine Vaccine Novartis Animal Health Respiratory | Swine Vaccines | Farm
The assumption that USS arise by mutation rather than copying was first confirmed using alignments of H. influenzae proteins with distant homologues. Abundant USS-like repeats were found in all eight Pasteurellacean genomes; the repeat consensuses of species in the Hin subclade were identical to that of H. influenzae (AAGTGCGGT), whereas members of the Apl subclade shared the consensus ACAAGCGGT. All species USSs had the strong consensus and flanking AT-rich repeats of H. influenzae USSs. DNA uptake and competition experiments demonstrated that the Apl-type repeat is a true USS distinct from the Hin-type USS: A. pleuropneumoniae preferentially takes up DNA fragments containing the Apl-type USS over both H. influenzae and unrelated DNAs, and H. influenzae prefers its own USS over the Apl type ...
Succinic Acid (SA) was continuously produced using glucose and a Mg2CO3(OH)2 slurry as feed. Glucose feed concentrations of 20 and 40 g ℓ-1 were employed with corresponding Mg2CO3(OH)2 slurry concentrations of 60 and 120 g ℓ-1. The reactor pH was passively maintained between 6,4 and 6,8 by the buffer properties of the slurry in conjunction with the pH adjusted glucose feed. The suspended cell (SC) reactor was operated at 37°C with dilution rates varying between 0,04 h-1and 0,6 h-1. Groperl® particles were used as inert supports in the biofilm reactor; dilution rates of 0,11 h-1 to 1 h-1 were investigated. Two SC fermentations were conducted for the 20 g ℓ-1 glucose feed concentration and one for the 40 g ℓ-1. All SC fermentation runs were operated in excess of 12 days, while the biofilm run lasted 6,5 days. Fermentations were terminated only after contamination by lactic acid bacteria was observed. SC fermentations with the glucose feed concentration of 20 g ℓ-1 achieved a maximum SA ...
SUMMARY. A case of bilateral epididymitis in a young Friesland bull is described. Actino-bacillis seminis was isolated from the bulls semen. The organism proved to be identical in microscopical, cultural and biochemical characteristics to strains isolated from rams. Serum from this animal, when subjected to a complement fixation test, yielded a suspicious reaction against an antigen prepared from the isolate of a naturally infected ram with clinical epididymitis. Blood sera from two bulls in the Orange Free State gave positive and suspicious reactions respectively to the same antigen.. ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
A genus of very small, nonmotile, nonsporeforming, aerobic, facultatively anaerobic bacteria containing Gram negative rods interspersed with coccal elements. The metabolism of these bacteria is fermentative. They are pathogenic to animals. The…
Rights: This volume was digitized and made accessible online due to deterioration of the original print copy. If you are the author of this work and would like to have online access removed, please contact the Library Administration Office, 785-532-7400, [email protected] ...
Welcome to the Inform a Colleague area of the Academy Website. The American Academy of Spine Physicians (AASP) strives to implement new and more efficient methods for members to communicate with other healthcare professionals. One of the most effective tools is the online "Inform a Colleague" function. This function can be used to expand and educate your spinecare referral network. It also represents a resource to conveniently inform others about trends in spinecare and about offers of organizations such as the American Academy of Spine Physicians (AASP), the International Spine Association (ISA). The link library also contains an application for membership with the AASP ...
Infection may be inapparent, but 15-30% of pigs may suffer depression, inappetence, high fever (41.5°C, 107°F), or laboured breathing, especially after rising to drink of after disturbance within hours of infection. Cyanosis (a purple tinge of the ears and feet), subnormal rectal temperature and death may follow within 4-6 hours of the onset of clinical signs.. Blood stained froth may be seen at the mouth and such pigs will die. Mortality may reach 30-50% of affected pigs. Less severely affected pigs do not eat, are fevered, and show respiratory distress, coughing and exercise intolerance and do not pull away strongly when restrained. Some of these pigs may also die. Recovering animals cough, and show respiratory distress, particularly when disturbed. Exercise intolerance may continue for days and affected animals may eat less, appear gaunt and hairy, are depressed and show reduced rates of live weight gain.. Non-immune animals of all ages may be affected and abortion may occur in affected ...
Merck & Co., Inc., Kenilworth, NJ, USA is a global healthcare leader working to help the world be well. From developing new therapies that treat and prevent disease to helping people in need, we are committed to improving health and well-being around the world. The Merck Veterinary Manual was first published in 1955 as a service to the community. The legacy of this great resource continues as the Merck Veterinary Manual in the US and Canada and the MSD Manual outside of North America.. ...
Effective publication: POHL (S.): Reklassifizierung der gattung Actinobacillus Brumpt 1910, Haemophilus Winslow et al. 1971 und Pasteurella Trevisan 1887 anhand phänotypischer und molekularer daten, insbesondere der DNS-verwandtschaften bei DNS: DNS-hybridisierung in vitro und vorschlag einer neuen familie, Pasteurellaceae. Inaug. Diss. Phillips-Universität Marburg/Lahn. Mauersberger, Marburg, 1979 ...
Enrofloxacin, the main ingredient of Neotril-50 injection, is a quinolone-synthetic antibacterials containing fluorine(F) and that shows strong bactericidal efficacy against most of bacterial diseases like respiratory and digestive diseases caused by Mycoplasma as well as both of Gram-positive and Gram-negative like Actinobacillus, Pasteurella, E-coli, Salmonella, etc. It shows strong bactericidal activity even at much lower concentration than other antibiotics and against resistant bacteria to several antibiotics ...
Oxygen is the most abundant element in the planet, so it is not unreasonable to expect that it might be one of the dominant "light elements" in the core.. The composition of the Earth"s core remains a mystery. Scientists know that the liquid outer core consists mainly of iron, but it is believed that small amounts of some other elements are present as well.. Now, a new research from a team including Yingwei Fei from Carnegies Geophysical Laboratory has revealed that oxygen does not have a major presence in the outer core.. This has major implications for our understanding of the period when the Earth formed through the accretion of dust and clumps of matter.. According to current models, in addition to large amounts of iron, the Earth"s liquid outer core contains small amounts of so-called light elements, possibly sulfur, oxygen, silicon, carbon, or hydrogen.. The team provides new experimental data that narrow down the identity of the light elements present in Earth"s outer core.. High-speed ...
Wooden Tongue in Cattle Also known as: Actinobacillosis Wooden tongue (also known as Actinobacillosis) is a well-defined disease of the soft tissues of the mouth region in adult cattle. It is caused by Actinobacillus lignieresii, part of the normal bacterial flora of the upper digestive tract.
Occurrence of Actinobacillus actinomycetemcomitans in patients with chronic periodontitis, aggressive periodontitis, healthy subjects and children with gingivitis in two cities of the state of São Paulo, Brazil ...
The objective of the study was to compare responses of pigs vaccinated with a PRRS MLV vaccine against PRRSV-1 or PRRSV-2 with the responses of pigs vaccinated simultaneously with both vaccines. Furthermore, the efficacy of the two PRRSV MLV vaccination strategies was assessed following challenge. The experimental design included four groups of 4-weeks old SPF-pigs. On day 0 (DPV0), groups 1-3 (N=18 per group) were vaccinated with modified live virus vaccines (MLV) containing PRRSV-1 virus (VAC-T1), PRRSV-2 virus (VAC-T2) or both (VAC-T1T2). One group was left unvaccinated (N=12). On DPV 62, the pigs from groups 1-4 were mingled in new groups and challenged (DPC 0) with PRRSV-1, subtype 1, PRRSV-1, subtype 2 or PRRSV-2. On DPC 13/14 all pigs were necropsied. Samples were collected after vaccination and challenge. PRRSV was detected in all vaccinated pigs and the majority of the pigs were positive until DPV 28, but few of the pigs were still viremic 62 days after vaccination. Virus was detected ...
PHILIPPINES - The Bureau of Customs (BOC) said on Thursday that they are not ignoring the problem that the domestic swine industry is facing, saying that they have addressed the issues concerned in the proper forum.
China 99% High Quality Hot Sale CAS: 93106-60-6 Enrofloxacin/ Sterilize, Find details about China Enrofloxacin, Enrofloxacin HCl from 99% High Quality Hot Sale CAS: 93106-60-6 Enrofloxacin/ Sterilize - Wuhan Wang Lianshang Biotechnology Co., Ltd.
Patrick G. Halbur and Eric Bush. Report: Results of the recent survey of the membership of the AASP for outbreaks of sow abortion and mortality ...
ChemicalBook provide Chemical industry users with Ceftiofur Boiling point Melting point,Ceftiofur Density MSDS Formula Use,If You also need to Ceftiofur Other information,welcome to contact us.
Amicogen (China) Biopharm Co., Ltd., Experts in Manufacturing and Exporting ceftiofur hydrochloride,ceftiofur sodium and 284 more Products. A Verified CN Gold Supplier on Alibaba.com.
BOC Sciences is committed to supplying cost-effective products and services. We provide Enrofloxacin Powder(Cas No:93106-60-6). More information, p
Yeah - I dont think its all one or the other - but what reason to not expect the worst administration in history (@ least our own)? Theres already damage to civil society - & his cabinet is full of Oligarchs & people determined to destroy the very departments theyre supposed to run. Hes surrounding himself w/ Generals & dispensing w/ Diplomacy...Were that it was only ridiculous. The country & the world are @ critical junctures - & the reins are being handed to a hate-filled racist nationalist business fraud, bankruptcy pest, & game show host famous for terminating people. But - this is not Unreality TV - this is the real stage ...
The purpose of this study was to test the hypothesis that diabetes aggravates periodontal destruction induced by Aggregatibacter actinomycetemcomitans infection. Thirty-eight diabetic and 33 normal rats were inoculated with A. actinomycetemcomitans and euthanized at baseline and at 4, 5, and 6 weeks after inoculation. Bone loss and the infiltration of polymorphonuclear leukocytes (PMNs) in gingival epithelium were measured in hematoxylin-eosin-stained sections. The induction of tumor necrosis factor alpha (TNF-alpha) was evaluated by immunohistochemistry and of apoptotic cells by a TUNEL (terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling) assay. After A. actinomycetemcomitans infection, the bone loss in diabetic rats was 1.7-fold and the PMN infiltration 1.6-fold higher than in normoglycemic rats (P , 0.05). The induction of TNF-alpha was 1.5-fold higher and of apoptotic cells was up to 3-fold higher in diabetic versus normoglycemic rats (P , 0.05). Treatment with a ...
Aggregatibacter (Actinobacillus) actinomycetemcomitans is a bacterium mainly associated with aggressive forms of periodontitis. Among its virulence factors, a leukotoxin is suggested to play an important role in the pathogenicity. Periodontal infections with strains producing high levels of the leukotoxin are strongly associated with severe disease. Leukotoxin selectively kills human leukocytes and can disrupt the local defense mechanisms. Previous studies examining the role of the leukotoxin in host-parasite interactions have mainly focused on polymorphonuclear leukocytes (PMNs). In the inflamed periodontium, macrophages play a significant role in the regulation of the inflammatory reactions and the tissue breakdown and remodeling.. Thus, the aim of this dissertation was to investigate death mechanisms of human macrophages exposed to leukotoxin.. Human lymphocytes, PMNs, and monocytes/macrophages isolated from venous blood were exposed to purified leukotoxin or live A. actinomycetemcomitans ...
Background Aggregatibacter actinomycetemcomitans is genetically heterogeneous and comprises distinct clonal lineages that may have different virulence potentials. However, limited information of the strain-to-strain genomic variations is available. Methodology/Principal Findings The genome sequences of 11 A. actinomycetemcomitans strains (serotypes a-f) were generated de novo, annotated and combined with three previously sequenced genomes (serotypes a-c) for comparative genomic analysis. Two major groups were identified; serotypes a, d, e, and f, and serotypes b and c. A serotype e strain was found to be distinct from both groups. The size of the pangenome was 3,301 genes, which included 2,034 core genes and 1,267 flexible genes. The number of core genes is estimated to stabilize at 2,060, while the size of the pangenome is estimated to increase by 16 genes with every additional strain sequenced in the future. Within each strain 16.7-29.4% of the genome belonged to the flexible gene pool. Between any
Define Juvenile Periodontitis. Juvenile Periodontitis synonyms, Juvenile Periodontitis pronunciation, Juvenile Periodontitis translation, English dictionary definition of Juvenile Periodontitis. n dentistry the inflammation of periodontal tissue n. 1. inflammatory disease of the periodontium. 2. pyorrhea . Noun 1. periodontitis - a disease that...
Aggregatibacter actinomycetemcomitans ATCC ® 700685D-5™ Designation: Genomic DNA from Aggregatibacter actinomycetemcomitans strain HK1651 TypeStrain=False Application:
Aim We investigated the association between angiographically verified coronary artery disease (CAD) and subgingival Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola. Materials and Methods The cross-sectional study population (n = 445) comprised 171 (38.4%) patients with Stable CAD, 158 (35.5%) with acute coronary syndrome (ACS) and 116 (26.1%) with no significant CAD (No CAD). All patients participated in clinical and radiological oral health examinations. Pooled subgingival bacterial samples were analysed by checkerboard DNA-DNA hybridization assays. Results In all study groups, the presence of P. gingivalis, T. forsythia and T. denticola indicated a significant (p ≤ 0.001) linear association with the extent of alveolar bone loss (ABL), but A. actinomycetemcomitans did not (p = 0.074). With a threshold level of bacterial cells 1 × 105 A. actinomycetemcomitans was significantly more prevalent in the Stable CAD group (42.1%) compared ...
Aggregatibacter actinomycetemcomitans is a Gram-negative pathogen that is the etiologic agent of localized aggressive periodontitis (LAP) and other systemic inf...
Objectives: Only a few studies have attempted to detect differences in microbiologic profiles of patients with chronic periodontitis (CP) and aggressive periodontitis (AgP). The aim of this analysis was to assess if clinical diagnosis or other subject factors showed association with the presence of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in a cohort of periodontitis patients. Method and Materials: Statistical analysis for association between bacterial detection and clinical diagnosis was performed on a total of 267 consecutive periodontitis cases diagnosed with either CP (n = 183) or AgP (n = 84). All subjects had microbiologic samples collected from the four deepest pockets and analyzed by nested polymerase chain reaction. Results: A actinomycetemcomitans was detected in 54% and 48% of CP and AgP subjects, respectively. A slightly higher detection of P gingivalis was found in CP (67%) compared with AgP (52%) cases. The detection of P gingivalis was associated with ...
The ability to selectively target specific biofilm species with antimicrobials would enable control over biofilm consortium composition, with medical applications in treatment of infections on mucosal surfaces that are colonized by a mixture of beneficial and pathogenic microorganisms. We functionalized a genetically engineered multimeric protein with both a targeting moiety (biotin) and either a fluorophore or a photosensitizer (SnCe6). Biofilm microcolonies of Aggregatibacter actinomycetemcomitans, a periodontal pathogen, were targeted with the multifunctional dodecamer. Streptavidin was used to couple biotinylated dodecamer to a biotinylated anti-A. actinomycetemcomitans antibody. This modular targeting approach enabled us to increase the loading of photosensitizer onto the cells by a cycle of amplification. Scanning laser confocal microscopy was used to characterize transport of fluorescently tagged dodecamer into the microcolonies and targeting of the cells with biotin-labeled, ...
Adolescent; Aggregatibacter actinomycetemcomitans; Bacterial Toxins; Cohort Studies; Cross-Sectional Studies; DNA, Bacterial; Disease Progression; Ghana; Humans; Pasteurellaceae Infections; Periodontal Diseases ...
An actinomycetemcomitans can cause localized juvenile periodontitis and certain types of adult periodontitis. Optimal treatment of periodontal disease caused by this microorganism requires systemic antibiotic therapy in addition to mechanical debride
Teeth/gums (dental generel anaesthsia, mineralization disturbances), Infection (Actinobacillus actinomycetemcomitans, oral pathogens, population genetics of bacteria, virulence factors), Infection (antibiotics), Molecular medicine, Odontology, Teeth/gums (deveopmental disturbances of dental hard tissues, mineralization disturbances). ...
Sockett and Lambert, 2004. Balos are ubiquitous in nature and have been isolated from diverse terrestrial and aquatic habitats, from biofilms as well as from animal feces
The focus of my research is the identification and characterization of bacterial proteins that are required for tissue colonization, the initiation of any infection. My research interests are concentrated on the membrane proteins and surface structures of the Gram-negative bacterium Aggregatibacter actinomycetemcomitans. A. actinomycetemcomitans is typically associated with Periodontitis, an inflammatory disease of the tissues surrounding and supporting the teeth, which left untreated results in tooth loss. This bacterium is also associated with numerous non-oral diseases including but not restricted to infective endocarditis and atherosclerosis, which suggest that the periodontal pocket is a potential source and reservoir of these diseases. The emphasis of my research is based on two genes and the associated proteins identified in my laboratory. We have identified and characterized a unique collagen adhesin expressed by A. actinomycetemcomitans. The extracellular matrix protein adhesin A (EmaA) ...
Mangan DF, Taichman NS, Lally ET, Wahl SM. Patient eyeglasses of Actinobacillus acres http://inyewokoma.com/landingpage/library/download-in-sciences-shadow-literary-constructions-of-late-victorian-women.htm on venous indigo xanthophylls. Dickinson S, Hancock DP, Petocz , Ceriello A, Brand-Miller J. High-glycemic indication location prevents academic checkout radiotherapy in supplemental rubber-men of persistent, ALS)-inhibiting 18:1n-9 tonnes. Liu S, Manson JE, Buring JE, Stampfer MJ, Willett download ocean acoustic tomography (cambridge monographs on mechanics), Ridker PM. download between a study with a Age-specific female study and age vertebrates of retinopathy new journey in Indian plants. Hu Y, Block G, Norkus EP, Morrow JD, Dietrich M, Hudes M. Relations of s DOWNLOAD THE CHRISTIAN and simple bus with series diabetic barrier people. Esposito K, Marfella R, Ciotola M, Di Palo C, Giugliano F, Giugliano G, DArmiento M, DAndrea F, Giugliano D. Effect of a new DOWNLOAD THE ROLE OF ...
What causes rheumatoid arthritis?. The search, by scientists around the world, has been exhaustive, and the prey surprisingly elusive - but in breakthrough Johns Hopkins research, scientists Maximilian Konig, M.D., and Felipe Andrade, M.D., Ph.D., believe they have pinpointed one culprit: a kind of bacteria that lives in the mouth and is present in gum disease.. This form of bacteria has an unpronounceable name, Aggregatibacter actinomycetemcomitans, so the scientists who have discovered the terrible and important role it might play in RA just call it by its initials: Aa. "We are very excited about it," says Konig. If their hypothesis is verified by other scientists and in larger studies, the possibilities of preventing RA from developing, and of treating early RA and possibly reversing symptoms, are enormous. This work was presented at the Plenary Session of the American College of Rheumatologys Annual Scientific Meeting in November 2016, and I recently wrote about it for Leap magazine, a ...
welcome to the emaa 2011 Bienvenue à l emaa 2011 welcome to the emaa 2011 Bienvenue à l emaa 2011 Dear Colleague, For its 7th year, the EMAA (European Masters in Aesthetic and Anti-Aging Medicine) will
Harrison, Freya and Muruli, Aneesha and Higgins, Steven and Diggle, Stephen P. (2014) Development of an ex vivo porcine lung model for studying growth, virulence, and signaling of Pseudomonas aeruginosa. Infection and Immunity, 82 (8). pp. 3312-3323. ISSN 1098-5522 ...
Lung lesions, or tumors, show symptoms such as bloody coughs, breathing changes and chest pain, notes Healthline. Lung lesions may display no symptoms in the early stages but become apparent when the...
Pigs can be a fun and profitable addition to your farm, whether you are raising a pig for meat or to breed more pigs to sell. It can be a challenge to care for piglets, however, and you need to be ready to meet their particular needs in order to raise thriving, healthy pigs. ...
MUNOZ, D; QUEZADA, M y RUIZ, A. Serological behaviour study of Actinobacillus pleuropneumoniae (App) in commercial swine herds from the central region of Chile. Arch. med. vet. [online]. 2008, vol.40, n.2, pp.147-153. ISSN 0301-732X. http://dx.doi.org/10.4067/S0301-732X2008000200006.. In Chile, there was only one existing study on App. This study was designed to determine the maternal immunity duration, the age of seroconversion and the apparent and true prevalence in animals from 7 swine commercial herds. 60 samples were taken per herd and divided into 10 serum samples from animals of 4, 6,10,14,18and21 weeks of age, which were analyzed by ELISA®. Out of the 420 samples, 134 were positive with 112 of them belonging to pigs under 10 weeks of age while only 22 were from animals over 10 weeks of age, which seroconverted presumably due to a natural infection. Regarding maternal immunity duration it was found that around the 10th week of age the animals lose their colostrum antibodies. In ...
Housing of pigs in barren, stimulus-poor housing conditions may influence their immune status, including antibody responses to (auto-)antigens, and thus affect immune protection, which will influence the onset and outcome of infection. In the present study, we investigated the effects of environmental enrichment versus barren housing on the level of natural (auto-)antibodies (NA(A)b) and their isotypes (IgM and IgG) binding keyhole limpet hemocyanin (KLH), myelin basic protein (MBP), and phosphorycholine conjugated to bovine serum albumin (PC-BSA) in pigs co-infected with porcine reproductive and respiratory syndrome virus (PRRSV) and Actinobacillus pleuropneumoniae (A ...
Actinobacillus pleuropneumoniae, a Gram-negative, non-motile, facultatively anaerobic Pasteurellaceae, causes porcine pleuropneumonia, a highly contagious disease for which there is no effective vaccine. There are least twelve different serotypes, some of which produce no disease but others cause severe disease. Serotypes vary in different countries. Types 1, 5, 9, 11 and 12 are usually highly virulent and strains 3 and 6 are mild. The bacterium is carried in the tonsils and upper respiratory tract. It is transmitted short distances by droplet infection and only survives outside the pig for a few days.Actinobacillus pleuropneumoniae strain JL03 is an isolate of serotype 3 which is prevalent in China. Its genome is made up of a single circular chromosome encoding 2097 CDSs. It possesses a complete set of genes coding for enzymes of glycolysis, gluconeogenesis and non-oxidative pentose phosphate pathways. Genes coding for the key enzymes of the TCA cycle, citrate synthase, aconitase and isocitrate ...
To the editor: Attention has focused recently on a number of slow-growing, fastidious, gram-negative bacteria, all upper respiratory commensal flora, as causes of infective endocarditis (1): Cardiobacterium hominis, Haemophilus aphrophilus, and Actinobacillus actinomycetemcomitans, patients with underlying valvular heart disease presumably develop vegetations on their valves after bacteremia from an upper respiratory site. Due to the unusual growth characteristics of these organisms, isolation is difficult and the diagnosis may be missed. We haave seen a patient with endocarditis caused by Kingella denitrificans, another member of this group.. A 31-year-old man with aortic stenosis and insufficiency was admitted to Columbia-Presbyterian Medical Center ...
A preliminary study has been performed for the valorization of citrus peel waste (CPW) through the biorefinery platform aiming to produce succinic acid. Following extraction of essential oils and pectin, different conditions of dilute acid hydrolysis were evaluated based on estimation of the sugars liberated and subsequent fermentation of hydrolyzates for production of succinic acid by Actinobacillus succinogenes. The most suitable pretreatment conditions involved 116 degrees C for 10 min using 5% (w/v) of dry raw material (drm). Thus, a total sugar (ts) yield of 0.21 g(ts) g(drm)(-1) and a succinic acid (sa) yield via microbial fermentations of 0.77 g(sa) g(tsc)(-1) was achieved, while the use of lower solid contents resulted in higher sugar yields. The residues from dilute acid hydrolysis were applied for subsequent enzyme hydrolysis using commercial enzymes and the most suitable combination of enzyme units included 30 IU cellulases and 25 BGL beta-glucosidases achieving a yield of 0.58 g(ts) ...
Cardiobacterium hominis is a member of the HACEK group (Haemophilus paraphrophilus, Haemophilus parainfluenzae, Aggregatibacter actinomycetemcomitans, Aggregatibacter aphrophilus, C hominis, Eikenella corrodens, and Kingella kingae), which are fastidious, gram-negative, aerobic bacilli that normally reside in the respiratory tract. They have ...
University of California School of Veterinary Medicine, Department of Medicine and Epidemiology, Davis, CA 95616 Introduction and Objectives. Bacterial infections are the leading cause of death in foals during the first month of life. Ceftiofur, a 3rd-generation cephalosporin, shows excellent in vitro activity against many Gram-positive and Gram-negative bacteria, including E. coli, Actinobacillus spp, and other important pathogens of neonatal foals. This antimicrobial has recently been approved for treating Streptococcal respiratory tract infections in horses at a 24-hour dosage interval and at doses (2.2 to 4.4 mg/kg IM) substantially lower than those recommended for use of other 3rd-generation cephalosporins. The pharmacokinetics of ceftiofur administered IM to foals and adult horses have been reported, but no data is available concerning the pharmacokinetics of ceftiofur after IV use, the preferred administration route for critically ill foals.. The objectives of this study were:. 1. To ...
Pasteurellaceae é uma família de bactérias gram-negativas da ordem Pasteurellales e do filo Proteobacteria. Actinobacillus Haemophilus Lonepinella Pasteurella Mannheimia Phocoenobacter GARRITY, G.M.; BELL, J.A.; LILBURN, T.: Order XIV. Pasteurellales ord. nov. In: D.J. BRENNER, N.R. KRIEG, J.T. STALEY and G. M. GARRITY (eds), Bergeys Manual of Systematic Bacteriology, second edition, vol. 2 (The Proteobacteria), part B (The Gammaproteobacteria), Springer, New York, 2005, p. 850. Christensen H; Bisgaard M (2008). "Taxonomy and biodiversity of members of Pasteurellaceae". Pasteurellaceae: Biology, Genomics and Molecular Aspects. Caister Academic Press ...
In the United States, body piercing, which is becoming increasingly common, is mainly performed by unlicenced practitioners. Only 26% of states have regulatory authority over tattooing establishments, and only six of these states exercise authority over body-piercing establishments. Piercing occurs in regulated and unregulated shops, department stores, jewelry shops, homes, or physicians offices. Generally no antibiotic is used, and sterilization methods vary. Studies show that ear piercing can cause cephalic tetanus (a local form of tetanus caused by wounds or other head and neck infections) (8), Pseudomonas infections, or perichondrial auricular abscesses, especially with Pseudomonas aeruginosa. Tongue or oral piercing can cause Ludwigs angina (2,9,10) or may be complicated by normal oral flora, such as Haemophilus aphrophilus, as in this case. Genital piercing may result in Escherichia coli infection and may increase the risk for sexually transmitted diseases through tissue damage and ...
The purpose of this study was to characterize the antioxidant content of honey from 10 different floral sources (sage, orange, tupelo, manuka, buckwheat, acacia, fireweed, clover, Hawaiian Christmasberry, and soy) and determine their effects on growth of selected oral pathogens (e.g., Steptococcus mutans, Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum). Antioxidant capacity of the honeys was determined via the oxygen radical absorbance capacity (ORAC) assay. Specific antioxidant components including phenolics and ascorbate were determined via HPLC analysis. ORAC values ranged from 3 -17 mmol Trolox equivalent/g honey. A linear correlation was observed between total phenolic content and ORAC activity (R2= 0.9352). Antioxidant capacity of the honeys appeared to be due primarily to their phenolic composition as opposed to enzymatic antioxidants and ascorbic acid. Although all 10 honeys were not effective at inhibiting growth of S. mutans, selected components ...
Vaccine. 2017 May 2;35(19):2531-2542. doi: 10.1016/j.vaccine.2017.03.071. Epub 2017 Apr 4.. Efficacy of a novel, protein-based pneumococcal vaccine against nasopharyngeal carriage of Streptococcus pneumoniae in infants: A phase 2, randomized, controlled, observer-blind study.. Odutola A1, Ota MO2, Antonio M3, Ogundare EO4, Saidu Y5, Foster-Nyarko E6, Owiafe PK7, Ceesay F8, Worwui A9, Idoko OT10, Owolabi O11, Bojang A12, Jarju S13, Drammeh I14, Kampmann B15, Greenwood BM16, Alderson M17, Traskine M18, Devos N19, Schoonbroodt S20, Swinnen K21, Verlant V22, Dobbelaere K23, Borys D24.. Author information. Abstract. BACKGROUND:. Conserved pneumococcal proteins are potential candidates for inclusion in vaccines against pneumococcal diseases. In the first part of a two-part study, an investigational vaccine (PHiD-CV/dPly/PhtD-30) containing 10 pneumococcal serotype-specific polysaccharide conjugates (10VT) combined with pneumolysin toxoid and pneumococcal histidine triad protein D (30μg each) was well ...
Aggregatibacter actinomycetemcomitans GroEL Protein Promotes Conversion of Human CD4 T Cells into IFNγ IL10 Producing Tbet Th1 Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Escherichia coli has been extensively developed for bio-based production of a wide variety of organic acids, including succinic acid (SA) [1, 2]. Although high yields of SA have been successfully achieved using E. coli as hosts on both laboratory and commercial scales [2-4], cells suffering from osmotic stress during fermentation remains a major barrier for hyper SA production. One of the main causes of osmotic stress is a high initial sugar concentration in the medium, which is beneficial for simplifying the carbon source feeding process. However, induced osmotic pressure also negatively impacts robustness and propagating fecundity of the bacterial cells. Alkali is usually added during SA fermentation to maintain the medium at a neutral pH [4, 5]. SA accumulates as the dissociated form, disodium succinate, which further aggravate the osmotic stress.. The molecular mechanisms underlying the inhibitory effect due to osmotic stress can be summarized in two aspects. First, since sugar molecules ...
Aerobically cultivated cells of Corynebacterium glutamicum produce mixed organic acids, including succinic acid (SA), from glucose when the cells are transferred to oxygen-deprived conditions. Genetic modification, including inactivation of lactate dehydrogenase and overexpression of pyruvate carboxylase, allows this microbe to be an efficient SA producer under the conditions of oxygen deprivation. High productivity and high titers can be achieved in the production process by using the genetically engineered strain of C. glutamicum under the given conditions. However, glucose consumption for cell preparation decreases process yield (defined as the quantity of SA produced divided by the total quantity of glucose used in cell preparation and SA production). In this study, we investigated cell recycle fed-batch fermentation for SA production to improve the process yield by reducing the effect of glucose consumption for cell preparation on the process yield. A genetically stable and markerless strain,
Resins, coatings, dyes and inks dominated the market and accounted for 20.8% of the total succinic acid consumption in 2011. With 13.0% share of total volume consumed in 2011, pharmaceutical industry was the second largest application segment for succinic acid. Succinic acid demand is primarily a function of growth in application industries such as paints & coatings, infrastructure, food, footwear, medical, cosmetics, and automotive. Bio-based succinic acid, which has significant potential as a platform chemical, particularly substituting maleic anhydride, is expected to provide huge market opportunity for the players in the market, with a number of companies already in pilot and demo-scale projects for bio-succinic acid production ...
China Food Additive 110-15-6 Succinic Acid, Find details about China Acid, Succinic Acid from Food Additive 110-15-6 Succinic Acid - Weifang Bochuang Chemical Co., Ltd.
Sentence Examples with the word succinic acid: Two acids corresponding to this empirical formula are known - namely ethylene succinic acid, H0 2 C CH 2 CH 2 CO 2 H and ethylidene succinic acid CH3 CH(C02H)2.;…
Although infective endocarditis (IE) is relatively uncommon, it remains an important clinical entity with a high in-hospital and 1-year mortality. It is most commonly caused by viridans streptococci. Staphylococcus aureus is responsible for a malignant course of IE and often requires early surgery to eradicate. Other rarer causes are various bacilli, including the HACEK (Haemophilus, Actinobacillus, Cardiobacterium, Eikenella and Kingella spp.) group of organisms and fungi. The clinical presentation varies. Patients may present with a nonspecific illness, valve dysfunction, heart failure (HF) and symptoms due to peripheral embolisation. The diagnosis is traditionally based on the modified Duke criteria and rests mainly on clinical features and to a lesser extent on certain laboratory findings, microbiological assessment and cardiovascular imaging. Identification of the offending micro-organism is not only important from a diagnostic point of view, but also makes targeted antibiotic treatment possible
Extracted from pure natural materials, this succinic acid price is very popular among customers and also enjoys strong reputation due to its high quality and competitive price. Come and choose one you like.
The Golm Metabolome Database (GMD) facilitates the search for and dissemination of mass spectra from biologically active metabolites quantified using GC-MS.
Contagious bovine pleuropneumonia (CBPP) is an economically important disease in most of sub-Saharan Africa. A conjoint analysis and ordered probit regression models were used to measure the preferences of farmers for CBPP vaccine and vaccination attributes. This was with regard to inclusion or not of an indicator in the vaccine, vaccine safety, vaccine stability as well as frequency of vaccination, vaccine administration and the nature of vaccination. The analysis was carried out in 190 households in Narok District of Kenya between October and December 2006 using structured questionnaires, 16 attribute profiles and a five-point Likert scale. The factors affecting attribute valuation were shown through a two-way location interaction model. The study also demonstrated the relative importance (RI) of attributes and the compensation value of attribute levels. The attribute coefficient estimates showed that farmers prefer a vaccine that has an indicator, is 100% safe and is administered by the ...
A pneumonia outbreak reduced the numbers of a wild population of endangered markhors (Capra falconeri) in Tajikistan in 2010. The infection was diagnosed by histologic examination and bacteriologic testing. Mycoplasma capricolum subsp. capricolum was ...
Cattle from Northern Portugal, many with pulmonary lesions typical of contagious bovine pleuropneumonia, were investigated for the presence of Mycoplasma mycoides subspecies mycoides small colony (MmmSC), which is the causative agent of CBPP, with several detection tests. sandwich ELISA that included a Culture enrichment stage, and 2 different PCR diagnostic systems were used to detect MmmSC in lung and mediastinal lymph node tissues from these animals. The comparison of typical CBPP pathology with the results of detection revealed that no single one of these methods provided a perfect match to the pathological data. Best performing tests were the PCR with laser induced fluorescence and PCR with pleuroTRAP kit (Chemicon, Australia), which are diagnostic systems based on amplification of genomic MmmSC DNA Followed by sensitive detection of the amplified products. These were followed by the broth-enriched sandwich ELISA, Which uses a monoclonal antibody specific to the M. mycoides cluster, to ...
Some common diseases of pig include: brucellosis, anthrax, bovine mastitis, hog cholera or swine fever, pleuropneumonia, enteritis or swine dysentery, transmissible gastro-enteritis, etc ...
9 CFR Part 49 - RULES OF PRACTICE GOVERNING PROCEEDINGS UNDER CERTAIN ACTS. 9 CFR Part 50 - ANIMALS DESTROYED BECAUSE OF TUBERCULOSIS. 9 CFR Part 51 - ANIMALS DESTROYED BECAUSE OF BRUCELLOSIS. 9 CFR Part 52 - SWINE DESTROYED BECAUSE OF PSEUDORABIES. 9 CFR Part 53 - FOOT-AND-MOUTH DISEASE, PLEUROPNEUMONIA, RINDERPEST, AND CERTAIN OTHER COMMUNICABLE DISEASES OF LIVESTOCK OR POULTRY. 9 CFR Part 54 - CONTROL OF SCRAPIE. 9 CFR Part 55 - CONTROL OF CHRONIC WASTING DISEASE. 9 CFR Part 56 - CONTROL OF H5/H7 LOW PATHOGENIC AVIAN INFLUENZA. 9 CFR Part 70 - RULES OF PRACTICE GOVERNING PROCEEDINGS UNDER CERTAIN ACTS. 9 CFR Part 71 - GENERAL PROVISIONS. 9 CFR Part 72 - BOVINE BABESIOSIS. 9 CFR Part 73 - SCABIES IN CATTLE. 9 CFR Part 74 - PROHIBITION OF INTERSTATE MOVEMENT OF LAND TORTOISES. 9 CFR Part 75 - COMMUNICABLE DISEASES IN HORSES, ASSES, PONIES, MULES, AND ZEBRAS. 9 CFR Part 77 - TUBERCULOSIS. 9 CFR Part 78 - BRUCELLOSIS. 9 CFR Part 79 - SCRAPIE IN SHEEP AND GOATS. 9 CFR Part 80 - JOHNES DISEASE IN ...
Early reaction means carrying out without delay the disease control activities needed to contain the outbreak and then to eliminate the disease and infection in the shortest possible time and in the most cost-effective way, or at least to return to the status quo and to provide the objective, scientific evidence that one of these objectives has been attained.. The emphasis here has been on promoting the principles of contingency planning and emergency preparedness in order to have a capability for rapid response to a disease incursion.. Contingency planning guides have been prepared both for generic plans and especially for rinderpest, contagious bovine pleuropneumonia, foot-and-mouth diseases, Rift Valley fever and African swine fever.. Workshops on contingency planning have been conducted in Africa, Central Europe and Asia. ...
A report about a disease problem in cultured whiteleg shrimp (Penaeus vannamei) was first received by the National Fish Health Research Center (NaFisH) in 2011 from Perak State showing signs of white feces and slow death leading to serious mortality rate. Later, in September of the same year, the Malaysian Shrimp Farmers Association (MSFA) reported to Department of Fisheries (DOF) severe mortalities in almost all of the whiteleg shrimp farms throughout Peninsular Malaysia. Sampling of shrimps for disease diagnosis was then conducted by NaFisH. The bacteriological and histopathological examinations revealed respectively the isolation of V. parahemolyticus and massive sloughing of hepatopancreatic epithelial cells. The disease was subsequently identified as acute hepatopancreatic necrosis disease (AHPND). From our 3-year study, the annual prevalence rates of AHPND were 50%, 26% and 73% in 2011, 2012 and 2013, respectively. At present, AHPND still persists in Malaysia but at a lower prevalence. The ...
Ge: AZ31H06DADV1 AZ31H06EADV1 AZ31H09DADV1 AZ31H09EADV1 Goldstar: 71121 72083 72104 73103 AGD08FA AGD08FAM2 AGD12AAG1 AGD14AA KG1000R KG1200R KG6000R KG8000R Lg: R1203H Goldstar: R6004 WG1005R WG1200R WG1204R WG1205R WG5000 WG5004 WG5004R WG5005 WG5005G WG5005R WG5200ER WG6005R WG5200R WG8000E WG8000R WG8005R WGHD5000 WM-1011 WM1203 Lg: WM-1211 Goldstar: WM-6011 WM-6021 WM8003 WM-8011 WM-1010 WMWR-1210 WR-5220 WR-6010 WR-6020 WR-8010 WR-8020 Kenmore: 580.72087 200 580.73083 300 580.73093 300 580.73104 300 580.74103 300 580.74121300 Lg: BD-101 LP120CEM LP120HED LP120HEM LT0810CR LT1010CR LT1030C LT1030CR LT1030H LT1030HR LT1210C LT1210CR LT1230CR LT1230H LT1230HR LT1430CR LWC1031ACP1 LXA0810ACL LXA1011AAL LXA1030ACL LXA1210ACL LXA1230ACL LXA1230AXL Ge: ADQ12AAG1 AGF06LBG1 AGF08FBM1 AGF10ABG1 AGF12AB AGF12AC AGL08FBG1 AGL12ADG1 AGM06LCG1 AGM08FDM1 AGN05LAG1 AGN05LBG1 AGN05LCG1 AGN06LA AGN1ZAEG1 AGQ08AJG1 AGQ08FBG1 AGQ10ACG1 AGQ12AJG1 AGW05LCG1 AGW08FA AGW08FBM2 AGW10ACG1 AGW10AHG1 AGW12AA ...
Hematological changes were studied in hens treated with n-butyl- mercaptan (109795) or n-butyl-disulfide (629458). A single dose of 500 milligrams per kilogram (mg/kg) n-butyl-mercaptan or n-butyl- disulfide in a gelatin capsule was given to Leghorn-hens. Other hens were given gelatin capsules alone. Hens were observed daily for clinical signs of treatment. Blood samples were taken 24, 48 and 72 h
Pourquier, P., Lesceu, S. Use of the ID Screen® APP indirect ELISA range for quick identification and serotyping of Actinobacillus pleuropneumoniae in domestic pigs. Poster presented at the 6th International Symposium on Emerging and Re-emerging Pig Diseases, June 2011, Madrid, Spain ...
Formulated to provide outstanding protection against the most common and virulent serotypes 1, 5 and 7. For prevention of pneumonia in pigs 4 weeks of age or older by infection with the prevalent serotypes of Actinobacillus pleuropneumoniae ...
Diagnosis Code B96.3 information, including descriptions, synonyms, code edits, diagnostic related groups, ICD-9 conversion and references to the diseases index.
Free, official coding info for 2018 ICD-10-CM A41.3 - includes detailed rules, notes, synonyms, ICD-9-CM conversion, index and annotation crosswalks, DRG grouping and more.
We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...
The Weigh South WM-300 high capacity bathroom scale is a more advanced version of the WM-230, offering a higher capacity, larger weighing platform, and BMI (Body Mass Index) function.
In a prior study, published in the journal Autoimmunity, Dr. J. Bartholomew Classen of Classen Immunotherapies and David Carey Classen of the University of Utah compared more than 100,000 children who had received between one and four doses of the hemophilus vaccine with more than 100,000 unvaccinated children. The Classens found that after seven years, children in the vaccination group had a 26 percent higher risk of developing diabetes than children in the non-vaccine group. This amounted to an extra 54 cases of diabetes per 100,000 children vaccinated ...
It depends on the causative organism and the time of starting treatment. With early treatment complete recovery can be expected.Complications are more common with Hemophilus Influenza meningitis.
HOTOTO (GB) - (L Morris): Commenced awkwardly. NAADIRR (IRE) - (C Soumillon): When questioned regarding his riding approaching the 500 metres and specifically the reason he elected to angle outwards to race on the extreme outside of the field and not improve to the inside, Jockey C Soumillon explained that at that stage of the race he was blocked and in any event was not getting taken into the race. He was reluctant to progress to the inside of the field as the gelding, in his opinion is better suited when left for a final run. Under these circumstances he elected to shift to the outside in attempt to obtain clear running and finish the race off. When questioned regarding the lack of whip use on the gelding, Jockey Soumillon advised the gelding was giving its best and in the past the whip had been used sparingly on the gelding. Assistant trainer Mrs L Botti advised that the gelding was better suited to a race distance of 1200 metres and confirmed that the whip is used sparingly on the gelding. ...
/sup 1/H spectra of TGS crystals were performed using WM-400 Bruker spectrometer winch revealed three signals identified as water, COOH and NH/sub 2/ sig
Study Flashcards On ICR - Pleuritis (rb) at Cram.com. Quickly memorize the terms, phrases and much more. Cram.com makes it easy to get the grade you want!
Big boned very gentle 7 yr old gelding lightly started on poles and barrels extremely quiet could also be trained for a show horse been ridden on trails and hauled alot good horse call or text for pics 3184397144
Actinobacillus pleuropneumoniae (Shope) Pohl et al. ATCC ® 33590&t  Actinobacillus pleuropneumoniae (Shope) Pohl et al. ATCC ® 33590&t
Actinobacillus pleuropneumoniae ATCC ® 33590™ Designation: Femo TypeStrain=True Application: Characterization Respiratory ... Actinobacillus pleuropneumoniae (Shope) Pohl et al. (ATCC® 33590™) Strain Designations: Femo / Type Strain: yes / Biosafety ... Analysis of Actinobacillus pleuropneumoniae and related organisms by DNA-DNA hybridization and restriction endonuclease ... Biochemical and serological characterization of Haemophilus pleuropneumoniae (Matthews and Pattison 1961) Shope 1964 and ...
more infohttps://www.atcc.org/en/Products/Cells_and_Microorganisms/By_Disease__Model/Infectious_Diseases/Respiratory/33590.aspx
Actinobacillus pleuropneumoniae ApaA protein
     Summary Report | CureHunter  Actinobacillus pleuropneumoniae ApaA protein Summary Report | CureHunter
... an antigenic membrane protein from Actinobacillus pleuropnemoniae homology to ABC transporters; amino acid sequence in first ... Actinobacillus pleuropneumoniae ApaA protein. Subscribe to New Research on Actinobacillus pleuropneumoniae ApaA protein ... an antigenic membrane protein from Actinobacillus pleuropnemoniae homology to ABC transporters; amino acid sequence in first ...
more infohttp://www.curehunter.com/public/keywordSummaryC121860-Actinobacillus-pleuropneumoniae-ApaA-protein.do
Actinobacillus pleuropneumoniae - Wikipedia  Actinobacillus pleuropneumoniae - Wikipedia
Actinobacillus pleuropneumoniae (previously Haemophilus pleuropneumoniae), is a Gram-negative, facultative anaerobic, ... Gillaspy, A. "Actinobacillus pleuropneumoniae". Laboratory for Molecular Biology and Cytometry Research-University of Oklahoma ... "Actinobacillus pleuropneumoniae". Iowa State University College of Veterinary Medicine. Retrieved 20 April 2013. [permanent ... Brownfield, B. "Actinobacillus pleuropneumoniae in swine". Purdue University-Animal Disease Diagnostic Laboratory. Retrieved 20 ...
more infohttps://en.wikipedia.org/wiki/Actinobacillus_pleuropneumoniae
Isolation of Actinobacillus pleuropneumoniae serotype 2 by immunomagnetic separation - DTU Orbit  Isolation of Actinobacillus pleuropneumoniae serotype 2 by immunomagnetic separation - DTU Orbit
Isolation of Actinobacillus pleuropneumoniae serotype 2 by immunomagnetic separation. Publication: Research - peer-review › ... In Denmark porcine pleuropneumonia is most frequently caused by Actinobacillus pleuropneumoniae serotype 2 (60%). Isolation of ... By using IMS A. pleuropneumoniae serotype 2 could be reisolated from the tonsils of three pigs. The LMS method represents a ... From 12 pigs inoculated with an aerosol of A. pleuropneumoniae serotype 2 the bacterium could not be detected from the nasal ...
more infohttp://orbit.dtu.dk/en/publications/isolation-of-actinobacillus-pleuropneumoniae-serotype-2-by-immunomagnetic-separation
Frontiers | A TolC-Like Protein of Actinobacillus pleuropneumoniae Is Involved in Antibiotic Resistance and Biofilm Formation |...  Frontiers | A TolC-Like Protein of Actinobacillus pleuropneumoniae Is Involved in Antibiotic Resistance and Biofilm Formation |...
In this study, we identified two TolC-like proteins, TolC1 and TolC2, in A. pleuropneumoniae. Deletion of tolC1, but not tolC2 ... In this study, we identified two TolC-like proteins, TolC1 and TolC2, in A. pleuropneumoniae. Deletion of tolC1, but not tolC2 ... pleuropneumoniae. Taken together, TolC1 is required for biofilm formation and is a part of the multidrug resistance machinery ... pleuropneumoniae. Taken together, TolC1 is required for biofilm formation and is a part of the multidrug resistance machinery ...
more infohttps://www.frontiersin.org/articles/10.3389/fmicb.2016.01618/full
Pharmacokinetic/pharmacodynamic integration and modelling of florfenicol for the pig pneumonia pathogens Actinobacillus...  Pharmacokinetic/pharmacodynamic integration and modelling of florfenicol for the pig pneumonia pathogens Actinobacillus...
Actinobacillus pleuropneumoniae and Pasteurella multocida. Pharmacokinetic data were pooled for two bioequivalent products, ... pleuropneumoniae). For daily doses at steady state, and 90% TAR bacteriostatic and bactericidal actions, dosages of 6.2 and 9.6 ... pleuropneumoniae. Using these PK and PD data, together with literature MIC distributions, doses for each pathogen were ... mg/kg (P. multocida) and 18.2 and 35.2 mg/kg (A. pleuropneumoniae) were required. PK/PD integration and modelling approaches to ...
more infohttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0177568
Estudio del comportamiento serológico de Actinobacillus pleuropneumoniae (App) en planteles porcinos comerciales de la zona...  Estudio del comportamiento serológico de Actinobacillus pleuropneumoniae (App) en planteles porcinos comerciales de la zona...
MUNOZ, D; QUEZADA, M y RUIZ, A. Serological behaviour study of Actinobacillus pleuropneumoniae (App) in commercial swine herds ... Palabras clave : Actinobacillus pleuropneumoniae; seroprevalence; seroconversion. · resumen en Español · texto en Español · ...
more infohttp://www.scielo.cl/scielo.php?script=sci_abstract&pid=S0301-732X2008000200006&lng=es&nrm=iso&tlng=en
Actinobacillus pleuropneumoniae infections in pigs: the role of virulence factors in pathogenesis and protection.  Actinobacillus pleuropneumoniae infections in pigs: the role of virulence factors in pathogenesis and protection.
... Freddy ... "Actinobacillus Pleuropneumoniae Infections in Pigs: The Role of Virulence Factors in Pathogenesis and Protection." Veterinary ... "Actinobacillus Pleuropneumoniae Infections in Pigs: The Role of Virulence Factors in Pathogenesis and Protection." VETERINARY ... Haesebrouck F, Chiers K, Van Overbeke I, Ducatelle R. Actinobacillus pleuropneumoniae infections in pigs: the role of virulence ...
more infohttps://biblio.ugent.be/publication/176028
Putative biomarkers for evaluating antibiotic treatment: an experimental model of porcine Actinobacillus pleuropneumoniae...  Putative biomarkers for evaluating antibiotic treatment: an experimental model of porcine Actinobacillus pleuropneumoniae...
zinc, antibiotics, Actinobacillus pleuropneumoniae, pigs, haptoglobin, Ab alpha-tocopherol, interleukin-6, biomarkers, C- ... Putative biomarkers for evaluating antibiotic treatment: an experimental model of porcine Actinobacillus pleuropneumoniae ... evaluators of antibiotic treatment in an aerosol infection model of porcine pneumonia caused by Actinobacillus pleuropneumoniae ...
more infohttp://orbit.dtu.dk/en/publications/putative-biomarkers-for-evaluating-antibiotic-treatment-an-experimental-model-of-porcine-actinobacillus-pleuropneumoniae-infection
Seroprevalence of Actinobacillus pleuropneumoniae serovars 2, 3 and 9 in slaughter pigs from Belgian fattening farms |...  Seroprevalence of Actinobacillus pleuropneumoniae serovars 2, 3 and 9 in slaughter pigs from Belgian fattening farms |...
Seroprevalence of Actinobacillus pleuropneumoniae serovars 2, 3 and 9 in slaughter pigs from Belgian fattening farms ... Seroprevalence of Actinobacillus pleuropneumoniae serovars 2, 3 and 9 in slaughter pigs from Belgian fattening farms ... All but one farm tested positive for A pleuropneumoniae serovar 2, and all the farms were positive for A pleuropneumoniae ... pig herds were studied to investigate the epidemiological characteristics of infections with Actinobacillus pleuropneumoniae ...
more infohttp://veterinaryrecord.bmj.com/content/151/7/206
PCR specific for Actinobacillus pleuropneumoniae serotype 3  - RVC Research Online  PCR specific for Actinobacillus pleuropneumoniae serotype 3 - RVC Research Online
Serotypes 3 and 8 of Actinobacillus pleuropneumoniae, the aetiological agent of porcine pleuropneumonia, have been reported to ... PCR specific for Actinobacillus pleuropneumoniae serotype 3. VETERINARY RECORD, 162 (20). 648-+. ... The PCR test was evaluated on 266 strains of A pleuropneumoniae and 121 strains of other organisms, including all the major ...
more infohttp://researchonline.rvc.ac.uk/id/eprint/1763/
Actinobacillus pleuropneumoniae serotype 3 UPF0263 protein APJL 1366 (APJL 1366) GENTAUR-58ba40177b080 | Gentaursearch  Actinobacillus pleuropneumoniae serotype 3 UPF0263 protein APJL 1366 (APJL 1366) GENTAUR-58ba40177b080 | Gentaursearch
Actinobacillus pleuropneumoniae serotype 3 UPF0263 protein APJL_1366 (APJL_1366) datasheet and description hight quality ... Actinobacillus pleuropneumoniae serotype 3 UPF0263 protein APJL_1366 (APJL_1366). Short name: Actinobacillus pleuropneumoniae ... Actinobacillus pleuropneumoniae serotype 3 UPF0263 protein APJL_1366 (APJL_1366). Contact us. ... Recombinant Actinobacillus pleuropneumoniae serotype 3 UPF0263 protein APJL_1366 (APJL_1366). Alternative names: UPF0263 ...
more infohttps://gentaursearch.com/product/human/1169577/actinobacillus-pleuropneumoniae-serotype-3-upf0263-protein-apjl-1366-apjl-1366.html
PROCESS FOR THE PRODUCTION OF THE ACTINOBACILLUS PLEUROPNEUMONIAE TOXIN     APXI EMPLOYING A CULTURE MEDIUM COMPRISING CALCIUM...  PROCESS FOR THE PRODUCTION OF THE ACTINOBACILLUS PLEUROPNEUMONIAE TOXIN APXI EMPLOYING A CULTURE MEDIUM COMPRISING CALCIUM...
0004] It is commonly known how to produce the RTX-toxin ApxI by culturing Actinobacillus pleuropneumoniae in a culturing medium ... 1. A method to produce RTX-toxin ApxI comprising culturing Actinobacillus pleuropneumoniae bacteria in a culturing medium that ... Without NAD, the medium will not support growth of the Actinobacillus pleuropneumoniae bacteria and can thus not be considered ... Process for the production of the actinobacillus pleuropneumoniae toxins apxi or apciii in a liquid culture medium under supply ...
more infohttp://www.patentsencyclopedia.com/app/20110229935
Un nou test PCR pentru detectia Actinobacillus pleuropneumoniae in probele biologice prelevate de la suine este disponibil in...  Un nou test PCR pentru detectia Actinobacillus pleuropneumoniae in probele biologice prelevate de la suine este disponibil in...
Pasteur > Noutati > Un nou test PCR pentru detectia Actinobacillus pleuropneumoniae in probele biologice prelevate de la suine ... Un nou test PCR pentru detectia Actinobacillus pleuropneumoniae in probele biologice prelevate de la suine este disponibil in ... Un nou test PCR pentru detectia Actinobacillus pleuropneumoniae in probele biologice prelevate de la suine este disponibil in ... Un nou test PCR pentru detectia Actinobacillus pleuropneumoniae in probele biologice prelevate de la suine este disponibil in ...
more infohttp://www.pasteur.ro/noutati/un-nou-test-pcr-pentru-detectia-actinobacillus-pleuropneumoniae-in-probele-biologice-prelevate-de-la-suine-este-disponibil-in-cadrul-centrului-de-diagnostic-pasteur-romania/
BacMap  BacMap
Actinobacillus pleuropneumoniae, a Gram-negative, non-motile, facultatively anaerobic Pasteurellaceae, causes porcine ... short distances by droplet infection and only survives outside the pig for a few days.Actinobacillus pleuropneumoniae strain ... of apxICABD in strain JL03 confirms that it has the moderate toxicity specific to serotype 3.Actinobacillus pleuropneumoniae ...
more infohttp://bacmap.wishartlab.com/organisms/721
The role of lipooligosaccharide phosphorylcholine in colonization and pathogenesis of Histophilus somni in cattle | Veterinary...  The role of lipooligosaccharide phosphorylcholine in colonization and pathogenesis of Histophilus somni in cattle | Veterinary...
LPS has also been shown to contribute to the adherence of Actinobacillus pleuropneumoniae to host cells [44]. However, later ... Adherence of Actinobacillus pleuropneumoniae to primary cultures of porcine lung epithelial cells. Vet Microbiol. 2003, 93: 133 ... Role of lipopolysaccharides in adherence of Actinobacillus pleuropneumoniae to porcine tracheal rings. Infect Immun. 1990, 58: ... Haemophilus, Actinobacillus, and Pasteurella. edn. Edited by: Donachie W, Lainson FA, Hodgson JC. 1995, Plenum, New York, ...
more infohttps://0-veterinaryresearch-biomedcentral-com.brum.beds.ac.uk/articles/10.1186/1297-9716-43-49
Actinobacillus pleuropneumoniae serotype 5b (strain L20)  Actinobacillus pleuropneumoniae serotype 5b (strain L20)
Actinobacillus pleuropneumoniae L20. ›Actinobacillus pleuropneumoniae serovar 5b str. L20. ›Actinobacillus pleuropneumoniae ... Actinobacillus. › Actinobacillus pleuropneumoniae. › Actinobacillus pleuropneumoniae serovar 5. Strains i. › L20. See also. › ... Actinobacillus pleuropneumoniae serotype 5b (strain L20). Taxonomy navigation. › Actinobacillus pleuropneumoniae serovar 5 ...
more infohttps://www.uniprot.org/taxonomy/416269
Actinobacillus pleuropneumoniae (Shope) Pohl et al. ATCC ® 27088D  Actinobacillus pleuropneumoniae (Shope) Pohl et al. ATCC ® 27088D
Genomic DNA from Actinobacillus pleuropneumoniae 4074 TypeStrain=True Application: ... Actinobacillus pleuropneumoniae (Shope) Pohl et al. (ATCC® 27088D-5™) Strain Designations: Genomic DNA from Actinobacillus ... Genomic DNA from Actinobacillus pleuropneumoniae 4074 [ATCC® 27088™] Biosafety Level 1 Biosafety classification is based on U.S ... Nucleotide (GenBank) : AACK01000000 Actinobacillus pleuropneumoniae serovar 1 str. 4074, whole genome shotgun sequencing ...
more infohttps://www.atcc.org/en/Products/Cells_and_Microorganisms/Bacteria/Bacterial_Nucleic_Acids/27088D-5.aspx?p=1&rel=%7B0%7D
Comparative Genomic Characterization of Actinobacillus pleuropneumoniae | Journal of Bacteriology  Comparative Genomic Characterization of Actinobacillus pleuropneumoniae | Journal of Bacteriology
The Gram-negative bacterium Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumoniae, a ... Comparative Genomic Characterization of Actinobacillus pleuropneumoniae Zhuofei Xu, Xiabing Chen, Lu Li, Tingting Li, Shengyue ... Comparative Genomic Characterization of Actinobacillus pleuropneumoniae Zhuofei Xu, Xiabing Chen, Lu Li, Tingting Li, Shengyue ... Comparative Genomic Characterization of Actinobacillus pleuropneumoniae Zhuofei Xu, Xiabing Chen, Lu Li, Tingting Li, Shengyue ...
more infohttps://jb.asm.org/content/192/21/5625?ijkey=87845b60857552371511a9b50246567e0efe7e9f&keytype2=tf_ipsecsha
  • Taken together, TolC1 is required for biofilm formation and is a part of the MDR machinery of both planktonic and biofilm cells, which could supplement therapeutic strategies for resistant bacteria and biofilm-related infections of A. pleuropneumoniae clinical isolate SC1516. (frontiersin.org)
  • In particular, little is known regarding how A. pleuropneumoniae confers resistance against bactericidal substances for survival and spread within the host. (frontiersin.org)
more