Actinobacillus pleuropneumoniae: A species of gram-negative, facultatively anaerobic coccobacillus-shaped bacteria that has been isolated from pneumonic lesions and blood. It produces pneumonia with accompanying fibrinous pleuritis in swine.Actinobacillus Infections: Infections with bacteria of the genus ACTINOBACILLUS.Actinobacillus: A genus of PASTEURELLACEAE described as gram-negative, nonsporeforming, nonmotile, facultative anaerobes. Most members are found both as pathogens and commensal organisms in the respiratory, alimentary, and genital tracts of animals.Pleuropneumonia: Inflammation of the lung parenchyma that is associated with PLEURISY, inflammation of the PLEURA.Swine Diseases: Diseases of domestic swine and of the wild boar of the genus Sus.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Aggregatibacter actinomycetemcomitans: A species of Gram-negative, facultatively anaerobic spherical or rod-shaped bacteria indigenous to dental surfaces. It is associated with PERIODONTITIS; BACTERIAL ENDOCARDITIS; and ACTINOMYCOSIS.Haemophilus: A genus of PASTEURELLACEAE that consists of several species occurring in animals and humans. Its organisms are described as gram-negative, facultatively anaerobic, coccobacillus or rod-shaped, and nonmotile.Actinobacillus suis: A species of gram-negative bacteria in the genus ACTINOBACILLUS. It is mainly a pathogen of PIGS, but also can infect HORSES.Hemolysin Proteins: Proteins from BACTERIA and FUNGI that are soluble enough to be secreted to target ERYTHROCYTES and insert into the membrane to form beta-barrel pores. Biosynthesis may be regulated by HEMOLYSIN FACTORS.Serotyping: Process of determining and distinguishing species of bacteria or viruses based on antigens they share.Transferrin-Binding Proteins: A class of carrier proteins that bind to TRANSFERRIN. Many strains of pathogenic bacteria utilize transferrin-binding proteins to acquire their supply of iron from serum.Cytotoxins: Substances that are toxic to cells; they may be involved in immunity or may be contained in venoms. These are distinguished from CYTOSTATIC AGENTS in degree of effect. Some of them are used as CYTOTOXIC ANTIBIOTICS. The mechanism of action of many of these are as ALKYLATING AGENTS or MITOSIS MODULATORS.Pasteurella multocida: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria normally found in the flora of the mouth and respiratory tract of animals and birds. It causes shipping fever (see PASTEURELLOSIS, PNEUMONIC); HEMORRHAGIC BACTEREMIA; and intestinal disease in animals. In humans, disease usually arises from a wound infection following a bite or scratch from domesticated animals.Pleuropneumonia, Contagious: A pleuropneumonia of cattle and goats caused by species of MYCOPLASMA.Aspartate Ammonia-Lyase: An enzyme that catalyzes the conversion of aspartic acid to ammonia and fumaric acid in plants and some microorganisms. EC 4.3.1.1.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Pasteurellaceae: A family of coccoid to rod-shaped nonsporeforming, gram-negative, nonmotile, facultatively anaerobic bacteria that includes the genera ACTINOBACILLUS; HAEMOPHILUS; MANNHEIMIA; and PASTEURELLA.Bacterial Proteins: Proteins found in any species of bacterium.Transferrin-Binding Protein B: A subtype of bacterial transferrin-binding protein found in bacteria. It forms a cell surface receptor complex with TRANSFERRIN-BINDING PROTEIN A.Bacterial Vaccines: Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.Pasteurella: The oldest recognized genus of the family PASTEURELLACEAE. It consists of several species. Its organisms occur most frequently as coccobacillus or rod-shaped and are gram-negative, nonmotile, facultative anaerobes. Species of this genus are found in both animals and humans.Palatine Tonsil: A round-to-oval mass of lymphoid tissue embedded in the lateral wall of the PHARYNX. There is one on each side of the oropharynx in the fauces between the anterior and posterior pillars of the SOFT PALATE.Tylosin: Macrolide antibiotic obtained from cultures of Streptomyces fradiae. The drug is effective against many microorganisms in animals but not in humans.Actinobacillus equuli: A genus of gram-negative bacteria in the genus ACTINOBACILLUS, which is pathogenic for HORSES and PIGS.Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Genes, Bacterial: The functional hereditary units of BACTERIA.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Bacterial Capsules: An envelope of loose gel surrounding a bacterial cell which is associated with the virulence of pathogenic bacteria. Some capsules have a well-defined border, whereas others form a slime layer that trails off into the medium. Most capsules consist of relatively simple polysaccharides but there are some bacteria whose capsules are made of polypeptides.Iron-Binding Proteins: Proteins that specifically bind to IRON.Bacterial Toxins: Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.Lung: Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.Bacterial Outer Membrane Proteins: Proteins isolated from the outer membrane of Gram-negative bacteria.Sulfite Reductase (Ferredoxin): A FERREDOXIN-dependent oxidoreductase that is primarily found in PLANTS where it plays an important role in the assimilation of SULFUR atoms for the production of CYSTEINE and METHIONINE.Polysaccharides, Bacterial: Polysaccharides found in bacteria and in capsules thereof.Haemophilus paragallinarum: A species of gram-negative bacteria in the genus HAEMOPHILUS, causing respiratory tract disease in CHICKENS known as infectious coryza.Pasteurella Infections: Infections with bacteria of the genus PASTEURELLA.Mannheimia haemolytica: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria normally commensal in the flora of CATTLE and SHEEP. But under conditions of physical or PHYSIOLOGICAL STRESS, it can cause MASTITIS in sheep and SHIPPING FEVER or ENZOOTIC CALF PNEUMONIA in cattle. Its former name was Pasteurella haemolytica.Fibroma Virus, Rabbit: A species of LEPORIPOXVIRUS causing subcutaneous localized swellings in rabbits, usually on the feet.Phytoplasma: A genus of minute bacteria in the family ACHOLEPLASMATACEAE that inhabit phloem sieve elements of infected PLANTS and cause symptoms such as yellowing, phyllody, and witches' brooms. Organisms lack a CELL WALL and thus are similar to MYCOPLASMA in animals. They are transmitted by over 100 species of INSECTS especially leafhoppers, planthoppers, and PSYLLIDS.Hydrangea: A plant genus of the family HYDRANGEACEAE. Members contain hydrangenol, thunberginols, hydramacrosides A and B, and secoiridoid glucosides.Biofilms: Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.Thiamphenicol: A methylsulfonyl analog of CHLORAMPHENICOL. It is an antibiotic and immunosuppressive agent.Drugs, Generic: Drugs whose drug name is not protected by a trademark. They may be manufactured by several companies.Immunity, Maternally-Acquired: Resistance to a disease-causing agent induced by the introduction of maternal immunity into the fetus by transplacental transfer or into the neonate through colostrum and milk.Immunomagnetic Separation: A cell-separation technique where magnetizable microspheres or beads are first coated with monoclonal antibody, allowed to search and bind to target cells, and are then selectively removed when passed through a magnetic field. Among other applications, the technique is commonly used to remove tumor cells from the marrow (BONE MARROW PURGING) of patients who are to undergo autologous bone marrow transplantation.C-Reactive Protein: A plasma protein that circulates in increased amounts during inflammation and after tissue damage.Biological Markers: Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.Abattoirs: Places where animals are slaughtered and dressed for market.HungaryHemagglutination Tests: Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)
Pulmonary lesions in guinea pigs experimentally infected with Actinobacillus pleuropneumoniae (A.p.) serovar 1. (1/288)
Pathological studies were carried out on the lungs of guinea pigs intratracheally inoculated with 4.6 x 10(6-8) colony forming units (CFU)/head of Actinobacillus pleuropneumoniae serovar 1. All animals in the highest dose group died within 24 hr post inoculation (hpi) and showed pulmonary lesions being hemorrhagic in nature while all animals in the lowest dose group were killed as scheduled at 11 days post inoculation (dpi) and showed only hyperplasia of peribronchial lymphoid tissues. In the middle dose group, two died within 24 hpi, two died at 9 dpi, and the remaining one was killed at 11 dpi. Two guinea pigs which died at 9 dpi showed fibrinonecrotic pleuropneumonia which is the most characteristic acute pulmonary lesion in swine, and has not yet been reproduced in laboratory animals up to the present time. This suggests that guinea pigs may be a useful laboratory animal for studying the pathogenesis of Actinobacillus pleuropneumoniae infection in swine. (+info)Vaccination and protection of pigs against pleuropneumonia with a vaccine strain of Actinobacillus pleuropneumoniae produced by site-specific mutagenesis of the ApxII operon. (2/288)
The production of toxin (Apx)-neutralizing antibodies during infection plays a major role in the induction of protective immunity to Actinobacillus pleuropneumoniae reinfection. In the present study, the gene encoding the ApxII-activating protein, apxIIC, was insertionally inactivated on the chromosome of a serovar 7 strain, HS93. Expression of the structural toxin, ApxIIA, and of the two genes required for its secretion, apxIB and apxID, still occurs in this strain. The resulting mutant strain, HS93C- Ampr, was found to secrete the unactivated toxin. Pigs vaccinated with live HS93C- Ampr via the intranasal route were protected against a cross-serovar challenge with a virulent serovar 1 strain of A. pleuropneumoniae. This is the first reported vaccine strain of A. pleuropneumoniae which can be delivered live to pigs and offers cross-serovar protection against porcine pleuropneumonia. (+info)Molecular cloning and sequencing of the aroA gene from Actinobacillus pleuropneumoniae and its use in a PCR assay for rapid identification. (3/288)
The gene (aroA) of Actinobacillus pleuropneumoniae, serotype 2, encoding 5-enolpyruvylshikimate-3-phosphate synthase was cloned by complementation of the aroA mutation in Escherichia coli K-12 strain AB2829, and the nucleotide sequence was determined. A pair of primers from the 5' and 3' termini were selected to be the basis for development of a specific PCR assay. A DNA fragment of 1,025 bp was amplified from lysed A. pleuropneumoniae serotypes 1 to 12 of biovar 1 or from isolated DNA. No PCR products were detected when chromosomal DNAs from other genera were used as target DNAs; however, a 1,025-bp DNA fragment was amplified when Actinobacillus equuli chromosomal DNA was used as a target, which could be easily differentiated by its NAD independence. The PCR assay developed was very sensitive, with lower detection limits of 12 CFU with A. pleuropneumoniae cells and 0.8 pg with extracted DNA. Specificity and sensitivity make this PCR assay a useful method for the rapid identification and diagnosis of A. pleuropneumoniae infections. (+info)Actinobacillus pleuropneumoniae osteomyelitis in pigs demonstrated by fluorescent in situ hybridization. (4/288)
Necrotizing osteomyelitis and fibrinopurulent arthritis with isolation of Actinobacillus pleuropneumoniae serotype 2 is reported in two pigs from a herd with lameness and mild coughing problems among 8 to 12-week-old pigs. Application of fluorescent in situ hybridization targeting 16S ribosomal RNA of A. pleuropneumoniae in formalin-fixed tissue was performed to verify the association of A. pleuropneumoniae with the bone and joint lesions. By in situ hybridization A. pleuropneumoniae was demonstrated as multiple microcolonies or single cells dispersed in focal fibrinonecrotizing pleuropneumonia, in joints with arthritis, and in bone necroses including lysis of growth plate and suppurative inflammation in the adjacent trabecular metaphysis, thus demonstrating that well-known infections manifest new, unusual lesions. (+info)A novel enzyme-linked immunosorbent assay using the recombinant Actinobacillus pleuropneumoniae ApxII antigen for diagnosis of pleuropneumonia in pig herds. (5/288)
For the surveillance of pig herds infected with porcine pleuropneumonia, an enzyme-linked immunosorbent assay (ELISA) using the recombinant Actinobacillus pleuropneumoniae ApxII protein as species- but not serotype-specific antigen was developed. Using this ELISA, 243 of 400 animals from 22 A. pleuropneumoniae-infected herds were classified as seropositive. (+info)First chromosomal restriction map of Actinobacillus pleuropneumoniae and localization of putative virulence-associated genes. (6/288)
Combined physical and genetic maps of the genomes of Actinobacillus pleuropneumoniae AP76 (serotype 7 clinical isolate) and of A. pleuropneumoniae ATCC 27088 (serotype 1 reference strain) were constructed by using the restriction endonucleases ApaI, AscI, NotI, and SalI. The chromosome sizes as determined by the addition of estimated fragment sizes were 2.4 Mbp, and both maps had a resolution of approximately 100 kbp. The linkages between the ApaI, AscI, NotI, and SalI fragments and their relative positions were determined by (i) fragment excision and redigestion and (ii) partial digests of defined fragments and Southern blot using end-standing probes. The single SalI site within the chromosome of strain A. pleuropneumoniae AP76 was defined as position 1 of the map; for the map of A. pleuropneumoniae ATCC 27088, the corresponding SalI site was chosen. Putative virulence-associated genes (apx, omlA, sodA, tbpBA, ureC, and a repeat element) and housekeeping genes (glyA, metJ, recA, and rhoAP) were positioned on the physical maps and located on the ApaI and NotI fragments of A. pleuropneumoniae serotype reference strains. (+info)Detection of antibodies against Actinobacillus pleuropneumoniae serotypes 1, 2, 5 and 7 using the immunohistochemical staining. (7/288)
Whole cells of Actinobacillus pleuropneumoniae (A. pleuropneumoniae) serotype 1, 2, 5 or 7 attached to fibrins were fixed in 10% neutral buffered formalin and embedded in paraffin. The sections on a slide glass were stained by the avidin-biotin complex immunoperoxidase (ABC) method. Test sera were applied to sections as primary antibodies. The serum antibodies against A.pleuropneumoniae (serotypes 1, 2, 5 and 7) were measured by the ABC method and complement fixation (CF) test. There was good correlation between the ABC and CF tests. The present results indicate that the immunohistochemical staining is as useful as the CF test for the detection and quantification of antibody in swine sera. (+info)Characterization of apxIVA, a new RTX determinant of Actinobacillus pleuropneumoniae. (8/288)
A fourth type of RTX determinant was identified in Actinobacillus pleuropneumoniae and was designated apxIVA. When expressed in Escherichia coli, recombinant ApxIVA showed a weak haemolytic activity and co-haemolytic synergy with the sphingomyelinase (beta-toxin) of Staphylococcus aureus. These activities required the presence of an additional gene, ORF1, that is located immediately upstream of apxIVA. The apxIVA gene product could not be detected in A. pleuropneumoniae cultures grown under various conditions in vitro; however, pigs experimentally infected with A. pleuropneumoniae serotypes 1, 5 and 7 started to produce antibodies that reacted with recombinant ApxIVA 14 d post-infection, indicating that apxIVA is expressed in vivo. In addition, sera from pigs naturally and experimentally infected with any of the serotypes all reacted with recombinant ApxIVA. The apxIVA gene from the serotype 1 A. pleuropneumoniae type strain Shope 4074T encodes a protein with a predicted molecular mass of 202 kDa which has typical features of RTX proteins including hydrophobic domains in the N-terminal half and 24 glycine-rich nonapeptides in the C-terminal half that bind Ca2+. The glycine-rich nonapeptides are arranged in a modular structure and there is some variability in the number of modules in the ApxIVA proteins of different serotypes of A. pleuropneumoniae. The deduced amino acid sequences of the ApxIVA proteins have significant similarity with the Neisseria meningitidis iron-regulated RTX proteins FrpA and FrpC, and to a much lesser extent with other RTX proteins. The apxIVA gene could be detected in all A. pleuropneumoniae serotypes and seems to be species-specific. Although the precise role of this new RTX determinant in pathogenesis of porcine pleuropneumonia needs to be determined, apxIVA is the first in vivo induced toxin gene that has been described in A. pleuropneumoniae. (+info)"Development of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae infection". Vaccine. 24 (49): 7226-32. doi: ... Actinobacillus pleuropneumonia and Salmonella infections in pigs. Vaccine development has several trends: Until recently,[when ...
"Poly-N-acetylglucosamine mediates biofilm formation and antibiotic resistance in Actinobacillus pleuropneumoniae". Microb ... Kaplan JB, Ragunath C, Ramasubbu N, Fine DH (August 2003). "Detachment of Actinobacillus actinomycetemcomitans biofilm cells by ... Kaplan JB, Meyenhofer MF, Fine DH (2003). "Biofilm growth and detachment of Actinobacillus actinomycetemcomitans". J. Bacteriol ... a biofilm-releasing glycoside hydrolase from the periodontopathogen Actinobacillus actinomycetemcomitans". J. Mol. Biol. 349 (3 ...
Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae are pathogens that cause respiratory disease in swine. Haemophilus ... coli TOP10 strain Campylobacter jejuni Mycoplasma hyopneumoniae Haemophilus influenzae Actinobacillus pleuropneumoniae ...
Clade II, encompassing Actinobacillus pleuropneumoniae, Actinobacillus minor, Haemophilus ducreyi, Mannheimia haemolytica and ... Two major clades are formed within this Pasteurellales: Clade I, encompassing Aggregatibacter, Pasteurella, Actinobacillus ...
... reducing pig deaths and disease from the bacteria Actinobacillus pleuropneumoniae. The Molecular Plant Breeding CRC developed ...
Actinobacillus pleuropneumoniae, and Streptococcus suis. Mastitis-metritis-agalactia syndrome involved with E. coli, ...
... while the other includes Actinobacillus minor, Actinobacillus pleuropneumoniae, Haemophilus ducreyi, Haemophilus parasuis, and ... The distribution of CSIs corresponds to sensu stricto clades of "true" Actinobacillus, Haemophilus, and Pasteurella species, ... and Actinobacillus species. Comparative analyses of Pasteurellaceae genomes have identified large numbers (>20) of conserved ... Actinobacillus, Haemophilus, and Pasteurella. These genera demonstrate extensive polyphyly across the family, however, CSIs ...
Actinobacillus equuli MeSH B03.440.450.009.580 --- Actinobacillus pleuropneumoniae MeSH B03.440.450.009.675 --- Actinobacillus ... Actinobacillus equuli MeSH B03.660.250.550.050.580 --- Actinobacillus pleuropneumoniae MeSH B03.660.250.550.050.675 --- ... Actinobacillus seminis MeSH B03.660.250.550.050.700 --- Actinobacillus suis MeSH B03.660.250.550.290 --- Haemophilus MeSH ... Actinobacillus MeSH B03.660.250.550.050.050 --- Actinobacillus actinomycetemcomitans MeSH B03.660.250.550.050.200 --- ...
2008). "Actinobacillus pleuropneumoniae". Pasteurellaceae: Biology, Genomics and Molecular Aspects. Caister Academic Press. ... Actinobacillus (Pasteurella) ureae and A. hominis occur in the respiratory tracts of healthy humans and may be involved in the ... Actinobacillus is a genus of Gram-negative, nonmotile and nonspore-forming, oval to rod-shaped bacteria occurring as parasites ... ISBN 978-1-904455-24-0. Nørskov-Lauritsen N, Kilian M (September 2006). "Reclassification of Actinobacillus ...
Streptococcus suis and Actinobacillus pleuropneumoniae. Antibiotics such as ceftiofur, gentamicin and trimethoprim/sulfadiazine ... Actinobacillus suis is a beta-haemolytic, Gram-negative bacterium of the Pasteurellaceae family. The bacterium has many strains ... Type strain of Actinobacillus suis at BacDive - the Bacterial Diversity Metadatabase. ... Actinobacillus suis, reviewed and published by Wikivet at http://en.wikivet.net/Actinobacillus_suis accessed 07/10/2011. ...
... (previously Haemophilus pleuropneumoniae), is a Gram-negative, facultative anaerobic, ... Gillaspy, A. "Actinobacillus pleuropneumoniae". Laboratory for Molecular Biology and Cytometry Research-University of Oklahoma ... "Actinobacillus pleuropneumoniae". Iowa State University College of Veterinary Medicine. Retrieved 20 April 2013. [permanent ... Brownfield, B. "Actinobacillus pleuropneumoniae in swine". Purdue University-Animal Disease Diagnostic Laboratory. Retrieved 20 ...
"Development of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae infection". Vaccine. 24 (49-50): 7226-37. doi: ... Actinobacillus pleuropneumonia[111] and Salmonella infections in pigs.[112] ...
Isolation of Actinobacillus pleuropneumoniae serotype 2 by immunomagnetic separation. Publication: Research - peer-review › ... In Denmark porcine pleuropneumonia is most frequently caused by Actinobacillus pleuropneumoniae serotype 2 (60%). Isolation of ... By using IMS A. pleuropneumoniae serotype 2 could be reisolated from the tonsils of three pigs. The LMS method represents a ... From 12 pigs inoculated with an aerosol of A. pleuropneumoniae serotype 2 the bacterium could not be detected from the nasal ...
title = "Actinobacillus pleuropneumoniae serotypes in Hungary",. abstract = "A total of 255 Actinobacillus pleuropneumoniae ... Actinobacillus pleuropneumoniae serotypes in Hungary. / Sárközi, Rita; Makrai, L.; Fodor, L.. In: Acta Veterinaria Hungarica, ... Sárközi, Rita ; Makrai, L. ; Fodor, L. / Actinobacillus pleuropneumoniae serotypes in Hungary. In: Acta Veterinaria Hungarica. ... Sárközi, R., Makrai, L., & Fodor, L. (2018). Actinobacillus pleuropneumoniae serotypes in Hungary. Acta Veterinaria Hungarica, ...
Actinobacillus pleuropneumoniae comb. nov.) on the basis of phenotypic and deoxyribonucleic acid relatedness. Int J Syst ... Reclassification of equine isolates previously reported as Actinobacillus equuli, variants of A. equuli, Actinobacillus suis or ... Pohl, S., Bertschinger, H. U., Frederiksen, W. & Mannheim, W. ( 1983 ). Transfer of Haemophilus pleuropneumoniae and the ... Christensen, H. & Bisgaard, M. ( 2004 ). Revised definition of Actinobacillus sensu stricto isolated from animals: a review ...
Actinobacillus pleuropneumoniae (previously Haemophilus pleuropneumoniae), is a Gram-negative, facultative anaerobic, ... Gillaspy, A. "Actinobacillus pleuropneumoniae". Laboratory for Molecular Biology and Cytometry Research-University of Oklahoma ... "Actinobacillus pleuropneumoniae". Iowa State University College of Veterinary Medicine. Retrieved 20 April 2013. [permanent ... Brownfield, B. "Actinobacillus pleuropneumoniae in swine". Purdue University-Animal Disease Diagnostic Laboratory. Retrieved 20 ...
Shipping (test code: xtrnc) and handling (test code: xhand) fees are also applicable on each submission. External test price is subject to change. ...
Actinobacillus pleuropneumoniae L20. ›Actinobacillus pleuropneumoniae serovar 5b str. L20. ›Actinobacillus pleuropneumoniae ... Actinobacillus. › Actinobacillus pleuropneumoniae. › Actinobacillus pleuropneumoniae serovar 5. Strains i. › L20. See also. › ... Actinobacillus pleuropneumoniae serotype 5b (strain L20). Taxonomy navigation. › Actinobacillus pleuropneumoniae serovar 5 ...
Characterization of apxIVA, a new RTX determinant of Actinobacillus pleuropneumoniae.. Schaller A1, Kuhn R, Kuhnert P, Nicolet ... A fourth type of RTX determinant was identified in Actinobacillus pleuropneumoniae and was designated apxIVA. When expressed in ... The apxIVA gene could be detected in all A. pleuropneumoniae serotypes and seems to be species-specific. Although the precise ... The apxIVA gene from the serotype 1 A. pleuropneumoniae type strain Shope 4074T encodes a protein with a predicted molecular ...
Genomic DNA from Actinobacillus pleuropneumoniae 4074 TypeStrain=True Application: ... Actinobacillus pleuropneumoniae (Shope) Pohl et al. (ATCC® 27088D-5™) Strain Designations: Genomic DNA from Actinobacillus ... Genomic DNA from Actinobacillus pleuropneumoniae 4074 [ATCC® 27088™] Biosafety Level 1 Biosafety classification is based on U.S ... Nucleotide (GenBank) : AACK01000000 Actinobacillus pleuropneumoniae serovar 1 str. 4074, whole genome shotgun sequencing ...
Actinobacillus pleuropneumoniae ATCC ® 33590™ Designation: Femo TypeStrain=True Application: Characterization Respiratory ... Actinobacillus pleuropneumoniae (Shope) Pohl et al. (ATCC® 33590™) Strain Designations: Femo / Type Strain: yes / Biosafety ... Analysis of Actinobacillus pleuropneumoniae and related organisms by DNA-DNA hybridization and restriction endonuclease ... Biochemical and serological characterization of Haemophilus pleuropneumoniae (Matthews and Pattison 1961) Shope 1964 and ...
The Gram-negative bacterium Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumoniae, a ... Comparative Genomic Characterization of Actinobacillus pleuropneumoniae Zhuofei Xu, Xiabing Chen, Lu Li, Tingting Li, Shengyue ... Comparative Genomic Characterization of Actinobacillus pleuropneumoniae Zhuofei Xu, Xiabing Chen, Lu Li, Tingting Li, Shengyue ... Comparative Genomic Characterization of Actinobacillus pleuropneumoniae Zhuofei Xu, Xiabing Chen, Lu Li, Tingting Li, Shengyue ...
Two Actinobacillus pleuropneumoniae serotype 7 antigens were expressed in Escherichia coli and tested for their protective ... Immunization of pigs against Actinobacillus pleuropneumoniae with two recombinant protein preparations.. Rossi-Campos A1, ... Upon challenge with an A. pleuropneumoniae serotype 7 strain, all immunized groups were less affected by the disease and showed ... Protection was serotype-specific since no cross-protection was detected following challenge with an A. pleuropneumoniae ...
Actinobacillus pleuropneumoniae serovar 10 str. D13039. Actinobacillus pleuropneumoniae serovar 4 str. M62. Actinobacillus ... Actinobacillus pleuropneumoniae serovar 10 str. D13039. Actinobacillus pleuropneumoniae serovar 4 str. M62. Actinobacillus ... Actinobacillus pleuropneumoniae serovar 11 str. 56153. Actinobacillus pleuropneumoniae serovar 13 str. N273. Actinobacillus ... Actinobacillus pleuropneumoniae serovar 11 str. 56153. Actinobacillus pleuropneumoniae serovar 13 str. N273. Actinobacillus ...
... an antigenic membrane protein from Actinobacillus pleuropnemoniae homology to ABC transporters; amino acid sequence in first ... Actinobacillus pleuropneumoniae ApaA protein. Subscribe to New Research on Actinobacillus pleuropneumoniae ApaA protein ... an antigenic membrane protein from Actinobacillus pleuropnemoniae homology to ABC transporters; amino acid sequence in first ...
DNA sequence analysis of the fragment showed that there are three genes designated afuA, afuB and afuC (Actinobacillus ferric ... The cloned afu locus of Actinobacillus pleuropneumoniae restored the ability of an Escherichia coli K-12 mutant (aroB) to grow ... Identification of a locus involved in the utilization of iron by Actinobacillus pleuropneumoniae FEMS Microbiol Lett. 1996 Sep ... The cloned afu locus of Actinobacillus pleuropneumoniae restored the ability of an Escherichia coli K-12 mutant (aroB) to grow ...
... pleuropneumoniae and divided the isolates of A. pleuropneumoniae into 20 clusters. Most of the serovars of A. pleuropneumoniae ... Actinobacillus pleuropneumoniae, p. 563-576. In B. E. Straw, J. J. Zimmerman, S. DAllaire, and D. J. Taylor (ed.), Diseases of ... An Actinobacillus pleuropneumoniae PCR typing system based on the apx and omlA genes-evaluation of isolates from lungs and ... A. pleuropneumoniae is genetically closely affiliated with Actinobacillus lignieresii (6, 25), a bacterium commonly found in ...
The IDEXX APP-ApxIV Ab Test is an enzyme immunoassay for the detection of antibodies against Actinobacillus pleuropneumoniae ( ... Actinobacillus pleuropneumoniae (APP). Swine pleuropneumonia caused by Actinobacillus pleuropneumoniae (APP) is a highly ... Actinobacillus pleuropneumoniae (APP). The IDEXX APP-ApxIV Ab Test is an enzyme immunoassay for the detection of antibodies ... against Actinobacillus pleuropneumoniae (APP), the causative pathogen for swine pleuropneumonia, in serum and plasma of swine ...
In this study, we identified two TolC-like proteins, TolC1 and TolC2, in A. pleuropneumoniae. Deletion of tolC1, but not tolC2 ... In this study, we identified two TolC-like proteins, TolC1 and TolC2, in A. pleuropneumoniae. Deletion of tolC1, but not tolC2 ... pleuropneumoniae. Taken together, TolC1 is required for biofilm formation and is a part of the multidrug resistance machinery ... pleuropneumoniae. Taken together, TolC1 is required for biofilm formation and is a part of the multidrug resistance machinery ...
Actinobacillus pleuropneumoniae and Pasteurella multocida. Pharmacokinetic data were pooled for two bioequivalent products, ... pleuropneumoniae). For daily doses at steady state, and 90% TAR bacteriostatic and bactericidal actions, dosages of 6.2 and 9.6 ... pleuropneumoniae. Using these PK and PD data, together with literature MIC distributions, doses for each pathogen were ... mg/kg (P. multocida) and 18.2 and 35.2 mg/kg (A. pleuropneumoniae) were required. PK/PD integration and modelling approaches to ...
Lipopolysaccharides of Actinobacillus pleuropneumoniae bind pig hemoglobin.. M Bélanger, C Bégin, M Jacques ... Lipopolysaccharides of Actinobacillus pleuropneumoniae bind pig hemoglobin. Message Subject (Your Name) has forwarded a page to ... A previous study indicated that lipopolysaccharides (LPS) extracted from Actinobacillus pleuropneumoniae bind two low-molecular ... Our results indicate that LPS of all A. pleuropneumoniae isolates tested bind pig hemoglobin and that lipid A is involved in ...
MUNOZ, D; QUEZADA, M y RUIZ, A. Serological behaviour study of Actinobacillus pleuropneumoniae (App) in commercial swine herds ... Palabras clave : Actinobacillus pleuropneumoniae; seroprevalence; seroconversion. · resumen en Español · texto en Español · ...
An Actinobacillus pleuropneumoniae (APP) challenge model was used to evaluate the duration of antibiotic effectiveness of ... In the U.S., approval has been granted for treatment of SRD associated with Actinobacillus pleuropneumoniae, Pasteurella ... Draxxin® (tulathromycin) Injectable Solution: Duration of Effectiveness in an Actinobacillus Pleuropneumoniae Challenge Model. ... weight given at different times prior to intranasal challenge with a highly virulent strain of Actinobacillus pleuropneumoniae ...
Keywords: Actinobacillus pleuropneumoniae, enzyme-linked immunosorbent assay, polymerase chain reaction. Cite as: Broes A, ... Serological testing is widely used to monitor swine herds for Actinobacillus pleuropneumoniae (APP). Several serological tests ... Dealing with unexpected Actinobacillus pleuropneumoniae serological results. Andr? Broes, DVM, PhD, Diplomate ECPHM; Guy-Pierre ... Martineau GP, Gottschalk M. Dealing with unexpected Actinobacillus pleuropneumoniae serological results. J Swine Health Prod ...
Actinobacillus pleuropneumoniae infections in pigs: the role of virulence factors in pathogenesis and protection. Vet. ... D) Two wild-type strains of A. pleuropneumoniae and E. coli DH5α expressing the A. pleuropneumoniae pgaCD genes on a plasmid ( ... Expression of A. pleuropneumoniae pgaCD in E. coli.Genomic DNA isolated from A. pleuropneumoniae strain IA1 was amplified by ... Expression and purification of A. pleuropneumoniae dispersin B.Genomic DNA isolated from A. pleuropneumoniae strain IA1 was ...
Actinobacillus pleuropneumoniae (APP) was first isolated from pig lung in 1957 and initially described as Haemophilus-like ... Actinobacillus pleuropneumoniae is one of the most important bacterial pathogens, which causes PCP (Li et al., 2016). For ... 2008). Efficacy of a subunit vaccine against Actinobacillus pleuropneumoniae in an endemically infected swine herd. J. Swine ... 2014). Transmission of Actinobacillus pleuropneumoniae among weaned piglets on endemically infected farms. Prev. Vet. Med. 117 ...
MICs and MBCs of Tiamulin against Actinobacillus pleuropneumoniae (App) in Broth and 100 Per Cent Serum. Disease and welfare ... against Actinobacillus pleuropneumoniae (App), using standardised MIC determinations, have been unsuccessful and led to ... in plasma with tiamulin MICs against Actinobacillus pleuropneumoniae (App) have proven ineffective, despite good clinical and ...
SerotypesSwineStrainsPathogenAgent of porcine pleuropneumoniaStrainsPasteurellaceaeBacteriaEscherichiaPneumonia causedProteinInfectionCharacterizationSerologicalLignieresiiExperimentally infectedSerovar 5bEtiologic agent of porcineApxIOrganismActinomycetemcomitansGeneCausative agentLesionsJL03LungProteinsBiofilm formationVirulenceVaccinePigletsSusceptibilityGenesBacterialShopeAntigensAntigenicOuter membraneAP76PlasmidsContagiousAerosolPathogens
- Fourteen Hungarian A. pleuropneumoniae isolates from the culture collection of the Department of Microbiology and Infectious Diseases, isolated before 2012, were also included in the evaluation (one each from 12 herds and two each from two herds, where two serotypes occurred). (elsevier.com)
- A total of 255 Actinobacillus pleuropneumoniae isolates were collected from 634 lung samples representing 70 swine herds in Hungary between January 2012 and June 2016. (elsevier.com)
- Out of the altogether 91 A. pleuropneumoniae strains 72 strains belonged to biotype I and 19 strains could be allocated to biotype II. (elsevier.com)
- As a facultative anaerobic pathogen, A. pleuropneumoniae may need CO2 to grow. (wikipedia.org)
- Our findings shed more light on genomic features associated with serovar diversity of A. pleuropneumoniae and provide broader insight into both pathogenesis research and clinical/epidemiological application against the severe disease caused by this swine pathogen. (asm.org)
- Actinobacillus pleuropneumoniae is a gram-negative, encapsulated respiratory pathogen of swine and the causative agent of porcine pleuropneumonia ( 14 ). (asm.org)
- The IDEXX APP-ApxIV Ab Test is an enzyme immunoassay for the detection of antibodies against Actinobacillus pleuropneumoniae (APP), the causative pathogen for swine pleuropneumonia, in serum and plasma of swine independently of the APP serotype. (idexx.com)
- In this report, we present genetic and biochemical evidence that PGA is also a major matrix component of biofilms produced by the human periodontopathogen Actinobacillus actinomycetemcomitans and the porcine respiratory pathogen Actinobacillus pleuropneumoniae . (asm.org)
- Despite this, scattered case reports of arthritis, osteomyelitis, hepatitis, meningitis or nephritis exist, in which A. pleuropneumoniae remained the only detectable pathogen. (biomedcentral.com)
- A. pleuropneumoniae is distributed worldwide and considered a primary pathogen that can cause severe respiratory disease. (biomedcentral.com)
- Despite its non-invasive character there are several case reports about non-respiratory clinical diseases in which A. pleuropneumoniae was the only detectable pathogen. (biomedcentral.com)
- In this study, we report the 1.98 A resolution structure of TbpB from the porcine pathogen Actinobacillus pleuropneumoniae, providing insights into the mechanism of Tf binding and the role of TbpB. (rcsb.org)
- Actinobacillus pleurapneumoniae is the causative agent of porcine pleuropneumonia, a severe respiratory illness that it results in significant economic losses in the swine industry. (ufv.br)
- Actinobacillus pleuropneumoniae (A.pp.) is the causative agent of porcine pleuropneumonia leading to high economic losses in the pig industry. (beds.ac.uk)
- Actinobacillus pleuropneumoniae (APP), the etiologic agent of porcine pleuropneumonia, forms biofilms on biotic and abiotic surfaces. (umontreal.ca)
- In order to elucidate the species boundaries, six strains of the phylogenetically closely related species Actinobacillus lignieresii were also included. (asm.org)
- Previous studies have shown that strains within a given serovar of A. pleuropneumoniae are genetically very similar, underlining that A. pleuropneumoniae seems to have a clonal population structure ( 32 ). (asm.org)
- The corresponding regions of all 12 A. pleuropneumoniae reference strains of biovar 1 were sequenced. (semanticscholar.org)
- Out of the altogether 91 A. pleuropneumoniae strains 72 strains belonged to biotype I and 19 strains could be allocated to biotype II. (elsevier.com)
- Sixty-eight Actinobacillus pleuropneumoniae strains were isolated from porcine acute pleuropneumonia cases from different parts of Hungary between 2000 and 2014. (usda.gov)
- A total of 41 isolates were identified as A. pleuropneumoniae bio-type I and 27 strains as biotype II based on cultural, morphological and biochemical characteristics. (usda.gov)
- Twenty-nine field strains were correctly identified by the Biolog system as A. pleuropneumoniae, 36 strains as A. lignieresii, two strains as H. paraphrohaemolyticus and one strain as A. equuli after 24 h of incubation. (usda.gov)
- Among the six A. pleuropneumoniae reference strains the Biolog system identified one strain as A. pleuropneumoniae, four as A. lignieresii and one as H. paraphrohaemolyticus. (usda.gov)
- our data indicate that the efficacy of the Biolog system used here could be improved by including phenotypes of more A. pleuropneumoniae strains representing a wider geographical occurrence into the database. (usda.gov)
- Experimental Identification of Actinobacillus pleuropneumoniae Strains L20 and JL03 Heptosyltransferases, Evidence for a New Heptosyltransferase Signature Sequence. (recercat.cat)
- A pleuropneumoniae grows readily in the laboratory, but most strains require NAD, a growth factor, often supplied by other bacteria or by altered blood. (pigprogress.net)
- A. pleuropneumoniae is a nonmotile, Gram-negative, encapsulated coccobacillus bacterium found in the Pasteurellaceae family. (wikipedia.org)
- Actinobacillus pleuropneumoniae , a Gram-negative facultative anaerobic encapsulated coccobacillus, belongs to the Actinobacillus genus of the Pasteurellaceae family ( 19 ). (asm.org)
- Conclusions: This is the first description of mobilizable plasmids conferring trimethoprim resistance in A. pleuropneumoniae and, to our knowledge, the first report of dfrA14 in any member of the Pasteurellaceae. (st-andrews.ac.uk)
- Actinobacillus ( A. ) pleuropneumoniae , a Gram-negative bacterium belonging to the family Pasteurellaceae , is one of the most important respiratory tract pathogens in the pig industry. (biomedcentral.com)
- The complete nucleotide sequence of a 7.7kb mobilisable plasmid (pM3446F), isolated from a florfenicol resistant isolate of Actinobacillus pleuropneumoniae, showed extended similarity to plasmids found in other members of the Pasteurellaceae containing the floR gene as well as replication and mobilisation genes. (cdc.gov)
- Taken together, TolC1 is required for biofilm formation and is a part of the MDR machinery of both planktonic and biofilm cells, which could supplement therapeutic strategies for resistant bacteria and biofilm-related infections of A. pleuropneumoniae clinical isolate SC1516. (frontiersin.org)
- Actinobacillus is a genus of Gram-negative, nonmotile and nonspore-forming, oval to rod-shaped bacteria occurring as parasites or pathogens in mammals, birds, and reptiles. (wikipedia.org)
- The present invention pertains to a method to produce RTX-toxin Apxl by culturing Actinobacillus pleuropneumonica bacteria in a liquid culturing medium that supports growth of the bacteria, to which medium a calcium salt is added to form calcium ions in the medium, wherein the culturing medium contains borogluconate to form a calcium borogluconate complex in the medium. (patentsencyclopedia.com)
- 1. A method to produce RTX-toxin ApxI comprising culturing Actinobacillus pleuropneumoniae bacteria in a culturing medium that supports growth of the bacteria, to which medium a calcium salt is added to form calcium ions in the medium, characterised in that the culturing medium contains borogluconate to form a calcium borogluconate complex in the medium. (patentsencyclopedia.com)
- Two Actinobacillus pleuropneumoniae serotype 7 antigens were expressed in Escherichia coli and tested for their protective efficacy in an experimental pig model. (nih.gov)
- The cloned afu locus of Actinobacillus pleuropneumoniae restored the ability of an Escherichia coli K-12 mutant (aroB) to grow on iron-limited media. (nih.gov)
- Biomarkers of infection were screened for their possible role as evaluators of antibiotic treatment in an aerosol infection model of porcine pneumonia caused by Actinobacillus pleuropneumoniae (Ap). (dtu.dk)
- The apxIVA gene from the serotype 1 A. pleuropneumoniae type strain Shope 4074T encodes a protein with a predicted molecular mass of 202 kDa which has typical features of RTX proteins including hydrophobic domains in the N-terminal half and 24 glycine-rich nonapeptides in the C-terminal half that bind Ca2+. (nih.gov)
- Clustering of 26,012 predicted protein-coding genes showed that the pan genome of A. pleuropneumoniae consists of 3,303 gene clusters, which contain 1,709 core genome genes, 822 distributed genes, and 772 strain-specific genes. (asm.org)
- To evaluate lung lesions at slaughter after three-dose vaccination with a subunit Actinobacillus pleuropneumoniae vaccine containing ApxI, ApxII, ApxIII, and an outer membrane protein. (aasv.org)
- In reverse transcription PCR differential display (RT-PCR DD) experiments, A. pleuropneumoniae CM5 exposed to BALF up-regulated, among other genes, a gene predicted to encode LamB, an outer-membrane transport protein of the maltose regulon. (biomedcentral.com)
- These findings confirm that IVI antigens used as vaccine candidates provide partial protection against Actinobacillus pleuropneumoniae infection in a mouse model, which could be used as potential vaccine candidates in piglets. (frontiersin.org)
- For this, organ samples (blood, liver, spleen, kidney, tarsal and carpal joints, meninges, pleural and pericardial fluids) from weaners ( n = 47) infected experimentally with A. pleuropneumoniae serovar 7 by aerosol infection (infection dose: 10.9 × 10 3 cfu/animal) were examined by culture during the first week after infection. (biomedcentral.com)
- In conclusion, multi-organ spreading of A. pleuropneumoniae serovar 7 strain AP 76 seems to occur more frequently during acute infection following effective lung colonization than previously thought. (biomedcentral.com)
- To investigate the possible spreading of A. pleuropneumoniae to other organs than lungs forty-seven weaners experimentally infected with A. pleuropneumoniae serovar 7 from another infection trial were chosen by simple randomization. (biomedcentral.com)
- Production of proinflammatory mediators in the lungs is an important feature of A. pleuropneumoniae infection. (biomedcentral.com)
- Based on the hypothesis that AasP might play an important role in A. pleuropneumoniae adhesion and virulence by processing other surface-associated proteins, the predicted catalytic site of AasP was deleted and the isogenic mutant, AP76DeltaaasP, was compared to the wild-type strain in a biofilm assay as well as an aerosol infection model. (uni-bielefeld.de)
- Actinobacillus pleuropneumoniae (App) est l'agent étiologique de la pleuropneumonie porcine, une infection pulmonaire contagieuse chez les porcs. (umontreal.ca)
- Characterization of apxIVA, a new RTX determinant of Actinobacillus pleuropneumoniae. (nih.gov)
- Amplified fragment length polymorphism (AFLP) was evaluated as a method for genotypic characterization and subtyping within the bacterial species Actinobacillus pleuropneumoniae . (asm.org)
- QUEZADA, M y RUIZ, A . Serological behaviour study of Actinobacillus pleuropneumoniae (App) in commercial swine herds from the central region of Chile . (scielo.cl)
- Serological testing is widely used to monitor swine herds for Actinobacillus pleuropneumoniae (APP). (aasv.org)
- Broes A, Martineau GP, Gottschalk M. Dealing with unexpected Actinobacillus pleuropneumoniae serological results. (aasv.org)
- AFLP analysis provided a clear separation of A. lignieresii and A. pleuropneumoniae and divided the isolates of A. pleuropneumoniae into 20 clusters. (asm.org)
- A. pleuropneumoniae is genetically closely affiliated with Actinobacillus lignieresii ( 6 , 25 ), a bacterium commonly found in the mucosal membranes of cattle and the cause of chronic infections in the tongue and jaw (tree tongue). (asm.org)
- For this, we investigated possible spreading of A. pleuropneumoniae to body tissues other than the lungs in experimentally infected weaners. (biomedcentral.com)
- Actinobacillus pleuropneumoniae serovar 5b str. (uniprot.org)
- bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase [Actinobacillus pleuropneumoniae serovar 5b str. (cam.ac.uk)
- The Gram-negative bacterium Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumoniae, a lethal respiratory infectious disease causing great economic losses in the swine industry worldwide. (asm.org)
- A. pleuropneumoniae is a primary bacterial etiologic agent of porcine contagious pleuropneumonia, a severe respiratory disease leading to great economic losses to the global swine industry ( 7 ). (asm.org)
- Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, a significant disease that causes serious economic losses to the swine industry worldwide. (frontiersin.org)
- Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, a cause of considerable world wide economic losses in the swine industry. (duhnnae.com)
- Evaluar las lesiones pulmonares en el rastro después de una vacunación con tres dosis de una vacuna de Actinobacillus pleuropneumoniae de subunidades conteniendo ApxI, ApxII, ApxIII, y una proteína de membrana externa. (aasv.org)
- The goals of this study were to determine the role of A. pleuropneumoniae exotoxin ApxI in cytokine induction and to delineate the underlying mechanisms. (biomedcentral.com)
- Among various vaccines against Actinobacillus pleuropneumoniae, subunit vaccines using recombinant proteins of ApxI, ApxII, and ApxIII as vaccine antigens have shown good efficacy in terms of safety and protection. (bvsalud.org)
- Pleuropneumonia is caused by Actinobacillus pleuropneumoniae , a Gram negative rod-shaped bacterium which produces fimbriae (filaments), a protective capsule and at least four toxins (ApxI, ApxII, which are haemolytic and destroy red blood cells, ApxIII which kills cells and Apx IV, produced in vivo and essential for pathogenicity). (pigprogress.net)
- Thus, genes expressed by A. pleuropneumoniae in porcine BALF may be important for survival and pathogenesis of the organism. (biomedcentral.com)
- We also show that PGA is a substrate for dispersin B, a biofilm-releasing glycosyl hydrolase produced by A. actinomycetemcomitans , and that an orthologous dispersin B enzyme is produced by A. pleuropneumoniae . (asm.org)
- Our findings confirm that PGA functions as a biofilm matrix polysaccharide in phylogenetically diverse bacterial species and suggest that PGA may play a role in intercellular adhesion and cellular detachment and dispersal in A. actinomycetemcomitans and A. pleuropneumoniae biofilms. (asm.org)
- Although the precise role of this new RTX determinant in pathogenesis of porcine pleuropneumonia needs to be determined, apxIVA is the first in vivo induced toxin gene that has been described in A. pleuropneumoniae. (nih.gov)
- Global effects of catecholamines on Actinobacillus pleuropneumoniae gene expression. (uni-bielefeld.de)
- Since bronchoalveolar fluid (BALF) contains many of the innate immune and other components found in the lungs, we examined the gene expression of a virulent serovar 1 strain of A. pleuropneumoniae after exposure to concentrated BALF for 30 min. (biomedcentral.com)
- A. pleuropneumoniae was found to be the causative agent for up to 20% of all bacterial pneumonia cases in swine. (wikipedia.org)
- At slaughter, lungs were examined for gross and microscopic lesions and cultured for A pleuropneumoniae . (aasv.org)
- Differences in ADG and occurrence of A pleuropneumoniae -related lesions were compared between treatment groups using multilevel mixed models with room and pen as random effects. (aasv.org)
- Actinobacillus pleuropneumoniae -related lesions and ADG did not differ between control and treatment groups. (aasv.org)
- A robust antibody titer is essential in preventing lung lesions associated with Actinobacillus pleuropneumoniae (App). (izon.com)
- We sequenced the complete genome of A. pleuropneumoniae, JL03, an isolate of serotype 3 prevalent in China. (duhnnae.com)
- Attempts to integrate the pharmacokinetics (PK) of tiamulin concentrations in plasma with the pharmacodynamics (PD) measured as Minimum Inhibitory Concentration (MIC) against Actinobacillus pleuropneumoniae (App), using standardised MIC determinations, have been unsuccessful and led to considerations that lung 1 or leucocyte concentrations 2 might play a significant role, as estimated intracellular concentrations were 8.5µg/g but only 0.47µg/ml in plasma (ratio 18:1). (thepigsite.com)
- AasP, an autotransporter serine protease of Actinobacillus pleuropneumoniae, has been shown to be expressed in necrotic porcine lung tissue. (uni-bielefeld.de)
- To date, several factors involved in the virulence of A. pleuropneumoniae have been described, including Apx exotoxins, capsular polysaccharides (CPS), lipopolysaccharides (LPS), outer membrane proteins, iron-acquisition proteins and adhesin factors ( 11 , 19 , 24 ). (asm.org)
- A previous study indicated that lipopolysaccharides (LPS) extracted from Actinobacillus pleuropneumoniae bind two low-molecular-mass proteins, of approximately 10 and 11 kDa, present in porcine respiratory tract secretions (M. Bélanger, D. Dubreuil, and M. Jacques, Infect. (asm.org)
- Besides, biofilm formation was inhibited and drug sensitivity was increased by the addition of phenylalanine-arginine beta-naphthylamide (PAβN), a well-known efflux pump inhibitor (EPI), suggesting a role for EPI in antibacterial strategies toward drug tolerance of A. pleuropneumoniae . (frontiersin.org)
- In A. pleuropneumoniae , biofilm formation is prevalent among field isolates ( Kaplan and Mulks, 2005 ). (frontiersin.org)
- An isogenic Actinobacillus pleuropneumoniae AasP mutant exhibits altered biofilm formation but retains virulence. (uni-bielefeld.de)
- Tegetmeyer H, Fricke K, Baltes N. An isogenic Actinobacillus pleuropneumoniae AasP mutant exhibits altered biofilm formation but retains virulence. (uni-bielefeld.de)
- Absence of TolC Impairs Biofilm Formation in Actinobacillus pleuropneumoniae by Reducing Initial Attachment. (uni-bielefeld.de)
- Analysis on Actinobacillus pleuropneumoniae LuxS regulated genes reveals pleiotropic roles of LuxS/AI-2 on biofilm formation, adhesion ability and iron metabolism. (uni-bielefeld.de)
- Several virulence factors account for the remarkable pathogenicity of A. pleuropneumoniae. (wikipedia.org)
- Moreover, if MalT is directly or indirectly linked to the stringent response, an important global mechanism of bacterial persistence and virulence in many bacterial pathogens, it might play a role in A. pleuropneumoniae pathogenesis. (biomedcentral.com)
- Efficacy of a subunit vaccine against Actinobacillus pleuropneumoniae in an endemically infected swine herd. (aasv.org)
- A total of 430 newborn piglets in a herd endemically infected with A pleuropneumoniae were assigned to control and treatment groups by block randomization at litter level. (aasv.org)
- The aim of the present study was to detect quantitative trait loci (QTL) associated with resistance/susceptibility to A. pleuropneumoniae. (deepdyve.com)
- The results indicate the genetic background of A. pleuropneumoniae resistance in swine and provide new insights into the genetic architecture of resistance/susceptibility to porcine pleuropneumonia. (deepdyve.com)
- Actinobacillus pleuropneumoniae genes expression in biofilms cultured under static conditions and in a drip-flow apparatus. (uni-bielefeld.de)
- To determine the role of the lamB and other genes of the maltose regulon in the pathogenesis of A. pleuropneumoniae , knockout mutations were created in the lamB and malT genes, the latter being the positive transcriptional regulator of the maltose regulon. (biomedcentral.com)
- To identify A. pleuropneumoniae genes that are expressed in a medium that mimics, at least in part, the alveolar surface environment of the lungs, we incubated the bacterium in concentrated porcine bronchoalveolar lavage fluid (BALF). (biomedcentral.com)
- In RT-PCR DD experiments, A. pleuropneumoniae CM5 exposed to BALF for 30 min differentially expressed a number of genes, including seemingly a lamB homolog. (biomedcentral.com)
- The floR genes were detected in 8 (100%) of the 8 florfenicol-resistant A. pleuropneumoniae isolates. (bvsalud.org)
- Isolation of A. pleuropneumoniae from nasal cavities or tonsils from carrier animals is complicated due to the mixed bacterial flora present. (dtu.dk)
- To investigate the associations of serovar diversity with the underlying genetic components, more serovar-related genomic islands involved in the biosynthesis of capsular and lipopolysaccharide antigens should be decoded at the pan-genome level of A. pleuropneumoniae . (asm.org)
- Antigenic differences in the LPS can further determine A. pleuropneumoniae subtypes within a same capsular serovar ( 13 ). (asm.org)
- Immunoelectron microscopy showed binding of pig hemoglobin at the surface of all A. pleuropneumoniae isolates as well as labeling of outer membrane blebs. (asm.org)
- Improved diagnostic PCR assay for Actinobacillus pleuropneumoniae based on the nucleotide sequence of an outer membrane lipoprotein. (semanticscholar.org)
- O segundo segmento diferencial foi identificado apenas no genoma de A. pleuropneumoniae sorotipo 7 (AP76) e contém sequências como transposases caracterizando a presença de uma sequência de inserção no segmento. (ufv.br)
- Schematic comparison of florfenicol resistance plasmid pM3446F from A. pleuropneumoniae with plasmids pAQU1 (from Photobacterium damselae , subsp. (cdc.gov)
- Swine pleuropneumonia caused by Actinobacillus pleuropneumoniae (APP) is a highly contagious pulmonary disease that may cause important economic losses. (idexx.com)
- Under controlled conditions, 170 F2 animals of a Hampshire/Landrace family, with known differences in founder populations regarding A. pleuropneumoniae resistance, were challenged with an A. pleuropneumoniae serotype 7 aerosol followed by a detailed clinical, radiographic, ultrasonographic, pathological and bacteriological examination. (deepdyve.com)
- Waldmann, Karl 2014-01-21 00:00:00 Actinobacillus pleuropneumoniae is among the most important pathogens worldwide in pig production. (deepdyve.com)