A species of gram-negative, facultatively anaerobic coccobacillus-shaped bacteria that has been isolated from pneumonic lesions and blood. It produces pneumonia with accompanying fibrinous pleuritis in swine.
Infections with bacteria of the genus ACTINOBACILLUS.
A genus of PASTEURELLACEAE described as gram-negative, nonsporeforming, nonmotile, facultative anaerobes. Most members are found both as pathogens and commensal organisms in the respiratory, alimentary, and genital tracts of animals.
Inflammation of the lung parenchyma that is associated with PLEURISY, inflammation of the PLEURA.
Diseases of domestic swine and of the wild boar of the genus Sus.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
A species of Gram-negative, facultatively anaerobic spherical or rod-shaped bacteria indigenous to dental surfaces. It is associated with PERIODONTITIS; BACTERIAL ENDOCARDITIS; and ACTINOMYCOSIS.
A genus of PASTEURELLACEAE that consists of several species occurring in animals and humans. Its organisms are described as gram-negative, facultatively anaerobic, coccobacillus or rod-shaped, and nonmotile.
A species of gram-negative bacteria in the genus ACTINOBACILLUS. It is mainly a pathogen of PIGS, but also can infect HORSES.
Proteins from BACTERIA and FUNGI that are soluble enough to be secreted to target ERYTHROCYTES and insert into the membrane to form beta-barrel pores. Biosynthesis may be regulated by HEMOLYSIN FACTORS.
Process of determining and distinguishing species of bacteria or viruses based on antigens they share.
A class of carrier proteins that bind to TRANSFERRIN. Many strains of pathogenic bacteria utilize transferrin-binding proteins to acquire their supply of iron from serum.
Substances that are toxic to cells; they may be involved in immunity or may be contained in venoms. These are distinguished from CYTOSTATIC AGENTS in degree of effect. Some of them are used as CYTOTOXIC ANTIBIOTICS. The mechanism of action of many of these are as ALKYLATING AGENTS or MITOSIS MODULATORS.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria normally found in the flora of the mouth and respiratory tract of animals and birds. It causes shipping fever (see PASTEURELLOSIS, PNEUMONIC); HEMORRHAGIC BACTEREMIA; and intestinal disease in animals. In humans, disease usually arises from a wound infection following a bite or scratch from domesticated animals.
A pleuropneumonia of cattle and goats caused by species of MYCOPLASMA.
An enzyme that catalyzes the conversion of aspartic acid to ammonia and fumaric acid in plants and some microorganisms. EC 4.3.1.1.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
A family of coccoid to rod-shaped nonsporeforming, gram-negative, nonmotile, facultatively anaerobic bacteria that includes the genera ACTINOBACILLUS; HAEMOPHILUS; MANNHEIMIA; and PASTEURELLA.
Proteins found in any species of bacterium.
A subtype of bacterial transferrin-binding protein found in bacteria. It forms a cell surface receptor complex with TRANSFERRIN-BINDING PROTEIN A.
Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.
The oldest recognized genus of the family PASTEURELLACEAE. It consists of several species. Its organisms occur most frequently as coccobacillus or rod-shaped and are gram-negative, nonmotile, facultative anaerobes. Species of this genus are found in both animals and humans.
A round-to-oval mass of lymphoid tissue embedded in the lateral wall of the PHARYNX. There is one on each side of the oropharynx in the fauces between the anterior and posterior pillars of the SOFT PALATE.
Macrolide antibiotic obtained from cultures of Streptomyces fradiae. The drug is effective against many microorganisms in animals but not in humans.
A genus of gram-negative bacteria in the genus ACTINOBACILLUS, which is pathogenic for HORSES and PIGS.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The functional hereditary units of BACTERIA.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
An envelope of loose gel surrounding a bacterial cell which is associated with the virulence of pathogenic bacteria. Some capsules have a well-defined border, whereas others form a slime layer that trails off into the medium. Most capsules consist of relatively simple polysaccharides but there are some bacteria whose capsules are made of polypeptides.
Proteins that specifically bind to IRON.
Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
Proteins isolated from the outer membrane of Gram-negative bacteria.
A FERREDOXIN-dependent oxidoreductase that is primarily found in PLANTS where it plays an important role in the assimilation of SULFUR atoms for the production of CYSTEINE and METHIONINE.
Polysaccharides found in bacteria and in capsules thereof.
A species of gram-negative bacteria in the genus HAEMOPHILUS, causing respiratory tract disease in CHICKENS known as infectious coryza.
Infections with bacteria of the genus PASTEURELLA.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria normally commensal in the flora of CATTLE and SHEEP. But under conditions of physical or PHYSIOLOGICAL STRESS, it can cause MASTITIS in sheep and SHIPPING FEVER or ENZOOTIC CALF PNEUMONIA in cattle. Its former name was Pasteurella haemolytica.

Pulmonary lesions in guinea pigs experimentally infected with Actinobacillus pleuropneumoniae (A.p.) serovar 1. (1/288)

Pathological studies were carried out on the lungs of guinea pigs intratracheally inoculated with 4.6 x 10(6-8) colony forming units (CFU)/head of Actinobacillus pleuropneumoniae serovar 1. All animals in the highest dose group died within 24 hr post inoculation (hpi) and showed pulmonary lesions being hemorrhagic in nature while all animals in the lowest dose group were killed as scheduled at 11 days post inoculation (dpi) and showed only hyperplasia of peribronchial lymphoid tissues. In the middle dose group, two died within 24 hpi, two died at 9 dpi, and the remaining one was killed at 11 dpi. Two guinea pigs which died at 9 dpi showed fibrinonecrotic pleuropneumonia which is the most characteristic acute pulmonary lesion in swine, and has not yet been reproduced in laboratory animals up to the present time. This suggests that guinea pigs may be a useful laboratory animal for studying the pathogenesis of Actinobacillus pleuropneumoniae infection in swine.  (+info)

Vaccination and protection of pigs against pleuropneumonia with a vaccine strain of Actinobacillus pleuropneumoniae produced by site-specific mutagenesis of the ApxII operon. (2/288)

The production of toxin (Apx)-neutralizing antibodies during infection plays a major role in the induction of protective immunity to Actinobacillus pleuropneumoniae reinfection. In the present study, the gene encoding the ApxII-activating protein, apxIIC, was insertionally inactivated on the chromosome of a serovar 7 strain, HS93. Expression of the structural toxin, ApxIIA, and of the two genes required for its secretion, apxIB and apxID, still occurs in this strain. The resulting mutant strain, HS93C- Ampr, was found to secrete the unactivated toxin. Pigs vaccinated with live HS93C- Ampr via the intranasal route were protected against a cross-serovar challenge with a virulent serovar 1 strain of A. pleuropneumoniae. This is the first reported vaccine strain of A. pleuropneumoniae which can be delivered live to pigs and offers cross-serovar protection against porcine pleuropneumonia.  (+info)

Molecular cloning and sequencing of the aroA gene from Actinobacillus pleuropneumoniae and its use in a PCR assay for rapid identification. (3/288)

The gene (aroA) of Actinobacillus pleuropneumoniae, serotype 2, encoding 5-enolpyruvylshikimate-3-phosphate synthase was cloned by complementation of the aroA mutation in Escherichia coli K-12 strain AB2829, and the nucleotide sequence was determined. A pair of primers from the 5' and 3' termini were selected to be the basis for development of a specific PCR assay. A DNA fragment of 1,025 bp was amplified from lysed A. pleuropneumoniae serotypes 1 to 12 of biovar 1 or from isolated DNA. No PCR products were detected when chromosomal DNAs from other genera were used as target DNAs; however, a 1,025-bp DNA fragment was amplified when Actinobacillus equuli chromosomal DNA was used as a target, which could be easily differentiated by its NAD independence. The PCR assay developed was very sensitive, with lower detection limits of 12 CFU with A. pleuropneumoniae cells and 0.8 pg with extracted DNA. Specificity and sensitivity make this PCR assay a useful method for the rapid identification and diagnosis of A. pleuropneumoniae infections.  (+info)

Actinobacillus pleuropneumoniae osteomyelitis in pigs demonstrated by fluorescent in situ hybridization. (4/288)

Necrotizing osteomyelitis and fibrinopurulent arthritis with isolation of Actinobacillus pleuropneumoniae serotype 2 is reported in two pigs from a herd with lameness and mild coughing problems among 8 to 12-week-old pigs. Application of fluorescent in situ hybridization targeting 16S ribosomal RNA of A. pleuropneumoniae in formalin-fixed tissue was performed to verify the association of A. pleuropneumoniae with the bone and joint lesions. By in situ hybridization A. pleuropneumoniae was demonstrated as multiple microcolonies or single cells dispersed in focal fibrinonecrotizing pleuropneumonia, in joints with arthritis, and in bone necroses including lysis of growth plate and suppurative inflammation in the adjacent trabecular metaphysis, thus demonstrating that well-known infections manifest new, unusual lesions.  (+info)

A novel enzyme-linked immunosorbent assay using the recombinant Actinobacillus pleuropneumoniae ApxII antigen for diagnosis of pleuropneumonia in pig herds. (5/288)

For the surveillance of pig herds infected with porcine pleuropneumonia, an enzyme-linked immunosorbent assay (ELISA) using the recombinant Actinobacillus pleuropneumoniae ApxII protein as species- but not serotype-specific antigen was developed. Using this ELISA, 243 of 400 animals from 22 A. pleuropneumoniae-infected herds were classified as seropositive.  (+info)

First chromosomal restriction map of Actinobacillus pleuropneumoniae and localization of putative virulence-associated genes. (6/288)

Combined physical and genetic maps of the genomes of Actinobacillus pleuropneumoniae AP76 (serotype 7 clinical isolate) and of A. pleuropneumoniae ATCC 27088 (serotype 1 reference strain) were constructed by using the restriction endonucleases ApaI, AscI, NotI, and SalI. The chromosome sizes as determined by the addition of estimated fragment sizes were 2.4 Mbp, and both maps had a resolution of approximately 100 kbp. The linkages between the ApaI, AscI, NotI, and SalI fragments and their relative positions were determined by (i) fragment excision and redigestion and (ii) partial digests of defined fragments and Southern blot using end-standing probes. The single SalI site within the chromosome of strain A. pleuropneumoniae AP76 was defined as position 1 of the map; for the map of A. pleuropneumoniae ATCC 27088, the corresponding SalI site was chosen. Putative virulence-associated genes (apx, omlA, sodA, tbpBA, ureC, and a repeat element) and housekeeping genes (glyA, metJ, recA, and rhoAP) were positioned on the physical maps and located on the ApaI and NotI fragments of A. pleuropneumoniae serotype reference strains.  (+info)

Detection of antibodies against Actinobacillus pleuropneumoniae serotypes 1, 2, 5 and 7 using the immunohistochemical staining. (7/288)

Whole cells of Actinobacillus pleuropneumoniae (A. pleuropneumoniae) serotype 1, 2, 5 or 7 attached to fibrins were fixed in 10% neutral buffered formalin and embedded in paraffin. The sections on a slide glass were stained by the avidin-biotin complex immunoperoxidase (ABC) method. Test sera were applied to sections as primary antibodies. The serum antibodies against A.pleuropneumoniae (serotypes 1, 2, 5 and 7) were measured by the ABC method and complement fixation (CF) test. There was good correlation between the ABC and CF tests. The present results indicate that the immunohistochemical staining is as useful as the CF test for the detection and quantification of antibody in swine sera.  (+info)

Characterization of apxIVA, a new RTX determinant of Actinobacillus pleuropneumoniae. (8/288)

A fourth type of RTX determinant was identified in Actinobacillus pleuropneumoniae and was designated apxIVA. When expressed in Escherichia coli, recombinant ApxIVA showed a weak haemolytic activity and co-haemolytic synergy with the sphingomyelinase (beta-toxin) of Staphylococcus aureus. These activities required the presence of an additional gene, ORF1, that is located immediately upstream of apxIVA. The apxIVA gene product could not be detected in A. pleuropneumoniae cultures grown under various conditions in vitro; however, pigs experimentally infected with A. pleuropneumoniae serotypes 1, 5 and 7 started to produce antibodies that reacted with recombinant ApxIVA 14 d post-infection, indicating that apxIVA is expressed in vivo. In addition, sera from pigs naturally and experimentally infected with any of the serotypes all reacted with recombinant ApxIVA. The apxIVA gene from the serotype 1 A. pleuropneumoniae type strain Shope 4074T encodes a protein with a predicted molecular mass of 202 kDa which has typical features of RTX proteins including hydrophobic domains in the N-terminal half and 24 glycine-rich nonapeptides in the C-terminal half that bind Ca2+. The glycine-rich nonapeptides are arranged in a modular structure and there is some variability in the number of modules in the ApxIVA proteins of different serotypes of A. pleuropneumoniae. The deduced amino acid sequences of the ApxIVA proteins have significant similarity with the Neisseria meningitidis iron-regulated RTX proteins FrpA and FrpC, and to a much lesser extent with other RTX proteins. The apxIVA gene could be detected in all A. pleuropneumoniae serotypes and seems to be species-specific. Although the precise role of this new RTX determinant in pathogenesis of porcine pleuropneumonia needs to be determined, apxIVA is the first in vivo induced toxin gene that has been described in A. pleuropneumoniae.  (+info)

TY - JOUR. T1 - Actinobacillus pleuropneumoniae serotypes in Hungary. AU - Sárközi, Rita. AU - Makrai, L.. AU - Fodor, L.. PY - 2018/9/1. Y1 - 2018/9/1. N2 - A total of 255 Actinobacillus pleuropneumoniae isolates were collected from 634 lung samples representing 70 swine herds in Hungary between January 2012 and June 2016. On the basis of the indirect haemagglutination test 77 independent strains were included in the evaluation after the elimination of duplicate or multiple serotypes from the same herd. In the case of 7 herds strains of two different serotypes were identified. Fourteen Hungarian A. pleuropneumoniae isolates from the culture collection of the Department of Microbiology and Infectious Diseases, isolated before 2012, were also included in the evaluation (one each from 12 herds and two each from two herds, where two serotypes occurred). Out of the altogether 91 A. pleuropneumoniae strains 72 strains belonged to biotype I and 19 strains could be allocated to biotype II. In ...
In Denmark porcine pleuropneumonia is most frequently caused by Actinobacillus pleuropneumoniae serotype 2 (60%). Isolation of A. pleuropneumoniae from nasal cavities or tonsils from carrier animals is complicated due to the mixed bacterial flora present. An immunomagnetic separation technique (IMS) using immunomagnetic beads (Dynabeads(R)) was developed for isolation of A. pleuropneumoniae serotype 2 from pure cultures and from heterogeneous suspensions. Different coating and washing procedures were evaluated in pure and mixed cultures using polyclonal (PAb) and monoclonal antibodies. The highest reisolation yield was achieved when the beads were coated with 1.5 mug PAb IgG/10(7) beads. After washing the beads for four times 9-24% of the bacteria could be reisolated depending on the amount of IgG attached to the beads and the number of beads used. The recovery was increased to 19-61% when only two washing steps were performed. The IMS was further evaluated using dilutions of A. pleuropneumoniae ...
Actinobacillus pleuropneumoniae serotype 3 UPF0263 protein APJL_1366 (APJL_1366) datasheet and description hight quality product and Backed by our Guarantee
Actinobacillus pleuropneumoniae is a Gram-negative bacterium and a member of the Pasteurellaceae family. This bacterium is the causative agent of porcine pleuropneumonia, which is a highly contagious respiratory disease causing important economical losses to the worldwide pig industry. It has been shown that A. pleuropneumoniae can form biofilms on abiotic surfaces (plastic and glass). Although in vitro models are extremely useful to gain information on biofilm formation, these models may not be representative of the conditions found at the mucosal surface of the host, which is the natural niche of A. pleuropneumoniae. In this paper, we describe a method to grow A. pleuropneumoniae biofilms on the SJPL cell line, which represents a biotic surface. A non-hemolytic, non-cytotoxic mutant of A. pleuropneumoniae was used in our assays and this allowed the SJPL cell monolayers to be exposed to A. pleuropneumoniae for longer periods. This resulted in the formation of biofilms on the cell monolayer after
Serotypes 3 and 8 of Actinobacillus pleuropneumoniae, the aetiological agent of porcine pleuropneumonia, have been reported to predominate in the UK. Direct serotyping of isolates of the organism is typically determined by the immunological reactivity of rabbit serum to its surface polysaccharides, but the method has limitations, for example, cross-reactions between serotypes 3, 6 and 8. This study describes the development of a serotype 3-specific PCR, based on the capsule locus, which can be used in a multiplex format with the organisms specific gene apxIV. The PCR test was evaluated on 266 strains of A pleuropneumoniae and 121 strains of other organisms, including all the major respiratory bacterial pathogens of pigs. The test was highly specific and sensitive and should be useful for differentiating strains of serotypes 3, 6 and 8, and in seroprevalence and epidemiological surveys in regions where serotype 3 is prevalent, such as the UK.. ...
article{176028, author = {Haesebrouck, Freddy and Chiers, Koen and Van Overbeke, Ingrid and Ducatelle, Richard}, issn = {0378-1135}, journal = {VETERINARY MICROBIOLOGY}, language = {eng}, number = {2-4}, pages = {239--249}, title = {Actinobacillus pleuropneumoniae infections in pigs: the role of virulence factors in pathogenesis and protection.}, volume = {58}, year = {1997 ...
Actinobacillus pleuropneumoniae (previously Haemophilus pleuropneumoniae), is a Gram-negative, facultative anaerobic, respiratory pathogen found in pigs. It was first reported in 1957, and was formally declared to be the causative agent of porcine pleuropneumonia in 1964. It was reclassified in 1983 after DNA studies showed it was more closely related to A. lignieresii. A. pleuropneumoniae is a nonmotile, Gram-negative, encapsulated coccobacillus bacterium found in the Pasteurellaceae family. It exhibits β-hemolysis activity, thus explaining its growth on chocolate or blood agar, but must be supplemented with NAD (V factor) to facilitate growth for one of its biological variants (biovar 1). As a facultative anaerobic pathogen, A. pleuropneumoniae may need CO2 to grow. Depending on the biovar, the bacteria may or may not be positive for urease; both biovars are positive for porphyrin. A. pleuropneumoniae was found to be the causative agent for up to 20% of all bacterial pneumonia cases in ...
Fifty randomly selected fattening pig herds were studied to investigate the epidemiological characteristics of infections with Actinobacillus pleuropneumoniae serovars 2, 3 and 9, and to identify risk factors for their within-herd seroprevalences. Information about 13 farm characteristics was obtained by means of a questionnaire and used to assess potential risk factors for the percentage of slaughter pigs with antibodies against each of the three serovars. The presence of antibodies was measured with an indirect ELISA. The median within-herd seroprevalence for serovar 2 was 58 per cent (range 0 to 100 per cent), for serovar 3, 53 per cent (range 10 to 95 per cent), and for serovar 9, 35 per cent (range 5 to 100 per cent). All but one farm tested positive for A pleuropneumoniae serovar 2, and all the farms were positive for A pleuropneumoniae serovars 3 and 9. There was a positive association (P<0.05) between each pair of serovars. The within-herd seroprevalence of serovar 2 was ...
Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, a significant disease that causes serious economic losses to the swine industry worldwide. Persistent infections caused by bacterial biofilms are recalcitrant to treat because of the particular drug resistance of biofilm-dwelling cells. TolC, a key component of multidrug efflux pumps, are responsible for multidrug resistance in many Gram-negative bacteria. In this study, we identified two TolC-like proteins, TolC1 and TolC2, in A. pleuropneumoniae. Deletion of tolC1, but not tolC2, caused a significant reduction in biofilm formation, as well as increased drug sensitivity of both planktonic and biofilm cells. The genetic-complementation of the tolC1 mutation restored the competent biofilm and drug resistance. Besides, biofilm formation was inhibited and drug sensitivity was increased by the addition of phenylalanine-arginine beta-naphthylamide (PAβN), a well-known efflux pump inhibitor (EPI), suggesting a role
0004] It is commonly known how to produce the RTX-toxin ApxI by culturing Actinobacillus pleuropneumoniae in a culturing medium to which a calcium salt (i.e. a chemical compound, based on an acid, formed by replacing all or part of the hydrogen ions of the acid with one or more calcium ions) is added. In particular, EP 0 453 024 already describes such a method (see Example 2, paragraph 2 Purification and characterisation of hemolysin, subparagraph Methods). Note that ApxI used to be referred to as HLY (see Frey et al. in J Gen Microbiol. 1993 August; 139(8): 1723-8). From this EP patent it is known to add a calcium compound (CaCl2) to the medium. Indeed, in Microbiol Pathogenesis 37 (2004) 29-33 it is stated that transcriptional activity of the ApxI operon is enhanced by addition of calcium to the growth medium. This way, high levels of ApxI can be provided for. The medium should support the growth of APP bacteria. It is commonly known how to constitute a medium that supports growth of ...
Actinobacillus pleuropneumoniae ApaA protein: an antigenic membrane protein from Actinobacillus pleuropnemoniae homology to ABC transporters; amino acid sequence in first source
Pleuropneumonia infectioasa a porcului (PIP), cauzata de Actinobacillus pleuropneumoniae (App), poate fi intalnita la porcii de toate varstele, inclusiv la mistreti, dar simptomele clinice cele mai frecvente sunt inregistrate la porcii in faza de finisare, in varsta de peste 12 saptamani. In formele clasice de boala animalele manifesta febra si simptome respiratorii, datorate leziunilor caracteristice de pneumonie hemoragico-necrotica sau pleuropneumonie fibrinoasa. Boala poate evolua supra-acut, fara semne clinice, animalele fiind gasite moarte, acut, mai ales in efectivele naive imunologic, cu pierderi prin mortalitate de 15-20%, sau sub-acut, cu mortalitate mai redusa dar variabila. In efectivele infectate endemic boala se cronicizeaza la animalele care au supravietuit, afectand starea lor de bine si producand o scadere a performantelor zootehnice. Porcii pot fi purtatori fara sa dezvolte vreun raspuns imun. Boala genereaza pierderi economice insemnate.. ...
Biomarkers of infection were screened for their possible role as evaluators of antibiotic treatment in an aerosol infection model of porcine pneumonia caused by Actinobacillus pleuropneumoniae (Ap). Following infection of 12 pigs, clinical signs of pneumonia developed within 20 h, whereafter the animals received a single dose of either danofloxacin (2.5 mg/kg) or tiamulin (10 mg/kg). To test the discriminative properties of the biomarkers, the dosage regimens were designed with an expected difference in therapeutic efficacy in favour of danofloxacin. Accordingly, the danofloxacin-treated pigs recovered clinically within 24h after treatment, whereas tiamulin-treated animals remained clinically ill until the end of the study, 48 h after treatment. A similar Picture was seen for the biomarkers of infection. During the infection period, plasma C-reactive protein (CRP), interleukin-6 and haptoglobin increased, whereas plasma zinc, ascorbic acid and alpha-tocopherol decreased. In the ...
MUNOZ, D; QUEZADA, M y RUIZ, A. Serological behaviour study of Actinobacillus pleuropneumoniae (App) in commercial swine herds from the central region of Chile. Arch. med. vet. [online]. 2008, vol.40, n.2, pp.147-153. ISSN 0301-732X. http://dx.doi.org/10.4067/S0301-732X2008000200006.. In Chile, there was only one existing study on App. This study was designed to determine the maternal immunity duration, the age of seroconversion and the apparent and true prevalence in animals from 7 swine commercial herds. 60 samples were taken per herd and divided into 10 serum samples from animals of 4, 6,10,14,18and21 weeks of age, which were analyzed by ELISA®. Out of the 420 samples, 134 were positive with 112 of them belonging to pigs under 10 weeks of age while only 22 were from animals over 10 weeks of age, which seroconverted presumably due to a natural infection. Regarding maternal immunity duration it was found that around the 10th week of age the animals lose their colostrum antibodies. In ...
Actinobacillus pleuropneumoniae ATCC ® 33590™ Designation: Femo TypeStrain=True Application: Characterization Respiratory research
TY - JOUR. T1 - Enhancement of protective immune responses by oral vaccination with Saccharomyces cerevisiae expressing recombinant Actinobacillus pleuropneumoniae ApxIA or ApxIIA in mice. AU - Sung, Jae Shin. AU - Seung, Won Shin. AU - Mi, Lan Kang. AU - Deog, Yong Lee. AU - Yang, Moon Sik. AU - Jang, Yong Suk. AU - Han, Sang Yoo. PY - 2007/12. Y1 - 2007/12. N2 - We previously induced protective immune response by oral immunization with yeast expressing the ApxIIA antigen. The ApxI antigen is also an important factor in the protection against Actinobacillus pleuropneumoniae serotype 5 infection; therefore, the protective immunity in mice following oral immunization with Saccharomyces cerevisiae expressing either ApxIA (group C) or ApxIIA (group D) alone or both (group E) was compared with that in two control groups (group A and B). The immunogenicity of the rApxIA antigen derived from the yeast was confirmed by a high survival rate and an ApxIA-specific IgG antibody response (p , 0.01). The ...
Actinobacillus pleuropneumoniae, a Gram-negative, non-motile, facultatively anaerobic Pasteurellaceae, causes porcine pleuropneumonia, a highly contagious disease for which there is no effective vaccine. There are least twelve different serotypes, some of which produce no disease but others cause severe disease. Serotypes vary in different countries. Types 1, 5, 9, 11 and 12 are usually highly virulent and strains 3 and 6 are mild. The bacterium is carried in the tonsils and upper respiratory tract. It is transmitted short distances by droplet infection and only survives outside the pig for a few days.Actinobacillus pleuropneumoniae strain JL03 is an isolate of serotype 3 which is prevalent in China. Its genome is made up of a single circular chromosome encoding 2097 CDSs. It possesses a complete set of genes coding for enzymes of glycolysis, gluconeogenesis and non-oxidative pentose phosphate pathways. Genes coding for the key enzymes of the TCA cycle, citrate synthase, aconitase and isocitrate ...
ID ACTP7_1_PE100 STANDARD; PRT; 215 AA. AC ACTP7_1_PE100; B3GZU0; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Cytochrome c-type protein NrfB; (ACTP7_1.PE100). GN Name=nrfB; OrderedLocusNames=APP7_0100; OS ACTINOBACILLUS PLEUROPNEUMONIAE SEROVAR 7 STR. AP76. OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; OC Pasteurellaceae; Actinobacillus. OX NCBI_TaxID=537457; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS ACTP7_1.PE100. CC Actinobacillus pleuropneumoniae serovar 7 str. AP76, complete genome. CC sequence. CC -!- ANNOTATIONS ORIGIN:B3GZU0_ACTP7 CC -!- GENE_FAMILY: HOG000278525 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; B3GZU0; -. DR EMBL; CP001091; ACE60752.1; -; Genomic_DNA. DR RefSeq; YP_001967894.1; NC_010939.1. DR ProteinModelPortal; B3GZU0; -. DR STRING; B3GZU0; -. DR GeneID; 6397435; -. DR GenomeReviews; CP001091_GR; APP7_0100. DR ...
Shipping (test code: xtrnc) and handling (test code: xhand) fees are also applicable on each submission. External test price is subject to change. ...
Pharmacokinetic-pharmacodynamic (PK/PD) integration and modelling were used to predict dosage schedules for florfenicol for two pig pneumonia pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida. Pharmacokinetic data were pooled for two bioequivalent products, pioneer and generic formulations, administered intramuscularly to pigs at a dose rate of 15 mg/kg. Antibacterial potency was determined in vitro as minimum inhibitory concentration (MIC) and Mutant Prevention Concentration in broth and pig serum, for six isolates of each organism. For both organisms and for both serum and broth MICs, average concentration:MIC ratios over 48 h were similar and exceeded 2.5:1 and times greater than MIC exceeded 35 h. From in vitro time-kill curves, PK/PD modelling established serum breakpoint values for the index AUC24h/MIC for three levels of inhibition of growth, bacteriostasis and 3 and 4log10 reductions in bacterial count; means were 25.7, 40.2 and 47.0 h, respectively, for P. multocida and 24.6,
SWISS-MODEL Repository entry for B3GZ69 (KTHY_ACTP7), Thymidylate kinase. Actinobacillus pleuropneumoniae serotype 7 (strain AP76)
SWISS-MODEL Repository entry for B3H073 (YQGF_ACTP7), Putative pre-16S rRNA nuclease. Actinobacillus pleuropneumoniae serotype 7 (strain AP76)
Complete article is available online.. PDF version is available online.. Objective: To evaluate the duration of efficacy of a single dose of ceftiofur crystalline free acid sterile suspension (CCFA-SS) against clinical disease in grower pigs inoculated intratracheally with Actinobacillus pleuropneumoniae.. Materials and methods: One hundred and ninety male piglets were blocked by weight and randomly assigned to 19 pens of 10 animals each, with three pens randomly assigned to each of six treatments. Pigs were challenged with A pleuropneumoniae at 7 to 8 weeks old (Day 0). Groups 1 through 5 were treated with a single intramuscular dose of CCFA-SS (5 mg ceftiofur equivalents per kg body weight) on Days -13, -10, -7, -4, and -1, respectively. Group 6 pigs were challenged, untreated controls. A single pen of 10 unchallenged, untreated pigs acted as sentinels. The primary variable was removal rate (percent of pigs per pen that died or were euthanized because of severe illness by Day 9). Ancillary ...
Pourquier, P., Lesceu, S. Use of the ID Screen® APP indirect ELISA range for quick identification and serotyping of Actinobacillus pleuropneumoniae in domestic pigs. Poster presented at the 6th International Symposium on Emerging and Re-emerging Pig Diseases, June 2011, Madrid, Spain ...
Formulated to provide outstanding protection against the most common and virulent serotypes 1, 5 and 7. For prevention of pneumonia in pigs 4 weeks of age or older by infection with the prevalent serotypes of Actinobacillus pleuropneumoniae ...
With funding from Pork CRC, Australian scientists have developed a new one-shot vaccine delivery system against the endemic pig disease Actinobacillus pleuropneumoniae (APP).
Porcine pleuropneumonia, caused by Actinobacillus pleuropneumoniae, is a bacterial respiratory disease of swine. Acute outbreaks of the disease are often accompanied by high mortality and economic losses. As severe cases of the disease frequently require parenteral antibiotic treatment of the animals, the efficacy of a single, high dose of marbofloxacin was compared to a three-time application of a dose of enrofloxacin under experimental conditions. A blinded, controlled, randomized and blocked dose confirmation study was conducted to test the efficacy and safety of a single dose of 8 mg/kg marbofloxacin (160 mg/ml, Forcyl® Swine, Vetoquinol SA, France) to treat acute porcine pleuropneumonia after experimental aerosol inoculation of pigs with A. pleuropneumoniae serotype 2. The results were compared to a three consecutive day treatment of 2.5 mg/kg enrofloxacin and a mock (saline) treatment. Criteria for the assessment of efficacy were severity of lung lesions, bacteriological cure and the course of
LIU, Qiong et al. Construction and immunogenicity of a ΔapxIC/ompP2 mutant of Actinobacillus pleuropneumonias and Haemophilus parasuis. Onderstepoort j. vet. res. [online]. 2013, vol.80, n.1, pp.1-6. ISSN 2219-0635.. The apxIC genes of the Actinobacillus pleuropneumoniae serovar 5 (SC-1), encoding the ApxI-activating proteins, was deleted by a method involving sucrose counter-selection. In this study, a mutant strain of A. pleuropneumoniae (SC-1) was constructed and named AapxIC/ ompP2. The mutant strain contained foreign DNA in the deletion site of ompP2 gene of Haemophilus parasuis. It showed no haemolytic activity and lower virulence of cytotoxicity in mice compared with the parent strain, and its safety and immunogenicity were also evaluated in mice. The LD50 data shown that the mutant strain was attenuated 30-fold, compared with the parent strain (LD50 of the mutant strain and parent strain in mice were determined to be 1.0 x 107 CFU and 3.5 x 105 CFU respectively). The mutant strain that ...
The complete nucleotide sequence of a 7.7kb mobilisable plasmid (pM3446F), isolated from a florfenicol resistant isolate of Actinobacillus pleuropneumoniae, showed extended similarity to plasmids found in other members of the Pasteurellaceae containing the floR gene as well as replication and mobilisation genes. Mobilisation into other Pasteurellaceae species confirmed that this plasmid can be transferred horizontally ...
TY - JOUR. T1 - Optimal combinations of acute phase proteins for detecting infectious disease in pigs. AU - Heegaard, Peter M. H. AU - Stockmarr, Anders AU - Piñeiro, Matilde AU - Carpintero, Rakel AU - Lampreave, Fermin AU - Campbell, Fiona Margaret. AU - Eckersall, P. David AU - Toussaint, Mathilda J.M. AU - Gruys, Erik AU - Skall Sorensen, Nanna PY - 2011/3/17. Y1 - 2011/3/17. N2 - The acute phase protein (APP) response is an early systemic sign of disease, detected as substantial changes in APP serum concentrations and most disease states involving inflammatory reactions give rise to APP responses. To obtain a detailed picture of the general utility of porcine APPs to detect any disease with an inflammatory component seven porcine APPs were analysed in serum sampled at regular intervals in six different experimental challenge groups of pigs, including three bacterial (Actinobacillus pleuropneumoniae, Streptococcus suis, Mycoplasma hyosynoviae), one parasitic (Toxoplasma gondii) and one ...
Microbiology students of [http://www.biology.ucsd.edu/classes/bimm120.SP07/ Kit Pogliano and Rachel Larsen] at the University of California at San Diego are currently authoring the following pages as term projects for their course, BIMM120 Bacteriology.,br> ,table border=0 cellpadding=2 cellspacing=2 width=100%> ,tr valign=top> ,td> [[Acinetobacter baumannii]],br> By Jennifer Wang [[Actinobacillus pleuropneumoniae]],br> By Nhu Le [[Agrobacterium tumefaciens]],br> By Allan Wu [[Alcanivorax borkumensis]],br> By Justin Shih [[Anaeromyxobacter dehalogenans]],br> By Lori Nacario [[Anaplasma marginale]],br> By Patricia Shih [[Archaeoglobus fulgidus]],br> By Mike Sha [[Azoarcus sp. strain BH72]],br> By Caine Ballew [[Bacillus anthracis]], causes anthrax By Grace Ucar [[Bacillus clausii]],br> By Ankur Pate [[Bacillus licheniformis]],br> By Thu Mai [[Bacillus ...
Microbiology students of [http://www.biology.ucsd.edu/classes/bimm120.SP07/ Kit Pogliano and Rachel Larsen] at the University of California at San Diego are currently authoring the following pages as term projects for their course, BIMM120 Bacteriology.,br> ,table border=0 cellpadding=2 cellspacing=2 width=100%> ,tr valign=top> ,td> [[Acidothermus cellulolyticus]],br> By Chau Nguyen [[Acinetobacter baumannii]],br> By Jennifer Wang [[Actinobacillus pleuropneumoniae]],br> By Nhu Le [[Agrobacterium tumefaciens]],br> By Allan Wu [[Alcanivorax borkumensis]],br> By Justin Shih [[Anaeromyxobacter dehalogenans]],br> By Lori Nacario [[Anaplasma marginale]],br> By Patricia Shih [[Archaeoglobus fulgidus]],br> By Mike Sha [[Azoarcus sp. strain BH72]],br> By Caine Ballew [[Bacillus anthracis]], causes anthrax By Grace Ucar [[Bacillus clausii]],br> By Ankur Pate [[Bacillus ...
Welcome to the Inform a Colleague area of the Academy Website. The American Academy of Spine Physicians (AASP) strives to implement new and more efficient methods for members to communicate with other healthcare professionals. One of the most effective tools is the online Inform a Colleague function. This function can be used to expand and educate your spinecare referral network. It also represents a resource to conveniently inform others about trends in spinecare and about offers of organizations such as the American Academy of Spine Physicians (AASP), the International Spine Association (ISA). The link library also contains an application for membership with the AASP ...
Patrick G. Halbur and Eric Bush. Report: Results of the recent survey of the membership of the AASP for outbreaks of sow abortion and mortality ...
Yeah - I dont think its all one or the other - but what reason to not expect the worst administration in history (@ least our own)? Theres already damage to civil society - & his cabinet is full of Oligarchs & people determined to destroy the very departments theyre supposed to run. Hes surrounding himself w/ Generals & dispensing w/ Diplomacy...Were that it was only ridiculous. The country & the world are @ critical junctures - & the reins are being handed to a hate-filled racist nationalist business fraud, bankruptcy pest, & game show host famous for terminating people. But - this is not Unreality TV - this is the real stage ...
Structural studies of the O-chain of the phenol-phase soluble lipopolysaccharide from Haemophilus pleuropneumoniae serotype 2: Biochem.Cell Biol.
TY - JOUR. T1 - Effect of heat treatment on the surface antigens of Haemophilus pleuropneumoniae.. AU - Mittal, K. R.. AU - Higgins, Robert. AU - Larivière, S.. AU - Martineau, G. P.. PY - 1987/1/1. Y1 - 1987/1/1. N2 - Coagglutination and ring precipitation tests were used to study the effect of heat on the surface antigens of Haemophilus pleuropneumoniae strains employing the reference strains belonging to serotypes 1 to 7 and field isolates belonging to serotypes 1, 2, 3, 5, 6 and 7. By immunising rabbits with formalin-fixed whole-cell suspension, antibodies were obtained which sensitised Cowan I Staphylococcus aureus to coagglutinate antigen preparations which had not been heated, or heated at 56 degrees C, or boiled or autoclaved. Similar positive reactions were obtained with the ring precipitation test. Heating the cultures at 56 degrees C for one hour was best for exposing the most potent serotype-specific antigens in all the strains studied. All the reference strains and most of the ...
Looking for Actinobacillus capsulatus? Find out information about Actinobacillus capsulatus. A genus of gram-negative, immotile and nonspore-forming, oval to rod-shaped, often pleomorphic bacteria which occur as parasites or pathogens in mammals ,... Explanation of Actinobacillus capsulatus
TY - JOUR. T1 - Towards an understanding of equine pleuropneumonia. T2 - Factors relevant for control. AU - Racklyeft, D. J.. AU - Raidal, S.. AU - Love, D. N.. PY - 2000/5. Y1 - 2000/5. N2 - Objective: To review relevant literature on factors associated with the development of equine pleuropneumonia Design: A review of the literature using a range of databases including Current Contents, Medline, ChemAbstracts, Biological Abstracts and CAB and a comprehensive search strategy which involved use of keywords, author and subject category searches. Additional sources included review of articles cited by key accumulated references. Results: Since the early years of this century, many of the gaps in our knowledge of the pathogenesis of this disease have been filled. We now know that equine pleuropneumonia results from contamination of the lower respiratory tract with bacteria similar to the normal oropharyngeal microbiota of the horse and that transportation of any mode, especially over long ...
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Actinobacillus hominis.
Actinobacillus equulis: especie que se encuentra en la cavidad bucal y amígdalas del caballo y causa lesiones supurativas de los riñones y articulaciones de los cerdos y caballos y endocarditis en el cerdo ...
Learn about the veterinary topic of Pleuropneumonia in Horses. Find specific details on this topic and related topics from the Merck Vet Manual.
Mycoplasma hyopneumoniae is the causative agent of mycoplasmal pneumonia in swine. Infection with M. hyopneumoniae alone induces a mild, chronic pneumonia characterized clinically by mild cough. However, infection with M. hyopneumoniae alone is rare. M. hyopneumoniaes role in swine respiratory disease is generally through its interaction with other viruses and bacteria. M. hyopneumoniaes interaction with the other disease-causing organisms in the lungs of pigs results in either enzootic pneumonia or more recently, the porcine respiratory disease complex (PRDC). The most common organisms isolated from pigs infected with M. hyopneumoniae and exhibiting clinical signs of PRDC include porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus (SIV), Pasteurella multocida, Streptococcus species, Actinobacillus pleuropneumoniae, and Haemophilus parasuis, although any pathogen isolated from the lungs of pigs can be involved. While bacteria such as P. multocida appear to ...
Oral fluid analysis for herd monitoring is of interest to the commercial pig production in Korea. The aim of this study was to investigate pathogen-positive rates and correlations among eight pathogens associated with porcine respiratory disease complex by analyzing oral fluid samples from 214 pig groups from 56 commercial farms. Samples collected by a rope-chewing method underwent reverse-transcriptase polymerase chain reaction (RT-PCR) or standard polymerase chain reaction (PCR) analysis, depending on the microorganism. Pathogens were divided into virus and bacteria groups. The former consisted of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 (PCV2), and the latter Pasteurella multocida, Haemophilus parasuis, Actinobacillus pleuropneumoniae, Mycoplasma hyopneumoniae (MHP), Mycoplasma hyorhinis, and Streptococcus suis (SS). All pathogens were detected more than once by PCR. Age-based analysis showed the PCR-positive rate increased with increasing age for PCV2 ...
Periodontitis is mankinds most common chronic inflammatory disease. One severe form of periodontitis is localized aggressive periodontitis (LAP), a condition to which individuals of African origin demonstrate an increased susceptibility. The main causative organism of this disease is Actinobacillus …
Fingerprint Dive into the research topics of Isolation and characterization of the dnaKJ operon from Actinobacillus actinomycetemcomitans. Together they form a unique fingerprint. ...
Astăzi, conform manualului Bergey, în genul Actinobacillus, în afară de A. lignieresii şi A. suis, care produc bolile numite actinobaciloze, sunt alăturate şi alte 16 specii de bacterii (1). Acestea însă fie că produc entităţi diferite ca patogenie şi manifestări anatomoclinice, din care cauză poartă şi denumiri diferite, fie că produc nişte infecţii, în general rare, localizate şi manifestate altfel decât boala limbii de lemn produsă de A.lignieresii, fie că sunt prezente ca simpli germeni oportunişti, fără un rol patologic bine precizat. Dintre acestea, patologia veterinară este interesată de:. - A.pleuropneumoniae, care produce pleuropneumonia infecţioasă a porcului, ...
The use of the porcine lung to demonstrate recruitment maneuvers stimulated us to consider the possibility of using the ASL 5000 to investigate ways to improve protocols for the handling of ex-vivo perfused lungs. We believe this would provide interesting research projects for students and faculty. Many of the current ex-vivo perfusion protocols involve using positive pressure and high concentrations of oxygen to maintain isolated perfused lungs. The longest time that perfused lungs have been kept viable ex-vivo is about 10 hours. Using the ASL 5000 to model breathing, we would like to explore the possibility of extending survival time of ex-vivo perfused lungs. We believe that lowering the FiO2 and avoiding positive pressure ventilation could improve viability. Further development of this model could lead to advances in therapeutics related to airway clearance, mechanical ventilation, and airway pharmacology.. In the video below, the porcine lungs are attached to an Avea ventilator in the CPAP ...
The assumption that USS arise by mutation rather than copying was first confirmed using alignments of H. influenzae proteins with distant homologues. Abundant USS-like repeats were found in all eight Pasteurellacean genomes; the repeat consensuses of species in the Hin subclade were identical to that of H. influenzae (AAGTGCGGT), whereas members of the Apl subclade shared the consensus ACAAGCGGT. All species USSs had the strong consensus and flanking AT-rich repeats of H. influenzae USSs. DNA uptake and competition experiments demonstrated that the Apl-type repeat is a true USS distinct from the Hin-type USS: A. pleuropneumoniae preferentially takes up DNA fragments containing the Apl-type USS over both H. influenzae and unrelated DNAs, and H. influenzae prefers its own USS over the Apl type ...
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"Development of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae infection". Vaccine. 24 (49-50): 7226-7237. doi: ... Actinobacillus pleuropneumonia and Salmonella infections in pigs. Prior to the introduction of vaccination with material from ...
"Poly-N-acetylglucosamine mediates biofilm formation and antibiotic resistance in Actinobacillus pleuropneumoniae". Microb ... Kaplan JB, Ragunath C, Ramasubbu N, Fine DH (August 2003). "Detachment of Actinobacillus actinomycetemcomitans biofilm cells by ... Kaplan JB, Meyenhofer MF, Fine DH (2003). "Biofilm growth and detachment of Actinobacillus actinomycetemcomitans". J. Bacteriol ... a biofilm-releasing glycoside hydrolase from the periodontopathogen Actinobacillus actinomycetemcomitans". J. Mol. Biol. 349 (3 ...
At least the Actinobacillus pleuropneumoniae N-glycosyltransferase can also hydrolyze sugar-nucleotides in the absence of a ... N-glycosylation by Actinobacillus pleuropneumoniae HMW1C does not require metals, consistent with observations made on other ... The Actinobacillus pleuropneumoniae N-glycosyltransferase is the best researched enzyme of this family. Initially, protein ... N-glycosyltransferases from Actinobacillus pleuropneumoniae and Haemophilus influenzae use an asparagine-amino acid-serine or ...
Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae are pathogens that cause respiratory disease in swine. Haemophilus ... coli TOP10 strain Campylobacter jejuni Mycoplasma hyopneumoniae Haemophilus influenzae Actinobacillus pleuropneumoniae ...
... in Actinobacillus pleuropneumoniae". Front. Microbiol. 9: 2489. doi:10.3389/fmicb.2018.02489. PMC 6206304. PMID 30405558. ...
Clade II, encompassing Actinobacillus pleuropneumoniae, Actinobacillus minor, Haemophilus ducreyi, Mannheimia haemolytica and ... Two major clades are formed within this Pasteurellales: Clade I, encompassing Aggregatibacter, Pasteurella, Actinobacillus ...
Pigs: Treatment and metaphylaxis of swine respiratory disease (SRD) associated with Actinobacillus pleuropneumoniae, ...
Actinobacillus pleuropneumoniae, and Streptococcus suis. Mastitis-metritis-agalactia syndrome involved with E. coli, ...
... while the other includes Actinobacillus minor, Actinobacillus pleuropneumoniae, Haemophilus ducreyi, Haemophilus parasuis, and ... The distribution of CSIs corresponds to sensu stricto clades of "true" Actinobacillus, Haemophilus, and Pasteurella species, ... Other pathogenic members of the family Pasteurellaceae include Aggregatibacter, Mannheimia, Pasteurella, and Actinobacillus ... Actinobacillus, Haemophilus, and Pasteurella. These genera demonstrate extensive polyphyly across the family, however, CSIs ...
Actinobacillus equuli MeSH B03.440.450.009.580 - Actinobacillus pleuropneumoniae MeSH B03.440.450.009.675 - Actinobacillus ... Actinobacillus equuli MeSH B03.660.250.550.050.580 - Actinobacillus pleuropneumoniae MeSH B03.660.250.550.050.675 - ... Actinobacillus seminis MeSH B03.660.250.550.050.700 - Actinobacillus suis MeSH B03.660.250.550.290 - Haemophilus MeSH B03.660. ... Actinobacillus MeSH B03.660.250.550.050.050 - Actinobacillus actinomycetemcomitans MeSH B03.660.250.550.050.200 - ...
2008). "Actinobacillus pleuropneumoniae". Pasteurellaceae: Biology, Genomics and Molecular Aspects. Caister Academic Press. ... Actinobacillus (Pasteurella) ureae and A. hominis occur in the respiratory tracts of healthy humans and may be involved in the ... Actinobacillus is a genus of Gram-negative, nonmotile and non-spore-forming, oval to rod-shaped bacteria occurring as parasites ... ISBN 978-1-904455-24-0. Nørskov-Lauritsen N, Kilian M (September 2006). "Reclassification of Actinobacillus ...
... and Streptococcus suis or Actinobacillus pleuropneumoniae infection. Antibiotics such as ceftiofur, gentamicin, and ... Actinobacillus suis is a beta-haemolytic, Gram-negative bacterium of the family Pasteurellaceae. The bacterium has many strains ... Type strain of Actinobacillus suis at BacDive - the Bacterial Diversity Metadatabase (Articles with 'species' microformats, ... Actinobacillus suis, reviewed and published by Wikivet at http://en.wikivet.net/Actinobacillus_suis accessed 07/10/2011. ...
... (previously Haemophilus pleuropneumoniae), is a Gram-negative, facultative anaerobic, ... Actinobacillus pleuropneumoniae was found to be the causative agent for up to 20% of all bacterial pneumonia cases in swine. ... Actinobacillus pleuropneumoniae is a nonmotile, Gram-negative, encapsulated coccobacillus bacterium found in the family ... "Actinobacillus pleuropneumoniae". Iowa State University College of Veterinary Medicine. Retrieved 3 September 2018. Shope, RE ( ...
Actinobacillus pleuropneumoniae serotype identified in Isolate by NAA with probe detection. Value Set Concept Status. Retired. ...
... and Actinobacillus pleuropneumoniae. Preventive amoxicillin treatment of the piglets was done routinely at weaning. Due to the ...
Actinobacillus pleuropneumoniae serotypes 1+9+11 Ab/Positive control in Serum by Immunoassay ... Actinobacillus pleuropneumoniae serotypes 1+9+11 Ab/Positive control in Serum by Immunoassay. ... Actinobacillus pleuropneumoniae serotypes 1+9+11 Ab/Positive control in Serum by Immunoassay. ...
Actinobacillus pleuropneumoniae serotype 5 Ab [Titer] in Serum by Complement fixation. Value Set Concept Status. Retired. ...
Actinobacillus pleuropneumoniae (organism). Code System Preferred Concept Name. Actinobacillus pleuropneumoniae (organism). ...
Actinobacillus pleuropneumoniae Ab [Presence] in Serum by Complement fixation. Value Set Concept Status. Retired. ...
Actinobacillus pleuropneumoniae serotype 2 Ab/Positive control in Serum by Immunoassay. Concept Status. Published. ...
Actinobacillus pleuropneumoniae Ab [Presence] in Serum by Complement fixation. Concept Status. Published. ...
Actinobacillus pleuropneumoniae serotypes 4+5+7 Ab/Positive control in Serum by Immunoassay ... Actinobacillus pleuropneumoniae serotypes 4+5+7 Ab/Positive control in Serum by Immunoassay. ... Actinobacillus pleuropneumoniae serotypes 4+5+7 Ab/Positive control in Serum by Immunoassay. ...
Actinobacillus pleuropneumoniae serotype 1 Ab [Presence] in Serum. Concept Status. Published. Concept Status Date. 09/27/2022. ...
Actinobacillus pleuropneumoniae serotypes 1+2+9+11 Ab/Positive control in Serum by Immunoassay. ... Actinobacillus pleuropneumoniae serotypes 1+2+9+11 Ab/Positive control in Serum by Immunoassay. ... Actinobacillus pleuropneumoniae serotypes 1+2+9+11 Ab/Positive control in Serum by Immunoassay. ...
Perfil serológico del virus de influenza porcinA, Micoplasma hyopneumoniae y Actinobacillus pleuropneumoniae en granjas del ...
Actinobacillus pleuropneumoniae serotypes 1+2+9+11 Ab/Positive control in Serum by Immunoassay ... Actinobacillus pleuropneumoniae serotypes 1+2+9+11 Ab/Positive control in Serum by Immunoassay. ... Actinobacillus pleuropneumoniae serotypes 1+2+9+11 Ab/Positive control in Serum by Immunoassay. ...
Actinobacillus pleuropneumoniae serotype 3 Ab [Presence] in Serum by Immunoassay. Concept Status. Published. ...
Actinobacillus pleuropneumoniae (previously Haemophilus pleuropneumoniae), is a Gram-negative, facultative anaerobic, ... Actinobacillus pleuropneumoniae was found to be the causative agent for up to 20% of all bacterial pneumonia cases in swine. ... Actinobacillus pleuropneumoniae is a nonmotile, Gram-negative, encapsulated coccobacillus bacterium found in the family ... "Actinobacillus pleuropneumoniae". Iowa State University College of Veterinary Medicine. Retrieved 3 September 2018. Shope, RE ( ...
Immunity to Actinobacillus (Haemophilus) pleuropneumoniae in piglets Author: MacKay, David Keith John ISNI: 0000 0004 2690 ...
Actinobacillus pleuropneumoniae 1 Ab:PrThr:Pt:Ser:Ord. Known as: Actinobacillus pleuropneumoniae 1 Antibody:Presence or ... Actinobacillus pleuropneumoniae vaccines: from bacterins to new insights into vaccination strategies. *Mahendrasingh Ramjeet, ... OBJECTIVES To clarify the serological identity of the prototype strain of a group of Actinobacillus pleuropneumoniae isolates… ... Serological characterisation of Actinobacillus pleuropneumoniae isolated from pigs in 1993 to 1996. ...
Actinobacillus pleuropneumoniae (App) est lagent étiologique de la pleuropneumonie porcine, une infection pulmonaire ... Sélection de mutations affectant la formation de biofilm chez Actinobacillus pleuropneumoniae. Thesis or Dissertation ... A. pleuropneumoniae (App) is the causative agent of porcine pleuropneumonia, a contagious pulmonary infection in swine. Among ...
Gram T, Ahrens P. Improved diagnostic PCR assay for Actinobacillus pleuropneumoniae based on the nucleotide sequence of an ... The gene (omlA) coding for an outer membrane protein of Actinobacillus pleuropneumoniae serotypes 1 and 5 has been described ... abstract = "The gene (omlA) coding for an outer membrane protein of Actinobacillus pleuropneumoniae serotypes 1 and 5 has been ... N2 - The gene (omlA) coding for an outer membrane protein of Actinobacillus pleuropneumoniae serotypes 1 and 5 has been ...
Actinobacillus pleuropneumoniae. 99. Peptostreptococcus sp.. 99. 82. -. +. 29. +. 28. 5,3.103. Streptococcus genomosp. C4. 99. ...
Pages that link to "Actinobacillus pleuropneumoniae". ← Actinobacillus pleuropneumoniae. Special:WhatLinksHere/Actinobacillus ...
Palabras clave : Actinobacillus pleuropneumoniae; hematology; pathology. · resumen en Español · texto en Español · Español ( ... An experimental inoculation of Actinobacillus pleuropneumoniae (App) was carried out with a field isolate and an ATCC serotype ... and pathological study of inoculated pigs with a field isolate and an ATCC serotype 5 of Actinobacillus pleuropneumoniae. Arch ...
... were compared for the assessment of the different pathological outcomes derived from an Actinobacillus pleuropneumoniae (App) ... of four lung scoring systems for the assessment of the pathological outcomes derived from Actinobacillus pleuropneumoniae ... of four lung scoring systems for the assessment of the pathological outcomes derived from Actinobacillus pleuropneumoniae ...
Porcine pleuropneumonia caused by Actinobacillus pleuropneumoniae (APP) is a swine respiratory disease with an important impact ... iTRAQ-based quantitative proteomic analysis of peripheral blood serum in piglets infected with Actinobacillus pleuropneumoniae ...
... necrotic pleuropneumonia caused by Actinobacillus pleuropneumoniae and atrophic rhinitis caused by toxigenic Pasteurella ...
Actinobacillus pleuropneumoniae. Adenovirus. Alcaligenes faecalis. Alphavirus. Anaplasma marginale. Aphtovirus. Bacillus ... Haemophilus pleuropneumoniae. Haemophilus somnus. Herpes virus. Histomonas meleagrides. Influenza spp. virus. Leptospira ...
... serum resistance and biofilm formation of Actinobacillus pleuropneumoniae journal, February 2016 * Xie, Fang; Li, Gang; Zhang, ...
Actinobacillus pleuropneumoniae; deletion mutation; apxIBD gene; pnp gene; virulence Figure. *. Fig. 1. PCR analysis of the ... Li T, Zhang Q, Wang R, Zhang S, Pei J, Li Y, Li L, Zhou R. The roles of flp1 and tadD in Actinobacillus pleuropneumoniae pilus ... Liu J, Chen X, Lin L, Tan C, Chen Y, Guo Y, Jin M, Guo A, Bei W, Chen H. Potential use an Actinobacillus pleuropneumoniae ... Xie F, Li G, Zhou L, Zhang Y, Cui N, Liu S, Wang C. Attenuated Actinobacillus pleuropneumoniae double-deletion mutant S-8∆clpP/ ...
Transposon Mutagenesis in Actinobacillus Pleuropneumoniae with a Tn10 Derivative. J. Bacteriol. 1993, 175, 5717-5722. [Google ...
... and Actinobacillus pleuropneumoniae (A. pleuropneumoniae) in Young Pigs. PlosOne, http://dx.doi.org/10.1371/journal.pone. ...
Antikörper gegen Haemophilus (Actinobacillus) pleuropneumoniae in Blutseren aus niedersächsischen Schweinezuchtbeständen / ...
Identification of QTL affecting resistance/susceptibility to acute Actinobacillus pleuropneumoniae infection in swine.. Mamm ...
Airborne transmission of Actinobacillus pleuropneumoniae and porcine reproductive and respiratory syndrome virus in nursery ...
Research Topic: Functional characterization of the autotransporter adhesins of Actinobacillus pleuropneumoniae. Supervisor: ...
... in pneumonia in swine came from experimental infections with Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae. The ...
Method to grow Actinobacillus pleuropneumoniae biofilm on a biotic surface. BMC Vet Res 2013;9:213. [PMID: 24139070 DOI: ...
Actinobacillus pleuropneumoniae Controlled drug No Target species * Pigs Distribution category POM-V ...
Identification of an Outer Membrane Protein from Actinobacillus Pleuropneumoniae Serotype 7. Articles related to serotype 7 are ... Identification of an Outer Membrane Protein from Actinobacillus Pleuropneumoniae Serotype 7. Actinobacillus pleuropneumoniae ...
Actinobacillus pleuropneumoniae serotype 10 derived ApxI induces apoptosis in porcine alveolar macrophages. Chien, M. S., Chan ...
These boars will be free from Actinobacillus pleuropneumoniae (App), Porcine Reproductive and Respiratory Syndrome (PRRS) and ...
IDENTIFICATION OF A HEMOLYSIN FROM ACTINOBACILLUS-PLEUROPNEUMONIAE AND CHARACTERIZATION OF ITS CHANNEL PROPERTIES IN PLANAR ... A proteinaceous hemolysin secreted by strain 4074 of serotype 1 of Actinobacillus pleuropneumoniae was purified by ...
... the component of acute respiratory disease in swine associated with bacterial pathogens such as Actinobacillus pleuropneumoniae ... pleuropneumoniae, S. suis, P. multocida, Pseudorabies virus, Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), and/ ...
  • Actinobacillus pleuropneumoniae (previously Haemophilus pleuropneumoniae), is a Gram-negative, facultative anaerobic, respiratory pathogen found in pigs. (wikipedia.org)
  • CVM uses the term SRD to refer to the component of acute respiratory disease in swine associated with bacterial pathogens such as Actinobacillus pleuropneumoniae, Bordetella bronchiseptica, Haemophilus parasuis, Pasteurella multocida , or Streptococcus suis . (fda.gov)
  • The drug is prohibited from certain extra-label uses in the United States and Canada, however it may be approved in other countries for the treatment and metaphylaxis of swine respiratory disease (SRD) associated with Actinobacillus pleuropneumoniae, Pasteurella multocida, Bordetella bronchiseptica and Haemophilus parasuis sensitive to tildipirosin. (ghp-news.com)
  • Tenotryl TM (enrofloxacin) injectable solution is indicated for the treatment of swine respiratory disease (SRD) associated with Actinobacillus pleuropneumoniae , Pasteurella multocida , Haemophilus parasuis , Streptococcus suis, Bordetella bronchiseptica and Mycoplasma hyopneumoniae . (virbac.com)
  • Treatment and prevention for the Porcine Respiratory Complex (Actinobacillus pleuropneumoniae, Pasteurella multocida, Micoplasma hyopneumoniae, Streptococcus suis, Haemophilus parasuis). (farmabase.com)
  • O óleo íntegro teve sua capacidade antimicrobiana testada, por teste de difusão em ágar, contra 27 isolados de Escherichia coli provenientes de leite mastítico, um isolado de campo de Actinobacilus pleuropneumoniae e de Haemophilus parasuis além de cepas de referência de Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae, Shigella flexneri e Citrobacter freundi. (ufv.br)
  • infectious bovine keratoconjunctivitis (IBK) in cattle, an eye disease caused by Moraxella bovis;swine respiratory disease (SRD) in pigs caused by Actinobacillus pleuropneumoniae, Pasteurella multocida, Mycoplasma hyopneumoniae, Haemophilus parasuis and Bordetella bronchiseptica. (farmvetsupplies.com)
  • Nuflor®-S (florfenicol) Injectable Solution is indicated for treatment of swine respiratory disease associated with Actinobacillus pleuropneumoniae, Pasteurella multocida, Salmonella Choleraesuis, Streptococcus suis, Bordetella bronchiseptica , and Glaesserella (Haemophilus) parasuis in swine except for nursing piglets and swine of reproductive age intended for breeding. (merck-animal-health-usa.com)
  • Treatment of swine respiratory disease associated with Actinobacillus pleuropneumoniae and Pasteurella multocida. (nampharma.com)
  • Sensitive bacteria are mainly Pasteurella multocida , Pasteurella hemolytica , Actinobacillus pleuropneumoniae , Salmonella , E.coli , Streptococcus , Staphylococcus , etc. (jxfineway.com)
  • It is commonly used for the treatment of the cattle acute respiratory system infection (e.g. bronchopneumonia caused by Pasteurella ), bovine mastitis, pig actinobacillus pleuropneumonia, etc in veterinary clinical. (jxfineway.com)
  • Enrofloxacin, the main ingredient of Neotril-50 injection, is a quinolone-synthetic antibacterials containing fluorine(F) and that shows strong bactericidal efficacy against most of bacterial diseases like respiratory and digestive diseases caused by Mycoplasma as well as both of Gram-positive and Gram-negative like Actinobacillus, Pasteurella, E-coli, Salmonella, etc. (samu.co.kr)
  • It is additionally indicated for treatment of acute outbreaks of respiratory disease in pigs caused by strains of Actinobacillus pleuropneumoniae and Pasteurella multocida susceptible to florfenicol. (powerhorsevet.com)
  • Swine: Treatment of acute outbreaks of respiratory disease caused by strains of Actinobacillus pleuropneumoniae and Pasteurella multocida. (farmvetsupplies.com)
  • A. pleuropneumoniae (App) is the causative agent of porcine pleuropneumonia, a contagious pulmonary infection in swine. (umontreal.ca)
  • Actinobacillus pleuropneumoniae (APP) causes a form of porcine pleuropneumonia that leads to significant economic losses in the swine industry worldwide. (koreamed.org)
  • Actinobacillus pleuropneumoniae causes porcine pleuropneumonia, a disease which occurs world-wide and affects pigs of all ages. (annexpublishers.com)
  • This invention discloses the DNA sequences coding for the Actinobacillus pleuropneumonia hemolysin(s). (tamu.edu)
  • Current situation of Actinobacillus pleuropneumonia in Spain according to lung lesions scored at slaughterhouse using the Ceva Lung Program (CLP). (eaphm.org)
  • In Spain vaccination against Actinobacillus pleuropneumonia (Ap) is still at a relative low level, approximately 5% of the pigs, and antimicrobials (AMs) are often used to prevent or control bacterial diseases, but over the last years, pig producers are. (eaphm.org)
  • Pleuropneumonia is caused by the highly contagious respiratory tract pathogen Actinobacillus pleuropneumoniae resulting in lung infection and pneumonia. (farmhealthonline.com)
  • The comprehensive genetic and phylogenetic analysis shows that H. parasuis is closely related to Actinobacillus pleuropneumoniae and provides a foundation for future experimental confirmation of the virulence and pathogen-host interactions in H. parasuis. (pacb.com)
  • First evidence for the role of early cytokines in pneumonia in swine came from experimental infections with Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae. (nih.gov)
  • The effect of two different combinations of vaccines against Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae in pigs' lung health and. (eaphm.org)
  • Nineteen different serotype variants (serovars) have been recognized for A. pleuropneumoniae, based on the different capsular polysaccharides exhibited. (wikipedia.org)
  • Their identity was verified by sequencing approximately 500 bp of the amplification product from 50 of the A. pleuropneumoniae isolates, which all showed the expected DNA sequence characteristic of the serotype, To test the specificity of the reaction, 23 other bacterial species related to A. pleuropneumoniae or isolated from pigs were assayed. (dtu.dk)
  • Comparative hematological and pathological study of inoculated pigs with a field isolate and an ATCC serotype 5 of Actinobacillus pleuropneumoniae. (scielo.cl)
  • An experimental inoculation of Actinobacillus pleuropneumoniae (App) was carried out with a field isolate and an ATCC serotype 5. (scielo.cl)
  • Actinobacillus pleuropneumoniae (App) est l'agent étiologique de la pleuropneumonie porcine, une infection pulmonaire contagieuse chez les porcs. (umontreal.ca)
  • These boars will be free from Actinobacillus pleuropneumoniae (App), Porcine Reproductive and Respiratory Syndrome (PRRS) and mycoplasma. (pigprogress.net)
  • PRDC is mixed viral and bacterial infection of two or more respiratory pathogens, such as M. hyopneumoniae, A. pleuropneumoniae, S. suis, P. multocida , Pseudorabies virus, Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), and/or Swine Influenza Virus (SIV). (fda.gov)
  • A. pleuropneumoniae is a highly contagious and severe respiratory disease. (mechpath.com)
  • On this October episode of PigX Podcast, Dr. Derald Holtkamp and Dr. Marcelo Almeida join us to talk through their research on the bacteria Actinobacillus Pleuropneumoniae (APP). (piglivability.org)
  • Differences in virulence potential, immunogenicity, and worldwide geographical distribution contribute to the diversity of the A. pleuropneumoniae serotypes. (wikipedia.org)
  • The gene (omlA) coding for an outer membrane protein of Actinobacillus pleuropneumoniae serotypes 1 and 5 has been described earlier and has formed the basis for development of a specific PCR assay, The corresponding regions of all 12 A. pleuropneumoniae reference strains of biovar 1 were sequenced, Alignment of the sequences revealed conserved terminal and variable middle regions, which divided the reference strains into four distinct groups. (dtu.dk)
  • Comparative genomics of 26 complete circular genomes of 18 different serotypes of Actinobacillus pleuropneumoniae. (unibe.ch)
  • Actinobacillus pleuropneumoniae serovar 7 str. (up.ac.za)
  • Identification of QTL affecting resistance/susceptibility to acute Actinobacillus pleuropneumoniae infection in swine. (mpg.de)
  • Figure 1: Lungs infected with A. pleuropneumoniae after 21 days after initial infection. (mechpath.com)
  • Biofilm formation is prevalent among field isolates of Actinobacillus pleuropneumoniae. (semanticscholar.org)
  • nov. and recognition of a novel genomospecies for equine strains of Actinobacillus lignieresii. (semanticscholar.org)
  • The objectives of this study were to determine the serovar of a collection of Actinobacillus pleuropneumoniae strains within the 3-6-8-15 cross-reacting group and to analyze their phenotypic and genetic properties. (gob.ar)
  • Primers were selected from the conserved 5' and 3' termini of the gene, A 950-bp amplicon was obtained from each of 102 tested field isolates of A. pleuropneumoniae obtained from lungs. (dtu.dk)
  • Mycoplasma hyopneumoniae (Mhyo) and Actinobacillus pleuropneumoniae (A.p) are very common in swine farms worldwide and cause severe respiratory diseases with great economic impact. (eaphm.org)
  • As a facultative anaerobic pathogen, A. pleuropneumoniae may need CO2 to grow. (wikipedia.org)
  • A. pleuropneumoniae has a number of virulence factors that make it a successful pathogen. (mechpath.com)
  • Actinobacillus pleuropneumoniae was found to be the causative agent for up to 20% of all bacterial pneumonia cases in swine. (wikipedia.org)
  • This bacterium exists as a nonmotile, gram-negative coccobacillus and is the causative agent of pleuropneumoniae in pigs. (mechpath.com)
  • A. pleuropneumoniae continues to be a problem in various parts of the world but outbreaks have decreased in North America. (mechpath.com)
  • While biosecurity remains the best option for managing Actinobacillus pleuropneumoniae (APP) outbreaks in swine finishing barns, the recent APP outbreaks in the Midwest has spotlighted biosecurity gaps in this phase of production, according to Swine Vet Center veterinarian Dr. Ethan Spronk. (swinevetcenter.com)
  • Serological characterisation of Actinobacillus pleuropneumoniae isolated from pigs in 1993 to 1996. (semanticscholar.org)
  • Actinobacillus pleuropneumoniae is a nonmotile, Gram-negative, encapsulated coccobacillus bacterium found in the family Pasteurellaceae. (wikipedia.org)
  • Actinobacillus pleuropneumoniae , formerly known as Haemophilis pleuropneumoniae , is a member of the Pasteurellaceae family. (mechpath.com)
  • Several virulence factors account for the remarkable pathogenicity of A. pleuropneumoniae. (wikipedia.org)
  • They play an essential role for the pathogenicity of A. pleuropneumoniae . (mechpath.com)
  • The typical presentation of A. pleuropneumoniae in pigs is the characteristic demarcated lesions in the middle, cranial, and caudal lobes of the lungs. (wikipedia.org)
  • A. pleuropneumoniae , migrates to the swine lower respiratory system where it comes into contact with leukocytes inside the lungs. (mechpath.com)
  • LPS is found on the surface of A. pleuropneumoniae 's surface and acts as a toxin that causes tissue damage. (mechpath.com)
  • Figure 2: Lung infected by A. pleuropneumoniae showing pathological symptoms (a). (mechpath.com)
  • Respecto a la reposición, aparte de ser una fuente muy importante de introducción del virus, especialmente cuando el origen externo tiene un estatus PRRS positivo o desconocido, las nulíparas negativas también desempeñan un rol importante en el mantenimiento de la infección en una granja positiva, ya que actúan como una un flujo constante de animales susceptibles. (prrscontrol.com)
  • Además, el virus del PRRS puede sobrevivir en el agua durante más de una semana. (prrscontrol.com)
  • En la carne de cerdo, el virus del PRRS puede detectarse durante al menos una semana a 4ºC y durante meses en condiciones de congelación (-20ºC). (prrscontrol.com)
  • En algunos casos, la transmisión del virus del PRRS por el aire no se ha observado ni siquiera en cerdos que comparten un mismo espacio aéreo (en la misma nave). (prrscontrol.com)
  • A. pleuropneumoniae mainly colonizes tonsils in pigs where it can remain without causing disease ( Gottschalk, 2015 ). (farmhealthonline.com)
  • Les LDVV et LDM sont les seuls laboratoires de référence universitaire du Québec qui travaillent à l'identification des virus animaux et zoonotiques à l'aide des techniques classiques de virologie (isolement viral et la visualisation des particules virales en utilisant la microscopie électronique à transmission) et d'avant-garde (qPCR et séquençage à haut débit), respectivement. (umontreal.ca)
  • The specificity and sensitivity of this PCR compared to those of culture suggest the use of this PCR for routine identification of A. pleuropneumoniae. (dtu.dk)
  • Dans notre laboratoire, la formation de biofilms par App a été évaluée en microplaques dans différents milieux de culture. (umontreal.ca)
  • Cette banque de 1200 mutants a été criblée à l'aide du modèle in vitro de formation de biofilms en microplaques et en tubes : 24 mutants démontrant une formation de biofilms modifiée par rapport à la souche mère App 4074NalR ont été sélectionnés et identifiés, nous permettant ainsi de localiser le site d'insertion du transposon. (umontreal.ca)