Actinobacillus Infections: Infections with bacteria of the genus ACTINOBACILLUS.Actinobacillus: A genus of PASTEURELLACEAE described as gram-negative, nonsporeforming, nonmotile, facultative anaerobes. Most members are found both as pathogens and commensal organisms in the respiratory, alimentary, and genital tracts of animals.Actinobacillus pleuropneumoniae: A species of gram-negative, facultatively anaerobic coccobacillus-shaped bacteria that has been isolated from pneumonic lesions and blood. It produces pneumonia with accompanying fibrinous pleuritis in swine.Aggregatibacter actinomycetemcomitans: A species of Gram-negative, facultatively anaerobic spherical or rod-shaped bacteria indigenous to dental surfaces. It is associated with PERIODONTITIS; BACTERIAL ENDOCARDITIS; and ACTINOMYCOSIS.Actinobacillus suis: A species of gram-negative bacteria in the genus ACTINOBACILLUS. It is mainly a pathogen of PIGS, but also can infect HORSES.Pleuropneumonia: Inflammation of the lung parenchyma that is associated with PLEURISY, inflammation of the PLEURA.Actinobacillus equuli: A genus of gram-negative bacteria in the genus ACTINOBACILLUS, which is pathogenic for HORSES and PIGS.Swine Diseases: Diseases of domestic swine and of the wild boar of the genus Sus.Haemophilus: A genus of PASTEURELLACEAE that consists of several species occurring in animals and humans. Its organisms are described as gram-negative, facultatively anaerobic, coccobacillus or rod-shaped, and nonmotile.Aggressive Periodontitis: Inflammation and loss of PERIODONTIUM that is characterized by rapid attachment loss and bone destruction in the presence of little local factors such as DENTAL PLAQUE and DENTAL CALCULUS. This highly destructive form of periodontitis often occurs in young people and was called early-onset periodontitis, but this disease also appears in old people.Periodontitis: Inflammation and loss of connective tissues supporting or surrounding the teeth. This may involve any part of the PERIODONTIUM. Periodontitis is currently classified by disease progression (CHRONIC PERIODONTITIS; AGGRESSIVE PERIODONTITIS) instead of age of onset. (From 1999 International Workshop for a Classification of Periodontal Diseases and Conditions, American Academy of Periodontology)Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Aggregatibacter: A genus of PASTEURELLACEAE. Members are nonmotile, Gram-negative, facultatively anaerobic rods or coccobacilli. Its members are X factor (HEMIN) independent and variably dependent on V factor (NAD).Exotoxins: Toxins produced, especially by bacterial or fungal cells, and released into the culture medium or environment.Pasteurellaceae: A family of coccoid to rod-shaped nonsporeforming, gram-negative, nonmotile, facultatively anaerobic bacteria that includes the genera ACTINOBACILLUS; HAEMOPHILUS; MANNHEIMIA; and PASTEURELLA.Pasteurella: The oldest recognized genus of the family PASTEURELLACEAE. It consists of several species. Its organisms occur most frequently as coccobacillus or rod-shaped and are gram-negative, nonmotile, facultative anaerobes. Species of this genus are found in both animals and humans.Cytotoxins: Substances that are toxic to cells; they may be involved in immunity or may be contained in venoms. These are distinguished from CYTOSTATIC AGENTS in degree of effect. Some of them are used as CYTOTOXIC ANTIBIOTICS. The mechanism of action of many of these are as ALKYLATING AGENTS or MITOSIS MODULATORS.Serotyping: Process of determining and distinguishing species of bacteria or viruses based on antigens they share.Actinobacillosis: A disease characterized by suppurative and granulomatous lesions in the respiratory tract, upper alimentary tract, skin, kidneys, joints, and other tissues. Actinobacillus lignieresii infects cattle and sheep while A. equuli infects horses and pigs.Hemolysin Proteins: Proteins from BACTERIA and FUNGI that are soluble enough to be secreted to target ERYTHROCYTES and insert into the membrane to form beta-barrel pores. Biosynthesis may be regulated by HEMOLYSIN FACTORS.Actinobacillus seminis: A species of gram-negative bacteria in the genus ACTINOBACILLUS, which causes EPIDIDYMITIS in SHEEP.Bacterial Toxins: Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.Periodontal Diseases: Pathological processes involving the PERIODONTIUM including the gum (GINGIVA), the alveolar bone (ALVEOLAR PROCESS), the DENTAL CEMENTUM, and the PERIODONTAL LIGAMENT.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Gingiva: Oral tissue surrounding and attached to TEETH.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Bacterial Proteins: Proteins found in any species of bacterium.Transferrin-Binding Proteins: A class of carrier proteins that bind to TRANSFERRIN. Many strains of pathogenic bacteria utilize transferrin-binding proteins to acquire their supply of iron from serum.Dental Plaque: A film that attaches to teeth, often causing DENTAL CARIES and GINGIVITIS. It is composed of MUCINS, secreted from salivary glands, and microorganisms.Genes, Bacterial: The functional hereditary units of BACTERIA.

Pulmonary lesions in guinea pigs experimentally infected with Actinobacillus pleuropneumoniae (A.p.) serovar 1. (1/272)

Pathological studies were carried out on the lungs of guinea pigs intratracheally inoculated with 4.6 x 10(6-8) colony forming units (CFU)/head of Actinobacillus pleuropneumoniae serovar 1. All animals in the highest dose group died within 24 hr post inoculation (hpi) and showed pulmonary lesions being hemorrhagic in nature while all animals in the lowest dose group were killed as scheduled at 11 days post inoculation (dpi) and showed only hyperplasia of peribronchial lymphoid tissues. In the middle dose group, two died within 24 hpi, two died at 9 dpi, and the remaining one was killed at 11 dpi. Two guinea pigs which died at 9 dpi showed fibrinonecrotic pleuropneumonia which is the most characteristic acute pulmonary lesion in swine, and has not yet been reproduced in laboratory animals up to the present time. This suggests that guinea pigs may be a useful laboratory animal for studying the pathogenesis of Actinobacillus pleuropneumoniae infection in swine.  (+info)

Vaccination and protection of pigs against pleuropneumonia with a vaccine strain of Actinobacillus pleuropneumoniae produced by site-specific mutagenesis of the ApxII operon. (2/272)

The production of toxin (Apx)-neutralizing antibodies during infection plays a major role in the induction of protective immunity to Actinobacillus pleuropneumoniae reinfection. In the present study, the gene encoding the ApxII-activating protein, apxIIC, was insertionally inactivated on the chromosome of a serovar 7 strain, HS93. Expression of the structural toxin, ApxIIA, and of the two genes required for its secretion, apxIB and apxID, still occurs in this strain. The resulting mutant strain, HS93C- Ampr, was found to secrete the unactivated toxin. Pigs vaccinated with live HS93C- Ampr via the intranasal route were protected against a cross-serovar challenge with a virulent serovar 1 strain of A. pleuropneumoniae. This is the first reported vaccine strain of A. pleuropneumoniae which can be delivered live to pigs and offers cross-serovar protection against porcine pleuropneumonia.  (+info)

Actinobacillus pleuropneumoniae osteomyelitis in pigs demonstrated by fluorescent in situ hybridization. (3/272)

Necrotizing osteomyelitis and fibrinopurulent arthritis with isolation of Actinobacillus pleuropneumoniae serotype 2 is reported in two pigs from a herd with lameness and mild coughing problems among 8 to 12-week-old pigs. Application of fluorescent in situ hybridization targeting 16S ribosomal RNA of A. pleuropneumoniae in formalin-fixed tissue was performed to verify the association of A. pleuropneumoniae with the bone and joint lesions. By in situ hybridization A. pleuropneumoniae was demonstrated as multiple microcolonies or single cells dispersed in focal fibrinonecrotizing pleuropneumonia, in joints with arthritis, and in bone necroses including lysis of growth plate and suppurative inflammation in the adjacent trabecular metaphysis, thus demonstrating that well-known infections manifest new, unusual lesions.  (+info)

Agents of the "suis-ide diseases" of swine: Actinobacillus suis, Haemophilus parasuis, and Streptococcus suis. (4/272)

In recent years, Actinobacillus suis, Haemophilus parasuis, and Streptococcus suis have emerged as important pathogens of swine, particularly in high health status herds. Their association with a wide range of serious clinical conditions and has given rise to the moniker "suis-ide diseases." These organisms are early colonizers and, for that reason, are difficult to control by management procedures such as segregated early weaning. Vaccination, serodiagnostic testing, and even serotyping are complicated by the presence of multiple serotypes, cross-reactive antigens, and the absence of clear markers for virulence. In this review, we discuss our current understanding of the pathogenesis, epidemiology, and management of the causative agents of the "suis-ide diseases" of swine.  (+info)

Phenotypic variation in Actinobacillus actinomycetemcomitans during laboratory growth: implications for virulence. (5/272)

This study examined alteration of specific virulence traits associated with phenotypic changes seen when a low-passage disease-associated and well maintained parent strain of Actinobacillus actinomycetemcomitans was compared to a laboratory-grown spontaneous variant/mutant. Clinical isolates of A. actinomycetemcomitans recovered from periodontitis patients typically grow as rough, adherent colonies on primary culture but undergo transformation to smooth, non-adherent colonies following repeated passage in vitro. The relationship of these phenotypic changes to the virulence of the organism or to the processes that underlie this transformation are not understood. A fresh clinical isolate, designated strain CU1000, was obtained from the first molar site of a patient with classical signs of localized juvenile periodontitis and used as the parent strain to study virulence-related phenotypes. Following several passages of CU1000 on selective agar, a spontaneous variant that demonstrated smooth, opaque, non-adherent colonies was isolated and designated strain CU1060. This study compared the properties of these two strains with respect to colony morphology, autoaggregation, surface appendages, adherence to saliva-coated hydroxyapatite (SHA), LPS chemotype and activity, induction of fibroblast proteinase activity and antigenic properties. CU1000 demonstrated rough, raised, star-positive colonies which upon electron microscopic examination revealed the presence of large, flexible, bundled fibrils. In addition, CU1000 showed adherence to SHA, several unique protein antigens and elevated endotoxin and fibroblast proteinase activity. CU1060, on the other hand, showed minimal adherence to SHA and fewer reactive proteins compared to the fresh clinical isolates. This strain formed smooth, opaque colonies on agar, showed minimal fibril formation and limited endotoxin and fibroblast-proteinase-inducing activity. These findings demonstrate that clinical isolates of A. actinomycetemcomitans undergo significant virulence-reducing phenotypic alterations during in vitro passage and support the need to study this organism in its clinical form.  (+info)

Detection of antibodies against Actinobacillus pleuropneumoniae serotypes 1, 2, 5 and 7 using the immunohistochemical staining. (6/272)

Whole cells of Actinobacillus pleuropneumoniae (A. pleuropneumoniae) serotype 1, 2, 5 or 7 attached to fibrins were fixed in 10% neutral buffered formalin and embedded in paraffin. The sections on a slide glass were stained by the avidin-biotin complex immunoperoxidase (ABC) method. Test sera were applied to sections as primary antibodies. The serum antibodies against A.pleuropneumoniae (serotypes 1, 2, 5 and 7) were measured by the ABC method and complement fixation (CF) test. There was good correlation between the ABC and CF tests. The present results indicate that the immunohistochemical staining is as useful as the CF test for the detection and quantification of antibody in swine sera.  (+info)

Characterization of apxIVA, a new RTX determinant of Actinobacillus pleuropneumoniae. (7/272)

A fourth type of RTX determinant was identified in Actinobacillus pleuropneumoniae and was designated apxIVA. When expressed in Escherichia coli, recombinant ApxIVA showed a weak haemolytic activity and co-haemolytic synergy with the sphingomyelinase (beta-toxin) of Staphylococcus aureus. These activities required the presence of an additional gene, ORF1, that is located immediately upstream of apxIVA. The apxIVA gene product could not be detected in A. pleuropneumoniae cultures grown under various conditions in vitro; however, pigs experimentally infected with A. pleuropneumoniae serotypes 1, 5 and 7 started to produce antibodies that reacted with recombinant ApxIVA 14 d post-infection, indicating that apxIVA is expressed in vivo. In addition, sera from pigs naturally and experimentally infected with any of the serotypes all reacted with recombinant ApxIVA. The apxIVA gene from the serotype 1 A. pleuropneumoniae type strain Shope 4074T encodes a protein with a predicted molecular mass of 202 kDa which has typical features of RTX proteins including hydrophobic domains in the N-terminal half and 24 glycine-rich nonapeptides in the C-terminal half that bind Ca2+. The glycine-rich nonapeptides are arranged in a modular structure and there is some variability in the number of modules in the ApxIVA proteins of different serotypes of A. pleuropneumoniae. The deduced amino acid sequences of the ApxIVA proteins have significant similarity with the Neisseria meningitidis iron-regulated RTX proteins FrpA and FrpC, and to a much lesser extent with other RTX proteins. The apxIVA gene could be detected in all A. pleuropneumoniae serotypes and seems to be species-specific. Although the precise role of this new RTX determinant in pathogenesis of porcine pleuropneumonia needs to be determined, apxIVA is the first in vivo induced toxin gene that has been described in A. pleuropneumoniae.  (+info)

Genomic relatedness among Actinobacillus pleuropneumoniae field strains of sterotypes 1 and 5 isolated from healthy and diseased pigs. (8/272)

Forty-four Actinobacillus pleuropneumoniae isolates recovered from both healthy and diseased pigs were characterized by random amplified polymorphic DNA analysis (RAPD), pulsed field gel electrophoresis (PFGE) and apx toxin gene typing. Nine RAPD types and 14 PFGE patterns were identified. No common RAPD or PFGE patterns were found between strains of serotype 1 and those of serotype 5. The RAPD analysis indicated that the 15 serotype 1 strains isolated from diseased pigs were assigned to 4 RAPD types, with 66% of strains characterized by the same RAPD type. By contrast, the 5 strains of serotype 1 isolated from healthy carriers were dispersed in 4 RAPD types. These data suggest that the diversity of strains isolated from healthy pigs could be higher than that of strains recovered from diseased pigs. In addition, all serotype 5 strains exhibited a unique RAPD type. Unlike RAPD, PFGE analysis allowed discrimination among isolates of serotype 1 and among those of serotype 5. All but 3 isolates showed the same apx genotype as their respective serotype reference strain. These data indicate that RAPD analysis is a valuable rapid tool for routine subtyping of strains of serotype 1. For strains of serotype 5, a combination of several typing methods, such as PFGE and apx gene typing, is needed to provide useful information on the molecular epidemiology of swine pleuropneumonia.  (+info)

*Actinobacillus pleuropneumoniae

Overcrowding in pigpens, co-infections of other respiratory pathogens, and unusual stress all contribute to the spread of the ... Gillaspy, A. "Actinobacillus pleuropneumoniae". Laboratory for Molecular Biology and Cytometry Research-University of Oklahoma ... In 1995, A. pleuropneumoniae infections cost the US economy about $30 million. Such losses usually result from medication and ... "Actinobacillus pleuropneumoniae". Iowa State University College of Veterinary Medicine. Retrieved 20 April 2013. [permanent ...

*Vaccine

Actinobacillus pleuropneumonia and Salmonella infections in pigs. Vaccine development has several trends: Until recently,[when ... "Development of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae infection". Vaccine. 24 (49): 7226-32. doi: ... Vaccines can be prophylactic (example: to prevent or ameliorate the effects of a future infection by a natural or "wild" ... Lack of response commonly results from clinical factors such as diabetes, steroid use, HIV infection or age.[citation needed] ...

*Actinobacillus suis

Affected piglets can develop septicaemia, multifocal infections, respiratory signs, and may die. Adult pigs may show signs ... Actinobacillus suis is a beta-haemolytic, Gram-negative bacterium of the Pasteurellaceae family. The bacterium has many strains ... Type strain of Actinobacillus suis at BacDive - the Bacterial Diversity Metadatabase. ... Signs and necropsy findings may mimic diseases such as: Erysipelas, Glasser's Disease, Streptococcus suis and Actinobacillus ...

*Actinobacillosis

The infection is most commonly caused by abrasions on different soft tissues through which the bacteria, Actinobacillus ... One of the most common forms seen by veterinarians is mouth actinobacillosis of cattle, due to Actinobacillus lignieresii. The ... Actinobacillosis is a zoonotic disease caused by Actinobacillus. It is more commonly associated with animals than with humans. ... actinomycosis Actinobacillus suis "Merck Veterinary Manual". "Dorlands Medical Dictionary:actinobacillosis". "Actinobacillosis ...

*List of MeSH codes (C01)

... actinobacillus infections MeSH C01.252.400.700.030.178 --- actinobacillosis MeSH C01.252.400.700.433 --- haemophilus infections ... bacteroides infections MeSH C01.252.400.126 --- bartonellaceae infections MeSH C01.252.400.126.100 --- bartonella infections ... acinetobacter infections MeSH C01.252.400.610 --- mycoplasmatales infections MeSH C01.252.400.610.610 --- mycoplasma infections ... bordetella infections MeSH C01.252.400.143.740 --- whooping cough MeSH C01.252.400.155 --- borrelia infections MeSH C01.252. ...

*Cefquinome

All infections were cured. Intervet developed cefquinome (Cobactan) to treat bovine respiratory disease, the most common ... Actinobacillus pleuropneumoniae, and Streptococcus suis. Mastitis-metritis-agalactia syndrome involved with E. coli, ... For pigs, it is used to treat bacterial infections of the lungs and respiratory tract caused by P. multocida, Haemophilus ... To prevent the claimed infections in piglets, attention should be paid to hygiene and ventilation, and overcrowding should be ...

*Pasteurella bettyae

Sneath, P. H. A.; Stevens, M. (1990). "Actinobacillus rossii sp. nov., Actinobacillus seminis sp. nov., nom. rev., Pasteurella ... Pasteurella bettyae is a bacterium associated with human Bartholin gland abscess and finger infections. ...

*Aggregatibacter actinomycetemcomitans

Less frequently, A. actinomycetemcomitans is associated with non-oral infections such as endocarditis. Its role in aggressive ... Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans) is a Gram-negative, facultative ... It has also been isolated from actinomycotic lesions (mixed infection with certain Actinomyces species, in particular A. ... It was reclassified as Actinobacillus actinomycetemcomitans by Topley & Wilson (1929) and as Haemophilus actinomycetemcomitans ...

*HACEK organisms

In addition to valvular infections in the heart, they can also produce other infections such as bacteremia, abscess, ... HACEK originally referred to Haemophilus parainfluenzae, Haemophilus aphrophilus, Actinobacillus actinomycetemcomitans, ... which is an inflammation of the heart due to bacterial infection. HACEK is an abbreviation of the initials of the genera of ... and share an enhanced capacity to produce endocardial infections, especially in young children. Collectively they account for 5 ...

*Pharynx

Polyps or mucus can obstruct the nasopharynx, as can congestion due to an upper respiratory infection. The auditory tube, which ... The HACEK organisms (Haemophilus, Actinobacillus actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens, Kingella ... and share an enhanced capacity to produce endocardial infections, especially in young children. Fusobacterium is a pathogen. ...

*Dam methylase

Infection and Immunity. 69 (12): 7610-7615. doi:10.1128/iai.69.12.7610-7615.2001. ISSN 0019-9567. PMID 11705940. Malone, Thomas ... "Inactivation of DNA adenine methyltransferase alters virulence factors in Actinobacillus actinomycetemcomitans". Oral ...

*Jørgen Slots

"Comparison of Two Selective Media for Actinobacillus actinomycetemcomitans." J Clin Microbiol 1986;24:636-638. Fryman A, ... that substantiated aggressive periodontitis as a bona fide infection, rather than merely a degenerative disorder. Dental ... Slots is responsible for discovering the role of Aggregatibacter actinomycetemcomitans (previously Actinobacillus ...

*Dispersin B

... may be useful for treating and preventing biofilm-associated infections caused by poly-N-acetylglucosamine- ... Kaplan JB, Ragunath C, Ramasubbu N, Fine DH (August 2003). "Detachment of Actinobacillus actinomycetemcomitans biofilm cells by ... These catheters were shown to be as effective at preventing bacterial infection as already commercially available chlorhexidine ... Kaplan JB, Meyenhofer MF, Fine DH (2003). "Biofilm growth and detachment of Actinobacillus actinomycetemcomitans". J. Bacteriol ...

*Oral microbiology

Actinobacillus actinomycetemcomitans is considered an oral pathogen due to its virulence factors, its association with ... In addition, GI fungal infection is reported even among those patients with normal immune status. Digestive system-related ... ISBN 978-1-904455-24-0. Hajishengallis G, Russell MW (2008). "Molecular Approaches to Vaccination against Oral Infections". ... 2008). "Molecular Windows into the Pathogenic Properties of Actinobacillus actinomycetemcomitans". Molecular Oral Microbiology ...

*Aggressive periodontitis

Fives-Taylor, P. M.; Meyer, D. H.; Mintz, K. P.; Brissette, C. (June 1999). "Virulence factors of Actinobacillus ... Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious ... Zambon, J. J.; Christersson, L. A.; Slots, J. (December 1983). "Actinobacillus actinomycetemcomitans in human periodontal ... High levels of Aggregatibacter (or Actinobacillus) actinomycetemcomitans and, in some populations, Porphyromonas gingivalis. ...

*Kingella kingae

Infection has been shown to have high prevalence in the autumn and winter months. K.kingae infections are quite rare in adults ... Actinobacillus, Cardiobacterium, Eikenella, and Kingella organisms: a retrospective multicenter evaluation". J. Clin. Microbiol ... Poor oral hygiene, pharyngitis, and mucosal ulceration are also predisposing factors for infection. The infection can occur in ... The infection rate is poorly documented, thus the illness tends to go underdiagnosed. K. kingae can be transmitted person to ...

*Debridement (dental)

With the consistent and complete removal of biofilm, the infection can be arrested and healthy periodontium can be achieved. ... These bacteria include Porphyromonas gingivalis, Prevotella intermedia, and Actinobacillus actinomycetemcomitans. Healthy ...

*Sulfatide

... viral infection, and bacterial infection. As a result, sulfatide is associated with, able to bind to, and/or is present in ... Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae are pathogens that cause respiratory disease in swine. Haemophilus ... However, sulfatide has little function in HIV-1 infection of CD4+ cells. The binding of gp120 to GalCer has the ability to ... Sulfatide has been shown to play a role or have some association with several diseases and infections. This includes diabetes ...

*Mare reproductive loss syndrome

The question then became how exposure to the caterpillars produced these non-specific bacterial infections of the affected ... Actinobacillus encephalitis. MRLS was observed in mares of all breeds and ages. Early and late fetal losses were observed ... Mares which aborted are treated with broad-spectrum antibiotics to avoid bacterial infections. The foals born from mares ... Reviewing the speed with which ETCs produced late-term abortions in 2002 experiments, the nonspecific bacterial infections in ...
TY - JOUR. T1 - Actinobacillus pleuropneumoniae serotypes in Hungary. AU - Sárközi, Rita. AU - Makrai, L.. AU - Fodor, L.. PY - 2018/9/1. Y1 - 2018/9/1. N2 - A total of 255 Actinobacillus pleuropneumoniae isolates were collected from 634 lung samples representing 70 swine herds in Hungary between January 2012 and June 2016. On the basis of the indirect haemagglutination test 77 independent strains were included in the evaluation after the elimination of duplicate or multiple serotypes from the same herd. In the case of 7 herds strains of two different serotypes were identified. Fourteen Hungarian A. pleuropneumoniae isolates from the culture collection of the Department of Microbiology and Infectious Diseases, isolated before 2012, were also included in the evaluation (one each from 12 herds and two each from two herds, where two serotypes occurred). Out of the altogether 91 A. pleuropneumoniae strains 72 strains belonged to biotype I and 19 strains could be allocated to biotype II. In ...
In Denmark porcine pleuropneumonia is most frequently caused by Actinobacillus pleuropneumoniae serotype 2 (60%). Isolation of A. pleuropneumoniae from nasal cavities or tonsils from carrier animals is complicated due to the mixed bacterial flora present. An immunomagnetic separation technique (IMS) using immunomagnetic beads (Dynabeads(R)) was developed for isolation of A. pleuropneumoniae serotype 2 from pure cultures and from heterogeneous suspensions. Different coating and washing procedures were evaluated in pure and mixed cultures using polyclonal (PAb) and monoclonal antibodies. The highest reisolation yield was achieved when the beads were coated with 1.5 mug PAb IgG/10(7) beads. After washing the beads for four times 9-24% of the bacteria could be reisolated depending on the amount of IgG attached to the beads and the number of beads used. The recovery was increased to 19-61% when only two washing steps were performed. The IMS was further evaluated using dilutions of A. pleuropneumoniae ...
article{176028, author = {Haesebrouck, Freddy and Chiers, Koen and Van Overbeke, Ingrid and Ducatelle, Richard}, issn = {0378-1135}, journal = {VETERINARY MICROBIOLOGY}, language = {eng}, number = {2-4}, pages = {239--249}, title = {Actinobacillus pleuropneumoniae infections in pigs: the role of virulence factors in pathogenesis and protection.}, volume = {58}, year = {1997 ...
Serotypes 3 and 8 of Actinobacillus pleuropneumoniae, the aetiological agent of porcine pleuropneumonia, have been reported to predominate in the UK. Direct serotyping of isolates of the organism is typically determined by the immunological reactivity of rabbit serum to its surface polysaccharides, but the method has limitations, for example, cross-reactions between serotypes 3, 6 and 8. This study describes the development of a serotype 3-specific PCR, based on the capsule locus, which can be used in a multiplex format with the organisms specific gene apxIV. The PCR test was evaluated on 266 strains of A pleuropneumoniae and 121 strains of other organisms, including all the major respiratory bacterial pathogens of pigs. The test was highly specific and sensitive and should be useful for differentiating strains of serotypes 3, 6 and 8, and in seroprevalence and epidemiological surveys in regions where serotype 3 is prevalent, such as the UK.. ...
The purpose of this study was to test the hypothesis that diabetes aggravates periodontal destruction induced by Aggregatibacter actinomycetemcomitans infection. Thirty-eight diabetic and 33 normal rats were inoculated with A. actinomycetemcomitans and euthanized at baseline and at 4, 5, and 6 weeks after inoculation. Bone loss and the infiltration of polymorphonuclear leukocytes (PMNs) in gingival epithelium were measured in hematoxylin-eosin-stained sections. The induction of tumor necrosis factor alpha (TNF-alpha) was evaluated by immunohistochemistry and of apoptotic cells by a TUNEL (terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling) assay. After A. actinomycetemcomitans infection, the bone loss in diabetic rats was 1.7-fold and the PMN infiltration 1.6-fold higher than in normoglycemic rats (P , 0.05). The induction of TNF-alpha was 1.5-fold higher and of apoptotic cells was up to 3-fold higher in diabetic versus normoglycemic rats (P , 0.05). Treatment with a ...
Actinobacillus pleuropneumoniae (previously Haemophilus pleuropneumoniae), is a Gram-negative, facultative anaerobic, respiratory pathogen found in pigs. It was first reported in 1957, and was formally declared to be the causative agent of porcine pleuropneumonia in 1964. It was reclassified in 1983 after DNA studies showed it was more closely related to A. lignieresii. A. pleuropneumoniae is a nonmotile, Gram-negative, encapsulated coccobacillus bacterium found in the Pasteurellaceae family. It exhibits β-hemolysis activity, thus explaining its growth on chocolate or blood agar, but must be supplemented with NAD (V factor) to facilitate growth for one of its biological variants (biovar 1). As a facultative anaerobic pathogen, A. pleuropneumoniae may need CO2 to grow. Depending on the biovar, the bacteria may or may not be positive for urease; both biovars are positive for porphyrin. A. pleuropneumoniae was found to be the causative agent for up to 20% of all bacterial pneumonia cases in ...
Fifty randomly selected fattening pig herds were studied to investigate the epidemiological characteristics of infections with Actinobacillus pleuropneumoniae serovars 2, 3 and 9, and to identify risk factors for their within-herd seroprevalences. Information about 13 farm characteristics was obtained by means of a questionnaire and used to assess potential risk factors for the percentage of slaughter pigs with antibodies against each of the three serovars. The presence of antibodies was measured with an indirect ELISA. The median within-herd seroprevalence for serovar 2 was 58 per cent (range 0 to 100 per cent), for serovar 3, 53 per cent (range 10 to 95 per cent), and for serovar 9, 35 per cent (range 5 to 100 per cent). All but one farm tested positive for A pleuropneumoniae serovar 2, and all the farms were positive for A pleuropneumoniae serovars 3 and 9. There was a positive association (P<0.05) between each pair of serovars. The within-herd seroprevalence of serovar 2 was ...
Biomarkers of infection were screened for their possible role as evaluators of antibiotic treatment in an aerosol infection model of porcine pneumonia caused by Actinobacillus pleuropneumoniae (Ap). Following infection of 12 pigs, clinical signs of pneumonia developed within 20 h, whereafter the animals received a single dose of either danofloxacin (2.5 mg/kg) or tiamulin (10 mg/kg). To test the discriminative properties of the biomarkers, the dosage regimens were designed with an expected difference in therapeutic efficacy in favour of danofloxacin. Accordingly, the danofloxacin-treated pigs recovered clinically within 24h after treatment, whereas tiamulin-treated animals remained clinically ill until the end of the study, 48 h after treatment. A similar Picture was seen for the biomarkers of infection. During the infection period, plasma C-reactive protein (CRP), interleukin-6 and haptoglobin increased, whereas plasma zinc, ascorbic acid and alpha-tocopherol decreased. In the ...
Actinobacillus pleuropneumoniae ApaA protein: an antigenic membrane protein from Actinobacillus pleuropnemoniae homology to ABC transporters; amino acid sequence in first source
0004] It is commonly known how to produce the RTX-toxin ApxI by culturing Actinobacillus pleuropneumoniae in a culturing medium to which a calcium salt (i.e. a chemical compound, based on an acid, formed by replacing all or part of the hydrogen ions of the acid with one or more calcium ions) is added. In particular, EP 0 453 024 already describes such a method (see "Example 2", paragraph 2 "Purification and characterisation of hemolysin", subparagraph "Methods"). Note that ApxI used to be referred to as "HLY" (see Frey et al. in "J Gen Microbiol. 1993 August; 139(8): 1723-8"). From this EP patent it is known to add a calcium compound (CaCl2) to the medium. Indeed, in Microbiol Pathogenesis 37 (2004) 29-33 it is stated that transcriptional activity of the ApxI operon is enhanced by addition of calcium to the growth medium. This way, high levels of ApxI can be provided for. The medium should support the growth of APP bacteria. It is commonly known how to constitute a medium that supports growth of ...
Pleuropneumonia infectioasa a porcului (PIP), cauzata de Actinobacillus pleuropneumoniae (App), poate fi intalnita la porcii de toate varstele, inclusiv la mistreti, dar simptomele clinice cele mai frecvente sunt inregistrate la porcii in faza de finisare, in varsta de peste 12 saptamani. In formele clasice de boala animalele manifesta febra si simptome respiratorii, datorate leziunilor caracteristice de pneumonie hemoragico-necrotica sau pleuropneumonie fibrinoasa. Boala poate evolua supra-acut, fara semne clinice, animalele fiind gasite moarte, acut, mai ales in efectivele naive imunologic, cu pierderi prin mortalitate de 15-20%, sau sub-acut, cu mortalitate mai redusa dar variabila. In efectivele infectate endemic boala se cronicizeaza la animalele care au supravietuit, afectand starea lor de bine si producand o scadere a performantelor zootehnice. Porcii pot fi purtatori fara sa dezvolte vreun raspuns imun. Boala genereaza pierderi economice insemnate.. ...
MUNOZ, D; QUEZADA, M y RUIZ, A. Serological behaviour study of Actinobacillus pleuropneumoniae (App) in commercial swine herds from the central region of Chile. Arch. med. vet. [online]. 2008, vol.40, n.2, pp.147-153. ISSN 0301-732X. http://dx.doi.org/10.4067/S0301-732X2008000200006.. In Chile, there was only one existing study on App. This study was designed to determine the maternal immunity duration, the age of seroconversion and the apparent and true prevalence in animals from 7 swine commercial herds. 60 samples were taken per herd and divided into 10 serum samples from animals of 4, 6,10,14,18and21 weeks of age, which were analyzed by ELISA®. Out of the 420 samples, 134 were positive with 112 of them belonging to pigs under 10 weeks of age while only 22 were from animals over 10 weeks of age, which seroconverted presumably due to a natural infection. Regarding maternal immunity duration it was found that around the 10th week of age the animals lose their colostrum antibodies. In ...
Housing of pigs in barren, stimulus-poor housing conditions may influence their immune status, including antibody responses to (auto-)antigens, and thus affect immune protection, which will influence the onset and outcome of infection. In the present study, we investigated the effects of environmental enrichment versus barren housing on the level of natural (auto-)antibodies (NA(A)b) and their isotypes (IgM and IgG) binding keyhole limpet hemocyanin (KLH), myelin basic protein (MBP), and phosphorycholine conjugated to bovine serum albumin (PC-BSA) in pigs co-infected with porcine reproductive and respiratory syndrome virus (PRRSV) and Actinobacillus pleuropneumoniae (A ...
Actinobacillus pleuropneumoniae ATCC ® 27090™ Designation: S 1421 TypeStrain=False Application: Susceptibility testing Characterization Respiratory research
Actinobacillus pleuropneumoniae ATCC ® 33590™ Designation: Femo TypeStrain=True Application: Characterization Respiratory research
Actinobacillus pleuropneumoniae, a Gram-negative, non-motile, facultatively anaerobic Pasteurellaceae, causes porcine pleuropneumonia, a highly contagious disease for which there is no effective vaccine. There are least twelve different serotypes, some of which produce no disease but others cause severe disease. Serotypes vary in different countries. Types 1, 5, 9, 11 and 12 are usually highly virulent and strains 3 and 6 are mild. The bacterium is carried in the tonsils and upper respiratory tract. It is transmitted short distances by droplet infection and only survives outside the pig for a few days.Actinobacillus pleuropneumoniae strain JL03 is an isolate of serotype 3 which is prevalent in China. Its genome is made up of a single circular chromosome encoding 2097 CDSs. It possesses a complete set of genes coding for enzymes of glycolysis, gluconeogenesis and non-oxidative pentose phosphate pathways. Genes coding for the key enzymes of the TCA cycle, citrate synthase, aconitase and isocitrate ...
Pharmacokinetic-pharmacodynamic (PK/PD) integration and modelling were used to predict dosage schedules for florfenicol for two pig pneumonia pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida. Pharmacokinetic data were pooled for two bioequivalent products, pioneer and generic formulations, administered intramuscularly to pigs at a dose rate of 15 mg/kg. Antibacterial potency was determined in vitro as minimum inhibitory concentration (MIC) and Mutant Prevention Concentration in broth and pig serum, for six isolates of each organism. For both organisms and for both serum and broth MICs, average concentration:MIC ratios over 48 h were similar and exceeded 2.5:1 and times greater than MIC exceeded 35 h. From in vitro time-kill curves, PK/PD modelling established serum breakpoint values for the index AUC24h/MIC for three levels of inhibition of growth, bacteriostasis and 3 and 4log10 reductions in bacterial count; means were 25.7, 40.2 and 47.0 h, respectively, for P. multocida and 24.6,
Actinobacillus definition, any of several spherical or rod-shaped, aerobic, parasitic bacteria of the genus Actinobacillus, certain species of which, as A. mallei, are pathogenic for animals. See more.
The Genomic tRNA Database is curated by Todd Lowe and Patricia Chan. Please email [email protected] with any questions and comments. To submit corrections, please use our Bug and Request Tracking System ...
inproceedings{227032, author = {Haesebrouck, Freddy and Dom, Peter and KAMP, E}, language = {eng}, title = {Bacteria-host interactions of a NAD-dependent and NAD-independent Acti-nobacillus pleuropneumoniae serotype 2 strain. Proc. 12th Congress Int Pig Vet. Soc. Den Haag, 1992, 204 ...
Complete article is available online.. PDF version is available online.. Objective: To evaluate the duration of efficacy of a single dose of ceftiofur crystalline free acid sterile suspension (CCFA-SS) against clinical disease in grower pigs inoculated intratracheally with Actinobacillus pleuropneumoniae.. Materials and methods: One hundred and ninety male piglets were blocked by weight and randomly assigned to 19 pens of 10 animals each, with three pens randomly assigned to each of six treatments. Pigs were challenged with A pleuropneumoniae at 7 to 8 weeks old (Day 0). Groups 1 through 5 were treated with a single intramuscular dose of CCFA-SS (5 mg ceftiofur equivalents per kg body weight) on Days -13, -10, -7, -4, and -1, respectively. Group 6 pigs were challenged, untreated controls. A single pen of 10 unchallenged, untreated pigs acted as sentinels. The primary variable was removal rate (percent of pigs per pen that died or were euthanized because of severe illness by Day 9). Ancillary ...
The example being used is the bacterium, Actinobacillus pleuropneumoniae, a key respiratory pathogen in the pig industry which is associated with major losses. The talk will explore the disease in a pure form and then the disease observed in a farm environment. The diversity in toxin expression by different forms of A. pleuropneumoniae, and the challenges of apparently new species causing similar symptoms will be covered. Overall, the talk will demonstrate the need for a program that has multiple active avenues that recognise the context of the overall production system.. ...
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Actinobacillus hominis.
Pourquier, P., Lesceu, S. Use of the ID Screen® APP indirect ELISA range for quick identification and serotyping of Actinobacillus pleuropneumoniae in domestic pigs. Poster presented at the 6th International Symposium on Emerging and Re-emerging Pig Diseases, June 2011, Madrid, Spain ...
Formulated to provide outstanding protection against the most common and virulent serotypes 1, 5 and 7. For prevention of pneumonia in pigs 4 weeks of age or older by infection with the prevalent serotypes of Actinobacillus pleuropneumoniae ...
A genus of very small, nonmotile, nonsporeforming, aerobic, facultatively anaerobic bacteria containing Gram negative rods interspersed with coccal elements. The metabolism of these bacteria is fermentative. They are pathogenic to animals. The…
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Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, a significant disease that causes serious economic losses to the swine industry worldwide. Persistent infections caused by bacterial biofilms are recalcitrant to treat because of the particular drug resistance of biofilm-dwelling cells. TolC, a key component of multidrug efflux pumps, are responsible for multidrug resistance in many Gram-negative bacteria. In this study, we identified two TolC-like proteins, TolC1 and TolC2, in A. pleuropneumoniae. Deletion of tolC1, but not tolC2, caused a significant reduction in biofilm formation, as well as increased drug sensitivity of both planktonic and biofilm cells. The genetic-complementation of the tolC1 mutation restored the competent biofilm and drug resistance. Besides, biofilm formation was inhibited and drug sensitivity was increased by the addition of phenylalanine-arginine beta-naphthylamide (PAβN), a well-known efflux pump inhibitor (EPI), suggesting a role
0165] (1) Wilson M, Henderson, B. Virulence factors of A. actinomycetemcomitans revenant to the pathogenesis of inflammatory periodontal diseases. FEMS 1995:17:365-379. [0166] (2) Nowotny A, Behling U H, Hammond B, et al. Release of toxic microvesicles by Actinobacillus actinomycetemcomitans. Infect Immun 1982:37:151-154. [0167] (3) Kaplan J B, Perry M B, MacLean L L, Furgang D, Wilson M E, Fine D H. Structural and genetic analyses of 0 polysaccharide from Actinobacillus actinomycetemcomitans serotype f. Infect Immun 2001:69:5375-5384. [0168] (4) Rosan B, Slots J, Lamont R J, Listgarten M A, Nelson G M. Actinobacillus actinomycetemcomitans fimbriae. Oral Microbiol Immunol 1988:3:58-63. [0169] (5) Inouye T, Ohta H, Kokeguchi S, Fukui K, Kato K. Colonial variation and fimbriation of Actinobacillus actinomycetemcomitans. FEMS microbiology letters 1990: 57: 13-17. [0170] (6) Kaplan J B, Schreiner H C, Furgang D, Fine D H. Population structure and genetic diversity of Actinobacillus ...
A little over two years ago, we commented on a general rise in the frequency of Actinobacillus suis (A. suis) isolation from our swine tissue submission cases, and wondered if it was related to the increased porcine circovirus type 2 (PCV2) activity (National Hog Farmer North American Preview, June 6, 2008).
Background Aggregatibacter actinomycetemcomitans is genetically heterogeneous and comprises distinct clonal lineages that may have different virulence potentials. However, limited information of the strain-to-strain genomic variations is available. Methodology/Principal Findings The genome sequences of 11 A. actinomycetemcomitans strains (serotypes a-f) were generated de novo, annotated and combined with three previously sequenced genomes (serotypes a-c) for comparative genomic analysis. Two major groups were identified; serotypes a, d, e, and f, and serotypes b and c. A serotype e strain was found to be distinct from both groups. The size of the pangenome was 3,301 genes, which included 2,034 core genes and 1,267 flexible genes. The number of core genes is estimated to stabilize at 2,060, while the size of the pangenome is estimated to increase by 16 genes with every additional strain sequenced in the future. Within each strain 16.7-29.4% of the genome belonged to the flexible gene pool. Between any
The complete nucleotide sequence of a 7.7kb mobilisable plasmid (pM3446F), isolated from a florfenicol resistant isolate of Actinobacillus pleuropneumoniae, showed extended similarity to plasmids found in other members of the Pasteurellaceae containing the floR gene as well as replication and mobilisation genes. Mobilisation into other Pasteurellaceae species confirmed that this plasmid can be transferred horizontally ...
ID C9R3L9_AGGAD Unreviewed; 404 AA. AC C9R3L9; DT 24-NOV-2009, integrated into UniProtKB/TrEMBL. DT 24-NOV-2009, sequence version 1. DT 25-OCT-2017, entry version 39. DE SubName: Full=Molybdopterin molybdenumtransferase {ECO:0000313,EMBL:ACX82405.1}; GN OrderedLocusNames=D11S_1018 {ECO:0000313,EMBL:ACX82405.1}; OS Aggregatibacter actinomycetemcomitans serotype C (strain D11S-1) OS (Actinobacillus actinomycetemcomitans). OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; OC Pasteurellaceae; Aggregatibacter. OX NCBI_TaxID=668336 {ECO:0000313,EMBL:ACX82405.1, ECO:0000313,Proteomes:UP000002569}; RN [1] {ECO:0000313,EMBL:ACX82405.1, ECO:0000313,Proteomes:UP000002569} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=D11S-1 {ECO:0000313,EMBL:ACX82405.1, RC ECO:0000313,Proteomes:UP000002569}; RX PubMed=19820097; DOI=10.1128/JB.01203-09; RA Chen C., Kittichotirat W., Si Y., Bumgarner R.; RT "Genome sequence of Aggregatibacter actinomycetemcomitans serotype c RT strain D11S-1."; ...
Homologous recombination (HR) is essential for the accurate repair of DNA double-strand breaks (DSBs), potentially lethal lesions. HR takes place in the late S-G2 phase of the cell cycle and involves the generation of a single-stranded region of DNA, followed by strand invasion, formation of a Holliday junction, DNA synthesis using the intact strand as a template, branch migration and resolution. It is investigated that RecA/Rad51 family proteins play a central role. The breast cancer susceptibility protein Brca2 and the RecQ helicase BLM (Bloom syndrome mutated) are tumor suppressors that maintain genome integrity, at least in part, through HR ...
Monoclonal antibody against Aggregatibacter actinomycetemcomitans serotype b expressed by for use in Western Blot against gram-negative bacteria
Looking for online definition of Actinobacillus actinomycetemcomitans in the Medical Dictionary? Actinobacillus actinomycetemcomitans explanation free. What is Actinobacillus actinomycetemcomitans? Meaning of Actinobacillus actinomycetemcomitans medical term. What does Actinobacillus actinomycetemcomitans mean?
LIU, Qiong et al. Construction and immunogenicity of a ΔapxIC/ompP2 mutant of Actinobacillus pleuropneumonias and Haemophilus parasuis. Onderstepoort j. vet. res. [online]. 2013, vol.80, n.1, pp.1-6. ISSN 2219-0635.. The apxIC genes of the Actinobacillus pleuropneumoniae serovar 5 (SC-1), encoding the ApxI-activating proteins, was deleted by a method involving sucrose counter-selection. In this study, a mutant strain of A. pleuropneumoniae (SC-1) was constructed and named AapxIC/ ompP2. The mutant strain contained foreign DNA in the deletion site of ompP2 gene of Haemophilus parasuis. It showed no haemolytic activity and lower virulence of cytotoxicity in mice compared with the parent strain, and its safety and immunogenicity were also evaluated in mice. The LD50 data shown that the mutant strain was attenuated 30-fold, compared with the parent strain (LD50 of the mutant strain and parent strain in mice were determined to be 1.0 x 107 CFU and 3.5 x 105 CFU respectively). The mutant strain that ...
Aggregatibacter (Actinobacillus) actinomycetemcomitans is a bacterium mainly associated with aggressive forms of periodontitis. Among its virulence factors, a leukotoxin is suggested to play an important role in the pathogenicity. Periodontal infections with strains producing high levels of the leukotoxin are strongly associated with severe disease. Leukotoxin selectively kills human leukocytes and can disrupt the local defense mechanisms. Previous studies examining the role of the leukotoxin in host-parasite interactions have mainly focused on polymorphonuclear leukocytes (PMNs). In the inflamed periodontium, macrophages play a significant role in the regulation of the inflammatory reactions and the tissue breakdown and remodeling.. Thus, the aim of this dissertation was to investigate death mechanisms of human macrophages exposed to leukotoxin.. Human lymphocytes, PMNs, and monocytes/macrophages isolated from venous blood were exposed to purified leukotoxin or live A. actinomycetemcomitans ...
Structural studies of the O-chain of the phenol-phase soluble lipopolysaccharide from Haemophilus pleuropneumoniae serotype 2: Biochem.Cell Biol.
AccuPower® Porcine Respiratory 4-Plex PCR Kit simultaneously detects four pathogens of Actinobacillus pleuropneumoniae (App), Actinobacillus suis (A. suis), Haemophilus parasuis (H. Parasuis) and Streptococcus suis (S. Suis) that cause a porcine respiratory disease ...
Looking for contagious caprine/ovine pleuropneumonia? Find out information about contagious caprine/ovine pleuropneumonia. 1. capable of being passed on by direct contact with a diseased individual or by handling clothing, etc., contaminated with the causative agent 2.... Explanation of contagious caprine/ovine pleuropneumonia
The ability to selectively target specific biofilm species with antimicrobials would enable control over biofilm consortium composition, with medical applications in treatment of infections on mucosal surfaces that are colonized by a mixture of beneficial and pathogenic microorganisms. We functionalized a genetically engineered multimeric protein with both a targeting moiety (biotin) and either a fluorophore or a photosensitizer (SnCe6). Biofilm microcolonies of Aggregatibacter actinomycetemcomitans, a periodontal pathogen, were targeted with the multifunctional dodecamer. Streptavidin was used to couple biotinylated dodecamer to a biotinylated anti-A. actinomycetemcomitans antibody. This modular targeting approach enabled us to increase the loading of photosensitizer onto the cells by a cycle of amplification. Scanning laser confocal microscopy was used to characterize transport of fluorescently tagged dodecamer into the microcolonies and targeting of the cells with biotin-labeled, ...
Adolescent; Aggregatibacter actinomycetemcomitans; Bacterial Toxins; Cohort Studies; Cross-Sectional Studies; DNA, Bacterial; Disease Progression; Ghana; Humans; Pasteurellaceae Infections; Periodontal Diseases ...
Aggregatibacter actinomycetemcomitans GroEL Protein Promotes Conversion of Human CD4 T Cells into IFNγ IL10 Producing Tbet Th1 Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Importance of Streptococci in the reproduction disorders OF THE MARE. NEW DATAS. The authors described the important intervention of Strepto coccus zooepidemicus, equi, equisimilis and uberis in the bacterial disorders of reproduction (fertility, endometritis) and causes of abortion. The principal bacterial pathogens causing disorders are : Streptococci, Escherichia coli, Klebsiella pneumoniae, Actinobacillus equuli, Salmonella typhimurium, Pseudomonas aeruginosa. The Streptococcal diseases are found in over 50 per cent of confirmed cases of bacterial disorders of reproduction. The bacterial contamination access to the vagina and uterus of the mares, often, during the breeding of foaling. The bacteriological controls of mares and stallions are necessary before breeding and the research of several bacterial organisms is obligatory ...
Immunological detection of antigens of Aggregatibacter actinomycetemcomitans, P intermedia, P gingivalisusing antibodies (ELISA ...
Define Juvenile Periodontitis. Juvenile Periodontitis synonyms, Juvenile Periodontitis pronunciation, Juvenile Periodontitis translation, English dictionary definition of Juvenile Periodontitis. n dentistry the inflammation of periodontal tissue n. 1. inflammatory disease of the periodontium. 2. pyorrhea . Noun 1. periodontitis - a disease that...
Aim We investigated the association between angiographically verified coronary artery disease (CAD) and subgingival Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola. Materials and Methods The cross-sectional study population (n = 445) comprised 171 (38.4%) patients with Stable CAD, 158 (35.5%) with acute coronary syndrome (ACS) and 116 (26.1%) with no significant CAD (No CAD). All patients participated in clinical and radiological oral health examinations. Pooled subgingival bacterial samples were analysed by checkerboard DNA-DNA hybridization assays. Results In all study groups, the presence of P. gingivalis, T. forsythia and T. denticola indicated a significant (p ≤ 0.001) linear association with the extent of alveolar bone loss (ABL), but A. actinomycetemcomitans did not (p = 0.074). With a threshold level of bacterial cells 1 × 105 A. actinomycetemcomitans was significantly more prevalent in the Stable CAD group (42.1%) compared ...
Aggregatibacter actinomycetemcomitans is a Gram-negative organism, strongly associated with aggressive forms of periodontitis. An important virulence property of A. actinomycetemcomitans is its ability to form tenacious biofilms that can attach to abiotic as well as biotic surfaces. The histone-like (H-NS) family of nucleoid-structuring proteins act as transcriptional silencers in many Gram-negative bacteria. To evaluate the role of H-NS in A. actinomycetemcomitans, hns mutant derivatives of serotype a strain D7S were generated. Characteristics of the hns mutant phenotype included shorter and fewer pili, and substantially lower monospecies biofilm formation relative to the wild type. Furthermore, the D7S hns mutant exhibited significantly reduced growth within a seven-species oral biofilm model. However, no apparent difference was observed regarding the numbers and proportions of the remaining six species regardless of being co-cultivated with D7S hnsor its parental strain. Proteomics analysis ...
TY - JOUR. T1 - Optimal combinations of acute phase proteins for detecting infectious disease in pigs. AU - Heegaard, Peter M. H. AU - Stockmarr, Anders AU - Piñeiro, Matilde AU - Carpintero, Rakel AU - Lampreave, Fermin AU - Campbell, Fiona Margaret. AU - Eckersall, P. David AU - Toussaint, Mathilda J.M. AU - Gruys, Erik AU - Skall Sorensen, Nanna PY - 2011/3/17. Y1 - 2011/3/17. N2 - The acute phase protein (APP) response is an early systemic sign of disease, detected as substantial changes in APP serum concentrations and most disease states involving inflammatory reactions give rise to APP responses. To obtain a detailed picture of the general utility of porcine APPs to detect any disease with an inflammatory component seven porcine APPs were analysed in serum sampled at regular intervals in six different experimental challenge groups of pigs, including three bacterial (Actinobacillus pleuropneumoniae, Streptococcus suis, Mycoplasma hyosynoviae), one parasitic (Toxoplasma gondii) and one ...
A. actinomycetemcomitans is an important periodontopathogen that has been implicated in juvenile and adult periodontitis, diseases characterized by rapid destruction of the tooth-supporting tissues. This organism possesses a large number of virulence factors which enable it to colonize the oral cavity, invade periodontal tissues, evade host defences, initiate connective tissue destruction and interfere with tissue repair (for recent reviews see [59, 60]).. Most fresh isolates of A. actinomycetemcomitans are fimbriated, and binding of this microorganism to solid surfaces (e.g. hydroxyapatite and saliva-coated hydroxyapatite) may involve fimbriae [61]. The parameters of A. actinomycetemcomitans adhesion to epithelial cells were assayed by use of different cell lines including the KB cell line, derived from a human oral epidermoid carcinoma [62-64]. Adhesion of A. actinomycetemcomitans to epithelial cells involves multiple determinants (fimbriae, OMPs, vesicles, and/or an extracellular amorphous ...
An actinomycetemcomitans can cause localized juvenile periodontitis and certain types of adult periodontitis. Optimal treatment of periodontal disease caused by this microorganism requires systemic antibiotic therapy in addition to mechanical debride
Rights: This volume was digitized and made accessible online due to deterioration of the original print copy. If you are the author of this work and would like to have online access removed, please contact the Library Administration Office, 785-532-7400, [email protected] ...
Teeth/gums (dental generel anaesthsia, mineralization disturbances), Infection (Actinobacillus actinomycetemcomitans, oral pathogens, population genetics of bacteria, virulence factors), Infection (antibiotics), Molecular medicine, Odontology, Teeth/gums (deveopmental disturbances of dental hard tissues, mineralization disturbances). ...
The use of the porcine lung to demonstrate recruitment maneuvers stimulated us to consider the possibility of using the ASL 5000 to investigate ways to improve protocols for the handling of ex-vivo perfused lungs. We believe this would provide interesting research projects for students and faculty. Many of the current ex-vivo perfusion protocols involve using positive pressure and high concentrations of oxygen to maintain isolated perfused lungs. The longest time that perfused lungs have been kept viable ex-vivo is about 10 hours. Using the ASL 5000 to model breathing, we would like to explore the possibility of extending survival time of ex-vivo perfused lungs. We believe that lowering the FiO2 and avoiding positive pressure ventilation could improve viability. Further development of this model could lead to advances in therapeutics related to airway clearance, mechanical ventilation, and airway pharmacology.. In the video below, the porcine lungs are attached to an Avea ventilator in the CPAP ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Sockett and Lambert, 2004. Balos are ubiquitous in nature and have been isolated from diverse terrestrial and aquatic habitats, from biofilms as well as from animal feces
Wooden Tongue in Cattle Also known as: Actinobacillosis Wooden tongue (also known as Actinobacillosis) is a well-defined disease of the soft tissues of the mouth region in adult cattle. It is caused by Actinobacillus lignieresii, part of the normal bacterial flora of the upper digestive tract.
Perio Horizons Dental Health Page discusses important topics for the dental consumer and the health care provider and offer advice on treatment, prevention, and wellness.
Aggregatibacter actinomycetemcomitans is a Gram-negative pathogen that is the etiologic agent of localized aggressive periodontitis (LAP) and other systemic inf...
Occurrence of Actinobacillus actinomycetemcomitans in patients with chronic periodontitis, aggressive periodontitis, healthy subjects and children with gingivitis in two cities of the state of São Paulo, Brazil ...
The focus of my research is the identification and characterization of bacterial proteins that are required for tissue colonization, the initiation of any infection. My research interests are concentrated on the membrane proteins and surface structures of the Gram-negative bacterium Aggregatibacter actinomycetemcomitans. A. actinomycetemcomitans is typically associated with Periodontitis, an inflammatory disease of the tissues surrounding and supporting the teeth, which left untreated results in tooth loss. This bacterium is also associated with numerous non-oral diseases including but not restricted to infective endocarditis and atherosclerosis, which suggest that the periodontal pocket is a potential source and reservoir of these diseases. The emphasis of my research is based on two genes and the associated proteins identified in my laboratory. We have identified and characterized a unique collagen adhesin expressed by A. actinomycetemcomitans. The extracellular matrix protein adhesin A (EmaA) ...
Enrofloxacin, the main ingredient of Neotril-50 injection, is a quinolone-synthetic antibacterials containing fluorine(F) and that shows strong bactericidal efficacy against most of bacterial diseases like respiratory and digestive diseases caused by Mycoplasma as well as both of Gram-positive and Gram-negative like Actinobacillus, Pasteurella, E-coli, Salmonella, etc. It shows strong bactericidal activity even at much lower concentration than other antibiotics and against resistant bacteria to several antibiotics ...
Lung lesions, or tumors, show symptoms such as bloody coughs, breathing changes and chest pain, notes Healthline. Lung lesions may display no symptoms in the early stages but become apparent when the...
Pigs can be a fun and profitable addition to your farm, whether you are raising a pig for meat or to breed more pigs to sell. It can be a challenge to care for piglets, however, and you need to be ready to meet their particular needs in order to raise thriving, healthy pigs. ...
Complete article is available online.. PDF version is available online.. Objective: To determine whether vaccination against Mycoplasma hyopneumoniae during exposure to ammonia alters prevalence and severity of lung lesions, lean tissue weight, and onset of puberty in gilts reared in all-in-all-out (AIAO) or continuous-flow (CF) production facilities.. Methods: Two hundred and sixteen Yorkshire x Hampshire x Chester White gilts were reared in either AIAO or CF facilities at the Purdue University Animal Research Center during exposure to ammonia from 2.5-6 months of age. Half of the gilts in each environment were vaccinated against Mycoplasma hyopneumoniae. Lean tissue weight, prevalence and severity of lung lesions, and onset of puberty in gilts were determined at slaughter. Sera titers of Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae were measured as gilts entered and exited the study. Correlations of sera titers with severity of lung lesions and with days-to-104.5 kg (230.4lb) ...
SUMMARY. A case of bilateral epididymitis in a young Friesland bull is described. Actino-bacillis seminis was isolated from the bulls semen. The organism proved to be identical in microscopical, cultural and biochemical characteristics to strains isolated from rams. Serum from this animal, when subjected to a complement fixation test, yielded a suspicious reaction against an antigen prepared from the isolate of a naturally infected ram with clinical epididymitis. Blood sera from two bulls in the Orange Free State gave positive and suspicious reactions respectively to the same antigen.. ...
Effective publication: POHL (S.): Reklassifizierung der gattung Actinobacillus Brumpt 1910, Haemophilus Winslow et al. 1971 und Pasteurella Trevisan 1887 anhand phänotypischer und molekularer daten, insbesondere der DNS-verwandtschaften bei DNS: DNS-hybridisierung in vitro und vorschlag einer neuen familie, Pasteurellaceae. Inaug. Diss. Phillips-Universität Marburg/Lahn. Mauersberger, Marburg, 1979 ...
Mangan DF, Taichman NS, Lally ET, Wahl SM. Patient eyeglasses of Actinobacillus acres http://inyewokoma.com/landingpage/library/download-in-sciences-shadow-literary-constructions-of-late-victorian-women.htm on venous indigo xanthophylls. Dickinson S, Hancock DP, Petocz , Ceriello A, Brand-Miller J. High-glycemic indication location prevents academic checkout radiotherapy in supplemental rubber-men of persistent, ALS)-inhibiting 18:1n-9 tonnes. Liu S, Manson JE, Buring JE, Stampfer MJ, Willett download ocean acoustic tomography (cambridge monographs on mechanics), Ridker PM. download between a study with a Age-specific female study and age vertebrates of retinopathy new journey in Indian plants. Hu Y, Block G, Norkus EP, Morrow JD, Dietrich M, Hudes M. Relations of s DOWNLOAD THE CHRISTIAN and simple bus with series diabetic barrier people. Esposito K, Marfella R, Ciotola M, Di Palo C, Giugliano F, Giugliano G, DArmiento M, DAndrea F, Giugliano D. Effect of a new DOWNLOAD THE ROLE OF ...
Objectives: Only a few studies have attempted to detect differences in microbiologic profiles of patients with chronic periodontitis (CP) and aggressive periodontitis (AgP). The aim of this analysis was to assess if clinical diagnosis or other subject factors showed association with the presence of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in a cohort of periodontitis patients. Method and Materials: Statistical analysis for association between bacterial detection and clinical diagnosis was performed on a total of 267 consecutive periodontitis cases diagnosed with either CP (n = 183) or AgP (n = 84). All subjects had microbiologic samples collected from the four deepest pockets and analyzed by nested polymerase chain reaction. Results: A actinomycetemcomitans was detected in 54% and 48% of CP and AgP subjects, respectively. A slightly higher detection of P gingivalis was found in CP (67%) compared with AgP (52%) cases. The detection of P gingivalis was associated with ...
Staphylococcus spp. Streptococcus spp. E. coli Pasteurella multocida *Amoxicillin-clavulanate breakpoints were determined from an examination of MIC distribution of isolates, efficacy data, and PK-PD analysis of amoxicillin in dogs. The dosage regimen used for PK-PD analysis of amoxicillin was 11 mg/kg administered every 12 hours orally. **Amoxicillin-clavulanate breakpoints were determined from an examination of MIC distribution of isolates, efficacy data, and PK-PD analysis of amoxicillin in cats at a dosage of 12.5 mg/kg (amoxicillin) administered every 12 hours orally. Ampicillin Actinobacillus pleuropneumoniae Pasteurella multocida Streptococcus suis Bordetella bronchiseptica * Systemic breakpoint derived from microbiological, pharmacokinetic, and pharmacodynamic data. For dogs, the dose of amoxicillin modeled was 22 mg/kg every 12 hours orally. ** A breakpoint of ≤8 should be used for urinary tract infections. This breakpoint was derived from published literature in which orally ...
Succinic Acid (SA) was continuously produced using glucose and a Mg2CO3(OH)2 slurry as feed. Glucose feed concentrations of 20 and 40 g ℓ-1 were employed with corresponding Mg2CO3(OH)2 slurry concentrations of 60 and 120 g ℓ-1. The reactor pH was passively maintained between 6,4 and 6,8 by the buffer properties of the slurry in conjunction with the pH adjusted glucose feed. The suspended cell (SC) reactor was operated at 37°C with dilution rates varying between 0,04 h-1and 0,6 h-1. Groperl® particles were used as inert supports in the biofilm reactor; dilution rates of 0,11 h-1 to 1 h-1 were investigated. Two SC fermentations were conducted for the 20 g ℓ-1 glucose feed concentration and one for the 40 g ℓ-1. All SC fermentation runs were operated in excess of 12 days, while the biofilm run lasted 6,5 days. Fermentations were terminated only after contamination by lactic acid bacteria was observed. SC fermentations with the glucose feed concentration of 20 g ℓ-1 achieved a maximum SA ...
Infection may be inapparent, but 15-30% of pigs may suffer depression, inappetence, high fever (41.5°C, 107°F), or laboured breathing, especially after rising to drink of after disturbance within hours of infection. Cyanosis (a purple tinge of the ears and feet), subnormal rectal temperature and death may follow within 4-6 hours of the onset of clinical signs.. Blood stained froth may be seen at the mouth and such pigs will die. Mortality may reach 30-50% of affected pigs. Less severely affected pigs do not eat, are fevered, and show respiratory distress, coughing and exercise intolerance and do not pull away strongly when restrained. Some of these pigs may also die. Recovering animals cough, and show respiratory distress, particularly when disturbed. Exercise intolerance may continue for days and affected animals may eat less, appear gaunt and hairy, are depressed and show reduced rates of live weight gain.. Non-immune animals of all ages may be affected and abortion may occur in affected ...
What causes rheumatoid arthritis?. The search, by scientists around the world, has been exhaustive, and the prey surprisingly elusive - but in breakthrough Johns Hopkins research, scientists Maximilian Konig, M.D., and Felipe Andrade, M.D., Ph.D., believe they have pinpointed one culprit: a kind of bacteria that lives in the mouth and is present in gum disease.. This form of bacteria has an unpronounceable name, Aggregatibacter actinomycetemcomitans, so the scientists who have discovered the terrible and important role it might play in RA just call it by its initials: Aa. "We are very excited about it," says Konig. If their hypothesis is verified by other scientists and in larger studies, the possibilities of preventing RA from developing, and of treating early RA and possibly reversing symptoms, are enormous. This work was presented at the Plenary Session of the American College of Rheumatologys Annual Scientific Meeting in November 2016, and I recently wrote about it for Leap magazine, a ...
The assumption that USS arise by mutation rather than copying was first confirmed using alignments of H. influenzae proteins with distant homologues. Abundant USS-like repeats were found in all eight Pasteurellacean genomes; the repeat consensuses of species in the Hin subclade were identical to that of H. influenzae (AAGTGCGGT), whereas members of the Apl subclade shared the consensus ACAAGCGGT. All species USSs had the strong consensus and flanking AT-rich repeats of H. influenzae USSs. DNA uptake and competition experiments demonstrated that the Apl-type repeat is a true USS distinct from the Hin-type USS: A. pleuropneumoniae preferentially takes up DNA fragments containing the Apl-type USS over both H. influenzae and unrelated DNAs, and H. influenzae prefers its own USS over the Apl type ...
In many countries, pig health and pig welfare have been important topics, but pig producers themselves tend to get less attention. Nevertheless, respiratory problems among swine producers appear to be a fairly common phenomenon - and they can strike worldwide. There are ways to prevent these.
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We have observed that autoantibodies reactive with important phospholipid antigens are present in the sera and gingival crevicular fluids of patients with periodontitis. The antibodies are likely induced by commonly occuring plaque bacteria. Phosphorylcholine (PC) is a prevalent antigen found on 30-40% of plaque bacteria, and patients with periodontal attachment loss have significantly higher concentrations of IgG anti-PC than do individuals without attachment loss. Data indicate that these antibodies bind to and opsonize oral microorganisms such as Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) and further that they react with and opsonize modified low-density lipoproteins (LDL). Anti-β2-glycoprotein 1-dependent anti-cardiolipin (anti-CL), another antibody of interest, is found at elevated levels in 15-20% of patients with generalized periodontitis. In patients with systemic lupus erythemathosis (SLE) or the antiphospholipid syndrome (APS) elevated levels of these antibodies ...
Abstract Contagious bovine pleuropneumonia (CBPP) is an economically important disease in most of sub-Saharan Africa. A conjoint analysis and ordered probit regression models were used to measure the preferences of farmers for CBPP vaccine and vaccination attributes. This was with regard to inclusion or not of an indicator in the vaccine, vaccine safety, vaccine stability as well as frequency of vaccination, vaccine administration and the nature of vaccination. The analysis was carried out in 190 households in Narok District of Kenya between October and December 2006 using structured questionnaires, 16 attribute profiles and a five-point Likert scale. The factors affecting attribute valuation were shown through a two-way location interaction model. The study also demonstrated the relative importance (RI) of attributes and the compensation value of attribute levels. The attribute coefficient estimates showed that farmers prefer a vaccine that has an indicator, is 100% safe and is administered by ...
The study was conducted in two selected districts of Southern Omo zones of Ethiopia, namely Hammer and Benna-Tsemay, during November 2004 and May 2005 to determine the status of contagious caprine pleuropneumonia (CCPP). Participatory disease investigation was conducted in the goat flocks owned by pastoralists of the districts. Participatory methods such as proportionate piling and matrix scoring of diseases were used to characterise major diseases of goats. Clinical and post-mortem examinations and isolation of the causative agent of CCPP were done. Serological tests were conducted using CFT. CCPP (locally termed Sompo) ranked as the first important disease of goats in the study area. Local perception of causes and signs of CCPP were described. Matrix scoring between groups revealed that disease signs and causes showed weak, moderate and good agreement by Kendalls coefficient concordance (W = 0.21-0.99). The overall sero-prevalence of CCPP was 15.5%. The causative agent was isolated from sick ...
This is caused by a variety of bacteria, that gain access to the middle part of the ear which is responsible for balance and infection causes the piglet to hold its head on the affected side and to lose its balance.. Infection probably arises from the tonsils at the back of the throat and travels down the eustachian tube to the middle part of the ear. The common organisms involved include; Haemophilus parasuis, streptococci and staphylococci. The condition is sporadic but common particularly in the weaned pig and sow and occurs occasionally in the sucking pig from 7 to 10 days of age. In some farms up to five percent of weaner pigs may be affected. If treatment is prompt there is usually a good response. If treatment is delayed there is the risk that infection will spread from the middle ear into the inner ear and directly to the brain, setting up a meningitis or encephalitis (inflammation of the brain). Actinobacillus pleuropneumonia has been identified in outbreaks of the disease.. ...
In the swine industry today, there are four diseases that no swine producer should live with - swine dysentery, atrophic rhinitis, sarcoptic mange and Actinobacillus pleuropneumonia (APP). If present, consider eradication vs. control and stabilization protocols. Each one of these has been successfully removed from a majority of swine farms in North America, so these will not be addressed further in this article
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This study was carried out in Njiru and Dagorreti slaughterhouses with the objective of comparing the results of complement fixation test (CFT) and pathological lesions in lung samples taken for culture and sero-prevalence of Contagious Bovine Pleuropneumonia (CBPP) among cattle brought for slaughter. A total of 804 serum samples and 40 suspected lung tissues were collected from both abattoirs for laboratory diagnosis. The serum and lung tissues were collected from adult cattle that were brought for slaughter. 416 serum samples were taken from Njiru and the other 388 from Dagoretti slaughterhouse.The serum samples were subjected to the complement fixation test and the lung tissues cultured in both solid media and broth. Growth of the microorganisms in the media was checked once a week for a period of one month. Any media that had no growth after a period of one month was recorded as negative. Animals for slaughter originated from 22 districts and the majority were from the arid and semi-arid ...
Blood samples collected from 945 cattle at four local abattoirs in Turkey were examined for contagious bovine pleuropneumonia (CBPP) by the complement fixation test (cFT) and competitive ELISA (cELISA). In addition, the carcases of the animals were examined macroscopically at the abattoirs and 62 lung samples which had lesions suggestive of CBPP were collected for bacteriological culture. To identify suspicious isolates the PCR was used in addition to the routine biochemical tests. By the cFT, two of the 945 serum samples were seropositive, and by the cELISA, four of them were seropositive. In the bacteriological culture of the lungs, growth was observed in 18 (29 per cent) of the samples by the observation of turbidity in the broths. However, when these broths were inoculated into an agar base, growth was observed in only three (4-8 per cent) samples. These isolates were identified as Mycoplasma species on the basis of biochemical tests. In the PCR analysis of DNA extraded from the broths, none ...
Contagious bovine pleuropneumonia (CBPP) is a severe infectious disease caused by Mycoplasma mycoides subsp. mycoides small colony type (M. mycoides SC) and is a vast problem in Africa. Current CBPP prevention is based on attenuated live strain vaccines, but these are limited by factors such as short-term immunity, cold-chain dependence and retained virulence. CBPP can be diagnosed using post-mortem examination, identification of the agent using culture and PCR based methods as well as serological diagnostic methods, but the latter are generally not sensitive enough and there is also demand for an inexpensive, pen side field test.The research presented in this thesis was focused on using recombinantly expressed surface proteins from M. mycoides SC to characterize humoral immune responses to CBPP. Thereby candidate proteins to be used in development of serological diagnostic methods and possibly subunit vaccines could be identified. As a first step, five putative variable surface proteins of M. ...
Contagious Bovine Pleuropneumonia, formely under control, is once again a major threat to production across Africa, according to a report from the World Food and Agriculture Organisation.
Merck & Co., Inc., Kenilworth, NJ, USA is a global healthcare leader working to help the world be well. From developing new therapies that treat and prevent disease to helping people in need, we are committed to improving health and well-being around the world. The Merck Veterinary Manual was first published in 1955 as a service to the community. The legacy of this great resource continues as the Merck Veterinary Manual in the US and Canada and the MSD Manual outside of North America.. ...
The right lung (1) has a normal pink colour and has collapsed as the chest was opened. The left lung (2) has not collapsed; it is firm, discoloured and fleshy. It is coated with a yellow fibrin deposits, which are also present on the inside of the ribs. Often parts of the lungs adhere to the chest wall ...
Harrison, Freya and Muruli, Aneesha and Higgins, Steven and Diggle, Stephen P. (2014) Development of an ex vivo porcine lung model for studying growth, virulence, and signaling of Pseudomonas aeruginosa. Infection and Immunity, 82 (8). pp. 3312-3323. ISSN 1098-5522 ...
poractant alfa: RN given refers to cpd with unknown MF; isolated from porcine lungs; contains approx 99% phospholipids and 1% hydrophobic low MW (<15,000) proteins

Actinobacillus pleuropneumoniae infections in pigs: the role of virulence factors in pathogenesis and protection.Actinobacillus pleuropneumoniae infections in pigs: the role of virulence factors in pathogenesis and protection.

... Freddy ... "Actinobacillus Pleuropneumoniae Infections in Pigs: The Role of Virulence Factors in Pathogenesis and Protection." Veterinary ... "Actinobacillus Pleuropneumoniae Infections in Pigs: The Role of Virulence Factors in Pathogenesis and Protection." VETERINARY ... Haesebrouck F, Chiers K, Van Overbeke I, Ducatelle R. Actinobacillus pleuropneumoniae infections in pigs: the role of virulence ...
more infohttps://biblio.ugent.be/publication/176028

Putative biomarkers for evaluating antibiotic treatment: an experimental model of porcine Actinobacillus pleuropneumoniae...Putative biomarkers for evaluating antibiotic treatment: an experimental model of porcine Actinobacillus pleuropneumoniae...

Biomarkers of infection were screened for their possible role as evaluators of antibiotic treatment in an aerosol infection ... A similar Picture was seen for the biomarkers of infection. During the infection period, plasma C-reactive protein (CRP), ... an experimental model of porcine Actinobacillus pleuropneumoniae infection. Publication: Research - peer-review › Journal ... Classical swine fever virus infection modulates serum levels of INF-α, IL-8 and TNF-α in 6-month-old pigs. Publication: ...
more infohttp://orbit.dtu.dk/en/publications/putative-biomarkers-for-evaluating-antibiotic-treatment-an-experimental-model-of-porcine-actinobacillus-pleuropneumoniae-infection

Seroprevalence of Actinobacillus pleuropneumoniae serovars 2, 3 and 9 in slaughter pigs from Belgian fattening farms |...Seroprevalence of Actinobacillus pleuropneumoniae serovars 2, 3 and 9 in slaughter pigs from Belgian fattening farms |...

... selected fattening pig herds were studied to investigate the epidemiological characteristics of infections with Actinobacillus ... Seroprevalence of Actinobacillus pleuropneumoniae serovars 2, 3 and 9 in slaughter pigs from Belgian fattening farms ... Seroprevalence of Actinobacillus pleuropneumoniae serovars 2, 3 and 9 in slaughter pigs from Belgian fattening farms ...
more infohttp://veterinaryrecord.bmj.com/content/151/7/206

Caspase 1 involvement in human monocyte lysis induced by Actinobacillus actinomycetemcomitans leukotoxinCaspase 1 involvement in human monocyte lysis induced by Actinobacillus actinomycetemcomitans leukotoxin

2003 (Engelska)Ingår i: Infection and Immunity, ISSN 0019-9567, E-ISSN 1098-5522, Vol. 71, nr 8, s. 4448-4455Artikel i ... Periodontal infections with strains producing high levels of the leukotoxin are strongly associated with severe disease. ... Actinobacillus actinomycetemcomitans, an oral bacterium implicated in the etiology of periodontal diseases, produces a ... Caspase 1 involvement in human monocyte lysis induced by Actinobacillus actinomycetemcomitans leukotoxin. Kelk, Peyman Umeå ...
more infohttp://umu.diva-portal.org/smash/record.jsf?pid=diva2%3A146154&c=28&searchType=SIMPLE&language=sv&query=&af=%5B%5D&aq=%5B%5B%7B%22personId%22%3A%22authority-person%3A65531%22%7D%5D%5D&aq2=%5B%5B%5D%5D&aqe=%5B%5D&noOfRows=50&sortOrder=author_sort_asc&sortOrder2=title_sort_asc&onlyFullText=false&sf=all

Actinobacillus pleuropneumoniae - WikipediaActinobacillus pleuropneumoniae - Wikipedia

Overcrowding in pigpens, co-infections of other respiratory pathogens, and unusual stress all contribute to the spread of the ... Gillaspy, A. "Actinobacillus pleuropneumoniae". Laboratory for Molecular Biology and Cytometry Research-University of Oklahoma ... In 1995, A. pleuropneumoniae infections cost the US economy about $30 million. Such losses usually result from medication and ... "Actinobacillus pleuropneumoniae". Iowa State University College of Veterinary Medicine. Retrieved 20 April 2013. [permanent ...
more infohttps://en.wikipedia.org/wiki/Actinobacillus_pleuropneumoniae

ANTIMICROBIAL AND ANTIBIOFILM ACTIVITY OF CATHELICIDINS - Patent applicationANTIMICROBIAL AND ANTIBIOFILM ACTIVITY OF CATHELICIDINS - Patent application

0176] (12) Page M I, King E O. Infection due to Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus. The New ... Infection by these microbes is generally fended off by a variety of responses produced by the innate and adaptive immune system ... Overnight cultures were used for infection assays. Cell growth was monitored at O.D. 600 nm. The CFU/ml was determined with a ... 0178] (14) Blair T P, Seibel J, Jr., Oldfield E, Berg S W, Karney W, Baker W P. Endocarditis caused by Actinobacillus ...
more infohttp://www.patentsencyclopedia.com/app/20120149631

Estudio del comportamiento serológico de Actinobacillus pleuropneumoniae (App) en planteles porcinos comerciales de la zona...Estudio del comportamiento serológico de Actinobacillus pleuropneumoniae (App) en planteles porcinos comerciales de la zona...

... which seroconverted presumably due to a natural infection. Regarding maternal immunity duration it was found that around the ... MUNOZ, D; QUEZADA, M y RUIZ, A. Serological behaviour study of Actinobacillus pleuropneumoniae (App) in commercial swine herds ... Palabras clave : Actinobacillus pleuropneumoniae; seroprevalence; seroconversion. · resumen en Español · texto en Español · ...
more infohttp://www.scielo.cl/scielo.php?script=sci_abstract&pid=S0301-732X2008000200006&lng=es&nrm=iso&tlng=en

PROCESS FOR THE PRODUCTION OF THE ACTINOBACILLUS PLEUROPNEUMONIAE TOXIN     APXI EMPLOYING A CULTURE MEDIUM COMPRISING CALCIUM...PROCESS FOR THE PRODUCTION OF THE ACTINOBACILLUS PLEUROPNEUMONIAE TOXIN APXI EMPLOYING A CULTURE MEDIUM COMPRISING CALCIUM...

One of the ways to control Actinobacillus pleuropneumoniae (from now on also called "APP") infections is by vaccination ... 0004] It is commonly known how to produce the RTX-toxin ApxI by culturing Actinobacillus pleuropneumoniae in a culturing medium ... 1. A method to produce RTX-toxin ApxI comprising culturing Actinobacillus pleuropneumoniae bacteria in a culturing medium that ... The disease is primarily an infection of the respiratory tract having the clinical signs of high fever, severe respiratory ...
more infohttp://www.patentsencyclopedia.com/app/20110229935

Structures of the antigenic O-polysaccharides of lipopolysaccharides produced by Actinobacillus actinomycetemcomitans serotypes...Structures of the antigenic O-polysaccharides of lipopolysaccharides produced by Actinobacillus actinomycetemcomitans serotypes...

Structures of the antigenic O-polysaccharides of lipopolysaccharides produced by Actinobacillus actinomycetemcomitans serotypes ... INFECTIONS; LIPOPOLYSACCHARIDE; Lipopolysaccharides; LPS; Magnetic Resonance Spectroscopy; method; Methods; Methylation; Molar ... 13C-NMR; 1H-NMR; 6-deoxy-L-talose; Actinobacillus actinomycetemcomitans; Antigens; bacterial; Canada; chemical; chemistry; D- ... [email protected]: Structures of the antigenic O-polysaccharides of lipopolysaccharides produced by Actinobacillus actinomycetemcomitans ...
more infohttp://nparc.cisti-icist.nrc-cnrc.gc.ca/eng/view/object/?id=0bb3780b-5afa-483e-8aa9-624e7f4bca11

BacMapBacMap

It is transmitted short distances by droplet infection and only survives outside the pig for a few days.Actinobacillus ... The absence of apxICABD in strain JL03 confirms that it has the moderate toxicity specific to serotype 3.Actinobacillus ... Actinobacillus pleuropneumoniae, a Gram-negative, non-motile, facultatively anaerobic Pasteurellaceae, causes porcine ...
more infohttp://bacmap.wishartlab.com/organisms/721

American EnglishAmerican English

It is caused by Actinobacillus lignieresii, part of the normal bacterial flora of the upper digestive tract. ... It is caused by Actinobacillus lignieresii, part of the normal bacterial flora of the upper digestive tract. The bacteria ... Wooden tongue is a bacterial infection as a result of abrasions in the mouth. ... should not be brought back into the main herd until all symptoms have disappeared as they are a potential source of infection ...
more infohttp://www.farmhealthonline.com/US/disease-management/cattle-diseases/wooden-tongue/

Bulletin de lAcadémie Vétérinaire de France. SearchBulletin de l'Académie Vétérinaire de France. Search

Cette affection est due à Actinobacillus equuli mais on peut rencon trer Actinobacillus suis. Les formes classiques sont soit ... In one case 6 animals had acute gastro intestinal infection, 2 adults died, one during early phase of the disease, the other ... This affection is due to Actinobacillus equuli but sometimes to Actinobacillus suis. The clinical forms are abortion of the ... Les autres germes sont Escherichia coli, Klebsiella pneumoniae, Actinobacillus equuli, Salmonella typhimurium, Pseudomonas, Pro ...
more infohttp://documents.irevues.inist.fr/handle/2042/47354/discover?filtertype_0=dateIssued&filter_0=%5B1980+TO+1989%5D&filter_relational_operator_0=equals&filtertype=subject&filter_relational_operator=equals&filter=horse

Experimental actinobacillus seminis infection of rams : a scanning electron microscopy study of seminal vesiclesExperimental actinobacillus seminis infection of rams : a scanning electron microscopy study of seminal vesicles

... K-REx ... Experimental actinobacillus seminis infection of rams : a scanning electron microscopy study of seminal vesicles. Stockinger, ...
more infohttp://krex.k-state.edu/dspace/handle/2097/11886

Identification of QTL affecting resistance/susceptibility to acute Actinobacillus pleuropneumoniae infection in swine,...Identification of QTL affecting resistance/susceptibility to acute Actinobacillus pleuropneumoniae infection in swine,...

"Identification of QTL affecting resistance/susceptibility to acute Actinobacillus pleuropneumoniae infection in swine, ... Actinobacillus pleuropneumoniae infections in pigs: the role of virulence factors in pathogenesis and protection ... Identification of QTL affecting resistance/susceptibility to acute Actinobacillus pleuropneumoniae infection in swine. Reiner, ... Development of a DIVA subjunit vaccine against Actinobacillus pleuropneumoniae infection. Maas, A; Meens, J; Baltes, N; Hennig- ...
more infohttps://www.deepdyve.com/lp/springer_journal/identification-of-qtl-affecting-resistance-susceptibility-to-acute-x5tmFgbBh4

Actinobacillus seminis infection in sheep in the Republic of South Africa. I. Identification of the problemActinobacillus seminis infection in sheep in the Republic of South Africa. I. Identification of the problem

... Van Tonder, E.M.; ... Actinobacillus seminis infection in sheep in the Republic of South Africa. I. Identification of the problem. Login ... 1 vaccine was meticulously applied and the complete absence of Brucella ovis infection was established, of a total of 327 rams ... 25 out of 33 rams with subclinical infection and none out of 13 rams which showed no neutrophils in their semen. On 4 stud ...
more infohttps://repository.up.ac.za/handle/2263/53850

Effects of cations and osmotic protectants on cytolytic activity of Actinobacillus actinomycetemcomitans leukotoxin. |...Effects of cations and osmotic protectants on cytolytic activity of Actinobacillus actinomycetemcomitans leukotoxin. |...

Thank you for sharing this Infection and Immunity article.. NOTE: We request your email address only to inform the recipient ... Effects of cations and osmotic protectants on cytolytic activity of Actinobacillus actinomycetemcomitans leukotoxin.. M Iwase, ... Actinobacillus actinomycetemcomitans leukotoxin permeabilized the plasma membrane of HL-60 promyelocytic leukemia cells, ... Effects of cations and osmotic protectants on cytolytic activity of Actinobacillus actinomycetemcomitans leukotoxin. ...
more infohttps://iai.asm.org/content/58/6/1782

Killing of human myelomonocytic leukemia and lymphocytic cell lines by Actinobacillus actinomycetemcomitans leukotoxin. |...Killing of human myelomonocytic leukemia and lymphocytic cell lines by Actinobacillus actinomycetemcomitans leukotoxin. |...

Thank you for sharing this Infection and Immunity article.. NOTE: We request your email address only to inform the recipient ... Killing of human myelomonocytic leukemia and lymphocytic cell lines by Actinobacillus actinomycetemcomitans leukotoxin.. D L ... The purified leukotoxin of Actinobacillus actinomycetemcomitans kills human leukemic cell lines (e.g., HL-60, U937, and KG-1) ... Killing of human myelomonocytic leukemia and lymphocytic cell lines by Actinobacillus actinomycetemcomitans leukotoxin. ...
more infohttps://iai.asm.org/content/56/5/1162

Advanced Search Results - Public Health Image Library(PHIL)Advanced Search Results - Public Health Image Library(PHIL)

Categories: Actinobacillus Infections Image Types: Photo, Illustrations, Video, Color, Black&White, PublicDomain, ...
more infohttps://phil.cdc.gov/AdvancedSearchResults.aspx?Search=Actinobacillus+Infections&parentid=30891&catid=1899

Weight loss or illthriftWeight loss or illthrift

Cause - a bacterial infection (Actinobacillus lignieresi). This organism is normally present in the mouth of cattle. Under ... Cause - a fungal infection (Actinomyces bovis). This fungus is normally present in the mouth of healthy animals but it may ... Infection is usually acquired in calfhood but generally no clinical signs are seen until animals are at least four years old. ... Heavy infections cause a profuse watery scour which can lead to dehydration In yearlings and adult cattle a heavy infestation ...
more infohttps://www.dairyaustralia.com.au/farm/animal-management/animal-health/weight-loss-or-illthrift?section=enzootic_bovine_leaucosis

Code System ConceptCode System Concept

Actinobacillus infection (disorder) {16140007 , SNOMED-CT } Parent/Child (Relationship Type) Actinobacillary mastitis (disorder ... Actinobacillosis caused by Actinobacillus seminis (disorder) {47036002 , SNOMED-CT } Actinobacillosis caused by Actinobacillus ... Actinobacillosis caused by Actinobacillus lignieresi (disorder) {66701005 , SNOMED-CT } Actinobacillosis caused by ... 14390004 , SNOMED-CT } Actinobacillosis caused by Actinobacillus equuli (disorder) {371312007 , SNOMED-CT } ...
more infohttps://phinvads.cdc.gov/vads/ViewCodeSystemConcept.action?oid=2.16.840.1.113883.6.96&code=16140007

Paracoccus & sp: Causes & Reasons - Symptoma®Paracoccus & sp: Causes & Reasons - Symptoma®

Actinobacillus Infection Neisseria animaloris/zoodegmatis Ochrobactrum anthropi Oligella ureolytica Paracoccus yeei Pasteurella ... by biochemical and physiological tests is not routinely done, the occurrence of E. avium infections may be underestimated.[ncbi ...
more infohttps://www.symptoma.com/en/ddx/Paracoccus+sp.+R-28238

Multi-organ spreading of Actinobacillus pleuropneumoniae serovar 7 in weaned pigs during the first week after experimental...Multi-organ spreading of Actinobacillus pleuropneumoniae serovar 7 in weaned pigs during the first week after experimental...

... infection dose: 10.9 × 103 cfu/animal) were examined by culture during the first week after infection. In addition, tissue ... pleuropneumoniae serovar 7 strain AP 76 seems to occur more frequently during acute infection following effective lung ... Actinobacillus (A.) pleuropneumoniae is normally considered strictly adapted to the respiratory tract of swine. Despite this, ... Haesebrouck F, Chiers K, Van Overbeke I, Ducatelle R (1997) Actinobacillus pleuropneumoniae infections in pigs: the role of ...
more infohttps://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-018-0592-0

Code System ConceptCode System Concept

Actinobacillosis caused by Actinobacillus suis Current Synonym true false 54085010 Actinobacillosis due to Actinobacillus suis ... Actinobacillus infection (disorder) {16140007 , SNOMED-CT } Other Relationships No other relationships present. ... Actinobacillosis caused by Actinobacillus suis (disorder). Code System Preferred Concept Name. Actinobacillosis caused by ...
more infohttps://phinvads.cdc.gov/vads/ViewCodeSystemConcept.action?oid=2.16.840.1.113883.6.96&code=32389002

Kato K[au] - PubMed - NCBIKato K[au] - PubMed - NCBI

Septicemic Actinobacillus suis infection in a neonatal piglet with multifocal necrotic glossitis. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed?cmd=search&term=Kato+K%5Bau%5D&dispmax=50
  • Several studies have shown a greater prevalence of HLA-B27 antigen in WD patients (26% versus 8% in European and American populations, respectively) ( 16 , 22 ), but no causal association between HLA-B27 presence and infection susceptibility has been demonstrated. (asm.org)
  • The World Health Organization (WHO) reports that licensed vaccines are currently available for twenty-five different preventable infections . (wikipedia.org)
  • Through an in-depth examination of the mechanisms used by the microbial adversaries to evade or otherwise subvert the host's immune response, we may be able to devise more effective live vaccines and develop novel therapeutic strategies for the treatment of persistent infections, as well as to discover valuable immunomodulatory agents. (asmscience.org)
  • A clinical palpation and semen smear examination of 647 rams submitted to the Regional Veterinary Laboratory during 1967 revealed that 42 (6,5%) of these animals had clinical epididymitis or orchitis, 6 (0,9%) showed other types of genital lesions and 98 (15,1%) suffered from subclinical genital infection. (up.ac.za)
  • A. seminis and A. seminis-like organisms were isolated from semen specimens of 18 out of 35 rams with clinical epididymitis or orchitis, 25 out of 33 rams with subclinical infection and none out of 13 rams which showed no neutrophils in their semen. (up.ac.za)
  • On 4 stud farms where Elberg Rev. 1 vaccine was meticulously applied and the complete absence of Brucella ovis infection was established, of a total of 327 rams examined, 10 (3,6%) were found to be clinically and 72 (22,0%) subclinically affected. (up.ac.za)
  • A. seminis was isolated from 5 out of 6 of these rams with clinical lesions and 10 out of 15 of those which showed evidence of subclinical infection. (up.ac.za)