Fatigue: The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.Actinin: A protein factor that regulates the length of R-actin. It is chemically similar, but immunochemically distinguishable from actin.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Systems Integration: The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Proteome: The protein complement of an organism coded for by its genome.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Actinin: A protein factor that regulates the length of R-actin. It is chemically similar, but immunochemically distinguishable from actin.Creatine Kinase, BB Form: A form of creatine kinase found in the BRAIN.Microfilament Proteins: Monomeric subunits of primarily globular ACTIN and found in the cytoplasmic matrix of almost all cells. They are often associated with microtubules and may play a role in cytoskeletal function and/or mediate movement of the cell or the organelles within the cell.Sarcomeres: The repeating contractile units of the MYOFIBRIL, delimited by Z bands along its length.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Connectin: A giant elastic protein of molecular mass ranging from 2,993 kDa (cardiac), 3,300 kDa (psoas), to 3,700 kDa (soleus) having a kinase domain. The amino- terminal is involved in a Z line binding, and the carboxy-terminal region is bound to the myosin filament with an overlap between the counter-connectin filaments at the M line.Myofibrils: The long cylindrical contractile organelles of STRIATED MUSCLE cells composed of ACTIN FILAMENTS; MYOSIN filaments; and other proteins organized in arrays of repeating units called SARCOMERES .Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Actinin: A protein factor that regulates the length of R-actin. It is chemically similar, but immunochemically distinguishable from actin.Microfilament Proteins: Monomeric subunits of primarily globular ACTIN and found in the cytoplasmic matrix of almost all cells. They are often associated with microtubules and may play a role in cytoskeletal function and/or mediate movement of the cell or the organelles within the cell.Actins: Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.Filamins: A family of crosslinking filament proteins encoded by distinct FLN genes. Filamins are involved in cell adhesion, spreading, and migration, acting as scaffolds for over 90 binding partners including channels, receptors, intracellular signaling molecules and transcription factors. Due to the range of molecular interactions, mutations in FLN genes result in anomalies with moderate to lethal consequences.Cross-Linking Reagents: Reagents with two reactive groups, usually at opposite ends of the molecule, that are capable of reacting with and thereby forming bridges between side chains of amino acids in proteins; the locations of naturally reactive areas within proteins can thereby be identified; may also be used for other macromolecules, like glycoproteins, nucleic acids, or other.Contractile Proteins: Proteins which participate in contractile processes. They include MUSCLE PROTEINS as well as those found in other cells and tissues. In the latter, these proteins participate in localized contractile events in the cytoplasm, in motile activity, and in cell aggregation phenomena.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Actin Cytoskeleton: Fibers composed of MICROFILAMENT PROTEINS, which are predominately ACTIN. They are the smallest of the cytoskeletal filaments.Microscopy, Immunoelectron: Microscopy in which the samples are first stained immunocytochemically and then examined using an electron microscope. Immunoelectron microscopy is used extensively in diagnostic virology as part of very sensitive immunoassays.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Actinin: A protein factor that regulates the length of R-actin. It is chemically similar, but immunochemically distinguishable from actin.Creatine Kinase, BB Form: A form of creatine kinase found in the BRAIN.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Microfilament Proteins: Monomeric subunits of primarily globular ACTIN and found in the cytoplasmic matrix of almost all cells. They are often associated with microtubules and may play a role in cytoskeletal function and/or mediate movement of the cell or the organelles within the cell.Reagent Kits, Diagnostic: Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.GermanySTAT3 Transcription Factor: A signal transducer and activator of transcription that mediates cellular responses to INTERLEUKIN-6 family members. STAT3 is constitutively activated in a variety of TUMORS and is a major downstream transducer for the CYTOKINE RECEPTOR GP130.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Reindeer: A genus of deer, Rangifer, that inhabits the northern parts of Europe, Asia, and America. Caribou is the North American name; reindeer, the European. They are often domesticated and used, especially in Lapland, for drawing sleds and as a source of food. Rangifer is the only genus of the deer family in which both sexes are antlered. Most caribou inhabit arctic tundra and surrounding arboreal coniferous forests and most have seasonal shifts in migration. They are hunted extensively for their meat, skin, antlers, and other parts. (From Webster, 3d ed; Walker's Mammals of the World, 5th ed, p1397)Alphaherpesvirinae: A subfamily of HERPESVIRIDAE characterized by a short replication cycle. The genera include: SIMPLEXVIRUS; VARICELLOVIRUS; MAREK'S DISEASE-LIKE VIRUSES; and ILTOVIRUS.Actinin: A protein factor that regulates the length of R-actin. It is chemically similar, but immunochemically distinguishable from actin.Spectrin: A high molecular weight (220-250 kDa) water-soluble protein which can be extracted from erythrocyte ghosts in low ionic strength buffers. The protein contains no lipids or carbohydrates, is the predominant species of peripheral erythrocyte membrane proteins, and exists as a fibrous coating on the inner, cytoplasmic surface of the membrane.Creatine Kinase, BB Form: A form of creatine kinase found in the BRAIN.Microfilament Proteins: Monomeric subunits of primarily globular ACTIN and found in the cytoplasmic matrix of almost all cells. They are often associated with microtubules and may play a role in cytoskeletal function and/or mediate movement of the cell or the organelles within the cell.Dystrophin: A muscle protein localized in surface membranes which is the product of the Duchenne/Becker muscular dystrophy gene. Individuals with Duchenne muscular dystrophy usually lack dystrophin completely while those with Becker muscular dystrophy have dystrophin of an altered size. It shares features with other cytoskeletal proteins such as SPECTRIN and alpha-actinin but the precise function of dystrophin is not clear. One possible role might be to preserve the integrity and alignment of the plasma membrane to the myofibrils during muscle contraction and relaxation. MW 400 kDa.Utrophin: An autosomally-encoded 376-kDa cytoskeletal protein that is similar in structure and function to DYSTROPHIN. It is a ubiquitously-expressed protein that plays a role in anchoring the CYTOSKELETON to the PLASMA MEMBRANE.Dystrophin-Associated Proteins: A group of proteins that associate with DYSTROPHIN at the CELL MEMBRANE to form the DYSTROPHIN-ASSOCIATED PROTEIN COMPLEX.Ankyrins: A family of membrane-associated proteins responsible for the attachment of the cytoskeleton. Erythrocyte-related isoforms of ankyrin attach the SPECTRIN cytoskeleton to a transmembrane protein (ANION EXCHANGE PROTEIN 1, ERYTHROCYTE) in the erythrocyte plasma membrane. Brain-related isoforms of ankyrin also exist.Muscular Dystrophy, Duchenne: An X-linked recessive muscle disease caused by an inability to synthesize DYSTROPHIN, which is involved with maintaining the integrity of the sarcolemma. Muscle fibers undergo a process that features degeneration and regeneration. Clinical manifestations include proximal weakness in the first few years of life, pseudohypertrophy, cardiomyopathy (see MYOCARDIAL DISEASES), and an increased incidence of impaired mentation. Becker muscular dystrophy is a closely related condition featuring a later onset of disease (usually adolescence) and a slowly progressive course. (Adams et al., Principles of Neurology, 6th ed, p1415)Pan troglodytes: The common chimpanzee, a species of the genus Pan, family HOMINIDAE. It lives in Africa, primarily in the tropical rainforests. There are a number of recognized subspecies.ArchivesBiological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Directories as Topic: Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)Publishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.Actinin: A protein factor that regulates the length of R-actin. It is chemically similar, but immunochemically distinguishable from actin.Serial Publications: Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)Publications: Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)Social Sciences: Disciplines concerned with the interrelationships of individuals in a social environment including social organizations and institutions. Includes Sociology and Anthropology.Actinin: A protein factor that regulates the length of R-actin. It is chemically similar, but immunochemically distinguishable from actin.EF Hand Motifs: Calcium-binding motifs composed of two helices (E and F) joined by a loop. Calcium is bound by the loop region. These motifs are found in many proteins that are regulated by calcium.Microfilament Proteins: Monomeric subunits of primarily globular ACTIN and found in the cytoplasmic matrix of almost all cells. They are often associated with microtubules and may play a role in cytoskeletal function and/or mediate movement of the cell or the organelles within the cell.Dictyostelium: A genus of protozoa, formerly also considered a fungus. Its natural habitat is decaying forest leaves, where it feeds on bacteria. D. discoideum is the best-known species and is widely used in biomedical research.Actins: Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.Filamins: A family of crosslinking filament proteins encoded by distinct FLN genes. Filamins are involved in cell adhesion, spreading, and migration, acting as scaffolds for over 90 binding partners including channels, receptors, intracellular signaling molecules and transcription factors. Due to the range of molecular interactions, mutations in FLN genes result in anomalies with moderate to lethal consequences.Cross-Linking Reagents: Reagents with two reactive groups, usually at opposite ends of the molecule, that are capable of reacting with and thereby forming bridges between side chains of amino acids in proteins; the locations of naturally reactive areas within proteins can thereby be identified; may also be used for other macromolecules, like glycoproteins, nucleic acids, or other.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Transglutaminases: Transglutaminases catalyze cross-linking of proteins at a GLUTAMINE in one chain with LYSINE in another chain. They include keratinocyte transglutaminase (TGM1 or TGK), tissue transglutaminase (TGM2 or TGC), plasma transglutaminase involved with coagulation (FACTOR XIII and FACTOR XIIIa), hair follicle transglutaminase, and prostate transglutaminase. Although structures differ, they share an active site (YGQCW) and strict CALCIUM dependence.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
(1/917) Gamma-Actinin, a new regulatory protein from rabbit skeletal muscle. I. Purification and characterization.

A new regulatory protein which we have designated as gamma-actinin has been isolated from native thin filaments of rabbit skeletal muscle. Depolymerized native thin filaments were fractionated by salting out with ammonium sulfate, and the precipitates obtained at 40--60% ammonium sulfate saturation were further subjected to DEAE-Sephadex and Sephadex G-200 column chromatography. The purified gamma-actinin was shown to have a chain weight of 35,000 daltons and had a strong inhibitory action on the polymerization of G-actin. The results of amino acid analysis indicated a unique amino acid composition of gamma-actinin as compared with other structural proteins of muscle. Non-polar and neutral amino acid residues were abundant. One cysteine residue was contained per one molecule of gamma-actinin and played a critical role in the maintenance of the inhibitory activity. Pelleting of gamma-actinin with F-actin showed that gamma-actinin binds to F-action.  (+info)

(2/917) Polypyrimidine tract binding protein functions as a repressor to regulate alternative splicing of alpha-actinin mutally exclusive exons.

The smooth muscle (SM) and nonmuscle (NM) isoforms of alpha-actinin are produced by mutually exclusive splicing of an upstream NM exon and a downstream SM-specific exon. A rat alpha-actinin genomic clone encompassing the mutually exclusive exons was isolated and sequenced. The SM exon was found to utilize two branch points located 382 and 386 nucleotides (nt) upstream of the 3' splice site, while the NM exon used a single branch point 191 nt upstream. Mutually exclusive splicing arises from the proximity of the SM branch points to the NM 5' splice site, and this steric repression could be relieved in part by the insertion of spacer elements. In addition, the SM exon is repressed in non-SM cells and extracts. In vitro splicing of spacer-containing transcripts could be activated by (i) truncation of the transcript between the SM polypyrimidine tract and exon, (ii) addition of competitor RNAs containing the 3' end of the actinin intron or regulatory sequences from alpha-tropomyosin (TM), and (iii) depletion of the splicing extract by using biotinylated alpha-TM RNAs. A number of lines of evidence point to polypyrimidine tract binding protein (PTB) as the trans-acting factor responsible for repression. PTB was the only nuclear protein observed to cross-link to the actinin RNA, and the ability of various competitor RNAs to activate splicing correlated with their ability to bind PTB. Furthermore, repression of alpha-actinin splicing in the nuclear extracts depleted of PTB by using biotinylated RNA could be specifically restored by the addition of recombinant PTB. Thus, alpha-actinin mutually exclusive splicing is enforced by the unusual location of the SM branch point, while constitutive repression of the SM exon is conferred by regulatory elements between the branch point and 3' splice site and by PTB.  (+info)

(3/917) Tropomodulin assembles early in myofibrillogenesis in chick skeletal muscle: evidence that thin filaments rearrange to form striated myofibrils.

Actin filament lengths in muscle and nonmuscle cells are believed to depend on the regulated activity of capping proteins at both the fast growing (barbed) and slow growing (pointed) filament ends. In striated muscle, the pointed end capping protein, tropomodulin, has been shown to maintain the lengths of thin filaments in mature myofibrils. To determine whether tropomodulin might also be involved in thin filament assembly, we investigated the assembly of tropomodulin into myofibrils during differentiation of primary cultures of chick skeletal muscle cells. Our results show that tropomodulin is expressed early in differentiation and is associated with the earliest premyofibrils which contain overlapping and misaligned actin filaments. In addition, tropomodulin can be found in actin filament bundles at the distal tips of growing myotubes, where sarcomeric alpha-actinin is not always detected, suggesting that tropomodulin caps actin filament pointed ends even before the filaments are cross-linked into Z bodies by alpha-actinin. Tropomodulin staining exhibits an irregular punctate pattern along the length of premyofibrils that demonstrate a smooth phalloidin staining pattern for F-actin. Strikingly, the tropomodulin dots often appear to be located between the closely spaced, dot-like Z bodies that are stained for (&agr;)-actinin. Thus, in the earliest premyofibrils, the pointed ends of the thin filaments are clustered and partially aligned with respect to the Z bodies (the location of the barbed filament ends). At later stages of differentiation, the tropomodulin dots become aligned into regular periodic striations concurrently with the appearance of striated phalloidin staining for F-actin and alignment of Z bodies into Z lines. Tropomodulin, together with the barbed end capping protein, CapZ, may function from the earliest stages of myofibrillogenesis to restrict the lengths of newly assembled thin filaments by capping their ends; thus, transitions from nonstriated to striated myofibrils in skeletal muscle are likely due principally to filament rearrangements rather than to filament polymerization or depolymerization. Rearrangements of actin filaments capped at their pointed and barbed ends may be a general mechanism by which cells restructure their actin cytoskeletal networks during cell growth and differentiation.  (+info)

(4/917) An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment.

The LIM domain protein zyxin is a component of adherens type junctions, stress fibers, and highly dynamic membrane areas and appears to be involved in microfilament organization. Chicken zyxin and its human counterpart display less than 60% sequence identity, raising concern about their functional identity. Here, we demonstrate that human zyxin, like the avian protein, specifically interacts with alpha-actinin. Furthermore, we map the interaction site to a motif of approximately 22 amino acids, present in the N-terminal domain of human zyxin. This motif is both necessary and sufficient for alpha-actinin binding, whereas a downstream region, which is related in sequence, appears to be dispensable. A synthetic peptide comprising human zyxin residues 21-42 specifically binds to alpha-actinin in solid phase binding assays. In contrast to full-length zyxin, constructs lacking this motif do not interact with alpha-actinin in blot overlays and fail to recruit alpha-actinin in living cells. When zyxin lacking the alpha-actinin binding site is expressed as a fusion protein with green fluorescent protein, association of the recombinant protein with stress fibers is abolished, and targeting to focal adhesions is grossly impaired. Our results suggest a crucial role for the alpha-actinin-zyxin interaction in subcellular zyxin localization and microfilament organization.  (+info)

(5/917) Viscoelastic properties of f-actin, microtubules, f-actin/alpha-actinin, and f-actin/hexokinase determined in microliter volumes with a novel nondestructive method.

A nondestructive method to determine viscoelastic properties of gels and fluids involves an oscillating glass fiber serving as a sensor for the viscosity of the surrounding fluid. Extremely small displacements (typically 1-100 nm) are caused by the glass rod oscillating at its resonance frequency. These displacements are analyzed using a phase-sensitive acoustic microscope. Alterations of the elastic modulus of a fluid or gel change the propagation speed of a longitudinal acoustic wave. The system allows to study quantities as small as 10 microliters with temporal resolution >1 Hz. For 2-100 microM f-actin gels a final viscosity of 1.3-9.4 mPa s and a final elastic modulus of 2.229-2.254 GPa (corresponding to 1493-1501 m/s sound velocity) have been determined. For 10- to 100-microM microtubule gels (native, without stabilization by taxol), a final viscosity of 1.5-124 mPa s and a final elastic modulus of 2.288-2. 547 GPa (approximately 1513-1596 m/s) have been determined. During polymerization the sound velocity in low-concentration actin solutions increased up to +1.3 m/s (approximately 1.69 kPa) and decreased up to -7 m/s (approximately 49 kPa) at high actin concentrations. On polymerization of tubulin a concentration-dependent decrease of sound velocity was observed, too (+48 to -12 m/s approximately 2.3-0.1 MPa, for 10- to 100-microM tubulin). This decrease was interpreted by a nematic phase transition of the actin filaments and microtubules with increasing concentration. 2 mM ATP (when compared to 0.2 mM ATP) increased polymerization rate, final viscosity and elastic modulus of f-actin (17 microM). The actin-binding glycolytic enzyme hexokinase also accelerated the polymerization rate and final viscosity but elastic modulus (2.26 GPa) was less than for f-actin polymerized in presence of 0.2 mM ATP (2.28 GPa).  (+info)

(6/917) Cardiac microvascular endothelial cells express alpha-smooth muscle actin and show low NOS III activity.

We established a culture system of porcine coronary microvascular endothelial cells (MVEC) with high cellular yield and purity >98%. Endothelial origin was confirmed by immunostaining, immunoblotting and fluorescence-activated cell sorter (FACS) analysis using low-density lipoprotein uptake, CD31, von Willebrand factor, and the lectin Dolichos biflorus agglutinin. MVEC were positive for alpha-smooth muscle actin in culture and in myocardium, as confirmed by FACS. Of the primary MVEC, approximately 30% expressed nitric oxide synthase (NOS) III in numbers decreasing from the first passage (6 +/- 1%) to the second passage (4 +/- 1%; P < 0.001 vs. primary isolates), whereas approximately 100% of aortic endothelial cells (AEC) expressed NOS III. In AEC, NOS III activity (pmol citrulline. mg protein-1. min-1) was 80 +/- 10 and was nearly abolished in the absence of calcium (5 +/- 1, P < 0.001). In primary MVEC, however, NOS III activity in the presence and absence of calcium was 20 +/- 4 and 25 +/- 5, respectively. We conclude that cardiac MVEC, in contrast to AEC, contain alpha-smooth muscle actin, show low-grade NOS III activity, and provide a suitable in vitro system for the study of endothelial pathophysiology.  (+info)

(7/917) Myotilin, a novel sarcomeric protein with two Ig-like domains, is encoded by a candidate gene for limb-girdle muscular dystrophy.

The striated muscle sarcomeres are highly organized structures composed of actin (thin) and myosin (thick) filaments that slide past each other during contraction. The integrity of sarcomeres is controlled by a set of structural proteins, among which are titin, a giant molecule that contains several immunoglobulin (Ig)-like domains and associates with thin and thick filaments, and [alpha]-actinin, an actin cross-linking protein. Mutations in several sarcomeric and sarcolemmal proteins have been shown to result in muscular dystrophy and cardiomyopathy. On the other hand, the disease genes underlying several disease forms remain to be identified. Here we describe a novel 57 kDa cytoskeletal protein, myotilin. Its N-terminal sequence is unique, but the C-terminal half contains two Ig-like domains homologous to titin. Myotilin is expressed in skeletal and cardiac muscle, it co-localizes with [alpha]-actinin in the sarcomeric I--bands and directly interacts with [alpha]-actinin. The human myotilin gene maps to chromosome 5q31 between markers AFM350yB1 and D5S500. The locus of a dominantly inherited limb-girdle muscular dystrophy (LGMD1A) resides in an overlapping narrow segment, and a new type of distal myopathy with vocal cord and pharyngeal weakness (VCPMD) has been mapped to the same locus. The muscle specificity and apparent role as a sarcomeric structural protein raise the possibility that defects in the myotilin gene may cause muscular dystrophy.  (+info)

(8/917) Postmortem proteolysis and calpain/calpastatin activity in callipyge and normal lamb biceps femoris during extended postmortem storage.

The present experiment was conducted to determine whether calpastatin inhibits only the rate, or both the rate and extent, of calpain-induced postmortem proteolysis. Biceps femoris from normal (n = 6) and callipyge (n = 6) lamb was stored for 56 d at 4 degrees C. Calpastatin activity was higher (P < .05) in the callipyge muscle at 0 and 14 d postmortem, but not at 56 d postmortem. The activity of mu-calpain did not differ between normal and callipyge biceps femoris at 0 and 56 d postmortem (P > .05), but was higher at 14 d postmortem in the callipyge muscle (P < 0.05). The activity of m-calpain was higher in the callipyge muscle (P < 0.05). Western blot analyses of titin, nebulin, dystrophin, myosin heavy chain, vinculin, alpha-actinin, desmin, and troponin-T indicated that postmortem proteolysis was less extensive in callipyge than in normal biceps femoris at all postmortem times. The results of this experiment indicate that calpastatin inhibits both the rate and extent of postmortem proteolysis.  (+info)

*  ACTC1
Cardiac alpha actin is a filamentous protein extending from a complex mesh with cardiac alpha-actinin (ACTN2) at Z-lines ...
*  Actinin
... is a microfilament protein. α-Actinin is necessary for the attachment of actin filaments to the Z-lines in skeletal ... The non-sarcomeric α-actinins, encoded by ACTN1 and ACTN4, are widely expressed. ACTN2 expression is found in both cardiac and ... Both ends of the rod-shaped α-actinin dimer contain actin-binding domains. Mutations in ACTN4 can cause the kidney disease ... ISBN 978-0-08-092427-4. Actinin at the US National Library of Medicine Medical Subject Headings (MeSH). ...
*  Actinin alpha 3
Alpha-actinin-3, also known as alpha-actinin skeletal muscle isoform 3 or F-actin cross-linking protein, is a protein that in ... ACTN3 has been shown to interact with Actinin, alpha 2. Actinin GRCh38: Ensembl release 89: ENSG00000248746 - Ensembl, May 2017 ... Alpha-actinin is an actin-binding protein with multiple roles in different cell types. This gene expression is limited to ... Actin filaments are stabilized by actin binding proteins known as actinins of which there are two main types, type 2 and type 3 ...
*  Actinin alpha 4
Alpha-actinin-4 is a protein that in humans is encoded by the ACTN4 gene. Alpha actinins belong to the spectrin gene ... Vallenius T, Luukko K, Mäkelä TP (2000). "CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4 ... "CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4". J. Biol. Chem. UNITED STATES. 275 (15): ... Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal ...
*  Actinin alpha 1
Alpha-actinin-1 is a protein that in humans is encoded by the ACTN1 gene. Alpha actinins belong to the spectrin gene ... 1999). "An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment". ... "CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4". J. Biol. Chem. 275 (15): 11100-5. doi: ... "An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment". J. Biol ...
*  Actinin alpha 2
Alpha-actinin 2 is a protein which in humans is encoded by the ACTN2 gene. This gene encodes an alpha-actinin isoform that is ... The high-resolution crystal structure of human alpha-actinin 2 at 3.5 Å was recently resolved. Alpha actinins belong to the ... Alpha-actinin 2 is a 103.8 kDa protein composed of 894 amino acids. Each molecule is rod-shaped (35 nm in length) and it ... Alpha-actinin 2 has been shown to interact with KCNA5, DLG1, DISC1, MYOZ1, GRIN2B, ADAM12, ACTN3, MYPN, PDLIM3, PKN, MYOT, TTN ...
*  Protein kinase N1
... has been shown to interact with: AKAP9, Actinin, alpha 1, CCDC85B, NEFL, NEUROD2 Phosphoinositide-dependent ... "Interaction of PKN with alpha-actinin". J. Biol. Chem. 272 (8): 4740-6. doi:10.1074/jbc.272.8.4740. PMID 9030526. Matsuzawa K, ... "Pathological shear stress stimulates the tyrosine phosphorylation of alpha-actinin associated with the glycoprotein Ib-IX ...
*  Nebulette
... alpha-actinin. Xin, and XIRP2. Nebulette was identified in 1995 by Moncman and Wang using primary cultures of chicken embryonic ...
*  Zyxin
"An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment". J. Biol ... "An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment". J. Biol ... Li B, Trueb B (2001). "Analysis of the alpha-actinin/zyxin interaction". J. Biol. Chem. 276 (36): 33328-35. doi:10.1074/jbc. ... Li B, Trueb B (September 2001). "Analysis of the alpha-actinin/zyxin interaction". J. Biol. Chem. 276 (36): 33328-35. doi: ...
*  CAPZB
CapZ interacts with α-actinin, nebulette, nebulin, HSC70. at the Z-disc. CAPZB is a member of the F-actin capping protein ... Papa I, Astier C, Kwiatek O, Raynaud F, Bonnal C, Lebart MC, Roustan C, Benyamin Y (February 1999). "Alpha actinin-CapZ, an ...
*  F-actin capping protein
Maruyama K, Kurokawa H, Oosawa M, Shimaoka S, Yamamoto H, Ito M, Maruyama K (May 1990). "Beta-actinin is equivalent to Cap Z ...
*  Collagen, type XVII, alpha 1
... has been shown to interact with Keratin 18, Actinin alpha 4, Dystonin, Actinin, alpha 1, CTNND1 ... Gonzalez, A M; Otey C; Edlund M; Jones J C (December 2001). "Interactions of a hemidesmosome component and actinin family ...
*  Keith Burridge
They decided to compile their work on α-actinin and showed that α-actinin is distributed periodically along stress fibers. They ... Since these regions would several years later be named focal adhesions, α-actinin was the first protein found to be ... While developing a procedure to purify α-actinin from smooth muscle, Burridge co-purified another protein, vinculin, ... Lazarides E, Burridge K; Burridge (November 1975). "Alpha-actinin: immunofluorescent localization of a muscle structural ...
*  Actin
Liu Y, Belkina NV, Shaw S (2009). "HIV infection of T cells: actin-in and actin-out". Science Signaling. 2 (66): pe23. doi: ... Actin filaments are linked to α-actinin and to the membrane through vinculin. The head domain of vinculin associates to E- ... For example, if the α-actinin or gelation factor gene has been removed in Dictyostelium individuals do not show an anomalous ... In contractile bundles, the actin-bundling protein alpha-actinin separates each thin filament by ~35 nm. This increase in ...
*  Polycystin cation channel family
TRPP3 is abundant in mouse brain where it associates with alpha-actinin-2. Alpha-actinin attaches TRPP3 to the cytoskeleton and ... Alpha-actinin is an actin-bundling protein known to regulate several types of ion channels. Planer lipid bilayer ... The TRPP3-alpha-actinin association was documented by co-immunoprecipitation using native cells and tissues, yeast two-hybrid, ... "Direct binding of alpha-actinin enhances TRPP3 channel activity". Journal of Neurochemistry. 103 (6): 2391-2400. doi:10.1111/j. ...
*  LPP (gene)
Li B, Zhuang L, Reinhard M, Trueb B (2003). "The lipoma preferred partner LPP interacts with alpha-actinin". J. Cell Sci. 116 ( ...
*  CAMK2B
... has been shown to interact with Actinin alpha 4. GRCh38: Ensembl release 89: ENSG00000058404 - Ensembl, May 2017 GRCm38 ... actinin". J. Neurosci. 21 (2): 423-33. PMID 11160423. Liao GY, Wagner DA, Hsu MH, Leonard JP (2001). "Evidence for direct ... actinin". J. Neurosci. 21 (2): 423-33. PMID 11160423. Yamamoto H (2002). "[Molecular mechanisms of the intracellular ...
*  CDK5R2
... has been shown to interact with Actinin, alpha 1. GRCh38: Ensembl release 89: ENSG00000171450 - Ensembl, May 2017 GRCm38 ... 5 activators p35 and p39 interact with the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II and alpha-actinin-1 in ... 5 activators p35 and p39 interact with the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II and alpha-actinin-1 in ...
*  Hydroxyacid oxidase (glycolate oxidase) 1
Lan S, Wang H, Jiang H, Mao H, Liu X, Zhang X, Hu Y, Xiang L, Yuan Z (2003). "Direct interaction between α-actinin and ...
*  TRIM3
The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin ... 2005). "CART: an Hrs/actinin-4/BERP/myosin V protein complex required for efficient receptor recycling". Mol. Biol. Cell. 16 (5 ... 2000). "BERP, a novel ring finger protein, binds to alpha-actinin-4". Biochem. Biophys. Res. Commun. 267 (3): 906-11. doi: ... TRIM3 has been shown to interact with Actinin alpha 4. GRCh38: Ensembl release 89: ENSG00000110171 - Ensembl, May 2017 GRCm38: ...
*  TTC4
2003). "Direct interaction between alpha-actinin and hepatitis C virus NS5B". FEBS Lett. 554 (3): 289-94. doi:10.1016/S0014- ...
*  LDB2
... with alpha-actinin 2". J Cell Biochem. 78 (4): 558-65. doi:10.1002/1097-4644(20000915)78:4. 3.0.CO;2-I. PMID 10861853. "Entrez ...
*  Acid-sensing ion channel
Namely, α-Actinin results in heightened pH sensitivity and desensitization recovery. They can also increase current flow ...
*  GRIN2B
Wyszynski M, Lin J, Rao A, Nigh E, Beggs AH, Craig AM, Sheng M (January 1997). "Competitive binding of alpha-actinin and ... Actinin, alpha 2, DLG2, DLG3, DLG4, EXOC4, LIN7B, and RICS. NMDA receptor Glutamate receptor GRCh38: Ensembl release 89: ...
*  Sporadic late onset nemaline myopathy
Immunostains for myotilin and α-actinin all but clinch the diagnosis. However, nemaline rods may still be visible post-mortem ...
Recombinant Human Sarcomeric Alpha Actinin protein (ab92234) References  Recombinant Human Sarcomeric Alpha Actinin protein (ab92234) References
References for Abcam's Recombinant Human Sarcomeric Alpha Actinin protein (ab92234). Please let us know if you have used this ...
more infohttp://www.abcam.com/recombinant-human-sarcomeric-alpha-actinin-protein-ab92234-references.html
Navigieren Sie durch unsere alpha Actinin ELISA Kits  Navigieren Sie durch unsere alpha Actinin ELISA Kits
alpha Actinin ELISA Kits für viele Reaktivitäten. Huhn, Rind (Kuh), Hund und weitere. alpha Actinin ELISA Kits vergleichen und ... alpha-actinin-2 , alpha-actinin-3 , alpha-actinin , alpha-actinin-4 , non-muscle alpha-actinin 4 ... alpha Actinin (ACTN) ELISA Kits. Bezeichnung:. alpha-Actinin Skeletal Muscle ELISA Kits (ACTN). Auf www.antikoerper-online.de ... the interaction between GNE (zeige GNE ELISA Kits) and alpha-actinin 1 (zeige ACTN1 ELISA Kits) and alpha-actinin 2 (zeige ...
more infohttp://www.antikoerper-online.de/cell-cell-junction-organization-pathway-74/alpha-actinin-elisa-kit-6016/
Actinin alpha (pan) - AP06786PU-N | acris-antibodies.com  Actinin alpha (pan) - AP06786PU-N | acris-antibodies.com
The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. ... There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to ... Product Description for Actinin alpha (pan). Rabbit anti Human, Mouse, Rat Actinin alpha (pan).. Presentation: Aff - Purified. ... Recommended Secondary Antibodies for Actinin alpha (pan) (9 products). Catalog No.. Host. Clone/Iso.. Pres.. React.. ...
more infohttps://www.acris-antibodies.com/antibodies/primary-antibodies/actinin-alpha-pan-ap06786pu-n.htm
Gudrun Wahlstr m: From actin monomers to bundles: The roles of twinfilin and α-actinin in <i>Drosophila melanogaster</i...  Gudrun Wahlstr m: From actin monomers to bundles: The roles of twinfilin and α-actinin in <i>Drosophila melanogaster</i...
The population of α-actinin protein present in non-muscle cells of the ActnΔ233 mutant is referred to as FC-α-actinin (Follicle ... α-Actinin has been implicated in several diverse cell biological processes. In Drosophila, the only function for α-actinin yet ... Therefore, α-actinin expression, regulation and function in this tissue were further analysed. Examination of the α-actinin ... At late oogenesis, the main body follicle cells express both non-muscle α-actinin and FC-α-actinin, while the dorsal anterior ...
more infohttp://ethesis.helsinki.fi/julkaisut/bio/bioja/vk/wahlstrom/abstract.html
Anti-alpha Actinin 4 抗体 (ab59468) | アブカム  Anti-alpha Actinin 4 抗体 (ab59468) | アブカム
"ウサギ・ポリクローナル抗体 ab59468 交差種: Ms,Hu 適用: WB,IHC-P,ICC/IF…alpha Actinin 4抗体一覧…画像、プロトコール、文献などWeb上の情報 ... Anti-alpha Actinin 4 antibody. alpha Actinin 4 一次抗体 製品一覧. ... Anti-alpha Actinin 4 antibody (ab59468) at 1/1000 dilution + Whole cell lysates prepared from mouse embryonic fibroblasts at 10 ... Immunocytochemistry/ Immunofluorescence - alpha Actinin 4 antibody (ab59468)This image
more infohttp://www.abcam.co.jp/alpha-actinin-4-antibody-ab59468.html
alpha Actinin 4 (phospho S159)抗体| Abcam中国  alpha Actinin 4 (phospho S159)抗体| Abcam中国
... alpha Actinin 4抗体经WB,ELISA验证,可与人,小鼠,大鼠,Monkey样本 ... 购买alpha Actinin 4兔多克隆抗体(ab117411), ... Anti-alpha Actinin 4 (phospho S159)抗体. 参阅全部 alpha Actinin 4 一抗 ... Belongs to the alpha-actinin family.. Contains 1 actin-binding domain.. Contains 2 CH (calponin-homology) domains.. Contains 2 ... Synthetic phospho-peptide, corresponding to a region within amino acids 140-190 of Human alpha Actinin
more infohttp://www.abcam.cn/alpha-actinin-4-phospho-s159-antibody-ab117411.html
Anti-Sarcomeric Alpha Actinin抗体[EP2529Y] (ab68167)参考文献  Anti-Sarcomeric Alpha Actinin抗体[EP2529Y] (ab68167)参考文献
Hodges JL et al. a-Actinin-2 mediates spine morphology and assembly of the post-synaptic density in hippocampal neurons. PLoS ...
more infohttp://www.abcam.cn/sarcomeric-alpha-actinin-antibody-ep2529y-ab68167-references.html
Disuse-induced Preferential Loss of the Giant Protein Titin Depresses Muscle Performance via Abnormal Sarcomeric Organization |...  Disuse-induced Preferential Loss of the Giant Protein Titin Depresses Muscle Performance via Abnormal Sarcomeric Organization |...
C) Analyses of major sarcomere proteins, i.e., M-protein (left gel; 8%), MyBP-C, α-actinin, actin (middle gel, 8%), troponin T ...
more infohttp://jgp.rupress.org/content/131/1/33
Alpha-actinin (L7UZ85) | InterPro | EMBL-EBI  Alpha-actinin (L7UZ85) | InterPro | EMBL-EBI
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
more infohttp://www.ebi.ac.uk/interpro/protein/L7UZ85
Alpha-actinin-4 (O43707) | InterPro | EMBL-EBI  Alpha-actinin-4 (O43707) | InterPro | EMBL-EBI
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
more infohttp://www.ebi.ac.uk/interpro/protein/O43707
Actn1 actinin, alpha 1 [Mus musculus (house mouse)] - Gene - NCBI  Actn1 actinin, alpha 1 [Mus musculus (house mouse)] - Gene - NCBI
alpha-actinin-1. Names. F-actin cross-linking protein. alpha actinin 1a. alpha-actinin cytoskeletal isoform. non-muscle alpha- ... Alpha-actinin interaction with syndecan-4 has a role in stabilising cell-matrix adhesion. Title: Alpha-actinin interactions ... Actn1 actinin, alpha 1 [Mus musculus] Actn1 actinin, alpha 1 [Mus musculus]. Gene ID:109711 ... actinin, alpha 1provided by MGI. Primary source. MGI:MGI:2137706 See related. Ensembl:ENSMUSG00000015143 Gene type. protein ...
more infohttps://www.ncbi.nlm.nih.gov/gene/?term=109711
Actinin, alpha 2 - wikidoc  Actinin, alpha 2 - wikidoc
1997). "Interaction of PKN with alpha-actinin". J. Biol. Chem. 272 (8): 4740-6. PMID 9030526.. CS1 maint: Explicit use of et al ... 2001). "FATZ, a filamin-, actinin-, and telethonin-binding protein of the Z-disc of skeletal muscle". J. Biol. Chem. 275 (52): ... 2000). "Interaction of hCLIM1, an enigma family protein, with alpha-actinin 2". J. Cell. Biochem. 78 (4): 558-65. PMID 10861853 ... Actinin, alpha 2, also known as ACTN2, is a human gene.[1] ... Alpha actinin is an actin-binding protein with multiple roles ...
more infohttp://wikidoc.org/index.php?title=Actinin,_alpha_2&printable=yes
Anti-Sarcomeric Alpha Actinin antibody (ab72592) References  Anti-Sarcomeric Alpha Actinin antibody (ab72592) References
Anti-Sarcomeric Alpha Actinin antibody (ab72592) has been cited in 20 publications. References for Human, Mouse, Rat in IF, IHC ...
more infohttps://www.abcam.com/sarcomeric-alpha-actinin-antibody-ab72592-references.html
alpha-actinin-2 ELISA Kits | Biocompare.com  alpha-actinin-2 ELISA Kits | Biocompare.com
Compare alpha-actinin-2 ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and ... alpha-actinin-2 ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established antibody-based tool for ... Your search returned 110 alpha-actinin-2 ELISA ELISA Kit across 14 suppliers. ...
more infohttps://www.biocompare.com/pfu/110627/soids/2-49898/Assay_Kit/ELISA_alpha-actinin-2
Alpha Actinin 2 Proteins: Novus Biologicals  Alpha Actinin 2 Proteins: Novus Biologicals
Browse our Alpha Actinin 2 Protein catalog backed by our Guarantee+. ... Alternate Names for Alpha Actinin 2 Proteins. Alpha Actinin 2 protein, ACTN2 protein, actinin, alpha 2 protein, Alpha-actinin ... Alpha Actinin 2 Proteins. We offer Alpha Actinin 2 Peptides and Alpha Actinin 2 Proteins for use in common research ... Our Alpha Actinin 2 Peptides and Alpha Actinin 2 Proteins can be used in a variety of model species: Human. Use the list below ...
more infohttps://www.novusbio.com/proteins/alpha-actinin-2?related_pathways=Muscle%20Cell%20Differentiation
Alpha Actinin 2 Proteins: Novus Biologicals  Alpha Actinin 2 Proteins: Novus Biologicals
Browse our Alpha Actinin 2 Protein catalog backed by our Guarantee+. ... Alternate Names for Alpha Actinin 2 Proteins. Alpha Actinin 2 protein, ACTN2 protein, actinin, alpha 2 protein, Alpha-actinin ... Alpha Actinin 2 Proteins. We offer Alpha Actinin 2 Peptides and Alpha Actinin 2 Proteins for use in common research ... Our Alpha Actinin 2 Peptides and Alpha Actinin 2 Proteins can be used in a variety of model species: Human. Use the list below ...
more infohttps://www.novusbio.com/proteins/alpha-actinin-2
Anti-Alpha-Actinin-4 Antibody Products | Biocompare.com  Anti-Alpha-Actinin-4 Antibody Products | Biocompare.com
Compare Anti-Alpha-Actinin-4 Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, ...
more infohttps://www.biocompare.com/pfu/110447/soids/337280/Antibodies/Alpha-Actinin-4
ACTN3 - Alpha-actinin-3 - Bos taurus (Bovine) - ACTN3 gene & protein  ACTN3 - Alpha-actinin-3 - Bos taurus (Bovine) - ACTN3 gene & protein
PS00019. ACTININ_1. 1 hit. PS00020. ACTININ_2. 1 hit. PS50021. CH. 2 hits. PS50222. EF_HAND_2. 2 hits. ... PS00019. ACTININ_1. 1 hit. PS00020. ACTININ_2. 1 hit. PS50021. CH. 2 hits. PS50222. EF_HAND_2. 2 hits. ... IPR001589. Actinin_actin-bd_CS. IPR036872. Calponin-like_dom_sf. IPR001715. CH-domain. IPR011992. EF-hand-dom_pair. IPR014837. ... IPR001589. Actinin_actin-bd_CS. IPR036872. Calponin-like_dom_sf. IPR001715. CH-domain. IPR011992. EF-hand-dom_pair. IPR014837. ...
more infohttp://www.uniprot.org/uniprot/Q0III9
Alpha-actinin | definition of alpha-actinin by Medical dictionary  Alpha-actinin | definition of alpha-actinin by Medical dictionary
... alpha-actinin explanation free. What is alpha-actinin? Meaning of alpha-actinin medical term. What does alpha-actinin mean? ... Looking for online definition of alpha-actinin in the Medical Dictionary? ... alpha-actinin. α-ac·tin·in. (ak-tin'in), An F-actin binding protein in vertebrate cells that cross-links actin filaments into ... Actin-binding protein alpha-actinin 4 (ACTN4) is a transcriptional co-activator of RelA/p65 sub-unit of NF-kB.. The Short ...
more infohttp://medical-dictionary.thefreedictionary.com/alpha-actinin
ACTN4 - Actinin alpha 4 - Macaca mulatta (Rhesus macaque) - ACTN4 gene & protein  ACTN4 - Actinin alpha 4 - Macaca mulatta (Rhesus macaque) - ACTN4 gene & protein
PS00019 ACTININ_1, 1 hit. PS00020 ACTININ_2, 1 hit. PS50021 CH, 2 hits. PS00018 EF_HAND_1, 1 hit. PS50222 EF_HAND_2, 2 ... IPR001589 Actinin_actin-bd_CS. IPR029637 ACTN4. IPR001715 CH-domain. IPR036872 CH_dom_sf. IPR011992 EF-hand-dom_pair. IPR014837 ... Actinin alpha 4Imported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a ... tr,F7HU82,F7HU82_MACMU Actinin alpha 4 OS=Macaca mulatta OX=9544 GN=ACTN4 PE=2 SV=1 ...
more infohttps://www.uniprot.org/uniprot/F7HU82
  • More broadly though, this structure presents a picture of the intimate interactions between the subunits in the actinin dimer, providing a framework to better understand the molecular details of actin cross-linking and its regulation in all actinins and perhaps in spectrins too. (springer.com)
  • α-actinins have been highly conserved throughout evolution and are largely collinear proteins that share three conserved functional domains. (miltenyibiotec.com)
  • a-Actinin-3 deficiency alters muscle adaptation in response to denervation and immobilization. (edu.au)
  • a-Actinin-3 deficiency alters muscle adaptation in response to denervation and immobilization. (edu.au)
  • ?-actinin-3 , a special protein expressed only in fast twitch fibres and commonly associated with speed, power and strength in athletes. (fitlion.com)