Actinin
Gamma-Actinin, a new regulatory protein from rabbit skeletal muscle. I. Purification and characterization. (1/917)
A new regulatory protein which we have designated as gamma-actinin has been isolated from native thin filaments of rabbit skeletal muscle. Depolymerized native thin filaments were fractionated by salting out with ammonium sulfate, and the precipitates obtained at 40--60% ammonium sulfate saturation were further subjected to DEAE-Sephadex and Sephadex G-200 column chromatography. The purified gamma-actinin was shown to have a chain weight of 35,000 daltons and had a strong inhibitory action on the polymerization of G-actin. The results of amino acid analysis indicated a unique amino acid composition of gamma-actinin as compared with other structural proteins of muscle. Non-polar and neutral amino acid residues were abundant. One cysteine residue was contained per one molecule of gamma-actinin and played a critical role in the maintenance of the inhibitory activity. Pelleting of gamma-actinin with F-actin showed that gamma-actinin binds to F-action. (+info)Polypyrimidine tract binding protein functions as a repressor to regulate alternative splicing of alpha-actinin mutally exclusive exons. (2/917)
The smooth muscle (SM) and nonmuscle (NM) isoforms of alpha-actinin are produced by mutually exclusive splicing of an upstream NM exon and a downstream SM-specific exon. A rat alpha-actinin genomic clone encompassing the mutually exclusive exons was isolated and sequenced. The SM exon was found to utilize two branch points located 382 and 386 nucleotides (nt) upstream of the 3' splice site, while the NM exon used a single branch point 191 nt upstream. Mutually exclusive splicing arises from the proximity of the SM branch points to the NM 5' splice site, and this steric repression could be relieved in part by the insertion of spacer elements. In addition, the SM exon is repressed in non-SM cells and extracts. In vitro splicing of spacer-containing transcripts could be activated by (i) truncation of the transcript between the SM polypyrimidine tract and exon, (ii) addition of competitor RNAs containing the 3' end of the actinin intron or regulatory sequences from alpha-tropomyosin (TM), and (iii) depletion of the splicing extract by using biotinylated alpha-TM RNAs. A number of lines of evidence point to polypyrimidine tract binding protein (PTB) as the trans-acting factor responsible for repression. PTB was the only nuclear protein observed to cross-link to the actinin RNA, and the ability of various competitor RNAs to activate splicing correlated with their ability to bind PTB. Furthermore, repression of alpha-actinin splicing in the nuclear extracts depleted of PTB by using biotinylated RNA could be specifically restored by the addition of recombinant PTB. Thus, alpha-actinin mutually exclusive splicing is enforced by the unusual location of the SM branch point, while constitutive repression of the SM exon is conferred by regulatory elements between the branch point and 3' splice site and by PTB. (+info)Tropomodulin assembles early in myofibrillogenesis in chick skeletal muscle: evidence that thin filaments rearrange to form striated myofibrils. (3/917)
Actin filament lengths in muscle and nonmuscle cells are believed to depend on the regulated activity of capping proteins at both the fast growing (barbed) and slow growing (pointed) filament ends. In striated muscle, the pointed end capping protein, tropomodulin, has been shown to maintain the lengths of thin filaments in mature myofibrils. To determine whether tropomodulin might also be involved in thin filament assembly, we investigated the assembly of tropomodulin into myofibrils during differentiation of primary cultures of chick skeletal muscle cells. Our results show that tropomodulin is expressed early in differentiation and is associated with the earliest premyofibrils which contain overlapping and misaligned actin filaments. In addition, tropomodulin can be found in actin filament bundles at the distal tips of growing myotubes, where sarcomeric alpha-actinin is not always detected, suggesting that tropomodulin caps actin filament pointed ends even before the filaments are cross-linked into Z bodies by alpha-actinin. Tropomodulin staining exhibits an irregular punctate pattern along the length of premyofibrils that demonstrate a smooth phalloidin staining pattern for F-actin. Strikingly, the tropomodulin dots often appear to be located between the closely spaced, dot-like Z bodies that are stained for (&agr;)-actinin. Thus, in the earliest premyofibrils, the pointed ends of the thin filaments are clustered and partially aligned with respect to the Z bodies (the location of the barbed filament ends). At later stages of differentiation, the tropomodulin dots become aligned into regular periodic striations concurrently with the appearance of striated phalloidin staining for F-actin and alignment of Z bodies into Z lines. Tropomodulin, together with the barbed end capping protein, CapZ, may function from the earliest stages of myofibrillogenesis to restrict the lengths of newly assembled thin filaments by capping their ends; thus, transitions from nonstriated to striated myofibrils in skeletal muscle are likely due principally to filament rearrangements rather than to filament polymerization or depolymerization. Rearrangements of actin filaments capped at their pointed and barbed ends may be a general mechanism by which cells restructure their actin cytoskeletal networks during cell growth and differentiation. (+info)An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment. (4/917)
The LIM domain protein zyxin is a component of adherens type junctions, stress fibers, and highly dynamic membrane areas and appears to be involved in microfilament organization. Chicken zyxin and its human counterpart display less than 60% sequence identity, raising concern about their functional identity. Here, we demonstrate that human zyxin, like the avian protein, specifically interacts with alpha-actinin. Furthermore, we map the interaction site to a motif of approximately 22 amino acids, present in the N-terminal domain of human zyxin. This motif is both necessary and sufficient for alpha-actinin binding, whereas a downstream region, which is related in sequence, appears to be dispensable. A synthetic peptide comprising human zyxin residues 21-42 specifically binds to alpha-actinin in solid phase binding assays. In contrast to full-length zyxin, constructs lacking this motif do not interact with alpha-actinin in blot overlays and fail to recruit alpha-actinin in living cells. When zyxin lacking the alpha-actinin binding site is expressed as a fusion protein with green fluorescent protein, association of the recombinant protein with stress fibers is abolished, and targeting to focal adhesions is grossly impaired. Our results suggest a crucial role for the alpha-actinin-zyxin interaction in subcellular zyxin localization and microfilament organization. (+info)Viscoelastic properties of f-actin, microtubules, f-actin/alpha-actinin, and f-actin/hexokinase determined in microliter volumes with a novel nondestructive method. (5/917)
A nondestructive method to determine viscoelastic properties of gels and fluids involves an oscillating glass fiber serving as a sensor for the viscosity of the surrounding fluid. Extremely small displacements (typically 1-100 nm) are caused by the glass rod oscillating at its resonance frequency. These displacements are analyzed using a phase-sensitive acoustic microscope. Alterations of the elastic modulus of a fluid or gel change the propagation speed of a longitudinal acoustic wave. The system allows to study quantities as small as 10 microliters with temporal resolution >1 Hz. For 2-100 microM f-actin gels a final viscosity of 1.3-9.4 mPa s and a final elastic modulus of 2.229-2.254 GPa (corresponding to 1493-1501 m/s sound velocity) have been determined. For 10- to 100-microM microtubule gels (native, without stabilization by taxol), a final viscosity of 1.5-124 mPa s and a final elastic modulus of 2.288-2. 547 GPa (approximately 1513-1596 m/s) have been determined. During polymerization the sound velocity in low-concentration actin solutions increased up to +1.3 m/s (approximately 1.69 kPa) and decreased up to -7 m/s (approximately 49 kPa) at high actin concentrations. On polymerization of tubulin a concentration-dependent decrease of sound velocity was observed, too (+48 to -12 m/s approximately 2.3-0.1 MPa, for 10- to 100-microM tubulin). This decrease was interpreted by a nematic phase transition of the actin filaments and microtubules with increasing concentration. 2 mM ATP (when compared to 0.2 mM ATP) increased polymerization rate, final viscosity and elastic modulus of f-actin (17 microM). The actin-binding glycolytic enzyme hexokinase also accelerated the polymerization rate and final viscosity but elastic modulus (2.26 GPa) was less than for f-actin polymerized in presence of 0.2 mM ATP (2.28 GPa). (+info)Cardiac microvascular endothelial cells express alpha-smooth muscle actin and show low NOS III activity. (6/917)
We established a culture system of porcine coronary microvascular endothelial cells (MVEC) with high cellular yield and purity >98%. Endothelial origin was confirmed by immunostaining, immunoblotting and fluorescence-activated cell sorter (FACS) analysis using low-density lipoprotein uptake, CD31, von Willebrand factor, and the lectin Dolichos biflorus agglutinin. MVEC were positive for alpha-smooth muscle actin in culture and in myocardium, as confirmed by FACS. Of the primary MVEC, approximately 30% expressed nitric oxide synthase (NOS) III in numbers decreasing from the first passage (6 +/- 1%) to the second passage (4 +/- 1%; P < 0.001 vs. primary isolates), whereas approximately 100% of aortic endothelial cells (AEC) expressed NOS III. In AEC, NOS III activity (pmol citrulline. mg protein-1. min-1) was 80 +/- 10 and was nearly abolished in the absence of calcium (5 +/- 1, P < 0.001). In primary MVEC, however, NOS III activity in the presence and absence of calcium was 20 +/- 4 and 25 +/- 5, respectively. We conclude that cardiac MVEC, in contrast to AEC, contain alpha-smooth muscle actin, show low-grade NOS III activity, and provide a suitable in vitro system for the study of endothelial pathophysiology. (+info)Myotilin, a novel sarcomeric protein with two Ig-like domains, is encoded by a candidate gene for limb-girdle muscular dystrophy. (7/917)
The striated muscle sarcomeres are highly organized structures composed of actin (thin) and myosin (thick) filaments that slide past each other during contraction. The integrity of sarcomeres is controlled by a set of structural proteins, among which are titin, a giant molecule that contains several immunoglobulin (Ig)-like domains and associates with thin and thick filaments, and [alpha]-actinin, an actin cross-linking protein. Mutations in several sarcomeric and sarcolemmal proteins have been shown to result in muscular dystrophy and cardiomyopathy. On the other hand, the disease genes underlying several disease forms remain to be identified. Here we describe a novel 57 kDa cytoskeletal protein, myotilin. Its N-terminal sequence is unique, but the C-terminal half contains two Ig-like domains homologous to titin. Myotilin is expressed in skeletal and cardiac muscle, it co-localizes with [alpha]-actinin in the sarcomeric I--bands and directly interacts with [alpha]-actinin. The human myotilin gene maps to chromosome 5q31 between markers AFM350yB1 and D5S500. The locus of a dominantly inherited limb-girdle muscular dystrophy (LGMD1A) resides in an overlapping narrow segment, and a new type of distal myopathy with vocal cord and pharyngeal weakness (VCPMD) has been mapped to the same locus. The muscle specificity and apparent role as a sarcomeric structural protein raise the possibility that defects in the myotilin gene may cause muscular dystrophy. (+info)Postmortem proteolysis and calpain/calpastatin activity in callipyge and normal lamb biceps femoris during extended postmortem storage. (8/917)
The present experiment was conducted to determine whether calpastatin inhibits only the rate, or both the rate and extent, of calpain-induced postmortem proteolysis. Biceps femoris from normal (n = 6) and callipyge (n = 6) lamb was stored for 56 d at 4 degrees C. Calpastatin activity was higher (P < .05) in the callipyge muscle at 0 and 14 d postmortem, but not at 56 d postmortem. The activity of mu-calpain did not differ between normal and callipyge biceps femoris at 0 and 56 d postmortem (P > .05), but was higher at 14 d postmortem in the callipyge muscle (P < 0.05). The activity of m-calpain was higher in the callipyge muscle (P < 0.05). Western blot analyses of titin, nebulin, dystrophin, myosin heavy chain, vinculin, alpha-actinin, desmin, and troponin-T indicated that postmortem proteolysis was less extensive in callipyge than in normal biceps femoris at all postmortem times. The results of this experiment indicate that calpastatin inhibits both the rate and extent of postmortem proteolysis. (+info)
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alpha Actinin 4 (phospho S159)抗体| Abcam中国
Gudrun Wahlstr m: From actin monomers to bundles: The roles of twinfilin and α-actinin in <i>Drosophila melanogaster</i...
Recombinant Human Sarcomeric Alpha Actinin protein (ab92234) References
Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) References
Anti-Sarcomeric Alpha Actinin抗体[EP2529Y] (ab68167)参考文献
Alpha Actinin 4 Antibody (4D10) [DyLight 488] (H00000081-M01G): Novus Biologicals
Anti-alpha Actinin 4 抗体 (ab59468) | アブカム
New N-RAP-binding partners α-actinin, filamin and Krp1 detected by yeast two-hybrid screening: implications for myofibril...
Actinin alpha (pan) - AP06786PU-N | acris-antibodies.com
Diane Taylor - taylor | taylor
Anti-Sarcomeric Alpha Actinin 抗体 [EP2529Y] (ab68167)
The role of the actin-binding domain in the intracellular localization by Raymond William Washington
DISEASES - ACTN4
G9a, a putative histone methyl-transferase in Drosophila interacts with Tungus, a protein associated with α-Actinin
KAKEN - Research Projects | Basic fibroblast growth factor induces downregulation of alpha smooth muscle actinin dermal...
Novel α-Actinin 2 Variant Associated With Familial Hypertrophic Cardiomyopathy and Juvenile Atrial ArrhythmiasCLINICAL...
TRIP Database - ơ-actinin / TRP channels
The effect of alpha-actinin-3 deficiency on muscle aging | Garvan Institute of Medical Research
alpha-actinin-2 in rat striatum: localization and interaction with NMDA glutamate receptor subunits.
Anti-Alpha-Actinin Antibody (Monoclonal, EA-53) - Citomed
The Ca(2+)-binding domains in non-muscle type alpha-actinin: biochemical and genetic analysis. | JCB
Sandwalk: Which Animal Phylum Has the Most Species?
Human ACTN1 protein | EUPROTEIN
MCQ Physiology Muscles Ques. and Answers | Physiology Plus
Two Cell Adhesion Molecules, Nectin and Cadherin, Interact through Their Cytoplasmic Domain-Associated Proteins | JCB
Increased Neointima Formation in Cysteine-Rich Protein 2-Deficient Mice in Response to Vascular Injury | Circulation Research
Lmo7 - LMO7a - Rattus norvegicus (Rat) - Lmo7 gene & protein
IJMS | Free Full-Text | Large-scale Models Reveal the Two-component Mechanics of Striated Muscle | Notes
Smooth Muscle Cell Origin and Its Relation to Heterogeneity in Development and Disease | Arteriosclerosis, Thrombosis, and...
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ACTN3 - Alpha-actinin-3 - Bos taurus (Bovine) - ACTN3 gene & protein
Loss of CH-ILKBP impairs the membrane translocation of | Open-i
GENES FOR SPEED | SCQ
Biological Mechanism of FSGS-1 - Martin Pollak
ACTN1-AS1 Gene - GeneCards | ACTN1-AS1 RNA Gene
Corrigendum to α-Actinin TvACTN3 of Trichomonas vaginalis Is an RNA-Binding Protein That Could Participate in Its...
The relationship between stress fiber-like structures and nascent myofibrils in cultured cardiac myocytes. | Journal of Cell...
Actin Binding Protein Spin-Down Assay Biochem Kit (skeletal actin) - Cytoskeleton, Inc.
C3a receptor blockade protects podocytes from injury in diabetic nephropathy<...
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Localization of Islet-1-Positive Cells in the Healthy and Infarcted Adult Murine Heart | Circulation Research
test were utilized - mTOR Inhibitors in Parkinsons Disease
Identification, characterization, and synthesis of peptide epitopes an | ITT
US20080177327A1 - Central rod connector and T-rod - Google Patents
PARVB - PrimePCR Assay and Template | Life Science | Bio-Rad
Alpha-actinin (L7UZ85) | InterPro | EMBL-EBI
IQGAP2 IQ motif containing GTPase activating protein 2 [Homo sapiens (human)] - Gene - NCBI
Protein kinase N1
... has been shown to interact with: AKAP9, Actinin, alpha 1, CCDC85B, NEFL, NEUROD2 Phosphoinositide-dependent ... "Interaction of PKN with alpha-actinin". J. Biol. Chem. 272 (8): 4740-6. doi:10.1074/jbc.272.8.4740. PMID 9030526. Matsuzawa K, ... "Pathological shear stress stimulates the tyrosine phosphorylation of alpha-actinin associated with the glycoprotein Ib-IX ...
Nebulette
... alpha-actinin. Xin, and XIRP2. Nebulette was identified in 1995 by Moncman and Wang using primary cultures of chicken embryonic ...
Zyxin
"An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment". J. Biol ... "An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment". J. Biol ... Li B, Trueb B (2001). "Analysis of the alpha-actinin/zyxin interaction". J. Biol. Chem. 276 (36): 33328-35. doi:10.1074/jbc. ... Li B, Trueb B (September 2001). "Analysis of the alpha-actinin/zyxin interaction". J. Biol. Chem. 276 (36): 33328-35. doi: ...
CAPZB
CapZ interacts with α-actinin, nebulette, nebulin, HSC70. at the Z-disc. CAPZB is a member of the F-actin capping protein ... Papa I, Astier C, Kwiatek O, Raynaud F, Bonnal C, Lebart MC, Roustan C, Benyamin Y (February 1999). "Alpha actinin-CapZ, an ...
F-actin capping protein
Maruyama K, Kurokawa H, Oosawa M, Shimaoka S, Yamamoto H, Ito M, Maruyama K (May 1990). "Beta-actinin is equivalent to Cap Z ...
Collagen, type XVII, alpha 1
... has been shown to interact with Keratin 18, Actinin alpha 4, Dystonin, Actinin, alpha 1, CTNND1 ... Gonzalez, A M; Otey C; Edlund M; Jones J C (December 2001). "Interactions of a hemidesmosome component and actinin family ...
Keith Burridge
They decided to compile their work on α-actinin and showed that α-actinin is distributed periodically along stress fibers. They ... Since these regions would several years later be named focal adhesions, α-actinin was the first protein found to be ... While developing a procedure to purify α-actinin from smooth muscle, Burridge co-purified another protein, vinculin, ... Lazarides E, Burridge K; Burridge (November 1975). "Alpha-actinin: immunofluorescent localization of a muscle structural ...
Actin
Liu Y, Belkina NV, Shaw S (2009). "HIV infection of T cells: actin-in and actin-out". Science Signaling. 2 (66): pe23. doi: ... For example, if the α-actinin or gelation factor gene has been removed in Dictyostelium individuals do not show an anomalous ... The contractile ring is composed of actin, myosin, anillin, and α-actinin. In the fission yeast Schizosaccharomyces pombe, ... Fujiwara K, Porter ME, Pollard TD (Oct 1978). "Alpha-actinin localization in the cleavage furrow during cytokinesis". The ...
Polycystin cation channel family
TRPP3 is abundant in mouse brain where it associates with alpha-actinin-2. Alpha-actinin attaches TRPP3 to the cytoskeleton and ... Li Q, Dai XQ, Shen PY, Wu Y, Long W, Chen CX, Hussain Z, Wang S, Chen XZ (December 2007). "Direct binding of alpha-actinin ... Li Q, Dai XQ, Shen PY, Wu Y, Long W, Chen CX, Hussain Z, Wang S, Chen XZ (December 2007). "Direct binding of alpha-actinin ... Alpha-actinin is an actin-bundling protein known to regulate several types of ion channels. Planer lipid bilayer ...
LPP (gene)
Li B, Zhuang L, Reinhard M, Trueb B (2003). "The lipoma preferred partner LPP interacts with alpha-actinin". J. Cell Sci. 116 ( ...
CAMK2B
... has been shown to interact with Actinin alpha 4. GRCh38: Ensembl release 89: ENSG00000058404 - Ensembl, May 2017 GRCm38 ... actinin". J. Neurosci. 21 (2): 423-33. doi:10.1523/JNEUROSCI.21-02-00423.2001. PMC 6763799. PMID 11160423. Liao GY, Wagner DA, ... actinin". J. Neurosci. 21 (2): 423-33. doi:10.1523/JNEUROSCI.21-02-00423.2001. PMC 6763799. PMID 11160423. Yamamoto H (2002 ...
CDK5R2
... has been shown to interact with Actinin, alpha 1. GRCh38: Ensembl release 89: ENSG00000171450 - Ensembl, May 2017 GRCm38 ... 5 activators p35 and p39 interact with the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II and alpha-actinin-1 in ... 5 activators p35 and p39 interact with the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II and alpha-actinin-1 in ...
Hydroxyacid oxidase (glycolate oxidase) 1
Lan S, Wang H, Jiang H, Mao H, Liu X, Zhang X, Hu Y, Xiang L, Yuan Z (2003). "Direct interaction between α-actinin and ...
TRIM3
Yan Q, Sun W, Kujala P, Lotfi Y, Vida TA, Bean AJ (May 2005). "CART: an Hrs/actinin-4/BERP/myosin V protein complex required ... The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin ... TRIM3 has been shown to interact with Actinin alpha 4. TRIM3 binds to and ubiquitinates Estrogen receptor alpha (ERa) leading ... binds to alpha-actinin-4". Biochemical and Biophysical Research Communications. 267 (3): 906-911. doi:10.1006/bbrc.1999.2045. ...
TTC4
2003). "Direct interaction between alpha-actinin and hepatitis C virus NS5B". FEBS Lett. 554 (3): 289-94. doi:10.1016/S0014- ...
LDB2
... with alpha-actinin 2". J Cell Biochem. 78 (4): 558-65. doi:10.1002/1097-4644(20000915)78:4. 3.0.CO;2-I. PMID 10861853. S2CID ...
Acid-sensing ion channel
Namely, α-Actinin results in heightened pH sensitivity and desensitization recovery. They can also increase current flow ...
GRIN2B
Wyszynski M, Lin J, Rao A, Nigh E, Beggs AH, Craig AM, Sheng M (January 1997). "Competitive binding of alpha-actinin and ... Actinin, alpha 2, DLG2, DLG3, DLG4, EXOC4, LIN7B, and RICS. NMDA receptor Glutamate receptor GRCh38: Ensembl release 89: ...
Sporadic late-onset nemaline myopathy
Immunostains for myotilin and α-actinin all but clinch the diagnosis. However, nemaline rods may still be visible post-mortem ...
Adherens junction
... "alpha-Actinin localization in the junctional complex of intestinal epithelial cells". J Cell Biol. 80 (1): 203-210. doi:10.1083 ...
Palladin
2005). "Involvement of palladin and alpha-actinin in targeting of the Abl/Arg kinase adaptor ArgBP2 to the actin cytoskeleton ... 2004). "Molecular analysis of the interaction between palladin and alpha-actinin". FEBS Lett. 566 (1-3): 30-4. doi:10.1016/j. ...
PDLIM1
Vallenius T, Luukko K, Mäkelä TP (2000). "CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4 ... PDLIM1 has been shown to interact with: Actinin alpha 4, Actinin, alpha 1, Estrogen receptor alpha, and RNF12. GRCh38: Ensembl ... "CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4". J. Biol. Chem. 275 (15): 11100-5. doi: ... with alpha-actinin 2". J Cell Biochem. 78 (4): 558-65. doi:10.1002/1097-4644(20000915)78:4. 3.0.CO;2-I. PMID 10861853. S2CID ...
Stress fiber
The antiparallel arrangement of actin filaments within stress fibers is reinforced by α-actinin, an actin filament crosslinking ... Lazarides, Elias; Burridge, Keith (November 1975). "α-Actinin: Immunofluorescent localization of a muscle structural protein in ... such as α-actinin, to form a highly regulated actomyosin structure within non-muscle cells. Stress fibers have been shown to ... fibers in motile and non-motile cells are similar in that they both contain actin filaments which are cross-linked by α-actinin ...
C17orf98
There are no RNA binding motifs or actinin type actin binding motifs. There are no N-myristoylation pattern or prenylation ...
CSRP1
Harper BD, Beckerle MC, Pomiès P (2001). "Fine mapping of the alpha-actinin binding site within cysteine-rich protein". Biochem ...
Dendrin
Within the brain, dendrin interacts with α-actinin in postsynaptic dendritic spines. Together MAGI/S-SCAM, α-actinin, and ... Along with two other proteins, MAGI/S-SCAM and α-actinin, dendrin is linked to synaptic plasticity and memory formation in the ... The protein trio of MAGI/S-SCAM, α-actinin, and dendrin helps to connect postsynaptic density (PSD) to the cytoskeleton of the ...
Spectrin repeat
These include spectrin, alpha-actinin, dystrophin and more recently the plakin family. The spectrin repeat forms a three-helix ...
PARVB
Olski TM, Noegel AA, Korenbaum E (Feb 2001). "Parvin, a 42 kDa focal adhesion protein, related to the alpha-actinin superfamily ... 2004). "Affixin interacts with alpha-actinin and mediates integrin signaling for reorganization of F-actin induced by initial ...
PARVA
Olski TM, Noegel AA, Korenbaum E (Feb 2001). "Parvin, a 42 kDa focal adhesion protein, related to the alpha-actinin superfamily ...
Integrin beta 1
Otey CA, Vasquez GB, Burridge K, Erickson BW (Oct 1993). "Mapping of the alpha-actinin binding site within the beta 1 integrin ... Otey CA, Pavalko FM, Burridge K (Aug 1990). "An interaction between alpha-actinin and the beta 1 integrin subunit in vitro". ...
Actinin - Wikipedia
Actinin is a microfilament protein. Alpha-actinin-1 is necessary for the attachment of actin myofilaments to the Z-lines in ... Both ends of the rod-shaped alpha-actinin dimer contain actin-binding domains. Mutations in ACTN4 can cause the kidney disease ... ISBN 978-0-08-092427-4. Actinin at the US National Library of Medicine Medical Subject Headings (MeSH) v t e (Articles with ... The non-sarcomeric alpha-actinins, encoded by ACTN1 and ACTN4, are widely expressed. ACTN2 expression is found in both cardiac ...
Actn1 actinin, alpha 1 [Mus musculus (house mouse)] - Gene - NCBI
alpha-actinin-1. Names. F-actin cross-linking protein. alpha actinin 1a. non-muscle alpha-actinin-1. ... actinin, alpha 1provided by MGI. Primary source. MGI:MGI:2137706 See related. Ensembl:ENSMUSG00000015143 AllianceGenome:MGI: ... XM_030246514.2 → XP_030102374.1 alpha-actinin-1 isoform X2. Conserved Domains (4) summary. cd00051. Location:507 → 596. EFh; EF ... XM_036157161.1 → XP_036013054.1 alpha-actinin-1 isoform X3. Conserved Domains (4) summary. cd00051. Location:475 → 564. EFh; EF ...
How flexible is α-actinin's rod domain?
"α-actinin, an actin binding protein, plays a key role in cell migration, cross-links actin filaments in the Z-disk, and is a ... α-actinin, an actin binding protein, plays a key role in cell migration, cross-links actin filaments in the Z-disk, and is a ... α-actinin rod domain, α-spectrin, Steered Molecular Dynamics. Cite This Article. Zaman, M. H., Kaazempur-Mofrad, M. R. (2004). ... How flexible is α-actinins rod domain?. Muhammad H. Zaman1, Mohammad R. Kaazempur-Mofrad2 1. Whitehead Institute, 9 Cambridge ...
Alpha-actinin of the chlorarchiniophyte Bigelowiella natans.
Similar to human α-actinin, calcium-binding occurs to the most N-terminal EF-hand motif in the calmodulin-like C-terminal ... Analysis of the primary sequence indicate that this protein has the same domain structure as other α-actinins, a N-terminal ... The results indicate that this Bigelowiella protein is a proper α-actinin, with all common characteristics of a typical α- ... The genome of the chlorarchiniophyte Bigelowiella natans codes for a protein annotated as an α-actinin-like protein. ...
Pediatric Nephrotic Syndrome: Practice Essentials, Background, Pathophysiology
alpha-Actinin monoclonal antibody Western, Immunofluorescence A5044
Monoclonal Anti-α-Actinin antibody produced in mouse (monoclonal BM-75.2, ascites fluid); Suitable for indirect ... α-actinin is a 100kD actin binding protein found in muscle as well as non-muscle cells. In smooth muscle, α-actinin is ... The gene encoding α-actinin is mapped to the human chromosome 11q13.2. α-actinin belongs to the spectrin protein superfamily.[ ... Monoclonal anti-α-actinin antibody (diluted 1: 500) can be used in native protein binding assays[. 69. ]. It can also be used ...
Crucial functions of alpha-actinin 2 in the embryonic heart - SCNi
Interaction of annexin A6 with alpha actinin in cardiomyocytes | BMC Molecular and Cell Biology | Full Text
... actinin antibody showed the presence of α-actinin in the immunoblot which was absent when GST-N terminus deleted annexin A6 was ... In overall, the present study demonstrated for the first time that annexin A6 physically interacts with sarcomeric α-actinin ... Double immunofluorescent staining of cardiomyocytes with anti annexin A6 and anti sarcomeric α-actinin antibodies showed ... as revealed by α-actinin immunostaining in shRNA treated cells. ... From: Interaction of annexin A6 with alpha actinin in ...
talks.cam : Alpha-actinin dynamically anchors PSD-95 and AMPARs at postsynaptic sites
Purification and properties of [alpha]-actinin from rabbit striated muscle
Science Clips - Monday, July 28, 2014
Cardiomyocyte immunofluorescence protocol
Procedure describing a suggested protocol for sarcomeric actinin, ANP, ER and ERb fluorescent staining of cardiomyocytes. ... The α-actinin stain is stable for quite some time but the ANP stain tends to bleach out within about 1-2 weeks. ... Treat cells with antibodies for α-actinin eg. alpha sarcomeric actin antibody [alpha Sr-1] (ab28052) and ANP diluted in the ... Treat cells with antibodies for α-actinin (1/200 dilution) diluted in the blocking buffer as above for 1 hour. ...
Proteomes | Free Full-Text | Differential Proteome Analysis of Extracellular Vesicles from Breast Cancer Cell Lines by...
Affixin interacts with a-actinin and mediates integrin signaling for reorganization of F-actin induced by initial cell...
A "human knockout" model to investigate the influence of the α-actinin-3 protein on exercise-induced mitochondrial adaptations ...
View source for Dynamic Viscoelasticity of Actin Cross-Linked with Wild-Type and Disease-Causing Mutant α-Actinin-4 - Soft...
... wild-type α-actinin-4, and (C) mutant α-actinin-4. Scale bar is ,math>40\mu m,/math>]] By performing rheological experiments, ... View source for Dynamic Viscoelasticity of Actin Cross-Linked with Wild-Type and Disease-Causing Mutant α-Actinin-4. ← Dynamic ... Of particular interest is a cross-linker called α-actinin-4. This cross-linker is present naturally in podocytes, the kidney ... By examining the relaxation frequency of the wild-type and mutant α-actinin-4 networks at various temperatures, the authors ...
Anti-ACTN4 Mouse mAb (4D10) | CB1024
Anti-Actinin α4. Application Data. Detection of rat ACTN4 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. ... Anti-Actinin α4. Application. ELISA (see coments). Immunoblotting (1-5 µg/ml). Immunocytochemistry (20 µg/ml). Paraffin ... Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal ... Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the ...
Park DK[Author] - Search Results - PubMed
Distinct Dendritic Arborization and In Vivo Firing Patterns of Parvalbumin-Expressing Basket Cells in the Hippocampal Area CA3 ...
Is athletic performance determined by genetics?: MedlinePlus Genetics
A variant in this gene, called R577X, leads to production of an abnormally short α-actinin-3 protein that is quickly broken ... The ACTN3 gene provides instructions for making a protein called alpha (α)-actinin-3, which is predominantly found in fast- ... These individuals have a complete absence of α-actinin-3, which appears to reduce the proportion of fast-twitch muscle fibers ...
The actin-ECM (extracellular matrix) linkage defects caused by mutations in the Kindlin1 gene are mediated by actinin alpha<...
The actin-ECM (extracellular matrix) linkage defects caused by mutations in the Kindlin1 gene are mediated by actinin alpha. / ... The actin-ECM (extracellular matrix) linkage defects caused by mutations in the Kindlin1 gene are mediated by actinin alpha. ... The actin-ECM (extracellular matrix) linkage defects caused by mutations in the Kindlin1 gene are mediated by actinin alpha. ... title = "The actin-ECM (extracellular matrix) linkage defects caused by mutations in the Kindlin1 gene are mediated by actinin ...
Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin.
SASDJL6 - Sarcomeric F-actin crosslinking protein α-actinin-2 (spectrin repeat rod domain, rod-α-actinin-2) ... SASDJN6 - Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (rod-α-actinin-2/Δ91-FATZ-1) ... SASDJP6 - Sarcomeric fuzzy α-actinin-2/FATZ-1 complex (hd-α-actinin-2/Δ91-FATZ-1) ... Rod domain of α-actinin-2 dimer, 112 kDa Homo sapiens protein. Buffer:. 50 mM Tris-HCl 150 mM NaCl, pH: 7.5. ...
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response to denervation involved in regulation of muscle adaptation - Ontology Report - Rat Genome Database
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alpha-actinin ,α-actinin,. Alpha-Aktinin {n}. ,α-Aktinin, med.. alpha-synucleinopathies ,α-synucleinopathies,. Alpha- ... α-actinin. α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic. α-Aminobutyric. α-Aminobutyric acid. α-amylase. α-cells. α Cen. α ... alpha-actinin ,α-actinin,. Alpha-Actinin {n}. ,α-Actinin, biochem.. alpha-amylase ,α-amylase,. Alpha-Amylase {f}. ,α-Amylase, ...
ACTN12
- The non-sarcomeric alpha-actinins, encoded by ACTN1 and ACTN4, are widely expressed. (wikipedia.org)
- Orthologous to human ACTN1 (actinin alpha 1). (nih.gov)
Actn39
- The ACTN3 gene provides instructions for making a protein called alpha (α)-actinin-3, which is predominantly found in fast-twitch muscle fibers. (medlineplus.gov)
- A common variant of the ACTN3 gene, R577X, results in complete deficiency of the α-actinin-3 protein in the fast skeletal muscle fibers of more than a billion humans worldwide. (lww.com)
- One gene potentially associated with human physical performance is the ACTN3 gene, which encodes the protein α-actinin-3. (lww.com)
- This variation creates two different versions of the ACTN3 gene, both of which are common in the general population: the 577R version (or allele) is the normal, functional version of the gene, whereas the 577X allele contains a sequence change that completely prevents the production of functional α-actinin-3 protein ( 12 ). (lww.com)
- In its most common variation, which accounts for about 80% of the Australian population, ACTN3 encodes for a protein called alpha-actinin-3. (cavemanpower.com)
- They found the muscle metabolism of the mice without the actinin protein was more efficient: the mice were able to run, on average, 33 per cent further before reaching exhaustion than mice with the normal ACTN3 gene. (cavemanpower.com)
- Alpha-actinin-3 (ACTN3) is a protein found mainly in fast glycolytic muscle fibers. (gentrend.cz)
- Picture this, individuals with a specific variant of the ACTN3 gene, which is one of the most studied fitnessgenes have muscles which contract powerfully at high speeds because they secrete a specific protein called alpha actinin. (xcode.life)
- A special emphasis was placed on those candidate polymorphisms that have been more extensively studied, i.e. angiotensin-converting enzyme (ACE) gene I/D, α-actinin-3 (ACTN3) R577X, and myostatin (MSTN) K153R, among others. (universidadeuropea.com)
Antibodies10
- Treat cells with antibodies for α-actinin eg. (abcam.com)
- Treat cells with antibodies for α-actinin (1/200 dilution) diluted in the blocking buffer as above for 1 hour. (abcam.com)
- Pathogenic anti-DNA antibodies are able to interact at the tissue level with alpha actinin in the renal glomeruli. (immpact-international.org)
- Cross-reactivity of human lupus anti-DNA antibodies with alpha-actinin and nephritogenic potential. (anapatterns.org)
- Anti-alpha-actinin antibodies are part of the anti-cell membrane antibody spectrum that characterize patients with lupus nephritis. (anapatterns.org)
- Anti-alpha-actinin antibodies in relation to new-onset systemic lupus erythematosus and lupus nephritis. (anapatterns.org)
- Anti-alpha-actinin antibodies cross-react with anti-ssDNA antibodies in active autoimmune hepatitis. (anapatterns.org)
- Anti-alpha actinin antibodies as new predictors of response to treatment in autoimmune hepatitis type 1. (anapatterns.org)
- The differentiation potential of EBs into cardiomyocyte-like cells was confirmed by using antibodies against sarcomeric α-actinin, cardiac troponin T and connexin 43. (sciendo.com)
- C) Immunolabeling of hiPSC-CMs with anti-cTNT (green) and anti-sarcomeric -actinin (reddish colored) antibodies with Hoechst 33342 staining. (cox2-inhibitor.com)
Proteins11
- Staining indicates cardiac sarcomere proteins alpha actinin and cardiac troponin T, in addition to nuclear stain DAPI. (eurekalert.org)
- Actin binding domains present in duplicate at the N-termini of spectrin-like proteins (including dystrophin, alpha-actinin). (embl.de)
- A number of actin-binding proteins, including spectrin, alpha-actinin and fimbrin, contain a 250 amino acid stretch called the actin binding domain (ABD). (embl.de)
- We found evidence that defined proteolytic cleavage results in various proteoforms of important podocyte proteins, including those of podocin, nephrin, neph1, alpha-actinin-4, and vimentin. (uni-koeln.de)
- The N-terminal actin-binding region consists of two calponin homology domains and is related to the actin-binding domains of a superfamily of proteins including alpha-actinin, spectrin and fimbrin. (rcsb.org)
- Specifically, Actin is assembled into contractile stress fibers, which are organized structures consisting of parallel Actin fibers with periodic cross-linking proteins such as alpha-Actinin and Myosin II motor proteins. (rndsystems.com)
- Our approach is two-fold: first, we conducted biochemical studies focused on the interactions of actin filaments and the actin binding proteins ARP2/3, alpha-actinin, dynamin2, and cortactin and second, we developed a computational model to validate experimental hypotheses for actin filament crosslinking to promote filament bundling. (jmu.edu)
- The sarcomeres contain a number of proteins, among them are alpha actinin, which is the major constituent of the Z-band, and actin and myosin, which are the major components of the thin and thick filaments, respectively. (medscape.com)
- About half of the genes encode sarcomeric proteins including α- cardiac actin , myosin heavy chain , troponin , tropomyosin , metavinculin, α- actinin and therefore overlap with those in HCM. (dictionary.education)
- It has been shown that this up-regulation of FA proteins, such as vinculin, talin, paxillin, and actin-crosslinking -actinin, causes astrocytes activation and increases the expression of intermediates filaments, including Glial Fibrillary Acidic Protein, vimentin, and nestin [176]. (aqualogy.org)
- The abstract states, "Glutathione-S-transferase (GST) pulldown assays were used to show that RPTPrho interacts with several adherens junctional proteins in brain, including E-cadherin, N-cadherin, VE-cadherin (cadherin-5), desmoglein, alpha, beta [Ctnnb1] and gamma [Jup] catenin, p120(ctn) and alpha-actinin. (geneontology.org)
Alpha28
- Alpha-actinin-1 is necessary for the attachment of actin myofilaments to the Z-lines in skeletal muscle cells, and to the dense bodies in smooth muscle cells. (wikipedia.org)
- Both ends of the rod-shaped alpha-actinin dimer contain actin-binding domains. (wikipedia.org)
- Alpha-actinin of the chlorarchiniophyte Bigelowiella natans. (scilifelab.se)
- Proteolytic cleavage of alpha-actinin by calpain in T cells stimulated with anti-CD3 monoclonal antibody. (jimmunol.org)
- alpha-actinin-3 and performance. (cdc.gov)
- Among elite power athletes the alpha-actinin-3 protein is nearly always present. (cavemanpower.com)
- Those with the variant form of the gene, about 20 per cent of the population, do not make the alpha-actinin-3 protein. (cavemanpower.com)
- Researchers developed a strain of mice that were completely deficient in alpha-actinin-3. (cavemanpower.com)
- Most Africans have alpha-actinin-3, it's the normal ancestral state. (cavemanpower.com)
- Myotilin forms homodimers and binds to alpha-actinin, F-actin, and filamin C. (yeastrc.org)
- Cultured chicken embryonic cardiomyocyte fixed and stained for alpha-actinin using a monoclonal antibody and Alexa-546 as a secondary fluorescent antibody. (cellimagelibrary.org)
- In cardiac and skeletal muscle cells, Myozenin 2 binds calcineurin to alpha-actinin at the Z-line of the sarcomere. (fishersci.no)
- Alpha-actinin was positively identified using electrophoretic techniques and confirmed by tandem mass spectroscopy. (canada.ca)
- Additionally, cortactin decreases the association of alpha-actinin with bundled actin filaments. (jmu.edu)
- We hypothesize that the transition from a branched to bundled F-actin network architecture involves balancing the activities of two competing filament crosslinkers: dynamin2 and cortactin vs. alpha-actinin. (jmu.edu)
- We will test this hypothesis by creating an emergent Monte Carlo model in MATLAB that simulates filament crosslinking in the presence of alpha-actinin, cortactin and dynamin2. (jmu.edu)
- e.g. anti-alpha-actinin, anti-vinculin. (anapatterns.org)
- Double reactivity against actin and alpha-actinin defines a severe form of autoimmune hepatitis type 1. (anapatterns.org)
- Interaction of alpha-actinin and nebulin in vitro. (mpg.de)
- actinin alpha 1 [Source:HGNC Symbo. (gsea-msigdb.org)
- Synaptopodin-2 has other known binding partners such as filamin and [alpha]-actinin (Weins, et al. (ecu.edu)
- This genetic marker controls production of the Alpha-Actinin-3 protein in fast-twitch muscle fibers. (a7.co)
- Actin/alpha-actinin-dependent transport of AMPA receptors in dendritic spines: role of the PDZ-LIM protein RIL. (mpg.de)
- Here, it colocalises with metavinculin, vinculin and alpha-actinin, all of which have biochemically been identified as raver1 ligands. (helmholtz-hzi.de)
- Research has shown that individuals with the gene type R produce a specific protein (alpha-actinin-3) that is exclusively found in the fast-twitch muscle fibers , used during explosive activities, such as sprinting or weight-lifting. (freeletics.com)
- genetically encoded FRET-based force sensors inserted in F-actin and alpha-actinin are used to measure the propagation of mechanical signals to the cell cytoskeleton, while fluorescence microscopy with single-molecule sensitivity can be used with a huge array of biochemical and genetic sensors. (epj.org)
- We describe the details of the setup implementation, the calibration of the basic components and preliminary characterization of actin and alpha-actinin FRET-based force sensors. (epj.org)
- The apparent reason for this: the loss of ACNT3's protein was compensated by a different protein, called alpha-actinin-2, which shifted muscle metabolism towards a smoother, more efficient, aerobic pathway. (blogspot.com)
ACTN41
- In the reanalysis of the National Cancer Institute Canadian Clinical Trial Group JBR.10, which led to the adoption of adjuvant cisplatin-based chemotherapy for patients with resected stages-IB to IIIA non-small cell lung cancer (NSCLC), we demonstrated a significant clinical benefit of adjuvant chemotherapy for overall survival only in the actinin-4 (ACTN4) high expression group. (nmsbiomarker.com)
Spectrin2
- α-actinin rod domain, α-spectrin, Steered Molecular Dynamics. (techscience.com)
- α-actinin belongs to the spectrin protein superfamily. (sigmaaldrich.com)
Hardening in F-actin1
- 2] H. Lopez, J.F. Rodriguez, Microstructural model for cyclic hardening in F-actin networks crosslinked by -actinin. (polimi.it)
Skeletal muscle3
- α-Actinin involvement in Z-disk assembly during skeletal muscle C2C12 cells in vitro differentiation. (nih.gov)
- In smooth muscle, α-actinin is identified in dense bodies and plaques characteristic of tissues whereas it is associated with z-discs that define muscle sarcomas in normal skeletal muscle. (sigmaaldrich.com)
- Remarkably, the remaining 18% of the healthy European population-and more than a billion people worldwide-have two copies of the nonfunctional 577X variant (the XX genotype), resulting in complete deficiency of α-actinin-3 protein in their skeletal muscle ( 9 ). (lww.com)
Fast-twitch muscle1
- These individuals have a complete absence of α-actinin-3, which appears to reduce the proportion of fast-twitch muscle fibers and increase the proportion of slow-twitch fibers in the body. (medlineplus.gov)
Myosin4
- Top row movies: Z-stacks of live hiPS cells expressing mEGFP-tagged ß-actin (left), α-actinin (center) and non-muscle myosin heavy chain IIB (right) imaged on a spinning-disk confocal microscope. (allencell.org)
- Representative images of mEGFP tagged ß-actin (left), α-actinin (center) and non-muscle myosin heavy chain IIB (right) from the bottom (top row) and tops (bottom row) of cells. (allencell.org)
- Myosin IIB localization is similar to that of actin and α -actinin, residing in prominent filaments primarily at the bottom and top of cells. (allencell.org)
- In summary, α-actinin localizes to a subset of actin (e.g. the actin bundles) and myosin IIB to a yet smaller subset (actomyosin bundles) as expected. (allencell.org)
Antibody2
- Monoclonal anti-α-actinin antibody can be used in immunoblotting to study the membrane anchorage sites and immunochemical identification of α-actinin. (sigmaaldrich.com)
- Mouse anti-α-actinin antibody reacts specifically with chicken fibroblasts. (sigmaaldrich.com)
R577X2
- A variant in this gene, called R577X, leads to production of an abnormally short α-actinin-3 protein that is quickly broken down. (medlineplus.gov)
- The gene for this protein occurs in two variants, R and X. Variant X (or more specifically, R577X) carries information for the cell causing muscle protein α-actinin-3 deficiency. (gentrend.cz)
Fibers1
- The α-actinin-2 protein is present predominantly in the aerobically working muscle fibers, giving the muscle greater endurance. (gentrend.cz)
Talin1
- U.S. are exposed to attaching/effacing enteropathogens merized actin, -actinin, talin, and ezrin (2). (cdc.gov)
Cardiomyocytes1
- Immunofluorescence of cardiomyocytes from neonatal and adult mouse hearts was performed using monoclonal mouse anti-α actinin (clone EA-53) at 1:1000. (sigmaaldrich.com)
Vitro1
- Quantitative mapping of N-termini demonstrated perturbation of protease action during podocyte injury in vitro, including diminished proteolysis of a-actinin-4. (uni-koeln.de)
Mutations1
- Mutations in the α-actinin gene is observed in heterogeneous hypertrophic cardiomyopathy and in juvenile onset atrial fibrillation. (sigmaaldrich.com)
Sarcomere1
- Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin. (sasbdb.org)
ELISA1
- Serologic evidence of a history of trichomonosis was assessed by a recombinant Trichomonas vaginalis α-actinin IgG ELISA. (aacrjournals.org)
Localization2
- It can also be used in immunofluorescence to study the localization of α-actinin in cultured cells and tissues. (sigmaaldrich.com)
- α -actinin localization is almost identical to ß-actin. (allencell.org)
Molar2
- Figure 3: Frequency dependence of elastic modulus (solid) and viscous modulus (open) for different concentrations of (A) wild-type and (B) mutant α-actinin-4 for different molar concentrations of cross-linker. (harvard.edu)
- actinin bound synthetic astaxanthin in a molar ratio of 1.11:1.00. (canada.ca)
Rabbit2
- using ApoE (pan) (D7I9N) Rabbit mAb (upper) and α-Actinin (D6F6) XP ® Rabbit mAb #6487 (lower). (cellsignal.com)
- Western blot analysis of extracts from various cell lines using ARID1B/BAF250B (E9J4T) Rabbit mAb (upper) and α-Actinin (D6F6) Rabbit mAb #6487 (lower). (cellsignal.com)
Human2
- Similar to human α-actinin, calcium-binding occurs to the most N-terminal EF-hand motif in the calmodulin-like C-terminal domain. (scilifelab.se)
- The gene encoding α-actinin is mapped to the human chromosome 11q13.2. (sigmaaldrich.com)
Periodic2
- The image shows a single isolated Wistar rat cardiomyocyte stained against α-actinin, displaying its periodic structure localized at the ends of sarcomeres (Z-discs). (tocris.com)
- These rods contain the Z-line protein, α-actinin, that can be effectively stained in skeletal muscles using Gomori or Masson trichrome and negatively stained with periodic acid-Schiff. (scielo.org.za)
Deficiency1
- This is not a pathogenic issue as its deficiency is effectively compensated by the related protein α-actinin-2. (gentrend.cz)
Interaction1
- Affixin interacts with a-actinin and mediates integrin signaling for reorganization of F-actin induced by initial cell-substrate interaction. (nii.ac.jp)
Muscle4
- α-actinin, an actin binding protein, plays a key role in cell migration, cross-links actin filaments in the Z-disk, and is a major component of contractile muscle apparatus. (techscience.com)
- α-actinin is a 100kD actin binding protein found in muscle as well as non-muscle cells. (sigmaaldrich.com)
- α−actinin regulates muscle contraction and cytoskeletal organization. (sigmaaldrich.com)
- The precise functions of α-actinin-3 are still unknown but are likely to include a structural role in the maintenance of muscle mechanical integrity and possibly other functions related to muscle signaling and metabolism ( 8 ). (lww.com)
Focal1
- Additionally, I will be investigating the roles of focal adhesion molecule α-actinin in neutrophil recruitment and arrest. (brown.edu)
Calcium1
- has been postulated that decreased mobilization of calcium actinin are distributed over the lateral cricoarytenoid m. (psm.edu)
Bundles2
- α -actinin crosslinks actin filaments, forming actin bundles. (allencell.org)
- The main difference is that α -actinin localizes less strongly to the lamellipodia at the protruding edges at the bottom of the cells, where there are fewer bundles. (allencell.org)
Promotes1
- α-Actinin-1 promotes activity of the L-type Ca2+ channel Cav 1.2. (ucdavis.edu)
Domain1
- Analysis of the primary sequence indicate that this protein has the same domain structure as other α-actinins, a N-terminal actin-binding domain and a C-terminal calmodulin-like domain. (scilifelab.se)