A protein factor that regulates the length of R-actin. It is chemically similar, but immunochemically distinguishable from actin.
A form of creatine kinase found in the BRAIN.
Monomeric subunits of primarily globular ACTIN and found in the cytoplasmic matrix of almost all cells. They are often associated with microtubules and may play a role in cytoskeletal function and/or mediate movement of the cell or the organelles within the cell.
The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.
Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.
A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.
The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Kidney disorders with autosomal dominant inheritance and characterized by multiple CYSTS in both KIDNEYS with progressive deterioration of renal function.
A subgroup of TRP cation channels that are widely expressed in various cell types. Defects are associated with POLYCYSTIC KIDNEY DISEASES.
Hereditary diseases that are characterized by the progressive expansion of a large number of tightly packed CYSTS within the KIDNEYS. They include diseases with autosomal dominant and autosomal recessive inheritance.
Any fluid-filled closed cavity or sac that is lined by an EPITHELIUM. Cysts can be of normal, abnormal, non-neoplastic, or neoplastic tissues.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
A broad group of eukaryotic six-transmembrane cation channels that are classified by sequence homology because their functional involvement with SENSATION is varied. They have only weak voltage sensitivity and ion selectivity. They are named after a DROSOPHILA mutant that displayed transient receptor potentials in response to light. A 25-amino-acid motif containing a TRP box (EWKFAR) just C-terminal to S6 is found in TRPC, TRPV and TRPM subgroups. ANKYRIN repeats are found in TRPC, TRPV & TRPN subgroups. Some are functionally associated with TYROSINE KINASE or TYPE C PHOSPHOLIPASES.
A genetic disorder with autosomal recessive inheritance, characterized by multiple CYSTS in both KIDNEYS and associated LIVER lesions. Serious manifestations are usually present at BIRTH with high PERINATAL MORTALITY.
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
A family of crosslinking filament proteins encoded by distinct FLN genes. Filamins are involved in cell adhesion, spreading, and migration, acting as scaffolds for over 90 binding partners including channels, receptors, intracellular signaling molecules and transcription factors. Due to the range of molecular interactions, mutations in FLN genes result in anomalies with moderate to lethal consequences.
Reagents with two reactive groups, usually at opposite ends of the molecule, that are capable of reacting with and thereby forming bridges between side chains of amino acids in proteins; the locations of naturally reactive areas within proteins can thereby be identified; may also be used for other macromolecules, like glycoproteins, nucleic acids, or other.
Proteins which participate in contractile processes. They include MUSCLE PROTEINS as well as those found in other cells and tissues. In the latter, these proteins participate in localized contractile events in the cytoplasm, in motile activity, and in cell aggregation phenomena.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The repeating contractile units of the MYOFIBRIL, delimited by Z bands along its length.
Proteins prepared by recombinant DNA technology.
A giant elastic protein of molecular mass ranging from 2,993 kDa (cardiac), 3,300 kDa (psoas), to 3,700 kDa (soleus) having a kinase domain. The amino- terminal is involved in a Z line binding, and the carboxy-terminal region is bound to the myosin filament with an overlap between the counter-connectin filaments at the M line.
The long cylindrical contractile organelles of STRIATED MUSCLE cells composed of ACTIN FILAMENTS; MYOSIN filaments; and other proteins organized in arrays of repeating units called SARCOMERES .
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
A signal transducer and activator of transcription that mediates cellular responses to INTERLEUKIN-6 family members. STAT3 is constitutively activated in a variety of TUMORS and is a major downstream transducer for the CYTOKINE RECEPTOR GP130.
A species of TRICHOMONAS that produces a refractory vaginal discharge in females, as well as bladder and urethral infections in males.
A publication issued at stated, more or less regular, intervals.
The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)
Inflammation of the vagina, marked by a purulent discharge. This disease is caused by the protozoan TRICHOMONAS VAGINALIS.
Individual's rights to obtain and use information collected or generated by others.
Infections in birds and mammals produced by various species of Trichomonas.
A high molecular weight (220-250 kDa) water-soluble protein which can be extracted from erythrocyte ghosts in low ionic strength buffers. The protein contains no lipids or carbohydrates, is the predominant species of peripheral erythrocyte membrane proteins, and exists as a fibrous coating on the inner, cytoplasmic surface of the membrane.
A muscle protein localized in surface membranes which is the product of the Duchenne/Becker muscular dystrophy gene. Individuals with Duchenne muscular dystrophy usually lack dystrophin completely while those with Becker muscular dystrophy have dystrophin of an altered size. It shares features with other cytoskeletal proteins such as SPECTRIN and alpha-actinin but the precise function of dystrophin is not clear. One possible role might be to preserve the integrity and alignment of the plasma membrane to the myofibrils during muscle contraction and relaxation. MW 400 kDa.
An autosomally-encoded 376-kDa cytoskeletal protein that is similar in structure and function to DYSTROPHIN. It is a ubiquitously-expressed protein that plays a role in anchoring the CYTOSKELETON to the PLASMA MEMBRANE.
A group of proteins that associate with DYSTROPHIN at the CELL MEMBRANE to form the DYSTROPHIN-ASSOCIATED PROTEIN COMPLEX.

Gamma-Actinin, a new regulatory protein from rabbit skeletal muscle. I. Purification and characterization. (1/917)

A new regulatory protein which we have designated as gamma-actinin has been isolated from native thin filaments of rabbit skeletal muscle. Depolymerized native thin filaments were fractionated by salting out with ammonium sulfate, and the precipitates obtained at 40--60% ammonium sulfate saturation were further subjected to DEAE-Sephadex and Sephadex G-200 column chromatography. The purified gamma-actinin was shown to have a chain weight of 35,000 daltons and had a strong inhibitory action on the polymerization of G-actin. The results of amino acid analysis indicated a unique amino acid composition of gamma-actinin as compared with other structural proteins of muscle. Non-polar and neutral amino acid residues were abundant. One cysteine residue was contained per one molecule of gamma-actinin and played a critical role in the maintenance of the inhibitory activity. Pelleting of gamma-actinin with F-actin showed that gamma-actinin binds to F-action.  (+info)

Polypyrimidine tract binding protein functions as a repressor to regulate alternative splicing of alpha-actinin mutally exclusive exons. (2/917)

The smooth muscle (SM) and nonmuscle (NM) isoforms of alpha-actinin are produced by mutually exclusive splicing of an upstream NM exon and a downstream SM-specific exon. A rat alpha-actinin genomic clone encompassing the mutually exclusive exons was isolated and sequenced. The SM exon was found to utilize two branch points located 382 and 386 nucleotides (nt) upstream of the 3' splice site, while the NM exon used a single branch point 191 nt upstream. Mutually exclusive splicing arises from the proximity of the SM branch points to the NM 5' splice site, and this steric repression could be relieved in part by the insertion of spacer elements. In addition, the SM exon is repressed in non-SM cells and extracts. In vitro splicing of spacer-containing transcripts could be activated by (i) truncation of the transcript between the SM polypyrimidine tract and exon, (ii) addition of competitor RNAs containing the 3' end of the actinin intron or regulatory sequences from alpha-tropomyosin (TM), and (iii) depletion of the splicing extract by using biotinylated alpha-TM RNAs. A number of lines of evidence point to polypyrimidine tract binding protein (PTB) as the trans-acting factor responsible for repression. PTB was the only nuclear protein observed to cross-link to the actinin RNA, and the ability of various competitor RNAs to activate splicing correlated with their ability to bind PTB. Furthermore, repression of alpha-actinin splicing in the nuclear extracts depleted of PTB by using biotinylated RNA could be specifically restored by the addition of recombinant PTB. Thus, alpha-actinin mutually exclusive splicing is enforced by the unusual location of the SM branch point, while constitutive repression of the SM exon is conferred by regulatory elements between the branch point and 3' splice site and by PTB.  (+info)

Tropomodulin assembles early in myofibrillogenesis in chick skeletal muscle: evidence that thin filaments rearrange to form striated myofibrils. (3/917)

Actin filament lengths in muscle and nonmuscle cells are believed to depend on the regulated activity of capping proteins at both the fast growing (barbed) and slow growing (pointed) filament ends. In striated muscle, the pointed end capping protein, tropomodulin, has been shown to maintain the lengths of thin filaments in mature myofibrils. To determine whether tropomodulin might also be involved in thin filament assembly, we investigated the assembly of tropomodulin into myofibrils during differentiation of primary cultures of chick skeletal muscle cells. Our results show that tropomodulin is expressed early in differentiation and is associated with the earliest premyofibrils which contain overlapping and misaligned actin filaments. In addition, tropomodulin can be found in actin filament bundles at the distal tips of growing myotubes, where sarcomeric alpha-actinin is not always detected, suggesting that tropomodulin caps actin filament pointed ends even before the filaments are cross-linked into Z bodies by alpha-actinin. Tropomodulin staining exhibits an irregular punctate pattern along the length of premyofibrils that demonstrate a smooth phalloidin staining pattern for F-actin. Strikingly, the tropomodulin dots often appear to be located between the closely spaced, dot-like Z bodies that are stained for (&agr;)-actinin. Thus, in the earliest premyofibrils, the pointed ends of the thin filaments are clustered and partially aligned with respect to the Z bodies (the location of the barbed filament ends). At later stages of differentiation, the tropomodulin dots become aligned into regular periodic striations concurrently with the appearance of striated phalloidin staining for F-actin and alignment of Z bodies into Z lines. Tropomodulin, together with the barbed end capping protein, CapZ, may function from the earliest stages of myofibrillogenesis to restrict the lengths of newly assembled thin filaments by capping their ends; thus, transitions from nonstriated to striated myofibrils in skeletal muscle are likely due principally to filament rearrangements rather than to filament polymerization or depolymerization. Rearrangements of actin filaments capped at their pointed and barbed ends may be a general mechanism by which cells restructure their actin cytoskeletal networks during cell growth and differentiation.  (+info)

An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment. (4/917)

The LIM domain protein zyxin is a component of adherens type junctions, stress fibers, and highly dynamic membrane areas and appears to be involved in microfilament organization. Chicken zyxin and its human counterpart display less than 60% sequence identity, raising concern about their functional identity. Here, we demonstrate that human zyxin, like the avian protein, specifically interacts with alpha-actinin. Furthermore, we map the interaction site to a motif of approximately 22 amino acids, present in the N-terminal domain of human zyxin. This motif is both necessary and sufficient for alpha-actinin binding, whereas a downstream region, which is related in sequence, appears to be dispensable. A synthetic peptide comprising human zyxin residues 21-42 specifically binds to alpha-actinin in solid phase binding assays. In contrast to full-length zyxin, constructs lacking this motif do not interact with alpha-actinin in blot overlays and fail to recruit alpha-actinin in living cells. When zyxin lacking the alpha-actinin binding site is expressed as a fusion protein with green fluorescent protein, association of the recombinant protein with stress fibers is abolished, and targeting to focal adhesions is grossly impaired. Our results suggest a crucial role for the alpha-actinin-zyxin interaction in subcellular zyxin localization and microfilament organization.  (+info)

Viscoelastic properties of f-actin, microtubules, f-actin/alpha-actinin, and f-actin/hexokinase determined in microliter volumes with a novel nondestructive method. (5/917)

A nondestructive method to determine viscoelastic properties of gels and fluids involves an oscillating glass fiber serving as a sensor for the viscosity of the surrounding fluid. Extremely small displacements (typically 1-100 nm) are caused by the glass rod oscillating at its resonance frequency. These displacements are analyzed using a phase-sensitive acoustic microscope. Alterations of the elastic modulus of a fluid or gel change the propagation speed of a longitudinal acoustic wave. The system allows to study quantities as small as 10 microliters with temporal resolution >1 Hz. For 2-100 microM f-actin gels a final viscosity of 1.3-9.4 mPa s and a final elastic modulus of 2.229-2.254 GPa (corresponding to 1493-1501 m/s sound velocity) have been determined. For 10- to 100-microM microtubule gels (native, without stabilization by taxol), a final viscosity of 1.5-124 mPa s and a final elastic modulus of 2.288-2. 547 GPa (approximately 1513-1596 m/s) have been determined. During polymerization the sound velocity in low-concentration actin solutions increased up to +1.3 m/s (approximately 1.69 kPa) and decreased up to -7 m/s (approximately 49 kPa) at high actin concentrations. On polymerization of tubulin a concentration-dependent decrease of sound velocity was observed, too (+48 to -12 m/s approximately 2.3-0.1 MPa, for 10- to 100-microM tubulin). This decrease was interpreted by a nematic phase transition of the actin filaments and microtubules with increasing concentration. 2 mM ATP (when compared to 0.2 mM ATP) increased polymerization rate, final viscosity and elastic modulus of f-actin (17 microM). The actin-binding glycolytic enzyme hexokinase also accelerated the polymerization rate and final viscosity but elastic modulus (2.26 GPa) was less than for f-actin polymerized in presence of 0.2 mM ATP (2.28 GPa).  (+info)

Cardiac microvascular endothelial cells express alpha-smooth muscle actin and show low NOS III activity. (6/917)

We established a culture system of porcine coronary microvascular endothelial cells (MVEC) with high cellular yield and purity >98%. Endothelial origin was confirmed by immunostaining, immunoblotting and fluorescence-activated cell sorter (FACS) analysis using low-density lipoprotein uptake, CD31, von Willebrand factor, and the lectin Dolichos biflorus agglutinin. MVEC were positive for alpha-smooth muscle actin in culture and in myocardium, as confirmed by FACS. Of the primary MVEC, approximately 30% expressed nitric oxide synthase (NOS) III in numbers decreasing from the first passage (6 +/- 1%) to the second passage (4 +/- 1%; P < 0.001 vs. primary isolates), whereas approximately 100% of aortic endothelial cells (AEC) expressed NOS III. In AEC, NOS III activity (pmol citrulline. mg protein-1. min-1) was 80 +/- 10 and was nearly abolished in the absence of calcium (5 +/- 1, P < 0.001). In primary MVEC, however, NOS III activity in the presence and absence of calcium was 20 +/- 4 and 25 +/- 5, respectively. We conclude that cardiac MVEC, in contrast to AEC, contain alpha-smooth muscle actin, show low-grade NOS III activity, and provide a suitable in vitro system for the study of endothelial pathophysiology.  (+info)

Myotilin, a novel sarcomeric protein with two Ig-like domains, is encoded by a candidate gene for limb-girdle muscular dystrophy. (7/917)

The striated muscle sarcomeres are highly organized structures composed of actin (thin) and myosin (thick) filaments that slide past each other during contraction. The integrity of sarcomeres is controlled by a set of structural proteins, among which are titin, a giant molecule that contains several immunoglobulin (Ig)-like domains and associates with thin and thick filaments, and [alpha]-actinin, an actin cross-linking protein. Mutations in several sarcomeric and sarcolemmal proteins have been shown to result in muscular dystrophy and cardiomyopathy. On the other hand, the disease genes underlying several disease forms remain to be identified. Here we describe a novel 57 kDa cytoskeletal protein, myotilin. Its N-terminal sequence is unique, but the C-terminal half contains two Ig-like domains homologous to titin. Myotilin is expressed in skeletal and cardiac muscle, it co-localizes with [alpha]-actinin in the sarcomeric I--bands and directly interacts with [alpha]-actinin. The human myotilin gene maps to chromosome 5q31 between markers AFM350yB1 and D5S500. The locus of a dominantly inherited limb-girdle muscular dystrophy (LGMD1A) resides in an overlapping narrow segment, and a new type of distal myopathy with vocal cord and pharyngeal weakness (VCPMD) has been mapped to the same locus. The muscle specificity and apparent role as a sarcomeric structural protein raise the possibility that defects in the myotilin gene may cause muscular dystrophy.  (+info)

Postmortem proteolysis and calpain/calpastatin activity in callipyge and normal lamb biceps femoris during extended postmortem storage. (8/917)

The present experiment was conducted to determine whether calpastatin inhibits only the rate, or both the rate and extent, of calpain-induced postmortem proteolysis. Biceps femoris from normal (n = 6) and callipyge (n = 6) lamb was stored for 56 d at 4 degrees C. Calpastatin activity was higher (P < .05) in the callipyge muscle at 0 and 14 d postmortem, but not at 56 d postmortem. The activity of mu-calpain did not differ between normal and callipyge biceps femoris at 0 and 56 d postmortem (P > .05), but was higher at 14 d postmortem in the callipyge muscle (P < 0.05). The activity of m-calpain was higher in the callipyge muscle (P < 0.05). Western blot analyses of titin, nebulin, dystrophin, myosin heavy chain, vinculin, alpha-actinin, desmin, and troponin-T indicated that postmortem proteolysis was less extensive in callipyge than in normal biceps femoris at all postmortem times. The results of this experiment indicate that calpastatin inhibits both the rate and extent of postmortem proteolysis.  (+info)

购买alpha Actinin 4兔多克隆抗体(ab117411),alpha Actinin 4抗体经WB,ELISA验证,可与人,小鼠,大鼠,Monkey样本反应。中国现货速达。
alpha Actinin ELISA Kits für viele Reaktivitäten. Huhn, Rind (Kuh), Hund und weitere. alpha Actinin ELISA Kits vergleichen und bestellen.
University of Helsinki, Faculty of Biosciences, Department of Biological and Environmental Sciences, Division of genetics and Institute of Biotechnology. The actin cytoskeleton is essential for a large variety of cell biological processes. Actin exists in either a monomeric or a filamentous form, and it is very important for many cellular functions that the local balance between these two actin populations is properly regulated. A large number of proteins participate in the regulation of actin dynamics in the cell, and twinfilin, one of the proteins examined in this thesis, belongs to this category. The second level of regulation involves proteins that crosslink or bundle actin filaments, thereby providing the cell with a certain shape. α-Actinin, the second protein studied, mainly acts as an actin crosslinking protein. Both proteins are conserved in organisms ranging from yeast to mammals. In this thesis, the roles of twinfilin and α-actinin in development were examined using Drosophila ...
References for Abcams Recombinant Human Sarcomeric Alpha Actinin protein (ab92234). Please let us know if you have used this product in your publication
Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) has been cited in 63 publications. References for Human, Mouse, Rat, Goat, Pig, Xl, Xp in Flow Cyt…
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Mouse Monoclonal Anti-Alpha Actinin 4 Antibody (4D10) [DyLight 488]. Validated: WB, ELISA, ICC/IF, IHC, IHC-P. Tested Reactivity: Human, Mouse, Rat. 100% Guaranteed.
ウサギ・ポリクローナル抗体 ab59468 交差種: Ms,Hu 適用: WB,IHC-P,ICC/IF…alpha Actinin 4抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody 製品。国内在庫と品質保証制度も充実。
Ni-NTA magnetic agarose beads (Qiagen, Valencia, CA) were used to immobilize histidine-tagged proteins in binding assays performed according to the manufacturers protocol. In brief, 30 μg of histidine-tagged recombinant proteins (His-N-RAP-SR, His-N-RAP-LIM, His-N-RAP-IB, His-CAT) were incubated with 100 μl of Ni-NTA magnetic agarose beads for 1.5 hours at room temperature under denaturing conditions (8 M urea, 0.1 M NaH2PO4, 0.01 M Tris-Cl, 0.005% Tween 20, pH 8.0). The recombinant proteins immobilized on the Ni-NTA matrix were renatured by sequential incubations in 4 M, 2 M, 1 M, and 0 M urea in phosphate buffer (0.1 M NaH2PO4, 0.01 M Tris-Cl, 0.005% Tween 20, pH 8.0) over a period of 2 hours. They were then incubated with potentially interacting biomolecules (Krp1, filamin or α-actinin) at final monomer concentrations of 2.5 μg/ml (0.037 μM), 33 μg/ml (0.13 μM) and 33μg/ml (0.33 μM), respectively. Purified chicken gizzard filamin was purchased from Research Diagnostics (Flanders, ...
Actinin alpha (pan), 0.1 mg. The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins.
Since Uzgiris and Kornberg showed that lipid monolayers formed excellent substrates for 2-D crystallization of proteins and macromolecular assemblies. We are using this technique to crystallize cytoskeletal proteins and their complexes. One of these molecules currently being studied is the actin crosslinking protein alpha-actinin. 2-D crystals of alpha-actinin have been formed on positively charged lipid monolayers. In addition, paracrystalline aggregates of F-actin and the complex between F-actin and α-actinin have also been formed on these same lipid monolayers. The technology for formation of 2-D paracrystalline bundles of F-actin on lipid monolayers makes it possible to study additional complexes between F-actin and its crosslinkers which hitherto had formed 3-D gels or bundles. The image to the right was published from Biophys. J. 67, 1976-1983 in 1994.. ...
高い抗原親和性、特異性と安定した品質を兼ね備えたアブカムのウサギ・モノクローナル抗体 RabMAb® ab68167 交差種: Ms,Rat,Hu 適用: WB,IP,IHC-P
ABP-120 and non-muscle α-actinin are members of a family of F-actin cross-linkers that are grouped based on the high degree of amino acid similarity of their actin-binding domains. Although these two proteins have been extensively characterized, little is known about whether they bind to different kinds of F-actin structures and what regulates their binding. GFP fusion proteins were constructed consisting of either the entire cross-linking protein or its actin-binding domain alone. The localization of these fluorescent proteins was examined in living cells under a variety of conditions known to involve actin. The localization patterns of ABP-120 (GFP120) and α-Actinin (GFPα-A) were overlapping but distinct. GFP120 localized to the cell cortex as well as to new pseudopods and to the cortex of polarized cells, but was observed to localize more to the rear of the cell during cAMP and under agarose folate chemotaxis. GFPα-A was enriched in new pseudopods and to the F-actin filled leading edge of
ACTN4 [ENSP00000252699]. Non-muscle alpha-actinin 4; F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA; Belongs to the alpha-actinin family.. Synonyms: ACTN4, ACTN4p, hACTN4, H7C144, K7EJH8 .... Linkouts: STRING Pharos UniProt OMIM ...
Histone lysine methylation is considered to be a relatively stable modification associated with important functions in epigenetic gene control and for organizing chromatin domains. Genes encoding mammalian homologues of the Drosophila suppressor of PEV Su(var)3-9 were the first shown to encode proteins with histone lysine methyl-transferase (HKMT) activity. A hallmark signature of this class of proteins is the evolutionary conserved SET-domain found in numerous chromatin regulators, and was named for its occurrence in genes encoding three such regulators in Drosophila, namely Su(var)3-9, E(z) and trithorax. Here we describe the characterization of a putative SET-domain gene in Drosophila melanogaster, G9a. The gene encodes a protein of 1637 amino acids with similar domain architecture as the mammalian homologue of same name. Whole mount in situ hybridization shows that the gene is maternal and immunostaining shows nuclear localization of DmG9a. A yeast two-hybrid screening revealed that DmG9a ...
Principal Investigator:AKASAKA Yoshikiyo, Project Period (FY):2006 - 2007, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:Experimental pathology
The proband (II-15) was an 82-year-old man with mild, asymmetrical LV hypertrophy localized to the basal and midseptum, marked biatrial dilatation, and a restrictive LV filling pattern with preserved systolic function. He had been diagnosed with nonobstructive HCM almost 3 decades earlier and followed at our institution since 2005. Remarkably, he had a history of paroxysmal AF that presented at the age of 30, which subsequently evolved into permanent AF with advanced AV block, requiring VVI pacing at the age of 68 (Figure 2). In 2008, he proved to be negative for mutations in the coding regions and splice sites of the 8 most prevalent sarcomere genes. Despite his early onset of disease manifestations and adverse cardiac remodeling, consistent with restrictive evolution of HCM, he remained fully active with only mild functional limitation (functional class New York Heart Association class II). Furthermore, he remained free from cardioembolic complications although he repeatedly refused treatment ...
UniProtKB IDs are not shown, 1) if protein (or gene) isotypes are not specified in the referred articles, 2) if their IDs are not found in the UniprotKB database or by us ...
Deficiency of the fast-twitch muscle protein alpha-actinin-3 due to homozygosity for a nonsense polymorphism (R577X) in the ACTN3 gene is common in humans. alpha-Actinin-3 deficiency (XX) is associated with reduced muscle strength/power and enhanced endurance performance in elite athletes and in the general population. The association between R577X and loss in muscle mass and function (sarcopenia) has previously been investigated in a number of studies in elderly humans. The majority of studies report loss of ACTN3 genotype association with muscle traits in the elderly, however, there is some indication that the XX genotype may be associated with faster muscle function decline. To further explore these potential age-related effects and the underlying mechanisms, we examined the effect of alpha-actinin-3 deficiency in aging male and female Actn3 knockout (KO) mice (2, 6, 12, and 18 months). Our findings support previous reports of a diminished influence of ACTN3 genotype on muscle performance in the
Alpha-actinin (alpha-actinin-2) is a protein which links the NR1 and NR2B subunits of N-methyl-D-aspartate (NMDA) glutamate receptors to the actin cytoskeleton. Because of the importance of NMDA receptors in modulating the function of the striatum, we have examined the localization of alpha-actinin-2 protein and mRNA in striatal neurons, and its biochemical interaction with NMDA receptor subunits present in the rat striatum. Using an alpha-actinin-2-specific antibody, we found intense immunoreactivity in the striatal neuropil and within striatal neurons that also expressed parvalbumin, calretinin and calbindin. Conversely, alpha-actinin-2 immunoreactivity was not detected in neurons expressing choline acetyltransferase and neuronal nitric oxide synthase. Dual-label in situ hybridization revealed that the highest expression of alpha-actinin-2 mRNA is in substance P-containing striatal projection neurons. The alpha-actinin-2 mRNA is also present in enkephalinergic projection neurons and ...
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Dictyostelium alpha-actinin is a Ca(2+)-regulated F-actin cross-linking protein. To test the inhibitory function of the two EF hands, point mutations were introduced into either one or both Ca(2+)-binding sites. After mutations, the two EF hands were distinguishable with respect to their regulatory activities. Inactivation of EF hand I abolished completely the F-actin cross-linking activity of Dictyostelium discoideum alpha-actinin but Ca2+ binding by EF hand II was still observed in a 45Ca2+ overlay assay. In contrast, after mutation of EF hand II the molecule was still active and inhibited by Ca2+; however, approximately 500-fold more Ca2+ was necessary for inhibition and 45Ca2+ binding could not be detected in the overlay assay. These data indicate that EF hand I has a low affinity for Ca2+ and EF hand II a high affinity, implying a regulatory function of EF hand I in the inhibition of F-actin cross-linking activity. Biochemical data is presented which allows us to distinguish two functions ...
Clearly my myosin LC2 and my alpha actinin genes count as two (or is it 4, because I have two copies of each), but do we have 2 more if we add in those of the mouse? And how about the chimpanzee, if we discover that (unlike the mouse) their genes/alleles are absolutely identical to mine? Do we have 2 more when we add in the alleles of the mayor of my town (which, unbeknowst to her have synonomous substitions that make the protein-encoding portion of her DNA, but not the protein itself, different from mine)? Or how about those of the guy next door, who has a neutral mutation in an intron region? Or my third cousin, who has a neutral AA substition in his proteins ...
Recombinant protein from the full-length sequence of homo sapiens actinin alpha 1 (ACTN1), transcript variant 2 (NM_001102), with a His tag., from EUPROTEIN
The muscle protein, which covers the active sites on the actin filament at rest, is: a. Troponin-I b. Troponin-C c. Tropomyosin d. Myosin e. Actinin
Our previous series of studies have shown that l-afadin is essential for the colocalization and compact clustering of nectin with E-cadherin at cell-cell AJs and proper organization of E-cadherin-based cell-cell AJs (Ikeda et al. 1999; Takahashi et al. 1999; Miyahara et al. 2000). Extending these earlier observations, we have shown here that the COOH-terminal half of α-catenin is, furthermore, essential for this colocalization. This colocalization is not mediated through the cytoplasmic region of E-cadherin or β-catenin. α-Catenin directly interacts with vinculin and α-actinin through the NH2-terminal half (Knudsen et al. 1995; Nieset et al. 1997; Watabe-Uchida et al. 1998; Imamura et al. 1999) and with ZO-1 through the COOH-terminal half (Itoh et al. 1997; Imamura et al. 1999). All of these α-catenin-binding proteins directly interact with F-actin (Burridge and Feramisco 1982; Menkel et al. 1994; Johnson and Craig 1995; Itoh et al. 1997; Fanning et al. 1998). The present results show that ...
To examine the function of CRP2 in vivo, we generated Csrp2−/− mice by targeted mutation. The LIM domains of CRP2 mediate interactions with its binding partners including zyxin, α-actinin, and CRP2BP.12,19,30 Thus, we targeted the first LIM domain by disrupting exon 3, the largest coding exon. No message was detected in Csrp2−/− mouse RNA when exon 3 was used as a probe, although a smaller transcript was detected by RT-PCR. Nevertheless, no CRP2 was detected in protein isolated from Csrp2−/− mouse aorta by Western blot analysis using CRP2(91-98) antiserum. Furthermore, immunostaining of aortic sections with CRP2(93-108) antiserum did not detect CRP2 expression in Csrp2−/− mice. We cannot exclude the possibility that a truncated protein could be generated because the two antisera used in this study were against epitopes C-terminal to those encoded by exon 2. However, even if the truncated transcript were translated, it would not encode either LIM domain due to a frame shift. ...
apical plasma membrane, cell-cell adherens junction, focal adhesion, actinin binding, protein C-terminus binding, ubiquitin-protein transferase activity, cell-cell adhesion
This paper provides a comprehensive explanation of striated muscle mechanics and contraction on the basis of filament rotations. Helical proteins, particularly the coiled-coils of tropomyosin, myosin and α-actinin, shorten their H-bonds cooperatively and produce torque and filament rotations when the Coulombic net-charge repulsion of their highly charged side-chains is diminished by interaction with ions. The classical
Heterogeneity of SMC is a well-known phenomenon. The interpretation, however, of the morphologic phenotype as well as the functional implications of the expression of differentiation markers is subject to much debate. The data are derived from in vivo or in vitro studies. The material used varies from adult to neonatal to fetal vessels and cells from different animal species. The origin of the cultured cells can be from intima, inner or outer media, or the adventitia in both normal and diseased vessels.. The data from in vitro studies are largely reflected in in vivo studies. Proceeding from data in early fetal development, it has been shown that the SMC start to express SM α-actin17 followed by 1E12,20 an actinin marker, and smoothelin.21 Relative late differentiation markers are the already mentioned markers, such as SM22, calponin, h-caldesmon,7 and SM myosin.8 These are upregulated until the expression level of the mature vessel wall is reached.9 11 During this developmental period, there ...
Immunocytochemistry for confocal microscopy. Sections were blocked in 20% normal donkey serum (NDS; Jackson ImmunoResearch, West Grove, PA) in 0.05 m PBS, pH 7.4, then incubated in various combinations of primary antibodies for cortactin, α-actinin, synaptophysin, and VGLUT1 in PBS containing 2% NDS overnight at room temperature. After several washes, sections were incubated in secondary antibodies (anti-rabbit Cy3 for cortactin, anti-mouse FITC for synaptophysin and VGLUT1). Alexa Fluor-488 conjugated to phalloidin (Molecular Probes, Eugene, OR) was used for visualization of F-actin (Allison et al., 1998). For visualization of cell processes, we used the lipophilic dye 3,3′-dioctadecyloxacarbocyanidine perchlorate (DiO; Molecular Probes), which infiltrates the plasma membrane, labeling even the finest neuronal processes (for details, see Burette et al., 2002). After several washes, sections were mounted on glass slides, coverslipped in Vectashield (Vector Laboratories, Burlingame, CA) and ...
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (By similarity).
Loss of CH-ILKBP impairs the membrane translocation of PKB/Akt. (A-E) The CH-ILKBP siRNA-transfectants (lanes 1 and 2), the control transfectants (lanes 3 and
Things will be a lot different when we are older. Perhaps when your grandchild is born, right after they snip the umbilical cord, they will sequence their genome. Immediately, some computer will pick out genetic markers for specific disease states, and maybe a doctor (possibly some gene-ologist of sorts) will do a little bit of gene therapy to prevent the more serious diseases from developing. Perhaps for those really wealthy parents, a few more options may open for tweaking. If you dont like the hair colour your childs genes code for, just check the appropriate box and they can take care of that. What about sports? Would you prefer a power athlete, maybe a sprinter, or would you rather your child be tailored for endurance? For that option, perhaps they would look at tinkering with the ACTN3 gene, which encodes the muscle fiber protein alpha-actinin-3. As it turns out, you could probably already predict genetic predisposition of muscles for either power or endurance. There is evidence for certain
Mutations in 1-actinin-4 (Actn4), an actin crosslinking protein encoded by the human ACTN4 gene, cause an autosomal dominant form of focal segmental glomerulosc...
Complete information for ACTN1-AS1 gene (RNA Gene), ACTN1 Antisense RNA 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
The topographical relationship between stress fiber-like structures (SFLS) and nascent myofibrils was examined in cultured chick cardiac myocytes by immunofluorescence microscopy. Antibodies against muscle-specific light meromyosin (anti-LMM) and desmin were used to distinguish cardiac myocytes from fibroblastic cells. By various combinations of staining with rhodamine-labeled phalloidin, anti-LMM, and antibodies against chick brain myosin and smooth muscle alpha-actinin, we observed the following relationships between transitory SFLS and nascent and mature myofibrils: (a) more SFLS were present in immature than mature myocytes; (b) in immature myocytes a single fluorescent fiber would stain as a SFLS distally and as a striated myofibril proximally, towards the center of the cell; (c) in regions of a myocyte not yet penetrated by the elongating myofibrils, SFLS were abundant; and (d) in regions of a myocyte with numerous mature myofibrils, SFLS had totally disappeared. Spontaneously contracting ...
Skeletal muscle actin binding protein spin-down assay kit provides G- or F-actin plus positive (α-actinin ) and negative (Bovine Serum Albumin, BSA) binding control proteins. Kit contains skeletal muscle actin.
TY - JOUR. T1 - C3a receptor blockade protects podocytes from injury in diabetic nephropathy. AU - Morigi, Marina. AU - Perico, Luca. AU - Corna, Daniela. AU - Locatelli, Monica. AU - Cassis, Paola. AU - Carminati, Claudia Elisa. AU - Bolognini, Silvia. AU - Zoja, Carlamaria. AU - Remuzzi, Giuseppe. AU - Benigni, Ariela. AU - Buelli, Simona. PY - 2020/3/12. Y1 - 2020/3/12. N2 - Renal activation of the complement system has been described in patients with diabetic nephropathy (DN), although its pathological relevance is still ill-defined. Here, we studied whether glomerular C3a, generated by uncontrolled complement activation, promotes podocyte damage, leading to proteinuria and renal injury in mice with type 2 diabetes. BTBR ob/ob mice exhibited podocyte loss, albuminuria, and glomerular injury accompanied by C3 deposits and increased C3a and C3a receptor (C3aR) levels. Decreased glomerular nephrin and α-actinin4 expression, coupled with integrin-linked kinase induction, were also observed. ...
de Almeida Ribeiro E., Pinotsis N., Ghisleni A., Salmazo A., Konarev P. V., Kostan J., Sjöblom B., Schreiner C., Polyansky A., Gkougkoulia E. A., Holt M. R., Aachmann F. L., Žagrović B., Bordignon E., Pirker K. F., Svergun D. I., Gautel M., Djinović-Carugo K. (2014), The structure of human muscle α-actinin: Insight into the intramolecular regulation of ligand binding and Z-disk assembly, Cell 159(6):1447-60. ...
de Almeida Ribeiro E., Pinotsis N., Ghisleni A., Salmazo A., Konarev P. V., Kostan J., Sjöblom B., Schreiner C., Polyansky A., Gkougkoulia E. A., Holt M. R., Aachmann F. L., Žagrović B., Bordignon E., Pirker K. F., Svergun D. I., Gautel M., Djinović-Carugo K. (2014), The structure of human muscle α-actinin: Insight into the intramolecular regulation of ligand binding and Z-disk assembly, Cell 159(6):1447-60. ...
Many eukaryotes have long slender motile cytoplasmic projections, called flagella. These are composed mainly of tubulin and shorter cilia, both of which are variously involved in movement, feeding, and sensation. These are entirely distinct from prokaryotic flagella. They are supported by a bundle of microtubules arising from a basal body, also called a kinetosome or centriole, characteristically arranged as nine doublets surrounding two singlets. Flagella also may have hairs, or mastigonemes, and scales connecting membranes and internal rods. Their interior is continuous with the cells cytoplasm. Microfilamental structures composed by actin and actin binding proteins, e.g., α-actinin, fimbrin, filamin are present in submembraneous cortical layers and bundles, as well. Motor proteins of microtubules, e.g., dynein or kinesin and actin, e.g., myosins provide dynamic character of the network. Centrioles are often present even in cells and groups that do not have flagella. They generally occur in ...
Clone REA199 recognizes CD144 (VE-Cadherin), a 120 KDa type II cadherin. Cadherins are cell adhesion molecules and mediate Ca2+ dependent homophilic interactions. CD144 contains five extracellular cadherin (EC) domains and like other cadherins can interact directly via its C-terminus with cytoplasmic proteins such as β-catenin, plaktoglobin, and p120. Plaktoglobin und β-catenin bind to α-catenin, which in turn interacts with several actin-binding proteins, α-actinin, ajuba, zonula occludens-1 (ZO-1). Further indirect interactions of CD144 with partners such as SHP-2, VEGFR-2, Csk, and PAR-3, 6 allows CD144 to not only regulate the stability and strength of cell adhesion but also to serve functions such as sensing of shear forces, anti-proliferative, and anti-apoptotic effects. Additional information: Clone REA199 displays negligible binding to Fc receptors. - Ireland
Clone REA199 recognizes CD144 (VE-Cadherin), a 120 KDa type II cadherin. Cadherins are cell adhesion molecules and mediate Ca2+ dependent homophilic interactions. CD144 contains five extracellular cadherin (EC) domains and like other cadherins can interact directly via its C-terminus with cytoplasmic proteins such as β-catenin, plaktoglobin, and p120. Plaktoglobin und β-catenin bind to α-catenin, which in turn interacts with several actin-binding proteins, α-actinin, ajuba, zonula occludens-1 (ZO-1). Further indirect interactions of CD144 with partners such as SHP-2, VEGFR-2, Csk, and PAR-3, 6 allows CD144 to not only regulate the stability and strength of cell adhesion but also to serve functions such as sensing of shear forces, anti-proliferative, and anti-apoptotic effects. Additional information: Clone REA199 displays negligible binding to Fc receptors. - Schweiz
Myosin II is a motor protein found in the cytoskeleton in the cytoplasm of cells. It is responsible for enpowering actin to contract. In [[A quantitative analysis of contractility in active cytoskeletal protein networks]] by Weitz et al. Show the role of myosin in actin contraction. It was shown that without appropriate amounts of ,math>alpha,/math>-actinin, myosin cannot produce contraction. ==References== http://en.wikipedia.org/wiki/Myosin ...
Walikonis, Randall S. and Oguni, Asako and Khorosheva, Eugenia M. et al. (2001) Densin-180 forms a ternary complex with the α-subunit of Ca^(2+)/calmodulin-dependent protein kinase II and α-actinin. Journal of Neuroscience, 21 (2). pp. 423-433. ISSN 0270-6474. PMCID PMC6763799. https://resolver.caltech.edu/CaltechAUTHORS:20120424-150007753 ...
Methods and Results: Distribution of Islet-1+ cells in adult heart was investigated using transgenic mice with nuclear β-galactosidase inserted into the Islet-1 locus. nLacZ-positive cells were only present in 3 regions of the adult heart: clusters in the interatrial septum, scattered within the wall of the great vessels, and a strictly delimited cluster between right atrium and superior vena cava. Islet-1+ cells in the first type of clusters coexpressed markers for parasympathetic neurons. Positive cells in the great arteries coexpressed smooth muscle actin and β-myosin heavy chain, indicating a smooth muscle cell identity. Very few Islet-1+ cells within the outflow tract expressed the cardiomyocyte marker α-actinin. Islet-1+ cells in the right atrium coexpressed the sinoatrial node pacemaker cell marker HCN4. Cell number and localization remained unchanged between 1 to 18 months of age. Consistently Islet-1 mRNA was detected in human sinoatrial node. Islet-1+ cells could not be detected in ...
test were utilized. through ubiquitination. Over-expression of IQGAP1 in charge MEF phenocopied the migration and growing flaws of cells. On the other hand, siRNA-mediated knockdown of IQGAP1 rescued the flaws in cellular motion of cells. Conclusions The E3 ligase activity of Hectd1 regulates the protein degree of IQGAP1 through ubiquitination and for that reason mediates the dynamics of FXs like the recruitment of paxillin and actinin. IQGAP1 is among the effectors of HECTD1. Electronic supplementary materials The online edition of this content (doi:10.1186/s12964-016-0156-8) contains supplementary materials, which is open to authorized users. mice elevated the cranial mesenchyme cell migration [16, 17] however the results from Li and coworkers demonstrated that knockdown of HECTD1 inhibits TGFBR2 the migration of breasts cancer tumor MDA-MB-231 cells [18]. To solve this contradictory concern, we have utilized the Hectd1 homozygous mutant (mutation mice [33], the gene-trap mouse embryonic stem ...
Identification, characterization, and synthesis of peptide epitopes and a recombinant six-epitope protein for Trichomonas vaginalis serodiagnosis J F Alderete, Calvin J NeaceSchool of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USAAbstract: There is a need for a rapid, accurate serodiagnostic test useful for both women and men infected by Trichomonas vaginalis, which causes the number one sexually transmitted infection (STI). Women and men exposed to T. vaginalis make serum antibody to fructose-1,6-bisphosphate aldolase (ALD), α-enolase (ENO), and glyceraldehyde-3-phosphate dehydrogenase (GAP). We identified, by epitope mapping, the common and distinct epitopes of each protein detected by the sera of women patients with trichomonosis and by the sera of men highly seropositive to the immunogenic protein α-actinin (positive control sera). We analyzed the amino acid sequences to determine the extent of identity of the epitopes of each protein
Connecting devices and elongated members for orthopedic medical use are disclosed. In certain embodiments, a connecting device may include a central portion that can accommodate part of an elongated member and wings for connecting to anchor members. Such central portions can be open or closed, and such wings can be solid, e.g. rod-type structures, or can be slotted. A T-shaped elongated member is also disclosed.
Use Bio-Rads PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
This gene encodes a member of the IQGAP family. The encoded protein contains three IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. This protein interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. It also acts as a tumor suppressor and has been found to play a role in regulating innate antiviral responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2017 ...
... has been shown to interact with: AKAP9, Actinin, alpha 1, CCDC85B, NEFL, NEUROD2 Phosphoinositide-dependent ... "Interaction of PKN with alpha-actinin". J. Biol. Chem. 272 (8): 4740-6. doi:10.1074/jbc.272.8.4740. PMID 9030526. Matsuzawa K, ... "Pathological shear stress stimulates the tyrosine phosphorylation of alpha-actinin associated with the glycoprotein Ib-IX ...
... alpha-actinin. Xin, and XIRP2. Nebulette was identified in 1995 by Moncman and Wang using primary cultures of chicken embryonic ...
"An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment". J. Biol ... "An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment". J. Biol ... Li B, Trueb B (2001). "Analysis of the alpha-actinin/zyxin interaction". J. Biol. Chem. 276 (36): 33328-35. doi:10.1074/jbc. ... Li B, Trueb B (September 2001). "Analysis of the alpha-actinin/zyxin interaction". J. Biol. Chem. 276 (36): 33328-35. doi: ...
CapZ interacts with α-actinin, nebulette, nebulin, HSC70. at the Z-disc. CAPZB is a member of the F-actin capping protein ... Papa I, Astier C, Kwiatek O, Raynaud F, Bonnal C, Lebart MC, Roustan C, Benyamin Y (February 1999). "Alpha actinin-CapZ, an ...
Maruyama K, Kurokawa H, Oosawa M, Shimaoka S, Yamamoto H, Ito M, Maruyama K (May 1990). "Beta-actinin is equivalent to Cap Z ...
... has been shown to interact with Keratin 18, Actinin alpha 4, Dystonin, Actinin, alpha 1, CTNND1 ... Gonzalez, A M; Otey C; Edlund M; Jones J C (December 2001). "Interactions of a hemidesmosome component and actinin family ...
They decided to compile their work on α-actinin and showed that α-actinin is distributed periodically along stress fibers. They ... Since these regions would several years later be named focal adhesions, α-actinin was the first protein found to be ... While developing a procedure to purify α-actinin from smooth muscle, Burridge co-purified another protein, vinculin, ... Lazarides E, Burridge K; Burridge (November 1975). "Alpha-actinin: immunofluorescent localization of a muscle structural ...
Liu Y, Belkina NV, Shaw S (2009). "HIV infection of T cells: actin-in and actin-out". Science Signaling. 2 (66): pe23. doi: ... For example, if the α-actinin or gelation factor gene has been removed in Dictyostelium individuals do not show an anomalous ... In contractile bundles, the actin-bundling protein alpha-actinin separates each thin filament by ≈35 nm. This increase in ... ISBN 978-84-8174-866-6. Fujiwara K, Porter ME, Pollard TD (Oct 1978). "Alpha-actinin localization in the cleavage furrow during ...
TRPP3 is abundant in mouse brain where it associates with alpha-actinin-2. Alpha-actinin attaches TRPP3 to the cytoskeleton and ... Li Q, Dai XQ, Shen PY, Wu Y, Long W, Chen CX, Hussain Z, Wang S, Chen XZ (December 2007). "Direct binding of alpha-actinin ... Li Q, Dai XQ, Shen PY, Wu Y, Long W, Chen CX, Hussain Z, Wang S, Chen XZ (December 2007). "Direct binding of alpha-actinin ... Alpha-actinin is an actin-bundling protein known to regulate several types of ion channels. Planer lipid bilayer ...
Li B, Zhuang L, Reinhard M, Trueb B (2003). "The lipoma preferred partner LPP interacts with alpha-actinin". J. Cell Sci. 116 ( ...
... has been shown to interact with Actinin alpha 4. GRCh38: Ensembl release 89: ENSG00000058404 - Ensembl, May 2017 GRCm38 ... actinin". J. Neurosci. 21 (2): 423-33. doi:10.1523/JNEUROSCI.21-02-00423.2001. PMC 6763799. PMID 11160423. Liao GY, Wagner DA, ... actinin". J. Neurosci. 21 (2): 423-33. doi:10.1523/JNEUROSCI.21-02-00423.2001. PMC 6763799. PMID 11160423. Yamamoto H (2002 ...
... has been shown to interact with Actinin, alpha 1. GRCh38: Ensembl release 89: ENSG00000171450 - Ensembl, May 2017 GRCm38 ... 5 activators p35 and p39 interact with the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II and alpha-actinin-1 in ... 5 activators p35 and p39 interact with the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II and alpha-actinin-1 in ...
Lan S, Wang H, Jiang H, Mao H, Liu X, Zhang X, Hu Y, Xiang L, Yuan Z (2003). "Direct interaction between α-actinin and ...
Yan Q, Sun W, Kujala P, Lotfi Y, Vida TA, Bean AJ (May 2005). "CART: an Hrs/actinin-4/BERP/myosin V protein complex required ... The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin ... TRIM3 has been shown to interact with Actinin alpha 4. GRCh38: Ensembl release 89: ENSG00000110171 - Ensembl, May 2017 GRCm38: ... binds to alpha-actinin-4". Biochemical and Biophysical Research Communications. 267 (3): 906-11. doi:10.1006/bbrc.1999.2045. ...
2003). "Direct interaction between alpha-actinin and hepatitis C virus NS5B". FEBS Lett. 554 (3): 289-94. doi:10.1016/S0014- ...
... with alpha-actinin 2". J Cell Biochem. 78 (4): 558-65. doi:10.1002/1097-4644(20000915)78:4. 3.0.CO;2-I. PMID 10861853. "Entrez ...
Namely, α-Actinin results in heightened pH sensitivity and desensitization recovery. They can also increase current flow ...
Wyszynski M, Lin J, Rao A, Nigh E, Beggs AH, Craig AM, Sheng M (January 1997). "Competitive binding of alpha-actinin and ... Actinin, alpha 2, DLG2, DLG3, DLG4, EXOC4, LIN7B, and RICS. NMDA receptor Glutamate receptor GRCh38: Ensembl release 89: ...
Immunostains for myotilin and α-actinin all but clinch the diagnosis. However, nemaline rods may still be visible post-mortem ...
Pardo, JV, Craig, SW (1979). "alpha-Actinin localization in the junctional complex of intestinal epithelial cells". J Cell Biol ...
2005). "Involvement of palladin and alpha-actinin in targeting of the Abl/Arg kinase adaptor ArgBP2 to the actin cytoskeleton ... 2004). "Molecular analysis of the interaction between palladin and alpha-actinin". FEBS Lett. 566 (1-3): 30-4. doi:10.1016/j. ...
Vallenius T, Luukko K, Mäkelä TP (2000). "CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4 ... PDLIM1 has been shown to interact with: Actinin alpha 4, Actinin, alpha 1, Estrogen receptor alpha, and RNF12. GRCh38: Ensembl ... "CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4". J. Biol. Chem. 275 (15): 11100-5. doi: ... with alpha-actinin 2". J Cell Biochem. 78 (4): 558-65. doi:10.1002/1097-4644(20000915)78:4. 3.0.CO;2-I. PMID 10861853. "Entrez ...
The antiparallel arrangement of actin filaments within stress fibers is reinforced by α-actinin, an actin filament crosslinking ... Lazarides, Elias; Burridge, Keith (November 1975). "α-Actinin: Immunofluorescent localization of a muscle structural protein in ... such as α-actinin, to form a highly regulated actomyosin structure within non-muscle cells. Stress fibers have been shown to ... fibers in motile and non-motile cells are similar in that they both contain actin filaments which are cross-linked by α-actinin ...
There are no RNA binding motifs or actinin type actin binding motifs. There are no N-myristoylation pattern or prenylation ...
Harper BD, Beckerle MC, Pomiès P (2001). "Fine mapping of the alpha-actinin binding site within cysteine-rich protein". Biochem ...
Maruoka ND, Steele DF, Au BP, Dan P, Zhang X, Moore ED, Fedida D (May 2000). "alpha-actinin-2 couples to cardiac Kv1.5 channels ... Maruoka ND, Steele DF, Au BP, Dan P, Zhang X, Moore ED, Fedida D (May 2000). "alpha-actinin-2 couples to cardiac Kv1.5 channels ... KCNA5 has been shown to interact with DLG4 and Actinin, alpha 2. Voltage-gated potassium channel GRCh38: Ensembl release 89: ... "A discrete amino terminal domain of Kv1.5 and Kv1.4 potassium channels interacts with the spectrin repeats of alpha-actinin-2 ...
Within the brain, dendrin interacts with α-actinin in postsynaptic dendritic spines. Together MAGI/S-SCAM, α-actinin, and ... Along with two other proteins, MAGI/S-SCAM and α-actinin, dendrin is linked to synaptic plasticity and memory formation in the ... The protein trio of MAGI/S-SCAM, α-actinin, and dendrin helps to connect postsynaptic density (PSD) to the cytoskeleton of the ...
These include spectrin, alpha-actinin, dystrophin and more recently the plakin family. The spectrin repeat forms a three-helix ...
Olski TM, Noegel AA, Korenbaum E (Feb 2001). "Parvin, a 42 kDa focal adhesion protein, related to the alpha-actinin superfamily ... 2004). "Affixin interacts with alpha-actinin and mediates integrin signaling for reorganization of F-actin induced by initial ...
Olski TM, Noegel AA, Korenbaum E (Feb 2001). "Parvin, a 42 kDa focal adhesion protein, related to the alpha-actinin superfamily ...
Actinin is a microfilament protein. α-Actinin is necessary for the attachment of actin filaments to the Z-lines in skeletal ... The non-sarcomeric α-actinins, encoded by ACTN1 and ACTN4, are widely expressed. ACTN2 expression is found in both cardiac and ... Both ends of the rod-shaped α-actinin dimer contain actin-binding domains. Mutations in ACTN4 can cause the kidney disease ... ISBN 978-0-08-092427-4. Actinin at the US National Library of Medicine Medical Subject Headings (MeSH) v t e. ...
Alpha-actinin-3, also known as alpha-actinin skeletal muscle isoform 3 or F-actin cross-linking protein, is a protein that in ... ACTN3 has been shown to interact with Actinin, alpha 2. Actinin GRCh38: Ensembl release 89: ENSG00000248746 - Ensembl, May 2017 ... "Entrez Gene: ACTN3 actinin, alpha 3". Beggs AH, Byers TJ, Knoll JH, Boyce FM, Bruns GA, Kunkel LM (May 1992). "Cloning and ... Alpha-actinin is an actin-binding protein with multiple roles in different cell types. This gene expression is limited to ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
The human sarcomeric alpha-actinins (ACTN2 and ACTN3) are major structural components of the Z line in skeletal muscle; they ... alpha-actinin-3 and performance Med Sport Sci. 2009;54:88-101. doi: 10.1159/000235698. Epub 2009 Aug 17. ... In turn, the shift towards a slow muscle phenotype in fast muscle fibers likely explains why loss of alpha-actinin-3 is ... Homozygosity for the R577X null-allele results in the absence of alpha-actinin-3 in fast muscle fibers with frequencies that ...
alpha-actinin-1. Names. F-actin cross-linking protein. alpha actinin 1a. alpha-actinin cytoskeletal isoform. non-muscle alpha- ... Alpha-actinin interaction with syndecan-4 has a role in stabilising cell-matrix adhesion. Title: Alpha-actinin interactions ... Actn1 actinin, alpha 1 [Mus musculus] Actn1 actinin, alpha 1 [Mus musculus]. Gene ID:109711 ... actinin, alpha 1provided by MGI. Primary source. MGI:MGI:2137706 See related. Ensembl:ENSMUSG00000015143 Gene type. protein ...
The actinin family of actin cross-linking proteins - a genetic perspective Actinins are one of the major actin cross-linking ... The role of alpha-actinin-4 in human kidney disease Mutations in the Alpha-actinin-4 gene (ACTN4) cause a rare form of familial ... The actinin family proteins: biological function and clinical implications. Guest edited by Dr Hung-Ying Kao ... The biological role of actinin-4 (ACTN4) in malignant phenotypes of cancer Invasion and metastasis are malignant phenotypes in ...
125I-alpha-actinin was capable of interacting with vinculin and metavinculin. The specific binding of 125-I-alpha-actinin to ... We suggest that the ability of vinculin and alpha-actinin to form a complex may be realized in microfilament-membrane linkages. ... Using the gel overlay technique, we detected strong binding of 125I-vinculin and 125I-metavinculin to alpha-actinin, 175 kDa ... Interaction of iodinated vinculin, metavinculin and alpha-actinin with cytoskeletal proteins.. Belkin AM, Koteliansky VE. ...
Further, TRPP3 was abundantly present in mouse brain where it associates with alpha-actinin-2. Taken together, alpha-actinin ... Direct binding of alpha-actinin enhances TRPP3 channel activity J Neurochem. 2007 Dec;103(6):2391-400. doi: 10.1111/j.1471- ... The TRPP3-alpha-actinin association was documented by co-immunoprecipitation using native cells and tissues, yeast two-hybrid, ... It remains to be determined whether the TRPP3-alpha-actinin interaction is relevant to acid sensing and other functions in ...
Anti-Sarcomeric Alpha Actinin antibody (ab72592) has been cited in 20 publications. References for Human, Mouse, Rat in IF, IHC ...
Compare alpha-actinin-2 ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and ... alpha-actinin-2 ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established antibody-based tool for ... Your search returned 110 alpha-actinin-2 ELISA ELISA Kit across 14 suppliers. ...
PS00019, ACTININ_1, 1 hit. PS00020, ACTININ_2, 1 hit. PS50021, CH, 2 hits. PS50222, EF_HAND_2, 2 hits. ... PS00019, ACTININ_1, 1 hit. PS00020, ACTININ_2, 1 hit. PS50021, CH, 2 hits. PS50222, EF_HAND_2, 2 hits. ... "Myozenin: an alpha-actinin- and gamma-filamin-binding protein of skeletal muscle Z lines.". Takada F., Vander Woude D.L., Tong ... IPR001589, Actinin_actin-bd_CS. IPR001715, CH-domain. IPR036872, CH_dom_sf. IPR011992, EF-hand-dom_pair. IPR014837, EF- ...
PS00019. ACTININ_1. 1 hit. PS00020. ACTININ_2. 1 hit. PS50021. CH. 2 hits. PS50222. EF_HAND_2. 2 hits. ... PS00019. ACTININ_1. 1 hit. PS00020. ACTININ_2. 1 hit. PS50021. CH. 2 hits. PS50222. EF_HAND_2. 2 hits. ... IPR001589. Actinin_actin-bd_CS. IPR036872. Calponin-like_dom_sf. IPR001715. CH-domain. IPR011992. EF-hand-dom_pair. IPR014837. ... IPR001589. Actinin_actin-bd_CS. IPR036872. Calponin-like_dom_sf. IPR001715. CH-domain. IPR011992. EF-hand-dom_pair. IPR014837. ...
References for Abcams Recombinant Human Sarcomeric Alpha Actinin protein (ab92234). Please let us know if you have used this ...
Compare Anti-Alpha-Actinin-4 Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, ...
Browse our Alpha Actinin 2 Protein catalog backed by our Guarantee+. ... Alternate Names for Alpha Actinin 2 Proteins. Alpha Actinin 2 protein, ACTN2 protein, actinin, alpha 2 protein, Alpha-actinin ... Alpha Actinin 2 Proteins. We offer Alpha Actinin 2 Peptides and Alpha Actinin 2 Proteins for use in common research ... Our Alpha Actinin 2 Peptides and Alpha Actinin 2 Proteins can be used in a variety of model species: Human. Use the list below ...
... alpha-actinin explanation free. What is alpha-actinin? Meaning of alpha-actinin medical term. What does alpha-actinin mean? ... Looking for online definition of alpha-actinin in the Medical Dictionary? ... alpha-actinin. α-ac·tin·in. (ak-tinin), An F-actin binding protein in vertebrate cells that cross-links actin filaments into ... Actin-binding protein alpha-actinin 4 (ACTN4) is a transcriptional co-activator of RelA/p65 sub-unit of NF-kB.. The Short ...
1997). "Interaction of PKN with alpha-actinin". J. Biol. Chem. 272 (8): 4740-6. PMID 9030526.. CS1 maint: Explicit use of et al ... 2001). "FATZ, a filamin-, actinin-, and telethonin-binding protein of the Z-disc of skeletal muscle". J. Biol. Chem. 275 (52): ... 2000). "Interaction of hCLIM1, an enigma family protein, with alpha-actinin 2". J. Cell. Biochem. 78 (4): 558-65. PMID 10861853 ... Actinin, alpha 2, also known as ACTN2, is a human gene.[1] ... Alpha actinin is an actin-binding protein with multiple roles ...
Among several others, we found 52 cDNA clones encoding α-actinin-4 and 6 clones encoding α-actinin-1. α-Actinin-4-mediated FSGS ... Full-length α-actinin-4 was amplified by PCR from pGFP-α-actinin-4 and inserted into pFLAG-CMV-5a vector. All constructs were ... Anti-α-actinin (sarcomeric; Sigma-Aldrich) and anti-α-actinin-4 (11) were used at 1:4,000 and 1:5,000, respectively. ... Synpo-long and Synpo-short interact with α-actinin-2 and α-actinin-4. In an effort to delineate isoform-specific differences in ...
What is actinin alpha4 isoform? Meaning of actinin alpha4 isoform medical term. What does actinin alpha4 isoform mean? ... Looking for online definition of actinin alpha4 isoform in the Medical Dictionary? actinin alpha4 isoform explanation free. ... redirected from actinin alpha4 isoform) ACTN4. A gene on chromosome 14q24.1 that encodes an actin-binding bundling protein ... The non-muscle, alpha-actinin isoform of ACTN4 is concentrated in the cytoplasm, and may be involved in metastasis; ACTN4 ...
Invitrogen Anti-alpha Actinin 4 Polyclonal, Catalog # PA5-22259. Tested in Western Blot (WB), Immunofluorescence (IF), ... Protein Aliases: actinin-Alpha-4; alpha actinin 4; Alpha-actinin-4; F-actin cross-linking protein; focal segmental ... Cite alpha Actinin 4 Polyclonal Antibody. The following antibody was used in this experiment: alpha Actinin 4 Polyclonal ... alpha Actinin 4 Polyclonal Antibody. View all (14) alpha Actinin 4 antibodies ...
For an estimate of lifetimes, it is important to note that α-actinin in muscle is also bound to actin, keeping each α-actinin ... Mechanical Properties of the Interaction Between the α-Actinin EF3-4 and the α-Actinin Neck Region (Fig. S8).. To study the ... 22) assessed affinities of all repeats to α-actinin and found measurable affinities to α-actinin 2 for T1, T3, and T7. ... that every other Z-repeat can bind α-actinin in the Z-disk. Hence, if seven repeats are present, up to four α-actinins will ...
... a-actinin complexes, which will be followed by future experiments probing the interactions between F-actin/a-actinin and CL-DNA ... Confocal images of Factin complexed with a-actinin in vitro (a-actinin:G-actin=1:25) reveals the formation of a network of ... 2, left), but where the F-actin - F-actin correlations (observed for g ≤ 10) have not yet set in due to the low a-actinin ... When filamentous actin is allowed to mix in-vitro with a-actinin, an actin cross-linking protein purified from cells, a ...
alpha Actinin ELISA Kits für viele Reaktivitäten. Huhn, Rind (Kuh), Hund und weitere. alpha Actinin ELISA Kits vergleichen und ... alpha-actinin-2 , alpha-actinin-3 , alpha-actinin , alpha-actinin-4 , non-muscle alpha-actinin 4 ... alpha Actinin (ACTN) ELISA Kits. Bezeichnung:. alpha-Actinin Skeletal Muscle ELISA Kits (ACTN). Auf www.antikoerper-online.de ... the interaction between GNE (zeige GNE ELISA Kits) and alpha-actinin 1 (zeige ACTN1 ELISA Kits) and alpha-actinin 2 (zeige ...
Structural Dynamics of α-Actinin-Vinculin Interactions. Philippe R. J. Bois, Robert A. Borgon, Clemens Vonrhein, Tina Izard ... Structural Dynamics of α-Actinin-Vinculin Interactions Message Subject (Your Name) has forwarded a page to you from Molecular ...
Alpha-actinin-4, F-actin cross-linking protein, Non-muscle alpha-actinin 4, ACTN4 ...
α-Actinin TvACTN3 of Trichomonas vaginalis Is an RNA-Binding Protein That Could Participate in Its Posttranscriptional Iron ... Corrigendum to "α-Actinin TvACTN3 of Trichomonas vaginalis Is an RNA-Binding Protein That Could Participate in Its ...
Specificity of α-actinin-2 antibodies (4B2) and α-actinin-3 antibodies (5B2).A, COS-7 cells transfected with α-actinin-2 or α- ... α-Actinin-2 and α-actinin-3 expression constructs were prepared by subcloning the full-length cDNAs for α-actinin-2 or α- ... actinin-3 expression constructs were stained with4B2, which specifically recognizes α-actinin-2 and not α-actinin-3, or with ... that are specific for α-actinin-3 (Fig. 1A). Similarly, by immunoblotting, 4B2 recognized α-actinin-2 but not α-actinin-3 ...
... actinin, alpha 1) for IHC-P, WB. Anti-alpha Actinin 1 pAb (GTX103240) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance ... actinin 1 smooth muscle antibody, alpha-actinin cytoskeletal isoform antibody, non-muscle alpha-actinin-1 antibody, "actinin, ... actinin, alpha 1. Background. Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of ... alpha Actinin 1 antibody detects alpha Actinin 1 protein by western blot analysis. Various whole cell extracts (30 µg) were ...
Anti-sarcomeric alpha Actinin mAb (GTX29465) is tested in Human, Mouse, Dog, Pig, Rat, Zebrafish, Cat, Fish, Goat, Hamster, ... sarcomeric alpha Actinin antibody [EA-53] (actin, gamma 1) for ICC/IF, IHC-Fr, IHC-P, IHC-Wm. ... Green: sarcomeric alpha actinin stained by sarcomeric alpha Actinin antibody [EA-53] (GTX29465) diluted at 1:1000. Blue: DAPI ... This antibody is specific for alpha-skeletal muscle actinin and alpha-cardiac actinin. It stains Z lines and dots in stress ...
The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. ... There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to ... Product Description for Actinin alpha (pan). Rabbit anti Human, Mouse, Rat Actinin alpha (pan).. Presentation: Aff - Purified. ... Recommended Secondary Antibodies for Actinin alpha (pan) (9 products). Catalog No.. Host. Clone/Iso.. Pres.. React.. ...
  • Actinin is a microfilament protein. (wikipedia.org)
  • Alpha-actinin-3, also known as alpha-actinin skeletal muscle isoform 3 or F-actin cross-linking protein, is a protein that in humans is encoded by the ACTN3 gene. (wikipedia.org)
  • Alpha-actinin is an actin-binding protein with multiple roles in different cell types. (wikipedia.org)
  • The X homozygous genotype is caused by a C to T transition in exon 16 of the ACTN3 gene, which causes a transformation of an arginine base (R) to a premature stop codon (X) resulting in the rs1815739 mutation causing no production of the alpha-actinin 3 protein in muscle fibers. (wikipedia.org)
  • The 577XX polymorphism causes no production of alpha-actinin 3 protein which is essential in fast twitch muscle fibers. (wikipedia.org)
  • These findings evidence alpha-actinin involvement in sarcomeric development, suggesting for this protein an important role in stabilizing the muscle contractile apparatus. (nih.gov)
  • In this study, we investigated physical and functional interactions between TRPP3 and alpha-actinin, an actin-bundling protein known to regulate several types of ion channels. (nih.gov)
  • Each Alpha Actinin 2 Peptide and Alpha Actinin 2 Protein is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • Actin-binding protein alpha-actinin 4 (ACTN4) is a transcriptional co-activator of RelA/p65 sub-unit of NF-kB. (thefreedictionary.com)
  • Our group at the University of California at Santa Barbara (UCSB) has recently reported on the structure of filamentous (F) actin complexed with the actin cross-linking protein a -actinin (1). (stanford.edu)
  • When filamentous actin is allowed to mix in-vitro with a -actinin, an actin cross-linking protein purified from cells, a remarkable new type of biologically inspired polymer-network is spontaneously formed with fundamentally new properties. (stanford.edu)
  • Zusätzlich bieten wir Ihnen alpha Actinin Antikörper (16) und alpha Actinin Proteine (3) und viele weitere Produktgruppen zu diesem Protein an. (antikoerper-online.de)
  • Thus α-actinin-2 is appropriately positioned at the ultrastructural level to function as a postsynaptic-anchoring protein for NMDA receptors. (jneurosci.org)
  • Given that α-actinins are actin-binding proteins, we hypothesized that α-actinin-2 might function as a bridging protein between NMDA receptors and actin at postsynaptic sites. (jneurosci.org)
  • Recombinant protein encompassing a sequence within the center region of human alpha Actinin 1. (genetex.com)
  • alpha Actinin 1 antibody detects alpha Actinin 1 protein by western blot analysis. (genetex.com)
  • Each α-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. (acris-antibodies.com)
  • This antibody detects endogenous levels of Actinin-α1/2/3/4 protein. (acris-antibodies.com)
  • Here we report our finding of an interaction between the cytoplasmic domain of beta 1 integrin and the actin-binding protein alpha-actinin. (pubmedcentralcanada.ca)
  • One protein at approximately 100 kD was identified by immunoblot analysis as alpha- actinin. (pubmedcentralcanada.ca)
  • Feramisco JR, Burridge K. A rapid purification of alpha-actinin, filamin, and a 130,000-dalton protein from smooth muscle. (pubmedcentralcanada.ca)
  • Jr Protein sequence of DMD gene is related to actin-binding domain of alpha-actinin. (pubmedcentralcanada.ca)
  • Dictyostelium alpha-actinin is a Ca(2+)-regulated F-actin cross-linking protein. (rupress.org)
  • A yeast two-hybrid screening revealed that DmG9a interacts with Tungus, a LIM-domain protein associated with α-Actinin. (uio.no)
  • Intriguingly, approximately 16 % of people worldwide are homozygous for a nonsense mutation in ACTN3 that abolishes actinin-3 protein expression. (springer.com)
  • Phospholipid binding to the ABD causes a conformational change, resulting in the "opening" of the structure that allows EF3/4 to bind the sarcomeric organizer protein titin [ 5 , 6 ] - a potential mechanism to regulate titin and actinin integration into the muscle Z-disk. (springer.com)
  • Deficiency of the fast-twitch muscle protein alpha-actinin-3 due to homozygosity for a nonsense polymorphism (R577X) in the ACTN3 gene is common in humans. (garvan.org.au)
  • α-Actinin, the second protein studied, mainly acts as an actin crosslinking protein. (helsinki.fi)
  • By comparing α-actinin protein distribution in wild type and Actn Δ233 mutant animals, it could be concluded that non-muscle α-actinin is the only isoform expressed in young embryos, in the embryonic central nervous system and in various actin-rich structures of the ovarian germline cells. (helsinki.fi)
  • The population of α-actinin protein present in non-muscle cells of the Actn Δ233 mutant is referred to as FC-α-actinin (Follicle Cell). (helsinki.fi)
  • Alpha-actinin protein: rabbit skeletal muscle (Cat. (cytoskeleton.com)
  • α-Actinin release from the myofibrillar protein fraction to the sarcoplasm can be considered as an accurate proteolysis index in seafood muscle. (csic.es)
  • The actin filament severing protein actophorin promotes the formation of rigid bundles of actin filaments crosslinked with alpha-actinin. (rupress.org)
  • Finally, we have determined that the interaction between LPP and α-actinin, an actin cross-linking protein, is necessary for TGFβ-induced migration and invasion of ErbB2-expressing breast cancer cells. (biologists.org)
  • Deleting this region resulted in a significant decrease in the recruitment of both filamentous actin and the actin binding protein α-actinin. (asm.org)
  • Considerably less is known about the contribution of the intracellular domains, although the amino-terminal domain has been shown to bind directly to the actin binding and cross-linking protein α-actinin ( 28 ). (asm.org)
  • Western immunoblot analysis confirmed that the 100-kD protein is α-actinin. (rupress.org)
  • The results of the in vitro protein binding studies are supported by double-label indirect immunofluorescence experiments that demonstrate a colocalization of CRP1 and α-actinin along the actin stress fibers of CEF and smooth muscle cells. (rupress.org)
  • α-Actinin 4 will be a good marker protein to examine the relation between O-GlcNAcylation and diabetic nephropathy. (elsevier.com)
  • Actinin is a F-actin cross linking protein and is thought to anchor actin to a variety of intracellular structures. (biosensis.com)
  • We demonstrate by protein interaction analysis that two types of titin interactions are involved in the assembly of α‐actinin into the Z‐disk. (embopress.org)
  • A ruler‐like protein, containing variable numbers of specific binding sites for other Z‐disk components, could control the number of α‐actinin molecules and their spacing. (embopress.org)
  • To elucidate the protein composition of the nemaline bodies present in the muscle fibres of patients with congenital nemaline myopathy (CNM), we studied muscle biopsies with monoclonal antibodies against α-actinin and desmin in combination with a modified Gomori trichrome method. (cf.ac.uk)
  • Synthetic phospho-peptide, corresponding to a region within amino acids 140-190 of Human alpha Actinin 4 conjugated to a carrier protein. (abcam.cn)
  • Phospho-α-actinin 4 (Tyr-4) synthetic peptide (coupled to carrier protein) corresponding to amino acids in the N-terminus of human α-actinin 4. (ecmbio.com)
  • The antibody detects a 100 kDa* protein corresponding to the molecular mass of α-actinin 4 on SDS-PAGE immunoblots of human A431 cells treated with pervanadate, but is not observed after akaline phosphatase treatment. (ecmbio.com)
  • This work identifies the actinin rod domain as the predominant domain that influences actinin localisation however localisation is likely to be effected by the entire actinin protein. (ucc.ie)
  • ACTN3 (Actinin Alpha 3) is a Protein Coding gene. (genecards.org)
  • Human recombinant protein fragment corresponding to amino acids 650-893 of human alpha-actinin (NP_001093) produced in E.coli. (biosave.com)
  • In the present study, we reported the identification of α-actinin-2, an actin-crosslinking protein, as a potential angiogenin-interacting partner by yeast two-hybrid screening. (elsevier.com)
  • Using a yeast two-hybrid screen, we identified α-actinin, a major F-actin-cross-linking protein, as a binding partner for the C-terminal domain of the adenosine A2A receptor (A2AR). (cardiff.ac.uk)
  • By using Western blotting, immunofluorescence and immuno-gold labeling, a novel α-actinin-like protein was found in pollen and pollen tubes of Lilium davidii, a model system for cytoskeleton and Golgi apparatus study of plant. (naver.com)
  • As measured by Western blotting, the molecular mass of the α-actinin-like protein was about 80 kDa. (naver.com)
  • Under confocal laser scanning microscopy after immunofluorescence labeling, the distribution of the α-actinin-like protein appeared punctated in the cytoplasm of the pollen and pollen tubes. (naver.com)
  • In addition, some fraction of the α-actinin-like protein was found to co-distribute with F-actin bundles in the pollen tubes. (naver.com)
  • Additional studies with immuno-gold labeling and transmission electron microscopy revealed that the α-actinin-like protein bound mainly to the membranes of Golgi-associated vesicles. (naver.com)
  • These results suggest that the novel α-actinin-like protein is a BFA-sensitive protein on the membranes of Golgi-associated vesicles, and may participate in Golgi-associated vesicles budding and/or sorting, together with actin microfilaments. (naver.com)
  • Alpha-actinin (alpha-actinin-2) is a protein which links the NR1 and NR2B subunits of N-methyl-D-aspartate (NMDA) glutamate receptors to the actin cytoskeleton. (uab.edu)
  • Because of the importance of NMDA receptors in modulating the function of the striatum, we have examined the localization of alpha-actinin-2 protein and mRNA in striatal neurons, and its biochemical interaction with NMDA receptor subunits present in the rat striatum. (uab.edu)
  • Immunoprecipitation of membrane protein extracts showed that alpha-actinin-2 is present in heteromeric complexes of NMDA subunits, but is not associated with AMPA receptors in the striatum. (uab.edu)
  • These data demonstrate that alpha-actinin-2 is a very abundant striatal protein, but exhibits cellular specificity in its expression, with very high levels in substance-P-containing projection neurons, and very low levels in somatostatin and neuronal nitric oxide synthase interneurons. (uab.edu)
  • Despite the high expression of this protein in the striatum, only a minority of NMDA receptors are linked to alpha-actinin-2. (uab.edu)
  • The alpha-actinin-3 protein (ACTN3) gene is important for muscle composition. (issaonline.com)
  • This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. (wikidoc.org)
  • This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. (thermofisher.com)
  • This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. (genetex.com)
  • In this work, novel α-actinin deletion alleles, including Actn Δ233 , were generated, which specifically disrupted the transcript encoding the non-muscle α-actinin isoform. (helsinki.fi)
  • This antibody reacts specifically with alpha actinin isoform #1. (biosensis.com)
  • The predicted length of human actinin (isoform #1) is 892 residues and a MW of 103 kDa. (biosensis.com)
  • Actinin-4 is the predominant isoform reported to be associated with the cancer phenotype. (ucc.ie)
  • This work characterises a number of commercially available actinin antibodies and in doing so, identifies actinin-1, -2 and -4 isoform-specific antibodies that enabled studies of actinin expression and localisation. (ucc.ie)
  • This work finds that the Ca++-insensitive variant of actinin-4 is expressed only in the nervous system and thus cannot be regarded as a smooth muscle isoform, as is the case for the Ca++-insensitive variant of actinin-1. (ucc.ie)
  • Actin-binding properties of actinin-1 and -4 are similar and are unlikely to explain isoform-specific functions. (ucc.ie)
  • The alpha-actinin-2 mRNA is also present in enkephalinergic projection neurons and interneurons expressing parvalbumin, choline acetyl transferase and the 67-kDa isoform of glutamic acid decarboxylase, but was not detected in somatostatin-expressing interneurons. (uab.edu)
  • The non-sarcomeric α-actinins, encoded by ACTN1 and ACTN4, are widely expressed. (wikipedia.org)
  • Mutations in the Alpha-actinin-4 gene (ACTN4) cause a rare form of familial focal segmental glomerulosclerosis in humans. (biomedcentral.com)
  • ELISA kit for Human ACTN4 (Actinin. (antibody-antibodies.com)
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Actinin Alpha 4 (ACTN4) were tested 20 times on one plate, respectively. (biomatik.com)
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Actinin Alpha 4 (ACTN4) were tested on 3 different plates, 8 replicates in each plate. (biomatik.com)
  • This assay has high sensitivity and excellent specificity for detection of Actinin Alpha 4 (ACTN4). (biomatik.com)
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actinin Alpha 4 (ACTN4). (biomatik.com)
  • Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actinin Alpha 4 (ACTN4). (biomatik.com)
  • After TMB substrate solution is added, only those wells that contain Actinin Alpha 4 (ACTN4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. (biomatik.com)
  • The concentration of Actinin Alpha 4 (ACTN4) in the samples is then determined by comparing the O.D. of the samples to the standard curve. (biomatik.com)
  • Actinin, alpha 2 , also known as ACTN2 , is a human gene . (wikidoc.org)
  • MBS015435 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Actinin Alpha 2 (ACTN2) ELISA Kit target analytes in biological samples. (mybiosource.com)
  • Taken together, alpha-actinin not only attaches TRPP3 to the cytoskeleton but also up-regulates TRPP3 channel function. (nih.gov)
  • suggesting that α-actinin-2 may function to attach NMDA receptors to the actin cytoskeleton. (jneurosci.org)
  • There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. (acris-antibodies.com)
  • Follicle cells genetically manipulated to lack all α-actinin isoforms failed to remodel their cytoskeleton and translocate Ena to the posterior of the cell, while the actin fibres as such were not affected. (helsinki.fi)
  • actinin in the AIS is dependent on the integrity of the actin cytoskeleton. (upm.es)
  • actinin and the actin cytoskeleton are important components of the cisternal organelle that are probably required to stabilize the AIS. (upm.es)
  • To determine whether the α-actinin binding domain of CRP1 would localize to the actin cytoskeleton in living cells, expression constructs encoding epitope-tagged full-length CRP1, CRP1-LIM1(aa 1-107), or CRP1-LIM2 (aa 108-192) were microinjected into cells. (rupress.org)
  • Collectively these data demonstrate that the NH 2 -terminal part of CRP1 that contains the α-actinin-binding site is sufficient to localize CRP1 to the actin cytoskeleton. (rupress.org)
  • The rod-shaped α-actinin proteins are central to the organisation of the cytoskeleton as they bind and crosslink actin [ 20 ]. (biomedcentral.com)
  • May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin (By similarity). (nih.gov)
  • Actinins are cytoskeleton proteins that cross-link actin filaments. (ucc.ie)
  • Immunofluorescence in undifferentiated PromoCell cardiomyocytes (Line D) shows only diffuse cytoplasmic expression of sarcomeric actinin (green) with little or no co-localization with the actin cytoskeleton (red). (mssm.edu)
  • Immunofluorescence in PromoCell cardiomycytes (Line D) differentiated for 4 weeks shows increased focal expression of sarcomeric actinin (green) that strongly co-localizes with adhesions sites and the actin cytoskeleton (red). (mssm.edu)
  • These findings suggest an α-actinin-dependent association between the actin cytoskeleton and A2AR trafficking. (cardiff.ac.uk)
  • R577X) has been identified in the ACTN3 gene which results in a deficiency of alpha-actinin 3 in a significant proportion of the population. (wikipedia.org)
  • ACTN3 has been shown to interact with Actinin, alpha 2. (wikipedia.org)
  • In an Actn3 knockout mouse model, alpha-actinin-3 deficiency is associated with a shift in the characteristics of fast, glycolytic 2B muscle fibers towards a slow phenotype, with decreased muscle mass and fiber diameter, slower contractile properties, increased fatigue resistance, and an increase in oxidative enzyme activity. (nih.gov)
  • The effect of alpha-actinin 3 (ACTN3) and angiotensin-converting enzyme (ACE) on endurance or sprint athletic performance have been documented. (thefreedictionary.com)
  • In this regard, one of the genes that may influence these phenotypes is the alpha-actinin 3 (ACTN3) gene (Clarkson et al. (thefreedictionary.com)
  • To further explore these potential age-related effects and the underlying mechanisms, we examined the effect of alpha-actinin-3 deficiency in aging male and female Actn3 knockout (KO) mice (2, 6, 12, and 18 months). (garvan.org.au)
  • Aim: The R577X polymorphism of the alpha-actinin-3 (ACTN3) gene and the IVS1-6G>A polymorphism of the ciliary neurotrophic factor (CNTF) gene have been associated with a favourable muscle phenotype (more muscle fibres with high glycolytic activity), reduced predisposition for congenital dystrophy and resistance to sarcopenia in old age. (minervamedica.it)
  • Actinins are one of the major actin cross-linking proteins found in virtually all cell types and are the ancestral proteins of a larger family that includes spectrin, dystrophin and utrophin. (biomedcentral.com)
  • Interaction of iodinated vinculin, metavinculin and alpha-actinin with cytoskeletal proteins. (nih.gov)
  • Iodinated vinculin, metavinculin and alpha-actinin were used to probe the interaction of these proteins with electrophoretically separated cytoskeletal proteins. (nih.gov)
  • We offer Alpha Actinin 2 Peptides and Alpha Actinin 2 Proteins for use in common research applications: Blocking/Neutralizing, Control. (novusbio.com)
  • Our Alpha Actinin 2 Peptides and Alpha Actinin 2 Proteins can be used in a variety of model species: Human. (novusbio.com)
  • Choose from our Alpha Actinin 2 Peptides and Proteins. (novusbio.com)
  • Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. (wikidoc.org)
  • The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. (acris-antibodies.com)
  • Proteomic analysis of the immunoreactive bands, involving MALDI and MS/MS techniques, revealed the presence of four proteins distinguishable by their mass: alpha fodrin, alpha actinin 1, creatine kinase, and CNPase. (mcponline.org)
  • A number of cytoskeletal-associated proteins that are concentrated in focal contacts, namely alpha-actinin, vinculin, talin, and integrin, have been shown to interact in vitro such that they suggest a potential link between actin filaments and the membrane. (pubmedcentralcanada.ca)
  • Actinins are dimeric actin filament cross-linking proteins. (springer.com)
  • Results: O-GlcNAcylated proteins that changed significantly in the degree of O-GlcNAcylation were identified as cytoskeletal proteins (α-actin, α-tubulin, α-actinin 4, myosin) and mitochondrial proteins (ATP synthase β, pyruvate carboxylase). (elsevier.com)
  • α-Actinins are widely expressed cytoskeletal proteins that cross-link actin filaments through anti-parallel homodimers of the rod domains. (ecmbio.com)
  • Taken together this data suggests that actinin-1 and -4 cannot be viewed as distinct entities from each other but rather as proteins that can exist in both homodimeric and heterodimeric forms. (ucc.ie)
  • Finally, this work employs yeast two-hybrid and proteomic approaches to identify actinin-interacting proteins. (ucc.ie)
  • The observation of alternative splice variants of actinin-1 and -4 combined with the observed potential of these proteins to form homodimers and heterodimers suggests that homodimers and heterodimers with novel actin-binding properties and interaction networks may exist. (ucc.ie)
  • Western blot of these Tvα-actinin 2 proteins with pooled patients' sera indicated that #2 and #3, but not #1, reacted with those sera. (yonsei.ac.kr)
  • Fractionation experiments indicated the presence of Tvα-actinin 2 in cytoplasmic, membrane, and secreted proteins of T. vaginalis. (yonsei.ac.kr)
  • When the pollen tubes were treated with Brefeldin A (BFA), the α-actinin-like proteins were dispersed into the cytoplasm, and the growth of pollen tubes was inhibited. (naver.com)
  • There are four human α-actinin (ACTN1-4) family members, encoding closely related actin crosslinking proteins. (biomedcentral.com)
  • The main objective of the present study was to develop a specific enzyme-linked immunosorbent assay (ELISA), based on the use of a monoclonal antibody against -actinin to evaluate the degree of proteolysis in two different chilled fish species - European hake (Merluccius merluccius) and turbot (Psetta maxima) - kept under two different storage systems: flake ice and slurry ice. (csic.es)
  • The membrane was incubated with mouse anti- Alpha-Actinin antigen affinity purified monoclonal antibody (Catalog # MA1104) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. (bosterbio.com)
  • Purified alpha-actinin mouse monoclonal antibody, clone 7A4 from OriGene Technologies Inc. (biosave.com)
  • Second, α-actinin-2 was coimmunoprecipitated with angiogenin by anti-angiogenin monoclonal antibody. (elsevier.com)
  • The following antibody was used in this experiment: alpha Actinin 4 Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-22259, RRID AB_11157252. (thermofisher.com)
  • It remains to be determined whether the TRPP3-alpha-actinin interaction is relevant to acid sensing and other functions in neuronal and non-neuronal cells. (nih.gov)
  • A single interaction of α-actinin and titin turns out to be surprisingly weak if force is applied. (pnas.org)
  • Apart from a direct interaction between actin filaments and titin at the Z-disk edge ( 13 ), the most prominent candidate for the anchoring of titin within the Z-disk is its interaction with α-actinin ( Fig. 1 B ) ( 6 , 12 , 14 ). (pnas.org)
  • Interaction between α-actinin EF3-4 and titin Z-repeat 7 in PullA-T7 geometry. (pnas.org)
  • In order to understand the interaction between actin and a -actinin which leads to this previously overlooked self-assembling system, small angle x-ray diffraction (SAXRD) experiments were performed on beam lines 4-2 and 10-2 at SSRL to probe the local structural organization within the bundles (1). (stanford.edu)
  • This differential regional expression implies that glutamatergic synapses in various parts of the brain differ with respect to their α-actinin-2 content and thus, potentially, the extent of possible interaction between α-actinin-2 and the NMDA receptor. (jneurosci.org)
  • We have shown that the CRP1-α-actinin interaction is direct, specific, and saturable in both solution and solid-phase binding assays. (rupress.org)
  • The K d for the CRP1-α-actinin interaction is 1.8 ± 0.3 μM. (rupress.org)
  • Further evidence demonstrated that activation of PI 3-kinase by PDGF induced a decrease in the association of α-actinin with the integrin β subunit, and that PtdIns (3,4,5)-P 3 could disrupt this interaction in vitro. (elsevier.com)
  • The interaction between titin and alpha-actinin involves repeating approximately 45 amino acid sequences (Z-repeats) near the N-terminus of titin and the C-lobe of the C-terminal calmodulin-like domain of alpha-actinin. (mpg.de)
  • Since members of α-actinin family play pivotal roles in cell proliferation, migration, and invasion, the interaction between α-actinin-2 and angiogenin may underline one possible mechanism of angiogenin in angiogenesis. (elsevier.com)
  • Colocalization, co-immunoprecipitation, and pull-down experiments showed a close and specific interaction between A2AR and α-actinin in transfected HEK-293 cells and also in rat striatal tissue. (cardiff.ac.uk)
  • An interaction between zyxin and alpha-actinin. (naver.com)
  • alpha-actinin-2 in rat striatum: localization and interaction with NMDA glutamate receptor subunits. (uab.edu)
  • Your search returned 214 Alpha-Actinin-4 Antibodies across 33 suppliers. (biocompare.com)
  • This study investigated the overall clinical impact of anti-α-actinin antibodies in patients with pre-selected autoimmune diseases and in a random group of anti-nuclear antibody (ANA)-positive individuals. (biomedcentral.com)
  • The relation of anti-α-actinin antibodies with lupus nephritis and anti-double-stranded DNA (anti-dsDNA) antibodies represented a particular focus for the study. (biomedcentral.com)
  • Using a cross-sectional design, the presence of antibodies to α-actinin was studied in selected groups, classified according to the relevant American College of Rheumatology classification criteria for systemic lupus erythematosus (SLE) ( n = 99), rheumatoid arthritis (RA) ( n = 68), Wegener's granulomatosis (WG) ( n = 85), and fibromyalgia (FM) ( n = 29), and in a random group of ANA-positive individuals ( n = 142). (biomedcentral.com)
  • Sera from SLE, RA, and Sjøgren's syndrome (SS) patients had significantly higher levels of anti-α-actinin antibodies than the other patient groups. (biomedcentral.com)
  • Using the geometric mean (± 2 standard deviations) in FM patients as the upper cutoff, 20% of SLE patients, 12% of RA patients, 4% of SS patients, and none of the WG patients were positive for anti-α-actinin antibodies. (biomedcentral.com)
  • In the random ANA group, 14 individuals had anti-α-actinin antibodies. (biomedcentral.com)
  • Antibodies binding to α-actinin were detected in 20% of SLE patients but were not specific for SLE. (biomedcentral.com)
  • Thus, the frequent combination of anti-filamentous and anti-α-actinin antibodies seems to be the hallmark of activity in AIH-1. (elsevier.com)
  • Immunofluorescence assays of two different forms of T. vaginalis (trophozoites and amoeboid forms), using anti-Tvα-actinin 2 antibodies, showed localization of Tvα-actinin 2 close to the plasma membranes of the amoeboid form. (yonsei.ac.kr)
  • Binding of fluorescence-labeled Trichomonas to vaginal epithelial cells and prostate cells was decreased in the antibody blocking experiment using anti-Tvα-actinin 2 antibodies. (yonsei.ac.kr)
  • Pretreatment of T. vaginalis with anti-rTvα-actinin 2 antibodies also resulted in reduction in its cytotoxicity. (yonsei.ac.kr)
  • Defects in the gene encoding α-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure. (acris-antibodies.com)
  • Invertebrates have a single actinin-encoding ACTN gene, while mammals have four. (springer.com)
  • Muscle and non-muscle cells utilise different α-actinin isoforms, which in Drosophila are produced by alternative splicing of a single gene. (helsinki.fi)
  • Title: Arf6 guanine-nucleotide exchange factor, cytohesin-2, interacts with actinin-1 to regulate neurite extension. (nih.gov)
  • In reciprocal mapping studies, we showed that α-actinin interacts with CRP1-LIM1, a deletion fragment that contains the NH 2 -terminal 107 amino acids (aa) of CRP1. (rupress.org)
  • Titin interacts via a single binding site with the two central spectrin‐like repeats of the outermost pair of α‐actinin molecules. (embopress.org)
  • In the Z-disk, it interacts with alpha-actinin homodimers that are a principal component of the Z-filaments linking actin filaments. (mpg.de)
  • At least 4 isoforms of alpha actinin have been found. (biosensis.com)
  • Evolution of the actinin family resulted in the formation of Ca++-insensitive muscle isoforms (actinin-2 and- 3) and Ca++-sensitive non-muscle isoforms (actinin-1 and -4) with regard to their actin-binding function. (ucc.ie)
  • Using the gel overlay technique, we detected strong binding of 125I-vinculin and 125I-metavinculin to alpha-actinin, 175 kDa polypeptide, talin, vinculin and metavinculin themselves, and moderate binding to actin. (nih.gov)
  • 125I-alpha-actinin was capable of interacting with vinculin and metavinculin. (nih.gov)
  • The specific binding of 125-I-alpha-actinin to vinculin and metavinculin immobilized on a polysterene surface was also demonstrated. (nih.gov)
  • We suggest that the ability of vinculin and alpha-actinin to form a complex may be realized in microfilament-membrane linkages. (nih.gov)
  • This complex consists of alpha-actinin and talin which bind to the cytoplasmic C-terminal tail of the integrin and paxillin and vinculin which bind to talin and alphctinin. (thefreedictionary.com)
  • α-Actinin cross-links the actin filament networks and associates the network to focal adhesion sites through binding of talin and vinculin. (ecmbio.com)
  • Loss of this close apposition between the cell membrane and the extracellular matrix coincided with a redistribution of α-actinin and vinculin from the focal adhesion complex to the Triton X-100 - soluble fraction. (elsevier.com)
  • Auf www.antikoerper-online.de finden Sie aktuell 16 alpha-Actinin Skeletal Muscle (ACTN) ELISA Kits von 3 unterschiedlichen Herstellern. (antikoerper-online.de)
  • In the Actn Δ233 mutant, α-actinin was detected not only in muscle tissue, but also in embryonic epidermal cells and in certain follicle cell populations in the ovaries. (helsinki.fi)
  • A splice variant of actinin-4 (Actn-4sv) was recently found to be an excellent biomarker of neuroendocrine neoplasms of the lung. (elsevier.com)
  • Formalin fixed paraffin embedded human skeletal muscle stained with α-Actinin (sarcomeric) antibody. (genetex.com)
  • Green: sarcomeric alpha actinin stained by sarcomeric alpha Actinin antibody [EA-53] (GTX29465) diluted at 1:1000. (genetex.com)
  • In the most immature cells used, alpha-actinin was found in diffuse aggregates, some of which displayed sarcomeric periodicity. (rupress.org)
  • The sarcomeric Z‐disk, the anchoring plane of thin (actin) filaments, links titin (also called connectin) and actin filaments from opposing sarcomere halves in a lattice connected by α‐actinin. (embopress.org)
  • The combination of our biochemical and ultrastructural data now allows a molecular model of the sarcomeric Z‐disk, where overlapping titin filaments and their interactions with the α‐actinin rod and C‐terminal domain can account for the essential ultrastructural features. (embopress.org)
  • Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Sarcomeric Alpha Actinin aa 1-100 (N terminal). (abcam.co.jp)
  • abstract = "Highly purified α actinin can be made by using the low ionic strength extraction procedure and then subjecting the crude α actinin fraction obtained with this extraction procedure to successive chromatography on DEAE cellulose and hydroxyapatite. (elsevier.com)
  • Molecular genetics of Drosophila alpha-actinin: mutant alleles disrupt Z disc integrity and muscle insertions. (pubmedcentralcanada.ca)
  • More broadly though, this structure presents a picture of the intimate interactions between the subunits in the actinin dimer, providing a framework to better understand the molecular details of actin cross-linking and its regulation in all actinins and perhaps in spectrins too. (springer.com)
  • In 6 M guanidine HCl, hydroxyapatite purified α actinin has a molecular weight of 106,000 ± 6,300 as determined by sedimentation equilibrium and a molecular weight of 100,000 as determined by a calibrated 4% agarose gel permeation column. (elsevier.com)
  • SDS polyacrylamide gel electrophoresis gives a molecular weight of 96,000 to 100,000 for hydroxyapatite purified α actinin. (elsevier.com)
  • By assuming 45% hydration and a molecular weight of 206,000, dimensions of approximately 40 x 500Å can be calculated for the α actinin molecule by using either So 20 ,w, Do 20 ,w, intrinsic viscosity, or a calibrated 6% agarose gel permeation column. (elsevier.com)
  • Bundling of actin filaments by alpha-actinin depends on its molecular length. (naver.com)
  • The antibody of β-actinin did not cross-react to cytoplasmic β-actinin (molecular weight, 80, 000 daltons) found in kidney. (elsevier.com)
  • The calmodulin-like domain of α-actinin binds to the Z-repeats of titin. (pnas.org)
  • As α-actinin binds directly to the EHEC O157:H7 amino terminus, these data suggest that its recruitment is dependent on pedestal formation. (asm.org)
  • Recombinant actin-binding domain (residues 2-269) binds actin filaments with a K(d) of 1 μM at 25°C, 20 times stronger than actin-binding domain produced by thermolysin digestion of native α-actinin (residues 25-257). (elsevier.com)
  • We also found that PtdIns (3,4,5)-P 3 binds to α-actinin in PDGF-treated cells. (elsevier.com)
  • Structural analysis of homologous repeated domains in alpha-actinin and spectrin. (pubmedcentralcanada.ca)
  • α-Actinins contain three conserved domains that include an N-terminal actin binding domain, four spectrin-like repeats in the central region, and a C-terminal calmodulin binding domain. (ecmbio.com)
  • The sequence of chick alpha-actinin reveals homologies to spectrin and calmodulin. (naver.com)
  • α-Actinin monomers contain three distinct domains: an N-terminal actin binding domain (ABD), four spectrin-like repeats (SRs), and a C-terminal EF hands (calmodulin like domain). (biomedcentral.com)
  • Surprisingly, this work reveals that actinin-1/-4 heterodimers, rather than homodimers, are the most abundant form of actinin in many cancer cell lines. (ucc.ie)
  • 2009) Human angiotensin-converting enzyme I/D and alpha-actinin 3 R577X genotypes and muscle functional and contractile properties. (thefreedictionary.com)
  • Synthetic peptide, corresponding to amino acids 30-60 of Human Actinin-α1. (acris-antibodies.com)
  • Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11313-2-AP( alpha Actinin antibody at dilution of 1:200 (under 40x lens). (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11313-2-AP( alpha Actinin antibody at dilution of 1:200 (under 10x lens). (ptglab.com)
  • This work also identifies a previously unreported exon 19a+19b expressing variant of actinin-4 in human skeletal muscle. (ucc.ie)
  • Flow cytometry, ligand-binding immunoblotting assay, and observation by fluorescence microscopy were used to detect the binding of recombinant Tvα-actinin 2 to human epithelial cell lines. (yonsei.ac.kr)
  • Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Alpha-actinin 1(GTX103240) antibody at 1:100 dilution. (genetex.com)
  • Alpha-Actinin was detected in paraffin-embedded section of mouse lung tissues. (bosterbio.com)
  • A concentration of 3.5-7.0 g/ml is recommended to detect alpha actinin in formalin fixed and paraffin embedded tissues. (biosensis.com)
  • In this paper, we show with direct mechanical single-molecule measurements that an array of titin/α-actinin bonds composes a dynamic network that can provide stable anchoring, maintaining the integrity of the muscle Z-disk even under load. (pnas.org)
  • One of the main candidates for anchoring titin in the Z-disk is the actin cross-linker α-actinin. (pnas.org)
  • Our results suggest a model where multiple α-actinin/Z-repeat interactions cooperate to ensure long-term stable titin anchoring while allowing the individual components to exchange dynamically. (pnas.org)
  • B ) Arrangement of actin, titin, telethonin, and α-actinin within the Z-disk. (pnas.org)
  • In the central Z‐disk, titin can interact with multiple α‐actinin molecules via their C‐terminal domains. (embopress.org)
  • These interactions allow the assembly of a ternary complex of titin, actin and α‐actinin in vitro , and are expected to constrain the path of titin in the Z‐disk. (embopress.org)
  • In thick skeletal muscle Z‐disks, titin filaments cross over the Z‐disk centre by ∼30 nm, suggesting that their α‐actinin‐binding sites overlap in an antiparallel fashion. (embopress.org)
  • The conformation of Z-repeat 7 (ZR7) of titin when complexed with the 73-amino acid C-terminal portion of alpha-actinin (EF34) was studied by heteronuclear NMR spectroscopy using (15)N-labeling of ZR7 and found to be helical over a stretch of 18 residues. (mpg.de)
  • Light microscopy of sections immunolabelled for a-actinin showed a cross-striation of the muscle fibres corresponding to the Z band pattern, focal thickening of the Z bands and additional reactivity with a granular pattern corresponding to the presence of nemaline bodies. (cf.ac.uk)
  • To test this reactivity, an anti-α-actinin enzyme-linked immunosorbent assay was developed, and positivity confirmed by Western blot. (elsevier.com)
  • Each actin filament overlaps with four filaments from the opposite sarcomere, forming a square lattice which is cross‐connected in a zig‐zag pattern by Z‐filaments, assumed to consist of α‐actinin (see Luther, 1991 , and references therein). (embopress.org)
  • We used smooth muscle α-actinin to evaluate the contribution of cross- linker dynamics to the mechanical properties of actin filament networks. (elsevier.com)
  • Xu, J , Wirtz, D & Pollard, TD 1998, ' Dynamic cross-linking by α-actinin determines the mechanical properties of actin filament networks ', Journal of Biological Chemistry , vol. 273, no. 16, pp. 9570-9576. (elsevier.com)
  • Alpha-actinin interactions with syndecan-4 are integral to fibroblast-matrix adhesion and regulate cytoskeletal architecture. (nih.gov)
  • Primary structure of chicken skeletal muscle and fibroblast alpha-actinins deduced from cDNA sequences. (naver.com)
  • Examination of the α-actinin localisation pattern revealed that the basal actin fibres of the main body follicle cells underwent an organised remodelling during the final stages of oogenesis. (helsinki.fi)
  • Electron microscopy of immunolabelled resin embedded sections was used for cytochemical localisation of a-actinin and desmin. (cf.ac.uk)
  • Here we show that α-actinin-2 is localized specifically in glutamatergic synapses in cultured hippocampal neurons. (jneurosci.org)
  • Immunofluorescence and in situ PLA studies revealed that the levels of O-GlcNAcylation of actin, α-actinin 4 and myosin were significantly increased in the glomerulus and the proximal tubule of the diabetic kidney. (elsevier.com)
  • Actinin-4, but not actinin-1, is essential for normal glomerular function in the kidney and and is able to translocate to the nucleus to regulate transcription. (ucc.ie)
  • the frequency of the XX genotype is significantly lower than controls in sprint athletes, and it appears that alpha-actinin-3 deficiency is detrimental to sprint performance. (nih.gov)
  • alpha-Actinin-3 deficiency (XX) is associated with reduced muscle strength/power and enhanced endurance performance in elite athletes and in the general population. (garvan.org.au)
  • α-Actinin-3 deficiency occurs in an estimated 1.5 billion people worldwide, and results in reduced muscle strength and a shift towards a more efficient oxidative metabolism. (semanticscholar.org)
  • How does α-actinin-3 deficiency alter muscle function? (semanticscholar.org)
  • α-Actinin involvement in Z-disk assembly during skeletal muscle C2C12 cells in vitro differentiation. (nih.gov)
  • The TRPP3-alpha-actinin association was documented by co-immunoprecipitation using native cells and tissues, yeast two-hybrid, and in vitro binding assays. (nih.gov)
  • right) Confocal images of Factin complexed with a-actinin in vitro (a-actinin:G-actin=1:25) reveals the formation of a network of bundles with isotropic symmetry and a well-defined mesh size. (stanford.edu)
  • By in vitro domain mapping studies, we have determined that CRP1 associates with the 27-kD actin-binding domain of α-actinin. (rupress.org)
  • A subunit-specific anti-NR1 antibody co-precipitated major fractions of NR2A and NR2B subunits, but only a minor fraction of striatal alpha-actinin-2. (uab.edu)
  • Conversely, alpha-actinin-2 antibody immunoprecipitated only modest fractions of striatal NR1, NR2A and NR2B subunits. (uab.edu)
  • Four α-actinin genes have been discovered in humans with α-actinin 1 and 4 being widely expressed in non-muscle cells. (ecmbio.com)
  • By immunogold electron microscopy, α-actinin-2 is concentrated over the postsynaptic density (PSD) of numerous asymmetric synapses where it colocalizes with NR1 immunoreactivity. (jneurosci.org)
  • Electron microscopy confirmed the presence of α-actinin in the nemaline bodies and Z bands, whereas desmin was only found in intermediate filaments around the Z bands. (cf.ac.uk)
  • α-Actinin is necessary for the attachment of actin filaments to the Z-lines in skeletal muscle cells, and to the dense bodies in smooth muscle cells. (wikipedia.org)
  • In turn, the shift towards a 'slow' muscle phenotype in fast muscle fibers likely explains why loss of alpha-actinin-3 is detrimental to sprint performance. (nih.gov)
  • This antibody is specific for alpha-skeletal muscle actinin and alpha-cardiac actinin. (genetex.com)
  • Using purified heterodimeric chicken smooth muscle integrin (a beta 1 integrin) or the platelet integrin glycoprotein IIb/IIIa complex (a beta 3 integrin), binding of alpha-actinin was also observed in similar solid phase assays, albeit with a lower affinity than was seen using the beta 1 peptide. (pubmedcentralcanada.ca)
  • The Ca(2+)-binding domains in non-muscle type alpha-actinin: biochemical and genetic analysis. (rupress.org)
  • These results provide further support that alpha-actinin-3 deficient individuals may experience faster decline in muscle function with increasing age. (garvan.org.au)
  • In Drosophila , the only function for α-actinin yet known is in the organisation of the muscle sarcomere. (helsinki.fi)
  • At late oogenesis, the main body follicle cells express both non-muscle α-actinin and FC-α-actinin, while the dorsal anterior follicle cells express only non-muscle α-actinin. (helsinki.fi)
  • Moreover, we have shown that α-actinin coimmunoprecipitates with CRP1 from a detergent extract of smooth muscle cells. (rupress.org)
  • The association of CRP1 with α-actinin may be critical for its role in muscle differentiation. (rupress.org)
  • In view of the above, it seems improbable that the intraglomerular presence of (non-muscle) α-actinin would be a specific occurrence in patients with LN, although an Ab response to α-actinin may still be specific for SLE and contribute to LN. (biomedcentral.com)
  • Our results provide direct evidence for the presence of α-actinin in nemaline bodies and a lack of quantitative or qualitative differences between the α-actinin of normal and CNM muscle. (cf.ac.uk)
  • si-RNA- mediated knockdown of actinin-1 and -4 did not affect migration in a number of cell lines highlighting that migration may only require a fraction of total non-muscle actinin levels. (ucc.ie)
  • This significantly alters our view of the non-muscle actinins. (ucc.ie)
  • Stromer, M. H. / Some properties of purified skeletal muscle α actinin . (elsevier.com)
  • for this purpose, an antibody was raised against β-actinin purified from adult chicken breast muscle (pectoralis major). (elsevier.com)
  • They also go on to say "In addition, α-actinin-3 may be evolutionarily optimized for the minimization of damage caused by eccentric muscle contraction. (issaonline.com)