Actins: Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Actin Cytoskeleton: Fibers composed of MICROFILAMENT PROTEINS, which are predominately ACTIN. They are the smallest of the cytoskeletal filaments.Actin Depolymerizing Factors: A family of low MOLECULAR WEIGHT actin-binding proteins found throughout eukaryotes. They remodel the actin CYTOSKELETON by severing ACTIN FILAMENTS and increasing the rate of monomer dissociation.Actin Capping Proteins: Actin capping proteins are cytoskeletal proteins that bind to the ends of ACTIN FILAMENTS to regulate actin polymerization.Cytoskeleton: The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.Phalloidine: Very toxic polypeptide isolated mainly from AMANITA phalloides (Agaricaceae) or death cup; causes fatal liver, kidney and CNS damage in mushroom poisoning; used in the study of liver damage.Thiazolidines: Reduced (protonated) form of THIAZOLES. They can be oxidized to THIAZOLIDINEDIONES.Gelsolin: A 90-kDa protein produced by macrophages that severs ACTIN filaments and forms a cap on the newly exposed filament end. Gelsolin is activated by CALCIUM ions and participates in the assembly and disassembly of actin, thereby increasing the motility of some CELLS.Profilins: A family of low molecular weight proteins that bind ACTIN and control actin polymerization. They are found in eukaryotes and are ubiquitously expressed.Cytochalasin D: A fungal metabolite that blocks cytoplasmic cleavage by blocking formation of contractile microfilament structures resulting in multinucleated cell formation, reversible inhibition of cell movement, and the induction of cellular extrusion. Additional reported effects include the inhibition of actin polymerization, DNA synthesis, sperm motility, glucose transport, thyroid secretion, and growth hormone release.Myosins: A diverse superfamily of proteins that function as translocating proteins. They share the common characteristics of being able to bind ACTINS and hydrolyze MgATP. Myosins generally consist of heavy chains which are involved in locomotion, and light chains which are involved in regulation. Within the structure of myosin heavy chain are three domains: the head, the neck and the tail. The head region of the heavy chain contains the actin binding domain and MgATPase domain which provides energy for locomotion. The neck region is involved in binding the light-chains. The tail region provides the anchoring point that maintains the position of the heavy chain. The superfamily of myosins is organized into structural classes based upon the type and arrangement of the subunits they contain.Bicyclo Compounds, Heterocyclic: A class of saturated compounds consisting of two rings only, having two or more atoms in common, containing at least one hetero atom, and that take the name of an open chain hydrocarbon containing the same total number of atoms. (From Riguady et al., Nomenclature of Organic Chemistry, 1979, p31)Actin-Related Protein 2-3 Complex: A complex of seven proteins including ARP2 PROTEIN and ARP3 PROTEIN that plays an essential role in maintenance and assembly of the CYTOSKELETON. Arp2-3 complex binds WASP PROTEIN and existing ACTIN FILAMENTS, and it nucleates the formation of new branch point filaments.Contractile Proteins: Proteins which participate in contractile processes. They include MUSCLE PROTEINS as well as those found in other cells and tissues. In the latter, these proteins participate in localized contractile events in the cytoplasm, in motile activity, and in cell aggregation phenomena.Pseudopodia: A dynamic actin-rich extension of the surface of an animal cell used for locomotion or prehension of food.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Hominidae: Family of the suborder HAPLORHINI (Anthropoidea) comprising bipedal primate MAMMALS. It includes modern man (HOMO SAPIENS) and the great apes: gorillas (GORILLA GORILLA), chimpanzees (PAN PANISCUS and PAN TROGLODYTES), and orangutans (PONGO PYGMAEUS).Protein Isoforms: Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING.Earth Sciences: Fields of science encompassing studies and research from the disciplines of PHYSICS; CHEMISTRY; BIOLOGICAL SCIENCES; and MATHEMATICS; that are related to the planet EARTH. Subfields include atmospheric chemistry; CLIMATOLOGY; ENVIRONMENTAL SCIENCES; GEOGRAPHY; GEOLOGY; geophysics; METEOROLOGY; OCEANOGRAPHY; PALEONTOLOGY; mineralogy; and seismology.Science: The study of natural phenomena by observation, measurement, and experimentation.Physics: The study of those aspects of energy and matter in terms of elementary principles and laws. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Portal Vein: A short thick vein formed by union of the superior mesenteric vein and the splenic vein.ShoesSweat: The fluid excreted by the SWEAT GLANDS. It consists of water containing sodium chloride, phosphate, urea, ammonia, and other waste products.Sweating: The process of exocrine secretion of the SWEAT GLANDS, including the aqueous sweat from the ECCRINE GLANDS and the complex viscous fluids of the APOCRINE GLANDS.Nutritional Requirements: The amounts of various substances in food needed by an organism to sustain healthy life.Snacks: Foods eaten between MEALTIMES.Orthotic Devices: Apparatus used to support, align, prevent, or correct deformities or to improve the function of movable parts of the body.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.Peptide Termination Factors: Proteins that are involved in the peptide chain termination reaction (PEPTIDE CHAIN TERMINATION, TRANSLATIONAL) on RIBOSOMES. They include codon-specific class-I release factors, which recognize stop signals (TERMINATOR CODON) in the MESSENGER RNA; and codon-nonspecific class-II release factors.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Microfilament Proteins: Monomeric subunits of primarily globular ACTIN and found in the cytoplasmic matrix of almost all cells. They are often associated with microtubules and may play a role in cytoskeletal function and/or mediate movement of the cell or the organelles within the cell.User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.ArchivesDrosophila melanogaster: A species of fruit fly much used in genetics because of the large size of its chromosomes.Nuclear Envelope: The membrane system of the CELL NUCLEUS that surrounds the nucleoplasm. It consists of two concentric membranes separated by the perinuclear space. The structures of the envelope where it opens to the cytoplasm are called the nuclear pores (NUCLEAR PORE).Muscle Proteins: The protein constituents of muscle, the major ones being ACTINS and MYOSINS. More than a dozen accessory proteins exist including TROPONIN; TROPOMYOSIN; and DYSTROPHIN.Drosophila: A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology.Drosophila Proteins: Proteins that originate from insect species belonging to the genus DROSOPHILA. The proteins from the most intensely studied species of Drosophila, DROSOPHILA MELANOGASTER, are the subject of much interest in the area of MORPHOGENESIS and development.Dendritic Spines: Spiny processes on DENDRITES, each of which receives excitatory input from one nerve ending (NERVE ENDINGS). They are commonly found on PURKINJE CELLS and PYRAMIDAL CELLS.CapZ Actin Capping Protein: An actin capping protein that binds to the barbed-ends of ACTIN filaments. It is a heterodimer consisting of an alpha and a beta subunit. It regulates actin assembly by stabilizing actin oligomers for elongation. In SKELETAL MUSCLE, CapZ is localized to the Z-disk.Sperm Capacitation: The structural and functional changes by which SPERMATOZOA become capable of oocyte FERTILIZATION. It normally requires exposing the sperm to the female genital tract for a period of time to bring about increased SPERM MOTILITY and the ACROSOME REACTION before fertilization in the FALLOPIAN TUBES can take place.Spermatogenesis: The process of germ cell development in the male from the primordial germ cells, through SPERMATOGONIA; SPERMATOCYTES; SPERMATIDS; to the mature haploid SPERMATOZOA.Acanthamoeba: A genus of free-living soil amoebae that produces no flagellate stage. Its organisms are pathogens for several infections in humans and have been found in the eye, bone, brain, and respiratory tract.Cell Surface Extensions: Specialized structures of the cell that extend the cell membrane and project out from the cell surface.Cortactin: A microfilament protein that interacts with F-ACTIN and regulates cortical actin assembly and organization. It is also an SH3 DOMAIN containing phosphoprotein, and it mediates tyrosine PHOSPHORYLATION based SIGNAL TRANSDUCTION by PROTO-ONCOGENE PROTEIN PP60(C-SRC).
This gene encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin ... The product of this gene belongs to the actin-binding proteins ADF (Actin-Depolymerizing Factor)/cofilin family. This family of ... Destrin or DSTN (also known as actin depolymerizing factor or ADF) is a protein which in humans is encoded by the DSTN gene. ... Destrin can also sever actin filaments. References. *^ a b c d PDB: 1AK6; Hatanaka H, Ogura K, Moriyama K, Ichikawa S ...
AZI2: encoding protein 5-azacytidine-induced protein 2. *BRK1: SCAR/WAVE actin nucleating complex subunit ... C3orf14-Chromosome 3 open reading frame 14: predicted DNA binding protein.. *C3orf23: encoding protein Uncharacterized protein ... FRA3A encoding protein Fragile site, aphidicolin type, common, fra(3)(p24.2). *FRMD4B encoding protein FERM domain containing ... ZBED2: encoding protein Zinc finger BED-type containing 2. *ZNF9: zinc finger protein 9 (a cellular retroviral nucleic acid ...
Actin-like protein 7A is a protein that in humans is encoded by the ACTL7A gene. The protein encoded by this gene is a member ... which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is ... and expression of two novel actin genes, actin-like-7A (ACTL7A) and actin-like-7B (ACTL7B), from the familial dysautonomia ... Schafer DA, Schroer TA (2000). "Actin-related proteins". Annu. Rev. Cell Dev. Biol. 15: 341-63. doi:10.1146/annurev.cellbio. ...
Actin-like protein 6B is a protein that in humans is encoded by the ACTL6B gene. The protein encoded by this gene is a member ... "Entrez Gene: ACTL6B actin-like 6B". Oma Y, Nishimori K, Harata M (February 2003). "The brain-specific actin-related protein ... which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is ... Harata M, Mochizuki R, Mizuno S (1999). "Two isoforms of a human actin-related protein show nuclear localization and mutually ...
Hartwig JH (1995). "Actin-binding proteins. 1: Spectrin super family". Protein Prof. 2 (7): 703-800. PMID 7584474. Gimona M, ... is a family of actin binding domains found in both cytoskeletal proteins and signal transduction proteins. The domain is about ... However, in calponins there is evidence that the CH domain is not involved in its actin binding activity. Most proteins have ... Calponin-type A comprehensive review of proteins containing this type of actin-binding domains is given in. The CH domain is ...
Actin-like protein 6A is a protein that in humans is encoded by the ACTL6A gene. This gene encodes a family member of actin- ... related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have ... "Alternative splicing products of the gene for a human nuclear actin-related protein, hArpNbeta/Baf53, that encode a protein ... Kuroda Y, Oma Y, Nishimori K, Ohta T, Harata M (November 2002). "Brain-specific expression of the nuclear actin-related protein ...
... large enough to allow the passage of integral membrane proteins; temporal actin filament brackage; membrane molecules have ... that occur when cytoplasmic domains collide with the actin-based membrane skeleton; and anchored-transmembrane protein "pickets ... Hop diffusion - is a non-Brownian diffusion of proteins and lipid molecules within the plasma membrane. Hop diffusion occurs ... According to the Fences and pickets model, plasma membrane is compartmentalized by actin-based membrane-skeleton "fences", ...
Actin Motor protein "Myosin". Merriam-Webster Dictionary. "myosin - definition of myosin in English from the Oxford dictionary ... rather it is a very large superfamily of genes whose protein products share the basic properties of actin binding, ATP ... F-actin, while processive runlengths are shorter on older (ADP-rich) F-actin. Myosin VI is an unconventional myosin motor, ... It walks along actin filaments, travelling towards the pointed end (- end) of the filaments. Myosin VI is thought to transport ...
F-actin capping protein. FNDC38 - Represses transcription. Tumour metastasis. Raldh2/aldh1a2 - Represses transcription. ...
protein binding. • actin binding. • receptor binding. • protein self-association. Cellular component. • cytoplasm. • cytosol. • ... actin filament. • actin cytoskeleton. • spectrin-associated cytoskeleton. • perinuclear region of cytoplasm. • endomembrane ... actin filament reorganization. • regulation of actin cytoskeleton organization. • negative regulation of peptidyl-threonine ... 1996). "Human erythrocyte dematin and protein 4.2 (pallidin) are ATP binding proteins". Biochemistry. 35 (9): 3001-6. doi: ...
Using advanced tools, protein patterns can be produced in virtually unlimited numbers (2D/ 3D shapes and volumes). ... "Geometrical and mechanical properties control actin filament organization". PLoS Comput. Biol. 11 (5): e1004245. doi:10.1371/ ... Nanopatterning of proteins has been achieved through using top-down lithography techniques. ...
In fact, annexin A-II is itself an actin-binding protein and therefore it can form a region of interaction with actin by means ... As of 2002 160 annexin proteins have been identified in 65 different species. The criteria that a protein has to meet to be ... The recognition that these proteins were members of a broad family first came from protein sequence comparisons and their cross ... Annexins can function as scaffolding proteins to anchor other proteins to the cell membrane. Annexins assemble as trimers, ...
... but autophagocytosis associated protein Atg3 and other proteins are required, and collections of thin tubules at the junction ... Actin is also believed to be important. Axe EL, Walker SA, Manifava M, Chandra P, Roderick HL, Habermann A, Griffiths G, ... Autophagy (from Greek words for "self" and "eating") is a process of digesting or degrading cytoplasmic molecules (proteins, ... There are specific receptor proteins that recruit items to the phagophore. The phagophore expands to accommodate the items, ...
After gel filtration of the actin, the actin complex, consisting of actin and its bonded proteins, began to form and the ... such as actin. Actin accounts for 5-10% of all protein found in eukaryotic cells, which therefore means that prefoldin is quite ... It resolved into five bands, therefore proving that a heterooligomeric protein is used to bind to unfolded actin. The discovery ... This solution contained an excess of cytosolic chaperonin (C-CPN), a eukaryotic chaperone protein necessary for actin folding. ...
Thin filaments are composed of protein molecules called actin. In striations of muscle bands, these are the light filaments ... I = I-band, region of just actin. H = H-zone, region of just myosin. Z = Z-line, sarcomere boundary. M = M-line, sarcomere ... Thin filaments are made of actin and attach at Z lines which help them line up correctly with each other. Troponins are ... Thick filaments are made of the elongated protein myosin. The sarcomere does not contain organelles or a nucleus. ...
Actin TES (protein) Paxillin Chen, CS; Alonso, JL; Ostuni, E; Whitesides, GM; Ingber, DE (2003). "Cell shape provides global ... Focal adhesions are integrin-containing, multi-protein structures that form mechanical links between intracellular actin ... Integrins bind to extra-cellular proteins via short amino acid sequences, such as the RGD motif (found in proteins such as ... A common metaphor to explain actin retrograde flow is a large number of people being washed downriver, and as they do so, some ...
F-actin) via direct binding to F-actin regulatory proteins. PIP2 recruits cytosolic septin monomers/oligomers to membrane ... Later studies identified associated proteins necessary during this stage, such as phosphatidylinositol transfer protein , and ... a Multifunctional Actin Regulatory Protein". The Journal of Biological Chemistry. 274 (47): 33179. doi:10.1074/jbc.274.47.33179 ... PtdIns(4,5)P2 is enriched at the plasma membrane where it is a substrate for a number of important signaling proteins. PIP2 is ...
Actin-binding LIM protein 3 is a protein that in humans is encoded by the ABLIM3 gene. The LIM domain is a double zinc finger ... ABLIM3 actin binding LIM protein family, member 3". Krupp M, Weinmann A, Galle PR, Teufel A (Jan 2006). "Actin binding LIM ... Krupp M, Weinmann A, Galle PR, Teufel A (Jan 2006). "Actin binding LIM protein 3 (abLIM3)". International Journal of Molecular ... structure that promotes protein-protein interactions. LIM domain proteins, such as ABLIM3, play roles in embryonic development ...
... is an actin bundling protein. It is a 54-58 kilodalton monomeric actin filament bundling protein originally isolated ... an actin-bundling protein essential for immunological synapse formation, and skews Fascin-1-dependent actin polarization in ... "The actin-bundling protein fascin stabilizes actin in invadopodia and potentiates protrusive invasion". Curr. Biol. 20 (4): 339 ... Fascin is a structural protein found in mesenchyme, nervous, and retinal tissue and is used in the bundling of actin molecules ...
Coronin-6 is belongs to coronin family which is an actin binding protein. Human CORO6 gene is located on chromosome 17 on the ... Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness ... Coronin-6 also known as coronin-like protein E (Clipin-E) is a protein that in humans is encoded by the CORO6 gene. ... "The coronin family of actin-associated proteins". Trends Cell Biol. 9 (9): 345-50. doi:10.1016/S0962-8924(99)01620-7. PMID ...
Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and ... "Cofilin Phosphorylation by Protein Kinase Testicular Protein Kinase 1 and Its Role in Integrin-mediated Actin Reorganization ... Maciver SK, Hussey PJ (2002). "The ADF/cofilin family: actin-remodeling proteins". Genome Biol. 3 (5): reviews3007. doi:10.1186 ... "Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase". Science. 285 (5429): 895-8. doi: ...
Ankycorbin is a protein that in humans is encoded by the RAI14 gene. Ankycorbin has been associated with the cortical actin ... a novel actin cytoskeleton-associated protein". Genes Cells. 5 (12): 1001-8. doi:10.1046/j.1365-2443.2000.00381.x. PMID ... "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3: 89. doi:10.1038/msb4100134 ... protein". Biochem. Biophys. Res. Commun. 345 (4): 1333-41. doi:10.1016/j.bbrc.2006.04.184. PMID 16729964. Ewing RM, Chu P, ...
"Entrez Gene: CORO1C coronin, actin binding protein, 1C". Human CORO1C genome location and CORO1C gene details page in the UCSC ... de Hostos EL (Oct 1999). "The coronin family of actin-associated proteins". Trends Cell Biol. 9 (9): 345-50. doi:10.1016/S0962- ... 2000). "Isolation and chromosomal assignment of a novel human gene, CORO1C, homologous to coronin-like actin-binding proteins ... Coronin-1C is a protein that in humans is encoded by the CORO1C gene. This gene encodes a member of the WD repeat protein ...
Nebulin-related-anchoring protein (N-RAP) is a protein that in humans is encoded by the NRAP gene. N-RAP is a muscle-specific ... In adult cardiac muscle, N-RAP colocalizes to intercalated discs, where it functions to anchor terminal actin filaments to the ... N-RAP is a 197 kDa protein composed of 1730 amino acids. As a member of the nebulin family of proteins, N-RAP is characterized ... "Nebulin-related-anchoring protein". Cardiac Organellar Protein Atlas Knowledgebase (COPaKB). Zong, N. C.; Li, H; Li, H; Lam, M ...
... discoideum coronin and the human p57 protein, suggesting that this protein may also be an actin-binding protein that regulates ... Coronin, actin binding protein, 2A is a protein that in humans is encoded by the CORO2A gene. This gene encodes a member of the ... This protein contains 5 WD repeats, and has a structural similarity with actin-binding proteins: the D. ... actin binding protein, 2A". Retrieved 2012-01-03. Human CORO2A genome location and CORO2A gene details page in the UCSC Genome ...
endoplasmic reticulum unfolded protein response. · protein localization to nucleus. · sterol regulatory element binding protein ... Association of emerin with nuclear and cytoplasmic actin is regulated in differentiating myoblasts. Biochem. Biophys. Res. ... It stays associated with the membrane through protein-protein interactions of itself and other membrane associated proteins, ... activation of signaling protein activity involved in unfolded protein response. · mitotic nuclear envelope disassembly. · ...
The assembly of polymerized actin with motor proteins at DNA breaks in the nucleus supports the mobility and repair of DNA. ... Actin proteins assemble to protect the genome. The assembly of polymerized actin with motor proteins at DNA breaks in the ... motor proteins called myosins walk the DNA breaks along filaments made from polymerized actin protein. b, Schrank et al.4 ... Figure 1 , Nuclear actin polymerizes to preserve genome stability. DNA is packaged with proteins in the nucleus to form a ...
We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their ... InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites ... Actin-like protein 6A (ACL6A, also known as BAF53A) is a subunit of the ATP-dependent SWI/SNF-like BAF chromatin remodelling ...
We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their ... InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites ... Novel actin-related proteins Arp-T1 and Arp-T2 as components of the cytoskeletal calyx of the mammalian sperm head.. Exp. Cell ... Arp-T1 is an actin-related protein (Arp) that serves as a component of the cytoskeletal calyx of the mammalian sperm head [PMID ...
actin-related protein 2. Names. ARP2 actin related protein 2 homolog. actin-like protein 2. ... ACTR2 actin related protein 2 [Homo sapiens] ACTR2 actin related protein 2 [Homo sapiens]. Gene ID:10097 ... evidence of a direct protein-protein interaction between PKD2 and Arp2/3 Title: Protein Interaction Network of Human Protein ... mRNA and Protein(s) * NM_001005386.2 → NP_001005386.1 actin-related protein 2 isoform a ...
Actin-binding proteins (also known as ABP) are proteins that bind to actin. This may mean ability to bind actin monomers, or ... ActA Actibind Actin Actinfilin Actinogelin Actin-regulating kinases Actin-Related Proteins Actobindin Actolinkin Actopaxin ... Many actin-binding proteins, including α-actinin, β-spectrin, dystrophin, utrophin and fimbrin, do this through the actin- ... This is a list of actin-binding proteins in alphabetical order. List: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W x Y Z ...
AFAP1 actin filament associated protein 1 [Pan troglodytes] AFAP1 actin filament associated protein 1 [Pan troglodytes]. Gene ... mRNA and Protein(s) * XM_009447333.3 → XP_009445608.2 actin filament-associated protein 1 isoform X3 ... actin filament associated protein 1provided by VGNC. Primary source. VGNC:VGNC:10814 Locus tag. CK820_G0050529. See related. ... PH1_AFAP; Actin filament associated protein family Pleckstrin homology (PH) domain, repeat 1. cd13307. Location:347 → 447. PH2_ ...
The Arf GTPase-activating protein ASAP1 regulates the actin cytoskeleton. Paul A. Randazzo, Josefa Andrade, Koichi Miura, Megan ... The Arf GTPase-activating protein ASAP1 regulates the actin cytoskeleton. Paul A. Randazzo, Josefa Andrade, Koichi Miura, Megan ... The Arf GTPase-activating protein ASAP1 regulates the actin cytoskeleton Message Subject (Your Name) has sent you a message ... The Arf GTPase-activating protein ASAP1 regulates the actin cytoskeleton. Paul A. Randazzo, Josefa Andrade, Koichi Miura, Megan ...
The Actin assembly-inducing protein (ActA) is a protein encoded and used by Listeria monocytogenes to propel itself through a ... an actin monomer binding protein, which itself promotes polymerization at barbed ends of actin filaments. Furthermore, VASP ... In the cytosol they start to polymerize actin on their surface by the help of the ActA protein. It has been shown that ActA is ... NPFs in the mammalian cell recruit and bind to the already existing actin-related-protein 2 and 3 complex (Arp2/3 complex) and ...
View protein in InterPro. IPR004000 Actin. IPR020902 Actin/actin-like_CS. IPR004001 Actin_CS. ... View protein in InterPro. IPR004000 Actin. IPR020902 Actin/actin-like_CS. IPR004001 Actin_CS. ... View protein in SMART. SM00268 ACTIN, 1 hit. PROSITEi. View protein in PROSITE. PS00406 ACTINS_1, 1 hit. PS00432 ACTINS_2, 1 ... View protein in PROSITE. PS00406 ACTINS_1, 1 hit. PS00432 ACTINS_2, 1 hit. PS01132 ACTINS_ACT_LIKE, 1 hit. ...
If you know of any papers that use this antibody, please contact us at antibodies [at] alzforum [dot] org for consideration in the References section.. ...
Actin-binding proteins (ABPs) regulate the assembly of actin filaments (F-actin) into networks and bundles that provide the ... 2000) Parallel actin bundles and their multiple actin-bundling proteins. Curr Opin Cell Biol 12:72-78.. ... 1990) Human actin-binding protein (ABP) filamin-a dystrophin-like actin-binding domain linked to 24 B-sheet repeats-ABP- ... 1981) Structure of macrophage actin-binding protein molecules in solution and interacting with actin-filaments. J Mol Biol 145: ...
Humans possess six forms of the protein actin, which perform essential functions in the body. Two in particular, β-actin and γ- ... actin, are nearly identical, only differing by four amino acids. Yet these near-twin proteins ... ... Protein structure reveals how cells regulate their skeletons. Insight into the regulation of cell skeleton structure has come ... Freely diffusing cellular proteins reach the leading edge faster. According to a theoretical model developed by LMU physicists ...
First Actin-binding Protein, circa 1975. Inverted tubes of gelled macrophage supernate (left) and water (right) for comparison ... Stossel and Hartwig had proof that the actin-binding protein initiated gelation. ... A high molecular weight polymer they would soon identify as the first actin-binding protein, later called filamin A. ... but no one understood the mechanism regulating those cytoskeleton proteins. Many researchers believed the protein contractions ...
Ab157841 is a full length protein produced in Wheat germ and has been validated in WB, ELISA. Abcam provides free protocols, ... Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Share by email ... Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all ... Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists and inhibitors. Lysates. Multiplex Assays. By ...
A process in which a protein is transported to, or maintained in, an actin cortical patch.. Synonyms. protein localisation to ... Gene Ontology Term: protein localization to actin cortical patch. GO ID. GO:0044379 Aspect. Biological Process. Description. ...
Recombinant Human beta Actin protein is a Mammalian Full length protein 1 to 375 aa range, , 85% purity and validated in SDS- ... Recombinant Human beta Actin protein. See all beta Actin proteins and peptides. ... Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all ... Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists and inhibitors. Lysates. Multiplex Assays. By ...
Actin Capping Protein. Some articles on actin, actin capping protein, protein:. Capping Protein (actin Filament) Muscle Z-line ... actin capping protein complex ... CAPZB is a member of the F-actin capping protein family ... This gene encodes the beta ... actin capping protein alpha subunit family ... This gene encodes the alpha subunit of the barbed-end actin binding protein ... ... Famous quotes containing the word protein:. "Firm-style bean curd insoles cushion feet, absorb perspiration and provide more ...
View protein in InterPro. IPR004000. Actin. IPR020902. Actin/actin-like_CS. IPR004001. Actin_CS. ... View protein in InterPro. IPR004000. Actin. IPR020902. Actin/actin-like_CS. IPR004001. Actin_CS. ... PROSITE; a protein domain and family database. More...PROSITEi. View protein in PROSITE. PS00406. ACTINS_1. 1 hit. PS01132. ... PROSITE; a protein domain and family database. More...PROSITEi. View protein in PROSITE. PS00406. ACTINS_1. 1 hit. PS01132. ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... This protein in other organisms (by gene name): P24005 - Dictyostelium discoideum 1 * Q54XA5 - Dictyostelium discoideum no ... Protein disorder predictions are based on JRONN (Troshin, P. and Barton, G. J. unpublished), a Java implementation of RONN * ... The Protein Feature View requires a browser that supports SVG (Scalable Vector Graphics). Mouse over tracks and labels for more ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... Previous Names: "actin related protein 2/3 complex, subunit 5 (16 kD)", "actin related protein 2/3 complex subunit 5, 16kDa" ... White boxes represent UTRs (untranslated regions). Orange: protein coding regions. The black lines connecting boxes represent ...
Overexpressing capping protein α and β decreases both F-actin levels and tissue growth, while expressing forms of Capping ... Protein that have dominant negative effects on F-actin promote tissue growth. Both subunits regulate each others protein ... is a master F-actin regulator. In addition to its role in many cellular processes, Capping Protein acts as a main tumor ... We show that the levels and capping activities of both subunits must be tightly regulated to control F-actin levels and ...
protein coding gene. Chr7:126699773-126707787 (-). 129S1/SvImJ MGP_129S1SvImJ_G0032994. protein coding gene. Chr7:130986959- ... protein coding gene. Chr7:140667174-140677610 (-). DBA/2J MGP_DBA2J_G0032817. protein coding gene. Chr7:125499222-125507205 (-) ... protein coding gene. Chr7:130755717-130763727 (-). BALB/cJ MGP_BALBcJ_G0032982. protein coding gene. Chr7:127179202-127187212 ... protein coding gene. Chr7:130688607-130696463 (-). C57BL/6NJ MGP_C57BL6NJ_G0033488. protein coding gene. Chr7:136088156- ...
... and protein-protein interactions and mechanism of protein folding. At the heart of all of his work, protein chemistry remains ... A Protein Condensate Drives Actin-Independent Endocytosis. Dr. Stephen Michnick(Universite de Montreal) ... Demixing of these proteins results in the formation of a protein condensate, which, by virtue of its composition and ... Although endocytosis is usually driven by F-actin polymerization in yeast cells, membrane invagination can also occur through a ...
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Compare ARP8 actin related protein 8 homolog ELISA Kits from leading suppliers on Biocompare. View specifications, prices, ... ARP8 actin related protein 8 homolog ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established antibody- ... Your search returned 19 ARP8 actin related protein 8 homolog ELISA ELISA Kit across 2 suppliers. ...
PolymerizationCortical actinMotilityCytoplasmicBranched actin filamentSkeletal MuscleFilament-associated proteinArp2DisassemblyRegulatorDynamicsEncodesMolecularStructuresEndocytosisOrganizationNucleationEukaryoticRecombinant proteinsReorganizationRegulatory proteinsInteractionFamily of proteinsAntibodyLocalizationPathwaysElongationMicrotubulesCellGelsolinConserved DomainsAminoMonomericDepolymerizationAdaptor proteinInteractionsCellsHighly conserved proteins
- Polymerization of actin also increases the mobility of DNA breaks that will be repaired by HR and the ability of a subset of DNA breaks to form clusters in the nucleus. (nature.com)
- In a mammalian cell the bacterial ActA interacts with the Arp2/3 complex and actin monomers to induce actin polymerization on the bacterial surface generating an actin comet tail. (wikipedia.org)
- Due to this conformational change, NPFs initiate polymerization of a new actin filament at a 70° angle, which leads to the characteristic Y-branched actin structures in the leading edge of motile cells. (wikipedia.org)
- Consequently, actin polymerization only starts in this region on the surface of the bacterium. (wikipedia.org)
- Continuous actin polymerization is sufficient for motility in the cytoplasm and even for infection of adjacent cells. (wikipedia.org)
- Although endocytosis is usually driven by F-actin polymerization in yeast cells, membrane invagination can also occur through a yet unknown actin-independent mechanism when turgor pressure is relieved. (concordia.ca)
- To elucidate the underlying molecular mechanisms, we undertook a domain analysis of DRR1 and probed the effects on actin binding, polymerization, and bundling, as well as on actin-dependent cellular processes. (mdpi.com)
- Methods: DRR1 domains were cloned and expressed as recombinant proteins to perform in vitro analysis of actin dynamics (binding, bundling, polymerization, and nucleation). (mdpi.com)
- Finally, twinfilin 2, a putative modulator of actin polymerization, was found at the tips of stereocilia. (mit.edu)
- Profilin - A protein with actin binding activity thus inhibiting actin polymerization. (tebu-bio.com)
- Cofilin - An ABP that binds and depolymerizes filamentous F-actin while inhibiting the polymerization of G-actin. (tebu-bio.com)
- Excessive actin filament polymerization is not sufficient to explain this phenotype, as loss of Cofilin or Cyclase-associated protein does not cause cell extrusion or death. (biologists.org)
- Here, we report that the actin-Capping Protein αβ heterodimer, which regulates actin polymerization, also functions to suppress inappropriate tissue growth by inhibiting Yorkie activity. (biologists.org)
- A large number of proteins sit at the interface between actin networks and membranes, playing diverse roles such as initiation of actin polymerization, modulation of membrane curvature, and signaling. (springer.com)
- Abou-Kheir W, Isaac B, Yamaguchi H, Cox D (2008) Membrane targeting of WAVE2 is not sufficient for WAVE2-dependent actin polymerization: a role for IRSp53 in mediating the interaction between Rac and WAVE2. (springer.com)
- Almeida-Souza L et al (2018) A flat BAR protein promotes actin polymerization at the base of clathrin-coated pits. (springer.com)
- Studying actin polymerization in vitro . (cytoskeleton.com)
- Studying the effects of actin binding proteins and drugs on actin polymerization in vitro . (cytoskeleton.com)
- Fluorescence enhancement during pyrene actin polymerization. (cytoskeleton.com)
- Pyrene muscle actin was polymerized in duplicate wells of a 96-well plate by the addition of Actin Polymerization Buffer (Cat. (cytoskeleton.com)
- Consequently, suppressing PLEKHG2 expression blocked actin polymerization and SDF1α-stimulated lymphocyte migration. (asm.org)
- Together, our studies have provided the first evidence for the endogenous function of PLEKHG2, which may serve as a key Gβγ-stimulated RhoGEF that regulates lymphocyte chemotaxis via Rac and Cdc42 activation and actin polymerization. (asm.org)
- WASP/WAVE family proteins act as a switch to activate actin polymerization and five members of the family are known: WASP, N-WASP, WAVE1, WAVE2, and WAVE3. (aacrjournals.org)
- In the present paper we have documented the binding of 50K-A to DNAase I and the effect of 50K-A on the kinetics of actin polymerization. (biochemj.org)
- When 50K-A was added to pyrene-labelled rabbit skeletal-muscle actin and the salt concentration increased, the initial rate of actin polymerization was inhibited by a very low molar ratio of 50K-A to actin. (biochemj.org)
- Unlike full-length talin, the C-terminal f-actin binding domain is unable to nucleate actin polymerization. (ovid.com)
- Evidence is presented here that fesselin accelerates the polymerization of actin. (uncg.edu)
- Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. (biomol.com)
- In unicellular eukaryotes such as budding yeast, disruption of ARP2/3 complex subunits results in a slow-growth phenotype characterized by defects in cortical actin patches. (plantcell.org)
- Immunofluorescence staining revealed that the Pan1 protein colocalized with the cortical actin patches, suggesting that it may be a filamentous actin-binding protein. (asm.org)
- In cultured podocytes Schip1 localizes to cortical actin-rich regions of lamellipodia, where it forms a complex with Nherf2 and ezrin, proteins known to participate in actin remodeling stimulated by PDGF beta signaling. (diva-portal.org)
- A network of cortical actin is also strengthened during this stage. (uiowa.edu)
- Additionally, Fascin is required for strengthening of the cortical actin network downstream of PGs. (uiowa.edu)
- This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. (nih.gov)
- Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. (abcam.com)
- Capping contributes to the control of actin-based motility in non-muscle cells. (atlasgeneticsoncology.org)
- Dysregulation of actin-based motility is a prominent factor in cell transformation and is probably associated with carcinogenesis. (atlasgeneticsoncology.org)
- High level of CapG protein may possibly play an important role in pancreatic cancer cell motility and consequently dissemination. (atlasgeneticsoncology.org)
- A gene on chromosome 11q13.2 that encodes an actin-binding protein which regulates leading edge dynamics and cell motility in fibroblasts, and may be involved in cytokinesis and signal transduction. (thefreedictionary.com)
- Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. (abbkine.com)
- Actins are generally important in the area of cell structure, motility, and integrity, and β-actin constitutes a major part of the contractile apparatus used as a loading control. (abbkine.com)
- The folding of actin and tubulin is mediated via interaction with a heteromeric toroidal complex (cytoplasmic chaperonin) that hydrolyzes ATP as part of the reaction whereby native proteins are ultimately released. (rupress.org)
- In each case, the unfolded polypeptide forms a binary complex with cytoplasmic chaperonin and is released as a soluble, monomeric protein in the presence of Mg-ATP and the presence or absence of Mg-GTP. (rupress.org)
- We show that actin-RPV and gamma-tubulin compete with one another, and with beta-actin or alpha-tubulin, for binary complex formation with cytoplasmic chaperonin. (rupress.org)
- Immunohistochemistry-Paraffin: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-- Staining of human lymph node shows strong cytoplasmic positivity in lymphoid cells outside reaction centra. (novusbio.com)
- Here, by cloning and analyzing the promoter sequence of the gene encoding cytoplasmic actin from the leafhopper Nephotettix cincticeps, we successfully developed the first efficient transient expression vector for cultured leafhopper cells, which can also be used to express exogenous proteins in other insect culture cell lines, including those derived from Recilia dorsalis leafhopper and Spodoptera frugiperda (Sf9). (frontiersin.org)
- genetic loss of Fascin or the Drosophila COX-like enzyme Pxt (Peroxinectin-like) leads to disruption of cytoplasmic actin remodeling, and ultimately, female sterility. (uiowa.edu)
- A recent study showed that CP is present in dendritic spines of cultured hippocampal neurons and the branched actin filament network containing CP appears to be a prominent feature of the spine head ( Korobova and Svitkina, 2010 ). (jneurosci.org)
- Actin's role in these processes is tightly regulated, in particular through the Arp2/3 complex ( Goley and Welch, 2006 ), a major initiator of actin polymerisation that promotes the formation of branched actin-filament networks. (biologists.org)
- Lamellipodia are wide but thin in cross section, and this characteristic morphology is due to the construction of branched actin filament arrays within them ( 3 , 4 ). (aacrjournals.org)
- Actin Filament Associated Protein 1 (AFAP1) is a Src binding partner. (prospecbio.com)
- Human XB130 was discovered in Dr. Mingyao Liu's laboratory (University of Toronto) in the process of cloning human actin filament associated protein (afap) gene. (atlasgeneticsoncology.org)
- XB130 is a novel adaptor protein, member of the actin filament associated protein (AFAP) family (Snyder et al. (atlasgeneticsoncology.org)
- An important finding of Caridi and colleagues is that recruitment of Arp2/3 and myosins at heterochromatic DSBs requires the DSB-repair protein Mre11 and a heterochromatin component, the HP1α protein. (nature.com)
- however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. (nih.gov)
- Actin-Related Protein 2 (ARP2) and Virus-Induced Filopodia Facilitate Human Respiratory Syncytial Virus Spread. (nih.gov)
- Actin-related protein (Arp2) inserts into artificial lipid membranes. (nih.gov)
- Proteins involved in actin-filament-based processes, specifically CDC42 and members of the ARP2/3 complex are crucial for establishment of Japanese encephalitis virus infection. (nih.gov)
- NPFs in the mammalian cell recruit and bind to the already existing actin-related-protein 2 and 3 complex (Arp2/3 complex) and induce an activating conformational change of the Arp2/3 complex. (wikipedia.org)
- ACTIN-RELATED PROTEINS 2 and 3 form the major subunits of the ARP2/3 complex, which is known as an important regulator of actin organization in diverse organisms. (plantcell.org)
- In mammalian cells, the actin-related protein 2 and 3 (Arp2/3) complex initiates actin assembly to form lamellipodial protrusions by binding to Wiskott-Aldrich syndrome (WASP)/WASP family verproline-homologous protein 2 (WAVE2). (aacrjournals.org)
- Actin-related protein 2 and 3 complex (Arp2/3 complex) and proteins of the Wiskott-Aldrich syndrome (WASP)/WASP family verproline-homologous protein (WAVE) family are involved in the process of lamellipodium formation. (aacrjournals.org)
- Functions as component of the Arp2/3 complex which is involved in regulation of actin. (biomol.com)
- Furthermore, actin stress fiber disassembly was significantly more pronounced in cells infected with parental EHV-1, revertant, or the recombinant EHV-1 expressing EHV-4 US3 . (mdpi.com)
- Actin filament growth, stability and disassembly are controlled by a plethora of actin-binding proteins. (biologists.org)
- 9 ) showed that a viral protein secreted into the medium of infected Caco-2 cells likewise causes disassembly of microvillar actin in noninfected cells. (asm.org)
- In contrast with the Ca2+-dependent binding to DNAase, the effect of 50K-A on the kinetics of actin assembly and disassembly was Ca2+-independent. (biochemj.org)
- The actin-Capping Protein heterodimer, composed of the α and β subunits, is a master F-actin regulator. (plos.org)
- Here, we report that actin capping protein (CP), a regulator of actin filament growth, plays an essential role for spine development and synapse formation. (jneurosci.org)
- Nebulin, a putative F-actin regulator, was located toward the base of stereocilia. (mit.edu)
- At the heart of all of his work, protein chemistry remains central, but he has developed an eclectic and diverse research program aimed at studying the organization and dynamics of biochemical networks in living cells and how these networks compute cell-fate decisions. (concordia.ca)
- Downregulated in renal cell carcinoma 1 (DRR1) protein was characterized as the link between stress, actin dynamics, neuronal function, and cognition. (mdpi.com)
- A tight spatio-temporal coordination of the machineries controlling actin dynamics and membrane remodelling is crucial for a huge variety of cellular processes that shape cells into a multicellular organism. (pubmedcentralcanada.ca)
- The BAR/F-BAR-domain-containing proteins are prime candidates to couple plasma membrane curvature and actin dynamics in different morphogenetic processes. (pubmedcentralcanada.ca)
- Over the past years, the Bin/Amphiphysin/Rvs (BAR) proteins have emerged as prime candidates to couple actin dynamics and membrane trafficking. (pubmedcentralcanada.ca)
- Several such proteins, like a subset of BAR domain proteins, bind to dynamin, a GTPase implicated in endocytosis and actin dynamics, via SH3 domains. (nih.gov)
- These results suggest a close interplay between the mechanisms that control actin dynamics and those that mediate plasma membrane invagination and fission. (nih.gov)
- Quantitative analyses of actin dynamics in live cells have uncovered the potential molecular mechanisms that contribute to increased filament abundance. (plantphysiol.org)
- Taken together, these findings indicate a novel interplay between Hippo pathway activity and actin filament dynamics that is essential for normal growth control. (biologists.org)
- These observations suggest a role for F-actin dynamics in modulating Hpo pathway activity. (biologists.org)
- We have also shown that the DC migratory defect is cell autonomous and that Eps8 is required for the proper architectural organization of the actin meshwork and dynamics of cell protrusions. (pubmedcentralcanada.ca)
- Within this context, regulators of actin dynamics, including actin bundlers and capping proteins, are expected to play a critical role by controlling the architecture and dynamics of the actin meshwork that propels nonadhesive DC migration. (pubmedcentralcanada.ca)
- The STICS technique reveals protein dynamics even when protein densities are very low or very high, and works in the presence of large, static molecular complexes. (biologists.org)
- Because all of these ABP/actin structures are located along force-transmission pathways, it is important to understand how forces affect the molecular interactions and the role that ABPs play in sustaining and regulating the mechanical behavior of the cell. (pnas.org)
- Instead, their test tube was filled with some "contaminant": A high molecular weight polymer they would soon identify as the first actin-binding protein, later called filamin A. (the-scientist.com)
- ActA has a molecular weight of 70,349 Da and is a surface protein. (wikipedia.org)
- Western blot shows the protein molecular weight is 130 kD (Xu et al. (atlasgeneticsoncology.org)
- The full length protein consists of 818 amino acids with a molecular weight of approximately 130 kDa by western blotting (Xu et al. (atlasgeneticsoncology.org)
- The purification method used (according to Spudich & Watts) results in a highly purified protein having a Molecular mass of 43,000 dalton. (prospecbio.com)
- Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)
- Here, we demonstrate that rapid actin remodeling upon elicitation with diverse microbe-associated molecular patterns and damage-associated molecular patterns represent a conserved plant immune response. (plantphysiol.org)
- More broadly though, this structure presents a picture of the intimate interactions between the subunits in the actinin dimer, providing a framework to better understand the molecular details of actin cross-linking and its regulation in all actinins and perhaps in spectrins too. (springer.com)
- Pyrene labeled rabbit muscle actin has an approximate molecular weight of 43 kDa, and is supplied as a white lyophilized powder. (cytoskeleton.com)
- A 100 µg sample of pyrene muscle actin (molecular weight approx. (cytoskeleton.com)
- In VP22-null virions, three proteins of apparent molecular masses of 28, 46, and 53 kDa increased relative to other virion proteins (Fig. 1 , right arrowheads). (asm.org)
- In vivo , the organization of F-actin into higher-order structures is regulated by a wide variety of actin-binding proteins (ABPs) ( 2 - 8 ). (pnas.org)
- Although the amino acid sequences of the α and β subunits are not more similar to each other than they are to other ABPs, nor they share common sequences with other proteins, they have extremely similar secondary and tertiary structures . (plos.org)
- Prof. Michnick trained principally as a biophysical chemist, studying structures and binding of complex cell surface carbohydrate- and protein-protein interactions and mechanism of protein folding. (concordia.ca)
- In living cells, actin structures can be observed by incorporating fluorescently labeled actin, by expressing GFP-actin, or by expressing a fluorescently labeled actin binding protein sub-domain. (cytoskeleton.com)
- Fluorescent actin is the most accurate reporter of actin structures. (cytoskeleton.com)
- To perform these functions, DCs flexibly adapt their adhesive, actin-based structures and migratory properties. (pubmedcentralcanada.ca)
- Dexamethasone treatment led to redistribution of Hic-5 predominantly to the focal points of cross linked actin network (CLAN) like structures. (arvojournals.org)
- Fascin is most commonly associated with the formation of filopodia and invadapodia (parallel actin filament bundle structures) to drive migration and invasion. (uiowa.edu)
- These actin structures are essential for the completion of oogenesis, and ultimately female fertility. (uiowa.edu)
- 1 - 3 The actin filament system also contributes to development by regulating endocytosis, a fundamental process that is essential to downregulate or attenuate extracellular signaling, to shape morphogen gradients and to orchestrate cell fate decisions within a tissue. (pubmedcentralcanada.ca)
- Cisplatin resistant (CP-r) cells often show decreased uptake of cisplatin in association with reduced cell surface proteins and decreased endocytosis. (aspetjournals.org)
- Actin is found in all eukaryotic cells (except for nematode sperm). (prospecbio.com)
- Thus, they were a component of early eukaryotic actin-based cytoskeletons, though notably, they are not found in plants. (springer.com)
- Giardia intestinalis, a human intestinal parasite and member of what is perhaps the earliest-diverging eukaryotic lineage, contains the most divergent eukaryotic actin identified to date and is the first eukaryote known to lack all canonical actin-binding proteins (ABPs). (washington.edu)
- Custom ELISA Kits, Recombinant Proteins and Antibodies can be designed, manufactured and produced according to the researcher's specifications. (mybiosource.com)
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- Proft A, Spiesschaert B, Izume S, Taferner S, Lehmann MJ, Azab W. The Role of the Equine Herpesvirus Type 1 (EHV-1) US3-Encoded Protein Kinase in Actin Reorganization and Nuclear Egress. (mdpi.com)
- Each of these treatments attenuated actin reorganization induced by flow. (ahajournals.org)
- Deficiency of Hic-5 led to loss of TGFβ2 and Dexamethasone- induced reorganization of actin as well as significant decreases in protein levels of myocilin, α-SMA and collagen 1A in TM cells. (arvojournals.org)
- Thus, CP appears to cap F-actin barbed ends via the independent interaction of both tentacles with actin. (plos.org)
- Although recent results indicate that Amp is involved in vector specificity interacting with insect proteins such as actin, little is known about the interaction of IDP with the plant. (apsnet.org)
- Here we show that actin-RPV and gamma-tubulin are both folded via interaction with the same chaperonin that mediates the folding of beta-actin and alpha- and beta-tubulin. (rupress.org)
- The WH1 domain interacts with the WASP-interacting protein (WIP) family of proteins, and this interaction is thought to suppress the activity of WASP or N-WASP. (biologists.org)
- WASP and N-WASP are predominantly found in an autoinhibited conformation in which the C-terminus of the protein is occluded through its interaction with the N-terminus. (biologists.org)
- The interaction of ERK with actin raises the possibility that actin may provide a scaffold for ERK signalling complexes in both muscle and non-muscle cells. (biochemj.org)
- The BTB/POZ domain: a new protein-protein interaction motif common to DNA- and actin-binding proteins -- Albagli et al. (aacrjournals.org)
- The BTB/POZ domain defines a newly characterized protein-protein interaction interface. (aacrjournals.org)
- Saccharomyces cerevisiae Abp1p is a member of the Abp1 family of proteins, which are in diverse organisms including fungi, nematodes, flies and mammals. (genetics.org)
- The BAR domain family of proteins comprises a diverse group of multi-functional effectors, characterized by their modular architecture. (springer.com)
- Determination of the actin-binding domain of the EGFR was done by measuring competition of either a polyclonal antibody or synthetic peptides on EGFR cosedimentation with F-actin. (rupress.org)
- A polyclonal antibody raised against HL-33 was found to prevent cosedimentation of EGFR with F-actin. (rupress.org)
- Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins. (novusbio.com)
- Fig.2.Western blot analysis (1:5000) of β-Actin expression in Rat brain (lane 1), HeLa cell lysate (lane 2), Mouse brain (lane 3), Chicken lung (lane 4) and Rabbit testis (lane 5) with β-actin Polyclonal Antibody. (abbkine.com)
- The antibody detects endogenous β-actin protein. (abbkine.com)
- Protein expression was analyzed by immunoblotting 20 μg of total cell lysate (TCL) with an anti-GFP antibody (left panel). (elifesciences.org)
- Phosphorylated proteins were purified from TCL with Phos-tag agarose, run on SDS-PAGE and immunoblotted with the anti-GFP antibody (right panel) as described in Materials and methods. (elifesciences.org)
- If you cannot find the target and/or product is not available in our catalog, please click here to contact us and request the product or submit your request for custom elisa kit production , custom recombinant protein production or custom antibody production . (mybiosource.com)
- Abbkine Scientific Company Limited recently announced the release of a new product called Anti-β-Actin Mouse Monoclonal Antibody (1C7), a synthetic peptide hosted by the mouse. (abbkine.com)
- Using isoform-specific antibodies and probes for in situ hybridization, we observed distinct localization patterns for β- and γ-actin within cultured cerebrocortical neurons. (jneurosci.org)
- Results: We have constructed fusion proteins consisting of green fluorescent protein (GFP) with either the entire cross-linking protein or its actin-binding domain (ABD) and examined the localization of these fluorescent proteins in living cells under a variety of conditions. (harvard.edu)
- Surprisingly, the localization of the GFP-actin-binding domain fusion proteins precisely reflected that of their respective full length constructs, indicating that the localization of the protein was determined by the actin-binding domain alone. (harvard.edu)
- At the nuclear periphery, Fascin localization requires components of the protein complex that links the nucleoplasm to the cytoplasm, termed the LINC complex. (uiowa.edu)
- In order to determine the function of twinfilin 2, I localized the protein predominately to the tips of shorter stereocilia where it is up-regulated during the final phase of elongation. (mit.edu)
- Fesselin did not alter the critical concentration for actin but did increase the rate of elongation by â‰ˆ3-fold. (uncg.edu)
- Reports that actin also has functions in the cell nucleus remain controversial 1 , 2 , partly because of the challenges of performing experiments that exclusively perturb the nuclear actin pool without also perturbing actin in the cytoplasm. (nature.com)
- Two studies 3 , 4 in Nature now provide the most compelling evidence so far that polymerized actin has roles in cell nuclei in which DNA has been damaged, and that it could be essential for maintaining genome stability. (nature.com)
- In cell nuclei, DNA is packaged with proteins to form a material called chromatin, which is subdivided into different domains. (nature.com)
- On the other hand, ASAP1, with a mutation that disrupted GTPase-activating protein activity, had a reduced effect on cell spreading and increased the number of cells forming dorsal ruffles in response to PDGF. (pnas.org)
- Conversely, bundling proteins, including α-actinin, form thick F-actin cables that help both in maintaining the cell under a prestressed state ( 1 , 18 ) and generating protrusions at the leading edge of a migrating cell ( 11 , 19 ). (pnas.org)
- According to a theoretical model developed by LMU physicists, in cell protrusions, cargo-transporting motor proteins often get in each other's way. (phys.org)
- Though initially a controversial discovery, filamin A proved to be the first of hundreds of binding proteins that influence cell movement. (the-scientist.com)
- All mutants except the actA mutants were similar to wild-type concerning association with F-actin and cell-cell spreading. (wikipedia.org)
- Nesprin-1 and nesprin-2 (also known as Syne-1 and Syne-2,) are large ( approximately 3300-residue) vertebrate proteins associated with emerin and lamin A at the nuclear envelope of muscle cells and other cell types. (nih.gov)
- Significant overexpression of CapG protein was detected in oral squamous-cell carcinoma cells (OSCCs) and oral premalignant lesions (OPLs). (atlasgeneticsoncology.org)
- Expression of the CapG protein was lost in the tumorigenic cell line, isolated from a human diploid fibroblast lineage. (atlasgeneticsoncology.org)
- The CapG protein expression was lost also in cancer cell lines including stomach cancer , lung cancer and melanoma . (atlasgeneticsoncology.org)
- β-Actin protein was highly enriched within growth cones and filopodia, in contrast to γ-actin protein, which was distributed uniformly throughout the cell. (jneurosci.org)
- β-Actin mRNAs were localized more frequently to neuronal processes and growth cones, unlike γ-actin mRNAs, which were restricted to the cell body. (jneurosci.org)
- A major challenge of neuronal cell biology is to identify how the protein composition of the growth cone differs from the perikarya and to identify mechanisms involved in this sorting and assembly. (jneurosci.org)
- The availability of mRNAs within processes and growth cones could allow the neuron to circumvent the need to transport newly synthesized proteins from the cell body, thus providing the growth cone with autonomous control of its own structure. (jneurosci.org)
- These developmental processes can be regulated through actin dependent vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. (harvard.edu)
- Palladin plays important roles in the regulation of diverse actin-related signaling in a number of cell types. (osti.gov)
- However, it is involved in the regulation of actin-based cell migration in non-muscle benign cells ( 19 ). (spandidos-publications.com)
- In Drosophila , mutations in either cpa or cpb , lead to accumulation of F-actin within the cell and give rise to identical developmental phenotypes that are tissue specific. (biologists.org)
- Actinins are one of the major actin cross-linking proteins found in virtually all cell types and are the ancestral proteins of a larger family that includes spectrin, dystrophin and utrophin. (springer.com)
- In this study, we show that miR-206 was decreased in TNBC clinical tumor samples and cell lines whereas one of its predicted targets, actin-binding protein CORO1C, was increased. (aacrjournals.org)
- Further, silencing of CORO1C reduced tumor cell migration and affected the actin skeleton and cell morphology, similar to miR-206 expression, but did not reduce proliferation. (aacrjournals.org)
- However, the very large number of proteins involved in this linkage and their complex network of interactions make it difficult to assess their role in cell migration. (biologists.org)
- The data show that there are differences in the efficiency of the linkage between integrin and actin among different cell types and on the same cell type grown on different substrate densities. (biologists.org)
- In polarized human intestinal Caco-2 cells, rotavirus infection induces calcium-dependent depolymerization of microvillar actin and interferes with protein trafficking ( 9 , 27 ) and cell monolayer integrity by disruption of tight junctions ( 39 ). (asm.org)
- To minimize the cytotoxicity of NSP4 expression ( 45 ), we used an inducible HEK 293-derived cell line that expresses NSP4 fused to enhanced green fluorescent protein (EGFP) upon activation of a tetracycline (Tet)-responsive promoter ( 5 , 6 ). (asm.org)
- The activity of RhoGEFs is often regulated by cell surface receptors, such as growth factor receptors and G protein-coupled receptors (GPCRs). (asm.org)
- At the level of individual cell, several different WASP/WAVE family proteins are expressed together in the cytoplasm and they are thought to cooperate in cell migration by forming protrusions ( 1 , 15 - 18 ). (aacrjournals.org)
- Decreased expression of these two proteins was found in two different human CP-r cell lines (KB-CP20 and 7404-CP20), in comparison with their parental cell lines (KB-3-1 and BEL-7404), respectively. (aspetjournals.org)
- During stage 10B, an array of radially-aligned actin filament bundles form at the nurse cell membranes and extend inwards towards the nucleus. (uiowa.edu)
- Hearing and vestibular function depend on mechanosensory staircase collections of hair cell stereocilia, which are produced from microvillus-like precursors as their parallel actin bundle scaffolds increase in diameter and elongate or shorten. (prolekare.cz)
- Hair cell stereocilia contain multiple classes of actin-bundling protein, but little is known about what each class contributes. (prolekare.cz)
- Your search returned 5 capping protein (actin filament), gelsolin-like ELISA ELISA Kit across 2 suppliers. (biocompare.com)
- Gelsolin - an ABP controlling actin filament length. (tebu-bio.com)
- In the present study, we identified a vital oncoprotein, capping actin protein, gelsolin-like (CapG), and investigated its roles in the prognosis, proliferation and metastasis in glioma. (spandidos-publications.com)
- Capping actin protein, gelsolin-like (CapG) was first identified as an actin binding protein of the gelsolin superfamily, which is able to modulate actin length by capping barbed ends in a Ca 2+ -dependent manner and generate propulsive force ( 17 , 18 ). (spandidos-publications.com)
- These results suggest that 50K-A is a novel actin-binding protein with some similarities to the severin/fragmin/gelsolin family of F-actin-capping proteins. (biochemj.org)
- The duplicated domain is highly homologous to three other actin-severing proteins and this basic structure represents the whole molecule in severin and fragmin, while two basic units compose gelsolin. (pasteur.fr)
- Two in particular, β-actin and γ-actin, are nearly identical, only differing by four amino acids. (phys.org)
- Therefore, the sequence of the mature ActA protein consist of 610 amino acids. (wikipedia.org)
- We show that the previously described nesprins are short isoforms of giant proteins comprising an actin-binding amino-terminus connected to a carboxy-terminal klarsicht-related transmembrane domain by a massive ( approximately 6000-8000 amino acid) spectrin-like rod domain, making full-length nesprin-1, at one megadalton, the largest non-titin protein hitherto described in humans. (nih.gov)
- The C-terminus of mouse talin (amino acids 2345-2541) is responsible for all of the protein's f-actin binding capacity. (ovid.com)
- ACTL7B: is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. (mybiosource.com)
- I hope this review will help you gain a better view of ABPs and actin interactions. (tebu-bio.com)
- These observations lead to a hypothesis in which the linkage between the ECM and actin is regulated by changes in interactions among linkage components through the action or modification by signaling molecules such as Src, paxillin and FAK. (biologists.org)
- 4 report that, in human cells, polymerized actin promotes the processing (resection) of broken DNA ends in chromatin (the compartments involved were not determined) and facilitates a DNA-repair pathway called homologous recombination (HR, not shown), although the underlying mechanism is unclear. (nature.com)
- In this paper, we show that ASAP1 localizes to focal adhesions and cycles with focal adhesion proteins when cells are stimulated to move. (pnas.org)
- Many researchers believed the protein contractions were controlled by calcium, similar to muscle cells. (the-scientist.com)
- The pair hypothesized that their protein was responsible for the transformation of cytoplasm from a liquid to gel that occurs as cells move and engulf food, and they resolved to prove it. (the-scientist.com)
- These mutant bacteria still escaped from the phagosomes as efficiently as wild-type bacteria and multiplied within the infected cells but they were not surrounded by actin like wild-type bacteria. (wikipedia.org)
- Furthermore, CapG protein is also transported more rapidly into the nucleus of the breast cancerous cells than the corresponding benign cells. (atlasgeneticsoncology.org)
- The full-length fusion proteins, but not the ABD's complemented the defects of cells lacking both endogenous proteins indicating that they are functional. (harvard.edu)
- Interestingly, F-actin also accumulates abnormally when Hippo pathway activity is reduced or abolished, independently of Yorkie activity, whereas overexpression of the Hippo pathway component expanded can partially reverse the abnormal accumulation of F-actin in cells depleted for Capping Protein. (biologists.org)
- We apply a novel image analysis tool, spatio-temporal image correlation spectroscopy (STICS), to quantify the directed movements of adhesion-related proteins and actin in protrusions of migrating cells. (biologists.org)
- Despite its importance, few studies have addressed the ECM-actin linkage and its regulation in migrating cells. (biologists.org)
- Immunoelectron microscopy localized ALP1 to a discrete region of the nuclear envelope, on transport vesicles, and on the nascent IMC of the daughter cells prior to the arrival of proteins such as IMC-1. (asm.org)
- PRV Us2 protein is prenylated in infected cells, but the nonprenylated form is packaged in the tegument of virions ( 8 ). (asm.org)
- Disorganization of β-actin and filamin 250 and 90 was also detected in these CP-r cells by confocal microscopy. (aspetjournals.org)
- Arginine pancreatitis was accompanied by a stress response with a large increase in the small heat shock protein HSP27, as well as HSP70, peaking at 24 hours and localised to acinar cells. (bmj.com)
- D ) Alexa Fluor 488-phalloidin staining of fixed wild-type and pan1-18TA cells to visualize actin. (elifesciences.org)
- Wild-type, sla1-10TA , and pan1-18TA sla1-10TA cells were fixed and stained with Alexa Fluor 488-phalloidin to visualize actin. (elifesciences.org)
- Expression of recombinant Hic-5 in HTM cells induced bundling and crosslinking of actin filament and caused significant increase in the levels of myocilin and α-SMA and collagen 1A relative to GFP expressing control cells. (arvojournals.org)
- The nurse cells are of particular interest because they are the sites of dynamic actin remodeling during mid-late oogenesis. (uiowa.edu)
- Numerous studies, both in living cells and in reconstituted actin gels, have been conducted, but important questions still remain. (asme.org)