Actins
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
Actin Cytoskeleton
Actin Depolymerizing Factors
Actin Capping Proteins
Cytoskeleton
Phalloidine
Gelsolin
Profilins
Cytochalasin D
A fungal metabolite that blocks cytoplasmic cleavage by blocking formation of contractile microfilament structures resulting in multinucleated cell formation, reversible inhibition of cell movement, and the induction of cellular extrusion. Additional reported effects include the inhibition of actin polymerization, DNA synthesis, sperm motility, glucose transport, thyroid secretion, and growth hormone release.
Myosins
A diverse superfamily of proteins that function as translocating proteins. They share the common characteristics of being able to bind ACTINS and hydrolyze MgATP. Myosins generally consist of heavy chains which are involved in locomotion, and light chains which are involved in regulation. Within the structure of myosin heavy chain are three domains: the head, the neck and the tail. The head region of the heavy chain contains the actin binding domain and MgATPase domain which provides energy for locomotion. The neck region is involved in binding the light-chains. The tail region provides the anchoring point that maintains the position of the heavy chain. The superfamily of myosins is organized into structural classes based upon the type and arrangement of the subunits they contain.
Bicyclo Compounds, Heterocyclic
Actin-Related Protein 2-3 Complex
Contractile Proteins
Pseudopodia
Tropomyosin
Cytoskeletal Proteins
Actin-Related Protein 2
Actin-Related Protein 3
Rabbits
Protein Binding
Polymerization
Actomyosin
Microscopy, Fluorescence
Actinin
Polymers
Depsipeptides
Wiskott-Aldrich Syndrome Protein, Neuronal
Biopolymers
Wiskott-Aldrich Syndrome Protein
rho GTP-Binding Proteins
Cytochalasins
Cell Movement
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
cdc42 GTP-Binding Protein
Myosin Type II
Cells, Cultured
Amino Acid Sequence
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Filamins
A family of crosslinking filament proteins encoded by distinct FLN genes. Filamins are involved in cell adhesion, spreading, and migration, acting as scaffolds for over 90 binding partners including channels, receptors, intracellular signaling molecules and transcription factors. Due to the range of molecular interactions, mutations in FLN genes result in anomalies with moderate to lethal consequences.
Vinculin
Wiskott-Aldrich Syndrome Protein Family
A family of microfilament proteins whose name derives from the fact that mutations in members of this protein family have been associated with WISKOTT-ALDRICH SYNDROME. They are involved in ACTIN polymerization and contain a polyproline-rich region that binds to PROFILIN, and a verprolin homology domain that binds G-ACTIN.
Viscosity
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Models, Biological
Chickens
Carrier Proteins
Dictyostelium
Cell Surface Extensions
Microtubules
rhoA GTP-Binding Protein
Cell Membrane
Signal Transduction
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Calmodulin-Binding Proteins
rac1 GTP-Binding Protein
A rac GTP-binding protein involved in regulating actin filaments at the plasma membrane. It controls the development of filopodia and lamellipodia in cells and thereby influences cellular motility and adhesion. It is also involved in activation of NADPH OXIDASE. This enzyme was formerly listed as EC 3.6.1.47.
rac GTP-Binding Proteins
Green Fluorescent Proteins
Phosphorylation
Binding Sites
Fibroblasts
Recombinant Fusion Proteins
Myosin Type I
Protein Transport
Mutation
Microscopy, Confocal
Myosin Type V
Adenosine Triphosphate
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Tropomodulin
Focal Adhesions
An anchoring junction of the cell to a non-cellular substrate. It is composed of a specialized area of the plasma membrane where bundles of the ACTIN CYTOSKELETON terminate and attach to the transmembrane linkers, INTEGRINS, which in turn attach through their extracellular domains to EXTRACELLULAR MATRIX PROTEINS.
Macromolecular Substances
Calcium
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Endocytosis
Muscle Proteins
Phosphatidylinositol 4,5-Diphosphate
A phosphoinositide present in all eukaryotic cells, particularly in the plasma membrane. It is the major substrate for receptor-stimulated phosphoinositidase C, with the consequent formation of inositol 1,4,5-triphosphate and diacylglycerol, and probably also for receptor-stimulated inositol phospholipid 3-kinase. (Kendrew, The Encyclopedia of Molecular Biology, 1994)
Cytoplasm
rho-Associated Kinases
A group of intracellular-signaling serine threonine kinases that bind to RHO GTP-BINDING PROTEINS. They were originally found to mediate the effects of rhoA GTP-BINDING PROTEIN on the formation of STRESS FIBERS and FOCAL ADHESIONS. Rho-associated kinases have specificity for a variety of substrates including MYOSIN-LIGHT-CHAIN PHOSPHATASE and LIM KINASES.
Molecular Motor Proteins
Muscle, Smooth
Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed)
Amoeba
Models, Molecular
Microscopy, Video
Membrane Proteins
Saccharomyces cerevisiae
Cross-Linking Reagents
Reagents with two reactive groups, usually at opposite ends of the molecule, that are capable of reacting with and thereby forming bridges between side chains of amino acids in proteins; the locations of naturally reactive areas within proteins can thereby be identified; may also be used for other macromolecules, like glycoproteins, nucleic acids, or other.
Calcium-Binding Proteins
Protein Conformation
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Electrophoresis, Polyacrylamide Gel
Rhodamines
Adenosine Diphosphate
Protein Isoforms
Myosin Heavy Chains
Myofibrils
Zyxin
Cytoplasmic Streaming
Muscle, Skeletal
Adenosine Triphosphatases
Botulinum Toxins
Toxic proteins produced from the species CLOSTRIDIUM BOTULINUM. The toxins are synthesized as a single peptide chain which is processed into a mature protein consisting of a heavy chain and light chain joined via a disulfide bond. The botulinum toxin light chain is a zinc-dependent protease which is released from the heavy chain upon ENDOCYTOSIS into PRESYNAPTIC NERVE ENDINGS. Once inside the cell the botulinum toxin light chain cleaves specific SNARE proteins which are essential for secretion of ACETYLCHOLINE by SYNAPTIC VESICLES. This inhibition of acetylcholine release results in muscular PARALYSIS.
Saccharomyces cerevisiae Proteins
Myosin Light Chains
The smaller subunits of MYOSINS that bind near the head groups of MYOSIN HEAVY CHAINS. The myosin light chains have a molecular weight of about 20 KDa and there are usually one essential and one regulatory pair of light chains associated with each heavy chain. Many myosin light chains that bind calcium are considered "calmodulin-like" proteins.
Luminescent Proteins
Spectrin
A high molecular weight (220-250 kDa) water-soluble protein which can be extracted from erythrocyte ghosts in low ionic strength buffers. The protein contains no lipids or carbohydrates, is the predominant species of peripheral erythrocyte membrane proteins, and exists as a fibrous coating on the inner, cytoplasmic surface of the membrane.
Paxillin
Paxillin is a signal transducing adaptor protein that localizes to FOCAL ADHESIONS via its four LIM domains. It undergoes PHOSPHORYLATION in response to integrin-mediated CELL ADHESION, and interacts with a variety of proteins including VINCULIN; FOCAL ADHESION KINASE; PROTO-ONCOGENE PROTEIN PP60(C-SRC); and PROTO-ONCOGENE PROTEIN C-CRK.
HeLa Cells
Tubulin
A microtubule subunit protein found in large quantities in mammalian brain. It has also been isolated from SPERM FLAGELLUM; CILIA; and other sources. Structurally, the protein is a dimer with a molecular weight of approximately 120,000 and a sedimentation coefficient of 5.8S. It binds to COLCHICINE; VINCRISTINE; and VINBLASTINE.
Deoxyribonuclease I
An enzyme capable of hydrolyzing highly polymerized DNA by splitting phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide. This catalyzes endonucleolytic cleavage of DNA yielding 5'-phosphodi- and oligonucleotide end-products. The enzyme has a preference for double-stranded DNA.
Protein Multimerization
Intercellular Junctions
Direct contact of a cell with a neighboring cell. Most such junctions are too small to be resolved by light microscopy, but they can be visualized by conventional or freeze-fracture electron microscopy, both of which show that the interacting CELL MEMBRANE and often the underlying CYTOPLASM and the intervening EXTRACELLULAR SPACE are highly specialized in these regions. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p792)
Transfection
Epithelial Cells
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Peptide Fragments
Adaptor Proteins, Signal Transducing
A broad category of carrier proteins that play a role in SIGNAL TRANSDUCTION. They generally contain several modular domains, each of which having its own binding activity, and act by forming complexes with other intracellular-signaling molecules. Signal-transducing adaptor proteins lack enzyme activity, however their activity can be modulated by other signal-transducing enzymes
Fluorescence Recovery After Photobleaching
A method used to study the lateral movement of MEMBRANE PROTEINS and LIPIDS. A small area of a cell membrane is bleached by laser light and the amount of time necessary for unbleached fluorescent marker-tagged proteins to diffuse back into the bleached site is a measurement of the cell membrane's fluidity. The diffusion coefficient of a protein or lipid in the membrane can be calculated from the data. (From Segen, Current Med Talk, 1995).
Base Sequence
Fluorescent Dyes
Troponin
Sequence Homology, Amino Acid
Time-Lapse Imaging
Desmin
Cattle
Cadherins
Calcium-dependent cell adhesion proteins. They are important in the formation of ADHERENS JUNCTIONS between cells. Cadherins are classified by their distinct immunological and tissue specificities, either by letters (E- for epithelial, N- for neural, and P- for placental cadherins) or by numbers (cadherin-12 or N-cadherin 2 for brain-cadherin). Cadherins promote cell adhesion via a homophilic mechanism as in the construction of tissues and of the whole animal body.
Acanthamoeba
ADP Ribose Transferases
Enzymes that transfer the ADP-RIBOSE group of NAD or NADP to proteins or other small molecules. Transfer of ADP-ribose to water (i.e., hydrolysis) is catalyzed by the NADASES. The mono(ADP-ribose)transferases transfer a single ADP-ribose. POLY(ADP-RIBOSE) POLYMERASES transfer multiple units of ADP-ribose to protein targets, building POLY ADENOSINE DIPHOSPHATE RIBOSE in linear or branched chains.
Enzyme Activation
Proteins
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Immunohistochemistry
Blotting, Western
NIH 3T3 Cells
A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. The cells are useful for DNA transfection and transformation studies. (From ATCC [Internet]. Virginia: American Type Culture Collection; c2002 [cited 2002 Sept 26]. Available from http://www.atcc.org/)
Adherens Junctions
Anchoring points where the CYTOSKELETON of neighboring cells are connected to each other. They are composed of specialized areas of the plasma membrane where bundles of the ACTIN CYTOSKELETON attach to the membrane through the transmembrane linkers, CADHERINS, which in turn attach through their extracellular domains to cadherins in the neighboring cell membranes. In sheets of cells, they form into adhesion belts (zonula adherens) that go all the way around a cell.
Marine Toxins
Heterocyclic Compounds with 4 or More Rings
3T3 Cells
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
Wiskott-Aldrich Syndrome
A rare, X-linked immunodeficiency syndrome characterized by ECZEMA; LYMPHOPENIA; and, recurrent pyogenic infection. It is seen exclusively in young boys. Typically, IMMUNOGLOBULIN M levels are low and IMMUNOGLOBULIN A and IMMUNOGLOBULIN E levels are elevated. Lymphoreticular malignancies are common.
Vimentin
Gels
Cell Adhesion Molecules
Growth Cones
COS Cells
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
Nonmuscle Myosin Type IIA
Intracellular Signaling Peptides and Proteins
Proteins and peptides that are involved in SIGNAL TRANSDUCTION within the cell. Included here are peptides and proteins that regulate the activity of TRANSCRIPTION FACTORS and cellular processes in response to signals from CELL SURFACE RECEPTORS. Intracellular signaling peptide and proteins may be part of an enzymatic signaling cascade or act through binding to and modifying the action of other signaling factors.
Phenotype
Cell Membrane Structures
Stress, Mechanical
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Muscle, Striated
Drosophila Proteins
Cell Nucleus
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
rhoB GTP-Binding Protein
Guanine Nucleotide Exchange Factors
Protein-Serine-Threonine Kinases
Muscle Contraction
Cloning, Molecular
Phosphatidylinositol Phosphates
Microscopy, Electron, Scanning
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Cell Differentiation
Two-Hybrid System Techniques
Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
Magnesium
alpha Catenin
Potassium Chloride
Gene Expression Regulation
GTP-Binding Proteins
Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.
Exocytosis
Morphogenesis
Myofibroblasts
Immunoblotting
Microinjections
p21-Activated Kinases
Chick Embryo
RNA, Small Interfering
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
Naphthalenesulfonates
RNA Interference
A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.
Lilium
A plant genus in the family LILIACEAE generally growing in temperate areas. The word lily is also used in the common names of many plants of other genera that resemble true lilies. True lilies are erect perennial plants with leafy stems, scaly bulbs, usually narrow leaves, and solitary or clustered flowers.
Structural features of LIM kinase that control effects on the actin cytoskeleton. (1/626)
LIM kinase phosphorylates and inactivates the actin binding/depolymerizing factor cofilin and induces actin cytoskeletal changes. Several unique structural features within LIM kinase were investigated for their roles in regulation of LIM kinase activity. Disruption of the second LIM domain or the PDZ domain or deletion of the entire amino terminus increased activity in vivo measured as increasing aggregation of the actin cytoskeleton. A kinase-deleted alternate splice product was identified and characterized. This alternate splice product and a kinase inactive mutant inhibited LIM kinase in vivo, indicating that the amino terminus suppresses activity of the kinase domain. Mutation of threonine 508 in the activation loop to valine abolished activity whereas replacement with 2 glutamic acid residues resulted in a fully active enzyme. Dephosphorylation of LIM kinase inhibited cofilin phosphorylation. Mutation of the basic insert in the activation loop inhibited activity in vivo, but not in vitro. These results indicate phosphorylation is an essential regulatory feature of LIM kinase and indicate that threonine 508 and the adjacent basic insert sequences of the activation loop are required for this process. A combination of structural features are thus involved in receiving upstream signals that regulate LIM kinase-induced actin cytoskeletal reorganization. (+info)Ischemia activates actin depolymerizing factor: role in proximal tubule microvillar actin alterations. (2/626)
Apical membrane of renal proximal tubule cells is extremely sensitive to ischemia, with structural alterations occurring within 5 min. These changes are felt secondary to actin cytoskeletal disruption, yet the mechanism responsible is unknown. Actin depolymerizing factor (ADF), a 19-kDa actin-binding protein, has recently been shown to play an important role in regulation of actin filament dynamics. Because ADF is known to mediate pH-dependent F-actin binding, depolymerization, and severing, and because ADF activation occurs by dephosphorylation, we questioned whether ADF played a role in microvilli microfilament disruption during ischemia. To test our hypothesis, we induced renal ischemia in the rat with the clamp model. Initial immunofluorescence and Western blot studies on cortical tissue documented the presence of ADF in proximal tubule cells. Under physiological conditions, ADF was distributed homogeneously throughout the cytoplasm, primarily in the Triton X-100-soluble fraction, and both phosphorylated (pADF) and nonphosphorylated forms were identified. During ischemia, marked alterations occurred. Intraluminal vesicle/bleb structures contained extremely high concentrations of ADF along with G-actin, but not F-actin. Western blot showed a rapidly occurring duration-dependent dephosphorylation of ADF. At 0-30 min of ischemia, total ADF levels were unchanged, whereas pADF decreased significantly to 72% and 19% of control levels, at 5 and 15 min, respectively. Urine collected under physiological conditions did not contain ADF or actin, whereas urine collected after 30 min of ischemia contained both ADF and actin. Reperfusion was associated with normalization of cellular pADF levels, pADF intracellular distribution, and repair of apical microvilli. These data suggest that activation of ADF during ischemia via dephosphorylation is, in part, responsible for apical actin disruption resulting in microvillar destruction and formation of intraluminal vesicles. (+info)XAIP1: a Xenopus homologue of yeast actin interacting protein 1 (AIP1), which induces disassembly of actin filaments cooperatively with ADF/cofilin family proteins. (3/626)
We carried out affinity column chromatography using Xenopus ADF/cofilin (XAC), identified several polypeptides in oocytes specifically bound to this column with actin, and isolated a full-length cDNA clone for a 65 kDa protein in this fraction. The predicted amino acid sequence revealed that the 65 kDa protein has seven obvious WD repeats and exhibits striking homology with yeast actin interacting protein 1 (AIP1). Thus, we designated this protein Xenopus AIP1 (XAIP1). We purified XAIP1 from Xenopus oocytes, and its interaction with actin was characterized by a pelleting assay, photometrical analysis and electron microscopy. Although XAIP1 itself cosedimented with F-actin and increased unsedimented actin to some extent, it induced a rapid, drastic disassembly of actin filaments associated with XAC. Electron microscopic observation revealed that XAIP1 severs actin filaments in the presence of XAC. To elucidate the in vivo effects of XAIP1, the purified protein was injected into blastomeres at the two-cell stage. Although the localization of XAIP1 was similar to that of XAC, at the cortical cytoskeleton and diffusely in the cytoplasm, injection of a large amount of XAIP1 arrested development and abolished the strong cortical staining of both actin and XAC. From these results, we concluded that XAIP1 regulates the dynamics of the cortical actin cytoskeleton cooperatively with XAC in eggs. (+info)Participation of cofilin in opsonized zymosan-triggered activation of neutrophil-like HL-60 cells through rapid dephosphorylation and translocation to plasma membranes. (4/626)
We studied the roles of cofilin, an actin-binding phosphoprotein, in superoxide production of neutrophil-like HL-60 cells triggered by opsonized zymosan (OZ). OZ caused dephosphorylation of cofilin as well as a transient increase of F-actin. Both reactions were complete within 30 s. Okadaic acid (OA) magnified the OZ-triggered O2--production 3.3-fold at 1 microM, but inhibited it completely at 5 microM. We used these critical concentrations to study the effects of OA on changes in phosphorylation and intracellular localization of cofilin. The OZ-induced dephosphorylation of cofilin was inhibited by 5 microM OA but not by 1 microM OA. Subcellular fractionation and immunoblotting revealed that 1 microM OA increased cofilin on the phagosomal membranous fraction but 5 microM OA decreased it. At 1 microM, OA increased translocation of p47phox to membranes, which may explain in part the enhancing effect of 1 microM OA. Confocal laser scanning microscopy showed that: (i) Cofilin diffused throughout the cytosol of resting cells, but accumulated at the plasma membranes forming phagocytic vesicles in activated cells. (ii) At 1 microM, OA had little effect on the OZ-evoked translocation of cofilin, whereas 5 microM OA suppressed it completely. (iii) OA alone, which could not trigger the phagocytic respiratory burst, did not cause any change in the distribution of cofilin at such concentrations. Furthermore, in a superoxide-producing cell-free system employing membranous and cytosolic fractions, affinity-purified anti-cofilin antibody showed an enhancing effect. These results suggest that cofilin participates in the superoxide production of the OZ-activated phagocytes through dephosphorylation and translocation. The roles of cofilin in the activated leukocytes will be discussed. (+info)UNC-60B, an ADF/cofilin family protein, is required for proper assembly of actin into myofibrils in Caenorhabditis elegans body wall muscle. (5/626)
The Caenorhabditis elegans unc-60 gene encodes two functionally distinct isoforms of ADF/cofilin that are implicated in myofibril assembly. Here, we show that one of the gene products, UNC-60B, is specifically required for proper assembly of actin into myofibrils. We found that all homozygous viable unc-60 mutations resided in the unc-60B coding region, indicating that UNC-60B is responsible for the Unc-60 phenotype. Wild-type UNC-60B had F-actin binding, partial actin depolymerizing, and weak F-actin severing activities in vitro. However, mutations in UNC-60B caused various alterations in these activities. Three missense mutations resulted in weaker F-actin binding and actin depolymerizing activities and complete loss of severing activity. The r398 mutation truncated three residues from the COOH terminus and resulted in the loss of severing activity and greater actin depolymerizing activity. The s1307 mutation in a putative actin-binding helix caused greater activity in actin-depolymerizing and severing. Using a specific antibody for UNC-60B, we found varying protein levels of UNC-60B in mutant animals, and that UNC-60B was expressed in embryonic muscles. Regardless of these various molecular phenotypes, actin was not properly assembled into embryonic myofibrils in all unc-60 mutants to similar extents. We conclude that precise control of actin filament dynamics by UNC-60B is required for proper integration of actin into myofibrils. (+info)Mechanism of interaction of Acanthamoeba actophorin (ADF/Cofilin) with actin filaments. (6/626)
We characterized the interaction of Acanthamoeba actophorin, a member of ADF/cofilin family, with filaments of amoeba and rabbit skeletal muscle actin. The affinity is about 10 times higher for muscle actin filaments (Kd = 0.5 microM) than amoeba actin filaments (Kd = 5 microM) even though the affinity for muscle and amoeba Mg-ADP-actin monomers (Kd = 0.1 microM) is the same (Blanchoin, L., and Pollard, T. D. (1998) J. Biol. Chem. 273, 25106-25111). Actophorin binds slowly (k+ = 0.03 microM-1 s-1) to and dissociates from amoeba actin filaments in a simple bimolecular reaction, but binding to muscle actin filaments is cooperative. Actophorin severs filaments in a concentration-dependent fashion. Phosphate or BeF3 bound to ADP-actin filaments inhibit actophorin binding. Actophorin increases the rate of phosphate release from actin filaments more than 10-fold. The time course of the interaction of actophorin with filaments measured by quenching of the fluorescence of pyrenyl-actin or fluorescence anisotropy of rhodamine-actophorin is complicated, because severing, depolymerization, and repolymerization follows binding. The 50-fold higher affinity of actophorin for Mg-ADP-actin monomers (Kd = 0.1 microM) than ADP-actin filaments provides the thermodynamic basis for driving disassembly of filaments that have hydrolyzed ATP and dissociated gamma-phosphate. (+info)Arp2/3 complex and actin depolymerizing factor/cofilin in dendritic organization and treadmilling of actin filament array in lamellipodia. (7/626)
The leading edge (approximately 1 microgram) of lamellipodia in Xenopus laevis keratocytes and fibroblasts was shown to have an extensively branched organization of actin filaments, which we term the dendritic brush. Pointed ends of individual filaments were located at Y-junctions, where the Arp2/3 complex was also localized, suggesting a role of the Arp2/3 complex in branch formation. Differential depolymerization experiments suggested that the Arp2/3 complex also provided protection of pointed ends from depolymerization. Actin depolymerizing factor (ADF)/cofilin was excluded from the distal 0.4 micrometer++ of the lamellipodial network of keratocytes and in fibroblasts it was located within the depolymerization-resistant zone. These results suggest that ADF/cofilin, per se, is not sufficient for actin brush depolymerization and a regulatory step is required. Our evidence supports a dendritic nucleation model (Mullins, R.D., J.A. Heuser, and T.D. Pollard. 1998. Proc. Natl. Acad. Sci. USA. 95:6181-6186) for lamellipodial protrusion, which involves treadmilling of a branched actin array instead of treadmilling of individual filaments. In this model, Arp2/3 complex and ADF/cofilin have antagonistic activities. Arp2/3 complex is responsible for integration of nascent actin filaments into the actin network at the cell front and stabilizing pointed ends from depolymerization, while ADF/cofilin promotes filament disassembly at the rear of the brush, presumably by pointed end depolymerization after dissociation of the Arp2/3 complex. (+info)Aip1p interacts with cofilin to disassemble actin filaments. (8/626)
Actin interacting protein 1 (Aip1) is a conserved component of the actin cytoskeleton first identified in a two-hybrid screen against yeast actin. Here, we report that Aip1p also interacts with the ubiquitous actin depolymerizing factor cofilin. A two-hybrid-based approach using cofilin and actin mutants identified residues necessary for the interaction of actin, cofilin, and Aip1p in an apparent ternary complex. Deletion of the AIP1 gene is lethal in combination with cofilin mutants or act1-159, an actin mutation that slows the rate of actin filament disassembly in vivo. Aip1p localizes to cortical actin patches in yeast cells, and this localization is disrupted by specific actin and cofilin mutations. Further, Aip1p is required to restrict cofilin localization to cortical patches. Finally, biochemical analyses show that Aip1p causes net depolymerization of actin filaments only in the presence of cofilin and that cofilin enhances binding of Aip1p to actin filaments. We conclude that Aip1p is a cofilin-associated protein that enhances the filament disassembly activity of cofilin and restricts cofilin localization to cortical actin patches. (+info)
The Caenorhabditis elegans unc-78 Gene Encodes a Homologue of Actin-Interacting Protein 1 Required for Organized Assembly of...
Publications - Actin dynamics
Beads, bacteria and actin. Cofilin promotes rapid actin filament turnover in vivo. Reconstitution of actin-based motility of...
Interaction of pollen-specific actin-depolymerizing factor with actin
Actin filament disassembling activity of Caenorhabditis elegans actin-interacting protein 1 (UNC-78) is dependent on filament...
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SSH1 - Wikipedia
Dynamic cofilin phosphorylation in the control of lamellipodial actin homeostasis | Journal of Cell Science
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Cofilin 1 - Wikipedia
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Nigerian forex
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Pyr1
The latter is the enzyme that uses ATP to phosphorylate and inactivate the actin-depolymerizing factor cofilin. When cofilin is ... LIMK1 also depolymerizes microtubules. In the presence of Pyr1, LIMK1 is inhibited, which means that the phosphorylation of ... Pyr1 may be used in cancer treatment, because its main target enzyme (LIM kinase) is a regulator of microtubule and actin ... In conclusion, Pyr1 inhibits cell motility and controls actin dynamics and stabilizes microtubules. These properties can be ...
Destrin
The product of this gene belongs to the actin-binding proteins ADF (Actin-Depolymerizing Factor)/cofilin family. This family of ... This gene encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin ... "Human actin depolymerizing factor mediates a pH-sensitive destruction of actin filaments". Biochemistry. 32 (38): 9985-93. doi: ... Destrin or DSTN (also known as actin depolymerizing factor or ADF) is a protein which in humans is encoded by the DSTN gene. ...
TWF1
2003). "Structural conservation between the actin monomer-binding sites of twinfilin and actin-depolymerizing factor (ADF)/ ... Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to ... Palmgren S, Vartiainen M, Lappalainen P (2002). "Twinfilin, a molecular mailman for actin monomers". J. Cell Sci. 115 (Pt 5): ... 2003). "The two ADF-H domains of twinfilin play functionally distinct roles in interactions with actin monomers". Mol. Biol. ...
Ashish Arora
"NMR assignments of actin depolymerizing factor (ADF) like UNC-60A and cofilin like UNC-60B proteins of Caenorhabditis elegans ...
SSH2
The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin ... Niwa R, Nagata-Ohashi K, Takeichi M, Mizuno K, Uemura T (Feb 2002). "Control of actin reorganization by Slingshot, a family of ... The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002).[ ...
SSH3
The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin ... Niwa R, Nagata-Ohashi K, Takeichi M, Mizuno K, Uemura T (January 2002). "Control of actin reorganization by Slingshot, a family ... The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002).[ ...
SSH1
The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin ... Niwa R, Nagata-Ohashi K, Takeichi M, Mizuno K, Uemura T (Feb 2002). "Control of actin reorganization by Slingshot, a family of ... 2006). "Identification of multiple actin-binding sites in cofilin-phosphatase Slingshot-1L". FEBS Lett. 580 (7): 1789-94. doi: ... The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002).[ ...
Axon guidance
While the repulsive cue, Slit, is suggested to stimulate the translation of Cofilin (an actin depolymerizing factor) in growth ... 2006). "Asymmetrical β-actin mRNA translation in growth cones mediates attractive turning to netrin-1". Nature Neuroscience. 9 ... The attractive cue Netrin-1, stimulates mRNA transport and influence synthesis of β-Actin in filopodia of growth cones, to ... and L1 Growth factors like NGF Neurotransmitters and modulators like GABA Growing axons rely on a variety of guidance cues in ...
Cofilin-2
"The three mouse actin-depolymerizing factor/cofilins evolved to fulfill cell-type-specific requirements for actin dynamics". ... Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and ... Bamburg JR, McGough A, Ono S (September 1999). "Putting a new twist on actin: ADF/cofilins modulate actin dynamics". Trends ... 2006). "Cofilin cross-bridges adjacent actin protomers and replaces part of the longitudinal F-actin interface". J. Mol. Biol. ...
Suzanne Zukin
Elevated expression of proteins in this pathway are causally related to reduced activity of the actin depolymerizing factor ... They investigate the role of the gene silencing transcription factor RE1-silencing transcription factor (REST) and REST- ... "REST-dependent epigenetic remodeling of NMDA receptors as a risk factor in schizophrenia." Frontiers in Neuroscience Genetics ... "Repressor element-1 silencing transcription factor (REST)-dependent epigenetic remodeling is critical to ischemia-induced ...
Plasma gelsolin
Around the same time a similarly sized plasma protein was discovered and shown to depolymerize actin; it was named Brevin, due ... Chaponnier, C.; Borgia, R.; Rungger-Brändle, E.; Weil, R.; Gabbiani, G. (1979-08-15). "An actin-destabilizing factor is present ... Furthermore, DBP is capable of removing one actin from a 2:1 actin-pGSN complex, restoring its ability to sever F-actin. F- ... "Interactions of gelsolin and gelsolin-actin complexes with actin. Effects of calcium on actin nucleation, filament severing, ...
ROCK1
... inhibiting its actin-depolymerizing activity. This depolymerization results in stabilization of actin filaments and decreased ... Gilkes DM, Xiang L, Lee SJ, Chaturvedi P, Hubbi ME, Wirtz D, Semenza GL (January 2014). "Hypoxia-inducible factors mediate ... It is a key regulator of actin-myosin contraction, stability, and cell polarity. These contribute to many progresses such as ... This is consistent with its function as a key modulator of cell motility, tumor cell invasion, and actin cytoskeleton ...
List of MeSH codes (D05)
Actin depolymerizing factors MeSH D05.750.078.730.212.500 - cofilin 1 MeSH D05.750.078.730.212.750 - cofilin 2 MeSH D05.750. ... actin-related protein 2 MeSH D05.750.078.730.246.750 - actin-related protein 3 MeSH D05.750.078.730.250 - actins MeSH D05.750. ... actin capping proteins MeSH D05.750.078.730.032.500 - capz actin capping protein MeSH D05.750.078.730.032.750 - tropomodulin ... 078.730.212.875 - destrin MeSH D05.750.078.730.246 - actin-related protein 2-3 complex MeSH D05.750.078.730.246.500 - ...
ADF-H domain
"Structural conservation between the actin monomer-binding sites of twinfilin and actin-depolymerizing factor (ADF)/cofilin". J ... ADF/cofilins bind ADP-actin with higher affinity than ATP-actin and inhibit the spontaneous nucleotide exchange on actin ... In molecular biology, ADF-H domain (actin-depolymerising factor homology domain) is an approximately 150 amino acid motif that ... They bind both actin-monomers and filaments and promote rapid filament turnover in cells by depolymerising/fragmenting actin ...
Actin, cytoplasmic 2
"Human actin depolymerizing factor mediates a pH-sensitive destruction of actin filaments". Biochemistry. 32 (38): 9985-93. doi: ... Actin, cytoplasmic 2, or gamma-actin is a protein that in humans is encoded by the ACTG1 gene. Gamma-actin is widely expressed ... Gamma-actin is eventually replaced by sarcomeric alpha-actin isoforms, with low levels of gamma-actin persisting in adult ... Human gamma-actin is 41.8 kDa in molecular weight and 375 amino acids in length. Actins are highly conserved proteins that are ...
Actin remodeling of neurons
Actin Depolymerizing Factor, or ADF, normally disassembles actin and hampers the induction of LTP. However, synaptic activity ... Actin treadmilling is the process of turnover of actin filaments where F-actin is rapidly assembled and disassembled. G-actin ... This protein caps the barbed end of F-actin, thus preventing G-actin subunits from binding to F-actin and blocking actin ... Actin is only able to cause changes that promote LTP through its formation into F-actin. When F-actin is unable to form, LTD is ...
Villin 1
"Functional dissection and molecular characterization of calcium-sensitive actin-capping and actin-depolymerizing sites in ... "Helicobacter-induced intestinal metaplasia in the stomach correlates with Elk-1 and serum response factor induction of villin ... This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of ... Revenu C, Courtois M, Michelot A, Sykes C, Louvard D, Robine S (March 2007). "Villin severing activity enhances actin-based ...
Trastuzumab
... and of the actin-depolymerizing protein cofilin, mediated by EGFR/ERBB1. Furthermore, this latter event may be inhibited by the ... The HER (human epidermal growth factor receptor) protein, binds to human epidermal growth factor, and stimulates cell ... trastuzumab suppresses angiogenesis both by induction of antiangiogenic factors and repression of proangiogenic factors. It is ... CDR formation requires activation of both the protein regulator of actin polymerization N-WASP, mediated by ERK1/2, ...
Bleb (cell biology)
Breakage of cortex-membrane bonds has also been caused by laser ablation and injection of an actin depolymerizing drug, which ... The presence of only one or two of these factors is often not enough to drive bleb formation. Bleb formation has also been ... This interaction stabilizes a form of myosin II that is not bound to actin, thus lowering the affinity of myosin with actin. By ... and deterioration of the actin cortex. The integrity of the connection between the actin cortex and the membrane are dependent ...
NADPH oxidase
... depolymerized the actin, broke the adhesions, and allowed foam cells to migrate out of the intima. One study suggests a role ... These include thrombin, platelet-derived growth factor (PDGF), tumor necrosis factor (TNFa), lactosylceramide, interleukin-1, ... Vascular NADPH oxidases are regulated by a variety of hormones and factors known to be important players in vascular remodeling ... by polymerizing actin fibers). This process is counterbalanced by NADPH oxidase inhibitors, and by antioxidants. An imbalance ...
Tubulin alpha-1A chain
Watts NR, Sackett DL, Ward RD, Miller MW, Wingfield PT, Stahl SS, Steven AC (July 2000). "HIV-1 rev depolymerizes microtubules ... Sapir T, Elbaum M, Reiner O (December 1997). "Reduction of microtubule catastrophe events by LIS1, platelet-activating factor ... interaction with actin, clathrin and tubulin". The Biochemical Journal. 363 (Pt 3): 599-608. doi:10.1042/0264-6021:3630599. PMC ... Sapir T, Elbaum M, Reiner O (December 1997). "Reduction of microtubule catastrophe events by LIS1, platelet-activating factor ...
Tropomyosin
"Tropomyosin binding to F-actin protects the F-actin from disassembly by brain actin-depolymerizing factor (ADF)". Cell Motil. 2 ... it was observed that the actin-binding protein actin depolymerisation factor (ADF)/cofilin, a factor that promotes actin ... The actin filament system that is involved in regulating these cellular pathways is more complex than the actin filament ... The tropomyosin dimer has very low affinity for an actin filament and forms no van der waals contacts with actin. It is only ...
Microtubule
Signals sent between the follicular cells and the oocyte (such as factors similar to epidermal growth factor) cause the ... Recently an actin-like protein has been found in the gram-positive bacterium Bacillus thuringiensis, which forms a microtubule- ... However, once the microtubule depolymerizes, most of these modifications are rapidly reversed by soluble enzymes. Since most ... After nucleation, the minus-ends are released and then re-anchored in the periphery by factors such as ninein and PLEKHA7. In ...
CDC42
Miki H, Sasaki T, Takai Y, Takenawa T (January 1998). "Induction of filopodium formation by a WASP-related actin-depolymerizing ... This process is regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, ... "GRB2 links signaling to actin assembly by enhancing interaction of neural Wiskott-Aldrich syndrome protein (N-WASp) with actin- ... Rho GTPases are central to dynamic actin cytoskeletal assembly and rearrangement that are the basis of cell-cell adhesion and ...
Cytoskeleton
Actin cables are bundles of actin filaments and are involved in the transport of vesicles towards the cap (which contains a ... Prior to the work of Jones et al., 2001, the cell wall was believed to be the deciding factor for many bacterial cell shapes, ... The network influences cell mechanics and dynamics by differentially polymerizing and depolymerizing its constituent filaments ... Actin-like proteins are actin in eukaryotes and MreB, FtsA in prokaryotes. An example of a WACA-proteins, which are mostly ...
Golgi apparatus
Organization of the plant Golgi depends on actin cables and not microtubules. The common feature among Golgi is that they are ... BFA inhibits the function of several guanine nucleotide exchange factors (GEFs) that mediate GTP-binding of ARFs. Treatment of ... In experiments it is seen that as microtubules are depolymerized the Golgi apparatuses lose mutual connections and become ... BFA blocks the activation of some ADP-ribosylation factors (ARFs). ARFs are small GTPases which regulate vesicular trafficking ...
LIMK2
... inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the ... "LIM-kinase 2 and cofilin phosphorylation mediate actin cytoskeleton reorganization induced by transforming growth factor-beta ... Sumi T, Matsumoto K, Takai Y, Nakamura T (27 Dec 1999). "Cofilin phosphorylation and actin cytoskeletal dynamics regulated by ... Toshima J, Toshima JY, Takeuchi K, Mori R, Mizuno K (Aug 2001). "Cofilin phosphorylation and actin reorganization activities of ...
Myosin light-chain kinase
ROCK indirectly strengthens actin/myosin contraction through inhibiting Cofilin, a protein which depolymerizes actin stress ... Wang F, Graham WV, Wang Y, Witkowski ED, Schwarz BT, Turner JR (February 2005). "Interferon-gamma and tumor necrosis factor- ... On the other side of the kinase at the N-Terminus end, sits the actin-binding domain, which allows MYLK to form interactions ... However, when the inward pulling force of the actin stress fiber becomes greater than the outward pulling force of the cell ...
Cell migration
If so, the actin filaments that form there might stabilize the added membrane so that a structured extension, or lamella, is ... On the other hand, high drug concentrations, or microtubule mutations that depolymerize the microtubules, can restore cell ... "2D protrusion but not motility predicts growth factor-induced cancer cell migration in 3D collagen". J. Cell Biol. 197 (6): 721 ... Drugs that destroy actin filaments have multiple and complex effects, reflecting the wide role that these filaments play in ...
Congenital Myopathies Clinical Presentation: History, Nemaline Rod Myopathy, Core Myopathy
23] Cofilins are actin-modulating proteins that act to depolymerize F-actin and inhibit the polymerization of G-actin. Cofilin- ... One remarkable factor is that weakness is distal more than proximal, resulting in delayed major motor milestones, but ... 13] The actin monomer, G-actin (has binding site for myosin) polymerizes to form F-actin. Two strands of F-actin combine in a ... Myotilin binds actin and is thought to be involved in stabilization of actin bundles and anchorage of thin filaments at the Z ...
Isolation and characterization of a regulated form of actin depolymerizing factor | Journal of Cell Biology | Rockefeller...
... is an 18.5-kD protein with pH-dependent reciprocal F-actin binding and severing/depolymerizing activities. We previously showe ... Actin depolymerizing factor (ADF) is an 18.5-kD protein with pH-dependent reciprocal F-actin binding and severing/ ... Actin Depolymerizing Factor (ADF/Cofilin) Enhances the Rate of Filament Turnover: Implication in Actin-based Motility ... Isolation and characterization of a regulated form of actin depolymerizing factor TE Morgan, TE Morgan ...
GSN gene: MedlinePlus Genetics
actin-depolymerizing factor. *ADF. *AGEL. *brevin. *DKFZp313L0718. *GELS_HUMAN. *gelsolin isoform a precursor ... Interactions of gelsolin and gelsolin-actin complexes with actin. Effects of calcium on actin nucleation, filament severing, ... to another protein called actin. Actin proteins are organized into filaments, which form a network (the cytoskeleton) that ... Gelsolin helps assemble or disassemble actin filaments. It is thought that, through this function, the gelsolin protein ...
The two Caenorhabditis elegans actin-depolymerizing factor/cofilin proteins differently enhance actin filament severing and...
N2 - Actin-depolymerizing factor (ADF)/cofilin enhances the turnover of actin filaments by two separable activities: filament ... AB - Actin-depolymerizing factor (ADF)/cofilin enhances the turnover of actin filaments by two separable activities: filament ... Actin-depolymerizing factor (ADF)/cofilin enhances the turnover of actin filaments by two separable activities: filament ... abstract = "Actin-depolymerizing factor (ADF)/cofilin enhances the turnover of actin filaments by two separable activities: ...
Cucurbitacin I inhibits cell motility by indirectly interfering with actin dynamics
Cucurbitacin I results in accumulation of actin filaments in cells by a unique indirect mechanism. Furthermore, the proximal ... Actin Cytoskeleton / metabolism * Actin Cytoskeleton / ultrastructure * Actin Depolymerizing Factors / metabolism * Actins / ... Therefore, cucurbitacin I targets some factor involved in cellular actin dynamics other than actin itself. Two candidate ... In in vitro actin depolymerization experiments, cucurbitacin I had no effect on the rate of actin filament disassembly at the ...
Actin, cytoplasmic 2 - Wikipedia
"Human actin depolymerizing factor mediates a pH-sensitive destruction of actin filaments". Biochemistry. 32 (38): 9985-93. doi: ... Actin, cytoplasmic 2, or gamma-actin is a protein that in humans is encoded by the ACTG1 gene. Gamma-actin is widely expressed ... Gamma-actin is eventually replaced by sarcomeric alpha-actin isoforms, with low levels of gamma-actin persisting in adult ... Human gamma-actin is 41.8 kDa in molecular weight and 375 amino acids in length. Actins are highly conserved proteins that are ...
Abl Tyrosine Kinase Promotes Dendrogenesis by Inducing Actin Cytoskeletal Rearrangements in Cooperation with Rho Family Small...
Meberg PJ, Bamburg JR (2000) Increase in neurite outgrowth mediated by overexpression of actin depolymerizing factor. J ... Abl kinases are known to organize actin rearrangement both directly, via binding domains for G-actin and F-actin, and ... F-actin staining is weak because of competitive binding of jasplakinolide and phalloidin to F-actin. Note that F-actin staining ... A the next day for 24 hr to depolymerize actin filament or with 100 nm jasplakinolide on 7 DIV for 5 hr to stabilize actin ...
Frontiers | Dendritic Actin Cytoskeleton: Structure, Functions, and Regulations
... the regulation of actin dynamics by internal and external factors, and the role of F-actin in dendritic protein trafficking. ... the regulation of actin dynamics by internal and external factors, and the role of F-actin in dendritic protein trafficking. ... Apart from that striking feature, patches of F-actin and deep actin filament bundles have been described along the lengths of ... Apart from that striking feature, patches of F-actin and deep actin filament bundles have been described along the lengths of ...
SMART: CH domain annotation
F-actin and G-actin binding are uncoupled by mutation of conserved tyrosine residues in maize actin depolymerizing factor ( ... They bind both monomeric actin (G-actin) and filamentous actin (F-actin) and, under certain conditions, F-actin binding is ... Actin depolymerizing factors (ADF) are stimulus responsive actin cytoskeleton modulating proteins. ... Binding was specific for F-actin because G-actin did not bind. Further, unlabeled F-actin blocked the binding of 125I-labeled F ...
William Wood - Research output - University of Edinburgh Research Explorer
Actin Depolymerizing Factors 41% * Myosins 32% * Drosophila 27% * Phagocyte Responses to Cell Death in Flies.. Davidson, A. J. ... Dynamic analysis of actin cable function during Drosophila dorsal closure. Jacinto, A., Wood, W., Woolner, S., Hiley, C., ... Dynamic actin-based epithelial adhesion and cell matching during Drosophila dorsal closure. Jacinto, A., Wood, W., Balayo, T., ... Macrophages Use Distinct Actin Regulators to Switch Engulfment Strategies and Ensure Phagocytic Plasticity In Vivo. Davidson, A ...
DataCite Search
The actin-depolymerizing factor/cofilin (ADF/CFL) gene family encodes a diverse group of relatively small proteins. Once known ... Data from: Phylogenetic patterns of codon evolution in the actin-depolymerizing factor/cofilin (adf/cfl) gene family Eileen M. ... Here, we investigate how factors at the individual level might constrain the composition of gut microbes in an obligate ... from signal transduction to the cytonuclear trafficking of actin. In both plant and animal lineages, expression patterns of ...
Pharmacological Stimulation of the Brain Serotonin Receptor 7 as a Novel Therapeutic Approach for Rett Syndrome |...
In the brain of RTT mice, LP-211 also reversed the abnormal activation of PAK and cofilin (key regulators of actin cytoskeleton ... an actin filament depolymerizing/severing factor (De Filippis et al, in press). In this line, alterations in these signaling ... Right: semiquantitative densitometric analysis, obtained by OD of protein bands normalized with OD of β-actin bands. OD ratios ... In the brain of RTT mice, LP-211 also reversed the abnormal activation of PAK and cofilin (key regulators of actin cytoskeleton ...
Plus it
2006) Phosphorylation of actin-depolymerizing factor/cofilin by LIM-kinase mediates amyloid β-induced degeneration: a potential ... A, Immunoblotting was used to measure phosphorylation of the actin depolymerizing protein cofilin, and its upstream regulator ... Both pathways ultimately converge on the actin-binding protein cofilin, which promotes collapse through actin depolymerization ... 2010) Learning, AMPA receptor mobility and synaptic plasticity depend on n-cofilin-mediated actin dynamics. EMBO J 29:1889-1902 ...
Background
Transcription factor AP 4. EgrG_000768400.1. 0. 0,00621390. -. -. Actin-depolymerizing factor 2. gi,576697711. 0,00135023. 0, ... Another factor contributing to the success of AHA-labeling is that the genome of this parasite showed no machinery for the ... Factors influencing the development and carbohydrate metabolism of Echinococcus granulosus in dogs. . J Parasitol. . 1998. ;. ... The normalized spectral abundance factor (NSAF) [. 28. ] was calculated for each protein, and the quantitative differences were ...
PARK7 Diminishes Oxidative Stress-Induced Mucosal Damage in Celiac Disease
D. Wang, N. G. Naydenov, A. Feygin, S. Baranwal, J. F. Kuemmerle, and A. I. Ivanov, "Actin-depolymerizing factor and cofilin-1 ... ZO-1 co-localized with the actin filaments. In H2O2 treated cells actin fibers aggregated into dots and ZO-1 was released from ... we found that Comp23 treatment increased the expression of nuclear factor erythroid 2-related factor 2 (NRF2) transcription ... C.-L. Wei, Q. Wu, V. B. Vega et al., "A global map of p53 transcription-factor binding sites in the human genome," Cell, vol. ...
BDNF stabilizes synapses and maintains the structural complexity of optic axons in vivo | Development | The Company of...
... neurotrophic factor regulation of retinal growth cone filopodial dynamics is mediated through actin depolymerizing factor/ ... F-actin is enriched at synapses and the integrity of the actin cytoskeleton at pre- or postsynaptic terminals can also directly ... it is likely that BDNF signaling promotes changes in actin polymerization and the reorganization of the actin cytoskeleton at ... Actin polymerization and microtubule dynamics are necessary for growth cone steering and axon branching (Dent et al.,2004; ...
Comparative analysis of alfalfa (Medicago sativa L.) leaf transcriptomes reveals genotype-specific salt tolerance mechanisms |...
... including a transcription factor. Consistent with the suppression of plant growth resulting from salt stress, the expression of ... Soil salinity is an important factor affecting growth, development, and productivity of almost all land plants, including the ... Comprehensive analysis of differentially expressed rice actin depolymerizing factor gene family and heterologous overexpression ... DEGs encoding transcription factors. Salt stress induced or suppressed many more TFs in XJ than in ZM: 158 and 78 TF genes were ...
A 100%-complete sequence reveals unusually simple genomic features in the hot-spring red alga Cyanidioschyzon merolae | BMC...
Jianglin Fan | Semantic Scholar
... a member of theactin depolymerizing factor family that is critical for actin reorganization.. Expand. ... Epidermal Growth Factor Receptor-PI3K Signaling Controls Cofilin Activity To Facilitate Herpes Simplex Virus 1 Entry into ... It is demonstrated that the entry of HSV-1 into neuronal cells induces biphasic remodeling of the actin cytoskeleton and an ... Increased expression of vascular endothelial growth factor in kidney leads to progressive impairment of glomerular functions.. ...
Role of Protein Kinase D in Actin Remodeling and Cell Motiliy - Fingerprint - Mayo Clinic
CoP: Co-expressed Biological Processes
ADF11 (ACTIN DEPOLYMERIZING FACTOR 11). F:actin binding;P:biological_process unknown;C:intracellular;MPOFB. O.I.. C.G.. H.G.. ... F:transcription factor activity, DNA binding;P:regulation of transcription;C:nucleus;P. O.I.. C.G.. H.G.. Please select. TAIR ( ... Encodes a phytosulfokine-alpha (PSK) precursor, a unique plant peptide growth factor first described in Asparagus.. O.I.. C.G. ...
Steven C. Almo - Publications
- Albert Einstein College of Medicine
Actin Depolymerizing Factors 100% * Actins 59% * ethylenediamine 18% * Fluorescence 16% * Yeasts 16% ... Structure and dynamics of the actin filament. Guan, J. Q., Takamoto, K., Almo, S. C., Reisler, E. & Chance, M. R., Mar 8 2005, ... Structural effects of cofilin on longitudinal contacts in F-actin. Bobkov, A. A., Muhlrad, A., Kokabi, K., Vorobiev, S., Almo, ... Structural and functional dissection of the Abp1 ADFH actin-binding domain reveals versatile in vivo adapter functions. ...
Finding a niche: studies from the Drosophila ovary | Stem Cell Research & Therapy | Full Text
The actin-depolymerizing factor Cofilin/ADF, encoded by the twinstar gene, regulates the actin cytoskeletal rearrangements that ... Fibroblast Growth Factors, Transforming Growth Factor beta and Hedgehog [46]. In Drosophila, Dally promotes the stability and ... Systemic factors that vary in response to diet and age play an important role in modulating niche output and stem cell ... A second transcription factor Engrailed also marks terminal filaments and is necessary for their development [36]. Identifying ...
Romet Lemonne Jegou Lab VA - Institut Jacques Monod
Quantitative Variations with pH of Actin Depolymerizing Factor/Cofilins Multiple Actions on Actin Filaments. Biochemistry 58, ... If ABPs can modify the mechanical state of actin filaments, external mechanical factors can in turn affect the activity of ABPs ... In cells, actin assembly is regulated by hundreds of actin binding proteins (ABPs), which can act together, in synergy, or in ... The assembly of ATP-actin monomers into filaments is the basis for the formation of the actin cytoskeleton in cells. The ...
Effect of phalloidin on liver actin distribution, content, and turnover | Archive ouverte UNIGE
We studied the sensitivity of immunofluorescent staining of actin to an actin depolymerizing factor (ADF) as well as actin ... Actin Depolymerizing Factors - Actins/analysis/biosynthesis/ metabolism - Animals - Destrin - Fluorescent Antibody Technique - ... Thus, phalloidin appears to stabilize actin against the depolymerizing actions of ADF, increases the proportion of F-actin ... Phalloidin increases F-actin microfilament content and actin-directed immunofluorescence in hepatocytes in vivo and also ...
Difference between revisions of "Medicago sativa" - ICG
یا راک | دانلود مقالات ISI یا راک | 466 مقاله انگلیسی + ترجمه فارسی
Keywords: یا راک; ABP; actin-binding protein; ADF; actin-depolymerizing factor; Aip1; actin-interacting protein 1; Arp; actin- ... actin binding protein 1; ACTN4; α-actinin-4; ADF; actin-depolymerizing factor; ADHD; attention deficit/hyperactivity disorder ... Keywords: یا راک; BBB; blood-brain barrier; BDNF; brain-derived neurotrophic factor; FGFR; fibroblast growth factor receptor; ... actin beta/beta-actin; ASCL1; achaete-scute homolog 1 (MASH1); ASOs; anti-sense oligonucleotides; Bcl-xL; b-cell lymphoma-extra ...
Jonathan M. Backer - Publications
- Albert Einstein College of Medicine
Epidermal Growth Factor 40% * Actin-Related Protein 2-3 Complex 31% * Actin Depolymerizing Factors 29% ... Specific requirement for the p85-p110α phosphatidylinositol 3-kinase during epidermal growth factor-stimulated actin nucleation ... Transforming growth factor β activates Smad2 in the absence of receptor endocytosis. Lu, Z., Murray, J. T., Luo, W., Li, H., Wu ... Distinct roles for the p110α and hVPS34 phosphatidylinositol 3- kinases in vesicular trafficking, regulation of the actin ...
Putative flagellar proteins
David R. Kovar's research topics | Profiles RNS
Actin Depolymerizing Factors. Actin-Related Protein 2-3 Complex. Actin Cytoskeleton. Actinin. ... Actins. Tropomyosin. Microscopy, Fluorescence. Caenorhabditis elegans. Schizosaccharomyces. Schizosaccharomyces pombe Proteins ... Actin Depolymerizing Factors. 8. 2003. 2020. May 2013. Actin-Related Protein 2-3 Complex. 8. 2006. 2019. August 2013. ...
Transient assembly of F-actin on the outer mitochondrial membrane contributes to mitochondrial fission<...
Actin-Related Protein 2-3 Complex Medicine & Life Sciences 14% * Actin Depolymerizing Factors Medicine & Life Sciences 13% ... of mitochondrial accumulation of F-actin and also led to abnormal mitochondrial accumulation of the actin regulatory factors ... of mitochondrial accumulation of F-actin and also led to abnormal mitochondrial accumulation of the actin regulatory factors ... of mitochondrial accumulation of F-actin and also led to abnormal mitochondrial accumulation of the actin regulatory factors ...
FilamentsProteinsCofilinMicrotubulesMyosinDynamicsDisassemblyPolymerizationOrganizationMicrofilamentsAppears to stabilizeProtein called actinCapping actin proteinVitroDepolymerizationTurnoverRate of actinRole of actinRoles of actinAssembly of actinComplexCytoplasmicFluorescent ActinCellularGelsolinRhodamine-phalloidinLatticePhosphorylationNeuronalElongationBindSubunitsNucleationCell motilityBundlesMotilityConformationMolecularGeneDestrinProfilinNuclearKinaseFissionTranscription factorsReorganization
Filaments31
- Actin proteins are organized into filaments, which form a network (the cytoskeleton) that gives structure to cells and allows them to change shape and move. (medlineplus.gov)
- Gelsolin helps assemble or disassemble actin filaments. (medlineplus.gov)
- However, we found that, unlike jasplakinolide or phallacidin, cucurbitacin I does not directly stabilize actin filaments. (nih.gov)
- Cucurbitacin I results in accumulation of actin filaments in cells by a unique indirect mechanism. (nih.gov)
- Actin-depolymerizing factor (ADF)/cofilin enhances the turnover of actin filaments by two separable activities: filament severing and pointed-end depolymerization. (elsevier.com)
- Here, we show that the two Caenorhabditis elegans ADF/cofilin isoforms exhibit different activities for severing and depolymerizing actin filaments. (elsevier.com)
- The two primary cytoskeletal components of costameres are desmin intermediate filaments and gamma-actin microfilaments. (wikipedia.org)
- Additional studies have shown that gamma-actin colocalizes with alpha-actinin and GFP-labeled gamma actin localized to Z-discs, whereas GFP-alpha-actin localized to pointed ends of thin filaments, indicating that gamma actin specifically localizes to Z-discs in striated muscle cells. (wikipedia.org)
- The CA-Abl phenotype is not affected by destabilization of microtubules but is reversed partially when actin filaments are stabilized with jasplakinolide. (jneurosci.org)
- For a long time, the most prominent roles that were attributed to actin in neurons were the movement of growth cones, polarized cargo sorting at the axon initial segment, and the dynamic plasticity of dendritic spines, since those compartments contain large accumulations of actin filaments (F-actin) that can be readily visualized using electron- and fluorescence microscopy. (frontiersin.org)
- These domains cross-link actin filaments into bundles and networks. (embl.de)
- A large number of proteins interact with actin to control how, where and when actin monomers assemble into filaments. (ijm.fr)
- The assembly of ATP-actin monomers into filaments is the basis for the formation of the actin cytoskeleton in cells. (ijm.fr)
- If ABPs can modify the mechanical state of actin filaments, external mechanical factors can in turn affect the activity of ABPs. (ijm.fr)
- To understand how ABPs and the mechanical context generate networks of various geometries, dynamics and lifetimes, our team focuses its efforts on the observation and in vitro manipulations of single actin filaments or small reconstituted networks under well-controlled conditions. (ijm.fr)
- Microfluidics is a very powerful tool to expose filaments to different protein solutions in a sequential manner and to expose filaments to various mechanical stresses, thus opening new avenues to decipher the dynamics of actin network assembly. (ijm.fr)
- In this chamber, actin filaments are grown from primers anchored to the surface of the experimental chamber and aligned with the flow. (ijm.fr)
- Dozens of fluorescently labeled actin filaments can be followed in parallel while being exposed to various biochemical conditions affecting their dynamics. (ijm.fr)
- Mechanically tuning actin filaments to modulate the action of actin-binding proteins. (ijm.fr)
- Previous work has demonstrated that tubules can be generated by being pulled out of membrane reservoirs by molecular motors that move along microtubule or actin filaments, or by the tips of filaments as these grow by polymerization. (hmamedicalclinic.com)
- However, the alignment of membrane tubules with the filaments of the cytoskeleton is not perfect, the ER network does not retract upon deplomerization of actin filaments in yeast and retracts only slowly upon depolymerization of microtubules in mammals, and ER tubules can also be generated from vesicles. (hmamedicalclinic.com)
- A family of multidomain microfilament proteins that bind ACTIN FILAMENTS and INTEGRINS at FOCAL ADHESIONS . (nih.gov)
- These proteins can change the mean twist of actin filaments by ∼5° per subunit, but other workers have claimed that this change in twist can be uncoupled from subunit dissociation. (rupress.org)
- also discovered that two molecules of ADF bind per actin subunit, and that some actin subunits are tilted significantly from their normal positions under conditions that destabilize filaments. (rupress.org)
- Harker AJ, Katkar HH, Bidone TC, Aydin F, Voth GA, Applewhite DA, Kovar DR. Ena/VASP processive elongation is modulated by avidity on actin filaments bundled by the filopodia cross-linker fascin. (uchicago.edu)
- Actin filaments are shown in red, and microtubules composed of beta tubulin are in green. (cloudfront.net)
- [9] During contraction of a muscle , within each muscle cell, myosin molecular motors collectively exert forces on parallel actin filaments. (cloudfront.net)
- Unlike other cytoskeletal components in the cell, for example, actin filaments which continuously grow as long as enough G-actin is present, MTs stochastically switch between growing and shrinking phases even under sufficient free tubulin concentrations. (elifesciences.org)
- Also called actin filaments that form the part of the cytoskeleton and are present in the cytoplasm of eukaryotic cells composed of polymers of actin are microfilaments. (difference.wiki)
- Due to the complex nature of actin dynamics, adequate cellular response to extracellular stimuli not only requires polymerization and/or disassembly of actin filaments, but also coordinated synthesis of the myriad of structural proteins and regulatory factors that accompany this process. (biomedcentral.com)
- The driving drive for such membrane transformation is spatially and temporally-regulated by polymerization of submembrane actin filaments. (micchem.com)
Proteins20
- Two candidate proteins that play roles in actin depolymerization are the actin-severing proteins cofilin and gelsolin. (nih.gov)
- Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. (wikipedia.org)
- Actin binding domains present in duplicate at the N-termini of spectrin-like proteins (including dystrophin, alpha-actinin). (embl.de)
- A number of actin-binding proteins, including spectrin, alpha-actinin and fimbrin, contain a 250 amino acid stretch called the actin binding domain (ABD). (embl.de)
- In addition, the CH domain occurs also in a number of proteins not known to bind actin, a notable example being the vav protooncogene. (embl.de)
- Gelation factor (ABP120) is one of the principal actin-cross-linking proteins of Dictyostelium discoideum. (embl.de)
- Our goal is to understand the elementary processes of actin mechanosensitivity affecting actin dynamics and the action of regulatory proteins. (ijm.fr)
- In cells, actin assembly is regulated by hundreds of actin binding proteins (ABPs), which can act together, in synergy, or in competition. (ijm.fr)
- Furthermore, down-regulation of actin regulatory proteins led to elongation of mitochondria, associated with mitochondrial accumulation of Drp1. (elsevier.com)
- In pre-clinical studies, we and others have shown that various protein-protein interactions, pertaining to the actin microfilament-associated proteins, ezrin and cofilin, mediate breast cancer cell migration, a prerequisite for cancer metastasis. (ox.ac.uk)
- Using fluorescence recovery after photobleaching (FRAP) and fluorescence loss in photobleaching (FLIP) analysis of GFP fusion proteins, Chen and Huang observed rapid exchange between the nucleolus and the nucleoplasm of factors involved in rRNA transcription, pre-rRNA processing, and ribosome assembly. (rupress.org)
- Rapid F-actin turnover requires proteins of the highly conserved ADF/cofilin family. (rupress.org)
- They found that segments of pure actin can adopt the ADF/cofilin-like state of twist, even in the absence of other proteins, and, conversely, that the ADF-actin complex can adopt a state of twist close to the normal actin state. (rupress.org)
- In this project, we focus on a family of actin regulatory proteins coordinating the formation of invasive membrane protrusions (also termed invadopodia) and the upregulation of proteases involved in extracellular matrix degradation. (lih.lu)
- The main difference between microtubules and microfilaments is that microtubules are formed of tubulin protein units and normally are long, and hollow cylinders, whereas microfilaments are produced by actin proteins, and usually are double-stranded helical polymers. (difference.wiki)
- conversely, proteins involved in the regulation of the dynamics of microfilaments are filament cross-linkers, actin monomer-binding proteins, actin-related protein 2/3 (Arp2/3) complex, and filament-severing proteins. (difference.wiki)
- [ 7 ] Tropomyosins are a family of actin-binding coiled-coil proteins that help to regulate calcium-dependent muscle contraction. (medscape.com)
- These signals are perceived by various receptor proteins including G protein-coupled receptors (GPCRs), Receptor Tyrosine Kinases (RTKs), and receptors for integrin, transforming growth factor-β (TGFβ), and E-cadherin signaling. (biomedcentral.com)
- Receptors link to Rho GTPases via selective Rho guanine nucleotide exchange factors (GEFs) that activate Rho proteins by catalyzing the exchange of GDP for GTP. (biomedcentral.com)
- Once activated, Rho GTPases regulate numerous downstream effector proteins to modulate actin polymerization chiefly via two well-established pathways, the first involving Rho-associated kinase (ROCK)-LIM kinase-cofilin signaling, and the other mediated by formins. (biomedcentral.com)
Cofilin9
- These results suggest that cofilin-like UNC-60B is kinetically more efficient in enhancing actin turnover than ADF-like UNC-60A, while ADF-like UNC-60A is suitable for maintaining higher concentrations of monomeric actin. (elsevier.com)
- In the brain of RTT mice, LP-211 also reversed the abnormal activation of PAK and cofilin (key regulators of actin cytoskeleton dynamics) and of the ribosomal protein (rp) S6, whose reduced activation in MECP2 mutant neurons by mTOR is responsible for the altered protein translational control. (nature.com)
- It is demonstrated that the entry of HSV-1 into neuronal cells induces biphasic remodeling of the actin cytoskeleton and an initial inactivation followed by the subsequent activation of cofilin, a member of theactin depolymerizing factor family that is critical for actin reorganization. (semanticscholar.org)
- Actin filament oxidation by MICAL1 suppresses protections from cofilin-induced disassembly. (ijm.fr)
- Impairing mitochondrial division through Drp1 knockout or inhibition prolonged the time of mitochondrial accumulation of F-actin and also led to abnormal mitochondrial accumulation of the actin regulatory factors cortactin, cofilin, and Arp2/3 complexes, suggesting that disassembly of mitochondrial F-actin depends on Drp1 activity. (elsevier.com)
- 2013. Rapid nucleotide exchange renders asp-11 mutant actins resistant to depolymerizing activity of cofilin, leading to dominant toxicity in vivo. (cytoskeleton.com)
- This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. (bio-rad-antibodies.com)
- demonstrate that subunits in F-actin are capable of binding two ADF molecules, and that the depolymerization activity of ADF/cofilin may be due to the ability to induce cooperative changes in the tilt and twist of actin subunits. (rupress.org)
- In S. pombe , actin turnover is primarily regulated by the cofilin Adf1. (molbiolcell.org)
Microtubules2
- F-actin and microtubules (MTs) are the main mediators of neuronal polarity. (frontiersin.org)
- Two members of the tubulin superfamily, α- and β-tubulins are the building blocks for microtubules, which are polymerized and depolymerized dynamically. (molcells.org)
Myosin8
- Kovar DR. Intracellular motility: myosin and tropomyosin in actin cable flow. (uchicago.edu)
- [9] Tropomyosin inhibits the interaction between actin and myosin, while troponin senses the increase in calcium and releases the inhibition. (cloudfront.net)
- Motility by C. parvum sporozoites was prevented by latrunculin B and cytochalasin D, drugs that depolymerize the parasite actin cytoskeleton, and by the myosin inhibitor 2,3-butanedione monoxime. (omicsdi.org)
- Our studies demonstrate that C. parvum relies on a conserved actin-myosin motor for motility and active penetration of its host cell, thus establishing that this is a widely conserved feature of the Apicomplexa. (omicsdi.org)
- By using mutants of the fission yeast actin severing protein Adf1, we observed that contracting AMRs display a "peeling" phenotype, where bundles of actin and myosin peel off from one side of the AMR, and are pulled across to the opposite side. (molbiolcell.org)
- Visualizing AMR contraction face-on in adf1 -M2 and adf1 -M3 cells, we observed that bundles of myosin and (presumably) actin peeled off from one side of the AMR, and were pulled in toward the opposite side ( Figure 1A ), behavior that was not seen in wild-type (WT) cells ( Figure 1B ). (molbiolcell.org)
- What area of myosin associates with actin? (flashcardmachine.com)
- The myosin/actin interaction pulls heads inward and ATP hydrolysis drives contraction. (flashcardmachine.com)
Dynamics7
- Therefore, cucurbitacin I targets some factor involved in cellular actin dynamics other than actin itself. (nih.gov)
- These functional differences might be specifically adapted for different actin dynamics in muscle and non-muscle cells. (elsevier.com)
- In this review, we focus on recent developments regarding the role of actin in dendrite morphology, the regulation of actin dynamics by internal and external factors, and the role of F-actin in dendritic protein trafficking. (frontiersin.org)
- The focus of this review is the molecular mechanisms by which biophysical stimuli that induce changes in cytoplasmic actin dynamics are communicated within cells to elicit gene-specific transcription via nuclear localisation or activation of specialized transcription factors, namely MRTFs and the Hippo pathway effectors YAP and TAZ. (biomedcentral.com)
- Extracellular stimuli that alter actin dynamics are highly diverse and include soluble factors such as hormones and chemokines, or physical interactions between neighboring cells and the ECM. (biomedcentral.com)
- Since then, other factors that respond to and actively regulate actin dynamics have been identified. (biomedcentral.com)
- Rather this review will focus on how changes in cytoplasmic actin dynamics affect gene-specific transcription via nuclear localisation or activation of specialized transcription factors, namely MRTFs and the Hippo pathway effectors Yes-associated protein (YAP) and its paralog transcriptional coactivator with PDZ-binding motif (TAZ), in addition to some less characterised factors such as β-catenin, the NF-κB, Nrf2 and Foxj1a transcription factors, and epigenetic regulator HDAC3. (biomedcentral.com)
Disassembly2
- In in vitro actin depolymerization experiments, cucurbitacin I had no effect on the rate of actin filament disassembly at the nanomolar concentrations that inhibit cell migration. (nih.gov)
- These data indicate that the dynamic assembly and disassembly of F-actin on the mitochondria participates in Drp1-mediated mitochondrial fission. (elsevier.com)
Polymerization6
- Phalloidin increases F-actin microfilament content and actin-directed immunofluorescence in hepatocytes in vivo and also increases actin polymerization and the stability of F-actin in vitro. (unige.ch)
- We studied the sensitivity of immunofluorescent staining of actin to an actin depolymerizing factor (ADF) as well as actin content, degree of polymerization, and turnover in livers of in vivo phalloidin-treated rats. (unige.ch)
- They are involved in ACTIN polymerization and contain a polyproline-rich region that binds to PROFILIN, and a verprolin homology domain that binds G-ACTIN. (childrensmercy.org)
- It has hence become clear that tubulin straightening takes place during polymerization, whereas strongly kinked conformations are characteristic of depolymerized GDP-tubulin. (elifesciences.org)
- which blocks F-actin polymerization and results in AMR disintegration. (molbiolcell.org)
- How this occurs in the cell remained a mystery until the seminal discovery that actin polymerization is the trigger for nuclear localisation of myocardin-related transcription factor (MRTF) to stimulate serum response factor (SRF)-dependent transcription [ 3 ]. (biomedcentral.com)
Organization8
- They are also known to cause changes in the organization of the actin cytoskeleton. (nih.gov)
- During development of myocytes, gamma actin is thought to play a role in the organization and assembly of developing sarcomeres, evidenced in part by its early colocalization with alpha-actinin. (wikipedia.org)
- At the cellular level the alteration of Abl also changes actin organization consistent with RhoA inhibition. (jneurosci.org)
- In light of novel discoveries related to the role and organization of neuronal F-actin, in this review we will focus on the mechanisms and molecular players that fine-tune the actin cytoskeleton, thereby controlling dendrite morphology and function. (frontiersin.org)
- 2020). SPIN90 associates with mDia1 and the Arp2/3 complex to regulate cortical actin organization. (ijm.fr)
- Kadzik RS, Homa KE, Kovar DR. F-Actin Cytoskeleton Network Self-Organization Through Competition and Cooperation. (uchicago.edu)
- Newer models suggest the activity of actinomyosin cytoskeletal changes, extracellular matrix organization, intracellular signaling responses mediated by protein kinase C, Rho/Rho kinase, and other biologic factors in these processes. (touchophthalmology.com)
- Cells must therefore be able to sense the status of actin cytoskeleton organization and be able to communicate this to the cell nucleus to regulate gene transcription. (biomedcentral.com)
Microfilaments4
- Microfilaments are composed of the most abundant cellular protein known as actin. (cloudfront.net)
- Microfilaments are polymers of the protein actin and are 7 nm in diameter. (cloudfront.net)
- on the other hand, the contractile protein which is known as actin protein mainly produce microfilaments. (difference.wiki)
- on the other hand, microfilaments are mainly composed of a contractile protein called actin protein. (difference.wiki)
Appears to stabilize2
- Thus, phalloidin appears to stabilize actin against the depolymerizing actions of ADF, increases the proportion of F-actin without altering the size of the G-actin pool, and causes accumulation of actin by decreasing its relative rate of degradation. (unige.ch)
- Therefore, ADF appears to stabilize a preexisting F-actin conformation. (rupress.org)
Protein called actin1
- Both forms of the gelsolin protein attach (bind) to another protein called actin. (medlineplus.gov)
Capping actin protein1
- capping actin protein, gelsolin like. (gsea-msigdb.org)
Vitro1
- To this end, our team is developing experimental approaches combining microfluidics, micropatterning, and optical tweezers to conduct in vitro experiments that are essential to decipher the individual molecular reactions that regulate the emergence of actin networks. (ijm.fr)
Depolymerization2
- Moreover, UNC-60B induced much faster pointed-end depolymerization of rabbit muscle actin than UNC-60A, while UNC-60A caused slightly faster depolymerization of C. elegans actin than UNC-60B. (elsevier.com)
- The cooperative twist and tilt resulting from the binding of two molecules of ADF per actin subunit may lead to breakage of longitudinal contacts within the actin filament, and thus to F-actin depolymerization. (rupress.org)
Turnover5
- Twinfilin uncaps filament barbed ends to promote turnover of lamellipodial actin networks. (ijm.fr)
- The exact mechanisms that lead to this contractility are unknown, although some models posit that actin turnover in the AMR is essential. (molbiolcell.org)
- We found that the distribution of Myo2 in the AMR anticorrelates with the location of peeling events, suggesting that peeling is caused by a nonuniform tension distribution around the AMR, and that one of the roles of actin turnover is to maintain a uniform tension distribution around the AMR. (molbiolcell.org)
- We used adf1 mutants to investigate the effect of reduced actin turnover on AMR contraction and found that this causes a ring peeling phenotype, which seems to be due to a nonuniform distribution of tension around the AMR. (molbiolcell.org)
- We utilized two mutants of S. pombe Adf1, Adf1-M2 and Adf1-M3, which were shown to have reduced F-actin binding affinities and severing rates ( Chen and Pollard, 2011 ), causing reduced actin turnover in cells (Supplemental Figure S1A). (molbiolcell.org)
Rate of actin2
- Neither the relative fractional rate of actin synthesis nor its synthesis as a percent of total protein synthesis was altered either at one-day or ten-day post-phalloidin treatment. (unige.ch)
- Dualisotope experiments indicated that the rate of actin degradation was decreased selectively in the one- to three-day period following drug treatment. (unige.ch)
Role of actin1
- Yde SE, Kadzik RS, Kovar DR. Stir it up: The role of actin in mitochondrial mixing during mitosis. (uchicago.edu)
Roles of actin1
- So far, research has been focused on the specific roles of actin in the axon, while it is becoming more and more apparent that in the dendrite, actin is not only confined to dendritic spines, but serves many additional and important functions. (frontiersin.org)
Assembly of actin1
- Insertional assembly of actin filament barbed ends in association with formins produces piconewton forces. (uchicago.edu)
Complex3
- Development of the dendritic tree is a series of dynamic events that result in the formation of a complex and highly ordered structure through abundant remodeling and reorganization of the actin and microtubule cytoskeletons. (jneurosci.org)
- The 1-diphosphate for synthase of a activity illustrated for the extracellular factor( embryonic) ligases from the single complex predisposition Sphingomyelin at the Expression when a alternate latter acquiring a ultraviolet correlation area is metabolized on the nucleotide of the human syndrome: O&: filopodia repressor. (erik-mill.de)
- While the growth factors like insulin initiate a signaling cascade to induce conformational changes in the mechanistic target of rapamycin complex 1 (mTORC1), amino acids cause the complex to localize to the site of activation, the lysosome. (molcells.org)
Cytoplasmic1
- Actin, cytoplasmic 2, or gamma-actin is a protein that in humans is encoded by the ACTG1 gene. (wikipedia.org)
Fluorescent Actin1
- The fluorescent actin is seen to polymerize and depolymerize in cyclic waves. (ucsd.edu)
Cellular2
- The cellular effect of the compound is similar to that observed when cells are treated with the actin filament-stabilizing agent jasplakinolide. (nih.gov)
- In addition to established membrane remodeling roles in various cellular locations, actin has recently emerged as a participant in mitochondrial fission. (elsevier.com)
Gelsolin2
- It is thought that, through this function, the gelsolin protein regulates the formation of the actin cytoskeleton. (medlineplus.gov)
- Interactions of gelsolin and gelsolin-actin complexes with actin. (medlineplus.gov)
Rhodamine-phalloidin1
- Immunofluorescence assay was used to detect CpEF1α with a polyclonal antibody and host cell F-actin with rhodamine-phalloidin. (omicsdi.org)
Lattice2
- With the development of super-resolution microscopy in the past few years, previously unknown structures of the actin cytoskeleton have been uncovered: a periodic lattice consisting of actin and spectrin seems to pervade not only the whole axon, but also dendrites and even the necks of dendritic spines. (frontiersin.org)
- If a MT occasionally grows at a slower rate than GTP hydrolysis, the MT lattice depolymerizes rapidly. (elifesciences.org)
Phosphorylation2
- SASP Defects result in an regulator phosphorylation, depolymerizing the oxidative sensitivity( Kuilman et al. (erik-mill.de)
- The phosphorylated and activated S6K phosphorylates ribosomal protein S6 (RpS6), whereas 4E-BP1 phosphorylation by mTORC1 frees the eukaryotic initiation factor 4E from its negative regulator, 4E-BP1. (molcells.org)
Neuronal2
- Nonreceptor tyrosine kinase Abl is an actin-binding protein and a key regulator of neuronal axonal development. (jneurosci.org)
- Actin is a versatile and ubiquitous cytoskeletal protein that plays a major role in both the establishment and the maintenance of neuronal polarity. (frontiersin.org)
Elongation4
- transcription elongation factor, mit. (gsea-msigdb.org)
- Progressing actin: Formin as a processive elongation machine. (uchicago.edu)
- Cryptosporidium parvum Elongation Factor 1α Participates in the Formation of Base Structure at the Infection Site During Invasion. (omicsdi.org)
- We discovered that a C parvum translation elongation factor 1α (CpEF1α) was discharged from the invading sporozoites into host cells, forming a crescent-shaped patch that fully resembles the electron-dense band. (omicsdi.org)
Bind3
- pADF, unlike ADF, does not bind to G-actin, or affect the rate or extent of actin assembly. (rupress.org)
- Each single ABD, comprising two CH domains, is able to bind one actin monomer in the filament. (embl.de)
- The N-terminal CH domain has the intrinsic ability to bind actin, albeit with lower affinity than the complete ABD, whereas the C-terminal CH bind actin extremely weakly or not at all. (embl.de)
Subunits1
- The hydrolysis of ATP that occurs during this process is accompanied by a major change in the conformation of the actin subunits, and thus of the filament itself. (ijm.fr)
Nucleation2
- Effects of calcium on actin nucleation, filament severing, and end blocking. (medlineplus.gov)
- Mechanism of actin filament nucleation. (uchicago.edu)
Cell motility1
- The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. (wikipedia.org)
Bundles2
- Apart from that striking feature, patches of F-actin and deep actin filament bundles have been described along the lengths of neurites. (frontiersin.org)
- The LaminAC antibody specifically labels the nuclear lamina, while the actin antibody stains the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites. (immunologicalsciences.com)
Motility1
- Upon treatment of MDCK or B16-F1 cells with cucurbitacin I, there is a very rapid cessation of motility and gradual accumulation of filamentous actin aggregates. (nih.gov)
Conformation1
- Indeed, in response to oxidative stress due to its conformation changes, PARK7 releases these transcription factors, allowing them to translocate into the nucleus and to induce the expression of stress-response elements [ 11 , 12 ]. (hindawi.com)
Molecular2
- Human gamma-actin is 41.8 kDa in molecular weight and 375 amino acids in length. (wikipedia.org)
- Kovar DR. Molecular details of formin-mediated actin assembly. (uchicago.edu)
Gene1
- Actin, gamma 1, encoded by this gene, is found in non-muscle cells in the cytoplasm, and in muscle cells at costamere structures, or transverse points of cell-cell adhesion that run perpendicular to the long axis of myocytes. (wikipedia.org)
Destrin1
- destrin, actin depolymerizing fact. (gsea-msigdb.org)
Profilin1
- Control of the assembly of ATP- and ADP-actin by formins and profilin. (uchicago.edu)
Nuclear2
- nuclear factor I C [Source:HGNC Sy. (gsea-msigdb.org)
- Nuclear actin and its functional implication for general transcriptional activity will not be discussed here in detail. (biomedcentral.com)
Kinase1
- Among others, these include the actinomyosin cytoskeletal system, extracellular matrix, intracellular signaling responses mediated by protein kinase C, Rho/Rho kinase, and other biologic factors. (touchophthalmology.com)
Fission4
- We report that transient de novo F-actin assembly on the mitochondria occurs upon induction of mitochondrial fission and F-actin accumulates on the mitochondria without forming detectable submitochondrial foci. (elsevier.com)
- Li Y, Christensen JR, Homa KE, Hocky GM, Fok A, Sees JA, Voth GA, Kovar DR. The F-actin bundler a-actinin Ain1 is tailored for ring assembly and constriction during cytokinesis in fission yeast. (uchicago.edu)
- Scott BJ, Neidt EM, Kovar DR. The functionally distinct fission yeast formins have specific actin-assembly properties. (uchicago.edu)
- Kovar DR, Sirotkin V, Lord M. Three's company: the fission yeast actin cytoskeleton. (uchicago.edu)
Transcription factors3
- PARK7 as an oxidative sensor stabilizes transcription factors, including NRF2 and P53 in an oxidation-dependent manner. (hindawi.com)
- Filamins are involved in cell adhesion, spreading, and migration, acting as scaffolds for over 90 binding partners including channels, receptors, intracellular signaling molecules and transcription factors. (nih.gov)
- 2008). These genes frequently encode developmentally important transcription factors and are designated with a bivalent chromatin signature defined by the simultaneous presence of H3K4me3 and H3K27me3 (Azuara et al. (molecularcircuit.com)
Reorganization1
- Signaling pathways involving the Rho family of small GTPases mediate distinct actin cytoskeleton reorganization events in different cell types and have been proposed to be key mediators of dendritic development. (jneurosci.org)