Acriflavine: 3,6-Diamino-10-methylacridinium chloride mixt. with 3,6-acridinediamine. Fluorescent dye used as a local antiseptic and also as a biological stain. It intercalates into nucleic acids thereby inhibiting bacterial and viral replication.AcridinesPyronine: Xanthene dye used as a bacterial and biological stain. Synonyms: Pyronin; Pyronine G; Pyronine Y. Use also for Pyronine B. which is diethyl-rather than dimethylamino-.Bromouracil: 5-Bromo-2,4(1H,3H)-pyrimidinedione. Brominated derivative of uracil that acts as an antimetabolite, substituting for thymine in DNA. It is used mainly as an experimental mutagen, but its deoxyriboside (BROMODEOXYURIDINE) is used to treat neoplasms.Ethidium: A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide.Thiouracil: Occurs in seeds of Brassica and Crucifera species. Thiouracil has been used as antithyroid, coronary vasodilator, and in congestive heart failure although its use has been largely supplanted by other drugs. It is known to cause blood dyscrasias and suspected of terato- and carcinogenesis.Spheroplasts: Cells, usually bacteria or yeast, which have partially lost their cell wall, lost their characteristic shape and become round.Anti-Infective Agents, Local: Substances used on humans and other animals that destroy harmful microorganisms or inhibit their activity. They are distinguished from DISINFECTANTS, which are used on inanimate objects.Azaguanine: One of the early purine analogs showing antineoplastic activity. It functions as an antimetabolite and is easily incorporated into ribonucleic acids.NitrosoguanidinesErysipelothrix: A genus of gram-positive, facultatively anaerobic, rod-shaped bacteria that has a tendency to form long filaments. Its organisms are widely distributed in nature and are found in MAMMALS; BIRDS; and FISHES. Erysipelothrix may appear gram-negative because they decolorize easily.Aminoacridines: Acridines which are substituted in any position by one or more amino groups or substituted amino groups.Trypanosoma: A genus of flagellate protozoans found in the blood and lymph of vertebrates and invertebrates, both hosts being required to complete the life cycle.Drug Resistance, Microbial: The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).Genetics, Microbial: A subdiscipline of genetics which deals with the genetic mechanisms and processes of microorganisms.Serratia marcescens: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria found in soil, water, food, and clinical specimens. It is a prominent opportunistic pathogen for hospitalized patients.Antiporters: Membrane transporters that co-transport two or more dissimilar molecules in the opposite direction across a membrane. Usually the transport of one ion or molecule is against its electrochemical gradient and is "powered" by the movement of another ion or molecule with its electrochemical gradient.Membrane Transport Proteins: Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.Water: A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Intestinal Diseases, Parasitic: Infections of the INTESTINES with PARASITES, commonly involving PARASITIC WORMS. Infections with roundworms (NEMATODE INFECTIONS) and tapeworms (CESTODE INFECTIONS) are also known as HELMINTHIASIS.Parasitic Diseases: Infections or infestations with parasitic organisms. They are often contracted through contact with an intermediate vector, but may occur as the result of direct exposure.Salmonella typhimurium: A serotype of Salmonella enterica that is a frequent agent of Salmonella gastroenteritis in humans. It also causes PARATYPHOID FEVER.Salmonella enterica: A subgenus of Salmonella containing several medically important serotypes. The habitat for the majority of strains is warm-blooded animals.Salmonella Infections, Animal: Infections in animals with bacteria of the genus SALMONELLA.Salmonella Infections: Infections with bacteria of the genus SALMONELLA.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Bacterial Proteins: Proteins found in any species of bacterium.Crithidia: A genus of parasitic protozoans found in the digestive tract of invertebrates, especially insects. Organisms of this genus have an amastigote and choanomastigote stage in their life cycle.Trypanosomatina: A suborder of monoflagellate parasitic protozoa that lives in the blood and tissues of man and animals. Representative genera include: Blastocrithidia, Leptomonas, CRITHIDIA, Herpetomonas, LEISHMANIA, Phytomonas, and TRYPANOSOMA. Species of this suborder may exist in two or more morphologic stages formerly named after genera exemplifying these forms - amastigote (LEISHMANIA), choanomastigote (CRITHIDIA), promastigote (Leptomonas), opisthomastigote (Herpetomonas), epimastigote (Blastocrithidia), and trypomastigote (TRYPANOSOMA).DNA, Kinetoplast: DNA of kinetoplasts which are specialized MITOCHONDRIA of trypanosomes and related parasitic protozoa within the order KINETOPLASTIDA. Kinetoplast DNA consists of a complex network of numerous catenated rings of two classes; the first being a large number of small DNA duplex rings, called minicircles, approximately 2000 base pairs in length, and the second being several dozen much larger rings, called maxicircles, approximately 37 kb in length.Centrifugation: Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Fish Diseases: Diseases of freshwater, marine, hatchery or aquarium fish. This term includes diseases of both teleosts (true fish) and elasmobranchs (sharks, rays and skates).Flexibacter: A genus of gram-negative, chemoorganotrophic bacteria in the family CYTOPHAGACEAE. In some species there is a cyclic change in cell morphology.Fishes: A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.Rosaniline Dyes: Compounds that contain the triphenylmethane aniline structure found in rosaniline. Many of them have a characteristic magenta color and are used as COLORING AGENTS.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Protozoan Infections: Infections with unicellular organisms formerly members of the subkingdom Protozoa.Biological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Workplace: Place or physical location of work or employment.Uncertainty: The condition in which reasonable knowledge regarding risks, benefits, or the future is not available.Electronic Mail: Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.Disease Outbreaks: Sudden increase in the incidence of a disease. The concept includes EPIDEMICS and PANDEMICS.Diazonium CompoundsAzo CompoundsPelvic Floor: Soft tissue formed mainly by the pelvic diaphragm, which is composed of the two levator ani and two coccygeus muscles. The pelvic diaphragm lies just below the pelvic aperture (outlet) and separates the pelvic cavity from the PERINEUM. It extends between the PUBIC BONE anteriorly and the COCCYX posteriorly.Constipation: Infrequent or difficult evacuation of FECES. These symptoms are associated with a variety of causes, including low DIETARY FIBER intake, emotional or nervous disturbances, systemic and structural disorders, drug-induced aggravation, and infections.Myenteric Plexus: One of two ganglionated neural networks which together form the ENTERIC NERVOUS SYSTEM. The myenteric (Auerbach's) plexus is located between the longitudinal and circular muscle layers of the gut. Its neurons project to the circular muscle, to other myenteric ganglia, to submucosal ganglia, or directly to the epithelium, and play an important role in regulating and patterning gut motility. (From FASEB J 1989;3:127-38)Psychological Techniques: Methods used in the diagnosis and treatment of behavioral, personality, and mental disorders.Diazepam: A benzodiazepine with anticonvulsant, anxiolytic, sedative, muscle relaxant, and amnesic properties and a long duration of action. Its actions are mediated by enhancement of GAMMA-AMINOBUTYRIC ACID activity.IndiaMolluginaceae: A plant family of the order Caryophyllales, subclass Caryophyllidae, class Magnoliopsida. Some members contain triterpenoid saponins.Drug Industry: That segment of commercial enterprise devoted to the design, development, and manufacture of chemical products for use in the diagnosis and treatment of disease, disability, or other dysfunction, or to improve function.

Bacteriophage T4 rnh (RNase H) null mutations: effects on spontaneous mutation and epistatic interaction with rII mutations. (1/109)

The bacteriophage T4 rnh gene encodes T4 RNase H, a relative of a family of flap endonucleases. T4 rnh null mutations reduce burst sizes, increase sensitivity to DNA damage, and increase the frequency of acriflavin resistance (Acr) mutations. Because mutations in the related Saccharomyces cerevisiae RAD27 gene display a remarkable duplication mutator phenotype, we further explored the impact of rnh mutations upon the mutation process. We observed that most Acr mutants in an rnh+ strain contain ac mutations, whereas only roughly half of the Acr mutants detected in an rnhDelta strain bear ac mutations. In contrast to the mutational specificity displayed by most mutators, the DNA alterations of ac mutations arising in rnhDelta and rnh+ backgrounds are indistinguishable. Thus, the increase in Acr mutants in an rnhDelta background is probably not due to a mutator effect. This conclusion is supported by the lack of increase in the frequency of rI mutations in an rnhDelta background. In a screen that detects mutations at both the rI locus and the much larger rII locus, the r frequency was severalfold lower in an rnhDelta background. This decrease was due to the phenotype of rnh rII double mutants, which display an r+ plaque morphology but retain the characteristic inability of rII mutants to grow on lambda lysogens. Finally, we summarize those aspects of T4 forward-mutation systems which are relevant to optimal choices for investigating quantitative and qualitative aspects of the mutation process.  (+info)

Scanning cysteine accessibility of EmrE, an H+-coupled multidrug transporter from Escherichia coli, reveals a hydrophobic pathway for solutes. (2/109)

EmrE is a 12-kDa Escherichia coli multidrug transporter that confers resistance to a wide variety of toxic reagents by actively removing them in exchange for hydrogen ions. The three native Cys residues in EmrE are inaccessible to N-ethylmaleimide (NEM) and a series of other sulfhydryls. In addition, each of the three residues can be replaced with Ser without significant loss of activity. A protein without all the three Cys residues (Cys-less) has been generated and shown to be functional. Using this Cys-less protein, we have now generated a series of 48 single Cys replacements throughout the protein. The majority of them (43) show transport activity as judged from the ability of the mutant proteins to confer resistance against toxic compounds and from in vitro analysis of their activity in proteoliposomes. Here we describe the use of these mutants to study the accessibility to NEM, a membrane permeant sulfhydryl reagent. The study has been done systematically so that in one transmembrane segment (TMS2) each single residue was replaced. In each of the other three transmembrane segments, at least four residues covering one turn of the helix were replaced. The results show that although the residues in putative hydrophilic loops readily react with NEM, none of the residues in putative transmembrane domains are accessible to the reagent. The results imply very tight packing of the protein without any continuous aqueous domain. Based on the findings described in this work, we conclude that in EmrE the substrates are translocated through a hydrophobic pathway.  (+info)

Candida albicans mutants deficient in respiration are resistant to the small cationic salivary antimicrobial peptide histatin 5. (3/109)

Histatins are a group of small cationic peptides in human saliva which are well known for their antibacterial and antifungal activities. In a previous study we demonstrated that histatin 5 kills both blastoconidia and germ tubes of Candida albicans in a time- and concentration-dependent manner at 37 degrees C, whereas no killing was detected at 4 degrees C. This indicated that killing activity depends on cellular energy. To test histatin 5 killing activity at lower cellular ATP levels at 37 degrees C, respiratory mutants, or so-called petite mutants, of C. albicans were prepared. These mutants are deficient in respiration due to mutations in mitochondrial DNA. Mutants were initially identified by their small colony size and were further characterized with respect to colony morphology, growth characteristics, respiratory activity, and cytochrome spectra. The killing activity of histatin 5 at the highest concentration was only 28 to 30% against respiratory mutants, whereas 98% of the wild-type cells were killed. Furthermore, histatin 5 killing activity was also tested on wild-type cells in the presence of the respiratory inhibitor sodium azide or, alternatively, the uncoupler carbonyl cyanide m-chlorophenylhydrazone. In both cases histatin 5 killing activity was significantly reduced. Additionally, supernatants and pellets of cells incubated with histatin 5 in the presence or absence of inhibitors of mitochondrial ATP synthesis were analyzed by sodium dodecyl sulfate gel electrophoresis. It was observed that wild-type cells accumulated large amounts of histatin 5, while wild-type cells treated with inhibitors or petite mutants did not accumulate significant amounts of the peptide. These data showed first that cellular accumulation of histatin 5 is necessary for killing activity and second that accumulation of histatin 5 depends on the availability of cellular energy. Therefore, mitochondrial ATP synthesis is required for effective killing activity of histatin 5.  (+info)

An essential glutamyl residue in EmrE, a multidrug antiporter from Escherichia coli. (4/109)

EmrE is an Escherichia coli 12-kDa protein that confers resistance to toxic compounds, by actively removing them in exchange with protons. The protein includes eight charged residues. Seven of these residues are located in the hydrophilic loops and can be replaced with either Cys or another amino acid bearing the same charge, without impairing transport activity. Glu-14 is the only charged residue in the membrane domain and is conserved in all the proteins of the family. We show here that this residue is the site of action of dicyclohexylcarbodiimide, a carbodiimide known to act in hydrophobic environments. When Glu-14 was replaced with either Cys or Asp, resistance was abolished. Whereas the E14C mutant displays no transport activity, the E14D protein shows efflux and exchange at rates about 30-50% that of the wild type. The maximal DeltapH-driven uptake rate of E14D is only 10% that of the wild type. The mutant shows a different pH profile in all the transport modes. Our results support the notion that Glu-14 is an essential part of a binding domain shared by substrates and protons but mutually exclusive in time. This notion provides the molecular basis for the obligatory exchange catalyzed by EmrE.  (+info)

A two-component multidrug efflux pump, EbrAB, in Bacillus subtilis. (5/109)

Genes (ebrAB) responsible for ethidium resistance were cloned from chromosomal DNA of Bacillus subtilis ATCC 9372. The recombinant plasmid produced elevated resistance against ethidium bromide, acriflavine, pyronine Y, and safranin O not only in Escherichia coli but also in B. subtilis. It also caused an elevated energy-dependent efflux of ethidium in E. coli. EbrA and EbrB showed high sequence similarity with members of the small multidrug resistance (SMR) family of multidrug efflux pumps. Neither ebrA nor ebrB was sufficient for resistance, but introduction of the two genes carried on different plasmids conferred drug resistance. Thus, both EbrA and EbrB appear to be necessary for activity of the multidrug efflux pump. In known members of the SMR family, only one gene produces drug efflux. Thus, EbrAB is a novel SMR family multidrug efflux pump with two components.  (+info)

Ozonation of mutagenic and carcinogenic polyaromatic amines and polyaromatic hydrocarbons in water. (6/109)

The Salmonella-microsome assay for mutagenesis was used to determine the effect of ozone on the mutagenesis of selected carcinogens and mutagens in water. Short periods of ozonation were shown to completely inactivate the mutagenicity of several polyaromatic amine mutagens including acriflavine, proflavine, and beta-naphthylamine. Selected polyaromatic hydrocarbons were also sensitive to ozonation. Kinetic studies revealed that the mutagenicity of benzo(a)pyrene, 3-methylcholanthrene, and 7,12-dimethylbenz(a)anthracene was destroyed after short periods of ozonation. To correlate loss of mutagenicity with loss of carcinogenicity, two polyaromatic hydrocarbons were treated with ozone, extracted from water with hexane, and tested for carcinogenicity in mice. When 7,12-dimethyl-benz(a)anthracene and 3-methyl-cholanthrene were treated with ozone, there was a substantial reduction in carcinogenicity compared to control groups treated with oxygen alone. However, a small number of tumors developed in the group of animals receiving a hexane extract of ozonated 7,12-dimethylbenz(a)anthracene. This activity may be due to breakdown products of 7,12-dimethylbenz(a)anthracene that are not mutagenic.  (+info)

Interaction of the expression of two membrane genes, acrA and plsA, in Escherichia coli K-12. (7/109)

The mutation acrA1, leading to acriflavine sensitivity through disorganization of the plasma membrane, is located between proC and purE on the Escherichia coli K-12 chromosome. Gene plsA has been reported to determine biosynthesis of membrane phospholipid and to be located very near acrA (1). Genes acrA and plsA fall into different cistrons and are arranged in the order proC-acrA-plasA-purE. The genes were shown to interact with each other. Introduction of acrA mutation into a plsA temperature-sensitive mutant mitigated the heat sensitivity. Plasmid (F-gal+) stability in acrA mutants was restored by introduction of the plasA mutation into the acrA cells. When an Hfr plsA donor was conjugated with an acrA recipient, or when reciprocally conjugated, the exogenotes were eliminated at high frequency during subsequent subcultivation in broth. However, the exogenotes were not eliminated in all other allelic combinations of genes acrA and plsA. When an F-gal+ plasmid was introduced into the unstable heterozygotes (acrA+plsA/acrApls1+), the plasmids were stably hosted, whereas the acrA+ plasA exogenotes were spontaneously lost at a high frequency. On the other hand, when the unstable heterozygotes carrying F-gal+ were cultured in acriflavine-containing medium, the F-gal+ plasmids were preferentially eliminated but the acrA+plasA exogenotes were not affected. The results suggest that the organization of the plasma membrane controls the recombination of the exogenotes introduced into zygotes.  (+info)

Role of pyrimidine dimer excision in loss of potential streptomycin resistance mutations of ultraviolet-irradiated Escherichia coli on phosphate-buffered agar. (8/109)

The frequency of ultraviolet (UV)-induced mutations to streptomycin resistance dropped rapidly when starved Escherichia coli strains WP-2 B/r and B/r T- were incubated on phosphate-buffered agar (PBA), but was reduced only slightly in a WP-2 hcr- mutant. During postirradiation, incubation viability remained approximately constant. Cells given an optimal recovery treatment with photo-reactivating light showed no further recovery if subsequently incubated on PBA. At least 70% of the mutations induced to streptomycin resistance by UV could be repaired. The loss of potential streptomycin-resistant mutants was markedly reduced in strain B/r T- when 5 mug of acriflavin or 700 mug of caffeine per ml was added to PBA. The excision of UV-induced thymine-containing dimers from E. coli tb/r T- was investigated. Dimer excision progressed more slowly when the cells were incubated on PBA containing acriflavin or caffeine. There was no congruity between the kinetics of dimer excision and the kinetics of mutant loss. Our results indicate that removal of potential streptomycin-resistant mutants is considerably faster than the excision of pyrimidine dimers.  (+info)

The unexpected small reductions in mutation frequencies between Selfed Generations 1 and 2 for ascospore color did not occur for acriflavine resistance, so it may not be a general effect. There were even some increases in mutation frequency for acriflavine resistance between Selfed Generations 1 and 2 within each slope, for example, 1997 data, 250 μg/ml, NFS (P , 0.05), but most were not significant.. Stability of mutation frequencies and of mutations: Although mutations arising at different times during colony growth could account for some heterogeneity between repeats or replicates, the fact that most replicates and repeats were homogenous for mutation frequencies suggests that this phenomenon had little effect on within-slope or between-slope results, especially as between-slope differences were consistent across all three repeats.. In this colonial fungus, there are many millions of nuclei in one Petri dish culture, and thousands even in a fragment of one hypha. A vegetative subculture will ...
Acriflavine is a preventative and treatment for the control of Oodinium (Velvet) in freshwater and marine fishes. It is also used as treatment for external fungal infections of aquarium fish. It has a long history of usefulness in the control of fish diseases. Acriflavine is useful for fish egg disinfection, treatment of open wounds, and external protozoan infections. The chemical said to be effective in the control of the bacterial infection columnaris (Flexibacter columnaris). But is not usually applied for other bacterial infections. Acriflavine may also be used as an alternative to Malachite Green when it is known that certain species of fish may be sensitive (small Characins). ...
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Short Duration Bath (2 hours) - Using an independent, separate treatment container with appropriate aeration, prepare as 10 part per million (ppm) treatment bath. Note: 10 ppm is equivalent to 10 milligrams of acriflavine per liter of treatment water or 37.8 mg per gallon of treatmet water. After 2 hours of treatment, return fish to habitat water ...
SHREEJI PHARMA International based in Gujarat, India is a manufacturer, exporter & trader of Acriflavine Neutral BPC-49 (Euflavine) at the best price.
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Constipation may reflect both peripheral involvement, indicated by Lewy bodies in the colonic myenteric plexus, leading to colonic inertia, and central mechanisms, leading to pelvic floor dysfunction. Also the peak tailing was substantially diminished, giving an ability to separate DNA adducts equal to or better than the TLC method. Individuals with asthma often have comorbid mental health conditions, the rates of which are also marked by significant racial and ethnic disparities. The performance of a chemoselective addition of diheteroarylzincs to aryldiazonium salts allows an efficient preparation of new heterocyclic azo compounds. These direct vascular effects are related to vascular tissue levels of cheap viagra online canada 5-methyltetrahydrofolate rather than plasma levels. Treatment discontinuation with peg-interferon: what to consider. AL amyloidosis may be associated locally with pulmonary lymphoma. Mock-ups of 15 prototype canada pharmacy viagra online features were developed based on ...
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CP001069.PE445 Location/Qualifiers FT CDS_pept complement(507044..510250) FT /codon_start=1 FT /transl_table=11 FT /locus_tag="Rpic_4224" FT /product="transporter, hydrophobe/amphiphile efflux-1 FT (HAE1) family" FT /note="TIGRFAM: transporter, hydrophobe/amphiphile efflux-1 FT (HAE1) family; PFAM: acriflavin resistance protein; KEGG: FT rso:RSp1113 probable transmembrane multidrug-efflux system FT lipoprotein transmembrane" FT /db_xref="EnsemblGenomes-Gn:Rpic_4224" FT /db_xref="EnsemblGenomes-Tr:ACD29321" FT /db_xref="GOA:B2UIC0" FT /db_xref="InterPro:IPR000731" FT /db_xref="InterPro:IPR001036" FT /db_xref="InterPro:IPR004764" FT /db_xref="InterPro:IPR027463" FT /db_xref="UniProtKB/TrEMBL:B2UIC0" FT /protein_id="ACD29321.1" FT /translation="MNFSKFFVDRPIFAGVLSTLILLIGIISIWRLPISEYPEVVPPSV FT VVRAQFPGANPKVIAETVASPLEEQINGVENMLYMQSQANSDGNLTTTVTFKLGTDPDK FT AQQLVQNRVSQALPRLPDVVQRLGVTTVKSSPDLTMVVHLLSPNDRYDMTYLRNYAVLN FT VKDRLARISGVGQVQLFGSGDYSMRVWLDPNKVAERGLTANEVVKAIRDQNVQVAAGVI FT ...
There are 3 twelve-letter words containing 2A, F, I, L, N and V: ACRIFLAVINES LACTOFLAVINS & TRYPAFLAVINE. Every word on this site is valid scrabble words. Build other lists, beginning with or ending with letters of your choice.
The effects of acriflavine on two species of Trypanosomatidae, Crithidia luciliae and Trypanosoma mega, have been investigated. It has been observed that kinetoplastic (i.e. mitochondrial) DNA is lost in a high percentage of acriflavine-treated cells. Resting flagellates, from stationary-phase or hemin-deficient cultures, are considerably more resistant to the acridine than are flagellates from a log-phase culture. When the kinetoplast has retained some DNA and still remains visible in stained smears, it appears reduced in size, and its ultrastructure is extremely abnormal: the DNA fibrils, clearly visible in normal kinetoplasts, are condensed; they appear as an electron-opaque, apparently homogeneous mass, separated from the membranes by a space of low electron-opacity. Analyses of DNA extracts, with high speed centrifugation in CsCl density gradients, revealed that the satellite band, presumably kinetoplastic DNA, is lost by trypanosomes grown for 5 days in the presence of acriflavine. ...
0145] A wide variety of fluorescent molecules can be utilized in the present invention including small molecules, fluorescent proteins and quantum dots. Useful fluorescent molecules (fluorophores) include, but are not limited to: 1,5 IAEDANS; 1,8-ANS; 4-Methylumbelliferone; 5-carboxy-2,7-dichlorofluorescein; 5-Carboxyfluorescein (5-FAM); 5-Carboxynapthofluorescein; 5-Carboxytetramethylrhodamine (5-TAMRA); 5-FAM (5-Carboxyfluorescein); 5-HAT (Hydroxy Tryptamine); 5-Hydroxy Tryptamine (HAT); 5-ROX (carboxy-X-rhodamine); 5-TAMRA (5-Carboxytetramethylrhodamine); 6-Carboxyrhodamine 6G; 6-CR 6G; 6-JOE; 7-Amino-4-methylcoumarin; 7-Aminoactinomycin D (7-AAD); 7-Hydroxy-4-methylcoumarin; 9-Amino-6-chloro-2-methoxyacridine; ABQ; Acid Fuchsin; ACMA (9-Amino-6-chloro-2-methoxyacridine); Acridine Orange; Acridine Red; Acridine Yellow; Acriflavin; Acriflavin Feulgen SITSA; Aequorin (Photoprotein); AFPs--AutoFluorescent Protein--(Quantum Biotechnologies); Alexa Fluor 350®; Alexa Fluor 430®; Alexa Fluor ...
The multidrug (chloramphenicol, tetracycline, norfloxacin, doxorubicin, trimethoprim, acriflavin, ethidium bromide, tetraphenylphosphonium, TPP, benzalkonium, ciprofloxacin, thiamphenicol, IPTG) resistance exporter, MdfA (catalyzes both electrogenic and electroneutral transport) (Adler and Bibi, 2004). Can function as a Na+ (K+)/H+ antiporter (Lewinson and Bibi 2001; Higgins, 2007). Is known to provide resistance to a wide variety of dissimilar toxic compounds, including neutral, cationic and zwitterionic substances. Crystals that diffracted to 3.4 Å resolution and belonged to the hexagonal space group P6122 have been obtained (Nagarathinam et al. 2017). For review of MdfA see Lewinson et al., 2006. The conformational switch accompanying transport is induced by promiscuous binding of substrates and/or inhibitors to the binding pocket (Fluman et al., 2009). Normally extrudes monovalent cationic drugs in exchange for a single proton, but it transports divalent cationic drugs poorly. It can be ...
Ozone is the ONLY single method we have of destroying free-swimming parasites, bacterium and viruses. It also literally burns up organics and phenols. Due to the relatively short life span of ozone it quickly reverts to oxygen enriching the water quality. Obviously, improved water quality combined with the high oxygen content and the lack of bacteria to cause primary and secondary infections gives a huge boost to the livestocks own immune system enabling speedy recover from knocks, parasite infestation, bacterial infections. Another major benefit is the prevention of cross infection in centralised systems. Systems using ozone generators do not require regular dosing of medications such as malachite green, formaline, acriflavine or antibiotics. If they were to be administered the ozone generated would burn them out. Why do we need a ORP Controller/Meter and Probe? The ORP uses a probe, which is normally situated in the system reservoir to continually monitor and provide an accurate and. ...
Hi, I have tried without luck to cure the growth on my goldfish but with no luck. Now that the growth on my fish has grown I have better quality pics that may help diagnosis and treatment. Could I please ask for help and advice? I have tried MultiCure a couple of times over the last few months (has Malachite Green, Methylene Blue, Acriflavine) and more recently I tried Melafix. Nothing seems to have worked. Im always scared of overdosing medications so maybe I didnt use enough or for as long as
The presence of putative ebrAB genes in the chromosomal DNA of B. subtilis has been revealed by whole-genome sequencing (9). Judging from the similarity of the deduced amino acid sequences between EbrA or EbrB and members of SMR family of multidrug efflux proteins, it seemed that the EbrAB is responsible for ethidium bromide resistance. We cloned the ebrAB genes fromB. subtilis ATCC 9372 and expressed them in E. coli cells. In fact, our data suggest that the EbrAB is a multidrug efflux pump and is involved in multidrug resistance against cationic lipophilic dyes such as ethidium bromide, acriflavine, pyronine Y, and safranin O. Three signature sequences that are specific to the SMR family members (19) were all present in deduced amino acid sequences of EbrA and EbrB (data not shown). Thus, we believe that the EbrAB is a member of the SMR family.. Each member of the SMR family so far reported is encoded by a single gene. Smr of S. aureus (6) and EmrE of E. coli (25) have been purified to ...
Several efflux proteins are well described in gram-positive bacteria, including Bmr in Bacillus subtilis (1) and NorA in Staphylococcus aureus (15). These can mediate low-level resistance to hydrophilic fluoroquinolones and a variety of unrelated compounds; both are inhibited by reserpine (14, 15). More recently, reserpine was shown to inhibit the level of accumulation of ethidium bromide in an ethidium bromide-selected mutant of S. pneumoniae which showed cross-resistance to fluoroquinolones (5). For this reason we used reserpine to detect mutants resistant to fluoroquinolones by an efflux mechanism.. We showed that reserpine significantly increased the activity of norfloxacin against several norfloxacin-selected mutants. These presumptive efflux mutants also showed increased levels of resistance to ethidium bromide and acriflavine, which agrees with the description by Zeller et al. (20) of the presence of an efflux pump in pneumococci. Ethidium bromide accumulation in strain 1N27, a ...
Extra Strength Fungidye(tm) patented formula Made for Hair infection, Hoof Rot and fungus. Penetrates into all infected cavities. It is insoluble in water or urine. The anti-fungal agents, and two anti-bacterial agents stay after covering up. It does not kill tissue. For use as an antiseptic and wound-healing agent. Also for use as a diagnostic tool to locate cavities during resection. It is clinicly proven to be used as a topical treatment or under a patch repair. A proven preventative from fungus infection. Ingredients: Chemotherapeutic agents, Organic trypanocide dyes. It does not contain formaldehyde or copper sulfate. Acriflavine HCL, Gentian Violet. Use in nail holes and affected areas. It gives dormant organisms an opportunity to grow healthy. It stops the growth of the fungi ...
hey guy here is a formula that i just cant figure out if anyone can break it down better, i would appreciate a reply. 44,000 sqf = 1 acre...
Mustafa etin, Sinan Altan Kocaman, Turan Erdo an, Murtaza Emre Durako lugil, Y ksel i ek, ahin Bozok, Aytun anga, Ahmet Temiz, S tk Do an, mer at ro ...
Hydrocyanicum Acidum materia medica indications. Buy Hydrocyanicum Acidum the homeopathic remedy in 8X-200X, 4C-200C, 1M-10M, CM
Learn about the veterinary topic of Overview of |i |Erysipelothrix rhusiopathiae|/i| Infection. Find specific details on this topic and related topics from the Merck Vet Manual.
I am growing MCF-7 cell line. I have been trying to stain dead cells using ethidium bromide and I havnt been very successful. I found the protocol online but it doesnt seem to work. if there is any other method with which I can stain the cells please let me know. Thanks in advance ...
Does anyone know the mechanism(s) of mutagenesis by ethidium bromide? Any references? Thanks. Chris Cole U. of Minn-Morris Morris, MN 56267 colect at caa.mrs.umn.edu ...
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
Chip Flory. From The Editor. June 29, 2012. Hello Pro Farmer Members!. Its not very often the June Acreage Report from USDA can add 2.315 million acres to expected soybean harvested acres and the market can close higher. Planted soybean acres were about 500,000 above the average pre-report trade estimate, so the harvested acreage number blew-away trade expectations, as well.. Nonetheless, soybean futures charged higher again today. Without stress-relieving rains in the near-term, the contract high in November soybeans set earlier this week will likely get pushed aside next week.. The counter to the bigger-than-expected increase in soybean acres is a declining yield estimate. In this weeks Pro Farmer, we stayed conservative with our national average soybean yield assumption and put it at 43 bu. per acre. Thats down 0.9 bu. from USDAs projection in the June S&D Report, but its above many crop-watchers yield peg. One of those lower estimates is reportedly coming from Informa Economics. Source ...
BondEX Ethidium Bromide Detoxification Cartridges safely remove ethidium bromide as well as SYBR Green from electrophoresis buffers or other aqueous solutions.Easy-to-use, gravity-flow cartridges
BondEX Ethidium Bromide Detoxification Cartridges safely remove ethidium bromide as well as SYBR Green from electrophoresis buffers or other aqueous solutions.Easy-to-use, gravity-flow cartridges
View details of 13 Acre Turn Key Mini Farm. Farm for Sale with 13 acres by Ashville in St. Clair County, Alabama 35953 at FARMFLIP.com.
I always dilute 20 µl ethibro (10 mg/ml) in 400 ml water. this means, the concentration becomes 0.05 µg/ml ethibro solution (please correct me if im wrong ...
Clark & Laura Huinker. 759 State Hwy. 15. Fairmont, MN 56031. http://www.breezyacresboergoats.com Raising fullblood, percentage and commercial goats
Experiments at the University of Florida Tropical Aquaculture Laboratory were run to test various shipping chemical additives for their effects on appearance and behavior of fish post shipping. The chemicals tested were tricaine methanesulfonate (MS-222), salt (3 ppt NaCl), methylene blue, acriflavine neutral, quinaldine, eugenol, an experimental commercial product in the form of a feed, and a water additive.. The commercial product contained arachidonic acid (ArA), a fungus extract from Mortierella sp., claimed by the manufacturer to be used to regulate immune systems; and an ethanol extract from the genus Opuntia, a cactus species, claimed by the manufacturer to be used to stimulate the production of heat shock proteins (HSPs) that work to counteract stress responses.. Tricaine methanesulfonate is approved for use in finfish as a sedative and anesthetic. Quinaldine and eugenol are not approved for use in aquaculture. A description of approval for the other chemicals tested in this experiment ...
Erysipelothrix rhusiopathiae is a primary pathogen of swine and turkeys and sporadic cause of disease in a variety of other hosts, including humans. A genomic library of the highly virulent strain of E. rhusiopathiae E1-6P was constructed in the expression-cloning vector lambda gt11 and screened with serum from a pig convalescent from an E. rhusiopathiae experimental infection. Immunoreactive clones were screened for their ability to protectively immunized mice. Two clones, lambda gt11/ersA and lambda gt11/ersB, were obtained that protected mice against challenge with E. rhusiopathiae E1-6P. Antisera against the recombinant clones reacted with polypeptides of molecular weights 66,000, 64,000, and 43,000 in detergent-solubilized surface antigen preparations and whole-cell lysates of E. rhusiopathiae. These polypeptides were also the major antigens recognized by convalescent pig serum when reacted with the same preparations. Western immunoblot and Southern blot analysis revealed that the cloned ...
The causative organism of Erysipelas in pigs is the ubiquitous bacterium Erysipelothrix rhusiopathiae, formally known as Erysipelas insidiosa
P. N. Ashley, 855 acres, $30,000. It is safe to double the assessment when seeking the market value. Baird Bros., 1,118 acres, Woodland valley, $68,000. Olive J. Bandy, 5,894 acres, $54,620. Bullard Co., 1,661 acres, Woodland valley, $96,050. Capay Valley Land Co., 2,680 acres, $98,380. Ellen W. Coil, 2,030 acres, Woodland valley, $81,550. C. J. Day Estate, 1,893 acres, $20,824. H. P. Eakle, 1,025 acres, Wood Prairie, $46,040. Forbes Estate, 8,079 acres, Fairview, $16,700. H. H. Gable et al., 7,800 acres, $69,700. Eliza Gallup et al., at Willow Slough, Grafton, Fairview, 4,662 acres, $44,940. D. N. Hershey Estate, 15,477 acres, $290,607. G. W. Hollingsworth, 16,470 acres, $21,655. Mrs. J. E. Merritt, 3,605 acres, $152,150. T. A. Sparks, 2,341 acres, $21,045. A. W. Morris, 1,814 acres, $91,155. Sacramento River Farms Co., 10,283 acres, $140,000. Alice Tubbs, 5,715 acres, North Grafton, $43,755. Yolo Ranch Co, 2,055 acres, Grafton, $23,055. Agnes Beverly et al., 13,166 acres, Grafton, $205,240. ...
Acres Treated is the acreage of the crop actually treated with the pesticide. Gross Pounds applied divided by Acres Treated is the application rate of the pesticide. The difference between Acres Planted and Acres Treated is best explained through an example:If a farmer has a 100-acre field and sprays 50 acres, then the Acres Planted will be 100 and the Acres Treated will be 50. If a farmer sprays 50 of their 100 acres three times, then the Acres Planted will remain at 100 acres, but now the value of Acres Treated will be 150. This figure may not be the total acreage of the selected crop. See documentation for further distinctions between acres treated and acres planted ...
The town council Tuesday rezoned 20 acres at Kennedy Boulevard and Keller Road from residential to commercial use.An 8.7-acres tract lies on the west side of Keller Road and an 11.7-acre tract is on
The Whitewater-Baldy fire, the largest in New Mexico history, has grown an additional 20,000 acres since Wednesday to more than 190,000 acres, officials said.
Diagnosis. Based in Lehigh Acres, FL, Lehigh Regional Medical Center serves a five-county area, boasting a broad range of medical specialties.
18702 Oden Trace Dr Tomball TX 77377 was recently sold. It is a 0.19 Acre(s) Lot, 3,573 SQFT, 5 Beds, 4 Full Bath(s) & 1 Half Bath(s) in Wildwood/Northpointe.
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What is the difference between Erysipelas and Cellulitis? Lesions are localized and have clearly demarcated boundaries in erysipelas; in cellulitis, the lesions
Acres Treated is the acreage of the crop actually treated with the pesticide. Gross Pounds applied divided by Acres Treated is the application rate of the pesticide. The difference between Acres Planted and Acres Treated is best explained through an example:If a farmer has a 100-acre field and sprays 50 acres, then the Acres Planted will be 100 and the Acres Treated will be 50. If a farmer sprays 50 of their 100 acres three times, then the Acres Planted will remain at 100 acres, but now the value of Acres Treated will be 150. This figure may not be the total acreage of the selected crop. See documentation for further distinctions between acres treated and acres planted ...
Erysipelas and cellulitis are two infections with similar characteristics that develop when bacteria beyond the barrier of the skin, invading and infecting deeper tissues. The term cellulite cause some confusion to designate two different diseases. Those irregularities in the skin…Continue Reading →. ...
Photograph of ethidium bromide stained 3′RACE products electrophoresed on a 2% sieving agarose gel. Numbers on the left show the sizes of molecular marker (M)
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A little piece of heaven...this 6 acre parcel is the smallest of the (4) parcels. There is a nice level walking path through this piece of land. Purchase all 4 parcels APN # 074-030-004-000, 074-030-003-000, 074-020-017-000, 074-030-002-000 for a total of 63.91 Acres for $225,000 Owner financing we
Behandling i almen praksis Anbefalet behandling VoksnePhenoxymethylpenicillin660 mg (1 mill. IE) p.o. x 4 i 7 dage ved tilfredsstillende klinisk effekt. BørnPhenoxymethylpenicillin50 mg (0,08 mill
Soil pH should be at least 6.5 or above. Corn uses large amounts of Nitrogen. The total amount applied depends on yield goals and overall management. For 120 bu/acre yield, about 140 lbs. N/acre should be applied. For 160 bu/acre yield, about 190 lbs N/acre should be applied. Depending on economics of application, it is best to apply N in split applications, with a small amount at planting and the remainder sidedressed before the 10 week after planting. Application of P and K should follow soil test recommendations ...
Lee K, Zhang H, Qian DZ, Rey S, Liu JO, Semenza GL (October 2009). "Acriflavine inhibits HIF-1 dimerization, tumor growth, and ... so HIF inhibitors such as phenethyl isothiocyanate and Acriflavine are (since 2006) under investigation for anti-cancer effects ...
Commonly used fluorescent stains include topically applied acriflavine, and intravenously administered fluorescein sodium. ...
Topical treatment of acriflavine or berenil applied to prepTransmissionuce has been proven effective for treatment. There are ...
Chan-Curtis, Victoria Lye-Hua (October 1969). Cytochemical localization of the nucleic acids by an acriflavine-phosphotungstate ...
Petite mutants can be generated in the laboratory by using high-efficiency treatments such as acriflavine, ethidium bromide, ...
... , also called proflavin and diaminoacridine and bate na bina, is an acriflavine derivative, a disinfectant ...
He also worked on anti-malarial drugs and proposed Acriflavine as an anti-malarial agent, which has also been patented by him. ... Entamoeba histolytica Amoebiasis ORC6 Geminin Helicobacter pylori Plasmodium falciparum Acriflavine Tapas Kumar Kundu India ...
... acriflavine MeSH D03.494.046.250.200 --- aminacrine MeSH D03.494.046.250.225 --- amsacrine MeSH D03.494.046.250.450 --- ...
... was successful in identifying acriflavine, an anti-parasitic drug in use in the 20th century, to be effective against ...
... acriflavine hydrochloride. He found that in the teeth of rats fed a cariogenic diet the flow of dentinal fluid was markedly ...
He played a crucial role in the development of several important molecules such as acriflavine, bestatine, ellipticine, ...
3 tablespoons of salt for every one gallon of water Acriflavine: a powerful dye used for treating protozoans, fungus, ...
... Encyclopædia Britannica Acriflavine use in aquaria Lee, K.; Zhang, H.; Qian, D. Z.; Rey, S.; Liu, J. O.; Semenza, G ... Acriflavine (INN: acriflavinium chloride) is a topical antiseptic. It has the form of an orange or brown powder. It may be ... Acriflavine was developed in 1912 by Paul Ehrlich, a German medical researcher, and was used during the First World War against ... Acriflavine is used in biochemistry for fluorescently labeling high molecular weight RNA. It is used as treatment for external ...
The Escherichia coli Acriflavine resistance (acrA and acrB genes) encode a multi-drug efflux system that is believed to protect ...
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acriflavine Encyclopædia Britannica Acriflavine use in aquaria Lee, K.; Zhang, H.; Qian, D. Z.; Rey, S.; Liu, J. O.; Semenza, G ... Acriflavine (INN: acriflavinium chloride) is a topical antiseptic. It has the form of an orange or brown powder. It may be ... Acriflavine was developed in 1912 by Paul Ehrlich, a German medical researcher, and was used during the First World War against ... Acriflavine is used in biochemistry for fluorescently labeling high molecular weight RNA. It is used as treatment for external ...
The Escherichia coli Acriflavine resistance (acrA and acrB genes) encode a multi-drug efflux system that is believed to protect ...
What is acriflavine hydrochloride? Meaning of acriflavine hydrochloride medical term. What does acriflavine hydrochloride mean? ... Looking for online definition of acriflavine hydrochloride in the Medical Dictionary? acriflavine hydrochloride explanation ... redirected from acriflavine hydrochloride). Also found in: Dictionary, Encyclopedia. fla·vin. , flavine (flāvin, -vēn, flavin ... 2. Any of several acridine derivatives, such as acriflavine, formerly used as antiseptics. ...
Acriflavines MSDS Sheet Acriflavine MSDS - by MSDS Dept. Acriflavine MSDS. Section I - IDENTIFICATION. PRODUCT: Acriflavine ... Acriflavine for Koi Diseases Acriflavine - by Doc Johnson. General Information. Acriflavine is efffective against external ... Acriflavine might be the silver bullet for Costia but it stains you badly, and kills plants.. ... Doc Johnson: More work is needed on Acriflavine. We need to establish the effect on filter bacteria, most of the common pond ...
It is the SmvA efflux pump, and not AcrB, that plays the major role in the efflux of acriflavine and other QACs from Salmonella ... Additionally, the smvA mutant strain was more susceptible to acriflavine than the acrB mutant (MICs = 31.3 versus 125 mg/L, i.e ... SmvA, and not AcrB, is the major efflux pump for acriflavine and related compounds in Salmonella enterica serovar Typhimurium. ... A tolC single mutant was more susceptible than an acrB mutant to QACs, acriflavine, ethidium bromide, malachite green and ...
Baird-Parker medium supplemented with acriflavine, polymyxins and sulphonamide for the selective isolation of Staphylococcus ...
The effects of acriflavine on two species of Trypanosomatidae, Crithidia luciliae and Trypanosoma mega, have been investigated ... THE LOSS OF KINETOPLASTIC DNA IN TWO SPECIES OF TRYPANOSOMATIDAE TREATED WITH ACRIFLAVINE. M. Steinert, Suzanne van Assel ... Radioautography was used to study the effects of acriflavine on thymidine-3H incorporation in C. luciliae. At the concentration ... It has been observed that kinetoplastic (i.e. mitochondrial) DNA is lost in a high percentage of acriflavine-treated cells. ...
Acriflavine is a topical antiseptic. It has the form of an orange or brown powder. It may be harmful in the eyes or if inhaled ... Acriflavine may also be used as an alternative to Malachite Green when it is known that certain species of fish may be ... Acriflavine is a preventative and treatment for the control of Oodinium (Velvet) in freshwater and marine fishes. It is also ... Acriflavine is useful for fish egg disinfection, treatment of open wounds, and external protozoan infections. The chemical said ...
Acriflavine is a fluorescent dye for labeling high molecular weight RNA. It is also a topical antiseptic. ... Acriflavine is a fluorescent dye for labeling high molecular weight RNA. It is also a topical antiseptic. ...
ACRIFLAVINE HYDROCHLORIDE \ 646 for more molecular products just contact us ... We have also other products like : ACRIFLAVINE HYDROCHLORIDE Related products : ACRIFLAVINE HYDROCHLORIDE ... 30236645] Optimized acriflavine-loaded lipid nanocapsules as a safe and effective delivery system to treat breast cancer.. [ ... 24309178] Investigation on the inclusion and toxicity of acriflavine with cyclodextrins: a spectroscopic approach.. [24237726] ...
ISAba1 movement was observed in the acriflavine mutants Ab153M and Ab1225M. Ab153M exhibited an increase in the MIC values of ... This study evaluated the role of acriflavine, a frameshift mutagen, on the movement of insertion sequence ISAba1 in clinical ... Effect of frameshift mutagen acriflavine on control of resistance genes in Acinetobacter baumannii * B. S. Lopes1, A. Hamouda1 ... ISAba1 movement was observed in the acriflavine mutants Ab153M and Ab1225M. Ab153M exhibited an increase in the MIC values of ...
Acriflavine is a fluorescent dye for labeling high molecular weight RNA. It is also a topical antiseptic. - Mechanism of Action ... Acriflavine is identified as a potent inhibitor of the MCT4 that can inhibit the binding between Basigin and MCT4. Acriflavine ... Acriflavine treatment inhibits intratumoral expression of VEGF and tumor vascularization[1]. In a murine CML model, acriflavine ... Acriflavine retards tumor growth in a murine model of breast cancer. The combination of sunitinib with acriflavine ...
HomeUncategorizedIntravital accumulation of acriflavine in m. Intravital accumulation of acriflavine in m. By admin April 25, ...
... trader of Acriflavine Neutral BPC-49 (Euflavine) at the best price. ... Acriflavine Neutral BPC-49 (Euflavine). We , SHREEJI PHARMA International, established in 2004, are proud to introduce ...
However, this is the first study to show that catalase is required for the toxicity of acriflavine. Our results imply that ... Loss of acrA also confers cross-resistance to unrelated compounds such as acriflavine and thiabendazole. We have used ... Disruption of the catA gene results in parallel resistance to acriflavine. Molecular and biochemical studies of several ... acriflavine and thiabendazole are precursors which must be oxidized to generate biologically active species. The catA/acrA gene ...
ACRIFLAVINE. ChEMBL Synonyms ACRIFLAVINE , Acriflavinium Chloride , FLAVAZOLE IN CARBOWAX. Max Phase. 4 (Approved). ...
Cloverleaf Acriflavine Answer 1L. £16.99. Out of stock, back soon EMAIL WHEN IN STOCK Out of Stock, Back Soon ...
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Acriflavine neutral (euflavin) bioWORLD. A dye widely used as an antifungal and antiparasitic agent in aqueous culture systems ...
K. Lee, H. Zhang, D. Z. Qian, S. Rey, J. O. Liu, and G. L. Semenza, "Acriflavine inhibits HIF-1 dimerization, tumor growth, and ... acriflavine), decreased DNA binding to the HIF (anthracyclines and echinomycin), and reduced transcriptional activity [1]. (a) ...
Acriflavine (antiseptic and dye) Acriflavine, dye obtained from coal tar, introduced as an antiseptic in 1912 by the German ...
Acriflavine. *Acriflavine is a topical antiseptic.. *Acriflavine is also used as treatment for external fungal infections of ... ACRIFLAVINE. ACTINOMYCIN. ACTIVE INGREDIENT. ADVERSE EVENT. AFRIN. ALAMETHICIN. ALBENDAZOLE. ALFUZOSIN. ALKYLATING AGENT. ... Acriflavine was developed in 1912 by Paul Ehrlich, a German medical researcher and was used during the First World War against ...
A total of 414 coagulase-positive staphylococcal strains obtained at the mastitis laboratory, National Veterinary Institute, Uppsala, Sweden, were studied. One hundred and seventy seven strains were used for a frequency study. Ninety-seven per cent were identified as Staphylococcus aureus, 2% as Sta …
  • A tolC single mutant was more susceptible than an acrB mutant to QACs, acriflavine, ethidium bromide, malachite green and pyronin B. The tolC-acrB double mutant was as susceptible as the single tolC mutant to QACs. (nih.gov)
  • Petite mutants can be generated in the laboratory by using high-efficiency treatments such as acriflavine, ethidium bromide, and other intercalating agents. (wikipedia.org)
  • Baird-Parker medium supplemented with acriflavine, polymyxins and sulphonamide for the selective isolation of Staphylococcus aureus from heavily co. (nih.gov)
  • As early as 1968, Simpson (10) showed that acriflavine in low concentrations, through selective inhibition of replication of kinetoplast DNA and not nuclear DNA is able to induce dyskinetoplasia in cultures of L. tarentolae. (thefreelibrary.com)
  • The selective components acriflavine and nalidixic acid are blended into the base powder and the ferric ammonium citrate (X211 or 7984) is added to the tempered broth after sterilization. (neogen.com)
  • Listeria Enrichment Broth, Modified (reduced acriflavine), selective enrichment of Listeria in dairy products. (spectrumchemical.com)
  • Our results suggest that tolC and smvA genes encode components of two different efflux systems with overlapping specificities that work in parallel to export acriflavine and other QACs. (nih.gov)
  • Comparison of the MICs and minimal bactericidal concentrations of antimicrobials for bpeAB deletion mutant KHWΔ bpeAB and its isogenic wild-type parent, KHW, showed that the B. pseudomallei BpeAB-OprB pump is responsible for the efflux of the aminoglycosides gentamicin and streptomycin, the macrolide erythromycin, and the dye acriflavine. (asm.org)
  • To the best of our knowledge, the toxicological properties of acriflavine neutral have not been thoroughly investigated. (koivet.com)
  • In contrast, fish treated with acriflavine neutral were inferior to fish not treated with any chemical in both appearance and behavior. (ufl.edu)
  • Inhibition of hypoxia-inducible factor (HIF)-α transcription factors with acriflavine as third-line therapy had therapeutic effects in some tumors, providing preclinical evidence for further investigation of HIF-α inhibition as a ccRCC treatment. (nature.com)
  • Expression of NPC1 in Escherichia coli (i) facilitated the transport of acriflavine across the plasma membrane, causing cytosolic accumulation, and (ii) resulted in transport of oleic acid but not cholesterol or cholesterol-oleate across the plasma membrane. (sciencemag.org)
  • The susceptibility of these strains to acriflavine and a variety of QACs was determined by MIC assays. (nih.gov)
  • Of the serotype 1a isolates, 64.6% were acriflavine resistant, with 98.4% of these acriflavine-resistant strains having been isolated from animals with chronic arthritis. (asm.org)
  • By randomly amplified polymorphic DNA (RAPD) analysis, almost all the acriflavine-resistant serotype 1a strains showed the 253-bp band characteristic of vaccine strains and were easily discriminated from all 113 strains of acriflavine-sensitive serotype 1a strains from animals with acute and subacute swine erysipelas. (asm.org)
  • The incidence of acriflavine-resistant strains of the distinctive RAPD type 1-2 was markedly higher than that of the other RAPD types and serotypes. (asm.org)
  • Similar between-slope differences were found for ascospore germination-resistance to acriflavine, with much higher frequencies in strains from the south-facing slope. (genetics.org)
  • Demethylation inhibitor (DMI)-resistant strains of the plant pathogenic fungus Penicillium digitatum were shown to be simultaneously resistant to cycloheximide, 4-nitroquinoline- N -oxide (4NQO), and acriflavine. (asm.org)
  • Acriflavine may also be used as an alternative to Malachite Green when it is known that certain species of fish may be sensitive (small Characins ). (theaquariumwiki.com)
  • Acriflavine might be the silver bullet for Costia but it stains you badly, and kills plants. (koivet.com)
  • All serotype 1a isolates were further examined for acriflavine resistance and their genotypes to discriminate them from the attenuated live vaccine strain, defined as serotype 1a, which is resistant to 0.02% acriflavine and which shows low levels of pathogenicity in mice. (asm.org)
  • Acriflavine might be effective in fighting common cold virus, and also aid the fight against increasingly antibiotic resistant bacteria. (wikipedia.org)
  • This study evaluated the role of acriflavine, a frameshift mutagen, on the movement of insertion sequence IS Aba1 in clinical isolates of A. baumannii , with the focus on changes in expression levels of the bla ADC and bla OXA-51-like genes. (microbiologyresearch.org)
  • Mice: CML mice are treated daily with acriflavine (8 mg/kg) or PBS via intraperitoneal injection, for 10 days starting from day 7 after bone marrow transplantation . (medchemexpress.com)
  • Physical chemical studies on the specific interaction of an acriflavine-phosphotungstic Acid complex with double-stranded nucleic acids. (harvard.edu)
  • In Japan, unlike in other countries, only live vaccine has been used for a long time because the acriflavine-resistant attenuated live vaccine was first developed in Japan in 1932 ( 8 ). (asm.org)
  • Since 2014, acriflavine has been undergoing testing as an antimalarial drug to treat parasites with resistance to quinine and modern anti-parasitic medicines. (wikipedia.org)
  • Acriflavine is a controlled substance in Australia and dependent on situation,[clarification needed] is considered either a Schedule 5 (Caution) or Schedule 7 (Dangerous Poison) substance. (wikipedia.org)
  • It has been observed that kinetoplastic (i.e. mitochondrial) DNA is lost in a high percentage of acriflavine-treated cells. (rupress.org)
  • During growth in acriflavine cultures, cells were seen to exhibit an increasing impairment of colony-forming ability and rate of respiration. (thefreelibrary.com)
  • However, this is the first study to show that catalase is required for the toxicity of acriflavine. (microbiologyresearch.org)
  • In this study in, in order to design a new chemotherapy protocol, the effect of 5-FU and acriflavine (ACF) cotreatment on mortality rate of CRC cell lines was investigated. (ac.ir)
  • Our results imply that acriflavine and thiabendazole are precursors which must be oxidized to generate biologically active species. (microbiologyresearch.org)
  • The combination of sunitinib with acriflavine significantly decreases vascular endothelial growth factor and TGF-β expression and reduces tumor vasculature followed by increased intratumor necrosis and apoptosis . (medchemexpress.com)
  • Studies of acriflavine loading in normal and NPC1 fibroblasts indicated that NPC1 uses a proton motive force to remove accumulated acriflavine from the endosomal/lysosomal system. (sciencemag.org)