Acridine Orange
Citrus sinensis
Aminoacridines
Staining and Labeling
Fluorescent Dyes
Ethidium
A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide.
Intercalating Agents
Azure Stains
Microscopy, Fluorescence
Gentian Violet
Citrus
Coloring Agents
Cardiolipins
Acidic phospholipids composed of two molecules of phosphatidic acid covalently linked to a molecule of glycerol. They occur primarily in mitochondrial inner membranes and in bacterial plasma membranes. They are the main antigenic components of the Wassermann-type antigen that is used in nontreponemal SYPHILIS SERODIAGNOSIS.
Lysosomes
A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Benzophenoneidum
Hydrogen-Ion Concentration
Aminacrine
Histiocytoma
Comparison of five methods of malaria detection in the outpatient setting. (1/413)
In eastern Africa where 90% of the malaria is due to Plasmodium falciparum, the accuracy of malaria diagnosis at the outpatient level is becoming increasingly important due to problems of drug resistance and use of alternative, costly antimalarial drugs. The quantitative buffy coat (QBC) technique, acridine orange staining with an interference filter system, and the ParaSight-F test have been introduced as alternative methods to conventional microscopy for the diagnosis of malaria. Two hundred thirteen outpatients were tested using these alternative methods and conventional microscopy by five experienced technologists; two were randomly allocated to read the results of each test. Paired results showed the highest level of agreement with the ParaSight-F test (99%), followed by Field stain (92%). The results of the QBC technique showed the least agreement (73%). Using conventional microscopy as the reference standard, the ParaSight-F test had a sensitivity range of 90-92% and a specificity of 99%, staining with acridine orange had a sensitivity range of 77-96% and a specificity range of 81-98% and the QBC technique had a sensitivity range of 88-98% and a specificity range of 58-90%. All microscopic tests showed lower sensitivities (as low as 20% using staining with acridine orange) in detecting low parasitemias (< or = 320/microl) than the ParaSight-F test (70%). Due to the high cost of the ParaSight-F test, Field-stained blood films remain the most appropriate method for diagnosis of P. falciparum in eastern Africa. The ParaSight-F test may be used in situations where no trained microscopists are available, or where malaria is strongly suspected and the results of microscopy are negative. (+info)Destruction of protamine in human sperm inhibits sperm binding and penetration in the zona-free hamster penetration test but increases sperm head decondensation and male pronuclear formation in the hamster-ICSI assay. (2/413)
PURPOSE: Our purpose was to investigate the fertilizing ability of human protamine-damaged sperm in a heterologous system using hamster oocytes. METHODS: The protamine of the sperm were damaged by exposure to dithiothreitol, a disulfide-reducing agent. Their ability to penetrate and form male pronuclei were investigated using the zona-free hamster penetration test and the hamster-intracytoplasmic sperm injection assay, respectively. RESULTS: The zona-free hamster penetration test revealed that protamine-damaged sperm are unable to bind and penetrate the hamster oocyte. On the other hand, hamster-intracytoplasmic sperm injection assay results showed that 56.9% and 39.2% of the injected oocytes developed male pronuclei in protamine-damaged and live-intact sperm groups, respectively, with a significant difference in these rates (P < 0.01). CONCLUSIONS: This study shows that protamine-damaged sperm are able to undergo sperm head decondensation and male pronuclear formation only when injected into the ooplasm, although they cannot bind and penetrate through the zona and enter the ooplasm. (+info)Reactive oxygen species-induced apoptosis and necrosis in bovine corneal endothelial cells. (3/413)
PURPOSE: The loss of corneal endothelial cells associated with aging and possibly other causes has been speculated to be related to exposure to reactive oxygen species (ROS). The current study was conducted to investigate, by use of photosensitizers, the underlying mechanisms involved in the death of bovine corneal endothelial cells (BCENs) caused by ROS. METHODS: BCEN cells in primary culture were treated with a photosensitizer (riboflavin or rose bengal) with light exposure. The patterns of cell damage and death were assessed using an acridine orange-ethidium bromide differential staining method, TdT-mediated dUTP nick-end labeling (TUNEL) assay, and transmission electron microscopy. The cytotoxicity was assayed by mitochondrial function using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) testing. Antioxidants, including catalase, L-histidine, salicylic acid, and superoxide dismutase, were used to determine the types of ROS involved. Activation of nuclear factor (NF)-kappaB was examined by fluorescent immunocytochemistry with anti-p65 antibody. RESULTS: Light-irradiated riboflavin or rose bengal resulted in a significant decrease in viability of BCEN cells. Chromosomal condensation and fragmentation were observed in apoptotic cells, and membrane lysis and damage of cell ultrastructures were observed in necrotic cells. Riboflavin induced apoptosis at 30 minutes and thereafter and induced necrosis after 2 hours. Rose bengal was shown to cause similar effects within half the time required for the effects of riboflavin. Catalase and salicylic acid were found to provide protection for BCENs from cytotoxic effects of riboflavin, and L-histidine was found to protect BCENs from cytotoxicity induced by rose bengal. Kinetic studies using immunocytochemistry showed that NF-kappaB was translocated into the nucleus within 15 minutes and 30 minutes after treatment with rose bengal and riboflavin, respectively. CONCLUSIONS: The cytotoxic effects of photo-irradiated riboflavin and rose bengal are shown to be mediated by two distinct but parallel pathways, one leading to apoptosis and the other to necrosis. Possible involvement of NF-kappaB in cell death is suggested. These findings provide potential leads for future investigation into the molecular mechanisms of loss of corneal endothelial cells related to aging, oxidative stress, and possibly other similar causes. (+info)Differential chemosensitivity of breast cancer cells to ganciclovir treatment following adenovirus-mediated herpes simplex virus thymidine kinase gene transfer. (4/413)
The development of resistance to radiation and chemotherapeutic agents that cause DNA damage is a major problem for the treatment of breast and other cancers. The p53 tumor suppressor gene plays a direct role in the signaling of cell cycle arrest and apoptosis in response to DNA damage, and p53 gene mutations have been correlated with increased resistance to DNA-damaging agents. Herpes simplex virus thymidine kinase (HSV-tk) gene transfer followed by ganciclovir (GCV) treatment is a novel tumor ablation strategy that has shown good success in a variety of experimental tumor models. However, GCV cytotoxicity is believed to be mediated by DNA damage-induced apoptosis, and the relationship between p53 gene status, p53-mediated apoptosis, and the sensitivity of human tumors to HSV-tk/GCV treatment has not been firmly established. To address this issue, we compared the therapeutic efficacy of adenovirus-mediated HSV-tk gene transfer and GCV treatment in two human breast cancer cell lines: MCF-7 cells, which express wild-type p53, and MDA-MB-468 cells, which express high levels of a mutant p53 (273 Arg-His). Treating MCF-7 cells with AdHSV-tk/GCV led to the predicted increase in endogenous p53 and p21WAF1/CIP1 protein levels, and apoptosis was observed in a significant proportion of the target cell population. However, treating MDA-MB-468 cells under the same conditions resulted in a much stronger apoptotic response in the absence of induction in p21WAF1/CIP1 protein levels. This latter result suggested that HSV-tk/GCV treatment can activate a strong p53-independent apoptotic response in tumor cells that lack functional p53. To confirm this observation, four additional human breast cancer cell lines expressing mutant p53 were examined. Although a significant degree of variability in GCV chemosensitivity was observed in these cell lines, all displayed a greater reduction in cell viability than MCF-7 or normal mammary cells treated under the same conditions. These results suggest that endogenous p53 status does not correlate with chemosensitivity to HSV-tk/GCV treatment. Furthermore, evidence for a p53-independent apoptotic response serves to extend the potential of this therapeutic strategy to tumors that express mutant p53 and that may have developed resistance to conventional genotoxic agents. (+info)Evanescent-wave microscopy: a new tool to gain insight into the control of transmitter release. (5/413)
Evanescent-wave excitation was used to visualize individual fluorescently labelled vesicles in an optical slice near the plasma membrane of bovine adrenal chromaffin cells. A standard upright microscope was modified to accommodate the optics used for directing a laser beam under a supracritical angle on to the glass-water interface on top of which the cells are grown. Whereas epi-illumination images appeared blurred and structureless, evanescent-wave excitation highlighted acridine orange-labelled vesicles as individual pinpoints. Three-dimensional (3D) trajectories of individual vesicles were obtained from time-resolved image stacks and used to characterize vesicles in terms of their average fluorescence F and mobility, expressed here as the 3D diffusion coefficient D(3). Based on the single-vesicle analysis, two groups of vesicles were identified. Transitions between these states were studied before and after stimulation of exocytosis by repetitive or maintained membrane depolarizations by elevated extracellular [K+]. Findings were interpreted as sequential transitions between the previously characterized pools of vesicles preceding the fusion step. The observed approach of vesicles to their docking sites was not explained in terms of free diffusion: most vesicles moved unidirectionally as if directed to their binding sites at the plasma membrane. Vesicle mobility at the membrane was low, such that the sites of docking and fusion were in close vicinity. Both the rim region and confined areas in the centre of the footprint region were the site of intense vesicle trafficking. (+info)Arrest of endosome acidification by bafilomycin A1 mimics insulin action on GLUT4 translocation in 3T3-L1 adipocytes. (6/413)
In insulin-sensitive fat and muscle cells, the major glucose transporter GLUT4 is constitutively sequestered in endosomal tubulovesicular membranes, and moves to the cell surface in response to insulin. While sequence information within GLUT4 appears to be responsible for its constitutive intracellular sequestration, the regulatory elements and mechanisms that enable this protein to achieve its unique sorting pattern under basal and insulin-stimulated conditions are poorly understood. We show here that arrest of endosome acidification in insulin-sensitive 3T3-L1 adipocytes by bafilomycin A1, a specific inhibitor of the vacuolar proton pump, results in the rapid and dose-dependent translocation of GLUT4 from the cell interior to the membrane surface; the effects of maximally stimulatory concentrations of bafilomycin A1 (400-800 nM) were equivalent to 50-65% of the effects of acute insulin treatment. Like insulin, bafilomycin A1 induced the redistribution of GLUT1 and Rab4, but not that of other proteins whose membrane localization has been shown to be insulin-insensitive. Studies to address the mechanism of this effect demonstrated that neither autophosphorylation nor internalization of the insulin receptor was altered by bafilomycin A1 treatment. Bafilomycin-induced GLUT4 translocation was not blocked by cell pretreatment with wortmannin. Taken together, these data indicate that arrest of endosome acidification mimics insulin action on GLUT4 and GLUT1 translocation by a mechanism distal to insulin receptor and phosphatidylinositol 3-kinase activation, and suggest an important role for endosomal pH in the membrane dynamics of the glucose transporters. (+info)Radiation-induced leukocyte entrapment in the rat retinal microcirculation. (7/413)
PURPOSE: To evaluate the effects of irradiation on leukocyte dynamics in the rat retinal microcirculation. METHODS: Thirty-five Brown-Norway rats received a dose of 10 Gy irradiation in one fraction. Leukocyte dynamics were studied with acridine orange digital fluorography, in which a nuclear fluorescent dye of acridine orange is used and examined by scanning laser ophthalmoscope. This technique allowed visualization of fluorescent leukocytes in vivo. The leukocyte dynamics were evaluated at 0, 4, 7, 14, 30, and 60 days after the irradiation. RESULTS: Mean leukocyte velocity in the retinal capillaries decreased immediately. It partially recovered on day 4 but then gradually decreased up to the 2-month mark. Low-dose irradiation induced entrapment of leukocytes in the retinal microcirculation. The number of entrapped leukocytes gradually increased with time. The major retinal vessels significantly constricted immediately after irradiation. The diameter was reduced by 76% in arteries and 75% in veins, 2 months after irradiation. CONCLUSIONS: Entrapped leukocytes may be activated and exacerbate vascular injury and microinfarction and thus may participate in the pathogenesis of radiation retinopathy. (+info)Tick-borne relapsing fever imported from West Africa: diagnosis by quantitative buffy coat analysis and in vitro culture of Borrelia crocidurae. (8/413)
West African tick-borne relapsing fever (TBRF) is difficult to diagnose due to the low number of spirochetes in the bloodstream of patients. Previously, the causative microorganism, Borrelia crocidurae, had never been cultured in vitro. TBRF was rapidly diagnosed for two patients returning from western Africa with fever of unknown origin by quantitative buffy coat (QBC) analysis. Diagnosis was confirmed by intraperitoneal inoculation of blood specimens from patients into laboratory mice. In vitro experiments showed that QBC analysis may be as much as 100-fold more sensitive than thick smear. Spirochetes were also cultured from blood samples from both patients in modified Kelly's medium and were identified as B. crocidurae by partial sequencing of the PCR-amplified rrs gene. (+info)Mitochondrial Membrane Potential Assay Kit (Orange Fluorescence) Kit-0560 - Creative BioMart
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Acridine Orange
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Metachromasy in clay dye systems; the adsorption of acridine orange by Na-beidellite | Clay Minerals | GeoScienceWorld
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Acridine Orange
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Localization and identification of populations of phosphatase-active bacterial cells associated with activated sludge flocs
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Prevalence of multiple antibiotic resistance in 443 Campylobacter spp. isolated from humans and animals : Journal of...
Acridine Forum
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Spermatozoa bound to solid state hyaluronic acid show chromatin structure with high DNA chain integrity: an acridine orange...
The reliability of diagnostic techniques in the diagnosis and management of malaria in the absence of a gold standard. -...
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Acridine | definition of acridine by Medical dictionary
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Chlorophyllin
... also binds to acridine orange. Chlorophyllin has been validated to exhibit ameliorative effects against food ...
Fluorophore
Acridine derivatives: proflavin, acridine orange, acridine yellow, etc. Arylmethine derivatives: auramine, crystal violet, ... DRAQ7 and CyTRAK Orange Pyrene derivatives: cascade blue, etc. Oxazine derivatives: Nile red, Nile blue, cresyl violet, oxazine ...
Metachromasia
Darzynkiewicz Z, Kapuscinski J. (1990)"Acridine Orange, a Versatile Probe of Nucleic Acids and Other Cell Constituents." ... Another example of metachromatic dye (fluorochrome) is acridine orange. Under certain conditions it stains single-stranded ...
Vital stain
Hathaway WE, Newby LA, Githens JH (1964). "The Acridine Orange Viability Test Applied to Bone Marrow Cells. I. Correlation with ...
Oleocanthal
Cell apoptosis is tested by treating the lysosomal membrane with acridine orange. Acridine orange radiates a red fluorescent ...
Lysosome
Traganos F, Darzynkiewicz Z (1994). "Lysosomal proton pump activity: supravital cell staining with acridine orange ...
GelGreen
... consists of two acridine orange subunits that are bridged by a linear oxygenated spacer. Its fluorophore, and ... Ethidium bromide GelRed SYBR Green I Agarose gel electrophoresis and gel electrophoresis of nucleic acids Acridine orange US ... therefore its optical properties, are essentially identical to those of other N-alkylacridinium orange dyes. When exposed to ...
Staining
Acridine orange (AO) is a nucleic acid selective fluorescent cationic dye useful for cell cycle determination. It is cell- ... The stain may also be used in conjunction with acridine orange (AO) in viable cell counting. This EB/AO combined stain causes ... In a skillfully made H&E preparation the red blood cells are almost orange, and collagen and cytoplasm (especially muscle) ... It uses a combination of haematoxylin, Orange G, eosin Y, Light Green SF yellowish, and sometimes Bismarck Brown Y. Periodic ...
Sperm Chromatin Structure Assay
Acridine orange (AO) staining: Next, 1.20 ml of AO staining solution with 6 µg AO/ml staining buffer is added into the mixture ... Through acridine orange (AO) staining, AO molecules are intercalated into double-stranded DNA in intact sperms while ... Acridine orange test and toluidine blue assay". Andrologia. 49 (10): e12765. doi:10.1111/and.12765. PMID 28224638. S2CID ...
Microbial loop
1977). Bacterial cells were counted with an epifluorescence microscope, producing what is called an "acridine orange direct ...
Microscopy with UV surface excitation
Several dyes have been studied for MUSE's application, including eosin, rhodamine, DAPI, Hoechst, acridine orange, propidium ...
Buffy coat
The blood is taken in a QBC capillary tube which is coated with acridine orange (a fluorescent dye) and centrifuged; the ...
Diagnosis of malaria
The blood is taken in a QBC capillary tube which is coated with acridine orange (a fluorescent dye) and centrifuged; the ...
Bacterioplankton counting methods
Fluorescent dyes include DAPI, Acridine Orange, SYBR Green 1, and YO-PRO-1, all of which are capable of staining both DNA and ... Gonzalez K, Mcvey S, Cunnick J, Udovichenko IP, Takemoto DJ (1995). "Acridine orange differential staining of total DNA and RNA ... while Synechococcus emits both orange and red fluorescence; orange from phycobilins and red from chlorophyll. Besides ...
Wilhelm Balthasar
Cooperative Complex Formation of Acridine Orange with poly-l-glutamic Acid] (in German). University of California. OCLC ...
Cardiolipin
M Garciafernandez; D Ceccarelli; U Muscatello (2004). "Use of the fluorescent dye 10-N-nonyl acridine orange in quantitative ... Jacobson J, Duchen MR, Heales SJ (2002). "Intracellular distribution of the fluorescent dye nonyl acridine orange responds to ... Keij JF, Bell-Prince C, Steinkamp JA (2000). "Staining of mitochondrial membranes with 10-nonyl acridine orange, MitoFluor ... Based on this special structure, the fluorescent mitochondrial indicator, nonyl acridine orange (NAO) was introduced in 1982, ...
CD47
Affected cells do not exhibit hallmarks of apoptosis but rather autophagy as seen by staining with acridine orange and ...
Borrelia turicatae
B. turicatae is best seen by dark-field microscopy, but the organisms can also be detected using acridine orange or Wright's ...
OLED
They applied high alternating voltages in air to materials such as acridine orange dye, either deposited on or dissolved in ...
Apicomplexan life cycle
... as revealed by acridine orange staining and PCR-based diagnoses". Tropical Medicine and International Health. 3 (4): 304-312. ...
Helicobacter pylori
... acridine orange stain, and phase-contrast microscopy. It is capable of forming biofilms and can convert from spiral to a ...
Cell cycle analysis
An exception is the metachromatic fluorochrome acridine orange, which under the specific staining protocol can differentially ... or DNA susceptibility to denaturation at low pH using the metachromatic dye acridine orange, reveals the G1Q, G1A, and G1B cell ... Aside from propidium iodide and acridine orange, quantifiable dyes that are frequently used include (but are not limited to) ...
Conductive polymer
... when Bernanose and coworkers first produced electroluminescence in crystalline thin films of acridine orange and quinacrine. In ...
Sonodynamic therapy
... include acridine orange, methylene blue, curcumin, and indocyanine green. A study by Suzuki et al. used acridine orange, a ...
List of MeSH codes (D03)
... acridine orange MeSH D03.494.046.250.177 - acriflavine MeSH D03.494.046.250.200 - aminacrine MeSH D03.494.046.250.225 - ...
Photodermatitis
Psoralens, coal tars, photo-active dyes (eosin, acridine orange) Musk ambrette, methylcoumarin, lemon oil (may be present in ... In these areas an unsightly orange to brown tint may form, usually near or on the face. Many medications and conditions can ...
Apoptotic DNA fragmentation
The flow-cytometric assay utilizing the fluorochrome acridine orange shows that DNA fragmentation within individual cells is ...
Piscirickettsia salmonis
Smears of the kidney, liver, and spleen can be stained with Gram, Giemsa, acridine orange, or methylene blue stain for direct ...
C17H19N3
The molecular formula C17H19N3 (molar mass : 265.35 g/mol) may refer to: Acridine orange Antazoline Esmirtazapine Mirtazapine A ...
Escherichia coli in molecular biology
... and then in 1985 of the F plasmid by acridine orange curing.[citation needed] Strains derived from MG1655 include DH1, parent ...
Acriflavine
It has the form of an orange or brown powder. It may be harmful in the eyes or if inhaled. It is a dye and it stains the skin ... It is derived from acridine. Commercial preparations are often mixtures with proflavine. It is known by a variety of commercial ...
Acridine yellow
... damages DNA and is used as a mutagen in microbiology. Acridine yellow is similar to acridine orange. According ... Acridine yellow, also known as acridine yellow G, acridine yellow H107, basic yellow K, and 3,6-diamino-2,7-dimethylacridine, ... It is a derivate of acridine. In histology, it is used as a fluorescent stain, and as a fluorescent probe for non-invasive ... Acridine yellow absorption and emission spectra v t e (Articles needing additional references from July 2021, All articles ...
I-motif DNA
Acridine derivatives are also G4 ligands and through fluorescence resonance energy transfer (FRET) melting assays, ... The ligands for i-motif used as fluorescent probes include Thiazole orange, 2,2'-diethyl-9-methylselenacarbocyanine bromide ( ... Tilorone and Tobramycin are i-motif binding ligands discovered via thiazole orange fluorescence intensity displacement (FID) ... diethylenetriamine (BisA) was determined to increase the melting temperature of both i-motif and G4, while monomeric acridine ( ...
Acridine
... and related derivatives (such as amsacrine) bind to DNA and RNA due to their abilities to intercalate. Acridine orange ... Acridine is easily oxidized by peroxymonosulfuric acid to the acridine amine oxide. The carbon 9-position of acridine is ... at one time acridine dyes were popular, but they are now relegated to niche applications, such as with acridine orange. The ... biologically active acridines, applications of acridines, new syntheses and reactions of acridines] Wikisource has original ...
2,4-Diaminotoluene
Its reaction with benzenediazonium chloride gives the cationic azo dye Basic Orange 1. Condensation of 2,4-diaminotoluene with ... acetaldehyde gives the acridine dye called Basic Yellow 9. NIOSH Pocket Guide to Chemical Hazards. "#0620". National Institute ...
Ethidium bromide
When exposed to ultraviolet light, it will fluoresce with an orange colour, intensifying almost 20-fold after binding to DNA. ... Its core heterocyclic moiety is generically known as a phenanthridine, an isomer of which is the fluorescent dye acridine. ... As a result of this excitation, EtBr emits orange light with wavelength 605 nm. Ethidium bromide's intense fluorescence after ...
Acridine orange
... and fluorescein have a maximum excitation at 502nm and 525 nm (green). When acridine orange associates with RNA ... Acridine orange is useful in the rapid screening of ordinarily sterile specimens. When acridine orange is used with flow ... When the pH of the environment is 3.5, acridine orange becomes excited by blue light (460 nm). When acridine orange is excited ... using acridine orange is a method known for examining the microbial content within food and water. The use of acridine orange ...
IARC group 2B
... acridine Dibenz[c,h]acridine 7H-Dibenzo[c,g]carbazole Dibenzo[a,e]pyrene Dibenzo[a,h]pyrene Dibenzo[a,i]pyrene Dibromoacetic ... Nitrosomethylvinylamine N-Nitrosomorpholine N-Nitrosopiperidine N-Nitrosopyrrolidine N-Nitrososarcosine Ochratoxin A Oil Orange ...
Polymorphism (materials science)
Acridine has been obtained as eight polymorphs and aripiprazole has nine. The record for the largest number of well- ... Krämer, Katrina (2020-07-29). "Red-orange-yellow reclaims polymorph record with help from molecular cousin". chemistryworld.com ... Acridine Solid Form Landscape: Eight Polymorphs and a Hydrate" (PDF). Crystal Growth & Design. 19 (8): 4884-4893. doi:10.1021/ ...
Browsing by Subject "Acridine Orange"
Acridine orange - Wikipedia
Acridine orange and fluorescein have a maximum excitation at 502nm and 525 nm (green). When acridine orange associates with RNA ... Acridine orange is useful in the rapid screening of ordinarily sterile specimens. When acridine orange is used with flow ... When the pH of the environment is 3.5, acridine orange becomes excited by blue light (460 nm). When acridine orange is excited ... using acridine orange is a method known for examining the microbial content within food and water. The use of acridine orange ...
IMSEAR at SEARO: Acridine orange stained blood wet mounts for fluorescent detection of malaria.
Acridine orange stained blood wet mounts for fluorescent detection of malaria.. Authors: Hemvani, N. Chitnis, D S. Dixit, D S. ... The acridine orange stained blood wet mounts over 2420 suspected malaria cases from Indore city were examined under fluorescent ... The modified acridine orange stained method is simple, instant and more efficient, requires less scanning time and skill, ... Acridine orange stained blood wet mounts for fluorescent detection of malaria. Indian Journal of Pathology & Microbiology. 1999 ...
Ex Vivo Confocal Microscopy: Revolution in Fast Pathology
Acetic acid + acridine orange. M-CM. Basal cell carcinoma23 Acridine orange + ethidium bromide. 3-colour FCM. Basal cell ... Acridine orange, methylene blue, toluidine blue, fluorescein, Nile blue or Patent Blue V. FCM. Skin tumours22 ... AO, acridine orange; BCC, basal cell carcinoma; FCM, fluorescence confocal microscopy; NPV, negative predictive value; PPV, ...
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Use of the fluorescent dye 10-N-nonyl acridine orange in quantitative and location assays of cardiolipin: a study on different...
Use of the fluorescent dye 10-N-nonyl acridine orange in quantitative and location assays of cardiolipin: a study on different ... Use of the fluorescent dye 10-N-nonyl acridine orange in quantitative and location assays of cardiolipin: a study on different ... Use of the fluorescent dye 10-N-nonyl acridine orange in quantitative and location assays of cardiolipin: a study on different ... The fluorescent dye 10-N-nonyl acridine orange (NAO) is extensively used for location and quantitative assays of cardiolipin in ...
Sentinel-based Surveillance of Coyotes to Detect Bovine Tuberculosis, Michigan - Volume 14, Number 12-December 2008 - Emerging...
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Changes in the Level of DNA Fragmentation in Sperm Cells detected by Acridine Orange Test in Men with Sub/infertility Treated...
The differences in levels of DNA fragmentation were determined with acridine orange test, which was conducted before and after ... study the effect of PAPA nutritional supplement on the levels of DNA fragmentation of sperm cells tested with acridine orange ... orange and red). After treatment the level of sperm DNA fragmentation decreased by 10.2%. Conclusion: Men with confirmed ...
AO: Acridine orange, PI: propidium iodide, EA: early apoptotic cells, LA: past due apoptotic cells, N: necrotic cells, H:...
Optimization of acridine orange loading on 1:1 layered clay minerals for fluorescence enhancement<...
Optimization of acridine orange loading on 1:1 layered clay minerals for fluorescence enhancement. / Jiang, Wei Teh; Tsai, ... Optimization of acridine orange loading on 1:1 layered clay minerals for fluorescence enhancement. 於: Journal of Industrial and ... 深入研究「Optimization of acridine orange loading on 1:1 layered clay minerals for fluorescence enhancement」主題。共同形成了獨特的指紋。 ... Jiang, W. T., Tsai, Y., Wang, X., & Li, Z. (2020). Optimization of acridine orange loading on 1:1 layered clay minerals for ...
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MitoTracker Orange/MeOH, Cy3™, Rhod-2, Alexa Fluor 555™, tdTomato, DyLight 549, Atto 550, TAMRA, Tetramethylrhodamine ... Acridine Orange + DNA, Atto 488, BCECF/pH 9.0, LysoTracker Green/pH 5.2, Fluo-3, BODIPY FL/pH7.2, Propidium Iodide, Quasar® 570 ... 39009 - AT - Acridine Orange/Di-8-ANEPPS. 39010 - AT - Texas Red/mCherry/AlexaFluor 594. ...
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2011: Mechanism of acridine orange removal from water by low-charge swelling clays, Chem. Engineer. J. (603-611 pp.) ... 2022: Sorption of acridine orange on non-swelling and swelling clay minerals, Crystals (118 pp.) ... 2016: Structure and fluorescent properties of Ba3Sc(PO4)3: Sm3+red-orange phosphor for n-UV w-LEDs, Chem. Phys. Lett. (212-215 ... 2016: Ionic-liquid-crafted zeolite for the removal of anionic dye methyl orange, J. Taiwan Institute Chem. Engineer. (237-243 ...
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Ethidium bromideFluorescenceStainDyesNucleic acidAssayFragmentationApoptotic cellsFluorescent dyeBindsCationicApoptosisEpifluorescence microscopyBacteriaFluoresceinDetectionSmearsFluorochromeIntercalationParasitesMoleculeStainsSpecimensIncubationLysosomalCellsAkridinMethodTissueClay mineralsWidelyDirect countOrganellesMicroscopeStudyBloodWild-typeQualityRelease
Ethidium bromide4
- Apoptosis was evaluated by acridine orange/ethidium bromide double staining method. (scirp.org)
- The real-time reversal of tumor MDR were evaluated by flow cytometer, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, acridine orange/ethidium bromide staining, and Western blot analysis. (dovepress.com)
- Moreover, the pharmacophore modelling evidenced superimposition between linear furanocoumarins, ethidium bromide and acridine orange, thereby suggesting that these compounds share similar biological effects, supported by their acknowledged DNA intercalating activities. (pathofscience.org)
- Generally in most delicate tumor cells (A549), scorpion venom induced necrosis evidenced by acridine orange/ethidium bromide EC1454 fluorescent down-expression and dyes of apoptosis-related genes. (antiviralbiologic.com)
Fluorescence8
- As a cationic fluorescence dye acridine orange (AO) is commonly used in biology and biochemistry for DNA analyses. (ncku.edu.tw)
- TUNEL assay and acridine orange fluorescence staining were carried out to investigate the molecular mechanismsresponsible for the cell death. (ias.ac.in)
- Under a magnification of 1000X, this photomicrograph of a sputum smear, processed using a fluorescence acid-fast staining method that implements acridine orange fluorescent dye as a counterstain, revealed the presence of a Mycobacterium tuberculosis bacterium. (cdc.gov)
- Acridine orange using both epi-fluorescence microscope and flow cytometry. (slu.se)
- SUMMARY: The staining of viral nucleic acids with acridine orange and their subsequent examination under the fluorescence microscope permits the determination of the type and strandedness. (microbiologyresearch.org)
- In this paper, we report simultaneous measurements of nuclear area and nuclear fluorescence intensity on individual cells stained with the intercalating dye, acridine orange (AO). (illinois.edu)
- The Ca2+-induced H+ release visualized by fluorescence quenching of acridine orange correlates well with the Ca2+ uptake determined isotopically. (uc.pt)
- Fluorescence microscopy of cells stained with acridine orange and propidium iodide verified that medications had little influence RAPT1 on cell viability. (researchreport-digital.com)
Stain8
- When acridine orange is excited by blue light, the fluorescent dye can differentially stain human cells green and prokaryotic cells orange (600 nm), allowing for rapid detection with a fluorescent microscope. (wikipedia.org)
- Past and present studies comparing acridine orange staining with blind subcultures for the detection of positive blood cultures showed that the acridine orange is a simple, inexpensive, rapid staining procedure that appears to be more sensitive than the Gram stain for detecting microorganisms in cerebrospinal fluid and other clinical and non-clinical materials. (wikipedia.org)
- When acridine orange is used with flow cytometry, the differential stain is used to measure DNA denaturation and the cellular content of DNA versus RNA in individual cells, or detect DNA damage in infertile sperm cells. (wikipedia.org)
- Acridine orange staining has to be performed at an acidic pH to obtain the differential staining, which allows bacterial cells to stain orange and tissue components to stain yellow or green. (wikipedia.org)
- Hence, the present study was aimed to improve malarial parasite detection by using acridine orange to stain large blood drops in the form of wet coverglass mounts. (who.int)
- This study showed that acridine orange stain is more efficient in demonstrating H. felis organisms than the Romanowsky stains and that the May-Grunwald-Giemsa staining method is superior to the other Romanowsky stains in demonstrating H. felis organisms. (gla.ac.uk)
- Confocal optical section of a living chlorenchyma cell (stained with the nucleic acid stain acridine orange) of Bienertia cycloptera , which possesses a novel mechanism for C 4 photosynthesis. (botany.org)
- You take a stain, like acridine orange or a fluorescent stain or Giemsa , and a microscope, and you look at them. (singjupost.com)
Dyes4
- Acridine dyes are prepared via the condensation of 1,3-diaminobenzene with suitable benzaldehydes. (wikipedia.org)
- Solution 18 contains two different dyes: acridine orange, which stains the entire population of cells, and fluorescent DAPI, which stains non-viable cells, respectively. (aquaanalytics-tekhnika.ru)
- In this the specimens stained with fluorescent dyes like Auramine, Acridine orange etc. are exposed to the light of shorter wavelength - UV light or X-rays - results in the emission of longer wavelength visible light. (paramedicsworld.com)
- To time, live image resolution of irritation provides been restricted to experimental animal models, with examples such as magnetic particles (iron oxide and gadolinium chelates) for magnetic resonance imaging, fluorescent nanoparticles which can be tagged with aptamers or peptides targeted against cell surface biomarkers, circulating factors or nucleic acid structures, or dyes such as acridine orange, which is usually a known human carcinogen (Joussen et al. (uberbrain.net)
Nucleic acid2
- Acridine orange is an organic compound that serves as a nucleic acid-selective fluorescent dye with cationic properties useful for cell cycle determination. (wikipedia.org)
- The maximum excitation and emission value that occur when acridine orange is bound to RNA are the result of electrostatic interactions and the intercalation between the acridine molecule and nucleic acid-base pairs present within RNA and DNA. (wikipedia.org)
Assay1
- Bodipy ceramide labeling (to assay neuroepithelial cell shapes) and acridine orange labeling (to assay cell death) of explants and embryos were performed essentially as described (Brand et al. (techfromastranger.com)
Fragmentation2
- IBMX (600 μM) was shown to induce fragmentation in 661W cells stained with 10-N-nonyl acridine orange (NAO), an acridine orange derivative that marks the inner mitochondrial membrane in whole cells. (molvis.org)
- Ram´s sperm DNA integrity was assessed by SCD (Sperm Chromatine Dispersion) test to study DNA fragmentation and Acridine orange staining was used to estimate DNA denaturation (double-strand DNA versus single-strand DNA). (ac.ir)
Apoptotic cells1
- Acridine orange is able to withstand low pH environments, allowing the fluorescent dye to penetrate acidic organelles such as lysosomes and phagolysosomes that are membrane-bound organelles essential for acid hydrolysis or for producing products of phagocytosis of apoptotic cells. (wikipedia.org)
Fluorescent dye1
- When acridine orange associates with RNA, the fluorescent dye experiences a maximum excitation shift from 525 nm (green) to 460 nm (blue). (wikipedia.org)
Binds3
- When acridine orange binds to DNA, the dye exhibits a maximum excitation at 502 nm producing a maximum emission of 525 nm. (wikipedia.org)
- Nonyl Acridine Orange (NAO) is another potential-independent mitochondrial dye that binds specifically to cardiolipin in the inner mitochondrial membrane. (biotium.com)
- The dye Acridine Orange then binds to the double stranded DNA (dsDNA) and single stranded DNA (ssDNA) and fluoresces green or red respectively. (andrologycenter.in)
Cationic1
- The ability to penetrate the cell membranes of acidic organelles and cationic properties of acridine orange allows the dye to differentiate between various types of cells (i.e., bacterial cells and white blood cells). (wikipedia.org)
Apoptosis3
- Morphological changes of SGNs were observed by acridine orange cytochemistry staining, and apoptosis was examined by flow cytometry. (nih.gov)
- Treatment of SGNs with ONOO(-) resulted in the occurrence of caspase-independent apoptosis as evidenced by acridine orange staining and flow cytometry analysis. (nih.gov)
- Concerning the phenotypic apoptotic changes that were analyzed by Acridine orange and eosin and hematoxylin, Ag NPs caused the apoptosis and Vacuole degeneration as well as cell formation and the emergence of Necrotic cells in MCF-7 cells, whereas in the normal cell line Vero, no change appears in its phenotype. (springeropen.com)
Epifluorescence microscopy2
- Acridine orange is used in epifluorescence microscopy and flow cytometry. (wikipedia.org)
- Acridine orange has been widely accepted and used in many different areas, such as epifluorescence microscopy, and the assessment of sperm chromatin quality. (wikipedia.org)
Bacteria8
- Acridine has antimicrobial factors useful in drug-resistant bacteria and isolating bacteria in various environments. (wikipedia.org)
- Acridine orange in the mid-twentieth century was used to examine the microbial content found in soil and direct counts of aquatic bacteria. (wikipedia.org)
- Additionally, the method of acridine orange direct count (AODC) proved useful in the enumeration of bacteria found within landfills. (wikipedia.org)
- The use of acridine orange in clinical applications has become widely accepted, mainly focusing on highlighting bacteria in blood cultures. (wikipedia.org)
- However, using the Smithwick method, which employs acridine orange as the specific dye, the M. tuberculosis bacteria glow yellow under ultraviolet light microscopy. (cdc.gov)
- Control experiments (obtained by acridine orange direct counting) showed that less than 1% of the bacteria passed through the GF/F filters. (harvard.edu)
- This discrepancy is particularly dramatic in some aquatic environments, in which plate counts and viable cells estimated by acridine orange staining can differ by four to six orders of magnitude (66), and in soil, in which 0.1 to 1% of bacteria are readily culturable on common media under standard conditions (138, 139). (blogspot.com)
- The density of sperm during such events can be determined by directly counting numbers of spermatozoa using a modification of the acridine orange direct count (AODC) technique for enumerating bacteria. (nova.edu)
Fluorescein2
- When bound to DNA, acridine orange is very similar spectrally to an organic compound known as fluorescein. (wikipedia.org)
- Acridine orange and fluorescein have a maximum excitation at 502nm and 525 nm (green). (wikipedia.org)
Detection5
- Acridine orange is recommended for the use of fluorescent microscopic detection of microorganisms in smears prepared from clinical and non-clinical materials. (wikipedia.org)
- IMSEAR at SEARO: Acridine orange stained blood wet mounts for fluorescent detection of malaria. (who.int)
- Hemvani N, Chitnis DS, Dixit DS, Asolkar MV. Acridine orange stained blood wet mounts for fluorescent detection of malaria. (who.int)
- The commercial 'QBC' like acridine orange stained capillary tube preparation has a limitation of precise species identification and the detection of extra-erythrocytic parasites. (who.int)
- Autophagy detection using acridine orange staining Acridine orange staining was used to visualize the volume of the cellular acidic compartment (11). (foodmicro2008.org)
Smears3
- The differential staining capability of acridine orange provides quick scanning of specimen smears at lower magnifications of 400x compared to Gram stains that operate at 1000x magnification. (wikipedia.org)
- The acridine orange stained blood wet mounts over 2420 suspected malaria cases from Indore city were examined under fluorescent microscope and the results compared with the Leishman's stained thick blood smears in a blind study. (who.int)
- The positivity of malarial parasites reported by the modified acridine orange staining was 248 against 109 by Leishman's stained thick blood smears. (who.int)
Fluorochrome1
- Because of this, we looked into its influence on lysosomes using the lysosomotropic fluorochrome, acridine Rabbit Polyclonal to GTPBP2 orange (AO) as well as the acidophilic dye, LysoTracker Crimson (LTR). (gonzalezgass.com)
Intercalation2
- Acridine orange is cell-permeable, which allows the dye to interact with DNA by intercalation, or RNA via electrostatic attractions. (wikipedia.org)
- The results presented (paper-'Study-behavior of HnT with DNA intercalator Acridine Orange') conclude that the degree of intercalation can be reduced in the presence of the HNT setting. (cecon.com)
Parasites1
- Evaluation of acridine-orange staining of centrifuged parasites in malarial infection. (elsevier.com)
Molecule1
- Acridine orange is derived from the organic molecule acridine, which was first discovered by Carl Grabe and Heinrich Caro, who isolated acridine by boiling coal in Germany during the late nineteenth century. (wikipedia.org)
Stains1
- Insets: Acridine orange stains of samples at two distances from the source. (lbl.gov)
Specimens1
- Acridine orange is useful in the rapid screening of ordinarily sterile specimens. (wikipedia.org)
Incubation1
- Nondenaturing gels stained with acridine orange, after incubation in ADP, reveal that the activity producing the poly(A) coelectrophoreses with authentic polynucleotide phosphorylase. (uthscsa.edu)
Lysosomal2
- RESULTS: The results showed that siramesine killed standard glioma cell lines in vitro, and loss of acridine orange staining suggested a compromised lysosomal membrane. (refbase.net)
- Lysosomal membrane integrity, evaluated by acridine orange (AO) staining, was impaired by 40 µM SMA-tDodSNO (P<0.05 vs control) and when combined with SMA-Dox, this effect was significantly potentiated (P<0.001 vs SMA-Dox). (unict.it)
Cells3
- At the appropriate time points following ApoG2 treatment, the cells were incubated with tradition medium comprising 1 mg/ml acridine orange for 15 min. (foodmicro2008.org)
- The cells were then stained with acridine orange for 20 min. (foodmicro2008.org)
- Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. (wikidata.org)
Akridin1
- Resultaten från Halomax® jämfördes med resultaten från mätningar med epi-fluorescens mikroskop och flödescytometri, i båda fallen färgade med akridin orange. (slu.se)
Method3
- Direct epifluorescent filter technique (DEFT) using acridine orange is a method known for examining the microbial content within food and water. (wikipedia.org)
- The modified acridine orange stained method is simple, instant and more efficient, requires less scanning time and skill, allows scanning of larger blood volume (75 ul) at lower magnification and the morphological details at higher magnification helps to make the precise species identification. (who.int)
- Total count was determined using an acridine orange staining method followed by epifluorescence microscopic counting. (cdc.gov)
Tissue1
- Acridine orange-labeled tissue was imaged using epifluorescence on the BX60 microscope and bodipy ceramide-labeled tissue was imaged on an Olympus IX70 microscope equipped with a BioRad Radiance 2000 confocal laser system. (techfromastranger.com)
Clay minerals1
- 2022: Sorption of acridine orange on non-swelling and swelling clay minerals, Crystals (118 pp. (uwp.edu)
Widely1
- This chemical is widely used in processing of xylenlol orange, redox indicator and anti-oxidents. (arnishlaborates.in)
Direct count1
- Metode Total Plate Count digunakan untuk menentukan kandungan bakteri heterotrofik, sedangkan produktivitas bakteri ditentukan dengan metode Acridine Orange Direct Count. (undip.ac.id)
Organelles1
- Systematic colocalization errors between acridine orange and EGFP in astrocyte vesicular organelles. (mpg.de)
Microscope1
- The acridine orange was eliminated and fluorescent micrographs were captured using a DM-IRB inverted fluorescent microscope (Leica, Wetzlar, Germany). (foodmicro2008.org)
Study1
- Carried out a study to understand the conduct of HNT with the DNA intercalator Acridine Orange. (cecon.com)
Blood1
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Wild-type1
- E , F ) Acridine Orange-stained 2 dpf wild-type (E) and gle1 -/- (F) embryos. (zfin.org)
Quality1
- As one of the trusted manufacturer, exporter and supplier of allied domain, we have been engaged in providing our clients with excellent quality ACRIDINE ORANGE ZNCL2 . (arnishlaborates.in)
Release1
- Results: A strong cytolytic effect of the compound was signified by lipid release and acridine orange staining of dying adipocyte nuclei. (mdc-berlin.de)