A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.
A plant species of the genus CITRUS, family RUTACEAE that provides the familiar orange fruit which is also a source of orange oil.
Acridines which are substituted in any position by one or more amino groups or substituted amino groups.
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide.
Agents that are capable of inserting themselves between the successive bases in DNA, thus kinking, uncoiling or otherwise deforming it and therefore preventing its proper functioning. They are used in the study of DNA.
PHENOTHIAZINES with an amino group at the 3-position that are green crystals or powder. They are used as biological stains.
Plasmids which determine the ability of a bacterium to ferment lactose.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
A dye that is a mixture of violet rosanilinis with antibacterial, antifungal, and anthelmintic properties.
A plant genus of the family RUTACEAE. They bear the familiar citrus fruits including oranges, grapefruit, lemons, and limes. There are many hybrids which makes the nomenclature confusing.
Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.
Acidic phospholipids composed of two molecules of phosphatidic acid covalently linked to a molecule of glycerol. They occur primarily in mitochondrial inner membranes and in bacterial plasma membranes. They are the main antigenic components of the Wassermann-type antigen that is used in nontreponemal SYPHILIS SERODIAGNOSIS.
A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
An aniline dye used as a disinfectant and an antiseptic agent. It is weakly fluorescing and binds specifically to certain proteins.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A highly fluorescent anti-infective dye used clinically as a topical antiseptic and experimentally as a mutagen, due to its interaction with DNA. It is also used as an intracellular pH indicator.
A neoplasm containing HISTIOCYTES. Important forms include BENIGN FIBROUS HISTIOCYTOMA; and MALIGNANT FIBROUS HISTIOCYTOMA.
Measurement of light given off by fluorescein in order to assess the integrity of various ocular barriers. The method is used to investigate the blood-aqueous barrier, blood-retinal barrier, aqueous flow measurements, corneal endothelial permeability, and tear flow dynamics.

Comparison of five methods of malaria detection in the outpatient setting. (1/413)

In eastern Africa where 90% of the malaria is due to Plasmodium falciparum, the accuracy of malaria diagnosis at the outpatient level is becoming increasingly important due to problems of drug resistance and use of alternative, costly antimalarial drugs. The quantitative buffy coat (QBC) technique, acridine orange staining with an interference filter system, and the ParaSight-F test have been introduced as alternative methods to conventional microscopy for the diagnosis of malaria. Two hundred thirteen outpatients were tested using these alternative methods and conventional microscopy by five experienced technologists; two were randomly allocated to read the results of each test. Paired results showed the highest level of agreement with the ParaSight-F test (99%), followed by Field stain (92%). The results of the QBC technique showed the least agreement (73%). Using conventional microscopy as the reference standard, the ParaSight-F test had a sensitivity range of 90-92% and a specificity of 99%, staining with acridine orange had a sensitivity range of 77-96% and a specificity range of 81-98% and the QBC technique had a sensitivity range of 88-98% and a specificity range of 58-90%. All microscopic tests showed lower sensitivities (as low as 20% using staining with acridine orange) in detecting low parasitemias (< or = 320/microl) than the ParaSight-F test (70%). Due to the high cost of the ParaSight-F test, Field-stained blood films remain the most appropriate method for diagnosis of P. falciparum in eastern Africa. The ParaSight-F test may be used in situations where no trained microscopists are available, or where malaria is strongly suspected and the results of microscopy are negative.  (+info)

Destruction of protamine in human sperm inhibits sperm binding and penetration in the zona-free hamster penetration test but increases sperm head decondensation and male pronuclear formation in the hamster-ICSI assay. (2/413)

PURPOSE: Our purpose was to investigate the fertilizing ability of human protamine-damaged sperm in a heterologous system using hamster oocytes. METHODS: The protamine of the sperm were damaged by exposure to dithiothreitol, a disulfide-reducing agent. Their ability to penetrate and form male pronuclei were investigated using the zona-free hamster penetration test and the hamster-intracytoplasmic sperm injection assay, respectively. RESULTS: The zona-free hamster penetration test revealed that protamine-damaged sperm are unable to bind and penetrate the hamster oocyte. On the other hand, hamster-intracytoplasmic sperm injection assay results showed that 56.9% and 39.2% of the injected oocytes developed male pronuclei in protamine-damaged and live-intact sperm groups, respectively, with a significant difference in these rates (P < 0.01). CONCLUSIONS: This study shows that protamine-damaged sperm are able to undergo sperm head decondensation and male pronuclear formation only when injected into the ooplasm, although they cannot bind and penetrate through the zona and enter the ooplasm.  (+info)

Reactive oxygen species-induced apoptosis and necrosis in bovine corneal endothelial cells. (3/413)

PURPOSE: The loss of corneal endothelial cells associated with aging and possibly other causes has been speculated to be related to exposure to reactive oxygen species (ROS). The current study was conducted to investigate, by use of photosensitizers, the underlying mechanisms involved in the death of bovine corneal endothelial cells (BCENs) caused by ROS. METHODS: BCEN cells in primary culture were treated with a photosensitizer (riboflavin or rose bengal) with light exposure. The patterns of cell damage and death were assessed using an acridine orange-ethidium bromide differential staining method, TdT-mediated dUTP nick-end labeling (TUNEL) assay, and transmission electron microscopy. The cytotoxicity was assayed by mitochondrial function using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) testing. Antioxidants, including catalase, L-histidine, salicylic acid, and superoxide dismutase, were used to determine the types of ROS involved. Activation of nuclear factor (NF)-kappaB was examined by fluorescent immunocytochemistry with anti-p65 antibody. RESULTS: Light-irradiated riboflavin or rose bengal resulted in a significant decrease in viability of BCEN cells. Chromosomal condensation and fragmentation were observed in apoptotic cells, and membrane lysis and damage of cell ultrastructures were observed in necrotic cells. Riboflavin induced apoptosis at 30 minutes and thereafter and induced necrosis after 2 hours. Rose bengal was shown to cause similar effects within half the time required for the effects of riboflavin. Catalase and salicylic acid were found to provide protection for BCENs from cytotoxic effects of riboflavin, and L-histidine was found to protect BCENs from cytotoxicity induced by rose bengal. Kinetic studies using immunocytochemistry showed that NF-kappaB was translocated into the nucleus within 15 minutes and 30 minutes after treatment with rose bengal and riboflavin, respectively. CONCLUSIONS: The cytotoxic effects of photo-irradiated riboflavin and rose bengal are shown to be mediated by two distinct but parallel pathways, one leading to apoptosis and the other to necrosis. Possible involvement of NF-kappaB in cell death is suggested. These findings provide potential leads for future investigation into the molecular mechanisms of loss of corneal endothelial cells related to aging, oxidative stress, and possibly other similar causes.  (+info)

Differential chemosensitivity of breast cancer cells to ganciclovir treatment following adenovirus-mediated herpes simplex virus thymidine kinase gene transfer. (4/413)

The development of resistance to radiation and chemotherapeutic agents that cause DNA damage is a major problem for the treatment of breast and other cancers. The p53 tumor suppressor gene plays a direct role in the signaling of cell cycle arrest and apoptosis in response to DNA damage, and p53 gene mutations have been correlated with increased resistance to DNA-damaging agents. Herpes simplex virus thymidine kinase (HSV-tk) gene transfer followed by ganciclovir (GCV) treatment is a novel tumor ablation strategy that has shown good success in a variety of experimental tumor models. However, GCV cytotoxicity is believed to be mediated by DNA damage-induced apoptosis, and the relationship between p53 gene status, p53-mediated apoptosis, and the sensitivity of human tumors to HSV-tk/GCV treatment has not been firmly established. To address this issue, we compared the therapeutic efficacy of adenovirus-mediated HSV-tk gene transfer and GCV treatment in two human breast cancer cell lines: MCF-7 cells, which express wild-type p53, and MDA-MB-468 cells, which express high levels of a mutant p53 (273 Arg-His). Treating MCF-7 cells with AdHSV-tk/GCV led to the predicted increase in endogenous p53 and p21WAF1/CIP1 protein levels, and apoptosis was observed in a significant proportion of the target cell population. However, treating MDA-MB-468 cells under the same conditions resulted in a much stronger apoptotic response in the absence of induction in p21WAF1/CIP1 protein levels. This latter result suggested that HSV-tk/GCV treatment can activate a strong p53-independent apoptotic response in tumor cells that lack functional p53. To confirm this observation, four additional human breast cancer cell lines expressing mutant p53 were examined. Although a significant degree of variability in GCV chemosensitivity was observed in these cell lines, all displayed a greater reduction in cell viability than MCF-7 or normal mammary cells treated under the same conditions. These results suggest that endogenous p53 status does not correlate with chemosensitivity to HSV-tk/GCV treatment. Furthermore, evidence for a p53-independent apoptotic response serves to extend the potential of this therapeutic strategy to tumors that express mutant p53 and that may have developed resistance to conventional genotoxic agents.  (+info)

Evanescent-wave microscopy: a new tool to gain insight into the control of transmitter release. (5/413)

Evanescent-wave excitation was used to visualize individual fluorescently labelled vesicles in an optical slice near the plasma membrane of bovine adrenal chromaffin cells. A standard upright microscope was modified to accommodate the optics used for directing a laser beam under a supracritical angle on to the glass-water interface on top of which the cells are grown. Whereas epi-illumination images appeared blurred and structureless, evanescent-wave excitation highlighted acridine orange-labelled vesicles as individual pinpoints. Three-dimensional (3D) trajectories of individual vesicles were obtained from time-resolved image stacks and used to characterize vesicles in terms of their average fluorescence F and mobility, expressed here as the 3D diffusion coefficient D(3). Based on the single-vesicle analysis, two groups of vesicles were identified. Transitions between these states were studied before and after stimulation of exocytosis by repetitive or maintained membrane depolarizations by elevated extracellular [K+]. Findings were interpreted as sequential transitions between the previously characterized pools of vesicles preceding the fusion step. The observed approach of vesicles to their docking sites was not explained in terms of free diffusion: most vesicles moved unidirectionally as if directed to their binding sites at the plasma membrane. Vesicle mobility at the membrane was low, such that the sites of docking and fusion were in close vicinity. Both the rim region and confined areas in the centre of the footprint region were the site of intense vesicle trafficking.  (+info)

Arrest of endosome acidification by bafilomycin A1 mimics insulin action on GLUT4 translocation in 3T3-L1 adipocytes. (6/413)

In insulin-sensitive fat and muscle cells, the major glucose transporter GLUT4 is constitutively sequestered in endosomal tubulovesicular membranes, and moves to the cell surface in response to insulin. While sequence information within GLUT4 appears to be responsible for its constitutive intracellular sequestration, the regulatory elements and mechanisms that enable this protein to achieve its unique sorting pattern under basal and insulin-stimulated conditions are poorly understood. We show here that arrest of endosome acidification in insulin-sensitive 3T3-L1 adipocytes by bafilomycin A1, a specific inhibitor of the vacuolar proton pump, results in the rapid and dose-dependent translocation of GLUT4 from the cell interior to the membrane surface; the effects of maximally stimulatory concentrations of bafilomycin A1 (400-800 nM) were equivalent to 50-65% of the effects of acute insulin treatment. Like insulin, bafilomycin A1 induced the redistribution of GLUT1 and Rab4, but not that of other proteins whose membrane localization has been shown to be insulin-insensitive. Studies to address the mechanism of this effect demonstrated that neither autophosphorylation nor internalization of the insulin receptor was altered by bafilomycin A1 treatment. Bafilomycin-induced GLUT4 translocation was not blocked by cell pretreatment with wortmannin. Taken together, these data indicate that arrest of endosome acidification mimics insulin action on GLUT4 and GLUT1 translocation by a mechanism distal to insulin receptor and phosphatidylinositol 3-kinase activation, and suggest an important role for endosomal pH in the membrane dynamics of the glucose transporters.  (+info)

Radiation-induced leukocyte entrapment in the rat retinal microcirculation. (7/413)

PURPOSE: To evaluate the effects of irradiation on leukocyte dynamics in the rat retinal microcirculation. METHODS: Thirty-five Brown-Norway rats received a dose of 10 Gy irradiation in one fraction. Leukocyte dynamics were studied with acridine orange digital fluorography, in which a nuclear fluorescent dye of acridine orange is used and examined by scanning laser ophthalmoscope. This technique allowed visualization of fluorescent leukocytes in vivo. The leukocyte dynamics were evaluated at 0, 4, 7, 14, 30, and 60 days after the irradiation. RESULTS: Mean leukocyte velocity in the retinal capillaries decreased immediately. It partially recovered on day 4 but then gradually decreased up to the 2-month mark. Low-dose irradiation induced entrapment of leukocytes in the retinal microcirculation. The number of entrapped leukocytes gradually increased with time. The major retinal vessels significantly constricted immediately after irradiation. The diameter was reduced by 76% in arteries and 75% in veins, 2 months after irradiation. CONCLUSIONS: Entrapped leukocytes may be activated and exacerbate vascular injury and microinfarction and thus may participate in the pathogenesis of radiation retinopathy.  (+info)

Tick-borne relapsing fever imported from West Africa: diagnosis by quantitative buffy coat analysis and in vitro culture of Borrelia crocidurae. (8/413)

West African tick-borne relapsing fever (TBRF) is difficult to diagnose due to the low number of spirochetes in the bloodstream of patients. Previously, the causative microorganism, Borrelia crocidurae, had never been cultured in vitro. TBRF was rapidly diagnosed for two patients returning from western Africa with fever of unknown origin by quantitative buffy coat (QBC) analysis. Diagnosis was confirmed by intraperitoneal inoculation of blood specimens from patients into laboratory mice. In vitro experiments showed that QBC analysis may be as much as 100-fold more sensitive than thick smear. Spirochetes were also cultured from blood samples from both patients in modified Kelly's medium and were identified as B. crocidurae by partial sequencing of the PCR-amplified rrs gene.  (+info)

Purified Mitochondrial Membrane Potential Assay Kit (Orange Fluorescence) from Creative Biomart. Mitochondrial Membrane Potential Assay Kit (Orange Fluorescence) can be used for research.
Purified Fixable Dead Cell Staining Kit (Orange Fluorescence 405) from Creative Biomart. Fixable Dead Cell Staining Kit (Orange Fluorescence 405) can be used for research.
Components: s2146 Acridine Orange Stock Solution s1656 Sorensens Phosphate Buffer pH 6.0 s2147 Calcium Chloride 0.1M Solution s100 Acetic Acid 1% Aqueous
For speedy, intra-operative pathological margin assessment to steer staged cancers excisions, multimodal confocal mosaic check picture wide surgical margins (approximately 1?cm) with sub-cellular resolution and mimic the appearance of conventional hematoxylin and eosin histopathology (H&E). The goal of this work is definitely to combine three confocal imaging modes: acridine orange fluorescence (AO) for labeling nuclei, […]. ...
We do routinely use acridine orange using FACScan. A 20 min rinse with 10 times water-diluted Chlorox bleach is adequate to clean the instrument. Zbigniew Darzynkiewicz -----Original Message----- From: Karen Creswell [mailto:creswelk at georgetown.edu] Sent: Friday, June 04, 2004 1:17 PM To: cyto-inbox Subject: Acridine Orange Does anyone routinely run acridine orange through their FACScan/FACSort? I would like suggestions for cleaning the instrument afterwards. Thanks for your help, Karen -- Karen Creswell, Ph.D. Director, Flow Cytometry/Cell Sorting Shared Resource Lombardi Cancer Center Georgetown University Medical Center Research Bldg W304 Box 571469 Washington, DC 20057-1469 202-687-2233 creswelk at georgetown.edu ...
Acridine Orange hemi(zinc chloride) salt from Sigma-Aldrich,Application: Acridine orange has been used as a fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Acridine orange at a concentration of 120 μM will detect purified DNA in gels with a sensitivity of 25-50 ng per band. It has also been used extensively for cell staining of,biological,biology supply,biology supplies,biology product
The adsorption of the metachromic dye acridine orange (AO) by Na-beidellite was investigated by visible spectroscopy. Different types of clay-AO association were identified from the appearance and location of absorption bands alpha or beta . The colloidal properties were determined from curves of the absorbance vs. the degree of saturation. Three regions were identified in the absorbance curve. In the first region beidellite is peptized with small amounts of AO and the dye penetrates into the interlayer space where it undergoes metachromasy due to pi interactions between the aromatic entity and the oxygen plane of the clay. With larger amounts of AO (second region), the clay flocculates due to the aggregation of the dye cations in the interparticle space of the flocs. In excess AO (third region), beidellite is gradually peptized, forming small tactoids with monomeric AO in the interlayer space and at the same time adsorbing dimeric and polymeric AO cationic species at the solid-liquid interface. ...
Sixteen marine isolates from a NORPAX cruise, which were transferred once on medium after initial isolation, survived nutrient deprivation for at least 8 months (longest period test). All but one isolate remained cellularly intact, although their sizes and shapes changed greatly, and all became smaller, decreasing in size from 40 to 79%. Three starvation-survival patterns were demonstrated, namely (i) an initial increase in viable cells followed by a decrease until a constant number was reached, (ii) an increase in viable cells until a constant number was reached, and (iii) a decrease in viable cells until a constant number was reached. One isolate from each starvation-survival pattern was starved for 8 months and then was tested in comparison with 4-month-starved Ant-300 for [14C]glutamic acid uptake, respiration, and incorporation. The response to glutamic acid was rapid and linear in each case. The data indicate that the starvation-survival of Ant-300 is not an anomalous situation and that ...
BioAssay record AID 693243 submitted by ChEMBL: Induction of apoptosis in human MGC803 cells assessed as pyknosis at 5 uM after 12 to 48 hrs by acridine orange/ethidium bromide staining.
Acridine Orange: A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.
DNA intercalating dye. A grade of acridine orange of exceptionally high purity, suitable for quantitative work. Free of inorganic salts.
HeLa cells. Light micrograph (acridine orange stain) of a culture of HeLa cancer cells. The nuclei of the cells are stained yellow while the cytoplasm is orange. HeLa cells are the first human cell line established in 1952 to research cancer. They were obtained from the cervix of Henrietta Lacks (after whom the cells are named) from Baltimore, USA, in 1951, who died of cervical cancer eight months later. HeLa cells thrive unusually well in the laboratory and are used in research worldwide. Professor Anderson (1911-2006) was the first to use acridine orange staining to study cells, bacteria and viruses. - Stock Image G442/0059
Background: Mitochondrial permeabilization provokes cardiomyocyte death, and causes adverse left ventricular remodeling in cardiac-restricted TNF transgenic mice (MHCsTNF). Autophagy, a lysosomal degradative pathway, is essential for removal of ubiquitin-tagged damaged mitochondria. Our prior work suggests that TNF receptor associated factor-2 (TRAF2), a multifunctional protein with E3 ubiquitin ligase activity, facilitates removal of damaged mitochondria. The role of TRAF2 vis-à-vis PARKIN, another E3 ubiquitin ligase essential for mitophagy, is unknown.. Methods and Results: MHCsTNF hearts and TNF-treated neonatal rat cardiac myocytes (NRCMs) demonstrate upregulation of both TRAF2 and PARKIN, with increased evidence for mitochondrial autophagy and reduced mitochondrial mass. To evaluate the role of TRAF2 and PARKIN in autophagic removal of damaged mitochondria, we employed gain-of-function and loss-of-function approaches; and assessed mitochondrial mass with nonyl-acridine orange fluorescence ...
Stain F-actin in cell samples with F-actin Staining Kit - Orange Fluorescence - Cytopainter ab112126. For fluorescence microscopes.
A while ago, scientists at the National University of Singapore created transgenic, fluorescent zebra fish by injecting red, green, and orange fluorescence genes from a jellyfish into the fish eggs. They did this so the fish could help detect environmental pollutants.Now, you can actually have them for pets! They are marketed under the name GloFish, and look very cool under a black light. Link - Thanks moniA!Previously on Neatorama: Fluorescent pig, rabbit, butterfly, and frog...
The metal chelating compound Dp44mT is a di-2-pyridylketone thiosemicarbazone (DpT) which displays potent and selective anti-tumor activity. This compound is receiving translational attention but its mechanism is poorly understood. Here we report that Dp44mT targets lysosome integrity through copper binding. Studies using the lysosomotropic fluorochrome acridine orange established that the copper-Dp44mT complex (Cu[Dp44mT]) disrupted lysosomes. This targeting was confirmed with pepstatin A-BODIPY FL, which showed re-distribution of cathepsin D to the cytosol with ensuing cleavage of the pro-apoptotic BH3 protein Bid. Redox activity of Cu[Dp44mT] caused cellular depletion of glutathione and lysosomal damage was prevented by co-treatment with the glutathione precursor N-acetylcysteine. Copper binding was essential for the potent anti-tumor activity of Dp44mT, since co-incubation with non-toxic copper chelators markedly attenuated its cytotoxicity. Taken together, our studies show how the lysosomal ...
The majority of phosphatase (PO4ase) activity detected in fresh aerobic activated sludge from a municipal wastewater treatment plant was associated with suspended floc material. PO4ase activity appeared to be localized in discrete bacteria-containing areas of the floc matrix based on the distribution of nucleic acid-stained cells and precipitated fluorescent crystals produced as a result of reaction of the enzyme(s) with the artificial substrate ELF™-PO4. Of the total floc-associated bacterial cells that stained positive with the nucleic acid-binding fluorochrome acridine orange (AO), 8.8 ± 1.2% displayed PO4ase activity based on the proximity of AO-stained cells to precipitated ELF crystals. Using a 16S rRNA oligonucleotide probe specific for the cytophaga-flavobacteria group, it was determined that 17-20% of the floc-associated bacteria that probed positive also displayed PO4ase activity. Furthermore, 35-45% of the ELF fluorescence was associated with bacterial cells that probed positive ...
Name:C.I.Basic Orange 14,C.I.46005 Molecular Structure: Acridines class C.I.Basic Orange 14,C.I.46005,CAS 65-61-2,301.81,C17H20ClN3,Acridine Orange C.I.Basic Orange 14,C.I.46005,CAS 65-61-2,301.81,C17H20ClN3,Acridine Orange Molecular Formula:C17H20ClN3 Molecular Weight: 301.81 CAS Registry Number:65-61-2
Aims: In view of recent findings that a multidrug efflux pump CmeABC exists in Campylobacter jejuni, 391 C. jejuni and 52 Campylobacter coli of human and animal origin were examined for a multidrug resistance phenotype.. Materials and methods: The MICs of ampicillin, chloramphenicol, ciprofloxacin, erythromycin, kanamycin, tetracycline, cetrimide, triclosan, acridine orange, paraquat and ethidium bromide were determined. Resistance to organic solvents and the effect of salicylate (known inducer of the marRAB operon in Escherichia coli and Salmonella) were also examined.. Results: Two C. coli and 13 C. jejuni isolates, mainly from pigs or poultry, were resistant to three or more antibiotics and 12 of these strains had reduced susceptibility to acridine orange and/or ethidium bromide. Strains (n = 20) that were less susceptible to acridine orange, ethidium bromide and triclosan were significantly more resistant (P , 0.05) to ampicillin, chloramphenicol, ciprofloxacin, erythromycin, nalidixic acid ...
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Acridine orange is a nucleic acid staining dye that is used to identify dead cells. It intercalates with the double-stranded DNA base pairs at a ratio of 1:3 and is capable of emitting green fluorescence (Ex: 500 nm, Em: 520 nm). It also emits red fluorescence (Ex: 460 nm, Em: 650 nm) when bound to RNA or single-stranded DNA. Since acridine orange is mutagenic, this product is supplied as a solution that prevents it from splashing during weighing.. ...
During human spermiogenesis, the elongated spermatids undergo a plasma membrane remodeling step that facilitates formation of the zona pellucida and hyaluronic acid (HA) binding sites. Various biochemical sperm markers indicated that human sperm bound to HA exhibit attributes similar to that of zona …
The accuracy of techniques for the diagnosis of malaria are usually compared with optical microscopy, which is considered to be a gold standard. However, microscopy is prone to error and therefore makes it difficult to assess the reliability of other diagnostic techniques. We did a systematic review to assess the specificity and sensitivity of diagnostic techniques in different settings, using a statistical method that avoided defining a gold standard. Performance varied depending on species of the malaria parasite, level of parasitaemia, and immunity. Overall, histidine-rich protein 2 (HRP2)-based dipsticks showed a high sensitivity (92.7%) and specificity (99.2%) for Plasmodium falciparum in endemic areas. The acridine orange test was more sensitive (97.1%) in detecting P falciparum in epidemiological studies, with a specificity of 97.9%. In the absence of a gold standard, HRP2 dipsticks and acridine orange could provide an alternative for detecting falciparum infections in endemic areas and
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acridine 260-94-6 MSDS report, acridine MSDS safety technical specifications search, acridine safety information specifications ect.
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PromoKine offers a wide range of dyes for fluorescently staining dead cells. The membrane-impermeable nuclear stains can also be used for fixed cells. Commonly used dyes for staining dead cells include; orange-fluorescent ethidium bromide (EB), red-fluorescent propidium iodide (PI), green-fluorescent acridine orange (AO) and blue-fluorescent DAPI. The toluidine derivative Trypan Blue is a classical non-fluorescent dye used to selectively stain dead cells and shows a distinctive blue colour under the microscope. ...
A quiescent [denoted as Q(G0/G1)] subpopulation was isolated from EMT6/Ro-fed plateau monolayers by centrifugal elutriation. The median Coulter volume of these cells was significantly smaller than that of the original population from which they were elutriated. Using two-step acridine orange staining and dual parameter flow cytometric analysis, over 95% of quiescent cells were found to have G1 DNA content, and 80% of the cells had a decreased RNA content as compared to rapidly proliferating exponential G1 cells. After labeling for 24 hr (two doubling times) with [3H]thymidine, less than 2% of the quiescent cells incorporated [3H]thymidine as measured by autoradiography. The colony-forming efficiency of these cells was not significantly different from that of exponential cells. When such Q(G0/G1) cells were replated in fresh medium at a lower density, there was a lag time of 30 hr before any increase in cell number was detected, after which the cell-doubling rate matched that of exponential ...
contact Luis Benitez at Oncological Research Unit, kAP 107-014, UNAM, Hosp. 21 Sieglo, Mexico DF 06700, Mexico. He has some interesting results along this line. In article ,905kf544 at invrof.anmat.sld.ar, elisa at invrof.anmat.sld.ar (Dra. Elisa Bal de Kier-Joffe) writes: ,Dear netters: ,We are using Acridine Orange (AO) to stain apoptotic cells. , ,The nuclei staining of apoptotic cells is very easily recognized when it is ,in an advanced stage. ,However when the process is early I observe only a brighter staining of ,chromatin clumps, and a more marked limit in nuclei periphery. When this ,happens, the cytoplasm shows a diffuse red staining which is also evident in ,definite apoptotic cells. , ,In contrast normal alive cells only show a pale diffuse green staining of ,the nuclei and cytoplasm and only a kind of red granule (RNA) in the ,cytoplasm next to the nucleus. , ,I have not found any comments concerning this early diffuse intense red ,staining of the cytoplasm with AO in apoptotic ...
A novel method for the detection, screening, the quantitation of compounds that interact with nucleic acids (DNA or RNA) is provided. The basis for this method is the inhibition of acridine orange binding to the nucleic acid. Measurement of binding inhibition utilizes the rapid, repeatable technique of fluorescence polarization.
Fluorescence filter set EX480 (BP30) DM505(LP) EM535 (BP40) optimized for Acridine Orange + DNA, Alexa Fluor 488™, Atto 488, Azami Green, BODIPY FL/pH7.2, Calcein, Calcium, Green™-1, Cy2™, DiO, DyLight 488, EGFP, Emerald GFP, FAM, FITC, Fluo-4, GFP, LysoTracker Green/pH 5.2, MitoTracker Green, FM/MeOH, mWasabi, Oregon Green™ 488, SYBR® Green I, SYTO 9/DNA, ZsGreen fluorophore. Needs empty filter block (OX.9749 ...
Fluorescent micrographs and quantitative analysis of CAOV-3 cells double-stained with acridine orange and propidium iodide. Cells were treated with liriodenine
This month the Artist Inspired theme is Orange. Like pink, orange is not one of my favorite colors. For a person whose wardrobe consists predominately of black, burgandys and blue, orange is kind of bright for me. So what do I think of when I see the topic orange. Since moving to the USA, orange…
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To peel the oranges cut both ends and place the orange on one end on a cutting board. Using a sharp knife shave the peel off downwards all the way around, making sure to also remove the thin white skin around the orange. Chop the orange into small pieces. Weigh the chopped orange. ...
VFOG perf 8.5 coil stamps of the 1c orange, bright orange & pale bright orange King George V from the 1930-1935 Arch Issue. With cream gum with a semi-gloss sheen & horizontal striations. Although not listed specifically by Unitrade, the stamps of this issue exist with several different gum types, as well as sh
17784-47-3 - XUESTGHCVFYOLL-UHFFFAOYSA-N - Acridine, hydrochloride - Similar structures search, synonyms, formulas, resource links, and other chemical information.
Wear an orange ribbon, and youre helping spread the word about leukemia, multiple sclerosis, kidney cancer, kidney disease, gun violence and other orange ribbon awareness projects. We offer a full line of orange ribbon products, from bracelets to lanyards, so theres merchandise to fit every fundraiser. With our rock-bottom prices and high quality products, you can buy with complete confidence. Order before 3:00 pm EST, and youll qualify for Same Day Shipping! If you are shopping for just one or a few items, please click over to our Orange Ribbon Gift Shop.. ...
Amazing taste of oranges and fluffy texture of cupcakes are irresistible! We cant go without oranges in winter, as they are very tasty and we are obsessed with the wonderful taste and smell. The bonus is that they are also very healthy, as they contain a huge amount of vitamins , the consumption of which is especially important in winter, because they boost the immune system. We just had to make some delicious dessert out of them and since we hadnt made cupcakes in quite a long time, it seemed to us that it was really time to bake them. We can say that orange cupcakes are one of the best desserts we have baked lately! And not only are they extremely tasty, the preparation is also very simple, so anyone can make them.. Cupcakes are a great breakfast and go well with a cup of coffee or tea, but you can also take them with you to work or school and enjoy the refreshing taste of oranges. These cupcakes contain very small amount of sugar and oil, so they are a great alternative to store bought ...
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Fruit can be divided into a good many varieties; especially oranges are popular fruit. Oranges are eaten by many ways; such as the orange drink. The process of manufacturing orange drink can be divided into four stages: picking and preparation of the f...
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The 2021 Nissan Juke Ti (ENERGY ORANGE) is a front-wheel drive five-door hatchback that was released to the Australian market on 01/06/2021 classified as a F16 MY21. The Juke is regarded as a SUV light built in Japan with prices starting at A$36,490 before on-road costs. The Juke is a front-wheel drive 5 door with 5 seats, powered by a 1.0L TURBO 3 engine that has 84 kW of power (at 5250 rpm) and 180 Nm of torque (at 2400 rpm) via a Seven-speed Auto Dual Clutch. NISSAN claims the Juke Ti (ENERGY ORANGE) uses 5.8L/100km of Premium Unleaded Petrol in the combined city and highway cycle while putting out 136g of CO2. It has a 46L fuel tank, meaning it should be able to travel 793km per full tank. The Juke measures 1595mm (62.8 inches) in height, 4210mm (165.7 inches) in length, 1800mm (70.9 inches) in width with a 2636mm (103.8 inches) wheelbase that brings about a total of 1274kg (2808.7 lbs) of unladen weight. The Juke Ti (ENERGY ORANGE) comes standard with P225/45 R19 front tyres and P225/45 R19 rear
Im excited by the opportunity to work for a business that has fostered an unprecedented level of cross discipline collaboration with each employee feeling empowered to contribute their ideas and solutions.. Im joining at a time when Orange Line are ready to take these solutions to market in Australia and beyond, Farrell said in a statement.. Orange Line has also appointed Stephanie Ford as an operations manager.. Ford joins the agency from ReachLocal.. David Klein, co-founder and director at Orange Line, said in a statement: The appointments of both Aishling and Stephanie come at a crucial point for the agency as we ramp up our service offering and expand our operations with clients in overseas markets.. Both have significant expertise and experience in their respective fields and they will be a great addition to the team as we look forward to a bumper 2018.. ...
Deep, Dark Salted Butter Caramel Sauce, for serving (after using Zoe Nathans, decided that I definitely prefer my own, reprinted below). 1. In a food processor, pulse the 1 cup of flour with 2 tablespoons of the sugar and the baking powder and salt. Add the stick of cold butter and pulse several times, just until it is the size of peas. Sprinkle the dough with the ice water and pulse just until moistened crumbs form. Turn the crumbs out onto a work surface, knead once or twice and pat the pastry into a disk. Wrap the pastry in plastic and chill for 30 minutes.. 2. On a floured work surface, roll out the pastry to an 11-inch round, about 1/4 inch thick. Transfer the pastry to a parchment paper-lined flat cookie sheet and refrigerate for 15 minutes, or until chilled.. 3. Meanwhile, peel the blood oranges, removing all of the bitter white pith. Thinly slice 2 of the oranges crosswise; remove the pits. Transfer the orange slices to a plate. Working over a sieve set over a bowl, cut in between the ...
Deep, Dark Salted Butter Caramel Sauce, for serving (after using Zoe Nathans, decided that I definitely prefer my own, reprinted below). 1. In a food processor, pulse the 1 cup of flour with 2 tablespoons of the sugar and the baking powder and salt. Add the stick of cold butter and pulse several times, just until it is the size of peas. Sprinkle the dough with the ice water and pulse just until moistened crumbs form. Turn the crumbs out onto a work surface, knead once or twice and pat the pastry into a disk. Wrap the pastry in plastic and chill for 30 minutes.. 2. On a floured work surface, roll out the pastry to an 11-inch round, about 1/4 inch thick. Transfer the pastry to a parchment paper-lined flat cookie sheet and refrigerate for 15 minutes, or until chilled.. 3. Meanwhile, peel the blood oranges, removing all of the bitter white pith. Thinly slice 2 of the oranges crosswise; remove the pits. Transfer the orange slices to a plate. Working over a sieve set over a bowl, cut in between the ...
For rapid, intra-operative pathological margin assessment to guide staged cancer excisions, multimodal confocal mosaic scan image wide surgical margins (approximately 1 cm) with sub-cellular resolution and mimic the appearance of conventional hematoxylin and eosin histopathology (H&E). The goal of this work is to combine three confocal imaging modes: acridine orange fluorescence (AO) for labeling nuclei, eosin fluorescence (Eo) for labeling cytoplasm, and endogenous reflectance (R) for marking collagen and keratin. Absorption contrast is achieved by alternating the excitation wavelength: 488 nm (AO fluorescence) and 532 nm (Eo fluorescence). Superposition and false-coloring of these modes mimics H&E, enabling detection of cutaneous squamous cell carcinomas (SCC). The sum of mosaic Eo þ R is false-colored pink to mimic the appearance of eosin, while the AO mosaic is false-colored purple to mimic the appearance of hematoxylin in H&E. In this study, mosaics of 10 Mohs surgical excisions containing ...
For rapid, intra-operative pathological margin assessment to guide staged cancer excisions, multimodal confocal mosaic scan image wide surgical margins (approximately 1 cm) with sub-cellular resolution and mimic the appearance of conventional hematoxylin and eosin histopathology (H&E). The goal of this work is to combine three confocal imaging modes: acridine orange fluorescence (AO) for labeling nuclei, eosin fluorescence (Eo) for labeling cytoplasm, and endogenous reflectance (R) for marking collagen and keratin. Absorption contrast is achieved by alternating the excitation wavelength: 488 nm (AO fluorescence) and 532 nm (Eo fluorescence). Superposition and false-coloring of these modes mimics H&E, enabling detection of cutaneous squamous cell carcinomas (SCC). The sum of mosaic Eo þ R is false-colored pink to mimic the appearance of eosin, while the AO mosaic is false-colored purple to mimic the appearance of hematoxylin in H&E. In this study, mosaics of 10 Mohs surgical excisions containing ...
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Publications and Work Accepted for Publication:. A. Books. - Poli A., Fabbri E., Agnisola C., Calamita G., Santovito G., Verri T. Fisiologia Animale. Edises, Napoli, Italy, pp. 1-633, 2014. - Poli A., Fabbri E., Agnisola C., Calamita G., Santovito G., Verri T. Fisiologia Animale(II edizione).Edises, Napoli, Italy,pp. 1-720, 2018. B. Articles in Scholarly Journals. - Verri T.*, Maffia M., Storelli C. H+/glycyl-glycine cotransport in eel intestinal brush border membrane vesicles: studies with the pH-sensitive dye acridine orange. Biochim. Biophys. Acta 1110(1): 123-126, 1992. I.F.(1992)=2.610. - Marsigliante S., Verri T., Barker S., Jimenez E., Vinson G.P., Storelli C. Angiotensin II receptor subtypes in eel (Anguilla anguilla). J. Mol. Endocrinol. 12(1): 61-69, 1994. I.F.(1994)=2.232. - Perego C., Markovich D., Norbis F., Verri T., Sorribas V., Murer H. Expression of rat ileal Na+-sulphate cotransport in Xenopus laevis oocytes: functional characterization. Pflügers Arch. - Eur. J. Physiol. ...
Cara Cara oranges are a type of navel orange that has been steadily growing in popularity over the past few years. The oranges are a hybrid between the Washington navel orange and the Brazilian Bahia navel orange, and they were first discovered in 1976 in Venezuela at a place called Hacienda Cara Cara, which is where their name comes from. The Cara Caras look similar to regular navel oranges from the outside, but they have a distinctive pink color to their flesh that is immediately noticeable when you cut into one. The color is similar to that of a ruby red grapefruit, but ranges from a pale pink to a much darker shade from orange to orange. They are sweet, juicy and have a very low acidity. They are also noted for having a more complex flavor than your average orange, with notes of cranberry, grapefruit or even tart cherries in with the orange flavor.. Cara Caras can be used in any recipe that calls for oranges, whether it is in the form of orange juice or orange zest. They are very popular ...
氯乙烯類化合物廣泛應用於乾洗及金屬除油,但因不當處置或意外滲漏,使氯乙烯類化合物污染土壤與地下水體,又因氯乙烯類污染物具高毒性,對環境與民眾健康構成嚴重威脅。基於環境友善與經濟效益,生物復育是目前推崇的整治方法之一,但採用生物復育策略之前,須對污染場址微生物菌相,甚至目標污染物之降解功能性基因與菌株有一定的掌握,以瞭解場址是否具有生物整治潛勢。高氯數氯乙烯類化合物的降解主要以厭氧還原脫氯為主,著名菌屬Dehalococcoides不同菌種具有不同還原脫氯功能性基因(vcrA、bvcA與tceA),本研究針對污染場址的菌屬Dehalococcoides暨其還原脫氯功能性基因進行分析與定量;另使用次世代定序技術分析場址枯、豐水期菌相是否產生差異,特別是還原脫氯及產氫菌群之變化;並使用螢光染劑Acridine orange (AO
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With a vegetable peeler, remove the orange skins (leave the white part intact for now) and cut them into small strips - about ⅛ inch wide and 2 inches long. Simmer the peels in water for 10 to 12 minutes, until tender. Drain them and rinse them in cold water, drying on a paper towel.. Next, cut the white part of the peel off of the oranges so that the orange is exposed. On one end of each orange, cut a small piece off to leave the bottom flat so that the orange can stand up. Arrange them in a serving dish, standing up on their flattened bottoms.. In a saucepan, boil the sugar and water to the firm ball stage. If you have a candy thermometer, this is ideal, and the temperature should be 244 degrees. Once it has reached this temperature, put the blanched orange peels into the syrup and boil for just a minute or two until the syrup has thickened again. Now spoon the syrup over the oranges and chill until serving time.. ...
Ive been on an orange kick lately (I dont normally eat oranges), and I randomly googled oranges. I found this article in the Washington Post a few days ago about a bacterial infection plaguing oranges in Florida, huanglongbing. Im guessing the price of oranges will go up. I was reminiscing recently about the best oranges…
The How to make your very own Orange Man video is below). As I said in my last post….this is the shit that my mind comes up with in the wee hours of the night. Orange Man appeared in my life one day when Shawn sent me a picture of Orange Man hanging out in a coffee shop. Then later sitting on a shelf, and lastly face down on a keyboard tired from a hard day at work (pic up above.). Last week I shared a few on social media. AND then…..I woke up in middle of the night thinking…..hey, ORANGE is the color used for MS - Sooooo Orange Man should SUPPORT MS!!!. And…Orange Mans presence in the MS community began to take root.. FIRST there was his Twitter handle: @OrangeMan4MS. THEN his very own Instagram account: orangeman4ms. ...
NFC, which dominates juice sales and is most associated with all thats pure and simple, is indeed a processed product. Its story involves chemistry, globalization, and questions of land use. Oranges undergo what the industry calls hard extraction (not a gentle squeeze). Extracted juice is vacuum-stripped of oxygen, de-oiled, and held in massive tanks (holding as much as 1.5 million gallons), sometimes for more than a year, before being made palatable via a flavor pack, a proprietary blend of orange essence and orange oil, themselves composed of hundreds of chemicals. (Ethyl butyrate is the one that most pleases North American palates.) The flavor pack is water-soluble, which means that some water goes in with it. But its still Florida orange juice, right? Not really: as Florida growers lose land to real-estate developers, more and more oranges and essence and oil come from Brazil, where land and labor are cheaper and environmental regulation minimal ...
New for HPI Formula Ten cars is this aluminum motor mount. It replaces the stock plastic motor mount with a precision machined aluminum mount for extra rear pod stiffness, extra motor cooling and more durability. The mount is anodized bright orange for a custom look that matches other Formula Ten option parts.. Mounting hardware is included. Check out the full range of performance option parts for the Formula Ten! #61644 SERVO SAVER SET (Cyclone 12) #102877 BALL BEARING SET (FORMULA TEN) #102878 BALL DIFFERENTIAL SET (95 TOOTH/64 PITCH) #102879 THREADED ALUMINUM SHOCK SET #102908 ALUMINUM HEX WHEEL HUB (ORANGE/2pcs) #102909 ALUMINUM FLUORINE COATED FRONT WHEEL SHAFT (2pcs) #102910 ALUMINUM SERVO MOUNT SET (ORANGE) #102911 ALUMINUM MOTOR MOUNT (ORANGE) #102912 ALUMINUM LEFT BULKHEAD (ORANGE) #103018 ALUMINUM REAR AXLE SHAFT 6.3x130mm (ORANGE) #103016 BRIDGESTONE HIGH GRIP FT01 SLICK TIRE M (FRONT) #103017 BRIDGESTONE HIGH GRIP FT01 SLICK TIRE M (REAR) #102906 BRIDGESTONE HIGH GRIP FT01 SLICK TIRE ...
Best price in Australia on American Health Ester C 250 mg - Orange Flavor Wafers, Orange - 125 chewable wafers from eVitamins.com. Find Ester C 250 mg - Orange Flavor Wafers, Orange reviews, side effects, coupons and more from eVitamins. Fast and reliable shipping to the Australia. Ester C 250 mg - Orange Flavor Wafers and other products by American Health for all your health needs.
Current chemotherapies for solid tumors remain severely limited due to toxicity and the development of drug resistance. Hence, new agents are vital to explore in order to improve patient outcomes.. We have identified the novel chelator Dp44mT as a potent and selective anti‐tumor agent that overcomes resistance to standard chemotherapeutics (Whitnall, M., Howard, J., Ponka P. and Richardson, D.R. PNAS USA 2006; 103:14901-6).. To understand its mechanism of action and potential anti‐angiogenic activity, we have investigated its ability to interact with cellular copper (Cu). In contrast to its marked ability to potently mobilize cellular 59Fe, Dp44mT caused marked intracellular 64Cu accumulation. Cellular fractionation demonstrated that 64Cu was concentrated in a fraction containing lysosomes and mitochondria. Moreover, the lysosomotropic character of Dp44mT suggested that it may become concentrated in lysosomes. To test this, studies with the lysosomotropic agent, acridine orange, demonstrated ...
Bullacta exarata was hydrolyzed with trypsin to prepare peptides; Hydrolysates were isolated by ultrafiltration and purified using G-25 gel filtration. The purity of the Bullacta exarata was demonstrated by HPLC and its peptide sequence analysis was detected. The effects of BEPT II and BEPT II-1 on the proliferation of PC-3 cells were examined using a MTT assay. BEPT II and BEPT II-1 significantly inhibited the proliferation of PC-3 cells in a time- and dose-dependent manner. Annexin V/PI double staining studies showed exposing PC-3 cells to 5, or 15 mg/mL BEPT II-1 for 24 h increased the percentage of the early stage of apoptotic cells from 11.22% to 22.09%. In addition, typical morphologic changes were observed in the cells with acridine orange/ethidium bromide staining. These data support that BEPT II-1 has anticancer properties and merits further investigation to understand the mechanisms of BEPT II-1-induced apoptosis in PC-3 cells.
Diabetes was induced in rats by administration of a single i.p. injection of streptozotocin (50 mg/kg body wt). After 7 d, diabetic rats were further treated with insulin or 1,25-dihydroxycholecalciferol [1,25(OH)2D3] for an additional 5-7 d. Control, diabetic, diabetic + insulin, and diabetic + 1,25(OH)2D3 rats were then killed, their proximal small intestines were removed, and villus-tip epithelial cells were isolated and used to prepare brush-border membrane vesicles. Preparations from each of these groups were then analyzed and compared with respect to their amiloride-sensitive, electroneutral Na(+)-H+ exchange activity, using 22Na uptake as well as acridine orange techniques. The results of these experiments demonstrated that (a) H+ gradient-dependent 22Na uptake as well as Na+ gradient-dependent transmembrane H+ fluxes were significantly increased in diabetic vesicles compared to their control counterparts, (b) kinetic studies demonstrated that this enhanced 22Na uptake in diabetes was a ...
Orange discoloration anywhere in the body is not common. For example, there may be a yellowish discoloration of the skin as in jaundice but most of the time we would not expect an orange hue to the skin. It can however occur like with tanning sprays and colorants (fake tans). In fact some of the causes of orange stool may also contribute to a yellow to orange hue of the skin.. Normally stool is a brown color and varies from a tan hue to dark brown in color. This color is due to an accumulation of undigested food, wastes and other substances that are retained in the gut to be expelled with a bowel movement. One of the major contributors of the characteristic brown color of stool is a substance known as stercobilin which is derived from bilirubin (a strong yellow colored pigment produced by the liver).. Bile is stored in the gallbladder and released when food is being digested, especially with the presence of fatty foods, as bile emulsifies fats to help with digestion. If there is any problem ...
Carlson for Kids - The Very Finest Fish Oil Orange encourages healthy brain development and vision in growing children through the use of the 800mg of omega-3s that are in each half-teaspoon. This delicious orange oil is free of vitamins A and D. The ingredients in this product are derived from cold-water fish that are located in deep Norwegian waters. Carlson for Kids - The Very Finest Fish Oil Orange is packaged in Norway so that you get the freshest product possible. This product is tested using AOAC international procedures to promote freshness, effectiveness and purity. An impartial, FDA-registered laboratory examines Carlson for Kids - The Very Finest Fish Oil Orange. Each serving is fresh, strong and does not contain any harmful levels of mercury, cadmium, lead, PCBs or 28 other impurities. Carlson for Kids - The Very Finest Fish Oil Orange is a delicious tasting and convenient way to improve brain development and vision.
Located in Calumet Park, Mr. Orange Chickens fresh chicken is tender and filled with flavor. The whole family can enjoy a meal at this restaurant with its kid-friendly fare.Cant find your khakis? No problem! Throw on a pair of your most comfortable jeans and youll blend right in at Mr. Orange Chicken. You want food. You can take it or well leave it ? just as simple as that. Let us know your preference.Drivers will be happy to know that Mr. Orange Chicken is located near many street and lot parking options.The eats are cheap here. Mr. Orange Chicken knows a meal out should be tasty but not an investment. Flavorful, juicy chicken is hard to come by, and Mr. Orange Chicken is serving it up tens of different ways!
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Orange is the New Black Orange Prisoner Adult Womens Costume - Surviving Litchfield Penitentiary is no easy feat, even if you sport the Officially Licensed Orange is the New Black Orange Prisoner Adult Womens Cos
225-51-4 - OAPPEBNXKAKQGS-UHFFFAOYSA-N - Benz(c)acridine - Similar structures search, synonyms, formulas, resource links, and other chemical information.
Maritz Africa disclaims all liability for any loss, damage, injury or expense however caused, arising from the use of, or reliance upon, in any manner, the information provided through this service and does not warrant the truth, accuracy or completeness of the information provided. The publishers permission is required to reproduce the contents in any form. Copyright © 2020 - Maritz Africa. All Rights Reserved ...
In the same saucepan, mix the water, 1 cup of the sugar and orange juice and cook over medium heat for about a minute, stirring occasionally, until the sugar melts. Add the orange zest and bring to a boil over medium-high heat. Reduce the heat to medium-low and simmer for about 10 minutes. Remove from the heat and let the zest cool in the syrup to room temperature.. Drain the zest and reserve the syrup for another use. Transfer the zest to a small bowl and sprinkle with the remaining tablespoon of sugar. Toss to coat and then let the zest stand at room temperature for about 2 hours to give it time to cool and absorb the sugar. Use right away or store in an airtight container for up to 24 hours.. To make the mousse: Fill a large bowl with ice and water and keep it close at hand.. In a saucepan, sprinkle the gelatin over the cold water and let it sit for about 4 minutes to soften. Add the boiling water and stir until the gelatin dissolves.. Stir in the orange juice, sugar and salt. If the mixture ...
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The newfound source of income is fast replacing orange as the main cash crop. Agriculture: The festive mood is still on after Dasain, but farmer Kiran Rai from Dunglagang, Tsirang is already out in the field tending to his chili seedlings. There is no time to waste as he is trying to beat others to produce the first local chilies.. Not long ago, farmers like Kiran Rai would sit back waiting for middlemen to roam their orange orchards. Orange or citrus mandarin was the main cash crop and farmers turned whatever land they owned into orange orchards.. This is changing.. With most of the trees in warmer places dying or dead, Tsirang farmers are slowly but increasingly turning to vegetables. It is a good and reliable source of income.. Tsirang farmers are as busy as bees.. Everyday is important for Kiran. The first produce fetches the best price, says Kiran. Last year, he produced about 10 quintals of chilli, which fetched him about Nu 70,000. Some farmers earn between Nu 100,000 and 150,000 ...
Hi Jess...again, not a holiday, but we just wrapped up the 2008 Orange Conference in Atlanta on April 30. I have been following your blog for a while just getting ideas because Im the conference director for the Orange Conference. Its hosted by the reThink Group, and were an organization that equips and trains churches about family ministry. Its the whole idea that if red represents the family (the color of blood and warmth) and yellow represents the church (like light), then when these two work together to reach the family, you get a brilliant, beautiful orange. Fun stuff...www.theorangeconference.com. Thanks for a great blog ...
The Stent Placement Kit Orange is stainless steel and contains three parts: Stent Introducer Tube, Stent Plunger and Stent Loader all of which possess the orange identification ring. The orange kit should be used in conjunction with the orange bronchial tube when placing stents 16 -18 mm and all Y-Stents.. Product URL : http://www.bryancorp.com/stent-placement-kit.cfm. Last Updated : 11/09/2012. Categories : Stents, Tracheal ...
Orange Leleupi Cichlid The orange leleupi cichlid, sometimes called the lemon leleupi cichlid, ranges from a yellow to deep orange color. Orange is the most common and preferred color and the color offered by AquariumFishSale.com. Neolamprologus leleupi were the first known Tanganyikans bred for the aquarium. The leleu
I made the smaller of the two wedding cakes in The Book for my graduation party, and this was the frosting that went with the recipe. I love frosting. I particularly love mousseline buttercreams, so I was quite optimistic about this recipe. Much to my dismay though, this frosting was bad. The texture was lovely, as it always is in mousseline buttercream, so it was really the flavor that was the problem. Instead of tasting like orange (as you might have guessed from the name) this frosting tasted only of one thing: butter! Now, I like butter, but this frosting tasted just like whipped butter, which isnt particularly delicious on cake. It was especially frustrating because this recipe was seriously time-consuming. Mousseline buttercream takes a long time, and this one also had orange curd folded in. The orange curd tasted good before it was added to the frosting, but the orange flavor of it got lost behind all the butter in the final product. The cake that this frosting went with was excellent, ...
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Orange facts - 51 Interesting facts about oranges. Did you know... Orange is the worlds favorite flavor after Chocolate and vanilla.
Homemade Orange Chicken made with a sweet & savory orange sauce and tender breaded chicken. This orange chicken recipe with orange marmalade is simple and perfect alongside white rice!
Can PKD patients eat orange and lemon? Kidney patients are very worried about the food they eat. Eating improperly will worsen their condition. So is orange and lemon good for PKD patients? First, lets see the benefits of orange. Orange can prevent k
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The freeMD virtual doctor has found 18 conditions that can cause Coughing Mucus and Pee is Too Orange. There is 1 somewhat common condition that can cause Coughing Mucus and Pee is Too Orange. There are 4 uncommon conditions that can cause Coughing Mucus and Pee is Too Orange. There are 13 rare conditions that can cause Coughing Mucus and Pee is Too Orange.
Detect 4 Drugs (D4D) is an aerosol reagent packaged as a single spray canister, yet enables the user to detect FOUR (4) types of drugs in a single test session! The product is a 100-test size canister that is able to make a preliminary detection and identification of 4 drugs from a single trace collection. Wiping any surface once with the Special Test paper #630, and spraying with D4D will reveal traces of: Hashish or Marijuana, as reddish brown stains; Heroin, as purple gray stains; Amphetamine, as canary-yellow stains; and Meth-amphetamines, as orange stains. D4D is available in kit #1002, consisting of one aerosol canister of DETECT 4 DRUGS, two dispensers of #630 test papers, one aerosol canister of COCA-TEST (for detecting and identifying Cocaine/Crack) and two dispensers of #530 collection papers. This configuration covers most of the common illicit drugs used ...
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As you take a sip of White Claw Hard Seltzer Surge Blood Orange, a potent, unmistakable blood orange taste bursts through, perfectly balanced with the right acidity and a hint of sweetness. Not overpowering, the new flavor is mouth-watering, like a mixed drink muddled with fresh blood orange.
Healthcare trade publication Beckers Infection Control & Clinical Quality has listed Orange Regional Medical Center in Middletown among the top 49 hospitals in the nation with the lowest death rates in the 30-days after hospital care for heart attack. According to data from The Centers for Medicare and Medicaid Services (CMS), Orange Regionals rate of 11.4 percent puts the hospital better than the national average of 14.1 percent. All of the hospitals on the list have a 30-day mortality rate from heart attack of 11.5 percent or less. The figures are from CMS Hospital Compare website and represent data from July 2012 through June 2015, the most recent information available. CMS consider 30-day mortality rates to be an outcome of care measure. The evaluation of an outcome using a standardized period of assessment complies with the standards for such measures as articulated in a scientific statement by the American College of Cardiology and the American Heart Association. Rose Baczewski, Chief Quality
This orange ribbon tac pin has an orange ribbon and represents leukemia. The orange cancer pin makes a wonderful gift for someone going through treatment.
Orange Coast, the magazine of Orange County, showcases monthly coverage of the people, places and events throughout the community. As well, we offer a variety of print and digital products and custom content. Orange Coast - Premiere OC - Design OC - OC Eats - Pasadena ...
With the arrival of winter we begin to put on the table the oranges. Delicious, juicy, oranges enjoy many properties, in particular red oranges than other
Palestine Remembered (فلسطين في الذاكرة)/14193: Jaffa-Jaffa: Before nailing the orange boxes, and after quality control, each orange is carefully wrapped. Matson Collection /14193-Pictures, Stories, Nakba Oral hsitory, Palestine Satellite & Street View, and maps
[vc_row][vc_column][vc_column_text]Critical Orange Punch is a feminized photoperiod seed variety, the first Dutch Passion strain based on outstanding Kritikal Bilbo genetics from Spain. The breeding began by crossing elite cuttings of Grandaddy Purps and Orange Bud (Orange Punch). The result was a supreme smoke with great taste but below-average yields. When this was further crossed with... Read More
... also binds to acridine orange. Chlorophyllin has been validated to exhibit ameliorative effects against food ...
Acridine derivatives: proflavin, acridine orange, acridine yellow, etc. Arylmethine derivatives: auramine, crystal violet, ... DRAQ7 and CyTRAK Orange Pyrene derivatives: cascade blue, etc. Oxazine derivatives: Nile red, Nile blue, cresyl violet, oxazine ...
Darzynkiewicz Z, Kapuscinski J. (1990)"Acridine Orange, a Versatile Probe of Nucleic Acids and Other Cell Constituents." ... Another example of metachromatic dye (fluorochrome) is acridine orange. Under certain conditions it stains single-stranded ...
Hathaway WE, Newby LA, Githens JH (1964). "The Acridine Orange Viability Test Applied to Bone Marrow Cells. I. Correlation with ...
Cell apoptosis is tested by treating the lysosomal membrane with acridine orange. Acridine orange radiates a red fluorescent ...
Traganos F, Darzynkiewicz Z (1994). "Lysosomal proton pump activity: supravital cell staining with acridine orange ...
... consists of two acridine orange subunits that are bridged by a linear oxygenated spacer. Its fluorophore, and ... Ethidium bromide GelRed SYBR Green I Agarose gel electrophoresis and gel electrophoresis of nucleic acids Acridine orange US ... therefore its optical properties, are essentially identical to those of other N-alkylacridinium orange dyes. When exposed to ...
Acridine orange (AO) is a nucleic acid selective fluorescent cationic dye useful for cell cycle determination. It is cell- ... The stain may also be used in conjunction with acridine orange (AO) in viable cell counting. This EB/AO combined stain causes ... In a skillfully made H&E preparation the red blood cells are almost orange, and collagen and cytoplasm (especially muscle) ... It uses a combination of haematoxylin, Orange G, eosin Y, Light Green SF yellowish, and sometimes Bismarck Brown Y. Periodic ...
Acridine orange (AO) staining: Next, 1.20 ml of AO staining solution with 6 µg AO/ml staining buffer is added into the mixture ... Through acridine orange (AO) staining, AO molecules are intercalated into double-stranded DNA in intact sperms while ... Acridine orange test and toluidine blue assay". Andrologia. 49 (10): e12765. doi:10.1111/and.12765. PMID 28224638. S2CID ...
1977). Bacterial cells were counted with an epifluorescence microscope, producing what is called an "acridine orange direct ...
Several dyes have been studied for MUSE's application, including eosin, rhodamine, DAPI, Hoechst, acridine orange, propidium ...
The blood is taken in a QBC capillary tube which is coated with acridine orange (a fluorescent dye) and centrifuged; the ...
The blood is taken in a QBC capillary tube which is coated with acridine orange (a fluorescent dye) and centrifuged; the ...
Fluorescent dyes include DAPI, Acridine Orange, SYBR Green 1, and YO-PRO-1, all of which are capable of staining both DNA and ... Gonzalez K, Mcvey S, Cunnick J, Udovichenko IP, Takemoto DJ (1995). "Acridine orange differential staining of total DNA and RNA ... while Synechococcus emits both orange and red fluorescence; orange from phycobilins and red from chlorophyll. Besides ...
Cooperative Complex Formation of Acridine Orange with poly-l-glutamic Acid] (in German). University of California. OCLC ...
M Garciafernandez; D Ceccarelli; U Muscatello (2004). "Use of the fluorescent dye 10-N-nonyl acridine orange in quantitative ... Jacobson J, Duchen MR, Heales SJ (2002). "Intracellular distribution of the fluorescent dye nonyl acridine orange responds to ... Keij JF, Bell-Prince C, Steinkamp JA (2000). "Staining of mitochondrial membranes with 10-nonyl acridine orange, MitoFluor ... Based on this special structure, the fluorescent mitochondrial indicator, nonyl acridine orange (NAO) was introduced in 1982, ...
Affected cells do not exhibit hallmarks of apoptosis but rather autophagy as seen by staining with acridine orange and ...
B. turicatae is best seen by dark-field microscopy, but the organisms can also be detected using acridine orange or Wright's ...
They applied high alternating voltages in air to materials such as acridine orange dye, either deposited on or dissolved in ...
... as revealed by acridine orange staining and PCR-based diagnoses". Tropical Medicine and International Health. 3 (4): 304-312. ...
... acridine orange stain, and phase-contrast microscopy. It is capable of forming biofilms and can convert from spiral to a ...
An exception is the metachromatic fluorochrome acridine orange, which under the specific staining protocol can differentially ... or DNA susceptibility to denaturation at low pH using the metachromatic dye acridine orange, reveals the G1Q, G1A, and G1B cell ... Aside from propidium iodide and acridine orange, quantifiable dyes that are frequently used include (but are not limited to) ...
... when Bernanose and coworkers first produced electroluminescence in crystalline thin films of acridine orange and quinacrine. In ...
... include acridine orange, methylene blue, curcumin, and indocyanine green. A study by Suzuki et al. used acridine orange, a ...
... acridine orange MeSH D03.494.046.250.177 - acriflavine MeSH D03.494.046.250.200 - aminacrine MeSH D03.494.046.250.225 - ...
Psoralens, coal tars, photo-active dyes (eosin, acridine orange) Musk ambrette, methylcoumarin, lemon oil (may be present in ... In these areas an unsightly orange to brown tint may form, usually near or on the face. Many medications and conditions can ...
The flow-cytometric assay utilizing the fluorochrome acridine orange shows that DNA fragmentation within individual cells is ...
Smears of the kidney, liver, and spleen can be stained with Gram, Giemsa, acridine orange, or methylene blue stain for direct ...
The molecular formula C17H19N3 (molar mass : 265.35 g/mol) may refer to: Acridine orange Antazoline Esmirtazapine Mirtazapine A ...
... and then in 1985 of the F plasmid by acridine orange curing.[citation needed] Strains derived from MG1655 include DH1, parent ...
It has the form of an orange or brown powder. It may be harmful in the eyes or if inhaled. It is a dye and it stains the skin ... It is derived from acridine. Commercial preparations are often mixtures with proflavine. It is known by a variety of commercial ...
... damages DNA and is used as a mutagen in microbiology. Acridine yellow is similar to acridine orange. According ... Acridine yellow, also known as acridine yellow G, acridine yellow H107, basic yellow K, and 3,6-diamino-2,7-dimethylacridine, ... It is a derivate of acridine. In histology, it is used as a fluorescent stain, and as a fluorescent probe for non-invasive ... Acridine yellow absorption and emission spectra v t e (Articles needing additional references from July 2021, All articles ...
Acridine derivatives are also G4 ligands and through fluorescence resonance energy transfer (FRET) melting assays, ... The ligands for i-motif used as fluorescent probes include Thiazole orange, 2,2'-diethyl-9-methylselenacarbocyanine bromide ( ... Tilorone and Tobramycin are i-motif binding ligands discovered via thiazole orange fluorescence intensity displacement (FID) ... diethylenetriamine (BisA) was determined to increase the melting temperature of both i-motif and G4, while monomeric acridine ( ...
... and related derivatives (such as amsacrine) bind to DNA and RNA due to their abilities to intercalate. Acridine orange ... Acridine is easily oxidized by peroxymonosulfuric acid to the acridine amine oxide. The carbon 9-position of acridine is ... at one time acridine dyes were popular, but they are now relegated to niche applications, such as with acridine orange. The ... biologically active acridines, applications of acridines, new syntheses and reactions of acridines] Wikisource has original ...
Its reaction with benzenediazonium chloride gives the cationic azo dye Basic Orange 1. Condensation of 2,4-diaminotoluene with ... acetaldehyde gives the acridine dye called Basic Yellow 9. NIOSH Pocket Guide to Chemical Hazards. "#0620". National Institute ...
When exposed to ultraviolet light, it will fluoresce with an orange colour, intensifying almost 20-fold after binding to DNA. ... Its core heterocyclic moiety is generically known as a phenanthridine, an isomer of which is the fluorescent dye acridine. ... As a result of this excitation, EtBr emits orange light with wavelength 605 nm. Ethidium bromide's intense fluorescence after ...
... and fluorescein have a maximum excitation at 502nm and 525 nm (green). When acridine orange associates with RNA ... Acridine orange is useful in the rapid screening of ordinarily sterile specimens. When acridine orange is used with flow ... When the pH of the environment is 3.5, acridine orange becomes excited by blue light (460 nm). When acridine orange is excited ... using acridine orange is a method known for examining the microbial content within food and water. The use of acridine orange ...
... acridine Dibenz[c,h]acridine 7H-Dibenzo[c,g]carbazole Dibenzo[a,e]pyrene Dibenzo[a,h]pyrene Dibenzo[a,i]pyrene Dibromoacetic ... Nitrosomethylvinylamine N-Nitrosomorpholine N-Nitrosopiperidine N-Nitrosopyrrolidine N-Nitrososarcosine Ochratoxin A Oil Orange ...
Acridine has been obtained as eight polymorphs and aripiprazole has nine. The record for the largest number of well- ... Krämer, Katrina (2020-07-29). "Red-orange-yellow reclaims polymorph record with help from molecular cousin". chemistryworld.com ... Acridine Solid Form Landscape: Eight Polymorphs and a Hydrate" (PDF). Crystal Growth & Design. 19 (8): 4884-4893. doi:10.1021/ ...
Fluorescent microscopy and the identification of malaria parasites using an acridine-orange staining technique / by G. T. Shute ...
Acridine orange and fluorescein have a maximum excitation at 502nm and 525 nm (green). When acridine orange associates with RNA ... Acridine orange is useful in the rapid screening of ordinarily sterile specimens. When acridine orange is used with flow ... When the pH of the environment is 3.5, acridine orange becomes excited by blue light (460 nm). When acridine orange is excited ... using acridine orange is a method known for examining the microbial content within food and water. The use of acridine orange ...
Acridine orange stained blood wet mounts for fluorescent detection of malaria.. Authors: Hemvani, N. Chitnis, D S. Dixit, D S. ... The acridine orange stained blood wet mounts over 2420 suspected malaria cases from Indore city were examined under fluorescent ... The modified acridine orange stained method is simple, instant and more efficient, requires less scanning time and skill, ... Acridine orange stained blood wet mounts for fluorescent detection of malaria. Indian Journal of Pathology & Microbiology. 1999 ...
Acetic acid + acridine orange. M-CM. Basal cell carcinoma23 Acridine orange + ethidium bromide. 3-colour FCM. Basal cell ... Acridine orange, methylene blue, toluidine blue, fluorescein, Nile blue or Patent Blue V. FCM. Skin tumours22 ... AO, acridine orange; BCC, basal cell carcinoma; FCM, fluorescence confocal microscopy; NPV, negative predictive value; PPV, ...
By acridine orange staining. ,,,Click on the PDF icon to the left to view a copy of this virus entry in PDF format. You can get ...
Acridine Orange [‎1]‎. Acridines [‎1]‎. Acrolein [‎3]‎. Acrylamide [‎5]‎. Acrylamides [‎5]‎. Acrylates [‎2]‎. ...
Use of the fluorescent dye 10-N-nonyl acridine orange in quantitative and location assays of cardiolipin: a study on different ... Use of the fluorescent dye 10-N-nonyl acridine orange in quantitative and location assays of cardiolipin: a study on different ... Use of the fluorescent dye 10-N-nonyl acridine orange in quantitative and location assays of cardiolipin: a study on different ... The fluorescent dye 10-N-nonyl acridine orange (NAO) is extensively used for location and quantitative assays of cardiolipin in ...
Any granulomatous lesions were then stained with a modified Ziehl-Neelson procedure and an auramine orange and acridine orange ... Auramine O and acridine orange staining procedure. In: Standard operating procedure. Ames (IA): Center for Veterinary Biologics ...
Manufacturer, supplier, Trader and wholesaler of Acridine Orange Zncl2 offered by ARNISH LABORATES PRIVATE LIMITED. at latest ... ACRIDINE ORANGE ZNCL2. As one of the trusted manufacturer, exporter and supplier of allied domain, we have been engaged in ... Moreover, our clients can purchase this ACRIDINE ORANGE ZNCL2 negotiable prices from us. ... providing our clients with excellent quality ACRIDINE ORANGE ZNCL2. This chemical is widely used in processing of xylenlol ...
So, While Im playing the game the game with crash to desktop, no warning or notification of the crash the game will just close and I have to relaunch the game. I am running the game through the Epic Games Store. My specs are: CPU: Ryzen 7 1700X OCed to 3.8Ghz GPU: 2x RX 580 8Gbs OS: Windows 10 P...
Detection of Bartonella (Rochalimaea) quintana by routine acridine orange staining of broth blood cultures. J Clin Micro. June/ ...
Acridine orange zinc chloride double salt (C.I.46005) for microscopy Certistain® - Find MSDS or SDS, a COA, data sheets and ... Acridine orange zinc chloride double salt (C.I.46005) for microscopy Certistain® ... 1159310025 Sigma-AldrichAcridine orange zinc chloride double salt (C.I.46005) for microscopy Certistain®. ...
Acridine Orange direct count (AODC). Shaking and addition of formalin and refrigerate. Baltic Sea 1974/92. ... Acridine Orange direct count (AODC). Shaking and addition of formalin and refrigerate. Baltic Sea 1974/92. ...
Total count was determined using an acridine orange staining method followed by epifluorescence microscopic counting. The ...
The differences in levels of DNA fragmentation were determined with acridine orange test, which was conducted before and after ... study the effect of PAPA nutritional supplement on the levels of DNA fragmentation of sperm cells tested with acridine orange ... orange and red). After treatment the level of sperm DNA fragmentation decreased by 10.2%. Conclusion: Men with confirmed ...
AO: Acridine orange, PI: propidium iodide, EA: early apoptotic cells, LA: past due apoptotic cells, N: necrotic cells, H: ... AO: Acridine orange, PI: propidium iodide, EA: early apoptotic cells, LA: past due apoptotic cells, N: necrotic cells, H: ...
Optimization of acridine orange loading on 1:1 layered clay minerals for fluorescence enhancement. / Jiang, Wei Teh; Tsai, ... Optimization of acridine orange loading on 1:1 layered clay minerals for fluorescence enhancement. 於: Journal of Industrial and ... 深入研究「Optimization of acridine orange loading on 1:1 layered clay minerals for fluorescence enhancement」主題。共同形成了獨特的指紋。 ... Jiang, W. T., Tsai, Y., Wang, X., & Li, Z. (2020). Optimization of acridine orange loading on 1:1 layered clay minerals for ...
MitoTracker Orange/MeOH, Cy3™, Rhod-2, Alexa Fluor 555™, tdTomato, DyLight 549, Atto 550, TAMRA, Tetramethylrhodamine ... Acridine Orange + DNA, Atto 488, BCECF/pH 9.0, LysoTracker Green/pH 5.2, Fluo-3, BODIPY FL/pH7.2, Propidium Iodide, Quasar® 570 ... 39009 - AT - Acridine Orange/Di-8-ANEPPS. 39010 - AT - Texas Red/mCherry/AlexaFluor 594. ...
AO, acridine orange; APO, aposymbiotic; WT, clarinex order online wild type. Kewalo Marine Laboratory. ...
Acridine Orange (+DNA). 502. 526. Acridine Yellow. 470. 550. Alexa Fluor® 430. 431. 541. ® 430>. ...
Acridine orange (AO) and ethidium bromide (EB) staining. HUVECs were added into 24-well plates, grown to 80% confluence, and ... B) Acridine orange/ethidium bromide staining of human umbilical vein endothelial cells; magnification, ×20. The ED level of ...
AO, acridine orange; APO, aposymbiotic; HCR, hybridization chain what does womens viagra do reaction-fluorescent in situ ...
2011: Mechanism of acridine orange removal from water by low-charge swelling clays, Chem. Engineer. J. (603-611 pp.) ... 2022: Sorption of acridine orange on non-swelling and swelling clay minerals, Crystals (118 pp.) ... 2016: Structure and fluorescent properties of Ba3Sc(PO4)3: Sm3+red-orange phosphor for n-UV w-LEDs, Chem. Phys. Lett. (212-215 ... 2016: Ionic-liquid-crafted zeolite for the removal of anionic dye methyl orange, J. Taiwan Institute Chem. Engineer. (237-243 ...
39009 - AT - Acridine Orange/Di-8-ANEPPS. 39010 - AT - Texas Red/mCherry/AlexaFluor 594. ...
Acridine Orange (+DNA). 502. 526. Alexa Fluor® 430. 431. 541. ® 430>. Alexa Fluor® 488. 499. 520. ® 488>. ...
However, using the Smithwick method, which employs acridine orange as the specific dye, the M. tuberculosis bacteria glow ... processed using a fluorescence acid-fast staining method that implements acridine orange fluorescent dye as a counterstain, ...
  • Apoptosis was evaluated by acridine orange/ethidium bromide double staining method. (scirp.org)
  • The real-time reversal of tumor MDR were evaluated by flow cytometer, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, acridine orange/ethidium bromide staining, and Western blot analysis. (dovepress.com)
  • Moreover, the pharmacophore modelling evidenced superimposition between linear furanocoumarins, ethidium bromide and acridine orange, thereby suggesting that these compounds share similar biological effects, supported by their acknowledged DNA intercalating activities. (pathofscience.org)
  • Generally in most delicate tumor cells (A549), scorpion venom induced necrosis evidenced by acridine orange/ethidium bromide EC1454 fluorescent down-expression and dyes of apoptosis-related genes. (antiviralbiologic.com)
  • As a cationic fluorescence dye acridine orange (AO) is commonly used in biology and biochemistry for DNA analyses. (ncku.edu.tw)
  • TUNEL assay and acridine orange fluorescence staining were carried out to investigate the molecular mechanismsresponsible for the cell death. (ias.ac.in)
  • Under a magnification of 1000X, this photomicrograph of a sputum smear, processed using a fluorescence acid-fast staining method that implements acridine orange fluorescent dye as a counterstain, revealed the presence of a Mycobacterium tuberculosis bacterium. (cdc.gov)
  • Acridine orange using both epi-fluorescence microscope and flow cytometry. (slu.se)
  • SUMMARY: The staining of viral nucleic acids with acridine orange and their subsequent examination under the fluorescence microscope permits the determination of the type and strandedness. (microbiologyresearch.org)
  • In this paper, we report simultaneous measurements of nuclear area and nuclear fluorescence intensity on individual cells stained with the intercalating dye, acridine orange (AO). (illinois.edu)
  • The Ca2+-induced H+ release visualized by fluorescence quenching of acridine orange correlates well with the Ca2+ uptake determined isotopically. (uc.pt)
  • Fluorescence microscopy of cells stained with acridine orange and propidium iodide verified that medications had little influence RAPT1 on cell viability. (researchreport-digital.com)
  • When acridine orange is excited by blue light, the fluorescent dye can differentially stain human cells green and prokaryotic cells orange (600 nm), allowing for rapid detection with a fluorescent microscope. (wikipedia.org)
  • Past and present studies comparing acridine orange staining with blind subcultures for the detection of positive blood cultures showed that the acridine orange is a simple, inexpensive, rapid staining procedure that appears to be more sensitive than the Gram stain for detecting microorganisms in cerebrospinal fluid and other clinical and non-clinical materials. (wikipedia.org)
  • When acridine orange is used with flow cytometry, the differential stain is used to measure DNA denaturation and the cellular content of DNA versus RNA in individual cells, or detect DNA damage in infertile sperm cells. (wikipedia.org)
  • Acridine orange staining has to be performed at an acidic pH to obtain the differential staining, which allows bacterial cells to stain orange and tissue components to stain yellow or green. (wikipedia.org)
  • Hence, the present study was aimed to improve malarial parasite detection by using acridine orange to stain large blood drops in the form of wet coverglass mounts. (who.int)
  • This study showed that acridine orange stain is more efficient in demonstrating H. felis organisms than the Romanowsky stains and that the May-Grunwald-Giemsa staining method is superior to the other Romanowsky stains in demonstrating H. felis organisms. (gla.ac.uk)
  • Confocal optical section of a living chlorenchyma cell (stained with the nucleic acid stain acridine orange) of Bienertia cycloptera , which possesses a novel mechanism for C 4 photosynthesis. (botany.org)
  • You take a stain, like acridine orange or a fluorescent stain or Giemsa , and a microscope, and you look at them. (singjupost.com)
  • Acridine dyes are prepared via the condensation of 1,3-diaminobenzene with suitable benzaldehydes. (wikipedia.org)
  • Solution 18 contains two different dyes: acridine orange, which stains the entire population of cells, and fluorescent DAPI, which stains non-viable cells, respectively. (aquaanalytics-tekhnika.ru)
  • In this the specimens stained with fluorescent dyes like Auramine, Acridine orange etc. are exposed to the light of shorter wavelength - UV light or X-rays - results in the emission of longer wavelength visible light. (paramedicsworld.com)
  • To time, live image resolution of irritation provides been restricted to experimental animal models, with examples such as magnetic particles (iron oxide and gadolinium chelates) for magnetic resonance imaging, fluorescent nanoparticles which can be tagged with aptamers or peptides targeted against cell surface biomarkers, circulating factors or nucleic acid structures, or dyes such as acridine orange, which is usually a known human carcinogen (Joussen et al. (uberbrain.net)
  • Acridine orange is an organic compound that serves as a nucleic acid-selective fluorescent dye with cationic properties useful for cell cycle determination. (wikipedia.org)
  • The maximum excitation and emission value that occur when acridine orange is bound to RNA are the result of electrostatic interactions and the intercalation between the acridine molecule and nucleic acid-base pairs present within RNA and DNA. (wikipedia.org)
  • Bodipy ceramide labeling (to assay neuroepithelial cell shapes) and acridine orange labeling (to assay cell death) of explants and embryos were performed essentially as described (Brand et al. (techfromastranger.com)
  • IBMX (600 μM) was shown to induce fragmentation in 661W cells stained with 10-N-nonyl acridine orange (NAO), an acridine orange derivative that marks the inner mitochondrial membrane in whole cells. (molvis.org)
  • Ram´s sperm DNA integrity was assessed by SCD (Sperm Chromatine Dispersion) test to study DNA fragmentation and Acridine orange staining was used to estimate DNA denaturation (double-strand DNA versus single-strand DNA). (ac.ir)
  • Acridine orange is able to withstand low pH environments, allowing the fluorescent dye to penetrate acidic organelles such as lysosomes and phagolysosomes that are membrane-bound organelles essential for acid hydrolysis or for producing products of phagocytosis of apoptotic cells. (wikipedia.org)
  • When acridine orange associates with RNA, the fluorescent dye experiences a maximum excitation shift from 525 nm (green) to 460 nm (blue). (wikipedia.org)
  • When acridine orange binds to DNA, the dye exhibits a maximum excitation at 502 nm producing a maximum emission of 525 nm. (wikipedia.org)
  • Nonyl Acridine Orange (NAO) is another potential-independent mitochondrial dye that binds specifically to cardiolipin in the inner mitochondrial membrane. (biotium.com)
  • The dye Acridine Orange then binds to the double stranded DNA (dsDNA) and single stranded DNA (ssDNA) and fluoresces green or red respectively. (andrologycenter.in)
  • The ability to penetrate the cell membranes of acidic organelles and cationic properties of acridine orange allows the dye to differentiate between various types of cells (i.e., bacterial cells and white blood cells). (wikipedia.org)
  • Morphological changes of SGNs were observed by acridine orange cytochemistry staining, and apoptosis was examined by flow cytometry. (nih.gov)
  • Treatment of SGNs with ONOO(-) resulted in the occurrence of caspase-independent apoptosis as evidenced by acridine orange staining and flow cytometry analysis. (nih.gov)
  • Concerning the phenotypic apoptotic changes that were analyzed by Acridine orange and eosin and hematoxylin, Ag NPs caused the apoptosis and Vacuole degeneration as well as cell formation and the emergence of Necrotic cells in MCF-7 cells, whereas in the normal cell line Vero, no change appears in its phenotype. (springeropen.com)
  • Acridine orange is used in epifluorescence microscopy and flow cytometry. (wikipedia.org)
  • Acridine orange has been widely accepted and used in many different areas, such as epifluorescence microscopy, and the assessment of sperm chromatin quality. (wikipedia.org)
  • Acridine has antimicrobial factors useful in drug-resistant bacteria and isolating bacteria in various environments. (wikipedia.org)
  • Acridine orange in the mid-twentieth century was used to examine the microbial content found in soil and direct counts of aquatic bacteria. (wikipedia.org)
  • Additionally, the method of acridine orange direct count (AODC) proved useful in the enumeration of bacteria found within landfills. (wikipedia.org)
  • The use of acridine orange in clinical applications has become widely accepted, mainly focusing on highlighting bacteria in blood cultures. (wikipedia.org)
  • However, using the Smithwick method, which employs acridine orange as the specific dye, the M. tuberculosis bacteria glow yellow under ultraviolet light microscopy. (cdc.gov)
  • Control experiments (obtained by acridine orange direct counting) showed that less than 1% of the bacteria passed through the GF/F filters. (harvard.edu)
  • This discrepancy is particularly dramatic in some aquatic environments, in which plate counts and viable cells estimated by acridine orange staining can differ by four to six orders of magnitude (66), and in soil, in which 0.1 to 1% of bacteria are readily culturable on common media under standard conditions (138, 139). (blogspot.com)
  • The density of sperm during such events can be determined by directly counting numbers of spermatozoa using a modification of the acridine orange direct count (AODC) technique for enumerating bacteria. (nova.edu)
  • When bound to DNA, acridine orange is very similar spectrally to an organic compound known as fluorescein. (wikipedia.org)
  • Acridine orange and fluorescein have a maximum excitation at 502nm and 525 nm (green). (wikipedia.org)
  • Acridine orange is recommended for the use of fluorescent microscopic detection of microorganisms in smears prepared from clinical and non-clinical materials. (wikipedia.org)
  • IMSEAR at SEARO: Acridine orange stained blood wet mounts for fluorescent detection of malaria. (who.int)
  • Hemvani N, Chitnis DS, Dixit DS, Asolkar MV. Acridine orange stained blood wet mounts for fluorescent detection of malaria. (who.int)
  • The commercial 'QBC' like acridine orange stained capillary tube preparation has a limitation of precise species identification and the detection of extra-erythrocytic parasites. (who.int)
  • Autophagy detection using acridine orange staining Acridine orange staining was used to visualize the volume of the cellular acidic compartment (11). (foodmicro2008.org)
  • The differential staining capability of acridine orange provides quick scanning of specimen smears at lower magnifications of 400x compared to Gram stains that operate at 1000x magnification. (wikipedia.org)
  • The acridine orange stained blood wet mounts over 2420 suspected malaria cases from Indore city were examined under fluorescent microscope and the results compared with the Leishman's stained thick blood smears in a blind study. (who.int)
  • The positivity of malarial parasites reported by the modified acridine orange staining was 248 against 109 by Leishman's stained thick blood smears. (who.int)
  • Because of this, we looked into its influence on lysosomes using the lysosomotropic fluorochrome, acridine Rabbit Polyclonal to GTPBP2 orange (AO) as well as the acidophilic dye, LysoTracker Crimson (LTR). (gonzalezgass.com)
  • Acridine orange is cell-permeable, which allows the dye to interact with DNA by intercalation, or RNA via electrostatic attractions. (wikipedia.org)
  • The results presented (paper-'Study-behavior of HnT with DNA intercalator Acridine Orange') conclude that the degree of intercalation can be reduced in the presence of the HNT setting. (cecon.com)
  • Evaluation of acridine-orange staining of centrifuged parasites in malarial infection. (elsevier.com)
  • Acridine orange is derived from the organic molecule acridine, which was first discovered by Carl Grabe and Heinrich Caro, who isolated acridine by boiling coal in Germany during the late nineteenth century. (wikipedia.org)
  • Insets: Acridine orange stains of samples at two distances from the source. (lbl.gov)
  • Acridine orange is useful in the rapid screening of ordinarily sterile specimens. (wikipedia.org)
  • Nondenaturing gels stained with acridine orange, after incubation in ADP, reveal that the activity producing the poly(A) coelectrophoreses with authentic polynucleotide phosphorylase. (uthscsa.edu)
  • RESULTS: The results showed that siramesine killed standard glioma cell lines in vitro, and loss of acridine orange staining suggested a compromised lysosomal membrane. (refbase.net)
  • Lysosomal membrane integrity, evaluated by acridine orange (AO) staining, was impaired by 40 µM SMA-tDodSNO (P<0.05 vs control) and when combined with SMA-Dox, this effect was significantly potentiated (P<0.001 vs SMA-Dox). (unict.it)
  • At the appropriate time points following ApoG2 treatment, the cells were incubated with tradition medium comprising 1 mg/ml acridine orange for 15 min. (foodmicro2008.org)
  • The cells were then stained with acridine orange for 20 min. (foodmicro2008.org)
  • Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. (wikidata.org)
  • Resultaten från Halomax® jämfördes med resultaten från mätningar med epi-fluorescens mikroskop och flödescytometri, i båda fallen färgade med akridin orange. (slu.se)
  • Direct epifluorescent filter technique (DEFT) using acridine orange is a method known for examining the microbial content within food and water. (wikipedia.org)
  • The modified acridine orange stained method is simple, instant and more efficient, requires less scanning time and skill, allows scanning of larger blood volume (75 ul) at lower magnification and the morphological details at higher magnification helps to make the precise species identification. (who.int)
  • Total count was determined using an acridine orange staining method followed by epifluorescence microscopic counting. (cdc.gov)
  • Acridine orange-labeled tissue was imaged using epifluorescence on the BX60 microscope and bodipy ceramide-labeled tissue was imaged on an Olympus IX70 microscope equipped with a BioRad Radiance 2000 confocal laser system. (techfromastranger.com)
  • 2022: Sorption of acridine orange on non-swelling and swelling clay minerals, Crystals (118 pp. (uwp.edu)
  • This chemical is widely used in processing of xylenlol orange, redox indicator and anti-oxidents. (arnishlaborates.in)
  • Metode Total Plate Count digunakan untuk menentukan kandungan bakteri heterotrofik, sedangkan produktivitas bakteri ditentukan dengan metode Acridine Orange Direct Count. (undip.ac.id)
  • Systematic colocalization errors between acridine orange and EGFP in astrocyte vesicular organelles. (mpg.de)
  • The acridine orange was eliminated and fluorescent micrographs were captured using a DM-IRB inverted fluorescent microscope (Leica, Wetzlar, Germany). (foodmicro2008.org)
  • Carried out a study to understand the conduct of HNT with the DNA intercalator Acridine Orange. (cecon.com)
  • Designed to accommodate the growing demand for eco-tourists visiting Hong Kong, all green rooms are equipped with a set of Tarocco skin care and hair care products, a natural range of products rich in Vitamin C and Antioxidants with the presence of Italian olive oil and extracts of Sicilian blood oranges. (malaysianfoodie.com)
  • E , F ) Acridine Orange-stained 2 dpf wild-type (E) and gle1 -/- (F) embryos. (zfin.org)
  • As one of the trusted manufacturer, exporter and supplier of allied domain, we have been engaged in providing our clients with excellent quality ACRIDINE ORANGE ZNCL2 . (arnishlaborates.in)
  • Results: A strong cytolytic effect of the compound was signified by lipid release and acridine orange staining of dying adipocyte nuclei. (mdc-berlin.de)