A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.
A plant species of the genus CITRUS, family RUTACEAE that provides the familiar orange fruit which is also a source of orange oil.
Acridines which are substituted in any position by one or more amino groups or substituted amino groups.
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide.
Agents that are capable of inserting themselves between the successive bases in DNA, thus kinking, uncoiling or otherwise deforming it and therefore preventing its proper functioning. They are used in the study of DNA.
PHENOTHIAZINES with an amino group at the 3-position that are green crystals or powder. They are used as biological stains.
Plasmids which determine the ability of a bacterium to ferment lactose.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
A dye that is a mixture of violet rosanilinis with antibacterial, antifungal, and anthelmintic properties.
A plant genus of the family RUTACEAE. They bear the familiar citrus fruits including oranges, grapefruit, lemons, and limes. There are many hybrids which makes the nomenclature confusing.
Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.
Acidic phospholipids composed of two molecules of phosphatidic acid covalently linked to a molecule of glycerol. They occur primarily in mitochondrial inner membranes and in bacterial plasma membranes. They are the main antigenic components of the Wassermann-type antigen that is used in nontreponemal SYPHILIS SERODIAGNOSIS.
A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
An aniline dye used as a disinfectant and an antiseptic agent. It is weakly fluorescing and binds specifically to certain proteins.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A highly fluorescent anti-infective dye used clinically as a topical antiseptic and experimentally as a mutagen, due to its interaction with DNA. It is also used as an intracellular pH indicator.
A neoplasm containing HISTIOCYTES. Important forms include BENIGN FIBROUS HISTIOCYTOMA; and MALIGNANT FIBROUS HISTIOCYTOMA.
Measurement of light given off by fluorescein in order to assess the integrity of various ocular barriers. The method is used to investigate the blood-aqueous barrier, blood-retinal barrier, aqueous flow measurements, corneal endothelial permeability, and tear flow dynamics.
Inorganic compounds that contain zinc as an integral part of the molecule.
Inorganic compounds derived from hydrochloric acid that contain the Cl- ion.
A chlorinated bisphenol antiseptic with a bacteriostatic action against Gram-positive organisms, but much less effective against Gram-negative organisms. It is mainly used in soaps and creams and is an ingredient of various preparations used for skin disorders. (From Martindale, The Extra Pharmacopoeia, 30th ed, p797)
A metallic element of atomic number 30 and atomic weight 65.38. It is a necessary trace element in the diet, forming an essential part of many enzymes, and playing an important role in protein synthesis and in cell division. Zinc deficiency is associated with ANEMIA, short stature, HYPOGONADISM, impaired WOUND HEALING, and geophagia. It is known by the symbol Zn.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Chemical agents that increase the rate of genetic mutation by interfering with the function of nucleic acids. A clastogen is a specific mutagen that causes breaks in chromosomes.
A federal area located between Maryland and Virginia on the Potomac river; it is coextensive with Washington, D.C., which is the capital of the United States.
Small computers that lack the speed, memory capacity, and instructional capability of the full-size computer but usually retain its programmable flexibility. They are larger, faster, and more flexible, powerful, and expensive than microcomputers.
A republic in the north of South America, east of VENEZUELA and west of SURINAME. Its capital is Georgetown.
A republic of southeast Asia, northwest of Thailand, long familiar as Burma. Its capital is Yangon, formerly Rangoon. Inhabited by people of Mongolian stock and probably of Tibetan origin, by the 3d century A.D. it was settled by Hindus. The modern Burmese state was founded in the 18th century but was in conflict with the British during the 19th century. Made a crown colony of Great Britain in 1937, it was granted independence in 1947. In 1989 it became Myanmar. The name comes from myanma, meaning the strong, as applied to the Burmese people themselves. (From Webster's New Geographical Dictionary, 1988, p192 & Room, Brewer's Dictionary of Names, 1992, p367)
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Substances produced from the reaction between acids and bases; compounds consisting of a metal (positive) and nonmetal (negative) radical. (Grant & Hackh's Chemical Dictionary, 5th ed)
A tough transparent membrane surrounding the OVUM. It is penetrated by the sperm during FERTILIZATION.
A natural high-viscosity mucopolysaccharide with alternating beta (1-3) glucuronide and beta (1-4) glucosaminidic bonds. It is found in the UMBILICAL CORD, in VITREOUS BODY and in SYNOVIAL FLUID. A high urinary level is found in PROGERIA.
Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.
Interactive processes between the oocyte (OVUM) and the sperm (SPERMATOZOA) including sperm adhesion, ACROSOME REACTION, sperm penetration of the ZONA PELLUCIDA, and events leading to FERTILIZATION.
The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.
A colloidal, hydrated aluminum silicate that swells 12 times its dry size when added to water.
Any of the numerous types of clay which contain varying proportions of Al2O3 and SiO2. They are made synthetically by heating aluminum fluoride at 1000-2000 degrees C with silica and water vapor. (From Hawley's Condensed Chemical Dictionary, 11th ed)
The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.
The study of the energy of electrons ejected from matter by the photoelectric effect, i.e., as a direct result of absorption of energy from electromagnetic radiation. As the energies of the electrons are characteristic of a specific element, the measurement of the energy of these electrons is a technique used to determine the chemical composition of surfaces.
The most common mineral of a group of hydrated aluminum silicates, approximately H2Al2Si2O8-H2O. It is prepared for pharmaceutical and medicinal purposes by levigating with water to remove sand, etc. (From Merck Index, 11th ed) The name is derived from Kao-ling (Chinese: "high ridge"), the original site. (From Grant & Hackh's Chemical Dictionary, 5th ed)
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
Hard, amorphous, brittle, inorganic, usually transparent, polymerous silicate of basic oxides, usually potassium or sodium. It is used in the form of hard sheets, vessels, tubing, fibers, ceramics, beads, etc.
Agents employed in the preparation of histologic or pathologic specimens for the purpose of maintaining the existing form and structure of all of the constituent elements. Great numbers of different agents are used; some are also decalcifying and hardening agents. They must quickly kill and coagulate living tissue.
The technique of using a microtome to cut thin or ultrathin sections of tissues embedded in a supporting substance. The microtome is an instrument that hold a steel, glass or diamond knife in clamps at an angle to the blocks of prepared tissues, which it cuts in sections of equal thickness.
A plastic substance deposited by insects or obtained from plants. Waxes are esters of various fatty acids with higher, usually monohydric alcohols. The wax of pharmacy is principally yellow wax (beeswax), the material of which honeycomb is made. It consists chiefly of cerotic acid and myricin and is used in making ointments, cerates, etc. (Dorland, 27th ed)
A highly reactive aldehyde gas formed by oxidation or incomplete combustion of hydrocarbons. In solution, it has a wide range of uses: in the manufacture of resins and textiles, as a disinfectant, and as a laboratory fixative or preservative. Formaldehyde solution (formalin) is considered a hazardous compound, and its vapor toxic. (From Reynolds, Martindale The Extra Pharmacopoeia, 30th ed, p717)

Comparison of five methods of malaria detection in the outpatient setting. (1/413)

In eastern Africa where 90% of the malaria is due to Plasmodium falciparum, the accuracy of malaria diagnosis at the outpatient level is becoming increasingly important due to problems of drug resistance and use of alternative, costly antimalarial drugs. The quantitative buffy coat (QBC) technique, acridine orange staining with an interference filter system, and the ParaSight-F test have been introduced as alternative methods to conventional microscopy for the diagnosis of malaria. Two hundred thirteen outpatients were tested using these alternative methods and conventional microscopy by five experienced technologists; two were randomly allocated to read the results of each test. Paired results showed the highest level of agreement with the ParaSight-F test (99%), followed by Field stain (92%). The results of the QBC technique showed the least agreement (73%). Using conventional microscopy as the reference standard, the ParaSight-F test had a sensitivity range of 90-92% and a specificity of 99%, staining with acridine orange had a sensitivity range of 77-96% and a specificity range of 81-98% and the QBC technique had a sensitivity range of 88-98% and a specificity range of 58-90%. All microscopic tests showed lower sensitivities (as low as 20% using staining with acridine orange) in detecting low parasitemias (< or = 320/microl) than the ParaSight-F test (70%). Due to the high cost of the ParaSight-F test, Field-stained blood films remain the most appropriate method for diagnosis of P. falciparum in eastern Africa. The ParaSight-F test may be used in situations where no trained microscopists are available, or where malaria is strongly suspected and the results of microscopy are negative.  (+info)

Destruction of protamine in human sperm inhibits sperm binding and penetration in the zona-free hamster penetration test but increases sperm head decondensation and male pronuclear formation in the hamster-ICSI assay. (2/413)

PURPOSE: Our purpose was to investigate the fertilizing ability of human protamine-damaged sperm in a heterologous system using hamster oocytes. METHODS: The protamine of the sperm were damaged by exposure to dithiothreitol, a disulfide-reducing agent. Their ability to penetrate and form male pronuclei were investigated using the zona-free hamster penetration test and the hamster-intracytoplasmic sperm injection assay, respectively. RESULTS: The zona-free hamster penetration test revealed that protamine-damaged sperm are unable to bind and penetrate the hamster oocyte. On the other hand, hamster-intracytoplasmic sperm injection assay results showed that 56.9% and 39.2% of the injected oocytes developed male pronuclei in protamine-damaged and live-intact sperm groups, respectively, with a significant difference in these rates (P < 0.01). CONCLUSIONS: This study shows that protamine-damaged sperm are able to undergo sperm head decondensation and male pronuclear formation only when injected into the ooplasm, although they cannot bind and penetrate through the zona and enter the ooplasm.  (+info)

Reactive oxygen species-induced apoptosis and necrosis in bovine corneal endothelial cells. (3/413)

PURPOSE: The loss of corneal endothelial cells associated with aging and possibly other causes has been speculated to be related to exposure to reactive oxygen species (ROS). The current study was conducted to investigate, by use of photosensitizers, the underlying mechanisms involved in the death of bovine corneal endothelial cells (BCENs) caused by ROS. METHODS: BCEN cells in primary culture were treated with a photosensitizer (riboflavin or rose bengal) with light exposure. The patterns of cell damage and death were assessed using an acridine orange-ethidium bromide differential staining method, TdT-mediated dUTP nick-end labeling (TUNEL) assay, and transmission electron microscopy. The cytotoxicity was assayed by mitochondrial function using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) testing. Antioxidants, including catalase, L-histidine, salicylic acid, and superoxide dismutase, were used to determine the types of ROS involved. Activation of nuclear factor (NF)-kappaB was examined by fluorescent immunocytochemistry with anti-p65 antibody. RESULTS: Light-irradiated riboflavin or rose bengal resulted in a significant decrease in viability of BCEN cells. Chromosomal condensation and fragmentation were observed in apoptotic cells, and membrane lysis and damage of cell ultrastructures were observed in necrotic cells. Riboflavin induced apoptosis at 30 minutes and thereafter and induced necrosis after 2 hours. Rose bengal was shown to cause similar effects within half the time required for the effects of riboflavin. Catalase and salicylic acid were found to provide protection for BCENs from cytotoxic effects of riboflavin, and L-histidine was found to protect BCENs from cytotoxicity induced by rose bengal. Kinetic studies using immunocytochemistry showed that NF-kappaB was translocated into the nucleus within 15 minutes and 30 minutes after treatment with rose bengal and riboflavin, respectively. CONCLUSIONS: The cytotoxic effects of photo-irradiated riboflavin and rose bengal are shown to be mediated by two distinct but parallel pathways, one leading to apoptosis and the other to necrosis. Possible involvement of NF-kappaB in cell death is suggested. These findings provide potential leads for future investigation into the molecular mechanisms of loss of corneal endothelial cells related to aging, oxidative stress, and possibly other similar causes.  (+info)

Differential chemosensitivity of breast cancer cells to ganciclovir treatment following adenovirus-mediated herpes simplex virus thymidine kinase gene transfer. (4/413)

The development of resistance to radiation and chemotherapeutic agents that cause DNA damage is a major problem for the treatment of breast and other cancers. The p53 tumor suppressor gene plays a direct role in the signaling of cell cycle arrest and apoptosis in response to DNA damage, and p53 gene mutations have been correlated with increased resistance to DNA-damaging agents. Herpes simplex virus thymidine kinase (HSV-tk) gene transfer followed by ganciclovir (GCV) treatment is a novel tumor ablation strategy that has shown good success in a variety of experimental tumor models. However, GCV cytotoxicity is believed to be mediated by DNA damage-induced apoptosis, and the relationship between p53 gene status, p53-mediated apoptosis, and the sensitivity of human tumors to HSV-tk/GCV treatment has not been firmly established. To address this issue, we compared the therapeutic efficacy of adenovirus-mediated HSV-tk gene transfer and GCV treatment in two human breast cancer cell lines: MCF-7 cells, which express wild-type p53, and MDA-MB-468 cells, which express high levels of a mutant p53 (273 Arg-His). Treating MCF-7 cells with AdHSV-tk/GCV led to the predicted increase in endogenous p53 and p21WAF1/CIP1 protein levels, and apoptosis was observed in a significant proportion of the target cell population. However, treating MDA-MB-468 cells under the same conditions resulted in a much stronger apoptotic response in the absence of induction in p21WAF1/CIP1 protein levels. This latter result suggested that HSV-tk/GCV treatment can activate a strong p53-independent apoptotic response in tumor cells that lack functional p53. To confirm this observation, four additional human breast cancer cell lines expressing mutant p53 were examined. Although a significant degree of variability in GCV chemosensitivity was observed in these cell lines, all displayed a greater reduction in cell viability than MCF-7 or normal mammary cells treated under the same conditions. These results suggest that endogenous p53 status does not correlate with chemosensitivity to HSV-tk/GCV treatment. Furthermore, evidence for a p53-independent apoptotic response serves to extend the potential of this therapeutic strategy to tumors that express mutant p53 and that may have developed resistance to conventional genotoxic agents.  (+info)

Evanescent-wave microscopy: a new tool to gain insight into the control of transmitter release. (5/413)

Evanescent-wave excitation was used to visualize individual fluorescently labelled vesicles in an optical slice near the plasma membrane of bovine adrenal chromaffin cells. A standard upright microscope was modified to accommodate the optics used for directing a laser beam under a supracritical angle on to the glass-water interface on top of which the cells are grown. Whereas epi-illumination images appeared blurred and structureless, evanescent-wave excitation highlighted acridine orange-labelled vesicles as individual pinpoints. Three-dimensional (3D) trajectories of individual vesicles were obtained from time-resolved image stacks and used to characterize vesicles in terms of their average fluorescence F and mobility, expressed here as the 3D diffusion coefficient D(3). Based on the single-vesicle analysis, two groups of vesicles were identified. Transitions between these states were studied before and after stimulation of exocytosis by repetitive or maintained membrane depolarizations by elevated extracellular [K+]. Findings were interpreted as sequential transitions between the previously characterized pools of vesicles preceding the fusion step. The observed approach of vesicles to their docking sites was not explained in terms of free diffusion: most vesicles moved unidirectionally as if directed to their binding sites at the plasma membrane. Vesicle mobility at the membrane was low, such that the sites of docking and fusion were in close vicinity. Both the rim region and confined areas in the centre of the footprint region were the site of intense vesicle trafficking.  (+info)

Arrest of endosome acidification by bafilomycin A1 mimics insulin action on GLUT4 translocation in 3T3-L1 adipocytes. (6/413)

In insulin-sensitive fat and muscle cells, the major glucose transporter GLUT4 is constitutively sequestered in endosomal tubulovesicular membranes, and moves to the cell surface in response to insulin. While sequence information within GLUT4 appears to be responsible for its constitutive intracellular sequestration, the regulatory elements and mechanisms that enable this protein to achieve its unique sorting pattern under basal and insulin-stimulated conditions are poorly understood. We show here that arrest of endosome acidification in insulin-sensitive 3T3-L1 adipocytes by bafilomycin A1, a specific inhibitor of the vacuolar proton pump, results in the rapid and dose-dependent translocation of GLUT4 from the cell interior to the membrane surface; the effects of maximally stimulatory concentrations of bafilomycin A1 (400-800 nM) were equivalent to 50-65% of the effects of acute insulin treatment. Like insulin, bafilomycin A1 induced the redistribution of GLUT1 and Rab4, but not that of other proteins whose membrane localization has been shown to be insulin-insensitive. Studies to address the mechanism of this effect demonstrated that neither autophosphorylation nor internalization of the insulin receptor was altered by bafilomycin A1 treatment. Bafilomycin-induced GLUT4 translocation was not blocked by cell pretreatment with wortmannin. Taken together, these data indicate that arrest of endosome acidification mimics insulin action on GLUT4 and GLUT1 translocation by a mechanism distal to insulin receptor and phosphatidylinositol 3-kinase activation, and suggest an important role for endosomal pH in the membrane dynamics of the glucose transporters.  (+info)

Radiation-induced leukocyte entrapment in the rat retinal microcirculation. (7/413)

PURPOSE: To evaluate the effects of irradiation on leukocyte dynamics in the rat retinal microcirculation. METHODS: Thirty-five Brown-Norway rats received a dose of 10 Gy irradiation in one fraction. Leukocyte dynamics were studied with acridine orange digital fluorography, in which a nuclear fluorescent dye of acridine orange is used and examined by scanning laser ophthalmoscope. This technique allowed visualization of fluorescent leukocytes in vivo. The leukocyte dynamics were evaluated at 0, 4, 7, 14, 30, and 60 days after the irradiation. RESULTS: Mean leukocyte velocity in the retinal capillaries decreased immediately. It partially recovered on day 4 but then gradually decreased up to the 2-month mark. Low-dose irradiation induced entrapment of leukocytes in the retinal microcirculation. The number of entrapped leukocytes gradually increased with time. The major retinal vessels significantly constricted immediately after irradiation. The diameter was reduced by 76% in arteries and 75% in veins, 2 months after irradiation. CONCLUSIONS: Entrapped leukocytes may be activated and exacerbate vascular injury and microinfarction and thus may participate in the pathogenesis of radiation retinopathy.  (+info)

Tick-borne relapsing fever imported from West Africa: diagnosis by quantitative buffy coat analysis and in vitro culture of Borrelia crocidurae. (8/413)

West African tick-borne relapsing fever (TBRF) is difficult to diagnose due to the low number of spirochetes in the bloodstream of patients. Previously, the causative microorganism, Borrelia crocidurae, had never been cultured in vitro. TBRF was rapidly diagnosed for two patients returning from western Africa with fever of unknown origin by quantitative buffy coat (QBC) analysis. Diagnosis was confirmed by intraperitoneal inoculation of blood specimens from patients into laboratory mice. In vitro experiments showed that QBC analysis may be as much as 100-fold more sensitive than thick smear. Spirochetes were also cultured from blood samples from both patients in modified Kelly's medium and were identified as B. crocidurae by partial sequencing of the PCR-amplified rrs gene.  (+info)

Purified Mitochondrial Membrane Potential Assay Kit (Orange Fluorescence) from Creative Biomart. Mitochondrial Membrane Potential Assay Kit (Orange Fluorescence) can be used for research.
Purified Fixable Dead Cell Staining Kit (Orange Fluorescence 405) from Creative Biomart. Fixable Dead Cell Staining Kit (Orange Fluorescence 405) can be used for research.
Components: s2146 Acridine Orange Stock Solution s1656 Sorensens Phosphate Buffer pH 6.0 s2147 Calcium Chloride 0.1M Solution s100 Acetic Acid 1% Aqueous
For speedy, intra-operative pathological margin assessment to steer staged cancers excisions, multimodal confocal mosaic check picture wide surgical margins (approximately 1?cm) with sub-cellular resolution and mimic the appearance of conventional hematoxylin and eosin histopathology (H&E). The goal of this work is definitely to combine three confocal imaging modes: acridine orange fluorescence (AO) for labeling nuclei, […]. ...
We do routinely use acridine orange using FACScan. A 20 min rinse with 10 times water-diluted Chlorox bleach is adequate to clean the instrument. Zbigniew Darzynkiewicz -----Original Message----- From: Karen Creswell [mailto:creswelk at georgetown.edu] Sent: Friday, June 04, 2004 1:17 PM To: cyto-inbox Subject: Acridine Orange Does anyone routinely run acridine orange through their FACScan/FACSort? I would like suggestions for cleaning the instrument afterwards. Thanks for your help, Karen -- Karen Creswell, Ph.D. Director, Flow Cytometry/Cell Sorting Shared Resource Lombardi Cancer Center Georgetown University Medical Center Research Bldg W304 Box 571469 Washington, DC 20057-1469 202-687-2233 creswelk at georgetown.edu ...
Acridine Orange hemi(zinc chloride) salt from Sigma-Aldrich,Application: Acridine orange has been used as a fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Acridine orange at a concentration of 120 μM will detect purified DNA in gels with a sensitivity of 25-50 ng per band. It has also been used extensively for cell staining of,biological,biology supply,biology supplies,biology product
The adsorption of the metachromic dye acridine orange (AO) by Na-beidellite was investigated by visible spectroscopy. Different types of clay-AO association were identified from the appearance and location of absorption bands alpha or beta . The colloidal properties were determined from curves of the absorbance vs. the degree of saturation. Three regions were identified in the absorbance curve. In the first region beidellite is peptized with small amounts of AO and the dye penetrates into the interlayer space where it undergoes metachromasy due to pi interactions between the aromatic entity and the oxygen plane of the clay. With larger amounts of AO (second region), the clay flocculates due to the aggregation of the dye cations in the interparticle space of the flocs. In excess AO (third region), beidellite is gradually peptized, forming small tactoids with monomeric AO in the interlayer space and at the same time adsorbing dimeric and polymeric AO cationic species at the solid-liquid interface. ...
Sixteen marine isolates from a NORPAX cruise, which were transferred once on medium after initial isolation, survived nutrient deprivation for at least 8 months (longest period test). All but one isolate remained cellularly intact, although their sizes and shapes changed greatly, and all became smaller, decreasing in size from 40 to 79%. Three starvation-survival patterns were demonstrated, namely (i) an initial increase in viable cells followed by a decrease until a constant number was reached, (ii) an increase in viable cells until a constant number was reached, and (iii) a decrease in viable cells until a constant number was reached. One isolate from each starvation-survival pattern was starved for 8 months and then was tested in comparison with 4-month-starved Ant-300 for [14C]glutamic acid uptake, respiration, and incorporation. The response to glutamic acid was rapid and linear in each case. The data indicate that the starvation-survival of Ant-300 is not an anomalous situation and that ...
BioAssay record AID 693243 submitted by ChEMBL: Induction of apoptosis in human MGC803 cells assessed as pyknosis at 5 uM after 12 to 48 hrs by acridine orange/ethidium bromide staining.
Acridine Orange: A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.
DNA intercalating dye. A grade of acridine orange of exceptionally high purity, suitable for quantitative work. Free of inorganic salts.
HeLa cells. Light micrograph (acridine orange stain) of a culture of HeLa cancer cells. The nuclei of the cells are stained yellow while the cytoplasm is orange. HeLa cells are the first human cell line established in 1952 to research cancer. They were obtained from the cervix of Henrietta Lacks (after whom the cells are named) from Baltimore, USA, in 1951, who died of cervical cancer eight months later. HeLa cells thrive unusually well in the laboratory and are used in research worldwide. Professor Anderson (1911-2006) was the first to use acridine orange staining to study cells, bacteria and viruses. - Stock Image G442/0059
Background: Mitochondrial permeabilization provokes cardiomyocyte death, and causes adverse left ventricular remodeling in cardiac-restricted TNF transgenic mice (MHCsTNF). Autophagy, a lysosomal degradative pathway, is essential for removal of ubiquitin-tagged damaged mitochondria. Our prior work suggests that TNF receptor associated factor-2 (TRAF2), a multifunctional protein with E3 ubiquitin ligase activity, facilitates removal of damaged mitochondria. The role of TRAF2 vis-à-vis PARKIN, another E3 ubiquitin ligase essential for mitophagy, is unknown.. Methods and Results: MHCsTNF hearts and TNF-treated neonatal rat cardiac myocytes (NRCMs) demonstrate upregulation of both TRAF2 and PARKIN, with increased evidence for mitochondrial autophagy and reduced mitochondrial mass. To evaluate the role of TRAF2 and PARKIN in autophagic removal of damaged mitochondria, we employed gain-of-function and loss-of-function approaches; and assessed mitochondrial mass with nonyl-acridine orange fluorescence ...
Stain F-actin in cell samples with F-actin Staining Kit - Orange Fluorescence - Cytopainter ab112126. For fluorescence microscopes.
A while ago, scientists at the National University of Singapore created transgenic, fluorescent zebra fish by injecting red, green, and orange fluorescence genes from a jellyfish into the fish eggs. They did this so the fish could help detect environmental pollutants.Now, you can actually have them for pets! They are marketed under the name GloFish, and look very cool under a black light. Link - Thanks moniA!Previously on Neatorama: Fluorescent pig, rabbit, butterfly, and frog...
The metal chelating compound Dp44mT is a di-2-pyridylketone thiosemicarbazone (DpT) which displays potent and selective anti-tumor activity. This compound is receiving translational attention but its mechanism is poorly understood. Here we report that Dp44mT targets lysosome integrity through copper binding. Studies using the lysosomotropic fluorochrome acridine orange established that the copper-Dp44mT complex (Cu[Dp44mT]) disrupted lysosomes. This targeting was confirmed with pepstatin A-BODIPY FL, which showed re-distribution of cathepsin D to the cytosol with ensuing cleavage of the pro-apoptotic BH3 protein Bid. Redox activity of Cu[Dp44mT] caused cellular depletion of glutathione and lysosomal damage was prevented by co-treatment with the glutathione precursor N-acetylcysteine. Copper binding was essential for the potent anti-tumor activity of Dp44mT, since co-incubation with non-toxic copper chelators markedly attenuated its cytotoxicity. Taken together, our studies show how the lysosomal ...
The majority of phosphatase (PO4ase) activity detected in fresh aerobic activated sludge from a municipal wastewater treatment plant was associated with suspended floc material. PO4ase activity appeared to be localized in discrete bacteria-containing areas of the floc matrix based on the distribution of nucleic acid-stained cells and precipitated fluorescent crystals produced as a result of reaction of the enzyme(s) with the artificial substrate ELF™-PO4. Of the total floc-associated bacterial cells that stained positive with the nucleic acid-binding fluorochrome acridine orange (AO), 8.8 ± 1.2% displayed PO4ase activity based on the proximity of AO-stained cells to precipitated ELF crystals. Using a 16S rRNA oligonucleotide probe specific for the cytophaga-flavobacteria group, it was determined that 17-20% of the floc-associated bacteria that probed positive also displayed PO4ase activity. Furthermore, 35-45% of the ELF fluorescence was associated with bacterial cells that probed positive ...
Name:C.I.Basic Orange 14,C.I.46005 Molecular Structure: Acridines class C.I.Basic Orange 14,C.I.46005,CAS 65-61-2,301.81,C17H20ClN3,Acridine Orange C.I.Basic Orange 14,C.I.46005,CAS 65-61-2,301.81,C17H20ClN3,Acridine Orange Molecular Formula:C17H20ClN3 Molecular Weight: 301.81 CAS Registry Number:65-61-2
Aims: In view of recent findings that a multidrug efflux pump CmeABC exists in Campylobacter jejuni, 391 C. jejuni and 52 Campylobacter coli of human and animal origin were examined for a multidrug resistance phenotype.. Materials and methods: The MICs of ampicillin, chloramphenicol, ciprofloxacin, erythromycin, kanamycin, tetracycline, cetrimide, triclosan, acridine orange, paraquat and ethidium bromide were determined. Resistance to organic solvents and the effect of salicylate (known inducer of the marRAB operon in Escherichia coli and Salmonella) were also examined.. Results: Two C. coli and 13 C. jejuni isolates, mainly from pigs or poultry, were resistant to three or more antibiotics and 12 of these strains had reduced susceptibility to acridine orange and/or ethidium bromide. Strains (n = 20) that were less susceptible to acridine orange, ethidium bromide and triclosan were significantly more resistant (P , 0.05) to ampicillin, chloramphenicol, ciprofloxacin, erythromycin, nalidixic acid ...
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Acridine orange is a nucleic acid staining dye that is used to identify dead cells. It intercalates with the double-stranded DNA base pairs at a ratio of 1:3 and is capable of emitting green fluorescence (Ex: 500 nm, Em: 520 nm). It also emits red fluorescence (Ex: 460 nm, Em: 650 nm) when bound to RNA or single-stranded DNA. Since acridine orange is mutagenic, this product is supplied as a solution that prevents it from splashing during weighing.. ...
During human spermiogenesis, the elongated spermatids undergo a plasma membrane remodeling step that facilitates formation of the zona pellucida and hyaluronic acid (HA) binding sites. Various biochemical sperm markers indicated that human sperm bound to HA exhibit attributes similar to that of zona …
The accuracy of techniques for the diagnosis of malaria are usually compared with optical microscopy, which is considered to be a gold standard. However, microscopy is prone to error and therefore makes it difficult to assess the reliability of other diagnostic techniques. We did a systematic review to assess the specificity and sensitivity of diagnostic techniques in different settings, using a statistical method that avoided defining a gold standard. Performance varied depending on species of the malaria parasite, level of parasitaemia, and immunity. Overall, histidine-rich protein 2 (HRP2)-based dipsticks showed a high sensitivity (92.7%) and specificity (99.2%) for Plasmodium falciparum in endemic areas. The acridine orange test was more sensitive (97.1%) in detecting P falciparum in epidemiological studies, with a specificity of 97.9%. In the absence of a gold standard, HRP2 dipsticks and acridine orange could provide an alternative for detecting falciparum infections in endemic areas and
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Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
PromoKine offers a wide range of dyes for fluorescently staining dead cells. The membrane-impermeable nuclear stains can also be used for fixed cells. Commonly used dyes for staining dead cells include; orange-fluorescent ethidium bromide (EB), red-fluorescent propidium iodide (PI), green-fluorescent acridine orange (AO) and blue-fluorescent DAPI. The toluidine derivative Trypan Blue is a classical non-fluorescent dye used to selectively stain dead cells and shows a distinctive blue colour under the microscope. ...
A quiescent [denoted as Q(G0/G1)] subpopulation was isolated from EMT6/Ro-fed plateau monolayers by centrifugal elutriation. The median Coulter volume of these cells was significantly smaller than that of the original population from which they were elutriated. Using two-step acridine orange staining and dual parameter flow cytometric analysis, over 95% of quiescent cells were found to have G1 DNA content, and 80% of the cells had a decreased RNA content as compared to rapidly proliferating exponential G1 cells. After labeling for 24 hr (two doubling times) with [3H]thymidine, less than 2% of the quiescent cells incorporated [3H]thymidine as measured by autoradiography. The colony-forming efficiency of these cells was not significantly different from that of exponential cells. When such Q(G0/G1) cells were replated in fresh medium at a lower density, there was a lag time of 30 hr before any increase in cell number was detected, after which the cell-doubling rate matched that of exponential ...
contact Luis Benitez at Oncological Research Unit, kAP 107-014, UNAM, Hosp. 21 Sieglo, Mexico DF 06700, Mexico. He has some interesting results along this line. In article ,905kf544 at invrof.anmat.sld.ar, elisa at invrof.anmat.sld.ar (Dra. Elisa Bal de Kier-Joffe) writes: ,Dear netters: ,We are using Acridine Orange (AO) to stain apoptotic cells. , ,The nuclei staining of apoptotic cells is very easily recognized when it is ,in an advanced stage. ,However when the process is early I observe only a brighter staining of ,chromatin clumps, and a more marked limit in nuclei periphery. When this ,happens, the cytoplasm shows a diffuse red staining which is also evident in ,definite apoptotic cells. , ,In contrast normal alive cells only show a pale diffuse green staining of ,the nuclei and cytoplasm and only a kind of red granule (RNA) in the ,cytoplasm next to the nucleus. , ,I have not found any comments concerning this early diffuse intense red ,staining of the cytoplasm with AO in apoptotic ...
A novel method for the detection, screening, the quantitation of compounds that interact with nucleic acids (DNA or RNA) is provided. The basis for this method is the inhibition of acridine orange binding to the nucleic acid. Measurement of binding inhibition utilizes the rapid, repeatable technique of fluorescence polarization.
Fluorescence filter set EX480 (BP30) DM505(LP) EM535 (BP40) optimized for Acridine Orange + DNA, Alexa Fluor 488™, Atto 488, Azami Green, BODIPY FL/pH7.2, Calcein, Calcium, Green™-1, Cy2™, DiO, DyLight 488, EGFP, Emerald GFP, FAM, FITC, Fluo-4, GFP, LysoTracker Green/pH 5.2, MitoTracker Green, FM/MeOH, mWasabi, Oregon Green™ 488, SYBR® Green I, SYTO 9/DNA, ZsGreen fluorophore. Needs empty filter block (OX.9749 ...
Fluorescent micrographs and quantitative analysis of CAOV-3 cells double-stained with acridine orange and propidium iodide. Cells were treated with liriodenine
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To peel the oranges cut both ends and place the orange on one end on a cutting board. Using a sharp knife shave the peel off downwards all the way around, making sure to also remove the thin white skin around the orange. Chop the orange into small pieces. Weigh the chopped orange. ...
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Fruit can be divided into a good many varieties; especially oranges are popular fruit. Oranges are eaten by many ways; such as the orange drink. The process of manufacturing orange drink can be divided into four stages: picking and preparation of the f...
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Deep, Dark Salted Butter Caramel Sauce, for serving (after using Zoe Nathans, decided that I definitely prefer my own, reprinted below). 1. In a food processor, pulse the 1 cup of flour with 2 tablespoons of the sugar and the baking powder and salt. Add the stick of cold butter and pulse several times, just until it is the size of peas. Sprinkle the dough with the ice water and pulse just until moistened crumbs form. Turn the crumbs out onto a work surface, knead once or twice and pat the pastry into a disk. Wrap the pastry in plastic and chill for 30 minutes.. 2. On a floured work surface, roll out the pastry to an 11-inch round, about 1/4 inch thick. Transfer the pastry to a parchment paper-lined flat cookie sheet and refrigerate for 15 minutes, or until chilled.. 3. Meanwhile, peel the blood oranges, removing all of the bitter white pith. Thinly slice 2 of the oranges crosswise; remove the pits. Transfer the orange slices to a plate. Working over a sieve set over a bowl, cut in between the ...
Deep, Dark Salted Butter Caramel Sauce, for serving (after using Zoe Nathans, decided that I definitely prefer my own, reprinted below). 1. In a food processor, pulse the 1 cup of flour with 2 tablespoons of the sugar and the baking powder and salt. Add the stick of cold butter and pulse several times, just until it is the size of peas. Sprinkle the dough with the ice water and pulse just until moistened crumbs form. Turn the crumbs out onto a work surface, knead once or twice and pat the pastry into a disk. Wrap the pastry in plastic and chill for 30 minutes.. 2. On a floured work surface, roll out the pastry to an 11-inch round, about 1/4 inch thick. Transfer the pastry to a parchment paper-lined flat cookie sheet and refrigerate for 15 minutes, or until chilled.. 3. Meanwhile, peel the blood oranges, removing all of the bitter white pith. Thinly slice 2 of the oranges crosswise; remove the pits. Transfer the orange slices to a plate. Working over a sieve set over a bowl, cut in between the ...
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Youre in the right place if you want to avoid the orange, and who doesnt. Lets begin by explaining how skin can turn orange. Over-application (that can be too much in one application and/or applying too frequently), using budget brands that use lower-quality ingredients, and applying when your skin is noticeably dry. Follow these tips to lessen the dreaded orange:. ...
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What does God want for your life? What is Gods plan for you? Want some orange drink? These were the questions people asked me on Sundays when I was growing up as a teenager in church. We wondered about what God wanted for us, and wondered how we all could hold ourselves and our friends accountable for our behavior to keep us walking in the path of holiness. All, of course, discussed over the ubiquitous orange drink served at youth meetings across the country. Orange drink is the most righteous of bulk beverages.
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For rapid, intra-operative pathological margin assessment to guide staged cancer excisions, multimodal confocal mosaic scan image wide surgical margins (approximately 1 cm) with sub-cellular resolution and mimic the appearance of conventional hematoxylin and eosin histopathology (H&E). The goal of this work is to combine three confocal imaging modes: acridine orange fluorescence (AO) for labeling nuclei, eosin fluorescence (Eo) for labeling cytoplasm, and endogenous reflectance (R) for marking collagen and keratin. Absorption contrast is achieved by alternating the excitation wavelength: 488 nm (AO fluorescence) and 532 nm (Eo fluorescence). Superposition and false-coloring of these modes mimics H&E, enabling detection of cutaneous squamous cell carcinomas (SCC). The sum of mosaic Eo þ R is false-colored pink to mimic the appearance of eosin, while the AO mosaic is false-colored purple to mimic the appearance of hematoxylin in H&E. In this study, mosaics of 10 Mohs surgical excisions containing ...
For rapid, intra-operative pathological margin assessment to guide staged cancer excisions, multimodal confocal mosaic scan image wide surgical margins (approximately 1 cm) with sub-cellular resolution and mimic the appearance of conventional hematoxylin and eosin histopathology (H&E). The goal of this work is to combine three confocal imaging modes: acridine orange fluorescence (AO) for labeling nuclei, eosin fluorescence (Eo) for labeling cytoplasm, and endogenous reflectance (R) for marking collagen and keratin. Absorption contrast is achieved by alternating the excitation wavelength: 488 nm (AO fluorescence) and 532 nm (Eo fluorescence). Superposition and false-coloring of these modes mimics H&E, enabling detection of cutaneous squamous cell carcinomas (SCC). The sum of mosaic Eo þ R is false-colored pink to mimic the appearance of eosin, while the AO mosaic is false-colored purple to mimic the appearance of hematoxylin in H&E. In this study, mosaics of 10 Mohs surgical excisions containing ...
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Publications and Work Accepted for Publication:. A. Books. - Poli A., Fabbri E., Agnisola C., Calamita G., Santovito G., Verri T. Fisiologia Animale. Edises, Napoli, Italy, pp. 1-633, 2014. - Poli A., Fabbri E., Agnisola C., Calamita G., Santovito G., Verri T. Fisiologia Animale(II edizione).Edises, Napoli, Italy,pp. 1-720, 2018. B. Articles in Scholarly Journals. - Verri T.*, Maffia M., Storelli C. H+/glycyl-glycine cotransport in eel intestinal brush border membrane vesicles: studies with the pH-sensitive dye acridine orange. Biochim. Biophys. Acta 1110(1): 123-126, 1992. I.F.(1992)=2.610. - Marsigliante S., Verri T., Barker S., Jimenez E., Vinson G.P., Storelli C. Angiotensin II receptor subtypes in eel (Anguilla anguilla). J. Mol. Endocrinol. 12(1): 61-69, 1994. I.F.(1994)=2.232. - Perego C., Markovich D., Norbis F., Verri T., Sorribas V., Murer H. Expression of rat ileal Na+-sulphate cotransport in Xenopus laevis oocytes: functional characterization. Pflügers Arch. - Eur. J. Physiol. ...
Cara Cara oranges are a type of navel orange that has been steadily growing in popularity over the past few years. The oranges are a hybrid between the Washington navel orange and the Brazilian Bahia navel orange, and they were first discovered in 1976 in Venezuela at a place called Hacienda Cara Cara, which is where their name comes from. The Cara Caras look similar to regular navel oranges from the outside, but they have a distinctive pink color to their flesh that is immediately noticeable when you cut into one. The color is similar to that of a ruby red grapefruit, but ranges from a pale pink to a much darker shade from orange to orange. They are sweet, juicy and have a very low acidity. They are also noted for having a more complex flavor than your average orange, with notes of cranberry, grapefruit or even tart cherries in with the orange flavor.. Cara Caras can be used in any recipe that calls for oranges, whether it is in the form of orange juice or orange zest. They are very popular ...
氯乙烯類化合物廣泛應用於乾洗及金屬除油,但因不當處置或意外滲漏,使氯乙烯類化合物污染土壤與地下水體,又因氯乙烯類污染物具高毒性,對環境與民眾健康構成嚴重威脅。基於環境友善與經濟效益,生物復育是目前推崇的整治方法之一,但採用生物復育策略之前,須對污染場址微生物菌相,甚至目標污染物之降解功能性基因與菌株有一定的掌握,以瞭解場址是否具有生物整治潛勢。高氯數氯乙烯類化合物的降解主要以厭氧還原脫氯為主,著名菌屬Dehalococcoides不同菌種具有不同還原脫氯功能性基因(vcrA、bvcA與tceA),本研究針對污染場址的菌屬Dehalococcoides暨其還原脫氯功能性基因進行分析與定量;另使用次世代定序技術分析場址枯、豐水期菌相是否產生差異,特別是還原脫氯及產氫菌群之變化;並使用螢光染劑Acridine orange (AO
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Ive been on an orange kick lately (I dont normally eat oranges), and I randomly googled oranges. I found this article in the Washington Post a few days ago about a bacterial infection plaguing oranges in Florida, huanglongbing. Im guessing the price of oranges will go up. I was reminiscing recently about the best oranges…
The How to make your very own Orange Man video is below). As I said in my last post….this is the shit that my mind comes up with in the wee hours of the night. Orange Man appeared in my life one day when Shawn sent me a picture of Orange Man hanging out in a coffee shop. Then later sitting on a shelf, and lastly face down on a keyboard tired from a hard day at work (pic up above.). Last week I shared a few on social media. AND then…..I woke up in middle of the night thinking…..hey, ORANGE is the color used for MS - Sooooo Orange Man should SUPPORT MS!!!. And…Orange Mans presence in the MS community began to take root.. FIRST there was his Twitter handle: @OrangeMan4MS. THEN his very own Instagram account: orangeman4ms. ...
NFC, which dominates juice sales and is most associated with all thats pure and simple, is indeed a processed product. Its story involves chemistry, globalization, and questions of land use. Oranges undergo what the industry calls hard extraction (not a gentle squeeze). Extracted juice is vacuum-stripped of oxygen, de-oiled, and held in massive tanks (holding as much as 1.5 million gallons), sometimes for more than a year, before being made palatable via a flavor pack, a proprietary blend of orange essence and orange oil, themselves composed of hundreds of chemicals. (Ethyl butyrate is the one that most pleases North American palates.) The flavor pack is water-soluble, which means that some water goes in with it. But its still Florida orange juice, right? Not really: as Florida growers lose land to real-estate developers, more and more oranges and essence and oil come from Brazil, where land and labor are cheaper and environmental regulation minimal ...
New for HPI Formula Ten cars is this aluminum motor mount. It replaces the stock plastic motor mount with a precision machined aluminum mount for extra rear pod stiffness, extra motor cooling and more durability. The mount is anodized bright orange for a custom look that matches other Formula Ten option parts.. Mounting hardware is included. Check out the full range of performance option parts for the Formula Ten! #61644 SERVO SAVER SET (Cyclone 12) #102877 BALL BEARING SET (FORMULA TEN) #102878 BALL DIFFERENTIAL SET (95 TOOTH/64 PITCH) #102879 THREADED ALUMINUM SHOCK SET #102908 ALUMINUM HEX WHEEL HUB (ORANGE/2pcs) #102909 ALUMINUM FLUORINE COATED FRONT WHEEL SHAFT (2pcs) #102910 ALUMINUM SERVO MOUNT SET (ORANGE) #102911 ALUMINUM MOTOR MOUNT (ORANGE) #102912 ALUMINUM LEFT BULKHEAD (ORANGE) #103018 ALUMINUM REAR AXLE SHAFT 6.3x130mm (ORANGE) #103016 BRIDGESTONE HIGH GRIP FT01 SLICK TIRE M (FRONT) #103017 BRIDGESTONE HIGH GRIP FT01 SLICK TIRE M (REAR) #102906 BRIDGESTONE HIGH GRIP FT01 SLICK TIRE ...
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Current chemotherapies for solid tumors remain severely limited due to toxicity and the development of drug resistance. Hence, new agents are vital to explore in order to improve patient outcomes.. We have identified the novel chelator Dp44mT as a potent and selective anti‐tumor agent that overcomes resistance to standard chemotherapeutics (Whitnall, M., Howard, J., Ponka P. and Richardson, D.R. PNAS USA 2006; 103:14901-6).. To understand its mechanism of action and potential anti‐angiogenic activity, we have investigated its ability to interact with cellular copper (Cu). In contrast to its marked ability to potently mobilize cellular 59Fe, Dp44mT caused marked intracellular 64Cu accumulation. Cellular fractionation demonstrated that 64Cu was concentrated in a fraction containing lysosomes and mitochondria. Moreover, the lysosomotropic character of Dp44mT suggested that it may become concentrated in lysosomes. To test this, studies with the lysosomotropic agent, acridine orange, demonstrated ...
Bullacta exarata was hydrolyzed with trypsin to prepare peptides; Hydrolysates were isolated by ultrafiltration and purified using G-25 gel filtration. The purity of the Bullacta exarata was demonstrated by HPLC and its peptide sequence analysis was detected. The effects of BEPT II and BEPT II-1 on the proliferation of PC-3 cells were examined using a MTT assay. BEPT II and BEPT II-1 significantly inhibited the proliferation of PC-3 cells in a time- and dose-dependent manner. Annexin V/PI double staining studies showed exposing PC-3 cells to 5, or 15 mg/mL BEPT II-1 for 24 h increased the percentage of the early stage of apoptotic cells from 11.22% to 22.09%. In addition, typical morphologic changes were observed in the cells with acridine orange/ethidium bromide staining. These data support that BEPT II-1 has anticancer properties and merits further investigation to understand the mechanisms of BEPT II-1-induced apoptosis in PC-3 cells.
Diabetes was induced in rats by administration of a single i.p. injection of streptozotocin (50 mg/kg body wt). After 7 d, diabetic rats were further treated with insulin or 1,25-dihydroxycholecalciferol [1,25(OH)2D3] for an additional 5-7 d. Control, diabetic, diabetic + insulin, and diabetic + 1,25(OH)2D3 rats were then killed, their proximal small intestines were removed, and villus-tip epithelial cells were isolated and used to prepare brush-border membrane vesicles. Preparations from each of these groups were then analyzed and compared with respect to their amiloride-sensitive, electroneutral Na(+)-H+ exchange activity, using 22Na uptake as well as acridine orange techniques. The results of these experiments demonstrated that (a) H+ gradient-dependent 22Na uptake as well as Na+ gradient-dependent transmembrane H+ fluxes were significantly increased in diabetic vesicles compared to their control counterparts, (b) kinetic studies demonstrated that this enhanced 22Na uptake in diabetes was a ...
Orange discoloration anywhere in the body is not common. For example, there may be a yellowish discoloration of the skin as in jaundice but most of the time we would not expect an orange hue to the skin. It can however occur like with tanning sprays and colorants (fake tans). In fact some of the causes of orange stool may also contribute to a yellow to orange hue of the skin.. Normally stool is a brown color and varies from a tan hue to dark brown in color. This color is due to an accumulation of undigested food, wastes and other substances that are retained in the gut to be expelled with a bowel movement. One of the major contributors of the characteristic brown color of stool is a substance known as stercobilin which is derived from bilirubin (a strong yellow colored pigment produced by the liver).. Bile is stored in the gallbladder and released when food is being digested, especially with the presence of fatty foods, as bile emulsifies fats to help with digestion. If there is any problem ...
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In the same saucepan, mix the water, 1 cup of the sugar and orange juice and cook over medium heat for about a minute, stirring occasionally, until the sugar melts. Add the orange zest and bring to a boil over medium-high heat. Reduce the heat to medium-low and simmer for about 10 minutes. Remove from the heat and let the zest cool in the syrup to room temperature.. Drain the zest and reserve the syrup for another use. Transfer the zest to a small bowl and sprinkle with the remaining tablespoon of sugar. Toss to coat and then let the zest stand at room temperature for about 2 hours to give it time to cool and absorb the sugar. Use right away or store in an airtight container for up to 24 hours.. To make the mousse: Fill a large bowl with ice and water and keep it close at hand.. In a saucepan, sprinkle the gelatin over the cold water and let it sit for about 4 minutes to soften. Add the boiling water and stir until the gelatin dissolves.. Stir in the orange juice, sugar and salt. If the mixture ...
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The newfound source of income is fast replacing orange as the main cash crop. Agriculture: The festive mood is still on after Dasain, but farmer Kiran Rai from Dunglagang, Tsirang is already out in the field tending to his chili seedlings. There is no time to waste as he is trying to beat others to produce the first local chilies.. Not long ago, farmers like Kiran Rai would sit back waiting for middlemen to roam their orange orchards. Orange or citrus mandarin was the main cash crop and farmers turned whatever land they owned into orange orchards.. This is changing.. With most of the trees in warmer places dying or dead, Tsirang farmers are slowly but increasingly turning to vegetables. It is a good and reliable source of income.. Tsirang farmers are as busy as bees.. Everyday is important for Kiran. The first produce fetches the best price, says Kiran. Last year, he produced about 10 quintals of chilli, which fetched him about Nu 70,000. Some farmers earn between Nu 100,000 and 150,000 ...
Hi Jess...again, not a holiday, but we just wrapped up the 2008 Orange Conference in Atlanta on April 30. I have been following your blog for a while just getting ideas because Im the conference director for the Orange Conference. Its hosted by the reThink Group, and were an organization that equips and trains churches about family ministry. Its the whole idea that if red represents the family (the color of blood and warmth) and yellow represents the church (like light), then when these two work together to reach the family, you get a brilliant, beautiful orange. Fun stuff...www.theorangeconference.com. Thanks for a great blog ...
The Stent Placement Kit Orange is stainless steel and contains three parts: Stent Introducer Tube, Stent Plunger and Stent Loader all of which possess the orange identification ring. The orange kit should be used in conjunction with the orange bronchial tube when placing stents 16 -18 mm and all Y-Stents.. Product URL : http://www.bryancorp.com/stent-placement-kit.cfm. Last Updated : 11/09/2012. Categories : Stents, Tracheal ...
I made the smaller of the two wedding cakes in The Book for my graduation party, and this was the frosting that went with the recipe. I love frosting. I particularly love mousseline buttercreams, so I was quite optimistic about this recipe. Much to my dismay though, this frosting was bad. The texture was lovely, as it always is in mousseline buttercream, so it was really the flavor that was the problem. Instead of tasting like orange (as you might have guessed from the name) this frosting tasted only of one thing: butter! Now, I like butter, but this frosting tasted just like whipped butter, which isnt particularly delicious on cake. It was especially frustrating because this recipe was seriously time-consuming. Mousseline buttercream takes a long time, and this one also had orange curd folded in. The orange curd tasted good before it was added to the frosting, but the orange flavor of it got lost behind all the butter in the final product. The cake that this frosting went with was excellent, ...
Can PKD patients eat orange and lemon? Kidney patients are very worried about the food they eat. Eating improperly will worsen their condition. So is orange and lemon good for PKD patients? First, lets see the benefits of orange. Orange can prevent k
The freeMD virtual doctor has found 18 conditions that can cause Coughing Mucus and Pee is Too Orange. There is 1 somewhat common condition that can cause Coughing Mucus and Pee is Too Orange. There are 4 uncommon conditions that can cause Coughing Mucus and Pee is Too Orange. There are 13 rare conditions that can cause Coughing Mucus and Pee is Too Orange.
Detect 4 Drugs (D4D) is an aerosol reagent packaged as a single spray canister, yet enables the user to detect FOUR (4) types of drugs in a single test session! The product is a 100-test size canister that is able to make a preliminary detection and identification of 4 drugs from a single trace collection. Wiping any surface once with the Special Test paper #630, and spraying with D4D will reveal traces of: Hashish or Marijuana, as reddish brown stains; Heroin, as purple gray stains; Amphetamine, as canary-yellow stains; and Meth-amphetamines, as orange stains. D4D is available in kit #1002, consisting of one aerosol canister of DETECT 4 DRUGS, two dispensers of #630 test papers, one aerosol canister of COCA-TEST (for detecting and identifying Cocaine/Crack) and two dispensers of #530 collection papers. This configuration covers most of the common illicit drugs used ...
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As you take a sip of White Claw Hard Seltzer Surge Blood Orange, a potent, unmistakable blood orange taste bursts through, perfectly balanced with the right acidity and a hint of sweetness. Not overpowering, the new flavor is mouth-watering, like a mixed drink muddled with fresh blood orange.
Healthcare trade publication Beckers Infection Control & Clinical Quality has listed Orange Regional Medical Center in Middletown among the top 49 hospitals in the nation with the lowest death rates in the 30-days after hospital care for heart attack. According to data from The Centers for Medicare and Medicaid Services (CMS), Orange Regionals rate of 11.4 percent puts the hospital better than the national average of 14.1 percent. All of the hospitals on the list have a 30-day mortality rate from heart attack of 11.5 percent or less. The figures are from CMS Hospital Compare website and represent data from July 2012 through June 2015, the most recent information available. CMS consider 30-day mortality rates to be an outcome of care measure. The evaluation of an outcome using a standardized period of assessment complies with the standards for such measures as articulated in a scientific statement by the American College of Cardiology and the American Heart Association. Rose Baczewski, Chief Quality
This orange ribbon tac pin has an orange ribbon and represents leukemia. The orange cancer pin makes a wonderful gift for someone going through treatment.
Orange Coast, the magazine of Orange County, showcases monthly coverage of the people, places and events throughout the community. As well, we offer a variety of print and digital products and custom content. Orange Coast - Premiere OC - Design OC - OC Eats - Pasadena ...
With the arrival of winter we begin to put on the table the oranges. Delicious, juicy, oranges enjoy many properties, in particular red oranges than other
Palestine Remembered (فلسطين في الذاكرة)/14193: Jaffa-Jaffa: Before nailing the orange boxes, and after quality control, each orange is carefully wrapped. Matson Collection /14193-Pictures, Stories, Nakba Oral hsitory, Palestine Satellite & Street View, and maps
[vc_row][vc_column][vc_column_text]Critical Orange Punch is a feminized photoperiod seed variety, the first Dutch Passion strain based on outstanding Kritikal Bilbo genetics from Spain. The breeding began by crossing elite cuttings of Grandaddy Purps and Orange Bud (Orange Punch). The result was a supreme smoke with great taste but below-average yields. When this was further crossed with... Read More
Illumalite Designs Orange Crackle Accent Lamp. Striking shades of orange and rust create a beautiful look on this lamp. Sure to be a focal point of any room. The lamps use maximum 60 watt bulb, not included. Easy on/off switch located on the cord. Shades of orange and rust wrap around entire lamp. Shade sits atop solid wooden base. Made in the USA.
Orange is a city located in Orange County, California. It is approximately 3 miles (4.8 kilometers) north of the county seat, Santa Ana. Orange is unusual
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by admin , Jan 29, 2016 , Articles. Ever have a craving for an orange? or a clementine? or any citrus fruit for that matter? Theres a reason for it. Not only because that orange tastes delicious but because of the rich profile of nutrients it contains that feed your cells. Oranges are beneficial ...
Orange Gravity 2: Level Pack: The orange is back and trickier than ever in this pack of all new levels for Tommycreds physics puzzler. Using ropes, gravity, and a variety of mouse-driven pulleys, switches, cranks and more, get your orange to nab each lemon on the stage and then safely to the exit. Its harder than you might think.
Oranges are my fruit of the season. What better way to start your day than a ripe, sweet orange? I just love the taste of an orange - slightly sour,...
Acridine orange[edit]. Acridine orange (AO) is a nucleic acid selective fluorescent cationic dye useful for cell cycle ... The stain may also be used in conjunction with acridine orange (AO) in viable cell counting. This EB/AO combined stain causes ... It uses a combination of haematoxylin, Orange G, eosin Y, Light Green SF yellowish, and sometimes Bismarck Brown Y. ... In a skilfully made H & E preparation the red blood cells are almost orange, and collagen and cytoplasm (especially muscle) ...
... also binds to acridine orange. "Duranat Cu Chlorophyllin WSP (Natural Green 3-1 CI 75810-1)". Retrieved 15 ...
Acridine derivatives: proflavin, acridine orange, acridine yellow, etc. Arylmethine derivatives: auramine, crystal violet, ... DRAQ7 and CyTRAK Orange Pyrene derivatives: cascade blue, etc. Oxazine derivatives: Nile red, Nile blue, cresyl violet, oxazine ...
Acta 883: 173-177 Notes Darzynkiewicz Z, Kapuscinski J. (1990)"Acridine Orange, a Versatile Probe of Nucleic Acids and Other ... Another example of metachromatic dye (fluorochrome) is acridine orange. Under certain conditions it stains single-stranded ...
Hathaway WE, Newby LA, Githens JH (1964). "THE ACRIDINE ORANGE VIABILITY TEST APPLIED TO BONE MARROW CELLS. I. CORRELATION WITH ...
Darzynkiewicz Z, Kapuscinski J. (1990)"Acridine Orange, a Versatile Probe of Nucleic Acids and Other Cell Constituents." ... Another example of metachromatic dye (fluorochrome) is acridine orange. Under certain conditions it stains single-stranded ...
Cell apoptosis is tested by treating the lysosomal membrane with acridine orange. Acridine orange radiates a red fluorescent ...
Traganos F, Darzynkiewicz Z (1994). "Lysosomal proton pump activity: supravital cell staining with acridine orange ...
... consists of two acridine orange subunits that are bridged by a linear oxygenated spacer. Its fluorophore, and ... Ethidium bromide GelRed SYBR Green I Agarose gel electrophoresis and gel electrophoresis of nucleic acids Acridine orange US ... therefore its optical properties, are essentially identical to those of other N-alkylacridinium orange dyes. When exposed to ...
1977). Bacterial cells were counted with an epifluorescence microscope, producing what is called an "acridine orange direct ...
Several dyes have been studied for MUSE's application, including eosin, rhodamine, DAPI, Hoechst, acridine orange, propidium ...
The blood is taken in a QBC capillary tube which is coated with acridine orange (a fluorescent dye) and centrifuged; the ...
The blood is taken in a QBC capillary tube which is coated with acridine orange (a fluorescent dye) and centrifuged; the ...
Fluorescent dyes include DAPI, Acridine Orange, SYBR Green 1, and YO-PRO-1, all of which are capable of staining both DNA and ... Gonzalez K, Mcvey S, Cunnick J, Udovichenko IP, Takemoto DJ (1995). "Acridine orange differential staining of total DNA and RNA ... while synechococcus emits both orange and red fluorescence; orange from phycobilins and red from chlorophyll. Besides ...
M Garciafernandez; D Ceccarelli; U Muscatello (2004). "Use of the fluorescent dye 10-N-nonyl acridine orange in quantitative ... Jacobson J, Duchen MR, Heales SJ (2002). "Intracellular distribution of the fluorescent dye nonyl acridine orange responds to ... Keij JF, Bell-Prince C, Steinkamp JA (2000). "Staining of mitochondrial membranes with 10-nonyl acridine orange, MitoFluor ... Based on this special structure, the fluorescent mitochondrial indicator, nonyl acridine orange (NAO) was introduced in 1982, ...
Affected cells do not exhibit hallmarks of apoptosis but rather autophagy as seen by staining with acridine orange and ...
B. turicatae is best seen by dark-field microscopy, but the organisms can also be detected using acridine orange or Wright's ...
Apoptosis was evaluated by flow cytometry on PBMC from HIV infected individuals (cells were stained with acridine orange and ...
They applied high alternating voltages in air to materials such as acridine orange, either deposited on or dissolved in ...
... as revealed by acridine orange staining and PCR-based diagnoses". Tropical Medicine and International Health. 3 (4): 304-312. ...
... acridine orange stain, and phase-contrast microscopy. It is capable of forming biofilms and can convert from spiral to a ...
An exception is the metachromatic fluorochrome acridine orange, which under the specific staining protocol can differentially ... or DNA susceptibility to denaturation at low pH using the metachromatic dye acridine orange, reveals the G1Q, G1A, and G1B cell ... Aside from propidium iodide and acridine orange, quantifiable dyes that are frequently used include (but are not limited to) ...
... when Bernanose and coworkers first produced electroluminescence in crystalline thin films of acridine orange and quinacrine. In ...
... acridine orange MeSH D03.494.046.250.177 - acriflavine MeSH D03.494.046.250.200 - aminacrine MeSH D03.494.046.250.225 - ...
Further details can be found in the IARC Monographs Acenaphthene Acepyrene Aciclovir Acridine orange Acriflavinium chloride ... acridine Benz[c]acridine Benzo[g]chrysene Benzo[a]fluoranthene Benzo[ghi]fluoranthene Benzo[a]fluorene Benzo[b]fluorene Benzo[c ... Orange I Orange G Oxyphenbutazone Palygorskite (attapulgite) (short fibres, < 5 micrometers) Paracetamol (Acetaminophen) ... metallic Chrysene Chrysoidine CI Acid Orange 3 Cimetidine Cinnamyl anthranilate CI Pigment Red 3 Citrinin Clofibrate Clomiphene ...
Psoralens, coal tars, photo-active dyes (eosin, acridine orange) Musk ambrette, methylcoumarin, lemon oil (may be present in ... In these areas an unsightly orange to brown tint may form, usually near or on the face. Many medications and conditions can ...
The flow-cytometric assay utilizing the fluorochrome acridine orange shows that DNA fragmentation within individual cells is ...
Smears of the kidney, liver, and spleen can be stained with Gram, Giemsa, acridine orange, or methylene blue stain for direct ...
The molecular formula C17H19N3 (molar mass : 265.35 g/mol) may refer to: Acridine orange Antazoline Esmirtazapine Mirtazapine A ...
... and then in 1985 of the F plasmid by acridine orange curing.[citation needed] Strains derived from MG1655 include DH1, parent ...
... orange).[160] ... Acridine derivatives[104] Anthranilic acid [113] Rutacridone, ...
Further details can be found in the IARC Monographs Acenaphthene Acepyrene Aciclovir Acridine orange Acriflavinium chloride ... acridine Benz[c]acridine Benzo[g]chrysene Benzo[a]fluoranthene Benzo[ghi]fluoranthene Benzo[a]fluorene Benzo[b]fluorene Benzo[c ... Orange I Orange G Oxyphenbutazone Palygorskite (attapulgite) (short fibres, < 5 micrometers) Paracetamol (Acetaminophen) ... metallic Chrysene Chrysoidine CI Acid Orange 3 Cimetidine Cinnamyl anthranilate CI Pigment Red 3 Citrinin Clofibrate Clomiphene ...
Ferguson LR, Denny WA (September 1991). "The genetic toxicology of acridines". Mutation Research. 258 (2): 123-60. doi:10.1016/ ... Interaction of DNA (in orange) with histones (in blue). These proteins' basic amino acids bind to the acidic phosphate groups ... Most intercalators are aromatic and planar molecules; examples include ethidium bromide, acridines, daunomycin, and doxorubicin ... T7 RNA polymerase (blue) producing an mRNA (green) from a DNA template (orange)[93] ...
Acid orange 5. *Acid orange 7. *Acifluorfen. *Acifran. *Acridine yellow. *Acridone. *Acrinathrin ...
Traganos F, Darzynkiewicz Z.(1994) Lysosomal proton pump activity: supravital cell staining with acridine orange differentiates ...
... acridine orange stain, and phase-contrast microscopy. It is capable of forming biofilms[23] and can convert from spiral to a ...
It has the form of an orange or brown powder. It may be harmful in the eyes or if inhaled. It is a dye and it stains the skin ... It is derived from acridine. Commercial preparations are often mixtures with proflavine. It is known by a variety of commercial ...
... damages DNA and is used as a mutagen in microbiology. Acridine yellow is similar to acridine orange. Acridine ... Acridine yellow, also known as acridine yellow G, acridine yellow H107, basic yellow K, and 3,6-diamino-2,7-dimethylacridine, ... It is a derivate of acridine. In histology, it is used as a fluorescent stain, and as a fluorescent probe for non-invasive ...
Acridine derivatives are also G4 ligands and through fluorescence resonance energy transfer (FRET) melting assays, ... The ligands for i-motif used as fluorescent probes include Thiazole orange, 2,2'-diethyl-9-methylselenacarbocyanine bromide ( ... Tilorone and Tobramycin are i-motif binding ligands discovered via thiazole orange fluorescence intensity displacement (FID) ... diethylenetriamine (BisA) was determined to increase the melting temperature of both i-motif and G4, while monomeric acridine ( ...
... and related derivatives (such as amsacrine) bind to DNA and RNA due to their abilities to intercalate. Acridine orange ... biologically active acridines, applications of acridines, new syntheses and reactions of acridines] Media related to Acridine ... Acridine is easily oxidized by peroxymonosulfuric acid to the acridine amine oxide. The carbon 9-position of acridine is ... at one time acridine dyes were popular but they are now relegated to niche applications, such as with acridine orange. The name ...
Its reaction with benzenediazonium chloride gives the cationic azo dye Basic Orange 1. Condensation of 2,4-diaminotoluene with ... acetaldehyde gives the acridine dye called Basic Yellow 9. NIOSH Pocket Guide to Chemical Hazards. "#0620". National Institute ...
Acridine orange and fluorescein have a maximum excitation at 502nm and 525 nm (green). When acridine orange associates with RNA ... Acridine orange is useful in the rapid screening of ordinarily sterile specimens. When acridine orange is used with flow ... When the pH of the environment is 3.5, acridine orange becomes excited by blue light (460 nm). When acridine orange is excited ... using acridine orange is a method known for examining the microbial content within food and water. The use of acridine orange ...
Acridine orange has been used as a fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Acridine orange at a ... Acridine Orange hemi(zinc chloride) salt from Sigma-Aldrich,Application: ... Acridine orange; 10 mg/mL solution in water from AnaSpec. 2. Acridine orange 10-nonyl bromide from AnaSpec. 3. Acridine orange ... Acridine orange has been used as a fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Acridine orange at a ...
... acridine hydrochloride zinc chloride double salt (CAS 10127-02-3), a cell-permeable metachromatic fluorescent cationic DNA and ... Acridine orange for cell sorting of sputum.. J Histochem Cytochem 27:552-6 (1979). Read more (PubMed: 86576) » ... The use of thionin and acridine orange in staining semithin sections of plant material embedded in epoxy resin.. Stain Technol ... Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. ...
The use of acridine orange stained buffy coat smears was assessed as a rapid screening test for bacteremia in adults. A total ... Rapid Diagnosis of Bacteremia in Adults Using Acridine Orange Stained Buffy Coat Smears. Mark Miller1,2 and Jack Mendelson1 ... Compared to blood culture, the overall sensitivity of acridine orange stained buffy coat smears was 16%, specificity 88%, and ... from which a buffy coat smear was prepared and stained with acridine orange (100 mg/L; pH 3.0). Forty-one of 356 blood samples ...
Acridine Orange: A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and ... Acridine Orange; Diamine, Tetramethyl Acridine; Orange 3RN, Basic; Orange Base, Acridine; Orange, Acridine; Orange, Rhoduline; ... 3,6-Bis(dimethylamino)acridine; Acridine Orange Base; Basic Orange 3RN; C.I. 46005; C.I. Basic Orange 14; Rhoduline Orange; ... Acridine Orange. Subscribe to New Research on Acridine Orange A cationic cytochemical stain specific for cell nuclei, ...
... Joanne Lannigan joannelannigan at virginia.edu Mon Jun 7 15:20:40 EST 2004 *Previous message: Acridine Orange ... Acridine Orange , , Does anyone routinely run acridine orange through their , FACScan/FACSort? I would like suggestions for ...
... Does anyone routinely run acridine orange through their FACScan/FACSort? I would like suggestions for cleaning ... Acridine Orange. DARZYNKIEWICZ ZBIGNIEW Z_DARZYNKIEWICZ at nymc.edu Mon Jun 7 14:47:50 EST 2004 *Previous message: Parts to ... We do routinely use acridine orange using FACScan. A 20 min rinse with 10 times water-diluted Chlorox bleach is adequate to ...
Diagnosis of malaria by acridine orange fluorescent microscopy in an endemic area of venezuela.. Bosch I1, Bracho C, Pérez HA. ... Fluorescent (acridine orange) microscopical examination of capillary centrifuged blood (quantitative buffy coat [QBC] analysis ...
A grade of acridine orange of exceptionally high purity, suitable for quantitative work. Free of inorganic salts. ... Polysciences grade of acridine orange is of exceptionally high purity, suitable for quantitative work. It is free of inorganic ... Acridine orange is a nucleic acid-selective fluorescent cationic dye useful for cell cycle determination. It emits green ... We offer a highly purified form of acridine orange while most of the other commercially available grades are either of low ...
By Leo Chan,2018-06-20T20:27:59+00:00May 20th, 2015,Categories: Cellometer Application News,Tags: Acridine Orange, Propidium ...
Spermatozoa bound to solid state hyaluronic acid show chromatin structure with high DNA chain integrity: an acridine orange ... we tested the hypothesis that HA-bound sperm would be enhanced in sperm of high DNA chain integrity and green acridine orange ...
Comparison of the automicrobic system, acridine orange-stained smears, and gram-stained smears in detecting bacteriuria.. B A ... We compared the accuracy of the Gram-stained smear, the acridine orange-stained smear, and the AutoMicrobic system (AMS; Vitek ... For 1,024 clinical specimens, results with the acridine orange-stained smear and the Gram-stained smear were very similar. When ... Comparison of the automicrobic system, acridine orange-stained smears, and gram-stained smears in detecting bacteriuria. ...
Acridine orange staining was used to visualize apoptosis in A172 (A) and U87 (B). Cells were treated with 0, 10 or 100 n ... Acridine orange staining was used to visualize apoptosis in A172 (A) and U87 (B). Cells were treated with 0, 10 or 100 ng/ml ... Acridine orange staining was used to visualize apoptosis in A172 (A) and U87 (B). Cells were treated with 0, 10 or 100 ng/ml ... After 24h, cells were stained using acridine orange dye. Mentions: To confirm that the combinatorial effects described above ...
Acridine Orange, 10 mg/mL in Water (High Purity) (AO) Acridine orange (AO) stains dsDNA green (525 nm) and RNA or single ... Acridine orange (AO) stains dsDNA green (525 nm) and RNA or single stranded DNA red (650 nm). The dye is membrane-permeant and ... 3 Acridine orange has also been used for the detection of microorganisms in cerebrospinal fluid and other clinical specimens.We ... offer a highly purified form of acridine orange while most of the other commercially available grades of AO are either in zinc ...
Zhang, Y.; Pan, J.; Zhang, G.; Zhou, X. Intercalation of herbicide propyzamide into DNA using acridine orange as a fluorescence ... Silica nanoparticles assisted photodegradation of acridine orange in aqueous suspensions. Appl. Catal., B 2015, 168-169, 363- ... An RNA polymerase inhibitorAcridine Orange acts as a lysosomal dye. It is used for cell-cycle studies. It plays an important ...
Looking for abbreviations of AO/PI? It is Acridine Orange and Propidium Iodide. Acridine Orange and Propidium Iodide listed as ... Acridine Orange and Propidium Iodide - How is Acridine Orange and Propidium Iodide abbreviated? https://acronyms. ... a href=https://acronyms.thefreedictionary.com/Acridine+Orange+and+Propidium+Iodide,AO/PI,/a,. *Facebook ... redirected from Acridine Orange and Propidium Iodide) Acronym. Definition. AO/PI. Acridine Orange and Propidium Iodide. ...
The study revealed that the Acridine technique was at least as sensitive as the older Giemsa technique, but proved quicker and ... The differentiation of Wuchereria banerofti and Dipetalonema perstans microfilariae when stained with Acridine is discussed. ... The present study compares the value of the Acridine Orange technique and the Giemsa staining technique for the detection of ... oa Central African Journal of Medicine - The use of acridine orange 0 in the laboratory diagnosis of human filariasis * ...
With this concept, we have conjugated the acridine orange part to a bombesin peptide directly on the (99m)Tc core and in one ... With this concept, we have conjugated the acridine orange part to a bombesin peptide directly on the (99m)Tc core and in one ... We have synthesized acridine orange derivatives which comprise an imidazole-2-carbaldehyde function for coordination to the Re( ... We have synthesized acridine orange derivatives which comprise an imidazole-2-carbaldehyde function for coordination to the Re( ...
"Utility of Acridine Orange staining for detection of bacteria from positive blood cultures, Journal Of Microbiological Methods ... Utility of Acridine Orange staining for detection of bacteria from positive blood cultures. Utility of Acridine Orange staining ... Utility of Acridine Orange staining for detection of bacteria from positive blood cultures. Neeraja, M.; Lakshmi, V.; Padmasri ... Evaluation of acridine orange staining as a replacement of subcultures for BacT/ALERT-positive, Gram stain-negative blood ...
Metachromasy in clay dye systems; the adsorption of acridine orange by Na-beidellite D. Garfinkel-Shweky D. Garfinkel-Shweky ... D. Garfinkel-Shweky, S. Yariv; Metachromasy in clay dye systems; the adsorption of acridine orange by Na-beidellite. Clay ... The adsorption of the metachromic dye acridine orange (AO) by Na-beidellite was investigated by visible spectroscopy. Different ... Metachromasy in clay-dye systems; the adsorption of acridine orange by Na-saponite Clay Minerals ...
Acridine orange[edit]. Acridine orange (AO) is a nucleic acid selective fluorescent cationic dye useful for cell cycle ... The stain may also be used in conjunction with acridine orange (AO) in viable cell counting. This EB/AO combined stain causes ... It uses a combination of haematoxylin, Orange G, eosin Y, Light Green SF yellowish, and sometimes Bismarck Brown Y. ... In a skilfully made H & E preparation the red blood cells are almost orange, and collagen and cytoplasm (especially muscle) ...
Vital staining with the dye, acridine orange, has been previously used to analyze patterns of apoptosis in both vertebrate and ... Visualization of Programmed Cell Death in Whole Embryos Using Acridine Orange. Thu, 12 Jul 2012 , Situ Hybridization ... cells fragment into a number of apoptotic bodies that are chromatin-rich and are intensely stained by acridine orange. In ...
Will try new PBS first, then a concentration gradient of acridine orange. ... Acridine orange. Fri, 08/03/2012 - 20:01 - kdorfman Sigma A8097-10 mL ...
BioTek Product Accessory, Part Number: 1225109, for use with Lionheart FX Microscope automatisé , Cytation 7 Cell Imaging Multi-Mode Reader, Cytation 5 Cell Imaging Multi-Mode Reader, Lionheart LX Microscope automatisé and Cytation 1 Cell Imaging Multi-Mode Reader
Discover things that you didnt know about acridine orange on echemi.com. ... You can get all kinds of articles on acridine orange here. ... acridine orange sds. acridine orange base. acridine orange ... acridin orange. acridine orange emission. acridine orange apoptosis. acridine orange solubility. ... acridine orange staining. ispa orange. sypro orange. monsanto agent orange. agent orange effects on offspring. ...
... acridine explanation free. What is acridine? Meaning of acridine medical term. What does acridine mean? ... Looking for online definition of acridine in the Medical Dictionary? ... Acridine orange stains nucleic acid so that under ultraviolet light DNA appears green and RNA appears orange.. acridine (orange ... acridine. Also found in: Dictionary, Encyclopedia, Wikipedia.. Related to acridine: acridine dyes anthracene. [an´thrah-sēn] a ...
Supplier and Exporter of ACRIDINE ORANGE FOR MICROSCOPICAL STAINING based in Mumbai, India and We Export in Brazil, Argentina, ...
Acridine Orange, Acridine Orange for Staining which are used for various applications including medical uses, pharmaceuticals ... exporter of all laboratory chemicals including Acridine Orange for Microscopical Staining, ... Technical Specifications of Acridine Orange for Microscopical Staining :-. Zinc chloride double salt for electrophoresis ... This is done during the excitation of Acridine Orange under blue light at pH level of 3.5 while detecting the cells with the ...
Acridine Orange/Di-8-ANEPPS , AT Series 单波段带通 , Included Filters: AT480/30x, AT505DC, AT620/60m , Related Fluorochromes: ... Acridine Orange + RNA, Di-8-ANEPPS non-ratiometric, Pulsar™ 650, DiA, FM™ 1-43, Ethidium Bromide , ...
Acridine Orange Staining. A549 cells (1 × 106 per well) were treated with Ori (20 μM) and CQ (50 μM) for 18 h and incubated ... From the results that P62 and LC3 were all overexpressed in Ori-treated cells, we added CQ (50 μM) through acridine orange ... were stained with acridine orange. Scale bars: 10 μM. (K-N) Cells were treated with Ori (20 μM) in the absence or presence of ... with acridine orange solution for 15 min and images were collected by fluorescence microscope. ...
  • In this work, we tested the hypothesis that HA-bound sperm would be enhanced in sperm of high DNA chain integrity and green acridine orange fluorescence (AOF) compared with the original sperm in semen. (nih.gov)
  • Zhou, X. Intercalation of herbicide propyzamide into DNA using acridine orange as a fluorescence probe. (alfa.com)
  • This is done during the excitation of Acridine Orange under blue light at pH level of 3.5 while detecting the cells with the help of fluorescence microscope. (alphachemikaindia.com)
  • Since they are displaced by the float to the easily inspected twenty-nine micron free space surrounding the float, they can be visualized, by placing the centrifuged tube in a notched holder and examining the tube using a 50X oil objective on an epi-illuminating microscope, due to the parasites' uptake of acridine orange and the resultant fluorescence (14) (Figures 2 and 3). (thefreedictionary.com)
  • Reliability of acridine orange fluorescence microscopy in oral cytodiagnosis. (bvsalud.org)
  • The present study was conducted to determine the reliability of acridine orange fluorescence microscopy for cytodiagnosis as a more rapid and easier method for the final evaluation of the cytological specimen. (bvsalud.org)
  • The acridine orange fluorescence stain reliably demonstrated malignant cells based on the differential fluorescence - a cytochemical criterion. (bvsalud.org)
  • However, the acridine orange fluorescence stain did not differentiate effectively between malignant cells and rapidly proliferating cells, as the technique is based on the nucleic acid content. (bvsalud.org)
  • Acridine yellow, also known as acridine yellow G, acridine yellow H107, basic yellow K, and 3,6-diamino-2,7-dimethylacridine, is a yellow dye with strong bluish-green fluorescence. (wikipedia.org)
  • Fluorescence image of 15 zebrafish larvae (fixed and stained with acridine orange) within the field of the Mesolens. (coolled.com)
  • When acridine orange is excited by blue light, the fluorescent dye can differentially stain human cells green and prokaryotic cells orange (600 nm), allowing for rapid detection with a fluorescent microscope. (wikipedia.org)
  • Past and present studies comparing acridine orange staining with blind subcultures for the detection of positive blood cultures showed that the acridine orange is a simple, inexpensive, rapid staining procedure that appeares to be more sensitive than the Gram stain for detecting microorganisms in cerebrospinal fluid and other clinical and non-clinical materials. (wikipedia.org)
  • When acridine orange is used with flow cytometry, the differential stain is used to measure DNA denaturation and the cellular content of DNA versus RNA in individual cells, or detect DNA damage in infertile sperm cells. (wikipedia.org)
  • Acridine orange staining has to be performed at an acidic pH to obtain the differential staining, which allows bacterial cells to stain orange and tissue components to stain yellow or green. (wikipedia.org)
  • Application: Acridine orange has been used as a fluorescent stain for nucleic acids in agarose and polyacrylamide gels. (bio-medicine.org)
  • The sensitivity of Gram stain (GS) was 98.26% while Acridine Orange (AO) stain proved to be more sensitive (100%) with a Positive and Negative Predictive Value of 100% each. (deepdyve.com)
  • IMSEAR at SEARO: A modified thionin acridine-orange stain for mast cells. (who.int)
  • One smear was stained with Papanicolaou stain and another one with acridine orange stain. (bvsalud.org)
  • Acridine Orange stain intercalates into nucleic acids to enhance visibility of nuclei in clusters of cells (Figure 3). (thermofisher.com)
  • RNA was isolated from consecutive tissue sections that were either unstained or stained with three different dyes (Ambion's Cresyl Violet and Acridine Orange, and a Competitor Stain). (thermofisher.com)
  • Acridine dyes are prepared via the condensation of 1,3-diaminobenzene with suitable benzaldehydes. (wikipedia.org)
  • By using the process of condensation, Acridine dyes can be prepared by condensing 1,3-diaminobenzene and allied benzaldehydes. (alphachemikaindia.com)
  • Acridine orange (AO) and propidium iodide (PI) are nucleic acid binding dyes that can be used to measure the cell viability. (nexcelom.com)
  • Isolated cells stained with Acridine Orange will fluoresce on the cap after microdissection, making it easy to assess whether the cells were effectively microdissected and retrieved. (thermofisher.com)
  • The use of thionin and acridine orange in staining semithin sections of plant material embedded in epoxy resin. (abcam.com)
  • We have tried thionin and acridine orange stains and found it to be good as it showed good colour contrast between mast cells and the background tissue. (who.int)
  • The differential staining capability of acridine orange provides quick scanning of specimen smears at lower magnifications of 400x compared to Gram stains that operate at 1000x magnification. (wikipedia.org)
  • Acridine orange is recommended for the use of fluorescent microscopic detection of microorganisms in smears prepared from clinical and non-clinical materials. (wikipedia.org)
  • The use of acridine orange stained buffy coat smears was assessed as a rapid screening test for bacteremia in adults. (hindawi.com)
  • Direct staining of blood smears with acridine orange and enumeration using fluorescent microscopy allowed quantitation of Haemophilus influenzae in blood at densities from 10(5) to 10(8) cfu/ml. (curehunter.com)
  • Fluorescent (acridine orange) microscopical examination of capillary centrifuged blood (quantitative buffy coat [QBC] analysis) and Giemsa stained thick blood smears (GTS) were compared for diagnosis of malaria in blood specimens from adults living in malaria transmission areas of the States of Bolivar and Amazonas in southeastern and south Venezuela, respectively. (nih.gov)
  • Comparison of the automicrobic system, acridine orange-stained smears, and gram-stained smears in detecting bacteriuria. (asm.org)
  • Acridine orange/ethidium bromide (AO/EtBr) and Hoechst 33342 were obtained from Fluka (Ronkonkoma, NY, USA). (thefreedictionary.com)
  • In addition, typical morphologic changes were observed in the cells with acridine orange/ethidium bromide staining. (mdpi.com)
  • A wide range of nucleic acid stains for polyacrylamide and agarose are available including Nancy-520, Ethidium bromide, SYBR ® Green I and II, methylene blue, DAPI, acridine orange and others. (sigmaaldrich.com)
  • Morphological changes were observed after staining cells with ethidium bromide/acridine orange (EB/AO) and Giemsa dye. (biomedcentral.com)
  • Autophagy were assessed by acridine orange staining, MDC staining and Transmission electron microscopy. (nih.gov)
  • Autophagic cell death induced by SAHA was confirmed by acridine orange staining and transmission electron microscopy (TEM) in TAMR/MCF-7 cells. (medsci.org)
  • We offer a highly purified form of acridine orange while most of the other commercially available grades are either of low purity or in zinc chloride complex form. (polysciences.com)
  • Acridine orange is an organic compound that serves as a nucleic acid-selective fluorescent dye with cationic properties useful for cell cycle determination. (wikipedia.org)
  • Acridine orange is a nucleic acid-selective fluorescent cationic dye useful for cell cycle determination. (polysciences.com)
  • DAPI, Acridine Orange, and Feulgen staining). (carleton.edu)
  • Acridine orange (AO) stains dsDNA green (525 nm) and RNA or single stranded DNA red (650 nm).The dye is membrane-permeant and its nucleic acid binding property has been used for cell-cycle studies. (biotium.com)
  • Acridine orange stains nucleic acid so that under ultraviolet light DNA appears green and RNA appears orange. (thefreedictionary.com)
  • At least for ex vivo biopsy analysis, however, using exogenous nuclear stains, such as acridine orange, could largely circumvent this problem. (thefreedictionary.com)
  • The staining efficiency of the fluorescent stains ranged from 80.33 ± 2.99 (Sytox Green) to 100% (Acridine Orange) for non-viable eggs. (scirp.org)
  • Acridine orange is able to withstand low pH environments, allowing the fluorescent dye to penetrate acidic organelles such as lysosomes and phagolysosomes that are membrane-bound organelles essential for acid hydrolysis or for producing products of phagocytosis of apoptotic cells. (wikipedia.org)
  • The ability to penetrate the cell membranes of acidic organelles and cationic properties of acridine orange allows the dye to differentiate between various types of cells (i.e., bacterial cells and white blood cells). (wikipedia.org)
  • Acridine orange staining was used to visualize apoptosis in A172 (A) and U87 (B). Cells were treated with 0, 10 or 100 ng/ml rhTRAIL WT and 0, 25 or 50 μM DMC. (nih.gov)
  • After 24h, cells were stained using acridine orange dye. (nih.gov)
  • During apoptosis, cells fragment into a number of apoptotic bodies that are chromatin-rich and are intensely stained by acridine orange. (alpfmedical.info)
  • The shape and structure of the petals are similar to among the leaves and are made up of thin-walled cells that can contain chromosomes that make the petals yellow, orange, orange or red. (echemi.com)
  • Moreover, it helps in staining human cells with and prokaryotes with green and bright orange color, respectively. (alphachemikaindia.com)
  • Bafilomycin A1 (Sigma Chemical Co., St. Louis, MO) was dissolved in DMSO and added to the cells 30 min before addition of acridine orange. (aacrjournals.org)
  • Liapunova E.A., Zelenin A.V., The effect of acridine orange on the early steps of protein biosynthesis in tissue culture cells, Voprosy meditsinskoi khimii, 1966, vol: 12(2), 218-220. (msk.ru)
  • Cells were then stained with acridine orange 1: Control group. (nih.gov)
  • VectaCell Acridine Orange and VectCell Rhodamine 123 reagents offer convenience and ease of use for visualizing different cellular components in live cells. (vectorlabs.com)
  • f Acridine orange marks few dying cells in control eye-antennal disc. (nature.com)
  • g , h Number of acridine orange positive cells were enhanced upon mahe overexpression in dosage sensitive manner. (nature.com)
  • The two micrographs (right) show live mononuclear cells stained with acridine orange in green, and dead cells stained with PI in red. (nexcelom.com)
  • Another example of metachromatic dye (fluorochrome) is acridine orange. (wikipedia.org)
  • This unit presents two protocols for differential staining, one using the metachromatic dye acridine orange (AO) and the other a combination of pyronin Y (PY) and Hoechst 33342. (currentprotocols.com)
  • Giemsa staining, acridine orange (AO) staining and semi-nested multiplex PCR. (curehunter.com)
  • Cell viability was measured by MTS assay and apoptosis was assessed by Annexin V/PI and acridine orange staining. (nih.gov)
  • The present study compares the value of the Acridine Orange technique and the Giemsa staining technique for the detection of Dipetalonema perstans in blood films. (journals.co.za)
  • Vital staining with the dye, acridine orange, has been previously used to analyze patterns of apoptosis in both vertebrate and invertebrate embryos (3,4). (alpfmedical.info)
  • The effect of varicocele on sperm morphology and DNA maturity: does acridine orange staining facilitate diagnosis? (thefreedictionary.com)
  • Evaluation of sperm nuclear DNA normality by acridine orange staining technique. (bvsalud.org)
  • Semen samples were assessed for conventional parameters (volume, percentage of normal morphology, percentage of progressive motility, sperm concentration, and round cell concentration) according to World Health Organization ( WHO ) guideline and acridine orange staining . (bvsalud.org)
  • Therefore, the acridine orange staining technique for evaluation of the sperm DNA normality appears to give more information in infertile patients with normal semen analysis . (bvsalud.org)
  • Acridine orange staining is a methodology for nucleic acid selective metachromatic staining that is useful for following cell cycle determination. (unomaha.edu)
  • This work presents methodology and results of a cell staining that utilizes acridine orange fluoresence. (unomaha.edu)
  • Staining with the lysosomotropic agent acridine orange enabled us to quantify AVO accumulation and to demonstrate their time- and dose-dependent appearance. (aacrjournals.org)
  • Supravital Cell-staining with Acridine Orange. (aacrjournals.org)
  • ClC-3 overexpression increased the acidity of intracellular vesicles, as assessed by acridine orange staining, and enhanced resistance to the chemotherapeutic drug etoposide by almost doubling the IC 50 in either BON or HEK293 cell lines. (aacrjournals.org)
  • By acridine orange staining. (cdc.gov)
  • Acridine orange staining and MDC staining showed that Interferon-λ1 triggered the accumulation of acidic vesicular and autolysosomes in osteosarcoma cell. (nih.gov)
  • LCM Sample Stained with Acridine Orange in Ambion's LCM Staining Kit. (thermofisher.com)
  • As seen in the electropherogram trace, a good 28S/18S rRNA ratio is maintained with human tumor samples stained with Cresyl Violet or Acridine Orange in the LCM Staining Kit (Figure 4). (thermofisher.com)
  • f - j Acridine orange staining of eye-antennal imaginal discs. (nature.com)
  • Characterization of cultures utilizing acridine orange staining procedures. (currentprotocols.com)
  • Here we review a common method for measuring cell viability: Acridine Orange/Propidium Iodide. (nexcelom.com)
  • The study revealed that the Acridine technique was at least as sensitive as the older Giemsa technique, but proved quicker and less tiring to use. (journals.co.za)
  • Diagnosis of malaria by acridine orange fluorescent microscopy in an endemic area of venezuela. (nih.gov)
  • Acridine orange has been widely accepted and used in many different areas, such as epifluorescence microscopy, and the assessment of sperm chromatin quality. (wikipedia.org)
  • Acridine orange (AO) helps identify lysosomes and other intracellular organelles. (sigmaaldrich.com)
  • Polysciences' grade of acridine orange is of exceptionally high purity, suitable for quantitative work. (polysciences.com)
  • Acridine orange (Polysciences, Warrington, PA) was added at a final concentration of 1 μg/ml for a period of 15 min. (aacrjournals.org)
  • Amac: Bu calismanin amact varikoseli olan hastalarda sperm deoksiribontikleik asit (DNA) fragmantasyonunun saptanmast icin rutin ofis prosedurlerine ilaveten Acridine Orange (AO) kullaniminin arastirilmasidtr. (thefreedictionary.com)
  • Varikoselin sperm morfolojisi ve DNA maturitesi uzerine etkisi: acridine orange boyama taniyi kolaylastirir mi? (thefreedictionary.com)
  • Acridine orange is used in epifluorescence microscopy and flow cytometry. (wikipedia.org)
  • Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. (abcam.com)
  • Acridine has antimicrobial factors useful in drug-resistant bacteria and isolating bacteria in various environments. (wikipedia.org)
  • Acridine orange in the mid-twentieth century was used to examine the microbial content found in soil and direct counts of aquatic bacteria. (wikipedia.org)
  • Additionally, the method of acridine orange direct count (AODC) proved useful in the enumeration of bacteria found within landfills. (wikipedia.org)
  • The use of acridine orange in clinical applications has become widely accepted, mainly focusing on highlighting bacteria in blood cultures. (wikipedia.org)
  • Mycobacterium tuberculosis in a sputum smear is stained using fluorescent auramine with acridine orange counterstain. (polysciences.com)
  • For 1,024 clinical specimens, results with the acridine orange-stained smear and the Gram-stained smear were very similar. (asm.org)
  • Acridine orange and fluorescein have a maximum excitation at 502nm and 525 nm (green). (wikipedia.org)
  • When acridine orange associates with RNA, the fluorescent dye experiences a maximum excitation shift from 525 nm (green) to 460 nm (blue). (wikipedia.org)
  • When acridine orange binds to DNA, the dye exhibits a maximum excitation at 502 nm producing a maximum emission of 525 nm. (wikipedia.org)
  • When bound to RNA, acridine orange displays a maximum emission value of 650 nm and a maximum excitation value of 460 nm. (wikipedia.org)
  • The maximum excitation and emission value that occur when acridine orange is bound to RNA are the result of electrostatic interactions and the intercalation between the acridine molecule and nucleic acid-base pairs present within RNA and DNA. (wikipedia.org)
  • We do routinely use acridine orange using FACScan. (purdue.edu)
  • creswelk at georgetown.edu ] Sent: Friday, June 04, 2004 1:17 PM To: cyto-inbox Subject: Acridine Orange Does anyone routinely run acridine orange through their FACScan/FACSort? (purdue.edu)
  • Acridine orange is derived from the organic molecule acridine, which was first discovered by Carl Grabe and Heinrich Caro, who isolated acridine by boiling coal in Germany during the late nineteenth century. (wikipedia.org)
  • Acridine yellow damages DNA and is used as a mutagen in microbiology. (wikipedia.org)
  • Acridine orange is useful in the rapid screening of ordinarily sterile specimens. (wikipedia.org)
  • The interaction of acridine orange with target molecules (i.e. (unomaha.edu)
  • The micronucleus assay with mouse peripheral blood reticulocytes using acridine orange-coated slides. (thefreedictionary.com)
  • We have synthesized acridine orange derivatives which comprise an imidazole-2-carbaldehyde function for coordination to the Re(CO)(3)](+) or (99)mTc(CO)(3)](+) core. (uzh.ch)
  • Derivatives of acridine are successful as antibacterial agents, finding their principal use as local antiseptics. (thefreedictionary.com)
  • Acridine orange is cell-permeable, which allows the dye to interact with DNA by intercalation, or RNA via electrostatic attractions. (wikipedia.org)
  • Non-toxic levels of neutral red and acridine orange were suitable tracers: they were visible in the stylet and gut after feeding for 24 h, and may also attract aphids to feed. (mdpi.com)
  • When bound to DNA, acridine orange is very similar spectrally to an organic compound known as fluorescein. (wikipedia.org)
  • Latterini, L. Silica nanoparticles assisted photodegradation of acridine orange in aqueous suspensions. (alfa.com)
  • Acridine orange at a concentration of 120 μM will detect purified DNA in gels with a sensitivity of 25-50 ng per band. (bio-medicine.org)
  • Will try new PBS first, then a concentration gradient of acridine orange. (umass.edu)
  • If tissue morphology is slightly damaged, Acridine Orange may also provide better contrast than Cresyl Violet. (thermofisher.com)
  • Aggregated AO fluoreses orange rather than green thus clearly differentiating the lysosomes from the other organelles. (sigmaaldrich.com)
  • Acridine orange is derived from dimethylaminobenzaldehyde and N,N-dimethyl-1,3-diaminobenzene. (wikipedia.org)