Acridine Orange: A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.AcridinesCitrus sinensis: A plant species of the genus CITRUS, family RUTACEAE that provides the familiar orange fruit which is also a source of orange oil.Aminoacridines: Acridines which are substituted in any position by one or more amino groups or substituted amino groups.Staining and Labeling: The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.Fluorescent Dyes: Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.Ethidium: A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide.Intercalating Agents: Agents that are capable of inserting themselves between the successive bases in DNA, thus kinking, uncoiling or otherwise deforming it and therefore preventing its proper functioning. They are used in the study of DNA.Azure Stains: PHENOTHIAZINES with an amino group at the 3-position that are green crystals or powder. They are used as biological stains.Lactose Factors: Plasmids which determine the ability of a bacterium to ferment lactose.Microscopy, Fluorescence: Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.Gentian Violet: A dye that is a mixture of violet rosanilinis with antibacterial, antifungal, and anthelmintic properties.Citrus: A plant genus of the family RUTACEAE. They bear the familiar citrus fruits including oranges, grapefruit, lemons, and limes. There are many hybrids which makes the nomenclature confusing.Coloring Agents: Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.Cardiolipins: Acidic phospholipids composed of two molecules of phosphatidic acid covalently linked to a molecule of glycerol. They occur primarily in mitochondrial inner membranes and in bacterial plasma membranes. They are the main antigenic components of the Wassermann-type antigen that is used in nontreponemal SYPHILIS SERODIAGNOSIS.Lysosomes: A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)PhenazinesBenzophenoneidum: An aniline dye used as a disinfectant and an antiseptic agent. It is weakly fluorescing and binds specifically to certain proteins.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Aminacrine: A highly fluorescent anti-infective dye used clinically as a topical antiseptic and experimentally as a mutagen, due to its interaction with DNA. It is also used as an intracellular pH indicator.Histiocytoma: A neoplasm containing HISTIOCYTES. Important forms include BENIGN FIBROUS HISTIOCYTOMA; and MALIGNANT FIBROUS HISTIOCYTOMA.Fluorophotometry: Measurement of light given off by fluorescein in order to assess the integrity of various ocular barriers. The method is used to investigate the blood-aqueous barrier, blood-retinal barrier, aqueous flow measurements, corneal endothelial permeability, and tear flow dynamics.Zinc Compounds: Inorganic compounds that contain zinc as an integral part of the molecule.Chlorides: Inorganic compounds derived from hydrochloric acid that contain the Cl- ion.Hexachlorophene: A chlorinated bisphenol antiseptic with a bacteriostatic action against Gram-positive organisms, but much less effective against Gram-negative organisms. It is mainly used in soaps and creams and is an ingredient of various preparations used for skin disorders. (From Martindale, The Extra Pharmacopoeia, 30th ed, p797)Zinc: A metallic element of atomic number 30 and atomic weight 65.38. It is a necessary trace element in the diet, forming an essential part of many enzymes, and playing an important role in protein synthesis and in cell division. Zinc deficiency is associated with ANEMIA, short stature, HYPOGONADISM, impaired WOUND HEALING, and geophagia. It is known by the symbol Zn.Cell Nucleus: Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Mutagens: Chemical agents that increase the rate of genetic mutation by interfering with the function of nucleic acids. A clastogen is a specific mutagen that causes breaks in chromosomes.District of Columbia: A federal area located between Maryland and Virginia on the Potomac river; it is coextensive with Washington, D.C., which is the capital of the United States.Minicomputers: Small computers that lack the speed, memory capacity, and instructional capability of the full-size computer but usually retain its programmable flexibility. They are larger, faster, and more flexible, powerful, and expensive than microcomputers.Guyana: A republic in the north of South America, east of VENEZUELA and west of SURINAME. Its capital is Georgetown.Myanmar: A republic of southeast Asia, northwest of Thailand, long familiar as Burma. Its capital is Yangon, formerly Rangoon. Inhabited by people of Mongolian stock and probably of Tibetan origin, by the 3d century A.D. it was settled by Hindus. The modern Burmese state was founded in the 18th century but was in conflict with the British during the 19th century. Made a crown colony of Great Britain in 1937, it was granted independence in 1947. In 1989 it became Myanmar. The name comes from myanma, meaning the strong, as applied to the Burmese people themselves. (From Webster's New Geographical Dictionary, 1988, p192 & Room, Brewer's Dictionary of Names, 1992, p367)Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Salts: Substances produced from the reaction between acids and bases; compounds consisting of a metal (positive) and nonmetal (negative) radical. (Grant & Hackh's Chemical Dictionary, 5th ed)Zona Pellucida: A tough transparent membrane surrounding the OVUM. It is penetrated by the sperm during FERTILIZATION.Hyaluronic Acid: A natural high-viscosity mucopolysaccharide with alternating beta (1-3) glucuronide and beta (1-4) glucosaminidic bonds. It is found in the UMBILICAL CORD, in VITREOUS BODY and in SYNOVIAL FLUID. A high urinary level is found in PROGERIA.Spermatozoa: Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.Sperm-Ovum Interactions: Interactive processes between the oocyte (OVUM) and the sperm (SPERMATOZOA) including sperm adhesion, ACROSOME REACTION, sperm penetration of the ZONA PELLUCIDA, and events leading to FERTILIZATION.Chromatin: The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.Dictionaries, MedicalDictionaries as Topic: Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.Dictionaries, ChemicalTerminology as Topic: The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.Bentonite: A colloidal, hydrated aluminum silicate that swells 12 times its dry size when added to water.Aluminum Silicates: Any of the numerous types of clay which contain varying proportions of Al2O3 and SiO2. They are made synthetically by heating aluminum fluoride at 1000-2000 degrees C with silica and water vapor. (From Hawley's Condensed Chemical Dictionary, 11th ed)Adsorption: The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.Photoelectron Spectroscopy: The study of the energy of electrons ejected from matter by the photoelectric effect, i.e., as a direct result of absorption of energy from electromagnetic radiation. As the energies of the electrons are characteristic of a specific element, the measurement of the energy of these electrons is a technique used to determine the chemical composition of surfaces.Kaolin: The most common mineral of a group of hydrated aluminum silicates, approximately H2Al2Si2O8-H2O. It is prepared for pharmaceutical and medicinal purposes by levigating with water to remove sand, etc. (From Merck Index, 11th ed) The name is derived from Kao-ling (Chinese: "high ridge"), the original site. (From Grant & Hackh's Chemical Dictionary, 5th ed)Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Glass: Hard, amorphous, brittle, inorganic, usually transparent, polymerous silicate of basic oxides, usually potassium or sodium. It is used in the form of hard sheets, vessels, tubing, fibers, ceramics, beads, etc.Fixatives: Agents employed in the preparation of histologic or pathologic specimens for the purpose of maintaining the existing form and structure of all of the constituent elements. Great numbers of different agents are used; some are also decalcifying and hardening agents. They must quickly kill and coagulate living tissue.Microtomy: The technique of using a microtome to cut thin or ultrathin sections of tissues embedded in a supporting substance. The microtome is an instrument that hold a steel, glass or diamond knife in clamps at an angle to the blocks of prepared tissues, which it cuts in sections of equal thickness.Waxes: A plastic substance deposited by insects or obtained from plants. Waxes are esters of various fatty acids with higher, usually monohydric alcohols. The wax of pharmacy is principally yellow wax (beeswax), the material of which honeycomb is made. It consists chiefly of cerotic acid and myricin and is used in making ointments, cerates, etc. (Dorland, 27th ed)Formaldehyde: A highly reactive aldehyde gas formed by oxidation or incomplete combustion of hydrocarbons. In solution, it has a wide range of uses: in the manufacture of resins and textiles, as a disinfectant, and as a laboratory fixative or preservative. Formaldehyde solution (formalin) is considered a hazardous compound, and its vapor toxic. (From Reynolds, Martindale The Extra Pharmacopoeia, 30th ed, p717)Picrates

Comparison of five methods of malaria detection in the outpatient setting. (1/413)

In eastern Africa where 90% of the malaria is due to Plasmodium falciparum, the accuracy of malaria diagnosis at the outpatient level is becoming increasingly important due to problems of drug resistance and use of alternative, costly antimalarial drugs. The quantitative buffy coat (QBC) technique, acridine orange staining with an interference filter system, and the ParaSight-F test have been introduced as alternative methods to conventional microscopy for the diagnosis of malaria. Two hundred thirteen outpatients were tested using these alternative methods and conventional microscopy by five experienced technologists; two were randomly allocated to read the results of each test. Paired results showed the highest level of agreement with the ParaSight-F test (99%), followed by Field stain (92%). The results of the QBC technique showed the least agreement (73%). Using conventional microscopy as the reference standard, the ParaSight-F test had a sensitivity range of 90-92% and a specificity of 99%, staining with acridine orange had a sensitivity range of 77-96% and a specificity range of 81-98% and the QBC technique had a sensitivity range of 88-98% and a specificity range of 58-90%. All microscopic tests showed lower sensitivities (as low as 20% using staining with acridine orange) in detecting low parasitemias (< or = 320/microl) than the ParaSight-F test (70%). Due to the high cost of the ParaSight-F test, Field-stained blood films remain the most appropriate method for diagnosis of P. falciparum in eastern Africa. The ParaSight-F test may be used in situations where no trained microscopists are available, or where malaria is strongly suspected and the results of microscopy are negative.  (+info)

Destruction of protamine in human sperm inhibits sperm binding and penetration in the zona-free hamster penetration test but increases sperm head decondensation and male pronuclear formation in the hamster-ICSI assay. (2/413)

PURPOSE: Our purpose was to investigate the fertilizing ability of human protamine-damaged sperm in a heterologous system using hamster oocytes. METHODS: The protamine of the sperm were damaged by exposure to dithiothreitol, a disulfide-reducing agent. Their ability to penetrate and form male pronuclei were investigated using the zona-free hamster penetration test and the hamster-intracytoplasmic sperm injection assay, respectively. RESULTS: The zona-free hamster penetration test revealed that protamine-damaged sperm are unable to bind and penetrate the hamster oocyte. On the other hand, hamster-intracytoplasmic sperm injection assay results showed that 56.9% and 39.2% of the injected oocytes developed male pronuclei in protamine-damaged and live-intact sperm groups, respectively, with a significant difference in these rates (P < 0.01). CONCLUSIONS: This study shows that protamine-damaged sperm are able to undergo sperm head decondensation and male pronuclear formation only when injected into the ooplasm, although they cannot bind and penetrate through the zona and enter the ooplasm.  (+info)

Reactive oxygen species-induced apoptosis and necrosis in bovine corneal endothelial cells. (3/413)

PURPOSE: The loss of corneal endothelial cells associated with aging and possibly other causes has been speculated to be related to exposure to reactive oxygen species (ROS). The current study was conducted to investigate, by use of photosensitizers, the underlying mechanisms involved in the death of bovine corneal endothelial cells (BCENs) caused by ROS. METHODS: BCEN cells in primary culture were treated with a photosensitizer (riboflavin or rose bengal) with light exposure. The patterns of cell damage and death were assessed using an acridine orange-ethidium bromide differential staining method, TdT-mediated dUTP nick-end labeling (TUNEL) assay, and transmission electron microscopy. The cytotoxicity was assayed by mitochondrial function using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) testing. Antioxidants, including catalase, L-histidine, salicylic acid, and superoxide dismutase, were used to determine the types of ROS involved. Activation of nuclear factor (NF)-kappaB was examined by fluorescent immunocytochemistry with anti-p65 antibody. RESULTS: Light-irradiated riboflavin or rose bengal resulted in a significant decrease in viability of BCEN cells. Chromosomal condensation and fragmentation were observed in apoptotic cells, and membrane lysis and damage of cell ultrastructures were observed in necrotic cells. Riboflavin induced apoptosis at 30 minutes and thereafter and induced necrosis after 2 hours. Rose bengal was shown to cause similar effects within half the time required for the effects of riboflavin. Catalase and salicylic acid were found to provide protection for BCENs from cytotoxic effects of riboflavin, and L-histidine was found to protect BCENs from cytotoxicity induced by rose bengal. Kinetic studies using immunocytochemistry showed that NF-kappaB was translocated into the nucleus within 15 minutes and 30 minutes after treatment with rose bengal and riboflavin, respectively. CONCLUSIONS: The cytotoxic effects of photo-irradiated riboflavin and rose bengal are shown to be mediated by two distinct but parallel pathways, one leading to apoptosis and the other to necrosis. Possible involvement of NF-kappaB in cell death is suggested. These findings provide potential leads for future investigation into the molecular mechanisms of loss of corneal endothelial cells related to aging, oxidative stress, and possibly other similar causes.  (+info)

Differential chemosensitivity of breast cancer cells to ganciclovir treatment following adenovirus-mediated herpes simplex virus thymidine kinase gene transfer. (4/413)

The development of resistance to radiation and chemotherapeutic agents that cause DNA damage is a major problem for the treatment of breast and other cancers. The p53 tumor suppressor gene plays a direct role in the signaling of cell cycle arrest and apoptosis in response to DNA damage, and p53 gene mutations have been correlated with increased resistance to DNA-damaging agents. Herpes simplex virus thymidine kinase (HSV-tk) gene transfer followed by ganciclovir (GCV) treatment is a novel tumor ablation strategy that has shown good success in a variety of experimental tumor models. However, GCV cytotoxicity is believed to be mediated by DNA damage-induced apoptosis, and the relationship between p53 gene status, p53-mediated apoptosis, and the sensitivity of human tumors to HSV-tk/GCV treatment has not been firmly established. To address this issue, we compared the therapeutic efficacy of adenovirus-mediated HSV-tk gene transfer and GCV treatment in two human breast cancer cell lines: MCF-7 cells, which express wild-type p53, and MDA-MB-468 cells, which express high levels of a mutant p53 (273 Arg-His). Treating MCF-7 cells with AdHSV-tk/GCV led to the predicted increase in endogenous p53 and p21WAF1/CIP1 protein levels, and apoptosis was observed in a significant proportion of the target cell population. However, treating MDA-MB-468 cells under the same conditions resulted in a much stronger apoptotic response in the absence of induction in p21WAF1/CIP1 protein levels. This latter result suggested that HSV-tk/GCV treatment can activate a strong p53-independent apoptotic response in tumor cells that lack functional p53. To confirm this observation, four additional human breast cancer cell lines expressing mutant p53 were examined. Although a significant degree of variability in GCV chemosensitivity was observed in these cell lines, all displayed a greater reduction in cell viability than MCF-7 or normal mammary cells treated under the same conditions. These results suggest that endogenous p53 status does not correlate with chemosensitivity to HSV-tk/GCV treatment. Furthermore, evidence for a p53-independent apoptotic response serves to extend the potential of this therapeutic strategy to tumors that express mutant p53 and that may have developed resistance to conventional genotoxic agents.  (+info)

Evanescent-wave microscopy: a new tool to gain insight into the control of transmitter release. (5/413)

Evanescent-wave excitation was used to visualize individual fluorescently labelled vesicles in an optical slice near the plasma membrane of bovine adrenal chromaffin cells. A standard upright microscope was modified to accommodate the optics used for directing a laser beam under a supracritical angle on to the glass-water interface on top of which the cells are grown. Whereas epi-illumination images appeared blurred and structureless, evanescent-wave excitation highlighted acridine orange-labelled vesicles as individual pinpoints. Three-dimensional (3D) trajectories of individual vesicles were obtained from time-resolved image stacks and used to characterize vesicles in terms of their average fluorescence F and mobility, expressed here as the 3D diffusion coefficient D(3). Based on the single-vesicle analysis, two groups of vesicles were identified. Transitions between these states were studied before and after stimulation of exocytosis by repetitive or maintained membrane depolarizations by elevated extracellular [K+]. Findings were interpreted as sequential transitions between the previously characterized pools of vesicles preceding the fusion step. The observed approach of vesicles to their docking sites was not explained in terms of free diffusion: most vesicles moved unidirectionally as if directed to their binding sites at the plasma membrane. Vesicle mobility at the membrane was low, such that the sites of docking and fusion were in close vicinity. Both the rim region and confined areas in the centre of the footprint region were the site of intense vesicle trafficking.  (+info)

Arrest of endosome acidification by bafilomycin A1 mimics insulin action on GLUT4 translocation in 3T3-L1 adipocytes. (6/413)

In insulin-sensitive fat and muscle cells, the major glucose transporter GLUT4 is constitutively sequestered in endosomal tubulovesicular membranes, and moves to the cell surface in response to insulin. While sequence information within GLUT4 appears to be responsible for its constitutive intracellular sequestration, the regulatory elements and mechanisms that enable this protein to achieve its unique sorting pattern under basal and insulin-stimulated conditions are poorly understood. We show here that arrest of endosome acidification in insulin-sensitive 3T3-L1 adipocytes by bafilomycin A1, a specific inhibitor of the vacuolar proton pump, results in the rapid and dose-dependent translocation of GLUT4 from the cell interior to the membrane surface; the effects of maximally stimulatory concentrations of bafilomycin A1 (400-800 nM) were equivalent to 50-65% of the effects of acute insulin treatment. Like insulin, bafilomycin A1 induced the redistribution of GLUT1 and Rab4, but not that of other proteins whose membrane localization has been shown to be insulin-insensitive. Studies to address the mechanism of this effect demonstrated that neither autophosphorylation nor internalization of the insulin receptor was altered by bafilomycin A1 treatment. Bafilomycin-induced GLUT4 translocation was not blocked by cell pretreatment with wortmannin. Taken together, these data indicate that arrest of endosome acidification mimics insulin action on GLUT4 and GLUT1 translocation by a mechanism distal to insulin receptor and phosphatidylinositol 3-kinase activation, and suggest an important role for endosomal pH in the membrane dynamics of the glucose transporters.  (+info)

Radiation-induced leukocyte entrapment in the rat retinal microcirculation. (7/413)

PURPOSE: To evaluate the effects of irradiation on leukocyte dynamics in the rat retinal microcirculation. METHODS: Thirty-five Brown-Norway rats received a dose of 10 Gy irradiation in one fraction. Leukocyte dynamics were studied with acridine orange digital fluorography, in which a nuclear fluorescent dye of acridine orange is used and examined by scanning laser ophthalmoscope. This technique allowed visualization of fluorescent leukocytes in vivo. The leukocyte dynamics were evaluated at 0, 4, 7, 14, 30, and 60 days after the irradiation. RESULTS: Mean leukocyte velocity in the retinal capillaries decreased immediately. It partially recovered on day 4 but then gradually decreased up to the 2-month mark. Low-dose irradiation induced entrapment of leukocytes in the retinal microcirculation. The number of entrapped leukocytes gradually increased with time. The major retinal vessels significantly constricted immediately after irradiation. The diameter was reduced by 76% in arteries and 75% in veins, 2 months after irradiation. CONCLUSIONS: Entrapped leukocytes may be activated and exacerbate vascular injury and microinfarction and thus may participate in the pathogenesis of radiation retinopathy.  (+info)

Tick-borne relapsing fever imported from West Africa: diagnosis by quantitative buffy coat analysis and in vitro culture of Borrelia crocidurae. (8/413)

West African tick-borne relapsing fever (TBRF) is difficult to diagnose due to the low number of spirochetes in the bloodstream of patients. Previously, the causative microorganism, Borrelia crocidurae, had never been cultured in vitro. TBRF was rapidly diagnosed for two patients returning from western Africa with fever of unknown origin by quantitative buffy coat (QBC) analysis. Diagnosis was confirmed by intraperitoneal inoculation of blood specimens from patients into laboratory mice. In vitro experiments showed that QBC analysis may be as much as 100-fold more sensitive than thick smear. Spirochetes were also cultured from blood samples from both patients in modified Kelly's medium and were identified as B. crocidurae by partial sequencing of the PCR-amplified rrs gene.  (+info)

Purified Mitochondrial Membrane Potential Assay Kit (Orange Fluorescence) from Creative Biomart. Mitochondrial Membrane Potential Assay Kit (Orange Fluorescence) can be used for research.
Purified Fixable Dead Cell Staining Kit (Orange Fluorescence 405) from Creative Biomart. Fixable Dead Cell Staining Kit (Orange Fluorescence 405) can be used for research.
Components: s2146 Acridine Orange Stock Solution s1656 Sorensens Phosphate Buffer pH 6.0 s2147 Calcium Chloride 0.1M Solution s100 Acetic Acid 1% Aqueous
For speedy, intra-operative pathological margin assessment to steer staged cancers excisions, multimodal confocal mosaic check picture wide surgical margins (approximately 1?cm) with sub-cellular resolution and mimic the appearance of conventional hematoxylin and eosin histopathology (H&E). The goal of this work is definitely to combine three confocal imaging modes: acridine orange fluorescence (AO) for labeling nuclei, […]. ...
We do routinely use acridine orange using FACScan. A 20 min rinse with 10 times water-diluted Chlorox bleach is adequate to clean the instrument. Zbigniew Darzynkiewicz -----Original Message----- From: Karen Creswell [mailto:creswelk at georgetown.edu] Sent: Friday, June 04, 2004 1:17 PM To: cyto-inbox Subject: Acridine Orange Does anyone routinely run acridine orange through their FACScan/FACSort? I would like suggestions for cleaning the instrument afterwards. Thanks for your help, Karen -- Karen Creswell, Ph.D. Director, Flow Cytometry/Cell Sorting Shared Resource Lombardi Cancer Center Georgetown University Medical Center Research Bldg W304 Box 571469 Washington, DC 20057-1469 202-687-2233 creswelk at georgetown.edu ...
... ,Application: Acridine orange has been used as a fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Acridine orange at a concentration of 120 μM will detect purified DNA in gels with a sensitivity of 25-50 ng per band. It has also been used extensively for cell staining of,biological,biology supply,biology supplies,biology product
The adsorption of the metachromic dye acridine orange (AO) by Na-beidellite was investigated by visible spectroscopy. Different types of clay-AO association were identified from the appearance and location of absorption bands alpha or beta . The colloidal properties were determined from curves of the absorbance vs. the degree of saturation. Three regions were identified in the absorbance curve. In the first region beidellite is peptized with small amounts of AO and the dye penetrates into the interlayer space where it undergoes metachromasy due to pi interactions between the aromatic entity and the oxygen plane of the clay. With larger amounts of AO (second region), the clay flocculates due to the aggregation of the dye cations in the interparticle space of the flocs. In excess AO (third region), beidellite is gradually peptized, forming small tactoids with monomeric AO in the interlayer space and at the same time adsorbing dimeric and polymeric AO cationic species at the solid-liquid interface. ...
Sixteen marine isolates from a NORPAX cruise, which were transferred once on medium after initial isolation, survived nutrient deprivation for at least 8 months (longest period test). All but one isolate remained cellularly intact, although their sizes and shapes changed greatly, and all became smaller, decreasing in size from 40 to 79%. Three starvation-survival patterns were demonstrated, namely (i) an initial increase in viable cells followed by a decrease until a constant number was reached, (ii) an increase in viable cells until a constant number was reached, and (iii) a decrease in viable cells until a constant number was reached. One isolate from each starvation-survival pattern was starved for 8 months and then was tested in comparison with 4-month-starved Ant-300 for [14C]glutamic acid uptake, respiration, and incorporation. The response to glutamic acid was rapid and linear in each case. The data indicate that the starvation-survival of Ant-300 is not an anomalous situation and that ...
BioAssay record AID 693243 submitted by ChEMBL: Induction of apoptosis in human MGC803 cells assessed as pyknosis at 5 uM after 12 to 48 hrs by acridine orange/ethidium bromide staining.
Acridine Orange: A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.
DNA intercalating dye. A grade of acridine orange of exceptionally high purity, suitable for quantitative work. Free of inorganic salts.
HeLa cells. Light micrograph (acridine orange stain) of a culture of HeLa cancer cells. The nuclei of the cells are stained yellow while the cytoplasm is orange. HeLa cells are the first human cell line established in 1952 to research cancer. They were obtained from the cervix of Henrietta Lacks (after whom the cells are named) from Baltimore, USA, in 1951, who died of cervical cancer eight months later. HeLa cells thrive unusually well in the laboratory and are used in research worldwide. Professor Anderson (1911-2006) was the first to use acridine orange staining to study cells, bacteria and viruses. - Stock Image G442/0059
Background: Mitochondrial permeabilization provokes cardiomyocyte death, and causes adverse left ventricular remodeling in cardiac-restricted TNF transgenic mice (MHCsTNF). Autophagy, a lysosomal degradative pathway, is essential for removal of ubiquitin-tagged damaged mitochondria. Our prior work suggests that TNF receptor associated factor-2 (TRAF2), a multifunctional protein with E3 ubiquitin ligase activity, facilitates removal of damaged mitochondria. The role of TRAF2 vis-à-vis PARKIN, another E3 ubiquitin ligase essential for mitophagy, is unknown.. Methods and Results: MHCsTNF hearts and TNF-treated neonatal rat cardiac myocytes (NRCMs) demonstrate upregulation of both TRAF2 and PARKIN, with increased evidence for mitochondrial autophagy and reduced mitochondrial mass. To evaluate the role of TRAF2 and PARKIN in autophagic removal of damaged mitochondria, we employed gain-of-function and loss-of-function approaches; and assessed mitochondrial mass with nonyl-acridine orange fluorescence ...
Stain F-actin in cell samples with F-actin Staining Kit - Orange Fluorescence - Cytopainter ab112126. For fluorescence microscopes.
A while ago, scientists at the National University of Singapore created transgenic, fluorescent zebra fish by injecting red, green, and orange fluorescence genes from a jellyfish into the fish eggs. They did this so the fish could help detect environmental pollutants.Now, you can actually have them for pets! They are marketed under the name GloFish, and look very cool under a black light. Link - Thanks moniA!Previously on Neatorama: Fluorescent pig, rabbit, butterfly, and frog...
The metal chelating compound Dp44mT is a di-2-pyridylketone thiosemicarbazone (DpT) which displays potent and selective anti-tumor activity. This compound is receiving translational attention but its mechanism is poorly understood. Here we report that Dp44mT targets lysosome integrity through copper binding. Studies using the lysosomotropic fluorochrome acridine orange established that the copper-Dp44mT complex (Cu[Dp44mT]) disrupted lysosomes. This targeting was confirmed with pepstatin A-BODIPY FL, which showed re-distribution of cathepsin D to the cytosol with ensuing cleavage of the pro-apoptotic BH3 protein Bid. Redox activity of Cu[Dp44mT] caused cellular depletion of glutathione and lysosomal damage was prevented by co-treatment with the glutathione precursor N-acetylcysteine. Copper binding was essential for the potent anti-tumor activity of Dp44mT, since co-incubation with non-toxic copper chelators markedly attenuated its cytotoxicity. Taken together, our studies show how the lysosomal ...
The majority of phosphatase (PO4ase) activity detected in fresh aerobic activated sludge from a municipal wastewater treatment plant was associated with suspended floc material. PO4ase activity appeared to be localized in discrete bacteria-containing areas of the floc matrix based on the distribution of nucleic acid-stained cells and precipitated fluorescent crystals produced as a result of reaction of the enzyme(s) with the artificial substrate ELF™-PO4. Of the total floc-associated bacterial cells that stained positive with the nucleic acid-binding fluorochrome acridine orange (AO), 8.8 ± 1.2% displayed PO4ase activity based on the proximity of AO-stained cells to precipitated ELF crystals. Using a 16S rRNA oligonucleotide probe specific for the cytophaga-flavobacteria group, it was determined that 17-20% of the floc-associated bacteria that probed positive also displayed PO4ase activity. Furthermore, 35-45% of the ELF fluorescence was associated with bacterial cells that probed positive ...
Name:C.I.Basic Orange 14,C.I.46005 Molecular Structure: Acridines class C.I.Basic Orange 14,C.I.46005,CAS 65-61-2,301.81,C17H20ClN3,Acridine Orange C.I.Basic Orange 14,C.I.46005,CAS 65-61-2,301.81,C17H20ClN3,Acridine Orange Molecular Formula:C17H20ClN3 Molecular Weight: 301.81 CAS Registry Number:65-61-2
Aims: In view of recent findings that a multidrug efflux pump CmeABC exists in Campylobacter jejuni, 391 C. jejuni and 52 Campylobacter coli of human and animal origin were examined for a multidrug resistance phenotype.. Materials and methods: The MICs of ampicillin, chloramphenicol, ciprofloxacin, erythromycin, kanamycin, tetracycline, cetrimide, triclosan, acridine orange, paraquat and ethidium bromide were determined. Resistance to organic solvents and the effect of salicylate (known inducer of the marRAB operon in Escherichia coli and Salmonella) were also examined.. Results: Two C. coli and 13 C. jejuni isolates, mainly from pigs or poultry, were resistant to three or more antibiotics and 12 of these strains had reduced susceptibility to acridine orange and/or ethidium bromide. Strains (n = 20) that were less susceptible to acridine orange, ethidium bromide and triclosan were significantly more resistant (P , 0.05) to ampicillin, chloramphenicol, ciprofloxacin, erythromycin, nalidixic acid ...
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Acridine orange is a nucleic acid staining dye that is used to identify dead cells. It intercalates with the double-stranded DNA base pairs at a ratio of 1:3 and is capable of emitting green fluorescence (Ex: 500 nm, Em: 520 nm). It also emits red fluorescence (Ex: 460 nm, Em: 650 nm) when bound to RNA or single-stranded DNA. Since acridine orange is mutagenic, this product is supplied as a solution that prevents it from splashing during weighing.. ...
During human spermiogenesis, the elongated spermatids undergo a plasma membrane remodeling step that facilitates formation of the zona pellucida and hyaluronic acid (HA) binding sites. Various biochemical sperm markers indicated that human sperm bound to HA exhibit attributes similar to that of zona …
The accuracy of techniques for the diagnosis of malaria are usually compared with optical microscopy, which is considered to be a gold standard. However, microscopy is prone to error and therefore makes it difficult to assess the reliability of other diagnostic techniques. We did a systematic review to assess the specificity and sensitivity of diagnostic techniques in different settings, using a statistical method that avoided defining a gold standard. Performance varied depending on species of the malaria parasite, level of parasitaemia, and immunity. Overall, histidine-rich protein 2 (HRP2)-based dipsticks showed a high sensitivity (92.7%) and specificity (99.2%) for Plasmodium falciparum in endemic areas. The acridine orange test was more sensitive (97.1%) in detecting P falciparum in epidemiological studies, with a specificity of 97.9%. In the absence of a gold standard, HRP2 dipsticks and acridine orange could provide an alternative for detecting falciparum infections in endemic areas and
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PromoKine offers a wide range of dyes for fluorescently staining dead cells. The membrane-impermeable nuclear stains can also be used for fixed cells. Commonly used dyes for staining dead cells include; orange-fluorescent ethidium bromide (EB), red-fluorescent propidium iodide (PI), green-fluorescent acridine orange (AO) and blue-fluorescent DAPI. The toluidine derivative Trypan Blue is a classical non-fluorescent dye used to selectively stain dead cells and shows a distinctive blue colour under the microscope. ...
A quiescent [denoted as Q(G0/G1)] subpopulation was isolated from EMT6/Ro-fed plateau monolayers by centrifugal elutriation. The median Coulter volume of these cells was significantly smaller than that of the original population from which they were elutriated. Using two-step acridine orange staining and dual parameter flow cytometric analysis, over 95% of quiescent cells were found to have G1 DNA content, and 80% of the cells had a decreased RNA content as compared to rapidly proliferating exponential G1 cells. After labeling for 24 hr (two doubling times) with [3H]thymidine, less than 2% of the quiescent cells incorporated [3H]thymidine as measured by autoradiography. The colony-forming efficiency of these cells was not significantly different from that of exponential cells. When such Q(G0/G1) cells were replated in fresh medium at a lower density, there was a lag time of 30 hr before any increase in cell number was detected, after which the cell-doubling rate matched that of exponential ...
contact Luis Benitez at Oncological Research Unit, kAP 107-014, UNAM, Hosp. 21 Sieglo, Mexico DF 06700, Mexico. He has some interesting results along this line. In article ,905kf544 at invrof.anmat.sld.ar, elisa at invrof.anmat.sld.ar (Dra. Elisa Bal de Kier-Joffe) writes: ,Dear netters: ,We are using Acridine Orange (AO) to stain apoptotic cells. , ,The nuclei staining of apoptotic cells is very easily recognized when it is ,in an advanced stage. ,However when the process is early I observe only a brighter staining of ,chromatin clumps, and a more marked limit in nuclei periphery. When this ,happens, the cytoplasm shows a diffuse red staining which is also evident in ,definite apoptotic cells. , ,In contrast normal alive cells only show a pale diffuse green staining of ,the nuclei and cytoplasm and only a kind of red granule (RNA) in the ,cytoplasm next to the nucleus. , ,I have not found any comments concerning this early diffuse intense red ,staining of the cytoplasm with AO in apoptotic ...
A novel method for the detection, screening, the quantitation of compounds that interact with nucleic acids (DNA or RNA) is provided. The basis for this method is the inhibition of acridine orange binding to the nucleic acid. Measurement of binding inhibition utilizes the rapid, repeatable technique of fluorescence polarization.
Fluorescent micrographs and quantitative analysis of CAOV-3 cells double-stained with acridine orange and propidium iodide. Cells were treated with liriodenine
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17784-47-3 - XUESTGHCVFYOLL-UHFFFAOYSA-N - Acridine, hydrochloride - Similar structures search, synonyms, formulas, resource links, and other chemical information.
Fruit can be divided into a good many varieties; especially oranges are popular fruit. Oranges are eaten by many ways; such as the orange drink. The process of manufacturing orange drink can be divided into four stages: picking and preparation of the f...
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Deep, Dark Salted Butter Caramel Sauce, for serving (after using Zoe Nathans, decided that I definitely prefer my own, reprinted below). 1. In a food processor, pulse the 1 cup of flour with 2 tablespoons of the sugar and the baking powder and salt. Add the stick of cold butter and pulse several times, just until it is the size of peas. Sprinkle the dough with the ice water and pulse just until moistened crumbs form. Turn the crumbs out onto a work surface, knead once or twice and pat the pastry into a disk. Wrap the pastry in plastic and chill for 30 minutes.. 2. On a floured work surface, roll out the pastry to an 11-inch round, about 1/4 inch thick. Transfer the pastry to a parchment paper-lined flat cookie sheet and refrigerate for 15 minutes, or until chilled.. 3. Meanwhile, peel the blood oranges, removing all of the bitter white pith. Thinly slice 2 of the oranges crosswise; remove the pits. Transfer the orange slices to a plate. Working over a sieve set over a bowl, cut in between the ...
Deep, Dark Salted Butter Caramel Sauce, for serving (after using Zoe Nathans, decided that I definitely prefer my own, reprinted below). 1. In a food processor, pulse the 1 cup of flour with 2 tablespoons of the sugar and the baking powder and salt. Add the stick of cold butter and pulse several times, just until it is the size of peas. Sprinkle the dough with the ice water and pulse just until moistened crumbs form. Turn the crumbs out onto a work surface, knead once or twice and pat the pastry into a disk. Wrap the pastry in plastic and chill for 30 minutes.. 2. On a floured work surface, roll out the pastry to an 11-inch round, about 1/4 inch thick. Transfer the pastry to a parchment paper-lined flat cookie sheet and refrigerate for 15 minutes, or until chilled.. 3. Meanwhile, peel the blood oranges, removing all of the bitter white pith. Thinly slice 2 of the oranges crosswise; remove the pits. Transfer the orange slices to a plate. Working over a sieve set over a bowl, cut in between the ...
Pill with imprint SHIRE 381 30mg is Orange / Orange, Capsule-shape and has been identified as Adderall XR 30 mg. It is supplied by Shire US Inc..
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Youre in the right place if you want to avoid the orange, and who doesnt. Lets begin by explaining how skin can turn orange. Over-application (that can be too much in one application and/or applying too frequently), using budget brands that use lower-quality ingredients, and applying when your skin is noticeably dry. Follow these tips to lessen the dreaded orange:. ...
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What does God want for your life? What is Gods plan for you? Want some orange drink? These were the questions people asked me on Sundays when I was growing up as a teenager in church. We wondered about what God wanted for us, and wondered how we all could hold ourselves and our friends accountable for our behavior to keep us walking in the path of holiness. All, of course, discussed over the ubiquitous orange drink served at youth meetings across the country. Orange drink is the most righteous of bulk beverages.
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Protein content and RDA percentage, per serving and per 100g, in 26 types of an orange. The amount of Protein is 3.6 g to 0 g per 100g, in an orange.
Acridine orange[edit]. Acridine orange (AO) is a nucleic acid selective fluorescent cationic dye useful for cell cycle ... The stain may also be used in conjunction with acridine orange (AO) in viable cell counting. This EB/AO combined stain causes ... It uses a combination of haematoxylin, Orange G, eosin Y, Light Green SF yellowish, and sometimes Bismarck Brown Y. ... In a skilfully made H & E preparation the red blood cells are almost orange, and collagen and cytoplasm (especially muscle) ...
Acridine derivatives: proflavin, acridine orange, acridine yellow, etc. Arylmethine derivatives: auramine, crystal violet, ... DRAQ7 and CyTRAK Orange Pyrene derivatives: cascade blue, etc. Oxazine derivatives: Nile red, Nile blue, cresyl violet, oxazine ...
Acta 883: 173-177 Notes Darzynkiewicz Z, Kapuscinski J. (1990)"Acridine Orange, a Versatile Probe of Nucleic Acids and Other ... Another example of metachromatic dye (fluorochrome) is acridine orange. Under certain conditions it stains single-stranded ...
Hathaway WE, Newby LA, Githens JH (1964). "THE ACRIDINE ORANGE VIABILITY TEST APPLIED TO BONE MARROW CELLS. I. CORRELATION WITH ...
Darzynkiewicz Z, Kapuscinski J. (1990)"Acridine Orange, a Versatile Probe of Nucleic Acids and Other Cell Constituents." ... Another example of metachromatic dye (fluorochrome) is acridine orange. Under certain conditions it stains single-stranded ...
Cell apoptosis is tested by treating the lysosomal membrane with acridine orange. Acridine orange radiates a red fluorescent ...
Traganos F, Darzynkiewicz Z.(1994) Lysosomal proton pump activity: supravital cell staining with acridine orange differentiates ...
1977). Bacterial cells were counted with an epifluorescence microscope, producing what is called an "acridine orange direct ...
Several dyes have been studied for MUSE's application, including eosin, rhodamine, DAPI, Hoechst, acridine orange, propidium ...
The blood is taken in a QBC capillary tube which is coated with acridine orange (a fluorescent dye) and centrifuged; the ...
Affected cells do not exhibit hallmarks of apoptosis but rather autophagy as seen by staining with acridine orange and ...
B. turicatae is best visualized by dark-field microscopy, but the organisms can also be detected using acridine orange or ...
M Garciafernandez; D Ceccarelli; U Muscatello (2004). "Use of the fluorescent dye 10-N-nonyl acridine orange in quantitative ... Jacobson J, Duchen MR, Heales SJ (2002). "Intracellular distribution of the fluorescent dye nonyl acridine orange responds to ... Based on this special structure, the fluorescent mitochondrial indicator, nonyl acridine orange (NAO) was introduced in 1982, ... "Staining of mitochondrial membranes with 10-nonyl acridine orange, MitoFluor Green, and Mito-Tracker Green is affected by ...
Apoptosis was evaluated by flow cytometry on PBMC from HIV infected individuals (cells were stained with acridine orange and ...
... as revealed by acridine orange staining and PCR-based diagnoses". Tropical Medicine & International Health. 3 (4): 304-312. doi ...
... acridine orange stain, and phase-contrast microscopy. It is capable of forming biofilms and can convert from spiral to a ...
An exception is the metachromatic fluorochrome acridine orange, which under the specific staining protocol can differentially ... or DNA susceptibility to denaturation at low pH using the metachromatic dye acridine orange, reveals the G1Q, G1A, and G1B cell ... Aside from propidium iodide and acridine orange, quantifiable dyes that are frequently used include (but are not limited to) ...
... acridine orange MeSH D03.494.046.250.177 --- acriflavine MeSH D03.494.046.250.200 --- aminacrine MeSH D03.494.046.250.225 --- ...
Further details can be found in the IARC Monographs Acenaphthene Acepyrene Aciclovir Acridine orange Acriflavinium chloride ... acridine Benz[c]acridine Benzo[g]chrysene Benzo[a]fluoranthene Benzo[ghi]fluoranthene Benzo[a]fluorene Benzo[b]fluorene Benzo[c ... Orange I Orange G Oxyphenbutazone Palygorskite (attapulgite) (short fibres, < 5 micrometers) Paracetamol (Acetaminophen) ... metallic Chrysene Chrysoidine CI Acid Orange 3 Cimetidine Cinnamyl anthranilate CI Pigment Red 3 Citrinin Clofibrate Clomiphene ...
Psoralens, coal tars, photo-active dyes (eosin, acridine orange) Musk ambrette, methylcoumarin, lemon oil (may be present in ... In these areas an unsightly orange to brown tint may form, usually near or on the face. Many medications and conditions can ...
The flow-cytometric assay utilizing the fluorochrome acridine orange shows that DNA fragmentation within individual cells is ...
Ethidium bromide GelRed SYBR Green I Agarose gel electrophoresis and gel electrophoresis of nucleic acids Acridine orange US ... GelGreen is structurally closely related to acridinium orange and consists of two acridinium orange subunits that are bridged ... Its fluorophore, and therefore its optical properties, are essentially identical to those of other N-alkylacridinium orange ...
... and then in 1985 of the F plasmid by acridine orange curing.[citation needed] Strains derived from MG1655 include DH1, parent ...
... and related derivatives (such as amsacrine) bind to DNA and RNA due to their abilities to intercalate. Acridine orange ... Acridine is easily oxidized by peroxymonosulfuric acid to the acridine amine oxide. The carbon 9-position of acridine is ... biologically active acridines, applications of acridines, new syntheses and reactions of acridines] Synthesis of acridone in ... Acridine and its homologues are weakly basic. Acridine is a photobase which have a ground state pKa of 5.1, which is similar to ...
... damages DNA and is used as a mutagen in microbiology. Acridine yellow is similar to acridine orange. Acridine ... Acridine yellow, also known as acridine yellow G, acridine yellow H107, basic yellow K, and 3,6-diamino-2,7-dimethylacridine, ... It is a derivate of acridine. In histology, it is used as a fluorescent stain, and as a fluorescent probe for non-invasive ...
... orange).[160] ... Acridine derivatives[104] Anthranilic acid [113] Rutacridone, ...
... using acridine orange is specified in methods for the microbial examination of food and water. The use of acridine orange in ... and seawater samples can be achieved using acridine orange. Acridine orange direct count (AODC) methodology has been used in ... Acridine orange emission changes from yellow, to orange, to red fluorescence as the pH drops in acidic vacuole of the living ... Acridine orange is useful for enumerating the microbes in a sample. Acridine dyes are prepared via the condensation of 1,3- ...
Acridine orange has been used as a fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Acridine orange at a ... Acridine Orange hemi(zinc chloride) salt from Sigma-Aldrich,Application: ... Acridine orange; 10 mg/mL solution in water from AnaSpec. 2. Acridine orange 10-nonyl bromide from AnaSpec. 3. Acridine orange ... Acridine orange has been used as a fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Acridine orange at a ...
... acridine hydrochloride zinc chloride double salt (CAS 10127-02-3), a cell-permeable metachromatic fluorescent cationic DNA and ... Acridine orange for cell sorting of sputum.. J Histochem Cytochem 27:552-6 (1979). Read more (PubMed: 86576) » ... The use of thionin and acridine orange in staining semithin sections of plant material embedded in epoxy resin.. Stain Technol ... Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. ...
The use of acridine orange stained buffy coat smears was assessed as a rapid screening test for bacteremia in adults. A total ... Rapid Diagnosis of Bacteremia in Adults Using Acridine Orange Stained Buffy Coat Smears. Mark Miller1,2 and Jack Mendelson1 ... Compared to blood culture, the overall sensitivity of acridine orange stained buffy coat smears was 16%, specificity 88%, and ... from which a buffy coat smear was prepared and stained with acridine orange (100 mg/L; pH 3.0). Forty-one of 356 blood samples ...
Acridine Orange: A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and ... Acridine Orange; Diamine, Tetramethyl Acridine; Orange 3RN, Basic; Orange Base, Acridine; Orange, Acridine; Orange, Rhoduline; ... 3,6-Bis(dimethylamino)acridine; Acridine Orange Base; Basic Orange 3RN; C.I. 46005; C.I. Basic Orange 14; Rhoduline Orange; ... Acridine Orange. Subscribe to New Research on Acridine Orange A cationic cytochemical stain specific for cell nuclei, ...
... Joanne Lannigan joannelannigan at virginia.edu Mon Jun 7 15:20:40 EST 2004 *Previous message: Acridine Orange ... Acridine Orange , , Does anyone routinely run acridine orange through their , FACScan/FACSort? I would like suggestions for ...
... Does anyone routinely run acridine orange through their FACScan/FACSort? I would like suggestions for cleaning ... Acridine Orange. DARZYNKIEWICZ ZBIGNIEW Z_DARZYNKIEWICZ at nymc.edu Mon Jun 7 14:47:50 EST 2004 *Previous message: Parts to ... We do routinely use acridine orange using FACScan. A 20 min rinse with 10 times water-diluted Chlorox bleach is adequate to ...
Diagnosis of malaria by acridine orange fluorescent microscopy in an endemic area of venezuela.. Bosch I1, Bracho C, Pérez HA. ... Fluorescent (acridine orange) microscopical examination of capillary centrifuged blood (quantitative buffy coat [QBC] analysis ...
A grade of acridine orange of exceptionally high purity, suitable for quantitative work. Free of inorganic salts. ... Polysciences grade of acridine orange is of exceptionally high purity, suitable for quantitative work. It is free of inorganic ... Acridine orange is a nucleic acid-selective fluorescent cationic dye useful for cell cycle determination. It emits green ... We offer a highly purified form of acridine orange while most of the other commercially available grades are either of low ...
By Leo Chan,2018-06-20T20:27:59+00:00May 20th, 2015,Categories: Cellometer Application News,Tags: Acridine Orange, Propidium ...
Spermatozoa bound to solid state hyaluronic acid show chromatin structure with high DNA chain integrity: an acridine orange ... we tested the hypothesis that HA-bound sperm would be enhanced in sperm of high DNA chain integrity and green acridine orange ...
Comparison of the automicrobic system, acridine orange-stained smears, and gram-stained smears in detecting bacteriuria.. B A ... We compared the accuracy of the Gram-stained smear, the acridine orange-stained smear, and the AutoMicrobic system (AMS; Vitek ... For 1,024 clinical specimens, results with the acridine orange-stained smear and the Gram-stained smear were very similar. When ... Comparison of the automicrobic system, acridine orange-stained smears, and gram-stained smears in detecting bacteriuria. ...
Acridine orange staining was used to visualize apoptosis in A172 (A) and U87 (B). Cells were treated with 0, 10 or 100 n ... Acridine orange staining was used to visualize apoptosis in A172 (A) and U87 (B). Cells were treated with 0, 10 or 100 ng/ml ... Acridine orange staining was used to visualize apoptosis in A172 (A) and U87 (B). Cells were treated with 0, 10 or 100 ng/ml ... After 24h, cells were stained using acridine orange dye. Mentions: To confirm that the combinatorial effects described above ...
Acridine Orange, 10 mg/mL in Water (High Purity) (AO) Acridine orange (AO) stains dsDNA green (525 nm) and RNA or single ... Acridine orange (AO) stains dsDNA green (525 nm) and RNA or single stranded DNA red (650 nm). The dye is membrane-permeant and ... 3 Acridine orange has also been used for the detection of microorganisms in cerebrospinal fluid and other clinical specimens.We ... offer a highly purified form of acridine orange while most of the other commercially available grades of AO are either in zinc ...
Zhang, Y.; Pan, J.; Zhang, G.; Zhou, X. Intercalation of herbicide propyzamide into DNA using acridine orange as a fluorescence ... Silica nanoparticles assisted photodegradation of acridine orange in aqueous suspensions. Appl. Catal., B 2015, 168-169, 363- ... An RNA polymerase inhibitorAcridine Orange acts as a lysosomal dye. It is used for cell-cycle studies. It plays an important ...
Looking for abbreviations of AO/PI? It is Acridine Orange and Propidium Iodide. Acridine Orange and Propidium Iodide listed as ... Acridine Orange and Propidium Iodide - How is Acridine Orange and Propidium Iodide abbreviated? https://acronyms. ... a href=https://acronyms.thefreedictionary.com/Acridine+Orange+and+Propidium+Iodide,AO/PI,/a,. *Facebook ... redirected from Acridine Orange and Propidium Iodide) Acronym. Definition. AO/PI. Acridine Orange and Propidium Iodide. ...
The study revealed that the Acridine technique was at least as sensitive as the older Giemsa technique, but proved quicker and ... The differentiation of Wuchereria banerofti and Dipetalonema perstans microfilariae when stained with Acridine is discussed. ... The present study compares the value of the Acridine Orange technique and the Giemsa staining technique for the detection of ... oa Central African Journal of Medicine - The use of acridine orange 0 in the laboratory diagnosis of human filariasis * ...
Zelenka, K; Borsig, L; Alberto, R (2011). Metal complex mediated conjugation of peptides to nucleus targeting acridine orange: ... With this concept, we have conjugated the acridine orange part to a bombesin peptide directly on the (99m)Tc core and in one ... With this concept, we have conjugated the acridine orange part to a bombesin peptide directly on the (99m)Tc core and in one ... We have synthesized acridine orange derivatives which comprise an imidazole-2-carbaldehyde function for coordination to the Re( ...
"Utility of Acridine Orange staining for detection of bacteria from positive blood cultures, Journal Of Microbiological Methods ... Utility of Acridine Orange staining for detection of bacteria from positive blood cultures. Utility of Acridine Orange staining ... Utility of Acridine Orange staining for detection of bacteria from positive blood cultures. Neeraja, M.; Lakshmi, V.; Padmasri ... Evaluation of acridine orange staining as a replacement of subcultures for BacT/ALERT-positive, Gram stain-negative blood ...
... acridine explanation free. What is acridine? Meaning of acridine medical term. What does acridine mean? ... Looking for online definition of acridine in the Medical Dictionary? ... Acridine orange stains nucleic acid so that under ultraviolet light DNA appears green and RNA appears orange.. acridine (orange ... acridine. Also found in: Dictionary, Encyclopedia, Wikipedia.. Related to acridine: acridine dyes anthracene. [an´thrah-sēn] a ...
Metachromasy in clay dye systems; the adsorption of acridine orange by Na-beidellite D. Garfinkel-Shweky D. Garfinkel-Shweky ... D. Garfinkel-Shweky, S. Yariv; Metachromasy in clay dye systems; the adsorption of acridine orange by Na-beidellite. Clay ... The adsorption of the metachromic dye acridine orange (AO) by Na-beidellite was investigated by visible spectroscopy. Different ... Metachromasy in clay-dye systems; the adsorption of acridine orange by Na-saponite Clay Minerals ...
Acridine orange[edit]. Acridine orange (AO) is a nucleic acid selective fluorescent cationic dye useful for cell cycle ... The stain may also be used in conjunction with acridine orange (AO) in viable cell counting. This EB/AO combined stain causes ... It uses a combination of haematoxylin, Orange G, eosin Y, Light Green SF yellowish, and sometimes Bismarck Brown Y. ... In a skilfully made H & E preparation the red blood cells are almost orange, and collagen and cytoplasm (especially muscle) ...
Vital staining with the dye, acridine orange, has been previously used to analyze patterns of apoptosis in both vertebrate and ... Visualization of Programmed Cell Death in Whole Embryos Using Acridine Orange. Thu, 12 Jul 2012 , Situ Hybridization ... cells fragment into a number of apoptotic bodies that are chromatin-rich and are intensely stained by acridine orange. In ...
Will try new PBS first, then a concentration gradient of acridine orange. ... Acridine orange. Fri, 08/03/2012 - 20:01 - kdorfman Sigma A8097-10 mL ...
Acridine Orange, Acridine Orange for Staining which are used for various applications including medical uses, pharmaceuticals ... exporter of all laboratory chemicals including Acridine Orange for Microscopical Staining, ... Technical Specifications of Acridine Orange for Microscopical Staining :-. Zinc chloride double salt for electrophoresis ... This is done during the excitation of Acridine Orange under blue light at pH level of 3.5 while detecting the cells with the ...
  • Cell viability was measured by MTS assay and apoptosis was assessed by Annexin V/PI and acridine orange staining. (nih.gov)
  • Acridine orange staining was used to visualize apoptosis in A172 (A) and U87 (B). Cells were treated with 0, 10 or 100 ng/ml rhTRAIL WT and 0, 25 or 50 μM DMC. (nih.gov)
  • Vital staining with the dye, acridine orange, has been previously used to analyze patterns of apoptosis in both vertebrate and invertebrate embryos (3,4). (alpfmedical.info)
  • During apoptosis, cells fragment into a number of apoptotic bodies that are chromatin-rich and are intensely stained by acridine orange. (alpfmedical.info)
  • Acridine orange stain is particularly useful in the rapid screening of normally sterile specimens, and its recommended for the use of fluorescent microscopic detection of microorganisms in direct smears prepared from clinical and non-clinical materials. (wikipedia.org)
  • The use of acridine orange stained buffy coat smears was assessed as a rapid screening test for bacteremia in adults. (hindawi.com)
  • Direct staining of blood smears with acridine orange and enumeration using fluorescent microscopy allowed quantitation of Haemophilus influenzae in blood at densities from 10(5) to 10(8) cfu/ml. (curehunter.com)
  • Fluorescent (acridine orange) microscopical examination of capillary centrifuged blood (quantitative buffy coat [QBC] analysis) and Giemsa stained thick blood smears (GTS) were compared for diagnosis of malaria in blood specimens from adults living in malaria transmission areas of the States of Bolivar and Amazonas in southeastern and south Venezuela, respectively. (nih.gov)
  • Comparison of the automicrobic system, acridine orange-stained smears, and gram-stained smears in detecting bacteriuria. (asm.org)
  • Giemsa staining, acridine orange (AO) staining and semi-nested multiplex PCR. (curehunter.com)
  • The present study compares the value of the Acridine Orange technique and the Giemsa staining technique for the detection of Dipetalonema perstans in blood films. (journals.co.za)
  • The study revealed that the Acridine technique was at least as sensitive as the older Giemsa technique, but proved quicker and less tiring to use. (journals.co.za)
  • Autophagy were assessed by acridine orange staining, MDC staining and Transmission electron microscopy. (nih.gov)
  • Autophagic cell death induced by SAHA was confirmed by acridine orange staining and transmission electron microscopy (TEM) in TAMR/MCF-7 cells. (medsci.org)
  • We offer a highly purified form of acridine orange while most of the other commercially available grades are either of low purity or in zinc chloride complex form. (polysciences.com)
  • Thus, acridine orange can be used to visualize primary lysosomes and phagolysosomes that may include products of phagocytosis of apoptotic cells. (wikipedia.org)
  • At a low pH (3.5), when acridine orange is excited by blue light, it can differentially stain human cells green while staining prokaryotes bright orange for detection with a fluorescence microscope. (wikipedia.org)
  • Acridine orange is a versatile fluorescence dye used to stain acidic vacuoles (lysosomes, endosomes, and autophagosomes), RNA, and DNA in living cells. (wikipedia.org)
  • After 24h, cells were stained using acridine orange dye. (nih.gov)
  • Moreover, it helps in staining human cells with and prokaryotes with green and bright orange color, respectively. (alphachemikaindia.com)
  • This is done during the excitation of Acridine Orange under blue light at pH level of 3.5 while detecting the cells with the help of fluorescence microscope. (alphachemikaindia.com)
  • The acridine orange fluorescence stain reliably demonstrated malignant cells based on the differential fluorescence - a cytochemical criterion. (bvsalud.org)
  • However, the acridine orange fluorescence stain did not differentiate effectively between malignant cells and rapidly proliferating cells, as the technique is based on the nucleic acid content. (bvsalud.org)
  • Liapunova E.A., Zelenin A.V., The effect of acridine orange on the early steps of protein biosynthesis in tissue culture cells, Voprosy meditsinskoi khimii, 1966, vol: 12(2), 218-220. (msk.ru)
  • Cells were then stained with acridine orange 1: Control group. (nih.gov)
  • We used the acridine orange test (AOT) and the terminal deoxynucleotidyl transferase -mediated dUDP nick-end labeling ( TUNEL ) assay to assess the DNA status of sperm cells preserved with different lyophilization media. (bvsalud.org)
  • VectaCell Acridine Orange and VectCell Rhodamine 123 reagents offer convenience and ease of use for visualizing different cellular components in live cells. (vectorlabs.com)
  • The two micrographs (right) show live mononuclear cells stained with acridine orange in green, and dead cells stained with PI in red. (nexcelom.com)
  • Acridine orange has been widely accepted and used in many different areas, such as epifluorescence microscopy, the assessment of sperm chromatin quality. (wikipedia.org)
  • We do routinely use acridine orange using FACScan. (purdue.edu)
  • creswelk at georgetown.edu ] Sent: Friday, June 04, 2004 1:17 PM To: cyto-inbox Subject: Acridine Orange Does anyone routinely run acridine orange through their FACScan/FACSort? (purdue.edu)
  • We have synthesized acridine orange derivatives which comprise an imidazole-2-carbaldehyde function for coordination to the Re(CO)(3)](+) or (99)mTc(CO)(3)](+) core. (uzh.ch)
  • Derivatives of acridine are successful as antibacterial agents, finding their principal use as local antiseptics. (thefreedictionary.com)
  • Non-toxic levels of neutral red and acridine orange were suitable tracers: they were visible in the stylet and gut after feeding for 24 h, and may also attract aphids to feed. (mdpi.com)
  • Bright orange organisms are easily detected against a black to faint green background. (wikipedia.org)
  • red indicates high interlinkedness of a term with other terms, orange, yellow and green decreasing interlinkedness. (uzh.ch)
  • Acridine yellow, also known as acridine yellow G, acridine yellow H107, basic yellow K, and 3,6-diamino-2,7-dimethylacridine, is a yellow dye with strong bluish-green fluorescence. (wikipedia.org)
  • polychromatic erythrocytes (PCE) stained red/orange, nonchromatic erythrocytes (NCE) are unstained (dull green), and micronuclei stain bright yellow. (europa.eu)
  • Later on, Lauer, Reller and Mirret performed a similar study, compared acridine orange with the Gram stain for detecting the microorganisms in cerebrospinal fluid and other clinical materials. (wikipedia.org)
  • The sensitivity of Gram stain (GS) was 98.26% while Acridine Orange (AO) stain proved to be more sensitive (100%) with a Positive and Negative Predictive Value of 100% each. (deepdyve.com)