Acremonium: A mitosporic fungal genus with many reported ascomycetous teleomorphs. Cephalosporin antibiotics are derived from this genus.Plant Poisoning: Poisoning by the ingestion of plants or its leaves, berries, roots or stalks. The manifestations in both humans and animals vary in severity from mild to life threatening. In animals, especially domestic animals, it is usually the result of ingesting moldy or fermented forage.Ascorbate Oxidase: An enzyme that converts ascorbic acid to dehydroascorbic acid. EC 1.10.3.3.Poaceae: A large family of narrow-leaved herbaceous grasses of the order Cyperales, subclass Commelinidae, class Liliopsida (monocotyledons). Food grains (EDIBLE GRAIN) come from members of this family. RHINITIS, ALLERGIC, SEASONAL can be induced by POLLEN of many of the grasses.Carbocysteine: A compound formed when iodoacetic acid reacts with sulfhydryl groups in proteins. It has been used as an anti-infective nasal spray with mucolytic and expectorant action.MycosesCephalosporins: A group of broad-spectrum antibiotics first isolated from the Mediterranean fungus ACREMONIUM. They contain the beta-lactam moiety thia-azabicyclo-octenecarboxylic acid also called 7-aminocephalosporanic acid.Ergotism: Poisoning caused by ingesting ergotized grain or by the misdirected or excessive use of ergot as a medicine.Ergotamines: A series of structurally-related alkaloids containing the ergotaman backbone structure.Hypocreales: An order of fungi in the phylum ASCOMYCOTA that includes a number of species which are parasitic on higher plants, insects, or fungi. Other species are saprotrophic.Penicillium chrysogenum: A mitosporic fungal species used in the production of penicillin.Norleucine: An unnatural amino acid that is used experimentally to study protein structure and function. It is structurally similar to METHIONINE, however it does not contain SULFUR.2-Aminoadipic Acid: A metabolite in the principal biochemical pathway of lysine. It antagonizes neuroexcitatory activity modulated by the glutamate receptor, N-METHYL-D-ASPARTATE; (NMDA).Fungi: A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.Gliocladium: A mitosporic fungal genus occurring in soil or decaying plant matter. It is structurally similar to Penicillium.Mycetoma: A chronic progressive subcutaneous infection caused by species of fungi (eumycetoma), or actinomycetes (actinomycetoma). It is characterized by tumefaction, abscesses, and tumor-like granules representing microcolonies of pathogens, such as MADURELLA fungi and bacteria ACTINOMYCETES, with different grain colors.Hyalohyphomycosis: OPPORTUNISTIC INFECTIONS caused by a heterogeneous group of MITOSPORIC FUNGI with clear (hyalo-) HYPHAE in the host. Common causative agents include ACREMONIUM; ASPERGILLUS; CHRYSOSPORIUM; FUSARIUM; PAECILOMYCES; PENICILLIUM; PSEUDALLESCHERIA; SCEDOSPORIUM; and SCOPULARIOPSIS. Normally a dermatomycoses, it can become invasive in the IMMUNOCOMPROMISED HOST.Musa: A plant genus of the family Musaceae, order Zingiberales, subclass Zingiberidae, class Liliopsida.Reproduction, Asexual: Reproduction without fusion of two types of cells, mostly found in ALGAE; FUNGI; and PLANTS. Asexual reproduction occurs in several ways, such as budding, fission, or splitting from "parent" cells. Only few groups of ANIMALS reproduce asexually or unisexually (PARTHENOGENESIS).Mitosporic Fungi: A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Intramolecular Transferases: Enzymes of the isomerase class that catalyze the transfer of acyl-, phospho-, amino- or other groups from one position within a molecule to another. EC 5.4.Molasses: The syrup remaining after sugar is crystallized out of SUGARCANE or sugar beet juice. It is also used in ANIMAL FEED, and in a fermented form, is used to make industrial ETHYL ALCOHOL and ALCOHOLIC BEVERAGES.Beta vulgaris: A species of the Beta genus. Cultivars are used as a source of beets (root) or chard (leaves).Technology, Pharmaceutical: The application of scientific knowledge or technology to pharmacy and the pharmaceutical industry. It includes methods, techniques, and instrumentation in the manufacture, preparation, compounding, dispensing, packaging, and storing of drugs and other preparations used in diagnostic and determinative procedures, and in the treatment of patients.Dimenhydrinate: A drug combination that contains diphenhydramine and theophylline. It is used for treating VERTIGO, MOTION SICKNESS, and NAUSEA associated with PREGNANCY.IranFermentation: Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.Soil: The unconsolidated mineral or organic matter on the surface of the earth that serves as a natural medium for the growth of land plants.Protoplasts: The protoplasm and plasma membrane of plant, fungal, bacterial or archaeon cells without the CELL WALL.Penicillins: A group of antibiotics that contain 6-aminopenicillanic acid with a side chain attached to the 6-amino group. The penicillin nucleus is the chief structural requirement for biological activity. The side-chain structure determines many of the antibacterial and pharmacological characteristics. (Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th ed, p1065)Aspergillus nidulans: A species of imperfect fungi from which the antibiotic nidulin is obtained. Its teleomorph is Emericella nidulans.Oxidoreductases: The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)Secondary Metabolism: A physiochemical process which occurs in a wide range of organisms which unlike BASAL METABOLISM is not required for or essential to short-term survivability but to long-term general well-being of the organism.Portal Vein: A short thick vein formed by union of the superior mesenteric vein and the splenic vein.Mycology: The study of the structure, growth, function, genetics, and reproduction of fungi, and MYCOSES.Biomedical Research: Research that involves the application of the natural sciences, especially biology and physiology, to medicine.Yeasts: A general term for single-celled rounded fungi that reproduce by budding. Brewers' and bakers' yeasts are SACCHAROMYCES CEREVISIAE; therapeutic dried yeast is YEAST, DRIED.Biomedical Engineering: Application of principles and practices of engineering science to biomedical research and health care.Natural Language Processing: Computer processing of a language with rules that reflect and describe current usage rather than prescribed usage.Magnetic Resonance Spectroscopy: Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).Antifungal Agents: Substances that destroy fungi by suppressing their ability to grow or reproduce. They differ from FUNGICIDES, INDUSTRIAL because they defend against fungi present in human or animal tissues.Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Peronospora: A genus of OOMYCETES in the family Peronosporaceae. Most species are obligatory parasites and many are plant pathogens.Fatigue: The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Systems Integration: The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Proteome: The protein complement of an organism coded for by its genome.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.

Contaminations occurring in fungal PCR assays. (1/240)

Successful in vitro amplification of fungal DNA in clinical specimens has been reported recently. In a collaboration among five European centers, the frequency and risk of contamination due to airborne spore inoculation or carryover contamination in fungal PCR were analyzed. The identities of all contaminants were specified by cycle sequencing and GenBank analysis. Twelve of 150 PCR assays that together included over 2,800 samples were found to be contaminated (3.3% of the negative controls were contaminated during the DNA extraction, and 4.7% of the PCR mixtures were contaminated during the amplification process). Contaminants were specified as Aspergillus fumigatus, Saccharomyces cerevisiae, and Acremonium spp. Further analysis showed that commercially available products like zymolyase powder or 10x PCR buffer may contain fungal DNA. In conclusion, the risk of contamination is not higher in fungal PCR assays than in other diagnostic PCR-based assays if general precautions are taken.  (+info)

Chemical modification of NADP-isocitrate dehydrogenase from Cephalosporium acremonium evidence of essential histidine and lysine groups at the active site. (2/240)

NADP-isocitrate dehydrogenase from Cephalosporium acremonium CW-19 has been inactivated by diethyl pyrocarbonate following a first-order process giving a second-order rate constant of 3.0 m-1. s-1 at pH 6.5 and 25 degrees C. The pH-inactivation rate data indicated the participation of a group with a pK value of 6.9. Quantifying the increase in absorbance at 240 nm showed that six histidine residues per subunit were modified during total inactivation, only one of which was essential for catalysis, and substrate protection analysis would seem to indicate its location at the substrate binding site. The enzyme was not inactivated by 5, 5'-dithiobis(2-nitrobenzoate), N-ethylmaleimide or iodoacetate, which would point to the absence of an essential reactive cysteine residue at the active site. Pyridoxal 5'-phosphate reversibly inactivated the enzyme at pH 7.7 and 5 degrees C, with enzyme activity declining to an equilibrium value within 15 min. The remaining activity depended on the modifier concentration up to about 2 mm. The kinetic analysis of inactivation and reactivation rate data is consistent with a reversible two-step inactivation mechanism with formation of a noncovalent enzyme-pyridoxal 5'-phosphate complex prior to Schiff base formation with a probable lysyl residue of the enzyme. The analysis of substrate protection shows the essential residue(s) to be at the active site of the enzyme and probably to be involved in catalysis.  (+info)

High performance liquid chromatography of natural products. I. Separation of cephalosporin C derivatives and cephalosporin antibiotics;isolation of cephalosporin C from fermentation broth. (3/240)

Microbonded propylamine silica with a solvent system containing acetic acid, methanol, acetonitrile and water (2:4:7.5:86.5) is suitable for an efficient separation of mixtures containing several closely related cephem derivatives. The same system with preparative columns was used for the isolation of cephalosporin C directly from the filtered broth of C. acremonium fermentation.  (+info)

RIT 2214, a new biosynthetic penicillin produced by a mutant of Cephalosporium acremonium. (4/240)

A number of lysine-requiring auxotrophs of Cephalosporium acremonium were investigated for incorporation of side-chain precursors and for accumulation of beta-lactam compounds. One of the auxotrophs, Acremonium chrysogenum ATCC 20389, producing cephalosporin C and penicillin N only if grown in media supplemented with DL-alpha-amino-adipic acid (DL-alpha-AAA), was found to use L-S-carboxymethylcysteine (L-CMC) as a side-chain precursor for the synthesis of a new penicillin (RIT 2214). No corresponding cephalosporin was detected. The penicillin present in the culture filtrate, was concentrated by adsorption on activated carbon and successive column chromatography on Amberlite IRA-68 and Amberlite XAD-4. Final purification was achieved by cellulose column chromatography. RIT 2214 was identified as 6-(D)-[(2-amino-2-carboxy)-ethylthio]-acetamido]-penicillanic acid by spectral analysis, bioactivity spectrum, elucidation of side-chain structure and finally by semisynthesis. Its biological properties were also evaluated.  (+info)

Fungi from geothermal soils in Yellowstone National Park. (5/240)

Geothermal soils near Amphitheater Springs in Yellowstone National Park were characterized by high temperatures (up to 70 degrees C), high heavy metal content, low pH values (down to pH 2.7), sparse vegetation, and limited organic carbon. From these soils we cultured 16 fungal species. Two of these species were thermophilic, and six were thermotolerant. We cultured only three of these species from nearby cool (0 to 22 degrees C) soils. Transect studies revealed that higher numbers of CFUs occurred in and below the root zone of the perennial plant Dichanthelium lanuginosum (hot springs panic grass). The dynamics of fungal CFUs in geothermal soil and nearby nongeothermal soil were investigated for 12 months by examining soil cores and in situ mesocosms. For all of the fungal species studied, the temperature of the soil from which the organisms were cultured corresponded with their optimum axenic growth temperature.  (+info)

Quantitative assessment of in planta distribution of metabolic activity and gene expression of an endophytic fungus. (6/240)

Using perennial ryegrass infected with an Acremonium transformant carrying the Escherichia coli beta-D-glucuronidase gene (gusA) (GUS system) under control of a constitutive promoter, we have developed methods for the quantitative extraction of endophyte-associated GUS activity from plant material. Fluorometric assays of these extracts allow quantitative assessment of the distribution of endophyte-associated GUS activity within single plants (tillers) with high resolution. Fluorescence microscopy with the dye Imagene Green can in addition visualize individual GUS-expressing hyphae. Since the transformant expresses the GUS gene constitutively, GUS activity can be used as an indicator of in planta endophyte metabolic activity. Using this approach we found that (i) the concentration of endophyte metabolic activity in plant tissue decreases with increasing plant size, (ii) approximately 70% of endophyte metabolic activity present in a plant is located in the leaf sheaths, (iii) basal-apical gradients and lateral (younger to older tissue) gradients of endophyte metabolic activity exist and (iv) basal-apical gradients are established early in leaf development. Our data suggest that the concentration of endophyte in each part of the plant is regulated so that a predetermined threshold of total endophyte activity per plant is not exceeded and a consistent distribution pattern is maintained.  (+info)

Influence of consumption of endophyte-infested tall fescue hay on performance of heifers and lambs. (7/240)

Two experiments were conducted to evaluate performance and physiological responses of heifers and lambs to Neotyphodium coenophialum-infested tall fescue hay fed under European rearing conditions. Endophyte-free (E-) or 100% endophyte-infested (E+) hay was derived from the same cultivar (cv. Clarine) so that the effect of the endophytic fungus could be clearly separated from a possible cultivar effect. In Exp. 1, starting in June 1996, 20 age- and body weight-paired Holstein dairy heifers were assigned for 97 d to one of two treatments consisting of ad libitum access to either E- or E+ hay, corresponding to 0 and .41 mg/kg ergovaline, respectively. During the experimental period, no significant difference (P>.20) in forage consumption, rectal temperature, or behavioral status of the animals was observed between the two treatments. The E+ diet induced a 10% apparent decrease in ADG and a clear reduction in prolactin (PRL) plasma concentration compared to the E- diet. When animals were all reassigned to a common endophyte-free diet, the E+ group recovered body weight and PRL to levels similar to those in animals fed E- after 7 wk. In Exp. 2, 30 Texel ram lambs were assigned to two treatments consisting of dietary E- or E+ tall fescue hay. The E- and E+ hays were harvested from the same plots as used in Exp. 1 and contained 0 and .96 mg/kg ergovaline, respectively. No effect of the endophyte was found on intake or carcass or testicle weight (P>.20) after the 95-d feeding period. The E+ treatment resulted in a slight reduction in BW at slaughter, mainly explained by a lower ruminal fill (P<.01). In E+ treated animals, prolactin concentrations dropped significantly (P<.001) from d 27. Hay assessment in both experiments showed no difference in chemical composition and IVDMD. The endophytic fungus strongly lowered the palatability of the E+ hay, although there was no effect on intake with heifers (Exp. 1) or with lambs (Exp. 2). The potential of severe heat stress, as expressed by the temperature humidity index, was not high in our experimental conditions, although they were considered rather unusually stressful for the western part of northern Europe. Yet, no economic effect on cattle was observed, in disagreement with results obtained in many previous U.S. studies.  (+info)

The fungal CPCR1 protein, which binds specifically to beta-lactam biosynthesis genes, is related to human regulatory factor X transcription factors. (8/240)

Here we report the isolation and characterization of a novel transcription factor from the cephalosporin C-producing fungus Acremonium chrysogenum. We have identified a protein binding site in the promoter of the beta-lactam biosynthesis gene pcbC, located 418 nucleotides upstream of the translational start. Using the yeast one-hybrid system, we succeeded in isolating a cDNA clone encoding a polypeptide, which binds specifically to the pcbC promoter. The polypeptid shows significant sequence homology to human transcription factors of the regulatory factor X (RFX) family and was designated CPCR1. A high degree of CPCR1 binding specificity was observed in in vivo and in vitro experiments using mutated versions of the DNA binding site. The A. chrysogenum RFX protein CPCR1 recognizes an imperfect palindrome, which resembles binding sites of human RFX transcription factors. One- and two-hybrid experiments with truncated versions of CPCR1 showed that the protein forms a DNA binding homodimer. Nondenaturing electrophoresis revealed that the CPCR1 protein exists in vitro solely in a multimeric, probably dimeric, state. Finally, we isolated a homologue of the cpcR1 gene from the penicillin-producing fungus Penicillium chrysogenum and determined about 60% identical amino acid residues in the DNA binding domain of both fungal RFX proteins, which show an overall amino acid sequence identity of 29%.  (+info)

Creation of isopenicillin N from δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine (ACV) in the penicillin and cephalosporin biosynthetic pathway is catalysed by isopenicillin N synthase (IPNS), a non-heme iron-containing dioxygenase. A tripeptide R-X-S motif which consists of arginine-281 and serine-283 (Cephalosporium acremonium IPNS numbering) was found to be conserved in IPNS and other related proteins. These two amino acids mentioned were proposed to have a role in ACV substrate binding by the recent Aspergillus nidulans IPNS crystal structure. Using site-directed mutagenesis, arginine-281 in C. acremonium IPNS (cIPNS) was earlier found to be essential for catalysis by our group. Similarly, serine-283 in cIPNS was also altered by site-directed mutagenesis to determine its role in cIPNS. No measurable activity was detected from the resultant mutant using enzyme bioassays. It is most likely that the elimination of the mutants substrate-binding capability similar to that of arginine-281 lead to the ...
Fulltext - Production of Cephalosporin C by Acremonium chrysogenum Grown on Beet Molasses: Optimization of Process Parameters Through Statistical Experimental Designs
Bioassay-guided fractionation of metabolites from the fungus Cephalosporium sp.AL031 isolated from Sinarundinaria nitida led to the discovery of a new isobenzofuranone derivative, 4,6-dihydroxy-5-methoxy-7-methylphthalide (1), together with three known compounds: 4,5,6-trihydroxy-7-methyl-1,3-dihydroisobenzofuran (2), 4,6-dihydroxy-5-methoxy-7-methyl-1,3-dihydroisobenzofuran (3) and 4,5,6-trihydroxy-7-methylphthalide (4). The structure of the new compound 1 was determined based on MS, 1D and 2D NMR spectral data. Compounds 1-4 showed potent antioxidant activity with EC50 values of 10, 7, 22 and 5 μM by 1,1-diphenyl-2-picryhydrazyl (DPPH) radical-scavenging assay.
Cephalosporin C (CPC) is a major precursor of cephalosporin antibiotics which is produced by a group of deuteromycets. After biosynthesis in optimized fermentation media, CPC is recovered and converted to a variety of potent cephalosporin antibiotic drugs. This study was performed to determine the mycoflora producing CPC in various parts of Iran soil. Soil samples were collected and cultured on selective media and Acremonium sp. were isolated. The presence of CPC antibiotic in the fermentation broths of these species was determined by a combination of biological, HPLC and mass spectrometry methods. From 350 fungi isolated, 21 (6%) were Acremonium spp, which one strain was able to produce CPC. This species was recognized as Acremonium persicinum according to its macroscopic and microscopic criteria. It is possible to apply different characteristics of the isolated species in strain optimization processes such as protoplast fusion ...
Two new benzophenones, acredinones A (1) and B (2), were isolated from a marine-sponge-associated Acremonium sp. fungus. Their chemical structures were elucidated on the interpretation of spectroscopic data. The structure of 1 was confirmed by palladium-catalyzed hydrogenation, followed by spectroscopic data analysis. Acredinones A (1) and B (2) inhibited the outward K+ currents of the insulin secreting cell line INS-1 with IC50 values of 0.59 and 1.0 mu M, respectively ...
Coloured scanning electron micrograph (SEM) of Acremonium stromaticum, a hyaline hyphomycete (glassy, lightly pigmented conidial fungus) that occurs widely in nature but is also encountered as a contaminant. Asexual reproduction is by conidiophores that produce conidia (phialoconidia or ameroconidia) at hyphal branches. A. stromaticum is one of the causative agents of eumycotic white grain mycetoma. It is also one of five fungi present in most banana root and rhizome lesions in many parts of the tropics. A. stromaticum is known to cause opportunistic infections (hyalohyphomycosis) in immunocompromised patients, including arthritis, osteoarthritis, peritonitis, endocarditis, pneumonia, cerebritis and subcutaneous infection. Magnification: x1,200 when shortest axis printed at 25 millimetres. - Stock Image C032/2959
Microorganisms are the most proficient decomposers in nature, using secreted enzymes in the hydrolysis of lignocellulose. As such, they present the most abundant source for discovery of new enzymes. Acremonium alcalophilum is the only known cellulolytic fungus that thrives in alkaline conditions and can be cultured readily in the laboratory. Its optimal conditions for growth are 30°C and pH 9.0-9.2. The genome sequence of Acremonium alcalophilum has revealed a large number of genes encoding biomass-degrading enzymes. Among these enzymes, lipases are interesting because of several industrial applications including biofuels, detergent, food processing and textile industries. We identified a lipA gene in the genome sequence of Acremonium alcalophilum, encoding a protein with a predicted lipase domain with weak sequence identity to characterized enzymes. Unusually, the predicted lipase displays ≈ 30% amino acid sequence identity to both feruloyl esterase and lipase of Aspergillus niger. LipA, when
Cephalosporium stripe, caused by the soil-borne ascomycete Cephalosporium gramineum, is becoming an increasingly important disease of winter wheat (Triticum aestivum) in several areas of the world, especially where stubble mulch is practiced to maintain soil moisture and prevent erosion. As cultural control of the disease is infeasible and no fungicides are registered, the development of resistant cultivars offers the best hope for disease control. Selection of resistant genotypes remains problematic due to the requirements of evaluating adult plants in variable field environments. The symptoms of cephalosporium stripe suggest the involvement of pathogen-produced toxins, and the toxin called graminin A has previously been isolated from C. gramineum. The goals of this thesis were to determine if insensitivity of wheat genotypes to a toxic fraction produced by C. gramineum is associated with resistance to cephalosporium stripe, and to evaluate the potential use of this toxic fraction to screen ...
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Isopenicillin N synthase (IPNS) catalyses a key step in the penicillin and cephalosporin biosynthetic pathway which involves the oxidative cyclisation of the acyclic peptide δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine (ACV) to isopenicillin N. Based on crystallographic evidence from the Aspergillus nidulans IPNS crystal structure complexed with the substrate ACV (Roach et al. (1997) Nature 387, 827-830), we were able to provide mutational evidence for the critical involvement of the conserved R-X-S motif in ACV binding in IPNS. The crystal structure further implicated arginine-87 in the binding of the aminoadipyl portion of ACV. Thus, in this study, the site-directed mutagenesis of the corresponding arginine-89 in Cephalosporium acremonium IPNS (cIPNS) was performed to ascertain its role in cIPNS. Alteration of arginine-89 to five amino acids from different amino acid groups, namely lysine, serine, alanine, aspartate and leucine, was performed and no activity was detected in all the mutants ...
菌生真菌為一種真菌分別以寄生、片利共生、腐生的形式生長在另一種真菌上,目前仍無法明確得知其菌生菌與宿主間的相互作用。子囊菌門當中的肉座菌目(Hypocreales),有很大一部分為菌生真菌的種類。本研究以隨機採集的方式,鑑定及分析北台灣菌生真菌種類,同時記錄台灣新的真菌種類。利用真菌型態學及DNA分子技術鑑定出真菌種類;比較真菌培養於玉米瓊酯培養基及自然基質下,真菌型態的不同,同時利用Internal transcribed spacer (ITS),Large subunit (LSU) rDNA或 small subunit (SSU) rDNA 鑑定真菌種類。此研究採集及鑑定的菌生真菌有14種,分別是: Acremonium sp., Acremonium citrinum, Cladobotryum semicirculare, Hypomyces chrysospermus, Hypomyces albidus, Hypomyces corticiicola, Lecancillium psallioate, Nectriopsis rexiana‏, Ramularia coleosporii, Ramularia sp.?, Simplicillium lanosoniveum, Sphaerostilbella sp., Trichoderma ...
Penicillins (Penams) and cephalosporins (cephems) are beta-lactam antibiotics containing 6-aminopenicillanate (6-APA) and 7-aminocephalosporanate (7-ACA) nuclei, respectively. 6-APA and 7-ACA are key intermediates for a variety of semisynthetic penicillin and cephalosporin derivatives. Penicillins are produced only by fungi, while cephalosporins (including cephamycins) are produced by fungi and bacteria. Both antibiotics are synthesized from L-2-aminoadipate, L-cysteine and L-valine through a common pathway. It starts with the condensation of these three amino acids by the non-ribosomal peptide synthetase to form the tripeptide delta-(L-2-aminoadipyl)-L-cysteinyl-D-valine (ACV). The linear ACV tripeptide is then converted to bicyclic isopenicillin N by isopenicillin N synthase, in which the beta-lactam ring is formed. Isopenicillin N is the branch point of penicillin [MD:M00672] and cephalosporin [MD:M00673] pathways ...
Isopenicillin N synthase (IPNS) is a non-heme iron-dependent enzyme belonging to the oxidoreductase family. This enzyme catalyzes the formation of isopenicillin N from δ-(L-α-aminoadipoyl)-L-cysteinyl-D-valine (LLD-ACV). N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 ⇌ {\displaystyle \rightleftharpoons } isopenicillin N + 2 H2O This reaction is a key step in the biosynthesis of penicillin and cephalosporin antibiotics. The active sites of most isopenicillin N synthases contain an iron ion. This enzyme is also called isopenicillin N synthetase. A Fe(II) metal ion in the active site of the enzyme is coordinated by at least two histidine residues, an aspartate residue, a glutamine residue, and two water molecules in the absence of a bound substrate. Just two histidine residues and one aspartic acid residue are entirely conserved. Therefore, it is highly significant that these two histidine residues, His214 and His270, and one aspartic acid residue, Asp216, are precisely the ones ...
Mycocosm Portal version:12.104 jgi-myco-web-2.nersc.gov Release Date:30-Jul-2020 15:17:52 PST Current Date:09-Aug-2020 23:02:43.754 PDT ...
en] A strain of Acremonium luzulae (Fuckel) W. Gams was selected in screening new microorganisms for biological control of fruit postharvest diseases, especially gray and blue mold diseases on apples and strawberries. This strain manifests a very strong activity against a large number of phytopathogenic fungi. In this work, the product responsible for this antifungal activity was isolated from modified Sabouraud dextrose broth cultures of A. luzulae. It was purified to homogeneity by reverse-phase column chromatography. On the basis of UV, infrared, and 1H and 13C nuclear magnetic resonance spectra, mass spectral analysis, and the amino acid composition of the acid hydrolysates, the antibiotic was determined to be cyclosporin C. Cyclosporin C showed a broad-spectrum activity against filamentous phytopathogenic fungi but no activity against bacteria or yeasts. Its antifungal activity is only fungistatic. In contrast to Tolypocladium inflatum, another cyclosporin-producing strain, A. luzulae, did ...
Fungi exist widely in different environments, such as soil, biological wastes and plants. Some of them have been utilized by humans for over 1000 years. In nature, fungi play a vital role in numerous degradation processes. In agriculture, many species of fungi are used for control of plant pests and diseases [1, 2]. In medicine, fungi are utilized to produce antibiotics for the treatment of diseases. For example, penicillin is a product of Penicillium chrysogenum and cephalosporin of Cephalosporium acremonium. Due to their good capacity in manufacturing valuable proteins and secondary metabolites, fungi are important economic contributors. With the explosion and exploration of fungal genomic sequence information, mycology is coming into a new era of functional studies [3].. Filamentous fungi, molds, grow well and rapidly on simple and inexpensive media and thus are preferred cell factories due to their outstanding capacity in expression and secretion of heterologous proteins with ...
? Caecum strictum De Folin , 1871, moliuskų (Mollusca) tipo pilvakojų (Gastropoda) klasės (Littorinimorpha) būrio (Caecidae) šeimos (Caecum) genties rūšis. Turinys[rodyti] Išvaizda [ k ] Biologija Mityba Paplitimas [ k ] Literatūra Bouchet, P.; Rosenberg, G. (2010). Caecum strictum de Folin...
57) Abstract:. The invention relates to the field of biotechnology and relates to a method of biosynthesis of cephalosporin C in liquid nutrient medium with the use of culture Acremonium chrysogenum. The proposed method includes the cultivation of a producer in a liquid nutrient medium containing sources of carbon, nitrogen, mineral salts, vegetable oil under conditions of aeration and mixing, regulating the concentration of ammonia nitrogen and pH of the culture fluid. As a source of polysaccharide nutrient medium contains hydrolyzed corn or potato starch, obtained by treating starch enzyme complex drug Miloserdiya with a specific activity of 600-3000 U/g Mass fraction of starch hydrolysate in the medium - 8-10% based on the starch. The process of biosynthesis lead during the first 30 hours at a temperature of 28-29°C, followed by a change in temperature and the process of biosynthesis at 24-25°C. the Process of antibioticaccutane ends at 135-140 h of growth activity in the culture fluid ...
The objective of the study was to examine fetal growth during maternal exposure to ergot alkaloids during gestation. Pregnant Southdown ewes (n=16) were randomly assigned to one of two dietary treatments: 1) endophyte infected tall fescue seed (E+) and 2) endophyte-free tall fescue seed (E-). The fescue seed was delivered daily in a total mixed ration. The seed compromised 38.5% of the ration.The fescue seed was fed to simlulate the fescue toxicosis syndrome during gestation (d 35 to parturition ...
Citation: Bacetty, A.A., Snook, M.E., Glenn, A.E., Bacon, C.W., Schardl, C.L., Nagabhyru, P. 2007. Nematoxic effects of endophyte-infected tall fescue toxins and extracts to an in vitro bioassay using the nematode Pratylenchus scribneri. Acremonium Grass Interactions International Symposium Proceedings. March 25-28, 2007. Christchurch, New Zealand. Interpretive Summary: Abstract - no summary required Technical Abstract: Biotypes of Neotyphodium-tall fescue grass symbiota are provided with enhanced protection from grazing vertebrate herbivores and insects due to the production of toxic secondary metabolites. However, considerable controversy exists concerning the involvement of the Neotyphodium coenophialum and tall fescue symbiotum and toxicity to nematode species. A sterile in vitro system was developed to determine the interactive nature of known toxins specific to this mutualistic association and compounds known to be nematotoxic. The in vitro assay system developed used Pratylenchus ...
Citation: Bischoff, K.M., De Rezende, S.T., Larson, T.M., Liu, S., Hughes, S.R., Rich, J.O. 2011. Purification and characterization of arabinofuranosidase from the corn endophyte Acremonium zeae. Biotechnology Letters. 33(10):2013-2018. DOI: 10.1007/s10529-011-0658-9. Interpretive Summary: In this research we discovered two enzymes that are involved in releasing sugar from lignocellulosic biomass. New enzymes that can degrade cellulose and hemicellulose are needed to help overcome some of the technical barriers to using agricultural residues as feedstocks for fuel ethanol production. Acremonium zeae is a fungus that was found to produce two forms of an arabinofuranosidase enzyme. A mixture of both forms of the enzyme could release high percentages of the sugars arabinose and xylose from corn fiber and wheat hemicellulose. Results will be valuable to researchers developing new enzymes to serve as biocatalysts in the conversion of agricultural residues to fermentable sugars. Technical Abstract: ...
37 different modules for Arduino Compatible (list is given below) Best kit for learning Arduino Material: Circuit board Package Dimensions: 273*190*43mm Weight: 431g. 1 x Small passive buzzer module KY-006 1 x 2-color LED module KY-011 1 x Hit sensor module KY-031 1 x Vibration switch module KY-002 1 x Photo resistor module KY-018 1 x Key switch module KY-004 1 x Tilt switch module KY-020 1 x 3-color full-color LED SMD modules KY-009 1 x Infrared emission sensor module KY-005 1 x 3-color LED module KY-016 1 x Mercury open optical module KY-017 1 x Yin Yi 2-color LED module 3MM KY-029 1 x Active buzzer module KY-012 1 x Temperature sensor module KY-013 1 x Automatic flashing colorful LED module KY-034 1 x Mini magnetic reed modules KY-021 1 x Hall magnetic sensor module KY-003 1 x Infrared sensor receiver module KY-022 1 x Class Bihor magnetic sensor KY-035 1 x Magic light cup module KY-027 1 x Rotary encoder module KY-040 1 x Optical broken module KY-010 1 x Detect the heartbeat module KY-039 1 ...
Brendemuehl JP, Williams MA, Boosinger TR, et al. 1995. Plasma progestagen, tri-iodothryonine, and cortisol concentrations in postdate gestation foals exposed in utero to the tall fescue Acremonium coenophialum, pp 53-59. In Sharp DC, Bazer FW (eds), Equine reproduction, ed 6. Society for the Study of Reproduction, Madison, WI ...
A variety of antibiotic-producing microbes are currently available at ATCC. These include strains from the bacterial and fungal genera |em>Streptomyces|/em>, |em>Penicillium|/em>, and |em>Acremonium|/em>.
A variety of antibiotic-producing microbes are currently available at ATCC. These include strains from the bacterial and fungal genera |em>Streptomyces|/em>, |em>Penicillium|/em>, and |em>Acremonium|/em>.
Indikasjoner:Arthritis urica med eller uten knuter. I kombinasjon med penicillin når høye kontinuerlige serumkonsentrasjoner er ønskelig.
Aceptado 11 de octubre 2008.. Resumen. El herbicida isoproturon (IPU) es un contaminante fuerte debido a su elevada solubilidad acuosa y bajo potencial para la degradación microbiana. El problema ambiental se debe a su amplio empleo en la agricultura convencional, áreas urbanas, algicida y aditivo de pinturas. Especies fúngicas degradaron el herbicida en cultivos in vitro, produciendo derivados hidroxilados frecuentes en areas agrícolas. Los objetivos planteados fueron aislar las especies fúngicas representativas de suelos tratados con IPU, evaluar su potencial transformador, e identificar los derivados. La mayoría de los aislamientos produjeron 1 OH IPU y 2 OH IPU, indicando que los hongos pueden ser la causa de estos compuestos en las muestras ambientales. De los 35 aislamientos, 10 de ellos fueron especies dominantes y activas degradadoras. Aspergillus ochraceus, Fusarium flocciferum, Talaromyces helicus, Acremonium strictum, Mucor hiemalis, Paecilomyces lilacinus y Penicillium ...
ID ACVS_AMYLA Reviewed; 3649 AA. AC P27743; DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot. DT 01-AUG-1992, sequence version 1. DT 25-OCT-2017, entry version 96. DE RecName: Full=N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; DE EC=6.3.2.26; DE AltName: Full=Delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase; DE Short=ACV synthetase; DE Short=ACVS; GN Name=pcbAB; OS Amycolatopsis lactamdurans (Nocardia lactamdurans). OC Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae; OC Amycolatopsis. OX NCBI_TaxID=1913; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=VAR LC 411; RX PubMed=1956290; DOI=10.1111/j.1365-2958.1991.tb01885.x; RA Coque J.J.R., Martin J.F., Calzada J.G., Liras P.; RT "The cephamycin biosynthetic genes pcbAB, encoding a large multidomain RT peptide synthetase, and pcbC of Nocardia lactamdurans are clustered RT together in an organization different from the same genes in RT Acremonium chrysogenum and Penicillium chrysogenum."; RL Mol. ...
Three exocellular beta-1,3-glucanases from Acremonium blochii strain C59, BGN3.2, BGN3.3 and BGN3.4, were purified. Two, BGN3.2 and BGN3.4 appeared to act as exo-enzymes against laminarin from Laminaria digitata, while BGN3.3 displayed an endo-mode of action. The N-terminal amino acid sequence data for BGN3.2 and BGN3.4 suggested these two enzymes may be encoded by different genes. The gene encoding the BGN3.2 glucanase was fully sequenced, and its deduced amino acid sequence was similar to those for all other sequenced fungal exo-beta-1,3-glucanases. This BGN3.2 gene consists of an uninterrupted ORF of 2349 bp encoding 783 amino acids possibly with two cleavage sites for the potential removal of a pre- and pro-protein, respectively. A DNA fragment encoding a portion of the BGN3.4 gene was amplified by PCR, and the nucleotide sequence of this fragment confirmed that BGN3.2 and BGN3.4 are encoded by different genes. The internal peptide sequences of BGN3.3 were not present in the amino acid ...
10. Nov. 2015 Doctoral - Diploma - Bachelor - Master Thesis . of clubroot (Plasmodiophora brassicae) by the endophytic fungus Acremonium alternatum.
Lee, H. J., Schofield, C. J. and Lloyd, M. D., 2002. Active site mutations of recombinant deacetoxycephalosporin C synthase. Biochemical and Biophysical Research Communications, 292, pp. 66-70.. ...
TABLE-US-00001 TABLE 1 Cultural characteristics of Acremonium persicinum strain MF-347833 Media Cultural characteristics Malt extract agar Growth: Rapidly. 30-31 mm in diameter. Surface: Circular, undulate at margin, floccose, white (1A1). Reverse: Pale yellow to pale orange (5A3). Potato dextrose agar Growth: Rapidly. 39-41 mm in (Difco 2010) diameter. Surface: Circular, undulate at margin, floccose, white (1A1) to yellowish white (4A2). Reverse: Sulcate, ivory (4A3). Mustard brown at center (5E6). Czapek solution agar Growth: Rapidly. 57-59 mm in diameter. Surface: Circular, entire at margin. Felty, somewhat reddish gray at center, generally white (1A1). Reverse: Pale orange (5A2). Sabouraud dextrose Growth: Rapidly. 32-33 mm in agar (Difco 0109) diameter. Surface: Circular, undulate at margin. Forming striates. Floccose, white (1A1). Reverse: Sulcate, yellowish white (4A2). Emerson YpSs agar Growth: Rapidly. 36-38 mm in (Difco 0739) diameter. Surface: Circular, undulate at margin. Felty, ...
Shop Isopenicillin N epimerase component ELISA Kit, Recombinant Protein and Isopenicillin N epimerase component Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Preparations of the enzyme isopenicillin N synthetase from CO 728 convert the modified tripeptide substrate δ-(-α-aminoadipoyl)--cysteingyl--allylgycine into penam, homoceph-3-em, hydroxycepham, and hydroxyhomocepham type products; these have been isolated and their structures established.
1OBN: Structural Studies on the Reaction of Isopenicillin N Synthase with the Substrate Analogue Delta-(L-Alpha-Aminoadipoyl)-L-Cysteinyl-D-Alpha-Aminobutyrate.
1OBN: Structural Studies on the Reaction of Isopenicillin N Synthase with the Substrate Analogue Delta-(L-Alpha-Aminoadipoyl)-L-Cysteinyl-D-Alpha-Aminobutyrate.
Created page with {{header_links}} : Myceliophthora :Acremonium alcalophilum : Amorphotheca resinae : Calcarisporiella thermophila : Corynascus thermophilus : Gloeophyllum trabeum : Letinula edo...) ...
Ecological Characteristics of White Stain Symptom on the Grape in Korea - Acremonium acutatum;Ecology;Grape;Trichothecium roseum;White stain symptom;
Mr Rhidian Morgan-Jones, Consultant Orthopaedic Surgeon, MB BCh, FRCS, MMedSci, FRCS (Trauma & Orthopaedics) at Spire Healthcare. Learn more about this consultant here.
SWISS-MODEL Template Library (SMTL) entry for 1hjf.1. Alteration of the co-substrate selectivity of deacetoxycephalosporin C synthase: The role of arginine-258
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GONZALEZ-PEREZ, Enrique; YANEZ-MORALES, María de Jesús; ORTEGA-ESCOBAR, Héctor Manuel y VELAZQUEZ-MENDOZA, Juan. Comparative Analysis among Pathogenic Fungal Species that Cause Gladiolus (Gladiolus grandiflorus Hort.) Corm Rot in Mexico. Rev. mex. fitopatol [online]. 2009, vol.27, n.1, pp.45-52. ISSN 2007-8080.. An unknown disease causing wilting and death of gladiolus plants was studied in 2004, in Tlacotepec de José Manzo, in the municipality of San Salvador el Verde, located in the region of San Martín Texmelucan, Puebla, Mexico. The objective was to identify and characterize by ITS-PCR the plant pathogen(s) that cause this disease and to comparatively analyze the fungal species involved. Five species of fungi were identified from healthy and symptomatic corms taken in the field and from storage, and also from damaged basal stem and neck tissues of field plants, these were: Fusarium oxysporum f. gladioli, F. solani, Penicillium sp.; and two previously reported Acremonium strictum and ...
Sorbicillinoids are a diverse group of yellow secondary metabolites that are produced by a range of not closely related ascomycetes, including Penicillium chrysogenum, Acremonium chrysogenum, and Trichoderma reesei. They share a similarity to the name-giving compound sorbicillin, a hexaketide. Previously, a conserved gene cluster containing two polyketide synthases has been identified as the source of sorbicillin, and a model for the biosynthesis of sorbicillin in P. chrysogenum has been proposed. In this study, we deleted the major genes of interest of the cluster in T. reesei, namely sor1, sor3, and sor4. Sor1 is the homolog of P. chrysogenum SorA, which is the first polyketide synthase of the proposed biosynthesis pathway. Sor3 is a flavin adenine dinucleotide (FAD)-dependent monooxygenase, and its homolog in P. chrysogenum, SorC, was shown to oxidize sorbicillin and 2,3-dihydrosorbicillin to sorbicillinol and 2,3-dihydrosorbicillinol, respectively, in vitro. Sor4 is an FAD/flavin ...
1. Penicillin N was synthesized by coupling alpha-amino-alpha-p-nitrobenzyl-N-p-nitro-benzyloxycarbonyl-D-adipate with 6-aminopenicillanic acid benzyl ester, followed by removal of the protecting groups through hydrogenolysis. 2. alpha-Amino-alpha-p-nitrobenzyl-N-p-nitrobenzyloxycarbonyl-D-[5-14C]adipate was prepared by treating alpha-p-nitrobenzyl-N-p-nitrobenzyloxycarbonyl-D-glutamic acid with [14C]diazomethane followed by rearrangement with silver trifluoromethanesulphonate. 3. Coupling of alpha-amino-alpha-p-nitrobenzyl-N-p-nitrobenzyloxycarbonyl-D-[5-14C]adipate with 6-aminopenicillanic acid benzyl ester gave triprotected [10-14C]penicillin N. 4. 3H was introduced at C-6 of the Schiffs base derivative (10) by oxidation followed by reduction with NaB3H4. 5. The so-derived (6 alpha-3H)-labelled Schiffs base was hydrolysed to give 6-amino [6 alpha-3H]penicillanic acid benzyl ester p-toluenesulphonic acid salt, which after coupling as the free amine with ...
Tall fescue toxicosis plagues many producers who graze cattle throughout the southeastern United States. Previous studies have shown that Ivermactin dewormer has reduced fescue toxicosis symptoms, however ivermectin only acts for roughly 28 days. This study used eprinomectin, commercially known as LongRange,a drug in the same family (avermactins) as ivermectin but that lasts for 100-150 days to see if this also reduced signs of fescue toxicosis ...
Tall fescue ( Festuca arundinaceaSchreb.) is a major cool season forage and turf grass in the temperate regions of the world. It is also a close relative of other important forage and turf grasses,...
MOLD AND LYME TOXINS. Richard Loyd, Ph.D.. Mold toxins are found in foods such as grains and peanuts. Far more serious are the mold toxins found in buildings and vehicles with water leaks. Buildings with flat roofs and buildings on a concrete slab are ready-made for mold problems. Mold toxins are the most common of the biotoxins and are responsible for many if not most of the symptoms of many Lyme disease patients.. When a building has a water leak or water damage that is not immediately corrected, molds such as Stachybotrys, Aspergillus, Acremonium, Actiniomycetes, Penicillium and Chaetomium are likely to start colonies. The colonies may be visible or they may be hidden in places such as the tops of ceiling tiles or the bottoms of carpets. The molds send out spores which contain powerful biotoxins that are nerve toxins or neurotoxins. A study by Harvard University found that fully half of the homes studied had enough mold to cause symptoms. And we have found that many cars are ...
16117DNAArtificial SequenceSequence of the oligonucleotide primer CBH_1S 1tggggncart gyggngg 17217DNAArtificial SequenceSequence of the oligonucleotide primer CBH_1AS 2gcnggccanc cnarcca 17321DNAArtificial SequenceSequence of the oligonucleotide primer CBH_8 3atggctaagc agctgctgct c 21420DNAArtificial SequenceSequence of the oligonucleotide primer CBH_9 4tcagarcgga gggttggcat 20544DNAArtificial SequenceSequence of the oligonucleotide primer Te_CBH_A 5tattatccgc ggactgcgca tcatgcggaa tcttcttgct cttg 44638DNAArtificial SequenceSequence of the oligonucleotide primer Te_CBH_B 6aatttggatc ctcagaacag cgggttagca ttcgtgag 3871014DNAArtificial SequenceSequence of the PCR fragment obtained from Acremonium thermophilum ALKO4245 (CBS 116240) using the primers CBH_1S and CBH_1AS 7tggggacaat gcggcggcaa cggcttctcg ggaccgacct gttgcacgtc gggcaacacc 60tgcgtcaagc tcaatgactg gtactcccag tgcctgccca acagccaggt acgctgcgcc 120cagccgctct agctggactt ctccttttac gagatgtctt ggctgacctc cttccaaagg 180tgaccaccac cacaagctcg ...
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For producers with vast acres of toxic tall fescue, there are few more daunting tasks than eradicating the endophyte-ridden forage.It takes time, money, and patience, but help is here. The Alliance fo,Forage Flash, Workshops offer a fescue fix
John F. Peberdy (1987). Penicillium and Acremonium. Springer Science & Business Media. ISBN 0306423456. Visagie, C. M.; ...
John F. Peberdy (2013). Penicillium and Acremonium. Springer Science & Business Media. ISBN 1489919864. John I. Pitt (1979). ...
doi:10.1016/S0007-1536(70)80016-X. John F. Peberdy (1987). Penicillium and Acremonium. Springer Science & Business Media. ISBN ...
John F. Peberdy (1987). Penicillium and Acremonium. Springer Science & Business Media. ISBN 0306423456. ...
ISBN 3-13-179282-5. John F. Peberdy (2013). Penicillium and Acremonium. Springer Science & Business Media. ISBN 1-4899-1986-4. ...
John F. Peberdy (1987). Penicillium and Acremonium. Springer Science & Business Media. ISBN 0306423456. Q. Ashton Acton (2013 ...
Penicillium and Acremonium. Springer Science & Business Media. ISBN 0306423456. ...
Peberdy, John F (1987). Penicillium and Acremonium. New York: Plenum Press. ISBN 0306423456. Georghiou, G. P. (2012). Pest ...
John F. Peberdy (1987). Penicillium and Acremonium. Springer Science & Business Media. ISBN 0306423456. List of Penicillium ...
John F. Peberdy (2013). Penicillium and Acremonium. Springer Science & Business Media. ISBN 1489919864. List of Penicillium ...
John F. Peberdy (2013). Penicillium and Acremonium. Springer Science & Business Media. ISBN 1489919864. V. Betina (1993). ...
John F. Peberdy (2013). Penicillium and Acremonium. Springer Science & Business Media. ISBN 9058231593. List of Penicillium ...
ISBN 0-203-90910-0. John F. Peberdy (2013). Penicillium and Acremonium. Springer Science & Business Media. ISBN 1-4899-1986-4. ...
John F. Peberdy (2013). Penicillium and Acremonium. Springer Science & Business Media. ISBN 1489919864. John I. Pitt, Ailsa D. ...
of the order microascales). The mitosporic Acremonium spp. degrade PAhs and Royal Demolition Explosive (RDX), and Graphium spp ...
Acremonium/grass interactions: plenary papers. (Hume, D.E.; Latch, G.C.M. & Easton, H.S., eds.) Palmerston North, NZ: ... West, C.P. & Gwinn, K.D. (1993). Role of Acremonium in drought, pest and disease tolerance of grasses. In: Proc. 2nd Int. Symp ... Acremonium/Grass interactions (Quisenberry, S.S. & Joost, R.E., eds.) Baton Rouge: Louisiana Agriculture Experiment Station. ... West, C.P.; Izekor, E.; Oosterhuis, D.M.; Robbins, R.T. (1988). "The effect of Acremonium coenophialum on the growth and ...
Curvularia Acremonium Yeasts Candida Fusarium spp. is most common then Aspergillus spp. and thirdly Dematitious fungi causing ...
Ceccherelli P, Fringuelli R, Madruzza GF (1975). "Cerevisterol and ergosterol peroxide from Acremonium luzulae". Phytochemistry ... Acremonium luzulae, and Pencillium herquei, as well as the lichens Ramalina hierrensis and Stereocaulon azoreum. It has also ...
These include the cephalosporin producing Acremonium chrysogenum. Geldanamycin is produced by Streptomyces hygroscopicus. ...
A fungus of the genus Cephalosporium Corda (now been renamed Acremonium strictum) was able to decrease the dissemination of ... "Mycoparasitism of Helminthosporium solani by Acremonium strictum". Phytopathology. 97 (10): 1331-1337. doi:10.1094/PHYTO-97-10- ...
An endophyte of Festuca arundinacea and the anamorph of Epichloe typhina, new taxa in one of two new sections of Acremonium". ... Glenn AE, Bacon CW, Price R, Hanlin RT (1996). "Molecular phylogeny of Acremonium and its taxonomic implications". Mycologia. ... of genus Acremonium. In a molecular phylogenetic study in 1996, Glenn and colleagues found that genus to be polyphyletic and ...
Several species of endophytic fungi, especially Acremonium spp., have also been studied with a view to biological control in ... however Acremonium lolii renders perennial ryegrass toxic to livestock at some stages of growth, putting them at risk of ...
... (3) was independently isolated from Acremonium sp. TF-0356 and was assigned the trivial name NGA0187. Nozawa Y, Sakai ... is a naturally occurring ergostane or lanostane steroid produced by Acremonium sp. TF-0356 which has nerve growth factor-like ...
An endophyte of Festuca arundinacea and the anamorph of Epichloe typhina, new taxa in one of two new sections of Acremonium". ... Glenn AE, Bacon CW, Price R, Hanlin RT (1996). "Molecular phylogeny of Acremonium and its taxonomic implications". Mycologia. ... initially in the genus Acremonium, and after 1996, in the new genus Neotyphodium. Since 2011, the nomenclatural code has ...
The genus is intermediate between Acremonium and Phialophora. Gams W, McGinnis MR. (1983). "Phialemonium, a new anamorph genus ... intermediate between Phialophora and Acremonium". Mycologia. 75 (6): 977-87. doi:10.2307/3792653. Phialemonium in Index ...
Acremonium acutatum Acremonium alabamense Acremonium alcalophilum Acremonium alternatum Acremonium antarcticum Acremonium apii ... Acremonium arxii Acremonium atrogriseum Acremonium bacillisporum Acremonium bactrocephalum Acremonium biseptum Acremonium ... blochii Acremonium borodinense Acremonium brachypenium Acremonium breve Acremonium brunnescens Acremonium byssoides Acremonium ... Acremonium curvulum Acremonium cymosum Acremonium dichromosporum Acremonium diospyri Acremonium domschii Acremonium egyptiacum ...
Cases involving different species of Acremonium are often reported as simply as an Acremonium species, which reduces the amount ... Acremonium strictum grows readily at 30 °C on glucose peptone agar, showing mycelium of approximately 50mm in size in 7 days. ... The genus Acremonium is a large polyphyletic genus of approximately 150 species, many of which are derived from a closely ... In Maclura cochinchinensis, Acremonium strictum acts as an endophytic fungi that infects primarily the leaves of the plant. In ...
... Richard Antrobus, Gabriel Wong, Julie ... A. C. Pastorino, U. P. De Menezes, H. H. D. S. Marques et al., "Acremonium kiliense infection in a child with chronic ... H. Boltansky, K. J. Kwon-Chung, A. M. Macher, and J. I. Gallin, "Acremonium strictum-related pulmonary infection in a patient ... J. Guarro, W. Gams, I. Pujol, and J. Gené, "Acremonium species: new emerging fungal opportunists-in vitro antifungal ...
... Richard Antrobus, Gabriel Wong, Julie ... Richard Antrobus, Gabriel Wong, Julie Jones, and Aarnoud Huissoon, "Mycetoma Caused by Acremonium Species in a Patient with ...
Acremonium kiliense in oesophagus stenosis. Mycoses 1991;34:257-60. [ Links ]. 4. Brown N.M., Blundell E.L., Chown S.R., et al ... Acremonium infection in a neutropenic patient. J Infect 1992;25:73-6. [ Links ]. 5. Stiehm E.R. New and Old Immunodeficiencies ... Endocardite em prótese de dura-mater provocada pelo Acremonium kiliense. Rev Inst Med Trop São Paulo 1981;23:274-9. [ Links ]. ... 1. Lopes J.O., Kolling L.C., Neumaier W. Kerionlike lesion of the scalp due to Acremonium kiliense in a noncompromised boy. Rev ...
... of Acremonium stromaticum, a hyaline hyphomycete (glassy, lightly pigmented conidial fungus) that occurs widely in nature but ... Keywords: 211168a, acremonium, allergen, allergenic, allergens, ameroconidia, ameroconidium, animal, arthritis, ascomycota, ... Caption: Coloured scanning electron micrograph (SEM) of Acremonium stromaticum, a hyaline hyphomycete (glassy, lightly ...
Complementation of a C. acremonium cefG mutant, as well as expression of the gene in Aspergillus niger, showed this ORF to be ... A long open reading frame (ORF) closely linked to the Cephalosporium acremonium gene cefEF was identified by DNA sequencing. ... Cloning, characterization, and use in strain improvement of the Cephalosporium acremonium gene cefG encoding acetyl transferase ...
Parasarocladium for the Acremonium radiatum complex and Kiflimonium for the Acremonium curvulum complex. ... The recently designated type species of the genus Acremonium, A. alternatum, is known only from a single isolate, but it is the ... the definitive match of the historical concept of Acremonium: a pale orange or dull greenish-coloured monophialidic hyphomycete ... Acremonium is known to be regularly isolated from food and also to be a cause of human disease. Herein, we resolve some sources ...
Browse by Exposure: Acremonium furcatum (1 article). % of records by year: 1965 2017 ...
Acremonium in Mold Types & Images /by Carolyn Willbanks. A toxigenic mold type that evolves in its appearance over time. It ... Exposure to acremonium is very dangerous and it can lead to disease in the bone marrow, immune system and other organs. Because ... Acremonium mold is often pink, grey, orange or white in color. It typically grows in household systems and areas such as ... Acremonium is sometimes known to grow with other types of mold including strachybotrys, which creates a particularly unpleasant ...
"Acremonium" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... This graph shows the total number of publications written about "Acremonium" by people in this website by year, and whether " ... Below are the most recent publications written about "Acremonium" by people in Profiles. ...
The gene for bifunctional deacetoxycephalosporin C synthetase/hydroxylase of Cephalosporium acremonium was cloned and ... The gene for bifunctional deacetoxycephalosporin C synthetase/hydroxylase of Cephalosporium acremonium was cloned and ... Refolding and Purification of Cephalosporium Acremonium Deacetoxycephalosporin C synthetase/hydroxylase From Granules of ...
Species of the genus Acremonium (Cephalosporium) are opportunistic micro-organisms that are environmentally widespread ... Acremonium / isolation & purification*. Fatal Outcome. Female. Fungemia / microbiology*. Humans. Infant, Newborn. Infant, ... A preterm infant with Acremonium strictum fungaemia is reported here. The patient was born at 27 weeks gestation and weighed ... Species of the genus Acremonium (Cephalosporium) are opportunistic micro-organisms that are environmentally widespread ...
Download Free Full-Text of an article GENETIC MANIPULATION OF CEPHALOSPORIN C PRODUCING ACREMONIUM STRAIN FOR INCREASING ... Acremonium chrysogenum DSM 2399 is used as parent strain. Mutagenesis is performed on solid form of optimized production media ... GENETIC MANIPULATION OF CEPHALOSPORIN C PRODUCING ACREMONIUM STRAIN FOR INCREASING ANTIBIOTIC TITER. ...
Acremonium coenophialum was isolated from excised roots of EI seedlings grown on diverse agar media and from sterile coarse ... Tall fescue is thought to have co-evolved with the fungal endophyte Acremonium coenophialum to form a mutualistic relationship ... Studies of the fungal endophyte Acremonium coenophialum in tall fescue Public Deposited ...
Production of Cephalosporin C by Acremonium chrysogenum Grown on Beet Molasses: Optimization of Process Parameters Through ... Production of cephalosporin C by Acremonium chrysogenum in semisynthetic medium. Process Biochem., 38: 263-272.. Kimmel, S.A., ... The organism used throughout this study is a new Egyptian soil Acremonium chrysogenum isolate deposited in Pasteur Institute ( ... Unraveling the methionine-cephalosporin puzzle in Acremonium chrysogenum. Trends Biotechnol., 20: 502-507.. Direct Link , Mason ...
Isolation of Acremonium species producing cephalosporine C (CPC) from forest soil in Gilan province, Iran * MR Sarookhani ... Keywords: Acremonium persicinum, Soil, Cephalosporin C, Iran. Abstract. Cephalosporin C (CPC) is a major precursor of ... From 350 fungi isolated, 21 (6%) were Acremonium spp, which one strain was able to produce CPC. This species was recognized as ... Soil samples were collected and cultured on selective media and Acremonium sp. were isolated. The presence of CPC antibiotic in ...
Loke, P.,Sim, T.-W. (2000). Mutational analysis of tyrosine-191 in the catalysis of Cephalosporium acremonium isopenicillin N ... Mutational analysis of tyrosine-191 in the catalysis of Cephalosporium acremonium isopenicillin N synthase. ...
A tripeptide R-X-S motif which consists of arginine-281 and serine-283 (Cephalosporium acremonium IPNS numbering) was found to ... Using site-directed mutagenesis, arginine-281 in C. acremonium IPNS (cIPNS) was earlier found to be essential for catalysis by ... Loke, P.,Sim, T.-S. (1998). Mutational evidence for the role of serine-283 in Cephalosporium acremonium isopenicillin N ... Mutational evidence for the role of serine-283 in Cephalosporium acremonium isopenicillin N synthase. ...
Acremonium cellulolyticus C-1 and an ethanol producer Saccharomyces cerevisiae. Furthermore, the operational conditions of the ... Kansarn S: A novel concept for the enzymatic degradation mechanism of native cellulose by Acremonium cellulolyticus. Sch Electr ... Park EY, Naruse K, Kato T: Improvement of cellulase production in cultures of Acremonium cellulolyticus using pretreated waste ... Prasetyo J, Zhu J, Kato T, Park EY: Efficient production of cellulase in the culture of Acremonium cellulolyticus using ...
Your trusted lab partner for Acremonium kiliense IgE testing, Viracor Eurofins delivers your results faster, when it matters ...
Sándor, E., Szentirmai, A., Biró, S., & Karaffa, L. (1999). Specific cephalosporin C production of Acremonium chrysogenum is ... Sándor, E, Szentirmai, A, Biró, S & Karaffa, L 1999, Specific cephalosporin C production of Acremonium chrysogenum is ... keywords = "Acremonium chrysogenum, Cephalosporin C, Dialysis reactor",. author = "Erzs{\e}bet S{\a}ndor and Attila ... Specific cephalosporin C production of Acremonium chrysogenum is independent of the culture density. / Sándor, Erzsébet; ...
Mycocosm Portal version:12.104 jgi-myco-web-2.nersc.gov Release Date:30-Jul-2020 15:17:52 PST Current Date:09-Aug-2020 23:02:43.754 PDT ...
Fungus of the Month: Acremonium species. By Dr. Srivandana Kilambi. Acremonium, a filamentous and delicate deuteromycete (fungi ... 1: Microscopic photo of an Acremonium species.. Microscopic features of Acremonium include fine, hyaline hyphae, long awl ... Acremonium is often found growing with Stachybotrys and, similar to Stachybotrys, the spores of this fungus are produced in a ... Acremonium has a high water affinity (Aw 0.90-0.98) and is often isolated from cooling coils, drain pans, window seals, and ...
Cephal acremonium) IgG4 testing, Viracor Eurofins delivers your results faster, when it matters most. ... 2F76822-acremonium-kiliense-cephal-acremonium-igg4%2F&base_url=https%3A%2F%2Fwebdev.viracor-eurofins.com%2Ftest-menu%2F76822- ... acremonium-kiliense-cephal-acremonium-igg4%2F&user_agent=CCBot%2F2.0+(https%3A%2F%2Fcommoncrawl.org%2Ffaq%2F) ... webdev.viracor-eurofins.com/test-menu/76822-acremonium-kiliense-cephal-acremonium-igg4/ ...
  • Acremonium is a genus of fungi in the family Hypocreaceae. (wikipedia.org)
  • From 350 fungi isolated, 21 (6%) were Acremonium spp, which one strain was able to produce CPC. (ajol.info)
  • A variety of fungi, including Paecilomyces, Acremonium, and Sporothrix species, has been associated with endophthalmitis following intraocular surgery or trauma. (medscape.com)
  • Acremonium kiliense infection in a child with chronic granulomatous disease," Brazilian Journal of Infectious Diseases , vol. 9, no. 6, pp. 529-534, 2005. (hindawi.com)
  • We report on a four-month-old child with pneumonia caused by the fungus Acremonium kiliense as the first clinical manifestation of chronic granulomatous disease. (scielo.br)
  • Acremonium kiliense is a saprophytic fungus, characterized by light microscopy as a Gram-positive microorganism with septate hyphae, which presents slow growth in specific culture (Sabouraud agar, glucose and malt extract). (scielo.br)
  • Other opportunistic agents, such as Nocardia asteroids, Francisella philomiragia, Acremonium kiliense, and Legionella gormanii , have been detected in patients with phagocyte disorders [10- (scielo.br)
  • We describe the clinical manifestations, etiological investigation and the therapeutic approach used for a CGD child with a persistent lung process since the 25th day of life, which was found to be caused by Acremonium kiliense . (scielo.br)
  • Ziehl-Neelsen staining was negative for BAAR and culture in Saboraud agar identified Acremonium kiliense , an opportunistic fungus. (scielo.br)
  • Complementation of a C. acremonium cefG mutant, as well as expression of the gene in Aspergillus niger, showed this ORF to be the cefG gene, encoding cephalosporin C acetyltransferase, which catalyzes the last step in CPC biosynthesis. (nih.gov)
  • Acremonium" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
  • Soil samples were collected and cultured on selective media and Acremonium sp. (ajol.info)
  • Coloured scanning electron micrograph (SEM) of Acremonium stromaticum, a hyaline hyphomycete (glassy, lightly pigmented conidial fungus) that occurs widely in nature but is also encountered as a contaminant. (sciencephoto.com)
  • The aim of this study was to evaluate the effect of feeding Acremonium charticola -fermented cassava pulp (AC-FCP) on the intestinal microbial ecology and hematological indices of broiler chickens. (blogspot.in)
  • Exposure to acremonium is very dangerous and it can lead to disease in the bone marrow, immune system and other organs. (mold-help.org)
  • Acremonium, under certain conditions, can also produce very potent mycotoxins called trichothecenes. (mold-help.org)
  • This graph shows the total number of publications written about "Acremonium" by people in this website by year, and whether "Acremonium" was a major or minor topic of these publications. (umassmed.edu)
  • Using site-directed mutagenesis, arginine-281 in C. acremonium IPNS (cIPNS) was earlier found to be essential for catalysis by our group. (nus.edu.sg)