Acinetobacter calcoaceticus: A species of gram-negative, aerobic bacteria found in soil and water. Although considered to be normally nonpathogenic, this bacterium is a causative agent of nosocomial infections, particularly in debilitated individuals.Acinetobacter: A genus of gram-negative bacteria of the family MORAXELLACEAE, found in soil and water and of uncertain pathogenicity.Acinetobacter Infections: Infections with bacteria of the genus ACINETOBACTER.Acinetobacter baumannii: A species of gram-negative, aerobic bacteria, commonly found in the clinical laboratory, and frequently resistant to common antibiotics.Glucose Dehydrogenases: D-Glucose:1-oxidoreductases. Catalyzes the oxidation of D-glucose to D-glucono-gamma-lactone and reduced acceptor. Any acceptor except molecular oxygen is permitted. Includes EC 1.1.1.47; EC 1.1.1.118; EC 1.1.1.119 and EC 1.1.99.10.PQQ Cofactor: A pyrrolo-quinoline having two adjacent keto-groups at the 4 and 5 positions and three acidic carboxyl groups. It is a coenzyme of some DEHYDROGENASES.Protocatechuate-3,4-Dioxygenase: An enzyme that catalyzes the oxidation of protocatechuate to 3-carboxy-cis-cis-muconate in the presence of molecular oxygen. It contains ferric ion. EC 1.13.11.3.Catechols: A group of 1,2-benzenediols that contain the general formula R-C6H5O2.Mandelic Acids: Analogs or derivatives of mandelic acid (alpha-hydroxybenzeneacetic acid).4-Hydroxybenzoate-3-Monooxygenase: A flavoprotein that catalyzes the synthesis of protocatechuic acid from 4-hydroxybenzoate in the presence of molecular oxygen. EC 1.14.13.2.Adipates: Derivatives of adipic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain a 1,6-carboxy terminated aliphatic structure.Alkanes: The generic name for the group of aliphatic hydrocarbons Cn-H2n+2. They are denoted by the suffix -ane. (Grant & Hackh's Chemical Dictionary, 5th ed)Glucose 1-Dehydrogenase: A glucose dehydrogenase that catalyzes the oxidation of beta-D-glucose to form D-glucono-1,5-lactone, using NAD as well as NADP as a coenzyme.Parabens: Methyl, propyl, butyl, and ethyl esters of p-hydroxybenzoic acid. They have been approved by the FDA as antimicrobial agents for foods and pharmaceuticals. (From Hawley's Condensed Chemical Dictionary, 11th ed, p872)BenzaldehydesCatechol 1,2-Dioxygenase: An enzyme that catalyzes the oxidation of catechol to muconic acid with the use of Fe3+ as a cofactor. This enzyme was formerly characterized as EC 1.13.1.1 and EC 1.99.2.2.Genes, Bacterial: The functional hereditary units of BACTERIA.Benzyl Alcohols: Alcohols derived from the aryl radical (C6H5CH2-) and defined by C6H5CHOH. The concept includes derivatives with any substituents on the benzene ring.Benzaldehyde Dehydrogenase (NADP+)Sorbic Acid: Mold and yeast inhibitor. Used as a fungistatic agent for foods, especially cheeses.Alcohol Oxidoreductases: A subclass of enzymes which includes all dehydrogenases acting on primary and secondary alcohols as well as hemiacetals. They are further classified according to the acceptor which can be NAD+ or NADP+ (subclass 1.1.1), cytochrome (1.1.2), oxygen (1.1.3), quinone (1.1.5), or another acceptor (1.1.99).DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Alcaligenes: A genus of gram-negative, aerobic, motile bacteria that occur in water and soil. Some are common inhabitants of the intestinal tract of vertebrates. These bacteria occasionally cause opportunistic infections in humans.Carbohydrate Dehydrogenases: Reversibly catalyze the oxidation of a hydroxyl group of carbohydrates to form a keto sugar, aldehyde or lactone. Any acceptor except molecular oxygen is permitted. Includes EC 1.1.1.; EC 1.1.2.; and 1.1.99.Acids, Acyclic: Carboxylic acids that have open-chain molecular structures as opposed to ring-shaped structures.Hydroxybenzoates: Benzoate derivatives substituted by one or more hydroxy groups in any position on the benzene ring.Oxygenases: Oxidases that specifically introduce DIOXYGEN-derived oxygen atoms into a variety of organic molecules.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Benzoates: Derivatives of BENZOIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxybenzene structure.Aldehyde Oxidoreductases: Oxidoreductases that are specific for ALDEHYDES.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Benzyl Alcohol: A colorless liquid with a sharp burning taste and slight odor. It is used as a local anesthetic and to reduce pain associated with LIDOCAINE injection. Also, it is used in the manufacture of other benzyl compounds, as a pharmaceutic aid, and in perfumery and flavoring.Microbial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Carboxy-Lyases: Enzymes that catalyze the addition of a carboxyl group to a compound (carboxylases) or the removal of a carboxyl group from a compound (decarboxylases). EC 4.1.1.Phenol: An antiseptic and disinfectant aromatic alcohol.Benzoic Acid: A fungistatic compound that is widely used as a food preservative. It is conjugated to GLYCINE in the liver and excreted as hippuric acid.Tetracyclines: Closely congeneric derivatives of the polycyclic naphthacenecarboxamide. (Gilman et al., Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th ed, p1117)Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Biodegradation, Environmental: Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.Bacterial Proteins: Proteins found in any species of bacterium.Cross Infection: Any infection which a patient contracts in a health-care institution.Chromosomes, Bacterial: Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.Conjugation, Genetic: A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.Carbapenems: A group of beta-lactam antibiotics in which the sulfur atom in the thiazolidine ring of the penicillin molecule is replaced by a carbon atom. THIENAMYCINS are a subgroup of carbapenems which have a sulfur atom as the first constituent of the side chain.Gram-Negative Aerobic Bacteria: A large group of aerobic bacteria which show up as pink (negative) when treated by the gram-staining method. This is because the cell walls of gram-negative bacteria are low in peptidoglycan and thus have low affinity for violet stain and high affinity for the pink dye safranine.Transformation, Bacterial: The heritable modification of the properties of a competent bacterium by naked DNA from another source. The uptake of naked DNA is a naturally occuring phenomenon in some bacteria. It is often used as a GENE TRANSFER TECHNIQUE.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.EsterasesAmino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Pseudomonas: A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.Gram-Negative Bacteria: Bacteria which lose crystal violet stain but are stained pink when treated by Gram's method.Drug Resistance, Multiple, Bacterial: The ability of bacteria to resist or to become tolerant to several structurally and functionally distinct drugs simultaneously. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Drug Resistance, Microbial: The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Polysaccharides, Bacterial: Polysaccharides found in bacteria and in capsules thereof.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.R Factors: A class of plasmids that transfer antibiotic resistance from one bacterium to another by conjugation.Transformation, Genetic: Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Colistin: Cyclic polypeptide antibiotic from Bacillus colistinus. It is composed of Polymyxins E1 and E2 (or Colistins A, B, and C) which act as detergents on cell membranes. Colistin is less toxic than Polymyxin B, but otherwise similar; the methanesulfonate is used orally.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Enterobacteriaceae: A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock.Coenzymes: Small molecules that are required for the catalytic function of ENZYMES. Many VITAMINS are coenzymes.Quinolones: A group of derivatives of naphthyridine carboxylic acid, quinoline carboxylic acid, or NALIDIXIC ACID.Genetic Complementation Test: A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.Isomerases: A class of enzymes that catalyze geometric or structural changes within a molecule to form a single product. The reactions do not involve a net change in the concentrations of compounds other than the substrate and the product.(from Dorland, 28th ed) EC 5.Molecular Weight: The sum of the weight of all the atoms in a molecule.Operon: In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.

Effects of surfactant mixtures, including Corexit 9527, on bacterial oxidation of acetate and alkanes in crude oil. (1/123)

Mixtures of nonionic and anionic surfactants, including Corexit 9527, were tested to determine their effects on bacterial oxidation of acetate and alkanes in crude oil by cells pregrown on these substrates. Corexit 9527 inhibited oxidation of the alkanes in crude oil by Acinetobacter calcoaceticus ATCC 31012, while Span 80, a Corexit 9527 constituent, markedly increased the oil oxidation rate. Another Corexit 9527 constituent, the negatively charged dioctyl sulfosuccinate (AOT), strongly reduced the oxidation rate. The combination of Span 80 and AOT increased the rate, but not as much as Span 80 alone increased it, which tentatively explained the negative effect of Corexit 9527. The results of acetate uptake and oxidation experiments indicated that the nonionic surfactants interacted with the acetate uptake system while the anionic surfactant interacted with the oxidation system of the bacteria. The overall effect of Corexit 9527 on alkane oxidation by A. calcoaceticus ATCC 31012 thus seems to be the sum of the independent effects of the individual surfactants in the surfactant mixture. When Rhodococcus sp. strain 094 was used, the alkane oxidation rate decreased to almost zero in the presence of a mixture of Tergitol 15-S-7 and AOT even though the Tergitol 15-S-7 surfactant increased the alkane oxidation rate and AOT did not affect it. This indicated that there was synergism between the two surfactants rather than an additive effect like that observed for A. calcoaceticus ATCC 31012.  (+info)

Genotypic and phenotypic similarity of multiresistant Acinetobacter baumannii isolates in the Czech Republic. (2/123)

The diversity of 103 clinical isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex obtained between 1991 and 1997 from 17 Czech hospitals was studied by ribotyping, biotyping, plasmid profiling and antibiotic susceptibility testing. According to the EcoRI ribotypes, all but one of these isolates were identified to the DNA group level: 77 isolates were allocated to DNA group 2 (A. baumannii), 14 to DNA group 3, 10 to DNA group 13 sensu Tjernberg and Ursing and one to DNA group 1 (A. calcoaceticus). In total, 50 different EcoRI ribotypes and 10 biotypes were observed. Plasmids were found in 92% of the isolates and a high variability in plasmid profiles was found in isolates of the same DNA group. The combination of typing profiles allowed two predominant groups (termed A and B) to be distinguished among the A. baumannii isolates (37 and eight isolates, respectively) that shared a specific ribotype and were highly similar in other properties. These two groups comprised both sporadic and outbreak isolates and were found in most localities. Group A and B isolates were markedly more resistant to antibiotics than most of the remaining isolates, thus representing 85% of all multiresistant isolates. The features of groups A and B corresponded to those of two epidemic clones identified recently among hospital strains in north-western Europe.  (+info)

Functional evaluation of the genes involved in malonate decarboxylation by Acinetobacter calcoaceticus. (3/123)

The genomic locus containing the potential repressor gene mdcY (inactivated by a putative IS3 element) and the mdcLMACDEGBH genes from Acinetobacter calcoaceticus was cloned and sequenced. In order to evaluate the biochemical function of the protein components, the genes were expressed independently and their activities predicted by database analysis. The mdcA gene product, the alpha subunit, was found to be malonate/acetyl-CoA transferase and the mdcD gene product, the beta subunit, was found to be malonyl-CoA decarboxylase. The mdcE gene product, the gamma subunit, may play a role in subunit interaction to form a stable complex or as a codecarboxylase. The mdcC gene product, the delta subunit, was an acyl-carrier protein, which has a unique CoA-like prosthetic group. Various combinations of malonate decarboxylase subunits allowed us to estimate their contribution to malonyl-CoA decarboxylase activity. The prosthetic group was identified as carboxymethylated 2'-(5"-phosphoribosyl)-3'-dephospho-CoA by mass spectrometry. The mdcH gene product was determined to have malonyl-CoA/dephospho-CoA acyltransferase activity. Using database analysis mdcLM, mdcG, mdcB and mdcI were estimated to be the genes for a malonate transporter, a holo-acyl carrier synthase, protein for the formation of precursor of the prosthetic group and a regulatory protein, respectively. From the data shown above we propose a metabolic pathway for malonate in A. calcoaceticus.  (+info)

Autoantibodies to brain components and antibodies to Acinetobacter calcoaceticus are present in bovine spongiform encephalopathy. (4/123)

Bovine spongiform encephalopathy (BSE) is a neurological disorder, predominantly of British cattle, which belongs to the group of transmissible spongiform encephalopathies together with Creutzfeldt-Jakob disease (CJD), kuru, and scrapie. Autoantibodies to brain neurofilaments have been previously described in patients with CJD and kuru and in sheep affected by scrapie. Spongiform-like changes have also been observed in chronic experimental allergic encephalomyelitis, at least in rabbits and guinea pigs, and in these conditions autoantibodies to myelin occur. We report here that animals with BSE have elevated levels of immunoglobulin A autoantibodies to brain components, i.e., neurofilaments (P < 0.001) and myelin (P < 0.001), as well as to Acinetobacter calcoaceticus (P < 0.001), saprophytic microbes found in soil which have sequences cross-reacting with bovine neurofilaments and myelin, but there were no antibody elevations against Agrobacterium tumefaciens or Escherichia coli. The relevance of such mucosal autoantibodies or antibacterial antibodies to the pathology of BSE and its possible link to prions requires further evaluation.  (+info)

3,4-Dihydrocoumarin hydrolase with haloperoxidase activity from Acinetobacter calcoaceticus F46. (5/123)

A novel lactonohydrolase, an enzyme that catalyzes the hydrolysis of 3,4-dihydrocoumarin, was purified 375-fold to apparent homogeneity, with a 22.7% overall recovery, from Acinetobacter calcoaceticus F46, which was isolated as a fluorene-assimilating micro-organism. The molecular mass of the native enzyme, as estimated by high-performance gel-permeation chromatography, is 56 kDa, and the subunit molecular mass is 30 kDa. The enzyme specifically hydrolyzes 3,4-dihydrocoumarin, and the Km and Vmax for 3,4-dihydrocoumarin are 0.806 mM and 4760 U.mg-1, respectively. The N-terminal and internal amino acid sequences of the enzyme show high similarity to those of bacterial non-heme haloperoxidases. The enzyme exhibits brominating activity with monochlorodimedon in the presence of H2O2 and 3, 4-dihydrocoumarin or an organic acid, such as acetate and n-butyrate.  (+info)

ComP, a pilin-like protein essential for natural competence in Acinetobacter sp. Strain BD413: regulation, modification, and cellular localization. (6/123)

We recently identified a pilin-like competence factor, ComP, which is essential for natural transformation of the gram-negative soil bacterium Acinetobacter sp. strain BD413. Here we demonstrate that transcription and synthesis of the pilin-like competence factor ComP are maximal in the late stationary growth phase, whereas competence is induced immediately after inoculation of a stationary-phase culture into fresh medium. Western blot analyses revealed three forms of ComP, one with an apparent molecular mass of 15 kDa, which correlates with the molecular mass deduced from the DNA sequence, one 20-kDa form, which was found to be glycosylated, and one 23-kDa form. The glycosylation of ComP was not required for its function in DNA binding and uptake. The 20-kDa form was present in the cytoplasmic membrane, the periplasm, and the outer membrane, whereas the 23-kDa form was located in the outer membrane and might be due to a further modification. Immunological data suggest that ComP is not a subunit of the pilus structures. Possible functions of ComP in the DNA transformation machinery of Acinetobacter sp. strain BD413 are discussed.  (+info)

The malonate decarboxylase operon of Acinetobacter calcoaceticus KCCM 40902 is regulated by malonate and the transcriptional repressor MdcY. (7/123)

A regulatory gene-like open reading frame oriented oppositely to mdcL, coined mdcY, was found upstream from the structural genes of the mdcLMACDEGBH operon in Acinetobacter calcoaceticus KCCM 40902. To elucidate the function of this gene, mdcY was expressed in Escherichia coli, and the MdcY protein was purified to homogeneity. Its DNA binding activity and binding site were examined by gel retardation and footprinting assays in vitro and by site-directed mutagenesis of the binding sites in vivo. The regulator bound target DNA regardless of the presence of malonate, and the binding site was found centered at -65 relative to the mdcL transcriptional start site and contains a 12-bp palindromic structure (5'-ATTGTA/TACAAT-3'). Using a promoter fusion to the reporter gene luc, we found that the promoter P(mdcY) is negatively regulated by MdcY independent of malonate. However, the promoter P(mdcL) recovered its activity in the presence of malonate. When mdcY was introduced into A. calcoaceticus KCCM 40902 in which the gene is inactivated by an IS3 family element, malonate decarboxylase was significantly repressed in cultures growing in acetate, succinate, or Luria-Bertani medium. However, in cells growing in malonate, malonate decarboxylase was induced, indicating that MdcY is a transcriptional repressor and that malonate or a product resulting from malonate metabolism should be the intracellular inducer of the mdc operon.  (+info)

Ca(2+) stabilizes the semiquinone radical of pyrroloquinoline quinone. (8/123)

Spectroelectrochemical studies were performed on the interaction between Ca(2+) and pyrroloquinoline quinone (PQQ) in soluble glucose dehydrogenase (sGDH) and in the free state by applying a mediated continuous-flow column electrolytic spectroelectrochemical technique. The enzyme forms used were holo-sGDH (the holo-form of sGDH from Acinetobacter calcoaceticus) and an incompletely reconstituted form of this, holo-X, in which the PQQ-activating Ca(2+) is lacking. The spectroelectrochemical and ESR data clearly demonstrated the generation of the semiquinone radical of PQQ in holo-sGDH and in the free state in the presence of Ca(2+). In contrast, in the absence of Ca(2+) no semiquinone was observed, either for PQQ in the free state (at pH 7.0) or in the enzyme (holo-X). Incorporation of Ca(2+) into the active site of holo-X, yielding holo-sGDH, caused not only stabilization of the semiquinone form of PQQ but also a negative shift (of 26.5 mV) of the two-electron redox potential, indicating that the effect of Ca(2+) is stronger on the oxidized than on the reduced PQQ. Combining these data with the observations on the kinetic and chemical mechanisms, it was concluded that the strong stimulating effect of Ca(2+) on the activity of sGDH can be attributed to facilitation of certain kinetic steps, and not to improvement of the thermodynamics of substrate oxidation. The consequences of this conclusion are discussed for the oxidative as well as for the reductive part of the reaction of sGDH.  (+info)

The antibiotic susceptibility of Acinetobacter calcoaceticus-Acinetobacter baumannii complex strains recovered from the intensive care unit (ICU) of West China Hospital, Sichuan, PR China, from 2006 to 2009 was investigated. The identification of A. baumannii and analysis of carbapenemase-encoding genes and their relationship with ISAba1 were performed by PCR. Furthermore, a DiversiLab repetitive extragenic palindromic sequence-based PCR (rep-PCR) microbial typing system and a multilocus sequence typing (MLST) scheme were applied to assess the genetic relationship of the isolates. The results showed that the antibiotic susceptibility of the A. calcoaceticus-A. baumannii complex isolates changed and imipenem resistance increased rapidly between 2006 and 2009. The blaOXA-51-like and ISAba1-associated blaOXA-23 genes were prevalent in the imipenem-resistant A. baumannii isolates. However, the blaOXA-58-like gene was found in only one isolate and no metallo-β-lactamase genes were detected. The ...
Acinetobacter calcoaceticus is a bacterial species of the genus Acinetobacter. It is a nonmotile, gram negative coccobacillus. It grows under aerobic conditions, is catalase positive and oxidase negative. It is part of the normal human intestinal flora. Together with A. baumannii, it is referred to as the A. calcoaceticus-A. baumannii complex, which is relatively simple to identify based on the beforementioned phenotypic characteristics. To identify other Acinetobacter species genotyping is required. A. calcoaceticus is a soil bacterium. It has been shown to be prevalent in the tiger mosquito Aedes albopictus microflora. Phloroglucinol carboxylic acid is a degradation product excreted by A. calcoaceticus grown on (+)-catechin as the sole source of carbon. A. calcoaceticus can be pathogenic and cause an opportunistic infection in patients with multiple underlying diseases. A. calcoaceticus can be used as an alternative to A. baumannii in the laboratory setting. The interchangeability of the two ...
Acinetobacter calcoaceticus BD413 accumulates wax esters and triacylglycerol under conditions of mineral nutrient limitation. Nitrosoguanidine-induced mutants of strain BD413 were isolated that failed to accumulate wax esters under nitrogen-limited growth conditions. One of the mutants, Wow15 (without wax), accumulated wax when grown in the presence of cis-11-hexadecenal and hexadecanol but not hexadecane or hexadecanoic acid. This suggested that the mutation may have inactivated a gene encoding either an acyl-acyl carrier protein or acyl-coenzyme A (CoA) reductase. The Wow15 mutant was complemented with a cosmid genomic library prepared from wild-type A. calcoaceticus BD413. The complementary region was localized to a single gene (acr1) encoding a protein of 32,468 Da that is 44% identical over a region of 264 amino acids to a product of unknown function encoded by an open reading frame associated with mycolic acid synthesis in Mycobacterium tuberculosis H37Ra. Extracts of Escherichia coli ...
Acinetobacter calcoaceticus MopR protein: Member of the NtrC family of transcriptional activators with significant homology to XylR and DmpR from Pseudomonas; regulates phenol degradation in Acinetobacter calcoaceticus; has ATP-binding activity; binds phenol; GenBank CAA93242
Ochratoxin A (OTA) is a mycotoxin with a main nephrotoxic activity contaminating several foodstuffs. In the present report, five soil samples collected from OTA-contaminated vineyards were screened to isolate microorganisms able to biodegrade OTA. When cultivated in OTA-supplemented medium, OTA was converted in OTα by 225 bacterial isolates. To reveal clonal relationships between isolates, molecular typing by using an automated rep-PCR system was carried out, thus showing the presence of 27 different strains (rep-PCR profiles). The 16S-rRNA gene sequence analysis of an isolate representative of each rep-PCR profiles indicated that they belonged to five bacterial genera, namely Pseudomonas, Leclercia, Pantoea, Enterobacter, and Acinetobacter. However, further evaluation of OTA-degrading activity by the 27 strains revealed that only Acinetobacter calcoaceticus strain 396.1 and Acinetobacter sp. strain neg1, consistently conserved the above property; their further characterization showed that they were
Genus and Species: Acinetobacter calcoaceticus (strepomycin sensitive) Domain: Prokaryote Optimal Growth Medium: Brain Heart Infusion Agar Optimal Growth Temperature: 30° C Package: MicroKwik Culture® Vial Biosafety Level: 2 Gram Stain: Gram-Negative Shape: Bacillus (rod-shaped)
Domain architecture and assignment details (superfamily, family, region, evalue) for HMPREF0012_00695T0 from Acinetobacter calcoaceticus ruh2202. Plus protein sequence and external database links.
COVID-19 (short for "Coronavirus Disease 2019) is the disease caused by the novel coronavirus, SARS-CoV-2. Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. COVID-19 represents a particular challenge to people with serious chronic medical conditions such as diabetes. To keep our community as informed as possible, the American Diabetes Association is partnering with the Chan Zuckerberg Initiatives Meta to highlight the latest research developments at the intersection of COVID-19 and diabetes. ...
Summary Different PCR-based DNA fingerprinting techniques were evaluated for typing 26 clinical isolates belonging to the Acinetobacter calcoaceticus-A. baumannii complex. Seven isolates belonged to a previously defined outbreak while 19 isolates were unrelated epidemiologically. The PCR-based DNA fingerprinting techniques used were: (i) repetitive extragenic palindromic (REP) PCR; (ii) enterobacterial repetitive intergenic consensus (ERIC) PCR; (iii) randomly amplified polymorphic DNA with M13 forward primer; (iv) restriction analysis of the amplified 16S rRNA gene (ARDRA-16S); and (v) restriction analysis of an amplified region containing the 16S-23S rRNA spacer region and part of the 23S rRNA gene (ARDRA 23S + spacer). The discrimination index for the PCR-based DNA fingerprinting techniques was: 0.99 for REP; 0.94 for ERIC; 0.87 for M13; 0.60 for ARDRA-16S digested with Hpa II and |0.50 for ARDRA 23S + spacer. It was concluded that REP-PCR possessed high discriminatory power and reproducibility in
TY - JOUR. T1 - Comparative genomic analysis of Acinetobacter oleivorans DR1 To determine strain-specific genomic regions and gentisate biodegradation. AU - Jung, Jaejoon. AU - Madsen, Eugene L.. AU - Jeon, Che Ok. AU - Park, Woojun. PY - 2011/10. Y1 - 2011/10. N2 - The comparative genomics of Acinetobacter oleivorans DR1 assayed with A. baylyi ADP1, A. calcoaceticus PHEA-2, and A. baumannii ATCC 17978 revealed that the incorporation of phage-related genomic regions and the absence of transposable elements have contributed to the large size (4.15 Mb) of the DR1 genome. A horizontally transferred genomic region and a higher proportion of transcriptional regulator- and signal peptide-coding genes were identified as characteristics of the DR1 genome. Incomplete glucose metabolism, metabolic pathways of aromatic compounds, biofilm formation, antibiotics and metal resistance, and natural competence genes were conserved in four compared genomes. Interestingly, only strain DR1 possesses gentisate ...
Lingelsheimia anitrata --, acinetobacter calcoaceticus a species of gram-negative, aerobic bacteria found in soil and water. Although considered to be normally nonpathogenic, this bacterium is a causative agent of nosocomial infections, particularly in debilitated individuals. ...
Accurate and robust monitoring of product and reactants in a complex bioconversion stream is essential for the development of effective process control strategies. To monitor a microbially-catalysed Baeyer-Villiger bioconversion of a cyclic ketone to an optically pure lactone, a near infrared (NIR) spectroscopic method has been developed. The reaction, catalysed by cyclohexanone monooxygenase from Acinetobacter calcoaceticus (expressed in Escherichia coli) is characterised by substrate (ketone) and product (lactone) inhibition at relatively low concentrations. Quantitative multivariate calibration of a NIR spectrophotometer for ketone and lactone resulted in a standard error of prediction (SEP) at-line of 0.088 and 0.110 g/l-1 and on-line of 0.130 and 0.180 g/l-1 , respectively. The directed modification of quantitative models, by the inclusion of spiked process samples improved the SEP for lactone prediction where bioprocess development meant existing NIR models were not relevant. The ...
Quinoprotein glucose dehydrogenase (EC 1.1.99.17) from Acinetobacter calcoaceticus L.M.D. 79.41 was purified to homogeneity. It is a basic protein with an isoelectric point of 9.5 and an Mr of 94,000. Denaturation yields two molecules of PQQ/molecule and a protein with an Mr of 48000, indicating that the enzyme consists of two subunits, which are probably identical because even numbers of aromatic amino acids were found. The oxidized enzyme form has an absorption maximum at 350 nm, and the reduced form, obtained after the addition of glucose, at 338 nm. Since double-reciprocal plots of initial reaction rates with various concentrations of glucose or electron acceptor show parallel lines, and substrate inhibition is observed for glucose as well as for electron acceptor at high concentrations, a ping-pong kinetic behaviour with the two reactants exists. From the plots, Km values for glucose and Wursters Blue of 22 mM and 0.78 mM respectively, and a Vmax. of 7.730 mumol of glucose oxidized/min per ...
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The demand for new methods through which important enantiopure compounds can be produced is increasing within the chemical industry. In this regard, one group of interesting compounds are enantiopure carboxylic acids as traditional methods towards producing these compounds that rely on a process known as enantioselective decarboxylative protonation (EDP) are often insufficient due to a combination of factors including low yields, low enantioselectivities, the need for environmentally deleterious reaction conditions and high costs. Due to the shortcomings of these synthetic techniques, the application of biological systems is an alternative method that has come to the forefront of current research, which is because biological systems often boast a number of advantages over traditional chemical methods. Described within this thesis is one such biological method towards the production of enantiopure carboxylic acid compounds via EDP, a method that utilises the enzyme aryl malonate decarboxylase ...
ID ACIBC_1_PE11 STANDARD; PRT; 305 AA. AC ACIBC_1_PE11; B2I3S8; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Transposase; (ACIBC_1.PE11). GN Name=insB; OrderedLocusNames=ACICU_p0013; OS ACINETOBACTER BAUMANNII ACICU. OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii OC complex. OX NCBI_TaxID=405416; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS ACIBC_1.PE11. CC Acinetobacter baumannii ACICU plasmid pACICU1, complete sequence. CC sequence. CC -!- ANNOTATIONS ORIGIN:B2I3S8_ACIBC CC -!- GENE_FAMILY: HOG000023134 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; B2I3S8; -. DR EMBL; CP000864; ACC58990.1; -; Genomic_DNA. DR RefSeq; YP_001840868.1; NC_010605.1. DR ProteinModelPortal; B2I3S8; -. DR STRING; B2I3S8; -. DR GeneID; 6233706; -. DR GenomeReviews; CP000864_GR; insB. DR ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii ...
endodeoxyribonuclease AclI: type II restriction enzyme, EC 3.1.21.4, from Acinetobacter calcoaceticus; recognizes 5-AA/CGTT-3 and cleaves at the slash
Malonate decarboxylase, like citrate lyase, has a unique acyl carrier protein subunit with a prosthetic group derived from, and distinct from, coenzyme A. Members of this protein family are the phosphoribosyl-dephospho-CoA transferase specific to the malonate decarboxylase system. This enzyme can also be designated holo-ACP synthase (2.7.7.61). The corresponding component of the citrate lyase system, CitX, shows little or no sequence similarity to this family ...
Five methods for assaying bacterial surface hydrophobicity, namely, bacterial adherence to hydrocarbons, salt aggregation, hydrophobic interaction chromatography, adhesion to polystyrene and latex particle agglutination were used to compare the hydrophobic surface properties of Escherichia coli, Acinetobacter calcoaceticus, Staphylococcus aureus and Streptococcus mitis. Two strains of A. calcoaceticus, including RAG-1, gave strong positive results by all five methods. S. mitis gave weak or negative results by all methods. The results for the other bacteria varied with the method. We conclude that reliance on one method for such tests is inadequate ...
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... As definições de siglas Acinetobacter. As definições de acrónimo Acinetobacter. Sigla Acinetobacter significa para. Além de encontrar siglas. Encontre o que significam as siglas!
海词词典,最权威的学习词典,专业出版acinetobacter baummanii是什么意思,acinetobacter baummanii的用法,acinetobacter baummanii翻译和读音等详细讲解。海词词典:学习变容易,记忆很深刻。
海词词典,最权威的学习词典,专业出版acinetobacter winogradskyii是什么意思,acinetobacter winogradskyii的用法,acinetobacter winogradskyii翻译和读音等详细讲解。海词词典:学习变容易,记忆很深刻。
Ive been told that I have an Acinetobacter Baumanni infection at a surgery site. Ive also been told not to worry about it and that the Augmentin I finished 10 days ago would clear it up ...
Las especies de Acinetobacter son, relativamente, microorganismos inofensivos con una gran capacidad de persistir en los ambientes hospitalarios por periodos prolongados como colonizantes y pudiendo ser una causa importante ...
The five enzymes in the catechol degradation pathway not naturally present in E. coli will be introduced into E. coli as BioBrick parts. The parts will then be used to construct five recombinant strains of E. coli (Figure 2). These include a full reconstruction of the pathway (control strain) and several partial reconstructions (test strains). The latter will contain pathway holes corresponding to all combinations of sequential pairs of enzymes, with complementary fusion pairs. The test strain with the fusion of enzymes 3 and 4 will be of prime interest, while the other test strains will be used validate the predictions made by Cell++. Many of the enzymes in the system are multimeric. For this reason we propose expressing the fusion proteins alongside their native (un-fused) counterparts to promote linkage of the enzymes while allowing for assembly of unlinked monomers onto the fusions which we refer to as seeds. The relative expression of free monomers and fusion seeds is predicted to be ...
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The study of biofilm function, structure and microbial interactions might help to improve our understanding of biofilm wastewater treatment processes. However, few reports specifically address the influence of interactions within multispecies biofilms on microbial activity and biofilm composition. Thus, the relationship between biofilm formation, denitrification activity, phosphorus removal and the composition of extracellular polymeric substances (EPS), exopolysaccharides and the bacterial community was investigated using biofilms of denitrifying and phosphorus removing strains Comamonas denitrificans 110, Brachymonas denitrificans B79, Aeromonas hydrophila L6 and Acinetobacter calcoaceticus ATCC23055. Denitrification activity within the biofilms generally increased with the amount of biofilm while phosphorus removal depended on bacterial growth rate. Synergistic effects of co-growth on denitrification (B. denitrificans B79 and A. hydrophila L6) and phosphorus removal (C. denitrificans 110 with ...
Acinetobacter baumannii is nowadays a relevant nosocomial pathogen characterized by multidrug resistance (MDR) and concomitant difficulties to treat infections. OmpA is the most abundant A. baumannii outer membrane (OM) protein, and is involved in virulence, host-cell recognition, biofilm formation, regulation of OM stability, permeability and antibiotic resistance. OmpA members are two‐domain proteins with an N‐terminal eight‐stranded β‐barrel domain with four external loops (ELs) interacting with the environment, and a C‐terminal periplasmic domain binding non‐covalently to the peptidoglycan. Here, we combined data from genome sequencing, phylogenetic and multilocus sequence analyses from 975 strains/isolates of the Acinetobacter calcoaceticus/Acinetobacter baumannii complex (ACB), 946 from A. baumannii, to explore ompA microevolutionary divergence. Five major ompA variant groups were identified (V1 to V5) in A. baumannii, encompassing 52 different alleles coding for 23 different ...
Ver más] As a part of a nationwide study in Spain, 15 clinical isolates of Acinetobacter genomic species 3 (AG3) were analyzed. The main objective of the study was to characterize the ampC genes from these isolates and to determine their involvement in B-lactam resistance in AG3. The 15 AG3 isolates showed different profiles of resistance to ampicillin (range of MICs, 12 to ,256 μg/ml). Nucleotide sequencing of the 15 ampC genes yielded 12 new AmpC enzymes (ADC-12 to ADC-23). The 12 AG3 enzymes showed 93.7 to 96.1% amino acid identity with respect to the AmpC enzyme from Acinetobacter baumannii (ADC-1 enzyme). Eight out of fifteen ampC genes were expressed in Escherichia coli cells under the control of a common promoter, and with the exception of one isolate (isolate 65, which showed lower B-lactam MICs), significant differences in overall B-lactam MICs for E. coli cells expressing AG3 ampC genes were not revealed. No significant differences in ampC gene expression in AG3 clinical isolates ...
Loffhagen, N.; Haertig, C.; Benndorf, D.; Babel, W., 2002: Effects of growth temperature and lipophilic carbon sources on the fatty acid composition and membrane lipid fluidity of Acinetobacter calcoaceticus 69V
AM-1155 is a new quinolone with a wide spectrum of antibacterial activity against various bacteria including anaerobes and Mycoplasma pneumoniae. AM-1155 was 2- to 16-fold more active than ciprofloxacin and ofloxacin against Staphylococcus aureus including methicillin-resistant strains, Staphylococcus epidermidis, Streptococcus pneumoniae, and Enterococcus faecalis; its MICs for 90% of strains tested were 0.10 to 0.78 micrograms/ml. The activity of AM-1155 was comparable to that of ciprofloxacin against members of the family Enterobacteriaceae, Branhamella catarrhalis, Haemophilus influenzae, and Neisseria gonorrhoeae, but was fourfold less than that of ciprofloxacin against Pseudomonas aeruginosa. Against Xanthomonas maltophilia, Acinetobacter calcoaceticus, and Campylobacter jejuni, AM-1155 was two- to fourfold more active than ciprofloxacin. At a concentration of 1.56 micrograms/ml, AM-1155 inhibited 90% of Bacteroides fragilis strains tested; its activity was 8- to 10-fold higher than those ...
臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。. To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of "NTU Repository" with "Academic Hub" to form NTU Scholars.. ...
The gut microbiota regulates T cell functions throughout the body. We hypothesized that intestinal bacteria impact the pathogenesis of multiple sclerosis (MS), an autoimmune disorder of the CNS and thus analyzed the microbiomes of 71 MS patients not undergoing treatment and 71 healthy controls. Although no major shifts in microbial community structure were found, we identified specific bacterial taxa that were significantly associated with MS. Akkermansia muciniphila and Acinetobacter calcoaceticus, both increased in MS patients, induced proinflammatory responses in human peripheral blood mononuclear cells and in monocolonized mice. In contrast, Parabacteroides distasonis, which was reduced in MS patients, stimulated antiinflammatory IL-10-expressing human CD4+CD25+ T cells and IL-10+FoxP3+ Tregs in mice. Finally, microbiota transplants from MS patients into germ-free mice resulted in more severe symptoms of experimental autoimmune encephalomyelitis and reduced proportions of IL-10+ Tregs ...
การจำแนกเชื้อ Acinetobacter species สามารถใช้วิธี multiplex PCR เพื่อตรวจหา natural occurring blaOXA gene ที่จำเพาะต่อเชื้อแต่ละ species ได้ ดังนี้ blaOXA23 ในเชื้อ blaOXA134 ในเชื้อ A. lwoffii/A. schindleri, blaOXA211 ในเชื้อ A. johnsonii, blaOXA213 ในเชื้อ A. calcoaceticus, blaOXA214 ในเชื้อ A. haemolyticus, และ blaOXA228 ในเชื้อ A. bereziniae. ...
In order to determine which enzymes would most benefit from metabolic channeling in the catechol degradation pathway, we used a cellular simulation tool, developed in our lab, called Cell++. It allows us to place enzymes of choice in a compartment within a cellular environment and calculates the effect of localization on metabolite concentrations in a user-defined biochemical pathway (Sanford et al. 2006). We gathered kinetic data of the five enzymes in the degradation pathway and investigated the effects of localizing pairs of sequential enzymes (i.e. catechol 1, 2-dioxygenase and muconate cycloisomeras) on metabolite concentrations. Four co-localization simulations were performed in Cell++ along with a control simulation where no enzymes were co-localized. The initial metabolite in the simulation environment, catechol, was added into the simulation environment and the simulation environment was run for 10,000 iterations to observe the effect on catechol degradation in each simulation. The ...
Acinetobacter baumannii is a strain of bacteria in the Acinetobacter genus. This genome was published to the ATCC Genome Portal on 2020-08-03
The World Health Organization has just released its list of the 12 bacteria that pose the greatest risk to humanitys existence, stating that new antibiotics are "urgently needed" to counter them. WHO held a press conference recently to unveil the list of the most dangerous "superbugs," which are resistant to most antibiotics making them difficult or nearly impossible to treat.. The bacteria Acinetobacter baumannii topped the list. This disease can result in pneumonia, blood infections, and more. It affects people with compromised immune systems, and it attacks organ systems with a high fluid content, like the respiratory or urinary tract. ...
Acinetobacter sp. ATCC ® 49467D™ Designation: Genomic DNA from Acinetobacter sp. strain AmMS 248 TypeStrain=False Application:
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Acinetobacter bouvetii.
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The Muck Grit was designed for demanding farm-ranch and construction use. A protective rubber to the exterior gives puncture resistance while remaining flexible with every step. The interior utilizes 5mm of CR Flex-Foam lining layered with PK mesh to improve air circulation. The all-new rubber cup outsole will give superior shock absorption with ex
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Optimum conditions for the activity of the new DNA methylase in cell lysate were determined. Methylation of DNAs of bacteriophages λ and T7 and plasmid pBR322 (dcm+) in the 5′-Cm5CWGG-3′ region blocked M.AjnI activity. The specificity of M.AjnI was determined using λ DNA methylated by this enzyme as well as computer modeling and data on the sensitivity of restriction endonucleases Mval, HinfI, and BstMAI to methylation.
Scientists have recently identified a resistance protein that allows acinetobacter baumannii bacteria to survive chlorhexidine, an antiseptic commonly used in wipes, cleansers and mouthwashes in hospitals. The resistance protein has been called Acinetobacter Chlorhexidine Efflux, abbreviated to Ace.
Complementing our current clinical pipeline, we have three additional drug candidates in preclinical development. AR-501 has broad bactericidal activity against Gram-negative and Gram-positive bacteria, including antibiotic-resistant strains. AR-201 is a human IgG1 mAb directed against the F-protein of respiratory syncytial virus (RSV). AR-401 is a mAb discovery program to treat infections caused by the Gram-negative bacterium Acinetobacter baumannii.. ...
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Duine JA, Frank J, van Zeeland JK (1979). "Glucose dehydrogenase from Acinetobacter calcoaceticus: a 'quinoprotein'". FEBS Lett ...
Geiger, O.; Gorisch, H. (1986). "Crystalline quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus". Biochemistry ... "Cloning of the gene encoding quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus: evidence for the presence of ... "Soluble and membrane-bound quinoprotein D-glucose dehydrogenases of the Acinetobacter calcoaceticus : the binding process of ... "Quinoprotein D-glucose dehydrogenase of the Acinetobacter calcoaceticus respiratory chain: membrane-bound and soluble forms are ...
... a new restriction endonuclease from Acinetobacter calcoaceticus". Nucleic Acids Res. 13 (24): 8685-94. doi:10.1093/nar/13.24. ... a new restriction endonuclease from Acinetobacter calcoaceticus recognizing 5'-AA^CGTT-3'". Nucleic Acids Res. 20 (14): 3787. ... a novel restriction endonuclease from Acinetobacter calcoaceticus BS (Article in russian)". Prikl Biokhim Mikrobiol. 33 (5): ... Morgan RD, Dalton M, Stote R (September 1987). "A unique type II restriction endonuclease from Acinetobacter lwoffi N". Nucleic ...
Among bacteria, degradation of (+)-catechin can be achieved by Acinetobacter calcoaceticus. Catechin is metabolized to ... catechin by Acinetobacter calcoaceticus MTC 127". Biochimica et Biophysica Acta (BBA) - General Subjects. 1621 (3): 261-265. ...
Acinetobacter calcoaceticus strain AIMST Nalbe4 16S ribosomal RNA gene, partial sequence. NCBI. Acinetobacter calcoaceticus ... Acinetobacter calcoaceticus strain AIMST Ngme3 16S ribosomal RNA gene, partial sequence. NCBI. Acinetobacter calcoaceticus ... Moraxellaceae Acinetobacter Acinetobacter calcoaceticus strain AIMST Nalbe4 (N. alba; Mount Jerai, Kedah, Malaysia; stem tissue ... Acinetobacter calcoaceticus strain AIMST Nalbe10 (N. alba; Mount Jerai, Kedah, Malaysia; leaf tissue) Acinetobacter ...
2-dioxygenase from Acinetobacter calcoaceticus: purification and properties". J. Bacteriol. 127 (1): 536-544. PMC 233087 . PMID ... Acinetobacter calcoaceticus, Trichosporon cutaneum, Rhodococcus erythropolis, Frateuria sp., Rhizobium trifolii, Pseudomonas ...
In particular, it has been shown that the Acinetobacter calcoaceticus ADP1 strain synthesizes wax esters through a bifunctional ... distribution and function of wax esters in Acinetobacter calcoaceticus". J. Gen. Microbiol. 132: 3147-3157. doi:10.1099/ ... diacylglycerol acyltransferase mediates wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus ADP1". J. ... Finally, Acinetobacter has been considered as an alternative source for jojoba-like wax ester production, but is limited by the ...
"Functional evaluation of the genes involved in malonate decarboxylation by Acinetobacter calcoaceticus". Eur. J. Biochem. 266: ...
"Functional evaluation of the genes involved in malonate decarboxylation by Acinetobacter calcoaceticus". Eur. J. Biochem. 266: ...
4-diaminobutyrate decarboxylase from Acinetobacter calcoaceticus". J. Gen. Microbiol. 138 (7): 1461-5. doi:10.1099/00221287-138 ... 4-diaminobutyrate decarboxylase of Acinetobacter baumannii". FEMS Microbiol. Lett. 124 (2): 225-8. doi:10.1111/j.1574-6968.1994 ... 3-diaminopropane production pathway in Acinetobacter baumannii". J. Bacteriol. 179 (16): 5118-25. PMC 179370 . PMID 9260954. ...
Acinetobacter calcoaceticus) galactoglucopolysaccharides (Achromobacter spp., Agrobacterium radiobacter, Pseudomonas marginalis ...
"Autoantibodies to brain components and antibodies to Acinetobacter calcoaceticus are present in bovine spongiform ... Other theories state that the agent is a virus, virino, Spiroplasma species, or Acinetobacter species. The pathogenesis of BSE ...
"Autoantibodies to brain components and antibodies to Acinetobacter calcoaceticus are present in bovine spongiform ... Acinetobacter is a bacterium which some think is the cause of the TSEs. The word prion, coined in 1982 by Stanley B. Prusiner, ... "Acinetobacter phage genome is similar to Sphinx 2.36, the circular DNA copurified with TSE infected particles". Scientific ...
Leainafulvene has weak antibacterial activity against Acinetobacter calcoaceticus, and has pronounced cytotoxic activity ...
... the benzoate permeases of Acinetobacter calcoaceticus and E. coli. These proteins are about 400 residues in length and probably ... The generalized transport reaction catalyzed by BenE of A. calcoaceticus is: Benzoate (out) + H+ (out) → Benzoate (in) + H+ (in ...
Acinetobacter anitratus, Acinetobacter calcoaceticus, Actinomyces odontolyticus, Aeromonas hydrophila, Bacteroides distasonis, ... some Acinetobacter spp., Bacteroides fragilis, and Enterococcus faecalis have developed resistance to imipenem to varying ... Bacteroides uniformis, and Clostridium perfringens are generally susceptible to imipenem, while Acinetobacter baumannii, ...
... "cis-diol dehydrogenases encoded by the TOL pWW0 plasmid xylL gene and the Acinetobacter calcoaceticus chromosomal benD gene are ...
... "cis-diol dehydrogenases encoded by the TOL pWW0 plasmid xylL gene and the Acinetobacter calcoaceticus chromosomal benD gene are ...
1 integron genes in multiple-antimicrobial-resistant clinical isolates of Acinetobacter baumannii-Acinetobacter calcoaceticus ...
A few of the popular examples of microbial biosurfactants includes Emulsan produced by Acinetobacter calcoaceticus, ...
Oscillatoria borneti Pleurococcus Protococcus Scenedesmus quadricauda Selenastrum gracile Acinetobacter calcoaceticus Aeromonas ...
... and Acinetobacter calcoaceticus. Examples of skin conditions treated with ampicillin-sulbactam are moderate to severe diabetic ... It is also capable of binding to the PBP of Bacteroides fragilis and Acinetobacter spp., even when it is given alone. The ... activity of sulbactam against Acinetobacter spp. seen in in-vitro studies makes it distinctive compared to other β-lactamase ...
It is a catechin degradation product excreted by the bacterium Acinetobacter calcoaceticus, a species of bacteria part of the ... catechin by Acinetobacter calcoaceticus MTC 127, Biochimica et Biophysica Acta (BBA), Volume 1621, Issue 3, 11 June 2003, pages ...
Acinetobacter baumannii MeSH B03.440.400.425.537.050.200 --- Acinetobacter calcoaceticus MeSH B03.440.400.425.537.525 --- ... Acinetobacter baumannii MeSH B03.660.250.530.050.200 --- Acinetobacter calcoaceticus MeSH B03.660.250.530.525 --- Moraxella ...
Acinetobacter calcoaceticus Burkholderia cepacia Pseudomonas pseudoalcaligenes Peptostreptococcus spp Fusobacterium necrophorum ... Eubacterium spp Microbacterium spp Acinetobacter spp Bacillus spp Candida albicans Corynebacterium spp Corynebacterium parvum ...
emulsan (Acinetobacter calcoaceticus). *galactoglucopolysaccharides (Achromobacter spp., Agrobacterium radiobacter, Pseudomonas ...
Acinetobacter calcoaceticus on www.ncbi.nlm.nih.gov Type strain of Acinetobacter calcoaceticus at BacDive - the Bacterial ... Acinetobacter calcoaceticus is a bacterial species of the genus Acinetobacter. It is a nonmotile, gram negative coccobacillus. ... To identify other Acinetobacter species genotyping is required. A. calcoaceticus is a soil bacterium. It has been shown to be ... calcoaceticus to readily uptake DNA. Mechanismen der Regulation des pca-qui-Operons aus dem Bakterium Acinetobacter baylyi ...
Purification and characterization of quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus L.M.D. 79.41 P Dokter ... Quinoprotein glucose dehydrogenase (EC 1.1.99.17) from Acinetobacter calcoaceticus L.M.D. 79.41 was purified to homogeneity. It ... Cytochrome b -562 from Acinetobacter calcoaceticus L.M.D. 79.41. Its characteristics and role as electron acceptor for ... Reversible thermal inactivation of the quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus. Ca 2+ ions are ...
The antibiotic susceptibility of Acinetobacter calcoaceticus-Acinetobacter baumannii complex strains recovered from the ... Outbreak of imipenem-resistant Acinetobacter calcoaceticus-Acinetobacter baumannii complex harboring different carbapenemase ... Spread of imipenem-resistant Acinetobacter baumannii of European clone II in Western China. Chao He, Yi Xie, Hong Fan, Mei Kang ... Dissemination of imipenem-resistant Acinetobacter baumannii strains carrying the ISAba1 blaOXA-23 genes in a Chinese hospital. ...
... phylogenetic and multilocus sequence analyses from 975 strains/isolates of the Acinetobacter calcoaceticus/Acinetobacter ... Acinetobacter baumannii is nowadays a relevant nosocomial pathogen characterized by multidrug resistance (MDR) and concomitant ... Viale, Alejandro and Evans, Ben (2020) Microevolution in the major outer membrane protein OmpA of Acinetobacter baumannii. ... acinetobacter baumannii,ompa,outer membrane protein,protein evolution,recombination,epidemiology,microbiology,molecular biology ...
Retrieved from "https://en.wikipedia.org/w/index.php?title=Talk:Acinetobacter_calcoaceticus&oldid=587086953" ...
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sp,Q52110,MERD_ACICA HTH-type transcriptional regulator MerD OS=Acinetobacter calcoaceticus OX=471 GN=merD PE=4 SV=1 ... Acinetobacter calcoaceticus. ,p>This subsection of the ,a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names ... cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › ...
For many applications, A. calcoaceticus can be used as an alternative to Acinetobacter baumannii.. ... A. calcoaceticus Nutrient Agar. 37°C. Refrigerator. Tube. Each. Retrieving. This Item is temperature sensitive and has specific ...
Acinetobacter calcoaceticus (strepomycin sensitive) Domain: Prokaryote Optimal Growth Medium: Brain Heart Infusion Agar Optimal ... Genus and Species: Acinetobacter calcoaceticus (strepomycin sensitive) Domain: Prokaryote. Optimal Growth Medium: Brain Heart ... Acinetobacter calcoaceticus Strs, MicroKwik Culture®, Pathogen, Vial. Item # 154821A *bvseo_sdk, java_sdk, bvseo-4.0.0 ...
Infections with Acinetobacter calcoaceticus (Herellea vaginicola): clinical and laboratory studies.. Glew RH, Moellering RC Jr ... In a retrospective review of 53 patients, 58 episodes of infection due to Acinetobacter calcoaceticus var. anitratus (Herellea ... The severity of acinetobacter pneumonia is reflected in the high mortality rate (44% overall, with a 36% mortality rate due ... Skin infection due to A. calcoaceticus was seen in two patients, one of whom exhibited fulminant, fatal cellulitis and ...
Development of Colistin-Dependent Acinetobacter baumannii-Acinetobacter calcoaceticus Complex Joshua S. Hawley, Clinton K. ... Tetracycline Susceptibility Testing and Resistance Genes in Isolates of Acinetobacter baumannii-Acinetobacter calcoaceticus ... Colistin Heteroresistance in Acinetobacter and Its Association with Previous Colistin Therapy Joshua S. Hawley, Clinton K. ...
... regulates phenol degradation in Acinetobacter calcoaceticus; has ATP-binding activity; binds phenol; GenBank CAA93242 ... Acinetobacter calcoaceticus MopR protein: Member of the NtrC family of transcriptional activators with significant homology to ... Acinetobacter calcoaceticus MopR protein. Subscribe to New Research on Acinetobacter calcoaceticus MopR protein ... regulates phenol degradation in Acinetobacter calcoaceticus; has ATP-binding activity; binds phenol; GenBank CAA93242 ...
Genotypic and phenotypic characterization of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex with the proposal ... 13TU are increasingly recognized as clinically important taxa within the Acinetobacter calcoaceticus-Acinetobacter baumannii ( ... formerly Acinetobacter genomic species 3) and Acinetobacter nosocomialis sp. nov. (formerly Acinetobacter genomic species 13TU ... 13TU, we propose the formal names Acinetobacter pittii sp. nov. and Acinetobacter nosocomialis sp. nov. for these taxa, ...
... and Acinetobacter junii sp-nov and emended descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffii. Int. J. Syst ... gyrB Multiplex PCR To Differentiate between Acinetobacter calcoaceticus and Acinetobacter Genomic Species 3 Paul G. Higgins, ... Species-level identification of isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex by sequence ... gyrB Multiplex PCR To Differentiate between Acinetobacter calcoaceticus and Acinetobacter Genomic Species 3 ...
An endophytic bacterium Acinetobacter calcoaceticus Sasm3-enhanced phytoremediation of nitrate-cadmium compound polluted soil ... present study was to observe the effects of intercropping and inoculation of endophytic bacterium Acinetobacter calcoaceticus ...
Potential DNA slippage structures acquired during evolutionary divergence of Acinetobacter calcoaceticus chromosomal benABC and ... Potential DNA slippage structures acquired during evolutionary divergence of Acinetobacter calcoaceticus chromosomal benABC and ... Potential DNA slippage structures acquired during evolutionary divergence of Acinetobacter calcoaceticus chromosomal benABC and ... Potential DNA slippage structures acquired during evolutionary divergence of Acinetobacter calcoaceticus chromosomal benABC and ...
The nucleotide sequences of the Acinetobacter calcoaceticus benABC genes encoding a multicomponent oxygenase for the conversion ... Nucleotide sequences of the Acinetobacter calcoaceticus benABC genes for benzoate 1,2-dioxygenase reveal evolutionary ... Nucleotide sequences of the Acinetobacter calcoaceticus benABC genes for benzoate 1,2-dioxygenase reveal evolutionary ... Nucleotide sequences of the Acinetobacter calcoaceticus benABC genes for benzoate 1,2-dioxygenase reveal evolutionary ...
Acinetobacter calcoaceticus P23 is a plant growth-prom ... Acinetobacter calcoaceticus P23, in sterile medium and non- ...
... and genomic species 13TU included in the Acinetobacter calcoaceticus-Acinetobacter baumannii complex are genetically highly ... Except for A. calcoaceticus, they are all importa … ... of Isolates of the Acinetobacter calcoaceticus-Acinetobacter ... The species Acinetobacter calcoaceticus, A. baumannii, genomic species 3, ... Species-level Identification of Isolates of the Acinetobacter calcoaceticus-Acinetobacter Baumannii Complex by Sequence ...
Multidrug-resistant Acinetobacter baumannii-calcoaceticus complex has emerged as one of the most problematic pathogens in ... Endemicity of Acinetobacter calcoaceticus-baumannii complex in an intensive care unit in Malaysia. J Pathog. 2015;2015:789265. ... Carbapenem-nonsusceptible Acinetobacter baumannii-calcoaceticus complex carriage of included patients admitted to adult and ER- ... Endemic carbapenem-nonsusceptible Acinetobacter baumannii-calcoaceticus complex in intensive care units of the national ...
Acinetobacter calcoaceticus-Acinetobacter baumannii complex. bacteriophage. comparative genomics. depolymerase. capsule. Issue ... In this study, we isolated 12 phages infecting species of the Acinetobacter baumannii-Acinetobacter calcoaceticus complex which ... Ability of phages to infect Acinetobacter calcoaceticus-Acinetobacter baumannii complex species through acquisition of ... Ability of phages to infect Acinetobacter calcoaceticus-Acinetobacter baumannii complex species through acquisition of ...
... reliably between some closely related bacterial species including those of the Acinetobacter calcoaceticus-Acinetobacter ... baumannii (ACB) complex, namely A. baumannii and Acinetobacter nosocomialis. In the present study, we evaluated a protocol ... Discrimination of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex species by Fourier transform infrared ... Improvement of MALDI-TOF MS profiling for the differentiation of species within the Acinetobacter calcoaceticus-Acinetobacter ...
00695T0 from Acinetobacter calcoaceticus ruh2202. Plus protein sequence and external database links. ... Domain assignment for HMPREF0012_00695T0 from Acinetobacter calcoaceticus ruh2202. Domain architecture *Other proteins with ... HMPREF0012_00695 , Acinetobacter calcoaceticus RUH2202 ArtI protein (287 aa). Sequence. ...
Study of the range of antibody levels and activities of acinetobacter calcoaceticus-acinetobacter baumannii complex and ... Significance of the OXA-51-like β-lactamases of Acinetobacter baumannii.  Evans, Benjamin (The University of Edinburgh, 2010) ... Mobilome and antibiotic resistance in Acinetobacter baumannii  Opazo, Andres Felipe (The University of Edinburgh, 2014-11-28) ... The genus Acinetobacter currently contains 34 species, the vast majority of which are not regularly implicated in causing ...
A β-lactamase was purified 430-fold from the culture supernatant of Acinetobacter calcoaceticus by ion exchange chromatography ... Purification and characterization of an extracellular β-lactamase produced by Acinetobacter calcoaceticus * Bernd Blechschmidt ... A β-lactamase was purified 430-fold from the culture supernatant of Acinetobacter calcoaceticus by ion exchange chromatography ...
  • type II enzymes are basic proteins from which PQQ is not removed by dialysis against EDTA-containing buffers (examples are found in A. calcoaceticus and Gluconobacter oxydans). (portlandpress.com)
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