A species of gram-negative, aerobic bacteria, commonly found in the clinical laboratory, and frequently resistant to common antibiotics.
Infections with bacteria of the genus ACINETOBACTER.
A genus of gram-negative bacteria of the family MORAXELLACEAE, found in soil and water and of uncertain pathogenicity.
A group of beta-lactam antibiotics in which the sulfur atom in the thiazolidine ring of the penicillin molecule is replaced by a carbon atom. THIENAMYCINS are a subgroup of carbapenems which have a sulfur atom as the first constituent of the side chain.
A species of gram-negative, aerobic bacteria found in soil and water. Although considered to be normally nonpathogenic, this bacterium is a causative agent of nosocomial infections, particularly in debilitated individuals.
The ability of bacteria to resist or to become tolerant to several structurally and functionally distinct drugs simultaneously. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
Cyclic polypeptide antibiotic from Bacillus colistinus. It is composed of Polymyxins E1 and E2 (or Colistins A, B, and C) which act as detergents on cell membranes. Colistin is less toxic than Polymyxin B, but otherwise similar; the methanesulfonate is used orally.
Substances that reduce the growth or reproduction of BACTERIA.
Semisynthetic thienamycin that has a wide spectrum of antibacterial activity against gram-negative and gram-positive aerobic and anaerobic bacteria, including many multiresistant strains. It is stable to beta-lactamases. Clinical studies have demonstrated high efficacy in the treatment of infections of various body systems. Its effectiveness is enhanced when it is administered in combination with CILASTATIN, a renal dipeptidase inhibitor.
Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).
Any infection which a patient contracts in a health-care institution.
Enzymes found in many bacteria which catalyze the hydrolysis of the amide bond in the beta-lactam ring. Well known antibiotics destroyed by these enzymes are penicillins and cephalosporins.
Nonsusceptibility of bacteria to the action of the beta-lactam antibiotics. Mechanisms responsible for beta-lactam resistance may be degradation of antibiotics by BETA-LACTAMASES, failure of antibiotics to penetrate, or low-affinity binding of antibiotics to targets.
The ability of bacteria to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
A TETRACYCLINE analog, having a 7-dimethylamino and lacking the 5 methyl and hydroxyl groups, which is effective against tetracycline-resistant STAPHYLOCOCCUS infections.
Beta-lactam antibiotics that differ from PENICILLINS in having the thiazolidine sulfur atom replaced by carbon, the sulfur then becoming the first atom in the side chain. They are unstable chemically, but have a very broad antibacterial spectrum. Thienamycin and its more stable derivatives are proposed for use in combinations with enzyme inhibitors.
Gel electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length.
A beta-lactamase inhibitor with very weak antibacterial action. The compound prevents antibiotic destruction of beta-lactam antibiotics by inhibiting beta-lactamases, thus extending their spectrum activity. Combinations of sulbactam with beta-lactam antibiotics have been used successfully for the therapy of infections caused by organisms resistant to the antibiotic alone.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Proteins found in any species of bacterium.
Using MOLECULAR BIOLOGY techniques, such as DNA SEQUENCE ANALYSIS; PULSED-FIELD GEL ELECTROPHORESIS; and DNA FINGERPRINTING, to identify, classify, and compare organisms and their subtypes.
Hospital units providing continuous surveillance and care to acutely ill patients.
A technique for identifying individuals of a species that is based on the uniqueness of their DNA sequence. Uniqueness is determined by identifying which combination of allelic variations occur in the individual at a statistically relevant number of different loci. In forensic studies, RESTRICTION FRAGMENT LENGTH POLYMORPHISM of multiple, highly polymorphic VNTR LOCI or MICROSATELLITE REPEAT loci are analyzed. The number of loci used for the profile depends on the ALLELE FREQUENCY in the population.
Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.
DNA elements that include the component genes and insertion site for a site-specific recombination system that enables them to capture mobile gene cassettes.
Direct nucleotide sequencing of gene fragments from multiple housekeeping genes for the purpose of phylogenetic analysis, organism identification, and typing of species, strain, serovar, or other distinguishable phylogenetic level.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
The application of molecular biology to the answering of epidemiological questions. The examination of patterns of changes in DNA to implicate particular carcinogens and the use of molecular markers to predict which individuals are at highest risk for a disease are common examples.
Institutions with an organized medical staff which provide medical care to patients.
Bacteria which lose crystal violet stain but are stained pink when treated by Gram's method.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Infections caused by bacteria that show up as pink (negative) when treated by the gram-staining method.
Sudden increase in the incidence of a disease. The concept includes EPIDEMICS and PANDEMICS.
The functional hereditary units of BACTERIA.
Inflammation of the lung parenchyma that is caused by bacterial infections.
The presence of viable bacteria circulating in the blood. Fever, chills, tachycardia, and tachypnea are common acute manifestations of bacteremia. The majority of cases are seen in already hospitalized patients, most of whom have underlying diseases or procedures which render their bloodstreams susceptible to invasion.
Four-membered cyclic AMIDES, best known for the PENICILLINS based on a bicyclo-thiazolidine, as well as the CEPHALOSPORINS based on a bicyclo-thiazine, and including monocyclic MONOBACTAMS. The BETA-LACTAMASES hydrolyze the beta lactam ring, accounting for BETA-LACTAM RESISTANCE of infective bacteria.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
A method where a culturing surface inoculated with microbe is exposed to small disks containing known amounts of a chemical agent resulting in a zone of inhibition (usually in millimeters) of growth of the microbe corresponding to the susceptibility of the strain to the agent.
A broad-spectrum antibiotic derived from KANAMYCIN. It is reno- and oto-toxic like the other aminoglycoside antibiotics.
A mixture of polymyxins B1 and B2, obtained from Bacillus polymyxa strains. They are basic polypeptides of about eight amino acids and have cationic detergent action on cell membranes. Polymyxin B is used for infections with gram-negative organisms, but may be neurotoxic and nephrotoxic.
Monocyclic, bacterially produced or semisynthetic beta-lactam antibiotics. They lack the double ring construction of the traditional beta-lactam antibiotics and can be easily synthesized.
Ability of a microbe to survive under given conditions. This can also be related to a colony's ability to replicate.
Basic lipopeptide antibiotic group obtained from Bacillus polymyxa. They affect the cell membrane by detergent action and may cause neuromuscular and kidney damage. At least eleven different members of the polymyxin group have been identified, each designated by a letter.
Serious INFLAMMATION of the LUNG in patients who required the use of PULMONARY VENTILATOR. It is usually caused by cross bacterial infections in hospitals (NOSOCOMIAL INFECTIONS).
A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.
The genetic complement of a BACTERIA as represented in its DNA.
Any materials used in providing care specifically in the hospital.
A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.
Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.
Hospital department which manages and provides the required housekeeping functions in all areas of the hospital.
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
Low-molecular-weight compounds produced by microorganisms that aid in the transport and sequestration of ferric iron. (The Encyclopedia of Molecular Biology, 1994)
The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.
The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
Glycosylated compounds in which there is an amino substituent on the glycoside. Some of them are clinically important ANTIBIOTICS.
Proteins isolated from the outer membrane of Gram-negative bacteria.
Hospitals which provide care for the military personnel and usually for their dependents.
A semisynthetic antibiotic produced from Streptomyces mediterranei. It has a broad antibacterial spectrum, including activity against several forms of Mycobacterium. In susceptible organisms it inhibits DNA-dependent RNA polymerase activity by forming a stable complex with the enzyme. It thus suppresses the initiation of RNA synthesis. Rifampin is bactericidal, and acts on both intracellular and extracellular organisms. (From Gilman et al., Goodman and Gilman's The Pharmacological Basis of Therapeutics, 9th ed, p1160)
Distinct units in some bacterial, bacteriophage or plasmid GENOMES that are types of MOBILE GENETIC ELEMENTS. Encoded in them are a variety of fitness conferring genes, such as VIRULENCE FACTORS (in "pathogenicity islands or islets"), ANTIBIOTIC RESISTANCE genes, or genes required for SYMBIOSIS (in "symbiosis islands or islets"). They range in size from 10 - 500 kilobases, and their GC CONTENT and CODON usage differ from the rest of the genome. They typically contain an INTEGRASE gene, although in some cases this gene has been deleted resulting in "anchored genomic islands".
Methods for using more than one primer set in a polymerase chain reaction to amplify more than one segment of the target DNA sequence in a single reaction.
Specialized hospital facilities which provide intensive care for burn patients.
Programs of disease surveillance, generally within health care facilities, designed to investigate, prevent, and control the spread of infections and their causative microorganisms.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Simultaneous resistance to several structurally and functionally distinct drugs.
Lice of the genus Pediculus, family Pediculidae. Pediculus humanus corporus is the human body louse and Pediculus humanus capitis is the human head louse.
The functions, behavior, and activities of bacteria.
Hospitals maintained by a university for the teaching of medical students, postgraduate training programs, and clinical research.
A medical facility which provides a high degree of subspecialty expertise for patients from centers where they received SECONDARY CARE.
Gram-negative, non-motile, capsulated, gas-producing rods found widely in nature and associated with urinary and respiratory infections in humans.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
Invasion of the site of trauma by pathogenic microorganisms.
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
Bacterial infections of the leptomeninges and subarachnoid space, frequently involving the cerebral cortex, cranial nerves, cerebral blood vessels, spinal cord, and nerve roots.
Created 1 January 1993 as a result of the division of Czechoslovakia into the Czech Republic and Slovakia.
Technique that utilizes low-stringency polymerase chain reaction (PCR) amplification with single primers of arbitrary sequence to generate strain-specific arrays of anonymous DNA fragments. RAPD technique may be used to determine taxonomic identity, assess kinship relationships, analyze mixed genome samples, and create specific probes.

Distribution of beta-lactamases in Acinetobacter baumannii clinical isolates and the effect of Syn 2190 (AmpC inhibitor) on the MICs of different beta-lactam antibiotics. (1/853)

The distribution of beta-lactamases in a group of 20 epidemiologically well defined Acinetobacter baumannii clinical isolates and the in vitro activity of Syn 2190, a novel beta-lactamase AmpC inhibitor, were determined. Twenty-five per cent of the strains carried and expressed a TEM-type beta-lactamase, whereas 35% had an OXA-type beta-lactamase. In nine out of 11 (82%) ceftazidime-resistant and four out of 13 (30.7%) cefepime-resistant strains, the MIC of these beta-lactam antibiotics decreased when determined in the presence of Syn 2190. Thus, our results suggest that in a high percentage of A. baumannii clinical isolates the increased production of AmpC, in combination or not with other resistance mechanisms, contributes to the resistance pattern in A. baumannii to beta-lactams.  (+info)

Molecular characterization of integrons in epidemiologically unrelated clinical isolates of Acinetobacter baumannii from Italian hospitals reveals a limited diversity of gene cassette arrays. (2/853)

Integron carriage by 36 epidemiologically unrelated Acinetobacter baumannii isolates collected over an 11-year period from patients in six different Italian hospitals was investigated. Sixteen type 1 integron-positive isolates (44%) were found, 13 of which carried the same array of cassettes, i.e., aacC1, orfX, orfX', and aadA1a. As ribotype analysis of the isolates demonstrated a notable genetic diversity, horizontal transfer of the entire integron structure or ancient acquisition was hypothesized.  (+info)

Genetic and phenotypic analysis of Acinetobacter baumannii insertion derivatives generated with a transposome system. (3/853)

Acinetobacter baumannii is a metabolically versatile pathogen that causes severe infections in compromised patients. However, little is known about the genes and factors involved in its basic physiology and virulence properties. Insertion mutagenesis was used to initiate the identification and characterization of some of these factors and genes in the prototype strain 19606. The utilization of the pLOFKm suicide delivery vector, which harbors a suicide mini-Tn10 derivative, proved to be unsuccessful for this purpose. The EZ::TN Tnp transposome system available from Epicentre was then used in conjunction with electroporation to generate isogenic insertional derivatives of A. baumannii 19606. Replica plating showed that 2% of the colonies that grew after electroporation on agar plates without antibiotics also grew in the presence of 40 micro g of kanamycin per ml. DNA hybridization proved that all of the kanamycin-resistant derivatives contained the EZ::TN insertion element, which was mapped to different genomic locations. Replica plating on Simmons citrate agar and microtiter plate-plastic tube assays identified growth- and biofilm-defective derivatives, respectively. The location of the insertion in several of these derivatives was determined by self-ligation of NdeI- or EcoRI-digested genomic DNA and electroporation of Escherichia coli TransforMax EC100D (pir(+)). Sequence analysis of the recovered plasmids showed that some of the A. baumannii 19606 growth-defective derivatives contain insertions within genes encoding activities required for the generation of energy and cell wall components and for the biosynthesis of amino acids and purines. A gene encoding a protein similar to the GacS sensor kinase was interrupted in four derivatives, while another had an insertion in a gene coding for a hypothetical sensor kinase. A. baumannii 19606 derivatives with defective attachment or biofilm phenotypes had insertions within genes that appear to be part of a chaperone-usher transport system described for other bacteria. DNA hybridization experiments showed that the presence of strain 19606 genes encoding regulatory and attachment or biofilm functions is widespread among other A. baumannii clinical isolates.  (+info)

Comparison of a repetitive extragenic palindromic sequence-based PCR method and clinical and microbiological methods for determining strain sources in cases of nosocomial Acinetobacter baumannii bacteremia. (4/853)

Using a repetitive extragenic palindromic PCR (REP-PCR), we genotypically characterized strains causing nosocomial Acinetobacter baumannii infections and analyzed the source of bacteremia in 67 patients from an institution in which infections by this bacterium were endemic. Six different genotypes were found, including 21, 27, 3, 9, 3, and 4 strains. The probable source of bacteremia, according to clinical and/or microbiological criteria, was known in 42 patients (63%): respiratory tract (n = 19), surgical sites (n = 12), intravascular catheters (n = 5), burns (n = 3), and urinary tract (n = 3). The definite source of bacteremia, according to REP-PCR, could be established in 30 (71%) out of the 42 patients with strains from blood and other sites; in these cases clinical and microbiological criteria for the source of bacteremia were thus confirmed. In the remaining 12 patients (29%) the probable source was refuted by the REP-PCR method. The definite sources of bacteremia according to genotype were as follows: respiratory tract in 13 patients (31%), surgical sites in 8 (19%), intravascular catheters in 4 (9%), burns in 3 (7%), and urinary tract in 2 (5%). A comparison of strains from blood cultures and other sites with regard to their REP-PCR and antimicrobial resistance profiles was also made. Taking the REP-PCR as the "gold standard," the positive predictive value of antibiotype was 77% and the negative predictive value was 42%. In summary, the utility of the diagnosis of the source of nosocomial A. baumannii bacteremia using clinical and/or microbiological criteria, including antibiotyping, is limited, as demonstrated by REP-PCR.  (+info)

Endemic carbapenem resistance associated with OXA-40 carbapenemase among Acinetobacter baumannii isolates from a hospital in northern Spain. (5/853)

Eighty-two carbapenem-resistant isolates of Acinetobacter baumannii from a single hospital in Bilbao were typed into two major clusters and several subclusters. Disk synergy tests and PCR indicated the production of a zinc-independent OXA-class carbapenemase. Sequencing identified this enzyme, OXA-40, as a variant of the OXA-24-OXA-25-OXA-26 cluster.  (+info)

Loss of a 29-kilodalton outer membrane protein in Acinetobacter baumannii is associated with imipenem resistance. (6/853)

We analyzed the possible causes of imipenem (IPM) resistance in multidrug-resistant isolates of Acinetobacter baumannii. Comparison of the outer membrane protein (OMP) profiles of two genomically related strains (Ab288 [IPM sensitive] and Ab242 [IPM resistant]) indicated the conspicuous loss of a 29-kDa polypeptide in the Ab242 strain. No carbapenemase activity was detected in any of these strains. The treatment of Ab288 with sodium salicylate resulted in IPM resistance and the loss of the 29-kDa OMP. In addition, IPM-resistant clones of Ab288 which were selected by repetitive culturing in increasing concentrations of this antibiotic also showed the absence of this 29-kDa OMP.  (+info)

Genetic and functional analysis of the chromosome-encoded carbapenem-hydrolyzing oxacillinase OXA-40 of Acinetobacter baumannii. (7/853)

Clinical isolate Acinetobacter baumannii CLA-1 was resistant to a series of antibiotic molecules, including carbapenems. Cloning and expression of the beta-lactamase gene content of this isolate in Escherichia coli DH10B identified a chromosome-encoded oxacillinase, OXA-40, that differed by one or two amino acid changes from OXA-24, -25, and -26 and an AmpC-type cephalosporinase. The OXA-40 beta-lactamase had a mainly narrow-spectrum hydrolytic profile, but it included ceftazidime and imipenem. Its activity was resistant to inhibition by clavulanic acid, tazobactam, sulbactam, and, like most of the other carbapenem-hydrolyzing oxacillinases, NaCl. OXA-40 had an FGN triad replacing a YGN motif at class D beta-lactamase (DBL) positions 144 to 146. Site-directed DNA mutagenesis leading to a Phe-to-Tyr change at DBL position 144 in OXA-40 gave a mutant enzyme with increased hydrolytic activity against most beta-lactams, including imipenem. Conversely, with a gene encoding the narrow-spectrum oxacillinase OXA-1 as the template, a nucleotide substitution leading to a Tyr-to-Phe change in the YGN motif of OXA-1 gave a mutant enzyme with decreased hydrolytic activity without an increase in carbapenem-hydrolyzing activity. Thus, the Phe residue in the FGN motif was not associated with carbapenem-hydrolyzing activity by itself but instead was associated with weak overall hydrolytic activity. Finally, this Phe residue in OXA-40 explained resistance to inhibition by NaCl whereas a Tyr residue in motif YGN was related to susceptibility to NaCl.  (+info)

Relationship between beta-lactamase production, outer membrane protein and penicillin-binding protein profiles on the activity of carbapenems against clinical isolates of Acinetobacter baumannii. (8/853)

Twenty blood isolates of Acinetobacter baumannii were studied, representing eight pulsed-field gel electrophoresis patterns and all different antimicrobial susceptibility patterns observed during 1995-97 at the University Hospital Virgen Macarena, Seville, Spain. The MIC(90)s (mg/L) of imipenem and meropenem decreased from 16 to 0.5 and from 8 to 4, respectively, in the presence of BRL 42715 (BRL) but not clavulanic acid. Hydrolysing activity (nmol/min/mg) of bacterial supernatants against cefaloridine ranged from 8.8 to 552.3 for A. baumannii type I (imipenem MICs < or = 2), which expressed only a beta-lactamase of pI > or = 9, and from 12.3 to 1543.5 for A. baumannii type II (imipenem MICs > or = 4), which expressed a beta-lactamase of pI > or = 9 and two others of pI 6.3 and 7. The hydrolysing activities of A. baumannii type II against imipenem, meropenem and oxacillin were higher than those observed for A. baumannii type I. Ten outer membrane protein (OMP) profiles (A. baumannii types I and II) were visualized on 10% SDS-PAGE gels with 6 M urea, whereas only five OMP profiles (A. baumannii types I and II) were differentiated in 12% SDS-PAGE gels. Five A. baumannii with OMP profile type B, characterized by the absence of a 22.5 kDa OMP, were resistant to meropenem and/or imipenem. Twelve penicillin-binding protein (PBP) patterns were observed. PBP patterns of A. baumannii type II were characterized by the absence of a 73.2 kDa band (PBP 2). We concluded that production of beta-lactamases of pI 6.3 and 7.0 and reduced expression of PBP 2 are the most frequently observed mechanisms of resistance to carbapenems. In some isolates, loss of a 22.5 kDa OMP is also related to resistance to carbapenems.  (+info)

TY - JOUR. T1 - Environmental exposure to carbapenem-resistant Acinetobacter baumannii as a risk factor for patient Acquisition of A. baumannii. AU - Rosa, Rossana. AU - Arheart, Kristopher L.. AU - Depascale, Dennise. AU - Cleary, Timothy. AU - Kett, Daniel H.. AU - Namias, Nicholas. AU - Pizano, Louis. AU - Fajardo-Aquino, Yovanit. AU - Silvia Munoz-Price, L.. PY - 2014. Y1 - 2014. N2 - We aimed to determine the association between environmental exposure to carbapenem-resistant Acinetobacter baumannii and the subsequent risk of acquiring this organism. Patients exposed to a contaminated hospital environment had 2.77 times the risk of acquiring carbapenem-resistant A. baumannii than did unexposed patients (relative risk, 2.77 [95% confidence interval, 1.50-5.13]; P p.002).. AB - We aimed to determine the association between environmental exposure to carbapenem-resistant Acinetobacter baumannii and the subsequent risk of acquiring this organism. Patients exposed to a contaminated hospital ...
Objectives. Multidrug-resistant Acinetobacter strain HK302 was isolated from an outbreak of nosocomial infections in Switzerland in 1977. The aim of the present study was to assess whether this archive strain belongs to one of the known international clonal lineages of Acinetobacter baumannii and whether it harbours a genomic structure related to the AbaR1-like resistance islands.. Methods. Multilocus sequence typing (MLST) and HindIII ribotyping were used to determine the taxonomic position of HK302 at the species and subspecies (clonal) levels. The position and structure of the putative resistance island were investigated by AbaR1-based PCR mapping followed by restriction analysis and partial sequencing of amplicons. A. baumannii AYE harbouring AbaR1 was used as a positive control for PCR mapping.. Results. The MLST allelic profile (1-1-1-1-5-1-1) and HindIII ribotype of HK302 were typical of A. baumannii European (EU) clone I. In addition, an AbaR1-related region inserted into the ATPase gene ...
Carbapenem resistance in Acinetobacter baumannii is a global problem. The purpose of this study was to elucidate current resistance mechanisms of imipenem-resistant A. baumannii (IRAB) in Taiwan and their correlation with patient outcomes. Acinetobacter baumannii clinical isolates from two teaching hospitals in Taiwan were collected in 2009 and were examined by Etest for determination of the minimum inhibitory concentrations (MICs) of imipenem, ceftazidime and ceftriaxone. Primers specific for carbapenemase genes and upstream regions were designed for PCR amplification. Bacterial isolates were genotyped by pulsed-field gel electrophoresis (PFGE). Clinical presentations of patients were analysed retrospectively. Upstream insertion sequence ISAba1 was found in 34 isolates that carried bla(OXA-23), including 28 with transposon Tn2006 (ISAba1-bla(OXA-23)-ISAba1) in an AbaR4-type resistance island and 6 with Tn2008 (ISAba1-bla(OXA-23)), as well as in 8 isolates carrying ISAba1-bla(OXA-51-like). All ...
OBJECTIVE: The aim of this multiple-hospital study was to investigate the prevalence of integrons in multidrug-resistant Acinetobacter baumannii (MDRAB) in Eastern China, and characterize the integron-integrase genes, so as to provide evidence for the management and appropriate antibiotic use of MDRAB infections.. METHODS: A total of 425 clinical isolates of A. baumannii were collected from 16 tertiary hospitals in 11 cities of four provinces (Fujian, Jiangsu, Zhejiang and Shandong) from January 2009 to June 2012. The susceptibility of A. baumannii isolates to ampicillin/sulbactam, piperacillin/tazobactam, ceftazidime, ceftriaxone, cefepime, aztreonam, meropenem, amikacin, gentamicin, tobramycin, ciprofloxacin, levofloxacin, sulfamethoxazole/trimenthoprim, minocycline and imipenem was tested, and integrons and their gene cassettes were characterized in these isolates using PCR assay. In addition, integron-positive A. baumannii isolates were genotyped using pulsed-field gel electrophoresis (PFGE) ...
Nosocomial pathogens can be associated with a variety of infections, particularly in intensive care units (ICUs) and in immunocompromised patients. Usually these pathogens are resistant to multiple drugs and pose therapeutic challenges. Among these organisms, Acinetobacter baumannii is one of the most frequent being encountered in the clinical setting. Carbapenems are very useful to treat infections caused by these drug-resistant Gram-negative bacilli, but carbapenem resistance is increasing globally. Combination therapy is frequently given empirically for hospital-acquired infections in critically ill patients and is usually composed of an adequate beta-lactam and an aminoglycoside. The purpose of this study was to evaluate the in vitro activity of plazomicin against carbapenem-resistant Acinetobacter baumannii. Amikacin was used as a comparator. The activity of plazomicin in combination with several different antibiotics was tested by disk diffusion, the checkerboard method, and time-kill ...
Acinetobacter baumannii is a nosocomial pathogen which is establishing as a major cause of morbidity and mortality within the healthcare community. The success of this pathogen is largely due to its ability to rapidly gain resistance to antimicrobial therapies and its capability to persist in an abiotic environment through the production of a biofilm. Our tertiary-care hospital has showed high incidence of carbapenem-resistant Acinetobacter baumannii (CRAB) isolates. In this study we explore both genotypic and phenotypic properties of 26 CRAB isolates: 16 isolates were collected from January 2010 to March 2011, and 10 were collected between February and May 2015. We determined that all 26 CRAB isolates possessed multiple β-lactamase genes, including genes from Groups A, C, and D. Specifically, 42% of the isolates possesses the potentially plasmid-borne genes of OXA-23-like or OXA-40-like β-lactamase. The presence of mobile gene element integron cassettes and/or integrases
Carbapenem-resistant Acinetobacter baumannii is the top-ranked pathogen in the World Health Organization priority list of antibiotic-resistant bacteria. It emerged as a global pathogen due to the successful expansion of a few epidemic lineages, or international clones (ICs), producing acquired class D carbapenemases (OXA-type). During the past decade, however, reports regarding IC-I isolates in Latin America are scarce and are non-existent for IC-II and IC-III isolates. This study evaluates the molecular mechanisms of carbapenem resistance and the epidemiology of 80 non-duplicate clinical samples of A. baumannii collected from February 2014 through April 2016 at two tertiary care hospitals in Lima. Almost all isolates were carbapenem-resistant (97.5%), and susceptibility only remained high for colistin (95%). Pulsed-field gel electrophoresis showed two main clusters spread between both hospitals: cluster D containing 51 isolates (63.8%) associated with sequence type 2 (ST2) and carrying OXA-72, ...
Multidrug-resistant Acinetobacter baumannii infection has presented a global medical challenge. The antibiograms of paired colistin-susceptible and -resistant strains revealed increased susceptibility of colistin-resistant strains to most tested antibiotics, including those that are active against only gram-positive bacteria. Synergy between colistin and rifampicin was observed in the colistin-susceptible strains. The ability to form biofilm in the colistin-resistant strains was significantly lower (P , .001) than in the parent strains. Our study provides valuable information for potential expansion of our current therapeutic options against colistin-resistant A. baumannii infection.. ...
Carbapenem-resistant Acinetobacter baumannii is becoming increasingly prevalent in patients with diabetes mellitus in the Middle East. We examined the relationship of these bacteria and their resistance mechanisms to the diabetic disease status of patients in Saudi Arabia. Susceptibilities of 271 isolates to carbapenems, tigecycline and colistin were determined, followed by detection of carbapenemase genes. A blaVIM gene was detected in ~95 % of isolates; blaOXA-23 and blaOXA-40 genes were also prevalent. Diabetic patients were significantly more likely to carry carbapenem-resistant isolates. Carbapenem-resistant A. baumannii is a serious problem in diabetic patients, and molecular detection of resistance mechanisms in these isolates is required.. ...
TY - CHAP. T1 - Desiccation tolerance assays for acinetobacter baumannii. AU - Wang, Xun. AU - Trent, M. Stephen. AU - Davies, Bryan William. PY - 2019/1/1. Y1 - 2019/1/1. N2 - Acinetobacter baumannii is a hospital-associated pathogen of growing importance and is a paradigm for endemic hospital contamination. Desiccation tolerance has been implicated as an important characteristic that potentiates the spread of A. baumannii in clinical settings through contaminated healthcare equipment and personnel. Desiccation is a multifaceted stress, and many physiological and environmental factors can influence its impact on bacterial survival. This chapter provides a protocol for assessing desiccation survival that facilitates comparisons among A. baumannii strains under various environmental conditions.. AB - Acinetobacter baumannii is a hospital-associated pathogen of growing importance and is a paradigm for endemic hospital contamination. Desiccation tolerance has been implicated as an important ...
Carbapenem resistance in A. baumannii is most often associated with class D β-lactamases (OXA-23-like, OXA-40-like and OXA-58-like) and MBLs. OXA-type carbapenemases are predominant in A. baumannii, particularly in worldwide outbreaks of OXA-23 [24]. The molecular analysis of the isolates tested in this study revealed that 14 strains (51.8 %) carried the blaOXA-23-like gene and that two strains carried a blaOXA-24-like gene. All of the strains had a blaOXA-51-like gene, and four strains had a blaOXA-58 gene. In this study, the OXA-58 isolates presented lower MIC values for meropenem than OXA-23-like-positive isolates, which systematically exhibited higher MIC values (Table 1). The isolates with non-acquired OXA genes displayed a marked variation and included some carbapenem-resistant genes. Naturally occurring OXA carbapenemases, such as OXA-51-like enzymes (e.g., OXA 64-66, OXA 68-71, OXA 78-80, OXA-82, OXA-86, OXA-92 and OXA104-112), have been identified in A. baumannii isolates worldwide. In ...
TY - JOUR. T1 - Variations in IS6 promoters alter the expression of carbapenem resistance in related strains of Acinetobacter baumannii. AU - Al-Hassan, Leena. AU - Opazo, Andres. AU - Lopes, Bruno S. AU - Mahallawy, Hadir El. AU - Amyes, Sebastian G B. N1 - The authors are thankful to the hospital staff at The Childrens Cancer Hospital and The National Cancer Institute (Cairo, Egypt) for providing the samples and allowing part of the work to be undertaken at their centres.. PY - 2015/3. Y1 - 2015/3. N2 - The aim of this work was to investigate the role of the IS6 family of insertion sequences present upstream of blaOXA-58 in two clonally related carbapenem-resistant Acinetobacter baumannii isolates obtained from paediatric cancer patients in Egypt. To determine their relatedness, the isolates were typed by pulsed-field gel electrophoresis (PFGE), and the intrinsic blaOXA-51-like gene was amplified and sequenced. Minimum inhibitory concentrations (MICs) to imipenem and meropenem was determined ...
Acinetobacter baumannii has been increasingly reported in the outbreak of nosocomial infections in the intensive care units, which not only prolong the length of hospital stay but result in high attributable mortality. With its intrinsic resistance to many antimicrobial agents and rapid acquirement of resistance mechanism, resistance to carbapenems, which is often accompanied with resistance to multiple drugs, has emerged worldwide. The limited treatment choice included tigecycline, colistin, and sulbactam. However, the low serum level and bacteriostatic nature of tigecycline hamper its application in blood stream infection, one of the most common presentations of A. baumannii infections. The nephrotoxicity and neurotoxicity of intravenous colistin have caused great concerns in critically ill patients whereas immediate bronchospasm after inhalation and significant clinical consequences have been reported. Sulbactam has been used for decades in combination of ampicillin and well tolerated. ...
Acinetobacter baumannii ATCC ® 43498™ Designation: S2 TypeStrain=False Application: Assay of cefoperazone Assay of sulbactam Quality control of cefoperazone/sulbactam
TY - JOUR. T1 - Integron-associated imipenem resistance in Acinetobacter baumannii isolated from a regional hospital in Taiwan. AU - Liu, S. Y.. AU - Lin, J. Y.. AU - Chu, C.. AU - Su, L. H.. AU - Lin, T. Y.. AU - Chiu, C. H.. PY - 2006/1. Y1 - 2006/1. N2 - We investigated the genetic properties of imipenem-resistant Acinetobacter baumannii collected from a regional hospital in Taiwan. Pulsed-field gel electrophoresis demonstrated that the isolates were genetically diverse. Polymerase chain reaction, DNA sequencing, and DNA-DNA hybridisation showed that the blaIMP-1 gene resided as a cassette in a plasmid-borne class 1 integron in two isolates. The majority of the resistant isolates were plasmid-less and carried no blaIMP, blaVIM or bla CFI genes, indicating that other uncharacterised metallo-β- lactamases or mechanisms other than enzyme production are involved in carbapenem resistance in this group of A. baumannii. We conclude that multidrug resistance of A. baumannii was a combined effect of ...
In February 2006, a patient colonized with a multidrug-resistant sequence type 56 Acinetobacter baumannii strain was admitted to a hospital in Madrid, Spain. This strain spread rapidly and caused a large outbreak in the hospital. Clinicians should be ...
PubMedID: 24985124 | Successful management of an outbreak due to carbapenem-resistant Acinetobacter baumannii in a neonatal intensive care unit. | European journal of pediatrics | 7/2/2014
Identifying Risk Factors for Healthcare-Associated Infections Caused by Carbapenem-Resistant Acinetobacter baumannii in a Neonatal Intensive Care Unit
The traditional markerless gene deletion technique based on overlap extension PCR has been used for generating gene deletions in multidrug-resistant Acinetobacter baumannii. However, the method is time-consuming because it requires restriction digestion of the PCR products in DNA cloning and the con …
Acinetobactin is a major siderophore utilized by the human pathogen Acinetobacter baumannii. The rapid acquisition of drug resistance by A. baumannii has garnered concern globally. Herein, acinetobactin and systematically generated analogues were prepared and characterized; the binding and cellular delivery of Fe(III) by the analogues were evaluated. This investigation not only led to the clarification of the physiologically relevant acinetobactin structure but also revealed several key structural elements for its functionality as a siderophore.. ...
Acinetobacter baumannii is nowadays a relevant nosocomial pathogen characterized by multidrug resistance (MDR) and concomitant difficulties to treat infections. OmpA is the most abundant A. baumannii outer membrane (OM) protein, and is involved in virulence, host-cell recognition, biofilm formation, regulation of OM stability, permeability and antibiotic resistance. OmpA members are two‐domain proteins with an N‐terminal eight‐stranded β‐barrel domain with four external loops (ELs) interacting with the environment, and a C‐terminal periplasmic domain binding non‐covalently to the peptidoglycan. Here, we combined data from genome sequencing, phylogenetic and multilocus sequence analyses from 975 strains/isolates of the Acinetobacter calcoaceticus/Acinetobacter baumannii complex (ACB), 946 from A. baumannii, to explore ompA microevolutionary divergence. Five major ompA variant groups were identified (V1 to V5) in A. baumannii, encompassing 52 different alleles coding for 23 different ...
Acinetobacter baumannii bacteremia is becoming more prevalent and is associated with increasing morbidity and mortality. Escalating antibacterial resistance further contributes to therapeutic dilemmas, enhanced infection control support and poorer outcomes in patients infected with these bacteria. A retrospective analysis of patients whose blood cultures produced A. baumannii from January 2007 through January 2013 was performed. Data regarding the epidemiologic features, antimicrobial susceptibility and outcomes of patients with A. baumannii bacteremia were collected and analyzed. Sixty A. baumannii isolates each from a different patient were identified. The Charlson Comorbidity Index (≥3) was the greatest among patients with multi-drug resistance (MDR) compared to intermediate drug resistance (IDR) and pan-sensitive (PS) A. baumannii. The mean APACHE II scores for MDR, IDR and PS A. baumannii bacteremia were 21, 15 and 11, respectively (P < 0.05, MDR v. PS). Seventy-three percent of the isolates were
Acinetobacter baumannii causes severe nosocomial infections such as pneumonia, meningitis and sepsis with high mortality rates. This organism represents an increasing danger for immunocompromised adults, especially since there are an increasing number of resistances against antibiotics. Until now, scientific investigation was mainly focused on taxonomy and antibiotic resistance mechanisms. The goal of this project was to analyse the interaction between clinical strains of Acinetobacter baumannii and human cells in order to address the molecular mechanisms causing pathogenicity. Adherence is the first step in colonization of human tissue, and therefore a key event in pathogenesis. To demonstrate the adhesion of bacteria to human cells, a colony counting assay has been established. These experiments used the the type strain of A. baumannii ATCC 19606, as well as clinical isolates. All A. baumannii strains investigated showed adhesion to the lung epithelial cells A549, but the adhesion capacity was ...
Acinetobacter baumannii ATCC ® 19606D-5™ Designation: Genomic DNA from Acinetobacter baumannii strain 2208 TypeStrain=True Application: Food testing
Fig. 1. (a) PFGE analysis of Acinetobacter baumannii strains. (b) Plasmid identification by digestion with S1 nuclease. (c) Hybridization with blaNDM-1 probe. Lanes: 1, A. baumannii AB-I1; 2, AB-I2; 3, AB-I3; 4, AB-I4; 5, AB-I5. Lanes 6 to 8, A. baumannii European clones EC-I (strain RUH-875), EC-II (strain RUH-134), and EC-III (strain RUH-5875), respectively. Bands with white arrows indicate the presence of plasmids without signal hybridization with the blaNDM-1 probe; black arrow indicates the chromosomal position with positive hybridization with the blaNDM-1 probe. ...
Acinetobacter baumannii is currently one of the key nosocomial pathogens causing severe infections; of special concern is its resistance to expanded-spectrum cephalosporins (ESCs) and carbapenems, often associated with the few so-called European clones (6, 7, 19). It has two natural -lactamases, an AmpC-like enzyme (Acinetobacter-derived cephalosporinase [ADC]) (10) and a carbapenem-hydrolyzing class D -lactamase (CHDL; the OXA-51 type) (15), which affect susceptibility upon increased expression due to ISAba1 insertion upstream of their genes (9, 18). Moreover, acquired -lactamases, including metallo-lactamases (MBLs) and four CHDL types, the OXA-23, OXA-24/40, OXA-58, and OXA-143 types, are observed (15). Knowledge of A. baumannii in Poland has been limited to single isolates (9, 14, 21); our aim was to analyze a bigger group of A. baumannii strains. (Part of this work was presented at the 22nd European Congress of Clinical Microbiology and Infectious Diseases, London, United Kingdom, 31 March to 3
To understand the epidemiology of multidrug-resistant (MDR) Acinetobacter baumannii and define individual risk factors for MDR, we used epidemiologic methods, performed organism typing by pulsed-field gel electrophoresis (PFGE), and conducted a matched case-control retrospective study. We investigated 118 patients, on 27 wards, in whom MDR A. baumannii was isolated from clinical cultures. Each case-patient had a control without MDR A. baumannii and was matched for hospital length of stay, ward, and calendar time. The epidemiologic investigation found small clusters of up to 6 patients each with no common identified source. Ten different PFGE clones were found, of which 2 dominated. The PFGE pattern differed within temporospatial clusters, and antimicrobial drug susceptibility patterns varied within and between clones. Multivariate analysis identified the following significant risk factors: male sex, cardiovascular disease, having undergone mechanical ventilation, and having been treated with
Hospital-acquired infections due to Acinetobacter baumannii have become problematic because of high rates of drug resistance. A. baumannii is usually harmless, but it causes sepsis resulting in a high mortality rate in compromised hosts. Therefore, we must consider its interaction with host cells to understand diseases resulting from A. baumannii infection. Neutrophils play a critical role in infective protection against the extracellular growth of bacteria. However, their interactions with A. baumannii remain largely unknown. Recently, a new biological defense mechanism called neutrophil extracellular traps (NETs) has been attracting attention. In the present study, we investigated the responsiveness of human neutrophils to A. baumannii focusing on NET formation. The results demonstrated that infective protection against Pseudomonas aeruginosa via NETs formation was observed, but for A. baumannii NETs formation did not occur. It seems that the innate infective protection against A. baumannii ...
TY - JOUR. T1 - Preclinical advantages of intramuscularly administered peptide A3-APO over existing therapies in Acinetobacter baumannii wound infections. AU - Ostorhazi, Eszter. AU - Rozgonyi, Ferenc. AU - Sztodola, Andras. AU - Harmos, Ferenc. AU - Kovalszky, Ilona. AU - Szabo, Dora. AU - Knappe, Daniel. AU - Hoffmann, Ralf. AU - Cassone, Marco. AU - Wade, John D.. AU - Bonomo, Robert A.. AU - Otvos, Laszlo. PY - 2010/9/1. Y1 - 2010/9/1. N2 - Objectives: The designer antibacterial peptide A3-APO is efficacious in mouse models of Escherichia coli and Acinetobacter baumannii systemic infections. Here we compare the efficacy of the peptide with that of imipenem and colistin in A. baumannii wound infections after burn injury. Methods: CD-1 mice were inflicted with burn wounds and different inocula of A. baumannii, isolated from an injured soldier, were placed into the wound sites. The antibiotics were given intramuscularly (im) one to five times. Available free peptide in the blood and the ...
Sigma-Aldrich offers abstracts and full-text articles by [Danielle M Stacy, Michael A Welsh, Philip N Rather, Helen E Blackwell].
Introduction: Acinetobacter baumannii is opportunistic in debilitated hospitalised patients. Because information from some South American countries was previously lacking, this study examined the emergence of multi-resistant A. baumannii in three hospitals in Cochabamba, Bolivia, from 2008 to 2009. Methodology: Multiplex PCR was used to identify the main resistance genes in 15 multi-resistant A. baumannii isolates. RT-PCR was used to measure gene expression. The genetic environment of these genes was also analysed by PCR amplification and sequencing. Minimum inhibitory concentrations were determined for key antibiotics and some were determined in the presence of an efflux pump inhibitor, 1-(1-napthylmethyl) piperazine. Results: Fourteen strains were found to be multi-resistant. Each strain was found to have the bla(OXA-58) gene with the ISAba3-like element upstream, responsible for over-expression of the latter and subsequent carbapenem resistance. Similarly, ISAba1, upstream of the bla(ADC) ...
Acinetobacter baumannii is a strain of bacteria in the Acinetobacter genus. This genome was published to the ATCC Genome Portal on 2020-08-03
Construction of Integrated Analytic Platform for Acinetobacter Baumannii in Taiwan-Network Analysis and Comparative Genomics Study on Acinetobacter Baumannii Isolates from Taiwan(2/3 ...
Objective To investigate the in vitro and in vivo antibacterial activities of tigecycline and other 13 common antimicrobial agents, alone or in combination, against multi-drug resistant Acinetobacter baumannii.MethodsAn in vitro susceptibility test of 101 Acinetobacter baumannii was used to detect minimal inhibitory concentrations (MICs). A mouse lung infection model of multi-drug resistant Acinetobacter baumannii,established by the ultrasonic atomization method, was used to define in vivo antimicrobial activities.Results Multi-drug resistant Acinetobacter baumannii showed high sensitivity to tigecycline (98% inhibition), polymyxin B (78.2% inhibition), and minocycline (74.2% inhibition). However, the use of these antimicrobial agents in combination with other antimicrobial agents produced synergistic or additive effects. In vivo data showed that white blood cell (WBC) counts in drug combination groups C (minocycline + amikacin) and D (minocycline + rifampicin) were significantly higher than in groups A
Patients with AB bacteremia receiving antimicrobial therapy are eligible for this multicenter study. Antimicrobial agents are decided at the discretion of the attending clinical team. Clinical data to be collected include patient demographics (age, gender, underlying diseases, Pitt Bacteremia Score [20], duration of ICU stay and hospitalization before the day of first positive blood culture, central venous catheterization), antimicrobial agents on the day of bacteremia, regimens and durations of combination therapy after enrollment, and outcomes (sequential quantification change of blood A. baumannii polymerase chain reaction [PCR], survival at day 30 after enrollment, and adverse drug reactions of antimicrobial agents). Blood sample will be collected on the day of enrollment (Day 0), Day 1, 2, 3 and 7 for PCR quantification of A. baumannii and for genospecies identification. Primary end points are the interval from study enrollment to negative blood A. baumannii PCR and blood sterilization. ...
In recent years, the number of nosocomial infections caused byAcinetobacter baumannii has increased significantly (4). Many outbreaks have been reported, especially among patients confined to hospital intensive care units, where the widespread use of antibiotics may select multidrug-resistant strains. The difficulty of treating A. baumannii nosocomial infection is associated with the high resistance to a wide range of antimicrobial agents frequently observed in this species (8). Often, imipenem remains one of the few therapeutic alternatives. Fortunately, imipenem resistance is relatively rare among Acinetobacter clinical isolates. Carbapenem resistance can arise by a decrease in expression of an outer membrane protein (3) or by alteration in penicillin-binding proteins (5). In general, the emergence of carbapenem-hydrolyzing enzymes has been limited compared to the prevalence of other β-lactamases (1). However, in 1985 in Scotland, anA. baumannii strain that produced a plasmid-mediated ...
Background & objective: Multidrug-resistant Acinetobacter baumannii (MDR-AB) is an important nosocomial pathogen which is associated with significant morbidity and mortality, particularly in high-risk populations. Aminoglycoside-modifying enzymes (AMEs) and 16S ribosomal RNA (16S rRNA) methylation are two important mechanisms of resistance to aminoglycosides. The aim of this study was to determine the prevalence of 16S rRNA methylase (armA, rmtA, rmtB, rmtC, and rmtD), and the AME genes [aac(6′)-Ib, aac(3)-I, ant(3′′)-I, aph(3′)-I and aac(6)-Id], among clinical isolates of A. baumannii in Tehran, Iran. Methods: Between November 2015 to July 2016, a total of 110 clinical strains of A. baumannii were isolated from patients in two teaching hospitals in Tehran, Iran. Antimicrobial susceptibility testing was performed according to Clinical and Laboratory Standards Institute guidelines. The presence of genes encoding the AMEs and16S rRNA methylases responsible for resis-tance was investigated by
Molecular characterization and antimicrobial susceptibility of Acinetobacter baumannii isolates obtained from two hospital outbreaks in Los Angeles County, California, USA
This unit describes basic protocols for infecting mice through intranasal and intraperitoneal routes with Acinetobacter baumannii to induce associated pneumonia and sepsis, the two most common manifestations of clinical infections with this pathogen
Acinetobacter baumannii is an emerging nosocomial pathogen, responsible for infection outbreaks worldwide. The pathogenicity of this bacterium is mainly due to its multidrug-resistance and ability to form biofilm on abiotic surfaces, which facilitate long-term persistence in the hospital setting. Given the crucial role of iron in A. baumannii nutrition and pathogenicity, iron metabolism has been considered as a possible target for chelation-based antibacterial chemotherapy. In this study, we investigated the effect of iron restriction on A. baumannii growth and biofilm formation using different iron chelators and culture conditions. We report substantial inter-strain variability and growth medium-dependence for biofilm formation by A. baumannii isolates from veterinary and clinical sources. Neither planktonic nor biofilm growth of A. baumannii was affected by exogenous chelators. Biofilm formation was either stimulated by iron or not responsive to iron in the majority of isolates tested, ...
Acinetobacter baumannii outer membrane protein A targets the nucleus and induces cytotoxicity.: Acinetobacter baumannii is an emerging opportunistic pathogen re
Title:Prevalence of Metallo-β-Lactamases in Acinetobacter Baumannii in Iran: A Review and Meta-Analysis. VOLUME: 19 ISSUE: 4. Author(s):Bashir Mohammadpour*, Samaneh Rouhi, Masoud Moradi, Rashid Ramazanzadeh, Ebrahim Saniyi, Sairan Zandi and Himen Salimizand. Affiliation:Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Lung Diseases and Allergy Research Center, Kurdistan University of Medical Sciences, Sanandaj, Vice Chancellor for Research and Technology, Kurdistan University of Medical Sciences, Sanandaj, Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Watershed Management, Gorgan University of Agricultural Science and Natural Resource, Gorgan, Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Liver and Digestive Research Center, Kurdistan University of Medical Sciences, Sanandaj. Keywords:Metallo-β-Lactamases, Acinetobacter baumannii, Iran, ...
Since their identification in bacteria 30 years ago, MITEs have been reported in a multitude of species, displaying significant diversity in their nucleotide sequence and functional properties (44). In this study, a novel MITE was identified in environmental and clinical isolates of Acinetobacter species, including A. baumannii, one of the leading bacterial organisms threatening human health (2). This novel element lacked any CDS that could produce a functional transposase, inferring that like other MITEs, MITEAba12 is activated in trans. Given the high similarity between the TIR sequences of MITEAba12 and those of ISAba12 (Fig. 2D), we propose the transposase from ISAba12 elements were responsible for MITEAba12 mobilization in the A. baumannii ΔygiW strain. Whether ISAba12, or another IS with a TIR similar to that of MITEAba12 (Table 2), can mobilize MITEAba12 will need to be experimentally examined.. With the addition of MITEAba12, the list of nonautonomous elements reported in Acinetobacter ...
Management of multidrug-resistant organisms in healthcare settings, 2006. 2007. Kluytmans-Vandenbergh, MF, Kluytmans, JA, Voss, A. Dutch guideline for preventing nosocomial transmission of highly resistant microorganisms (HRMO). Infection. vol. 33. 2005. pp. 309-13. Peleg, AY, Seifert, H, Paterson, DL. Acinetobacter baumannii: emergence of a successful pathogen. Clin Microbiol Rev. vol. 21. 2008. pp. 538-82. Dijkshoorn, L, Nemec, A, Seifert, H. An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii. Nat Rev Microbiol. vol. 5. 2007. pp. 939-51. Nemec, A, Krízová, L, Maixnerová, M, Diancourt, L, van der Reijden, TJ, Brisse, S, van den Broek, P, Dijkshoorn, L. Emergence of carbapenem resistance in Acinetobacter baumannii in the Czech Republic is associated with the spread of multidrug-resistant strains of European clone II. J Antimicrob Chemother. vol. 62. 2008. pp. 484-9. Higgins, PG, Dammhayn, C, Hackel, M, Seifert, H. Global spread of carbapenem-resistant ...
The present study aimed to perform a deep phenotypic and genotypic analysis of 15 clinical carbapenem-resistant Acinetobacter baumannii (CRAb) strains isolated in Madagascar between 2008 and 2016 from diverse sources. CRAb isolates collected from the Clinical Biology Centre of the Institut Pasteur of Madagascar, from the neonatal unit of Antananarivo military hospital, and from intensive care units of Mahajanga Androva and Antananarivo Joseph Ravoahangy Andrianavalona (HJRA) hospitals were subjected to susceptibility testing. Whole-genome sequencing allowed us to assess the presence of antibiotic-resistance determinants, insertion sequences, integrons, genomic islands and potential virulence factors in all strains. The structure of the carO porin gene and deduced protein (CarO) were also assessed in CRAb isolates. All isolates were found to be multidrug-resistant strains. Antibiotic-resistance genes against six classes of antimicrobial agents were described. The four carbapenem-resistance genes: blaOXA
Acinetobacter baumannii is an opportunistic pathogen causing various nosocomial infections. The spread of multidrug-resistant A. baumannii is a major public health problem. The aim of this study was to investigate the molecular epidemiology and the genetic support of multidrug-resistant A. baumannii isolates collected from Saint-Georges Hospital in Lebanon. Between January and August 2016, 31 A. baumannii isolates were collected from sputum samples of patients infected with ventilator-associated pneumonia (VAP) and treated with colistin-carbapenem combination therapy. Antibiotic susceptibility testing was performed using the disk diffusion method. Carbapenemases, extended spectrum β-lactamases encoding genes and mcr-1/2 genes were investigated by RT-PCR and standard PCR. The epidemiological relatedness of the strains was studied using MLST analysis. Most of the isolates exhibited multidrug-resistant phenotypes. All the isolates were carbapenem-resistant and among them, 30 carried the class D
Journal Article: Small-Molecule Transport by CarO, an Abundant Eight-Stranded beta-Barrel Outer Membrane Protein from Acinetobacter Baumannii ...
Objective: Carbapenem-resistant Acinetobacter baumannii (CR-AB) is an emerging cause of nosocomial infections worldwide. Combination therapy may be the only viable option until new antibiotics become available. The objective of this study is to identify potential antimicrobial combinations against CR-AB isolated from our local hospitals. Methods: AB isolates from all public hospitals in Singapore were systematically collected between 2006 and 2007. MICs were determined according to CLSI guidelines. All CR-AB isolates were genotyped using a PCR-based method. Clonal relationship was elucidated. Time-kill studies (TKS) were conducted with polymyxin B, rifampicin and tigecycline alone and in combination using clinically relevant (achievable) unbound concentrations. Results: 31 CR AB isolates were identified. They are multidrug-resistant, but are susceptible to polymyxin B. From clonal typing, 8 clonal groups were identified and 11 isolates exhibited clonal diversity. In single TKS, polymyxin B, ...
OmpA Binding Mediates the Effect of Antimicrobial Peptide LL-37 on Acinetobacter baumannii. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Background Kentucky Department for Public Health (KDPH) notified of Acinetobacter outbreak in September, 2010 Kentucky Department for Public Health (KDPH) notified of Acinetobacter outbreak in September, cases initially reported 66 cases initially reported Assistance requested from KDPH Assistance requested from KDPH New Infection Preventionist New Infection Preventionist Reported gaps in infection control process Reported gaps in infection control process Hand hygiene Hand hygiene Lacked comprehensive environmental cleaning protocols Lacked comprehensive environmental cleaning protocols 3
Acinetobacter baumannii has been associated with high morbidity and mortality rates, even in pediatric patients. Therapeutic options are limited, especially when the strain is multidrug resistant. Clinical and microbiological analyses of 4 cases of systemic infections caused by multi drug resistant A. baumannii treated with colistin/vancomycin combination at a Pediatric Intensive Care Unit were performed in order to explore the potential synergistic activity of colistin plus vancomycin. All the patients were treated with colistin, meropenem and vancomycin. Four severe infections due to MDR A. baumannii were observed. All patients treated with colistin/vancomycin combination had a positive outcome with no infection relapses. Most importantly, no significant adverse events related to the simultaneous administration of COL plus VAN were observed. In our in-vitro experiments, the synergistic effect of the combination COL plus VAN showed an early bactericidal activity even at VAN concentration of 16 mg/L,
Clinical features and outcomes of Acinetobacter species and Pseudomonas aeruginosa. Presented at the 46th Interscience infections due buy Xanax tablets online UK multidrug resistant baumannii Ab bloodstream infections BSI. Antimicrob Agents Chemother 200751376 378 391 Fournier PE. Clin Infect Dis 1996221026 1032 Carey RB, Banerjee SN. baumannii correlated with an increased 1999 Marmara earthquake. Surveillance cultures and duration of carriage of multidrug resistant Acinetobacter. Seasonal variation of Acinetobacter infections Clin Microbiol Rev 200619257. J Clin Microbiol 2006443623 3627 of resistance to tigecycline has. Crit Care Med 2005331136 1140 of broad spectrum antibiotics. References Schreckenberger PC, Daneshvar with multidrug resistant Pseudomonas aeruginosa. Presented at the 46th Interscience outbreaks of acinetobacter infections, most even more limited. PLoS Genet 20062e7 e7 of Acinetobacter spp. Source Information From Medical for nonsusceptibility in Acinetobacter baumannii. 79 An ...
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TY - JOUR. T1 - Acinetobacter species as model microorganisms in environmental microbiology. T2 - current state and perspectives. AU - Jung, Jaejoon. AU - Park, Woojun. PY - 2015/3/1. Y1 - 2015/3/1. N2 - Acinetobacter occupies an important position in nature because of its ubiquitous presence in diverse environments such as soils, fresh water, oceans, sediments, and contaminated sites. Versatile metabolic characteristics allow species of this genus to catabolize a wide range of natural compounds, implying active participation in the nutrient cycle in the ecosystem. On the other hand, multi-drug-resistant Acinetobacter baumannii causing nosocomial infections with high mortality has been raising serious concerns in medicine. Due to the ecological and clinical importance of the genus, Acinetobacter was proposed as a model microorganism for environmental microbiological studies, pathogenicity tests, and industrial production of chemicals. For these reasons, Acinetobacter has attracted significant ...
outbreaks of carbapenem-resistant A. baumannii infection occurred in a hospital in New York City. Subsequently, numerous other hospitals in the United States and South America have had outbreaks of carbapenem-resistant A. baumannii. The incidence of infections with A. baumannii among military personnel from the United States and Canada has increased since 2002; 102 patients had bloodstream infections at facilities treating U.S. military personnel injured in Iraq or Afghanistan from January 1, 2002, through August 31, 2004. An epidemiologic investigation revealed that A. baumannii could be grown from environmental sites in field hospitals and that the environmental strains were closely related genotypically to clinical isolates. A. baumannii strains from injured military personnel from the United States and the United Kingdom were also genotypically related; this finding provided further evidence that A. baumannii was being acquired in field hospitals.. ...
War wound infection and osteomyelitis caused by multidrug-resistant (MDR) Acinetobacter species have been prevalent during the 2003-2005 military operations in Iraq. Twenty-three soldiers wounded in Iraq and subsequently admitted to our facility from March 2003 to May 2004 had wound cultures positive for Acinetobacter calcoaceticus-baumannii complex. Eighteen had osteomyelitis, 2 burn infection, and 3 deep wound infection. Primary therapy for these infections was directed antimicrobial agents for an average of 6 weeks. All soldiers initially improved, regardless of the specific type of therapy. Patients were followed up to 23 months after completing therapy, and none had recurrent infection with Acinetobacter species. Despite the drug resistance that infecting organisms demonstrated in this series, a regimen of carefully selected extended antimicrobial-drug therapy appears effective for osteomyelitis caused by MDR Acinetobacter spp.
REQUIMTE- Microbiology Research Group Goals Goals Epidemiology study of antibiotic resistant bacteria in order to implement containment measures, methodologies for their detection, and design of new compounds Hospitals Food and animal production environment Humans community Environment VRE, Enterobacteriaceae- PL/TEM-52; PL/CTX-M-15, Pseudomonas-VIM Enterococcus gentaR, Tn1546 PL/CTX-M-14; PL/CTX-M-15 (Enterobacteriaceae) VRE-CC17 Acinetobacter OXA-23 Salmonella-intI1 OXA-30, sul3 Enterobacteriaceae- PL/TEM-52 Salmonella-intI1MDR, Enterobacteriaceae- PL/TEM-52 Strategies Characterization of bacteria, genes and mobile genetic elements from different ecological niches AAC :1001 JAC (In press) AAC :1545 CMI :1131 JAC :297 CMI :1047 JAC :1139 EID :1985 AAC :836 AAC : AAC :451 JAC :1370 AAC :3613 AEM :3743 AAC :2140 AEM :3364 CMI :755 AAC :3220 Main Achievements (1) Emergence and International dissemination of MDR strains: VRE, ESBL-producing Enterobacteriaceae, Carbapenemase-producing Pseudomonas and
Infection with antibiotic-resistant Acinetobacter spp. is an increasing problem in critical care environments worldwide. Acinetobacter spp. are known to produce an insulin-cleaving protease. We hypothesized that infection with Acinetobacter spp. was associated with the acquisition of glucose intolerance in burn patients. Data were collected prospectively on all 473 patients admitted to the Burns Centre between January 2002 and March 2003. A total of 3.4% of patients acquired glucose intolerance during admission. Patients with Acinetobacter spp. infection were 9.8 times more likely to develop glucose intolerance than those without the infection (P | .0001). The association persisted after controlling for TBSA (P | .001). In patients with deep Acinetobacter spp. infection, 47% had glucose intolerance, compared with 12% in those with infection of the burn only (P = .03). In patients with pre-existing diabetes mellitus, 27% developed Acinetobacter spp. infection compared with only 8.5% of patients without
Introduction: Ventilator-associated pneumonia (VAP) caused by multidrug-resistant Acinetobacter baumannii (MDR-AB) is common in hospitals and impacts patient survival. We determined the incidence of MDR-AB VAP in critical care units and examined the predictors of 14-day mortality in these patients. Methodology: A retrospective case series study was conducted at a tertiary referral teaching hospital in north Jordan. A list of patients with a positive culture of A. baumannii between January 2007 and June 2013 was retrieved using computerized hospital databases. Medical records of all these patients were reviewed, and cases of VAP infected with MDR-AB were identified. Predictors of 14-day mortality were determined using multivariable logistic regression adjusted for possible confounders. Results: Out of 121 A. baumannii-VAP cases, 119 (98.3%) were caused by MDR-AB. The incidence rate of MDR-AB VAP was 1.59 cases per 100 critical care unit admissions. The mortality of A. baumannii-VAP cases in critical
title: In Vitro Interactions of Antibiotic Combinations of Colistin, Tigecycline, and Doripenem Against Extensively Drug-Resistant and Multidrug-Resistant Acinetobacter baumannii, doi: 10.3343/alm.2016.36.2.124, category: Article
Sayer refers to an example from the Korean Journal of Physiology and Pharmacology titled, Black raspberry root polyphenols exhibit antibacterial activity against drug resistant bacteria. He notes that the root-not the berry-of the black raspberry plant contains polyphenols which are lethal to methicillin-resistant Staphylococcus aureus (MRSA),carbapenem-resistant Acinetobacter baumannii (CRAB), and Bacillus anthracis (Anthrax). The roots contain these polyphenols because they dig into soil, a potentially hostile environment, while the berries dont have it because they serve as food for animals that help spread the black raspberry seed.. The fauna and flora of Gaia-our earth, our home-have learned over eons how to survive. Yet medicine presumes to know better. Antibiotics were first used in wars because the most common means of death in war isnt wounds, but the infections that develop from them. For this purpose, antibiotics worked brilliantly. But it must be noted that wholesale war is a ...
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Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. ...
The pentose phosphate pathway is a process of glucose turnover that produces NADPH as reducing equivalents and pentoses as essential parts of nucleotides. There are two different phases in the pathway. One is irreversible oxidative phase in which glucose-6P is converted to ribulose-5P by oxidative decarboxylation, and NADPH is generated [MD:M00006]. The other is reversible non-oxidative phase in which phosphorylated sugars are interconverted to generate xylulose-5P, ribulose-5P, and ribose-5P [MD:M00007]. Phosphoribosyl pyrophosphate (PRPP) formed from ribose-5P [MD:M00005] is an activated compound used in the biosynthesis of histidine and purine/pyrimidine nucleotides. This pathway map also shows the Entner-Doudoroff pathway where 6-P-gluconate is dehydrated and then cleaved into pyruvate and glyceraldehyde-3P [MD:M00008 ...
tRNA-specific adenosine deaminase; Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2); Belongs to the cytidine and deoxycytidylate deaminase family (158 aa ...
Cytosine/purine/uracil/thiamine/allantoin permease family protein; Derived by automated computational analysis using gene prediction method- Protein Homology; Belongs to the purine-cytosine permease (2.A.39) family (468 aa ...
Rayani, Arezoo and Ahanjan, Mohammad and Goli, Hamid Reza and Naderifard, Niloofar and zamanzdeah, Maryam (2020) Comparing the Effect of Probiotic and Non-probiotic Yogurt Drinks on Two Common Oral Microorganisms: An In Vitro Study. Journal of Mazandaran University of Medical Sciences, 30 (185). pp. 33-40. Ranjbar, Termeh and Hashemi, zahra and Sadeghian, Fereshteh and Goli, Hamid Reza and Ahanjan, Mohammad and Ebrahimzadeh, Mohammad Ali (2020) Green Synthesis of Silver Nanoparticles with Allium paradoxum Extract and Evaluation of their Antibacterial Activities. Journal of Mazandaran University of Medical Sciences, 29 (182). pp. 1-11. NorouziBazgir, Zahra and Ahanjan, Mohammad and MohammadBagher, Hashemi Soteh and Goli, Hamid Rez (2019) Correction to: Prevalence of IMP and SPM Genes in Clinical Isolates of Carbapenem Resistant Acinetobacter baumannii in Educational Hospitals of Sari, Iran. Journal of Mazandaran University of Medical Sciences, 29 (176). pp. 227-228. NorouziBazgir, Zahra and ...
Bacterial infections due to Acinetobacter species are typically encountered in health care settings and can be particularly difficult to treat due to the propensity of the organism to incorporate multiple antibiotic resistance mechanisms. Hospital outbreaks of multidrug-resistant Acinetobacter infections have been reported. A wide range of infections are possible with this organism, including bloodstream infections, pneumonia (occasionally even community-acquired pneumonia), urinary tract infections, and wound infections (wound infection in soldiers after traumatic injury have been reported ...
TY - JOUR. T1 - A Novel Phenotypic Method To Screen for Plasmid-Mediated Colistin Resistance among Enterobacteriales. AU - Bell, Drew T.. AU - Bergman, Yehudit. AU - Kazmi, Abida Q.. AU - Lewis, Shawna. AU - Tamma, Pranita. AU - Simner, Patricia. PY - 2019/5/1. Y1 - 2019/5/1. N2 - Plasmid-mediated colistin resistance (PMCR), a consequence of the mcr genes, is a significant public health concern given its potential to easily spread among clinical pathogens. Recently, it was discovered that MCR enzymes require zinc for activity. Thus, we modified the colistin broth-disk elution (CBDE) test to screen for plasmid-mediated colistin resistance (PMCR) genes based on any reduction of colistin MIC in the presence of EDTA. Eighty-five isolates of the order Enterobacteriales (12 mcr positive) were tested by CBDE ± EDTA. The sensitivity and specificity of the EDTA-CBDE method to detect PMCR compared to the molecular genotype results were 100% and 95.8%, respectively. Isolates positive by the EDTA-CBDE test ...
TY - JOUR. T1 - Comparative genomic analysis of Acinetobacter oleivorans DR1 To determine strain-specific genomic regions and gentisate biodegradation. AU - Jung, Jaejoon. AU - Madsen, Eugene L.. AU - Jeon, Che Ok. AU - Park, Woojun. PY - 2011/10. Y1 - 2011/10. N2 - The comparative genomics of Acinetobacter oleivorans DR1 assayed with A. baylyi ADP1, A. calcoaceticus PHEA-2, and A. baumannii ATCC 17978 revealed that the incorporation of phage-related genomic regions and the absence of transposable elements have contributed to the large size (4.15 Mb) of the DR1 genome. A horizontally transferred genomic region and a higher proportion of transcriptional regulator- and signal peptide-coding genes were identified as characteristics of the DR1 genome. Incomplete glucose metabolism, metabolic pathways of aromatic compounds, biofilm formation, antibiotics and metal resistance, and natural competence genes were conserved in four compared genomes. Interestingly, only strain DR1 possesses gentisate ...
Tigecycline is a last-resort antibiotic that is used to treat severe infections caused by extensively drug-resistant bacteria. tet(X) has been shown to encode a flavin-dependent monooxygenase that modifies tigecycline1,2. Here, we report two unique mobile tigecycline-resistance genes, tet(X3) and tet(X4), in numerous Enterobacteriaceae and Acinetobacter that were isolated from animals, meat for consumption and humans. Tet(X3) and Tet(X4) inactivate all tetracyclines, including tigecycline and the newly FDA-approved eravacycline and omadacycline. Both tet(X3) and tet(X4) increase (by 64-128-fold) the tigecycline minimal inhibitory concentration values for Escherichia coli, Klebsiella pneumoniae and Acinetobacter baumannii. In addition, both Tet(X3) (A. baumannii) and Tet(X4) (E. coli) significantly compromise tigecycline in in vivo infection models. Both tet(X3) and tet(X4) are adjacent to insertion sequence ISVsa3 on their respective conjugative plasmids and confer a mild fitness cost (relative ...
Acrônimos com ACINETOBACTER. As definições de siglas Acinetobacter. As definições de acrónimo Acinetobacter. Sigla Acinetobacter significa para. Além de encontrar siglas. Encontre o que significam as siglas!
Newly published findings by a team of researchers in China describe the discovery of a plasmid-mediated mechanism in E. coli isolated from pigs that confers resistance to colistin - widely regarded as an antibiotic of last resort for treating drug resistant infections.. The researchers discovered the mechanism during a surveillance project looking at antibiotic resistance in E. coli from food animals when they found a large increase in the prevalence of colistin resistance in isolates. Further investigation revealed that a strain isolated from a pig, SHP45, possessed colistin resistance that could be transferred horizontally to another strain.. These findings led to the identification of a gene, mcr-1, conferring resistance to colistin and located on a plasmid within the bacterial cell. This is the first time that a resistance mechanism against polymyxin antibiotics like colistin has been found that is plasmid mediated and can be transferred horizontally to other bacteria.. Further studies ...
Carbapenems have traditionally been used to treat infections caused by Gram-negative bacteria resistant to many other agents, as they usually escaped the hydrolytic activity of most clinically relevant β-lactamases and are little affected (in terms of absolute MIC changes) by permeability and active efflux mechanisms. For years, resistance to these agents was mainly confined to non-fermentative Gram-negative bacteria resistant to ertapenem, and a few species (most notably Stenotrophomonas maltophilia) with intrinsic resistance to all carbapenems, because of a chromosomal encoded carbapenemase.. In 1991, a transferable carbapenemase was reported in Pseudomonas aeruginosa (strain GN17203).1 Shortly after, OXA-23 (ARI-1)2 in Acinetobacter baumannii and KPC-1 in Klebsiella pneumoniae3 were also described. In the following years, organisms producing plasmid-mediated carbapenemases have rapidly spread worldwide. A pooled analysis of nine studies comparing mortality in infections caused by ...
Aim: To determine the prevalence of New Delhi metallo-β-lactamase (NDM)-producing Gram-negative pathogens isolated from childrens samples. Materials & methods: Carbapenem-resistant clinical isolates (n = 117) were confirmed by VITEK® 2 compact system, matrix-assisted laser desorption ionization-time of flight and multilocus sequence typing. MIC (μg/ml) of various antibiotics was determined by VITEK 2 compact system. Molecular characterization of the isolates was performed by PCR, DNA sequencing, PFGE and DNA hybridization. Results: Out of 117 carbapenemase producers, 37 (31.6%) and 29 (24.7%) were Klebsiella pneumoniae and Acinetobacter baumannii, respectively. 72 (61.5%) isolates were NDM positive and among these 60, 9 and 3 were NDM-1, -5 and -7, respectively. Majority of the NDM-producing K. pneumoniae belonged to ST11 and ST273 while most of the E. coli belonged to ST405 and ST101. blaNDM were mainly located on 150kb plasmids. MIC displayed high resistance against β-lactams drugs ...
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海词词典,最权威的学习词典,专业出版acinetobacter baummanii是什么意思,acinetobacter baummanii的用法,acinetobacter baummanii翻译和读音等详细讲解。海词词典:学习变容易,记忆很深刻。
Abstract: Chlorhexidine is widely used as an antiseptic or disinfectant in both hospital and community settings. A number of bacterial species display resistance to this membrane-active biocide. We examined the transcriptomic response of a representative nosocomial human pathogen, Acinetobacter baumannii, to chlorhexidine to identify the primary chlorhexidine resistance elements. The most highly up-regulated genes encoded components of a major multidrug efflux system, AdeAB. The next most highly overexpressed gene under chlorhexidine stress was annotated as encoding a hypothetical protein, named here as AceI. Orthologs of the aceI gene are conserved within the genomes of a broad range of proteobacterial species. Expression of aceI or its orthologs from several other γ- or β-proteobacterial species in Escherichia coli resulted in significant increases in resistance to chlorhexidine. Additionally, disruption of the aceI ortholog in Acinetobacter baylyi rendered it more susceptible to ...
Abstract: Chlorhexidine is widely used as an antiseptic or disinfectant in both hospital and community settings. A number of bacterial species display resistance to this membrane-active biocide. We examined the transcriptomic response of a representative nosocomial human pathogen, Acinetobacter baumannii, to chlorhexidine to identify the primary chlorhexidine resistance elements. The most highly up-regulated genes encoded components of a major multidrug efflux system, AdeAB. The next most highly overexpressed gene under chlorhexidine stress was annotated as encoding a hypothetical protein, named here as AceI. Orthologs of the aceI gene are conserved within the genomes of a broad range of proteobacterial species. Expression of aceI or its orthologs from several other γ- or β-proteobacterial species in Escherichia coli resulted in significant increases in resistance to chlorhexidine. Additionally, disruption of the aceI ortholog in Acinetobacter baylyi rendered it more susceptible to ...
The use of imipenem antibiotics to treat A. baumannii infections as the premier agents continued well in the 1990s. Despite the growing incidence of resistance, the level of imipenem use remained relatively high. The persistence of its use in clinical settings can be explained by its superior activity against A.baumannii compared to other antimicrobial agents, even with its growing resistance[63]. As the resistance of imipenem continued to grow, another bacteriostatic carbapenem began to be used in greater frequency-meropenem. In addition to be a broad-spectrum agent against gram-negative bacteria, meropenem is also less likely to cause seizures than imipenem[64]. Besides the continued the use of carbapenems, the combination of anti-A.baumannii agents was a major therapeutic strategy employed in the 1990s. The main purpose of these pairings was to put together drugs that would have similar effects as a monotherapy drug of the same class. This usually involved pairing a drug with high resistance ...
From the Staphylococcus aureus, 40% strains presented resistance to oxacillin (MRSA), 96.9% were resistant to penicillin G, 75.8% to erythromycin and 63.3% to ciproflxacin and levoflxacin. S. aureus strains resistant to linezolid (3.9%), daptomycin (4.7%) and vancomycin (2.3%) wer e also found.. When analyzing the susceptibility profile for Klebsiella pneumoniae, it was possible to observe that this strain presented the highest resistance rates to the following antimicrobials: aztreonam, cefepime and cefotaxime (75.2%) and ertapenem (69.7%); and the lowest rate of resistance was 2.6% to amikacin.. Acinetobacter baumannii presented the most worrying susceptibility profile, presenting a resistance frequency of 100% to imipenem; 91.1% to ciprofloxacin, ceftazidime and cefotaxime; 88.8% to cefepime; 86% to meropenem and 82.2% to levofloxacin.. The highest resistance rates to Pseudomonas aeruginosa were 54.3% to ciprofloxacin, 53.1% to levofloxacin and imipenem, and 47% to norfloxacin. While that to ...
The emergence of carbapenemase producing bacteria, especially New Delhi metallo-β-lactamase (NDM-1) and its variants, worldwide, has raised amajor public health concern. NDM-1 hydrolyzes a wide range of β-lactam antibiotics, including carbapenems, which are the last resort of antibiotics for the treatment of infections caused by resistant strain of bacteria. In this review, we have discussed bla NDM-1variants, its genetic analysis including type of specific mutation, origin of country and spread among several type of bacterial species. Wide members of enterobacteriaceae, most commonly Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, and gram-negative non-fermenters Pseudomonas spp. and Acinetobacter baumannii were found to carry these markers. Moreover, at least seventeen variants of bla NDM-type gene differing into one or two residues of amino acids at distinct positions have been reported so far among different species of bacteria
Development of a Multilocus Sequence Typing Scheme for Characterization of Clinical Isolates of Acinetobacter baumannii: In this study a multilocus sequence typ
About ContraFect: ContraFect is a biotechnology company focused on the discovery and development of DLAs, including lysins and amurin peptides, as new medical modalities for the treatment of life-threatening, antibiotic-resistant infections. An estimated 700,000 deaths worldwide each year are attributed to antimicrobial-resistant infections. We intend to address life threatening infections using our therapeutic product candidates from our platform of DLAs, which include lysins and amurin peptides. Lysins are a new class of DLAs which are recombinantly produced antimicrobial proteins with a novel mechanism of action associated with the rapid killing of target bacteria, eradication of biofilms and synergy with conventional antibiotics. Amurin peptides are a novel class of DLAs which exhibit broad-spectrum activity against a wide range of antibiotic-resistant Gram-negative pathogens, including P. aeruginosa, Acinetobacter baumannii, and Enterobacter species. We believe that the properties of our ...
Background: Metallo-β-lactamases (MBLs) are bacterial enzymes that hydrolyze carbapems. MBL-producing gram-negative bacilli have been emerging worldwide. In this study, different MBLs were identified in various lung diseases in the japanese clinical hospitals.. Methods: From Jan. 2009 to Dec. 2010, 1618 GNB strains were submitted to the laboratory of 6 general hospitals in Aichi prefecture. Strains demonstrating a high level ceftazidime resistance (MIC, ,128 mg/ml) were subjected to a screening test for MBL production by using disks containing an MBL inhibitor, sodium mercaptoacetic acid (SMA). PCR and sequencing analyses were performed to confirm the types of MBLs and integrases using primers specific for each gene.. Results: Fifty-three strains (34 Pseudomonas aeruginosa, 11 P. putida and 8 Acinetobacter baumannii) were isolated from elderly patients in various wards. These strains were isolated from various respiratory specimens (sputum, pus). By PCR analyses, 35 IMP-1 producers (26 P. ...
METHODS: In silico translation and digestion were performed on 14-25 whole genomes representing strains of Acinetobacter baumannii, Moraxella catarrhalis, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Klebsiella pneumoniae. Peptides constituting theoretical core peptidomes in each were identified. Rapid tryptic digestion was performed; peptides were analyzed by LC-MS/MS and compared with the theoretical core peptidomes. High-confidence core peptides (false discovery rate ,1%) were identified and analyzed with the lowest common ancestor search to yield potential species-specific peptide markers. The species specificity of each peptide was verified with protein BLAST. Further, 1 or 2 pathogens were serially diluted into pooled inflamed BAL, and a targeted LC-MS/MS assay was used to detect 25 peptides simultaneously. ...
Infectious diseases represent an ever-changing field of practice. Virtually each week brings new information, some with the potential to adjust or reinvent the current clinical practice. It is our task as clinicians to browse through field literature, through reports of randomized controlled trials, excerpts of conference proceedings, and to choose relevant scoops with high applicability in patient management.. This supplement brings together four articles relevant to four different areas of medical practice, from HIV infection to Gram-negative bacilli such as Acinetobacter baumannii and Pseudomonas aeruginosa, and interdisciplinary studies on infectious acute maxillary sinusitis.. Non-infectious comorbidities are of utmost importance in the management of HIV infection, as occurrence of kidney impairment, or fractures following decrease in bone mineral density, can significantly impact the quality of life and/or even life expectancy. The study presented at the 11th Edition of the Scientific Days ...
NIAID recently announced a research funding opportunity to develop and/or produce diagnostics to quickly detect the key bacteria responsible for antibacterial-resistant infections in hospital settings. The request for applications (RFA) will support diagnostics research related to one or more of the following types of bacteria: Klebsiella pneumonia, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter species, and extra-intestinal pathogenic Eschericihia coli. NIAID expects to fund 10-15 awards for a total of up to $12 million in 2015, and the maximum length of each award is 5 years ...
"Medscape abstract on Acinetobacter baumannii: Acinetobacter baumannii: An Emerging Multidrug-resistant Threat". membership only ... Acinetobacter baumannii[editar , editar a fonte]. En 2004, os Centros para o Control de Enfermidades e Prevención (CDC) de EUA ... Acinetobacter resistente a multidrogas[editar , editar a fonte]. Acinetobacter é unha baceria gramnegativa que causa pneumonía ... "Acinetobacter baumannii infections among patients at military medical facilities treating injured U.S. service members, 2002- ...
Acinetobacter infections Acinetobacter baumannii Actinomycosis Actinomyces israelii, Actinomyces gerencseriae and ...
"Deciphering the Multifactorial Nature of Acinetobacter baumannii Pathogenicity". PLOS One. 6 (8): e22674. Bibcode:2011PLoSO... ...
Isolation and characterization of an autoinducer synthase from Acinetobacter baumannii. J Bacteriol. 2008;190(9):3386-3392. doi ... Acinetobacter baumannii abaR (activates operon for production of surfactant-like lipopeptide acinetin-505) 3. Autonomous ... Characterization of RelA in Acinetobacter baumannii [published online ahead of print, 2020 Mar 30]. J Bacteriol. 2020;JB.00045- ...
It also demonstrates potent in vitro activity versus carbapenem-resistant Acinetobacter baumannii. Plazomicin was found to be ... "Can Plazomicin Alone or in Combination Be a Therapeutic Option against Carbapenem-Resistant Acinetobacter baumannii?" (PDF). ...
"Medscape abstract on Acinetobacter baumannii: Acinetobacter baumannii: An Emerging Multidrug-resistant Threat". membership only ... Centers for Disease Control Prevention (CDC). (2004). "Acinetobacter baumannii infections among patients at military medical ... reported an increasing number of Acinetobacter baumannii bloodstream infections in patients at military medical facilities in ... Acinetobacter is a gram-negative bacteria that causes pneumonia or bloodstream infections in critically ill patients. Multidrug ...
"PheWAS uncovers a pathological role of coagulation Factor X during Acinetobacter baumannii infection". Infection and Immunity. ...
... resistant Acinetobacter baumannii". Respirology Case Reports. 8 (8): e00662. doi:10.1002/rcr2.662. PMC 7507560. PMID 32999723. ... Acinetobacter baumannii, Streptococcus pyogenes, Stenotrophomonas maltophilia, anaerobes like Fusobacterium nucleatum and ...
"Genetic basis of high level aminoglycoside resistance in Acinetobacter baumannii from Beijing, China". Acta Pharmaceutica ...
Use of colistin to treat Acinetobacter baumannii infections has led to the development of resistant bacterial strains. which ... Colistimethate sodium has also been given intrathecally and intraventricularly in Acinetobacter baumannii and Pseudomonas ... Towner K J (2008). "Molecular Basis of Antibiotic Resistance in Acinetobacter spp.". Acinetobacter Molecular Biology. Caister ... "In-vitro activity of the combination of colistin and rifampicin against multidrug-resistant strains of Acinetobacter baumannii ...
Expression of chemosensory protein (CSP) structures in Pediculus humanis corporis and Acinetobacter baumannii. SOJ Microbiol ... B-CSP/Acinetobacter baumannii, Macrococcus caseolyticus, Kitasatospora griseola, Escherichia coli (Liu et al., 2019) [24]. The ... They have been reported from bacterial species such as coccobacillus Acinetobacter baumannii, Macrococcus/Staphylococcus ...
"Successful treatment of multidrug-resistant Acinetobacter baumannii ventriculitis with intravenous and intraventricular ...
... resistant Acinetobacter baumannii". Respirology Case Reports. 8 (8): e00662. doi:10.1002/rcr2.662. PMC 7507560. PMID 32999723. ...
"Overexpression of resistance-nodulation-cell division pump AdeFGH confers multidrug resistance in Acinetobacter baumannii". ...
"Isolation and characterization of antimicrobial compounds in plant extracts against multidrug-resistant Acinetobacter baumannii ...
In addition, VCC has been applied to Salmonella enterica serovar Typhimurium and Acinetobacter baumannii. The initial virtual ... HD5 variants with one disulfide bond or no disulfide bonds exhibited greatly decreased VCC activity against A. baumannii, ... derivative of human defensin 5 with potent and efficient activity against multidrug-resistant Acinetobacter baumannii". ... baumannii. These studies have been expanded to include additional beta defensins, theta defensins, and the human cathelicidin ...
In Vitro Efficacy of Doripenem against Pseudomonas Aeruginosa and Acinetobacter Baumannii by E-test. Journal of the College of ...
Acinetobacter baumannii. "https://eu.wikipedia.org/w/index.php?title=Kategoria:1986._urtean_deskribatutako_taxoiak&oldid= ...
2005). "Ethanol-induced virulence of Acinetobacter baumannii". American Society for Microbiology meeting. Volume 1 June 5 - ...
Acinetobacter baumannii Stenotrophomonas maltophilia Haemophilus influenzae Moraxella catarrhalis Neisseria gonorrhoeae ... "Activity of eravacycline against Enterobacteriaceae and Acinetobacter baumannii, including multidrug-resistant isolates, from ...
Effects of Group 1 versus Group 2 carbapenems on the susceptibility of Acinetobacter baumannii to carbapenems: a before and ... "Challenges in identifying new antimicrobial agents effective for treating infections with Acinetobacter baumannii and ... It lacks useful activity against the P. aeruginosa and Acinetobacter species, both of which are important causes of hospital- ... Antibiotics cross the outer membrane of Pseudomonas and Acinetobacter approximately 100 times more slowly than they cross the ...
In July 2010, a team in New Delhi reported a cluster of three cases of Acinetobacter baumannii bearing blaNDM-1 that were found ... "Coexistence of blaOXA-23 with blaNDM-1 and armA in clinical isolates of Acinetobacter baumannii from India". J Antimicrob ... The patient was also found to be carrying an Acinetobacter strain. The patient contracted the bacteria after another patient, ...
"A Plasmid-Borne blaOXA-58 Gene Confers Imipenem Resistance to Acinetobacter baumannii Isolates from a Lebanese Hospital". ...
2011). "Whole-genome comparison of two Acinetobacter baumannii isolates from a single patient, where resistance developed ... 2014). "Genomic epidemiology of a protracted hospital outbreak caused by multidrug-resistant Acinetobacter baumannii in ...
April 2007). "Control of an outbreak of pandrug-resistant Acinetobacter baumannii colonization and infection in a neonatal ... and Gram-negative Acinetobacter baumannii. While antibiotic drugs to treat diseases caused by methicillin-resistant ... Acinetobacter bacteria are evolving and becoming immune to antibiotics, so in many cases, polymyxin-type antibacterials need to ... aureus Methicillin resistant Staphylococcus aureus Candida albicans Pseudomonas aeruginosa Acinetobacter baumannii' ...
... and Acinetobacter baumannii". Int. J. Antimicrob. Agents. 34 (5): 395-401. doi:10.1016/j.ijantimicag.2009.06.021. PMID 19665876 ... aureus infection and a disease caused by drug-resistant Acinetobacter spp. A list of uses includes: Amoebic dysentery Anthrax ...
Acinetobacter baumannii is most common in hospitals, which has allowed for the development of resistance to all known ... Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. This group of Gram-positive and Gram-negative bacteria ... The Gram-negative aspects of the membrane surface of A. baumannii, including the efflux pump and outer membrane, affords it a ... The Gram-negative short-rod-shaped (coccobacillus) A. baumannii thrives in a number of unaccommodating environments due to its ...
Acinetobacter baumannii and Evaluation of Their Therapeutic Efficacy in Vivo". Frontiers in Microbiology. 9: 696. doi:10.3389/ ... In 2017 a patient with a pancreas compromised by MDR A. baumannii was put on several antibiotics, despite this the patient's ... Personalized Bacteriophage-Based Therapeutic Cocktails To Treat a Patient with a Disseminated Resistant Acinetobacter baumannii ... baumannii. Mice treated with the phage cocktail showed a 2.3-fold higher survival rate than those untreated in seven days post ...
Acinetobacter baumannii, Pseudomonas aeruginosa Multi-drug-resistant tuberculosis Overlapping with MDRGN, a group of Gram- ... Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter species). Various microorganisms have survived for thousands ... Personalized Bacteriophage-Based Therapeutic Cocktails To Treat a Patient with a Disseminated Resistant Acinetobacter baumannii ...
A study also showed essential oil from Z. cassumunar had anti-microbial activity against XDR Acinetobacter baumannii and ... Roxb against extensively drug-resistant Acinetobacter baumannii strains". Journal of Infection and Public Health. 10 (5): 586- ...
Acinetobacter baumannii. Xanthomonadaceae. *Stenotrophomonas maltophilia. Cardiobacteriaceae. *Cardiobacterium hominis *HACEK. ...
Acinetobacter baumannii. *Agrobacterium tumefaciens. *Alcaligenes eutrophus. B. *Bacillus anthracis. *Bacteroides. * ...
Acinetobacter baumannii. Xanthomonadaceae. *Stenotrophomonas maltophilia. Cardiobacteriaceae. *Cardiobacterium hominis *HACEK. ...
Acinetobacter baumannii. Xanthomonadaceae. *Stenotrophomonas maltophilia. Cardiobacteriaceae. *Cardiobacterium hominis *HACEK. ...
Acinetobacter baumannii, addition of antibiotic specific to gram-negative organism is recommended. For Methicillin-resistant ...
Pseudomonas aeruginosa (Pseudomonas infection) · Moraxella catarrhalis · Acinetobacter baumannii. Xanthomonadales. ...
Acinetobacter baumannii. *Actinobacteria. *Agrobacterium. *Agrobacterium tumefaciens. *Alcaligenes eutrophus. *Anabaena. * ...
Acinetobacter baumannii. Xanthomonadaceae. *Stenotrophomonas maltophilia. Cardiobacteriaceae. *Cardiobacterium hominis *HACEK. ...
Acinetobacter baumannii. Xanthomonadaceae. *Stenotrophomonas maltophilia. Cardiobacteriaceae. *Cardiobacterium hominis *HACEK. ...
Acinetobacter baumannii. Xanthomonadaceae. *Stenotrophomonas maltophilia. Cardiobacteriaceae. *Cardiobacterium hominis *HACEK. ...
Acinetobacter baumannii. Xanthomonadaceae. *Stenotrophomonas maltophilia. Cardiobacteriaceae. *Cardiobacterium hominis *HACEK. ...
Kwa AL,et al.Nebulized colistin in the treatment of pneumonia due to multidrug-resistant Acinetobacter baumannii and ...
Acinetobacter baumannii, addition of antibiotic specific to gram-negative organism is recommended. For Methicillin-resistant ...
Acinetobacter baumannii. Xanthomonadaceae. *Stenotrophomonas maltophilia. Cardiobacteriaceae. *Cardiobacterium hominis *HACEK. ...
Acinetobacter baumannii, and Pseudomonas aeruginosa". Frontiers in Immunology. 6: 595. doi:10.3389/fimmu.2015.00595. PMID ...
Acinetobacter baumannii, Pseudomonas aeruginosa. Grupa gram pozitivnih i gram negativnih bakterija od posebne važnosti je ... ESKAPE grupa (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas ...
... a version of the enzyme which can use either reduced FAD or reduced FMN has been described in Acinetobacter baumannii. Although ...
Acinetobacter baumannii. *ACT (tes). *Adam. *Adam Air Penerbangan 574. *Adam dan Hawa ...
Acinetobacterസംക്രമണം Acinetobacter baumannii ആക്റ്റിനോ മൈക്കോസിസ് Actinomyces israelii, Actinomyces gerencseriae and ...
Pseudomonas aeruginosa (Pseudomonas infection) · Moraxella catarrhalis · Acinetobacter baumannii. Xanthomonadales. ...
Acinetobacter baumannii. Xanthomonadaceae. *Stenotrophomonas maltophilia. Cardiobacteriaceae. *Cardiobacterium hominis *HACEK. ...
Acinetobacter baumannii. Xanthomonadaceae. *Stenotrophomonas maltophilia. Cardiobacteriaceae. *Cardiobacterium hominis *HACEK. ...
Acinetobacter baumannii. Xanthomonadaceae. *Stenotrophomonas maltophilia. Cardiobacteriaceae. *Cardiobacterium hominis *HACEK. ...
... associated with hospital-acquired infections include Acinetobacter baumannii, which cause bacteremia, ... Acinetobacter baylyi, and gram-negative plant pathogens such as Ralstonia solanacearum and Xylella fastidiosa.[14] ...
Acinetobacter baumannii. Xanthomonadaceae. *Stenotrophomonas maltophilia. Cardiobacteriaceae. *Cardiobacterium hominis *HACEK. ...
MDR strains of Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii have become of most concern because they ... Acinetobacter baumannii, Klebsiella pneumoniae and Stenotrophomonas maltophilia. Also, there has been interest in the drug ...
... baumannii is commonly involved in nosocomial infections, but few therapeutic options are available. Find out what there is to ... Acinetobacter baumannii is one of the most frequently isolated bacteria in the ICU but significant regional differences exist. ... Acinetobacter baumannii is a gram-negative aerobic bacillus that primarily causes hospital-acquired infections affecting ... Purpose of review: We reviewed recent data about epidemiology of Acinetobacter baumannii, resistance mechanisms, and ...
ECDC highlights need for increased efforts to prevent carbapenem-resistant A. baumannii outbreaks Acinetobacter baumannii is ...
Acinetobacter baumanni. ›Acinetobacter baumannii Bouvet and Grimont 1986. ›Acinetobacter genomosp. 2. More ». ›Acinetobacter ... Acinetobacter. › Acinetobacter calcoaceticus/baumannii complex. Strains i. › 252. › ATCC 17945 / CIP 70.22. › ATCC 17961 / CIP ... Acinetobacter sp. NCTC 7422. ›Bacterium anitratum. ›CCUG 19096. ›CIP 70.34. ›DSM 30007. ›JCM 6841. ›LMG 1041. ›LMG:1041. ›NCCB ... Acinetobacter baumannii. Taxonomy navigation. › Acinetobacter calcoaceticus/baumannii complex. Choose one. > Acinetobacter ...
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... Designation: S2 TypeStrain=False Application: Assay of cefoperazone Assay of sulbactam ... Acinetobacter baumannii (ATCC® 43498™) Strain Designations: S2 / Type Strain: no / Biosafety Level: 2 ...
Each antibiotic is presented in three columns. The first column lists the name of the antibiotic. The middle column represents susceptibility in percent to that antibiotic. The 3rd column represents the number of isolates tested for that specific antibiotic ...
Inactivation of phospholipase D diminishes Acinetobacter baumannii pathogenesis.. Jacobs AC1, Hood I, Boyd KL, Olson PD, ... Acinetobacter baumannii is an emerging bacterial pathogen of considerable health care concern. Nonetheless, relatively little ... B) Chromosomal organization of A. baumannii strain ATCC 17978 locus A1S_2989 with the strain ACJ2 transposon EZ-Tn5 ,R6Kγori/ ... Plotted are the numbers of CFU ml of A. baumannii−1 internalized following 4 h of incubation. (B) Internalization properties of ...
This volume serves researchers studying the Gram-negative bacterium Acinetobacter baumannii with chapters detailing ... Acinetobacter baumannii. Methods and Protocols Editors: Biswas, Indranil, Rather, Philip N. (Eds.) ... Authoritative and cutting-edge, Acinetobacter baumannii: Methods and Protocols aims to ensure successful results in the further ... Assessing Acinetobacter baumannii Virulence and Persistence in a Murine Model of Lung Infection ...
Genomic DNA from Acinetobacter baumannii strain AYE (ATCC ® BAA-1710™) TypeStrain=False Application: ... Acinetobacter baumannii (ATCC® BAA-1710D-5™) Strain Designations: Genomic DNA from Acinetobacter baumannii strain AYE (ATCC® ... Acinetobacter baumannii plasmid p2ABAYE, complete sequence Nucleotide (GenBank) : NC_010410 Acinetobacter baumannii AYE, ... Acinetobacter baumannii ATCC® BAA-1710D-5™ freeze-dried Total DNA: At least 5 µg in 1X TE buffer. OD260/OD280: 1.6 to 2.0 ...
Acinetobacter baumannii is an opportunistic pathogen operating in hospitals creating serious infections such as pneumonia. It ... baumannii is resistant to many antibiotics. Furthermore, once a specific course of treatment is prescribed for A. Baumannii, ... That is to say, the option of the researchers has been to try to improve control with respect to A. baumannii given that there ... This means that all the resistances acquired by a strain of A. baumannii can be transmitted to another and the species can thus ...
Objectives: The objectives of this study were to study the presence of mutators in a set of Acinetobacter baumannii isolates ... Prevalence of hypermutators among clinical Acinetobacter baumannii isolates. Komp Lindgren, Patricia Uppsala University, ... Methods: The variation in mutation rate was evaluated for 237 clinical A. baumannii isolates by determining the frequency of ...
Genomic DNA from Acinetobacter baumannii strain 2208 TypeStrain=True Application: Food testing ... Acinetobacter baumannii Bouvet and Grimont (ATCC® 19606D-5™) Strain Designations: Genomic DNA from Acinetobacter baumannii ... Acinetobacter baumannii Bouvet and Grimont ATCC® 19606D-5™ dried At least 5 µg in 1X TE buffer ... Nucleotide (GenBank) : AF100557 Acinetobacter baumannii DNA gyrase A (gyrA) gene, partial cds. ...
A. baumannii is an opportunistic infection that commonly "enters" through a vent, open wound (usually dressed/covered in ... acinetobacter baumannii epidemic in Maryland hospital. page: 1 #liveFeed1, #liveFeed2, #liveFeed3 { margin: 40px 2px 50px 4px; ...
This topic contains 2 study abstracts on Acinetobacter baumannii infection indicating that the following substances may be ... 1 Abstracts with Acinetobacter baumannii infection & Raspberry root Research. [x] Remove Focus on Raspberry root. Filter by ... Diseases : Anthrax, Bacillus anthracis, Carbapenem-resistant Acinetobacter baumannii (CRAB), MRSA. Pharmacological Actions : ... 1 Problem Substances Researched for Acinetobacter baumannii infection Name. AC. CK. Focus. ...
Carbapenem-resistant Acinetobacter baumannii (CRAB)Anti-Tumor,Tumors,Iodine,Lectin-Induced Cancer,Vegetables: All,Thyroid ... Carbapenem-resistant Acinetobacter baumannii (CRAB) is a Sub of the following Topics. *Acinetobacter baumannii infection ... Carbapenem-resistant Acinetobacter baumannii (CRAB) Related Articles. Could Turmeric Save Us From The CDCs Nightmare Bacteria ... 1 Abstracts with Carbapenem-resistant Acinetobacter baumannii (CRAB) Research. Filter by Study Type. In Vitro Study. ...
Background We describe the prevalence of invasive carbapenem-resistant Acinetobacter spp. isolated from 2005 to 2016 in ... Genotypic and phenotypic characterization of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex with the proposal ... formerly Acinetobacter genomic species 3) and Acinetobacter nosocomialis sp. nov. (formerly Acinetobacter genomic species 13TU ... which belong to the so-called Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex. Generally, these four species ...
3ZPG: Structure of Diaminohydroxyphosphoribosylaminopyrimidine Deaminase/5-Amino-6-(5-Phosphoribosylamino)Uracil Reductase from Acinetobacter Baumannii.
T. D. Gootz and A. Marra, "Acinetobacter baumannii: an emerging multidrug-resistant threat," Expert Review of Anti-Infective ... B. A. Eijkelkamp, K. A. Hassan, I. T. Paulsen, and M. H. Brown, "Investigation of the human pathogen Acinetobacter baumannii ... Cloning, Expression, and Purification of Nucleoside Diphosphate Kinase from Acinetobacter baumannii. Juhi Sikarwar, Sanket ... A. Howard, M. ODonoghue, A. Feeney, and R. D. Sleator, "Acinetobacter baumannii: an emerging opportunistic pathogen," ...
... industrial applications and more information for Acinetobacter baumannii. ... Acinetobacter baumannii is an opportunistic pathogen and is a problem in the hospital setting in US and Europe. Many strains ... Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Moraxellaceae; Acinetobacter. Industrial uses or economic ...
KEGG Orthology (KO) - Acinetobacter baumannii AYE [ Brite menu , Organism menu , Download htext , Download json ] ...
Acinetobacter baumannii is an opportunistic bacterial pathogen which causes infections in immunosuppressed patients. ... Survival of Acinetobacter baumannii in different water types. Svjetlana Dekić ; Faculty of Science, University of Zagreb, ... "Preživljavanje bakterije Acinetobacter baumannii u različitim tipovima vode." Hrvatske vode, vol. 26, br. 103, 2018, str. 35-38 ... S. Dekić i J. Hrenović, "Preživljavanje bakterije Acinetobacter baumannii u različitim tipovima vode", Hrvatske vode, vol.26, ...
... E. ... "Mutant Prevention Concentrations of Imipenem and Meropenem against Pseudomonas aeruginosa and Acinetobacter baumannii," The ...
... Michal Jarmoluk / Pixabay ... Acinetobacter baumannii bacteria is known to cause pneumonia or serious blood or wound infections. ... Following the childs death, the state Department of Health also discovered four cases of Acinetobacter baumannii infection in ... Wade said that acinetobacter is a hospital-acquired infection with no community transmission, which means that it does not pose ...
In laboratory experiments, we compared the abilities of five Acinetobacter baumannii strains, three Acinetobacter sp. strains ... Survival of Acinetobacter baumannii on dry surfaces. Message Subject (Your Name) has forwarded a page to you from Journal of ... Survival of Acinetobacter baumannii on dry surfaces.. C Wendt, B Dietze, E Dietz, H Rüden ... Acinetobacter spp. have frequently been reported to be the causative agents of hospital outbreaks. The circumstances of some ...
Inhaled colistin on Acinetobacter baumannii treatment. Gustavo Coimbra dos Reis, Ana Martins, Luís Bento ... Inhaled colistin on Acinetobacter baumannii treatment Message Subject (Your Name) has sent you a message from European ... A. baumannii was extensively resistant in all cases, being sensible only to polymyxins in 20 (87%). Mean inhaled colistin diary ... Conclusions: On critical patients, inhaled colistin was effective treating PAV/TAV due to extensively resistant A. baumannii. ...
KEGG Orthology (KO) - Acinetobacter baumannii MDR-TJ [ Brite menu , Organism menu , Download htext , Download json ] ...
... baumannii strains from Australia. Twitching motility was found to be a common tr … ... Acinetobacter baumannii continues to be a major health problem especially in hospital settings. Herein, features that may play ... Acinetobacter baumannii continues to be a major health problem especially in hospital settings. Herein, features that may play ... Adherence and motility characteristics of clinical Acinetobacter baumannii isolates FEMS Microbiol Lett. 2011 Oct;323(1):44-51. ...
Acinetobacter baumannii is the cause of difficult-to-treat infections in healthcare settings in Europe due to its increasing ... Outbreaks of carbapenem-resistant Acinetobacter baumannii in healthcare facilities have been reported in Europe and worldwide. ... Efforts Needed to Stop the Spread of Acinetobacter Baumannii Infections in Europe. ... there has been an overall increase of carbapenem-resistant Acinetobacter baumannii in Europe. ...
Identification of Acinetobacter baumannii by detection of the blaOXA-51-like carbapenemase gene intrinsic to this species. J. ... Acinetobacter baumannii is an opportunistic pathogen associated with severe hospital infections (1, 16), which often need the ... Novel acquired metallo-ß-lactamase gene, blaSIM-1, in a class 1 integron from Acinetobacter baumannii clinical isolates from ... Heteroresistance to Meropenem in Carbapenem-Susceptible Acinetobacter baumannii Alexandros Ikonomidis, Evangelia Neou, ...
  • To identify virulence factors that may contribute to these disease processes, genetically diverse A. baumannii clinical isolates were evaluated for the ability to proliferate in human serum. (nih.gov)
  • All clinical isolates were confirmed to be A. baumannii by Microscan Identification Panel assessment (data not shown) and were subjected to PFGE to assess their clonality. (nih.gov)
  • The objectives of this study were to study the presence of mutators in a set of Acinetobacter baumannii isolates and to explore whether there is a correlation between mutation rates and antibiotic resistance. (diva-portal.org)
  • The variation in mutation rate was evaluated for 237 clinical A. baumannii isolates by determining the frequency of their mutation to rifampicin resistance. (diva-portal.org)
  • Despite a relatively stable number of carbapenem-resistant Acinetobacter isolates in Switzerland, our results suggest the existence of a diverse pool of A. baumannii species in hospital settings, and confirm the implication of carbapenem-resistant Acinetobacter calcoaceticus - Acinetobacter baumannii (ACB) complex in the vast majority of clinical infections and nosocomial outbreaks with notable regional fluctuations. (springer.com)
  • Three A. baumannii isolates with multiple antibiotic resistance multiply were inoculated in spring water, sea water and wastewater treatment plant effluent and incubated on 20°C in the period of 50 days. (srce.hr)
  • Twitching motility was found to be a common trait in A. baumannii international clone I strains and in abundant biofilm formers, whereas swarming motility was only observed in isolates not classified within the international clone lineages. (nih.gov)
  • We report for the first time the motility characteristics of a large number of A. baumannii isolates and present a direct comparison of A. baumannii binding to nasopharyngeal and lung epithelial cells. (nih.gov)
  • The characteristics of carbapenem heteroresistance were studied in 14 apparently carbapenem-susceptible Acinetobacter baumannii isolates. (asm.org)
  • However, reduced susceptibility or resistance to carbapenems is increasingly being observed among A. baumannii clinical isolates ( 2 , 5 , 25 ). (asm.org)
  • Likewise, a recent study ( 12 ) has described colistin-heteroresistant subpopulations among apparently colistin-susceptible A. baumannii clinical isolates, implying an intrinsic potential of the species to overcome drug pressure. (asm.org)
  • The present study aimed to evaluate in vitro the incidence of carbapenem heteroresistance, to characterize the traits of the heteroresistant subpopulations, and to assess the efficacies of carbapenems against heteroresistant A. baumannii isolates. (asm.org)
  • All single A. baumannii clinical isolates that were recovered in the University Hospital of Larissa (Larissa, Greece), a tertiary-care hospital with 700 beds, over a 6-month period from September 2007 to March 2008 were recorded. (asm.org)
  • Pulsed-field gel electrophoresis of Apa I restricted analysis of Acinetobacter baumannii isolates. (cdc.gov)
  • A. baumannii MOS-1 was not clonally related to well-characterized PER-1 A. baumannii strains from Turkey, France, and Belgium ( 4 , 9 ) ( Figure ), further supporting genetic heterogeneity of PER-1 A. baumannii isolates, even though the immediate genetic environment of bla PER-1 gene was similar ( 8 ). (cdc.gov)
  • In May 2020, hospital A notified NJDOH of an increase in CRAB ( A. baumannii with meropenem minimum inhibitory concentration testing of ≥8 μ g/mL) isolates from weekly ICU point prevalence surveys (colonization screening) and from clinical infections. (cdc.gov)
  • Two MDR A. baumannii isolates (ATCC 17978 and AB 251847) were separately cultured in tryptic soy broth (∼6 log CFU/ml) containing the minimum inhibitory concentration (MIC) of TC or EG with or without the MIC of each antibiotic at 37°C for 18 h. (frontiersin.org)
  • The aim of the present study is to assess the prevalence of extended spectrum β-lactamases (ESBLs) and AmpC β-lactamases among isolates of A. baumannii collected from different clinical sources in Mansoura University Hospitals, Egypt. (springer.com)
  • The study demonstrates high prevalence of resistance to β-lactam antibiotics through ESBLs and AmpC β-lactamases production among A. baumannii clinical isolates. (springer.com)
  • The genetic basis of antimicrobial resistance in Bolivia has not been deeply studied until now, and the mobilome of these A. baumannii isolates, combined with their multi-drug resistant phenotype, mirror the transfer and prevalence of MGEs contributing to the spread of antibiotic resistance worldwide and require special attention. (frontiersin.org)
  • While A. baumannii has been isolated from the wider environment such as water, soil, and animals, most studied isolates come from clinical samples, where A. baumannii has become a serious health problem, particularly in the intensive care unit, where it can cause serious and prolonged outbreaks ( Gonzalez-Villoria and Valverde-Garduno, 2016 ). (frontiersin.org)
  • Okasha, H. and Meheissen, M. (2017) In Vitro Activity of Colistin and Vancomycin or Azithromycin Combinations on Extensively Drug Resistant Acinetobacter baumannii Clinical Isolates. (scirp.org)
  • Of 96 A. baumannii clinical isolates, 66 strains carried class 1 integrons (no class 2 or 3 integrons were found). (ebscohost.com)
  • This study was conducted to determine the prevalence and the local antibiogram of multidrug-resistant Acinetobacter baumannii isolates in Al-Hussein Hospital at King Hussein Medical Centre in Amman, Jordan. (ebscohost.com)
  • The rapid detection of antibiotic resistance in clinical isolates of Acinetobacter baumannii would shorten the period during which patients receive empirical therapy and facilitate the early initiation of directed antibiotic therapy. (ovid.com)
  • The objective of this study was to evaluate the ability of a real-time PCR assay to detect antibiotic resistance to four clinically relevant antibiotics from different antibiotic classes in clinical isolates of A. baumannii. (ovid.com)
  • The growth of 48 clinical isolates of A. baumannii with a broad range of MICs of imipenem, ciprofloxacin, colistin and amikacin was evaluated using a real-time PCR assay targeting a highly conserved region of the ompA gene. (ovid.com)
  • Despite a relatively low virulence of Acinetobacter baumannii isolates, emerging multidrug-resistant (MDR) strains to pose a formidable threat to patients, particularly in ill patients in intensive care unit (ICU). (magiran.com)
  • Fifty-five non-repetitive A. baumannii isolates were examined for antimicrobial susceptibility, oxacillinase genes, class 1 integrons and genetic relationships by PFGE. (magiran.com)
  • The present study examined the occurrence of the genetic elements bla OXA and IS Aba 1 and their relationship with susceptibility to carbapenems in clinical isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. (scielo.br)
  • The presence of the IS Aba 1 sequence upstream of the bla OXA-23-like gene was strongly associated with carbapenem resistance in isolates of the A. calcoaceticus-A. baumannii complex in the hospital center studied. (scielo.br)
  • Isolates were identified by using the morphology of the colonies in selective media, MicroScan and the ability to grow at 44degrees C. Risk factors of the patients with or without nosocomial acquisition of A. baumannii were compared. (koreamed.org)
  • DNA hybridization experiments showed that the presence of strain 19606 genes encoding regulatory and attachment or biofilm functions is widespread among other A. baumannii clinical isolates. (asm.org)
  • Literature searches showed that there are numerous reports describing either the different types of infections caused by A. baumannii , the antibiotic resistance profiles of the different clinical strains, the characterization of some of the genetic elements responsible for their antibiotic resistance, or the development and application of typing methods and fingerprinting systems used to identify and trace the sources of clinical isolates of this pathogen. (asm.org)
  • Most of the A. baumannii isolates were obtained from intensive care unit (ICU) ward of hospitals. (eurekaselect.com)
  • In contrast to their synergistic effect against carbapenem-resistant A. baumannii isolates, colistin and tigecycline were highly antagonistic to carbapenem-resistant A. baumannii strains isolated from patients with VAP when the drugs were administered together. (medsci.org)
  • These diseases involve a group of isolates represented by members of the " A. calcoaceticus-A. baumannii (Acb) complex," which are relevant to human health since they are associated with intra- and extranosocomial infections worldwide ( 9 ). (asm.org)
  • Fifty clinical isolates of multidrug-resistant (MDR) A. baumannii were analyzed for clonal relatedness, serum resistance, biofilm formation, and in vivo assays. (springer.com)
  • Lee HW, Koh YM, Kim J et al (2008) Capacity of multidrug-resistant clinical isolates of Acinetobacter baumannii to form biofilm and adhere to epithelial cell surfaces. (springer.com)
  • Mirshekar M, Shahcheraghi F, Azizi O et al (2018) Diversity of class 1 integrons, and disruption of carO and dacD by insertion sequences among Acinetobacter baumannii isolates in Tehran, Iran. (springer.com)
  • In 2004 and 2005, 5 metallo-beta-lactamase (MBL)-positive Acinetobacter baumannii isolates were found in 2 Greek hospitals. (cdc.gov)
  • Whole genome sequencing of clinical isolates of Acinetobacter baumannii collected in Japan was performed. (nii.ac.jp)
  • Comparative genome-wide association studies using A. baumannii isolates collected and isolates in public databases was performed to identify genetic traits of the pandemic lineages. (nii.ac.jp)
  • The specimens were characterized as A .baumannii by conventional phenotypic and biochemical tests.The isolates were confirmed by PCR for OXA_51 like gene. (sid.ir)
  • Findings: From 350 specimens, 43 isolates was Acinetobacter baumannii .The antimicrobial patterns of isolates showed that 53.5% of isolates were resistant to amikacin, 83.7% to tetracyclin, 86% to ceftazidime, 90.7% to trimetoprim sulfametoxazol, 93% to imipenem, cefepime, meropenem, ampicillin -Sulbactam. (sid.ir)
  • The following numbers of clinical isolates were tested: Enterococcus faecalis (n = 135), Enterococcus faecium (n = 227), P. aeruginosa (n = 295) and A. baumannii (n = 158). (deepdyve.com)
  • This study determined the prevalence of MDR A. baumannii isolates from intensive care units in a large tertiary-care hospital in Ismailia, Egypt, and the occurrence of different beta-lactamases in these isolates. (who.int)
  • A. baumannii has also been identified as an ESKAPE pathogen (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species), a group of pathogens with a high rate of antibiotic resistance that are responsible for the majority of nosocomial infections. (wikipedia.org)
  • Acinetobacter baumannii is an emerging bacterial pathogen of considerable health care concern. (nih.gov)
  • Acinetobacter baumannii is an opportunistic pathogen operating in hospitals creating serious infections such as pneumonia. (innovations-report.com)
  • Furthermore, once a specific course of treatment is prescribed for A. Baumannii, the pathogen has a great capacity for acquiring resistance to these antibiotics. (innovations-report.com)
  • The pathogen also has other options for their acquisition but it is the integrons that provide the most efficacious way to acquire and transmit the resistances, given that, apart from acquiring resistances, integrons have great mobility and can transfer from one location of the A. baumannii chromosome to another. (innovations-report.com)
  • Acinetobacter baumannii is an opportunistic pathogen and is a problem in the hospital setting in US and Europe. (thelabrat.com)
  • Acinetobacter baumannii is an opportunistic bacterial pathogen which causes infections in immunosuppressed patients. (srce.hr)
  • Considering insufficient data on the survival success rate of this pathogen in the natural environment, the purpose of this paper was to determine the A. baumannii survival rate in different water types. (srce.hr)
  • Acinetobacter baumannii is an opportunistic pathogen associated with severe hospital infections ( 1 , 16 ), which often need the use of carbapenems as the treatment of last resort. (asm.org)
  • Carbapenem-resistant Acinetobacter baumannii (CRAB), an opportunistic pathogen primarily associated with hospital-acquired infections, is an urgent public health threat ( 1 ). (cdc.gov)
  • Multi-drug resistant (MDR) Acinetobacter baumannii is a major nosocomial pathogen causing a wide range of clinical conditions with significant mortality rates. (frontiersin.org)
  • In this work, we show that the opportunistic pathogen Acinetobacter baumannii senses and responds to blue light. (asm.org)
  • Acinetobacter baumannii is an opportunistic pathogen that has been held responsible for a lot of infections worldwide. (springer.com)
  • Acinetobacter baumannii has emerged as an opportunistic pathogen among acutely ill patients, especially those with thermal injury. (cambridge.org)
  • Here we provide evidence that DHA, an omega-3 fatty acid, has superior antimicrobial effects upon the highly drug-resistant pathogen Acinetobacter baumannii , thereby providing insights into one of the potential health benefits of omega-3 fatty acids. (asm.org)
  • Attenuation of quorum sensing in the pathogen Acinetobacter baumannii using non-native N-Acyl homoserine lactones. (sigmaaldrich.com)
  • Acinetobacter baumannii has emerged as a pan-drug-resistant pathogen and displays a remarkable ability to persist in hospital settings despite desiccation and antimicrobial treatment. (sigmaaldrich.com)
  • We sought to identify non-native ligands capable of blocking or promoting AbaR activity in A. baumannii for use as chemical probes to modulate QS phenotypes in this pathogen. (sigmaaldrich.com)
  • In this study, we screened an open-source Pathogen Box library for anti- A. baumannii compounds. (mmv.org)
  • Purpose: As an opportunistic pathogen, Acinetobacter baumannii causes various nosocomial infections. (ebscohost.com)
  • We have characterized the kinetic properties of FolD from the Gram-negative pathogen Acinetobacter baumanni and determined high-resolution crystal structures of complexes with a cofactor and two potent inhibitors. (biomedsearch.com)
  • The opportunistic pathogen Acinetobacter baumannii is able to persist in the environment and is often multidrug resistant (MDR), causing difficulties in the treatment of infections. (asm.org)
  • IMPORTANCE Acinetobacter baumannii is a nosocomial pathogen and is an increasing problem in hospitals worldwide. (asm.org)
  • Acinetobacter baumannii is an opportunistic pathogen that commonly causes nosocomial infections, such as ventilator-associated pneumonia and skin, soft tissue, wound, and bloodstream infections ( 1 ). (asm.org)
  • The bacterial genus Acinetobacter is recognized as a key HAI-causing pathogen that is associated with high mortality rates in hospital settings 2 ) ( 4 . (scielo.br)
  • BACKGROUND: Acinetobacter baumannii is an important nosocomial pathogen being reported with increasing frequency in outbreaks during the past decade. (koreamed.org)
  • The ability of Acinetobacter sp to form biofilms, resist desiccation and persist on hospital surfaces has played a critical role in the emergence of this bacterium as a human pathogen. (hud.ac.uk)
  • The ability of clinical strains of A. baumannii to form strong adherent biofilms has also been recognised as a key virulence factor for this pathogen. (hud.ac.uk)
  • Acinetobacter baumannii is a metabolically versatile pathogen that causes severe infections in compromised patients. (asm.org)
  • Acinetobacter baumannii is being increasingly recognized as an important pathogen that causes severe infections in hospitalized patients ( 6 , 7 ). (asm.org)
  • Acinetobacter baumannii (A. baumannii) is an important opportunistic pathogen that causes major public health concerns, especially in hospitalized patients due to acquisition of resistant genes. (eurekaselect.com)
  • Paterson, D.L. Acinetobacter baumannii: emergence of a successful pathogen. (eurekaselect.com)
  • All these observations provide strong evidence showing the capacity of A. baumannii to sense light and interact with biotic and abiotic surfaces using undetermined alternative sensing and regulatory systems as well as alternative adherence and motility cellular functions that allow this pathogen to persist in different ecological niches. (asm.org)
  • As a facultative pathogen that lives in different ecological niches, A. baumannii uses a wide range of extracellular signals to adapt to either an environmental or a host lifestyle, with each style playing a critical role in the less understood life cycle and ecology of this bacterium. (asm.org)
  • Background: Acinetobacter baumannii is one of the most important pathogen in hospital acquired infections especially in intensive care units (ICUs). (sid.ir)
  • A.baumannii as a nosocomial opportunistic pathogen is resistant to wide range of antibiotics. (sid.ir)
  • In order to achieve this improved control, it is essential to detect the infection in time and know if A. baumannii has produced it. (innovations-report.com)
  • A. baumannii is an opportunistic infection that commonly "enters" through a vent, open wound (usually dressed/covered in hospital or covered via a wound vac) or PICC site (Peripherally Inserted Central Catheter, Midline or Mediport aka IV line). (abovetopsecret.com)
  • Following the child's death, the state Department of Health also discovered four cases of Acinetobacter baumannii infection in the NICU of the hospital since October. (inquisitr.com)
  • Wade said that acinetobacter is a hospital-acquired infection with no community transmission, which means that it does not pose risk of spreading in the Newark community. (inquisitr.com)
  • No randomized trials have been performed to specifically evaluate combination antimicrobial drug therapy for treatment of infection with A . baumannii . (cdc.gov)
  • Numerous outbreaks of A . baumannii infection have been reported worldwide ( 5 ). (cdc.gov)
  • Therefore, we alert healthcare workers to the need for stringent care in adhering to infection control precautions when caring for patients infected with XDR A . baumannii . (cdc.gov)
  • After 1 week, he was transferred to the ICU for multiple organ failure related to a nosocomial infection caused by an A. baumannii strain susceptible to several antimicrobial drugs, including imipenem (with positive lung aspiration and blood cultures). (cdc.gov)
  • Carbapenem-resistant Acinetobacter baumannii (CRAB) causes health care-associated infections that are challenging to contain and often linked to infection prevention and control (IPC) breaches. (cdc.gov)
  • Body lice that fed on rabbits infused with each Acinetobacter species demonstrated a generalized infection. (ajtmh.org)
  • Ecological aspects and prophylaxis of Acinetobacter baumannii nosocomial infection. (ajtmh.org)
  • In recent years, the increasing cumulative infection outbreaks involving A. baumannii have appeared worldwide. (ebscohost.com)
  • Anti-multidrug-resistant Acinetobacter baumannii activity of DS-8587: In vitro activity and in vivo efficacy in a murine calf muscle infection model. (sigmaaldrich.com)
  • These data indicate DS-8587 would be an effective agent against MDR A. baumannii infection. (sigmaaldrich.com)
  • Kawai, K. Carbapenem resistance and mortality in patients with Acinetobacter baumannii infection: systematic review and meta-analysis. (eurekaselect.com)
  • Xiao D, Wang L, Zhang D, Xiang D, Liu Q, Xing X. Prognosis of patients with Acinetobacter baumannii infection in the care unit: a retrospective analysis. (medigraphic.com)
  • In this study, using the mouse model of intraperitoneal (i.p.) mucin-enhanced A. baumannii infection, we characterized the kinetics of bacterial replication and dissemination and the host innate immune responses, as well as their potential contribution to mucin-enhanced bacterial virulence. (asm.org)
  • A. baumannii is a significant cause of bacterial bloodstream infection in humans. (asm.org)
  • Despite the wide range of studies on green synthesis of silver nanoparticles, there is currently no alternative treatment for MDR A. baumannii infection. (eurekamag.com)
  • This study investigated the potential of silver nanoparticles synthesized with Eucalyptus critriodora leaf extract as an inhibitor of MDR A. baumannii infection. (eurekamag.com)
  • The synthesized silver nanoparticles inhibited MDR A. baumannii infection by approximately 90% without cytotoxicity with a 50% effective concentration of 0.028 μg/ml. (eurekamag.com)
  • Resistant genes could coexist in one strain, therefore, strict implementation of infection control measures is essential to avoid the rapid spread or outbreaks of these multidrug-resistant Acinetobacter baumannii in healthcare-associated facilities. (minervamedica.it)
  • Despite the low prevalence of A. baumannii infection in this hospital, the antibiotic resistance profile suggests that prevention of health-care-associated transmission of MDR Acinetobacter spp. (who.int)
  • The retrospective study included 90 patients with exacerbation of COPD whose etiological factor of infection was A. baumannii , hospitalized in the Department of Pulmonology (MSS) in 2012-2016. (aaem.pl)
  • 05). Conclusion 3 hours of Imipenem/cilastatin infusion can improve the efficacy in treatment of severe pan-resistant patients with Acinetobacter baumannii infection,and it is suitable for applying to clinical treatment. (cnki.com.cn)
  • Hombach, Michael;Jetter, Marion;Blöchliger, Nicolas;Kolesnik-Goldmann, Natalia;Keller, Peter M;Böttger, Erik C 2018-02-01 00:00:00 Abstract Background We investigated the feasibility of rapid disc diffusion antibiotic susceptibility testing (rAST) with reading of inhibition zones after 6 and/or 8 h of incubation for Enterococcus faecalis, Enterococcus faecium, Pseudomonas aeruginosa and Acinetobacter baumannii. (deepdyve.com)
  • ABSTRACT Multi-drug resistant (MDR) strains of Acinetobacter baumannii are responsible for an increasing number of opportunistic infections in hospitals. (who.int)
  • In the Gram-negative, strictly aerobic Acinetobacter genus, the species that mostly present a risk as opportunistic human pathogens are A. baumannii , A. nosocomialis, A. pittii , A. calcoaceticus , A. seifertii and A. dijkshoorniae, which belong to the so-called Acinetobacter calcoaceticus - Acinetobacter baumannii (ACB) complex. (springer.com)
  • Collectively, these results suggest that PLD is an A. baumannii virulence factor. (nih.gov)
  • Zarrilli, R. Virulence-related traits of epidemic Acinetobacter baumannii strains belonging to the international clonal lineages I-III and to the emerging genotypes ST25 and ST78. (eurekaselect.com)
  • Zarrilli, R. Acinetobacter baumannii virulence determinants involved in biofilm growth and adherence to host epithelial cells. (eurekaselect.com)
  • We found that mucin, either admixed with or separately injected with the challenge bacterial inoculum, was able to enhance the tissue and blood burdens of A. baumannii strains of different virulence. (asm.org)
  • Successful clones of Acinetobacter baumannii cause a variety of nosocomial infections through serum resistance, biofilm formation, and antimicrobial resistance as virulence capabilities. (springer.com)
  • Harding CM, Hennon SW, Feldman MF (2018) Uncovering the mechanisms of Acinetobacter baumannii virulence. (springer.com)
  • Integrons are chains of genes wherein many of the resistances acquired by the A. baumannii bacteria are found. (innovations-report.com)
  • The bacteria in the effluent multiplied, and the number of A. baumannii was higher than the initial number even after 50 days of monitoring. (srce.hr)
  • The multiplication of bacteria and improved survival of A. baumannii in the effluent in comparison to spring or sea water is attributed to higher nutrient availability. (srce.hr)
  • Acinetobacter baumannii bacteria is known to cause pneumonia or serious blood or wound infections. (inquisitr.com)
  • Acinetobacter baumannii bacteria, coloured scanning electron micrograph (SEM). (sciencephoto.com)
  • Multi-drug resistant Acinetobacter baumannii bacteria inside biofilm, computer illustration. (sciencephoto.com)
  • During 2018, CDC was notified of a rare case of organ-donor derived transmission of a multidrug-resistant bacteria (OXA-23-producing carbapenem-resistant Acinetobacter baumannii) during lung transplantation. (cdc.gov)
  • Bacteria of the Acinetobacter genus. (ajtmh.org)
  • Background: Extensively drug resistant Acinetobacter baumannii (XDR-AB) presents an increasing challenge to health care in Egypt as they are among the most common bacteria isolated in hospital setting. (scirp.org)
  • A. baumannii 19606 derivatives with defective attachment or biofilm phenotypes had insertions within genes that appear to be part of a chaperone-usher transport system described for other bacteria. (asm.org)
  • Background: Carbapenem-resistant Acinetobacter baumannii (A. baumannii) was on the top of the list of the most threatening bacteria published by the WHO in 2017. (eurekaselect.com)
  • Conclusion: In conclusion, our research is the first to demonstrate that CADY can deliver ASOs into bacteria and provide a novel strategy for the treatment of MDR-A. baumannii. (eurekaselect.com)
  • Acinetobacter bacteria are Gram-negative, aerobic, non-spore-forming bacteria that do not require complex media for growth ( 1 ). (asm.org)
  • In fact, some strains of bacteria are resistant to all available antibiotics, such as Acinetobacter baumannii , which is the focus of this report. (asm.org)
  • A. baumannii is part of the ACB complex (A. baumannii, A. calcoaceticus, and Acinetobacter genomic species 13TU). (wikipedia.org)
  • Acinetobacter calcoaceticus subsp. (atcc.org)
  • Dimarco AA, Averhoff B, Ornston LN (1993) Identification of the transcriptional activator pobR and characterization of its role in the expression of pobA , the structural gene for p-hydroxybenzoate hydroxylase in Acinetobacter calcoaceticus . (springer.com)
  • Consequently, numerous HAI outbreaks caused by multidrug-resistant (MDR) representatives of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex have been reported in several countries, including Brazil 2 ) ( 5 ) ( 6 . (scielo.br)
  • Material and Methods: Observational, longitudinal, retrospective study, through file consult, of patients admitted at UUM from 01/2005 to 12/2011, with A. baumannii VAP/TAV, treated with inhaled colistin. (ersjournals.com)
  • Conclusions: On critical patients, inhaled colistin was effective treating PAV/TAV due to extensively resistant A. baumannii. (ersjournals.com)
  • While carbapenems traditionally were the antibiotics of choice for treatment of A. baumannii infections, resistance to these drugs has led to increased use of colistin as last-line treatment. (medindia.net)
  • Although still rare, resistance to colistin in A. baumannii is also increasingly being reported in Europe. (medindia.net)
  • These observations suggest that the use of carbapenems or colistin to treat severe multidrug-resistant A. baumannii infections may lead to the development of resistance. (asm.org)
  • On the day of admission, bacterial cultures taken from sputum showed a multidrug-resistant A. baumannii MOS-1 strain, susceptible only to colistin and rifampin. (cdc.gov)
  • A. baumannii MOS-1 was susceptible to colistin and rifampin only, and no synergy image could be observed between clavulanic acid and cefepime or ceftazidime discs on a routine antibiogram performed by the disc diffusion method. (cdc.gov)
  • Cikman A, Gulhan B, Aydin M, Ceylan MR, Parlak M, Karakecili F, Karagoz A. In vitro Activity of Colistin in Combination with Tigecycline against Carbapenem-Resistant Acinetobacter baumannii Strains Isolated from Patients with Ventilator-Associated Pneumonia. (medsci.org)
  • This study investigated the minimum inhibitory concentration (MIC) values and in vitro activity of colistin in combination with tigecycline against carbapenem-resistant Acinetobacter baumannii strains isolated from patients with ventilator-associated pneumonia (VAP) using the E-test method. (medsci.org)
  • While only 1 of 40 A. baumannii strains was determined to be colistin resistant, 6 were tigecycline resistant. (medsci.org)
  • The MIC 50 , MIC 90 , and MIC intervals of the A. baumannii strains were 0.19, 1.5, and 0.064‒4 μg/ml for colistin and 1, 8, and 0.094‒256 μg/ml for tigecycline, respectively. (medsci.org)
  • In this study we explored alternative output parameters to assess the activities of colistin and meropenem against Pseudomonas aeruginosa and Acinetobacter baumannii. (diva-portal.org)
  • Methods: Four strains each of P. aeruginosa and A. baumannii were exposed to colistin and meropenem, alone and in combination, in 8h dynamic time-kill experiments. (diva-portal.org)
  • Conclusions: Combination effects were frequently found with colistin and meropenem against P. aeruginosa and A. baumannii in time-kill experiments but were not detected with the checkerboard method. (diva-portal.org)
  • A. baumannii in our hospital were highly resistant to all antibiotics, including tigecycline, except for minocycline and colistin which are considered the last resort treatment for multidrug-resistant A. baumannii. (who.int)
  • Z96926 Acinetobacter baumannii waaA gene, strain ATCC 19606. (atcc.org)
  • These bacterial responses depend on the expression of the A. baumannii ATCC 17978 A1S_2225 gene, which codes for an 18.6-kDa protein that contains an N-terminal b lue- l ight-sensing- u sing f lavin (BLUF) domain and lacks a detectable output domain(s). (asm.org)
  • A. baumannii cells were procured from MicroBioLogics in sealed packs bearing the American Type Culture Collection (ATCC 19606) number and stored according to the recommended storage protocols until needed for experiments. (omicsonline.org)
  • Transcriptional analyses of Acinetobacter baumannii ATCC 17978 showed that the expression of A1S_2091 was enhanced in cells cultured in darkness at 24°C through a process that depended on the BlsA photoreceptor. (asm.org)
  • A novel, plasmid-mediated, high-level tigecycline resistance tet (X) gene variant, tet (X5), was detected in a clinical Acinetobacter baumannii isolate from China in 2017. (asm.org)
  • An A . baumannii isolate obtained just before she died lacked susceptibility to all commercially available antimicrobial drugs ( Table ). (cdc.gov)
  • Here we describe what we believe is the first ESBL-producing A. baumannii isolate resistant to carbapenems and the first characterization of a PER-1 A. baumannii isolate from Russia, further supporting the emergence and dissemination of PER-1 A. baumannii strains in eastern Europe and outside Turkey ( 3 , 4 ). (cdc.gov)
  • No other multidrug-resistant A. baumannii isolate with a similar resistance profile has been isolated in the hospital before, during, or after this period. (cdc.gov)
  • With blood culture isolate(s) A. baumannii. (clinicaltrials.gov)
  • Complete genome sequence of the siphoviral bacteriophage Βϕ-R3177, which lyses an OXA-66-producing carbapenem-resistant Acinetobacter baumannii isolate. (jcvi.org)
  • No significant association has been found between the pulsotype of each isolate and MDR patterns and the presence of carbapenemase genes, however, highly resistant blaOXA-23 gene carrying endemic clones of A. baumannii disseminated in the ICU of two hospitals. (magiran.com)
  • Further, the effect of TC and EG on the expression of A. baumannii genes encoding resistance to β-lactam antibiotics ( blaP ), efflux pumps ( adeABC ), and multi-drug resistant protein ( mdrp ) was studied using real-time quantitative PCR (RT-qPCR). (frontiersin.org)
  • Using a combination of short- and long-read DNA sequencing, we have investigated the location of antibiotic resistance genes and characterized mobile genetic elements (MGEs) in three clinical multi-drug resistant Acinetobacter baumannii . (frontiersin.org)
  • A diverse range of MGEs have been described in A. baumannii , for example transposons such as Tn2008, Tn2008B , Tn2006 , Tn2009 , or Tn2007 , which represent different transposon configurations carrying the bla OXA-23 gene together with IS Aba1 or IS Aba4 , and additional genes ( Nigro and Hall, 2016 ). (frontiersin.org)
  • After screening on selective agar media, using PCR arrays and whole genome sequencing, we reported for the first time two OXA-23-producing A. baumannii strains belonging to the widespread ST2/International Clone II and harbouring aminoglycoside-modifying enzymes (aac(6')-Ib and aac(3')-I genes). (archives-ouvertes.fr)
  • For example, A. baumannii lacking AdeRS displayed decreased expression of adeABC , pil genes, com genes, and a pgaC -like gene, whereas loss of AdeB resulted in increased expression of pil and com genes and decreased expression of ferric acinetobactin transport system genes. (asm.org)
  • In this investigation we tried to transfer plasmid encoded silver and antibiotic resistance genes from Acinetobacter baumannii BL54 to E. coli k12 J53.2 and Pseudomonas (a soil microflora) by conjugation and transformation in different soil microcosms and study the survival of the isolated bacterium in each soil. (academicjournals.org)
  • Sequence analysis of the recovered plasmids showed that some of the A. baumannii 19606 growth-defective derivatives contain insertions within genes encoding activities required for the generation of energy and cell wall components and for the biosynthesis of amino acids and purines. (asm.org)
  • In addition, CADY-NPs targeting acpP concentrationdependently retarded the growth of MDR-A. baumannii, which was associated with the decreased expression of targeted genes in A. baumannii. (eurekaselect.com)
  • The aim of this study was to systematically review the published data on the prevalence and dispersion of metallo-β-lactamases (MBLs) genes in A. baumannii in different provinces of Iran and provide an overall prevalence rate using meta-analysis. (eurekaselect.com)
  • Based on the results, further attention should be given to report MBL genes in A. baumannii based on molecular detection rather than the phenotypic one. (eurekaselect.com)
  • We investigated aminoglycoside resistance genes in clinical multidrug-resistant Acinetobacter baumannii strains isolated from the Chinese population. (minervamedica.it)
  • However, it is unlikely that new antimicrobial drugs will be available in the near future to treat patients infected with gram-negative pathogens such as Acinetobacter baumannii ( 1 ). (cdc.gov)
  • A crisis is looming should XDR A . baumannii become established pathogens in hospitals. (cdc.gov)
  • Acinetobacter baumannii has been designated as one of the six ESKAPE (Enterococcus, Staphylococcus, Klebsiella, Acinetobacter, Pseudomonas and Enterobacter) bacterial pathogens of greatest healthcare concern. (rochester.edu)
  • Members of the Acinetobacter genus are key pathogens that cause healthcare-associated infections, and they tend to spread and develop new antibiotic resistance mechanisms. (scielo.br)
  • Acinetobacter baumannii is one of the most clinically important nosocomial pathogens. (asm.org)
  • Porcine mucin has been commonly used to enhance the infectivity of bacterial pathogens, including Acinetobacter baumannii , in animal models, but the mechanisms for enhancement by mucin remain relatively unknown. (asm.org)
  • EUCAST and CLSI recommend an incubation time of 16-18 h for most species/drug combinations.13,14 In a proof-of-principle study, we recently demonstrated that automated rAST is feasible.15 We here evaluated whether early reading of disc diffusion is possible for clinical pathogens with a high diversity of intrinsic resistance mechanisms, namely enterococci, P. aeruginosa and A. baumannii. (deepdyve.com)
  • Multi-resistant Acinetobacter baumannii isolated from patients has become one of the most hazardous pathogens in health care settings. (aaem.pl)
  • Moreover, the mortality rate from these infections are usually high given, on the one hand, the weakness of the patient and, on the other, A. baumannii is resistant to many antibiotics. (innovations-report.com)
  • Thus, it is highly probable that A. baumannii becomes resistant to the best antibiotics that exist today and that this resistance is transmitted via integrons. (innovations-report.com)
  • Moreover, A. baumannii strains have been identified that are resistant to the most common antibiotics used today. (innovations-report.com)
  • That is to say, the option of the researchers has been to try to improve control with respect to A. baumannii given that there is currently no substitutes for the antibiotics used to date. (innovations-report.com)
  • A. baumannii strains are equipped with a multitude of antibiotic resistance mechanisms, rendering them resistant to most of the currently available antibiotics. (frontiersin.org)
  • This study investigated the efficacy of two food-grade, plant-derived antimicrobials (PDAs), namely trans -cinnamaldehyde (TC) and eugenol (EG) in decreasing A. baumannii 's resistance to seven β-lactam antibiotics, including ampicillin, methicillin, meropenem, penicillin, aztreonam, amoxicillin, and piperacillin. (frontiersin.org)
  • A. baumannii strains not exposed to the PDAs or antibiotics served as controls. (frontiersin.org)
  • From these perspectives, adjunctive EPI + antibiotics therapeutic strategies may prove to be a promising approach for treating A. baumannii bacteremia. (rochester.edu)
  • Toward those goals we have: A. identified and characterized two novel EPI backbones that potentiate the activity of antibiotics against A. baumannii in a serum-specific manner and B. identified fourteen transposon mutants that exhibit a decrease in serum-specific efflux of antibiotics. (rochester.edu)
  • 4-Hydroxybenzaldehyde (4-HBA), which cannot support the growth of Acinetobacter baumannii , exhibited synergism only with amphenicol antibiotics including chloramphenicol (CAM) and thiamphenicol. (springer.com)
  • Damier-Piolle L, Magnet S, Bremont S, Lambert T, Courvalin P (2008) AdeIJK, a resistance-nodulation-cell division pump effluxing multiple antibiotics in Acinetobacter baumannii . (springer.com)
  • Additionally, chemical scaffolds of DHFR inhibitors that are effective as antibiotics against A. baumannii were identified using an in vitro DHFR activity assay and A. baumannii growth inhibition. (mmv.org)
  • The use of real-time PCR to monitor bacterial growth in the presence of antibiotics is effective for rapidly identifying antibiotic resistance in A. baumannii. (ovid.com)
  • The developing resistance patterns seen in Acinetobacter sp suggest that the number of effective antibiotics may shortly be exhausted. (hud.ac.uk)
  • Ye, Y. Genomic sequencing of a strain of Acinetobacter baumannii and potential mechanisms to antibiotics resistance. (eurekaselect.com)
  • D ) Minimum inhibitory concentration (MIC) of select antibiotics in A. baumannii . (elifesciences.org)
  • Accordingly, our work and that of others showed that A. baumannii senses and responds to extracellular signals, including iron limitation, changes in salt concentrations, desiccation stress, and the presence of antibiotics and disinfectants ( 17 ). (asm.org)
  • While other species of the genus Acinetobacter are often found in soil samples (leading to the common misconception that A. baumannii is a soil organism, too), it is almost exclusively isolated from hospital environments. (wikipedia.org)
  • This means that all the resistances acquired by a strain of A. baumannii can be transmitted to another and the species can thus modify and regenerate itself continuously. (innovations-report.com)
  • In contrast, "non-ACB " Acinetobacter species generally present lower pathogenicity and are often found in the environment. (springer.com)
  • Since it remains unclear how to treat bacteremia caused by AB, the present study aims to enroll patients at multiple sites with extensively drug resistant Acinetobacter species bacteremia receiving different combination therapy to assess treatment outcomes and analyze risk factors associated with mortality. (clinicaltrials.gov)
  • Blood sample will be collected on the day of enrollment (Day 0), Day 1, 2, 3 and 7 for PCR identification and quantification of Acinetobacter species. (clinicaltrials.gov)
  • Acinetobacter baumannii is a non-fermenting Gram-negative bacilli and it is the second most common species after Pseudomonas aeruginosa in this group causing bacterial infections ( Gonzalez-Villoria and Valverde-Garduno, 2016 ). (frontiersin.org)
  • The lice excreted living Acinetobacter species within their feces. (ajtmh.org)
  • Acinetobacter species as a cause of catheter-related infections. (ajtmh.org)
  • Distribution of Acinetobacter species on skin of healthy humans. (ajtmh.org)
  • Determines the citywide prevalence and inter-hospital spread of endemic carbapenem-resistant Acinetobacter species in Brooklyn, New York. (ebscohost.com)
  • The latter subgroup is intrinsic to A. baumannii species 8 ) ( 9 . (scielo.br)
  • Park, W. Antibiotic resistance of pathogenic Acinetobacter species and emerging combination therapy. (eurekaselect.com)
  • Genome sequencing revealed that most were Acinetobacter baumannii , whereas seven represented other Acinetobacter spp. (asm.org)
  • Here, we report a strategic approach with the 3766 proteins from the whole genome of A. baumannii ATCC19606 (AB) to rationally identify plausible. (medworm.com)
  • A bioinformatics analysis showed that the whole genome sequence of phage Βϕ-R3177 exhibited 62% sequence similarity to that of Acinetobacter phage Βϕ-B1252, but there was no homology seen with other phages. (jcvi.org)
  • The circumstances of some outbreaks demonstrated the long survival of Acinetobacter in a dry, inanimate environment. (asm.org)
  • Outbreaks of carbapenem-resistant Acinetobacter baumannii in healthcare facilities have been reported in Europe and worldwide. (medindia.net)
  • Acinetobacter baumannii Gastrointestinal Colonization Is Facilitated by Secretory IgA Which Is Reductively Dissociated by Bacterial Thioredoxin A . mBio, Vol. 9, Issue. (cambridge.org)
  • While the organism is capable of producing an array of bacterial infections, A. baumannii bacteremia is a primary concern, resulting in mortality rates as high as 80% in certain patient populations. (rochester.edu)
  • Nosocomial infections have become alarming with the increase of multidrug resistant bacterial strains of Acinetobacter baumannii. (medworm.com)
  • Our analyses reveal that AA and DHA incorporate into the A. baumannii bacterial membrane and impact bacterial fitness and membrane integrity, with DHA having a more pronounced effect. (asm.org)
  • Through transcriptional profiling and mutant analyses, we show that the A. baumannii β-oxidation pathway plays a protective role against AA and DHA, by limiting their incorporation into the phospholipids of the bacterial membrane. (asm.org)
  • The results demonstrated that silver nanoparticles synthesized with E. critriodora leaf extract triggered MDR A. baumannii DNA condensation, induced bacterial cell death and had a significant effect on biofilm formation, biofilm-grown cells, bacterial attachment and invasion of human lung cells in a concentration dependent manner. (eurekamag.com)
  • We reviewed recent data about epidemiology of Acinetobacter baumannii , resistance mechanisms, and therapeutic options for severe infections caused by multidrug-resistant strains. (medscape.com)
  • Carbapenems may not be considered the treatment of choice in areas with high rates of carbapenem-resistant A. baumannii . (medscape.com)
  • The optimal treatment for multidrug-resistant A. baumannii nosocomial infections has not been established. (medscape.com)
  • Multidrug-resistant A. baumannii has spread to civilian hospitals in part due to the transport of infected soldiers through multiple medical facilities. (wikipedia.org)
  • AbaR-type resistance islands are typical of drug-resistant A. baumannii, and different variations may be present in a given strain. (wikipedia.org)
  • New research finds curcumin combined with a polyphenol in green tea and other foods and herbs will inhibit multi-drug resistant Acinetobacter baumannii - often at the root of hospital-acquried pneumonia infections. (greenmedinfo.com)
  • We describe the prevalence of invasive carbapenem-resistant Acinetobacter spp. (springer.com)
  • Using the Swiss Antibiotic Resistance Centre (anresis) database that includes data from 70% of all hospitalized patients and one third of all ambulatory practitioners in Switzerland, we analysed the number of carbapenem-susceptible and resistant Acinetobacter spp. (springer.com)
  • From 2005 to 2016, 58 cases of resistant or intermediate strains to carbapenem were observed among 632 cases of invasive Acinetobacter . (springer.com)
  • Here we report on the temporal and regional analysis of invasive carbapenem-resistant Acinetobacter isolated in Switzerland from 2005 to 2016. (springer.com)
  • Infections with carbapenem-resistant A. baumannii occur in patients with severe underlying diseases, mainly in intensive care units, and are often related to invasive procedures or indwelling devices. (medindia.net)
  • Recent ECDC data from the European survey on carbapenemase-producing Enterobacteriaceae (EuSCAPE) project and from the European Antimicrobial Resistance Surveillance Network (EARS-Net) confirmed that although there is already a high resistance baseline in some countries, there has been an overall increase of carbapenem-resistant A. baumannii in Europe. (medindia.net)
  • Although there is already a high resistance baseline in some countries, there has been an overall increase of carbapenem-resistant Acinetobacter baumannii in Europe. (medindia.net)
  • A 4-year surveillance of carbapenem-resistant Acinetobacter spp. (asm.org)
  • Multidrug-resistant A . baumannii has emerged as a substantial problem worldwide ( 2 ). (cdc.gov)
  • Unfortunately, multidrug-resistant A . baumannii strains have become endemic in some institutions. (cdc.gov)
  • During the past decade, nosocomial infections due to multidrug-resistant Acinetobacter baumannii have been described with increasing frequency, mostly in intensive care units (ICUs), resulting in therapeutic difficulties ( 1 ). (cdc.gov)
  • Extensively and multi-drug resistant A. baumannii (AB) represent an emerging threat in hospitals locally and worldwide. (clinicaltrials.gov)
  • The Epidemiology of Multidrug-Resistant Acinetobacter Baumannii Does the Community Represent a Reservoir? (cambridge.org)
  • A strain of Acinetobacter baumannii 6B92 isolated from the blood culture of a patient at the Edinburgh Royal Infirmary i 1985 was found to be resistant to imipenem, all classes of cephalosporins and penicillins. (nih.gov)
  • pI 9.0) and a novel beta-lactomase of pI 6.65 named ARI 1 (Acinetobacter resistant to imipenem). (nih.gov)
  • Metal-loaded zeolite remediation of soils contaminated with pandrug-resistant Acinetobacter baumannii. (srce.hr)
  • 2020). 'Metal-loaded zeolite remediation of soils contaminated with pandrug-resistant Acinetobacter baumannii', Arhiv za higijenu rada i toksikologiju , 71(2), str. (srce.hr)
  • Hrenović J, Dekić S, Dikić J, Kazazić S, Durn G, Rajić N. Metal-loaded zeolite remediation of soils contaminated with pandrug-resistant Acinetobacter baumannii. (srce.hr)
  • J. Hrenović, S. Dekić, J. Dikić, S. Kazazić, G. Durn i N. Rajić, "Metal-loaded zeolite remediation of soils contaminated with pandrug-resistant Acinetobacter baumannii", Arhiv za higijenu rada i toksikologiju , vol.71, br. (srce.hr)
  • A prospective 8-month study was conducted to describe the clinical and molecular epidemiology of multidrug-resistant A. baumannii affecting a single hospital. (cambridge.org)
  • Comparative Genomics of Two ST 195 Carbapenem-Resistant Acinetobacter baumannii with Different Susceptibility to Polymyxin Revealed Underlying Resistance Mechanism. (kegg.jp)
  • A. baumannii integrons cassettes carried multi-drug-resistant gene codes. (ebscohost.com)
  • Antibiogram of multidrug resistant Acinetobacter baumannii isolated from clinical specimens at King Hussein Medical Centre, Jordan: a retrospective analysis. (ebscohost.com)
  • In this study, we evaluated the in vitro and in vivo antibacterial activity of DS-8587 against multidrug-resistant (MDR) Acinetobacter baumannii. (sigmaaldrich.com)
  • Among these threats, the rapid increase in carbapenem-resistant Acinetobacter baumannii (CRAB) is a particularly challenging global issue in the health care setting. (jcvi.org)
  • In this study, a novel lytic A. baumannii phage, Βϕ-R3177, infecting carbapenem-resistant A. baumannii strains was isolated from sewage samples at a hospital. (jcvi.org)
  • To determine the occurrence of carbapenem-resistant Acinetobacter baumannii in fish fished in Mediterranean Sea near the Bejaia coast (Algeria), we studied 300 gills and gut samples randomly and prospectively collected during 1 year. (archives-ouvertes.fr)
  • Our best peptide (Ω76) displayed high efficacy against carbapenem and tigecycline-resistant Acinetobacter baumannii in mice. (sciencemag.org)
  • Multi drug resistant (MDR) strains of Acinetobacter baumannii have emerged as a major cause of nosocomial infections associated with significant morbidity and mortality. (hud.ac.uk)
  • Falagas, M.E. Current control and treatment of multidrug-resistant Acinetobacter baumannii infections. (eurekaselect.com)
  • The present study aimed to develop a rational therapy based on the genetic epidemiology, molecular mechanism evaluation and in vitro antibiotic combinations activity in multidrug-resistant Acinetobacter baumannii (MDRAB). (spandidos-publications.com)
  • Conclusion: This study showed a high resistant of A.baumannii to a wide range of antimicrobial agent. (sid.ir)
  • 1% for A. baumannii, and vancomycin-resistant E. faecium were clearly separated from the WT at 6 h. (deepdyve.com)
  • METHODS: One-hundred-and-seven strains of multidrug-resistant Acinetobacter baumannii were isolated from Ruijin Hospital in Shanghai, China. (minervamedica.it)
  • CONCLUSION: Multidrug-resistant Acinetobacter baumannii is highly resistant to aminoglycosides. (minervamedica.it)
  • Between September and November 2009, an outbreak caused by multidrug-resistant Acinetobacter baumannii (MDR-Ab) was observed in the intensive care unit of a tertiary care hospital in Beijing, China. (biomedcentral.com)
  • In the performed study, the infections caused by multi-resistant Acinetobacter baumannii , were observed in COPD, which should be taken into consideration in choosing empirical antibiotic therapy. (aaem.pl)
  • E. Dahdouh, S. H. Shoucair, S. E. Salem, and Z. Daoud, "Mutant Prevention Concentrations of Imipenem and Meropenem against Pseudomonas aeruginosa and Acinetobacter baumannii ," The Scientific World Journal , vol. 2014, Article ID 979648, 7 pages, 2014. (hindawi.com)
  • Carbapenems, including meropenem, are currently considered the first-line agents for the treatment of infections caused by Acinetobacter spp. (asm.org)
  • Septicemia and ventilator-associated pneumonia are two of the more severe forms of A. baumannii disease. (nih.gov)
  • The results revealed that disruption of A. baumannii phospholipase D (PLD) caused a reduction in the organism's ability to thrive in serum, a deficiency in epithelial cell invasion, and diminished pathogenesis in a murine model of pneumonia. (nih.gov)
  • Introduction: Ventilator associated pneumonia and tracheobronchitis (VAP/VAT) due to multiresistant A. baumannii are preeminent causes of mortality and morbidity at ICU's. (ersjournals.com)
  • Although the pneumonia showed radiographic response to the antimicrobial drug therapy, A . baumannii continued to be isolated from respiratory secretions on numerous occasions. (cdc.gov)
  • Community-acquired Acinetobacter pneumonia: a case report. (ajtmh.org)
  • The aim of the study was to analyze pneumonia caused by Acinetobacter baumannii in patients hospitalized because of exacerbation of chronic obstructive pulmonary diseases (COPD), who were admitted to the Pulmonology Ward of the Masovian Specialistic Hospital in Radom (MSS). (aaem.pl)
  • Motility in A. baumannii may also be due to the excretion of exopolysaccharide, creating a film of high-molecular-weight sugar chains behind the bacterium to move forward. (wikipedia.org)
  • These signals affect A. baumannii 's ability to interact with the host, form biofilms on abiotic surfaces, and display motility on semisolid media ( 18 - 21 ). (asm.org)
  • Objective To observe and compare the clinical efficacy of tigecyclinc combined with imipenem/cilastation,imipenem/cilastation combined with cefoperazone sulbacta,prolonging intravenous infusion of imipenem/cilastatin combined with cefoperazone sulbactam on severe pan-resistance Acinetobacter baumannii. (cnki.com.cn)
  • Growth characteristics of A. baumannii mutant library members with increased serum susceptibility. (nih.gov)
  • Antimicrobial Susceptibility Testing Methods for Acinetobacter spp. (apress.com)
  • We report a patient infected with A . baumannii that lacked susceptibility to all commercially available antimicrobial drugs. (cdc.gov)
  • Clonally related strains of Acinetobacter that differ in susceptibility patterns may coexist within a single hospital, dependent on the selective pressure related to antibiotic exposure. (cambridge.org)
  • Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) Effect of Biofield Treatment on Antimicrobials Susceptibility Pattern of Acinetobacter baumannii - An Experimental Study. (omicsonline.org)
  • The aim of the present study was to determine the genetic relatedness and antimicrobial susceptibility patterns in the endemic clones of A. baumannii isolated from patients in the ICU. (magiran.com)
  • Kipnis, A. Acinetobacter baumannii strains isolated from patients in intensive care units in Goiânia, Brazil: Molecular and drug susceptibility profiles. (eurekaselect.com)
  • Rapid disc diffusion antibiotic susceptibility testing for Pseudomonas aeruginosa, Acinetobacter baumannii and Enterococcus spp. (deepdyve.com)
  • Authoritative and cutting-edge, Acinetobacter baumannii: Methods and Protocols aims to ensure successful results in the further study of this high priority area of antibiotic study. (apress.com)
  • There currently exist methods to genetically distinguish A. baumannii strains from each other, but the aim at the moment is to obtain a method of indicating the presence of integrons and their resistance in these strains. (innovations-report.com)
  • The University Hospital and the New Jersey Department of Health are working together to control acinetobacter and are employing all possible methods to control any issue that may arise. (inquisitr.com)
  • Methods: Here, we used CADY, a secondary amphipathic peptide of 20 residues that could successfully carry siRNA into mammalian cells, to prepare CADY/ASOs nanoparticles (CADY-NPs) targeting acpP (encoding acyl carrier protein), and evaluated the uptake features, the inhibitory effects of CADY-NPs on gene expression and the growth of MDR-A. baumannii. (eurekaselect.com)
  • A total of 40 A. baumannii strains, identified using the Phoenix Automated Microbiology System (Becton, Dickinson and Co., Franklin Lakes, NJ, USA) by conventional methods, were included in this study. (medsci.org)
  • Methods In total, 815 clinical strains [E. faecalis (n = 135), E. faecium (n = 227), P. aeruginosa (n = 295) and A. baumannii (n = 158)] were included in this study. (deepdyve.com)
  • The ability of the A. baumannii strains to survive under dry conditions varied greatly and correlated well with the source of the strain. (asm.org)
  • Rapid identification of A. baumannii MOS-1, increased awareness as a result of a French national alert signaling the emergence of ESBL VEB-1-producing A. baumannii ( 5 ), and implementation of strict barrier precautions prevented dissemination of this strain. (cdc.gov)
  • Only the louse strain of A. baumannii was pathogenic for the body louse. (ajtmh.org)
  • We report here the genetic and molecular analysis of the A. baumannii prototype strain 19606 by electroporation of transposon-transposase complexes. (asm.org)
  • This study demonstrates the role of the A. baumannii efflux pump AdeB, and its regulator AdeRS, in multidrug resistance, epithelial cell killing, and biofilm formation. (asm.org)
  • Darwish SA, Rasooli I, Mousavi SG (2017) Filamentous hemagglutinin adhesin FhaB limits A. baumannii biofilm formation. (springer.com)
  • Here, we describe two plasmids carrying mcr-4.3 in two Acinetobacter baumannii strains isolated from imported food and a clinical sample. (asm.org)
  • Survival of Acinetobacter baumannii on dry surfaces. (asm.org)
  • Acinetobacter baumannii is the cause of difficult-to-treat infections in healthcare settings in Europe due to its increasing resistance to antimicrobial agents, in particular the carbapenems. (news-medical.net)
  • A. baumannii that produce PER-1 extended-spectrum β-lactamase (ESBL) are rarely isolated outside Turkey and remain susceptible to carbapenems ( 3 ). (cdc.gov)
  • Rolain, J-M. Emergence of resistance to carbapenems in Acinetobacter baumannii in Europe: clinical impact and therapeutic options. (eurekaselect.com)
  • Risk factors, clinical presentation, and outcome of Acinetobacter barumannii bacteremia. (medigraphic.com)
  • It should be taken into account that the number of hospital patients affected by infections caused by A. baumannii is not great, but the gravity of the problem lies in the rate of mortality of these cases. (innovations-report.com)
  • Once this detailed information is gathered, new systems for the control of infections can be introduced in order keep down the rate of mortality due to A. baumannii. (innovations-report.com)
  • Because of this, and because it is generally found in weakened patients, the mortality rate for infections with A. baumannii is high. (sciencephoto.com)
  • A. baumannii exhibits a wide variety of mechanisms of resistance to antimicrobial agents. (medscape.com)
  • The Food and Drug Administration (FDA) is announcing this public workshop regarding the current state and further development of animal models for serious infections caused by Acinetobacter baumanii and Pseudomonas aeruginosa . (fda.gov)
  • Al-Anazi K, Al-Jasser A. Infections caused by Acinetobacter baumannii in recipients of hematopoietic stem cell transplantation. (medigraphic.com)
  • The main mechanism for resistance to extended-spectrum cephalosporins in A. baumannii is attributed to the overexpression of chromosome-encoded cephalosporinases or to plasmid-encoded Ambler class A, B, and D β-lactamases ( 2 ). (cdc.gov)
  • Indicates wild type A. baumannii containing pMMB plasmid constructs, and cultures grown with the addition of kanamycin (25 μg/mL) to maintain plasmids and 50 μM IPTG for induction. (elifesciences.org)
  • RÉSUMÉ La présente étude a été menée pour déterminer la prévalence et l'antibiogramme local des isolats d'Acinetobacter baumannii multirésistants à l'hôpital Al-Hussein du Centre médical Roi Hussein à Amman (Jordanie). (who.int)
  • Acinetobacter has considerable capacity to spread and develop new antibiotic resistance mechanisms 5 . (scielo.br)
  • Acinetobacter baumannii is a gram-negative aerobic bacillus that primarily causes hospital-acquired infections affecting specially to debilitated patients with prolonged hospitalization and with long-term exposition to antimicrobials. (medscape.com)
  • In this study, we investigated the drug-resistance rates and integrons' distribution in A. baumannii clinical strains in East China. (ebscohost.com)
  • Conclusions: High resistances in A. baumannii clinical strains to most common antimicrobial agents have appeared in East China, which was closely related with high frequencies class 1 integrons. (ebscohost.com)
  • While different basic aspects of the biology and genetics of environmental Acinetobacter strains were analyzed by using transposable elements such as Tn 5 ( 49 ), Tn 10 ( 14 ), Tn 3171 ( 55 ), and mini-Tn 10 PttKm ( 35 ), no work describing the application of this approach to A. baumannii clinical strains could be found. (asm.org)
  • A. baumannii is difficult to eradicate once it has become endemic. (medindia.net)
  • Previous studies have reported the isolation of Acinetobacter baumannii from the body lice of homeless patients. (ajtmh.org)
  • Isolation of Acinetobacter spp. (ajtmh.org)
  • Duplicate entries were removed and only the first date of occurrence of Acinetobacter isolation in case of re-infected patients was kept for a given year. (biomedcentral.com)
  • Al-Agamy MH, Khalaf NG, Tawfick MM, Shibl AM, El Kholy A (2014) Molecular characterization of carbapenem-insensitive Acinetobacter baumannii in Egypt. (springer.com)
  • However, in the case of an outbreak, sources of Acinetobacter must be expected in the dry environment. (asm.org)
  • Clinical microbiologists typically differentiate members of the genus Acinetobacter from other Moraxellaceae by performing an oxidase test, as Acinetobacter spp. (wikipedia.org)
  • Therefore, the A1S_2225 gene, which is present in several members of the Acinetobacter genus, was named b lue- l ight- s ensing A ( blsA ). (asm.org)
  • AF100557 Acinetobacter baumannii DNA gyrase A (gyrA) gene, partial cds. (atcc.org)
  • Bla PER-1 gene in A. baumannii MOS-1 was located on a composite transposon, Tn 1213 , identical to that characterized by Poirel et al. (cdc.gov)
  • We believe that integrons cassettes gene could be taken as a marker of prognosticating A. baumannii antimicrobial resistance, but only reveal partial drug resistance profiles. (ebscohost.com)
  • In addition, the effect of PDAs on the permeability of outer membrane and efflux pumps in A. baumannii was measured. (frontiersin.org)
  • The two PDAs inhibited the function of A. baumannii efflux pump, (AdeABC), but did not increase the permeability of its outer membrane. (frontiersin.org)