Acinetobacter baumannii: A species of gram-negative, aerobic bacteria, commonly found in the clinical laboratory, and frequently resistant to common antibiotics.Acinetobacter Infections: Infections with bacteria of the genus ACINETOBACTER.TennesseeMilk, HumanAnti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Integrated Advanced Information Management Systems: A concept, developed in 1983 under the aegis of and supported by the National Library of Medicine under the name of Integrated Academic Information Management Systems, to provide professionals in academic health sciences centers and health sciences institutions with convenient access to an integrated and comprehensive network of knowledge. It addresses a wide cross-section of users from administrators and faculty to students and clinicians and has applications to planning, clinical and managerial decision-making, teaching, and research. It provides access to various types of clinical, management, educational, etc., databases, as well as to research and bibliographic databases. In August 1992 the name was changed from Integrated Academic Information Management Systems to Integrated Advanced Information Management Systems to reflect use beyond the academic milieu.Microbial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Acinetobacter: A genus of gram-negative bacteria of the family MORAXELLACEAE, found in soil and water and of uncertain pathogenicity.Silver: Silver. An element with the atomic symbol Ag, atomic number 47, and atomic weight 107.87. It is a soft metal that is used medically in surgical instruments, dental prostheses, and alloys. Long-continued use of silver salts can lead to a form of poisoning known as ARGYRIA.Carbapenems: A group of beta-lactam antibiotics in which the sulfur atom in the thiazolidine ring of the penicillin molecule is replaced by a carbon atom. THIENAMYCINS are a subgroup of carbapenems which have a sulfur atom as the first constituent of the side chain.Acinetobacter baumannii: A species of gram-negative, aerobic bacteria, commonly found in the clinical laboratory, and frequently resistant to common antibiotics.Acinetobacter Infections: Infections with bacteria of the genus ACINETOBACTER.Cefoperazone: Semisynthetic broad-spectrum cephalosporin with a tetrazolyl moiety that is resistant to beta-lactamase. It has been proposed especially against Pseudomonas infections.Sulbactam: A beta-lactamase inhibitor with very weak antibacterial action. The compound prevents antibiotic destruction of beta-lactam antibiotics by inhibiting beta-lactamases, thus extending their spectrum activity. Combinations of sulbactam with beta-lactam antibiotics have been used successfully for the therapy of infections caused by organisms resistant to the antibiotic alone.Acinetobacter: A genus of gram-negative bacteria of the family MORAXELLACEAE, found in soil and water and of uncertain pathogenicity.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Carbapenems: A group of beta-lactam antibiotics in which the sulfur atom in the thiazolidine ring of the penicillin molecule is replaced by a carbon atom. THIENAMYCINS are a subgroup of carbapenems which have a sulfur atom as the first constituent of the side chain.Acinetobacter calcoaceticus: A species of gram-negative, aerobic bacteria found in soil and water. Although considered to be normally nonpathogenic, this bacterium is a causative agent of nosocomial infections, particularly in debilitated individuals.Drug Resistance, Multiple, Bacterial: The ability of bacteria to resist or to become tolerant to several structurally and functionally distinct drugs simultaneously. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).Colistin: Cyclic polypeptide antibiotic from Bacillus colistinus. It is composed of Polymyxins E1 and E2 (or Colistins A, B, and C) which act as detergents on cell membranes. Colistin is less toxic than Polymyxin B, but otherwise similar; the methanesulfonate is used orally.Acinetobacter baumannii: A species of gram-negative, aerobic bacteria, commonly found in the clinical laboratory, and frequently resistant to common antibiotics.Acinetobacter Infections: Infections with bacteria of the genus ACINETOBACTER.Acinetobacter: A genus of gram-negative bacteria of the family MORAXELLACEAE, found in soil and water and of uncertain pathogenicity.Carbapenems: A group of beta-lactam antibiotics in which the sulfur atom in the thiazolidine ring of the penicillin molecule is replaced by a carbon atom. THIENAMYCINS are a subgroup of carbapenems which have a sulfur atom as the first constituent of the side chain.Acinetobacter calcoaceticus: A species of gram-negative, aerobic bacteria found in soil and water. Although considered to be normally nonpathogenic, this bacterium is a causative agent of nosocomial infections, particularly in debilitated individuals.Drug Resistance, Multiple, Bacterial: The ability of bacteria to resist or to become tolerant to several structurally and functionally distinct drugs simultaneously. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).Colistin: Cyclic polypeptide antibiotic from Bacillus colistinus. It is composed of Polymyxins E1 and E2 (or Colistins A, B, and C) which act as detergents on cell membranes. Colistin is less toxic than Polymyxin B, but otherwise similar; the methanesulfonate is used orally.Biohazard Release: Uncontrolled release of biological material from its containment. This either threatens to, or does, cause exposure to a biological hazard. Such an incident may occur accidentally or deliberately.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Microbial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Acinetobacter baumannii: A species of gram-negative, aerobic bacteria, commonly found in the clinical laboratory, and frequently resistant to common antibiotics.Acinetobacter Infections: Infections with bacteria of the genus ACINETOBACTER.Acinetobacter: A genus of gram-negative bacteria of the family MORAXELLACEAE, found in soil and water and of uncertain pathogenicity.Carbapenems: A group of beta-lactam antibiotics in which the sulfur atom in the thiazolidine ring of the penicillin molecule is replaced by a carbon atom. THIENAMYCINS are a subgroup of carbapenems which have a sulfur atom as the first constituent of the side chain.Drug Resistance, Multiple, Bacterial: The ability of bacteria to resist or to become tolerant to several structurally and functionally distinct drugs simultaneously. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).Colistin: Cyclic polypeptide antibiotic from Bacillus colistinus. It is composed of Polymyxins E1 and E2 (or Colistins A, B, and C) which act as detergents on cell membranes. Colistin is less toxic than Polymyxin B, but otherwise similar; the methanesulfonate is used orally.Acinetobacter calcoaceticus: A species of gram-negative, aerobic bacteria found in soil and water. Although considered to be normally nonpathogenic, this bacterium is a causative agent of nosocomial infections, particularly in debilitated individuals.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Microbial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Imipenem: Semisynthetic thienamycin that has a wide spectrum of antibacterial activity against gram-negative and gram-positive aerobic and anaerobic bacteria, including many multiresistant strains. It is stable to beta-lactamases. Clinical studies have demonstrated high efficacy in the treatment of infections of various body systems. Its effectiveness is enhanced when it is administered in combination with CILASTATIN, a renal dipeptidase inhibitor.Acinetobacter baumannii: A species of gram-negative, aerobic bacteria, commonly found in the clinical laboratory, and frequently resistant to common antibiotics.Acinetobacter Infections: Infections with bacteria of the genus ACINETOBACTER.Acinetobacter: A genus of gram-negative bacteria of the family MORAXELLACEAE, found in soil and water and of uncertain pathogenicity.Carbapenems: A group of beta-lactam antibiotics in which the sulfur atom in the thiazolidine ring of the penicillin molecule is replaced by a carbon atom. THIENAMYCINS are a subgroup of carbapenems which have a sulfur atom as the first constituent of the side chain.Drug Resistance, Multiple, Bacterial: The ability of bacteria to resist or to become tolerant to several structurally and functionally distinct drugs simultaneously. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).Colistin: Cyclic polypeptide antibiotic from Bacillus colistinus. It is composed of Polymyxins E1 and E2 (or Colistins A, B, and C) which act as detergents on cell membranes. Colistin is less toxic than Polymyxin B, but otherwise similar; the methanesulfonate is used orally.Acinetobacter calcoaceticus: A species of gram-negative, aerobic bacteria found in soil and water. Although considered to be normally nonpathogenic, this bacterium is a causative agent of nosocomial infections, particularly in debilitated individuals.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Microbial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Imipenem: Semisynthetic thienamycin that has a wide spectrum of antibacterial activity against gram-negative and gram-positive aerobic and anaerobic bacteria, including many multiresistant strains. It is stable to beta-lactamases. Clinical studies have demonstrated high efficacy in the treatment of infections of various body systems. Its effectiveness is enhanced when it is administered in combination with CILASTATIN, a renal dipeptidase inhibitor.Pyruvate Dehydrogenase (Lipoamide): The E1 component of the multienzyme PYRUVATE DEHYDROGENASE COMPLEX. It is composed of 2 alpha subunits (pyruvate dehydrogenase E1 alpha subunit) and 2 beta subunits (pyruvate dehydrogenase E1 beta subunit).Dihydrolipoamide Dehydrogenase: A flavoprotein containing oxidoreductase that catalyzes the reduction of lipoamide by NADH to yield dihydrolipoamide and NAD+. The enzyme is a component of several MULTIENZYME COMPLEXES.Pyruvate Dehydrogenase Complex: A multienzyme complex responsible for the formation of ACETYL COENZYME A from pyruvate. The enzyme components are PYRUVATE DEHYDROGENASE (LIPOAMIDE); dihydrolipoamide acetyltransferase; and LIPOAMIDE DEHYDROGENASE. Pyruvate dehydrogenase complex is subject to three types of control: inhibited by acetyl-CoA and NADH; influenced by the energy state of the cell; and inhibited when a specific serine residue in the pyruvate decarboxylase is phosphorylated by ATP. PYRUVATE DEHYDROGENASE (LIPOAMIDE)-PHOSPHATASE catalyzes reactivation of the complex. (From Concise Encyclopedia Biochemistry and Molecular Biology, 3rd ed)Pyruvate Dehydrogenase Complex Deficiency Disease: An inherited metabolic disorder caused by deficient enzyme activity in the PYRUVATE DEHYDROGENASE COMPLEX, resulting in deficiency of acetyl CoA and reduced synthesis of acetylcholine. Two clinical forms are recognized: neonatal and juvenile. The neonatal form is a relatively common cause of lactic acidosis in the first weeks of life and may also feature an erythematous rash. The juvenile form presents with lactic acidosis, alopecia, intermittent ATAXIA; SEIZURES; and an erythematous rash. (From J Inherit Metab Dis 1996;19(4):452-62) Autosomal recessive and X-linked forms are caused by mutations in the genes for the three different enzyme components of this multisubunit pyruvate dehydrogenase complex. One of the mutations at Xp22.2-p22.1 in the gene for the E1 alpha component of the complex leads to LEIGH DISEASE.Acetyl-CoA Carboxylase: A carboxylating enzyme that catalyzes the conversion of ATP, acetyl-CoA, and HCO3- to ADP, orthophosphate, and malonyl-CoA. It is a biotinyl-protein that also catalyzes transcarboxylation. The plant enzyme also carboxylates propanoyl-CoA and butanoyl-CoA (From Enzyme Nomenclature, 1992) EC 6.4.1.2.Acetate-CoA Ligase: An enzyme that catalyzes the formation of CoA derivatives from ATP, acetate, and CoA to form AMP, pyrophosphate, and acetyl CoA. It acts also on propionates and acrylates. EC 6.2.1.1.Carbon-Nitrogen Ligases: Enzymes that catalyze the joining of two molecules by the formation of a carbon-nitrogen bond. EC 6.3.Dihydrolipoyllysine-Residue Acetyltransferase: An enzyme that catalyzes the acetyltransferase reaction using ACETYL CoA as an acetyl donor and dihydrolipoamide as acceptor to produce COENZYME A (CoA) and S-acetyldihydrolipoamide. It forms the (E2) subunit of the PYRUVATE DEHYDROGENASE COMPLEX.Acinetobacter baumannii: A species of gram-negative, aerobic bacteria, commonly found in the clinical laboratory, and frequently resistant to common antibiotics.Fatty Acid Synthase, Type II: The form of fatty acid synthase complex found in BACTERIA; FUNGI; and PLANTS. Catalytic steps are like the animal form but the protein structure is different with dissociated enzymes encoded by separate genes. It is a target of some ANTI-INFECTIVE AGENTS which result in disruption of the CELL MEMBRANE and CELL WALL.Acinetobacter baumannii: A species of gram-negative, aerobic bacteria, commonly found in the clinical laboratory, and frequently resistant to common antibiotics.Acinetobacter Infections: Infections with bacteria of the genus ACINETOBACTER.Acinetobacter: A genus of gram-negative bacteria of the family MORAXELLACEAE, found in soil and water and of uncertain pathogenicity.Carbapenems: A group of beta-lactam antibiotics in which the sulfur atom in the thiazolidine ring of the penicillin molecule is replaced by a carbon atom. THIENAMYCINS are a subgroup of carbapenems which have a sulfur atom as the first constituent of the side chain.Acinetobacter calcoaceticus: A species of gram-negative, aerobic bacteria found in soil and water. Although considered to be normally nonpathogenic, this bacterium is a causative agent of nosocomial infections, particularly in debilitated individuals.Drug Resistance, Multiple, Bacterial: The ability of bacteria to resist or to become tolerant to several structurally and functionally distinct drugs simultaneously. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).Colistin: Cyclic polypeptide antibiotic from Bacillus colistinus. It is composed of Polymyxins E1 and E2 (or Colistins A, B, and C) which act as detergents on cell membranes. Colistin is less toxic than Polymyxin B, but otherwise similar; the methanesulfonate is used orally.UracilAnti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Imipenem: Semisynthetic thienamycin that has a wide spectrum of antibacterial activity against gram-negative and gram-positive aerobic and anaerobic bacteria, including many multiresistant strains. It is stable to beta-lactamases. Clinical studies have demonstrated high efficacy in the treatment of infections of various body systems. Its effectiveness is enhanced when it is administered in combination with CILASTATIN, a renal dipeptidase inhibitor.Colistin: Cyclic polypeptide antibiotic from Bacillus colistinus. It is composed of Polymyxins E1 and E2 (or Colistins A, B, and C) which act as detergents on cell membranes. Colistin is less toxic than Polymyxin B, but otherwise similar; the methanesulfonate is used orally.TracheitisAcinetobacter baumannii: A species of gram-negative, aerobic bacteria, commonly found in the clinical laboratory, and frequently resistant to common antibiotics.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Acinetobacter Infections: Infections with bacteria of the genus ACINETOBACTER.Intensive Care Units: Hospital units providing continuous surveillance and care to acutely ill patients.APACHE: An acronym for Acute Physiology and Chronic Health Evaluation, a scoring system using routinely collected data and providing an accurate, objective description for a broad range of intensive care unit admissions, measuring severity of illness in critically ill patients.Polymyxins: Basic lipopeptide antibiotic group obtained from Bacillus polymyxa. They affect the cell membrane by detergent action and may cause neuromuscular and kidney damage. At least eleven different members of the polymyxin group have been identified, each designated by a letter.Bronchitis: Inflammation of the large airways in the lung including any part of the BRONCHI, from the PRIMARY BRONCHI to the TERTIARY BRONCHI.Polymyxin B: A mixture of polymyxins B1 and B2, obtained from Bacillus polymyxa strains. They are basic polypeptides of about eight amino acids and have cationic detergent action on cell membranes. Polymyxin B is used for infections with gram-negative organisms, but may be neurotoxic and nephrotoxic.Pentose Phosphate Pathway: An oxidative decarboxylation process that converts GLUCOSE-6-PHOSPHATE to D-ribose-5-phosphate via 6-phosphogluconate. The pentose product is used in the biosynthesis of NUCLEIC ACIDS. The generated energy is stored in the form of NADPH. This pathway is prominent in tissues which are active in the synthesis of FATTY ACIDS and STEROIDS.Acinetobacter baumannii: A species of gram-negative, aerobic bacteria, commonly found in the clinical laboratory, and frequently resistant to common antibiotics.PentosephosphatesPhosphoribosyl Pyrophosphate: The key substance in the biosynthesis of histidine, tryptophan, and purine and pyrimidine nucleotides.Transaldolase: An enzyme of the transferase class that catalyzes the reaction sedoheptulose 7-phosphate and D-glyceraldehyde 3-phosphate to yield D-erythrose 4-phosphate and D-fructose phosphate in the PENTOSE PHOSPHATE PATHWAY. (Dorland, 27th ed) EC 2.2.1.2.Acinetobacter Infections: Infections with bacteria of the genus ACINETOBACTER.Ribose-Phosphate Pyrophosphokinase: An enzyme that catalyzes the formation of phosphoribosyl pyrophosphate from ATP and ribose-5-phosphate. EC 2.7.6.1.Ribosemonophosphates: Ribose substituted in the 1-, 3-, or 5-position by a phosphoric acid moiety.Phosphogluconate Dehydrogenase: An enzyme of the oxidoreductase class that catalyzes the reaction 6-phospho-D-gluconate and NADP+ to yield D-ribulose 5-phosphate, carbon dioxide, and NADPH. The reaction is a step in the pentose phosphate pathway of glucose metabolism. (From Dorland, 27th ed) EC 1.1.1.43.Glucosephosphate Dehydrogenase
(1/853) Distribution of beta-lactamases in Acinetobacter baumannii clinical isolates and the effect of Syn 2190 (AmpC inhibitor) on the MICs of different beta-lactam antibiotics.

The distribution of beta-lactamases in a group of 20 epidemiologically well defined Acinetobacter baumannii clinical isolates and the in vitro activity of Syn 2190, a novel beta-lactamase AmpC inhibitor, were determined. Twenty-five per cent of the strains carried and expressed a TEM-type beta-lactamase, whereas 35% had an OXA-type beta-lactamase. In nine out of 11 (82%) ceftazidime-resistant and four out of 13 (30.7%) cefepime-resistant strains, the MIC of these beta-lactam antibiotics decreased when determined in the presence of Syn 2190. Thus, our results suggest that in a high percentage of A. baumannii clinical isolates the increased production of AmpC, in combination or not with other resistance mechanisms, contributes to the resistance pattern in A. baumannii to beta-lactams.  (+info)

(2/853) Molecular characterization of integrons in epidemiologically unrelated clinical isolates of Acinetobacter baumannii from Italian hospitals reveals a limited diversity of gene cassette arrays.

Integron carriage by 36 epidemiologically unrelated Acinetobacter baumannii isolates collected over an 11-year period from patients in six different Italian hospitals was investigated. Sixteen type 1 integron-positive isolates (44%) were found, 13 of which carried the same array of cassettes, i.e., aacC1, orfX, orfX', and aadA1a. As ribotype analysis of the isolates demonstrated a notable genetic diversity, horizontal transfer of the entire integron structure or ancient acquisition was hypothesized.  (+info)

(3/853) Genetic and phenotypic analysis of Acinetobacter baumannii insertion derivatives generated with a transposome system.

Acinetobacter baumannii is a metabolically versatile pathogen that causes severe infections in compromised patients. However, little is known about the genes and factors involved in its basic physiology and virulence properties. Insertion mutagenesis was used to initiate the identification and characterization of some of these factors and genes in the prototype strain 19606. The utilization of the pLOFKm suicide delivery vector, which harbors a suicide mini-Tn10 derivative, proved to be unsuccessful for this purpose. The EZ::TN Tnp transposome system available from Epicentre was then used in conjunction with electroporation to generate isogenic insertional derivatives of A. baumannii 19606. Replica plating showed that 2% of the colonies that grew after electroporation on agar plates without antibiotics also grew in the presence of 40 micro g of kanamycin per ml. DNA hybridization proved that all of the kanamycin-resistant derivatives contained the EZ::TN insertion element, which was mapped to different genomic locations. Replica plating on Simmons citrate agar and microtiter plate-plastic tube assays identified growth- and biofilm-defective derivatives, respectively. The location of the insertion in several of these derivatives was determined by self-ligation of NdeI- or EcoRI-digested genomic DNA and electroporation of Escherichia coli TransforMax EC100D (pir(+)). Sequence analysis of the recovered plasmids showed that some of the A. baumannii 19606 growth-defective derivatives contain insertions within genes encoding activities required for the generation of energy and cell wall components and for the biosynthesis of amino acids and purines. A gene encoding a protein similar to the GacS sensor kinase was interrupted in four derivatives, while another had an insertion in a gene coding for a hypothetical sensor kinase. A. baumannii 19606 derivatives with defective attachment or biofilm phenotypes had insertions within genes that appear to be part of a chaperone-usher transport system described for other bacteria. DNA hybridization experiments showed that the presence of strain 19606 genes encoding regulatory and attachment or biofilm functions is widespread among other A. baumannii clinical isolates.  (+info)

(4/853) Comparison of a repetitive extragenic palindromic sequence-based PCR method and clinical and microbiological methods for determining strain sources in cases of nosocomial Acinetobacter baumannii bacteremia.

Using a repetitive extragenic palindromic PCR (REP-PCR), we genotypically characterized strains causing nosocomial Acinetobacter baumannii infections and analyzed the source of bacteremia in 67 patients from an institution in which infections by this bacterium were endemic. Six different genotypes were found, including 21, 27, 3, 9, 3, and 4 strains. The probable source of bacteremia, according to clinical and/or microbiological criteria, was known in 42 patients (63%): respiratory tract (n = 19), surgical sites (n = 12), intravascular catheters (n = 5), burns (n = 3), and urinary tract (n = 3). The definite source of bacteremia, according to REP-PCR, could be established in 30 (71%) out of the 42 patients with strains from blood and other sites; in these cases clinical and microbiological criteria for the source of bacteremia were thus confirmed. In the remaining 12 patients (29%) the probable source was refuted by the REP-PCR method. The definite sources of bacteremia according to genotype were as follows: respiratory tract in 13 patients (31%), surgical sites in 8 (19%), intravascular catheters in 4 (9%), burns in 3 (7%), and urinary tract in 2 (5%). A comparison of strains from blood cultures and other sites with regard to their REP-PCR and antimicrobial resistance profiles was also made. Taking the REP-PCR as the "gold standard," the positive predictive value of antibiotype was 77% and the negative predictive value was 42%. In summary, the utility of the diagnosis of the source of nosocomial A. baumannii bacteremia using clinical and/or microbiological criteria, including antibiotyping, is limited, as demonstrated by REP-PCR.  (+info)

(5/853) Endemic carbapenem resistance associated with OXA-40 carbapenemase among Acinetobacter baumannii isolates from a hospital in northern Spain.

Eighty-two carbapenem-resistant isolates of Acinetobacter baumannii from a single hospital in Bilbao were typed into two major clusters and several subclusters. Disk synergy tests and PCR indicated the production of a zinc-independent OXA-class carbapenemase. Sequencing identified this enzyme, OXA-40, as a variant of the OXA-24-OXA-25-OXA-26 cluster.  (+info)

(6/853) Loss of a 29-kilodalton outer membrane protein in Acinetobacter baumannii is associated with imipenem resistance.

We analyzed the possible causes of imipenem (IPM) resistance in multidrug-resistant isolates of Acinetobacter baumannii. Comparison of the outer membrane protein (OMP) profiles of two genomically related strains (Ab288 [IPM sensitive] and Ab242 [IPM resistant]) indicated the conspicuous loss of a 29-kDa polypeptide in the Ab242 strain. No carbapenemase activity was detected in any of these strains. The treatment of Ab288 with sodium salicylate resulted in IPM resistance and the loss of the 29-kDa OMP. In addition, IPM-resistant clones of Ab288 which were selected by repetitive culturing in increasing concentrations of this antibiotic also showed the absence of this 29-kDa OMP.  (+info)

(7/853) Genetic and functional analysis of the chromosome-encoded carbapenem-hydrolyzing oxacillinase OXA-40 of Acinetobacter baumannii.

Clinical isolate Acinetobacter baumannii CLA-1 was resistant to a series of antibiotic molecules, including carbapenems. Cloning and expression of the beta-lactamase gene content of this isolate in Escherichia coli DH10B identified a chromosome-encoded oxacillinase, OXA-40, that differed by one or two amino acid changes from OXA-24, -25, and -26 and an AmpC-type cephalosporinase. The OXA-40 beta-lactamase had a mainly narrow-spectrum hydrolytic profile, but it included ceftazidime and imipenem. Its activity was resistant to inhibition by clavulanic acid, tazobactam, sulbactam, and, like most of the other carbapenem-hydrolyzing oxacillinases, NaCl. OXA-40 had an FGN triad replacing a YGN motif at class D beta-lactamase (DBL) positions 144 to 146. Site-directed DNA mutagenesis leading to a Phe-to-Tyr change at DBL position 144 in OXA-40 gave a mutant enzyme with increased hydrolytic activity against most beta-lactams, including imipenem. Conversely, with a gene encoding the narrow-spectrum oxacillinase OXA-1 as the template, a nucleotide substitution leading to a Tyr-to-Phe change in the YGN motif of OXA-1 gave a mutant enzyme with decreased hydrolytic activity without an increase in carbapenem-hydrolyzing activity. Thus, the Phe residue in the FGN motif was not associated with carbapenem-hydrolyzing activity by itself but instead was associated with weak overall hydrolytic activity. Finally, this Phe residue in OXA-40 explained resistance to inhibition by NaCl whereas a Tyr residue in motif YGN was related to susceptibility to NaCl.  (+info)

(8/853) Relationship between beta-lactamase production, outer membrane protein and penicillin-binding protein profiles on the activity of carbapenems against clinical isolates of Acinetobacter baumannii.

Twenty blood isolates of Acinetobacter baumannii were studied, representing eight pulsed-field gel electrophoresis patterns and all different antimicrobial susceptibility patterns observed during 1995-97 at the University Hospital Virgen Macarena, Seville, Spain. The MIC(90)s (mg/L) of imipenem and meropenem decreased from 16 to 0.5 and from 8 to 4, respectively, in the presence of BRL 42715 (BRL) but not clavulanic acid. Hydrolysing activity (nmol/min/mg) of bacterial supernatants against cefaloridine ranged from 8.8 to 552.3 for A. baumannii type I (imipenem MICs < or = 2), which expressed only a beta-lactamase of pI > or = 9, and from 12.3 to 1543.5 for A. baumannii type II (imipenem MICs > or = 4), which expressed a beta-lactamase of pI > or = 9 and two others of pI 6.3 and 7. The hydrolysing activities of A. baumannii type II against imipenem, meropenem and oxacillin were higher than those observed for A. baumannii type I. Ten outer membrane protein (OMP) profiles (A. baumannii types I and II) were visualized on 10% SDS-PAGE gels with 6 M urea, whereas only five OMP profiles (A. baumannii types I and II) were differentiated in 12% SDS-PAGE gels. Five A. baumannii with OMP profile type B, characterized by the absence of a 22.5 kDa OMP, were resistant to meropenem and/or imipenem. Twelve penicillin-binding protein (PBP) patterns were observed. PBP patterns of A. baumannii type II were characterized by the absence of a 73.2 kDa band (PBP 2). We concluded that production of beta-lactamases of pI 6.3 and 7.0 and reduced expression of PBP 2 are the most frequently observed mechanisms of resistance to carbapenems. In some isolates, loss of a 22.5 kDa OMP is also related to resistance to carbapenems.  (+info)

*  Acinetobacter calcoaceticus
Together with A. baumannii, it is referred to as the A. calcoaceticus-A. baumannii complex, which is relatively simple to ... Acinetobacter calcoaceticus is a bacterial species of the genus Acinetobacter. It is a nonmotile, gram negative coccobacillus. ... Acinetobacter calcoaceticus on www.ncbi.nlm.nih.gov Type strain of Acinetobacter calcoaceticus at BacDive - the Bacterial ... baumannii complex. Other contributing factors include the cost-effective nature of A. calcoaceticus compared to A. baumannii ...
*  Acinetobacter
... baumannii can be identified by OXA-51 typing) Acinetobacter lwoffii: glucose-negative nonhemolytic Acinetobacter haemolyticus: ... in particular the species Acinetobacter baumannii. Species of the genus Acinetobacter are strictly aerobic, nonfermentative, ... Of the Acinetobacter, A. baumannii is the greatest cause of human disease, having been implicated in a number of hospital- ... Antibiotic resistance is a major risk factor for epidemic behavior of Acinetobacter baumannii. Infect Control Hosp Epidemiol ...
*  Acinetobacter junii
"Taxonomy of the Genus Acinetobacter with the Recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. ... nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov. and Emended Descriptions of Acinetobacter ... Acinetobacter junii at the Encyclopedia of Life Type strain of Acinetobacter junii at BacDive - the Bacterial Diversity ... "Reclassification of Acinetobacter grimontii Carr et al. 2003 as a later synonym of Acinetobacter junii Bouvet and Grimont 1986 ...
*  Drug of last resort
Used to treat XDR Acinetobacter baumannii infections. Tolcapone - used in patients with Parkinson's disease who are not ...
*  Waxworm
"Deciphering the Multifactorial Nature of Acinetobacter baumannii Pathogenicity". PLoS ONE. 6 (8): e22674. doi:10.1371/journal. ...
*  Plazomicin
It also demonstrates potent in vitro activity versus carbapenem-resistant Acinetobacter baumannii. In 2012, U.S. Food and Drug ... "Can Plazomicin Alone or in Combination Be a Therapeutic Option against Carbapenem-Resistant Acinetobacter baumannii?" (PDF). ...
*  List of antibiotic resistant bacteria
"Medscape abstract on Acinetobacter baumannii: Acinetobacter baumannii: An Emerging Multidrug-resistant Threat". membership only ... Centers for Disease Control Prevention (CDC). (2004). "Acinetobacter baumannii infections among patients at military medical ... reported an increasing number of Acinetobacter baumannii bloodstream infections in patients at military medical facilities in ... Acinetobacter is a gram-negative bacteria that causes pneumonia or bloodstream infections in critically ill patients. Multidrug ...
*  Colistin
Use of colistin to treat Acinetobacter baumannii infections has led to the development of resistant bacterial strains. which ... Colistimethate sodium has also been given intrathecally and intraventricularly in Acinetobacter baumannii and Pseudomonas ... Towner K J (2008). "Molecular Basis of Antibiotic Resistance in Acinetobacter spp.". Acinetobacter Molecular Biology. www. ... "In-vitro activity of the combination of colistin and rifampicin against multidrug-resistant strains of Acinetobacter baumannii ...
*  Ventriculitis
Successful Treatment of Multidrug-Resistant Acinetobacter baumannii Ventriculitis with Intravenous and Intraventricular ...
*  Phage therapy
IV phage drip therapy was successfully used to treat a patient with MDR Acinetobacter baumannii in Thornton Hospital at UC San ... "Bacteriophage therapy treats patient near death with MDR Acinetobacter baumannii - Outbreak News Today". 25 April 2017. Keen, E ...
*  Resistance-nodulation-cell division superfamily
"Overexpression of resistance-nodulation-cell division pump AdeFGH confers multidrug resistance in Acinetobacter baumannii." ...
*  Norwogonin
"Isolation and Characterization of Antimicrobial Compounds in Plant Extracts against Multidrug-Resistant Acinetobacter baumannii ...
*  Acinetobacter seifertii
nov., a member of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex isolated from human clinical specimens". ... Parte, A.C. "Acinetobacter". www.bacterio.net. "Acinetobacter seifertii". www.uniprot.org. Nemec, A; Krizova, L; Maixnerova, M ... Acinetobacter seifertii is bacterium from the genus Acinetobacter which has been isolated from human clinical specimens. ... Sedo, O; Brisse, S; Higgins, PG (March 2015). "Acinetobacter seifertii sp. ...
*  Eravacycline
"Activity of eravacycline against Enterobacteriaceae and Acinetobacter baumannii, including multidrug-resistant isolates, from ... Non-lactose fermenting Gram-negative bacteria Acinetobacter baumannii Stenotrophomonas maltophilia Haemophilus influenzae ...
*  Carbapenem
"Challenges in identifying new antimicrobial agents effective for treating infections with Acinetobacter baumannii and ... It lacks useful activity against the P. aeruginosa and Acinetobacter species, both of which are important causes of hospital- ... Antibiotics cross the outer membrane of Pseudomonas and Acinetobacter approximately 100 times more slowly than they cross the ... Infections caused by the non-fermenting gram-negative bacteria Pseudomonas aeruginosa and Acinetobacter baumanni are most ...
*  New Delhi metallo-beta-lactamase 1
In July 2010, a team in New Delhi reported a cluster of three cases of Acinetobacter baumannii bearing blaNDM-1 that were found ... "Coexistence of blaOXA-23 with blaNDM-1 and armA in clinical isolates of Acinetobacter baumannii from India". J Antimicrob ... The patient was also found to be carrying an Acinetobacter strain. The patient contracted the bacteria after another patient, ...
*  Bacteriophage
... outbreaknewstoday.com/bacteriophage-therapy-treats-patient-near-death-mdr-acinetobacter-baumannii-45488/ Wommack, K. E.; ...
*  Mark Pallen
2011). "Whole-genome comparison of two Acinetobacter baumannii isolates from a single patient, where resistance developed ... 2014). "Genomic epidemiology of a protracted hospital outbreak caused by multidrug-resistant Acinetobacter baumannii in ...
*  Minocycline
... and Acinetobacter baumannii". Int. J. Antimicrob. Agents. 34 (5): 395-401. doi:10.1016/j.ijantimicag.2009.06.021. PMID 19665876 ... It may be used to treat certain strains of MRSA infection and a disease caused by drug resistant Acinetobacter. Both ...
*  Diaminobutyrate decarboxylase
4-diaminobutyrate decarboxylase of Acinetobacter baumannii". FEMS Microbiol. Lett. 124 (2): 225-8. doi:10.1111/j.1574-6968.1994 ... 3-diaminopropane production pathway in Acinetobacter baumannii". J. Bacteriol. 179 (16): 5118-25. PMC 179370 . PMID 9260954. ... 4-diaminobutyrate decarboxylase from Acinetobacter calcoaceticus". J. Gen. Microbiol. 138 (7): 1461-5. doi:10.1099/00221287-138 ...
*  Antimicrobial copper-alloy touch surfaces
Acinetobacter baumannii was isolated from a patient in a burn unit and two clinical strains of MTB were collected and tested. ... Acinetobacter baumanii, Enterococcus spp., and Candida albicans on the copper alloy surfaces versus the non-copper standard ...
*  Tigecycline
Acinetobacter baumannii, and E. coli. As a tetracycline derivative antibiotic, its structural modifications has expanded its ... and multi-drug resistant strains of Acinetobacter baumannii. It has no activity against Pseudomonas spp. or Proteus spp. The ...
*  Gram-negative bacteria
... associated with hospital-acquired infections include Acinetobacter baumannii, which cause bacteremia, ... It has also been studied in gram-negative species found in soil such as Pseudomonas stutzeri, Acinetobacter baylyi, and gram- ...
*  Diaminobutyrate-2-oxoglutarate transaminase
3-diaminopropane production pathway in Acinetobacter baumannii". J. Bacteriol. 179 (16): 5118-25. PMC 179370 . PMID 9260954. ...
*  Multidrug-resistant Gram-negative bacteria
MDR strains of Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii have become of most concern because they ... Acinetobacter baumannii, Klebsiella pneumoniae and Stenotrophomonas maltophilia. Also, there has been interest in the drug ...
*  Michael P. Snyder
Smith MG, Gianoulis TA, Pukatzki S, Mekalanos J, Ornston LN, Gerstein M, Snyder M. New insights into Acinetobacter baumannii ... They sequenced Acinetobacter Baummanii, a human pathogen with low error rates (19). They invented paired end sequencing using ...
*  Imipenem
... while Acinetobacter baumannii, some Acinetobacter spp., Bacteroides fragilis, and Enterococcus faecalis have developed ... Acinetobacter anitratus, Acinetobacter calcoaceticus, Actinomyces odontolyticus, Aeromonas hydrophila, Bacteroides distasonis, ...
Objectives. Multidrug-resistant Acinetobacter strain HK302 was isolated from an outbreak of nosocomial infections in Switzerland in 1977. The aim of the present study was to assess whether this archive strain belongs to one of the known international clonal lineages of Acinetobacter baumannii and whether it harbours a genomic structure related to the AbaR1-like resistance islands.. Methods. Multilocus sequence typing (MLST) and HindIII ribotyping were used to determine the taxonomic position of HK302 at the species and subspecies (clonal) levels. The position and structure of the putative resistance island were investigated by AbaR1-based PCR mapping followed by restriction analysis and partial sequencing of amplicons. A. baumannii AYE harbouring AbaR1 was used as a positive control for PCR mapping.. Results. The MLST allelic profile (1-1-1-1-5-1-1) and HindIII ribotype of HK302 were typical of A. baumannii European (EU) clone I. In addition, an AbaR1-related region inserted into the ATPase gene ...
Dr. Peymani, Amir (2013) Prevalence of PER and VEB Type Extended Spectrum Betalactamases among Multidrug Resistant Acinetobacter baumannii Isolates in North-West of Iran. [Teaching Resource] ...
Opazo , A , Lopes , B S , García , P , Domínguez Yévenes , M , Lima , C , Bello-Toledo , H , González-Rocha , G & Amyes , S G B 2015 , Draft Genome Sequence of a Multidrug-Resistant Acinetobacter baumannii Strain from Chile Genome Announcements , vol 3 , no. 4 , e00687-15 . DOI: 10.1128/genomeA.00687- ...
Acinetobacter baumannii ATCC ® BAA-1710D-5™ Designation: Genomic DNA from Acinetobacter baumannii strain AYE (ATCC ® BAA-1710™) TypeStrain=False Application:
Nosocomial pathogens can be associated with a variety of infections, particularly in intensive care units (ICUs) and in immunocompromised patients. Usually these pathogens are resistant to multiple drugs and pose therapeutic challenges. Among these organisms, Acinetobacter baumannii is one of the most frequent being encountered in the clinical setting. Carbapenems are very useful to treat infections caused by these drug-resistant Gram-negative bacilli, but carbapenem resistance is increasing globally. Combination therapy is frequently given empirically for hospital-acquired infections in critically ill patients and is usually composed of an adequate beta-lactam and an aminoglycoside. The purpose of this study was to evaluate the in vitro activity of plazomicin against carbapenem-resistant Acinetobacter baumannii. Amikacin was used as a comparator. The activity of plazomicin in combination with several different antibiotics was tested by disk diffusion, the checkerboard method, and time-kill ...
The worldwide dissemination of multi drug resistant Acinetobacter baumannii strains has caused serious concern and high rate of mortality in recent decades that originate from limited effective antibiotics in the treatment of A. baumannii infections. Myxobacteria are Gram-negative bacteria that are important for their complex lifestyle and production of novel structurally secondary metabolites with diverse bioactivities. In this study, a total of 60 myxobacterial strains were purified by culturing and investigation of 130 soil samples. Secondary metabolite extracts of the selected strains were screened for antibacterial activity against multi-drug resistant (MDR) Acinetobacter baumannii. The most potent extracts derived from Stigmatella sp. UTMC 4081, Stigmatella sp. UTMC 4072, and Archangium sp. UTMC 4070 which were investigated by recording percentage of growth inhibition, MIC, MBC, and IC50 values. The results showed that the MIC value of extract No. 4072 was 2.5 μg/ml and its MBC value against A.
BioAssay record AID 582088 submitted by ChEMBL: Effect on morphological changes in colistin-susceptible Acinetobacter baumannii ATCC 19606 assessed as change in length of cellular dimension at mid-log phase at 4 ug/ml after 20 mins by atomic force microscopic analysis.
Multidrug-resistant Acinetobacter baumannii infection has presented a global medical challenge. The antibiograms of paired colistin-susceptible and -resistant strains revealed increased susceptibility of colistin-resistant strains to most tested antibiotics, including those that are active against only gram-positive bacteria. Synergy between colistin and rifampicin was observed in the colistin-susceptible strains. The ability to form biofilm in the colistin-resistant strains was significantly lower (P , .001) than in the parent strains. Our study provides valuable information for potential expansion of our current therapeutic options against colistin-resistant A. baumannii infection.. ...
Carbapenem resistance in A. baumannii is most often associated with class D β-lactamases (OXA-23-like, OXA-40-like and OXA-58-like) and MBLs. OXA-type carbapenemases are predominant in A. baumannii, particularly in worldwide outbreaks of OXA-23 [24]. The molecular analysis of the isolates tested in this study revealed that 14 strains (51.8 %) carried the blaOXA-23-like gene and that two strains carried a blaOXA-24-like gene. All of the strains had a blaOXA-51-like gene, and four strains had a blaOXA-58 gene. In this study, the OXA-58 isolates presented lower MIC values for meropenem than OXA-23-like-positive isolates, which systematically exhibited higher MIC values (Table 1). The isolates with non-acquired OXA genes displayed a marked variation and included some carbapenem-resistant genes. Naturally occurring OXA carbapenemases, such as OXA-51-like enzymes (e.g., OXA 64-66, OXA 68-71, OXA 78-80, OXA-82, OXA-86, OXA-92 and OXA104-112), have been identified in A. baumannii isolates worldwide. In ...
Acinetobacter baumannii has been increasingly reported in the outbreak of nosocomial infections in the intensive care units, which not only prolong the length of hospital stay but result in high attributable mortality. With its intrinsic resistance to many antimicrobial agents and rapid acquirement of resistance mechanism, resistance to carbapenems, which is often accompanied with resistance to multiple drugs, has emerged worldwide. The limited treatment choice included tigecycline, colistin, and sulbactam. However, the low serum level and bacteriostatic nature of tigecycline hamper its application in blood stream infection, one of the most common presentations of A. baumannii infections. The nephrotoxicity and neurotoxicity of intravenous colistin have caused great concerns in critically ill patients whereas immediate bronchospasm after inhalation and significant clinical consequences have been reported. Sulbactam has been used for decades in combination of ampicillin and well tolerated. ...
Acinetobacter baumannii ATCC ® 43498™ Designation: S2 TypeStrain=False Application: Assay of cefoperazone Assay of sulbactam Quality control of cefoperazone/sulbactam
In February 2006, a patient colonized with a multidrug-resistant sequence type 56 Acinetobacter baumannii strain was admitted to a hospital in Madrid, Spain. This strain spread rapidly and caused a large outbreak in the hospital. Clinicians should be ...
BioAssay record AID 586058 submitted by ChEMBL: Antimicrobial activity against Acinetobacter baumannii clinical isolate harboring chromosomally encoded ISAba1-blaOXA-51-like gene with fxsA upstream sequence by agar dilution method.
Acinetobacter baumannii causes severe nosocomial infections such as pneumonia, meningitis and sepsis with high mortality rates. This organism represents an increasing danger for immunocompromised adults, especially since there are an increasing number of resistances against antibiotics. Until now, scientific investigation was mainly focused on taxonomy and antibiotic resistance mechanisms. The goal of this project was to analyse the interaction between clinical strains of Acinetobacter baumannii and human cells in order to address the molecular mechanisms causing pathogenicity. Adherence is the first step in colonization of human tissue, and therefore a key event in pathogenesis. To demonstrate the adhesion of bacteria to human cells, a colony counting assay has been established. These experiments used the the type strain of A. baumannii ATCC 19606, as well as clinical isolates. All A. baumannii strains investigated showed adhesion to the lung epithelial cells A549, but the adhesion capacity was ...
Acinetobacter baumannii is currently one of the key nosocomial pathogens causing severe infections; of special concern is its resistance to expanded-spectrum cephalosporins (ESCs) and carbapenems, often associated with the few so-called European clones (6, 7, 19). It has two natural -lactamases, an AmpC-like enzyme (Acinetobacter-derived cephalosporinase [ADC]) (10) and a carbapenem-hydrolyzing class D -lactamase (CHDL; the OXA-51 type) (15), which affect susceptibility upon increased expression due to ISAba1 insertion upstream of their genes (9, 18). Moreover, acquired -lactamases, including metallo-lactamases (MBLs) and four CHDL types, the OXA-23, OXA-24/40, OXA-58, and OXA-143 types, are observed (15). Knowledge of A. baumannii in Poland has been limited to single isolates (9, 14, 21); our aim was to analyze a bigger group of A. baumannii strains. (Part of this work was presented at the 22nd European Congress of Clinical Microbiology and Infectious Diseases, London, United Kingdom, 31 March to 3
To understand the epidemiology of multidrug-resistant (MDR) Acinetobacter baumannii and define individual risk factors for MDR, we used epidemiologic methods, performed organism typing by pulsed-field gel electrophoresis (PFGE), and conducted a matched case-control retrospective study. We investigated 118 patients, on 27 wards, in whom MDR A. baumannii was isolated from clinical cultures. Each case-patient had a control without MDR A. baumannii and was matched for hospital length of stay, ward, and calendar time. The epidemiologic investigation found small clusters of up to 6 patients each with no common identified source. Ten different PFGE clones were found, of which 2 dominated. The PFGE pattern differed within temporospatial clusters, and antimicrobial drug susceptibility patterns varied within and between clones. Multivariate analysis identified the following significant risk factors: male sex, cardiovascular disease, having undergone mechanical ventilation, and having been treated with
Hospital-acquired infections due to Acinetobacter baumannii have become problematic because of high rates of drug resistance. A. baumannii is usually harmless, but it causes sepsis resulting in a high mortality rate in compromised hosts. Therefore, we must consider its interaction with host cells to understand diseases resulting from A. baumannii infection. Neutrophils play a critical role in infective protection against the extracellular growth of bacteria. However, their interactions with A. baumannii remain largely unknown. Recently, a new biological defense mechanism called neutrophil extracellular traps (NETs) has been attracting attention. In the present study, we investigated the responsiveness of human neutrophils to A. baumannii focusing on NET formation. The results demonstrated that infective protection against Pseudomonas aeruginosa via NETs formation was observed, but for A. baumannii NETs formation did not occur. It seems that the innate infective protection against A. baumannii ...
The use of bacteriophages offers an appealing alternative to antibiotics for the control of pathogenic bacteria. Recently, bacteriophage AbauYa1 was isolated as part of an effort to find phages to combat Acinetobacter baumannii infections. In addition to causing lysis of the host cell, AbauYa1 depolymerizes the polysaccharides of the A. baumannii capsules. The A. baumannii capsule is an important virulence factor, and phage depolymerases have been shown to disrupt biofilms of other pathogenic bacterial strains. The gene encoding the protein responsible for this activity was cloned, and the protein was expressed, purified, and enzymatically assayed. The protein was found to degrade the polysaccharide capsule, as shown by an increase in reducing ends upon incubating the capsule with the depolymerase. Finally, the protein removes A. baumannii biofilms from a polystyrene surface. The protein degrades A. baumannii capsule and biofilms and therefore carries high therapeutic potential for treating A. ...
Sigma-Aldrich offers abstracts and full-text articles by [Danielle M Stacy, Michael A Welsh, Philip N Rather, Helen E Blackwell].
Introduction: Acinetobacter baumannii is opportunistic in debilitated hospitalised patients. Because information from some South American countries was previously lacking, this study examined the emergence of multi-resistant A. baumannii in three hospitals in Cochabamba, Bolivia, from 2008 to 2009. Methodology: Multiplex PCR was used to identify the main resistance genes in 15 multi-resistant A. baumannii isolates. RT-PCR was used to measure gene expression. The genetic environment of these genes was also analysed by PCR amplification and sequencing. Minimum inhibitory concentrations were determined for key antibiotics and some were determined in the presence of an efflux pump inhibitor, 1-(1-napthylmethyl) piperazine. Results: Fourteen strains were found to be multi-resistant. Each strain was found to have the bla(OXA-58) gene with the ISAba3-like element upstream, responsible for over-expression of the latter and subsequent carbapenem resistance. Similarly, ISAba1, upstream of the bla(ADC) ...
Objective To investigate the in vitro and in vivo antibacterial activities of tigecycline and other 13 common antimicrobial agents, alone or in combination, against multi-drug resistant Acinetobacter baumannii.MethodsAn in vitro susceptibility test of 101 Acinetobacter baumannii was used to detect minimal inhibitory concentrations (MICs). A mouse lung infection model of multi-drug resistant Acinetobacter baumannii,established by the ultrasonic atomization method, was used to define in vivo antimicrobial activities.Results Multi-drug resistant Acinetobacter baumannii showed high sensitivity to tigecycline (98% inhibition), polymyxin B (78.2% inhibition), and minocycline (74.2% inhibition). However, the use of these antimicrobial agents in combination with other antimicrobial agents produced synergistic or additive effects. In vivo data showed that white blood cell (WBC) counts in drug combination groups C (minocycline + amikacin) and D (minocycline + rifampicin) were significantly higher than in groups A
Acinetobacter baumannii has emerged as an important nosocomial pathogen due to its increasing resistance to most, if not all, antibiotics in clinical use. We recently reported the occurrence of extensive-drug resistant (XDR) A. baumannii isolates in a Malaysian tertiary hospital. The genome of one of these XDR isolates, A. baumannii AC12, was completely sequenced and comparative genome analyses performed to elucidate the genetic basis of its antimicrobial resistance. The A. baumannii AC12 genome consists of a 3.8 Mbp circular chromosome and an 8,731 bp cryptic plasmid, pAC12. It belongs to the ST195 lineage and is most closely related to A. baumannii BJAB0715 as well as other strains of the International Clone III (IC-III) group. Two antibiotic resistance islands (RIs), designated AC12-RI1 and AC12-RI2, were found in the AC12 chromosome along with a 7 kb Tn1548::armA island, conferring resistance to aminoglycosides and macrolides. The 22.8 kb AC12-RI1 interrupts the comM gene and harbours the ...
Patients with AB bacteremia receiving antimicrobial therapy are eligible for this multicenter study. Antimicrobial agents are decided at the discretion of the attending clinical team. Clinical data to be collected include patient demographics (age, gender, underlying diseases, Pitt Bacteremia Score [20], duration of ICU stay and hospitalization before the day of first positive blood culture, central venous catheterization), antimicrobial agents on the day of bacteremia, regimens and durations of combination therapy after enrollment, and outcomes (sequential quantification change of blood A. baumannii polymerase chain reaction [PCR], survival at day 30 after enrollment, and adverse drug reactions of antimicrobial agents). Blood sample will be collected on the day of enrollment (Day 0), Day 1, 2, 3 and 7 for PCR quantification of A. baumannii and for genospecies identification. Primary end points are the interval from study enrollment to negative blood A. baumannii PCR and blood sterilization. ...
Background & objective: Multidrug-resistant Acinetobacter baumannii (MDR-AB) is an important nosocomial pathogen which is associated with significant morbidity and mortality, particularly in high-risk populations. Aminoglycoside-modifying enzymes (AMEs) and 16S ribosomal RNA (16S rRNA) methylation are two important mechanisms of resistance to aminoglycosides. The aim of this study was to determine the prevalence of 16S rRNA methylase (armA, rmtA, rmtB, rmtC, and rmtD), and the AME genes [aac(6′)-Ib, aac(3)-I, ant(3′′)-I, aph(3′)-I and aac(6)-Id], among clinical isolates of A. baumannii in Tehran, Iran. Methods: Between November 2015 to July 2016, a total of 110 clinical strains of A. baumannii were isolated from patients in two teaching hospitals in Tehran, Iran. Antimicrobial susceptibility testing was performed according to Clinical and Laboratory Standards Institute guidelines. The presence of genes encoding the AMEs and16S rRNA methylases responsible for resis-tance was investigated by
This unit describes basic protocols for infecting mice through intranasal and intraperitoneal routes with Acinetobacter baumannii to induce associated pneumonia and sepsis, the two most common manifestations of clinical infections with this pathogen
Acinetobacter baumannii is an emerging nosocomial pathogen, responsible for infection outbreaks worldwide. The pathogenicity of this bacterium is mainly due to its multidrug-resistance and ability to form biofilm on abiotic surfaces, which facilitate long-term persistence in the hospital setting. Given the crucial role of iron in A. baumannii nutrition and pathogenicity, iron metabolism has been considered as a possible target for chelation-based antibacterial chemotherapy. In this study, we investigated the effect of iron restriction on A. baumannii growth and biofilm formation using different iron chelators and culture conditions. We report substantial inter-strain variability and growth medium-dependence for biofilm formation by A. baumannii isolates from veterinary and clinical sources. Neither planktonic nor biofilm growth of A. baumannii was affected by exogenous chelators. Biofilm formation was either stimulated by iron or not responsive to iron in the majority of isolates tested, ...
Acinetobacter baumannii outer membrane protein A targets the nucleus and induces cytotoxicity.: Acinetobacter baumannii is an emerging opportunistic pathogen re
To investigate the correlation between carbapenem consumption and rates of antimicrobial resistance in Acinetobacter baumannii, carbapenem consumption was expressed as defined daily dose based on the World Health Organization (WHO) anatomical therapeutic chemical classification index. Clinical isolates from 2001-2009 were collected and analyzed using WHONET 5.4 software. Results show that the consumption of imipenem/cilastatin, meropenem, and total carbapenem is significantly correlated with imipenem resistance in A baumannii (r = 0.818, P = .007; r = 0.817, P = .007; r = 0.827, P = .006). Furthermore, total carbapenem consumption is significantly correlated with meropenem resistance in A baumannii (r = 0.900, P = .037). In addition, consumption of imipenem/cilastatin, meropenem, and total carbapenem is associated with A baumannii resistance to piperacillin-tazobactam, ceftazidime, cefepime, amikacin, and levofloxacin. These drugs are mainly β-lactams, aminoglycosides, and fluoroquinolones. The ...
Multi-drug resistant Acinetobacter baumannii bacteria inside biofilm, computer illustration. A. baumannii is a Gram-negative, oxidase negative, aerobic, coccobacillus. It has always been naturally resistant to multiple antibiotics. It can be especially resistant to penicillin and chloramphenicol. It causes various nosocomial infections, including, skin and wound infections, pneumonia, meningitis, septicaemia, urinary tract infection and endocarditis. It is commonly found in soil, water, sewage, and normal skin and gastrointestinal tract flora. It is the most frequently encountered species in the clinical laboratory. Species found in soil can colonize root nodule systems and oxidize the hydrogen produced by nitrogen fixing bacteria. The illustration shows morphology of Acinetobacter such as short rods and sometimes long filamentous cells. - Stock Image F018/1264
A. baumannii the most clinically relevant of the bacteria tested, have a particular propensity for nosocomial transmission, due in part to their sustained survival on environmental surfaces as well as their multidrug resistance. The prevalence of infection with A. baumannii has increased significantly during the last decade. Moreover, A. baumannii have developed one of the most impressive patterns of antibiotic resistance ever observed. Therefore, rapid detection of A. baumannii in clinical samples could improve the targeted implementation of infection control measures and potentially aid the selection of empirical therapies for infected patients [3,5].. To culture A. baumannii isolated from clinical samples and to screen for carriers among intensive care unit patients, use of a selective enrichment medium is recommended. In recent years, a few previous reports about CHROMagar Acinetobacter® have been published with regard to multidrug resistant A. baumannii (MDRAB). Gordon et al. [6] reported ...
Rates of A. baumannii bacteraemia significantly increased between 2005 and 2009, from 0.1 to 3.2 cases/100,000 inhabitants per year. The observed increase was due to carbapenem-resistant isolates, while the number of carbapenem-susceptible isolates remained substantially stable over the study period. Importantly, the occurrence of carbapenem-resistant isolates showed a steep five-fold increase between 2008 and 2009. These isolates belonged to an epidemic strain detected in several departments of 4 hospital trusts in the Region. Similar trends were observed for urine and respiratory isolates. The total number of isolates in blood, urine and respiratory specimens, including both colonizing and infecting strains, increased from 51 in 2005 to 826 in 2009, with rates rising from 1.5 to 19.0 isolates/100,000 inhabitants per year. ...
Acinetobacter baumannii may exhibit phenotypic heterogeneous growth under exposure to antibiotics. We investigated the in vitro characteristics of A. baumannii isolates grown heterogeneously in the presence of meropenem and their virulence evaluated in experimental infections treated with meropenem. Five clinical A. baumannii isolates and the respective heterogeneously grown subpopulations were tested by agar dilution minimum inhibitory concentration (MIC) testing, pulsed field gel electrophoresis (PFGE), population analysis using meropenem and growth curves. The virulence of isolates and the therapeutic efficacy of three meropenem dosing schemes was evaluated in a neutropenic murine thigh infection model. The clinical isolates were meropenem-susceptible (MICs 1 to 4 mg/liter) and exhibited three distinct PFGE patterns. In all clinical isolates, population analysis yielded heterogeneously grown colonies. After seven subcultures in antibiotic-free media, resistant MIC levels were retained in two isolates
INTRODUCTION: The incidence of multidrug resistant microorganisms worldwide is increasing. The aim of the study was to present institutional experience with the multidrug resistant microorganism colonization patterns observed in children with congenital heart diseases hospitalized in a hybrid pediatric cardiac surgery center. MATERIAL AND METHODS: Microbiological samples were routinely collected in all children admitted to our department. All microbiological samples were analyzed with regard to multidrug resistant microorganisms: methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci (VRE), Gram-negative rods producing extended-spectrum beta-lactamases (ESBL), multidrug resistant Gram-negative rods (MDR-GNRs), carbapenemase-producing Klebsiella pneumoniae (KPC), carbapenem-resistant Acinetobacter baumannii (CRAB) and Pseudomonas aeruginosa (CRPA ...
More people come forward, bit by bit, telling stories of how the hospital played down their infection. The one person who could have done something about it, "Rep. Dennis Moore" has walked away from the issue deciding it wasnt worth getting into even after what he had seen on a visit to Walter Reed. Acinetobacter baumannii infections among patients at military medical facilities treating injured U.S. service members in 2002-2004 ...
Background Acinetobacter baumannii has become one of the most serious causative agents of nosocomial infections due to its significant ability to survive on hospital surfaces. It is mainly an emerging opportunistic pathogen infecting patients in intensive care units. This study was aimed to identify the clinical isolates of A. baumannii and to investigate their heterogeneity using polymerase chain reaction (PCR)-based typing methods. Methods A total of 197 nonduplicate isolates recovered from a wide range of clinical samples were subjected to conventional cultural and biochemical tests. For those isolates that were preliminary identified as A. baumannii, rpoB-based PCR with subsequent restriction fragment length polymorphism (RFLP) using two restriction enzymes (TagI and HaeIII) was performed to investigate the genetic diversity of the strains and their presumptive relationships with different clinical presentation of the disease caused by this pathogen. Results In total, 50 isolates (25.4%) ...
Minimal inhibition concentration (MIC) is the lowest concentration of an antimicrobial agent that can inhibit the visible growth of a microorganism after overnight incubation. MIC determination is used as not only a diagnostic tool in treating bacterial infections for clinicians but also a research method in evaluating the efficacy of an antimicrobial. Multidrug resistance Acinetobacter baumannii (A. baumannii) has emerged in recent years. Accurate determination of resistance by MIC assay is important in coping with this superbug. Here we described a protocol for determining MIC for A. baumannii in hope of assisting researchers and physicians in confirming resistance of clinical isolates correctly.
The University of Maryland School of Medicine received a one-year bridge grant in the amount of $334,523 from the National Institute of Allergy and Infectious Diseases to continue development of a live bacterial vaccine against Acinetobacter baumannii bacteria, which can lead to lethal pneumonia as well as serious soft tissue infections. The principal investigator for the research is James Galen, PhD, Professor of Medicine and faculty in the Center for Vaccine Development.. Referred to as "Iraqibacter" in media reports, these bacteria have widely impacted U.S. military personnel deployed in the Middle East and Afghanistan. They pose a significant threat to public health because of a steady rise in the frequency of multidrug-resistant pathogens. "We are attempting to immunize humans against several key outer membrane proteins present on the surface of A. baumannii," said Dr. Galen, whose research for more than 15 years has focused on the construction of live-attenuated bacterial vaccines. "These ...
Acinetobacter baumannii bacteria, coloured scanning electron micrograph (SEM). This bacterium has developed resistance to a number of antibiotics and is increasingly seen in opportunistic infections in hospitals. It typically infects the lungs, leading to a form of pneumonia. It can develop resistance to antibiotics even as they are being used to treat an infection. Because of this, and because it is generally found in weakened patients, the mortality rate for infections with A. baumannii is high. In healthy individuals, however, it is a normal part of the skin flora. Magnification: x11,000 when printed 10 centimetres wide. - Stock Image B220/1513
In view of the large outbreaks of infection by Acinetobacter baumannii and Pseudomonas aeruginosa, researchers sought to determine risk factors for the occurrence and appropriate infection control measures. They observed that Acinetobacter outbreaks were mainly reported from intensive care units, after use of
Ubiquinone (UQ), also called coenzyme Q, and plastoquinone (PQ) are electron carriers in oxidative phosphorylation and photosynthesis, respectively. The quinoid nucleus of ubiquinone is derived from the shikimate pathway; 4-hydroxybenzoate is directly formed from chorismate in bacteria, while it can be formed from either chorismate or tyrosine in yeast. The following biosynthesis of terpenoid moiety involves reactions of prenylation, decarboxylation, and three hydroxylations alternating with three methylations. The order of these reactions are somewhat different between bacteria and yeast. Phylloquinone (vitamin K1), menaquinone (vitamin K2), and tocopherol (vitamin E) are fat-soluble vitamins. Phylloquinone is a compound present in all photosynthetic plants serving as a cofactor for photosystem I-mediated electron transport. Menaquinone is an obligatory component of the electron-transfer pathway in bacteria ...
Acinetobacter is an important nosocomial Gram-negative pathogen that is difficult to treat due to its increasing resistance towards almost all antimicrobials. Cluster of antibiotic resistance determinants in A. baumannii are often found in structures known as resistance islands (RIs). These RIs are usually transposable elements which can be transferred from one organism to another and thus play an essential role in the development of antimicrobial resistance. The objective of this study was to identify any RIs that may contribute to the extensive drug-resistance (XDR) phenotype in A. baumannii strain AC12 which was isolated from a tertiary hospital in Terengganu, Malaysia. ...
Scientists have recently identified a resistance protein that allows acinetobacter baumannii bacteria to survive chlorhexidine, an antiseptic commonly used in wipes, cleansers and mouthwashes in hospitals. The resistance protein has been called Acinetobacter Chlorhexidine Efflux, abbreviated to Ace.
Antibiotic-Resistant Acinetobacter baumannii Increasing Success Remains a Challenge as a Nosocomial Pathogen. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Open peer review is a system where authors know who the reviewers are, and the reviewers know who the authors are. If the manuscript is accepted, the named reviewer reports are published alongside the article. Pre-publication versions of the article and author comments to reviewers are available by contacting [email protected] All previous versions of the manuscript and all author responses to the reviewers are also available.. You can find further information about the peer review system here.. ...
Betts, J. W., Kelly, S. M. and Haswell, S. J. 2011, Antibacterial effects of theaflavin and synergy with epicatechin against clinical isolates of Acinetobacter baumannii and Stenotrophomonas maltophilia, International journal of antimicrobial agents, vol. 38, no. 5, pp. 421-425, doi: 10.1016/j.ijantimicag.2011.07.006. ...
Richmond, G. E., Chua, Kim Lee, Piddock, L. J. V. (2013-03-09). Efflux in Acinetobacter baumannii can be determined by measuring accumulation of H33342 (bis-benzamide). Journal of Antimicrobial Chemotherapy 68 (7) : 1594-1600. [email protected] Repository. https://doi.org/10.1093/jac/ ...
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Acinetobacter baumannii.
Acinetobacter (/ˌæsɪˈniːtoʊbæktər/) is a genus of Gram-negative bacteria belonging to the wider class of Gammaproteobacteria. Acinetobacter species are oxidase-negative, exhibit twitching motility, and occur in pairs under magnification. They are important soil organisms, where they contribute to the mineralization of, for example, aromatic compounds. Acinetobacter species are a key source of infection in debilitated patients in the hospital, in particular the species Acinetobacter baumannii. Species of the genus Acinetobacter are strictly aerobic, nonfermentative, Gram-negative bacilli. They show mostly a coccobacillary morphology on nonselective agar. Rods predominate in fluid media, especially during early growth. The morphology of Acinetobacter species can be quite variable in Gram-stained human clinical specimens, and cannot be used to differentiate Acinetobacter from other common causes of infection. Most strains of Acinetobacter, except some of the A. lwoffii strain, grow well on ...
Multiple drug resistance Acinetobacter baumannii (MDRAB) ranks top among the nosocomial pathogen due to their environmental elasticity, ability to colonize various body sites of hospitalized patients, long-time persistence, association with multiple drug resistance and their successful outbreak potential [1-5]. It causes a wide spectrum of nosocomial infections which includes infections of bloodstream, urinary and respiratory tract, and ventilator associated pneumonia commonly among Intensive Care Unit patients (ICU) [6,7].. The main sources of transmission implicated with A. baumannii infections are person to person contact or through contaminated surface [8] and previous room occupancy by patients with A. baumannii infection or colonization. Epidemiological studies have clearly shown that hospital environment and colonized patients as the major reservoirs of A. baumannii infections [9]. Management of A. baumannii infections is the greatest challenge for patients, clinicians and infection ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
The TWiM team brings you a bacterium from a Colorado field site that grows on uranium, and copper resistance in the emerging pathogen Acinetobacter baumannii.
The importance of Acinetobacter baumannii infections in war-related injuries is now well established. A. baumannii was the most common gram-negative bacillus recovered from traumatic injuries to the lower extremities during the Vietnam War. More recently a new series of infections was reported in U.S. service personnel injured in the Iraq/Kuwait/Afghanistan regions. Likewise, A. baumannii has become an emerging pathogen of increasing importance in Veterans Administration and civilian healthcare facilities, with the incidence of A. baumannii infection increasing worldwide. This emergence of A. baumannii is due in large part to its ability to survive under a wide range of environmental conditions including those within healthcare facilities. Further, infections on foreign bodies such as intravascular devices and orthopedic hardware are virtually impossible to cure with antimicrobials alone. In addition multi- and pandrug resistant isolates are increasing. Thus, there is an increasing concern that ...
BACKGROUND: Acinetobacter baumannii is a nosocomial pathogen which is establishing as a major cause of morbidity and mortality within the healthcare community. The success of this pathogen is largely due to its ability to rapidly gain resistance to antimicrobial therapies and its capability to persist in an abiotic environment through the production of a biofilm. Our tertiary-care hospital has showed high incidence of carbapenem-resistant Acinetobacter baumannii (CRAB) isolates. METHODS: In this study we explore both genotypic and phenotypic properties of 26 CRAB isolates: 16 isolates were collected from January 2010 to March 2011, and 10 were collected between February and May 2015 ...
The research, published in Nature Microbiology, has been described by media as a major breakthrough that "could change the face of modern medicine." Lam has successfully used the polymers to kill six different superbugs in her lab and another superbug in mice. The technique has effectively fought off infections from drug-resistant Acinetobacter baumannii, a bacteria thats involved with pneumonia, meningitis, and urinary tract infections.. But its still too early to celebrate. Lam hasnt tested the polymers on superbugs in humans yet, and she could need another five years to fully develop the technique, her supervisor says. "With research, you need to have a lot of patience," Lam told the Telegraph (which, ahem, published its article about her discovery on the "Lifestyle-Women" section of its site).. Right now there seem to be few alternatives. As my colleague Tom Philpott has reported, scientists continue to discover more cases of bacteria that have evolved to resist the antibiotics we have. ...
Acinetobacter baumannii (strain ACICU) is an important opportunistic pathogen worldwide responsible for large outbreaks of nosocomial infection which account for 2-10% of all Gram-negative infections. A. baumannii infections include nosocomial pneumonia, secondary meningitis, skin, soft tissue and urinary tract infections and bacteremia, and result in high (up to 50%) morbidity and mortality. A. baumannii has simple growth requirements, exploiting a variety of nutritional sources, and is adaptable to a range of temperature, pH, salinity and humidity. Infections by this organism are becoming increasingly problematic due to the high number of resistance genes found in clinical isolates. Multidrug resistant clones of A. baumannii are emerging and spreading throughout many geographic areas. The emergence of two pan-European epidemic clones, referred to as European clones I and II, has been reported in north-western Europe since the1980s, and has been then documented in many European regions. These ...
Journal of Shahid Sadoughi University of Medical Sciences ماهنامه علمی پژوهشی دانشگاه علوم پزشکی و خدمات بهداشتی درمانی شهید صدوقی یزد
The whole-genome sequence of the multiply antibiotic resistant Acinetobacter baumannii isolate RCH51 belonging to sequence type ST103 (Institut Pasteur scheme) revealed that the set of genes at the capsule locus, KL24, includes four genes predicted to direct the synthesis of 3-acetamido-3,6-dideoxy-d-galactose (d-Fuc3NAc), and this sugar was found in the capsular polysaccharide (CPS). One of these genes, fdtE, encodes a novel bifunctional protein with an N-terminal FdtA 3,4-ketoisomerase domain and a C-terminal acetyltransferase domain. KL24 lacks a gene encoding a Wzy polymerase to link the oligosaccharide K units to form the CPS found associated with isolate RCH51, and a wzy gene was found in a small genomic island (GI) near the cpn60 gene. This GI is in precisely the same location as another GI carrying wzy and atr genes recently found in several A. baumannii isolates, but it does not otherwise resemble it. The CPS isolated from RCH51, studied by sugar analysis and 1D and 2D 1H and 13C NMR
Acintebacter baumannii is one of the most common emerging healthcare-associated human pathogens that currently cause numerous hospital outbreaks, and increasingly reported as a cause of human infection, particularly ventilator-associated pneumonia, bloodstream infections, urinary tract infections, and wound infections. This organism is increasingly showing multi-drug resistance (MDR) to various classes of antimicrobials and disinfectants. Using various molecular biology techniques, such as recombinant DNA technology, DNA sequencing and computer-aided DNA sequence analysis, site-directed mutagenesis, protein analysis, and protein structural studies we are trying to elucidate:. ...
Ive been told that I have an Acinetobacter Baumanni infection at a surgery site. Ive also been told not to worry about it and that the Augmentin I finished 10 days ago would clear it up ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Acinetobacter baumannii is a pathogenic species commonly isolated from the hospital environment and hospitalized patients. It is an aquatic organism, and is often cultured from liquid medical samples such as respiratory secretions, wounds, and urine. Acinetobacter also colonizes irrigating solutions and intravenous solutions. Although it has low virulence, it is capable of causing infection. Most isolates recovered from patients represent colonization rather than infection. When infections do occur, they usually occur in the blood, or in organs with a high fluid content, such as the lungs or urinary tract. Acinetobacter baumannii SDF is responsible for community-acquired infections, and is highly sensitive to antibiotics. This strain was isolated from the interior of body lice collected on homeless people living in France. Given that the louse interior is usually sterile, the presence of this strain can only be due to cryptic bacteremic episodes. Infections by this organism are becoming ...
Widespread dissemination of multidrug-resistant Acinetobacter baumannii producing OXA-23 carbapenemase and ArmA 16S ribosomal RNA methylase in a Bulgarian university hospital ...
The citrate cycle (TCA cycle, Krebs cycle) is an important aerobic pathway for the final steps of the oxidation of carbohydrates and fatty acids. The cycle starts with acetyl-CoA, the activated form of acetate, derived from glycolysis and pyruvate oxidation for carbohydrates and from beta oxidation of fatty acids. The two-carbon acetyl group in acetyl-CoA is transferred to the four-carbon compound of oxaloacetate to form the six-carbon compound of citrate. In a series of reactions two carbons in citrate are oxidized to CO2 and the reaction pathway supplies NADH for use in the oxidative phosphorylation and other metabolic processes. The pathway also supplies important precursor metabolites including 2-oxoglutarate. At the end of the cycle the remaining four-carbon part is transformed back to oxaloacetate. According to the genome sequence data, many organisms seem to lack genes for the full cycle [MD:M00009], but contain genes for specific segments [MD:M00010 M00011 ...
Altun, Hatice Uludag and Bulut, Cemal and Sahin, Hunkar and Kinikli, Sami and Yagci, Server and Kudret Adiloglu, Ali and Pekcan Demiroz, Ali (2014) Antimicrobial Susceptibilities of Clinical Acinetobacter baumannii Isolates With Different Genotypes. Jundishapur Journal of Microbiology, 7 (12). p. 1. ISSN 2008-4161 ...
Representatives of hospitals and of groups with different carbapenem and aminoglycoside resistance patterns in our collection have been sequenced together with the GC1 and GC2 reference strains from the early 1980s. Trees based on single nucleotide polymorphisms reveal significant diversity in the Australian isolates from one clone and little in the other. Most of the genes conferring resistance to older antibiotics are in the chromosome clustered in one island in GC1 and two in GC2 isolates. However, each of these islands is continually evolving, losing and gaining resistance genes. Further variation arises from the acquisition of different plasmids carrying further resistance genes. Other major but unexpected differences arising within the clones affect the exopolysaccharides. The capsule is an important virulence determinant, and substitution of large chromosomal segments leads to many distinct loci for capsule biosynthesis in each clone.. ...
Open peer review is a system where authors know who the reviewers are, and the reviewers know who the authors are. If the manuscript is accepted, the named reviewer reports are published alongside the article. Pre-publication versions of the article and author comments to reviewers are available by contacting [email protected] All previous versions of the manuscript and all author responses to the reviewers are also available.. You can find further information about the peer review system here.. ...
Rastegar Lari, Abdolaziz and Ardebili, Abdollah and Talebi, Malihe and Azimi, Leila (2014) Effect of Efflux Pump Inhibitor Carbonyl Cyanide 3-Chlorophenylhydrazone on the Minimum Inhibitory Concentration of Ciprofloxacin in Acinetobacter baumannii Clinical Isolates. Jundishapur Journal of Microbiology, 7 (1). pp. 1-5. ISSN 2008-4161 ...
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
Using an experimental treatment that involves the use of bacteriophages, or viruses that target bacteria specifically, the team has successfully treated Tom Patterson, PhD, a psychiatric professor at the UCSD School of Medicine.. In 2015, Patterson contracted what appeared at the time to be pancreatitis, but it was soon discovered by a team of specialist doctors in Frankfurt, Germany, that Patterson had contracted an extremely rare, dangerous, virulent, and antimicrobial-resistant strain of the Acinetobacter baumannii pathogen.. After attempting to treat Pattersons condition with an increasingly dangerous cocktail of potent and hazardous antibiotics, Pattersons health began to fail rapidly, at which point he was airlifted to Thornton Hospital, at UCSD. With doctors unable to stop the spread of Acinetobacter baumannii superbug, Patterson was eventually placed into a medical coma in January 2016.. In March of the same year, a research team began treating Patterson with a unique phage therapy ...
A new study has found that the complex mixture of sugars, proteins and fats in mothers milk possesses certain properties that can protect babies against bacterial infections like Streptococci too. Researchers from Vanderbilt University looked at the antibacterial properties of breast milk and found that the sugars in it have antibacterial properties and also work to enhance the antibacterial properties of the proteins in breast milk.. ...
3ZPG: Structure of Diaminohydroxyphosphoribosylaminopyrimidine Deaminase/5-Amino-6-(5-Phosphoribosylamino)Uracil Reductase from Acinetobacter Baumannii.
Acinetobacter baumannii is a Gram-negative opportunistic human pathogen known to cause a range of infections in hospitals. Despite their recent emergence, strains of A. baumannii, resistant to essentially all routinely used antibiotics, have been isolated from clinical settings. Bioinformatic analysis identified more than 50 transporter systems with a putative role in drug efflux in the genome of A. baumannii ATCC17978, representing ~2% of all its protein coding ORFs. Based on an assumption that drug transport is often associated with over-expression of a relevant efflux system in the presence of the substrate, high-throughput quantitative reverse-transcriptase PCR (qRT-PCR) has been performed after shock treatments with sub-inhibitory concentrations of antibiotics and differential expression of genes was assessed. This strategy has led to the discovery of novel drug efflux systems and defined physiological functions for previously characterised and novel pumps in drug resistance ...
Acinetobacter baumannii is a gram-negative, non-fermenting aerobic bacterium which is often associated with hospital-acquired infections and known for its ability to develop resistance to antibiotics, form biofilms, and survive for long periods in hospital environments. In this study, we present two novel viruses, vB_AbaP_AS11 and vB_AbaP_AS12, specifically infecting and lysing distinct multidrug-resistant clinical A. baumannii strains with K19 and K27 capsular polysaccharide structures, respectively. Both phages demonstrate rapid adsorption, short latent periods, and high burst sizes in one-step growth experiments. The AS11 and AS12 linear double-stranded DNA genomes of 41,642 base pairs (bp) and 41,402 bp share 86.3% nucleotide sequence identity with the most variable regions falling in host receptor-recognition genes. These genes encode tail spikes possessing depolymerizing activities towards corresponding capsular polysaccharides which are the primary bacterial receptors. We described AS11 and AS12
Since the 2006 discovery of the Acinetobacter baumannii strain AYE AbaR1 resistance island, similar elements have been reported in numerous members of this species. As AbaR1 is distantly related to Tn7, we have renamed it TnAbaR1. TnAbaR transposons are known to carry multiple antibiotic resistance- and efflux-associated genes, although none have been experimentally studied en bloc. We deleted the TnAbaR transposon in A. baumannii A424, which we have designated TnAbaR23, and characterized independent deletion mutants DCO163 and DCO174. The NotI pulsed-field gel electrophoresis (PFGE) profile of strain DCO174 was consistent with targeted deletion of TnAbaR23 alone, but strain DCO163 apparently harbored a second large genomic deletion. Nevertheless, subtractive amplification targeting 52 TnAbaR and/or resistance-associated loci yielded identical results for both mutants and highlighted genes lost relative to strain A424. PCR mapping and genome sequencing revealed the entire 48.3-kb sequence of ...
The cost to control outbreaks can be staggering, and some institutions have been forced to close units in order to interrupt the transmission of Acinetobacter. There is a need to prevent transmission in the healthcare setting and keep the organism, especially multidrug-resistant isolates, from becoming endemic in an institution. To achieve this goal it is also important to control contamination of the environment, water or food. Careful personal and hand-hygiene should be observed. It is important that laboratories should embark on active surveillance to detect cultures and patients who are colonized with multidrug-resistant Acinetobacter as well as a community-based surveillance to determine carriage rates. Other measures successful in the control of outbreaks include isolation precautions for infected or colonized patients, patients relatives and staff, environmental disinfection, antimicrobial control, and unit closure if needed.. Implementing control of WW clones is a priority as these ...
Acinetobacter is a genus of opportunistic pathogens in the proteobacteria group, species of which are distributed in widespread, diverse habitats. It has garnered media attention because of an outbreak among soldiers in Iraq who contracted the species Acinetobacter baumannii. While it was initially thought that the bacteria had come from the Iraqi soil, it turns out that the bacteria were actually contracted in the militarys evacuation chain. At least 280 people, mostly soldiers returning from the battlefield, were infected with the disease, and at least 5 (non-active-duty soldiers) died. These bacteria can often be found as the cause of pneumonia in hospitalized patients, especially those dependent on ventilators in Intensive Care Units. The bacteria are most often contracted through the exposure of open wounds to contaminated soil. This makes it a problem during warfare (it was the second-leading cause of infection among troops during the Vietnam conflict) because of the high number of ...
Acinetobacter baumannii is a pleomorphic aerobic gram-negative bacillus (similar in appearance to Haemophilus influenzae on Gram stain) commonly isolated from the hospital environment and hospitalized patients. A baumannii is a water organism and preferentially colonizes aquatic environments.
Corrected Genome Sequence of Acinetobacter Baumannii Strain AB0057, an Antibiotic-Resistant Isolate from Lineage 1 of Global Clone 1 ...
Corrected Genome Sequence of Acinetobacter Baumannii Strain AB0057, an Antibiotic-Resistant Isolate from Lineage 1 of Global Clone 1 ...
Staphylococcus aureus is a Gram-positive bacterial pathogen that is reported to cause more U.S. deaths annually than HIV. Acinetobacter baumannii is a Gram-negative pathogen that is emerging as a predominant cause of infections within military personnel injured during Operation Iraqi Freedom and has recently caused deadly outbreaks in the U.S., South America, and Europe. In addition to causing high incidences of morbidity and mortality, both organisms have also developed resistance to all currently available antibiotics, further amplifying public health concern and accentuating the need for new antibiotics for the treatment of S. aureus and A. baumannii infections ...
1) Magnet S, et al. (2001) Resistance-nodulation-cell division-type efflux pump involved in aminoglycoside resistance in Acinetobacter baumannii strain BM4454.. Antimicrob Agents Chemother 45(12):3375-80 PubMed: 11709311 ...
1) Magnet S, et al. (2001) Resistance-nodulation-cell division-type efflux pump involved in aminoglycoside resistance in Acinetobacter baumannii strain BM4454.. Antimicrob Agents Chemother 45(12):3375-80 PubMed: 11709311 ...
Purpose. The purpose of this study was to investigate New Delhi metallo-β-lactamase (NDM) production among Gram-negative bacilli. Methodology. Antibiogram-resistotyping and detection of New Delhi metallo-β-lactamase (NDM) in clinical isolates of Klebsiella pneumoniae, Acinetobacter baumannii and Pseudomonas aeruginosa and comparative evaluation of the diagnostic performance of three phenotypic methods for NDM detection, with PCR considered as the gold standard, were performed. Minimum inhibitory concentration (MIC) of antibiotics against NDM-positive strains using E-tests and clonal relationship analysis using enterobacterial repetitive intergenic consensus (ERIC)-PCR in these strains were determined. Results. The most effective antibiotics against strains of the species K. pneumoniae were Colistin, Chloramphenicol and Tigecycline; against P. aeruginosa were Fosfomycin and Polymyxins, and against A. baumannii were Polymyxins, Ampicillin/Sulbactam and Minocycline. Overall, 66, 31 and 40 different
During the one-year period, 159 patients were followed up.Mean age was 61.82±16.81. VAP developed in 96 (60%) patients with 37.2/1000 ventilation days.The mean APCAHE II score was 24.32±5.94, and there was no difference between VAP and non-VAP patients.Median mechanical ventilation days for non-VAP patients were 3 days (1-15 days).Mortality rate for non-VAP patients was 88.5%, and the median time for death was 4th day of hospitalization.Median time for VAP development was 5.5 days (2-25 days).Acinetobacter baumannii (42%) and Pseudomonas aeruginosa (20%) were the most common pathogens.All microorganisms were multi-resistant.Imipenem resistance of A.baumannii and P.aeruginosa was 92% and 71%, respectively.The most significant risk factors for VAP were stay in hospital before MICU (OR:3.11) and length of stay in MICU (1.47). Mortality rate for VAP-patients was 80% and there was no statistically difference between the mortality rates of VAP and non-VAP patients.Median total cost of non-VAP ...
Columbus, OH - November 2016. The bacteria Acinetobacter baumannii is a widely-reported cause of healthcare-associated infections, particularly in adult intensive care units in developing countries. But a retrospective study of isolated cases at Nationwide Childrens Hospital found that Acinetobacter pittii recently emerged as the more common cause of infection among patients there.. At a time when hospitals are trying to eliminate all possible harm, the discovery drives home the importance of being vigilant and of taking advantage of the latest genotyping techniques, the researchers say.. As we try to characterize hospital acquired infections, its important to do good speciation of the bacteria, says Benjamin Kopp, MD, a pulmonologist and principal investigator in the Center for Microbial Pathogenesis at The Research Institute at Nationwide Childrens, and senior author of the study. The Acinetobacter complex contains many different species that can act differently, so you cant really ...
Comparison of A. baumannii WT and ΔpglL membrane extracts by 2D-DIGE.Analysis of the membrane proteome of A. baumannii WT strain (A), ΔpglL strain (B), and me
The emergence and spread of antimicrobial resistance (AMR) mechanisms in bacterial pathogens, coupled with the dwindling number of effective antibiotics, has created a global health crisis. Being able to identify the genetic mechanisms of AMR and predict the resistance phenotypes of bacterial pathogens prior to culturing could inform clinical decision-making and improve reaction time. At PATRIC (http://patricbrc.org/), we have been collecting bacterial genomes with AMR metadata for several years. In order to advance phenotype prediction and the identification of genomic regions relating to AMR, we have updated the PATRIC FTP server to enable access to genomes that are binned by their AMR phenotypes, as well as metadata including minimum inhibitory concentrations. Using this infrastructure, we custom built AdaBoost (adaptive boosting) machine learning classifiers for identifying carbapenem resistance in Acinetobacter baumannii, methicillin resistance in Staphylococcus aureus, and beta-lactam and ...
Acinetobacter, Acinetobacter Baumannii, Bacteria, Health, Healthcare, Hospitals, Infection, Infection Control, Infections, Klebsiella, Klebsiella Pneumoniae, Organization, Pneumonia, Prevalence, Public Health, Urinary Tract, World Health, World Health Organization
A new report today from the World Health Organization (WHO) argues that the antibiotics now in clinical development are not sufficient to counter rising antimicrobial resistance (AMR), particularly in the pathogens that present the greatest threat to human health. They are a particular danger to patients who are already sick and have fragile immune systems.. The agency also singled out gram-negative pathogens Pseudomonas aeruginosa, Acinetobacter baumannii and Enterobacteriaceae, including Klebsiella pneumoniae and Escherichia coli, as "the most critical priority for antibiotic research and development" because "strains are emerging worldwide that can not be treated with any of the antibiotics now on the market".. "There is an urgent need for more investment in research and development for antibiotic-resistant infections including tuberculosis (TB), otherwise we will be forced back to a time when people feared common infections and risked their lives from minor surgery", World Health ...
PILONETTO, Marcelo et al. Hospital gowns as a vehicle for bacterial dissemination in an intensive care unit. Braz J Infect Dis [online]. 2004, vol.8, n.3, pp.206-210. ISSN 1413-8670. http://dx.doi.org/10.1590/S1413-86702004000300003.. The microbiota from the uniforms of 31 professionals from the general intensive care unit was analyzed. The samples were collected in duplicate at the beginning and at the end of the work period. Total viable counts of microorganisms were determined; there was a significant increase in the counts at the end of the period, when compared with those obtained at the beginning. No significant difference was observed between the first and second counts obtained from the cuffs. However, differences were observed for the samples from the abdominal region. Among the isolated pathogens 11/18 were Staphylococcus aureus, 2/18 were Acinetobacter baumannii, 2/18 were Klebsiela pneumoniae and 1/18 were Serratia rubidae. Some of these isolates were multi-resistant to antibiotics. ...
The primary use of antibiotics in pharyngitis is to treat infection due to group A Streptococcus (GAS). GAS is the most common bacterial agent causing acute pharyngitis, and accounts for approximately 15 to 30 of cases in children and 5 to 10 of adults (44,45). However, streptococcal pharyngitis is difficult to differentiate from other causes (such as viral etiology) on clinical grounds (45). Definitive diagnosis of streptococcal pharyngitis is based on the identification of GAS in the throat.... ...
Risk factors for colonisation or infection by Acinetobacter baumannii resistant to carbapenems in adult patients hospitalised in Intensive Care Units in Bogota, Colombia ...
4I6K: CRYSTAL STRUCTURE OF PROBABLE 2-PYRONE-4,6-DICARBOXYLIC ACID HYDROLASE (TARGET EFI-505029) FROM Acinetobacter baumannii
After Dark,Well, I dont really think that its debatable that having sex with someone of your same gender is considered "gay" sex. Whether having gay sex makes you gay is really at the heart of the debate here, but before I get to that I would like to point out a rather glaring flaw in your query, that being your supposition that one would be attracted to ones clone. Personally, I could think of few things more repulsive than having sex with myself. This is not because I am disgusting or because I am amazingly shitty in the sack or anything like that. It isnt even because I have self-esteem problems or that I am not in the least bit attracted to females (actually, I rather dislike most girls). It probably has something to do with the fact that by having sex with my clone I would have an innate sense of how my clone would react to certain stimuli, and, in short, Id find it entirely too easy (and more than a little creepy) to know each and every reaction that my clone would have to certain ...
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Volume 48, no. 1, p. 224-228, 2004. Page 224, column 2, line 16: "risk of IRAB infection" should read "risk of IRAB occurrence.". Page 225, column 2, line 49: "IRAB" should read "imipenem-resistant Pseudomonas aeruginosa.". Page 225, column 2, line 51: "ISAB" should read "imipenem-susceptible P. aeruginosa.". ...
OmpA Binding Mediates the Effect of Antimicrobial Peptide LL-37 on Acinetobacter baumannii. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Acinetobacter junii is a species of bacteria. Its type strain is ATCC 17908. It can be pathogenic. This bacterium has been linked to nosocomial infections including catheter-related blood stream infections and cellulitis. Vaneechoutte, M.; De Baere, T.; Nemec, A.; Musilek, M.; Van Der Reijden, T. J. K.; Dijkshoorn, L. (2008). "Reclassification of Acinetobacter grimontii Carr et al. 2003 as a later synonym of Acinetobacter junii Bouvet and Grimont 1986". International Journal of Systematic and Evolutionary Microbiology. 58 (4): 937-940. doi:10.1099/ijs.0.65129-0. PMID 18398198. Bouvet, P. J. M.; Grimont, P. A. D. (1986). "Taxonomy of the Genus Acinetobacter with the Recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov. and Emended Descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffii". International Journal of Systematic Bacteriology. 36 (2): 228-240. doi:10.1099/00207713-36-2-228. ...
Acinetobacter spp. are a diverse group of Gram-negative bacteria frequently implicated in nosocomial infections. Genotypic methods have been instrumental in studying Acinetobacter, but few offer high resolution, rapid turnaround time, technical ease and high inter-laboratory reproducibility, which has hampered understanding of disease incidence, transmission patterns and diversity within this genus. Here, we further evaluated multilocus PCR electrospray ionization/ mass spectrometry (PCR/ESI-MS), a method that is simple and robust, and provides both species characterization and strain-level resolution of Acinetobacter spp. on a single platform. We examined 125 Acinetobacter isolates from 21 hospitals, laboratories and medical centres spanning four counties in Arizona, USA, using PCR/ESI-MS. We compared PCR/ESI-MS with an in-house amplified fragment length polymorphism (AFLP) genotyping scheme. PCR/ESI-MS demonstrated that Acinetobacter spp. from Arizonan hospitals had similar species and strain
海词词典,最权威的学习词典,专业出版acinetobacter johnsonii是什么意思,acinetobacter johnsonii的用法,acinetobacter johnsonii翻译和读音等详细讲解。海词词典:学习变容易,记忆很深刻。
Introduction: The aim of this study was to investigate the presence of carbapenemase production and carbapenem resistance mechanisms in 47 carbapenem resistant Klebsiella pneumoniae isolates by phenotypic confirmatory tests and molecular assay.. Methodology: Carbapenem resistance genes KPC, OXA-48 and NDM were investigated with the BD MAX CRE assay kit in the BD MAX real time PCR instrument. Modified Hodge test, MBL gradient strip test, D70C Carbapenemase Detection Set, Temocillin gradient strip test methods were used as phenotypic confirmatory tests. Clonal relationship between study isolates was investigated with pulsed-field gel electrophoresis.. Results: Analysis with BD MAX CRE assay revealed OXA-48 positivity in 17 (36%) strains, NDM positivity in 6 (13%) strains and coexistence of OXA-48 + NDM positivity in 8 (17%) strains. In 16 (34%) strains, none of the KPC, OXA-48 and NDM genes were detected. While MHT was the most sensitive phenotypic confirmatory test, D70C disc set had not been ...
The efficient control of the spread of carbapenemase producer Enterobacteriaceae, a major public health threat world-wide, requires knowledge on the molecular epidemiology and genetic background of the emerging carbapenemase genes. Our aim was to investigate the genetic support of New-Delhi metallo-beta-lactamase and OXA-48-type carbapenemase genes in carbapenem resistant Enterobacteriaceae isolated in the United Arab Emirates. Since these isolates are extremely drug resistant, leaving few treatment options, the efficacy of alternative antimicrobials was also assessed. Seven NDM-producer Enterobacteriaceae, collected during 2009-2011, and subsequently five NDM and OXA-48-type carbapenemase co-producer Klebsiella pneumoniae isolated during 2011-2013 in three hospitals of the Emirates, were characterized. Strains clonality and the transmissibility of carbapenemase genes were assessed. The genetic support of carbapenemases was studied by characterization of plasmids and by sequencing the surrounding
In this study, we found the prevalence of multidrug resistant P. aeruginosa and A. baumannii at Mulago Hospital to be high but comparable to rates reported by Pitout et al. in Kenya [11], Lee et al. in Korea [13] and Gu et al. in China [14]. Additionally, the rates in the current study are lower than those reported by Uma Karthika et al. in India [15]. This could reflect challenges in embracing antibiotic stewardship programmes, or the differences in the amounts of antibiotics consumed in each setting where those studies were conducted [16]. Importantly, in Uganda like many other low-income countries, antibiotics are readily available over the counter in community pharmacies [9, 17] and this portends high rates of antimicrobial resistance in low-income countries.. Furthermore, carbapenem resistance prevalence in this study remains low in isolates from patients in accordance with recent findings at Mulago [8, 10, 18], and significantly lower than rates from Latin America [19]. Yet, carbapenem ...
CRE trends during 2006-2015 in the VHA recapitulate the epidemic of carbapenem-resistant K. pneumoniae in the United States and indicate that a "second epidemic" of carbapenem-resistant E. cloacae complex appears to be unfolding. In the United States, the predominant carbapenem-resistant K. pneumoniae genotype is sequence type (ST) 258, which is associated with Tn4401, a mobile genetic element containing blaKPC (7). In contrast, the genetic background of carbapenem-resistant E. cloacae complex is not well defined. Analysis of carbapenem-resistant E. cloacae from the US Midwest and New York, NY, demonstrated dissemination of E. cloacae complex ST171 harboring the blaKPC-3 gene (2,3,8). Further analysis demonstrated that ST171 was associated with a Tn4401 variant within a pBK30683-like plasmid; however, various other plasmids in Enterobacter spp. also harbor blaKPC-3 (4). Of note, in a northeastern US hospital, one third of carbapenem-resistant E. cloacae contained carbapenemases and the rest ...
Of 1284 Bacteroides strains collected in Europe in 2000 for antibiotic susceptibility surveillance, 65 isolates displayed imipenem minimum inhibitory concentrations (MICs) , or =1 mg/L and were chosen for a thorough analysis of their resistance mechanism. Twenty-five of the isolates were positive for the cfiA carbapenem resistance gene. The resistance rates were 0.8% and 1.3% for imipenem and meropenem, respectively. In six of the strains, insertion sequence (IS) elements (IS613, IS614B, IS1186 and IS1187) activated the cfiA gene. However, other strains displayed at least elevated carbapenem MICs or were carbapenem resistant and produced measurable carbapenemase activities but did not harbour IS elements in the region upstream of the cfiA gene. The major determinant of carbapenem resistance in Bacteroides fragilis is production of CfiA metallo-beta-lactamase via activation of the cfiA gene by IS elements (higher level resistance) or by activation of its putative own promoter.. ...
Acinetobacter Baumannii News, Research  Acinetobacter Baumannii News, Research
ECDC highlights need for increased efforts to prevent carbapenem-resistant A. baumannii outbreaks Acinetobacter baumannii is ...
more infohttps://www.news-medical.net/?tag=/Acinetobacter+Baumannii
Acinetobacter baumannii  Acinetobacter baumannii
Each antibiotic is presented in three columns. The first column lists the name of the antibiotic. The middle column represents susceptibility in percent to that antibiotic. The 3rd column represents the number of isolates tested for that specific antibiotic ...
more infohttps://www.washoecounty.us/health/programs-and-services/communicable-diseases-and-epidemiology/healthcare_professionals/antimicrobial-resistance/antibiogram/acinetobacter-baumannii.php
Acinetobacter baumannii ATCC ® 43498™  Acinetobacter baumannii ATCC ® 43498™
... Designation: S2 TypeStrain=False Application: Assay of cefoperazone Assay of sulbactam ... Acinetobacter baumannii (ATCC® 43498™) Strain Designations: S2 / Type Strain: no / Biosafety Level: 2 ...
more infohttps://www.atcc.org/en/Products/Cells_and_Microorganisms/Bacteria/Quality_Control_Strains/Micro_Quality_Control_Strains/43498.aspx
Acinetobacter baumannii  Acinetobacter baumannii
... industrial applications and more information for Acinetobacter baumannii. ... Acinetobacter baumannii is an opportunistic pathogen and is a problem in the hospital setting in US and Europe. Many strains ... Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Moraxellaceae; Acinetobacter. Industrial uses or economic ...
more infohttp://thelabrat.com/protocols/Bacterialspecies/Acinetobacterbaumannii.shtml
Acinetobacter baumannii ATCC ® BAA-1710D-5™  Acinetobacter baumannii ATCC ® BAA-1710D-5™
Genomic DNA from Acinetobacter baumannii strain AYE (ATCC ® BAA-1710™) TypeStrain=False Application: ... Acinetobacter baumannii (ATCC® BAA-1710D-5™) Strain Designations: Genomic DNA from Acinetobacter baumannii strain AYE (ATCC® ... Acinetobacter baumannii plasmid p2ABAYE, complete sequence Nucleotide (GenBank) : NC_010410 Acinetobacter baumannii AYE, ... Acinetobacter baumannii ATCC® BAA-1710D-5™ freeze-dried Total DNA: At least 5 µg in 1X TE buffer. OD260/OD280: 1.6 to 2.0 ...
more infohttps://www.atcc.org/Products/All/BAA-1710D-5.aspx?slp=1?p=1&rel=%7B0%7D
Acinetobacter baumannii SDF chromosome, complete genome - Nucleotide - NCBI  Acinetobacter baumannii SDF chromosome, complete genome - Nucleotide - NCBI
Due to the size of this record, annotated features are not shown by default. Use "Customize view" section to change the display ...
more infohttps://www.ncbi.nlm.nih.gov/nuccore/NC_010400
Carbapenem-resistant Acinetobacter baumannii CRAB | GreenMedInfo  Carbapenem-resistant Acinetobacter baumannii CRAB | GreenMedInfo
Carbapenem-resistant Acinetobacter baumannii (CRAB)Anti-Tumor,Tumors,Iodine,Lectin-Induced Cancer,Vegetables: All,Thyroid ... Carbapenem-resistant Acinetobacter baumannii (CRAB) is a Sub of the following Topics. *Acinetobacter baumannii infection ... Carbapenem-resistant Acinetobacter baumannii (CRAB) Related Articles. Could Turmeric Save Us From The CDCs Nightmare Bacteria ... 1 Abstracts with Carbapenem-resistant Acinetobacter baumannii (CRAB) Research. Filter by Study Type. In Vitro Study. ...
more infohttp://www.greenmedinfo.com/disease/carbapenem-resistant-acinetobacter-baumannii-crab
urea carboxylase [Acinetobacter baumannii 1417041] - Protein - NCBI  urea carboxylase [Acinetobacter baumannii 1417041] - Protein - NCBI
urea carboxylase [Acinetobacter baumannii 1417041] urea carboxylase [Acinetobacter baumannii 1417041]. gi,588244904,gb, ...
more infohttps://www.ncbi.nlm.nih.gov/protein/EXF16662.1
Acinetobacter baumannii infection | GreenMedInfo | Disease | Natural  Acinetobacter baumannii infection | GreenMedInfo | Disease | Natural
This topic contains 2 study abstracts on Acinetobacter baumannii infection indicating that the following substances may be ... 1 Abstracts with Acinetobacter baumannii infection & Raspberry root Research. [x] Remove Focus on Raspberry root. Filter by ... Diseases : Anthrax, Bacillus anthracis, Carbapenem-resistant Acinetobacter baumannii (CRAB), MRSA. Pharmacological Actions : ... 1 Problem Substances Researched for Acinetobacter baumannii infection Name. AC. CK. Focus. ...
more infohttp://www.greenmedinfo.com/disease/acinetobacter-baumannii-infection?ed=73036
acinetobacter baumannii epidemic in Maryland hospital, page 1  acinetobacter baumannii epidemic in Maryland hospital, page 1
A. baumannii is an opportunistic infection that commonly "enters" through a vent, open wound (usually dressed/covered in ... acinetobacter baumannii epidemic in Maryland hospital. page: 1 #liveFeed1, #liveFeed2, #liveFeed3 { margin: 40px 2px 50px 4px; ...
more infohttp://www.abovetopsecret.com/forum/thread329290/pg1
Acinetobacter baumannii Bouvet and Grimont ATCC ® 19606D-5™  Acinetobacter baumannii Bouvet and Grimont ATCC ® 19606D-5™
Genomic DNA from Acinetobacter baumannii strain 2208 TypeStrain=True Application: Food testing ... Acinetobacter baumannii Bouvet and Grimont (ATCC® 19606D-5™) Strain Designations: Genomic DNA from Acinetobacter baumannii ... Acinetobacter baumannii Bouvet and Grimont ATCC® 19606D-5™ dried At least 5 µg in 1X TE buffer ... Nucleotide (GenBank) : AF100557 Acinetobacter baumannii DNA gyrase A (gyrA) gene, partial cds. ...
more infohttps://atcc.org/en/Products/Cells_and_Microorganisms/By_Focus_Area/Food_Applications/Quality_Control/19606D-5.aspx
Acinetobacter baumannii Bouvet and Grimont ATCC ® 19606D-5™  Acinetobacter baumannii Bouvet and Grimont ATCC ® 19606D-5™
Genomic DNA from Acinetobacter baumannii strain 2208 TypeStrain=True Application: Food testing ... Acinetobacter baumannii Bouvet and Grimont (ATCC® 19606D-5™) Strain Designations: Genomic DNA from Acinetobacter baumannii ... Acinetobacter baumannii Bouvet and Grimont ATCC® 19606D-5™ dried At least 5 µg in 1X TE buffer ... Nucleotide (GenBank) : AF100557 Acinetobacter baumannii DNA gyrase A (gyrA) gene, partial cds. ...
more infohttps://atcc.org/en/Products/Cells_and_Microorganisms/Bacteria/Bacterial_Nucleic_Acids/19606D-5.aspx
Prevalence of hypermutators among clinical Acinetobacter baumannii isolates  Prevalence of hypermutators among clinical Acinetobacter baumannii isolates
Objectives: The objectives of this study were to study the presence of mutators in a set of Acinetobacter baumannii isolates ... Prevalence of hypermutators among clinical Acinetobacter baumannii isolates. Komp Lindgren, Patricia Uppsala University, ... Methods: The variation in mutation rate was evaluated for 237 clinical A. baumannii isolates by determining the frequency of ...
more infohttp://www.diva-portal.org/smash/record.jsf?pid=diva2:895326
KEGG BRITE: KEGG Orthology (KO) - Acinetobacter baumannii AYE  KEGG BRITE: KEGG Orthology (KO) - Acinetobacter baumannii AYE
KEGG Orthology (KO) - Acinetobacter baumannii AYE [ Brite menu , Organism menu , Download htext , Download json ] ...
more infohttp://www.genome.jp/kegg-bin/get_htext?aby00001+ABAYE2333
RCSB PDB 









- 3ZPG: Acinetobacter baumannii RibD, form 2 Methods Report Page  RCSB PDB - 3ZPG: Acinetobacter baumannii RibD, form 2 Methods Report Page
3ZPG: Structure of Diaminohydroxyphosphoribosylaminopyrimidine Deaminase/5-Amino-6-(5-Phosphoribosylamino)Uracil Reductase from Acinetobacter Baumannii.
more infohttp://www.rcsb.org/pdb/explore/materialsAndMethods.do?structureId=3ZPG
Cloning, Expression, and Purification of Nucleoside Diphosphate Kinase from Acinetobacter baumannii  Cloning, Expression, and Purification of Nucleoside Diphosphate Kinase from Acinetobacter baumannii
T. D. Gootz and A. Marra, "Acinetobacter baumannii: an emerging multidrug-resistant threat," Expert Review of Anti-Infective ... B. A. Eijkelkamp, K. A. Hassan, I. T. Paulsen, and M. H. Brown, "Investigation of the human pathogen Acinetobacter baumannii ... Cloning, Expression, and Purification of Nucleoside Diphosphate Kinase from Acinetobacter baumannii. Juhi Sikarwar, Sanket ... A. Howard, M. ODonoghue, A. Feeney, and R. D. Sleator, "Acinetobacter baumannii: an emerging opportunistic pathogen," ...
more infohttps://www.hindawi.com/journals/er/2013/597028/ref/
Inhaled colistin on Acinetobacter baumannii treatment | European Respiratory Society  Inhaled colistin on Acinetobacter baumannii treatment | European Respiratory Society
Inhaled colistin on Acinetobacter baumannii treatment. Gustavo Coimbra dos Reis, Ana Martins, Luís Bento ... Inhaled colistin on Acinetobacter baumannii treatment Message Subject (Your Name) has sent you a message from European ... A. baumannii was extensively resistant in all cases, being sensible only to polymyxins in 20 (87%). Mean inhaled colistin diary ... Conclusions: On critical patients, inhaled colistin was effective treating PAV/TAV due to extensively resistant A. baumannii. ...
more infohttp://erj.ersjournals.com/content/40/Suppl_56/P1999
thiG - Thiazole synthase - Acinetobacter baumannii (strain ACICU) - thiG gene & protein  thiG - Thiazole synthase - Acinetobacter baumannii (strain ACICU) - thiG gene & protein
Acinetobacter baumannii (strain AYE). Acinetobacter baumannii. Acinetobacter calcoaceticus. Acinetobacter baumannii (strain ... Acinetobacter baumannii (strain AB307-0294). Acinetobacter baumannii (strain AB0057). Acinetobacter baumannii (strain ATCC ... Acinetobacter guillouiae (Acinetobacter genomosp. 11). Acinetobacter sp. ETR1. Acinetobacter sp. Root1280. And more. 261. ... Acinetobacter pittii (Acinetobacter genomosp. 3). Acinetobacter genomosp. 33YU. Acinetobacter nosocomialis. And more. 261. ...
more infohttp://www.uniprot.org/uniprot/B2HUU9
KEGG PATHWAY: Pentose phosphate pathway - Acinetobacter baumannii MDR-ZJ06  KEGG PATHWAY: Pentose phosphate pathway - Acinetobacter baumannii MDR-ZJ06
Pentose phosphate pathway - Acinetobacter baumannii MDR-ZJ06 [ Pathway menu , Organism menu , Pathway entry , Download KGML , ...
more infohttp://www.genome.jp/kegg-bin/show_pathway?abz00030
hdc - Histidine decarboxylase - Acinetobacter baumannii (strain AYE) - hdc gene & protein  hdc - Histidine decarboxylase - Acinetobacter baumannii (strain AYE) - hdc gene & protein
sp,B0VBU8,DCHS_ACIBY Histidine decarboxylase OS=Acinetobacter baumannii (strain AYE) OX=509173 GN=hdc PE=3 SV=1 ... Acinetobacter baumannii (strain AYE). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names_and_taxonomy_section ... cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › ...
more infohttps://www.uniprot.org/uniprot/B0VBU8
  • Introduction: Ventilator associated pneumonia and tracheobronchitis (VAP/VAT) due to multiresistant A. baumannii are preeminent causes of mortality and morbidity at ICU's. (ersjournals.com)
  • Material and Methods: Observational, longitudinal, retrospective study, through file consult, of patients admitted at UUM from 01/2005 to 12/2011, with A. baumannii VAP/TAV, treated with inhaled colistin. (ersjournals.com)
  • This is the first report showing a relationship between CAM influx and aromatic compound metabolism in A. baumannii . (springer.com)
  • Interestingly, temperature plays a role in the ability of A. baumannii to sense and respond to light via the BlsA photoreceptor protein. (asm.org)
  • In contrast, "non-ACB " Acinetobacter species generally present lower pathogenicity and are often found in the environment. (springer.com)
  • 4-Hydroxybenzaldehyde (4-HBA), which cannot support the growth of Acinetobacter baumannii , exhibited synergism only with amphenicol antibiotics including chloramphenicol (CAM) and thiamphenicol. (springer.com)
  • In July 2010, a team in New Delhi reported a cluster of three cases of Acinetobacter baumannii bearing blaNDM-1 that were found in the intensive care unit of a hospital in Chennai, India, in April 2010. (wikipedia.org)
  • The University Hospital and the New Jersey Department of Health are working together to control acinetobacter and are employing all possible methods to control any issue that may arise. (inquisitr.com)