Cells lining the saclike dilatations known as acini of various glands or the lungs.
A malignant tumor arising from secreting cells of a racemose gland, particularly the salivary glands. Racemose (Latin racemosus, full of clusters) refers, as does acinar (Latin acinus, grape), to small saclike dilatations in various glands. Acinar cell carcinomas are usually well differentiated and account for about 13% of the cancers arising in the parotid gland. Lymph node metastasis occurs in about 16% of cases. Local recurrences and distant metastases many years after treatment are common. This tumor appears in all age groups and is most common in women. (Stedman, 25th ed; Holland et al., Cancer Medicine, 3d ed, p1240; from DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, p575)
A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.
The major component (about 80%) of the PANCREAS composed of acinar functional units of tubular and spherical cells. The acinar cells synthesize and secrete several digestive enzymes such as TRYPSINOGEN; LIPASE; AMYLASE; and RIBONUCLEASE. Secretion from the exocrine pancreas drains into the pancreatic ductal system and empties into the DUODENUM.
The largest of the three pairs of SALIVARY GLANDS. They lie on the sides of the FACE immediately below and in front of the EAR.
A group of amylolytic enzymes that cleave starch, glycogen, and related alpha-1,4-glucans. (Stedman, 25th ed) EC 3.2.1.-.
A specific decapeptide obtained from the skin of Hila caerulea, an Australian amphibian. Caerulein is similar in action and composition to CHOLECYSTOKININ. It stimulates gastric, biliary, and pancreatic secretion; and certain smooth muscle. It is used in paralytic ileus and as diagnostic aid in pancreatic malfunction.
The tear-forming and tear-conducting system which includes the lacrimal glands, eyelid margins, conjunctival sac, and the tear drainage system.
A peptide, of about 33 amino acids, secreted by the upper INTESTINAL MUCOSA and also found in the central nervous system. It causes gallbladder contraction, release of pancreatic exocrine (or digestive) enzymes, and affects other gastrointestinal functions. Cholecystokinin may be the mediator of satiety.
One of two salivary glands in the neck, located in the space bound by the two bellies of the digastric muscle and the angle of the mandible. It discharges through the submandibular duct. The secretory units are predominantly serous although a few mucous alveoli, some with serous demilunes, occur. (Stedman, 25th ed)
INFLAMMATION of the PANCREAS. Pancreatitis is classified as acute unless there are computed tomographic or endoscopic retrograde cholangiopancreatographic findings of CHRONIC PANCREATITIS (International Symposium on Acute Pancreatitis, Atlanta, 1992). The two most common forms of acute pancreatitis are ALCOHOLIC PANCREATITIS and gallstone pancreatitis.
A slowly hydrolyzed CHOLINERGIC AGONIST that acts at both MUSCARINIC RECEPTORS and NICOTINIC RECEPTORS.
Glands that secrete SALIVA in the MOUTH. There are three pairs of salivary glands (PAROTID GLAND; SUBLINGUAL GLAND; SUBMANDIBULAR GLAND).
The inactive proenzyme of trypsin secreted by the pancreas, activated in the duodenum via cleavage by enteropeptidase. (Stedman, 25th ed)
An octapeptide hormone present in the intestine and brain. When secreted from the gastric mucosa, it stimulates the release of bile from the gallbladder and digestive enzymes from the pancreas.
Ducts that collect PANCREATIC JUICE from the PANCREAS and supply it to the DUODENUM.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
A salivary gland on each side of the mouth below the TONGUE.
Aquaporin 5 is a water-specific channel protein that is expressed primarily in alveolar, tracheal, and upper bronchial EPITHELIUM. It plays an important role in maintaining water HOMEOSTASIS in the LUNGS and may also regulate release of SALIVA and TEARS in the SALIVARY GLANDS and the LACRIMAL GLAND.
Any of the ducts which transport saliva. Salivary ducts include the parotid duct, the major and minor sublingual ducts, and the submandibular duct.
Cell surface proteins that bind cholecystokinin (CCK) with high affinity and trigger intracellular changes influencing the behavior of cells. Cholecystokinin receptors are activated by GASTRIN as well as by CCK-4; CCK-8; and CCK-33. Activation of these receptors evokes secretion of AMYLASE by pancreatic acinar cells, acid and PEPSIN by stomach mucosal cells, and contraction of the PYLORUS and GALLBLADDER. The role of the widespread CCK receptors in the central nervous system is not well understood.
A bile salt formed in the liver from lithocholic acid conjugation with taurine, usually as the sodium salt. It solubilizes fats for absorption and is itself absorbed. It is a cholagogue and choleretic.
Vesicles derived from the GOLGI APPARATUS containing material to be released at the cell surface.
Antibiotic substance produced by various Streptomyces species. It is an inhibitor of enzymatic activities that involve glutamine and is used as an antineoplastic and immunosuppressive agent.
Condensed areas of cellular material that may be bounded by a membrane.
Tumors or cancer of the PANCREAS. Depending on the types of ISLET CELLS present in the tumors, various hormones can be secreted: GLUCAGON from PANCREATIC ALPHA CELLS; INSULIN from PANCREATIC BETA CELLS; and SOMATOSTATIN from the SOMATOSTATIN-SECRETING CELLS. Most are malignant except the insulin-producing tumors (INSULINOMA).
Drugs that bind to and activate cholinergic receptors.
A neurotransmitter found at neuromuscular junctions, autonomic ganglia, parasympathetic effector junctions, a subset of sympathetic effector junctions, and at many sites in the central nervous system.
A severe form of acute INFLAMMATION of the PANCREAS characterized by one or more areas of NECROSIS in the pancreas with varying degree of involvement of the surrounding tissues or organ systems. Massive pancreatic necrosis may lead to DIABETES MELLITUS, and malabsorption.
A peptide hormone of about 27 amino acids from the duodenal mucosa that activates pancreatic secretion and lowers the blood sugar level. (USAN and the USP Dictionary of Drug Names, 1994, p597)
Cellular release of material within membrane-limited vesicles by fusion of the vesicles with the CELL MEMBRANE.
Glands of external secretion that release its secretions to the body's cavities, organs, or surface, through a duct.
Intracellular messenger formed by the action of phospholipase C on phosphatidylinositol 4,5-bisphosphate, which is one of the phospholipids that make up the cell membrane. Inositol 1,4,5-trisphosphate is released into the cytoplasm where it releases calcium ions from internal stores within the cell's endoplasmic reticulum. These calcium ions stimulate the activity of B kinase or calmodulin.
Signal transduction mechanisms whereby calcium mobilization (from outside the cell or from intracellular storage pools) to the cytoplasm is triggered by external stimuli. Calcium signals are often seen to propagate as waves, oscillations, spikes, sparks, or puffs. The calcium acts as an intracellular messenger by activating calcium-responsive proteins.
The fluid containing digestive enzymes secreted by the pancreas in response to food in the duodenum.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
The discharge of saliva from the SALIVARY GLANDS that keeps the mouth tissues moist and aids in digestion.
Physiologically inactive substances that can be converted to active enzymes.
A tetradecapeptide originally obtained from the skins of toads Bombina bombina and B. variegata. It is also an endogenous neurotransmitter in many animals including mammals. Bombesin affects vascular and other smooth muscle, gastric secretion, and renal circulation and function.
A sesquiterpene lactone found in roots of THAPSIA. It inhibits CA(2+)-TRANSPORTING ATPASE mediated uptake of CALCIUM into SARCOPLASMIC RETICULUM.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
INFLAMMATION of the PANCREAS that is characterized by recurring or persistent ABDOMINAL PAIN with or without STEATORRHEA or DIABETES MELLITUS. It is characterized by the irregular destruction of the pancreatic parenchyma which may be focal, segmental, or diffuse.
Drugs that bind to and activate muscarinic cholinergic receptors (RECEPTORS, MUSCARINIC). Muscarinic agonists are most commonly used when it is desirable to increase smooth muscle tone, especially in the GI tract, urinary bladder and the eye. They may also be used to reduce heart rate.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
2-Amino-4-(ethylthio)butyric acid. An antimetabolite and methionine antagonist that interferes with amino acid incorporation into proteins and with cellular ATP utilization. It also produces liver neoplasms.
Organic compounds which contain tin in the molecule. Used widely in industry and agriculture.
A fluorescent calcium chelating agent which is used to study intracellular calcium in tissues.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The removal or interruption of some part of the parasympathetic nervous system for therapeutic or research purposes.
Intracellular receptors that bind to INOSITOL 1,4,5-TRISPHOSPHATE and play an important role in its intracellular signaling. Inositol 1,4,5-trisphosphate receptors are calcium channels that release CALCIUM in response to increased levels of inositol 1,4,5-trisphosphate in the CYTOPLASM.
An alkaloid, originally from Atropa belladonna, but found in other plants, mainly SOLANACEAE. Hyoscyamine is the 3(S)-endo isomer of atropine.
A group of compounds that are derivatives of beta-methylacetylcholine (methacholine).
Pathological processes of the PANCREAS.
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
A genetically related subfamily of RAB GTP-BINDING PROTEINS involved in calcium-dependent EXOCYTOSIS. This enzyme was formerly listed as EC 3.6.1.47.
Isopropyl analog of EPINEPHRINE; beta-sympathomimetic that acts on the heart, bronchi, skeletal muscle, alimentary tract, etc. It is used mainly as bronchodilator and heart stimulant.
Cell membrane glycoproteins that form channels to selectively pass chloride ions. Nonselective blockers include FENAMATES; ETHACRYNIC ACID; and TAMOXIFEN.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Acute or chronic INFLAMMATION of the PANCREAS due to excessive ALCOHOL DRINKING. Alcoholic pancreatitis usually presents as an acute episode but it is a chronic progressive disease in alcoholics.
Chronic inflammatory and autoimmune disease in which the salivary and lacrimal glands undergo progressive destruction by lymphocytes and plasma cells resulting in decreased production of saliva and tears. The primary form, often called sicca syndrome, involves both KERATOCONJUNCTIVITIS SICCA and XEROSTOMIA. The secondary form includes, in addition, the presence of a connective tissue disease, usually rheumatoid arthritis.
Inorganic compounds derived from hydrochloric acid that contain the Cl- ion.
A number of different cardioactive glycosides obtained from Strophanthus species. OUABAIN is from S. gratus and CYMARINE from S. kombe. They are used like the digitalis glycosides.
A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.
A subclass of alpha-amylase ISOENZYMES that are secreted into PANCREATIC JUICE.
Carcinoma that arises from the PANCREATIC DUCTS. It accounts for the majority of cancers derived from the PANCREAS.
Proteins and peptides found in SALIVA and the SALIVARY GLANDS. Some salivary proteins such as ALPHA-AMYLASES are enzymes, but their composition varies in different individuals.
The fluid secreted by the lacrimal glands. This fluid moistens the CONJUNCTIVA and CORNEA.
An eleven-amino acid neurotransmitter that appears in both the central and peripheral nervous systems. It is involved in transmission of PAIN, causes rapid contractions of the gastrointestinal smooth muscle, and modulates inflammatory and immune responses.
Inflammation of the lacrimal sac. (Dorland, 27th ed)
The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).
A subtype of cholecystokinin receptor found primarily in the PANCREAS; STOMACH; INTESTINE; and GALLBLADDER. It plays a role in regulating digestive functions such as gallbladder contraction, pancreatic enzyme secretion and absorption in the GASTROINTESTINAL TRACT.
Disease having a short and relatively severe course.
The clear, viscous fluid secreted by the SALIVARY GLANDS and mucous glands of the mouth. It contains MUCINS, water, organic salts, and ptylin.
The extracellular moiety of the POLYMERIC IMMUNOGLOBULIN RECEPTOR found alone or complexed with IGA or IGM, in a variety of external secretions (tears, bile, colostrum.) Secretory component is derived by proteolytic cleavage of the receptor during transcytosis. When immunoglobulins IgA and IgM are bound to the receptor, during their transcytosis secretory component becomes covalently attached to them generating SECRETORY IMMUNOGLOBULIN A or secretory IMMUNOGLOBULIN M.
Secretory cells of the ductless glands. They secrete HORMONES directly into the blood circulation (internal secretion) to be carried to the target cells. The secreted chemicals can be PEPTIDES; STEROIDS; NEUROPEPTIDES; or BIOGENIC AMINES.
Elements of limited time intervals, contributing to particular results or situations.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
A pyridine nucleotide that mobilizes CALCIUM. It is synthesized from nicotinamide adenine dinucleotide (NAD) by ADP RIBOSE CYCLASE.
Inorganic compounds that contain ruthenium as an integral part of the molecule.
Star-shaped, myofibroblast-like cells located in the periacinar, perivascular, and periductal regions of the EXOCRINE PANCREAS. They play a key role in the pathobiology of FIBROSIS; PANCREATITIS; and PANCREATIC CANCER.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
An enzyme of the hydrolase class that catalyzes the reaction of triacylglycerol and water to yield diacylglycerol and a fatty acid anion. It is produced by glands on the tongue and by the pancreas and initiates the digestion of dietary fats. (From Dorland, 27th ed) EC 3.1.1.3.
The proteinaceous component of the pancreatic stone in patients with PANCREATITIS.
A major class of calcium-activated potassium channels that were originally discovered in ERYTHROCYTES. They are found primarily in non-excitable CELLS and set up electrical gradients for PASSIVE ION TRANSPORT.
A chelating agent relatively more specific for calcium and less toxic than EDETIC ACID.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Phosphoric acid esters of inositol. They include mono- and polyphosphoric acid esters, with the exception of inositol hexaphosphate which is PHYTIC ACID.
The amount of a substance secreted by cells or by a specific organ or organism over a given period of time; usually applies to those substances which are formed by glandular tissues and are released by them into biological fluids, e.g., secretory rate of corticosteroids by the adrenal cortex, secretory rate of gastric acid by the gastric mucosa.
The rate dynamics in chemical or physical systems.
An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.
The physiological renewal, repair, or replacement of tissue.
N-(1-Oxobutyl)-cyclic 3',5'-(hydrogen phosphate)-2'-butanoate guanosine. A derivative of cyclic GMP. It has a higher resistance to extracellular and intracellular phosphodiesterase than cyclic GMP.
Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN.
A sulfamyl diuretic.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
An analog of benzilylcholine mustard. It is an alkylating nitrogen mustard analog that binds specifically and irreversibly to cholinergic muscarinic receptors and is used as an affinity label to isolate and study the receptors.
A ubiquitously expressed protein kinase that is involved in a variety of cellular SIGNAL PATHWAYS. Its activity is regulated by a variety of signaling protein tyrosine kinase.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
A class of compounds composed of repeating 5-carbon units of HEMITERPENES.
A widely distributed purinergic P2X receptor subtype that plays a role in pain sensation. P2X4 receptors found on MICROGLIA cells may also play a role in the mediation of allodynia-related NEUROPATHIC PAIN.
A highly basic, 28 amino acid neuropeptide released from intestinal mucosa. It has a wide range of biological actions affecting the cardiovascular, gastrointestinal, and respiratory systems and is neuroprotective. It binds special receptors (RECEPTORS, VASOACTIVE INTESTINAL PEPTIDE).
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.
A serine proteinase inhibitor used therapeutically in the treatment of pancreatitis, disseminated intravascular coagulation (DIC), and as a regional anticoagulant for hemodialysis. The drug inhibits the hydrolytic effects of thrombin, plasmin, and kallikrein, but not of chymotrypsin and aprotinin.
An oligopeptide isolated from the skin of Physalaemus fuscumaculatus, a South American frog. It is a typical kinin, resembling SUBSTANCE P in structure and action and has been proposed as a sialagogue, antihypertensive, and vasodilator.
A type of endoplasmic reticulum (ER) where polyribosomes are present on the cytoplasmic surfaces of the ER membranes. This form of ER is prominent in cells specialized for protein secretion and its principal function is to segregate proteins destined for export or intracellular utilization.
A derivative of benzodiazepine that acts on the cholecystokinin A (CCKA) receptor to antagonize CCK-8's (SINCALIDE) physiological and behavioral effects, such as pancreatic stimulation and inhibition of feeding.
A protease of broad specificity, obtained from dried pancreas. Molecular weight is approximately 25,000. The enzyme breaks down elastin, the specific protein of elastic fibers, and digests other proteins such as fibrin, hemoglobin, and albumin. EC 3.4.21.36.
Gastrointestinal agents that stimulate the flow of bile into the duodenum (cholagogues) or stimulate the production of bile by the liver (choleretic).
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
A cyclic nucleotide derivative that mimics the action of endogenous CYCLIC AMP and is capable of permeating the cell membrane. It has vasodilator properties and is used as a cardiac stimulant. (From Merck Index, 11th ed)
The pathological process occurring in cells that are dying from irreparable injuries. It is caused by the progressive, uncontrolled action of degradative ENZYMES, leading to MITOCHONDRIAL SWELLING, nuclear flocculation, and cell lysis. It is distinct it from APOPTOSIS, which is a normal, regulated cellular process.
HORMONES secreted by the gastrointestinal mucosa that affect the timing or the quality of secretion of digestive enzymes, and regulate the motor activity of the digestive system organs.
Voltage-dependent cell membrane glycoproteins selectively permeable to calcium ions. They are categorized as L-, T-, N-, P-, Q-, and R-types based on the activation and inactivation kinetics, ion specificity, and sensitivity to drugs and toxins. The L- and T-types are present throughout the cardiovascular and central nervous systems and the N-, P-, Q-, & R-types are located in neuronal tissue.
SNARE proteins where the central amino acid residue of the SNARE motif is an ARGININE. They are classified separately from the Q-SNARE PROTEINS where the central amino acid residue of the SNARE motif is a GLUTAMINE. This subfamily contains the vesicle associated membrane proteins (VAMPs) based on similarity to the prototype for the R-SNAREs, VAMP2 (synaptobrevin 2).
A naturally occurring phenomenon where terminally differentiated cells dedifferentiate to the point where they can switch CELL LINEAGES. The cells then differentiate into other cell types.
A primary malignant neoplasm of the pancreatic ISLET CELLS. Usually it involves the non-INSULIN-producing cell types, the PANCREATIC ALPHA CELLS and the pancreatic delta cells (SOMATOSTATIN-SECRETING CELLS) in GLUCAGONOMA and SOMATOSTATINOMA, respectively.
The abundant submucosal mucous glands in the DUODENUM. These glands secrete BICARBONATE IONS; GLYCOPROTEINS; and PEPSINOGEN II.
Chelating agent used for heavy metal poisoning and assay. It causes diabetes.
A benign neoplasm derived from glandular epithelium, in which cystic accumulations of retained secretions are formed. In some instances, considerable portions of the neoplasm, or even the entire mass, may be cystic. (Stedman, 25th ed)
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
Carboxypeptidases that are primarily found the DIGESTIVE SYSTEM that catalyze the release of C-terminal amino acids. Carboxypeptidases A have little or no activity for hydrolysis of C-terminal ASPARTIC ACID; GLUTAMIC ACID; ARGININE; LYSINE; or PROLINE. This enzyme requires ZINC as a cofactor and was formerly listed as EC 3.4.2.1 and EC 3.4.12.2.
A condition in which there is a change of one adult cell type to another similar adult cell type.
A bile salt formed in the liver by conjugation of chenodeoxycholate with taurine, usually as the sodium salt. It acts as detergent to solubilize fats in the small intestine and is itself absorbed. It is used as a cholagogue and choleretic.
A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.
The fluid inside CELLS.
Inorganic salts that contain the -HCO3 radical. They are an important factor in determining the pH of the blood and the concentration of bicarbonate ions is regulated by the kidney. Levels in the blood are an index of the alkali reserve or buffering capacity.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
The ability of a substrate to allow the passage of ELECTRONS.
The increase in a measurable parameter of a PHYSIOLOGICAL PROCESS, including cellular, microbial, and plant; immunological, cardiovascular, respiratory, reproductive, urinary, digestive, neural, musculoskeletal, ocular, and skin physiological processes; or METABOLIC PROCESS, including enzymatic and other pharmacological processes, by a drug or other chemical.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
A serine endopeptidase secreted by the pancreas as its zymogen, CHYMOTRYPSINOGEN and carried in the pancreatic juice to the duodenum where it is activated by TRYPSIN. It selectively cleaves aromatic amino acids on the carboxyl side.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
The craniosacral division of the autonomic nervous system. The cell bodies of the parasympathetic preganglionic fibers are in brain stem nuclei and in the sacral spinal cord. They synapse in cranial autonomic ganglia or in terminal ganglia near target organs. The parasympathetic nervous system generally acts to conserve resources and restore homeostasis, often with effects reciprocal to the sympathetic nervous system.
Cation-transporting proteins that utilize the energy of ATP hydrolysis for the transport of CALCIUM. They differ from CALCIUM CHANNELS which allow calcium to pass through a membrane without the use of energy.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
A subclass of symporters that specifically transport SODIUM CHLORIDE and/or POTASSIUM CHLORIDE across cellular membranes in a tightly coupled process.
A quaternary ammonium parasympathomimetic agent with the muscarinic actions of ACETYLCHOLINE. It is hydrolyzed by ACETYLCHOLINESTERASE at a considerably slower rate than ACETYLCHOLINE and is more resistant to hydrolysis by nonspecific CHOLINESTERASES so that its actions are more prolonged. It is used as a parasympathomimetic bronchoconstrictor agent and as a diagnostic aid for bronchial asthma. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1116)
A group of compounds with the heterocyclic ring structure of benzo(c)pyridine. The ring structure is characteristic of the group of opium alkaloids such as papaverine. (From Stedman, 25th ed)
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Insects of the order Dictyoptera comprising several families including Blaberidae, BLATTELLIDAE, Blattidae (containing the American cockroach PERIPLANETA americana), Cryptocercidae, and Polyphagidae.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Exotoxins produced by certain strains of streptococci, particularly those of group A (STREPTOCOCCUS PYOGENES), that cause HEMOLYSIS.
A family of DNA-binding transcription factors that contain a basic HELIX-LOOP-HELIX MOTIF.
A purinergic P2X neurotransmitter receptor that plays a role in pain sensation signaling and regulation of inflammatory processes.
Chemical bond cleavage reactions resulting from absorption of radiant energy.
The movement of ions across energy-transducing cell membranes. Transport can be active, passive or facilitated. Ions may travel by themselves (uniport), or as a group of two or more ions in the same (symport) or opposite (antiport) directions.
A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition.
Compounds that contain a BENZENE ring fused to a furan ring.
An inorganic dye used in microscopy for differential staining and as a diagnostic reagent. In research this compound is used to study changes in cytoplasmic concentrations of calcium. Ruthenium red inhibits calcium transport through membrane channels.
A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.
A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)
An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.
A colorless, syrupy, strongly acidic liquid that can form detergents with oleic acid.
An ionophorous, polyether antibiotic from Streptomyces chartreusensis. It binds and transports CALCIUM and other divalent cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. The substance is used mostly as a biochemical tool to study the role of divalent cations in various biological systems.
A ZINC-dependent carboxypeptidase primary found in the DIGESTIVE SYSTEM. The enzyme catalyzes the preferential cleavage of a C-terminal peptidyl-L-lysine or arginine. It was formerly classified as EC 3.4.2.2 and EC 3.4.12.3.
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
A plasma membrane exchange glycoprotein transporter that functions in intracellular pH regulation, cell volume regulation, and cellular response to many different hormones and mitogens.
Potent activator of the adenylate cyclase system and the biosynthesis of cyclic AMP. From the plant COLEUS FORSKOHLII. Has antihypertensive, positive inotropic, platelet aggregation inhibitory, and smooth muscle relaxant activities; also lowers intraocular pressure and promotes release of hormones from the pituitary gland.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
A methylpyrrole-carboxylate from RYANIA that disrupts the RYANODINE RECEPTOR CALCIUM RELEASE CHANNEL to modify CALCIUM release from SARCOPLASMIC RETICULUM resulting in alteration of MUSCLE CONTRACTION. It was previously used in INSECTICIDES. It is used experimentally in conjunction with THAPSIGARGIN and other inhibitors of CALCIUM ATPASE uptake of calcium into SARCOPLASMIC RETICULUM.
A lysosomal cysteine proteinase with a specificity similar to that of PAPAIN. The enzyme is present in a variety of tissues and is important in many physiological and pathological processes. In pathology, cathepsin B has been found to be involved in DEMYELINATION; EMPHYSEMA; RHEUMATOID ARTHRITIS, and NEOPLASM INVASIVENESS.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A family of gastrointestinal peptide hormones that excite the secretion of GASTRIC JUICE. They may also occur in the central nervous system where they are presumed to be neurotransmitters.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
A slowly hydrolyzed muscarinic agonist with no nicotinic effects. Pilocarpine is used as a miotic and in the treatment of glaucoma.
A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.
Ubiquitous, inducible, nuclear transcriptional activator that binds to enhancer elements in many different cell types and is activated by pathogenic stimuli. The NF-kappa B complex is a heterodimer composed of two DNA-binding subunits: NF-kappa B1 and relA.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
L-Tryptophyl-L-methionyl-L-aspartyl-L-phenylalaninamide. The C-terminal tetrapeptide of gastrin. It is the smallest peptide fragment of gastrin which has the same physiological and pharmacological activity as gastrin.
The measurement of an organ in volume, mass, or heaviness.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed)
An ester formed between the aldehydic carbon of RIBOSE and the terminal phosphate of ADENOSINE DIPHOSPHATE. It is produced by the hydrolysis of nicotinamide-adenine dinucleotide (NAD) by a variety of enzymes, some of which transfer an ADP-ribosyl group to target proteins.
Agents that inhibit SODIUM-POTASSIUM-CHLORIDE SYMPORTERS which are concentrated in the thick ascending limb at the junction of the LOOP OF HENLE and KIDNEY TUBULES, DISTAL. They act as DIURETICS. Excess use is associated with HYPOKALEMIA and HYPERGLYCEMIA.
A drug that exerts an inhibitory effect on gastric secretion and reduces gastrointestinal motility. It is used clinically in the drug therapy of gastrointestinal ulcers.
A synthetic pentapeptide that has effects like gastrin when given parenterally. It stimulates the secretion of gastric acid, pepsin, and intrinsic factor, and has been used as a diagnostic aid.
A type of glycoside widely distributed in plants. Each consists of a sapogenin as the aglycone moiety, and a sugar. The sapogenin may be a steroid or a triterpene and the sugar may be glucose, galactose, a pentose, or a methylpentose.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
A slowly hydrolyzing muscarinic agonist with no nicotinic effects. Bethanechol is generally used to increase smooth muscle tone, as in the GI tract following abdominal surgery or in urinary retention in the absence of obstruction. It may cause hypotension, HEART RATE changes, and BRONCHIAL SPASM.
A type of pancreatic cell representing about 50-80% of the islet cells. Beta cells secrete INSULIN.
Compounds with a core of fused benzo-pyran rings.
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)

Recovery of INS-R and ER-alpha expression in the salivary glands of diabetic mice submitted to hormone replacement therapy. (1/157)

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Histopathology and ex vivo insulin secretion of pancreatic islets in gestational diabetes: A case report. (2/157)

Gestational diabetes (GD) results from insufficient endogenous insulin supply. No information is available on features of islet cells in human GD. Herein, we describe several properties of islets from a woman with GD. Immunohistochemical stainings and EM analyses were performed on pancreatic samples. Islet isolation was achieved by enzymatic dissociation and density gradient centrifugation. Ex vivo insulin secretion was studied in response to fuel secretagogues. Control islets were obtained from matched non-pregnant, non-diabetic women. Total insulin positive area was lower in GD, mainly due to the presence of smaller islets. beta-cell apoptosis and the presence of Ki67 positive islet cells were similar in GD and controls, whereas the amount of insulin positive cells in or close to the ducts was decreased in GD. Ex vivo insulin secretion did not differ between GD and non-pregnant, non-diabetic islets. These findings suggest that in this case of human GD there might mainly be a defect of beta-cell amount, not due to increased apoptosis, but possibly to insufficient regeneration.  (+info)

Tauroursodeoxycholic acid reduces endoplasmic reticulum stress, acinar cell damage, and systemic inflammation in acute pancreatitis. (3/157)

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PKCtheta activation in pancreatic acinar cells by gastrointestinal hormones/neurotransmitters and growth factors is needed for stimulation of numerous important cellular signaling cascades. (4/157)

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Role of neutrophils in the activation of trypsinogen in severe acute pancreatitis. (5/157)

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Functional role of an islet transcription factor, INSM1/IA-1, on pancreatic acinar cell trans-differentiation. (6/157)

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Secretin is not necessary for exocrine pancreatic development and growth in mice. (7/157)

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The distribution of phosphatidylinositol 4,5-bisphosphate in acinar cells of rat pancreas revealed with the freeze-fracture replica labeling method. (8/157)

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TY - JOUR. T1 - Matrix metalloproteinase-7 is expressed by pancreatic cancer precursors and regulates acinar-to-ductal metaplasia in exocrine pancreas. AU - Crawford, Howard C.. AU - Scoggins, Charles R.. AU - Kay Washington, M.. AU - Matrisian, Lynn M.. AU - Leach, Steven D.. PY - 2002. Y1 - 2002. N2 - In gastrointestinal epithelium, metaplastic conversion between predominant cell types is associated with an increased risk of neoplasia. However, the mechanisms regulating metaplastic transitions in adult epithelia are largely undefined. Here we show that matrix metalloproteinase-7 (MMP-7) is expressed not only in the majority of human pancreatic ductal adenocarcinoma specimens, but also in human pancreatic intraepithelial neoplasia and metaplastic duct lesions in human and mouse. In a mouse model of pancreatic acinar-to-ductal metaplasia, MMP-7 progressively accumulates during the metaplastic transition, resulting in a concomitant increase in solubilization of Fas ligand (FasL). Under identical ...
Definition of acinar cell in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is acinar cell? Meaning of acinar cell as a legal term. What does acinar cell mean in law?
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TY - JOUR. T1 - Cancer-associated acinar-to-ductal metaplasia within the invasive front of pancreatic cancer contributes to local invasion. AU - Kibe, Shin. AU - Ohuchida, Kenoki. AU - Ando, Yohei. AU - Takesue, Shin. AU - Nakayama, Hiromichi. AU - Abe, Toshiya. AU - Endo, Sho. AU - Koikawa, Kazuhiro. AU - Okumura, Takashi. AU - Iwamoto, Chika. AU - Shindo, Koji. AU - Moriyama, Taiki. AU - Nakata, Kohei. AU - Miyasaka, Yoshihiro. AU - Shimamoto, Masaya. AU - Ohtsuka, Takao. AU - Mizumoto, Kazuhiro. AU - Oda, Yoshinao. AU - Nakamura, Masafumi. N1 - Funding Information: This work was supported in part by JSPS KAKENHI (Grant Numbers JP16H05418, JP16K10601, JP17H04284, JP17K19602, JP17K19605, JP18K07943).The authors thank E. Manabe, S. Sadatomi, N. Torada (Department of Surgery and Oncology, Kyushu University Hospital), and members of the Research Support Center and Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University for their expert technical assistance. The ...
Cancer subtype switching, which involves unclear cancer cell origin, cell fate decision, and transdifferentiation of cells within a confined tumor microenvironment, remains a major problem in pancreatic cancer (PDA). By analyzing PDA subtypes in The Cancer Genome Atlas, we identified that epigenetic silencing of apoptosis-associated tyrosine kinase (AATK) inversely was correlated with mRNA expression and was enriched in the quasi-mesenchymal cancer subtype. By comparing early mouse pancreatic lesions, the non-invasive regions showed AATK co-expression in cells with acinar-to-ductal metaplasia, nuclear VAV1 localization, and cell cycle suppression; but the invasive lesions conversely revealed diminished AATK expression in those with poorly differentiated histology, cytosolic VAV1 localization, and co-expression of p63 and HNF1α. Transiently activated AATK initiates acinar differentiation into a ductal cell fate to establish apical-basal polarization in acinar-to-ductal metaplasia. Silenced AATK and
Define acinar cell, acinous cell. acinar cell, acinous cell synonyms, acinar cell, acinous cell pronunciation, acinar cell, acinous cell translation, English dictionary definition of acinar cell, acinous cell. a small room as in a convent or prison; basic structural unit of all organisms: a one-celled animal Not to be confused with: sell - to transfer goods or...
https://doi.org/10.18632/oncotarget.2041 Parama Dey, Satyanarayana Rachagani, Arokia P. Vaz, Moorthy P. Ponnusamy, Surinder K. Batra
TY - JOUR. T1 - Pancreatic acinar cell physiology and function. AU - Miller, L. J.. PY - 1992/1/1. Y1 - 1992/1/1. N2 - The past year has witnessed a marked increase in the complexity of intracellular processes involved in the function of a polarized epithelial cell. The pancreatic acinar cell is perhaps the best studied such cell, and the classical model for stimulus-secretion coupling and secretory protein biosynthesis and export. In this review, I examine several new reports that impact on these themes. I include the identification of newly recognized pancreatic secretagogues; insights into the biochemical basis for hormone receptor affinity states and interconversion, desensitization, and internalization; lipid mediators of secretagogue action; patterns and regulators of intracellular calcium events along with their spatial and temporal patterns; regulated channels, transporters, and junctional communication in the acinar cell and cellular organelles; and new insights into regulated ...
B. DNA-Protein Cross-Linking. To cross-link protein to the genomic DNA, 270 µl of formalin was added to 10 ml of DMEM containing acini to obtain a final solution of 1% formaldehyde. Cells were agitated for 10 minutes (Note 1) on a shaker table. Following the addition of 514 µl of 2.5 M glycine (125 mM final) for 5 minutes to quench the formaldehyde and terminate the crosslinking reaction, cells were centrifuged at 2000 rpm for 3 minutes at 40C. The pellet of acinar cells was washed twice with ice cold PBS.. C. Isolation of Nuclei from Isolated Acinar Cells. To isolate nuclei, acinar cells were incubated in Cytoplasmic Extract buffer (CE Buffer; 10 mM HEPES pH 7.4, 10 mM KCl, 1.5 mM MgCl2, 0.1% Triton-X100, 0.5 mM DTT and protease inhibitors [5 µg/mL Aprotinin, 5 µg/mL Leupeptin, 5 µg/mL Pepstatin, 75 µg/mL PMSF]) followed by 5 strokes of Potter-Elvehjem homogenizer. The cells were set on ice for 15 minutes. To pellet the nuclei, the samples were centrifuged at 4oC and 5000 g for 10 ...
title: Suppression of NF-kappaB activation and cytokine production by N-acetylcysteine in pancreatic acinar cells., doi: 10.1016/S0891-5849(00)00368-3, category: Article
As multiple recent studies have described acinar cells undergoing acinar to ductal metaplasia as a common cell of origin of PDAC (and reviewed in the study by Rooman and Real) the central role of MYC in acinar cells within the pancreas is of particular ...
Interpretive Summary: The establishment and initial characterization of a pig embryonic stem cell-derived pancreatic cell line, PICM-31, is described. The PICM-31 cell line was started from the in vitro culture of the inner cells of an early pig embryo. The cells were small and grew relatively slowly. They frequently self-organized into radially symmetrical groupings of cells, i.e., wheel-like and similar to the acinar structures inside the pancreas. Electron microscopic examination confirmed that the cells were organized into a functional acinar-like unit and that they contained secretory granules as expected of the cells of the pancreas that secrete digestive enzymes. The PICM-31 cells were shown to be expressing the genes for digestive enzymes, and the secreted enzymes themselves were also identified by mass spectroscopy. The PICM-31 cells also expressed marker genes that indicated the cells may be progenitor pancreas cells, i.e., that the PICM-31 cells might be able to change into the ...
To protect acinar cells from self-digestion, the powerful digestive enzymes that the cells make are initially produced in an inactive form, notes Pancreas.org. These inert enzymes are converted into...
Differentiation of salisphere into acinar cells. (A) Amylase expressing cells (AC) in submandibular gland tissue (Tissue) were also present at the onset of cult
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Agonist-specific cytosolic Ca2+ oscillation patterns can be observed in individual cells and these have been explained by the co-existence of separate oscillatory mechanisms. In pancreatic acinar cells activation of muscarinic receptors typically evokes sinusoidal oscillations whereas stimulation of cholecystokinin (CCK) receptors evokes transient oscillations consisting of Ca2+ waves with long intervals between them. We have monitored changes in the cytosolic Ca2+ concentration ([Ca2+]i) by measuring Ca2(+)-activated Cl- currents in single internally perfused mouse pancreatic acinar cells. With minimal intracellular Ca2+ buffering we found that low concentrations of both ACh (50 nM) and CCK (10 pM) evoked repetitive short-lasting Ca2+ spikes of the same duration and frequency, but the probability of a spike being followed by a longer and larger Ca2+ wave was low for ACh and high for CCK. The probability that the receptor-evoked shortlasting Ca2+ spikes would initiate more substantial Ca2+ waves was
2. Activation of ERK pathway in Pancreatic Acinar Cells (Figure 1). ERK activation is usually monitored by following the dual phosphorylation of the Thr and Tyr residues in the Thr-Glu-Tyr activation sequence brought about by MEK as there are a number of good phosphospecific antibodies directed at this epitope. It can also be shown by phosphorylation of myelin basic protein either in a test tube or by an in gel technique following gel electrophoresis and renaturization. Both Western blots and the in gel kinase procedure reveal the two forms of ERK at approximately 44 and 42 kDa; in fact, the molecules were originally referred to as p42 and p44 MAPK with p42 being what is now referred to as ERK2 and p44 now being ERK1. Using isolated rat or mouse pancreatic acini in vitro, ERK1/2 is activated by CCK, bombesin, substance P, and carbachol, all of which activate G protein coupled receptors coupled to Gq and calcium mobilization but not by secretin or VIP which activate receptors coupled to Gs and ...
Pieces of mouse embryonic pancreatic epithelium cultured in an inductive situation in vitro, or when examined at critical times in vivo, show a gradient of zymogen granule accumulation. Cells located internally in explants, or in central acini in vivo, show this overt differentiation first. As the epithelia age, the more peripheral cell population proceeds in a similar differentiation. Observations of autoradiograms of H3-thymidine-labeled tissues indicate that the first cells which cease incorporating the DNA-precursor are in the central regions that differentiate first. In older explants, thymidine incorporation is largely restricted to the periphery of the tissue as zymogen appears in the internal cells. Evidence suggests that cells or nuclei which have replicated DNA move inward before dividing. Some daughter cells apparently return peripherad to divide again, whereas others remain centrally where they undergo differentiation. During at least the first 24 hours of these maturational changes, ...
With the exception of a few types of cells, (acinar cells, T lymphocytes and hepatocytes), every cell in your body has a cilia. In the vision community, we are used to seeing these structures in the distal portion of the photoreceptors. The reality is that every cell in the retina has a cilium and some cells […]. ...
TY - JOUR. T1 - Genome-wide genetic and epigenetic analyses of pancreatic acinar cell carcinomas reveal aberrations in genome stability. AU - Jäkel, Cornelia. AU - Bergmann, Frank. AU - Toth, Reka. AU - Assenov, Yassen. AU - Van Der Duin, Daniel. AU - Strobel, Oliver. AU - Hank, Thomas. AU - Klöppel, Günter. AU - Dorrell, Craig. AU - Grompe, Markus. AU - Moss, Joshua. AU - Dor, Yuval. AU - Schirmacher, Peter. AU - Plass, Christoph. AU - Popanda, Odilia. AU - Schmezer, Peter. PY - 2017/12/1. Y1 - 2017/12/1. N2 - Pancreatic acinar cell carcinoma (ACC) is an aggressive exocrine tumor with largely unknown biology. Here, to identify potential targets for personalized treatment, we perform integrative genome-wide and epigenome-wide analyses. The results show frequently aberrant DNA methylation, abundant chromosomal amplifications and deletions, and mutational signatures suggesting defective DNA repair. In contrast to pancreatic ductal adenocarcinoma, no recurrent point mutations are detected. The ...
RESULTS: We defined three distinctive phases-termed inflammation, regeneration and refinement-following induction of moderate acute pancreatitis in wild-type mice. These corresponded to different waves of proliferation of mesenchymal, progenitor-like and acinar cells. Pancreas regeneration required a coordinated transition of proliferation between progenitor-like and acinar cells. In mice harbouring an oncogenic Kras mutation and challenged with pancreatitis, there was an extended inflammatory phase and a parallel, continuous proliferation of mesenchymal, progenitor-like and acinar cells. Analysis of high-resolution transcriptional data from wild-type animals revealed that organ regeneration relied on a complex interaction of a gene network that normally governs acinar cell homeostasis, exocrine specification and intercellular signalling. In mice with oncogenic Kras, a specific carcinogenic signature was found, which was preserved in full-blown mouse pancreas cancer ...
TY - JOUR. T1 - NFATc4 Regulates Sox9 gene expression in acinar cell plasticity and pancreatic cancer initiation. AU - Hessmann, Elisabeth. AU - Zhang, Jin San. AU - Chen, Nai Ming. AU - Hasselluhn, Marie. AU - Liou, Geou Yarh. AU - Storz, Peter. AU - Ellenrieder, Volker. AU - Billadeau, Daniel D.. AU - Koenig, Alexander. N1 - Publisher Copyright: © 2016 Elisabeth Hessmann et al.. PY - 2016. Y1 - 2016. N2 - Acinar transdifferentiation toward a duct-like phenotype constitutes the defining response of acinar cells to external stress signals and is considered to be the initial step in pancreatic carcinogenesis. Despite the requirement for oncogenic Kras in pancreatic cancer (PDAC) development, oncogenic Kras is not sufficient to drive pancreatic carcinogenesis beyond the level of premalignancy. Instead, secondary events, such as inflammation-induced signaling activation of the epidermal growth factor (EGFR) or induction of Sox9 expression, are required for tumor formation. Herein, we aimed to ...
In the pig parotid gland, ATPDase was mainly associated with duct epithelium, with very little, if any, immunoreactivity on acinar cells. Myoepithelial cells dispersed among acini produced a significant signal. There have been reports of Ca2+-dependent ATPases associated with isolated parotid acinar cells, but as judged by their biochemical properties, these ATPases are different from ATPDases. However, a nucleoside triphosphatase described by Sato et al. (29) in bovine parotid gland and an apyrase described by Valenzuela et al. (34) in a microsomal fraction of rat salivary gland appear to correspond to the ATPDase described in this study and hence could well be associated with ductal epithelial and myoepithelial cells. In agreement with these findings, some studies on rat parotid acini show very little ecto-ATPase activity (9). Some P2Zpurinoceptors have been described on rat parotid acinar cells, which respond to ATP and mediate a Ca2+increase caused by both an influx and a mobilization from ...
We hypothesize, due to the Sjögrens syndrome (SS) disease characteristics, that the primary target hit by the disease process is the secretory acinar cell and that this cell is particularly in women damaged due to insufficient support, normally provided by dehydroepiandrosterone and its intracrine processing. Dehydroepiandrosterone deficiency at the time of adrenopause seems to us as the more likely endocrine trigger than estrogen deficiency caused by menopause as androgens in general are considered to be protective against autoimmunity and estrogens to favor it. Acinar cell is normally responsible for the production of primary saliva. Acinar cell damage can lead to acinar cell apoptosis and loss. Normally this is compensated by division of the acinar cells in situ or, according to recent reports, perhaps rather by division and subsequent migration of one of the daughter cells into the acinar space and transdifferentiation of this intercalated ductal cell progenitor into mature acinar cell. In ...
Foods good for pancreas : What is Pancreatic Inflammation? Diet suitable for inflammation of the pancreas.Worldwide, more than 10,000..
The stress-activated protein kinase (SAPK) p38 can induce apoptosis, and its inhibition facilitates mammary tumorigenesis. g38 signaling forms regular mammary acinar morphogenesis and inhibits HER2/neu-driven tumorigenesis. Our data also define at what stage of mammary gland advancement g38 might action to suppress tumorigenesis and how its inhibition could speed up disease development. Outcomes Reduction of ECM connection activates MKK6-g38 signaling and anoikis in MCF-10A cells Integrin and development aspect signaling become uncoupled in MCF-10A cells harvested in suspension system, starting a tension indication that outcomes in cell loss of life (19). Consistent with prior research (7), immunoblot (IB) evaluation indicated that g38 phosphorylation was MK-2866 elevated in separate MCF-10A cells essential contraindications to that in attached cells (Fig. 1A). We also noticed account activation of g38 in principal mouse MECs (mMECs) and immortalized mouse embryonic fibroblasts (MEFs) harvested ...
A malignant tumor arising from secreting cells of a racemose gland, particularly the salivary glands. Racemose (Latin racemosus, full of clusters) refers, as does acinar (Latin acinus, grape), to small saclike dilatations in various glands. Acinar cell carcinomas are usually well differentiated and account for about 13% of the cancers arising in the parotid gland. Lymph node metastasis occurs in about 16% of cases. Local recurrences and distant metastases many years after treatment are common. This tumor appears in all age groups and is most common in women. (Stedman, 25th ed; Holland et al., Cancer Medicine, 3d ed, p1240; from DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, p575 ...
The present study demonstrates that p8 is expressed in human islets and certain rodent pancreatic β-cell lines. Furthermore, proliferation and p8 expression in rat INS-1 β-cells is strongly inducible by high glucose concentrations in the medium. Our findings point to a possible new role of p8 as a mediator of glucose-induced pancreatic β-cell proliferation. This hypothesis is supported by studies demonstrating increased cell growth in p8 overexpressing rat AR42J pancreatic acinar cells (6) and human HeLa cervix epithelial cells (7). Moreover, glucose-induced pancreatic β-cell proliferation involves activation of PKC (2,3), and rat as well as human p8 amino acid sequences contain a highly conserved region with two PKC sites (16).. p8 was originally postulated as a pancreatic emergency gene product, which is exclusively upregulated in pancreatic acinar cells during stress caused by acute pancreatitis (6). The emergency gene character was underlined by the strong increase of pancreatic p8 ...
In these experiments, TG was used to deplete Ca2+ stores in cells maintained in nominally Ca2+-free extracellular solution. Addition of Ca2+ to the extracellular solution resulted in SOCE-mediated increase in [Ca2+]c (cytosolic Ca2+ concentration) followed by formation of an elevated [Ca2+]c plateau (Figure 4). Addition of InsP3-producing secretagogue ACh reversibly decreased the amplitude of the plateau (Figure 4A, n=465). Using the Mn quench technique [20] we also observed a small (13±2%) but statistically significant inhibition of the influx by 300 nM ACh (Supplementary Figure S5 available at http://www.BiochemJ.org/bj/436/bj4360231add.htm, n=145 cells in ACh-treated group and n=139 cells in control group). Caffeine (10 mM), which in PACs very efficiently blocks InsP3-induced Ca2+ responses [21] has no effect on its own (Figure 4B, n=169), but it partially reversed the ACh-induced reduction of the plateau (Figure 4C, n=168). These experiments suggest that activation of IP3Rs has a mild ...
Della torre, G, Ultracytochemical characterization of c particle-producing vacuoles in pancreatic acinar cells of the mouse. (1974). Subject Strain Bibliography 1974. 1981 ...
GSK-7975A inhibits CRAC entry (Fura-2 340:380 normalized at 700 s). (A) Changes in mouse pancreatic acinar [Ca2+]C induced by CCK (1 nmol/L) with external physi
the expression of caspase-1 is a frequent event in chronic pancreatitis and its distribution pattern may reflect two functions of this protease: on one hand its participation in the apoptotic pathway in atrophic acinar cells and, on the other hand, its role in proliferation and differentiation in proliferating duct cells. PMID: 11345139 ...
Mouse polyclonal antibody raised against a full-length human ZSCAN23 protein. ZSCAN23 (ABZ92213.1, 1 a.a. ~ 389 a.a) full-length human protein. (H00222696-B01P) - Products - Abnova
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Regulated secretion from pancreatic acinar cells occurs by exocytosis of zymogen granules (ZG) at the apical plasmalemma. ZGs originate from the TGN and undergo prolonged maturation and condensation. After exocytosis, the zymogen granule membrane (ZGM) is retrieved from the plasma membrane and ultimately reaches the TGN. In this study, we analyzed the fate of a low M(r) GTP-binding protein during induced exocytosis and membrane retrieval using immunoblots as well as light and electron microscopic immunocytochemistry. This 27-kD protein, identified by a monoclonal antibody that recognizes rab3A and B, may be a novel rab3 isoform. In resting acinar cells, the rab3-like protein was detected primarily on the cytoplasmic face of ZGs, with little labeling of the Golgi complex and no significant labeling of the apical plasmalemma or any other intracellular membranes. Stimulation of pancreatic lobules in vitro by carbamylcholine for 15 min, resulted in massive exocytosis that led to a near doubling of ...
Despite expression of oncogenic KRAS, premalignant pancreatic intraepithelial neoplasia 1 (PanIN1) lesions rarely become fully malignant pancreatic ductal adenocarcinoma (PDAC). The molecular mechanisms through which established risk factors, such as chronic pancreatitis, acinar cell damage, and/or defective autophagy increase the likelihood of PDAC development are poorly understood. We show that accumulation of the autophagy substrate p62/SQSTM1 in stressed KrasG12D acinar cells is associated with PDAC development and maintenance of malignancy in human cells and mice. p62 accumulation promotes neoplastic progression by controlling the NRF2-mediated induction of MDM2, which acts through p53-dependent and -independent mechanisms to abrogate checkpoints that prevent conversion of differentiated acinar cells to proliferative ductal progenitors. MDM2 targeting may be useful for preventing PDAC development in high-risk individuals ...
The lacrimal gland (LG) is an exocrine gland important for secretion of the tear film. The kinase p38 has important signal transduction functions, e.g. in gene transcription, but has previously not been known to modulate exocrine secretion. The aim of the current study was to investigate the role of p38 in carbachol (Cch)-induced LG secretion in LG acinar cells in vitro. Western blotting was used to determine the phosphorylation status of p38 and p42/44 and determine expression of p38 isoforms. To determine the effect of p38 inhibition on LG secretion, PD 169316, a general p38 inhibitor, and SB 239063, an inhibitor of p38α and β, were added to the cells prior to secretion measurements. The results revealed activation of p38 mediated by Cch stimulation and inhibition of Cch-induced secretion as a result of p38 inhibition. The inhibition was observed with PD 169316 isoforms, but not with SB 239063. The p38δ isoform was shown to have robust expression both by Western blotting of acinar cells and ...
Results Non-neoplastic acinar cells were stained diffusely, but epithelial cells of the pancreatic duct and the islets of Langerhans were not stained. In pancreatic tumours, all the seven ACCs were diffusely positive for the 2P-1-2-1 antibody. However, no positive staining was found in other pancreatic tumours including NETs, SPNs and ductal adenocarcinomas. The sensitivity and specificity of the 2P-1-2-1 antibody for ACCs were both 100%. In other organs studied, positive staining was observed only in the ectopic pancreas. ...
Acute pancreatitis (AP), a human disease in which the pancreas digests itself, has substantial mortality with no specific therapy. The major causes of AP are alcohol abuse and gallstone complications, but it also occurs as an important side effect of the standard asparaginase-based therapy for childhood acute lymphoblastic leukemia. Previous investigations into the mechanisms underlying pancreatic acinar cell death induced by alcohol metabolites, bile acids, or asparaginase indicated that loss of intracellular ATP generation is an important factor. We now report that, in isolated mouse pancreatic acinar cells or cell clusters, removal of extracellular glucose had little effect on this ATP loss, suggesting that glucose metabolism was severely inhibited under these conditions. Surprisingly, we show that replacing glucose with galactose prevented or markedly reduced the loss of ATP and any subsequent necrosis. Addition of pyruvate had a similar protective effect. We also studied the effect of ...
The splicing-factor oncoprotein SRSF1 (also known as SF2/ASF or ASF/SF2) is upregulated in breast cancers. We investigated the ability of SRSF1 to transform human and mouse mammary epithelial cells in vivo and in vitro. SRSF1-overexpressing COMMA-1D cells formed tumors, following orthotopic transplantation to reconstitute the mammary gland. In three-dimensional (3D) culture, SRSF1-overexpressing MCF-10A cells formed larger acini than control cells, reflecting increased proliferation and delayed apoptosis during acinar morphogenesis. These effects required the first RNA-recognition motif and nuclear functions of SRSF1. SRSF1 overexpression promoted alternative splicing of BIM (also known as BCL2L11) and BIN1 to produce isoforms that lack pro-apoptotic functions and contribute to the phenotype. Finally, SRSF1 cooperated specifically with MYC to transform mammary epithelial cells, in part by potentiating eIF4E activation, and these cooperating oncogenes are significantly coexpressed in human breast ...
Pancreatic ductal adenocarcinoma (PDAC) is the third leading cause of cancer death in North America. The highest risk factor for PDAC is recurrent pancreatitis. While the link between PDAC and pancreatitis is unknown, de-differentiation of acinar cells is common to both diseases. Our lab has shown that Activating Transcription Factor 3 (ATF3), a factor upregulated during pancreatic injury, contributes to the development of acinar-to-ductal cell metaplasia (ADM), a precursor phenotype of PDAC. The goal of this study was to identify how ATF3 contributes to ADM. I hypothesize that ATF3 regulates acinar gene expression promoting ADM. We observed decreased ADM development in Atf3-/- acinar cultures, along with expression changes in differentiation genes and ADM promoting pathways (EGFR) in vivo. Assessment following chronic injury indicated absence of ATF3 resulted in decreased tissue damage. These results suggest a novel mechanism where ATF3 promotes ADM through loss of the mature acinar cell phenotype.
The role of tuberous sclerosis complex (TSC) in the pathogenesis of pancreatic cancers remains largely unknown. The present study shows that neurogenin 3 directed Cre deletion of Tsc1 gene induces the development of pancreatic acinar carcinoma. By cross-breeding the Neurog3-cre mice with Tsc1(loxp/loxp) mice, we generated the Neurog3-Tsc1-/- transgenic mice in which Tsc1 gene is deleted and mTOR signaling activated in the pancreatic progenitor cells. All Neurog3-Tsc1-/- mice developed notable adenocarcinoma-like lesions in pancreas starting from the age of 100 days old. The tumor lesions are composed of cells with morphological and molecular resemblance to acinar cells. Metastasis of neoplasm to liver and lung was detected in 5% of animals. Inhibition of mTOR signaling by rapamycin significantly attenuated the growth of the neoplasm. Relapse of the neoplasm occurred within 14 days upon cessation of rapamycin treatment. Our studies indicate that activation of mTOR signaling in the pancreatic ...
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Secretory granules contain specific proteins and other macromolecules that are destined for secretion into the extracellular space. This slide shows secretory granules in pancreatic acinar cells. Their size is approximately 1 µm in diameter, and they accumulate on the apical side of the cell above the nucleus. ...
TY - GEN. T1 - Role of Vascular Endothelial Growth Factor-D (VEGF-D) on IL-6 expression in cerulein-stimulated pancreatic acinar cells. AU - Lee, Jangwon. AU - Kyung, Hwan Kim. AU - Kim, Hyeyoung. PY - 2007/1. Y1 - 2007/1. N2 - Cerulein pancreatitis is similar to human edematous pancreatitis with dysregulation of the digestive enzyme production and cytoplasmic vacuolization, the death of acinar cells, edema formation, and an infiltration of inflammatory cells into the pancreas. Our preliminary proteomic analysis showed increased expression of vascular endothelial growth factor-D (VEGF-D) in cerulein (10 -8M)-treated pancreatic acinar cells. We hypothesized that VEGF-D may have a role in cerulein-induced cytokine expression in pancreatic acinar cells. This article was designed to elucidate whether cerulein induces VEGF-D expression in pancreatic AR42J cells, and whether VEGF-D expression is related to interleukin-6 (IL-6) expression by transfecting antisense oligonucleotide (AS ODN) for VEGF-D ...
The molecular identities of the pathways that mediate K+ and Cl− effluxes in serous acinar cells are unknown. CFTR mRNA is expressed in these cells, and immunocytochemistry revealed it to be present near the apical membrane. To examine the role of CFTR in Ca2+-evoked fluid secretion, we employed cells from the cftrtm1Unc−/− mouse and we pharmacologically inhibited CFTR in WT mouse cells. The results demonstrated that mouse nasal acinar cells from both WT and cftrtm1Unc−/− mice, as well as WT cells treated with CFTRinh172, all exhibited similar rates of Cl− efflux in response to muscarinic stimulation. Isolated intact submucosal glands from CF individuals that lack functional CFTR can secrete fluid in response to cholinergic agonists (Joo et al. 2002a), and it was recently shown that intact glands in WT and cftrtm1Unc−/− mice trachea secrete fluid in response to CCh at similar rates (Ianowski et al. 2007). It has remained unclear if cholinergic-induced fluid secretion in CF glands ...
The inability to visualize the initiation and progression of type-1 diabetes (T1D) noninvasively in humans is a major research and clinical stumbling block. We describe an advanced, exportable method for imaging the pancreatic inflammation underlying T1D, based on MRI of the clinically approved magnetic nanoparticle (MNP) ferumoxytol. The MNP-MRI approach, which reflects nanoparticle uptake by macrophages in the inflamed pancreatic lesion, has been validated extensively in mouse models of T1D and in a pilot human study. The methodological advances reported here were enabled by extensive optimization of image acquisition at 3T, as well as by the development of improved MRI registration and visualization technologies. A proof-of-principle study on patients recently diagnosed with T1D versus healthy controls yielded two major findings: First, there was a clear difference in whole-pancreas nanoparticle accumulation in patients and controls; second, the patients with T1D exhibited pronounced inter- ...
Morphological analysis of transplantable pancreatic acinar carcinoma of rat has revealed a continuum of cells from those which totally lack mature secretory granules to cells with abundant well-formed secretory granules. This tumor is easily dissociable into single cells which incorporate [3H]thymidine and [3H]leucine into DNA and protein at a linear rate for 3 to 4 hr. The number of cells synthesizing DNA, as assessed by light microscope autoradiography, was about 17%. Dissociated neoplastic pancreatic acinar cells are classified into four cell types (types I to IV) on the basis of nuclear morphology, degree of polarization, and the extent of specialization of cytoplasmic organelles. Type IV cells possess all the characteristics of mature pancreatic acinar cells, such as abundant rough endoplasmic reticulum, prominent Golgi apparatus, and numerous secretory granules. Cell types III, II, and I are progressively less differentiated. An analysis of the cytological features of 285 dissociated tumor ...
PI3K/Akt activation plays a critical role in the regeneration of pancreatic acini after resection. Furthermore, pancreatic regeneration is markedly attenuated in the aged pancreas most likely because of decreased PI3K/Akt activation.
The human body is in a constant race to replace cells, tissues, and whole organs that are lost due to injury, disease, and normal aging. Although the body has remarkable inherent regenerative capacity, it often cannot keep pace. Donor cells and tissues are available in limited quantities and are beset by problems such as immune compatibility and transplant rejection. Recently, technologies to convert one cell type into another, so-called direct reprogramming, have provided promise as a new source of cells for regenerative medicine. Li et al. now provide an enhanced method to directly reprogram native mouse pancreatic acinar cells into induced pancreatic beta cells that are functional and persist long-term.. A wave of recent studies have shown that cell identity can be readily modulated by the expression of key transcription factors. However, there remain significant uncertainties about whether the cells created by direct reprogramming will prove useful in regenerative medicine, as their ...
Adventitia and perivascular adipose tissue the integral unit in vascular disease (Chapter 20).. New! Arginase-II promotes tumor necrosis factor-α release from pancreatic acinar cells causing β-cell apoptosis in aging. ...
J:102698 Murtaugh LC, Law AC, Dor Y, Melton DA, Beta-catenin is essential for pancreatic acinar but not islet development. Development. 2005 Nov;132(21):4663-74 ...
Acinar cells make up 82% of the total pancreas; these cells are responsible for the production of the digestive enzymes. ... "Acinar Cell". Auckland Bioengineering Institute-University of Auckland. Archived from the original on 26 February 2008. ... Pancreatitis can result in exocrine pancreatic insufficiency, if the organ's acinar cells are permanently damaged; the ... either continued to exhibit inflammation of the organ or had decreased acinar cell function, even though they had no ...
Merocrine secretion - cells excrete their substances by exocytosis into a duct; for example, pancreatic acinar cells. Apocrine ... Serous cells secrete proteins, often enzymes. Examples include gastric chief cells and Paneth cells Mucous cells secrete mucus ... Holocrine secretion - the entire cell disintegrates to excrete its substance; for example, sebaceous glands of the skin and ... The glandular portion may be tubular or acinar, or may be a mix of the two (called tubuloacinar). If the glandular portion ...
There are cystic spaces surrounded by two uniform rows of epithelial cells with centrally placed pyknotic nuclei. The cystic ...
When used without context, it frequently refers to infections (squamous cell papilloma) caused by human papillomavirus (HPV), ...
"Acinar cell apoptosis in Serpini2-deficient mice models pancreatic insufficiency". PLOS Genetics. 1 (3): e38. doi:10.1371/ ... Cell. 129 (2): 263-75. doi:10.1016/j.cell.2007.02.042. PMID 17448989. Gooptu B, Dickens JA, Lomas DA (February 2014). "The ... Cell. 130 (6): 1108-19. doi:10.1016/j.cell.2007.07.013. PMC 2128786. PMID 17889653. Hejgaard J, Rasmussen SK, Brandt A, ... "Squamous cell carcinoma antigen 2 is a novel serpin that inhibits the chymotrypsin-like proteinases cathepsin G and mast cell ...
VAMP8 also participates the regulated exocytosis in pancreatic acinar cells. VAMP4 is involved in transport from the Golgi. ... Cell. 10 (6): 1957-72. doi:10.1091/mbc.10.6.1957. PMC 25394. PMID 10359608. Vesicle-Associated+Membrane+Protein+1 at the US ...
... "cAMP potentiates ATP-evoked calcium signaling in human parotid acinar cells". The Journal of Biological Chemistry. 279 (38): ... and in fat cells. P2X4 receptors have been implicated in the regulation of cardiac function, ATP-mediated cell death, synaptic ... Continued binding leads to increased permeability to N-methyl-D-glucamine (NMDG+) in about 50% of the cells expressing the P2X4 ... The P2X4 receptor has high calcium permeability, leading to the depolarization of the cell membrane and the activation of ...
"Ca2+ signalling and Ca2+-activated ion channels in exocrine acinar cells". Cell Calcium. 38 (3-4): 171-200. doi:10.1016/j.ceca. ... Signaling occurs when the cell is stimulated to release Ca2+ ions from intracellular stores, and/or when Ca2+ enters the cell ... Nanodomain European Calcium Society Clapham DE (December 2007). "Calcium signaling". Cell. 131 (6): 1047-58. doi:10.1016/j.cell ... Many cell surface receptors, including G protein-coupled receptors and receptor tyrosine kinases, activate the PLC enzyme. PLC ...
... is a malignant tumor of the delta cells of the endocrine pancreas that produces somatostatin. Increased levels ...
Cells resemble normal hepatocytes and are traversed by blood vessels but lack portal tracts or central veins. ... The hepatocytes are on a regular reticulin scaffold and less or equal to three cell thick. ... because of the risk of rupture causing bleeding and because they may contain malignant cells.[8] Current recommendations are ...
Microscopic appearance is signet ring cell carcinoma, which is tumor cells with mucin droplet that displaces the nucleus to one ... Diffuse stomach cancer is characterized by the presence of poorly differentiated tumor cells. ... rigid stomach wall caused by diffuse infiltration of tumor cells and extensive fibrosis. ... Acinar cell (8550-8559). *Acinic cell carcinoma. Other. Complex epithelial (8560-8589). *Warthin's tumor ...
When monkey lacrimal acinar cells are stressed with inflammatory cytokines (as occurs in dry eye), carbachol loses its capacity ... Cell surface proteoglycan syndecan-1 is partly responsible. Biotinylated cell surface proteins from a lacritin-responsive cell ... However, in secretion assays using monkey lacritin on monkey lacrimal acinar cells, the dose response appears to be sigmoidal ... In lacrimal gland, polarized lacrimal acinar cells appear to be the most prolific lacritin producers, as evidenced by strong ...
Parasympathetic stimulation leads to acetylcholine (ACh) release onto the salivary acinar cells. ACh binds to muscarinic ... In humans, saliva is 98% water plus electrolytes, mucus, white blood cells, epithelial cells (from which DNA can be extracted ... secreted by the acinar cells of the parotid and submandibular glands, starts the digestion of starch before the food is even ... which is secreted by the acinar cells of the sublingual gland; has a pH optimum around 4.0 so it is not activated until ...
5-trisphosphate receptors in parotid acinar cells. A mechanism for the synergistic effects of cAMP on Ca2+ signaling". J. Biol ... induced apoptosis of human T-lymphoma cells". Cell Calcium. 27 (6): 315-28. doi:10.1054/ceca.2000.0126. PMID 11013462. Mayne M ... Cell. Signal. 20 (4): 737-47. doi:10.1016/j.cellsig.2007.12.010. PMID 18249094. Wilker E, Mittleman MA, Litonjua AA, et al. ( ... Cell Biol. 183 (2): 297-311. doi:10.1083/jcb.200803172. PMC 2568025. PMID 18936250. Johnson JM, Castle J, Garrett-Engele P, et ...
"Subcellular distribution and function of Rab3A-D in pancreatic acinar AR42J cells". Biochem. Biophys. Res. Commun. 287 (3): 746 ... Weber E, Jilling T, Kirk KL (March 1996). "Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells". J. ... Cell. 96 (3): 363-74. doi:10.1016/S0092-8674(00)80549-8. PMID 10025402. S2CID 15162326. Jung YJ, Lee TH, Lee JY, Kim JH, Park ... Mol Cell Biol. 15 (3): 1137-43. doi:10.1128/MCB.15.3.1137. PMC 230335. PMID 7532276. "Entrez Gene: RAB3A RAB3A, member RAS ...
... chemical Bisphenol-A has been shown to lead to hyperprolactinaemia and growth of prolactin-producing pituitary cells.[5] The ... "Effects of xenoestrogen bisphenol A on uterine and pituitary weight, serum prolactin levels and immunoreactive prolactin cells ...
"Pancreatic ductal and acinar cell neoplasms in Carney complex: a possible new association". J Clin Endocrinol Metab. 96 (11): ... Testicular cancer, particularly Sertoli cell type, is associated with Carney syndrome. Thyroid and pancreas cancer may also ...
... other cell types have followed suit, as exemplified by the pancreas (acinar and beta cells), T-cells, and smooth muscle. Levels ... "Cell-permeant NAADP: a novel chemical tool enabling the study of Ca2+ signalling in intact cells". Cell Calcium. 43 (6): 531-8 ... A 2'-3'-phosphatase stimulated by Ca2+ has been proposed in brain and, possibly in pancreatic acinar cells, that catabolises ... For example, NAADP/TPCs are unique drivers of cell killing by Cytotoxic T cells. Similarly, Phagocytosis via the Fc receptor in ...
They co-localize with Orai channels (TC# 1.A.52) in pancreatic acinar cells. IP3 receptors possess three domains: N-terminal ... "InsP₃receptors and Orai channels in pancreatic acinar cells: co-localization and its consequences". The Biochemical Journal. ... They predominate in the endoplasmic reticular membranes of various cell types in the brain but have also been found in the ... Ry receptors occur primarily in muscle cell sarcoplasmic reticular (SR) membranes, and IP3 receptors occur primarily in brain ...
"Rab4 associates with the actin terminal web in developing rat pancreatic acinar cells". European Journal of Cell Biology. 72 (1 ... Molecular Biology of the Cell. 11 (7): 2201-11. doi:10.1091/mbc.11.7.2201. PMC 14913. PMID 10888662. Li L, Omata W, Kojima I, ... dephosphorylation determines the localization of rab4 during the cell cycle". The EMBO Journal. 11 (12): 4379-89. doi:10.1002/j ...
2003). "CRHSP-24 phosphorylation is regulated by multiple signaling pathways in pancreatic acinar cells". Am. J. Physiol. ... Kim JE, Tannenbaum SR, White FM (2005). "Global phosphoproteome of HT-29 human colon adenocarcinoma cells". J. Proteome Res. 4 ... doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. v t e. ... 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635-48. ...
In addition to promoting the inflammatory cell reaction to acinar cells, ceruletide induces pancreatitis through dysregulation ... "Cerulein upregulates ICAM-1 in pancreatic acinar cells, which mediates neutrophil adhesion to these cells". American Journal of ... Ceruletide upregulates pancreatic acinar cell intercellular adhesion molecule-1 (ICAM-1) proteins through intracellular ... Surface ICAM-1 in turn promotes neutrophil adhesion onto acinar cells enhancing pancreatic inflammation. ...
"Expression and localization of rab escort protein isoforms in parotid acinar cells from rat". Journal of Cellular Physiology. ... June 2004). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation ...
June 2002). "Loss of hyperpolarization-activated Cl(-) current in salivary acinar cells from Clcn2 knockout mice". The Journal ... Cell Physiology. 282 (4): C805-16. doi:10.1152/ajpcell.00291.2001. PMID 11880269. Nehrke K, Arreola J, Nguyen HV, Pilato J, ... Cell Physiology. 290 (1): C45-56. CiteSeerX 10.1.1.533.5601. doi:10.1152/ajpcell.00209.2005. PMID 16049054. CLCN2+protein,+ ... "Expression of voltage-gated chloride channels in human glioma cells". The Journal of Neuroscience. 23 (13): 5572-82. doi: ...
2003). "CRHSP-24 phosphorylation is regulated by multiple signaling pathways in pancreatic acinar cells". Am. J. Physiol. ... J Cell Mol Med. 7 (1): 35-42. doi:10.1111/j.1582-4934.2003.tb00200.x. PMC 6740078. PMID 12767259. "Entrez Gene: CSDC2 cold ...
Histologically, it forms clusters of goblet cells containing mucin with a minor admixture of Paneth cells and endocrine cells. ... Goblet cell carcinoid[edit]. Main article: Goblet cell carcinoid. This is considered to be a hybrid between an exocrine and ... Carcinoid (also carcinoid tumor) is a slow-growing[1] type of neuroendocrine tumor originating in the cells of the ... The term 'crypt cell carcinoma' has been used for them, and though perhaps more accurate than considering them carcinoids, has ...
ATPases in rat submandibular acinar cells". Cell Calcium. 43 (5): 469-481. doi:10.1016/j.ceca.2007.08.001. PMID 17889347. ... The number of mitochondria in a cell can vary widely by organism, tissue, and cell type. A mature red blood cell has no ... The bottleneck exploits stochastic processes in the cell to increase in the cell-to-cell variability in mutant load as an ... and cell death, as well as maintaining control of the cell cycle and cell growth. Mitochondrial biogenesis is in turn ...
... is also present in secondary granules of PMNs and is secreted by some acinar cells. Lactoferrin can be purified ... Many viruses tend to bind to the lipoproteins of the cell membranes and then penetrate into the cell. Lactoferrin binds to the ... Such an indirect antiviral effect is achieved by affecting natural killer cells, granulocytes and macrophages - cells, which ... Lactoferrin is one of the transferrin proteins that transfer iron to the cells and control the level of free iron in the blood ...
... lo Prostate Cancer Cell Population Harbors Self-Renewing Long-Term Tumor-Propagating Cells that Resist Castration". Cell Stem ... Micrograph of prostate adenocarcinoma, acinar type, the most common type of prostate cancer. Needle biopsy, H&E stain ... LNCaP cells express androgen receptor (AR), but PC-3 and DU-145 cells express very little or no AR. AR, an androgen-activated ... Prostate cancer cells are generally devoid of zinc. This allows prostate cancer cells to save energy not making citrate, and ...
In pancreatic acinar cells and the anterior pituitary, strong and specific presence of cytochrome-c was detected in zymogen ... Cytochrome c also has an intermediate role in apoptosis, a controlled form of cell death used to kill cells in the process of ... Waterhouse NJ, Trapani JA (Jul 2003). "A new quantitative assay for cytochrome c release in apoptotic cells". Cell Death and ... Cytochrome c is known to play a role in the electron transport chain and cell apoptosis. However, a recent study has shown that ...
8550-8559) Acinar cell neoplasms. *(8560-8580) Complex epithelial neoplasms. Carcinoma In situ[edit]. The term carcinoma in ... Non-hematopoietic mesenchymal cells ⇨ sarcoma. *Hematopoietic cells *Bone marrow-derived cells that normally mature in the ... Composed of large, monotonous rounded or overtly polygonal-shaped cells with abundant cytoplasm.. Small cell carcinoma. Cells ... Certain combinations of mutations in the given progenitor cell ultimately result in that cell (also called a cancer stem cell) ...
An alternative source of beta cells, such insulin-producing cells derived from adult stem cells or progenitor cells would ... Pancreatic islets, the lighter tissue among the darker, acinar pancreatic tissue, hemalum-eosin stain. ... activates beta cells and inhibits alpha cells. *Glycogen/Glucagon: activates alpha cells which activates beta cells and delta ... PP cells (gamma cells or F cells) producing pancreatic polypeptide (,5%). It has been recognized that the cytoarchitecture of ...
5-trisphosphate receptors in parotid acinar cells. A mechanism for the synergistic effects of cAMP on Ca2+ signaling.». J. Biol ... J. Cell Biol. 183 (2): 297-311. PMC 2568025. . PMID 18936250. doi:10.1083/jcb.200803172. !CS1 manut: Uso explícito de et al. ( ... induced apoptosis of human T-lymphoma cells.». Cell Calcium. 27 (6): 315-28. PMID 11013462. doi:10.1054/ceca.2000.0126. ... cell cortex. •membrane. •integral component of membrane. •platelet dense tubular network membrane. •sarcoplasmic reticulum ...
... in which cells within the sebaceous gland rupture and disintegrate as they release the sebum and the cell remnants are secreted ... The glands have an acinar structure (like a many-lobed berry), in which multiple glands branch off a central duct. The glands ... "Histochemistry and Cell Biology. 147 (6): 733-748. doi:10.1007/s00418-016-1535-3. PMID 28130590.. ... Relative to keratinocytes that make up the hair follicle, sebaceous glands are composed of huge cells with many large vesicles ...
... acinar cells, endocrine cells, and ductal cells. These progenitor cells are characterised by the co-expression of the ... beta cells, delta cells, and PP cells, each of which releases a different hormone. These cells have characteristic positions, ... α2: decreases secretion from beta cells, increases secretion from alpha cells, β2: increases secretion from beta cells. ... M3: increases stimulation of alpha cells and beta cells[17]. DigestionEdit. ...
Melvin JE, Yule D, Shuttleworth T, Begenisich T. REGULATION OF FLUID AND ELECTROLYTE SECRETION IN SALIVARY GLAND ACINAR CELLS. ...
Acinar(英语:Acinus) cell (8550-8559). *Acinic cell carcinoma(英语:Acinic cell carcinoma) ... 印戒细胞癌(Signet ring cell carcinoma,SRCC),又称黏液细胞癌(mucinous cell carcinoma,MCC)是上皮组织的恶性肿瘤,组织学外观特点是可以见到印戒细胞。 ... Early diagnosis of signet ring cell carcinoma of the stomach: role of the Genta stain. J. Clin. Pathol
The next most common type, acinar cell carcinoma of the pancreas, arises in the clusters of cells that produce these enzymes, ... The pancreas has many functions, served by the endocrine cells in the islets of Langerhans and the exocrine acinar cells. ... "islet cell cancers",[28] even though it is now known that they do not actually arise from islet cells as previously thought.[27 ... acinar cell carcinomas may cause over-production of certain molecules, in this case digestive enzymes, which may cause symptoms ...
"Mechanisms by which hypoxia augments Leydig cell viability and differentiated cell function in vitro". Digital Library and ... Islet-Acinar. Axis. Pancreas: glucagon · insulin · amylin · somatostatin · pancreatic polypeptide. Pineal gland ... Guldenaar SE, Pickering BT (1985). "Immunocytochemical evidence for the presence of oxytocin in rat testis". Cell Tissue Res. ... "The human vasopressin gene is linked to the oxytocin gene and is selectively expressed in a cultured lung cancer cell line". J ...
Exocrine pancreatic dysfunction: Pancreatic exocrine insufficiency arises due to a lack of acinar cells that produce digestive ... Anemia (low red blood cell counts) and thrombocytopenia (low platelet counts) may also occur. Bone marrow is typically ... platelets and red blood cells. Patients may also develop progressive marrow failure or transform to acute myelogenous leukemia. ...
... is also known to induce pancreatic acinar cell carcinomas in rats through an unknown mechanism, perhaps by ...
... parafollicular cells.[4] These cells secrete calcitonin and so are also called C cells.[16] ... that occur in the lower neck more proximal to the thyroid gland are lined by epithelium resembling the thyroidal acinar ... Follicular cells. The core of a follicle is surrounded by a single layer of follicular cells. When stimulated by thyroid ... This is an ion channel on the cell membrane which in the same action transports two sodium ions and an iodide ion into the cell ...
5-bisphosphate in acinar cells of rat pancreas revealed with the freeze-fracture replica labeling method". PLoS ONE. 6 (8): ... "eat-5 and unc-7 represent a multigene family in Caenorhabditis elegans involved in cell-cell coupling". J. Cell Biol. 134 (2): ... When cells are compromised due to disease or injury and start to die messages are transmitted to neighboring cells connected to ... Shibata, Y; Yamamoto, T (March 1977). "Gap junctions in the cardiac muscle cells of the lamprey". Cell Tissue Res. 178 (4): 477 ...
... which is then received by β-adrenergic receptors on the acinar and ductal cells of the salivary glands, leading to an increase ... Since iron is a major component of bacterial cell walls, removal of iron breaks down the cell wall, which in turn breaks down ... Secretory cells are found in a group, or acinus (plural, acini). Each acinus is located at the terminal part of the gland ... About 20,000 protein coding genes are expressed in human cells and 60% of these genes are expressed in normal, adult salivary ...
Atypical small acinar proliferation. B. *Banff Classification. *Bergmann gliosis. *Bethesda system. *Biochemistry of ... Solid cell nests. *Specimen provenance complications. *Steatosis. *Stereotactic biopsy. *Sudden arrhythmic death syndrome ...
第二常見的胰臟癌為發源於胰臟外分泌腺體細胞的胰臟腺泡細胞癌(英語:acinar cell carcinoma of the pancreas),佔胰臟外分泌癌症的5%,腺泡細胞會過度分泌特定的分子消化酶而導致如皮膚紅疹和關節痛等症狀[30]。 ... 胰臟內的腫瘤也可能來自身體的其他地方,但這種狀況很少見,僅佔所有胰臟腫瘤的2
... macrophages and dendritic cells.[4][5][6] This innate immune cell and lymphocyte infiltration can result in destruction of the ... insulitis and associated changes in the exocrine acinar tissue". Diabetologia. 26 (6): 456-61. doi:10.1007/bf00262221. PMID ... The islets containing the pancreatic β-cells, and in some cases, the exocrine tissues, become infiltrated by T and B ... "Insulitis and beta cell mass in the natural history of type 1 diabetes". Diabetes. 65 (3): 719-731. doi:10.2337/db15-0779. PMC ...
"Local in vivo GSK3β knockdown promotes pancreatic β cell and acinar cell regeneration in 90% pancreatectomized rat". Molecular ... "Loss of Fbw7 reprograms adult pancreatic ductal cells into α, δ, and β cells". Cell Stem Cell. 15 (2): 139-53. doi:10.1016/j. ... of acinar and duct cells in the histologically normal adult human pancreas. NGN3+ cells isolated from cultured exocrine tissue ... "Transient cytokine treatment induces acinar cell reprogramming and regenerates functional beta cell mass in diabetic mice". ...
"The distribution of phosphatidylinositol 4,5-bisphosphate in acinar cells of rat pancreas revealed with the freeze-fracture ... "eat-5 and unc-7 represent a multigene family in Caenorhabditis elegans involved in cell-cell coupling". J. Cell Biol. 134 (2): ... "Connexin43 modulates cell polarity and directional cell migration by regulating microtubule dynamics". PLoS ONE. 6 (10): e26379 ... When cells are compromised due to disease or injury and start to die messages are transmitted to neighboring cells connected to ...
Acinar cell carcinoma of the pancreas, also acinar cell carcinoma, is a rare malignant exocrine tumour of the pancreas. It ... Feb 2010). "[A case of acinar cell carcinoma of pancreas, manifested by subcutaneous nodule as initial clinical symptom]". ... Klimstra, DS.; Heffess, CS.; Oertel, JE.; Rosai, J. (Sep 1992). "Acinar cell carcinoma of the pancreas. A clinicopathologic ... Light microscopy of an acinar cell carcinoma biopsy typically shows granular appearance. Immunohistochemistry is usually ...
2005) Acinar Centre: Acinar and Centroacinar Cells. In: Functional Ultrastructure. Springer, Vienna. * DOI https://doi.org/ ... matrix of proteoglycans on zymogen granule membranes is involved in granule formation in rat pancreatic acinar cells. J Cell ...
... the powerful digestive enzymes that the cells make are initially produced in an inactive form, notes Pancreas.org. These inert ... The other cells of the exocrine pancreas are called duct cells. Acinar cells produce enzymes that turn vegetables, meat and ... Acinar cells are one of the two cells that make up the exocrine pancreas, a gland that secretes digestive enzymes into the ... To protect acinar cells from self-digestion, the powerful digestive enzymes that the cells make are initially produced in an ...
Cell Preparation and Extracellular Solutions.. Mouse pancreatic acinar cells were isolated by collagenase digestion as ... Isolated clusters of acinar cells were incubated in extracellular solution containing a cell-impermeant fluorescent tracer: ... Activation of trypsinogen in large endocytic vacuoles of pancreatic acinar cells. Mark W. Sherwood, Ian A. Prior, Svetlana G. ... Activation of trypsinogen in large endocytic vacuoles of pancreatic acinar cells. Mark W. Sherwood, Ian A. Prior, Svetlana G. ...
6 Studies found for: Acinar Cell Carcinoma , leucovorin. Also searched for Acinar Cell Adenocarcinomas. See Search Details ...
... Xianlin Zhao, Juan Li, Shifeng Zhu, Yiling Liu, Jianlei ... Cerulein-treated AR42J cells were used. After pretreatment with 479, 119.8, or 29.9 μg/L rhein, cells were cocultured with ... The Chinese herbal medicine Da-Cheng-Qi decoction can regulate a necrosis-apoptosis switch in injured pancreatic acinar cells. ... Rhein induces the necrosis-apoptosis switch in injured pancreatic acinar cells in a time- and dose-dependent manner. ...
Rat pancreatic acinar AR42J cells (CRL-1492, ATCC, Rockville, MD, USA) were seeded at a density of cells/well in flat-bottom 24 ... there were more necrotic cells than apoptotic cells. There were more apoptotic cells and fewer necrotic cells in T1, T2, and T3 ... Rhein Induces a Necrosis-Apoptosis Switch in Pancreatic Acinar Cells. Xianlin Zhao, Juan Li, Shifeng Zhu, Yiling Liu, Jianlei ... Y.-Y. Ren, H.-L. Gong, W.-F. Tang, M.-H. Wan, J.-L. Zhao, and X. Huang, "Dachengqi decoction induces pancreatic acinar cell ...
Activated Notch1 prevents differentiation of pancreatic acinar cells and attenuate endocrine development.. Hald J1, Hjorth JP, ... The endocrine cells that do form show abnormal expression of cell type-specific markers. Our observations show that sustained ... forms cyst-like structures with ductal phenotype containing a few endocrine cells but completely devoid of acinar cells. ... At e10.5 to e12.5, we observe a disorganized pancreatic epithelium with reduced numbers of endocrine cells, confirming a ...
... acinous cell. acinar cell, acinous cell synonyms, acinar cell, acinous cell pronunciation, acinar cell, acinous cell ... English dictionary definition of acinar cell, acinous cell. a small room as in a convent or prison; basic structural unit of ... secondary cell, storage cell - a cell that can be recharged. galvanic cell, primary cell, voltaic cell - an electric cell that ... cell. (redirected from acinar cell, acinous cell). Also found in: Thesaurus, Medical, Legal, Financial, Encyclopedia. cell. a ...
The left panel shows transmitted light image of isolated pancreatic acinar cells and clusters of pancreatic acinar cells. ... Bioluminescence was recorded from cells transfected with cLuc.. A. Images show bioluminescence of pancreatic acinar cells. The ... Dynamic changes in cytosolic and mitochondrial ATP levels in pancreatic acinar cells.. Voronina SG1, Barrow SL, Simpson AW, ... Dynamic changes in the ATP levels in the cytosol and mitochondria of pancreatic acinar cells: effects of calcium-releasing ...
... exhibit progressive apoptosis of pancreatic acinar cells with severe exocrine acinar cell loss by 8 wk of age, while the islets ... despite continued acinar cell loss. To our knowledge, this study describes the first characterized genetic animal model for ... of only the Serpini2 gene by bacterial artificial chromosome transgenic complementation corrected the acinar cell defect as ... complementation of the transgene insertion mutant demonstrates that Serpini2 deficiency directly results in the acinar cell ...
Normal acinar cells are the primary cells of the exocrine pancreas and are responsible for secreting various enzymes. ... Acinar cell neoplasms arise from the acinar cells of the pancreas. ... Acinar cell neoplasms arise from the acinar cells of the pancreas. Normal acinar cells are the primary cells of the exocrine ... Because the tumors arise from normal acinar cells, the tumor cells also may secrete pancreatic enzymes, most commonly lipase. ...
What is acinar cell? Meaning of acinar cell as a legal term. What does acinar cell mean in law? ... Definition of acinar cell in the Legal Dictionary - by Free online English dictionary and encyclopedia. ... cells, [beta]-cells, acinar cells, and ductal cells.. Targeting Cancer Stem Cells and Their Niche: Current Therapeutic ... Acinar cell legal definition of acinar cell https://legal-dictionary.thefreedictionary.com/acinar+cell ...
Acinar cell carcinoma (ACC) of the pancreas is extremely uncommon and its cytologic features have rarely been described. We ... Original cytologic diagnoses included "acinar cell carcinoma," "pancreatic endocrine tumor," "favor neuroendocrine tumor, ... The cytologic features included small to moderate-sized loose groups with numerous single cells, prominent acinar formation, ...
... ... Ca2+ Influx in Rat Pancreatic Acinar Cells." Biochemical and Biophysical Research Communications 202(3): 1697-1704. ,http://hdl ...
What is acinar cell? Meaning of acinar cell as a finance term. What does acinar cell mean in finance? ... Definition of acinar cell in the Financial Dictionary - by Free online English dictionary and encyclopedia. ... Related to acinar cell: Acinar cell carcinoma. cell. an independent team of operatives who work together in a CELLULAR ... Acinar cell financial definition of acinar cell https://financial-dictionary.thefreedictionary.com/acinar+cell ...
Acinar-Ductal Metaplasia and Biphenotypic Differentiation Are Observed in Acinar Cell-Derived mPanIN Lesions.. A recent ... Acinar-ductal metaplasia and biphenotypic differentiation are observed in acinar cell-derived mPanIN lesions. (A) Histological ... The concept of pancreatic acinar cells as a post-differentiated "dead end" cell type has undergone considerable reassessment in ... rigorous in vivo lineage tracing studies have established that transdifferentiation of acinar cells into nouveau β-cells or ...
Lacrimal gland acinar cell autoantigens in Sjögrens syndrome include both intracellular proteins and plasma membrane proteins ... We, therefore, suggest that a variety of physiologic stimuli may alter the spectra of autoantigens acinar cells secrete to the ... We review the vesicular traffic between acinar cell basal-lateral plasma membranes (blm) and endomembrane compartments, then ... B cell activating factor (BAFF), also called the B lymphocyte stimulator, has been known to show increased expression in ...
What is acinar cell, acinous cell? Meaning of acinar cell, acinous cell medical term. What does acinar cell, acinous cell mean? ... acinous cell in the Medical Dictionary? acinar cell, acinous cell explanation free. ... See also betz cells, gauchers cells, golgis cells, hela cells, hürthle cell, kupffers cells, merkel cell, mesangial cell, ... squamous cell See corneal epithelium.. W cell See C cell.. wing cell See corneal epithelium.. X cell See P cell.. Y cell See M ...
Transmission electron micrograph of glutaraldehyde fixed pancreatic acinar cell showing the characteristic features of nuclear ... Cell Type. pancreatic acinar cell Cellular Component. nucleus heterochromatin nucleolus ribosome rough endoplasmic reticulum ... Transmission electron micrograph of glutaraldehyde fixed pancreatic acinar cell showing the characteristic features of nuclear ... from: Don W. Fawcett M.D The Cell 2nd edition, W.B. Saunders Chapter 4 (Nucleus) Link. ASCB BioEDUCATE Program ...
Acinar cells also expressed the serous cell proteins LTF and AQP5.. Isolated acinar cells expressed mRNA encoding Muc5AC as ... and possibly goblet cells (Groneberg et al. 2003). Acinar cells (but not ciliated cells) also expressed the serous cell ... ciliated nasal epithelial cells, or salivary gland acinar cells as indicated. B, isolated mouse nasal acinar cells express ... Isolated mouse acinar cells express mRNA for serous acinar cell-specific markers A, Eberwine aRNA amplification reactions were ...
What is acinar cell carcinoma? Meaning of acinar cell carcinoma medical term. What does acinar cell carcinoma mean? ... Looking for online definition of acinar cell carcinoma in the Medical Dictionary? acinar cell carcinoma explanation free. ... acinar cell carcinoma. acinar cell carcinoma. A highly aggressive tumour that represents 1-2% of all pancreatic malignancies, ... Acinar cell carcinoma. A malignant tumor arising from the acinar cells of the pancreas. ...
PubMed - Acinar Cell Carcinoma (8 unread) * Activating transcription factor 3 promotes loss of the acinar cell phenotype in ... There were also no differences in acinar cell (P = .13) and duct cell replication (P = .099). Insulin-positive duct cells were ... Characterizing expression of transcription factors that affect acinar cell differentiation suggested that acinar cells lacking ... RATIONALE: Acinar cell carcinomas (ACCs) and mixed acinar-endocrine carcinomas (MAECs) of the pancreas are rare, accounting for ...
DNA SYNTHESIS, MITOSIS, AND DIFFERENTIATION IN PANCREATIC ACINAR CELLS IN VITRO. Norman K. Wessells ... Evidence suggests that cells or nuclei which have replicated DNA move inward before dividing. Some daughter cells apparently ... Cells located internally in explants, or in central acini in vivo, show this overt differentiation first. As the epithelia age ... The Journal of Cell Biology Mar 1964, 20 (3) 415-433; DOI: 10.1083/jcb.20.3.415 ...
PubMed - Acinar Cell Carcinoma (1 unread) * Metaplasia: tissue injury adaptation and a precursor to the dysplasia-cancer ... Rare Cancer News & Clinical Trials » PubMed - Acinar Cell Carcinoma » September 3, 2017. Share , ... The cell of origin for intestinal metaplasia in the oesophagus and stomach and for pancreatic acinar-ductal metaplasia has been ... Metaplasia is the replacement of one differentiated somatic cell type with another differentiated somatic cell type in the same ...
Keywords: acinar cell carcinoma, computed tomography, pancreatic ductal carcinoma, pancreas ... Pancreatic acinar cell carcinoma (ACC) is a rare tumor that is difficult to diagnose preoperatively. The aim of this study was ... A comparison study of pancreatic acinar cell carcinoma with ductal adenocarcinoma using computed tomography in Chinese patients ... Abstract: Pancreatic acinar cell carcinoma (ACC) is a rare tumor that is difficult to diagnose preoperatively. The aim of this ...
Serotonin regulates amylase secretion and acinar cell damage during murine pancreatitis Message subject: (Your Name) has ...
To confirm further the critical role of the PI3K/Akt pathway in pancreatic acinar cell proliferation, IGF-1-mediated cell ... Aging is associated with decreased pancreatic acinar cell regeneration and phosphatidylinositol 3-kinase/Akt activation ... Background & aims: The effects of aging on pancreatic acinar cell proliferation have not been clearly defined. ... its role in acinar cell proliferation is not known. The purpose of this study was to (1) delineate the effects of aging on ...
... in maintaining pancreatic acinar cell homeostasis, and provide evidence that defective lysosomal function, resulting in ... Lysosome associated membrane proteins maintain pancreatic acinar cell homeostasis: LAMP-2 deficient mice develop pancreatitis ... Conclusions: The results indicate a critical role for LAMPs, particularly LAMP-2, in maintaining pancreatic acinar cell ... LAMP-2 null mice spontaneously develop pancreatitis that begins with acinar cell vacuolization due to impaired autophagic flux ...
... youll find powerful enzymatic secretions which are the work of your pancreatic acinar cells. Pancreatic acinar cells are the ... and the proteins within the acinars own cells) from being digested by the enzymes it produces, acinar cells produce the ... Pancreatic acinar cells are the cells responsible for the synthesis, secretion, and storage of certain digestive enzymes. These ... Plant Cell Structures: The Cell Wall and Central Vacuole 5:26 * Eukaryotic and Prokaryotic Cells: Similarities and Differences ...
  • Acinar cell carcinoma of the pancreas, also acinar cell carcinoma, is a rare malignant exocrine tumour of the pancreas. (wikipedia.org)
  • To protect acinar cells from self-digestion, the powerful digestive enzymes that the cells make are initially produced in an inactive form, notes Pancreas.org. (reference.com)
  • Acinar cells are one of the two cells that make up the exocrine pancreas, a gland that secretes digestive enzymes into the intestines, notes Pancreas.org. (reference.com)
  • The other cells of the exocrine pancreas are called duct cells. (reference.com)
  • Further protection from self-digestion is provided by a trypsin inhibitor produced by acinar cells, which stops the enzyme from converting into an active form while still in the pancreas, notes the University of Washington. (reference.com)
  • By producing a fluid that flushes zymogens and any active enzymes into the small intestines, duct cells keep acinar cells and the rest of the pancreas safe from self-digestion. (reference.com)
  • After 10 days of a protein-free diet the acinar cells of the rat pancreas showed a coarsening of nuclear matrix, depletion of zymogen granules, some loss of ribosomes, and a widening of the spaces between ergastoplasmic membranes. (nih.gov)
  • Acinar cell neoplasms arise from the acinar cells of the pancreas. (knowcancer.com)
  • Normal acinar cells are the primary cells of the exocrine pancreas and are responsible for secreting various enzymes. (knowcancer.com)
  • Clinical characteristics and outcomes from an institutional series of acinar cell carcinoma of the pancreas and related tumors," Journal of Clinical Oncology, vol. (thefreedictionary.com)
  • Solid and cystic acinar cell tumor of the pancreas: a tumor in young women with favourable prognosis. (thefreedictionary.com)
  • Acinar cell carcinoma (ACC) of the pancreas is extremely uncommon and its cytologic features have rarely been described. (ovid.com)
  • Differential diagnosis of solid pseudo-papillary tumor of pancreas includes pancreatic neuroendocrine tumors, pancreatoblastoma, acinar cell carcinoma and pancreatic pseudocyst3,18. (thefreedictionary.com)
  • Histological examination of pancreas sectioned from L-arginine treated rats revealed tissue damage characterized by edema, inflammatory cell infiltrates, and acinar cell necrosis. (thefreedictionary.com)
  • In contrast to the uniformly penetrant mPanIN phenotype observed following developmental activation of Kras G12D in the Pdx1 -expressing progenitor cells, the Pdx1 -expressing population in the mature pancreas (predominantly islet β cells) appears to be relatively resistant to the effects of oncogenic Kras . (pnas.org)
  • The basis for the moniker "pancreatic ductal adenocarcinoma" (PDAC) is morphologic, and is attributable to misplaced attempts by the neoplastic cells to recapitulate normal tubulo-ductal structures in the pancreas. (pnas.org)
  • Because carcinogens can adversely impact multiple cell types in the pancreas, the lineage fidelity from cell-of-origin to eventual neoplasia is hard to preserve ( 9 ). (pnas.org)
  • A malignant tumor arising from the acinar cells of the pancreas. (thefreedictionary.com)
  • However, this PSA reactivity has also been described in acinar cell carcinoma of the pancreas [8]. (thefreedictionary.com)
  • The rare acinar cell carcinoma of the pancreas can be confirmed with stains for pancreatic enzymes such as trypsin, chymotrypsin, lipase, and elastase. (thefreedictionary.com)
  • In this lesson, we'll review the basics of the digestive process, go over the function of the pancreas, and explore the role of pancreatic acinar cells in breaking down food without breaking down our own tissues. (study.com)
  • False-colour transmission electron micrograph (TEM) featuring two elongated, acinar cells of the exocrine human pancreas. (sciencephoto.com)
  • Here, we report that PD2/Paf1 expression was restricted to acinar cells in the normal murine pancreas, but its expression increased in the ductal cells of KrasG12D/Pdx1Cre (KC) mouse model of pancreatic cancer with increasing age, showing highest expression in neoplastic ductal cells of 50 weeks old mice. (curehunter.com)
  • Although EtOH and its metabolites exert numerous effects on all cell types of the pancreas, the sensitization of pancreatic acinar cells to secretagogue signaling, premature intracellular zymogen activation, and deregulation of intracellular Ca 2+ homeostasis have been identified as critical early events in the pathogenesis of the disease ( 6 - 8 ). (pubmedcentralcanada.ca)
  • Studies on pancreas regeneration have focused mainly on the endocrine compartment, with the aim of gaining knowledge on β-cell regeneration. (biologists.org)
  • More recently, exocrine pancreas regeneration has been receiving more attention in the context of cancer and diabetes research because experimental models revealed a high capacity for cell fate plasticity ( Murtaugh and Keefe, 2015 ). (biologists.org)
  • During the formation of the pancreas, Nr5a2 is necessary for the expansion of the nascent pancreatic epithelium, for the subsequent formation of the multipotent progenitor cell (MPC) population that gives rise to pre-acinar cells and bipotent cells with ductal and islet endocrine potential, and for the formation and differentiation of acinar cells. (biologists.org)
  • The synthesis and secretion of these cells are closely regulated by the human body and alteration of these processes can lead to various pathologies of the exocrine pancreas, such as pancreatitis. (imim.es)
  • In recent years, interest in this type of cell has been marked by the fact that acinar cells can acquire a ductal phenotype and numerous pieces of evidence point to these as the possible origin of pancreatic ductal adenocarcinoma, the most common type of tumour of the pancreas and one of the most aggressive human tumours . (imim.es)
  • This fact has made it so that a new model has been established, in vitro using non-tumour cells, for the acinar differentiation study, a deregulated process in many diseases of the exocrine pancreas. (imim.es)
  • The size of the pancreas is determined by intrinsic factors, such as the number of progenitor cells, and by extrinsic signals that control the fate and proliferation of those progenitors. (biologists.org)
  • Although we have shown that the Wnt signaling component β-catenin is selectively required in mouse embryos for the generation of exocrine acinar cells, this protein has been ascribed various functions in the postnatal pancreas, including proliferation and regeneration of islet as well as acinar cells. (biologists.org)
  • Addressing these questions in the pancreas, a bifunctional organ comprising anatomically distinct endocrine and exocrine cell types, has implications for several devastating diseases, including diabetes, pancreatitis and pancreatic cancer. (biologists.org)
  • Aims Acinar cell carcinomas (ACCs) are rare tumours of the exocrine pancreas accounting for about 1-2% of all pancreatic neoplasms in adults. (bmj.com)
  • acinar cell carcinoma of the pancreas is a topic covered in the Taber's Medical Dictionary . (unboundmedicine.com)
  • Nursing Central , nursing.unboundmedicine.com/nursingcentral/view/Tabers-Dictionary/758421/0/acinar_cell_carcinoma_of_the_pancreas. (unboundmedicine.com)
  • Acinar cell carcinoma of the pancreas: an institutional series of resected patients and review of the current literature. (ucdenver.edu)
  • In pancreas, acinar-to-ductal metaplasia has been proposed as an initiating mechanism for pancreatic ductal adenocarcinoma (PDAC). (jci.org)
  • Ductal neoplasms of the human pancreas, as defined by KRAS2 gene mutations, do not appear to arise from acinar cells. (aacrjournals.org)
  • The acinar cell found in the pancreas, responsible for producing all the essential digestive enzymes in the small intestine, plays a vital role in the digestion of essential nutrients. (cancerworld.info)
  • Pancreas gland acinar cells. (gettyimages.be)
  • Isolated pancreatic acini and acinar cells can be prepared from rat, mouse, and guinea pig pancreas using a collagenase digestion and used for studies of secretion, ligand binding, substrate uptake, protein synthesis and second messenger generation. (pancreapedia.org)
  • Older animals may have excess fat in the pancreas which upon emulsification can be toxic to cells. (pancreapedia.org)
  • Context Acinar cell carcinoma is rare disease of exocrine pancreas with an indolent course and favorable tumor biology. (imedpub.com)
  • Histopathological examination with immunohistochemistry revealed acinar cell carcinoma of pancreas. (imedpub.com)
  • Conclusion Differential diagnosis of acinar cell carcinoma of pancreas should be considered in young patient with large tumor size. (imedpub.com)
  • Acinar, centroacinar, and endocrine cells of the adult rat pancreas each exhibit distinctive cell‐surface glycoconjugate patterns as detected by binding of a battery of lectin‐ferritin conjugates. (elsevier.com)
  • Studies on the cell‐surface properties of a rat acinar cell tumor 20 indicate that the neoplastic acinar cells are likely to be arrested at a developmental stage equivalent to acinar cells of the day 19 embryonic pancreas and are characterized by absence of detectable basal lamina. (elsevier.com)
  • The presence of substance P and its receptor, neurokinin-1 receptor (NK1R) has been shown in the pancreas and the pancreatic acinar cells. (aspetjournals.org)
  • Pancreatic acinar cells were obtained from pancreas of male Swiss mice. (aspetjournals.org)
  • Whether the diabetes is transient (temporary) or permanent depends on the severity of the damage to the endocrine pancreas beta cells. (wikipedia.org)
  • Acinar cell carcinoma (ACC) of the pancreas is a rare neoplasm, accounting only 1-2% of pancreatic cancers, and is characterized by exhibiting exocrine differentiation and lacking significant ductal or endocrine differentiation. (oatext.com)
  • Preferential accumulation of acridine orange was resolved in large intracellular vacuoles generated in pancreatic acinar cells, suggesting their acidic nature ( 33 ). (pnas.org)
  • All of them accepted that the secretory acinar cells (SAC) have the major role in the pathogenesis of pancreatitis, which contains abnormal active zymogens [9]. (thefreedictionary.com)
  • In a variety of secretory cells, this fusion pore has now been shown to subsequently close. (nih.gov)
  • Accordingly, agonist-induced serous acinar cell shrinkage and swelling are caused by activation of solute efflux and influx pathways, respectively, and cell volume reflects the secretory state of these cells. (wiley.com)
  • To further understand the physiopathological steps of those diseases, our aim is to establish a lacrimal gland acinar cell culture line and to promote changes in culture conditions to evaluate the impact on secretory parameters. (arvojournals.org)
  • We therefore conclude that these polarized secretory cells contain two pools of Orai1: an apical pool that interacts with IP 3 Rs and a basolateral pool that interacts with STIM1 following the Ca 2+ store depletion. (biochemj.org)
  • PACs (pancreatic acinar cells) are structurally and functionally polarized with secretory granules located in the apical region, whereas the basal and lateral parts contain well-developed rough ER (endoplasmic reticulum). (biochemj.org)
  • Influence of culture conditions on the androgen control of secretory component production by acinar cells from the rat lacrimal gland. (arvojournals.org)
  • Research has shown that androgens regulate the production of secretory component (SC), the IgA antibody receptor, by lacrimal gland acinar cells in vivo. (arvojournals.org)
  • The pancreatic acinar cell is perhaps the best studied such cell, and the classical model for stimulus-secretion coupling and secretory protein biosynthesis and export. (elsevier.com)
  • The Ca 2+ dependence of surface membrane retrieval (i.e., the process by which the excess surface membrane resulting from exocytosis is recycled to the cytoplasm of secretory cells) has been investigated in rat parotid tissue lobules first incubated for 40 min in the presence of a secretagogue drug (the β-adrenergic agonist isoprenaline) and then in the presence of the β-blocker, 1-propranolol, up to 4 h. (elsevier.com)
  • However, little is known about the involvement of Rab protein(s) in secretory granule exocytosis in exocrine cells or the molecular mechanism underlying this process. (elsevier.com)
  • We show that Rab27B, a closely related isoform of Rab27A that regulates lysosome-related granule exocytosis in cytotoxic T lymphocytes, is abundantly expressed on amylase-containing secretory granules in rat parotid gland acinar cells. (elsevier.com)
  • Our results indicate that the Rab27B-Slac2-c complex is an important constituent of secretory granule exocytosis in parotid acinar cells. (elsevier.com)
  • The combination with a saponification method also allowed the occurrence of C4 acetylated sialic acids linked to beta-galactose to be discovered, at the electron microscope level, on acinar cell secretory products. (unicam.it)
  • abstract = "Transforming growth factor-β (TGF-β) inhibits pancreatic acinar cell growth. (elsevier.com)
  • abstract = "Background: The pancreatic exocrine cell line AR42J-B13 can be reprogrammed to hepatocytes following treatment with dexamethasone. (bath.ac.uk)
  • Ca 2+ signalling in exocrine acinar cells has been shown to be both polarized and pulsatile in all cell types tested, except acutely isolated mouse lacrimal acinar cells. (portlandpress.com)
  • Activated Notch1 prevents differentiation of pancreatic acinar cells and attenuate endocrine development. (nih.gov)
  • Mice carrying loss-of-function mutations in certain Notch pathway genes display increased and accelerated pancreatic endocrine development, leading to depletion of precursor cells followed by pancreatic hypoplasia. (nih.gov)
  • At e10.5 to e12.5, we observe a disorganized pancreatic epithelium with reduced numbers of endocrine cells, confirming a repressive activity of Notch1 upon the early differentiation program. (nih.gov)
  • Subsequent branching morphogenesis is impaired and the pancreatic epithelium forms cyst-like structures with ductal phenotype containing a few endocrine cells but completely devoid of acinar cells. (nih.gov)
  • The endocrine cells that do form show abnormal expression of cell type-specific markers. (nih.gov)
  • Our observations show that sustained Notch1 signaling not only significantly represses endocrine development, but also fully prevents pancreatic exocrine development, suggesting that a possible role of Notch1 is to maintain the undifferentiated state of common pancreatic precursor cells. (nih.gov)
  • Original cytologic diagnoses included "acinar cell carcinoma," "pancreatic endocrine tumor," "favor neuroendocrine tumor, lowgrade" and "non-diagnostic specimen. (ovid.com)
  • Other studies, based on chemical carcinogenesis models in Syrian hamsters or in rats, have postulated nonductal origins of PDAC, namely from the endocrine and acinar compartments, respectively ( 7 , 8 ). (pnas.org)
  • The radiologic differential diagnosis for these tumors is broad and includes a number of benign and malignant processes, such as mucinous cystic neoplasm, serous microcystic adenoma, ductal adenocarcinoma, pancreatic endocrine neoplasm, acinar cell carcinoma , solid pseudopapillary tumor, and autoimmune pancreatitis. (thefreedictionary.com)
  • Sensitivities for specific diagnostic entities were as follows: 79% for ductal adenocarcinoma, 100% for acinar cell carcinoma , 75% for pancreatic endocrine tumor, 70% for intraductal papillary mucinous neoplasms (IPMN), and 42% for mucinous cystic neoplasms (MCN). (thefreedictionary.com)
  • Many of the major types of pancreatic neoplasms (ductal adenocarcinoma, pancreatic endocrine neoplasm, acinar cell carcinoma , pancreatoblastoma) typically form solid masses (Figure 4, A and B). (thefreedictionary.com)
  • The mature organ is composed of an exocrine compartment with acinar and duct cells that produce and transport digestive enzymes into the gut, and an endocrine compartment from which metabolism-regulating peptide hormones including insulin are secreted into the blood stream. (biologists.org)
  • 5) separation of the endocrine and ductal programs, morphogenetic resolution of a precursor ductal plexus and completion of cell type-specific differentiation. (biologists.org)
  • This process creates three interrelated tissues: the islets of Langerhans (endocrine cells that produce polypeptide hormones, including insulin and glucagon), acini (exocrine cells that produce and secrete hydrolytic digestive enzymes) and ducts (exocrine cells that produce a bicarbonate flush and channel the acinar secretions to the intestine). (biologists.org)
  • Yet, the molecular specifics of the regulatory transitions are incompletely understood - especially the formation of the multipotent cell population and the resolution of acinar precursor cells distinct from ductal and endocrine precursors. (biologists.org)
  • This study was designed to establish an optimal culture system to permit analysis of this endocrine-acinar cell interrelationship in vitro. (arvojournals.org)
  • A diagnosis of pancreatic acinar cell carcinoma was established in both horses. (nottingham.ac.uk)
  • Acinar Bcl-2 expression exceeded islet expression in chronic pancreatitis and controls while Bax was strongly expressed by a subset of islet cells and weakly by centroacinar cells. (soton.ac.uk)
  • however, selective expression of endogenous mutant KRAS in acinar/centroacinar cells during early embryonic development produces a full range of mouse PanINs (mPanIN) and invasive pancreatic ductal adenocarcinoma ( 16 - 18 ). (aacrjournals.org)
  • We therefore investigated the site of intracellular trypsinogen activation by using an established cellular model of acute pancreatitis: supramaximal stimulation of pancreatic acinar cells with cholecystokinin. (pnas.org)
  • The intracellular distribution of trypsin activation sites can be probed by antibodies for trypsinogen-activation peptide (TAP) ( 8 , 9 , 16 ) and in living cells by rhodamine 110 bis-(CBZ- l -isoleucyl- l -prolyl- l -arginine amide) dihydrochloride (BZiPAR), a substrate for trypsin that becomes fluorescent after cleavage by the protease ( 10 , 12 , 17 ). (pnas.org)
  • Previous studies of pancreatic acinar cells characterized the effects of Ca(2+)-releasing secretagogues and substances, inducing acute pancreatitis on mitochondrial Ca(2+), transmembrane potential, and NAD(P)H, but dynamic measurements of the crucial intracellular adenosine triphosphate (ATP) levels have not been reported. (nih.gov)
  • The pathophysiology of acute pancreatitis is not obvious, although intracellular activation of the digestive enzymes in acinar cells is accepted as the starting point. (thefreedictionary.com)
  • Lacrimal gland acinar cell autoantigens in Sjögren's syndrome include both intracellular proteins and plasma membrane proteins, to which the immune system normally must be tolerant. (ebscohost.com)
  • Lacrimal cells are also unusual in that they display a very low sensitivity to Ins(1,4,5) P 3 (Ins P 3 ) that may be enhanced by placing the cells in primary culture for 12-72h or by intracellular infusion of a low concentration of Ins(1,3,4,5) P 4 (Ins P 4 ). (portlandpress.com)
  • However, once the pancreatic cells died, necrotic breakdown occurred very rapidly, perhaps due to intracellular release of lysosomal and zymogen granule hydrolases. (springer.com)
  • Furthermore, we show that either suppression of Raf-1 kinase inhibitory protein expression using short hairpin RNA or gene ablation prevented the sensitizing effects of ethanol on cholecystokinin- and carbachol-stimulated Ca 2+ signaling and intracellular chymotrypsin activation in pancreatic acinar cells, suggesting that the modulation of Raf-1 inhibitory protein expression may have future therapeutic utility in the prevention or treatment of alcohol-associated pancreatitis. (pubmedcentralcanada.ca)
  • In resting acinar cells, the rab3-like protein was detected primarily on the cytoplasmic face of ZGs, with little labeling of the Golgi complex and no significant labeling of the apical plasmalemma or any other intracellular membranes. (rupress.org)
  • Incubation of permeabilized acinar cells in a calcium-free intracellular milieu, as well as application of thapsigargin, resulted in complete inhibition of the uptake of Ca 2+ with the involvement of SERCA. (springer.com)
  • The effects of extracellular ATP on ion fluxes and the intracellular free Ca2+ concentration ([Ca2+]i) were examined using a suspension of rat parotid acinar cells and were contrasted with the effects of the muscarinic agonist carbachol. (rupress.org)
  • ATP and carbachol also stimulated the rapid entry of Na+ into the parotid cell, and elevated the intracellular Na+ content to 4.4 and 2.6 times the normal level, respectively. (rupress.org)
  • The past year has witnessed a marked increase in the complexity of intracellular processes involved in the function of a polarized epithelial cell. (elsevier.com)
  • The dye passively diffuses into cells, where its acetate groups are cleaved by intracellular esterases and its thiol-reactive chloromethyl group reacts with intracellular glutathione and other thiols. (pancreapedia.org)
  • Maintenance of acinar structure requires preservation of intracellular junctional complexes and further breakdown to the single cell level requires treatment with protease and/or calcium chelation. (pancreapedia.org)
  • Isolated pancreatic acini and isolated acinar cells have become the preparation of choice for many studies of acinar cell function including digestive enzyme synthesis and secretion, signal transduction events including measurement of intracellular Ca 2+ , and ligand binding studies. (pancreapedia.org)
  • 5 cm CVRL (185th day) buffalo foetus showing myoepithelial cell (MEC) at the base of the acinar cell of parotid gland. (thefreedictionary.com)
  • Acinar cell carcinomas are usually well differentiated and account for about 13% of the cancers arising in the parotid gland. (ucdenver.edu)
  • Involvement of apoptosis and proliferation of acinar cells in atrophy of rat parotid glands induced by liquid diet. (biomedsearch.com)
  • To clarify whether apoptosis and proliferation of acinar cells participate in atrophy of rat parotid glands induced by liquid diet, rats were fed a liquid diet and compared to pellet-fed controls. (biomedsearch.com)
  • Enhanced proliferation of acinar and progenitor cells by prophylactic pilocarpine treatment underlies the observed amelioration of radiation injury to parotid glands. (semanticscholar.org)
  • The Chinese herbal medicine Da-Cheng-Qi decoction can regulate a necrosis-apoptosis switch in injured pancreatic acinar cells. (hindawi.com)
  • This study investigated the effects of rhein, a component of this medicine, on a necrosis-apoptosis switch in pancreatic rat AR42J cells. (hindawi.com)
  • Rhein induces the necrosis-apoptosis switch in injured pancreatic acinar cells in a time- and dose-dependent manner. (hindawi.com)
  • The severity of AP is influenced by the balance between two forms of cell death, namely, apoptosis and necrosis. (hindawi.com)
  • Therefore, apoptosis induction in injured pancreatic acinar cells might suppress the inflammatory response and alleviate the severity of AP and could thereby be an effective treatment strategy [ 1 - 3 ]. (hindawi.com)
  • Under certain conditions, a necrosis-apoptosis switch serves to modulate how cells die [ 4 ]. (hindawi.com)
  • Consequently, the necrosis-apoptosis switch has been studied in pancreatic acinar cells. (hindawi.com)
  • We previously showed that DCQD can ameliorate pancreatic inflammation and pathological damage by inducing a necrosis-apoptosis switch in AR42J cells and a rat model of AP [ 7 ]. (hindawi.com)
  • Other studies similarly reported that treatment with various components of DCQD induces apoptosis and reduces necrosis of acinar cells in AP [ 8 - 10 ]. (hindawi.com)
  • Rhein can promote apoptosis of cancer cells through the mitochondria-dependent pathway [ 12 , 13 ]. (hindawi.com)
  • In this study, we investigated the proapoptotic and antinecrotic effects of rhein on AR42J cells and analyzed the molecular mechanism underlying how rhein induces a necrosis-apoptosis switch by examining mitochondria-dependent apoptosis signaling pathways. (hindawi.com)
  • Calcium signalling and pancreatic cell death: apoptosis or necrosis? (findaphd.com)
  • These findings suggest that increased acinar cell apoptosis and reduced acinar cell proliferation occur in atrophic parotid glands of rats fed a liquid diet. (biomedsearch.com)
  • We introduced and validated two novel transgenic approaches for genetically encoded conditional cell ablation in the zebrafish, either by caspase-8-induced apoptosis or by rendering cells sensitive to diphtheria toxin. (biologists.org)
  • CONCLUSION: Apoptosis plays a significant role in acinar loss in chronic pancreatitis. (soton.ac.uk)
  • Fas ligand expression may be restricted to islet cell membranes through TIMP-1 expression and inhibit islet damage by promoting apoptosis of cytotoxic T lymphocytes. (soton.ac.uk)
  • Under identical conditions, mice either deficient in MMP-7 or carrying an inactive FasL gene are severely inhibited in development of progressive metaplasia and acinar cell apoptosis. (jci.org)
  • The main cause of Xerostomia is salivary epithelial cells apoptosis. (grantome.com)
  • We believe that our findings here will greatly contribute to public contribute to public as understanding the mechanism regulating nuclear import of PKC5 will give insight into mechanism by which PKC6 regulates apoptosis in the salivary epithelial cells which is crucial for the development of therapeutic strategies to protect against salivary gland apoptosis. (grantome.com)
  • Because Bcl-2 family members protein play a significant part in apoptosis, we assessed the apoptosis induction by Bcl-2 family members inhibitor BH3I-2 in three group of independent tests with 20C80 cells each. (healthweblognews.info)
  • In this study, the authors analysed whether 5-HT regulates zymogen secretion in pancreatic acinar cells and the development of pancreatic inflammation, a potentially lethal disease whose pathophysiology is not completely understood. (bmj.com)
  • Conclusions These results identify a 5-HT-mediated regulation of zymogen secretion in pancreatic acinar cells. (bmj.com)
  • Physiological roles of low molecular weight 20KG GTP-binding protein : Effects on secretion in pancreatic acinar cells. (nii.ac.jp)
  • Present study aims to investigate whether neutrophils primed by 4beta-phorbol 12beta-myristate 13alpha-acetate (PMA) affect the productions of H(2)O(2) and lipid peroxide (LPO), NF-kappaB activation and cytokine production in pancreatic acinar cells, and whether these alterations were inhibited by N-acetylcysteine (NAC) and superoxide dismutase (SOD). (yonsei.ac.kr)
  • Here, we examine the behavior of the fusion pore during zymogen granule exocytosis in pancreatic acinar cells. (nih.gov)
  • Together, our data do not support the classical view in acinar cells that exocytosis ends with granule collapse. (nih.gov)
  • Regulated secretion from pancreatic acinar cells occurs by exocytosis of zymogen granules (ZG) at the apical plasmalemma. (rupress.org)
  • dependent exocytosis or increases in membrane capacitance in these cells. (nii.ac.jp)
  • Publications] Maruyama,Y.: 'Control of exocytosis in single cells' News in physiol.Sci.4. (nii.ac.jp)
  • Eating stimulates neural and hormonal pathways that mediate acinar cell zymogen granule exocytosis into the pancreatic duct. (pancreapedia.org)
  • Metaplasia is the replacement of one differentiated somatic cell type with another differentiated somatic cell type in the same tissue. (rare-cancer.org)
  • The cell of origin for intestinal metaplasia in the oesophagus and stomach and for pancreatic acinar-ductal metaplasia has been posited through genetic mouse models and lineage tracing but has not been identified in other types of metaplasia, such as squamous metaplasia. (rare-cancer.org)
  • PD2/Paf1 depletion in pancreatic acinar cells promotes acinar-to-ductal metaplasia. (curehunter.com)
  • PD2/Paf1 was specifically expressed in amylase and CK19 double positive metaplastic ducts, representing intermediate structures during pancreatic acinar-to-ductal metaplasia (ADM). (curehunter.com)
  • In a mouse model of pancreatic acinar-to-ductal metaplasia, MMP-7 progressively accumulates during the metaplastic transition, resulting in a concomitant increase in solubilization of Fas ligand (FasL). (jci.org)
  • To integrate findings in mice and men, we examined human acinar cells, acinar-ductal metaplasia (ADM) lesions, and PanINs for KRAS2 gene mutations. (aacrjournals.org)
  • In addition, acinar-ductal metaplasia (ADM) is a prominent component of many of these genetically engineered mouse models ( 17 - 19 ), and in some models, ADM appears to precede the appearance of mPanIN lesions ( 17 ). (aacrjournals.org)
  • Stimulation of isolated acinar cells with carbachol (CCh), histamine or ATP was associated with marked decreases in cell volume (20 ± 2% within 62 ± 5 s) that were tightly correlated with increases in cytoplasmic Ca 2+ concentration ([Ca 2+ ] i ) as revealed by simultaneous DIC and fluorescent indicator dye microscopy. (wiley.com)
  • These results provide insights into the molecular mechanisms of salt and water secretion by lung submucosal glands, and they suggest that while murine submucosal gland fluid secretion in response to cholinergic stimulation can originate from CFTR-expressing serous acinar cells, it is not dependent upon CFTR function. (wiley.com)
  • Muscarinic acetylcholine receptors (mAChRs) expressed by pancreatic acinar cells play an important role in mediating acetylcholine-dependent stimulation of digestive enzyme secretion. (aspetjournals.org)
  • Strikingly, carbachol-induced stimulation of amylase secretion was significantly impaired in acinar preparations from both M 1 and M 3 receptor single KO mice and abolished in acinar preparations from M 1 /M 3 receptor double KO mice. (aspetjournals.org)
  • Nevalainen, T. J.: Effects of pilocarpine stimulation on rat pancreatic acinar cells. (springer.com)
  • When appropriate microenvironmental cues were provided in a controlled manner in 3D, such as stimulation with β-adrenergic and cholinergic agonists, hS/PCs differentiated into an acinar-like lineage, needed for saliva production. (rice.edu)
  • With this stimulation, some cells showed increases in membrane capacitance but some did not. (nii.ac.jp)
  • The dynamics of the luminal surface membrane was monitored by measuring, in ultrathin sections, the length of the luminal profile of all examined acinar cells abutting to a lumen before and immediately at the end of the stimulation, as well as at various times thereafter. (elsevier.com)
  • After the stimulation was blocked, the luminal profile decreased to reach values even lower than those observed in unstimulated cells. (elsevier.com)
  • Koike, H & Meldolesi, J 1981, ' Post-stimulation retrieval of luminal surface membrane in parotid acinar cells is calcium-dependent ', Experimental Cell Research , vol. 134, no. 2, pp. 377-388. (elsevier.com)
  • Physiological stimulation of pancreatic acinar cells by cholecystokinin and acetylcholine activate a spatial-temporal pattern of cytosolic [Ca+2] changes that are controlled with a coordinated response of inositol 1,4,5-trisphosphate receptors (IP3Rs), ryanodine receptors (RyRs) and calcium-induced calcium release (CICR). (healthweblognews.info)
  • Stimulation of pancreatic acinar cells with SP was found to up-regulate the production of inflammatory mediators, specifically chemokines such as monocyte chemotactic protein-1 and macrophage inflammatory protein 1α ( Ramnath and Bhatia, 2006 ). (aspetjournals.org)
  • It may be concluded that the cytoplasm of acinar cells was filled with mitochondria, rough endoplasmic reticulum and Golgi complex in mid and late foetal age groups. (thefreedictionary.com)
  • We have proposed previously that this atypical behaviour stemmed from vesiculation of the endoplasmic reticulum (ER) incurred during isolation of the cells and, furthermore, that time in culture or Ins P 4 increased sensitivity to Ins P 3 by increasing ER integrity [Smith, Harmer, Letcher and Irvine (2000) Biochem. (portlandpress.com)
  • These changes ranged from dilated endoplasmic reticulum and swollen mitochondria (reversible changes) to mitochondrial flocculent densities and later stages (evidence of cell death). (springer.com)
  • W. Paschen and A. Frandsen, "Endoplasmic reticulum dysfunction a common denominator for cell injury in acute and degenerative diseases of the brain? (springer.com)
  • Basal and physiological Ca 2+ leak from the endoplasmic reticulum of pancreatic acinar cells. (springer.com)
  • As there is no good record of the patients with acinar cell carcinoma existing, all information is collected from small case series and case reports. (cancerworld.info)
  • Five patients had a germline PRKAR1A mutation, including two patients with acinar cell carcinoma, for whom mutations were found in a hemizygous state in the tumor, suggesting loss of heterozygosity. (cdc.gov)
  • Histomorphologically, the tumour resembles the cells of the pancreatic acini and, typically, have moderate granular cytoplasm that stain with both PAS and PASD. (wikipedia.org)
  • acinar cell , acinic cell , acinous cell any of the cells lining an acinus, especially the zymogen-secreting cells of the pancreatic acini. (thefreedictionary.com)
  • Cells located internally in explants, or in central acini in vivo , show this overt differentiation first. (rupress.org)
  • Methods 5-HT regulation of zymogen secretion was analysed in pancreatic acini isolated from wild-type or tryptophan hydoxylase-1 knock-out (TPH1 −/− ) mice, which lack peripheral 5-HT, and in amylase-secreting pancreatic cell lines. (bmj.com)
  • Microscopically, neoplastic cells were organized in acini and contained abundant (case 1) or sparse (horse 2) intracytoplasmic zymogen granules. (nottingham.ac.uk)
  • Isolated pancreatic acini and acinar cells can be prepared from rat, mouse, and guinea pig. (pancreapedia.org)
  • This review describes the method used in our laboratory to isolate acini, variations in the method as used by others and ways of assessing the quality of the acinar preparation based on morphology and amylase secretion. (pancreapedia.org)
  • Isolated or dispersed exocrine pancreatic cells were first described by Amsterdam and Jamieson in 1972 and have been used by multiple investigators since then (1,2,11,33) followed by the description of isolated acini in 1978 which are now the standard preparation (6,24,30,34). (pancreapedia.org)
  • Acini are purified away from ducts, islets and blood vessels based on size and density although some contamination from other cell types remains. (pancreapedia.org)
  • Isolated acini which are usually composed of 8-20 cells retain their junctional complexes (tight and adherens junctions) and three dimensional architecture for short term studies of 2-6 hours. (pancreapedia.org)
  • An acinar prep can be produced from a single mouse although when more acini are required several pancreases can be digested simultaneously in the same flask. (pancreapedia.org)
  • de Dios, "Kinetic study of TNF-[alpha]lpha production and its regulatory mechanisms in acinar cells during acute pancreatitis induced by bilepancreatic duct obstruction," The Journal of Pathology, vol. (thefreedictionary.com)
  • Activating transcription factor 3 promotes loss of the acinar cell phenotype in response to cerulein-induced pancreatitis in mice. (rare-cancer.org)
  • Although down-regulation of genes that promote the mature acinar cell fate is required to reduce injury associated with pancreatitis, the factors that promote this repression are unknown. (rare-cancer.org)
  • We have shown that insulin, which is protective in animal models and clinical studies of human pancreatitis, protects pancreatic acinar cells from cytosolic Ca2+ overload, inhibition of the plasma membrane Ca2+ pump (PMCA), ATP depletion and necrotic cell death induced pancreatitis-causing agents. (findaphd.com)
  • 2009). Fatty acids, alcohol and fatty acid ethyl esters: toxic Ca2+ signal generation and pancreatitis Cell Calcium. (findaphd.com)
  • These findings provide novel insights into the normal physiology of pancreatic acinar cells and into the pathophysiology of pancreatitis, with potential therapeutic implications. (bmj.com)
  • The inducers of acute pancreatitis trigger a prolonged increase in the cytosolic Ca 2+ concentration ([Ca 2+ ] c ), which is responsible for the damage to and eventual death of pancreatic acinar cells. (biochemj.org)
  • Inducers of acute pancreatitis (bile acids, non-oxidative ethanol metabolites and supramaximal doses of secretagogues) trigger characteristic Ca 2+ responses in pancreatic acinar cells [ 8 - 14 ]. (biochemj.org)
  • Excessive alcohol consumption is associated with most cases of chronic pancreatitis, a progressive necrotizing inflammatory disease that can result in pancreatic insufficiency due to acinar atrophy and fibrosis and an increased risk of pancreatic cancer. (pubmedcentralcanada.ca)
  • Additional evidence that these transporters are expressed in mature acinar cells was gained using acinar cell culture and acute pancreatitis models. (garvan.org.au)
  • In the acute phase of pancreatic injury, when acinar cell loss occurs, and in an acinar cell culture model, which mimics changes occurring during pancreatitis, SNAT3 and SNAT5 are strongly down-regulated. (garvan.org.au)
  • In humans and mammalian model systems, the partial loss of exocrine tissue, such as after acute pancreatitis or partial pancreatectomy induces rapid recovery via expansion of surviving acinar cells. (biologists.org)
  • We suggest that loss of Wnt-β-catenin activity is unlikely to drive islet dysfunction, as occurs in type 2 diabetes, but that β-catenin is likely to promote human acinar cell proliferation following injury, and might therefore contribute to the resolution of acute or chronic pancreatitis. (biologists.org)
  • AIMS: To test the hypothesis that acinar loss and islet preservation in chronic pancreatitis occurs due to differential epithelial kinetics and investigate the role of inflammatory cells and cell cycle associated molecules. (soton.ac.uk)
  • RESULTS: The acinar cell apoptotic index (AI) and proliferation index were higher in chronic pancreatitis than controls. (soton.ac.uk)
  • Islet Fas and Fas ligand expression exceeded acinar expression in chronic pancreatitis and controls. (soton.ac.uk)
  • Acinar Rb expression was higher in chronic pancreatitis than in controls. (soton.ac.uk)
  • Increased acinar Rb expression in chronic pancreatitis may differentially promote acinar loss. (soton.ac.uk)
  • Adipose Stem Cell Therapy Mitigates Chronic Pancreatitis via Differentiation into Acinar-like Cells in Mice. (semanticscholar.org)
  • A plasmalemmal site of insulation of this important receptor on the pancreatic acinar cell could be particularly effective to protect the cell from processes which might initiate pancreatitis, while providing for the rapid resensitization of this receptor to ensure appropriate pancreatic secretion to aid in nutrient assimilation for the organism. (rupress.org)
  • Isolated cells were treated with caerulein to mimic secretagogue pancreatitis. (aspetjournals.org)
  • One study indicated that 57 percent of dogs, who were followed for six months after an acute pancreatitis attack, either continued to exhibit inflammation of the organ or had decreased acinar cell function, even though they had no pancreatitis symptoms. (wikipedia.org)
  • Because the tumors arise from normal acinar cells, the tumor cells also may secrete pancreatic enzymes, most commonly lipase. (knowcancer.com)
  • Smoking is the other important pancreatic cancer risk factor, but because acinar cell tumors are so rare, this association cannot be firmly established. (knowcancer.com)
  • Because acinar cell tumors are so uncommon, there is little known about the long-term prognosis. (knowcancer.com)
  • Acinar cell tumors are very rare and therefore cannot reasonably be studied in randomized, controlled clinical trials that compare treatments. (knowcancer.com)
  • The drugs that appear to be most active against acinar cell tumors include fluorouracil (with leucovorin) and irinotecan. (knowcancer.com)
  • Askanazy cells large eosinophilic cells found in the thyroid gland in autoimmune thyroiditis and Hürthle cell tumors. (thefreedictionary.com)
  • Acinar cell tumors are highly malignant with a predisposition to recur [3]. (oatext.com)
  • These findings indicate that a neoplastic human salivary intercalated duct cell is capable of at least bidirectional differentiation. (aacrjournals.org)
  • Results Non-neoplastic acinar cells were stained diffusely, but epithelial cells of the pancreatic duct and the islets of Langerhans were not stained. (bmj.com)
  • In case 2, a low percentage of neoplastic cells expressed glucagon and synaptophysin. (nottingham.ac.uk)
  • Kras G12D (KC) mouse model of pancreatic cancer with increasing age, showing highest expression in neoplastic ductal cells of 50 weeks old mice. (oncotarget.com)
  • Isolated AMD lesions are genetically distinct from those associated with PanINs, and the latter may represent retrograde extension of the neoplastic PanIN cells or less likely are precursors to PanIN. (aacrjournals.org)
  • We hypothesize that pancreatic cancers may arise from the equivalent of the undifferentiated embryonic stem cell(s) and that the morphologic features of the tumor depend on the extent of cell differentiation, including expression of cell‐surface glycoproteins and extracellular matrix, prior to neoplastic proliferation. (elsevier.com)
  • Contrary to conventional immunohistochemical diagnostic criteria for ACC, the neoplastic cells were immunoreactive with CD10 and negative for keratin. (oatext.com)
  • Tissue edema, leukocyte infiltration, acinar cell necrosis, and hemorrhage were the pathological changes observed in this model. (thefreedictionary.com)
  • The acinar cell derivation of the mPanINs was established through lineage tracing in reporter mice, and by microdissection of lesional tissue demonstrating Cre -mediated recombination events. (pnas.org)
  • 1. any of the protoplasmic masses making up organized tissue, consisting of a nucleus surrounded by cytoplasm enclosed in a cell or plasma membrane. (thefreedictionary.com)
  • Arias-Stella cells columnar cells in the endometrial epithelium which have a hyperchromatic enlarged nucleus and which appear to be associated with chorionic tissue in an intrauterine or extrauterine site. (thefreedictionary.com)
  • In older explants, thymidine incorporation is largely restricted to the periphery of the tissue as zymogen appears in the internal cells. (rupress.org)
  • Here, we report an expandable population of primary salivary human stem/progenitor cells (hS/PCs) that can be reproducibly and scalably isolated and propagated from tissue biopsies. (rice.edu)
  • 95% of acinar tissue in larval and adult zebrafish without causing any signs of unspecific side effects. (biologists.org)
  • We find that zebrafish larvae are able to recover from a virtually complete acinar tissue ablation within 2 weeks. (biologists.org)
  • In conclusion, we show a conserved requirement for Wnt signaling in exocrine tissue expansion and reveal a potential novel progenitor or stem cell population as a source for exocrine neogenesis after complete loss of acinar cells. (biologists.org)
  • This study investigated the transdifferentiation of stem cells from human amnion tissue into functional acinar cells (ACs) using a co-culture system. (semanticscholar.org)
  • Human amniotic epithelial cells (hAECs) were isolated from amnion tissue by mechanical mincing and enzymatic digestion. (semanticscholar.org)
  • Concise Review: Salivary Gland Regeneration: Therapeutic Approaches from Stem Cells to Tissue Organoids. (semanticscholar.org)
  • Development of Causative Treatment Strategies for Lacrimal Gland Insufficiency by Tissue Engineering and Cell Therapy. (semanticscholar.org)
  • a core biopsy of the liver showing liver tissue adjacent to the acinar cell carcinoma, haematoxylin/eosin staining, 10-fold magnification. (biomedcentral.com)
  • While these kits are useful for some tissue or cell types, they are not especially cost-effective and have proven to be insufficient for use with adult pancreatic tissue or isolated acinar cells. (pancreapedia.org)
  • After examining the efficiency and yield provided by a variety of ready-made ChIP kits, our laboratory has developed our own ChIP protocols for use with isolated acinar cells or whole pancreatic tissue. (pancreapedia.org)
  • The isolation of acinar cells also removes excess connective tissue that may interfere with efficient processing. (pancreapedia.org)
  • The following are protocols developed by our lab for ChIP analysis from either isolated acinar cells or from whole tissue. (pancreapedia.org)
  • While the protocols for preparation of chromatin from tissue and cells are different, performance of ChIP itself is similar for both. (pancreapedia.org)
  • Elements of stimulus-secretion coupling were studied in human pancreatic acinar cells by using tissue samples obtained from cadaver organ donors. (uab.edu)
  • The general underlying strategy behind the development of tissue engineered organ substitutes is the utilization of a combination of cells, biomaterials, and biochemical cues intended to recreate the natural organ environment. (nih.gov)
  • The purpose of this review is to highlight current bioengineering approaches for salivary gland tissue engineering and the adult stem cell sources used for this purpose. (nih.gov)
  • A) Amylase expressing cells (AC) in submandibular gland tissue (Tissue) were also present at the onset of culture (A-D0), and were visualized in the sphere at the onset of day 5 (A-D5), whereas granulae-containing spheres appeared in culture at later time-points (A-D10). (nih.gov)
  • Hes-1 was also expressed in the human tissue, with staining predominantly in the ductal cells. (uthscsa.edu)
  • In salivary tissue from rats undergoing and recovering from ductal obstruction, we found that Notch receptors and ligands were expressed in the nucleus of the regenerating epithelial cells. (uthscsa.edu)
  • suggesting that this gene is required not only for differentiation of acinar cells but also for their normal maintenance. (biologists.org)
  • Insulin protects rat pancreatic acinar cells from oxidant-induced inhibition of the plasma membrane calcium pump. (findaphd.com)
  • Baggaley E, Elliott AC & Bruce JIE (2008) Oxidant-induced inhibition of the plasma membrane Ca2+-ATPase in pancreatic acinar cells: Role of the mitochondria. (findaphd.com)
  • Laiho, K. U., Shelburne, J. D., Trump, B. F.: Observations on cell volume, ultrastructure, mitochondrial conformation and vital-dye uptake in Ehrlich ascites tumor cells: effects of inhibiting energy production and function of the plasma membrane. (springer.com)
  • The released Ca 2+ from these internal stores, in turn, stimulates the Ca 2+ -induced Ca 2+ influx through plasma membrane Ca 2+ channels ( 15 - 18 ) resulting in a transient and localized rise in [Ca 2+ ] cyto , mainly in the apical region of the cell. (pubmedcentralcanada.ca)
  • In patches of basolateral plasma membrane excised from acinar cell clusters, single-channel potassium currents were recorded. (uab.edu)
  • The mechanism by which Ca²⁺ can regulate enzyme secretion was investigated using Isolated intact pancreatic acinar cells and plasma membrane-enriched preparations obtained from these cells. (ubc.ca)
  • There are few known risk factors for the development of acinar cell neoplasms. (knowcancer.com)
  • Because there is no standard chemotherapy regimen for acinar cell neoplasms, patients should consider enrolling in a clinical trial of new chemotherapy or chemo-radiation strategies. (knowcancer.com)
  • Frequent expression has been documented in a number of neoplasms, such as hepatoblastoma, (117) undifferentiated embryonal sarcoma of the liver, (118) yolk sac tumor, (119, 120) choriocarcinoma, (121) pancreatic acinar cell carcinoma, (110, 121) Merkel cell carcinoma, (122) and lung squamous cell carcinoma. (thefreedictionary.com)
  • Although enforced expression of oncogenes under Elastase regulatory elements in the acinar compartments results in neoplasms with a mixed acinar-ductal histology ( 10 ), the nonphysiologic levels of transgene expression, the use of heterologous promoter elements, and the absence of appropriate precursor lesions recapitulating the cognate human disease renders extrapolation of these findings to the origins of human PDAC speculative at best. (pnas.org)
  • 3) Other stains (such as trypsin and chymotrypsin for pancreatic acinar cell carcinoma , chromogranin and synaptophysin for neuroendocrine neoplasms, and others) can be included in the panel, depending on the clinical information and the morphologic appearance. (thefreedictionary.com)
  • Whole-exome sequencing of pancreatic neoplasms with acinar differentiation. (semanticscholar.org)
  • Pancreatic ductal and acinar cell neoplasms in Carney complex: a possible new association. (cdc.gov)
  • More recently, studies have turned to determining effects of alcohol on the pancreatic duct cell, which is important for producing fluid secretion and carrying digestive enzymes secreted by the acinar cell into the gut lumen, where they are needed for meal digestion. (thefreedictionary.com)
  • It both synthesizes insulin, which manages our glucose levels and releases digestive cells into our intestine. (study.com)
  • Pancreatic acinar cells are the cells responsible for the synthesis, secretion, and storage of certain digestive enzymes. (study.com)
  • While digestive enzymes are secreted by the acinar cells, bicarbonate is secreted from the epithelial cells that line the small pancreatic ducts. (study.com)
  • Pancreatic acinar cells accumulate amino acids against a marked concentration gradient to synthesize digestive enzymes. (garvan.org.au)
  • Steroid treatment resulted in regeneration of acinar cells, accounting for restored digestive enzyme secretion. (elsevier.com)
  • Conclusions: Corticosteroids reduce inflammation and restore both digestive enzyme and HCO 3 - secretion in patients with AIP by regenerating acinar cells and correcting CFTR localization in pancreatic duct cells. (elsevier.com)
  • Here, we focus on acinar cell protein synthesis, trafficking, and processing in the pancreatic acinar cell necessary for its central role in producing digestive enzymes. (pancreapedia.org)
  • these cells are responsible for the production of the digestive enzymes. (wikipedia.org)
  • Normal stem cells give rise to progenitor cells that proliferate and differentiate into various types of mature cells, including [alpha]-cells, [beta]-cells, acinar cells , and ductal cells. (thefreedictionary.com)
  • An immediate need exists to obtain autologous adult progenitor cells as the use of embryonic and induced pluripotent stem cells potentially pose serious risks such as teratogenicity and immunogenic rejection. (rice.edu)
  • We conclude that the stem/progenitor potential of adult hS/PCs isolated without antigenic sorting or clonal expansion in suspension, combined with their ability to differentiate into specialized salivary cell lineages in a human-compatible culture system, makes them ideal for use in 3D bioengineered salivary gland applications. (rice.edu)
  • In mouse it was further found that an almost complete removal of acinar cells initiates regeneration from a currently not well-defined progenitor pool. (biologists.org)
  • Salivary gland progenitor cell biology provides a rationale for therapeutic salivary gland regeneration. (semanticscholar.org)
  • The Notch pathway is crucial for stem/progenitor cell maintenance, growth and differentiation in a variety of tissues. (uthscsa.edu)
  • However, another pancreatic secretagogue, bombesin, retained its ability to fully stimulate amylase secretion in acinar preparations from M 1 /M 3 receptor double KO mice. (aspetjournals.org)
  • We also provide a guide for assessing quality of the acinar preparation based on morphology and amylase secretion. (pancreapedia.org)
  • To address whether β-catenin remains important for the maintenance and expansion of mature acinar cells, we have established a system to follow the behavior and fate of β-catenin-deficient cells during postnatal growth and regeneration in mice. (biologists.org)
  • Acinar cells produce and store proteases, amylase and lipase, while ductal cells manufacture bicarbonate-rich fluid. (thefreedictionary.com)
  • Initial H&E, Periodic Acid Schiff (PAS), CK7, and CK14 staining showed that cultured spheres contained acinar and ductal cells. (nih.gov)
  • The question arises whether dexamethasone also has the capacity to induce ductal cells as well as hepatocytes. (bath.ac.uk)
  • Addition of dexamethasone inhibited pancreatic amylase expression, induced expression of the hepatocyte marker transferrin as well as markers typical of ductal cells: cytokeratin 7 and 19 and the lectin peanut agglutinin. (bath.ac.uk)
  • However, the number of ductal cells was low compared to hepatocytes. (bath.ac.uk)
  • The proportion of ductal cells was enhanced by culture with dexamethasone and epidermal growth factor (EGF). (bath.ac.uk)
  • We established several features of the mechanism underlying the transdifferentiation of pancreatic exocrine cells to ductal cells. (bath.ac.uk)
  • Using a CK19 promoter reporter, we show that a proportion of the ductal cells arise from differentiated pancreatic exocrine-like cells. (bath.ac.uk)
  • Conclusions/Significance: These results indicate that hepatocytes and ductal cells may be induced from pancreatic exocrine AR42J-B13 cells following treatment with dexamethasone. (bath.ac.uk)
  • The conversion from pancreatic to hepatocyte or ductal cells is dependent upon the expression of C/EBP beta. (bath.ac.uk)
  • We present results primarily obtained using rodent acinar cells, though the limited data from human acinar cells suggest the functions are likely the same as in rodents. (pancreapedia.org)
  • Given that normal acinar cells do not maintain the properties differentiated in vitro, the main aim of this research was to produce cells from undifferentiated precursors so that new study models could be developed. (imim.es)
  • We show that exposure of rodent acinar cells to ethanol induces protein kinase C-dependent Raf-1 kinase inhibitory protein phosphorylation, sensitization of cholecystokinin-stimulated Ca 2+ signaling, and potentiation of both basal and cholecystokinin-stimulated extracellular signal-regulated kinase activation. (pubmedcentralcanada.ca)
  • Further studies are necessary to correlate culture conditions and time course of biomarkers expression in order to follow lacrimal gland acinar cells proliferation, maintenance and function in culture. (arvojournals.org)
  • In summary, our results show that the media formulation, supplement profile, and extracellular matrix composition are important for maximal expression of androgen-induced effects by lacrimal gland acinar cells in vitro. (arvojournals.org)
  • The final histopathologic studies on the surgical specimen revealed an uncommon mixed acinar-ductal differentiation pattern. (oatext.com)
  • It is characterized by the presence of relatively uniform malignant cells which form acinar patterns. (linkedlifedata.com)
  • A malignant tumor arising from secreting cells of a racemose gland, particularly the salivary glands. (ucdenver.edu)
  • hAECs were co-cultured with submandibular gland acinar cells of SD rats using a double-chamber system. (semanticscholar.org)
  • GDI is introduced into chromaffin cells of rats through patch-clamp glass pipettes and increases in membrane capacitance was monitored. (nii.ac.jp)
  • Publications] Maruyama,Y.: 'Activation and desensitization mechanisms of muscarinic current responses in single pancreatic acinar cells of rats' Journal of physiology. (nii.ac.jp)
  • Publications] Maruyama,Y.: 'Inhibitory effects of arachidonic acid on muscarinic current response in single pancreatic acinar cells of rats. (nii.ac.jp)
  • American Journal of Physiology (Cell Physiology) 295(5): C1247-60. (findaphd.com)
  • Miller, LJ 1992, ' Pancreatic acinar cell physiology and function ', Current Opinion in Gastroenterology , vol. 8, no. 5, pp. 801-807. (elsevier.com)
  • American Journal of Physiology - Cell Physiology , 281 (1 50-1), C311-C319. (elsevier.com)
  • Cholecystokinin octapeptide (5 pM) also generates repetitive spikes, but a significant proportion of these trigger longer and larger pulses of Ca(2+)-dependent current and these waves can easily spread from cell to cell. (epfl.ch)
  • Important secretagogues such as ACh (acetylcholine) and CCK (cholecystokinin) utilize Ins P 3 and Ca 2+ signalling cascades to regulate secretion in these cells [ 2 ]. (biochemj.org)
  • In this study we identify Raf-1 kinase inhibitory protein as an essential mediator of ethanol-induced sensitization of cholecystokinin- and carbachol-regulated Ca 2+ signaling in pancreatic acinar cells. (pubmedcentralcanada.ca)
  • In this work, we have examined some mechanisms for desensitization of the cholecystokinin (CCK) receptor which is native to the pancreatic acinar cell, and have found the predominant mechanism to be distinct from these recognized processes. (rupress.org)
  • Way D, Smith S, Sivendran S, Chie L, Kanovsky M, Brandt-Rauf PW, Chung DL, Michl J, Pincus MR. A protein kinase C inhibitor induces phenotypic reversion of ras-transformed pancreatic cancer cells and cooperatively blocks tumor cell proliferation with an anti- ras peptide. (ucdenver.edu)
  • Compared with the SAP group, we found that pretreatment with aspirin markedly reduced the histological features of pancreatic injury in L-ASA and H-ASA group, characterized as lower degree of edema, less inflammatory cell infiltration, and alleviated acinar cell necrosis. (thefreedictionary.com)
  • Fibronectin expression modulates mammary epithelial cell proliferation during acinar differentiation. (semanticscholar.org)
  • The human salivary epithelial cell line, HSG, was studied to determine the role of Notch signaling in salivary epithelial cell differentiation. (uthscsa.edu)
  • The presence of a post-DNA-synthetic population is seen in the form of a group of nonlabeling central cells that remains intact in the midst of a labeled epithelium for as long as 48 hours in vitro (from 72 to 120 hours). (rupress.org)
  • Rat pancreatic slices were incubated at 37°C in vitro , in order to determine if complete ischemia would reproduce the subcellular alterations seen in human pancreatic acinar cells following shock. (springer.com)
  • The introduction of either a specific Rab27 binding domain (i.e. a recombinant Slp homology domain of Slac2-b that specifically binds Rab27A/B but not other Rabs) or functionally blocking antibodies that specifically disrupt Rab27B-Slac2-c complex in vitro strongly inhibited isoproterenol-stimulated amylase release from streptolysin O-permeabilized parotid acinar cells. (elsevier.com)
  • The mechanisms underlying such a leakage in cells of the submaxillary salivary gland were not studied. (springer.com)
  • Therefore, passive leakage of Ca 2+ from the ER in acinar cells of the submaxillary salivary gland is realized through pores of the translocon complex of the ER membrane. (springer.com)
  • Bone marrow-derived cells rescue salivary gland function in mice with head and neck irradiation. (semanticscholar.org)
  • Stem cells and the repair of radiation-induced salivary gland damage. (semanticscholar.org)
  • Taken together, these data suggest that Notch signaling is critical for normal salivary gland cell growth and differentiation. (uthscsa.edu)
  • Franz tumor, solid and papillary epithelial neoplasm (SPEN), solid and papillary tumor, solid and cystic tumor, papillary-cystic tumor, papillary cystic epithelial neoplasm, and solid and cystic acinar cell neoplasm are synonymous with SPT. (thefreedictionary.com)
  • A large fraction of the cystic fibrosis transmembrane conductance regulator (CFTR), which plays a central role in pancreatic duct HCO 3 - secretion, was mislocalized to the cytoplasm of duct cells before treatment. (elsevier.com)
  • 1. a type of basophilic cell that makes up most of the bulk of the islets of Langerhans and secretes insulin . (thefreedictionary.com)
  • Similarly, decreased concentrations or deletions of selected media supplements, including insulin, which binds to acinar cells, and dexamethasone, led to a significant diminution in the extent of androgen action, as well as to a decline in cell maintenance. (arvojournals.org)
  • These organelles are not observed to represent prominent compartments for the same receptor to traverse in the acinar cell, although fluorescent insulin is clearly internalized in these cells via receptor-mediated endocytosis. (rupress.org)
  • OBJECTIVE Regeneration of the insulin-secreting β-cells is a fundamental research goal that could benefit patients with either type 1 or type 2 diabetes. (diabetesjournals.org)
  • β-Cell mass normally grows well into adulthood to provide increased insulin secretion capacity to match the greater insulin requirements of maturity ( 1 , 2 ). (diabetesjournals.org)
  • β-Cell mass can slowly expand in adult rodents in response to increased insulin requirements ( 3 ) or during pregnancy ( 4 ). (diabetesjournals.org)
  • Arranged in rounded glands, these cells secrete an alkaline, enzyme rich fluid into the duodenum via the small duct (in blue) at top of image. (sciencephoto.com)
  • Using short-term lineage-tracing experiments and EdU incorporation assays, we exclude duct-associated Notch-responsive cells as the source of regeneration. (biologists.org)
  • The nature of these cells has not been clarified, although the data suggest involvement of duct or duct-associated cells. (biologists.org)
  • D = duct cells, AC = acinar cells, D0-3- 5-10 represent days in culture). (nih.gov)