Cells lining the saclike dilatations known as acini of various glands or the lungs.
A malignant tumor arising from secreting cells of a racemose gland, particularly the salivary glands. Racemose (Latin racemosus, full of clusters) refers, as does acinar (Latin acinus, grape), to small saclike dilatations in various glands. Acinar cell carcinomas are usually well differentiated and account for about 13% of the cancers arising in the parotid gland. Lymph node metastasis occurs in about 16% of cases. Local recurrences and distant metastases many years after treatment are common. This tumor appears in all age groups and is most common in women. (Stedman, 25th ed; Holland et al., Cancer Medicine, 3d ed, p1240; from DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, p575)
A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.
The major component (about 80%) of the PANCREAS composed of acinar functional units of tubular and spherical cells. The acinar cells synthesize and secrete several digestive enzymes such as TRYPSINOGEN; LIPASE; AMYLASE; and RIBONUCLEASE. Secretion from the exocrine pancreas drains into the pancreatic ductal system and empties into the DUODENUM.
The largest of the three pairs of SALIVARY GLANDS. They lie on the sides of the FACE immediately below and in front of the EAR.
A group of amylolytic enzymes that cleave starch, glycogen, and related alpha-1,4-glucans. (Stedman, 25th ed) EC 3.2.1.-.
A specific decapeptide obtained from the skin of Hila caerulea, an Australian amphibian. Caerulein is similar in action and composition to CHOLECYSTOKININ. It stimulates gastric, biliary, and pancreatic secretion; and certain smooth muscle. It is used in paralytic ileus and as diagnostic aid in pancreatic malfunction.
The tear-forming and tear-conducting system which includes the lacrimal glands, eyelid margins, conjunctival sac, and the tear drainage system.
A peptide, of about 33 amino acids, secreted by the upper INTESTINAL MUCOSA and also found in the central nervous system. It causes gallbladder contraction, release of pancreatic exocrine (or digestive) enzymes, and affects other gastrointestinal functions. Cholecystokinin may be the mediator of satiety.
One of two salivary glands in the neck, located in the space bound by the two bellies of the digastric muscle and the angle of the mandible. It discharges through the submandibular duct. The secretory units are predominantly serous although a few mucous alveoli, some with serous demilunes, occur. (Stedman, 25th ed)
INFLAMMATION of the PANCREAS. Pancreatitis is classified as acute unless there are computed tomographic or endoscopic retrograde cholangiopancreatographic findings of CHRONIC PANCREATITIS (International Symposium on Acute Pancreatitis, Atlanta, 1992). The two most common forms of acute pancreatitis are ALCOHOLIC PANCREATITIS and gallstone pancreatitis.
A slowly hydrolyzed CHOLINERGIC AGONIST that acts at both MUSCARINIC RECEPTORS and NICOTINIC RECEPTORS.
Glands that secrete SALIVA in the MOUTH. There are three pairs of salivary glands (PAROTID GLAND; SUBLINGUAL GLAND; SUBMANDIBULAR GLAND).
The inactive proenzyme of trypsin secreted by the pancreas, activated in the duodenum via cleavage by enteropeptidase. (Stedman, 25th ed)
An octapeptide hormone present in the intestine and brain. When secreted from the gastric mucosa, it stimulates the release of bile from the gallbladder and digestive enzymes from the pancreas.
Ducts that collect PANCREATIC JUICE from the PANCREAS and supply it to the DUODENUM.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
A salivary gland on each side of the mouth below the TONGUE.
Aquaporin 5 is a water-specific channel protein that is expressed primarily in alveolar, tracheal, and upper bronchial EPITHELIUM. It plays an important role in maintaining water HOMEOSTASIS in the LUNGS and may also regulate release of SALIVA and TEARS in the SALIVARY GLANDS and the LACRIMAL GLAND.
Any of the ducts which transport saliva. Salivary ducts include the parotid duct, the major and minor sublingual ducts, and the submandibular duct.
Cell surface proteins that bind cholecystokinin (CCK) with high affinity and trigger intracellular changes influencing the behavior of cells. Cholecystokinin receptors are activated by GASTRIN as well as by CCK-4; CCK-8; and CCK-33. Activation of these receptors evokes secretion of AMYLASE by pancreatic acinar cells, acid and PEPSIN by stomach mucosal cells, and contraction of the PYLORUS and GALLBLADDER. The role of the widespread CCK receptors in the central nervous system is not well understood.
A bile salt formed in the liver from lithocholic acid conjugation with taurine, usually as the sodium salt. It solubilizes fats for absorption and is itself absorbed. It is a cholagogue and choleretic.
Vesicles derived from the GOLGI APPARATUS containing material to be released at the cell surface.
Antibiotic substance produced by various Streptomyces species. It is an inhibitor of enzymatic activities that involve glutamine and is used as an antineoplastic and immunosuppressive agent.
Condensed areas of cellular material that may be bounded by a membrane.
Tumors or cancer of the PANCREAS. Depending on the types of ISLET CELLS present in the tumors, various hormones can be secreted: GLUCAGON from PANCREATIC ALPHA CELLS; INSULIN from PANCREATIC BETA CELLS; and SOMATOSTATIN from the SOMATOSTATIN-SECRETING CELLS. Most are malignant except the insulin-producing tumors (INSULINOMA).
Drugs that bind to and activate cholinergic receptors.
A neurotransmitter found at neuromuscular junctions, autonomic ganglia, parasympathetic effector junctions, a subset of sympathetic effector junctions, and at many sites in the central nervous system.
A severe form of acute INFLAMMATION of the PANCREAS characterized by one or more areas of NECROSIS in the pancreas with varying degree of involvement of the surrounding tissues or organ systems. Massive pancreatic necrosis may lead to DIABETES MELLITUS, and malabsorption.
A peptide hormone of about 27 amino acids from the duodenal mucosa that activates pancreatic secretion and lowers the blood sugar level. (USAN and the USP Dictionary of Drug Names, 1994, p597)
Cellular release of material within membrane-limited vesicles by fusion of the vesicles with the CELL MEMBRANE.
Glands of external secretion that release its secretions to the body's cavities, organs, or surface, through a duct.
Intracellular messenger formed by the action of phospholipase C on phosphatidylinositol 4,5-bisphosphate, which is one of the phospholipids that make up the cell membrane. Inositol 1,4,5-trisphosphate is released into the cytoplasm where it releases calcium ions from internal stores within the cell's endoplasmic reticulum. These calcium ions stimulate the activity of B kinase or calmodulin.
Signal transduction mechanisms whereby calcium mobilization (from outside the cell or from intracellular storage pools) to the cytoplasm is triggered by external stimuli. Calcium signals are often seen to propagate as waves, oscillations, spikes, sparks, or puffs. The calcium acts as an intracellular messenger by activating calcium-responsive proteins.
The fluid containing digestive enzymes secreted by the pancreas in response to food in the duodenum.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
The discharge of saliva from the SALIVARY GLANDS that keeps the mouth tissues moist and aids in digestion.
Physiologically inactive substances that can be converted to active enzymes.
A tetradecapeptide originally obtained from the skins of toads Bombina bombina and B. variegata. It is also an endogenous neurotransmitter in many animals including mammals. Bombesin affects vascular and other smooth muscle, gastric secretion, and renal circulation and function.
A sesquiterpene lactone found in roots of THAPSIA. It inhibits CA(2+)-TRANSPORTING ATPASE mediated uptake of CALCIUM into SARCOPLASMIC RETICULUM.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
INFLAMMATION of the PANCREAS that is characterized by recurring or persistent ABDOMINAL PAIN with or without STEATORRHEA or DIABETES MELLITUS. It is characterized by the irregular destruction of the pancreatic parenchyma which may be focal, segmental, or diffuse.
Drugs that bind to and activate muscarinic cholinergic receptors (RECEPTORS, MUSCARINIC). Muscarinic agonists are most commonly used when it is desirable to increase smooth muscle tone, especially in the GI tract, urinary bladder and the eye. They may also be used to reduce heart rate.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
2-Amino-4-(ethylthio)butyric acid. An antimetabolite and methionine antagonist that interferes with amino acid incorporation into proteins and with cellular ATP utilization. It also produces liver neoplasms.
Organic compounds which contain tin in the molecule. Used widely in industry and agriculture.
A fluorescent calcium chelating agent which is used to study intracellular calcium in tissues.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The removal or interruption of some part of the parasympathetic nervous system for therapeutic or research purposes.
Intracellular receptors that bind to INOSITOL 1,4,5-TRISPHOSPHATE and play an important role in its intracellular signaling. Inositol 1,4,5-trisphosphate receptors are calcium channels that release CALCIUM in response to increased levels of inositol 1,4,5-trisphosphate in the CYTOPLASM.
An alkaloid, originally from Atropa belladonna, but found in other plants, mainly SOLANACEAE. Hyoscyamine is the 3(S)-endo isomer of atropine.
A group of compounds that are derivatives of beta-methylacetylcholine (methacholine).
Pathological processes of the PANCREAS.
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
A genetically related subfamily of RAB GTP-BINDING PROTEINS involved in calcium-dependent EXOCYTOSIS. This enzyme was formerly listed as EC 3.6.1.47.
Isopropyl analog of EPINEPHRINE; beta-sympathomimetic that acts on the heart, bronchi, skeletal muscle, alimentary tract, etc. It is used mainly as bronchodilator and heart stimulant.
Cell membrane glycoproteins that form channels to selectively pass chloride ions. Nonselective blockers include FENAMATES; ETHACRYNIC ACID; and TAMOXIFEN.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Acute or chronic INFLAMMATION of the PANCREAS due to excessive ALCOHOL DRINKING. Alcoholic pancreatitis usually presents as an acute episode but it is a chronic progressive disease in alcoholics.
Chronic inflammatory and autoimmune disease in which the salivary and lacrimal glands undergo progressive destruction by lymphocytes and plasma cells resulting in decreased production of saliva and tears. The primary form, often called sicca syndrome, involves both KERATOCONJUNCTIVITIS SICCA and XEROSTOMIA. The secondary form includes, in addition, the presence of a connective tissue disease, usually rheumatoid arthritis.
Inorganic compounds derived from hydrochloric acid that contain the Cl- ion.
A number of different cardioactive glycosides obtained from Strophanthus species. OUABAIN is from S. gratus and CYMARINE from S. kombe. They are used like the digitalis glycosides.
A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.
A subclass of alpha-amylase ISOENZYMES that are secreted into PANCREATIC JUICE.
Carcinoma that arises from the PANCREATIC DUCTS. It accounts for the majority of cancers derived from the PANCREAS.
Proteins and peptides found in SALIVA and the SALIVARY GLANDS. Some salivary proteins such as ALPHA-AMYLASES are enzymes, but their composition varies in different individuals.
The fluid secreted by the lacrimal glands. This fluid moistens the CONJUNCTIVA and CORNEA.
An eleven-amino acid neurotransmitter that appears in both the central and peripheral nervous systems. It is involved in transmission of PAIN, causes rapid contractions of the gastrointestinal smooth muscle, and modulates inflammatory and immune responses.
Inflammation of the lacrimal sac. (Dorland, 27th ed)
The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).
A subtype of cholecystokinin receptor found primarily in the PANCREAS; STOMACH; INTESTINE; and GALLBLADDER. It plays a role in regulating digestive functions such as gallbladder contraction, pancreatic enzyme secretion and absorption in the GASTROINTESTINAL TRACT.
Disease having a short and relatively severe course.
The clear, viscous fluid secreted by the SALIVARY GLANDS and mucous glands of the mouth. It contains MUCINS, water, organic salts, and ptylin.
The extracellular moiety of the POLYMERIC IMMUNOGLOBULIN RECEPTOR found alone or complexed with IGA or IGM, in a variety of external secretions (tears, bile, colostrum.) Secretory component is derived by proteolytic cleavage of the receptor during transcytosis. When immunoglobulins IgA and IgM are bound to the receptor, during their transcytosis secretory component becomes covalently attached to them generating SECRETORY IMMUNOGLOBULIN A or secretory IMMUNOGLOBULIN M.
Secretory cells of the ductless glands. They secrete HORMONES directly into the blood circulation (internal secretion) to be carried to the target cells. The secreted chemicals can be PEPTIDES; STEROIDS; NEUROPEPTIDES; or BIOGENIC AMINES.
Elements of limited time intervals, contributing to particular results or situations.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
A pyridine nucleotide that mobilizes CALCIUM. It is synthesized from nicotinamide adenine dinucleotide (NAD) by ADP RIBOSE CYCLASE.
Inorganic compounds that contain ruthenium as an integral part of the molecule.
Star-shaped, myofibroblast-like cells located in the periacinar, perivascular, and periductal regions of the EXOCRINE PANCREAS. They play a key role in the pathobiology of FIBROSIS; PANCREATITIS; and PANCREATIC CANCER.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
An enzyme of the hydrolase class that catalyzes the reaction of triacylglycerol and water to yield diacylglycerol and a fatty acid anion. It is produced by glands on the tongue and by the pancreas and initiates the digestion of dietary fats. (From Dorland, 27th ed) EC 3.1.1.3.
The proteinaceous component of the pancreatic stone in patients with PANCREATITIS.
A major class of calcium-activated potassium channels that were originally discovered in ERYTHROCYTES. They are found primarily in non-excitable CELLS and set up electrical gradients for PASSIVE ION TRANSPORT.
A chelating agent relatively more specific for calcium and less toxic than EDETIC ACID.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Phosphoric acid esters of inositol. They include mono- and polyphosphoric acid esters, with the exception of inositol hexaphosphate which is PHYTIC ACID.
The amount of a substance secreted by cells or by a specific organ or organism over a given period of time; usually applies to those substances which are formed by glandular tissues and are released by them into biological fluids, e.g., secretory rate of corticosteroids by the adrenal cortex, secretory rate of gastric acid by the gastric mucosa.
The rate dynamics in chemical or physical systems.
An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.
The physiological renewal, repair, or replacement of tissue.
N-(1-Oxobutyl)-cyclic 3',5'-(hydrogen phosphate)-2'-butanoate guanosine. A derivative of cyclic GMP. It has a higher resistance to extracellular and intracellular phosphodiesterase than cyclic GMP.
Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN.
A sulfamyl diuretic.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
An analog of benzilylcholine mustard. It is an alkylating nitrogen mustard analog that binds specifically and irreversibly to cholinergic muscarinic receptors and is used as an affinity label to isolate and study the receptors.
A ubiquitously expressed protein kinase that is involved in a variety of cellular SIGNAL PATHWAYS. Its activity is regulated by a variety of signaling protein tyrosine kinase.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
A class of compounds composed of repeating 5-carbon units of HEMITERPENES.
A widely distributed purinergic P2X receptor subtype that plays a role in pain sensation. P2X4 receptors found on MICROGLIA cells may also play a role in the mediation of allodynia-related NEUROPATHIC PAIN.
A highly basic, 28 amino acid neuropeptide released from intestinal mucosa. It has a wide range of biological actions affecting the cardiovascular, gastrointestinal, and respiratory systems and is neuroprotective. It binds special receptors (RECEPTORS, VASOACTIVE INTESTINAL PEPTIDE).
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.
A serine proteinase inhibitor used therapeutically in the treatment of pancreatitis, disseminated intravascular coagulation (DIC), and as a regional anticoagulant for hemodialysis. The drug inhibits the hydrolytic effects of thrombin, plasmin, and kallikrein, but not of chymotrypsin and aprotinin.
An oligopeptide isolated from the skin of Physalaemus fuscumaculatus, a South American frog. It is a typical kinin, resembling SUBSTANCE P in structure and action and has been proposed as a sialagogue, antihypertensive, and vasodilator.
A type of endoplasmic reticulum (ER) where polyribosomes are present on the cytoplasmic surfaces of the ER membranes. This form of ER is prominent in cells specialized for protein secretion and its principal function is to segregate proteins destined for export or intracellular utilization.
A derivative of benzodiazepine that acts on the cholecystokinin A (CCKA) receptor to antagonize CCK-8's (SINCALIDE) physiological and behavioral effects, such as pancreatic stimulation and inhibition of feeding.
A protease of broad specificity, obtained from dried pancreas. Molecular weight is approximately 25,000. The enzyme breaks down elastin, the specific protein of elastic fibers, and digests other proteins such as fibrin, hemoglobin, and albumin. EC 3.4.21.36.
Gastrointestinal agents that stimulate the flow of bile into the duodenum (cholagogues) or stimulate the production of bile by the liver (choleretic).
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
A cyclic nucleotide derivative that mimics the action of endogenous CYCLIC AMP and is capable of permeating the cell membrane. It has vasodilator properties and is used as a cardiac stimulant. (From Merck Index, 11th ed)
The pathological process occurring in cells that are dying from irreparable injuries. It is caused by the progressive, uncontrolled action of degradative ENZYMES, leading to MITOCHONDRIAL SWELLING, nuclear flocculation, and cell lysis. It is distinct it from APOPTOSIS, which is a normal, regulated cellular process.
HORMONES secreted by the gastrointestinal mucosa that affect the timing or the quality of secretion of digestive enzymes, and regulate the motor activity of the digestive system organs.
Voltage-dependent cell membrane glycoproteins selectively permeable to calcium ions. They are categorized as L-, T-, N-, P-, Q-, and R-types based on the activation and inactivation kinetics, ion specificity, and sensitivity to drugs and toxins. The L- and T-types are present throughout the cardiovascular and central nervous systems and the N-, P-, Q-, & R-types are located in neuronal tissue.
SNARE proteins where the central amino acid residue of the SNARE motif is an ARGININE. They are classified separately from the Q-SNARE PROTEINS where the central amino acid residue of the SNARE motif is a GLUTAMINE. This subfamily contains the vesicle associated membrane proteins (VAMPs) based on similarity to the prototype for the R-SNAREs, VAMP2 (synaptobrevin 2).
A naturally occurring phenomenon where terminally differentiated cells dedifferentiate to the point where they can switch CELL LINEAGES. The cells then differentiate into other cell types.
A primary malignant neoplasm of the pancreatic ISLET CELLS. Usually it involves the non-INSULIN-producing cell types, the PANCREATIC ALPHA CELLS and the pancreatic delta cells (SOMATOSTATIN-SECRETING CELLS) in GLUCAGONOMA and SOMATOSTATINOMA, respectively.
The abundant submucosal mucous glands in the DUODENUM. These glands secrete BICARBONATE IONS; GLYCOPROTEINS; and PEPSINOGEN II.
Chelating agent used for heavy metal poisoning and assay. It causes diabetes.
A benign neoplasm derived from glandular epithelium, in which cystic accumulations of retained secretions are formed. In some instances, considerable portions of the neoplasm, or even the entire mass, may be cystic. (Stedman, 25th ed)
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
Carboxypeptidases that are primarily found the DIGESTIVE SYSTEM that catalyze the release of C-terminal amino acids. Carboxypeptidases A have little or no activity for hydrolysis of C-terminal ASPARTIC ACID; GLUTAMIC ACID; ARGININE; LYSINE; or PROLINE. This enzyme requires ZINC as a cofactor and was formerly listed as EC 3.4.2.1 and EC 3.4.12.2.
A condition in which there is a change of one adult cell type to another similar adult cell type.
A bile salt formed in the liver by conjugation of chenodeoxycholate with taurine, usually as the sodium salt. It acts as detergent to solubilize fats in the small intestine and is itself absorbed. It is used as a cholagogue and choleretic.
A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.
The fluid inside CELLS.
Inorganic salts that contain the -HCO3 radical. They are an important factor in determining the pH of the blood and the concentration of bicarbonate ions is regulated by the kidney. Levels in the blood are an index of the alkali reserve or buffering capacity.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
The ability of a substrate to allow the passage of ELECTRONS.
The increase in a measurable parameter of a PHYSIOLOGICAL PROCESS, including cellular, microbial, and plant; immunological, cardiovascular, respiratory, reproductive, urinary, digestive, neural, musculoskeletal, ocular, and skin physiological processes; or METABOLIC PROCESS, including enzymatic and other pharmacological processes, by a drug or other chemical.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
A serine endopeptidase secreted by the pancreas as its zymogen, CHYMOTRYPSINOGEN and carried in the pancreatic juice to the duodenum where it is activated by TRYPSIN. It selectively cleaves aromatic amino acids on the carboxyl side.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
The craniosacral division of the autonomic nervous system. The cell bodies of the parasympathetic preganglionic fibers are in brain stem nuclei and in the sacral spinal cord. They synapse in cranial autonomic ganglia or in terminal ganglia near target organs. The parasympathetic nervous system generally acts to conserve resources and restore homeostasis, often with effects reciprocal to the sympathetic nervous system.
Cation-transporting proteins that utilize the energy of ATP hydrolysis for the transport of CALCIUM. They differ from CALCIUM CHANNELS which allow calcium to pass through a membrane without the use of energy.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
A subclass of symporters that specifically transport SODIUM CHLORIDE and/or POTASSIUM CHLORIDE across cellular membranes in a tightly coupled process.
A quaternary ammonium parasympathomimetic agent with the muscarinic actions of ACETYLCHOLINE. It is hydrolyzed by ACETYLCHOLINESTERASE at a considerably slower rate than ACETYLCHOLINE and is more resistant to hydrolysis by nonspecific CHOLINESTERASES so that its actions are more prolonged. It is used as a parasympathomimetic bronchoconstrictor agent and as a diagnostic aid for bronchial asthma. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1116)
A group of compounds with the heterocyclic ring structure of benzo(c)pyridine. The ring structure is characteristic of the group of opium alkaloids such as papaverine. (From Stedman, 25th ed)
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Insects of the order Dictyoptera comprising several families including Blaberidae, BLATTELLIDAE, Blattidae (containing the American cockroach PERIPLANETA americana), Cryptocercidae, and Polyphagidae.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Exotoxins produced by certain strains of streptococci, particularly those of group A (STREPTOCOCCUS PYOGENES), that cause HEMOLYSIS.
A family of DNA-binding transcription factors that contain a basic HELIX-LOOP-HELIX MOTIF.
A purinergic P2X neurotransmitter receptor that plays a role in pain sensation signaling and regulation of inflammatory processes.
Chemical bond cleavage reactions resulting from absorption of radiant energy.
The movement of ions across energy-transducing cell membranes. Transport can be active, passive or facilitated. Ions may travel by themselves (uniport), or as a group of two or more ions in the same (symport) or opposite (antiport) directions.
A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition.
Compounds that contain a BENZENE ring fused to a furan ring.
An inorganic dye used in microscopy for differential staining and as a diagnostic reagent. In research this compound is used to study changes in cytoplasmic concentrations of calcium. Ruthenium red inhibits calcium transport through membrane channels.
A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.
A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)
An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.
A colorless, syrupy, strongly acidic liquid that can form detergents with oleic acid.
An ionophorous, polyether antibiotic from Streptomyces chartreusensis. It binds and transports CALCIUM and other divalent cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. The substance is used mostly as a biochemical tool to study the role of divalent cations in various biological systems.
A ZINC-dependent carboxypeptidase primary found in the DIGESTIVE SYSTEM. The enzyme catalyzes the preferential cleavage of a C-terminal peptidyl-L-lysine or arginine. It was formerly classified as EC 3.4.2.2 and EC 3.4.12.3.
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
A plasma membrane exchange glycoprotein transporter that functions in intracellular pH regulation, cell volume regulation, and cellular response to many different hormones and mitogens.
Potent activator of the adenylate cyclase system and the biosynthesis of cyclic AMP. From the plant COLEUS FORSKOHLII. Has antihypertensive, positive inotropic, platelet aggregation inhibitory, and smooth muscle relaxant activities; also lowers intraocular pressure and promotes release of hormones from the pituitary gland.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
A methylpyrrole-carboxylate from RYANIA that disrupts the RYANODINE RECEPTOR CALCIUM RELEASE CHANNEL to modify CALCIUM release from SARCOPLASMIC RETICULUM resulting in alteration of MUSCLE CONTRACTION. It was previously used in INSECTICIDES. It is used experimentally in conjunction with THAPSIGARGIN and other inhibitors of CALCIUM ATPASE uptake of calcium into SARCOPLASMIC RETICULUM.
A lysosomal cysteine proteinase with a specificity similar to that of PAPAIN. The enzyme is present in a variety of tissues and is important in many physiological and pathological processes. In pathology, cathepsin B has been found to be involved in DEMYELINATION; EMPHYSEMA; RHEUMATOID ARTHRITIS, and NEOPLASM INVASIVENESS.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A family of gastrointestinal peptide hormones that excite the secretion of GASTRIC JUICE. They may also occur in the central nervous system where they are presumed to be neurotransmitters.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
A slowly hydrolyzed muscarinic agonist with no nicotinic effects. Pilocarpine is used as a miotic and in the treatment of glaucoma.
A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.
Ubiquitous, inducible, nuclear transcriptional activator that binds to enhancer elements in many different cell types and is activated by pathogenic stimuli. The NF-kappa B complex is a heterodimer composed of two DNA-binding subunits: NF-kappa B1 and relA.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
L-Tryptophyl-L-methionyl-L-aspartyl-L-phenylalaninamide. The C-terminal tetrapeptide of gastrin. It is the smallest peptide fragment of gastrin which has the same physiological and pharmacological activity as gastrin.
The measurement of an organ in volume, mass, or heaviness.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed)
An ester formed between the aldehydic carbon of RIBOSE and the terminal phosphate of ADENOSINE DIPHOSPHATE. It is produced by the hydrolysis of nicotinamide-adenine dinucleotide (NAD) by a variety of enzymes, some of which transfer an ADP-ribosyl group to target proteins.
Agents that inhibit SODIUM-POTASSIUM-CHLORIDE SYMPORTERS which are concentrated in the thick ascending limb at the junction of the LOOP OF HENLE and KIDNEY TUBULES, DISTAL. They act as DIURETICS. Excess use is associated with HYPOKALEMIA and HYPERGLYCEMIA.
A drug that exerts an inhibitory effect on gastric secretion and reduces gastrointestinal motility. It is used clinically in the drug therapy of gastrointestinal ulcers.
A synthetic pentapeptide that has effects like gastrin when given parenterally. It stimulates the secretion of gastric acid, pepsin, and intrinsic factor, and has been used as a diagnostic aid.
A type of glycoside widely distributed in plants. Each consists of a sapogenin as the aglycone moiety, and a sugar. The sapogenin may be a steroid or a triterpene and the sugar may be glucose, galactose, a pentose, or a methylpentose.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
A slowly hydrolyzing muscarinic agonist with no nicotinic effects. Bethanechol is generally used to increase smooth muscle tone, as in the GI tract following abdominal surgery or in urinary retention in the absence of obstruction. It may cause hypotension, HEART RATE changes, and BRONCHIAL SPASM.
A type of pancreatic cell representing about 50-80% of the islet cells. Beta cells secrete INSULIN.
Compounds with a core of fused benzo-pyran rings.
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)

Recovery of INS-R and ER-alpha expression in the salivary glands of diabetic mice submitted to hormone replacement therapy. (1/157)

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Histopathology and ex vivo insulin secretion of pancreatic islets in gestational diabetes: A case report. (2/157)

Gestational diabetes (GD) results from insufficient endogenous insulin supply. No information is available on features of islet cells in human GD. Herein, we describe several properties of islets from a woman with GD. Immunohistochemical stainings and EM analyses were performed on pancreatic samples. Islet isolation was achieved by enzymatic dissociation and density gradient centrifugation. Ex vivo insulin secretion was studied in response to fuel secretagogues. Control islets were obtained from matched non-pregnant, non-diabetic women. Total insulin positive area was lower in GD, mainly due to the presence of smaller islets. beta-cell apoptosis and the presence of Ki67 positive islet cells were similar in GD and controls, whereas the amount of insulin positive cells in or close to the ducts was decreased in GD. Ex vivo insulin secretion did not differ between GD and non-pregnant, non-diabetic islets. These findings suggest that in this case of human GD there might mainly be a defect of beta-cell amount, not due to increased apoptosis, but possibly to insufficient regeneration.  (+info)

Tauroursodeoxycholic acid reduces endoplasmic reticulum stress, acinar cell damage, and systemic inflammation in acute pancreatitis. (3/157)

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PKCtheta activation in pancreatic acinar cells by gastrointestinal hormones/neurotransmitters and growth factors is needed for stimulation of numerous important cellular signaling cascades. (4/157)

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Role of neutrophils in the activation of trypsinogen in severe acute pancreatitis. (5/157)

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Functional role of an islet transcription factor, INSM1/IA-1, on pancreatic acinar cell trans-differentiation. (6/157)

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Secretin is not necessary for exocrine pancreatic development and growth in mice. (7/157)

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The distribution of phosphatidylinositol 4,5-bisphosphate in acinar cells of rat pancreas revealed with the freeze-fracture replica labeling method. (8/157)

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TY - JOUR. T1 - Matrix metalloproteinase-7 is expressed by pancreatic cancer precursors and regulates acinar-to-ductal metaplasia in exocrine pancreas. AU - Crawford, Howard C.. AU - Scoggins, Charles R.. AU - Kay Washington, M.. AU - Matrisian, Lynn M.. AU - Leach, Steven D.. PY - 2002. Y1 - 2002. N2 - In gastrointestinal epithelium, metaplastic conversion between predominant cell types is associated with an increased risk of neoplasia. However, the mechanisms regulating metaplastic transitions in adult epithelia are largely undefined. Here we show that matrix metalloproteinase-7 (MMP-7) is expressed not only in the majority of human pancreatic ductal adenocarcinoma specimens, but also in human pancreatic intraepithelial neoplasia and metaplastic duct lesions in human and mouse. In a mouse model of pancreatic acinar-to-ductal metaplasia, MMP-7 progressively accumulates during the metaplastic transition, resulting in a concomitant increase in solubilization of Fas ligand (FasL). Under identical ...
Definition of acinar cell in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is acinar cell? Meaning of acinar cell as a legal term. What does acinar cell mean in law?
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TY - JOUR. T1 - Cancer-associated acinar-to-ductal metaplasia within the invasive front of pancreatic cancer contributes to local invasion. AU - Kibe, Shin. AU - Ohuchida, Kenoki. AU - Ando, Yohei. AU - Takesue, Shin. AU - Nakayama, Hiromichi. AU - Abe, Toshiya. AU - Endo, Sho. AU - Koikawa, Kazuhiro. AU - Okumura, Takashi. AU - Iwamoto, Chika. AU - Shindo, Koji. AU - Moriyama, Taiki. AU - Nakata, Kohei. AU - Miyasaka, Yoshihiro. AU - Shimamoto, Masaya. AU - Ohtsuka, Takao. AU - Mizumoto, Kazuhiro. AU - Oda, Yoshinao. AU - Nakamura, Masafumi. N1 - Funding Information: This work was supported in part by JSPS KAKENHI (Grant Numbers JP16H05418, JP16K10601, JP17H04284, JP17K19602, JP17K19605, JP18K07943).The authors thank E. Manabe, S. Sadatomi, N. Torada (Department of Surgery and Oncology, Kyushu University Hospital), and members of the Research Support Center and Department of Anatomic Pathology, Graduate School of Medical Sciences, Kyushu University for their expert technical assistance. The ...
Cancer subtype switching, which involves unclear cancer cell origin, cell fate decision, and transdifferentiation of cells within a confined tumor microenvironment, remains a major problem in pancreatic cancer (PDA). By analyzing PDA subtypes in The Cancer Genome Atlas, we identified that epigenetic silencing of apoptosis-associated tyrosine kinase (AATK) inversely was correlated with mRNA expression and was enriched in the quasi-mesenchymal cancer subtype. By comparing early mouse pancreatic lesions, the non-invasive regions showed AATK co-expression in cells with acinar-to-ductal metaplasia, nuclear VAV1 localization, and cell cycle suppression; but the invasive lesions conversely revealed diminished AATK expression in those with poorly differentiated histology, cytosolic VAV1 localization, and co-expression of p63 and HNF1α. Transiently activated AATK initiates acinar differentiation into a ductal cell fate to establish apical-basal polarization in acinar-to-ductal metaplasia. Silenced AATK and
Define acinar cell, acinous cell. acinar cell, acinous cell synonyms, acinar cell, acinous cell pronunciation, acinar cell, acinous cell translation, English dictionary definition of acinar cell, acinous cell. a small room as in a convent or prison; basic structural unit of all organisms: a one-celled animal Not to be confused with: sell - to transfer goods or...
https://doi.org/10.18632/oncotarget.2041 Parama Dey, Satyanarayana Rachagani, Arokia P. Vaz, Moorthy P. Ponnusamy, Surinder K. Batra
TY - JOUR. T1 - Pancreatic acinar cell physiology and function. AU - Miller, L. J.. PY - 1992/1/1. Y1 - 1992/1/1. N2 - The past year has witnessed a marked increase in the complexity of intracellular processes involved in the function of a polarized epithelial cell. The pancreatic acinar cell is perhaps the best studied such cell, and the classical model for stimulus-secretion coupling and secretory protein biosynthesis and export. In this review, I examine several new reports that impact on these themes. I include the identification of newly recognized pancreatic secretagogues; insights into the biochemical basis for hormone receptor affinity states and interconversion, desensitization, and internalization; lipid mediators of secretagogue action; patterns and regulators of intracellular calcium events along with their spatial and temporal patterns; regulated channels, transporters, and junctional communication in the acinar cell and cellular organelles; and new insights into regulated ...
Acinar transdifferentiation toward a duct-like phenotype constitutes the defining response of acinar cells to external stress signals and is considered to be the initial step in pancreatic carcinogenesis. Despite the requirement for oncogenic Kras in pancreatic cancer (PDAC) development, oncogenic Kras is not sufficient to drive pancreatic carcinogenesis beyond the level of premalignancy. Instead, secondary events, such as inflammation-induced signaling activation of the epidermal growth factor (EGFR) or induction of Sox9 expression, are required for tumor formation. Herein, we aimed to dissect the mechanism that links EGFR signaling to Sox9 gene expression during acinar-to-ductal metaplasia in pancreatic tissue adaptation and PDAC initiation. We show that the inflammatory transcription factor NFATc4 is highly induced and localizes in the nucleus in response to inflammation-induced EGFR signaling. Moreover, we demonstrate that NFATc4 drives acinar-to-ductal conversion and PDAC initiation through direct
B. DNA-Protein Cross-Linking. To cross-link protein to the genomic DNA, 270 µl of formalin was added to 10 ml of DMEM containing acini to obtain a final solution of 1% formaldehyde. Cells were agitated for 10 minutes (Note 1) on a shaker table. Following the addition of 514 µl of 2.5 M glycine (125 mM final) for 5 minutes to quench the formaldehyde and terminate the crosslinking reaction, cells were centrifuged at 2000 rpm for 3 minutes at 40C. The pellet of acinar cells was washed twice with ice cold PBS.. C. Isolation of Nuclei from Isolated Acinar Cells. To isolate nuclei, acinar cells were incubated in Cytoplasmic Extract buffer (CE Buffer; 10 mM HEPES pH 7.4, 10 mM KCl, 1.5 mM MgCl2, 0.1% Triton-X100, 0.5 mM DTT and protease inhibitors [5 µg/mL Aprotinin, 5 µg/mL Leupeptin, 5 µg/mL Pepstatin, 75 µg/mL PMSF]) followed by 5 strokes of Potter-Elvehjem homogenizer. The cells were set on ice for 15 minutes. To pellet the nuclei, the samples were centrifuged at 4oC and 5000 g for 10 ...
title: Suppression of NF-kappaB activation and cytokine production by N-acetylcysteine in pancreatic acinar cells., doi: 10.1016/S0891-5849(00)00368-3, category: Article
As multiple recent studies have described acinar cells undergoing acinar to ductal metaplasia as a common cell of origin of PDAC (and reviewed in the study by Rooman and Real) the central role of MYC in acinar cells within the pancreas is of particular ...
Interpretive Summary: The establishment and initial characterization of a pig embryonic stem cell-derived pancreatic cell line, PICM-31, is described. The PICM-31 cell line was started from the in vitro culture of the inner cells of an early pig embryo. The cells were small and grew relatively slowly. They frequently self-organized into radially symmetrical groupings of cells, i.e., wheel-like and similar to the acinar structures inside the pancreas. Electron microscopic examination confirmed that the cells were organized into a functional acinar-like unit and that they contained secretory granules as expected of the cells of the pancreas that secrete digestive enzymes. The PICM-31 cells were shown to be expressing the genes for digestive enzymes, and the secreted enzymes themselves were also identified by mass spectroscopy. The PICM-31 cells also expressed marker genes that indicated the cells may be progenitor pancreas cells, i.e., that the PICM-31 cells might be able to change into the ...
To protect acinar cells from self-digestion, the powerful digestive enzymes that the cells make are initially produced in an inactive form, notes Pancreas.org. These inert enzymes are converted into...
Differentiation of salisphere into acinar cells. (A) Amylase expressing cells (AC) in submandibular gland tissue (Tissue) were also present at the onset of cult
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Agonist-specific cytosolic Ca2+ oscillation patterns can be observed in individual cells and these have been explained by the co-existence of separate oscillatory mechanisms. In pancreatic acinar cells activation of muscarinic receptors typically evokes sinusoidal oscillations whereas stimulation of cholecystokinin (CCK) receptors evokes transient oscillations consisting of Ca2+ waves with long intervals between them. We have monitored changes in the cytosolic Ca2+ concentration ([Ca2+]i) by measuring Ca2(+)-activated Cl- currents in single internally perfused mouse pancreatic acinar cells. With minimal intracellular Ca2+ buffering we found that low concentrations of both ACh (50 nM) and CCK (10 pM) evoked repetitive short-lasting Ca2+ spikes of the same duration and frequency, but the probability of a spike being followed by a longer and larger Ca2+ wave was low for ACh and high for CCK. The probability that the receptor-evoked shortlasting Ca2+ spikes would initiate more substantial Ca2+ waves was
2. Activation of ERK pathway in Pancreatic Acinar Cells (Figure 1). ERK activation is usually monitored by following the dual phosphorylation of the Thr and Tyr residues in the Thr-Glu-Tyr activation sequence brought about by MEK as there are a number of good phosphospecific antibodies directed at this epitope. It can also be shown by phosphorylation of myelin basic protein either in a test tube or by an in gel technique following gel electrophoresis and renaturization. Both Western blots and the in gel kinase procedure reveal the two forms of ERK at approximately 44 and 42 kDa; in fact, the molecules were originally referred to as p42 and p44 MAPK with p42 being what is now referred to as ERK2 and p44 now being ERK1. Using isolated rat or mouse pancreatic acini in vitro, ERK1/2 is activated by CCK, bombesin, substance P, and carbachol, all of which activate G protein coupled receptors coupled to Gq and calcium mobilization but not by secretin or VIP which activate receptors coupled to Gs and ...
Pieces of mouse embryonic pancreatic epithelium cultured in an inductive situation in vitro, or when examined at critical times in vivo, show a gradient of zymogen granule accumulation. Cells located internally in explants, or in central acini in vivo, show this overt differentiation first. As the epithelia age, the more peripheral cell population proceeds in a similar differentiation. Observations of autoradiograms of H3-thymidine-labeled tissues indicate that the first cells which cease incorporating the DNA-precursor are in the central regions that differentiate first. In older explants, thymidine incorporation is largely restricted to the periphery of the tissue as zymogen appears in the internal cells. Evidence suggests that cells or nuclei which have replicated DNA move inward before dividing. Some daughter cells apparently return peripherad to divide again, whereas others remain centrally where they undergo differentiation. During at least the first 24 hours of these maturational changes, ...
With the exception of a few types of cells, (acinar cells, T lymphocytes and hepatocytes), every cell in your body has a cilia. In the vision community, we are used to seeing these structures in the distal portion of the photoreceptors. The reality is that every cell in the retina has a cilium and some cells […]. ...
TY - JOUR. T1 - Genome-wide genetic and epigenetic analyses of pancreatic acinar cell carcinomas reveal aberrations in genome stability. AU - Jäkel, Cornelia. AU - Bergmann, Frank. AU - Toth, Reka. AU - Assenov, Yassen. AU - Van Der Duin, Daniel. AU - Strobel, Oliver. AU - Hank, Thomas. AU - Klöppel, Günter. AU - Dorrell, Craig. AU - Grompe, Markus. AU - Moss, Joshua. AU - Dor, Yuval. AU - Schirmacher, Peter. AU - Plass, Christoph. AU - Popanda, Odilia. AU - Schmezer, Peter. PY - 2017/12/1. Y1 - 2017/12/1. N2 - Pancreatic acinar cell carcinoma (ACC) is an aggressive exocrine tumor with largely unknown biology. Here, to identify potential targets for personalized treatment, we perform integrative genome-wide and epigenome-wide analyses. The results show frequently aberrant DNA methylation, abundant chromosomal amplifications and deletions, and mutational signatures suggesting defective DNA repair. In contrast to pancreatic ductal adenocarcinoma, no recurrent point mutations are detected. The ...
RESULTS: We defined three distinctive phases-termed inflammation, regeneration and refinement-following induction of moderate acute pancreatitis in wild-type mice. These corresponded to different waves of proliferation of mesenchymal, progenitor-like and acinar cells. Pancreas regeneration required a coordinated transition of proliferation between progenitor-like and acinar cells. In mice harbouring an oncogenic Kras mutation and challenged with pancreatitis, there was an extended inflammatory phase and a parallel, continuous proliferation of mesenchymal, progenitor-like and acinar cells. Analysis of high-resolution transcriptional data from wild-type animals revealed that organ regeneration relied on a complex interaction of a gene network that normally governs acinar cell homeostasis, exocrine specification and intercellular signalling. In mice with oncogenic Kras, a specific carcinogenic signature was found, which was preserved in full-blown mouse pancreas cancer ...
TY - JOUR. T1 - NFATc4 Regulates Sox9 gene expression in acinar cell plasticity and pancreatic cancer initiation. AU - Hessmann, Elisabeth. AU - Zhang, Jin San. AU - Chen, Nai Ming. AU - Hasselluhn, Marie. AU - Liou, Geou Yarh. AU - Storz, Peter. AU - Ellenrieder, Volker. AU - Billadeau, Daniel D.. AU - Koenig, Alexander. N1 - Publisher Copyright: © 2016 Elisabeth Hessmann et al.. PY - 2016. Y1 - 2016. N2 - Acinar transdifferentiation toward a duct-like phenotype constitutes the defining response of acinar cells to external stress signals and is considered to be the initial step in pancreatic carcinogenesis. Despite the requirement for oncogenic Kras in pancreatic cancer (PDAC) development, oncogenic Kras is not sufficient to drive pancreatic carcinogenesis beyond the level of premalignancy. Instead, secondary events, such as inflammation-induced signaling activation of the epidermal growth factor (EGFR) or induction of Sox9 expression, are required for tumor formation. Herein, we aimed to ...
In the pig parotid gland, ATPDase was mainly associated with duct epithelium, with very little, if any, immunoreactivity on acinar cells. Myoepithelial cells dispersed among acini produced a significant signal. There have been reports of Ca2+-dependent ATPases associated with isolated parotid acinar cells, but as judged by their biochemical properties, these ATPases are different from ATPDases. However, a nucleoside triphosphatase described by Sato et al. (29) in bovine parotid gland and an apyrase described by Valenzuela et al. (34) in a microsomal fraction of rat salivary gland appear to correspond to the ATPDase described in this study and hence could well be associated with ductal epithelial and myoepithelial cells. In agreement with these findings, some studies on rat parotid acini show very little ecto-ATPase activity (9). Some P2Zpurinoceptors have been described on rat parotid acinar cells, which respond to ATP and mediate a Ca2+increase caused by both an influx and a mobilization from ...
We hypothesize, due to the Sjögrens syndrome (SS) disease characteristics, that the primary target hit by the disease process is the secretory acinar cell and that this cell is particularly in women damaged due to insufficient support, normally provided by dehydroepiandrosterone and its intracrine processing. Dehydroepiandrosterone deficiency at the time of adrenopause seems to us as the more likely endocrine trigger than estrogen deficiency caused by menopause as androgens in general are considered to be protective against autoimmunity and estrogens to favor it. Acinar cell is normally responsible for the production of primary saliva. Acinar cell damage can lead to acinar cell apoptosis and loss. Normally this is compensated by division of the acinar cells in situ or, according to recent reports, perhaps rather by division and subsequent migration of one of the daughter cells into the acinar space and transdifferentiation of this intercalated ductal cell progenitor into mature acinar cell. In ...
Foods good for pancreas : What is Pancreatic Inflammation? Diet suitable for inflammation of the pancreas.Worldwide, more than 10,000..
The stress-activated protein kinase (SAPK) p38 can induce apoptosis, and its inhibition facilitates mammary tumorigenesis. g38 signaling forms regular mammary acinar morphogenesis and inhibits HER2/neu-driven tumorigenesis. Our data also define at what stage of mammary gland advancement g38 might action to suppress tumorigenesis and how its inhibition could speed up disease development. Outcomes Reduction of ECM connection activates MKK6-g38 signaling and anoikis in MCF-10A cells Integrin and development aspect signaling become uncoupled in MCF-10A cells harvested in suspension system, starting a tension indication that outcomes in cell loss of life (19). Consistent with prior research (7), immunoblot (IB) evaluation indicated that g38 phosphorylation was MK-2866 elevated in separate MCF-10A cells essential contraindications to that in attached cells (Fig. 1A). We also noticed account activation of g38 in principal mouse MECs (mMECs) and immortalized mouse embryonic fibroblasts (MEFs) harvested ...
A malignant tumor arising from secreting cells of a racemose gland, particularly the salivary glands. Racemose (Latin racemosus, full of clusters) refers, as does acinar (Latin acinus, grape), to small saclike dilatations in various glands. Acinar cell carcinomas are usually well differentiated and account for about 13% of the cancers arising in the parotid gland. Lymph node metastasis occurs in about 16% of cases. Local recurrences and distant metastases many years after treatment are common. This tumor appears in all age groups and is most common in women. (Stedman, 25th ed; Holland et al., Cancer Medicine, 3d ed, p1240; from DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, p575 ...
The present study demonstrates that p8 is expressed in human islets and certain rodent pancreatic β-cell lines. Furthermore, proliferation and p8 expression in rat INS-1 β-cells is strongly inducible by high glucose concentrations in the medium. Our findings point to a possible new role of p8 as a mediator of glucose-induced pancreatic β-cell proliferation. This hypothesis is supported by studies demonstrating increased cell growth in p8 overexpressing rat AR42J pancreatic acinar cells (6) and human HeLa cervix epithelial cells (7). Moreover, glucose-induced pancreatic β-cell proliferation involves activation of PKC (2,3), and rat as well as human p8 amino acid sequences contain a highly conserved region with two PKC sites (16).. p8 was originally postulated as a pancreatic emergency gene product, which is exclusively upregulated in pancreatic acinar cells during stress caused by acute pancreatitis (6). The emergency gene character was underlined by the strong increase of pancreatic p8 ...
In these experiments, TG was used to deplete Ca2+ stores in cells maintained in nominally Ca2+-free extracellular solution. Addition of Ca2+ to the extracellular solution resulted in SOCE-mediated increase in [Ca2+]c (cytosolic Ca2+ concentration) followed by formation of an elevated [Ca2+]c plateau (Figure 4). Addition of InsP3-producing secretagogue ACh reversibly decreased the amplitude of the plateau (Figure 4A, n=465). Using the Mn quench technique [20] we also observed a small (13±2%) but statistically significant inhibition of the influx by 300 nM ACh (Supplementary Figure S5 available at http://www.BiochemJ.org/bj/436/bj4360231add.htm, n=145 cells in ACh-treated group and n=139 cells in control group). Caffeine (10 mM), which in PACs very efficiently blocks InsP3-induced Ca2+ responses [21] has no effect on its own (Figure 4B, n=169), but it partially reversed the ACh-induced reduction of the plateau (Figure 4C, n=168). These experiments suggest that activation of IP3Rs has a mild ...
Della torre, G, Ultracytochemical characterization of c particle-producing vacuoles in pancreatic acinar cells of the mouse. (1974). Subject Strain Bibliography 1974. 1981 ...
GSK-7975A inhibits CRAC entry (Fura-2 340:380 normalized at 700 s). (A) Changes in mouse pancreatic acinar [Ca2+]C induced by CCK (1 nmol/L) with external physi
the expression of caspase-1 is a frequent event in chronic pancreatitis and its distribution pattern may reflect two functions of this protease: on one hand its participation in the apoptotic pathway in atrophic acinar cells and, on the other hand, its role in proliferation and differentiation in proliferating duct cells. PMID: 11345139 ...
Mouse polyclonal antibody raised against a full-length human ZSCAN23 protein. ZSCAN23 (ABZ92213.1, 1 a.a. ~ 389 a.a) full-length human protein. (H00222696-B01P) - Products - Abnova
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Regulated secretion from pancreatic acinar cells occurs by exocytosis of zymogen granules (ZG) at the apical plasmalemma. ZGs originate from the TGN and undergo prolonged maturation and condensation. After exocytosis, the zymogen granule membrane (ZGM) is retrieved from the plasma membrane and ultimately reaches the TGN. In this study, we analyzed the fate of a low M(r) GTP-binding protein during induced exocytosis and membrane retrieval using immunoblots as well as light and electron microscopic immunocytochemistry. This 27-kD protein, identified by a monoclonal antibody that recognizes rab3A and B, may be a novel rab3 isoform. In resting acinar cells, the rab3-like protein was detected primarily on the cytoplasmic face of ZGs, with little labeling of the Golgi complex and no significant labeling of the apical plasmalemma or any other intracellular membranes. Stimulation of pancreatic lobules in vitro by carbamylcholine for 15 min, resulted in massive exocytosis that led to a near doubling of ...
A promising strategy to fresh diabetes therapies is to generate cells from additional differentiated pancreatic cells reprogramming indicates that acinar cells keep guarantee as a resource for fresh islet cells in regenerative therapies for diabetes. [12C15] offers been reported. In particular, in research on acinar-to-cell transdifferentiation cell-lineage doing a trace for demonstrated that acinar cells lead just to acinar cell regeneration, not really to -cell regeneration, in versions of pancreatitis triggered by incomplete pancreatectomy, cerulein shot, or pancreatic duct ligation [24]. Strobel et al. [25] also utilized hereditary cell-lineage looking up to examine whether ATF1 the transdifferentiation of acinar cells has a function in regeneration and metaplasia in pancreatitis. Their outcomes demonstrated that acinar cells are regenerated just from preexisting acinar cells, and that acinar-to-ductal transdifferentiation takes place in the pancreas of adult rodents, but makes just little ...
Despite expression of oncogenic KRAS, premalignant pancreatic intraepithelial neoplasia 1 (PanIN1) lesions rarely become fully malignant pancreatic ductal adenocarcinoma (PDAC). The molecular mechanisms through which established risk factors, such as chronic pancreatitis, acinar cell damage, and/or defective autophagy increase the likelihood of PDAC development are poorly understood. We show that accumulation of the autophagy substrate p62/SQSTM1 in stressed KrasG12D acinar cells is associated with PDAC development and maintenance of malignancy in human cells and mice. p62 accumulation promotes neoplastic progression by controlling the NRF2-mediated induction of MDM2, which acts through p53-dependent and -independent mechanisms to abrogate checkpoints that prevent conversion of differentiated acinar cells to proliferative ductal progenitors. MDM2 targeting may be useful for preventing PDAC development in high-risk individuals ...
The lacrimal gland (LG) is an exocrine gland important for secretion of the tear film. The kinase p38 has important signal transduction functions, e.g. in gene transcription, but has previously not been known to modulate exocrine secretion. The aim of the current study was to investigate the role of p38 in carbachol (Cch)-induced LG secretion in LG acinar cells in vitro. Western blotting was used to determine the phosphorylation status of p38 and p42/44 and determine expression of p38 isoforms. To determine the effect of p38 inhibition on LG secretion, PD 169316, a general p38 inhibitor, and SB 239063, an inhibitor of p38α and β, were added to the cells prior to secretion measurements. The results revealed activation of p38 mediated by Cch stimulation and inhibition of Cch-induced secretion as a result of p38 inhibition. The inhibition was observed with PD 169316 isoforms, but not with SB 239063. The p38δ isoform was shown to have robust expression both by Western blotting of acinar cells and ...
Results Non-neoplastic acinar cells were stained diffusely, but epithelial cells of the pancreatic duct and the islets of Langerhans were not stained. In pancreatic tumours, all the seven ACCs were diffusely positive for the 2P-1-2-1 antibody. However, no positive staining was found in other pancreatic tumours including NETs, SPNs and ductal adenocarcinomas. The sensitivity and specificity of the 2P-1-2-1 antibody for ACCs were both 100%. In other organs studied, positive staining was observed only in the ectopic pancreas. ...
Acute pancreatitis (AP), a human disease in which the pancreas digests itself, has substantial mortality with no specific therapy. The major causes of AP are alcohol abuse and gallstone complications, but it also occurs as an important side effect of the standard asparaginase-based therapy for childhood acute lymphoblastic leukemia. Previous investigations into the mechanisms underlying pancreatic acinar cell death induced by alcohol metabolites, bile acids, or asparaginase indicated that loss of intracellular ATP generation is an important factor. We now report that, in isolated mouse pancreatic acinar cells or cell clusters, removal of extracellular glucose had little effect on this ATP loss, suggesting that glucose metabolism was severely inhibited under these conditions. Surprisingly, we show that replacing glucose with galactose prevented or markedly reduced the loss of ATP and any subsequent necrosis. Addition of pyruvate had a similar protective effect. We also studied the effect of ...
The splicing-factor oncoprotein SRSF1 (also known as SF2/ASF or ASF/SF2) is upregulated in breast cancers. We investigated the ability of SRSF1 to transform human and mouse mammary epithelial cells in vivo and in vitro. SRSF1-overexpressing COMMA-1D cells formed tumors, following orthotopic transplantation to reconstitute the mammary gland. In three-dimensional (3D) culture, SRSF1-overexpressing MCF-10A cells formed larger acini than control cells, reflecting increased proliferation and delayed apoptosis during acinar morphogenesis. These effects required the first RNA-recognition motif and nuclear functions of SRSF1. SRSF1 overexpression promoted alternative splicing of BIM (also known as BCL2L11) and BIN1 to produce isoforms that lack pro-apoptotic functions and contribute to the phenotype. Finally, SRSF1 cooperated specifically with MYC to transform mammary epithelial cells, in part by potentiating eIF4E activation, and these cooperating oncogenes are significantly coexpressed in human breast ...
Pancreatic ductal adenocarcinoma (PDAC) is the third leading cause of cancer death in North America. The highest risk factor for PDAC is recurrent pancreatitis. While the link between PDAC and pancreatitis is unknown, de-differentiation of acinar cells is common to both diseases. Our lab has shown that Activating Transcription Factor 3 (ATF3), a factor upregulated during pancreatic injury, contributes to the development of acinar-to-ductal cell metaplasia (ADM), a precursor phenotype of PDAC. The goal of this study was to identify how ATF3 contributes to ADM. I hypothesize that ATF3 regulates acinar gene expression promoting ADM. We observed decreased ADM development in Atf3-/- acinar cultures, along with expression changes in differentiation genes and ADM promoting pathways (EGFR) in vivo. Assessment following chronic injury indicated absence of ATF3 resulted in decreased tissue damage. These results suggest a novel mechanism where ATF3 promotes ADM through loss of the mature acinar cell phenotype.
The role of tuberous sclerosis complex (TSC) in the pathogenesis of pancreatic cancers remains largely unknown. The present study shows that neurogenin 3 directed Cre deletion of Tsc1 gene induces the development of pancreatic acinar carcinoma. By cross-breeding the Neurog3-cre mice with Tsc1(loxp/loxp) mice, we generated the Neurog3-Tsc1-/- transgenic mice in which Tsc1 gene is deleted and mTOR signaling activated in the pancreatic progenitor cells. All Neurog3-Tsc1-/- mice developed notable adenocarcinoma-like lesions in pancreas starting from the age of 100 days old. The tumor lesions are composed of cells with morphological and molecular resemblance to acinar cells. Metastasis of neoplasm to liver and lung was detected in 5% of animals. Inhibition of mTOR signaling by rapamycin significantly attenuated the growth of the neoplasm. Relapse of the neoplasm occurred within 14 days upon cessation of rapamycin treatment. Our studies indicate that activation of mTOR signaling in the pancreatic ...
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Secretory granules contain specific proteins and other macromolecules that are destined for secretion into the extracellular space. This slide shows secretory granules in pancreatic acinar cells. Their size is approximately 1 µm in diameter, and they accumulate on the apical side of the cell above the nucleus. ...
TY - GEN. T1 - Role of Vascular Endothelial Growth Factor-D (VEGF-D) on IL-6 expression in cerulein-stimulated pancreatic acinar cells. AU - Lee, Jangwon. AU - Kyung, Hwan Kim. AU - Kim, Hyeyoung. PY - 2007/1. Y1 - 2007/1. N2 - Cerulein pancreatitis is similar to human edematous pancreatitis with dysregulation of the digestive enzyme production and cytoplasmic vacuolization, the death of acinar cells, edema formation, and an infiltration of inflammatory cells into the pancreas. Our preliminary proteomic analysis showed increased expression of vascular endothelial growth factor-D (VEGF-D) in cerulein (10 -8M)-treated pancreatic acinar cells. We hypothesized that VEGF-D may have a role in cerulein-induced cytokine expression in pancreatic acinar cells. This article was designed to elucidate whether cerulein induces VEGF-D expression in pancreatic AR42J cells, and whether VEGF-D expression is related to interleukin-6 (IL-6) expression by transfecting antisense oligonucleotide (AS ODN) for VEGF-D ...
TY - JOUR. T1 - Identification of a novel aquaporin, AQP12, expressed in pancreatic acinar cells. AU - Itoh, Tomohiro. AU - Rai, Tatemitsu. AU - Kuwahara, Michio. AU - Ko, Shigeru B.H.. AU - Uchida, Shinichi. AU - Sasaki, Sei. AU - Ishibashi, Kenichi. N1 - Funding Information: We thank N. Ozaki (Nagoya university, Japan) for his skillful technical assistance in isolation of pancreatic islet. This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan.. PY - 2005/5/13. Y1 - 2005/5/13. N2 - Members of the aquaporin (AQP) water channel family are widely distributed in various tissues and contribute to the water permeability of epithelial and endothelial cells. Currently 11 members of the AQP family (AQP0-10) have been reported in mammals. Here we report the identification of AQP12, which we found by performing a BLAST program search. Northern blot analysis revealed that AQP12 was specifically expressed in the pancreas. Further analysis by in ...
The molecular identities of the pathways that mediate K+ and Cl− effluxes in serous acinar cells are unknown. CFTR mRNA is expressed in these cells, and immunocytochemistry revealed it to be present near the apical membrane. To examine the role of CFTR in Ca2+-evoked fluid secretion, we employed cells from the cftrtm1Unc−/− mouse and we pharmacologically inhibited CFTR in WT mouse cells. The results demonstrated that mouse nasal acinar cells from both WT and cftrtm1Unc−/− mice, as well as WT cells treated with CFTRinh172, all exhibited similar rates of Cl− efflux in response to muscarinic stimulation. Isolated intact submucosal glands from CF individuals that lack functional CFTR can secrete fluid in response to cholinergic agonists (Joo et al. 2002a), and it was recently shown that intact glands in WT and cftrtm1Unc−/− mice trachea secrete fluid in response to CCh at similar rates (Ianowski et al. 2007). It has remained unclear if cholinergic-induced fluid secretion in CF glands ...
The inability to visualize the initiation and progression of type-1 diabetes (T1D) noninvasively in humans is a major research and clinical stumbling block. We describe an advanced, exportable method for imaging the pancreatic inflammation underlying T1D, based on MRI of the clinically approved magnetic nanoparticle (MNP) ferumoxytol. The MNP-MRI approach, which reflects nanoparticle uptake by macrophages in the inflamed pancreatic lesion, has been validated extensively in mouse models of T1D and in a pilot human study. The methodological advances reported here were enabled by extensive optimization of image acquisition at 3T, as well as by the development of improved MRI registration and visualization technologies. A proof-of-principle study on patients recently diagnosed with T1D versus healthy controls yielded two major findings: First, there was a clear difference in whole-pancreas nanoparticle accumulation in patients and controls; second, the patients with T1D exhibited pronounced inter- ...
Morphological analysis of transplantable pancreatic acinar carcinoma of rat has revealed a continuum of cells from those which totally lack mature secretory granules to cells with abundant well-formed secretory granules. This tumor is easily dissociable into single cells which incorporate [3H]thymidine and [3H]leucine into DNA and protein at a linear rate for 3 to 4 hr. The number of cells synthesizing DNA, as assessed by light microscope autoradiography, was about 17%. Dissociated neoplastic pancreatic acinar cells are classified into four cell types (types I to IV) on the basis of nuclear morphology, degree of polarization, and the extent of specialization of cytoplasmic organelles. Type IV cells possess all the characteristics of mature pancreatic acinar cells, such as abundant rough endoplasmic reticulum, prominent Golgi apparatus, and numerous secretory granules. Cell types III, II, and I are progressively less differentiated. An analysis of the cytological features of 285 dissociated tumor ...
PI3K/Akt activation plays a critical role in the regeneration of pancreatic acini after resection. Furthermore, pancreatic regeneration is markedly attenuated in the aged pancreas most likely because of decreased PI3K/Akt activation.
The human body is in a constant race to replace cells, tissues, and whole organs that are lost due to injury, disease, and normal aging. Although the body has remarkable inherent regenerative capacity, it often cannot keep pace. Donor cells and tissues are available in limited quantities and are beset by problems such as immune compatibility and transplant rejection. Recently, technologies to convert one cell type into another, so-called direct reprogramming, have provided promise as a new source of cells for regenerative medicine. Li et al. now provide an enhanced method to directly reprogram native mouse pancreatic acinar cells into induced pancreatic beta cells that are functional and persist long-term.. A wave of recent studies have shown that cell identity can be readily modulated by the expression of key transcription factors. However, there remain significant uncertainties about whether the cells created by direct reprogramming will prove useful in regenerative medicine, as their ...
Adventitia and perivascular adipose tissue the integral unit in vascular disease (Chapter 20).. New! Arginase-II promotes tumor necrosis factor-α release from pancreatic acinar cells causing β-cell apoptosis in aging. ...
J:102698 Murtaugh LC, Law AC, Dor Y, Melton DA, Beta-catenin is essential for pancreatic acinar but not islet development. Development. 2005 Nov;132(21):4663-74 ...
Acinar cells make up 82% of the total pancreas; these cells are responsible for the production of the digestive enzymes. ... "Acinar Cell". Auckland Bioengineering Institute-University of Auckland. Archived from the original on 26 February 2008. ... Pancreatitis can result in exocrine pancreatic insufficiency, if the organ's acinar cells are permanently damaged; the ... either continued to exhibit inflammation of the organ or had decreased acinar cell function, though they had no pancreatitis ...
September 2005). "Acinar cell apoptosis in Serpini2-deficient mice models pancreatic insufficiency". PLOS Genetics. 1 (3): e38 ... Cell. 130 (6): 1108-1119. doi:10.1016/j.cell.2007.07.013. PMC 2128786. PMID 17889653. Hejgaard J, Rasmussen SK, Brandt A, ... "Squamous cell carcinoma antigen 2 is a novel serpin that inhibits the chymotrypsin-like proteinases cathepsin G and mast cell ... Within the cell, serpin polymers are slowly removed via degradation in the endoplasmic reticulum. However, the details of how ...
VAMP8 also participates the regulated exocytosis in pancreatic acinar cells. VAMP4 is involved in transport from the Golgi. ... Cell. 10 (6): 1957-72. doi:10.1091/mbc.10.6.1957. PMC 25394. PMID 10359608. Vesicle-Associated+Membrane+Protein+1 at the US ...
... "cAMP potentiates ATP-evoked calcium signaling in human parotid acinar cells". The Journal of Biological Chemistry. 279 (38): ... and in fat cells. P2X4 receptors have been implicated in the regulation of cardiac function, ATP-mediated cell death, synaptic ... Continued binding leads to increased permeability to N-methyl-D-glucamine (NMDG+) in about 50% of the cells expressing the P2X4 ... The P2X4 receptor has high calcium permeability, leading to the depolarization of the cell membrane and the activation of ...
"Ca2+ signalling and Ca2+-activated ion channels in exocrine acinar cells". Cell Calcium. 38 (3-4): 171-200. doi:10.1016/j.ceca. ... Signaling occurs when the cell is stimulated to release Ca2+ ions from intracellular stores, and/or when Ca2+ enters the cell ... Nanodomain European Calcium Society Clapham DE (December 2007). "Calcium signaling". Cell. 131 (6): 1047-58. doi:10.1016/j.cell ... Many cell surface receptors, including G protein-coupled receptors and receptor tyrosine kinases, activate the PLC enzyme. PLC ...
When monkey lacrimal acinar cells are stressed with inflammatory cytokines (as occurs in dry eye), carbachol loses its capacity ... Cell surface proteoglycan syndecan-1 is partly responsible. Biotinylated cell surface proteins from a lacritin-responsive cell ... However, in secretion assays using monkey lacritin on monkey lacrimal acinar cells, the dose response appears to be sigmoidal ... In lacrimal gland, polarized lacrimal acinar cells appear to be the most prolific lacritin producers, as evidenced by strong ...
Parasympathetic stimulation leads to acetylcholine (ACh) release onto the salivary acinar cells. ACh binds to muscarinic ... In humans, saliva is around 99% water, plus electrolytes, mucus, white blood cells, epithelial cells (from which DNA can be ... secreted by the acinar cells of the parotid and submandibular glands, starts the digestion of starch before the food is even ... which is secreted by the acinar cells of the sublingual gland; has a pH optimum around 4.0 so it is not activated until ...
Dynamics of cytoplasmic membranes in pancreatic acinar cells". Philosophical Transactions of the Royal Society of London. B, ... The cells of the superficial cell layer that lines the lumen are known as facet cells or umbrella cells. This layer is the only ... Cells of the basal layer are cuboidal (cube-shaped), or columnar (column-shaped), while the cells of the superficial layer vary ... The intermediate cell layer is highly proliferative and, therefore, provides for rapid cell regeneration in response to injury ...
5-trisphosphate receptors in parotid acinar cells. A mechanism for the synergistic effects of cAMP on Ca2+ signaling". J. Biol ... induced apoptosis of human T-lymphoma cells". Cell Calcium. 27 (6): 315-28. doi:10.1054/ceca.2000.0126. PMID 11013462. Mayne M ... Cell. Signal. 20 (4): 737-47. doi:10.1016/j.cellsig.2007.12.010. PMID 18249094. Wilker E, Mittleman MA, Litonjua AA, et al. ( ... Cell Biol. 183 (2): 297-311. doi:10.1083/jcb.200803172. PMC 2568025. PMID 18936250. Johnson JM, Castle J, Garrett-Engele P, et ...
"Subcellular distribution and function of Rab3A-D in pancreatic acinar AR42J cells". Biochem. Biophys. Res. Commun. 287 (3): 746 ... Weber E, Jilling T, Kirk KL (March 1996). "Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells". J. ... Cell. 96 (3): 363-74. doi:10.1016/S0092-8674(00)80549-8. PMID 10025402. S2CID 15162326. Jung YJ, Lee TH, Lee JY, Kim JH, Park ... Mol Cell Biol. 15 (3): 1137-43. doi:10.1128/MCB.15.3.1137. PMC 230335. PMID 7532276. "Entrez Gene: RAB3A RAB3A, member RAS ...
"Pancreatic ductal and acinar cell neoplasms in Carney complex: a possible new association". J Clin Endocrinol Metab. 96 (11): ... Testicular cancer, particularly Sertoli cell type, is associated with Carney syndrome. Thyroid and pancreas cancer may also ...
... other cell types have followed suit, as exemplified by the pancreas (acinar and beta cells), T-cells, and smooth muscle. Levels ... "Cell-permeant NAADP: a novel chemical tool enabling the study of Ca2+ signalling in intact cells". Cell Calcium. 43 (6): 531-8 ... A 2'-3'-phosphatase stimulated by Ca2+ has been proposed in brain and, possibly in pancreatic acinar cells, that catabolises ... For example, NAADP/TPCs are unique drivers of cell killing by Cytotoxic T cells. Similarly, phagocytosis via the Fc receptor in ...
They co-localize with Orai channels (TC# 1.A.52) in pancreatic acinar cells. IP3 receptors possess three domains: N-terminal ... "InsP₃receptors and Orai channels in pancreatic acinar cells: co-localization and its consequences". The Biochemical Journal. ... They predominate in the endoplasmic reticular membranes of various cell types in the brain but have also been found in the ... Ry receptors occur primarily in muscle cell sarcoplasmic reticular (SR) membranes, and IP3 receptors occur primarily in brain ...
"Rab4 associates with the actin terminal web in developing rat pancreatic acinar cells". European Journal of Cell Biology. 72 (1 ... Molecular Biology of the Cell. 11 (7): 2201-11. doi:10.1091/mbc.11.7.2201. PMC 14913. PMID 10888662. Li L, Omata W, Kojima I, ... dephosphorylation determines the localization of rab4 during the cell cycle". The EMBO Journal. 11 (12): 4379-89. doi:10.1002/j ...
"Fibronectin expression modulates mammary epithelial cell proliferation during acinar differentiation". Cancer Research. 68 (9 ... The RGD sequence (Arg-Gly-Asp) is located in III10 and is the site of cell attachment via α5β1 and αVβ3 integrins on the cell ... ISBN 978-0-387-97050-9. Han S, Khuri FR, Roman J (January 2006). "Fibronectin stimulates non-small cell lung carcinoma cell ... in human dermal blood endothelial cells. As a consequence, knockdown of CD93 in these cells resulted in the disruption of the ...
2003). "CRHSP-24 phosphorylation is regulated by multiple signaling pathways in pancreatic acinar cells". Am. J. Physiol. ... doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. v t e (Articles with short description, Short description matches ... Kim JE, Tannenbaum SR, White FM (2005). "Global phosphoproteome of HT-29 human colon adenocarcinoma cells". J. Proteome Res. 4 ... 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635-48. ...
In addition to promoting the inflammatory cell reaction to acinar cells, ceruletide induces pancreatitis through dysregulation ... "Cerulein upregulates ICAM-1 in pancreatic acinar cells, which mediates neutrophil adhesion to these cells". American Journal of ... Ceruletide upregulates pancreatic acinar cell intercellular adhesion molecule-1 (ICAM-1) proteins through intracellular ... Surface ICAM-1 in turn promotes neutrophil adhesion onto acinar cells enhancing pancreatic inflammation. ...
"Expression and localization of rab escort protein isoforms in parotid acinar cells from rat". Journal of Cellular Physiology. ... June 2004). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation ...
June 2002). "Loss of hyperpolarization-activated Cl(-) current in salivary acinar cells from Clcn2 knockout mice". The Journal ... Cell Physiology. 282 (4): C805-16. doi:10.1152/ajpcell.00291.2001. PMID 11880269. Nehrke K, Arreola J, Nguyen HV, Pilato J, ... Cell Physiology. 290 (1): C45-56. CiteSeerX 10.1.1.533.5601. doi:10.1152/ajpcell.00209.2005. PMID 16049054. S2CID 8655480. ... "Expression of voltage-gated chloride channels in human glioma cells". The Journal of Neuroscience. 23 (13): 5572-82. doi: ...
2003). "CRHSP-24 phosphorylation is regulated by multiple signaling pathways in pancreatic acinar cells". Am. J. Physiol. ... J Cell Mol Med. 7 (1): 35-42. doi:10.1111/j.1582-4934.2003.tb00200.x. PMC 6740078. PMID 12767259. "Entrez Gene: CSDC2 cold ...
ATPases in rat submandibular acinar cells". Cell Calcium. 43 (5): 469-481. doi:10.1016/j.ceca.2007.08.001. PMID 17889347. ... The number of mitochondria in a cell can vary widely by organism, tissue, and cell type. A mature red blood cell has no ... The bottleneck exploits stochastic processes in the cell to increase the cell-to-cell variability in mutant load as an organism ... and cell death, as well as maintaining control of the cell cycle and cell growth. Mitochondrial biogenesis is in turn ...
... is also present in secondary granules of PMNs and is secreted by some acinar cells. Lactoferrin can be purified ... Many viruses tend to bind to the lipoproteins of the cell membranes and then penetrate into the cell. Lactoferrin binds to the ... Such an indirect antiviral effect is achieved by affecting natural killer cells, granulocytes and macrophages - cells, which ... Lactoferrin is one of the transferrin proteins that transfer iron to the cells and control the level of free iron in the blood ...
... a GPI-anchored protein in the apical plasma membrane of the pancreatic acinar cell, co-immunoprecipitates with src kinases and ... "Nonparallel secretion of GP-2 from exocrine pancreas implies luminal coupling between acinar and duct cells". Am. J. Physiol. ... Cell Biol. 70 (10-11): 1105-14. doi:10.1139/o92-156. PMID 1284286. Fukuoka S, Freedman SD, Yu H, et al. (1992). "GP-2/THP gene ... J. Cell Biol. 53 (1): 154-63. PMID 2076702. Hoops TC, Ivanov I, Cui Z, et al. (1994). "Incorporation of the pancreatic membrane ...
In the pancreatic acinar cells, UBR1 is more highly expressed than anywhere else in the body. Impairment of the ubiquitin- ... necessary for cellular processes such as cell division, cell signaling, cell surface receptor function, apoptosis, DNA ... Baker RT, Varshavsky A (February 1991). "Inhibition of the N-end rule pathway in living cells". Proc Natl Acad Sci USA. 88 (4 ... Varshavsky A (January 1997). "The N-end rule pathway of protein degradation". Genes Cells. 2 (1): 13-28. doi:10.1046/j.1365- ...
Imai A, Nashida T, Yoshie S, Shimomura H (Aug 2003). "Intracellular localisation of SNARE proteins in rat parotid acinar cells ... Imai A, Nashida T, Yoshie S, Shimomura H (2003). "Intracellular localisation of SNARE proteins in rat parotid acinar cells: ... "Interaction of Munc-18-2 with syntaxin 3 controls the association of apical SNAREs in epithelial cells". J. Cell Sci. 111 (17 ... "Human syntaxin 3 is localized apically in human intestinal cells". J. Cell Sci. 110 (18): 2207-14. doi:10.1242/jcs.110.18.2207 ...
Imai A, Nashida T, Yoshie S, Shimomura H (Aug 2003). "Intracellular localisation of SNARE proteins in rat parotid acinar cells ... Journal of Cell Science. 112. 112 (6): 845-54. doi:10.1242/jcs.112.6.845. PMID 10036234. Margittai M, Otto H, Jahn R (Mar 1999 ... Cell. 83 (1): 111-9. doi:10.1016/0092-8674(95)90239-2. PMID 7553862. S2CID 675343. Pérez-Brangulí F, Muhaisen A, Blasi J (Jun ... The Journal of Cell Biology. 143 (4): 957-71. doi:10.1083/jcb.143.4.957. PMC 2132958. PMID 9817754. Nishimura Y, Hayashi M, ...
Imai A, Nashida T, Yoshie S, Shimomura H (Aug 2003). "Intracellular localisation of SNARE proteins in rat parotid acinar cells ... "Interaction of Munc-18-2 with syntaxin 3 controls the association of apical SNAREs in epithelial cells". Journal of Cell ... "Relocation of the t-SNARE SNAP-23 from lamellipodia-like cell surface projections regulates compound exocytosis in mast cells ... Synaptobrevin/VAMP and syntaxin are believed to be involved in vesicular transport in most, if not all cells, while SNAP25 is ...
Excess hyaline basement membrane material is deposited between cells, and there is also acinar atrophy and destruction.[ ... that are mainly composed of ductal cells with occasional myoepithelial cells. ...
Imai A, Nashida T, Yoshie S, Shimomura H (Aug 2003). "Intracellular localisation of SNARE proteins in rat parotid acinar cells ... Journal of Cell Science. 115 (Pt 6): 1321-30. doi:10.1242/jcs.115.6.1321. PMID 11884531. Polgár J, Chung SH, Reed GL (Aug 2002 ... Cell. 74 (5): 863-73. doi:10.1016/0092-8674(93)90466-4. PMID 7690687. S2CID 37698687. Li C, Ullrich B, Zhang JZ, Anderson RG, ... Molecular Cell. 3 (6): 751-60. doi:10.1016/S1097-2765(01)80007-1. PMID 10394363. Thomas DM, Ferguson GD, Herschman HR, Elferink ...
"The bHLH transcription factor Mist1 is required to maintain exocrine pancreas cell organization and acinar cell identity". J. ... Cell Genet. 86 (3-4): 219-22. doi:10.1159/000015342. PMID 10575209. S2CID 42840963. Pin CL, Rukstalis JM, Johnson C, Konieczny ... 2006). "Identification of a basic helix-loop-helix transcription factor expressed in mammary gland alveolar cells and required ... Cell Biol. 155 (4): 519-30. doi:10.1083/jcb.200105060. PMC 2198859. PMID 11696558. McLellan AS, Langlands K, Kealey T (2003). " ...
Leydig cell tumors of the testis and pancreatic acinar cell tumors and dietary PFOA consumption.[40] The C8 Science Panel ... between elevated C8 exposure and liver adenomas or pancreatic acinar cell tumors; however, a probable link[vague] was found ... cell infiltration of the placenta to facilitate trophoblastic integration with placental blood supply.[45] Another mechanism ... from the pituitary gland which directly results in Leydig Cell tumorgenesis.[42] A mechanism has not yet been proposed to ...
"Mutant KRas-Induced Mitochondrial Oxidative Stress in Acinar Cells Upregulates EGFR Signaling to Drive Formation of Pancreatic ... not only cancer cells). Thus, drugs that knock out these oncogenes (and thereby kill cancer cells) may also damage normal cells ... "Cell. 128 (4): 683-92. doi:10.1016/j.cell.2007.01.029. PMC 3894624. PMID 17320506.. ... "Cell. 149 (5): 979-93. doi:10.1016/j.cell.2012.04.024. PMC 3414841. PMID 22608084.. ...
Animal studies show that exposure to persin leads to apoptosis in certain types of breast cancer cells. It has also been shown ... leaves which causes necrosis of the acinar epithelium of the lactating mammary gland and the myocardium". Nat. Toxins. 3 (5): ... induces Bim-dependent apoptosis in human breast cancer cells". Mol Cancer Ther. 5 (9): 2300-9. doi:10.1158/1535-7163.MCT-06- ... "Synergistic cytotoxicity between tamoxifen and the plant toxin persin in human breast cancer cells is dependent on Bim ...
... or usual acinar adenocarcinoma. Alkaline phosphatase is more elevated in metastatic than non-metastatic cells. High levels of ... prostate cancer cell population harbors self-renewing long-term tumor-propagating cells that resist castration". Cell Stem Cell ... LNCaP cells express AR, but PC-3 and DU-145 cells express very little or no AR. The proliferation of LNCaP cells is androgen- ... Prostate cancer cells are generally devoid of zinc. Prostate cancer cells save energy by not making citrate, and use the ...
PTPkappa interacts by co-immunoprecipitation with E-cadherin, α-catenin and β-catenin in pancreatic acinar cells prior to the ... PTPkappa mediates homophilic cell-cell aggregation via its extracellular domain. PTPkappa only mediates binding between cells ... In these cells, adhesion to matrigel, transwell migration, and cell growth were all increased following the reduction of PTPRK ... Expression of wild-type PTPkappa in U87-MG and U251-MG cells resulted in a reduction in cell proliferation, migration and ...
... atypical squamous cells of undetermined significance) on the Pap test. On a subsequent biopsy, given the diagnosis of ASAP, the ... In urologic pathology, atypical small acinar proliferation, is a collection of small prostatic glands, on prostate biopsy, ... Bostwick DG, Meiers I (July 2006). "Atypical small acinar proliferation in the prostate: clinical significance in 2006". Arch. ... Sensitivity Flury SC, Galgano MT, Mills SE, Smolkin ME, Theodorescu D (January 2007). "Atypical small acinar proliferation: ...
1999). "Bone morphogenetic protein-6 is a marker of serous acinar cell differentiation in normal and neoplastic human salivary ... antagonizes bone morphogenetic protein signaling and endothelial cell differentiation". Mol. Cell. Biol. 23 (16): 5664-79. doi: ... BMP6 is able to induce all osteogenic markers in mesenchymal stem cells. The bone morphogenetic proteins (BMPs) are a family of ... 1994). "Recombinant Vgr-1/BMP-6-expressing tumors induce fibrosis and endochondral bone formation in vivo". J. Cell Biol. 126 ( ...
... is a form of elastase that is produced in the acinar cells of the pancreas, initially produced as an ... In all these tissues, the presence of inflammatory cells is physiologic and elafin expression is believed to protect against ...
... cpn60 and hsp70 in distinct compartments along the secretory pathway of the acinar cells". J. Cell Sci. 107 (3): 539-49. doi: ... Infection and disease are extremely stressful on the cell. When a cell is under stress, it naturally increases the production ... HSP60 has been shown to be released from specific cells like peripheral blood mononuclear cells (PBMCs) when there are ... March 2007). "Heat shock protein 60: regulatory role on innate immune cells". Cell. Mol. Life Sci. 64 (6): 742-51. doi:10.1007/ ...
It has been observed in various cell types including islet cells. The pyruvate-malate cycle was described in liver and kidney ... glucose and acinar origin of hepatocytes". The Biochemical Journal. 289 (Pt 1): 255-62. doi:10.1042/bj2890255. PMC 1132158. ... Molecular and metabolic mechanisms of insulin resistance and β-cell failure in type 2 diabetes". Nature Reviews Molecular Cell ... May 2008). "Chronic Suppression of Acetyl-CoA Carboxylase 1 in β-Cells Impairs Insulin Secretion via Inhibition of Glucose ...
REGULATION OF FLUID AND ELECTROLYTE SECRETION IN SALIVARY GLAND ACINAR CELLS. Annual Review of Physiology. 2005 Mar 17;67(1): ...
It is synthesized in the acinar cells of the pancreas and stored inside membrane-bounded granules at the apex of the acinar ... Release of the granules from the cell is stimulated by either a hormonal signal or a nerve impulse, and the granules spill into ...
Serum amylase may be normal (in 10% of cases) for cases of acute or chronic pancreatitis (depleted acinar cell mass) and ... Necrotic fat cells appear as shadows, contours of cells, lacking the nucleus, pink, finely granular cytoplasm. It is possible ... 55 years white blood cell count > 16000 cells/mm3 blood glucose > 11.1 mmol/L (> 200 mg/dL) serum AST > 250 IU/L serum LDH > ... The two types of acute pancreatitis are mild and severe, which are defined based on whether the predominant response to cell ...
... acinar cell MeSH C04.557.470.200.025.220 - carcinoma, adenoid cystic MeSH C04.557.470.200.025.232 - carcinoma, ductal MeSH ... b-cell, acute MeSH C04.557.337.428.500.125 - leukemia, B-Cell, chronic MeSH C04.557.337.428.500.500 - leukemia, pre-b-cell MeSH ... T-Cell, chronic MeSH C04.557.337.428.580 - leukemia, t-cell MeSH C04.557.337.428.580.100 - leukemia, t-cell, acute MeSH C04.557 ... T-Cell, chronic MeSH C04.557.337.428.580.400 - leukemia, t-cell, htlv-ii-associated MeSH C04.557.337.440 - leukemia, mast-cell ...
EPI is caused by a progressive loss of the pancreatic cells that make digestive enzymes; loss of digestive enzymes leads to ... In dogs, the most common cause is pancreatic acinar atrophy, arising as a result of genetic conditions, a blocked pancreatic ... the usual cause is by pancreatic acinar atrophy, arising as a result of genetic conditions, a blocked pancreatic duct, or prior ... "Linkage analysis and gene expression profile of pancreatic acinar atrophy in the German Shepherd Dog". Mamm Genome. 16 (12): ...
The PSP is mostly produced in Human by the acinar cells of the pancreas and is secreted in the duodenum by the same pathway ... "Opposite effects of beta-cell differentiation and growth on reg expression in human fetal pancreatic cells". Diabetes. 43 (9): ... The PSP has been shown to be associated with islet cell regeneration and diabetogenesis and may be involved in pancreatic ... Pancreatic Stone Protein (PSP), also known as Lithostathine-1-alpha islet cells regeneration factor (ICRF) or islet of ...
"Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate". Nature ... Cell. 33 (3): 915-924. doi:10.1016/0092-8674(83)90034-X. PMID 6191871. S2CID 21490380. Streb, H.; Irvine, R. F.; Berridge, M. J ... Michael Berridge and colleagues publish their discovery that inositol trisphosphate acts as a second messenger system in cell ...
Most of the HCO3− comes from pancreatic acinar cells in the form of NaHCO3 in an aqueous solution. This is the result of the ... Mucus is made by two types of specialized cells termed mucus cells in the stomach and goblet cells in the intestines. Signals ... These cells cause spontaneous cycles of slow wave potentials that can cause action potentials in smooth muscle cells. They are ... The stimulation for these contractions likely originates in modified smooth muscle cells called interstitial cells of Cajal. ...
... also holds promise to serve as a scaffold for plating cells in culture. Human salivary gland ductal and acinar cells ... RT101 cells with perlecan knocked down by antisense did not show tumor formation in this system, however cells expressing the ... MeWo cells are characteristically less invasive than their clonal variant cell line 70W. One lab studied perlecan expression in ... This was first shown in colon cancer cell lines, and subsequently in cell lines of other tissue origins, but in each case ...
Neuroendocrine cells are present in all regions of the human prostate, most notably around the ducts, but also in the acinar ... Prostatic neuroendocrine cells, also known as endocrine-paracrine cells, are part of a larger regulatory cell population ... The open type cells may in addition receive regulatory signals from luminal molecules The developmental origin of these cells ... Neuroendocrine tumor cells express cytokeratins that are typically expressed by luminal secretory type cells, but lack basal ...
Acinar cell carcinoma of the pancreas, also acinar cell carcinoma, is a rare malignant exocrine tumour of the pancreas. It ... Feb 2010). "[A case of acinar cell carcinoma of pancreas, manifested by subcutaneous nodule as initial clinical symptom]". ... Klimstra, DS.; Heffess, CS.; Oertel, JE.; Rosai, J. (Sep 1992). "Acinar cell carcinoma of the pancreas. A clinicopathologic ... Light microscopy of an acinar cell carcinoma biopsy typically shows granular appearance. Immunohistochemistry is usually ...
... Ann N Y Acad Sci. 1988;547:138-49. doi: ...
Project Title: Physiology/cell biology of pancreatic acinar mitochondrial vitamin B1 uptake. Abstract: Vitamin B1 (thiamin) is ... Our focus in the current proposal is on transport of TPP into mitochondria of pancreatic acinar cells with special emphasis on ... Moreover evidence were obtained to show that chronic exposure of pancreatic acinar cells to alcohol and to specific components ... Grant Abstract: Physiology/cell biology of pancreatic acinar mitochondrial vitamin B1 uptake. ...
Cells 8(1), 2018. Calcium mobilizing hormones activate the plasma membrane calcium pump of pancreatic acinar cells. Journal of ... Cell Calcium 11(1): 11-17, 1990. Calcium-stimulated ATPase activity in plasma membrane vesicles from pancreatic acinar cells. ... Calcium efflux across the plasma membrane of rat parotid acinar cells is unaffected by receptor activation or by the microsomal ... Insulin protects pancreatic acinar cells from cytosolic calcium overload and inhibition of plasma membrane calcium pump. ...
Acinar cell adenocarcinoma. The existing literature on treatment of acinar cell adenocarcinoma (ACC) consists largely of ... Systematic Review and Case Series Report of Acinar Cell Carcinoma of the Pancreas. Cancer Control. 2016 Oct. 23 (4):446-454. [ ... Genetic and immunohistochemical analysis of pancreatic acinar cell carcinoma: frequent allelic loss on chromosome 11p and ... Comprehensive genomic profiling of pancreatic acinar cell carcinomas identifies recurrent RAF fusions and frequent inactivation ...
Norman K. Wessells; DNA SYNTHESIS, MITOSIS, AND DIFFERENTIATION IN PANCREATIC ACINAR CELLS IN VITRO . J Cell Biol 1 March 1964 ... DNA SYNTHESIS, MITOSIS, AND DIFFERENTIATION IN PANCREATIC ACINAR CELLS IN VITRO Norman K. Wessells Norman K. Wessells ... Evidence suggests that cells or nuclei which have replicated DNA move inward before dividing. Some daughter cells apparently ... Cells located internally in explants, or in central acini in vivo, show this overt differentiation first. As the epithelia age ...
Acinar cell adenocarcinoma. The existing literature on treatment of acinar cell adenocarcinoma (ACC) consists largely of ... Systematic Review and Case Series Report of Acinar Cell Carcinoma of the Pancreas. Cancer Control. 2016 Oct. 23 (4):446-454. [ ... Genetic and immunohistochemical analysis of pancreatic acinar cell carcinoma: frequent allelic loss on chromosome 11p and ... Comprehensive genomic profiling of pancreatic acinar cell carcinomas identifies recurrent RAF fusions and frequent inactivation ...
Size: 2048*2048 pixels, 16 MoPNG format without background
Exocrine acinar cells. Liver. -. -. -. +. -. Hepatocytes. Spleen. ++. -. +. -. -. Vascular smooth muscle, mononuclear cells. ... Neurons, glial cells, ependymal cells, choroid plexus epithelium. Spinal cord. +. ++. ++. ++. ++. Ependymal cells, neurons, ... Vascular endothelium, mononuclear cells. Esophagus. ++. ++. +. +. -. Epithelium, Vascular smooth muscle, Smooth muscle of ... Numbers of immunohistochemically positive cells: +, few; ++, moderate; +++, numerous; -, virus antigen negative; dpi, days ...
Cystic variant of acinar cell carcinoma of the pancreas presenting as pseudo-pancreatic cyst. Indian Journal of Pathology & ... Cystic variant of acinar cell carcinoma of the pancreas presenting as pseudo-pancreatic cyst. ...
"Acinar Cells" by people in this website by year, and whether "Acinar Cells" was a major or minor topic of these publications. ... "Acinar Cells" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... Below are the most recent publications written about "Acinar Cells" by people in Profiles. ... Below are MeSH descriptors whose meaning is more general than "Acinar Cells". ...
Fatal horse cases-pancreatic lesions (acinar cells), hematopoietic involvement with or without encephalitis. See Reference 27 ... Brain (M)(LV), liver (LV), kidney (LV), marrow (LV) and acinar cells of pancreas ...
We obtained stable MCF10A cells overexpressing wild-type (Wt) Neu/ErbB2 or a constitutively active (CA) variant via retroviral ... However, this signal was restored by salubrinal treatment in Wt-ErbB2 expressing MCF10A cells as these DCIS-like structures ... Wt- and MMTV-Neu cells formed aberrant acini structures resembling DCIS, while CA-ErbB2 overexpression induced invasive lesions ... as well as MMTV-Neu-induced deregulation of acinar growth. This was linked to enhanced CHOP expression, inhibition of ...
acinar cell carcinomas. *cystic tumors. Most pancreatic tumors affect exocrine function.. Endocrine pancreatic cancer. The ... into an artery a substance that stops blood from reaching the cancer cells. Without the blood supply, these cells will die. ... Chemotherapy is a drug treatment that can kill cancer cells and stop them from spreading. A doctor may prescribe it alone or ... Radiation therapy destroys cancer by focusing high energy rays on the cancer cells. It can shrink or remove a tumor. In the ...
... exert the same trophic effect on pancreatic acinar cells indicates that this effect is mediated via high affinity acinar cell ... exert the same trophic effect on pancreatic acinar cells indicates that this effect is mediated via high affinity acinar cell ... exert the same trophic effect on pancreatic acinar cells indicates that this effect is mediated via high affinity acinar cell ... exert the same trophic effect on pancreatic acinar cells indicates that this effect is mediated via high affinity acinar cell ...
We now report that in mouse pancreatic acinar cells, cholecystokinin, but not acetylcholine, evokes rapid and transient ... pharmacological studies alone have implicated these molecules as intracellular second messengers for specific cell surface ... Role of NAADP and cADPR in the induction and maintenance of agonist-evoked Ca2+ spiking in mouse pancreatic acinar cells. ... Role of NAADP and cADPR in the induction and maintenance of agonist-evoked Ca2+ spiking in mouse pancreatic acinar cells. ...
Root bark extracts of Juncus effusus and Paeonia suffruticosa protect salivary gland acinar cells from apoptotic cell death ... Root bark extracts of Juncus effusus and Paeonia suffruticosa protect salivary gland acinar cells from apoptotic cell death ... Root bark extracts of Juncus effusus and Paeonia suffruticosa protect salivary gland acinar cells from apoptotic cell death ... Root bark extracts of Juncus effusus and Paeonia suffruticosa protect salivary gland acinar cells from apoptotic cell death ...
... and cell fate in murine pancreatic acinar cells. American Journal of Physiology - Gastrointestinal and Liver Physiology, 297(6 ... and cell fate in murine pancreatic acinar cells, American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. ... and cell fate in murine pancreatic acinar cells. David N. Criddle, David M. Booth, Rajarshi Mukherjee, Euan McLaughlin, Gary M ... and cell fate in murine isolated pancreatic acinar cells using fluorescence confocal microscopy and patch-clamp ...
Stable acinar progenitor cell model identifies treacle-dependent radioresistance. Authors. Weber TJ; Qian W-J; Smith JN; ...
Itagaki S, Katoh K, Suzuki Y, Nishiyama A. Evidence for increase in K permeability of pancreatic acinar cell during the action ... Evidence for increase in K permeability of pancreatic acinar cell during the action of secretagogues. In: Journal of the ... Evidence for increase in K permeability of pancreatic acinar cell during the action of secretagogues. / Itagaki, S.; Katoh, K ... Itagaki, S, Katoh, K, Suzuki, Y & Nishiyama, A 1982, Evidence for increase in K permeability of pancreatic acinar cell during ...
Whole exome sequencing reveals recurrent mutations in BRCA2 and FAT genes in acinar cell carcinomas of the pancreas」の研究トピックを掘り下 ... Whole exome sequencing reveals recurrent mutations in BRCA2 and FAT genes in acinar cell carcinomas of the pancreas. ...
Acinar Cells / pathology* * Animals * Carcinogenesis* * Carcinoma, Pancreatic Ductal / genetics * Carcinoma, Pancreatic Ductal ... We report that long after its complete resolution, a transient inflammatory event primes pancreatic epithelial cells to ... Such adaptation enables the reactivation of acinar-to-ductal metaplasia (ADM) upon subsequent inflammatory events, thereby ... Upon recovery from acute inflammation, pancreatic epithelial cells display an enduring adaptive response associated with ...
Transmission electron microscope image of mammalian pancreatic acinar cells. Image shows nucleus, mitochondria and rough ...
composed of acinar, ductal and myoepithelial cells. The main lacrimal gland is approximately 15 to 20 mm long, 10 to 12 mm wide ... cells, several layers of wing cells and a single layer of columnar basal cells ... of T and B lymphocytes, macrophages, plasma cells and dendritic cells (Knop and Knop 2005) and serve as an induction site for ... Conjunctival goblet cells are specialized glandular epithelial cells. They are integrated in the conjunctiva epithelium and ...
... where only main cell types (beta cells, duct cells and acinar cells) had been examined for lectin-affinity21. Right here, in ... Hence, correctly purifying acinar cells is an indispensable step towards medical acinar-to-beta cell conversion. Isolation of ... Therefore, great efforts have been made to generate practical beta-cells from non-beta cell sources. Acinar cells are the most ... Earlier studies have got used lectin affinity to acinar cells to purify acinar cells by stream cytometry21,35. These ...
Ca2+ signalling and Ca2+-activated ion channels in exocrine acinar cells. Cell Calcium 38(3-4), pp. 171-200. (10.1016/j.ceca. ... Ca2+ signalling and Ca2+-activated ion channels in exocrine acinar cells. Cell Calcium 38(3-4), pp. 171-200. (10.1016/j.ceca. ... Aberrant Ca2+ signalling through acidic calcium stores in pancreatic acinar cells. Cell Calcium 50(2), pp. 193-199. (10.1016/j. ... Aberrant Ca2+ signalling through acidic calcium stores in pancreatic acinar cells. Cell Calcium 50(2), pp. 193-199. (10.1016/j. ...
The cells retained their epithelial cell morphology and continued to express both ameloblast-specific and ion transport- ... The cells retained their epithelial cell morphology and continued to express both ameloblast-specific and ion transport- ... Methods: To generate 3D HAT-7 structures, cells were dispersed and plated within a Matrigel extracellular matrix scaffold and ... Results: A combination of Hepato-STIM epithelial cell differentiation medium and Matrigel induced the expansion and formation ...
Petersen OH (2005). "Ca2+ signalling and Ca2+-activated ion channels in exocrine acinar cells". Cell Calcium. 38 (3-4): 171-200 ... "Cell. 131 (6): 1047-58. doi:10.1016/j.cell.2007.11.028. PMID 18083096. S2CID 15087548.. ... Signaling occurs when the cell is stimulated to release Ca2+ ions from intracellular stores, and/or when Ca2+ enters the cell ... "Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1863 (9): 2135-46. doi:10.1016/j.bbamcr.2016.05.006. PMID ...
  • Acinar cell carcinoma of the pancreas, also acinar cell carcinoma, is a rare malignant exocrine tumour of the pancreas. (wikipedia.org)
  • IMSEAR at SEARO: Cystic variant of acinar cell carcinoma of the pancreas presenting as pseudo-pancreatic cyst. (who.int)
  • Pancreatic cancer develops when uncontrolled cell growth begins in a part of the pancreas. (medicalnewstoday.com)
  • Acinar cells are the most abundant cell type in the human being and mouse pancreas, a characteristic that makes them an attractive potential source of new beta-cells. (aksumuniversity.org)
  • Stacks of rough endoplasmic reticulum (ER) cisternae seen in a transmission electron micrograph of a section through a secretory acinar cell from the bat exocrine pancreas. (cellimagelibrary.org)
  • Recent findings suggested its origin from pluripotent embryonic stem cells of the pancreas and from embryonic neural precursor cells of the neural crest [6]. (termedia.pl)
  • Cytokeratins and cell differentiation in the pancreas. (humpath.com)
  • The pancreas is made up of 2 types of cells. (epnet.com)
  • They are named for the hormone that is produced by the tumor cells such as gastrinoma (stomach) or insulinoma (pancreas). (epnet.com)
  • Distinct from other epithelia with basal tumours, ΔNp63 + basal cells reportedly do not exist in the normal pancreas. (bmj.com)
  • Results In normal human pancreas, rare ΔNp63 + cells exist in ducts while their prevalence increases in CP and in a subset of PDAC. (bmj.com)
  • In the LPS group, the pancreas was slightly edematous, with the infiltration of a few inflammatory cells and the necrosis of the adjacent fat tissues. (wjgnet.com)
  • Also present in thyroid follicular cells and acinar cells of the pancreas. (powershow.com)
  • The regional distribution and relative frequency of some endocrine cells in the pancreas of the carp, Cyprinus carpio Linnaeus, belonging to the family Cyprinidae in the order Cypriniformes, were observed. (koreamed.org)
  • We re-analyzed multiple pancreas and pancreatic adenocarcinoma single-cell RNA-seq datasets to find that a subset of pancreatic cells, called acinar cells, possess transcriptomes that are very different from an average acinar cell and do not express genes characteristic of acinar cells. (imsc.res.in)
  • Instead, these edge acinar cells, expressed genes characteristic of human embryonic pancreas genes as well as those related to pancreatic cancer progression. (imsc.res.in)
  • These cells thus represent a putative pre-cancerous population in the pancreas. (imsc.res.in)
  • But, so far, there was no hint as to the actual structure within the pancreas upon which the antidiabetic influence of the gland depends and it is primarily to the anatomists, Laguesse and Diamare, that we owe the hypothesis that this must be the collection of cells, named after their discoverer, the isles of Langerhans. (nobelprize.org)
  • A cell present in the acinus of an acinous gland, e.g., of the pancreas. (unboundmedicine.com)
  • The pancreas carries all the SSTR subtypes on the acinar and islet cellular surface ( 17 ). (snmjournals.org)
  • The bHLH transcription factor Mist1 is required to maintain exocrine pancreas cell organization and acinar cell identity. (taconic.com)
  • Our lab has previously shown that direct injection of conditioned media collected from MSC into the pancreas of hyperglycemic mice induced beta cell regeneration and lowered blood glucose levels. (uwo.ca)
  • The pancreas of mice treated with MSC conditioned media had an increased number of beta cells compared to controls, indicating islet regeneration had occurred. (uwo.ca)
  • This causes uncontrolled and catastrophic cell death and the cells burst, releasing their toxic enzymes which digest the pancreas and surrounding tissue. (nutritionreview.org)
  • In a new paper published in the Journal of Biological Chemistry research from Dr Bruce's laboratory shows that insulin, which is normally released from the beta cells of the pancreas, prevents these toxic effects of alcohol and fatty acid metabolites. (nutritionreview.org)
  • These calcium pumps help to restore cellular calcium and prevent the catastrophic cell death and autodigestion of the pancreas. (nutritionreview.org)
  • Pancreatic amylase is secreted by acinar cells of the pancreas and is tissue specific and more temperature labile than salivary amylase. (medscape.com)
  • Blood glucose homeostasis is regulated in the pancreas by clusters of endocrine cells called the islets of Langerhans. (biorxiv.org)
  • The staining pattern of MOv18 in normal tissues was more restricted and was confined to epithelial cells in the lung, kidney, pancreas, salivary gland, ovary, Fallopian tubes, and cervix. (bmj.com)
  • Reactivity was also observed with pneumocytes in the lung, tubuli in the kidney, acinar cells in the salivary gland and pancreas, in the placenta, and with Kupffer cells in the liver. (bmj.com)
  • This track shows data from A Single-Cell Transcriptomic Map of the Human and Mouse Pancreas Reveals Inter- and Intra-cell Population Structure . (ucsc.edu)
  • Pancreas tissue was analyzed using droplet-based single-cell RNA-sequencing (scRNA-seq) and subsequent clustering distinguished 14 pancreas-resident cell types based on their identified marker genes found in Baron et al . (ucsc.edu)
  • There are four bar chart tracks in this track collection with pancreas cells grouped by either batch ( Pancreas Batch ), cell type ( Pancreas Cells ), detailed cell type ( Pancreas Details ) and donor ( Pancreas Donor ). (ucsc.edu)
  • The default track displayed is pancreas cells grouped by cell type. (ucsc.edu)
  • The colors will be purest in the Pancreas Cells subtrack, where the bars represent relatively pure cell types. (ucsc.edu)
  • Our study, insulin+ cells with low levels of PDX1 and MAFA expression, coexpressing MAFB and NPYPYY observed in duct-specific Pdx1-deficient pancreas, strongly recommend that the b-cells formed postnatally remained immature, even at ten weeks of age. (ack1inhibitor.com)
  • Squamous epithelial cells are a specialized cell type found in the epidermis, oropharynx, and additional anatomical locations, but this cell type does not exist in the normal pancreas (Basturk et al. (lxsustentavel.com)
  • In the pancreas, acinar cells secrete a small amount of NaCl-rich fluid, while the duct absorbs the Cl - and secretes HCO 3 - and the bulk of the fluid in the pancreatic juice. (bgu.ac.il)
  • In this review, we focus on the molecular mechanism of fluid and HCO 3 - secretion by the pancreas and salivary glands, to highlight the similarities of the fundamental mechanisms of acinar and duct cell functions, and to point out the differences to meet gland-specific secretions. (bgu.ac.il)
  • Metastatic acinar cell carcinoma of the pancreas. (mayocliniclabs.com)
  • Squamous cell carcinoma with variants of GI tract (stomach small intestine, colon, rectum, pancreas) (closed to accrual 10/17/2018) 7. (ucbraid.org)
  • The two extracts showed a protective effect in NS-SV-Ac cells from the cytotoxicity and apoptosis caused by CDDP. (elsevier.com)
  • Notably, the apoptosis-protective effect of the extracts was not observed in adenocystic carcinoma cell lines. (elsevier.com)
  • [9] [10] High levels of cytoplasmic Ca 2+ can also cause the cell to undergo apoptosis . (wikipedia.org)
  • An increased rate of apoptosis also is seen in conjunctival and lacrimal acinar cells . (medscape.com)
  • Later it became clear that PPARs also play a role in modulating the processes of cell proliferation and differentiation, apoptosis, and aging [ 5 - 8 ]. (hindawi.com)
  • Ginsenoside Rg3 (Rg3) is a trace tetracyclic triterpenoid saponin extracted from ginseng and can induce tumor cell apoptosis, but inhibits tumor cell proliferation, adhesion, invasion and metastasis as well as tumor angiogenesis ( 23 - 29 ). (spandidos-publications.com)
  • Although the pathology is similar for MD and SS, the difference in acinar cell apoptosis and function can explain clinical differences. (elsevier.com)
  • We have compared the effects of these peptides on cytosolic calcium concentration ([Ca 2+ ] c ), mitochondrial metabolism, enzyme secretion, and cell fate in murine isolated pancreatic acinar cells using fluorescence confocal microscopy and patch-clamp electrophysiology. (uthscsa.edu)
  • CCK-58 (1-10 pM) induced transient, oscillatory increases of [Ca 2+ ] c , which showed apical to basolateral progression and were associated with a rise of mitochondrial NAD(P)H. CCK-58 (10 pM) induced zymogen exocytosis in isolated cells and amylase secretion from isolated cells and whole tissues. (uthscsa.edu)
  • [2] These include muscle contraction , neuronal transmission (as in an excitatory synapse ), cellular motility (including the movement of flagella and cilia ), fertilization , cell growth (proliferation), neurogenesis , learning and memory as with synaptic plasticity , and secretion of saliva . (wikipedia.org)
  • 0.01), including missense variants in genes related to pancreatic acinar cells ( GP2 ) and insulin secretion ( GLP1R ). (nature.com)
  • It is now recognized that both major types of DM affects β-cells mass and insulin secretion [ 6 ]. (biomedcentral.com)
  • Epinephrine is a known inhibitor of glucose-stimulated insulin release [1, 9] and it suppressed secretion from the dispersed cells as well as from the intact islets. (researchgate.net)
  • Acinar cells are the site of fluid secretion, and they are responsible for making and secreting more than 85 percent of the proteins involved in producing saliva. (healthywomen.org)
  • Establishing a functional organoid model consisting of myoepithelial and secretory acinar cells is therefore necessary for understanding the coordinated action of these two cell types in unidirectional fluid secretion. (archive.org)
  • HCO3(-) secretion by murine nasal submucosal gland serous acinar cells during Ca2+-stimulated fluid secretion. (upenn.edu)
  • We hypothesize that a predominance of parenchymal fibrosis results in diminished acinar-cell secretion of enzymes. (checkorphan.org)
  • Epithelium can function in: absorption (as in the gastrointestinal tract), secretion (as in a sweat gland), transport and excretion (as in urine formation in the kidney), sensation (taste buds or olfactory cells) as well as for protection. (medicalhistology.us)
  • With time, exocrine cell types expanded while the number of the endocrine cells and their secretion decreased. (elsevier.com)
  • Uraizee I, Hart J, Waxman I, Weber CR, Setia N. Unusual gastric subepithelial tumor: primary mixed acinar-endocrine neoplasm in an anemic woman. (uchicago.edu)
  • Cancer is a genetic disease caused by accumulation of DNA mutations and epigenetic alterations leading to unrestrained cell proliferation and neoplasm formation. (wikipedia.org)
  • Unusual gastric subepithelial tumor: primary mixed acinar-endocrine neoplasm in an anemic woman. (uchicago.edu)
  • A combination of Hepato-STIM epithelial cell differentiation medium and Matrigel induced the expansion and formation of 3D HAT-7 spheroids. (frontiersin.org)
  • The cells retained their epithelial cell morphology and continued to express both ameloblast-specific and ion transport-specific marker genes. (frontiersin.org)
  • RWPE-2 is an epithelial cell isolated from the prostate of a White male patient. (atcc.org)
  • Altered vasculogenic mimicry formation was assessed using immunohistochemistry and PAS staining and associated with the expression of vascular endothelial-cadherin (VE-cadherin), epithelial cell kinase (EphA2), matrix metalloproteinase (MMP)-2 and MMP-9. (spandidos-publications.com)
  • Most recent studies have shown that vascular endothelial-cadherin (VE-cadherin), epithelial cell kinase (EphA2), and matrix metalloproteinase (MMPs) play a crucial role in VM formation ( 13 - 21 ). (spandidos-publications.com)
  • The epithelial cells rest on a condensation of extracellular matrix elaborated both by the epithelial cell as well as underlying connective tissue. (medicalhistology.us)
  • 2007). In later on phases of disease progression, it is known that PDA can transiently shed the manifestation of epithelial cell markers and gain mesenchymal features, in association with metastatic spread (Genovese et al. (lxsustentavel.com)
  • Transmission electron microscope image of mammalian pancreatic acinar cells. (histology-world.com)
  • In individual and mammalian pancreatic acinar cells, cholecystokinin (CCK) and acetylcholine (ACh) are two essential secretagogues that induce particular Ca2+ indicators and stimulate digestive enzyme and liquid release (26). (casein-kinases.com)
  • Alpha cells secrete glucagon and beta cells secrete insulin. (wikibooks.org)
  • Acinar cells will secrete enzymes that will help with digestion. (wikibooks.org)
  • Type II cells, which are smaller and more numerous than Type I cells, secrete pulmonary surfactant. (unboundmedicine.com)
  • Ductal cells cannot secrete fluid. (healthywomen.org)
  • Human bone marrow-derived multipotent stromal cells expanded under Wnt pathway stimulation secrete beta cell-regenerative factors collected as conditioned media (Wnt+ CdM). (uwo.ca)
  • Multipotent stromal cells (MSC), isolated from human bone marrow, secrete a mixture of proteins that can aid in repairing and regenerating damaged cells. (uwo.ca)
  • When alcohol and fat accumulate inside pancreatic acinar cells - the cells that secrete digestive enzymes into the gut - the resulting small molecules called metabolites deplete cellular energy levels and increase cellular calcium. (nutritionreview.org)
  • Over 90% of prostate cancers (PCa) occur in the form of adenocarcinomas, which are characterized by dysregulated growth of the epithelial cells that typically secrete prostate specific antigen (PSA). (oncotarget.com)
  • Acinar cells secrete isotonic fluid in which the major salt is NaCl. (bgu.ac.il)
  • Islets of Langerhans have alpha and beta cells that are responsible for maintaining the glucose concentration in the plasma. (wikibooks.org)
  • By careful studies of the cytological characteristics of the cells of these islets, as distinguished from those of the much more numerous secreting acini among which they lie, and by painstaking examination of the anatomical relationships of the two kinds of cells in different classes of vertebrates, Laguesse and Diamare concluded that the islets must be responsible for the antidiabetic influence. (nobelprize.org)
  • That transition from one steady-state to another was more sluggish in intact islets than in dispersed islet cells is also evident from the experiments with epinephrine. (researchgate.net)
  • However, the return to the basal secretory rate occurred faster in the dispersed cells than in the intact islets. (researchgate.net)
  • The overall dynamics of glucose-induced insulin release was strikingly similar in dispersed cells and intact islets perifused in parallel. (researchgate.net)
  • During the second phase, insulin release from dispersed cells attained a stable plateau rate, whereas the release from intact islets continued to rise. (researchgate.net)
  • Epinephrine (1 muM) inhibited the release in both preparations, but the return to basal rate was faster in the dispersed cells than in the intact islets. (researchgate.net)
  • Conclusion: Acute pancreatitis results in increased tissue reg I protein levels localized to the acinar and ductal cells, and a parallel threefold induction of reg I receptor in the ductal cells, islets, and acinar cells. (elsevier.com)
  • 1. An enteroendocrine cell that produces glucagon and is found in the pancreatic islets. (unboundmedicine.com)
  • Diabetes mellitus is characterized by a loss of insulin-producing beta cells that reside in the pancreatic islets of Langerhans resulting in high blood glucose levels or hyperglycemia. (uwo.ca)
  • Murine islets exhibit a distinct cytoarchitecture consisting of a core of β-cells, surrounded by a mantle of α-, δ-, PP- and ε-cells. (biorxiv.org)
  • Decreased expression of Apigenine b-cell functional genes and improved expression of immature b-cell markers in islets of duct-specific Pdx1-deficient mice. (ack1inhibitor.com)
  • Elevated gene expression of both mafb and LDHA, the latter not expressed in adult b-cells but expressed (in rat islets) up to about 1 week postnatally (39), is consistent with our conclusion on the functional immaturity of those islets. (ack1inhibitor.com)
  • Microscopy showed mild acinar cell damage and greater injury to the capillaries and the islets. (elsevier.com)
  • Freshly isolated islets from a 38-year-old donor were cultured in M3:5 TM medium and placed on a rocker for 14 days to remove contaminated exocrine and mesenchymal cells which attached to the bottom. (elsevier.com)
  • Within seven days of culture, ductular and acinar cells developed within the initially normal islets. (elsevier.com)
  • Role of NAADP and cADPR in the induction and maintenance of agonist-evoked Ca2+ spiking in mouse pancreatic acinar cells. (ox.ac.uk)
  • We now report that in mouse pancreatic acinar cells, cholecystokinin, but not acetylcholine, evokes rapid and transient increases in NAADP levels in a concentration-dependent manner. (ox.ac.uk)
  • Radiation therapy used to treat patients suffering from upper respiratory cancer can also lead to xerophthalmia due to damage to the secretory cells of the lacrimal glands. (christianacare.org)
  • In addition to radiotherapy, this condition can be caused by several autoimmune disorders including Sjogren's syndrome, Steven-Johnson's syndrome and ocular cicatrical pemphigoid, all of which result in damage to the secretory cells of the lacrimal gland and lead to dry eye syndrome (DES). (christianacare.org)
  • While myoepithelial cells have been studied extensively in mammary and lacrimal gland tissues, less is known of the function of myoepithelial cells derived from human salivary glands. (archive.org)
  • An important feature of the disease is infiltration of the salivary and lacrimal (tear) glands by a type of white blood cell known as lymphocytes, which is associated with damage to the glands' secretory function. (lifeextension.com)
  • Lacrimal gland acinar cells in those with MD maintained their function and were not programmed for cell death. (elsevier.com)
  • Exocrine acinar cells and endocrine islet cells are well-differentiated cells which express the keratin combination 8 and 18, whereas the less-differentiated cells of the ductal tree are characterized by the additional expression of keratin 7, keratin 19, and, in the rat, keratin 20. (humpath.com)
  • TOM) mouse model to isolate acinar cells based on reddish fluorescence by circulation cytometry, and have shown the purified acinar cells lack contaminating non-acinar pancreatic cells, such as synaptophysin (SYN)-positive endocrine cells, CD31-positive endothelial cells, Dolichos biflorus agglutinin (DBA)-positive duct cells, vimentin-positive mesenchymal cells and CD45-positive inflammatory cells32,33,34. (aksumuniversity.org)
  • The endocrine, or islet cells, make many hormones. (epnet.com)
  • These data suggest that clustering of endocrine cells during islet morphogenesis is guided, at least in part, by repelling Slit2/3 signals from the pancreatic mesenchyme. (biorxiv.org)
  • At day 60, only a few endocrine cells were identifiable, whereas most of the cells appeared undifferentiated and expressed cytokeratin 7 and 19, neuron specific enolase, tomato lectin, phaseolus leucoagglutinin, laminin, and vimentin. (elsevier.com)
  • This is the first report on the long-term maintenance of human islet cells in culture and allows an insight into the complex process of endocrine cell differentiation. (elsevier.com)
  • The difference is in the parts of the genome that are expressed - a cell's identity is determined by the specific genes that are active within that cell. (hplusmagazine.com)
  • These cells express certain genes specific to their type, and as they move through the process of differentiation, they become increasingly specific types of cells -- ultimately becoming terminally differentiated somatic cells, like astrocytes or red blood cells. (hplusmagazine.com)
  • We set out to investigate whether such transcriptional variation may be large enough such that a small subset of cells may express genes related to cancer inititation before accumulating an oncogenic mutation. (imsc.res.in)
  • Genes within the Idd5 and Idd9/11 diabetes susceptibility loci affect the pathogenic activity of B cells in nonobese diabetic mice. (umassmed.edu)
  • Subcongenic analyses reveal complex interactions between distal chromosome 4 genes controlling diabetogenic B cells and CD4 T cells in nonobese diabetic mice. (umassmed.edu)
  • After filtering out cells with limited numbers of detected genes, the dataset contained 8,629 cells from the four donors. (ucsc.edu)
  • Stable acinar progenitor cell model identifies treacle-dependent radioresistance. (cdc.gov)
  • Objectives/Hypothesis-To create a novel model for development of a tissue-engineered salivary gland from human salivary gland cells that retain progenitor cell markers useful for treatment of radiation-induced xerostomia. (archive.org)
  • Pancreatic acinar cells possess both high and low affinity receptors for cholecystokinin. (umn.edu)
  • The finding that both caerulein and CCK-JMV-180 exert the same trophic effect on pancreatic acinar cells indicates that this effect is mediated via high affinity acinar cell cholecystokinin receptors. (umn.edu)
  • Because secretin and cholecystokinin (CCK) PFTs measure unique aspects of pancreatic function (duct-cell and acinar-cell function, respectively), the use of both hormonal stimulants will allow the most comprehensive investigation of the significance of EUS features. (checkorphan.org)
  • Objective The aggressive basal-like molecular subtype of pancreatic ductal adenocarcinoma (PDAC) harbours a ΔNp63 (p40) gene expression signature reminiscent of a basal cell type. (bmj.com)
  • Pancreatic cancer has four major driver mutations, and as noted in the paper, "The cancer cells used carry two of the three mutations of KRAS , CDKN2A , and TP53 , which are key driver mutations of human [pancreatic ductal adenocarcinoma]. (drugdiscoverynews.com)
  • Rb heterozygous mice develop low-grade C cell adenocarcinoma following biallelic inactivation of the Rb tumor suppressor gene loci. (bio-protocol.org)
  • Epithelial tumors of nasal cavity, sinuses, nasopharynx: A) Squamous cell carcinoma with variants of nasal cavity, sinuses, and nasopharynx and trachea (excluding laryngeal, nasopharyngeal cancer [NPC], and squamous cell carcinoma of the head and neck [SCCHN]) B) Adenocarcinoma and variants of nasal cavity, sinuses, and nasopharynx (closed to accrual 07/27/2018) 2. (ucbraid.org)
  • Epithelial tumors of penis - squamous adenocarcinoma cell carcinoma with variants of penis (closed to accrual) 18. (ucbraid.org)
  • Strong academic background having completed a first class degree in physiology at Liverpool University where I studied calcium signalling in pancreatic acinar cells. (spirehealthcare.com)
  • Dr Bruce explains: "Insulin works by restoring the energy levels of pancreatic acinar cells, which fuels the calcium pumps on the membrane of these cells. (nutritionreview.org)
  • Neuroblastoma cells expressing mSOD1 had increased cytoplasmic calcium levels and a significant decrease in mitochondrial membrane potential [85]. (bcl2-receptor.com)
  • Calcium supplement Image resolution in Pancreatic Acinar Cells Cells had been packed with Fluo4-Have always been (5 meters) and triggered at room heat with CCK at 5 and 50 pm. (casein-kinases.com)
  • I performed differential gene expression analysis between 12 acinar- and 13 ductal-cell-derived tumors using a specific R programming language package called DESeq2. (ubc.ca)
  • However, there is little study done on the immune microenvironment differences between acinar and ductal cell-derived precursor lesions and PDAC. (ubc.ca)
  • 2. In the mammary glands, the milk-secreting epithelial cells, which are activated during lactation. (unboundmedicine.com)
  • The interaction of the G protein and specific cell surface receptors may be involved. (cdc.gov)
  • Many cell surface receptors , including G protein-coupled receptors and receptor tyrosine kinases , activate the PLC enzyme. (wikipedia.org)
  • As part of the Slit-Robo signaling pathway, Robo receptors work in conjunction with Slit ligands to mediate axon guidance, cell migration, and cell positioning in development. (biorxiv.org)
  • Slit ligand binding to Robo receptors can induce cell migration using repulsive or attractive cues in a context-dependent manner. (biorxiv.org)
  • It also inhibits agonist induced down-regulation of muscarinic receptors in SK-N-SH neuroblastoma cells. (enzolifesciences.com)
  • Nicotinic acidity adenine dinucleotide phosphate (NAADP) is certainly the most powerful Ca2+-mobilizing intracellular messenger and is certainly connected to a variety of stimuli and cell surface area receptors. (casein-kinases.com)
  • We also obtained evidence to show that the pancreatic acinar mitochondrial TPP uptake process is up-regulated in thiamin deficiency via transcriptional mechanism(s), that the process is also under possible regulation by specific intracellular regulatory pathway(s). (nih.gov)
  • Moreover evidence were obtained to show that chronic exposure of pancreatic acinar cells to alcohol and to specific components of CS leads to a significant inhibition in pancreatic acinar mitochondrial TPP uptake process, and that the effect is mediated at the level of SLC25A19 transcription. (nih.gov)
  • Based on these preliminary findings, our working hypothesis in this proposal is that the pancreatic acinar mitochondrial TPP uptake process is a regulated event, that this regulation occurs at both transcriptional and post-transcriptional levels, that unique sequence(s) dictated targeting of MTPPT to mitochondria, and that chronic exposure of pancreatic acinar cells to alcohol and to specific components of CS impairs TPP uptake. (nih.gov)
  • Results of these investigations are expected to provide valuable information regarding physiology/cell biology of an important pancreatic acinar mitochondrial uptake process, information that may also be applicable to other mitochondrial uptake systems. (nih.gov)
  • Furthermore, the results are of pathophysiological relevance as they may shed light onto the consequences of exposure of pancreatic acinar cells to common external factors that adversely affect pancreatic health, on mitochondrial physiology. (nih.gov)
  • Chronic alcohol exposure affects pancreatic acinar mitochondrial thiamin pyrophosphate uptake: studies with mouse 266-6 cell line and primary cells. (nih.gov)
  • Pancreatic neuroendocrine tumors (PanNET)-Come from the islet cells. (epnet.com)
  • Acinar:Kras-p53 mice), which developed tumors originating from ductal or acinar cells, respectively. (ubc.ca)
  • While tumors from both cells of origin were similarly proliferative and shared many physical characteristics, Duct:KPcKO mice developed tumors much earlier than Acinar:KPcKO mice and this was further associated with a difference in precursor lesion initiation. (ubc.ca)
  • What they found was that two different cancer cell types merged in the artificial duct-referred to as the ductal tumor‐microenvironment‐on‐chip (dT‐MOC)-and consequently become more invasive, proceeding to sprout out of the duct and form tumors. (drugdiscoverynews.com)
  • However, in recent studies ( 30 , 31 ), anti-angiogenesis therapy using angiostatin or endostatin to target endothelial cells showed to have little effect on regulating the progression of tumors with VM formation. (spandidos-publications.com)
  • 2012). Moreover, a subset of PDA tumors show epigenetic silencing of endodermal cell fate determinants, including hepatocyte nuclear element 1 homeobox A (HNF1A), HNF1B, HNF4A, and Kruppel-like element 5 (KLF5), in association with a stable epithelial-to-mesenchymal fate transition (David et al. (lxsustentavel.com)
  • 2016). We have recently demonstrated that mouse and human being PDA tumors can upregulate the pioneer element Forkhead package A1 (FOXA1), which leads to the activation of an embryonic foregut endoderm enhancer scenery to endow tumor cells with metastatic potential (Roe et al. (lxsustentavel.com)
  • Recent transcriptome profiling of human being tumor specimens exposed that squamous lineage markers are indicated in as much as 25% of PDA tumors, which includes the adenosquamous tumors as well as specimens that lack clear evidence of this cell morphology (Bailey et al. (lxsustentavel.com)
  • Rare pancreatic tumors including acinar cell carcinoma, mucinous cystadenocarcinoma or serous cystadenocarcinoma. (ucbraid.org)
  • Non-epithelial tumors of the ovary: A) Germ cell tumor of ovary B) Mullerian mixed tumor and adenosarcoma (closed to accrual 03/30/2018) 14. (ucbraid.org)
  • Cell tumor of the testes and extragonadal germ tumors: A) Seminoma and testicular sex cord cancer B) Non seminomatous tumor C) Teratoma with malignant transformation (closed to accrual) 17. (ucbraid.org)
  • Malignant giant cell tumors 30. (ucbraid.org)
  • Studies during the previous funding period have focused exclusively on thiamin transport into pancreatic cells across cell membrane. (nih.gov)
  • The fusion of the rabies virus envelope to the host cell membrane (adsorption) initiates the infection process. (cdc.gov)
  • Conversely, virus in the salivary glands buds primarily from the cell membrane into the acinar lumen. (cdc.gov)
  • Signaling occurs when the cell is stimulated to release Ca 2+ ions from intracellular stores, and/or when Ca 2+ enters the cell through plasma membrane ion channels. (wikipedia.org)
  • In pancreatic acinar cells Ca(2+)-dependent secretagogues promote the fusion of zymogen granules (ZG) with the apical plasma membrane (PM) and exocytosis of digestive enzymes. (koreamed.org)
  • Flipper-TR ® is a live cell fluorescent membrane tension probe that simplifies the methodology for investigating changes in membrane tension when used in combination with standard fluorescence lifetime measurements. (cytoskeleton.com)
  • The loss of aquaporin 5 and possibly other membrane-fusion proteins in acinar cells may be the major mechanism underlying such a dysfunction. (allenpress.com)
  • Vasopressin regulates renal water homeostasis via Aquaporins by regulating the permeability of the epithelium through activation of a signaling cascade leading to the phosphorylation of AQP2 and its translocation from intracellular vesicles to the apical membrane of collecting duct cells. (reactome.org)
  • Ultrastructural changes included membrane-bound vacuoles in hepatocytes, Kupffer cells, acinar pancreatic cells, Purkinje cells, and the small neurons of the granular cell layer of the cerebellum. (conicet.gov.ar)
  • An electron lucent space separates the basal lamina from the plasma membrane of the epithelial cells. (medicalhistology.us)
  • Dexamethasone converts pluripotent pancreatic AR42J cells into exocrine cells expressing digestive enzymes. (koreamed.org)
  • A secretagogue-stimulated pancreatic acinar cell releases digestive enzymes from its apical pole. (uea.ac.uk)
  • 1 Department of Radiation Oncology, 2 Cancer Institute and Institute for Stem Cell Biology and Regenerative Medicine, 3 Department of Otolaryngology (Head and Neck Surgery), and 4 Department of Pathology, Stanford University School of Medicine, Stanford, California, USA. (jci.org)
  • A team at Stanford's Institute for Stem Cell Biology & Regenerative Medicine (ISCBRM) , led by Marcus Wernig and graduate student Thomas Vierbuchen, recently announced that a combination of only three transcription factors that would change a skin cell into a nerve cell - with no intermediate (undifferentiated) steps. (hplusmagazine.com)
  • Developmental Cell is a broad-interest journal covering the areas of cell biology and developmental biology. (cell.com)
  • In addition to publishing work spanning all areas of cell biology and developmental biology, we have particular interest in how these fields interface with each other and with other disciplines in biology. (cell.com)
  • His team has combined genetic model systems and advanced genomics to address key questions in human beta cell biology, regeneration, and disease. (imperial.ac.uk)
  • The deregulation and overexpression as well as genetics variants of this gene have been related to angiogenesis, cell proliferation, metabolism, cancer biology, and osteoarthritis [ 5 , 6 ]. (hindawi.com)
  • Prostate cancer detection following diagnosis of atypical small acinar proliferation. (uchicago.edu)
  • AT1 receptor, which mediates most of the biological results of AT-II action, including vasoconstriction, cell proliferation, and production of extracellular matrix proteins, is found in the kidney, liver, adrenal cortex, and blood vessels. (aspetjournals.org)
  • The Hippo-YAP signaling pathway is mainly involved in cell proliferation and stem cell self-renewal. (medscimonit.com)
  • Fatal horse cases-pancreatic lesions (acinar cells), hematopoietic involvement with or without encephalitis. (cdc.gov)
  • Specifically, ductal cells only formed high-grade lesions while acinar cells formed precursor lesions of all grades. (ubc.ca)
  • It was thought that formation of new blood vessels in tumor lesions depends on vascular endothelial cells. (spandidos-publications.com)
  • Multiple cytoplasm vacuoles in hepatocytes, acinai pancreatic cells, and neurons, especially Purkinje cells, were the most striking microscopic lesions in the five animals. (conicet.gov.ar)
  • I focused on immune cell infiltration densities in precursor lesions and PDAC derived from KPtenΔDuct/+ and KPtenΔAcinar/+ mice and found that immune cell population and its changes throughout tumorigenesis are different starting at a precursor lesion stage between these two models. (ubc.ca)
  • However, the product quality and performance from the purification had not been examined in these prior research, where only main cell types (beta cells, duct cells and acinar cells) had been examined for lectin-affinity21. (aksumuniversity.org)
  • MIST1 expression is normally restricted to pancreatic acinar cells, whereas SOX9 is found exclusively in duct cells. (drugdiscoverynews.com)
  • The relative volume secreted by acinar and duct cells and modification of electrolyte composition of the secreted fluids varies among secretory glands to meet their physiological functions. (bgu.ac.il)
  • Cells located internally in explants, or in central acini in vivo , show this overt differentiation first. (rupress.org)
  • As the epithelia age, the more peripheral cell population proceeds in a similar differentiation. (rupress.org)
  • Some daughter cells apparently return peripherad to divide again, whereas others remain centrally where they undergo differentiation. (rupress.org)
  • The differentiation of a cell, and a cell's commitment to become a particular type, have been long considered irreversible processes. (hplusmagazine.com)
  • Phosphorylation of the retinoblastoma gene product is modulated during the cell cycle and cellular differentiation. (xenbase.org)
  • A hyaluronic acid (HA) based matrix-modified modular hydrogel culture system that fosters growth and differentiation of human parotid gland progenitor cells into functional structures has been established. (christianacare.org)
  • Specifically, we showed that genetic deletion of Robo1 and Robo2 in β-cells ( Robo β KO ) results in loss of stereotypic murine islet architecture, without affecting β-cell differentiation or maturation. (biorxiv.org)
  • Thus, our data demonstrated that the neuroendocrine differentiation that occurs in PCa cells is, at least in part, regulated by FYN kinase. (oncotarget.com)
  • Finally, we find edge-like states in lung, liver, prostate, and colon tissues, suggesting that subpopulations of healthy cells across tissues can exist in pre-cancerous states prior to accumulating mutations. (imsc.res.in)
  • However, in 1999, Maniotis et al ( 4 ) reported that there was a ring-shaped loop interconnecting network from extracellular matrix and melanoma cells to facilitate neoangiogenesis in skin or liver metastasis. (spandidos-publications.com)
  • During Transport of glycerol from adipocytes to the liver by Aquaporins, glycerol generated by triglyceride hydrolysis is exported from adipocytes by AQP7 and is imported into liver cells via AQP9. (reactome.org)
  • Klatskin tumor - Cancer that develops in cells that line the bile ducts in the liver, where the right and left ducts meet. (en-academic.com)
  • The small duct subset is typically peripherally located within the liver parenchyma characterized by glands lined by cuboidal cells embedded in an hypocellular sclerotic stroma. (mayocliniclabs.com)
  • Serine protease inhibitor Kazal-type 1 (SPINK1) is a gene expressed from pancreatic acinar cell which its mutation is known to be associated with chronic pancreatitis (CP) and pancreatic cancer. (koreamed.org)
  • Type I cells are simple, thin squamous epithelial cells. (unboundmedicine.com)
  • VM has been observed in several other aggressive tumor types, such as laryngeal squamous cell carcinoma, ovarian cancer, breast cancer, osteosarcoma, astrocytoma and gallbladder cancer ( 5 - 12 ). (spandidos-publications.com)
  • Simple Squamous Epithelium is composed of flattened polygonal cells. (medicalhistology.us)
  • Simple squamous epithelial cells line the heart and the blood and lymph vessels (where they are called endothelium) as well as the outside of many visceral organs where they are called mesothelium (from embryological mesoderm). (medicalhistology.us)
  • It is composed of very thin sheets of flattened, polyhedral (squamous) cells and thus is called simple squamous epithelium. (medicalhistology.us)
  • 2005). Nonetheless, histological analyses have revealed that a subset of human being PDAs possess an adenosquamous cell morphology, which is definitely invariably associated with the manifestation of TP63, a expert regulator of the normal squamous lineage (Mills et al. (lxsustentavel.com)
  • Squamous cell carcinoma variants of the genitourinary (GU) system 19. (ucbraid.org)
  • Stem cells (embryonic ES or induced iPS cells) have not yet committed to become any particular cell type, and they exist in an entirely undifferentiated stage. (hplusmagazine.com)
  • Fig. 6 6 Effect of substrate stiffness on the morphology and cell arrangement of ex vivo cultured embryonic gland. (archive.org)
  • Histological evaluation uncovered thick stroma and left over duct buildings and a significant decrease in tissues mass (Amount rodents to make certain regular embryonic advancement and growth of acinar cells and give synchronization of Kras account activation while evaluating the results of Numb reduction on the early metaplastic procedure. (cenicriviroc.info)
  • At postnatal time 2, no Rabbit Polyclonal to RFWD2 distinctions are noticed between and pancreata, recommending regular embryonic body organ and cell advancement (Supplemental Amount 7). (cenicriviroc.info)
  • Our goal is to restore salivary gland function using human salivary progenitor cells reconstituted in modular hydrogel form into patients with xerostomia. (christianacare.org)
  • Here, we developed a bottom-up approach for generating salivary gland microtissues using primary human salivary myoepithelial cells (hSMECs) and stem/progenitor cells (hS/PCs) isolated from normal salivary gland tissues. (archive.org)
  • After discovering that the green tea polyphenol epigallocatechin-3-gallate (EGCG) inhibits autoantigen expression in cultured human keratinocytes and immortalized normal human salivary acinar cells, Dr Hsu and his associates studied the effect of the compound in an animal model of the disease. (lifeextension.com)
  • Histomorphologically, the tumour resembles the cells of the pancreatic acini and, typically, have moderate granular cytoplasm that stain with both PAS and PASD. (wikipedia.org)
  • After adsorption, the virus penetrates the host cell and enters the cytoplasm. (cdc.gov)
  • There are small, clear spaces (vacuoles) in the cytoplasm of the acinar cells (arrows). (nih.gov)
  • Histologically, cells are enlarged and the cytoplasm of affected cells contains translucent vacuoles. (nih.gov)
  • Paget cells have voluminous clear, pale eosinophilic or foamy cytoplasm . (webpathology.com)
  • Figure 3: The neoplastic cells show ample eosinophilic cytoplasm and large, round, pleomorphic nuclei with prominent nucleoli. (mayocliniclabs.com)
  • Cytologically, neoplastic cells are usually large, characterized by abundant eosinophilic cytoplasm and large nuclei with vesicular chromatin, and occasional prominent nucleoli. (mayocliniclabs.com)
  • Basal autophagy maintains pancreatic acinar cell homeostasis and protein synthesis and prevents ER stress. (ucsd.edu)
  • Keratin filaments (tonofilaments, arrows) are sparse, and desmosomes are few (see inset), in the basal cell layer of this tissue. (cellimagelibrary.org)
  • Some cells which do not appear to be lining a surface (namely the secretory parenchyma of glands) are also epithelial by definition (little intercellular space, avascular, junctional complexes, basal lamina). (medicalhistology.us)
  • Basal cell carcinoma (temporarily closed to accrual 04/29/2020) 42. (ucbraid.org)
  • RATIONALE: Vaccines made from gene-modified pancreatic cancer cells may make the body build an immune response to kill tumor cells. (clinicaltrials.gov)
  • Drugs used in chemotherapy, such as fluorouracil, work in different ways to stop tumor cells from dividing so they stop growing or die. (clinicaltrials.gov)
  • Radiation therapy uses high-energy x-rays to damage tumor cells. (clinicaltrials.gov)
  • Giving vaccine therapy together with chemotherapy and radiation therapy after surgery may kill any remaining tumor cells. (clinicaltrials.gov)
  • The tumor cells may extend into the adnexal structures. (webpathology.com)
  • To determine whether the expression pattern was specific to III-tubulin, we also AUY922 inhibitor examined the levels of another -tubulin isotype, II-tubulin, which has been shown to be differentially expressed in tumor cells [27-29]. (cylch.org)
  • It too was present at high levels in pancreatic tumor cells, however in contrast to III-tubulin, it was also present in acinar and normal ductal cells (Supplementary Figure 1). (cylch.org)
  • Immunotherapy with monoclonal antibodies, such as nivolumab and ipilimumab, may help the body's immune system attack the cancer, and may interfere with the ability of tumor cells to grow and spread. (ucbraid.org)
  • This increase was localized primarily to the acinar cells and the ductal cells by immunohistochemistry. (elsevier.com)
  • Open in a separate window Figure 1 III-tubulin expression in PDA patient tissue and PDA cell linesA) Immunohistochemistry for III-tubulin in a representative human AUY922 inhibitor PDA tissue specimen. (cylch.org)
  • In contrast, Acinar:Kras-p53 mice formed abundant of low grade PanINs with mucinous characteristics and PDAC initiation was delayed. (ubc.ca)
  • Following our previous study that showed loss of Pten with oncogenic KrasG12D mutations in the ductal cells (KPtenΔDuct/+) resulted the formation of intraductal papillary mucinous neoplasias (IPMN) as the precursor lesion in mice, we further found similar mutations in the acinar cells (KPtenΔAcinar/+) formed pancreatic intraepithelial neoplasia (PanIN) instead. (ubc.ca)
  • Observations of autoradiograms of H 3 -thymidine-labeled tissues indicate that the first cells which cease incorporating the DNA-precursor are in the central regions that differentiate first. (rupress.org)
  • A precursor cell that can also give rise to identical precursor cells. (unboundmedicine.com)
  • We hypothesized that pancreatic ducts contain precursor or parent cells that can be activated to form new beta cells. (uwo.ca)
  • The renal tubules showed lined cells, in some instances basophilic with presence of exfoliated cells and/or amorphous material (crystal-like) in their lumen. (europa.eu)
  • After X irradiation (single dose, 15 Gy), a vacuolation in the acinar cells or an enlargement of the acinar lumen was observed as a typical morphological change for 2 weeks. (allenpress.com)
  • Certainly, 2 times after caerulein treatment, rodents provided with improved acinar dedifferentiation and elevated lumen size (Amount 2pancreata demonstrated even more obvious stromal extension and resistant cell infiltration, as sized by elevated existence of -Even Muscles Compact disc45 and Actin positive cells, respectively (Supplementary Amount 4). (cenicriviroc.info)
  • Likened to mutants, acini shown an improved response to caerulein as showed by elevated lumen size, expanded reduction of the acinar cell gun Cpa1 and significant induction in CK19 reflection at time 2 after caerulein (Amount 4mglaciers (Amount 4pancreata pursuing caerulein treatment. (cenicriviroc.info)
  • However, these mice could not be used in studies in which acinar cells need to be isolated from mice with additional Cre-mediated genetic modifications, e.g. gene ablation or overexpression. (aksumuniversity.org)
  • A tissue-specific Knockout mouse defines an animal model in which a gene of interest is ' floxed ' and thus inactivatable in specific cell types in a certain tissue. (genoway.com)
  • Other cell types and tissues exhibit an unmodified, functional gene expression. (genoway.com)
  • This gene inactivation is achieved by an additional breeding step with a tissue- or cell type-specific Cre-deleter mouse line. (genoway.com)
  • However, when oncogenic KRAS is expressed in acinar cells, the MIST1 gene is rapidly silenced while the SOX9 gene is activated in this cell type," as reported on the Konieczny lab website. (drugdiscoverynews.com)
  • The retinoblastoma-susceptibility gene product becomes phosphorylated in multiple stages during cell cycle entry and progression. (xenbase.org)
  • Enhanced pathogenicity of diabetogenic T cells escaping a non-MHC gene-controlled near death experience. (umassmed.edu)
  • In male mice fed Roundup Ready soybeans, the structure and gene expression pattern of testicular cells changed significantly. (rawfoodlife.com)
  • We invite you to discover Human Gene Therapy Clinical Development, a peer-reviewed journal publishing preclinical research and clinical trial data for gene and cell therapies. (upenn.edu)
  • The cell/gene matrix and cell-level metadata was downloaded from the UCSC Cell Browser . (ucsc.edu)
  • The data allow for direct and controlled comparison of gene expression in cell types shared between tissues, such as immune cells from distinct anatomical locations. (ucsc.edu)
  • A rectangle on the genome, at the location of a gene, filled with a bar graph that indicates the gene's expression by single cell cluster. (ucsc.edu)
  • Look for cells arranged in a single layer surrounding a glomerulus in the kidney. (medicalhistology.us)
  • Spindle cell carcinoma of kidney, pelvis, ureter 20. (ucbraid.org)
  • We show that function of either Slit2 or Slit3, which are predominantly expressed in the pancreatic mesenchyme, is required and sufficient for islet morphogenesis, while Slit1, which is predominantly expressed in the β cells, is dispensable for islet morphogenesis. (biorxiv.org)
  • In older explants, thymidine incorporation is largely restricted to the periphery of the tissue as zymogen appears in the internal cells. (rupress.org)
  • Such adaptation enables the reactivation of acinar-to-ductal metaplasia (ADM) upon subsequent inflammatory events, thereby limiting tissue damage through a rapid decrease of zymogen production. (nih.gov)
  • Candesartan significantly suppressed decrease in pancreatic weight and increases in pancreatic myeloperoxidase activity, hydroxyproline content, ratio of fibrous tissue, histologic scores, and ratio of α-smooth muscle actin-positive cells (activated pancreatic stellate cells) at 20 weeks. (aspetjournals.org)
  • We further studied in depth the non-cancerous tissue and developed a three-dimensional (3D) imaging protocol (FLIP-IT, Fluorescence Light sheet microscopic Imaging of Paraffin-embedded or Intact Tissue) to study formalin-fixed paraffin-embedded samples at single cell resolution. (bmj.com)
  • Encapsulation of Primary Salivary Gland Acinar Cell Clusters and Intercalated Ducts (AIDUCs) within Matrix Metalloproteinase (MMP)-Degradable Hydrogels to Maintain Tissue Structure and Function. (rochester.edu)
  • The journal aims to showcase work that examines, through a cell biological lens, the fundamentals of how a cell works, how cells interact with one another and with the environment, how cells come together to form a tissue, organ, and ultimately a whole organism, and what happens when any of these processes go awry. (cell.com)
  • We also found that the fraction of edge-like cells increased with age in healthy pancreatic tissue, even though they did not possess any clonal oncogenic mutations. (imsc.res.in)
  • Mebendazole significantly reduced trichrome-positive fibrotic connective tissue and α-SMA-positive activated pancreatic stellate cells that heralds fibrogenesis. (oncotarget.com)
  • In Sjögren's, autoreactive lymphocytes (a type of white blood cell) infiltrate and may destroy the glandular tissue and affect gland function. (healthywomen.org)
  • developed methods for isolating salivary gland acinar-like cell populations from tissue specimens harvested from patients undergoing head and neck surgery. (archive.org)
  • Phenotypic characterization of isolated hSMECs confirmed that a myoepithelial cell phenotype consistent with that from other exocrine tissues was maintained over multiple passages of tissue culture. (archive.org)
  • In ovarian carcinoma tissue homogeneous staining of epithelial cells was observed with OV-TL 3 and more heterogeneous staining with MOv18. (bmj.com)
  • The term 'cluster' refers to a cluster of single cells, which usually represents a cell or tissue type. (ucsc.edu)
  • It is predominantly a cellular tissue with little intervening space between adjacent cells. (medicalhistology.us)
  • It consists of two parts: 1) a layer of collagen (type IV) and amorphous ground substance secreted by the epithelial cells, and 2) reticular fibers which extend into the ground substance from the underlying connective tissue. (medicalhistology.us)
  • hematopoietic stem cells can become any type of cell in the blood. (hplusmagazine.com)
  • The investigational treatment will involve 2 cycles of a combination of intravenous melphalan, BCNU, low-dose I.V. ethanol, vitamin B12b, and vitamin C in association with autologous hematopoietic stem cell infusion. (centerwatch.com)
  • Mobilization of hematopoietic stem cells will only occur prior to the first cycle of investigational therapy. (centerwatch.com)
  • It is thus justified to conclude that replenishment of insulin-producing pancreatic β-cells is crucial for treating diabetes and its complications [ 5 ], thereby overcoming the inadequacies of current treatment strategies. (biomedcentral.com)
  • What makes the Manchester research unique is that it provides the first evidence that insulin directly protects the acinar cells, which is where the disease is initiated. (nutritionreview.org)
  • Just like with insulin and leptin, our cells can become resistant to adrenaline. (robbwolf.com)
  • The conclusion is that candesartan alleviates chronic pancreatitis and fibrosis by suppressing the overexpression of transforming growth factor-β1, resulting in prevention of activation of pancreatic stellate cells in male WBN/Kob rats. (aspetjournals.org)
  • Myoepithelial cells are flat, stellate cells present in exocrine tissues including the salivary glands. (archive.org)
  • Both acinar and ductal cells can give rise to PDAC in murine models. (ubc.ca)
  • Laser Capture Microdissection of Pancreatic Acinar Cells to Identify Proteomic Alterations in a Murine Model of Caerulein-Induced Pancreatitis. (wjgnet.com)
  • RWPE-2 cells were derived from RWPE-1 cells ( ATCC CRL-11609 ) by transformation with Ki-ras using the Kirsten murine sarcoma virus (Ki-MuSV). (atcc.org)
  • Isolation of Acalisib (GS-9820) acinar cells using genetically altered mice in which Acalisib (GS-9820) a fluorescent reporter is definitely indicated under an acinar-cell-specific promoter offers its advantages and disadvantages. (aksumuniversity.org)
  • We created PDAC cell lines from mouse models (Sox9CreER;KrasLSL-G12D;Trp53f/f mice a.k.a. (ubc.ca)
  • Duct:Kras-p53 mice formed high grade PanINs, developed PDAC much faster, and had a shorter lifespan compared to Acinar:Kras-p53 mice. (ubc.ca)
  • I found that Duct:KPcKO mice met their humane endpoints earlier (82 days post injection, p.i.) than the Acinar:KPcKO mice (128 days p.i.), for reasons associated with differences in the timing of PDAC onset. (ubc.ca)
  • Mice that lack Atg16l1 in intestinal epithelial cells (IECs) ( Atg16l1 ΔIEC ) phenocopying ileal CD observed in humans homozygous for ATG16L1 T300A . (genoway.com)
  • IRE1α accumulates in Paneth cells, specialized IECs, of Atg16l1 ΔIEC mice, and humans homozygous for ATG16L1 T300A exhibit a corresponding increase of IRE1α in intestinal epithelial crypts. (genoway.com)
  • During the early prediabetic period in NOD mice, the pathogenic CD8(+) T-cell population comprises multiple antigenic specificities. (umassmed.edu)
  • HLA-A*0201-restricted T cells from humanized NOD mice recognize autoantigens of potential clinical relevance to type 1 diabetes. (umassmed.edu)
  • Subcongenic analysis of genetic basis for impaired development of invariant NKT cells in NOD mice. (umassmed.edu)
  • Idd9/11 genetic locus regulates diabetogenic activity of CD4 T-cells in nonobese diabetic (NOD) mice. (umassmed.edu)
  • cell neo-self-antigen in type 1 diabetes prone NOD mice. (umassmed.edu)
  • Prevention of "Humanized" diabetogenic CD8 T-cell responses in HLA-transgenic NOD mice by a multipeptide coupled-cell approach. (umassmed.edu)
  • Functional evidence for the mediation of diabetogenic T cell responses by HLA-A2.1 MHC class I molecules through transgenic expression in NOD mice. (umassmed.edu)
  • Tamoxifen treatment in mice induced labelling of CK19+ ductal and acinar cells with tdTomato and streptozotocin (50 mg/kg/day x 5 days) induced hyperglycemia. (uwo.ca)
  • Experimental pancreatitis in keratin-null mice reveals major differences in pancreatic acinar cell and hepatocyte keratin cytoprotective functions. (abo.fi)
  • Abstract] Measuring antigen-specific T cell responses in the blood and lymphoid organs of vaccinated mice can give us a useful indication of the potency of a vaccine formulation. (bio-protocol.org)
  • MATERIALS AND METHODS Pancreatic Acinar Cell Preparation Male C57BL6 mice (CD38 sufficient) were purchased from CER Janvier and male C57BL/6 CD38? (casein-kinases.com)
  • Only stem cells can become any type of cell in a particular organism -- the property of pluripotency. (hplusmagazine.com)
  • There are occasional switches between types of less differentiated adult stem cells, cancer cells inexplicably de-differentiate, and scientists have been able to revert cells to an entirely undifferentiated, induced pluripotent stem (iPS) cell state in a lab - but the closer a cell gets to a terminally differentiated stage, the less able it is to change course. (hplusmagazine.com)
  • And the unlimited potential of pluripotency is exclusive to cells at the start of all lineages - undifferentiated stem cells alone may become any type of cell. (hplusmagazine.com)
  • Only stem cells have been known to do this. (hplusmagazine.com)
  • Granulocyte colony-stimulating factor, and if needed Plerixafor, will be used to mobilize bone marrow stem cells, which will be collected by apheresis. (centerwatch.com)
  • If there is not a sufficient mobilization of stem cells for at least 2 cycles of chemotherapy, then no investigational drugs will be given. (centerwatch.com)
  • Nanoparticle-Mediated Delivery of Micheliolide Analogs to Eliminate Leukemic Stem Cells in the Bone Marrow. (rochester.edu)
  • Derivation of induced pluripotent stem cells from orangutan skin fibroblasts BMC Res Notes. (usc.edu)
  • The presence of a post-DNA-synthetic population is seen in the form of a group of nonlabeling central cells that remains intact in the midst of a labeled epithelium for as long as 48 hours in vitro (from 72 to 120 hours). (rupress.org)
  • In 2008, a Japanese team transdifferentiated pancreatic acinar to beta cells in vitro. (hplusmagazine.com)
  • Upon recovery from acute inflammation, pancreatic epithelial cells display an enduring adaptive response associated with sustained transcriptional and epigenetic reprogramming. (nih.gov)
  • The fraction of edge-like cells is also significantly higher in human pancreatitis samples, suggesting a common cell state underlying both inflammation and recovery from injury, and pancreatic cancer. (imsc.res.in)
  • TRPV1+ sensory neurons control beta cell stress and islet inflammation in autoimmune diabetes. (umassmed.edu)
  • The team suspects that EGCG activates the body's defense system again tumor necrosis factor-alpha, a protein produced by the white blood cells during inflammation. (lifeextension.com)
  • Using an immunohistochemical method, no significant change was observed in amylase distribution, but a marked loss of aquaporin 5 was found in the acinar cells after the irradiation. (allenpress.com)
  • 2017). Collectively, these studies spotlight aberrant cell fate transitions like a hallmark house of PDA, which can be recognized mechanistically by epigenomic mapping of the global enhancer construction. (lxsustentavel.com)