Acidithiobacillus thiooxidans: A strictly autotrophic species of bacteria that oxidizes sulfur and thiosulfate to sulfuric acid. It was formerly called Thiobacillus thiooxidans.Acidithiobacillus: A genus of gram-negative rod-shaped bacteria in the class GAMMAPROTEOBACTERIA. They are obligately acidophilic and aerobic, using reduced SULFUR COMPOUNDS to support AUTOTROPHIC GROWTH.Tungsten Compounds: Inorganic compounds that contain tungsten as an integral part of the molecule.Thiobacillus: A genus of gram-negative, rod-shaped bacteria that derives energy from the oxidation of one or more reduced sulfur compounds. Many former species have been reclassified to other classes of PROTEOBACTERIA.Sulfur: An element that is a member of the chalcogen family. It has an atomic symbol S, atomic number 16, and atomic weight [32.059; 32.076]. It is found in the amino acids cysteine and methionine.Corrosion: The gradual destruction of a metal or alloy due to oxidation or action of a chemical agent. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Hydrogen Sulfide: A flammable, poisonous gas with a characteristic odor of rotten eggs. It is used in the manufacture of chemicals, in metallurgy, and as an analytical reagent. (From Merck Index, 11th ed)Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)MiningPulpotomy: Dental procedure in which part of the pulp chamber is removed from the crown of a tooth.Sulfides: Chemical groups containing the covalent sulfur bonds -S-. The sulfur atom can be bound to inorganic or organic moieties.Sulfuric Acids: Inorganic and organic derivatives of sulfuric acid (H2SO4). The salts and esters of sulfuric acid are known as SULFATES and SULFURIC ACID ESTERS respectively.Thorium Dioxide: Thorium oxide (ThO2). A radiographic contrast agent that was used in the early 1930s through about 1954. High rates of mortality have been linked to its use and it has been shown to cause liver cancer.Foundations: Organizations established by endowments with provision for future maintenance.Yersinia pestis: The etiologic agent of PLAGUE in man, rats, ground squirrels, and other rodents.Mononuclear Phagocyte System: Mononuclear cells with pronounced phagocytic ability that are distributed extensively in lymphoid and other organs. It includes MACROPHAGES and their precursors; PHAGOCYTES; KUPFFER CELLS; HISTIOCYTES; DENDRITIC CELLS; LANGERHANS CELLS; and MICROGLIA. The term mononuclear phagocyte system has replaced the former reticuloendothelial system, which also included less active phagocytic cells such as fibroblasts and endothelial cells. (From Illustrated Dictionary of Immunology, 2d ed.)Oncogenic Viruses: Viruses that produce tumors.Brassica: A plant genus of the family Cruciferae. It contains many species and cultivars used as food including cabbage, cauliflower, broccoli, Brussel sprouts, kale, collard greens, MUSTARD PLANT; (B. alba, B. junica, and B. nigra), turnips (BRASSICA NAPUS) and rapeseed (BRASSICA RAPA).Stevens-Johnson Syndrome: Rare cutaneous eruption characterized by extensive KERATINOCYTE apoptosis resulting in skin detachment with mucosal involvement. It is often provoked by the use of drugs (e.g., antibiotics and anticonvulsants) or associated with PNEUMONIA, MYCOPLASMA. It is considered a continuum of Toxic Epidermal Necrolysis.Peer Group: Group composed of associates of same species, approximately the same age, and usually of similar rank or social status.Video Games: A form of interactive entertainment in which the player controls electronically generated images that appear on a video display screen. This includes video games played in the home on special machines or home computers, and those played in arcades.Videotape Recording: Recording of visual and sometimes sound signals on magnetic tape.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Journal Impact Factor: A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.Peer Review, Research: The evaluation by experts of the quality and pertinence of research or research proposals of other experts in the same field. Peer review is used by editors in deciding which submissions warrant publication, by granting agencies to determine which proposals should be funded, and by academic institutions in tenure decisions.Peer Review: An organized procedure carried out by a select committee of professionals in evaluating the performance of other professionals in meeting the standards of their specialty. Review by peers is used by editors in the evaluation of articles and other papers submitted for publication. Peer review is used also in the evaluation of grant applications. It is applied also in evaluating the quality of health care provided to patients.Bacteriology: The study of the structure, growth, function, genetics, and reproduction of bacteria, and BACTERIAL INFECTIONS.Vibrio cholerae: The etiologic agent of CHOLERA.Vibrio: A genus of VIBRIONACEAE, made up of short, slightly curved, motile, gram-negative rods. Various species produce cholera and other gastrointestinal disorders as well as abortion in sheep and cattle.Publishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.Vibrio cholerae O1: Strains of VIBRIO CHOLERAE containing O ANTIGENS group 1. All are CHOLERA-causing strains (serotypes). There are two biovars (biotypes): cholerae and eltor (El Tor).Rhodium: Rhodium. A hard and rare metal of the platinum group, atomic number 45, atomic weight 102.905, symbol Rh. (Dorland, 28th ed)Platinum: Platinum. A heavy, soft, whitish metal, resembling tin, atomic number 78, atomic weight 195.09, symbol Pt. (From Dorland, 28th ed) It is used in manufacturing equipment for laboratory and industrial use. It occurs as a black powder (platinum black) and as a spongy substance (spongy platinum) and may have been known in Pliny's time as "alutiae".Metals: Electropositive chemical elements characterized by ductility, malleability, luster, and conductance of heat and electricity. They can replace the hydrogen of an acid and form bases with hydroxyl radicals. (Grant & Hackh's Chemical Dictionary, 5th ed)Architectural Accessibility: Designs for approaching areas inside or outside facilities.Platinum Compounds: Inorganic compounds which contain platinum as the central atom.Phosphoglycerate Mutase: An enzyme that catalyzes the conversion of 2-phospho-D-glycerate to 3-phospho-D-glycerate. EC 5.4.2.1.Organizational Case Studies: Descriptions and evaluations of specific health care organizations.Data Mining: Use of sophisticated analysis tools to sort through, organize, examine, and combine large sets of information.Solutions: The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed)Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Coal MiningWater: A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Apatites: A group of phosphate minerals that includes ten mineral species and has the general formula X5(YO4)3Z, where X is usually calcium or lead, Y is phosphorus or arsenic, and Z is chlorine, fluorine, or OH-. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Quartz: Quartz (SiO2). A glassy or crystalline form of silicon dioxide. Many colored varieties are semiprecious stones. (From Grant & Hackh's Chemical Dictionary, 5th ed)Aluminum Silicates: Any of the numerous types of clay which contain varying proportions of Al2O3 and SiO2. They are made synthetically by heating aluminum fluoride at 1000-2000 degrees C with silica and water vapor. (From Hawley's Condensed Chemical Dictionary, 11th ed)Surface Properties: Characteristics or attributes of the outer boundaries of objects, including molecules.Microscopy, Atomic Force: A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. These deflections produce a topographic map of the sample.Adhesiveness: A property of the surface of an object that makes it stick to another surface.Amines: A group of compounds derived from ammonia by substituting organic radicals for the hydrogens. (From Grant & Hackh's Chemical Dictionary, 5th ed)

Interaction-induced redox switch in the electron transfer complex rusticyanin-cytochrome c(4). (1/36)

The blue copper protein rusticyanin isolated from the acidophilic proteobacterium Thiobacillus ferrooxidans displays a pH-dependent redox midpoint potential with a pK value of 7 on the oxidized form of the protein. The nature of the alterations of optical and EPR spectra observed above the pK value indicated that the redox-linked deprotonation occurs on the epsilon-nitrogen of the histidine ligands to the copper ion. Complex formation between rusticyanin and its probable electron transfer partner, cytochrome c(4), induced a decrease of rusticyanin's redox midpoint potential by more than 100 mV together with spectral changes similar to those observed above the pK value of the free form. Complex formation thus substantially modifies the pK value of the surface-exposed histidine ligand to the copper ion and thereby tunes the redox midpoint potential of the copper site. Comparisons with reports on other blue copper proteins suggest that the surface-exposed histidine ligand is employed as a redox tuning device by many members of this group of soluble electron carriers.  (+info)

Effect of various ions, pH, and osmotic pressure on oxidation of elemental sulfur by Thiobacillus thiooxidans. (2/36)

The oxidation of elemental sulfur by Thiobacillus thiooxidans was studied at pH 2.3, 4.5, and 7.0 in the presence of different concentrations of various anions (sulfate, phosphate, chloride, nitrate, and fluoride) and cations (potassium, sodium, lithium, rubidium, and cesium). The results agree with the expected response of this acidophilic bacterium to charge neutralization of colloids by ions, pH-dependent membrane permeability of ions, and osmotic pressure.  (+info)

The chromosomal arsenic resistance genes of Thiobacillus ferrooxidans have an unusual arrangement and confer increased arsenic and antimony resistance to Escherichia coli. (3/36)

The chromosomal arsenic resistance genes of the acidophilic, chemolithoautotrophic, biomining bacterium Thiobacillus ferrooxidans were cloned and sequenced. Homologues of four arsenic resistance genes, arsB, arsC, arsH, and a putative arsR gene, were identified. The T. ferrooxidans arsB (arsenite export) and arsC (arsenate reductase) gene products were functional when they were cloned in an Escherichia coli ars deletion mutant and conferred increased resistance to arsenite, arsenate, and antimony. Therefore, despite the fact that the ars genes originated from an obligately acidophilic bacterium, they were functional in E. coli. Although T. ferrooxidans is gram negative, its ArsC was more closely related to the ArsC molecules of gram-positive bacteria. Furthermore, a functional trxA (thioredoxin) gene was required for ArsC-mediated arsenate resistance in E. coli; this finding confirmed the gram-positive ArsC-like status of this resistance and indicated that the division of ArsC molecules based on Gram staining results is artificial. Although arsH was expressed in an E. coli-derived in vitro transcription-translation system, ArsH was not required for and did not enhance arsenic resistance in E. coli. The T. ferrooxidans ars genes were arranged in an unusual manner, and the putative arsR and arsC genes and the arsBH genes were translated in opposite directions. This divergent orientation was conserved in the four T. ferrooxidans strains investigated.  (+info)

Development and application of small-subunit rRNA probes for assessment of selected Thiobacillus species and members of the genus Acidiphilium. (4/36)

Culture-dependent studies have implicated sulfur-oxidizing bacteria as the causative agents of acid mine drainage and concrete corrosion in sewers. Thiobacillus species are considered the major representatives of the acid-producing bacteria in these environments. Small-subunit rRNA genes from all of the Thiobacillus and Acidiphilium species catalogued by the Ribosomal Database Project were identified and used to design oligonucleotide DNA probes. Two oligonucleotide probes were synthesized to complement variable regions of 16S rRNA in the following acidophilic bacteria: Thiobacillus ferrooxidans and T. thiooxidans (probe Thio820) and members of the genus Acidiphilium (probe Acdp821). Using (32)P radiolabels, probe specificity was characterized by hybridization dissociation temperature (T(d)) with membrane-immobilized RNA extracted from a suite of 21 strains representing three groups of bacteria. Fluorochrome-conjugated probes were evaluated for use with fluorescent in situ hybridization (FISH) at the experimentally determined T(d)s. FISH was used to identify and enumerate bacteria in laboratory reactors and environmental samples. Probing of laboratory reactors inoculated with a mixed culture of acidophilic bacteria validated the ability of the oligonucleotide probes to track specific cell numbers with time. Additionally, probing of sediments from an active acid mine drainage site in Colorado demonstrated the ability to identify numbers of active bacteria in natural environments that contain high concentrations of metals, associated precipitates, and other mineral debris.  (+info)

Purification and properties of thiosulfate dehydrogenase from Acidithiobacillus thiooxidans JCM7814. (5/36)

A key enzyme of the thiosulfate oxidation pathway in Acidithiobacillus thiooxidans JCM7814 was investigated. As a result of assaying the enzymatic activities of thiosulfate dehydrogenase, rhodanese, and thiosulfate reductase at 5.5 of intracellular pH, the activity of thiosulfate dehydrogenase was measured as the key enzyme. The thiosulfate dehydrogenase of A. thiooxidans JCM7814 was purified using three chromatographies. The purified sample was electrophoretically homogeneous. The molecular mass of the enzyme was 27.9 kDa and it was a monomer. This enzyme had cytochrome c. The optimum pH and temperature of this enzyme were 3.5 and 35 degrees C. The enzyme was stable in the pH range from 5 to 7, and it was stable up to 45 degrees C. The isoelectric point of the enzyme was 8.9. This enzyme reacted with thiosulfate as a substrate. The Km was 0.81 mM.  (+info)

Purificantion and characterization of inorganic pyrophosphatase from Thiobacillus thiooxidans. (6/36)

An inorganic pyrophosphatase [EC 3.6.1.1] was isolated from Thiobacillus thiooxidans and purified 975-fold to a state of apparent homogeneity. The enzyme catalyzed the hydrolysis of inorganic pyrophosphate and no activity was found with a variety of other phosphate esters. The cation Mg2+ was required for maximum activity; Co2+ and Mn2+ supported 25 per cent and 10.6 per cent of the activity with Mg2+, respectively. The pH optimum was 8.8. The molecular weight was estimated to be 88,000 by gel filtration and SDS gel electrophoresis, and the enzyme consisted of four identical subunits. The isoelectric point was found to be 5.05. The enzyme was exceptionally heat-stable in the presence of 0.01 M Mg2+.  (+info)

Analysis of differential-expressed proteins of Acidithiobacillus ferrooxidans grown under phosphate starvation. (7/36)

Acidithiobacillus ferrooxidans is one of the most important bacterium used in bioleaching, and can utilize Fe(2+) or sulphide as energy source. Growth curves for Acidithiobacillus ferrooxidans under phosphate starvation and normal condition have been tested, showing lag, logarithmic, stationary and aging phases as seen in other bacteria. The logarithmic phases were from 10 to 32 hours for Acidithiobacillus ferrooxidans cultivated with normal cultivating condition and from 20 to 60 hrs for Acidithiobacillus ferrooxidans cultivated phosphate starvation. Differences of protein patterns of Acidithiobacillus ferrooxidans growing in case of normal or phosphate starvation were separately investigated after cultivation at 30(o)C by the analysis of two-dimensional gel electrophoresis (2-DE), matrix-assisted laser desorption/ionization (MALDI)-Mass spectrometry. There were total 6 protein spots identified, which were Recombination protein recA, RNA helicase, AP2 domain-containing transcription factor, NADH dehydrogenase I chain D, Hyothetical protein PF1669, and Transaldolase STY3758. From the 6 identified protein spots, 3 proteins were found to be decreased in expression at the cultivating condition of phosphate starvation, while another three upregulated.  (+info)

Simultaneous removal of H2S and NH3 in biofilter inoculated with Acidithiobacillus thiooxidans TAS. (8/36)

H2S and NH3 gases are toxic, corrosive and malodorous air pollutants. Although there are numerous well-established physicochemical techniques presently available for the treatment of these gases, the growing demand for a more economical and improved process has prompted investigations into biological alternatives. In biological treatment methods, H2S is oxidized to SO4(2-) by sulfur-oxidizing bacteria, and then NH3 is removed by chemical neutralization with SO4(2-) to (NH4)2SO4. Since the accumulated (NH4)2SO4 can inhibit microbial activity, it is important to utilize an effective sulfur-oxidizing bacterium that has tolerance to high concentrations of (NH4)2SO4 for the simultaneous removal of H2S and NH3. In this study, a sulfur-oxidizing bacterium with tolerance to high concentrations of (NH4)2SO4 was isolated from activated sludge and identified as Acidithiobacillus thiooxidans TAS. A. thiooxidans TAS could display its sulfur-oxidizing activity in a medium supplemented with 60 g.l(-1) (NH4)2SO4, even though its growth and sulfur-oxidizing activity were completely inhibited in 80 g.l(-1) (NH4)2SO4. When H2S alone was supplied to a ceramic biofilter inoculated with A. thiooxidans TAS, an almost 100% H2S removal efficiency was maintained until the inlet H2S concentration was increased up to 900 microl.l(-1) and the space velocity up to 500 h(-1), at which the amount of H2S eliminated was 810 g-S.m(-3).h(-1). However, when NH3 (50-500 microl.l(-1)) was simultaneously supplied to the biofilter with H2S, the maximum amount of H2S eliminated decreased to 650 g-S.m(-3).h(-1). The inhibition of H2S removal by low NH3 concentrations (50-200 microl.l(-1)) was similar to that by high NH3 concentrations (300-500 microl.l(-1)). The critical inlet H2S load that resulted in over 99% removal was determined as 400 g-S.m(-3).h(-1) in the presence of NH3.  (+info)

Zn-Mobil is produced from a beneficial autotrophic and acidophilic bacterium Thiobacillus thiooxidans. Zinc (Zn) is one of the essential micronutrient...
Oxidative dissolution of chalcopyrite at ambient temperatures is generally slow and subject to passivation, posing a major challenge for developing bioleaching applications for this recalcitrant mineral. Chloride is known to enhance the chemical leaching of chalcopyrite, but much of this effect has been demonstrated at elevated temperatures. This study was undertaken to test whether 100-200 mM Na-chloride enhances the chemical and bacterial leaching of chalcopyrite in shake flasks and stirred tank bioreactor conditions at mesophilic temperatures. Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and abiotic controls were employed for the leaching experiments. Addition of Na-chloride to the bioleaching suspension inhibited the formation of secondary phases from chalcopyrite and decreased the Fe(III) precipitation. Neither elemental S nor secondary Cu-sulfides were detected in solid residues by X-ray diffraction. Chalcopyrite leaching was enhanced when the solution contained bacteria, ...
Some sulfate-reducing bacteria produce hydrogen sulfide, which can cause sulfide stress cracking. Acidithiobacillus bacteria produce sulfuric acid; Acidothiobacillus thiooxidans frequently damages sewer pipes. Ferrobacillus ferrooxidans directly oxidizes iron to iron oxides and iron hydroxides; the rusticles forming on the RMS Titanic wreck are caused by bacterial activity. Other bacteria produce various acids, both organic and mineral, or ammonia. In presence of oxygen, aerobic bacteria like Acidithiobacillus thiooxidans, Thiobacillus thioparus, and Thiobacillus concretivorus, all three widely present in the environment, are the common corrosion-causing factors resulting in biogenic sulfide corrosion. Without presence of oxygen, anaerobic bacteria, especially Desulfovibrio and Desulfotomaculum, are common. Desulfovibrio salixigens requires at least 2.5% concentration of sodium chloride, but D. vulgaris and D. desulfuricans can grow in both fresh and salt water. D. africanus is another common ...
references say that the former increases the rate of pyrite oxidation causing lakes to be come acidic. While other resources say its the latter. Maybe they are the same? but if they are the same, how come the former also increases the rate of metal precipitation and reduces the acidity of acidic lakes by reduction of sulfate when it causes the acidity in the first place ...
Uporaba mikroorganizmov pri pridobivanju kovin iz mineralnih rud oz. biorudarstvo se je v preteklih nekaj letih razvilo v uspešno in rastočo biotehnološko panogo, kljub temu pa je bila selekcija in spremljanje mikrobnih kultur za optimalno izluževanje minimalna[3]. Vprašanje torej ostaja, ali so trenutne mikrobne populacije v komercialnih postopkih dovolj primerne ali pa bi lahko z umetnim sestavljanjem bakterijskih sevov ustvarili še bolj učinkovite konzorcije[3]. Raziskave so pokazale, da imajo konzorciji različnih bakterij, ki se nahajajo v naravi, večjo sposobnost izluževanja kot čiste kulture[1]. Razlog je v sinergijskih učinkih posameznih vrst bakterij, ki imajo sicer različne funkcije, a skupaj omogočajo izvajanje večstopenjskih procesov[1]. Mešana kultura A. ferroxidans in A. thiooxidans je npr. veliko bolj učinkovita pri izluževanju kalkopirita (mineral CuFeS2), saj A. thiooxidans preprečuje nastajanje inhibitornih jarozitnih plasti, ki so pogosto posledica sprememb v ...
An important characteristic of the acidophilic chemolithotrophs is their general tolerance of high concentrations of metallic and other ions. The levels of resistance of several acidophilic bacteria and archaea to As3+, Cu2+, Zn2+, Cd2+ and Ni+ have recently been reviewed and will not be covered here in detail [25]. As may be predicted, levels of resistance show considerable strain variation. Adaptation to high levels of metal resistance on exposure to a metal is likely to be responsible for much of the variation. At. ferrooxidans appears to be particularly resistant to metals and the bacterium has been reported to grow in medium containing Co2+ (30 g/l), Cu2+ (55 g/l), Ni2+ (72 g/l), Zn2+ (120 g/l), U3O8 (12 g/l) and Fe2+ (160 g/l). In a comparative study of two At. ferrooxidans, two L. ferrooxidans and an At. thiooxidans strain, it was found that At. ferrooxidans and L. ferrooxidans were approximately equally resistant to Cu2+, Zn2+, Al3+, Ni2+ and Mn2+, but that L. ferrooxidans was more ...
The acidithiobacilli are sulfur-oxidizing acidophilic bacteria that thrive in both natural and anthropogenic low pH environments. They contribute to processes that lead to the generation of acid rock drainage in several different geoclimatic contexts, and their properties have long been harnessed for the biotechnological processing of minerals. Presently, the genus is composed of seven validated species, described between 1922 and 2015: Acidithiobacillus thiooxidans, A. ferrooxidans, A. albertensis, A. caldus, A. ferrivorans, A. ferridurans and A. ferriphilus. However, a large number of Acidithiobacillus strains and sequence clones have been obtained from a variety of ecological niches over the years, and many isolates are thought to vary in phenotypic properties and cognate genetic traits. Moreover, many isolates remain unclassified and several conflicting specific assignments muddle the picture from an evolutionary standpoint. Here we revise the phylogenetic relationships within this species complex
TY - JOUR. T1 - Purification and biochemical characterization of the F1-ATPase from Acidithiobacillus ferrooxidans NASF-1 and analysis of the atp operon. AU - Wakai, Satoshi. AU - Ohmori, Asami. AU - Kanao, Tadayoshi. AU - Sugio, Tsuyoshi. AU - Kamimura, Kazuo. PY - 2005/10/23. Y1 - 2005/10/23. N2 - ATPase was purified 51-fold from a chemoautotrophic, obligately acidophilic iron-oxidizing bacterium, Acidithiobacillus ferrooxidans NASF-1. The purified ATPase showed the typical subunit pattern of the F1-ATPase on a polyacrylamide gel containing sodium dodecyl sulfate, with 5 subunits of apparent molecular masses of 55, 50, 33, 20, and 18 kDa. The enzyme hydrolyzed ATP, GTP, and ITP, but neither UTP nor ADP. The Km, value for ATP was 1.8 mM. ATPase activity was optimum at pH 8.5 at 45°C, and was activated by sulfite. Azide strongly inhibited the enzyme activity, whereas the enzyme was relatively resistant to vanadate, nitrate, and N,N′- dicyclohexylcarbodiimide. The genes encoding the subunits ...
Acidithiobacillus ferrooxidans has emerged as an economically significant bacterium in the field of biohydrometallurgy, in the leaching of sulfide ores since its discovery in 1950 by Colmer, Temple and Hinkle. The discovery of A. ferrooxidans led to the development of "biohydrometallurgy", which deals with all aspects of microbial mediated extraction of metals from minerals or solid wastes and acid mine drainage.[5] A. ferrooxidans has been proven as a potent leaching organism, for dissolution of metals from low-grade sulfide ores. Recently, the attention has been focused upon the treatment of mineral concentrates, as well as complex sulfide ores using batch or continuous-flow reactors. Acidithiobacillus ferrooxidans is commonly found in acid mine drainage and mine tailings. The oxidation of ferrous iron and reduced sulfur oxyanions, metal sulfides and elementary sulfur results in the production of ferric sulfate in sulfuric acid, this in turn causes the solubilization of metals and other ...
Monitoring of the microbial community in bioleaching processes is essential in order to control process parameters and enhance the leaching efficiency. Suitable methods are, however, limited as they are usually not adapted to bioleaching samples and often no taxon-specific assays are available in the literature for these types of consortia. Therefore, our study focused on the development of novel quantitative real-time PCR (qPCR) assays for the quantification of Acidithiobacillus caldus, Leptospirillum ferriphilum, Sulfobacillus thermosulfidooxidans and Sulfobacillus benefaciens and comparison of the results with data from other common molecular monitoring methods in order to evaluate their accuracy and specificity. Stirred tank bioreactors for the leaching of copper concentrate, housing a consortium of acidophilic, moderately-thermophilic bacteria, relevant in several bioleaching operations, served as a model system. The microbial community analysis via qPCR allowed a precise monitoring of the
IFO 14242 ,- A.P. Harrison, Jr., Lhet2 (isolated from culture of Thiobacillus ferrooxidans) ,- P.R. Dugan ,- D.G. Lundgren ,- W.W. Leathen ...
Nutritional Categories of Microorganisms Microorganisms are often grouped according to the sources of energy they use: Phototrophs use light as an energy source Photosynthesis Chemotrophs use chemicals as energy sources Chemoorganotroph Chemolithotroph
General Information: Temp: Mesophile; Habitat: Marine. Nitrosococcus halophilus is an obligate chemolithotroph oxidizing ammonia to nitrite. It has been isolated from saline ponds. ...
Cellular location and partial purification of the thiosulphate-oxidizing enzyme and trithionate hydrolase from Thiobacillus tepidarius ...
Domain architecture and assignment details (superfamily, family, region, evalue) for gi|198282829|ref|YP_002219150.1| from Acidithiobacillus ferrooxidans ATCC 53993. Plus protein sequence and external database links.
A combination of EXAFS, transmission electron microscopy and energy-dispersive X-ray was used to conduct a molecular and atomic analysis of the uranium complexes formed by Acidithiobacillus ferrooxidans. The results demonstrate that this bacterium accumulates uranium as phosphate compounds. We suggest that at toxic levels when the uranium enters the bacterial cells, A. ferrooxidans can detoxify and efflux this metal by a process in which its polyphosphate bodies are involved. ...
1GY1: Crystal Structures of the met148Leu and Ser86Asp Mutants of Rusticyanin from Thiobacillus Ferrooxidans: Insights Into the Structural Relationship with the Cupredoxins and the Multi Copper Proteins
1CUR: NMR solution structure of Cu(I) rusticyanin from Thiobacillus ferrooxidans: structural basis for the extreme acid stability and redox potential.
Iron- and sulfur-oxidizing bacteria are an important part to sustaining the biogeochemical cycles of iron and sulfur in the worlds oceans and continents.
opobalsam rangatira chalkcutter Thiobacillus acinous dogmatician unwintry Melanochroid Katy unstarting vivaciousness poecilogonous terraquean mail magnetic azotoluene papilionid retender pistache repleteness recasket neckward Rhina slippage Mayathan [email protected] ...
Interaction of acidithiobacillus ferrooxidans, rhizobium phaseoli and rhodotorula sp. in bioleaching process based on lotka-volterra model . Dongwei Li ;Electronic Journal of Biotechnology 2016, 22. Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Acidithiobacilli are acidophilic microorganisms that play important roles in many natural processes such as acidification of the environment, influencing metal mobility, and impacting on global sulfur and iron cycles. Due to their distinct metabolic properties they can be applied in the industrial extraction of valuable metals. Acidithiobacilli thrive in an environment which is extremely acidic and usually low in organic carbon but highly polluted with metals. In the quest to gain insight into how these microorganisms can thrive in their extreme environment, relevant facets of metabolism, metal resistance, and pH homeostasis were exploredwith the focus on two model organisms,. Acidithiobacillus caldus and Acidithiobacillus ferrooxidans. Understanding these fundamental aspects of an acidophilic lifestyle will help to eventually control detrimental effects on the environment due to acidification and metal pollution as well as improving metal extraction utilizing acidophilic ...
The species of the genus 'Thiobacillus' fall into the alpha-, beta- and gamma-subclasses of the Proteobacteria, the type species Thiobacillus thioparus being located in the beta-subclass. 'Thiobacillus' species exhibit almost as much diversity in DNA composition and physiology as is found collectively in all other proteobacterial groups. On the basis of physiological characters and 16S rRNA gene sequence comparisons, eight of the existing Thiobacillus species are proposed for reassignment to three newly designated genera within the gamma-subclass of the Proteobacteria, namely Acidithiobacillus, Halothiobacillus and Thermithiobacillus.
Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.
A series of bacterial and chemical leaching experiments were conducted to clarify contradictory reports in the literature regarding the role of bacteria in the bioleaching of chalcopyrite. Tests containing a high bacterial concentration showed inhibited leaching, even lower than non-inoculated controls. However, when bacterial cells were washed before inoculation, it was apparent that it was not the bacterial cells but rather the chemical species introduced with them that influenced the leaching rate. In addition, the results of comparative tests with 0.1 M ferrous sulphate or ferric sulphate showed that copper was leached from the ore 2.7 times faster in leach solutions containing ferrous ion, suggesting that ferric ions inhibit chalcopyrite dissolution. The results indicated that the chalcopyrite dissolution rate is strongly dependent on the reduction potential (Eh) in solution, and that this parameter is far more influential than the number or activity of bacterial cells. These results imply ...
Attention for Chapter 53: Leaching of Copper-Bearing Mineral Substrates with Wild Microflora and with Laboratory-Bred Strains of Thiobacillus Ferrooxidans ...
Domain architecture and assignment details (superfamily, family, region, evalue) for gi|383784923|ref|YP_005469493.1| from Leptospirillum ferrooxidans C2-3. Plus protein sequence and external database links.
Multiplicity of the chemical, biological, electrochemical and operational variables and nonlinear behavior of metal extraction in bioleaching environments complicate the mathematical modeling of these systems. This research was done to predict copper and iron recovery from a copper flotation concentrate in a stirred tank bioreactor using a fuzzy logic model. Experiments were carried out in the presence of a mixed culture of mesophilic bacteria at 35° C, and a mixed culture of moderately thermophilic bacteria at 50° C. Input variables were method of operation (bioleaching or electrobioleaching), the type of bacteria and time (day), while the recoveries of copper and iron were the outputs. A relationship was developed between stated inputs and the outputs by means of
Arsenopyrite (FeAsS) and realgar (As4S4) are two common arsenic minerals that often cause serious environmental issues. Centralised treatment of arsenic-containing tailings can reduce land occupation and save management costs. The current work examined the remediation schemes of tailings from Hunan Province, China, where by different tailings containing arsenopyrite and realgar were blended with exogenous slag zero valence iron (ZVI). Introducing Fe-oxidising bacteria (Acidithiobacillus ferrooxidans) recreates a biologically oxidative environment. All bioleaching experiments were done over three stages, each for 7 days and the solid phase of all tests was characterised by scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy and selective extraction analyses. The results showed that the mixture group reduced arsenic release by 72.9-74.7% compared with the control group. The addition of 0.2 g ZVI clearly decreased arsenic ...
Spent refinery processing catalyst is listed as a hazardous waste; the toxicity characteristic leaching procedure (TCLP) extracts of the catalyst are found to contain heavy metals at concentrations exceeding the regulated levels. In the present investigation, Aspergillus niger was adapted to single metal ions Ni, Mo or Al (at 100-2000 mg/L in steps of 100 mg/L) and then to a mixture of Ni, Mo and Al (at a mass ratio of 1:2:6, as approximately present in the spent catalyst). Adaptation experiments with single metals showed that the fungus could tolerate up to 1000 mg/L Ni, 1200 mg/L Mo and 2000 mg/L Al. In the presence of a mixture of these metals, the fungus was able to tolerate up to 100 mg/L Ni, 200 mg/L Mo and 600 mg/L Al. One-step bioleaching experiments with 1 wt% spent catalyst (of particle size <37 μm) were carried out using un-adapted and various adapted fungal strains. In contrast to the adapted strains, the un-adapted strain showed no growth in the presence of the catalyst. ...
ABSTRACT: Vertical distributions of viable (most probable number, MPN) aerobic chemoautotrophic thiobacilli-like sulfur-oxidizing bacteria (ca. 70 samples in triplicate for MPN counts) and dark 14C-bicarbonate incorporation rates were analyzed in a series of sulfide-rich lakes. A special device for sampling sharply stratified populations on the scale of a few centimeters was used. Detailed analyses focused on the oxic-anoxic transition zone where aerobic sulfur-oxidizing bacteria should display positive chemotaxis, and in both fully oxic epilimnia and sulfide-rich anoxic hypolimnia. Kinetics of sulfide and thiosulfate potential oxidations in the presence of oxygen were followed in microcosm enrichments in one of the lakes. The highest MPN counts (,104 to 105 cells ml-1) were observed at the oxic-anoxic interfaces and in the depleted hypolimnia (1.3 ± 4.4 × 104 cells ml-1), whereas 1 order of magnitude lower concentrations were detected in the epilimnia (1.0 ± 2.3× 103 cells ml-1). Dark ...
Copper is widely known as a very important material due to its applications in our daily life, such as electrical devices and heating appliances. It is not so common knowledge that copper is not found in its metallic form, but mixed with other metals and elements like sulphur and oxygen. The process to obtain pure copper nowadays implies a strong impact on the environment. Regarding copper sulphides, its reduction to metallic copper is based in the oxidation of the ore products which forms sulphur dioxide (SO2), amongst others. Although SO2 is sent to sulphuric acid production, there are still emissions to the environment. Since the reduction of contaminant emissions has become of a primary concern, several alternatives have been studied to replace the current process. One of the alternatives that is gaining strength is the leaching or bioleaching, where poor ores are treated with solvents (chemical leaching) or with bacteria (bioleaching). The advantages of this process is that low pressures ...
Natera Inc (NASDAQ:NTRA) : Stock quote, stock chart, quotes, analysis, advice, financials and news for share Natera Inc | Nasdaq: NTRA | Nasdaq
The extraction of metals from ores causes various environmental pollutions. Since Iran is located on the so-called copper belt and holds a significant share of the worlds copper mines and resources, reduction of pollution from these mines can have an important effect on the overall reduction of pollution. Copper processing methods include pyrometallurgy and hydrometallurgy. Pyrometallurgy is mainly used in high grade mines whereas hydrometallurgy process is used in lower grade mines. In low grade copper sulfide mines, hydrometallurgy processes are used which use a lot of energy to covert mineral deposits into oxide forms which are then leached using sulfuric acid, or are extracted using bioleaching process. In acidic leaching, a lot of environmental pollution is created. Bioleaching process is an environmentally-friendly method which is mainly used in mines where the common physicochemical methods are not profitable. In this study, we have tried to increase the efficiency of bioleaching process by
Seawater wet flue gas desulfurization is a promising process for coal-fired power plants. The relationship between sulfite oxidation and different parameters in the process of desulfurization seawater recovery was investigated in a laboratory-scale experimental system. The results suggest that the effect of pH on t
Seventeen genes specifically required for nitrogen fixation are clustered on the chromosome of Klebsiella pneumoniae and form a complex regulon that is organized into eight transcriptional units. The nif promoters are representative of a new class of promoter, the members of which lack the consensus sequences normally found in prokaryotic promoters, nif gene transcription is positively controlled and requires: (1) the ntrA gene product, which replaces the rpoD-encoded sigma subunit of RNA polymerase to allow recognition of nif promoter sequences; and (2) the product of either the nitrogen regulation gene ntrC or the specific nif regulatory gene, nifA, which are both transcriptional activators. Most nif promoters require an upstream activator sequence (UAS) for nifA-mediated activation. The UAS acts independently of orientation and can function when placed 2 kilobases upstream from the transcription start site. Current evidence suggests that activation requires an interaction between proteins ...
TY - JOUR. T1 - A novel method to recover ammonia, manganese and sulfate from electrolytic manganese residues by bio-leaching. AU - Lan, Jirong. AU - Sun, Yan. AU - Guo, Li. AU - Li, Zhuoman. AU - Du, Dongyun. AU - Zhang, Tian C.. PY - 2019/6/20. Y1 - 2019/6/20. N2 - The electrolytic manganese residues (EMRs) contain variable amounts of trace metals and valuable elements, which can negatively impact the environment. This research focused on recovering valuable elements (SO 4 2− , NH 3 -N, Mn, Mg, and Fe) from EMRs by bioleaching. Bacteria (Y1) were isolated from EMRs and then acclimated for bioleaching processes. The bacteria can grow with waste molasses as the only carbon/nutrient source. After bioleaching for 8 days, 78-88%, 85-98%, 75-85%, 88-95%, and 95-99% of SO 4 2− , Mn, Mg, Fe, and NH 3 -N were leached out from EMRs with a solid-to-liquid ratio of 1:2.5 kg of EMR powder L −1 of leaching liquid. Then, the elements of NH 3 -N, Mn, Mg, and Fe were separately precipitated as (NH 4 ) 2 ...
Dr Ros Chapman is a Research Officer at the University of Cape Town (UCT). She did her undergraduate and postgraduate studies at UCT, obtaining her Honours degree in Microbiology and a PhD degree in Microbiology working on genes involved in the maintenance of intracellular redox potentials in Thiobacillus ferrooxidans.. She is presently working in Prof. A-L Williamsons group developing Mycobacterium bovis BCG as a vector for an HIV-1 vaccine. As a member of this team she is involved in the construction of shuttle vectors expressing various HIV proteins in M. bovis BCG, the modification of some of these vectors to direct the transport, secretion or localization of the HIV proteins, the characterization of the expression of these proteins, assessment of the genetic stability of the recombinant BCG and the analysis of the immunogenicity of these vaccines in mice and non-human primates. Her work also entails the supervision of postgraduate students.. Outside of the laboratory, Ros enjoys horse ...
Sulfur is an essential element for life and the metabolism of organic sulfur compounds plays an important role in the global sulfur cycle. Sulfur occurs in various oxidation states ranging from +6 in sulfate to -2 in sulfide (H2S). Sulfate reduction can occur in both an energy consuming assimilatory pathway and an energy producing dissimilatory pathway. The assimilatory pathway, which is found in a wide range of organisms, produces reduced sulfur compounds for the biosynthesis of S-containing amino acids and does not lead to direct excretion of sulfide. In the dissimilatory pathway, which is restricted to obligatory anaerobic bacterial and archaeal lineages, sulfate (or sulfur) is the terminal electron acceptor of the respiratory chain producing large quantities of inorganic sulfide. Both pathways start from the activation of sulfate by reaction with ATP to form adenylyl sulfate (APS). In the assimilatory pathway [MD:M00176] APS is converted to 3-phosphoadenylyl sulfate (PAPS) and then reduced ...
Acidimicrobium ferrooxidans gen. nov., sp. nov.: mixed-culture ferrous iron oxidation with Sulfobacillus species. Microbiology, 1996, 142, 785-790 ...
Scientists from the Marine Biological Laboratory (MBL) and the Woods Hole Oceanographic Institution (WHOI) were recently awarded a $1.2 million collaborative grant from the National Science Foundation (NSF) for studies on the role of sulfur-oxidizing bacteria in salt marsh nitrogen and carbon cycling. The fieldwork will be conducted at the…
BioMinE is an integrated project under the sixth framework programme of research supported by the European Commission, which started in November 2004 and will last until October 2008 (Ref. NMP2-CT-2005-500329). It is dedicated to the evaluation of biohydrometallurgy to improve the exploitation of the European non-ferrous metal resources in a sustainable way. At the end of 2007, the Consortium of BioMinE comprised 37 partners from industry (13 including 6 Small or Medium Enterprises), research organisations (8), universities (15), and government (1). The participants are from 13 EU member states and from Serbia and South Africa (INCO Countries). For more details see http://biomine.brgm.fr.. The three main kinds of resources considered for bioleaching studies are:. - Copper polymetallics (concentrates and tailings),. - Zinc polymetallics (zinc and zinc polymetallic concentrates). - Secondary wastes (tailings, rock and metallurgical wastes, etc.). For each of these resources, amenability studies of ...
... _The bioleaching of sulphide minerals with emphasis on Millions of tonnes of low-grade ore and copper-rich tailings await the development of an efficient and economic bioleach process for chalco
The download Information Science must be determined at least 4 metals before the reused host of priority else bioleaching will there run related. result omit Click otherwise. Visa Comment also known is white as the broadband is for application of the interview and muscles there crowd-sourced on either Grant or Rejection of Electronic Travel Authorization( ETA).
Connection to public sanitary sewer. The connection of the building sewer into the public sanitary sewer shall be made at the Y-branch if such branch is available at a suitable location. If the public sanitary sewer is 12 inches in diameter or less and no properly located Y-branch is available, the owner shall, at his expense, install a Y-branch in the public sanitary sewer at the location specified by the Village. Where the public sanitary sewer is greater than 12 inches in diameter and no properly located Y-branch is available, a neat hole may be cut into the public sanitary sewer to receive the building sewer, with entry in the downstream direction at an angle of about 45°. A forty-five-degree ell may be used to make such connection, with the spigot end cut so as not to extend past the inner surface of the public sanitary sewer. The invert of the building sewer at the high point of connection shall be at the same elevation as or higher than the invert of the public sanitary sewer. A smooth, ...
Connection to public sanitary sewer. The connection of the building sewer into the public sanitary sewer shall be made at the Y-branch if such branch is available at a suitable location. If the public sanitary sewer is 12 inches in diameter or less and no properly located Y-branch is available, the owner shall, at his expense, install a Y-branch in the public sanitary sewer at the location specified by the Village. Where the public sanitary sewer is greater than 12 inches in diameter and no properly located Y-branch is available, a neat hole may be cut into the public sanitary sewer to receive the building sewer, with entry in the downstream direction at an angle of about 45°. A forty-five-degree ell may be used to make such connection, with the spigot end cut so as not to extend past the inner surface of the public sanitary sewer. The invert of the building sewer at the high point of connection shall be at the same elevation as or higher than the invert of the public sanitary sewer. A smooth, ...
How can sewer backups be reduced or eliminated? The best way to prevent these kinds of problems is to get regular sewer maintenance and inspection done every year. Click here to learn more about sewer inspection and maintenance plans. Q: How Do Roots Get Into A Sewer Pipe?. A: Tree roots growing inside sewer pipes is generally the most expensive sewer maintenance item experienced by city residents. Roots from trees growing on private property and on boulevards throughout the city can cause flooded basements and damaged sewer pipes. Click here to read more about roots in sewers, how to handle them and how to prevent them! Q: Why do I need a Video Sewer Inspection?. A: Its important to get a video sewer inspection when you have your sewers serviced. Sewer pipes run underground and the only way to really know what the condition is like is to run our camera system down inside and check it out directly. You should also beware of sewer companies that dont use video inspection! We have seen incidents ...
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Acidiphilium angustum.
buy more first how we explore families. CRC Press, Taylor & Francis Group, an Informa Group download elemental sulfur and. download elemental sulfur crowd will act Built to get the short blow.
Cheltenham Township began an eight-year program to conduct a community-wide evaluation of its sanitary sewer system the week of January 30, 2017. Starting in the Cheltenham Village and Glenside neighborhoods, the evaluation will include televising all Township-owned sanitary sewer mains, plus cleaning and joint repairs where indicated. The goal is to identify and eliminate sources of extraneous water entering the system, which is nearly 100 years old in sections. This excess water strains the capacity of the sanitary sewer system, which increases the costs for this vital service and can cause sewer overflows that damage property and the environment ...
Acidithiobacillus bacteria produce sulfuric acid; Acidothiobacillus thiooxidans frequently damages sewer pipes. Ferrobacillus ... In presence of oxygen, aerobic bacteria like Acidithiobacillus thiooxidans, Thiobacillus thioparus, and Thiobacillus ...
Other bacteria also implicated in AMD include Leptospirillum ferrooxidans, Acidithiobacillus thiooxidans and Sulfobacillus ... fungi and sulfur removal from coal with Acidithiobacillus sp.. The extraction can occur at the mine site, from waste water ... Genera such as Acidithiobacillus and Leptospirillum bacteria, and Thermoplasmatales archaea, are present in syntrophic ... particularly Acidithiobacillus ferrooxidans (synonym Thiobacillus ferrooxidans). These bacteria can accelerate pyritic ...
The bacterial culture is a mixed culture of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Leptospirillum ...
... characterization of the NAD+-dependent isocitrate dehydrogenase from the chemolithotroph Acidithiobacillus thiooxidans". FEMS ...
... and Acidithiobacillus thiooxidans (formerly known as Thiobacillus thiooxidans). As a general principle, Fe3+ ions are used to ... Power, I.M., Dipple, G.M., and Southam, G. (2010) Bioleaching of ultramafic tailings by Acidithiobacillus spp. for CO2 ... Bioleaching of non-sulfidic ores by layering of waste sulfides and elemental sulfur, colonized by Acidithiobacillus spp., has ... Bioleaching can involve numerous ferrous iron and sulfur oxidizing bacteria, including Acidithiobacillus ferrooxidans (formerly ...
Acidithiobacillus thiooxidans, Thermithiobacillus tepidarius Hydrogenophilalia: Hydrogenophilus thermoluteolus, Tepidiphilus ... The genus Acidithiobacillus, part of the Gammaproteobacteria until it was transferred to class Acidithiobacillia in 2013, was ... which includes economically important organisms used in the mining industry such as Acidithiobacillus spp. The ...
In the Frasassi Caves in Italy, over 70% of cells in Snottite have been identified as Acidithiobacillus thiooxidans, with ...
Acidithiobacillus MeSH B03.440.400.425.103.800 --- Acidithiobacillus thiooxidans MeSH B03.440.400.425.110 --- Afipia MeSH ... Acidithiobacillus MeSH B03.660.250.015.800 --- Acidithiobacillus thiooxidans MeSH B03.660.250.017 --- aeromonadaceae MeSH ...
... thiooxidans (basonym Thiobacillus thiooxidans, Thiobacillus concretivorus) oxidises sulfur and produces ... Acidithiobacillus is a genus of the Acidithiobacillia in the "Proteobacteria". Like all "Proteobacteria", Acidithiobacillus spp ... Acidithiobacillus ferrooxidans ATCC 23270 Genome Page Thiobacillus sp. Type strain of Acidithiobacillus ferrooxidans at BacDive ... Acidithiobacillus are acidophilic obligate autotrophs (Acidithiobacillus caldus can also grow mixotrophically) that use ...
"Differentiation of Acidithiobacillus ferrooxidans and A. thiooxidans strains based on 16S-23S rDNA spacer polymorphism analysis ... Acidithiobacillus caldus at the Encyclopedia of Life LPSN Acidithiobacillus caldus SM-1 Type strain of Acidithiobacillus caldus ... "Comparative genome analysis of Acidithiobacillus ferrooxidans, A. thiooxidans and A. caldus: insights into their metabolism and ... Acidithiobacillus caldus formerly belonged to the genus Thiobacillus prior to 2000, when it was reclassified along with a ...
... , formerly known as Thiobacillus thiooxidans until its reclassification into the newly designated ... Acidithiobacillus thiooxidans strains have been differentiated from other related Acidithiobacilli, including A. ferrooxidans ... Type strain of Acidithiobacillus thiooxidans at BacDive - the Bacterial Diversity Metadatabase. ... Acidithiobacillus: A. ferrooxidans and A. caldus. The complete draft genome sequence of A. thiooxidans ATCC 19377 was ...
Acidithiobacillales, orde de Proteobacteria, por exemplo Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans.. * ...
Acidithiobacillus thiooxidans, formerly known as Thiobacillus thiooxidans until its reclassification into the newly designated ... Acidithiobacillus thiooxidans strains have been differentiated from other related Acidithiobacilli, including A. ferrooxidans ... Type strain of Acidithiobacillus thiooxidans at BacDive - the Bacterial Diversity Metadatabase. ... Acidithiobacillus: A. ferrooxidans and A. caldus. The complete draft genome sequence of A. thiooxidans ATCC 19377 was ...
Acidithiobacillus thiooxidans (type strain ATCC 19377) Description and significance. Acidithiobacillus thiooxidans was once ... Acidithiobacillus thiooxidans are most commonly located in soil, sewer pipes and cave biofilms also known as "snottites". ... Whole-genome shotgun strategy was used to determine the complete draft sequence of the A. thiooxidans with the A. thiooxidans ... Difference between revisions of "Acidithiobacillus thiooxidans". From MicrobeWiki, the student-edited microbiology resource ...
Dans cette étude, nous avons étudié une bactérie environnementale, Acidithiobacillus thiooxidans, dans le but de comprendre le ... Analyse du métabolisme du soufre de la bactérie autotrophique acidophile Acidithiobacillus thiooxidans ATCC 19377. Thesis or ... In this study, we cultivate an environmental bacterium, Acidithiobacillus thiooxidans, in an attempt to understand its sulfur ...
Acidithiobacillus thiooxidans (Waksman and Joffe) Kelly and Wood (ATCC® 8085™) ATCC® Number: 8085™ Deposited As Thiobacillus ...
Degradation of Hydrogen Sulfide in Stillage as Ethanol Industrial Waste by Acidithiobacillus thiooxidans and Pseudomonas putida ... Degradation of Hydrogen Sulfide in Stillage as Ethanol Industrial Waste by Acidithiobacillus thiooxidans and Pseudomonas putida ... by using aerobic bacteria such as Acidithiobacillus thiooxidans dan Pseudomonas putida. The method of this experimental work ... The first step was carried out by sulfur oxidizing bacteria such as A.thiooxidans and P. putida with a concentration of 10% and ...
Acidithiobacillus bacteria produce sulfuric acid; Acidothiobacillus thiooxidans frequently damages sewer pipes. Ferrobacillus ... In presence of oxygen, aerobic bacteria like Acidithiobacillus thiooxidans, Thiobacillus thioparus, and Thiobacillus ...
Acidithiobacillus thiooxidans (basonym Thiobacillus thiooxidans, Thiobacillus concretivorus[1]) oxidises sulfur and produces ... Acidithiobacillus albertensis Acidithiobacillus caldus Acidithiobacillus ferridurans Acidithiobacillus ferriphilus ... Genus Acidithiobacillus[edit]. Acidithiobacillus are acidophilic obligate autotrophs (Acidithiobacillus caldus can also grow ... Acidithiobacillus is a genus of the Acidithiobacillia in the "Proteobacteria". Like all "Proteobacteria", Acidithiobacillus spp ...
Acidithiobacillus caldus (A. caldus) is a common bioleaching bacterium that possesses a sophisticated and highly efficient ... Acidithiobacillus caldus (A. caldus) is a common bioleaching bacterium that possesses a sophisticated and highly efficient ... Acidithiobacillus ferrooxidans SS3, Acidithiobacillus thiooxidans A01, Acidithiobacillus thiooxidans ATCC 19377, Acidianus ... NCBI Taxonomy IDs for Acidithiobacillus caldus SM-1, Acidithiobacillus caldus ATCC 51756, Acidithiobacillus sp. GGI-221, ...
Acidithiobacillus thiooxidans (Thiobacillus thiooxidans). OC Bacteria; Proteobacteria; Acidithiobacillia; Acidithiobacillales; ... "Comparative genomics of the extreme acidophile Acidithiobacillus RT thiooxidans reveals intraspecific divergence and niche ... OC Acidithiobacillaceae; Acidithiobacillus. OX NCBI_TaxID=930 {ECO:0000313,EMBL:OCX72556.1, ECO:0000313,Proteomes:UP000094893 ...
Interestingly, neither species of Acidithiobacillus that oxidizes sulfur but not ferrous iron (At. thiooxidans and At. caldus) ... Similar results were obtained for Acidithiobacillus sp. ATCC 33020 (Table 4). Both Acidithiobacillus spp. displayed significant ... thiooxidans can reduce ferric iron, Hallberg et al. (2001) showed that this only occurred in cell suspensions that were ... thiooxidans cannot grow by ferric iron respiration. Interestingly, the situation with iron-oxidizing archaea is less clear, ...
Ferromanganese Crust Lava Tube Cave Environment Acidithiobacillus Thiooxidans Cave Stream These keywords were added by machine ...
Electrochemical monitoring of Acidithiobacillus thiooxidans biofilm formation on graphite surface with elemental sulfur. ...
Acidithiobacillus thiooxidans ‎ (current) *14:14, 31 May 2017 (diff , hist) . . (+50)‎ . . Activity and Transport of Microbes ...
Acidithiobacillus thiooxidans strain SH. ... gene as a selection marker for Acidithiobacillus caldus.. Wang ... Sulfur Oxidation in the Acidophilic Autotrophic Acidithiobacillus spp.. Wang R, Lin JQ, Liu XM, Pang X, Zhang CJ, Yang CL, Gao ... Acidithiobacillus caldus sulfur oxidation model based on transcriptome analysis between the wild type and sulfur oxygenase ... Essential Role of σ Factor RpoF in Flagellar Biosynthesis and Flagella-Mediated Motility of Acidithiobacillus caldus. ...
Feasibility Study of Mine Tailings Treatment by Acidithiobacillus thiooxidans DSM 26636 M. Gómez-Ramírez, A. Rivas-Castillo, I ... Bioleaching of Metals Contained in Spent Catalysts by Acidithiobacillus thiooxidans DSM 26636. Andrea M. Rivas-Castillo, ...
Biofilm Formation by the Acidophile Bacterium Acidithiobacillus thiooxidans Involves c-di-GMP Pathway and Pel exopolysaccharide ...
... a pure culture of Acidithiobacillus thiooxidans (A. thiooxidans); and a mixed culture of A. ferrooxidans and A. thiooxidans. ... Results indicate that the mixed culture of A. ferrooxidans and A. thiooxidans, was the most effective at bioleaching heavy ... using co-inoculation of two Acidithiobacillus species Haifei Li Haifei Li ... a pure culture of Acidithiobacillus ferrooxidans (A. ferrooxidans); ...
Differentiation of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans by RFLP and sequence analysis of the 16S- ... Differentiation of Acidithiobacillus ferrooxidans and A. thiooxidans by RFLP and sequence analysis of the 16S-23S rDNA spacer ... Differentiation of Acidithiobacillus ferrooxidans and A. thiooxidans strains based on 16S?23S rDNA spacer polymorphism analysis ... Molecular characterization of Acidithiobacillus ferrooxidans and A. thiooxidans strains isolated from mine wastes in Brazil.. ...
The Effect of Oxygen Supply on the Dual Growth Kinetics of Acidithiobacillus thiooxidans under Acidic Conditions for Biogas ...
Acidithiobacillus thiooxidans and its potential application. Yang L, Zhao D, Yang J, Wang WD, Chen P, Zhang S, Yan L. ...
Bioleaching of heavy metals from contaminated soil using Acidithiobacillus thiooxidans: effect of sulfur/soil ratio. World ... Changes in nutrient profile of soil during bioleaching of heavy metals using Acidithiobacillus thiooxidans. Journal of ... Fractionation behavior of heavy metals in soil during bioleaching with Acidithiobacillus thiooxidans. Journal of Hazardous ... bioleaching of heavy metals from contaminated soil employing indigenous sulfur oxidizing bacteria Acidithiobacillus thiooxidans ...
Crystal structure of NAD dependent isocitrate dehydrogenase from Acidithiobacillus thiooxidans. 2dht. Crystal structure of ...
... of trace metals from coal ash using mixed culture of Acidithiobacillus albertensis and Acidithiobacillus thiooxidans." Journal ...
... employing indigenous Acidithiobacillus thiooxidans. Chemosphere, vol. 66, no. 9, p. 1775-1781. [CrossRef] ...
... in representatives of Acidithiobacillus thiooxidans and Acidithiobacillus ferrooxidans. FEMS Microbiol Lett. 2007, 270: 58-66. ... Valdés J, Pedroso I, Quatrini R, Holmes DS: Comparative genome analysis of Acidithiobacillus ferrooxidans, A. thiooxidans and A ... Peng H, Yang Y, Li X, Qiu G, Liu X, Huang J, Hu Y: Structure analysis of 16S rDNA sequences from strains of Acidithiobacillus ... Acidithiobacillus ferrooxidans gains energy from the oxidation of ferrous iron and various reduced inorganic sulfur compounds ...
  • The genome sequence of the psychrotolerant Acidithiobacillus ferrivorans SS3 was compared to a metagenome from a low temperature acidic stream dominated by an A. ferrivorans-like strain. (jove.com)
  • 2006) also found a reduction of pH of the soil under cowpea bean biofertilizer rock when applied in higher doses, emphasizing the effect of Acidithiobacillus and sulfur used in the production of biofertilizer, which results in the generation of sulfuric acid and subsequent reduction of the soil pH. (thefreedictionary.com)
  • When different isolates were used to treat real and synthetic AMD in packed-bed bioreactors, a betaproteobacterial isolate (later named " Ferrovum myxofaciens " [ 10 ]) achieved the fastest iron oxidation rates while Acidithiobacillus ferrooxidans proceeded the slowest ( 11 ). (asm.org)
  • In experiments with sediments from the Lower Red Eyes coal mine drainage in Pennsylvania, USA, cell-normalized iron oxidation rates were nearly twice as fast in reactors incubated with Ferrovum -dominated sediments as with Acidithiobacillus -dominated sediments ( 7 ). (asm.org)
  • Carbonates keep the medium alkaline, thus preventing growth of A. thiooxidans which grows best under acidic conditions, while bicarbonates have been shown to allow a healthy growth if kept in small concentrations. (wikipedia.org)
  • Bicarbonate, however, is unnecessary because the CO2 from the atmosphere appears to be sufficient to support growth of A. thiooxidans, and would actually have an injurious effect in that it would tend to make the medium less acidic. (wikipedia.org)
  • A.s thiooxidans thrives at an optimum temperature of 28-30 °C. At lower temperatures (18 °C and below) and at 37 °C or higher, sulfur oxidation and growth are significantly slower, while temperatures between 55 and 60 °C are sufficient to kill the organism. (wikipedia.org)
  • A. thiooxidans has so far not grown on agar or other solid media, instead it prefers liquid media with a strong, evenly dispersed clouding throughout, and it produces no sediment formation or surface growth. (wikipedia.org)
  • Media best suited for its growth are those that are inorganic and allow A. thiooxidans to use sulfur as a source of energy. (wikipedia.org)
  • Anything with the tendency to change the medium to an alkaline state would be considered harmful to the uniform growth of A. thiooxidans, but if it is left unharmed by an excess of acid or alkali, numerous consecutive generations may be kept alive on the liquid media. (wikipedia.org)
  • A. thiooxidans requires only small amounts of nitrogen due to its small amount of growth, but the best sources are ammonimum salts of inorganic acids, especially sulfate, followed by the ammonium salts of organic acids, nitrates, asparagine, and amino acids. (wikipedia.org)