Acidithiobacillus: A genus of gram-negative rod-shaped bacteria in the class GAMMAPROTEOBACTERIA. They are obligately acidophilic and aerobic, using reduced SULFUR COMPOUNDS to support AUTOTROPHIC GROWTH.Acidithiobacillus thiooxidans: A strictly autotrophic species of bacteria that oxidizes sulfur and thiosulfate to sulfuric acid. It was formerly called Thiobacillus thiooxidans.Tetrathionic Acid: A sulfuric acid dimer, formed by disulfide linkage. This compound has been used to prolong coagulation time and as an antidote in cyanide poisoning.Sulfur: An element that is a member of the chalcogen family. It has an atomic symbol S, atomic number 16, and atomic weight [32.059; 32.076]. It is found in the amino acids cysteine and methionine.Gammaproteobacteria: A group of the proteobacteria comprised of facultatively anaerobic and fermentative gram-negative bacteria.Sulfur Compounds: Inorganic or organic compounds that contain sulfur as an integral part of the molecule.Thiobacillus: A genus of gram-negative, rod-shaped bacteria that derives energy from the oxidation of one or more reduced sulfur compounds. Many former species have been reclassified to other classes of PROTEOBACTERIA.Sulfides: Chemical groups containing the covalent sulfur bonds -S-. The sulfur atom can be bound to inorganic or organic moieties.MiningTungsten Compounds: Inorganic compounds that contain tungsten as an integral part of the molecule.Acetobacteraceae: A family of gram-negative aerobic bacteria consisting of ellipsoidal to rod-shaped cells that occur singly, in pairs, or in chains.Ferrous Compounds: Inorganic or organic compounds that contain divalent iron.Organomercury Compounds: Organic compounds which contain mercury as an integral part of the molecule.Azurin: A bacterial protein from Pseudomonas, Bordetella, or Alcaligenes which operates as an electron transfer unit associated with the cytochrome chain. The protein has a molecular weight of approximately 16,000, contains a single copper atom, is intensively blue, and has a fluorescence emission band centered at 308nm.Iron: A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.Oxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).Iron Compounds: Organic and inorganic compounds that contain iron as an integral part of the molecule.Proteobacteria: A phylum of bacteria consisting of the purple bacteria and their relatives which form a branch of the eubacterial tree. This group of predominantly gram-negative bacteria is classified based on homology of equivalent nucleotide sequences of 16S ribosomal RNA or by hybridization of ribosomal RNA or DNA with 16S and 23S ribosomal RNA.Mercury: A silver metallic element that exists as a liquid at room temperature. It has the atomic symbol Hg (from hydrargyrum, liquid silver), atomic number 80, and atomic weight 200.59. Mercury is used in many industrial applications and its salts have been employed therapeutically as purgatives, antisyphilitics, disinfectants, and astringents. It can be absorbed through the skin and mucous membranes which leads to MERCURY POISONING. Because of its toxicity, the clinical use of mercury and mercurials is diminishing.Sulfites: Inorganic salts of sulfurous acid.Hydrogen Sulfide: A flammable, poisonous gas with a characteristic odor of rotten eggs. It is used in the manufacture of chemicals, in metallurgy, and as an analytical reagent. (From Merck Index, 11th ed)Pulpotomy: Dental procedure in which part of the pulp chamber is removed from the crown of a tooth.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Sulfuric Acids: Inorganic and organic derivatives of sulfuric acid (H2SO4). The salts and esters of sulfuric acid are known as SULFATES and SULFURIC ACID ESTERS respectively.Thermoplasmales: An order of aerobic, thermophilic archaea, in the kingdom EURYARCHAEOTA, characterized by the absence of a cell wall. Two genera have been described: THERMOPLASMA and Picrophilus.3-Mercaptopropionic Acid: An inhibitor of glutamate decarboxylase. It decreases the GAMMA-AMINOBUTYRIC ACID concentration in the brain, thereby causing convulsions.Sulfonium Compounds: Sulfur compounds in which the sulfur atom is attached to three organic radicals and an electronegative element or radical.Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing): An enzyme that catalyzes the synthesis of fructose-6-phosphate plus GLUTAMINE from GLUTAMATE plus glucosamine-6-phosphate.Transaminases: A subclass of enzymes of the transferase class that catalyze the transfer of an amino group from a donor (generally an amino acid) to an acceptor (generally a 2-keto acid). Most of these enzymes are pyridoxyl phosphate proteins. (Dorland, 28th ed) EC 2.6.1.Prephenate Dehydrogenase: An enzyme that catalyzes the conversion of prephenate to p-hydroxyphenylpyruvate in the presence of NAD. In the enteric bacteria, this enzyme also possesses chorismate mutase activity, thereby catalyzing the first two steps in the biosynthesis of tyrosine. EC 1.3.1.12.Lakes: Inland bodies of still or slowly moving FRESH WATER or salt water, larger than a pond, and supplied by RIVERS and streams.Uranyl Nitrate: Bis(nitrato-O)dioxouranium. A compound used in photography and the porcelain industry. It causes severe renal insufficiency and renal tubular necrosis in mammals and is an effective lymphocyte mitogen.Uranium: Uranium. A radioactive element of the actinide series of metals. It has an atomic symbol U, atomic number 92, and atomic weight 238.03. U-235 is used as the fissionable fuel in nuclear weapons and as fuel in nuclear power reactors.SvalbardHydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Electron Probe Microanalysis: Identification and measurement of ELEMENTS and their location based on the fact that X-RAYS emitted by an element excited by an electron beam have a wavelength characteristic of that element and an intensity related to its concentration. It is performed with an electron microscope fitted with an x-ray spectrometer, in scanning or transmission mode.Spectrometry, X-Ray Emission: The spectrometric analysis of fluorescent X-RAYS, i.e. X-rays emitted after bombarding matter with high energy particles such as PROTONS; ELECTRONS; or higher energy X-rays. Identification of ELEMENTS by this technique is based on the specific type of X-rays that are emitted which are characteristic of the specific elements in the material being analyzed. The characteristic X-rays are distinguished and/or quantified by either wavelength dispersive or energy dispersive methods.Rhodotorula: A red yeast-like mitosporic fungal genus generally regarded as nonpathogenic. It is cultured from numerous sources in human patients.Rhizobium phaseoli: A species of gram-negative bacteria functioning as a nitrogen inoculum for dry beans, especially species in the genus PHASEOLUS.Rhizobium: A genus of gram-negative, aerobic, rod-shaped bacteria that activate PLANT ROOT NODULATION in leguminous plants. Members of this genus are nitrogen-fixing and common soil inhabitants.Halothiobacillus: A genus of gram-negative, chemolithoautotrophic bacteria in the family Halothiobacillaceae. Several of its species were reclassified to this genus from THIOBACILLUS.RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.Sulfur Oxides: Inorganic oxides of sulfur.DNA, Ribosomal: DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.Cellular Phone: Analog or digital communications device in which the user has a wireless connection from a telephone to a nearby transmitter. It is termed cellular because the service area is divided into multiple "cells." As the user moves from one cell area to another, the call is transferred to the local transmitter.MichiganMobile Applications: Computer programs or software installed on mobile electronic devices which support a wide range of functions and uses which include television, telephone, video, music, word processing, and Internet service.Computers, Handheld: A type of MICROCOMPUTER, sometimes called a personal digital assistant, that is very small and portable and fitting in a hand. They are convenient to use in clinical and other field situations for quick data management. They usually require docking with MICROCOMPUTERS for updates.Awards and PrizesTeaching: The educational process of instructing.Physiology: The biological science concerned with the life-supporting properties, functions, and processes of living organisms or their parts.

The structure of Acidithiobacillus ferrooxidans c(4)-cytochrome: a model for complex-induced electron transfer tuning. (1/101)

The study of electron transfer between the copper protein rusticyanin (RCy) and the c(4)-cytochrome CYC(41) of the acidophilic bacterium Acidithiobacillus ferrooxidans has evidenced a remarkable decrease of RCy's redox potential upon complex formation. The structure of the CYC(41) obtained at 2.2 A resolution highlighted a specific glutamate residue (E121) involved in zinc binding as potentially playing a central role in this effect, required for the electron transfer to occur. EPR and stopped-flow experiments confirmed the strong inhibitory effect of divalent cations on CYC(41):RCy complex formation. A docking analysis of the CYC(41) and RCy structure allows us to propose a detailed model for the complex-induced tuning of electron transfer in agreement with our experimental data, which could be representative of other copper proteins involved in electron transfer.  (+info)

Respiratory isozyme, two types of rusticyanin of Acidithiobacillus ferrooxidans. (2/101)

Among the members of the copper protein superfamily, the type I enzyme rusticyanin, which is found as an electron carrier in the oxidative respiratory chain of Acidithiobacillus ferrooxidans, is the only one to have both a high redox potential and acid stability. Here we report that two forms of the rusticyanin gene (rus) are present in the genomes of some strains of A. ferrooxidans. The more common form of rus (type-A) was found to be present in all six strains studied, including those harboring only a single copy of the gene. In addition a less common form (type-B) occurred in strains harboring multiple copies of the gene. The two genes were expressed as rusticyanin isozymes with differing surface charges due to differences in their amino acid composition. Still, the copper coordination sites were completely conserved, thereby maintaining the high redox potential necessary for an electron carrier.  (+info)

Immobilization of arsenite and ferric iron by Acidithiobacillus ferrooxidans and its relevance to acid mine drainage. (3/101)

Weathering of the As-rich pyrite-rich tailings of the abandoned mining site of Carnoules (southeastern France) results in the formation of acid waters heavily loaded with arsenic. Dissolved arsenic present in the seepage waters precipitates within a few meters from the bottom of the tailing dam in the presence of microorganisms. An Acidithiobacillus ferrooxidans strain, referred to as CC1, was isolated from the effluents. This strain was able to remove arsenic from a defined synthetic medium only when grown on ferrous iron. This A. ferrooxidans strain did not oxidize arsenite to arsenate directly or indirectly. Strain CC1 precipitated arsenic unexpectedly as arsenite but not arsenate, with ferric iron produced by its energy metabolism. Furthermore, arsenite was almost not found adsorbed on jarosite but associated with a poorly ordered schwertmannite. Arsenate is known to efficiently precipitate with ferric iron and sulfate in the form of more or less ordered schwertmannite, depending on the sulfur-to-arsenic ratio. Our data demonstrate that the coprecipitation of arsenite with schwertmannite also appears as a potential mechanism of arsenite removal in heavily contaminated acid waters. The removal of arsenite by coprecipitation with ferric iron appears to be a common property of the A. ferrooxidans species, as such a feature was observed with one private and three collection strains, one of which was the type strain.  (+info)

Coevolution of an aminoacyl-tRNA synthetase with its tRNA substrates. (4/101)

Glutamyl-tRNA synthetases (GluRSs) occur in two types, the discriminating and the nondiscriminating enzymes. They differ in their choice of substrates and use either tRNAGlu or both tRNAGlu and tRNAGln. Although most organisms encode only one GluRS, a number of bacteria encode two different GluRS proteins; yet, the tRNA specificity of these enzymes and the reason for such gene duplications are unknown. A database search revealed duplicated GluRS genes in >20 bacterial species, suggesting that this phenomenon is not unusual in the bacterial domain. To determine the tRNA preferences of GluRS, we chose the duplicated enzyme sets from Helicobacter pylori and Acidithiobacillus ferrooxidans. H. pylori contains one tRNAGlu and one tRNAGln species, whereas A. ferrooxidans possesses two of each. We show that the duplicated GluRS proteins are enzyme pairs with complementary tRNA specificities. The H. pylori GluRS1 acylated only tRNAGlu, whereas GluRS2 was specific solely for tRNAGln. The A. ferrooxidans GluRS2 preferentially charged tRNA(UUG)(Gln). Conversely, A. ferrooxidans GluRS1 glutamylated both tRNAGlu isoacceptors and the tRNA(CUG)(Gln) species. These three tRNA species have two structural elements in common, the augmented D-helix and a deletion of nucleotide 47. It appears that the discriminating or nondiscriminating natures of different GluRS enzymes have been derived by the coevolution of protein and tRNA structure. The coexistence of the two GluRS enzymes in one organism may lay the groundwork for the acquisition of the canonical glutaminyl-tRNA synthetase by lateral gene transfer from eukaryotes.  (+info)

Enzymatic synthesis of lipid A molecules with four amide-linked acyl chains. LpxA acyltransferases selective for an analog of UDP-N-acetylglucosamine in which an amine replaces the 3"-hydroxyl group. (5/101)

LpxA of Escherichia coli catalyzes the acylation of the glucosamine 3-OH group of UDP-GlcNAc, using R-3-hydroxymyristoyl-acyl carrier protein (ACP) as the donor substrate. We now demonstrate that LpxA in cell extracts of Mesorhizobium loti and Leptospira interrogans, which synthesize lipid A molecules containing 2,3-diamino-2,3-dideoxy-d-glucopyranose (GlcN3N) units in place of glucosamine, do not acylate UDP-GlcNAc. Instead, these LpxA acyltransferases require a UDP-Glc-NAc derivative (designated UDP 2-acetamido-3-amino-2,3-dideoxy-alpha-d-glucopyranose or UDP-GlcNAc3N), characterized in the preceding paper, in which an amine replaces the glucosamine 3-OH group. L. interrogans LpxA furthermore displays absolute selectivity for 3-hydroxylauroyl-ACP as the donor, whereas M. loti LpxA functions almost equally well with 10-, 12-, and 14-carbon 3-hydroxyacyl-ACPs. The substrate selectivity of L. interrogans LpxA is consistent with the structure of L. interrogans lipid A. The mechanism of L. interrogans LpxA appears to be similar to that of E. coli LpxA, given that the essential His(125) residue of E. coli LpxA is conserved and is also required for acyltransferase activity in L. interrogans. Acidithiobacillus ferrooxidans (an organism that makes lipid A molecules containing both GlcN and GlcN3N) has an ortholog of LpxA that is selective for UDP-GlcNAc3N, but the enzyme also catalyzes the acylation of UDP-GlcNAc at a slow rate. E. coli LpxA acylates UDP-GlcNAc and UDP-GlcNAc3N at comparable rates in vitro. However, UDP-GlcNAc3N is not synthesized in vivo, because E. coli lacks gnnA and gnnB. When the latter are supplied together with A. ferrooxidans lpxA, E. coli incorporates a significant amount of GlcN3N into its lipid A.  (+info)

Oxidation and transamination of the 3"-position of UDP-N-acetylglucosamine by enzymes from Acidithiobacillus ferrooxidans. Role in the formation of lipid a molecules with four amide-linked acyl chains. (6/101)

Lipid A, a major component of the outer membranes of Escherichia coli and other Gram-negative bacteria, is usually constructed around a beta-1',6-linked glucosamine disaccharide backbone. However, in organisms like Acidithiobacillus ferrooxidans, Leptospira interrogans, Mesorhizobium loti, and Legionella pneumophila, one or both glucosamine residues are replaced with the sugar 2,3-diamino-2,3-dideoxy-d-glucopyranose. We now report the identification of two proteins, designated GnnA and GnnB, involved in the formation of the 2,3-diamino-2,3-dideoxy-d-glucopyranose moiety. The genes encoding these proteins were recognized because of their location between lpxA and lpxB in A. ferrooxidans. Based upon their sequences, the 313-residue GnnA protein was proposed to catalyze the NAD(+)-dependent oxidation of the glucosamine 3-OH of UDP-GlcNAc, and the 369-residue GnnB protein was proposed to catalyze the subsequent transamination to form UDP 2-acetamido-3-amino-2,3-dideoxy-alpha-d-glucopyranose (UDP-GlcNAc3N). Both gnnA and gnnB were cloned and expressed in E. coli using pET23c+. In the presence of l-glutamate and NAD(+), both proteins were required for the conversion of [alpha-(32)P]UDP-GlcNAc to a novel, less negatively charged sugar nucleotide shown to be [alpha-(32)P]UDP-GlcNAc3N. The latter contained a free amine, as judged by modification with acetic anhydride. Using recombinant GnnA and GnnB, approximately 0.4 mg of the presumptive UDP-GlcNAc3N was synthesized. The product was purified and subjected to NMR analysis to confirm the replacement of the GlcNAc 3-OH group with an equatorial NH(2). As shown in the accompanying papers, UDP-GlcNAc3N is selectively acylated by LpxAs of A. ferrooxidans, L. interrogans, and M. loti. UDP-GlcNAc3N may be useful as a substrate analog for diverse enzymes that utilize UDP-GlcNAc.  (+info)

Apparent redundancy of electron transfer pathways via bc(1) complexes and terminal oxidases in the extremophilic chemolithoautotrophic Acidithiobacillus ferrooxidans. (7/101)

Acidithiobacillus ferrooxidans is an acidophilic chemolithoautotrophic bacterium that can grow in the presence of either the weak reductant Fe(2+), or reducing sulfur compounds that provide more energy for growth than Fe(2+). We have previously shown that the uphill electron transfer pathway between Fe(2+) and NAD(+) involved a bc(1) complex that functions only in the reverse direction [J. Bacteriol. 182, (2000) 3602]. In the present work, we demonstrate both the existence of a bc(1) complex functioning in the forward direction, expressed when the cells are grown on sulfur, and the presence of two terminal oxidases, a bd and a ba(3) type oxidase expressed more in sulfur than in iron-grown cells, besides the cytochrome aa(3) that was found to be expressed only in iron-grown cells. Sulfur-grown cells exhibit a branching point for electron flow at the level of the quinol pool leading on the one hand to a bd type oxidase, and on the other hand to a bc(1)-->ba(3) pathway. We have also demonstrated the presence in the genome of transcriptionally active genes potentially encoding the subunits of a bo(3) type oxidase. A scheme for the electron transfer chains has been established that shows the existence of multiple respiratory routes to a single electron acceptor O(2). Possible reasons for these apparently redundant pathways are discussed.  (+info)

Regulation of the expression of the Acidithiobacillus ferrooxidans rus operon encoding two cytochromes c, a cytochrome oxidase and rusticyanin. (8/101)

The regulation of the expression of the rus operon, proposed to encode an electron transfer chain from the outer to the inner membrane in the obligate acidophilic chemolithoautroph Acidithiobacillus ferrooxidans, has been studied at the RNA and protein levels. As observed by Northern hybridization, real-time PCR and reverse transcription analyses, this operon was more highly expressed in ferrous iron- than in sulfur-grown cells. Furthermore, it was shown by immunodetection that components of this respiratory chain are synthesized in ferrous iron- rather than in sulfur-growth conditions. Nonetheless, weak transcription and translation products of the rus operon were detected in sulfur-grown cells at the early exponential phase. The results strongly support the notion that rus-operon expression is induced by ferrous iron, in agreement with the involvement of the rus-operon-encoded products in the oxidation of ferrous iron, and that ferrous iron is used in preference to sulfur.  (+info)

Acidithiobacilli are acidophilic microorganisms that play important roles in many natural processes such as acidification of the environment, influencing metal mobility, and impacting on global sulfur and iron cycles. Due to their distinct metabolic properties they can be applied in the industrial extraction of valuable metals. Acidithiobacilli thrive in an environment which is extremely acidic and usually low in organic carbon but highly polluted with metals. In the quest to gain insight into how these microorganisms can thrive in their extreme environment, relevant facets of metabolism, metal resistance, and pH homeostasis were exploredwith the focus on two model organisms,. Acidithiobacillus caldus and Acidithiobacillus ferrooxidans. Understanding these fundamental aspects of an acidophilic lifestyle will help to eventually control detrimental effects on the environment due to acidification and metal pollution as well as improving metal extraction utilizing acidophilic ...
TY - JOUR. T1 - Purification and biochemical characterization of the F1-ATPase from Acidithiobacillus ferrooxidans NASF-1 and analysis of the atp operon. AU - Wakai, Satoshi. AU - Ohmori, Asami. AU - Kanao, Tadayoshi. AU - Sugio, Tsuyoshi. AU - Kamimura, Kazuo. PY - 2005/10/23. Y1 - 2005/10/23. N2 - ATPase was purified 51-fold from a chemoautotrophic, obligately acidophilic iron-oxidizing bacterium, Acidithiobacillus ferrooxidans NASF-1. The purified ATPase showed the typical subunit pattern of the F1-ATPase on a polyacrylamide gel containing sodium dodecyl sulfate, with 5 subunits of apparent molecular masses of 55, 50, 33, 20, and 18 kDa. The enzyme hydrolyzed ATP, GTP, and ITP, but neither UTP nor ADP. The Km, value for ATP was 1.8 mM. ATPase activity was optimum at pH 8.5 at 45°C, and was activated by sulfite. Azide strongly inhibited the enzyme activity, whereas the enzyme was relatively resistant to vanadate, nitrate, and N,N′- dicyclohexylcarbodiimide. The genes encoding the subunits ...
The ex-situ bioremoval of U(VI) from contaminated water using Acidithiobacillus ferrooxidans strain 8455 and 13538 was studied under a range of pH and uranium concentrations. The effect of pH on the growth of bacteria was evaluated across the range 1.5 - 4.5 pH units. The respiration rate of At. ferrooxidans at different U(VI) concentrations was quantified as a measure of the rate of metabolic activity over time using an oxygen electrode. The biosorption process was quantified using a uranyl nitrate solution, U-spiked growth media and U-contaminated mine water. The results showed that both strains of At. ferrooxidans are able to remove U(VI) from solution at pH 2.5 - 4.5, exhibiting a buffering capacity at pH 3.5. The respiration rate of the micro-organism was affected at U(VI) concentration of 30 mg L-1. The kinetics of the sorption fitted a pseudo-first order equation, and depended on the concentration of U(VI). The KD obtained from the biosorption experiments indicated that strain 8455 is more
Domain architecture and assignment details (superfamily, family, region, evalue) for gi|198282829|ref|YP_002219150.1| from Acidithiobacillus ferrooxidans ATCC 53993. Plus protein sequence and external database links.
Interaction of acidithiobacillus ferrooxidans, rhizobium phaseoli and rhodotorula sp. in bioleaching process based on lotka-volterra model . Dongwei Li ;Electronic Journal of Biotechnology 2016, 22. Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
The acidithiobacilli are sulfur-oxidizing acidophilic bacteria that thrive in both natural and anthropogenic low pH environments. They contribute to processes that lead to the generation of acid rock drainage in several different geoclimatic contexts, and their properties have long been harnessed for the biotechnological processing of minerals. Presently, the genus is composed of seven validated species, described between 1922 and 2015: Acidithiobacillus thiooxidans, A. ferrooxidans, A. albertensis, A. caldus, A. ferrivorans, A. ferridurans and A. ferriphilus. However, a large number of Acidithiobacillus strains and sequence clones have been obtained from a variety of ecological niches over the years, and many isolates are thought to vary in phenotypic properties and cognate genetic traits. Moreover, many isolates remain unclassified and several conflicting specific assignments muddle the picture from an evolutionary standpoint. Here we revise the phylogenetic relationships within this species complex
Acidithiobacillus ferrooxidans has emerged as an economically significant bacterium in the field of biohydrometallurgy, in the leaching of sulfide ores since its discovery in 1950 by Colmer, Temple and Hinkle. The discovery of A. ferrooxidans led to the development of "biohydrometallurgy", which deals with all aspects of microbial mediated extraction of metals from minerals or solid wastes and acid mine drainage.[5] A. ferrooxidans has been proven as a potent leaching organism, for dissolution of metals from low-grade sulfide ores. Recently, the attention has been focused upon the treatment of mineral concentrates, as well as complex sulfide ores using batch or continuous-flow reactors. Acidithiobacillus ferrooxidans is commonly found in acid mine drainage and mine tailings. The oxidation of ferrous iron and reduced sulfur oxyanions, metal sulfides and elementary sulfur results in the production of ferric sulfate in sulfuric acid, this in turn causes the solubilization of metals and other ...
Arsenopyrite (FeAsS) and realgar (As4S4) are two common arsenic minerals that often cause serious environmental issues. Centralised treatment of arsenic-containing tailings can reduce land occupation and save management costs. The current work examined the remediation schemes of tailings from Hunan Province, China, where by different tailings containing arsenopyrite and realgar were blended with exogenous slag zero valence iron (ZVI). Introducing Fe-oxidising bacteria (Acidithiobacillus ferrooxidans) recreates a biologically oxidative environment. All bioleaching experiments were done over three stages, each for 7 days and the solid phase of all tests was characterised by scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, X-ray photoelectron spectroscopy and selective extraction analyses. The results showed that the mixture group reduced arsenic release by 72.9-74.7% compared with the control group. The addition of 0.2 g ZVI clearly decreased arsenic ...
A combination of EXAFS, transmission electron microscopy and energy-dispersive X-ray was used to conduct a molecular and atomic analysis of the uranium complexes formed by Acidithiobacillus ferrooxidans. The results demonstrate that this bacterium accumulates uranium as phosphate compounds. We suggest that at toxic levels when the uranium enters the bacterial cells, A. ferrooxidans can detoxify and efflux this metal by a process in which its polyphosphate bodies are involved. ...
Sulfur is an essential element for life and the metabolism of organic sulfur compounds plays an important role in the global sulfur cycle. Sulfur occurs in various oxidation states ranging from +6 in sulfate to -2 in sulfide (H2S). Sulfate reduction can occur in both an energy consuming assimilatory pathway and an energy producing dissimilatory pathway. The assimilatory pathway, which is found in a wide range of organisms, produces reduced sulfur compounds for the biosynthesis of S-containing amino acids and does not lead to direct excretion of sulfide. In the dissimilatory pathway, which is restricted to obligatory anaerobic bacterial and archaeal lineages, sulfate (or sulfur) is the terminal electron acceptor of the respiratory chain producing large quantities of inorganic sulfide. Both pathways start from the activation of sulfate by reaction with ATP to form adenylyl sulfate (APS). In the assimilatory pathway [MD:M00176] APS is converted to 3-phosphoadenylyl sulfate (PAPS) and then reduced ...
TABLE-US-00002 Program: CLUSTALW, Default parameters: Protein Gap Open Penalty 10.0 Protein Gap Extension Penalty 0.2 Protein weight matrix: Gonnet series GI No. of the reference S_ID DB SEQ ID NO Organism sequences Aa Position s1 seq_ID 2 Zymomonas mobilis AAV90172.1 F 486 s20 seq_ID 3 Streptomyces coelicolor CAB39697.1 F 449 s911 seq_ID 4 Acetobacter pasteurianus BAH99456.1 F 481 s2 seq_ID 5 Bradyrhizobium sp. ABQ33590.1 F 447 s940 seq_ID 6 Zymomonas mobilis EER62728.1 F 438 s949 seq_ID 7 Acidithiobacillus caldus EET25937.1 Y 432 s167 seq_ID 8 Acidithiobacillus ferrooxidans ACH84004.1 Y 429 s41 seq_ID 9 Acidobacterium capsulatum ACO34244.1 F 458 s36 seq_ID 10 Acidothermus cellulolyticus ABK53469.1 F 426 s83 seq_ID 11 Adiantum capillus-veneris BAF93209.1 Y 436 s143 seq_ID 12 Ajellomyces capsulatus EDN09769.1 F 496 s995 seq_ID 13 Ajellomyces capsulatus EER40510.1 -- 432 s163 seq_ID 14 Ajellomyces capsulatus EEH02950.1 F 429 s13 seq_ID 15 Alicyclobacillus acidocaldarius EED08231.1 Y 420 s14 ...
Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.
This study investigated the improvement in dewaterability of activated sludge (ACS) and anaerobically digested sludge (ADS) through bioacidification approach using iron-oxidizing bacterium, Acidithiobacillus ferrooxidans. ACS and ADS were treated with A. ferrooxidans culture with addition of different concentrations of energy substrate, in terms of Fe(2+):sludge solids ratio (0:1, 0.01:1, 0.05:1 and 0.1:1), and the dewaterability was assessed by determining the capillary suction time (CST), time to filter (TTF) and specific resistance to filtration (SRF) of the sludge. The results revealed that the levels of Fe(2+) significantly influenced the sludge acidification (pH⩽3). The CST, TTF and SRF values rapidly decreased in treated sludge, indicating that dewaterability of the sludge was significantly (p|0.05) improved than untreated sludge. This investigation clearly demonstrates that A. ferrooxidans culture, as biogenic flocculant, can be potentially used for improving the sludge flocculation,
Various methods of nucleic acid (NA) extraction were investigated with the aim of developing a quantitative method of NA extraction from five representative strains of biomining microorganisms. The process of removing cells from mineral surfaces, lysing microorganisms, precipitating NA and purifying RNA were analysed. The success of each method was examined spectrophotometrically, by agarose gel electrophoresis and PCR or quantitative real time PCR (qPCR). The most important step was shown to be cellular lysis, which principally impacted on thequantity of NA extracted from each strain. The quantity and quality of extracted NA was highlydependent on the method of NA precipitation. This study resulted in the development of a NA extraction method that reliably and reproducibly extracted NA from five strains of biomining microorganisms.. ...
Zammit, C. and Mutch, L. and Watling, H. and Watkin, E. 2008. Evaluation of quantitative real-time polymerase chain reaction for enumeration of biomining microorganisms in culture. Hydrometallurgy. 94 (1-4): pp. 185-189 ...
A series of bacterial and chemical leaching experiments were conducted to clarify contradictory reports in the literature regarding the role of bacteria in the bioleaching of chalcopyrite. Tests containing a high bacterial concentration showed inhibited leaching, even lower than non-inoculated controls. However, when bacterial cells were washed before inoculation, it was apparent that it was not the bacterial cells but rather the chemical species introduced with them that influenced the leaching rate. In addition, the results of comparative tests with 0.1 M ferrous sulphate or ferric sulphate showed that copper was leached from the ore 2.7 times faster in leach solutions containing ferrous ion, suggesting that ferric ions inhibit chalcopyrite dissolution. The results indicated that the chalcopyrite dissolution rate is strongly dependent on the reduction potential (Eh) in solution, and that this parameter is far more influential than the number or activity of bacterial cells. These results imply ...
Oxidative dissolution of chalcopyrite at ambient temperatures is generally slow and subject to passivation, posing a major challenge for developing bioleaching applications for this recalcitrant mineral. Chloride is known to enhance the chemical leaching of chalcopyrite, but much of this effect has been demonstrated at elevated temperatures. This study was undertaken to test whether 100-200 mM Na-chloride enhances the chemical and bacterial leaching of chalcopyrite in shake flasks and stirred tank bioreactor conditions at mesophilic temperatures. Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and abiotic controls were employed for the leaching experiments. Addition of Na-chloride to the bioleaching suspension inhibited the formation of secondary phases from chalcopyrite and decreased the Fe(III) precipitation. Neither elemental S nor secondary Cu-sulfides were detected in solid residues by X-ray diffraction. Chalcopyrite leaching was enhanced when the solution contained bacteria, ...
Several archaeal species from the order Sulfolobales are interesting from the biotechnological point of view due to their biomining capacities. Within this group, the genus Acidianus contains four biomining species (from ten known Acidianus species), but none of these have their genome sequenced. To get insights into the genetic potential and metabolic pathways involved in the biomining activity of this group, we sequenced the genome of Acidianus copahuensis ALE1 strain, a novel thermoacidophilic crenarchaeon (optimum growth: 75 °C, pH 3) isolated from the volcanic geothermal area of Copahue at Neuquén province in Argentina. Previous experimental characterization of A. copahuensis revealed a high biomining potential, exhibited as high oxidation activity of sulfur and sulfur compounds, ferrous iron and sulfide minerals (e.g.: pyrite). This strain is also autotrophic and tolerant to heavy metals, thus, it can grow under adverse conditions for most forms of life with a low nutrient demand, conditions
references say that the former increases the rate of pyrite oxidation causing lakes to be come acidic. While other resources say its the latter. Maybe they are the same? but if they are the same, how come the former also increases the rate of metal precipitation and reduces the acidity of acidic lakes by reduction of sulfate when it causes the acidity in the first place ...
FIG. 1. Sequence alignment of porins from α-, β-, and γ-proteobacteria. The classification of the source organisms into γ-, β-, and α-subdivisions is shown at the beginning. Transmembrane β-strands are indicated by highlighting, in red, the presence of hydrophobic amino acid residues at alternate positions. The PEFGGD motif of loop 3 in the Enterobacteriaceae and corresponding sequences are colored in blue. The alignment of E. coli OmpF (ECOOMPF), E. coli OmpC (ECOOMPC), E. coli PhoE (ECOPHOE), Haemophilus influenzae Rd P2 porin (HINP2), Neisseria meningitidis PorB (NMEPORB), Bordetella pertussis porin (BPEPOR), Comamonas acidovorans Omp32 (CACPOR), Rhodopseudomonas blastica porin (RBLPOR), and Rhodobacter capsulatus porin (RCAPOR) is basically that of Jeanteur et al. (303, 304), with minor adjustments. The rest of the sequences (Acidithiobacillus ferrooxidans porin [AFEPOR], Pasteurella multocida OmpH [PMUOMPH], Serratia marcescens OmpF [SMAOMPF], Vibrio cholerae OmpU [VCHOMPU], ...
The extraction of metals from ores causes various environmental pollutions. Since Iran is located on the so-called copper belt and holds a significant share of the worlds copper mines and resources, reduction of pollution from these mines can have an important effect on the overall reduction of pollution. Copper processing methods include pyrometallurgy and hydrometallurgy. Pyrometallurgy is mainly used in high grade mines whereas hydrometallurgy process is used in lower grade mines. In low grade copper sulfide mines, hydrometallurgy processes are used which use a lot of energy to covert mineral deposits into oxide forms which are then leached using sulfuric acid, or are extracted using bioleaching process. In acidic leaching, a lot of environmental pollution is created. Bioleaching process is an environmentally-friendly method which is mainly used in mines where the common physicochemical methods are not profitable. In this study, we have tried to increase the efficiency of bioleaching process by
Synonyms for Acidophils in Free Thesaurus. Antonyms for Acidophils. 1 synonym for acidophil: acidophile. What are synonyms for Acidophils?
Looking for acidophil neoplasms? Find out information about acidophil neoplasms. Any substance, tissue, or organism having an affinity for acid stains. An organism having a preference for an acid environment. An alpha cell of the... Explanation of acidophil neoplasms
Pyrites \Py*rites\, n. [L., fr. Gr. ?, fr. ? fire. See {Pyre}.] (Min.) A name given to a number of metallic minerals, sulphides of iron, copper, cobalt, nickel, and tin, of a white or yellowish color. [1913 Webster] Note: The term was originally applied to the mineral pyrite, or iron pyrites, in allusion to its giving sparks when struck with steel. [1913 Webster] {Arsenical pyrites}, arsenopyrite. {Auriferous pyrites}. See under {Auriferous
The founder, Afe Babalola University, Ado Ekiti, Chief Afe Babalola, has advised the Federal Government against waiting till all towns and villages in the country are free from COVID-19 before reopening schools. Babalola listed the conditions under which
Our January Meeting: The Lowdown on Sulphur with David Bellamy Featured Image: Sulphur Crystal: Courtesy David Bellamy The January meeting will take place on January 3rd at […]
These two fields allow to specify a different default assignee for ticket opened against this package in bugzilla. Note: The EPEL field is always displayed for packages in the rpms namespace regardless of whether it is used in bugzilla or not. ...
Snottites. These stalactite-like formations are made from biofilms of extremophilic bacteria. They have a mucus-like consistency, hence the common name. The bacteria metabolise sulphur compounds for energy, producing very acidic byproducts. - Stock Image C021/0740
Some sulfate-reducing bacteria produce hydrogen sulfide, which can cause sulfide stress cracking. Acidithiobacillus bacteria produce sulfuric acid; Acidothiobacillus thiooxidans frequently damages sewer pipes. Ferrobacillus ferrooxidans directly oxidizes iron to iron oxides and iron hydroxides; the rusticles forming on the RMS Titanic wreck are caused by bacterial activity. Other bacteria produce various acids, both organic and mineral, or ammonia. In presence of oxygen, aerobic bacteria like Acidithiobacillus thiooxidans, Thiobacillus thioparus, and Thiobacillus concretivorus, all three widely present in the environment, are the common corrosion-causing factors resulting in biogenic sulfide corrosion. Without presence of oxygen, anaerobic bacteria, especially Desulfovibrio and Desulfotomaculum, are common. Desulfovibrio salixigens requires at least 2.5% concentration of sodium chloride, but D. vulgaris and D. desulfuricans can grow in both fresh and salt water. D. africanus is another common ...
Phototrophic Fe(II)-oxidizing bacteria couple the oxidation of ferrous iron [Fe(II)] to reductive CO2 fixation by using light energy. Rhodopseudomonas palustris TIE-1 has a three-gene operon, designated the pio operon (for phototrophic iron oxidation), that is necessary for phototrophic Fe(II) oxidation. The first gene in the operon, pioA, encodes a c-type cytochrome that is upregulated under Fe(II)-grown conditions. PioA contains a signal sequence and shares homology with MtrA, a decaheme c-type cytochrome from Shewanella oneidensis MR-1 (TC# 5.B.5.1.1). The second gene, pioB, encodes an outer membrane β-barrel protein, a homologue of MtrB from S. oneidensis MR-1. The third gene, pioC, encodes a high potential iron sulfur protein (HiPIP) with a twin-arginine translocation (Tat) signal sequence and is similar to the Fe(II) oxidoreductase (Iro) from Acidithiobacillus ferrooxidans. Like PioA, PioB and PioC appear to be secreted proteins. Deletion of the pio operon results in loss of Fe(II) ...
Transformation of plasmids of Acidithiobacillus ferrooxidans encoding ampicillin resistance to. National Institute for Biotechnology and Genetic Engineering, P.Impact of three ampicillin dosage regimens on selection of ampicillin resistance in Enterobacteriaceae and excretion of blaTEM genes in swine feces.. Antibiotic resistance is a type of drug resistance where a. The most commonly used antibiotics in genetic engineering are generally "older. ampicillin kanamycin.Ampicillin generic name. Best Online Pharmacies and conditions for buying drugs online. Find us on FaceBook!.Jeux Alchemy gratuit: le jeu en ligne Alchemy est en flash et est jouable sans aucun telechargement ni inscription ...
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The species of the genus 'Thiobacillus' fall into the alpha-, beta- and gamma-subclasses of the Proteobacteria, the type species Thiobacillus thioparus being located in the beta-subclass. 'Thiobacillus' species exhibit almost as much diversity in DNA composition and physiology as is found collectively in all other proteobacterial groups. On the basis of physiological characters and 16S rRNA gene sequence comparisons, eight of the existing Thiobacillus species are proposed for reassignment to three newly designated genera within the gamma-subclass of the Proteobacteria, namely Acidithiobacillus, Halothiobacillus and Thermithiobacillus.
Spent refinery processing catalyst is listed as a hazardous waste; the toxicity characteristic leaching procedure (TCLP) extracts of the catalyst are found to contain heavy metals at concentrations exceeding the regulated levels. In the present investigation, Aspergillus niger was adapted to single metal ions Ni, Mo or Al (at 100-2000 mg/L in steps of 100 mg/L) and then to a mixture of Ni, Mo and Al (at a mass ratio of 1:2:6, as approximately present in the spent catalyst). Adaptation experiments with single metals showed that the fungus could tolerate up to 1000 mg/L Ni, 1200 mg/L Mo and 2000 mg/L Al. In the presence of a mixture of these metals, the fungus was able to tolerate up to 100 mg/L Ni, 200 mg/L Mo and 600 mg/L Al. One-step bioleaching experiments with 1 wt% spent catalyst (of particle size <37 μm) were carried out using un-adapted and various adapted fungal strains. In contrast to the adapted strains, the un-adapted strain showed no growth in the presence of the catalyst. ...
Extremophile fishes have emerged as veritable models for investigations in integrative biology. They not only provide insights into biochemical, physiological, and developmental processes that govern...
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La sphalérite et larsénopyrite dun système hydrothermal nourricier associé au gisement de sulfures massifs de Brunswick No. 12 (camp minier de Bathurst, Nouveau-Brunswick), maintenant transposé et recristallisé, sont typiquement associées à un assemblage de pyrite, pyrrhotite, chalcopyrite, quartz, et une chlorite ferrifère. Les résultats danalyses texturales et chimiques de la sphalérite et larsénopyrite incluses dans la pyrite métamorphique en coexistence avec la pyrrhotite indiquent une variation importante en pression entre les conditions épigénétiques, précoces, et celles de la recristallisation au début de lévénement D1/M1. La teneur de la sphalérite en XFeS, variant entre 11.4 et 15.2% (base molaire), concorde avec une pression de 770 à 440 MPa, qui représenterait la pression maximale et la pression à un stade tardif de lévénement D1, respectivement. La préservation locale dune intercroissance squelettique darsénopyrite et de ...
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The most distinct difference between gold and pyrite is in their hardness. Gold is one of the most malleable and ductile metals on earth. The mineral can take any shape when flattened and is easily scratched with a pocket knife.. Pyrite is noticeably harder with a hardness of 6 on Mohs scale while gold is at a 3. Pyrite is not easily scratched and will shatter like glass if struck by a hammer. In fact, if you take a piece of copper and scratch it with a piece of pyrite, it will a scratch on the copper. If you repeat the test with gold instead of pyrite, the gold will be scratched.. Spotting the differences between gold and pyrite takes practice - but a little know-how will take you a long way. Next time youre digging for treasure and youve found a golden nugget, youll be ready with the on-site tools needed to tell the difference.. ...
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Pyrite triplet Wagnerite is a powerful little stone. It assists in time of fatigue and burn out.. The energy of Pyrite triplet Wagnerite supports the base, sacral and solar plexus chakras, especially if these chakra centres have experienced stressful energy .. ...
Well, yes...between sulfate, SO_4^(2-), and sulfite, SO_3^(2-), ions......? .......These differ by oxidation state. SO_4^(2-) exhibits a S(+VI) oxidation state. SO_3^(2-) exhibits a S(+IV) oxidation state. Of course, you can see the difference in the number of oxygen atoms of each species. See here for a link to similar answers. If you dont know how to assign oxidation states, see here and here. Good luck!
... _Mineralogy of Sulfides ElementsMineralogy of Sulfides ... a general formula A m T n X p and in which the common elements are A = Ag ... along the c-axis direction in pyrite (FeS 2 ...Iron disul
If_ty: \,Gamma, \,turnstile, b \,Longrightarrow, \,Gamma, \,turnstile, c1 \,Longrightarrow, \,Gamma, \,turnstile, c2 \,Longrightarrow, \,Gamma, \,turnstile, IF b THEN c1 ELSE c2 ...
Our MDV™ technology uses smooth bends and directional vanes to form multiple air paths through the manifold. These new paths are smoother and shorter, producing much smaller dead air zones for increased overall airflow to the engine ...
PhD title: Search for new antibiotics in extremophilic worms and studying their adaptation and functions in extreme habitats *Deadline for application: June 1, 2016* *A. ...
Brian A. Kelch, Kyle P. Eagen, F. Pinar Erciyas, Elizabeth L. Humphris, Adam R. Thomason, Shinji Mitsuiki, David A. Agard. Structural and Mechanistic Exploration of Acid Resistance: Kinetic Stability Facilitates Evolution of Extremophilic Behavior. Journal of Molecular Biology (2007) 368, 870-883. Cover Article Full Text ...
afe for students and teachers, this kit is ideal for use in the lab or in the field. Semi-hard, slotted, plastic case can be sanitized and has... (53874)
anja serve (diplom) - phd student at the [http://www.mpi-magdeburg.mpg.de/ Max Planck Institute for Dynamics of Complex Technical Systems] ([http://www.ovgu.de/ivt/bpt/index.php?idcatside=110&lang=2&sid=5ade3bba2fffd83afe84d2de1cca6749 Chair of Bioprocess Engineering]),br ...
Acidithiobacillus bacteria produce sulfuric acid; Acidothiobacillus thiooxidans frequently damages sewer pipes. Ferrobacillus ... In presence of oxygen, aerobic bacteria like Acidithiobacillus thiooxidans, Thiobacillus thioparus, and Thiobacillus ...
Power, Ian M.; Dipple, Gregory M.; Southam, Gordon (2010). "Bioleaching of Ultramafic Tailings by Acidithiobacillus spp. For ...
Taha, T.M.; Kanao, T.; Takeuchi, F.; Sugio, T. (2008). "Reconstitution of iron oxidase from sulfur-grown Acidithiobacillus ... Yarzabal, A.; Appia-Ayme, C.; Ratouchniak, J.; Bonnefoy, V. (2004). "Regulation of the expression of the Acidithiobacillus ... "The high-molecular-weight cytochrome c Cyc2 of Acidithiobacillus ferrooxidans is an outer membrane protein". J. Bacteriol. 184 ... "Extending the models for iron and sulfur oxidation in the extreme acidophile Acidithiobacillus ferrooxidans". BMC Genomics. 10 ...
fungi and sulfur removal from coal with Acidithiobacillus sp.. The extraction can occur at the mine site, from waste water ... Genera such as Acidithiobacillus and Leptospirillum bacteria, and Thermoplasmatales archaea, are present in syntrophic ... Other bacteria also implicated in AMD include Leptospirillum ferrooxidans, Acidithiobacillus thiooxidans and Sulfobacillus ... particularly Acidithiobacillus ferrooxidans (synonym Thiobacillus ferrooxidans). These bacteria can accelerate pyritic ...
In particular, Acidithiobacillus ferrooxidans is a key contributor to pyrite oxidation. Metal mines may generate highly acidic ... Colonization of pyrite by Acidithiobacillus ferrooxidans under pH-neutral conditions". Geobiology. 1 (1): 81-90. doi:10.1046/j. ...
Acidithiobacillus ferrooxidans is able to oxidize the Cu+ in chalcocite (Cu2S) to Cu2+, thus removing some of the copper in the ... Using Bacteria such as Acidithiobacillus ferrooxidans to leach copper from mine tailings has improved recovery rates and ... Using Bacteria such as Acidithiobacillus ferrooxidans to leach copper from mine tailings has improved recovery rates and ... Using Bacteria such as Acidithiobacillus ferrooxidans to leach copper from mine tailings has improved recovery rates and ...
Acidithiobacillus, Thermithiobacillus spp. are unable to oxidise ferrous iron or iron-containing minerals. The genome sequence ... "Reclassification of some species of Thiobacillus to the newly designated genera Acidithiobacillus gen. nov., Halothiobacillus ...
"Reclassification of some species of Thiobacillus to the newly designated genera Acidithiobacillus gen. nov., Halothiobacillus ...
... quinone oxidoreductase from Acidithiobacillus ferrooxidans: insights into sulfidotrophic respiration and detoxification". J. ...
ISBN 978-0-12-026147-5. Meruane G, Vargas T (2003). "Bacterial oxidation of ferrous iron by Acidithiobacillus ferrooxidans in ...
The bacterial culture is a mixed culture of Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Leptospirillum ...
... and molecular characterization of the NAD+-dependent isocitrate dehydrogenase from the chemolithotroph Acidithiobacillus ...
... and Acidithiobacillus ferrooxidans. In 2001, Prof. Chakrabarty founded a company, CDG Therapeutics, (incorporated in Delaware) ...
Studies in Sulfobacillus thermosulfidooxidans and Acidithiobacillus caldus". Microorganisms. 3: 707-724. Han, Yuchen; Perner, ...
Due to a single genus, Acidithiobacillus, the Gammaproteobacteria class is paraphyletic to Betaproteobacteria (reviewed in ...
Power, I.M., Dipple, G.M., and Southam, G. (2010) Bioleaching of ultramafic tailings by Acidithiobacillus spp. for CO2 ... Bioleaching of non-sulfidic ores by layering of waste sulfides and elemental sulfur, colonized by Acidithiobacillus spp., has ... Bioleaching can involve numerous ferrous iron and sulfur oxidizing bacteria, including Acidithiobacillus ferrooxidans (formerly ... known as Thiobacillus ferrooxidans) and Acidithiobacillus thiooxidans (formerly known as Thiobacillus thiooxidans). As a ...
The Acidithiobacillales are an order of bacteria within the class Acidithiobacillia and comprises the genera Acidithiobacillus ... Reclassification of some species of Thiobacillus to the newly designated genera Acidithiobacillus gen. nov., Halothiobacillus ...
"The sulfane sulfur of persulfides is the actual substrate of the sulfur-oxidizing enzymes from Acidithiobacillus and ...
The first are acidophiles, such as the bacteria Acidithiobacillus ferrooxidans and Leptospirillum ferrooxidans, as well as the ...
The genus Acidithiobacillus, part of the Gammaproteobacteria until it was transferred to class Acidithiobacillia in 2013, was ... which includes economically important organisms used in the mining industry such as Acidithiobacillus spp. The ... Acidithiobacillia: Acidithiobacillus thiooxidans, Thermithiobacillus tepidarius Hydrogenophilalia: Hydrogenophilus ...
This process is hastened by the action of Acidithiobacillus bacteria which oxidize the pyrite to produce ferrous iron and ...
The chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans, that lives in ocean thermal vents, has been shown to ... "Various Growth Aspects of Acidithiobacillus Ferrooxidans" http://journal.frontiersin.org/article/10.3389/fmicb.2015.00994/full ...
Acidithiobacillus ferrooxidans Fe2+ (ferrous iron) → Fe3+ (ferric iron) + e−[20]. O. 2 (oxygen) + 4H+ + 4e−→ 2H. 2O [20]. ... "Bacterial oxidation of ferrous iron by Acidithiobacillus ferrooxidans in the pH range 2.5-7.0" (PDF). Hydrometallurgy. 71 (1 ...
"Reclassification of some species of Thiobacillus to the newly designated generus Acidithiobacillus gen. nov., Halothiobacillus ...
... are used by various lithotrophic bacteria and are all oxidized by Acidithiobacillus. Sulfur oxidizers utilize enzymes such as ...
Acidithiobacillus caldus and Acidithiobacillus ferrooxidans. Understanding these fundamental aspects of an acidophilic ... Sulfur metabolism in the extreme acidophile acidithiobacillus caldus. Mangold, Stefanie Umeå University, Faculty of Science and ... oxidation was initiated in the chemolithoautotrophic extremophile Acidithiobacillus caldus. A. caldus is able to metabolize ...
... from contaminated water using Acidithiobacillus ferrooxidans strain 8455 and 13538 was studied under a range of pH and uranium ... from contaminated water using Acidithiobacillus ferrooxidans strain 8455 and 13538 was studied under a range of pH and uranium ... Effect of Solution pH and Oxygen on the Activity of Acidithiobacillus ferrooxidans. Acidithiobacillus ferroxidans has great ... Effect of pH on the Growth of Acidithiobacillus ferrooxidans. The effect of variation of initial solution pH on the growth of ...
... from Acidithiobacillus ferrooxidans ATCC 53993. Plus protein sequence and external database links. ... Domain assignment for gi,198282829,ref,YP_002219150.1, from Acidithiobacillus ferrooxidans ATCC 53993. Domain architecture ...
keywords = "Acidithiobacillus ferrooxidans, Acidophile, atp operon, FF -ATPase",. author = "Satoshi Wakai and Asami Ohmori and ... Purification and biochemical characterization of the F1-ATPase from Acidithiobacillus ferrooxidans NASF-1 and analysis of the ... Purification and biochemical characterization of the F1-ATPase from Acidithiobacillus ferrooxidans NASF-1 and analysis of the ... Purification and biochemical characterization of the F1-ATPase from Acidithiobacillus ferrooxidans NASF-1 and analysis of the ...
Acidithiobacillus albertensis Acidithiobacillus caldus Acidithiobacillus ferridurans Acidithiobacillus ferriphilus ... Genus Acidithiobacillus[edit]. Acidithiobacillus are acidophilic obligate autotrophs (Acidithiobacillus caldus can also grow ... Acidithiobacillus is a genus of the Acidithiobacillia in the "Proteobacteria". Like all "Proteobacteria", Acidithiobacillus spp ... Acidithiobacillus thiooxidans (basonym Thiobacillus thiooxidans, Thiobacillus concretivorus[1]) oxidises sulfur and produces ...
All three Acidithiobacillus species were differentiated by phylogenetic analysis of the ITS sequences. The size and sequence ... Acidithiobacillus thiooxidans, formerly known as Thiobacillus thiooxidans until its reclassification into the newly designated ... Acidithiobacillus thiooxidans strains have been differentiated from other related Acidithiobacilli, including A. ferrooxidans ... Most of the information about Acidithiobacillus comes from experimental and genome-based analyses of two other related species ...
Acidithiobacillus ferrooxidans strain YPS9-4 16S-23S ribosomal RNA intergenic sp... Acidithiobacillus ferrooxidans strain YPS9- ... Acidithiobacillus ferrooxidans strain YPS9-4 16S-23S ribosomal RNA intergenic spacer, partial sequence. GenBank: KX756696.1 ...
Homologous recombination - Acidithiobacillus caldus SM-1 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
Sulfur metabolism - Acidithiobacillus caldus SM-1 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
Acidithiobacillus sp. GGI-221. mine drainage metagenome. Acidithiobacillus ferrivorans. Acidithiobacillus ferrivorans SS3. ... Acidithiobacillus sp. GGI-221. mine drainage metagenome. 205. UniRef100_B7JBX0. Cluster: LexA repressor. 4. ... Acidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993)). ,p>This subsection of the ,a ... sp,B5EJ91,LEXA_ACIF5 LexA repressor OS=Acidithiobacillus ferrooxidans (strain ATCC 53993) OX=380394 GN=lexA PE=3 SV=1 ...
Acidithiobacillus thiooxidans (type strain ATCC 19377) Description and significance. Acidithiobacillus thiooxidans was once ... Acidithiobacillus thiooxidans are most commonly located in soil, sewer pipes and cave biofilms also known as "snottites". ... Difference between revisions of "Acidithiobacillus thiooxidans". From MicrobeWiki, the student-edited microbiology resource ... Figure 1. Electron microscope image of Acidithiobacillus thiooxidans. From Khan et al., 2012. ...
Acidithiobacillus ferrooxidans (Thiobacillus ferrooxidans). Acidithiobacillus ferrivorans SS3. Acidithiobacillus ferrivorans. ... Acidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993)). Acidithiobacillus ferrooxidans ( ... Acidithiobacillus ferrooxidans (Thiobacillus ferrooxidans). ,p>This subsection of the Names and taxonomy section shows the ... sp,Q56275,GLMS_ACIFR Glutamine--fructose-6-phosphate aminotransferase [isomerizing] OS=Acidithiobacillus ferrooxidans GN=glmS ...
The chemolithoautotrophic Gram-negative bacterium Acidithiobacillus ferrooxidans is versatile and can grow on a number of ...
Acidithiobacillus ferrooxidans ATCC 23270. Mutation(s): 0 Gene Names: AFE_1792. EC: 1.8.5.4. ... Find proteins for B7JBP8 (Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / CIP 104768 / NCIMB 8455)) ... Crystal structure of sulfide:quinone oxidoreductase from Acidithiobacillus ferrooxidans in complex with decylubiquinone. *DOI: ... Crystal structure of sulfide:quinone oxidoreductase from Acidithiobacillus ferrooxidans: insights into sulfidotrophic ...
Acidithiobacillus ferrooxidans vs Thiobacillus ferroxidans. About microscopic forms of life, including Bacteria, Archea, ... What is the difference between Acidithiobacillus ferrooxidans versus Thiobacillus ferroxidans? i have been having trouble in ... http://www.bacterio.cict.fr/a/acidithiobacillus.html. Last edited by canalon on Tue Oct 10, 2006 5:25 pm, edited 1 time in ...
Find out information about Acidithiobacillus. An aerobic rod-shaped microorganism that derives its energy from the oxidation of ... various sulfide minerals and soluble ferrous ion ; it thrives in acidic... Explanation of Acidithiobacillus ... redirected from Acidithiobacillus). Also found in: Wikipedia. Thiobacillus ferrooxidans. [‚thī·ō·bə‚sil·əs fə′räk·sə‚danz] ( ... Effectiveness of sulfur with Acidithiobacillus and gypsum in chemical attributes of a Brazilian sodic soil.. Initial growth of ...
Acidithiobacillus ferrooxidans is a Gram-negative bacterium that obtains energy by oxidizing Fe2+ or reduced sulfur compounds. ... Acidithiobacillus ferrooxidans is a Gram-negative bacterium that obtains energy by oxidizing Fe2+ or reduced sulfur compounds. ... Keywords: walnut shell powder; acid mine drainage; Acidithiobacillus ferrooxidans; rus operon walnut shell powder; acid mine ... Use of Walnut Shell Powder to Inhibit Expression of Fe2+-Oxidizing Genes of Acidithiobacillus Ferrooxidans. Yuhui Li 1. ...
Acidithiobacillus ferrooxidans is a chemolithoautotrophic, mesophilic Gram-negative bacterium able to oxidize ferrous iron, ... Shotgun proteomics study of early biofilm formation process of Acidithiobacillus ferrooxidans ATCC 23270 on pyrite.. [Mario ...
Bacteria Acidithiobacillus ferrooxidans. Reference. Kai T, Nagano T, Fukumoto T, Nakajima M, Takahashi T. Autotrophic growth of ... Acidithiobacillus ferrooxidans by oxidation of molecular hydrogen using a gas-liquid contactor. Bioresour Technol. 2007 Jan98(2 ...
However, a large number of Acidithiobacillus strains and sequence clones have been obtained from a variety of ecological niches ... However, a large number of Acidithiobacillus strains and sequence clones have been obtained from a variety of ecological niches ... Presently, the genus is composed of seven validated species, described between 1922 and 2015: Acidithiobacillus thiooxidans, A ... the 580 16S rRNA gene sequences affiliated to the Acidithiobacillus spp. were collected from public and private databases and ...
Hydrometallurgy Acidithiobacillus ferrooxidans.pdf enviado por Igor no curso de Engenharia Elétrica na UNIARA. Sobre: Valdes_ ... Valdes-2008-Hydrometallurgy Acidithiobacillus ferrooxidans. Igorrow Enviado por: Igor Donini , comentários Arquivado no curso ... Keywords: Acidithiobacillus Bioinformatics Comparative genomics Metabolic integration. Draft genome sequences of ... Comparative genome analysis of Acidithiobacillus ferrooxidans, A. thiooxidans and A. caldus: Insights into their metabolism and ...
Dans cette étude, nous avons étudié une bactérie environnementale, Acidithiobacillus thiooxidans, dans le but de comprendre le ... Analyse du métabolisme du soufre de la bactérie autotrophique acidophile Acidithiobacillus thiooxidans ATCC 19377. Thesis or ... In this study, we cultivate an environmental bacterium, Acidithiobacillus thiooxidans, in an attempt to understand its sulfur ...
Molecular and atomic analysis of uranium complexes formed by three eco-types of Acidithiobacillus ferrooxidans M. Merroun; M. ... Molecular and atomic analysis of uranium complexes formed by three eco-types of Acidithiobacillus ferrooxidans. Biochem Soc ... X-ray was used to conduct a molecular and atomic analysis of the uranium complexes formed by Acidithiobacillus ferrooxidans. ...
Collectively, the findings shed light on the phylogeny and divergent evolution of Acidithiobacillus strains, and provided a ... Using the acidophilic sulfur-oxidizer Acidithiobacillus as a case study, taxonomic reclassifications of many isolates provides ... and the feedbacks of the latter might confer the distinct evolutionary patterns of Acidithiobacillus spp. Varied horizontal ... gene transfer events occurred in different bacterial strains, probably resulting in the expansion of Acidithiobacillus genomes ...
Regulation of a Novel Acidithiobacillus caldus Gene Cluster Involved in Metabolism of Reduced Inorganic Sulfur Compounds Olena ... Acidithiobacillus caldus has been proposed to play a role in the oxidation of reduced inorganic sulfur compounds (RISCs) ... Regulation of a Novel Acidithiobacillus caldus Gene Cluster Involved in Metabolism of Reduced Inorganic Sulfur Compounds ... Regulation of a Novel Acidithiobacillus caldus Gene Cluster Involved in Metabolism of Reduced Inorganic Sulfur Compounds ...
  • Acidithiobacillus is a genus of the Acidithiobacillia in the " Proteobacteria " . (wikipedia.org)
  • Here we present the draft genome sequence of Acidithiobacillus albertensis DSM 14366 T , thereby both filling a long-standing gap in the genomics of the acidithiobacilli, and providing further insight into the understanding of the biology of the non iron-oxidizing members of the Acidithiobacillus genus. (biomedcentral.com)
  • Bonnefoy, V, Grail, BM & Johnson, DB 2018, ' Salt stress-induced loss of iron oxidoreduction activities and reacquisition of that phenotype depend on rus operon transcription in Acidithiobacillus ferridurans ', Applied and Environmental Microbiology , vol. 84, no. 7, e02795-17. (coventry.ac.uk)
  • In experiments with sediments from the Lower Red Eyes coal mine drainage in Pennsylvania, USA, cell-normalized iron oxidation rates were nearly twice as fast in reactors incubated with Ferrovum -dominated sediments as with Acidithiobacillus -dominated sediments ( 7 ). (asm.org)
  • Avdalović J., Beškoski V., Gojgić-Cvijović G., Mattinen M.-L., Stojanović M., Zildžović S., Vrvić M. M. Microbial solubilization of phosphorus from phosphate rock by iron-oxidizing Acidithiobacillus sp. (degruyter.com)