Acid Ceramidase
Galactosylgalactosylglucosylceramidase
Neutral Ceramidase
Ceramidases
Alkaline Ceramidase
Farber Lipogranulomatosis
Ceramides
Sphingosine
Sphingolipids
A class of membrane lipids that have a polar head and two nonpolar tails. They are composed of one molecule of the long-chain amino alcohol sphingosine (4-sphingenine) or one of its derivatives, one molecule of a long-chain acid, a polar head alcohol and sometimes phosphoric acid in diester linkage at the polar head group. (Lehninger et al, Principles of Biochemistry, 2nd ed)
Sphingomyelin Phosphodiesterase
Sphingolipid Activator Proteins
Lysosomal Storage Diseases
Lysophospholipids
Specific and sensitive assay for alkaline and neutral ceramidases involving C12-NBD-ceramide. (1/51)
A fluorescent analogue of ceramide, C12-NBD-ceramide, was found to be hydrolyzed much faster than 14C-labeled ceramide by alkaline ceramidase from Pseudomonas aeruginosa and neutral ceramidase from mouse liver, while this substrate was relatively resistant to acid ceramidase from plasma of the horseshoe crab. The radioactive substrate was used more preferentially by the acid ceramidase. It should be noted that C6-NBD-ceramide, which is usually used for ceramidase assays, was hardly hydrolyzed by any of the enzymes examined, compared to C12-NBD-ceramide. For the alkaline and neutral enzymes, the Vmax and k (Vmax/Km) with C12-NBD-ceramide were much higher than those with 14C-ceramide. In contrast, for the acid enzyme these parameters with C12-NBD-ceramide were less than half those with the radioisotope-labeled substrate. It is noteworthy that the labeling of ceramide with NBD did not itself reduce the Km of the alkaline enzyme, but did that of the neutral enzyme. It was also found that C12-NBD-ceramide was preferentially hydrolyzed by the alkaline and neutral enzymes, but not the acid one, in several mammalian cell lines. This study clearly shows that the attachment of NBD, but not dansyl, increases the susceptibility of ceramide to alkaline and neutral enzyme, and decreases that to acid enzymes. Thus the use of this substrate provides a specific and sensitive assay for alkaline and neutral ceramidases. (+info)Molecular cloning, sequencing, and expression of the gene encoding alkaline ceramidase from Pseudomonas aeruginosa. Cloning of a ceramidase homologue from Mycobacterium tuberculosis. (2/51)
We previously reported the purification and characterization of a novel type of alkaline ceramidase from Pseudomonas aeruginosa strain AN17 (Okino, N., Tani, M., Imayama, S., and Ito, M. (1998) J. Biol. Chem. 273, 14368-14373). Here, we report the molecular cloning, sequencing, and expression of the gene encoding the ceramidase of this strain. Specific oligonucleotide primers were synthesized using the peptide sequences of the purified ceramidase obtained by digestion with lysylendopeptidase and used for polymerase chain reaction. DNA fragments thus amplified were used as probes to clone the gene encoding the ceramidase from a genomic library of strain AN17. The open reading frame of 2,010 nucleotides encoded a polypeptide of 670 amino acids including a signal sequence of 24 residues, 64 residues of which matched the amino acid sequence determined for the purified enzyme. The molecular weight of the mature enzyme was estimated to be 70,767 from the deduced amino acid sequence. Expression of the ceramidase gene in Escherichia coli, resulted in production of a soluble enzyme with the identical N-terminal amino acid sequence. Recombinant ceramidase was purified to homogeneity from the lysate of E. coli cells and confirmed to be identical to the Pseudomonas enzyme in its specificity and other enzymatic properties. No significant sequence similarities were found in other known functional proteins including human acid ceramidase. However, we found a sequence homologous to the ceramidase in hypothetical proteins encoded in Mycobacterium tuberculosis, Dictyostelium discoideum, and Arabidopsis thaliana. The homologue of the ceramidase gene was thus cloned from an M. tuberculosis cosmid and expressed in E. coli, and the gene was demonstrated to encode an alkaline ceramidase. This is the first report for the cloning of an alkaline ceramidase. (+info)Overexpression of acid ceramidase protects from tumor necrosis factor-induced cell death. (3/51)
Tumor necrosis factor (TNF) signals cell death and simultaneously induces generation of ceramide. To evaluate the contribution of ceramide to TNF-dependent cell death, we generated clones of the TNF-sensitive cell line L929 that constitutively overexpress human acid ceramidase (AC). Ceramidase, in concert with sphingosine kinase, metabolizes ceramide to sphingosine-1-phosphate (SPP), an inducer of proliferation. In response to TNF, parental L929 cells display a significant increase in intracellular ceramide correlated with an "atypical apoptosis" characterized by membrane blebbing, DNA fragmentation and degradation of poly(ADP-ribose) polymerase despite a lack of caspase activity. These features are strongly reduced or absent in AC-overexpressing cells. Pharmacological suppression of AC with N-oleoylethanolamine restored the accumulation of intracellular ceramide as well as the sensitivity of the transfectants to TNF, implying that an enhanced metabolization of intracellular ceramide by AC shifts the balance between intracellular ceramide and SPP levels towards cell survival. Correspondingly, inhibition of ceramide production by acid sphingomyelinase also increased survival of TNF-treated L929 cells. (+info)Bcl-2 overexpression prevents apoptosis induced by ceramidase inhibitors in malignant melanoma and HaCaT keratinocytes. (4/51)
We examined the biological effects of the ceramide analogues (1S,2R)-2-N-myristoylamino-1-phenyl-1-propanol (D-e-MAPP) and (1R,2R)-2-N-myristoylamino-1-(4-nitrophenyl)-1,3-propandiol (D-NMAPPD) on human HaCaT keratinocytes and human melanoma cells. We could demonstrate that D-e-MAPP and D-NMAPPD are able to suppress acid ceramidase activity. The elevation of the endogenous level of ceramide is followed by induction of apoptosis and suppression of proliferation in HaCaT keratinocytes. Moreover, we recently identified a group of human melanoma cell populations which are heterogeneously susceptible to C2-ceramide-mediated apoptosis. Studies with these melanoma cells revealed correlation between ceramide-mediated apoptosis and D-NMAPPD-induced apoptosis, confirming the effect of this inhibitor on ceramide signaling in human melanoma cells. These findings suggest ceramidase inhibitors as a potential new therapeutical class of antiproliferative and cytostatic drugs. (+info)The reverse activity of human acid ceramidase. (5/51)
An overexpression system was recently developed to produce and purify recombinant, human acid ceramidase. In addition to ceramide hydrolysis, the purified enzyme was able to catalyze ceramide synthesis using [14C]lauric acid and sphingosine as substrates. Herein we report detailed characterization of this acid ceramidase-associated "reverse activity" and provide evidence that this reaction occurs in situ as well as in vitro. The pH optimum of the reverse reaction was approximately 5.5, as compared with approximately 4.5 for the hydrolysis reaction. Non-ionic detergents and zinc cations inhibited the activity, whereas most other cations were stimulatory. Of note, sphingomyelin also was very inhibitory toward this reaction, whereas the anionic lipids, phosphatidic acid and phosphatidylserine, were stimulatory. Of various sphingosine stereoisomers tested in the reverse reaction, only the natural, D-erythro form could efficiently serve as a substrate. Using D-erythro-sphingosine and lauric acid as substrates, the reaction followed normal Michaelis-Menten kinetics. The Km and Vmax values toward sphingosine were 23.75 microM and 208.3 pmol/microg/h, respectively, whereas for lauric acid they were 73.76 microM and 232.5 pmol/microg/h, respectively. Importantly, the reverse activity was reduced in cell lysates from a Farber disease patient to the same extent as the acid ceramidase activity. Furthermore, when 12-(N-methyl-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)) (NBD)-conjugated lauric acid and sphingosine were added to cultured lymphoblasts from a Farber disease patient in the presence of fumonisin B (1), the conversion to NBD-ceramide was reduced approximately 30% when compared with normal cells. These data provide important new information on human acid ceramidase and further document its central role in sphingolipid metabolism. (+info)Stress-induced apoptosis is impaired in cells with a lysosomal targeting defect but is not affected in cells synthesizing a catalytically inactive cathepsin D. (6/51)
The role of cathepsin D in stress-induced cell death has been investigated by using ovine fibroblasts exhibiting a missense mutation in the active site of cathepsin D. The cathepsin D (lysosomal aspartic protease) deficiency did not protect cells against toxicity induced by doxorubicin and other cytotoxic agents, neither did it protect cells from caspase activation. Moreover, the cathepsin D inhibitor, pepstatin A, did not prevent stress-induced cell death in human fibroblasts or lymphoblasts. The possible role of lysosomal ceramide or sphingosine-mediated activation of cathepsin D in apoptosis was also excluded by using human cells either overexpressing or deficient in acid ceramidase. However, a normal lysosomal function seems to be required for efficient cell death, as indicated by the finding that fibroblasts from patients with mucolipidosis II were partially resistant to staurosporine, sphingosine and TNF-induced apoptosis, suggesting a key role of lysosomes in cell death. (+info)Upregulation of the human alkaline ceramidase 1 and acid ceramidase mediates calcium-induced differentiation of epidermal keratinocytes. (7/51)
Extracellular calcium (Ca2+(o)) potently induces the growth arrest and differentiation of human epidermal keratinocytes (HEKs). We report that Ca2+(o) markedly upregulates the human alkaline ceramidase 1 (haCER1) in HEKs; and its upregulation mediates the Ca2+(o)-induced growth arrest and differentiation of HEKs. haCER1 is the human ortholog of mouse alkaline ceramidase 1 that we previously identified. haCER1 catalyzed the hydrolysis of very long-chain ceramides to generate sphingosine (SPH). This in vitro activity required Ca2+. Ectopic expression of haCER1 in HEKs decreased the levels of D-e-C(24:1)-ceramide and D-e-C(24:0)-ceramide but elevated the levels of both SPH and its phosphate (S1P), whereas RNA interference-mediated knockdown of haCER1 caused the opposite effects on the levels of these sphingolipids in HEKs. Similar to haCER1 overexpression, Ca2+(o) increased the levels of SPH and S1P, and this was attenuated by haCER1 knockdown. haCER1 knockdown also inhibited the Ca2+(o)-induced growth arrest of HEKs and the Ca2+(o)-induced expression of keratin 1 and involucrin in HEKs. In addition, the acid ceramidase (AC) was also upregulated by Ca2+(o); and its knockdown attenuated the Ca2+(o)-induced expression of keratin 1 and involucrin in HEKs. These results strongly suggest that upregulation of haCER1 and AC mediates the Ca2+(o)-induced growth arrest and differentiation of HEKs by generating SPH and S1P. (+info)Population genetic analysis of the N-acylsphingosine amidohydrolase gene associated with mental activity in humans. (8/51)
(+info)
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Spinal muscular atrophy with progressive myoclonic epilepsy
The ASAH1 gene codes for acid ceramidase, an enzyme found in lysosomes. The lysosome breaks down acid ceramidase; the fatty ... The lack of myelin resulting from the lack of acid ceramidase breakdown leads to nerve cell dysfunction.[citation needed] ... Park, Jae-Ho; Schuchman, Edward H. (December 2006). "Acid ceramidase and human disease". Biochimica et Biophysica Acta (BBA) - ... "Acid ceramidase deficiency associated with spinal muscular atrophy with progressive myoclonic epilepsy". Neuromuscular ...
ASAH1
... human acid ceramidase. Catalytic reactions and interactions with acid sphingomyelinase". The Journal of Biological Chemistry. ... Okino N, He X, Gatt S, Sandhoff K, Ito M, Schuchman EH (August 2003). "The reverse activity of human acid ceramidase". The ... Seelan RS, Qian C, Yokomizo A, Bostwick DG, Smith DI, Liu W (October 2000). "Human acid ceramidase is overexpressed but not ... Bär J, Linke T, Ferlinz K, Neumann U, Schuchman EH, Sandhoff K (March 2001). "Molecular analysis of acid ceramidase deficiency ...
Farber disease
... autosomal recessive lysosomal storage disease caused by a deficiency of the acid ceramidase enzyme. Acid ceramidase is ... This gene codes for the acid ceramidase enzyme. Individuals with Farber disease have two copies of this gene that are not ... ISBN 978-0-7216-2921-6. Yu, FPS; Amintas, S; Levade, T; Medin, JA (20 July 2018). "Acid ceramidase deficiency: Farber disease ... Farber disease (also known as Farber's lipogranulomatosis, acid ceramidase deficiency, "Lipogranulomatosis", and ASAH1-related ...
Carmofur
However, carmofur is a highly potent acid ceramidase (AC) inhibitor. Ceramide influences cancer cell survival, growth and death ... "Acid ceramidase and its inhibitors: a de novo drug target and a new class of drugs for killing glioblastoma cancer stem cells ... "Acid ceramidase is a novel drug target for pediatric brain tumors". Oncotarget. 8 (15): 24753-24761. doi:10.18632/oncotarget. ... "Discovery of highly potent acid ceramidase inhibitors with in vitro tumor chemosensitizing activity". Scientific Reports. 3 ( ...
ACER1
"Upregulation of the human alkaline ceramidase 1 and acid ceramidase mediates calcium-induced differentiation of epidermal ... Alkaline ceramidase 1 also known as ACER1 is a ceramidase enzyme which in humans is encoded by the ACER1 gene. ACER1 mediates ... Protein, Nucleic Acid, Enzyme. 47 (4 Suppl): 455-62. PMID 11915342. Houben E, Holleran WM, Yaginuma T, Mao C, Obeid LM, Rogiers ... Ito M, Okino N, Tani M, Mitsutake S, Mori K (Mar 2002). "[Molecular evolution of neutral ceramidase: signalling molecule and ...
IRF8
In chronic myelogenous leukemia (CML), IRF8 regulates acid ceramidase to mediate CML apoptosis. IRF8 is highly expressed in ... "IRF8 regulates acid ceramidase expression to mediate apoptosis and suppresses myelogeneous leukemia". Cancer Res. 71 (8): 2882- ...
Sphingosine-1-phosphate
S1P is formed from ceramide, which is composed of a sphingosine and a fatty acid. Ceramidase, an enzyme primarily present in ... Lipid signaling Lysophosphatidic acid Mendelson, Karen; Evans, Todd; Hla, Timothy (1 January 2014). "Sphingosine 1-phosphate ... October 2000). "Oocyte apoptosis is suppressed by disruption of the acid sphingomyelinase gene or by sphingosine-1-phosphate ... Progress in Nucleic Acid Research and Molecular Biology. 71: 493-511. doi:10.1016/s0079-6603(02)71049-0. ISBN 9780125400718. ...
ASAHL
2005). "Elevation of the level and activity of acid ceramidase in Alzheimer's disease brain". Eur. J. Neurosci. 20 (12): 3489- ... This gene encodes an N-acylethanolamine-hydrolyzing enzyme which is highly similar to acid ceramidase. Multiple transcript ... "Entrez Gene: ASAHL N-acylsphingosine amidohydrolase (acid ceramidase)-like". Human NAAA genome location and NAAA gene details ... Schulze H, Schepers U, Sandhoff K (2008). "Overexpression and mass spectrometry analysis of mature human acid ceramidase". Biol ...
Ceramide
... can be further hydrolyzed by acid ceramidase to form sphingosine and a free fatty acid, both of which are able to ... A ceramide is composed of sphingosine and a fatty acid. Ceramides are found in high concentrations within the cell membrane of ... Rotolo JA, Zhang J, Donepudi M, Lee H, Fuks Z, Kolesnick R (2005). "Caspase-dependent and -independent activation of acid ... Anandamide Ceramidase inhibitors Chemotherapeutic agents Fas ligand Endotoxin Homocysteine Heat Hydroxypalmitoyl Sphinganine ...
Hugo Moser (scientist)
... and they identified acid ceramidase as the enzyme deficient in Farber's disease. In 1964, Moser returned to the Joseph P. ... Together, they organized a symposium at Kennedy Krieger where Bill Rizzo presented data that showed that adding oleic acid (C- ... Yasuo Kishimoto, who had helped Kuni Suzuki make the discovery of elevated very long chain fatty acids in Adrenoleukodystrophy ... Odone made an independent intellectual computation - namely that the use of erucic acid, which is C-22:1 (C-22 monounsaturated ...
Daniele Piomelli
Fatty Acid Amide Hydrolase inhibitors), PEA (N-Acylethanolamide Acid Amidase inhibitors), and ceramide (Acid Ceramidase ... "Discovery of highly potent acid ceramidase inhibitors with in vitro tumor chemosensitizing activity". Science Reports 3:1035. ... "Selective N-acylethanolamine-hydrolyzing acid amidase inhibition reveals a key role for endogenous palmitoylethanolamide in ...
ASAH
... acid ceramidase), an enzymatic product of ASAH1, gene mutated in Farber disease Australian Society for Asian Humanities This ...
Chromosome 8
... acid ceramidase) 1 ASPH: encoding enzyme Aspartyl/asparaginyl beta-hydroxylase AZIN1: encoding protein Antizyme inhibitor 1 ... fatty acid binding protein 9, testis FAM167A: family with sequence similarity 167, member A FAM203B: family with sequence ...
Lysosomal storage disease
... sialic acid storage disease Infantile free sialic acid storage disease Glycogen storage diseases Type II Pompe disease Type IIb ... Lysosomal storage diseases include: Sphingolipidoses Ceramidase Farber disease Krabbe disease Infantile onset Late onset ... Cystinosis is an lysosomal storage disease characterized by the abnormal accumulation of the amino acid cystine. Alternatively ... Chronic hexosaminidase A deficiency Glucocerebroside Gaucher disease Type I Type II Type III Sphingomyelinase Lysosomal acid ...
N-Acylethanolamine
... ceramidase converts ceramide to sphingosine) and blocks the effects of TNF- and arachidonic acid on intracellular Ca2+ ... to eicosatetraenoic acid (ETA: C20H32O2; 20:4-n3; omega-3 Arachidonic acid) and arachidonic acid (AA: C20H32O2; 20:4-n6) to ... and heptadecanoic acid (C17H34O2; 17:0), elevated palmitoleic acid (POA; C16H30O2; 16:1, n-7), a conjugate acid of a ... where n-7 fatty acids are precursors for the production of omega-4 fatty acids like palmitolinoleic acid (16:2), and a ...
List of EC numbers (EC 3)
The activity may be that of an acid phosphatase EC 3.1.3.31: The activity may be that of an acid phosphatase EC 3.1.3.32: ... ceramidase EC 3.5.1.24: choloylglycine hydrolase EC 3.5.1.25: N-acetylglucosamine-6-phosphate deacetylase EC 3.5.1.26: N4-(β-N- ... ABC-type polar-amino-acid transporter EC 3.6.3.22: Now EC 7.4.2.2, ABC-type nonpolar-amino-acid transporter EC 3.6.3.23: Now EC ... EC 3.1.2.25: phenylacetyl-CoA hydrolase EC 3.1.2.26: Now EC 2.8.3.25, bile acid CoA transferase EC 3.1.2.27: choloyl-CoA ...
Ceramidase
Presently, 7 human ceramidases encoded by 7 distinct genes have been cloned: acid ceramidase (ASAH1) - cell survival neutral ... Ceramidase at the US National Library of Medicine Medical Subject Headings (MeSH) EC 3.5.1.23 Portal: Biology v t e (EC 3.5.1, ... Human neutral ceramidase (nCDase) is an enzyme that plays a critical role in colon cancer and there are currently no potent or ... Ceramidase (EC 3.5.1.23, acylsphingosine deacylase, glycosphingolipid ceramide deacylase) is an enzyme which cleaves fatty ...
N-acylethanolamine acid amide hydrolase
NAAA is homologous to acid ceramidase (HUGO gene symbol: ASAH1), sharing 30% sequence identity at the amino acid level in ... its structural similarity to the more familiar acid ceramidase (AC) and functional similarity to fatty acid amide hydrolase ( ... NAAA's primary structure is nearly identical to that of acid ceramidase (AC), whose only difference is the substitution of Leu ... a novel member of the choloylglycine hydrolase family with structural and functional similarity to acid ceramidase". The ...
ASAH2
"Cooperative prosurvival activity by ERK and Akt in human alveolar macrophages is dependent on high levels of acid ceramidase ... Neutral ceramidase is an enzyme that in humans is encoded by the ASAH2 gene. GRCh38: Ensembl release 89: ENSG00000188611 - ... 2004). "Neutral ceramidase gene: role in regulating ceramide-induced apoptosis". Gene. 315: 113-22. doi:10.1016/S0378-1119(03) ... 2006). "Golgi alkaline ceramidase regulates cell proliferation and survival by controlling levels of sphingosine and S1P". ...
Sphingolipid
A brief, very comprehensible review is given in Gunstone, F. (1996) Fatty Acid and Lipid Chemistry, pp 43-44. Blackie Academic ... Finally, ceramide may be broken down by a ceramidase to form sphingosine. Sphingosine may be phosphorylated to form sphingosine ... Gangliosides have at least three sugars, one of which must be sialic acid. Inositol-containing ceramides, which are derived ... such as a fatty acid.[citation needed] Simple sphingolipids, which include the sphingoid bases and ceramides, make up the early ...
Lipid signaling
"Docosahexaenoic acid-derived fatty acid esters of hydroxy fatty acids (FAHFAs) with anti-inflammatory properties". Diabetes. 65 ... Sphingosine (Sph) is formed by the action of ceramidase (CDase) enzymes on ceramide in the lysosome. Sph can also be formed in ... Palmitic acid esters of hydroxy-stearic acids (PAHSAs) are among the most bioactive members able to activate G-protein coupled ... Docosahexaenoic acid ester of hydroxy-linoleic acid (DHAHLA) exert anti-inflammatory and pro-resolving properties. ...
Fumonisin B1
Folic acid uptake was dramatically inhibited. Treatment after exposure with folic acid reduced neural tube defects by 50-65%. ... de formation via de novo sphinganine and fatty acyl-CoA and via sphingosine produced by the breakdown of ceramide by ceramidase ... However, when the tricarbillic acid groups are removed from FB1 by hydrolysis, the resulting product (aminopentol, AP1) doesn't ... whereas the negatively charged tricarbyllic acid groups may interact with the fatty acyl-CoA binding site. Because FB1 also ...
Shapiro Senapathy algorithm
Roca, X. (2003-11-01). "Intrinsic differences between authentic and cryptic 5' splice sites". Nucleic Acids Research. 31 (21): ... "A neutral ceramidase, NlnCDase, is involved in the stress responses of brown planthopper, Nilaparvata lugens (Stål)". ... Nucleic Acids Res. 18 (10): 3015-9. doi:10.1093/nar/18.10.3015. PMC 330832. PMID 2349097. Senapathy, P. (1986). "Origin of ... Nucleic Acids Research. 37 (9): e67. doi:10.1093/nar/gkp215. ISSN 1362-4962. PMC 2685110. PMID 19339519. "Splice-Site Analyzer ...
ENPP7
Unlike acid and neutral SMases in the intestinal tract that are rapidly inactivated by pancreatic trypsin, alk-SMase is ... neutral ceramidase, and sphingolipid metabolites". Pediatr. Res. 61 (1): 61-6. doi:10.1203/01.pdr.0000250534.92934.c2. PMID ... Besides, ursodeoxycholic acid and probiotic VSL#3 may stimulate the expression of ENPP7 in the intestine. GRCh38: Ensembl ... Liu F, Cheng Y, Wu J, Tauschel HD, Duan RD (April 2006). "Ursodeoxycholic acid differentially affects three types of ...
Lipid Storage Disorders Treatment & Management: Medical Care, Consultations, Diet
In vivo delivery of human acid ceramidase via cord blood transplantation and direct injection of lentivirus as novel treatment ... Lysosomal acid alpha-glucosidase consists of four different peptides processed from a single chain precursor. J Biol Chem. 2005 ... Lysosomal Acid Lipase Deficiency: Therapeutic Options. Drug Des Devel Ther. 2020. 14:591-601. [QxMD MEDLINE Link]. ... Acid sphingomyelinase deficiency: prevalence and characterization of an intermediate phenotype of Niemann-Pick disease. J ...
Lysosomal Storage Disease: Overview, Classification of Lysosomal Storage Diseases, Glycogen Storage Disease Type II
Sialic acid storage disease (Salla disease; sialic acid transporter deficiency): Clinical features include hypotonia, ... Farber disease, disseminated lipogranulomatosis (ceramidase deficiency): Farber disease starts to manifest in infancy as a ... Glycogen storage disease type II, or acid alpha-glucosidase (acid maltase) deficiency, is an inherited disorder of glycogen ... Two major presentations are (1) infantile acid maltase disease, or Pompe disease, and (2) slowly progressive acid maltase ...
ASAH1 gene: MedlinePlus Genetics
The ASAH1 gene provides instructions for making an enzyme called acid ceramidase. Learn about this gene and related health ... they travel to lysosomes where acid ceramidase breaks them down into a fat called sphingosine and a fatty acid. These two ... The ASAH1 gene variants that cause SMA-PME result in a reduction of acid ceramidase activity to a level less than one-third of ... The reduction in acid ceramidase activity associated with SMA-PME is less than what occurs in another condition called Farber ...
Spinal muscular atrophy with progressive myoclonic epilepsy: MedlinePlus Genetics
Within lysosomes, acid ceramidase breaks down fats called ceramides into a fat called sphingosine and a fatty acid. These two ... Acid ceramidase deficiency: Farber disease and SMA-PME. Orphanet J Rare Dis. 2018 Jul 20;13(1):121. doi: 10.1186/s13023-018- ... ASAH1 gene variants that cause SMA-PME result in a reduction of acid ceramidase activity to a level less than one-third of ... This gene provides instructions for making an enzyme called acid ceramidase. This enzyme is found in lysosomes. , which are ...
Farber lipogranulomatosis: MedlinePlus Genetics
Acid ceramidase breaks down fats called ceramides into a fat called sphingosine and a fatty acid. These two breakdown products ... Molecular basis of acid ceramidase deficiency in a neonatal form of Farber disease: identification of the first large deletion ... Acid ceramidase deficiency: Farber disease and SMA-PME. Orphanet J Rare Dis. 2018 Jul 20;13(1):121. doi: 10.1186/s13023-018- ... Variants in the ASAH1 gene lead to severe reduction in acid ceramidase, typically to below 10 percent of normal. As a result, ...
Lysosomal Storage Disease: Overview, Classification of Lysosomal Storage Diseases, Glycogen Storage Disease Type II
Sialic acid storage disease (Salla disease; sialic acid transporter deficiency): Clinical features include hypotonia, ... Farber disease, disseminated lipogranulomatosis (ceramidase deficiency): Farber disease starts to manifest in infancy as a ... Glycogen storage disease type II, or acid alpha-glucosidase (acid maltase) deficiency, is an inherited disorder of glycogen ... Two major presentations are (1) infantile acid maltase disease, or Pompe disease, and (2) slowly progressive acid maltase ...
Discovery of highly potent acid ceramidase inhibitors with in vitro tumor chemosensitizing activity | Scientific Reports
The expression of acid ceramidase (AC) - a cysteine amidase that hydrolyses the proapoptotic lipid ceramide - is abnormally ... Recombinant acid ceramidase expression. Rat AC was cloned from a brain cDNA library using primers based on the sequence ... Acid ceramidase controls apoptosis and increases autophagy in human melanoma cells treated with doxorubicin *Michele Lai ... Acid ceramidase (AC, also known as N-acylsphingosine amidohydrolase-1, ASAH-1) is a cysteine amidase that catalyzes the ...
Farber lipogranulomatosis: MedlinePlus Genetics
Acid ceramidase breaks down fats called ceramides into a fat called sphingosine and a fatty acid. These two breakdown products ... Molecular basis of acid ceramidase deficiency in a neonatal form of Farber disease: identification of the first large deletion ... Acid ceramidase deficiency: Farber disease and SMA-PME. Orphanet J Rare Dis. 2018 Jul 20;13(1):121. doi: 10.1186/s13023-018- ... Variants in the ASAH1 gene lead to severe reduction in acid ceramidase, typically to below 10 percent of normal. As a result, ...
Spinal muscular atrophy with progressive myoclonic epilepsy - Wikipedia
The ASAH1 gene codes for acid ceramidase, an enzyme found in lysosomes. The lysosome breaks down acid ceramidase; the fatty ... The lack of myelin resulting from the lack of acid ceramidase breakdown leads to nerve cell dysfunction.[citation needed] ... Park, Jae-Ho; Schuchman, Edward H. (December 2006). "Acid ceramidase and human disease". Biochimica et Biophysica Acta (BBA) - ... "Acid ceramidase deficiency associated with spinal muscular atrophy with progressive myoclonic epilepsy". Neuromuscular ...
Publications - Graduiertenkolleg 2581
The acid ceramidase/ceramide axis controls parasitemia in Plasmodium yoelii-infected mice by regulating erythropoiesis ... The acid ceramidase/ceramide axis controls parasitemia in Plasmodium yoelii-infected mice by regulating erythropoiesis ... Staphylococcus aureus a-Toxin Induces Acid Sphingomyelinase Release From a Human Endothelial Cell Line ... Inhibition of acid sphingomyelinase increases regulatory T cells in humans. T. Wiese, F. Dennstädt, C. Hollmann, S. Stonawski, ...
Lipid Storage Disorders Treatment & Management: Medical Care, Consultations, Diet
In vivo delivery of human acid ceramidase via cord blood transplantation and direct injection of lentivirus as novel treatment ... Lysosomal acid alpha-glucosidase consists of four different peptides processed from a single chain precursor. J Biol Chem. 2005 ... Lysosomal Acid Lipase Deficiency: Therapeutic Options. Drug Des Devel Ther. 2020. 14:591-601. [QxMD MEDLINE Link]. ... Acid sphingomyelinase deficiency: prevalence and characterization of an intermediate phenotype of Niemann-Pick disease. J ...
Frontiers | Rationale for Targeting Deregulated Metabolic Pathways as a Therapeutic Strategy in Acute Myeloid Leukemia
Acid ceramidase is upregulated in AML and represents a novel therapeutic target. Oncotarget. (2016) 7:83208-22. doi: 10.18632/ ... The overexpression of acid ceramidase (AC) which catalyzes the breakdown of ceramide to sphingosine, the precursor for S1P, ... fatty acid binding protein-4; FAs, fatty acids; FLT3-ITD, FLT3 receptor with internal tandem duplication mutation; FRU, ... Targeting the Amino-Acid Addiction of AML Cells. With glucose, glutamine is the main nutrient source for the growth/survival of ...
Microbiology and Infectious Disease | eLife
Lipid Pathway Dysfunction in Patients With Parkinson's Disease
Dengue vascular leakage is augmented by mast cell degranulation mediated by immunoglobulin Fcγ receptors | eLife
Oleylethanolamide Supplier | CAS 111-58-0 | Tocris Bioscience
Calignano et al (2001) Antinociceptive activity of the endogenous fatty acid amide, palmitylethanolamide. Eur.J.Pharmacol. 419 ... Coroneos et al (1995) Differential regulation of sphingomyelinase and ceramidase activities by growth factors and cytokines. J. ... Ceramidase, inhibitors, inhibits, Endogenous, GPR119, receptor, Peroxisome, Proliferator-activating, Receptors, PPAR, ... α and is also a ceramidase inhibitor. Also endogenous agonist at the GPR119 receptor. ...
Melanoma treatment could benefit from nanotech drug delivery
Turner S[au] - Search Results - PubMed
Find Research outputs
- Yonsei University
Code System Concept
SMART: calpain III domain annotation
... acid ceramidase) an arginine is the general base. Cysteine peptidases can be grouped into fourteen different clans, with ... 2017) Nucleic Acids Res doi: 10.1093/nar/gkx922. Letunic et al. (2020) Nucleic Acids Res doi: 10.1093/nar/gkaa937 ... They are inhibited by thiol chelators such as iodoacetate, iodoacetic acid, N -ethylmaleimide or p -chloromercuribenzoate. ... An 18-amino acid extended sequence linking domain III to domain IV; ...
Alkaline ceramidase 2 regulates beta;1 integrin maturation and cell adhesion - Fingerprint - United Arab Emirates...
Agriculture and Life Sciences, College of - Grants - University of Arizona
Sphingolipids in neuroinflammation: a potential target for diagnosis and therapy
... the acetylated COX2 produces SPMs from arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid (36, 37). SPMs are ... Ceramide deacylation by ceramidase is the only pathway to produce sphingosine, and sphingosine can also be reversed to ceramide ... When COX2 is acetylated by acetylsalicylic acid (ASA, aspirin) (34, 35), ... ceramidase, and sphingosine kinase (SphK), in the biosynthesis and catabolism of sphingolipids (2). Furthermore, sphingolipids ...
SMART: Pfam domain OTU
... acid ceramidase) an arginine is the general base. Cysteine peptidases can be grouped into fourteen different clans, with ... 2017) Nucleic Acids Res doi: 10.1093/nar/gkx922. Letunic et al. (2020) Nucleic Acids Res doi: 10.1093/nar/gkaa937 ... They are inhibited by thiol chelators such as iodoacetate, iodoacetic acid, N -ethylmaleimide or p -chloromercuribenzoate. ... usually asparagine or aspartic acid, to orientate and activate the imidazolium ring. In only one family of cysteine peptidases ...
Lipids in Health and Disease | Articles
Sciadonic acid (SA) is an anti-inflammatory fatty acid displacing arachidonic acid (ARA) from specific phospholipid pools, thus ... AdipoR1 and AdipoR2 (AdipoRs) are plasma membrane proteins often considered to act as adiponectin receptors with a ceramidase ... Omega-3 fatty acids coordinate glucose and lipid metabolism in diabetic patients Omega 3 polyunsaturated fatty acids (n-3 PUFA ... Dietary fatty acids intake affects the composition of erythrocyte fatty acids, which is strongly correlated with glycolipid ...
Publications - DE VIVO LAB - MOLECULAR MODELING & DRUG DISCOVERY
Patent filed on new anticancer compounds targeting the acid ceramidase enzyme. "Acid Ceramidase inhibitors, and their use as ... "Benzoxazolone derivatives as Acid Ceramidase inhibitors, and their use as medicaments". Piomelli D., Pagliuca C., Pizzirani D ... "Benzoxazolone-Carboxamides: Potent systemically active inhibitors of intracellular Acid Ceramidase". Angew. Chem. Int. Ed. Engl ... potent and chemically stable inhibitors of acid ceramidase in melanoma cells". J. Med. Chem., 2017, 60 (13), pp 5800-5815 (Link ...
ABCA2 modulates the expression of the Alzheimer's disease gene, amyloid precursor protein (APP).
Pharmacological inhibition of ceramidase activity or activation PKC activity with 12-myristate 13-acetate (PMA) or ... ABCA2 overexpression increased in vitro alkaline and acid ceramidase activity. Sphingosine is a physiological inhibitor of ... ABCA2 overexpression increased in vitro alkaline and acid ceramidase activity. Sphingosine is a physiological inhibitor of ... Pharmacological inhibition of ceramidase activity or activation PKC activity with 12-myristate 13-acetate (PMA) or ...
Lysosomal Disorders - CHEMICAL BIOLOGY
Acid ceramidase. Ceramide. Nodular swelling around joints, hypotonia, visceromegaly, CNS involvement, angiokeratoma ... where D is aspartic acid, E is glutamic acid, L is leucine, I is isoleucine, and X is any amino acid] are found within the ... Sorting signals that are either tyrosine-based (NPXY, YXX: where N is asparagine, P is proline, and X is any amino acid) or ... 1) or transit to the late endosome where ligands are dissociated from the receptors by the acid pH environment. For lysosomal ...
Cell Biology - Research output - University of Texas Southwestern Medical Center
SMART: Pfam domain UCH
... acid ceramidase) an arginine is the general base. Cysteine peptidases can be grouped into fourteen different clans, with ... 2017) Nucleic Acids Res doi: 10.1093/nar/gkx922. Letunic et al. (2020) Nucleic Acids Res doi: 10.1093/nar/gkaa937 ... They are inhibited by thiol chelators such as iodoacetate, iodoacetic acid, N -ethylmaleimide or p -chloromercuribenzoate. ... usually asparagine or aspartic acid, to orientate and activate the imidazolium ring. In only one family of cysteine peptidases ...
Find Research outputs
- Yonsei University
Achievements 2014 - 2015 | Renaissance School of Medicine at Stony Brook University
Respiratory manifestations in patients with inherited metabolic diseases | European Respiratory Society
In patients with Farber lipogranulomatosis, which is due to a deficiency of acid ceramidase activity that results in the ... Farbers lipogranulomatosis: ceramidase deficiency and prolonged survival in three relatives. Johns Hopkins Med J 1980; 147: ... Disorders of amino acids transport and metabolism. Lysinuric protein intolerance. Lysinuric protein intolerance, an autosomal ... Recombinant human acid alpha-glucosidase (rhGAA) in adult patients with severe respiratory failure due to Pompe disease. ...
Genatlas sheet
AlkalineDeficiencyCeramideSphingomyelinaseInhibitionInhibitorSphingosineMetabolismNucleic AciEnzymeASAH1EnzymesBile AcidsFree fattyRabbit PolyclonalRegulatesAmino acidsFatty acid synTumorReceptorCysteineMitochondrialDysfunctionActivityLysosomesGlutamic acidArachidonic acidPathwayMyelinProteinLipidsAcidicCeramidesProteinsDeoxycholicInfectionHumanMetabolicCompartmentsCellsSerumActivation
Alkaline3
- ABCA2 overexpression increased in vitro alkaline and acid ceramidase activity. (growkudos.com)
- alkaline ceramidase 3 [Source:HGNC S. (gsea-msigdb.org)
- The present idea which mPRs are generally alkaline ceramidases and also mediate sphingolipid signaling isn't sustained by test Selleck Tariquidar data in which TNF alpha does not situation to be able to mPRs overexpressed in human cells knowning that exogenous sphingomyelinase will be unsuccessful throughout mimicking progestin actions by way of mPRs to be able to stimulate meiotic growth involving fish oocytes. (screeninglibraries.com)
Deficiency5
- Alves MQ, Le Trionnaire E, Ribeiro I, Carpentier S, Harzer K, Levade T, Ribeiro MG. Molecular basis of acid ceramidase deficiency in a neonatal form of Farber disease: identification of the first large deletion in ASAH1 gene. (medlineplus.gov)
- Bar J, Linke T, Ferlinz K, Neumann U, Schuchman EH, Sandhoff K. Molecular analysis of acid ceramidase deficiency in patients with Farber disease. (medlineplus.gov)
- Acid ceramidase deficiency: Farber disease and SMA-PME. (capsulehealth.one)
- Farber disease (FD) is an extremely rare autosomal recessive disorder caused by the deficiency of lysosomal acid ceramidase. (ebsco.com)
- Neutral ceramidase deficiency protects against cisplatin-induced acute kidney injury. (musc.edu)
Ceramide3
- The expression of acid ceramidase (AC) - a cysteine amidase that hydrolyses the proapoptotic lipid ceramide - is abnormally high in several human tumors, which is suggestive of a role in chemoresistance. (nature.com)
- Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. (utsouthwestern.edu)
- Barron K, Ogretmen B, Krupenko N. Ceramide synthase 6 mediates sex-specific metabolic response to dietary folic acid in mice. (musc.edu)
Sphingomyelinase1
- Decreased activity of blood acid sphingomyelinase in the course of multiple myeloma. (edu.pl)
Inhibition5
- Pharmacological inhibition of ceramidase activity or activation PKC activity with 12-myristate 13-acetate (PMA) or diacylglycerol (DAG) decreased endogenous APP mRNA levels in ABCA2 overexpressing cells. (growkudos.com)
- We now show OSI-420 that inhibition of the fatty acid synthase (Fas2) results in a hypersensitivity to serum indicating that yeast cell survival and replication in serum medium or in vivo is dependent on Fas2. (immune-source.com)
- Inhibition of enzymes in this pathway such as fatty acid synthase and fatty acid desaturase impedes yeast cell growth unless appropriate exogenous fatty acids are provided [1]-[3]. (immune-source.com)
- Thus inhibition of a single enzyme in the fatty acid synthesis pathway can result in profoundly altered physiological phenotypes and may impact virulence in pathogenic yeasts. (immune-source.com)
- Western blotting results indicated that the inhibition of SOX2 in bile acid-treated AGS cells is partially rescued in miR-21 knockdown AGS cells. (medchemexpress.com)
Inhibitor5
- Induces satiety through activation of PPAR α and is also a ceramidase inhibitor. (tocris.com)
- 2006) New insights on the use of desipramine as an inhibitor for acid ceramidase. (sternekessler.com)
- For example isoniazid is a fatty acid synthesis inhibitor that is used to treat tuberculosis [4] [5]. (immune-source.com)
- Gluconate sodium (D-Gluconic acid sodium salt) is a corrosion and scale inhibitor of ordinary steel in simulated cooling water. (medchemexpress.com)
- Vesicles containing acid solution lipase, which hydrolyzes LDL cholesterol esters to free of charge cholesterol, colocalized with FLOT2 and encircled inclusions, as the acid lipase inhibitor orlistat inhibited replication. (secretion.org)
Sphingosine1
- Acid ceramidase breaks down fats called ceramides into a fat called sphingosine and a fatty acid. (medlineplus.gov)
Metabolism3
- Here, he worked on covalent small-molecule inhibitors of acid ceramidase and related enzymes involved in lipid metabolism and signalling. (chemicalprobes.org)
- Deoxycholic acid (cholanoic acid) sodium hydrate,a bile acid, is a by-product of intestinal metabolism, that activates the G protein-coupled bile acid receptor TGR5 . (medchemexpress.com)
- Ribosomal protein-Mdm2-p53 pathway coordinates nutrient stress with lipid metabolism by regulating MCD and promoting fatty acid oxidation , Proceedings of the National Academy of Sciences , vol. 11 , issue. (inserm.fr)
Nucleic Aci1
- 1997 Nucleic acids Res. (lbl.gov)
Enzyme3
- The ASAH1 gene provides instructions for making an enzyme called acid ceramidase. (medlineplus.gov)
- The ASAH1 gene codes for acid ceramidase, an enzyme found in lysosomes. (wikipedia.org)
- Meanwhile, Medical News Today recently learned about a new class of drugs that may boost the antitumor effects of some melanoma treatments by reducing the activity of an enzyme called acid ceramidase. (medicalnewstoday.com)
ASAH11
- Variants in the ASAH1 gene lead to severe reduction in acid ceramidase, typically to below 10 percent of normal. (medlineplus.gov)
Enzymes1
- Based on the model, histone methyltransferase enzymes (HMTases) methylate the Ertugliflozin L-pyroglutamic acid histone H3 at lysine 9 (H3K9me), creating selective binding sites for itself as well as the chromodomain of Horsepower1a. (rfemmr.org)
Bile Acids2
- Deoxycholic acid sodium hydrate (DCA) (100 μM) induces the production of gastric cancer cell line MGC803 resistant to acidified bile acids and enhances their survival and proliferation activity under bile acid stress [2] . (medchemexpress.com)
- Acidified bile acids enhance tumor progression and telomerase activity of gastric cancer in micedependent on c-Myc expression. (medchemexpress.com)
Free fatty1
- Lipotoxicity is a consequence of a high-fat diet (HFD), resulting in elevated circulating free fatty acids and can lead to insulin resistance and metabolic dysfunction [ 6 ]. (biomedcentral.com)
Rabbit Polyclonal1
- Gas-filled MBs have already been utilized as ultrasound comparison agencies as well as Rabbit Polyclonal to Syntaxin 1A (phospho-Ser14) for various other applications medically, such as for example providing genes or medications into cells or for breaching the Azelaic acid bloodCbrain hurdle [18, 19]. (cell-signaling-pathways.com)
Regulates2
- In yeasts the de novo fatty acid synthesis pathway produces and regulates essential fatty acid species such as saturated (SFA) and unsaturated (UFA) fatty acids that are required for generation and maintenance of cell membranes. (immune-source.com)
- ROS-mediated p53 induction of Lpin1 regulates fatty acid oxidation in response to nutritional stress , Molecular Cell , vol. 44 , issue. (inserm.fr)
Amino acids1
- Thanks to its potent ability to produce amylase and protease that allows it to decompose proteins and different starches into sugars and amino acids (Watarai et al. (springeropen.com)
Fatty acid syn1
- However little is known concerning the pathobiology of fatty acid synthase (Fas2) is essential for viability in the lack of exogenous essential fatty OSI-420 acids and. (immune-source.com)
Tumor1
- Understanding that the Compact Azelaic acid disc44+ is certainly a utilized cancer-stem-cell biomarker frequently, our targeted biotin-MBs is actually a powerful Azelaic acid tool to kind cancers stem cells from dissected tumor tissues for make use of in preclinical tests and clinical studies. (cell-signaling-pathways.com)
Receptor1
- Six months of EE led to increases in muscle lipoprotein lipase, peroxisome proliferator-activated receptor-gamma coactivator-1alpha, carnitine palmitoyltransferase-1 beta, diacylglycerol acyltransferase-1, and acid ceramidase mRNA in women, but not men. (duke.edu)
Cysteine2
- Hydrolysis involves usually a catalytic triad consisting of the thiol group of the cysteine, the imidazolium ring of a histidine, and a third residue, usually asparagine or aspartic acid, to orientate and activate the imidazolium ring. (embl-heidelberg.de)
- In only one family of cysteine peptidases, is the role of the general base assigned to a residue other than a histidine: in peptidases from family C89 (acid ceramidase) an arginine is the general base. (embl-heidelberg.de)
Mitochondrial1
- To our knowledge this is the first study to critically define a link between cytosolic fatty acid synthesis and mitochondrial function in response to serum stress in is a human opportunistic pathogen associated with significant morbidity and mortality especially in immunocompromised individuals such as premature low-birthweight neonates. (immune-source.com)
Dysfunction1
- The lack of myelin resulting from the lack of acid ceramidase breakdown leads to nerve cell dysfunction. (wikipedia.org)
Activity3
- It is unclear whether the level of acid ceramidase activity is related to the severity of the disorder. (medlineplus.gov)
- Host cell acid ceramidase activity contributes to the regulation of erythrocyte maturation and thereby affects the frequency of target cells for rodent Plasmodium yoelii parasites. (elifesciences.org)
- Deoxycholic acid sodium hydrate (DCA) (100 μM)-induced resistant cells shows altered morphology, significantly elevated telomerase activity, better cell viability and reduces apoptosis compared to normal MGC803 cells [2] . (medchemexpress.com)
Lysosomes1
- From early observations, De Duve defined lysosomes as cytoplasmic particles that were associated with a range of acid hydrolases (7). (schoolbag.info)
Glutamic acid1
- When this occurs, CRAF remains constitutively activated which leads to MEK/ERK activation.8C10 The most common mutation, accounting for 70C88% of all mutations, is a substitution of glutamic acid for valine at amino acid 600 (V600E).7,11 Other mutations in occur less frequently and include V600K, V600R, V600M, non-V600 alterations and fusions. (globaltechbiz.com)
Arachidonic acid2
- Sciadonic acid (SA) is an anti-inflammatory fatty acid displacing arachidonic acid (ARA) from specific phospholipid pools, thus modulating downstream pro-inflammatory lipid mediators. (biomedcentral.com)
- First, phospholipase A2 promotes the cleavage of phospholipids into arachidonic acid (AA), which becomes substrate of the COX-1/2 to produce the unstable endoperoxide metabolite PGH2. (biotechpolicy.org)
Pathway2
- Although the potential of exploiting the fatty acid biosynthesis pathway for targeting microbial infections is still in argument these studies suggest the importance of evaluating the efficacy of drugs in more complex media such as serum. (immune-source.com)
- We have recently demonstrated that modulation of the sphingolipid biosynthetic pathway such as targeted expression of ceramidase, rescues degeneration in certain photoreceptor mutants. (cancer.gov)
Myelin3
- the fatty acid component is then used to produce myelin. (wikipedia.org)
- GALC Null oligodendrocytes produce psychosine from GalCer via acid ceramidase, undergo diffuse loss of myelin, and contribute to secondary axonal degeneration. (cahrr.org)
- a) Luxol-Fast-Blue, periodic acid schiff (PAS) staining of the cerebellar white matter from a GLD patient shows profound demyelination (absence of blue-stained myelin), neurodegeneration (low density of purple stained axons, arrows) and Globoid cells filled with PAS positive glycolipids storage (arrowhead). (cahrr.org)
Protein2
- Among these, protein tyrosine nitration and polyunsaturated fatty acid nitration are the most studied types to date. (frontiersin.org)
- fatty acid binding protein 5 [Source. (gsea-msigdb.org)
Lipids1
- Our prior studies have indicated that effectively utilizes fatty acids/lipids for growth and virulence. (immune-source.com)
Acidic1
- The definitive description of a lysosome (16) includes a membrane-bound organelle compartment that is acidic and contains a range of mature acid hydrolases (e.g., proteases and glycosidases). (schoolbag.info)
Ceramides1
- These are ceramides bearing a long chain fatty acid. (foodb.ca)
Proteins1
- In Horsepower1a mutants just the PEP immunopattern is certainly unusual, whereas in PEP mutants the immunopatterns Ertugliflozin L-pyroglutamic acid of most various other proteins are regular. (rfemmr.org)
Deoxycholic2
- Deoxycholic acid sodium hydrate purchased from MCE. (medchemexpress.com)
- PGE2 has also been shown to induce colon carcinogenesis in the presence of bile acid, deoxycholic acid in male Sprague-Dawley rats [9], and to enhance azoxymethane-induced colon tumors in mice by increasing cellular proliferation and inhibiting apoptosis [10]. (biotechpolicy.org)
Infection1
- Fatty acid synthesis pathways have been considered as targets to combat bacterial infection. (immune-source.com)
Human2
- There is limited information regarding targeting fatty acid synthesis in human pathogenic fungi. (immune-source.com)
- At Enzyvant Sciences, it is thought that the recombinant human acid ceramidase (rhAC) program will benefit from the continued involvement of the experienced medical and scientific team from Plexcera, while gaining the pharmaceutical development expertise and financial support of Roivant. (lysosomaldiseasenetwork.org)
Metabolic1
- High-fat feeding in time-dependent manner affects metabolic routes leading to nervonic acid synthesis in NAFLD. (edu.pl)
Compartments1
- At the electron microscope level, membrane-bound vacuoles or compartments were recognized and shown to contain acid hydrolases that were detectable by cytochemical staining (15). (schoolbag.info)
Cells2
- Gangliosides are series of glycosphingolipids containing sialic acids in the oligosaccharide portion in mammalian cells. (encyclopedia.pub)
- a lot more than 90% from the cells had been gathered in Azelaic acid the MB level when the proportion of the MBs to cells was greater than 70:1. (cell-signaling-pathways.com)
Serum2
- The association between serum uric acid (SUA) and the components of dyslipidaemia and their dose-response relationships have not been thoroughly explored. (biomedcentral.com)
- acid synthase dies after incubation in various media including serum. (immune-source.com)
Activation1
- Flufenamic acid-induced plasma membrane hyperpolarization and totally abolished the activation by STS from the depolarization conductance. (cell-signaling-pathways.com)