Anhydrides: Chemical compounds derived from acids by the elimination of a molecule of water.Phthalic Anhydrides: Phthalic acid anhydrides. Can be substituted on any carbon atom. Used extensively in industry and as a reagent in the acylation of amino- and hydroxyl groups.Maleic Anhydrides: Used in copolymerization reactions, in the Diels-Alder(diene)synthesis, in the preparation of resins, pharmaceuticals and agricultural chemicals. It is a powerful irritant and causes burns.Acid Anhydride Hydrolases: A group of enzymes that catalyze the hydrolysis of diphosphate bonds in compounds such as nucleoside di- and tri-phosphates, and sulfonyl-containing anhydrides such as adenylylsulfate. (Enzyme Nomenclature, 1992) EC 3.6.Succinic Anhydrides: A subclass of anhydrides with the general structure of dihydrofurandione. They can be substituted on any carbon atom. They modify and inhibit proteins and enzymes and are used in the acylation of amino- and hydroxyl groups.Acetic Anhydrides: Compounds used extensively as acetylation, oxidation and dehydrating agents and in the modification of proteins and enzymes.Aconitic AcidHydrolases: Any member of the class of enzymes that catalyze the cleavage of the substrate and the addition of water to the resulting molecules, e.g., ESTERASES, glycosidases (GLYCOSIDE HYDROLASES), lipases, NUCLEOTIDASES, peptidases (PEPTIDE HYDROLASES), and phosphatases (PHOSPHORIC MONOESTER HYDROLASES). EC 3.Respiration Disorders: Diseases of the respiratory system in general or unspecified or for a specific respiratory disease not available.Citraconic Anhydrides: Methylmaleic anhydrides.Occupational Exposure: The exposure to potentially harmful chemical, physical, or biological agents that occurs as a result of one's occupation.Occupational Diseases: Diseases caused by factors involved in one's employment.Glycoside Hydrolases
(1/700) A novel interaction mechanism accounting for different acylphosphatase effects on cardiac and fast twitch skeletal muscle sarcoplasmic reticulum calcium pumps.

In cardiac and skeletal muscle Ca2+ translocation from cytoplasm into sarcoplasmic reticulum (SR) is accomplished by different Ca2+-ATPases whose functioning involves the formation and decomposition of an acylphosphorylated phosphoenzyme intermediate (EP). In this study we found that acylphosphatase, an enzyme well represented in muscular tissues and which actively hydrolyzes EP, had different effects on heart (SERCA2a) and fast twitch skeletal muscle SR Ca2+-ATPase (SERCA1). With physiological acylphosphatase concentrations SERCA2a exhibited a parallel increase in the rates of both ATP hydrolysis and Ca2+ transport; in contrast, SERCA1 appeared to be uncoupled since the stimulation of ATP hydrolysis matched an inhibition of Ca2+ pump. These different effects probably depend on phospholamban, which is associated with SERCA2a but not SERCA1. Consistent with this view, the present study suggests that acylphosphatase-induced stimulation of SERCA2a, in addition to an enhanced EP hydrolysis, may be due to a displacement of phospholamban, thus to a removal of its inhibitory effect.  (+info)

(2/700) Characterization of the nucleoside triphosphatase activity of poliovirus protein 2C reveals a mechanism by which guanidine inhibits poliovirus replication.

The highly conserved non-structural protein 2C of picornaviruses is involved in viral genome replication and encapsidation and in the rearrangement of intracellular structures. 2C binds RNA, has nucleoside triphosphatase activity, and shares three motifs with superfamily III helicases. Motifs "A" and "B" are involved in nucleotide triphosphate (NTP) binding and hydrolysis, whereas a function for motif "C" has not yet been demonstrated. Poliovirus RNA replication is inhibited by millimolar concentrations of guanidine hydrochloride (GdnHCl). Resistance and dependence to GdnHCl map to 2C. To characterize the nucleoside triphosphatase activity of 2C, we purified poliovirus recombinant 2C fused to glutathione S-transferase (GST-2C) from Escherichia coli. GST-2C hydrolyzed ATP with a Km of 0.7 mM. Other NTPs, including GTP, competed with ATP for binding to 2C but were poor substrates for hydrolysis. Mutation of conserved residues in motif A and B abolished ATPase activity, as did mutation of the conserved asparagine residue in motif C, an observation indicating the involvement of this motif in ATP hydrolysis. GdnHCl at millimolar concentrations inhibited ATP hydrolysis. Mutations in 2C that confer poliovirus resistant to or dependent on GdnHCl increased the tolerance to GdnHCl up to 100-fold.  (+info)

(3/700) The serine protease and RNA-stimulated nucleoside triphosphatase and RNA helicase functional domains of dengue virus type 2 NS3 converge within a region of 20 amino acids.

NS3 protein of dengue virus type 2 has a serine protease domain within the N-terminal 180 residues. NS2B is required for NS3 to form an active protease involved in processing of the viral polyprotein precursor. The region carboxy terminal to the protease domain has conserved motifs present in several viral RNA-stimulated nucleoside triphosphatase (NTPase)/RNA helicases. To define the functional domains of protease and NTPase/RNA helicase activities of NS3, full-length and amino-terminal deletion mutants of NS3 were expressed in Escherichia coli and purified. Deletion of 160 N-terminal residues of NS3 (as in NS3del.2) had no detrimental effect on the basal and RNA-stimulated NTPase as well as RNA helicase activities. However, mutagenesis of the conserved P-loop motif of the RNA helicase domain (K199E) resulted in loss of ATPase activity. The RNA-stimulated NTPase activity was significantly affected by deletion of 20 amino acid residues from the N terminus or by substitutions of the cluster of basic residues, 184RKRK-->QNGN, of NS3del.2, although both mutant proteins retained the conserved RNA helicase motifs. Furthermore, the minimal NS3 protease domain, required for cleavage of the 2B-3 site, was precisely defined to be 167 residues, using the in vitro processing of NS2B-NS3 precursors. Our results reveal that the functional domains required for serine protease and RNA-stimulated NTPase activities map within the region between amino acid residues 160 and 180 of NS3 protein and that a novel motif, the cluster of basic residues 184RKRK, plays an important role for the RNA-stimulated NTPase activity.  (+info)

(4/700) The Saccharomyces cerevisiae YOR163w gene encodes a diadenosine 5', 5"'-P1,P6-hexaphosphate (Ap6A) hydrolase member of the MutT motif (Nudix hydrolase) family.

The YOR163w open reading frame on chromosome XV of the Saccharomyces cerevisiae genome encodes a member of the MutT motif (nudix hydrolase) family of enzymes of Mr 21,443. By cloning and expressing this gene in Escherichia coli and S. cerevisiae, we have shown the product to be a (di)adenosine polyphosphate hydrolase with a previously undescribed substrate specificity. Diadenosine 5',5"'-P1, P6-hexaphosphate is the preferred substrate, and hydrolysis in H218O shows that ADP and adenosine 5'-tetraphosphate are produced by attack at Pbeta and AMP and adenosine 5'-pentaphosphate are produced by attack at Palpha with a Km of 56 microM and kcat of 0.4 s-1. Diadenosine 5',5"'-P1,P5-pentaphosphate, adenosine 5'-pentaphosphate, and adenosine 5'-tetraphosphate are also substrates, but not diadenosine 5',5"'-P1,P4-tetraphosphate or other dinucleotides, mononucleotides, nucleotide sugars, or nucleotide alcohols. The enzyme, which was shown to be expressed in log phase yeast cells by immunoblotting, displays optimal activity at pH 6.9, 50 degrees C, and 4-10 mM Mg2+ (or 200 microM Mn2+). It has an absolute requirement for a reducing agent, such as dithiothreitol (1 mM), and is inhibited by Ca2+ with an IC50 of 3.3 mM and F- (noncompetitively) with a Ki of 80 microM. Its function may be to eliminate potentially toxic dinucleoside polyphosphates during sporulation.  (+info)

(5/700) Characterization and mutational analysis of the helicase and NTPase activities of hepatitis C virus full-length NS3 protein.

The non-structural protein 3 (NS3) of hepatitis C virus (HCV) possesses three activities which are likely to be essential for virus replication; a serine protease located in the N terminus and helicase and NTPase activities located in the C terminus. Sequence analysis of the helicase/NTPase domain has identified motifs indicative of the DEAD-box family of helicases. Here we present the characterization of the helicase and NTPase activities of full-length NS3, expressed as a His-tagged fusion protein in E. coli, and make comparisons with published data of NS3 helicase domain alone. The helicase and NTPase activities of full-length NS3 have been demonstrated and we have characterized the effects of amino acid substitutions on conserved motifs of NS3 helicase. Helicase and NTPase activities were dependent on Mg2+ and ATP and inhibited by monovalent cations. NS3 was able to hydrolyse all four NTPs and dNTPs to drive DNA duplex unwinding but with differing abilities. NTPase activity was stimulated by all polynucleotides tested, with poly(U) having the greatest effect. Mutational analysis of conserved motifs of NS3 helicase showed all conserved residues to be required for optimal activity. These results are in accord with a recently proposed model for NS3 helicase activity.  (+info)

(6/700) The FHIT gene is expressed in pancreatic ductular cells and is altered in pancreatic cancers.

We examined 2 normal pancreata, 21 primary pancreatic ductal cancers, and 19 pancreatic cancer cell lines for Fhit expression and FHIT gene status. The normal pancreas expressed Fhit protein in the cytoplasm of ductular cells, whereas interlobular and larger ducts, acini, and insulae of Langerhans were negative. Fhit protein was detected by immunoblot assay in 11 pancreatic cancer cell lines; of the 8 cell lines lacking Fhit protein, 7 lacked FHIT mRNA and 1 showed an abnormally sized transcript. DNA from five of these eight cell lines showed homozygous loss of FHIT exon 5. In 8 of the 21 primary cancers, Fhit expression was detected by immunohistochemistry. Reverse transcription-PCR analysis of 6 of the 13 cases lacking Fhit showed normal-sized FHIT product in 3 cases and a mixture of normal and abnormal products in the other 3. Sequencing showed that abnormal bands were missing variable numbers of exons. Loss of microsatellite DNA markers internal to the FHIT gene was observed in 10 of 13 primary cancers lacking Fhit protein (homozygous in two cases) and in only 1 of the 8 cancers expressing Fhit protein. In nine primary cancers, four expressing and five lacking Fhit protein, it was possible to obtain pure cancer DNA by microdissection. Three of the five microdissected cases lacking Fhit protein exhibited homozygous deletion of FHIT exon 5. In conclusion, the lack of Fhit protein in pancreatic cancers correlated with absence or alteration of FHIT mRNA and was often associated with FHIT gene anomalies.  (+info)

(7/700) Loss of heterozygosity at 3p14.2 in clear cell renal cell carcinoma is an early event and is highly localized to the FHIT gene locus.

The VHL tumor suppressor gene (TSG) at 3p25-26 is strongly implicated in the pathogenesis of clear cell renal cell carcinoma (cRCC). In addition, 3p14.2 and 3p21 are suspected of harboring additional TSGs in cRCC, with FHIT being a candidate TSG at 3p14.2. We examined 87 microdissected, histologically well-defined cRCCs classified according to tumor-node-metastasis (TNM) stage (stage 1, 23 cases; stage 2, 14 cases; stage 3, 24 cases; stage 4, 26 cases) and Fuhrman grade (grade 1, 24 cases; grade 2, 19 cases; grade 3, 19 cases; grade 4, 8 cases; sarcomatoid cRCC, 17 cases) for loss of heterozygosity (LOH) at 3p14.2 and 3p25-26 using a series of precisely mapped microsatellite probes. We found that LOH at 3p14.2 exceeded LOH at 3p25-26 in frequency (69% versus 48.3%; P < 0.03) and was highly localized to markers within the FHIT gene locus (D3S1300 and D3S4260), with the majority of chromosomal breakpoints also mapping to this region. In addition, 3p14.2 LOH (P < 0.03), but not 3p25-26 LOH (P = nonsignificant), was associated with lower tumor grades (grades 1-3). These findings suggest that 3p14.2 genomic deletions may be among the earliest events in cRCC pathogenesis, preceding genomic deletions at the VHL locus. FHIT, or an as yet undiscovered TSG mapping to the D3S4103-D3S4260 interval, could be the molecular target of the 3p14.2 deletions.  (+info)

(8/700) Designing conditions for in vitro formation of amyloid protofilaments and fibrils.

We have been able to convert a small alpha/beta protein, acylphosphatase, from its soluble and native form into insoluble amyloid fibrils of the type observed in a range of pathological conditions. This was achieved by allowing slow growth in a solution containing moderate concentrations of trifluoroethanol. When analyzed with electron microscopy, the protein aggregate present in the sample after long incubation times consisted of extended, unbranched filaments of 30-50 A in width that assemble subsequently into higher order structures. This fibrillar material possesses extensive beta-sheet structure as revealed by far-UV CD and IR spectroscopy. Furthermore, the fibrils exhibit Congo red birefringence, increased fluorescence with thioflavine T and cause a red-shift of the Congo red absorption spectrum. All of these characteristics are typical of amyloid fibrils. The results indicate that formation of amyloid occurs when the native fold of a protein is destabilized under conditions in which noncovalent interactions, and in particular hydrogen bonding, within the polypeptide chain remain favorable. We suggest that amyloid formation is not restricted to a small number of protein sequences but is a property common to many, if not all, natural polypeptide chains under appropriate conditions.  (+info)

*  Acid anhydride hydrolases
... are a class of hydrolase enzymes that catalyze the hydrolysis of an acid anhydride bond. They are ... 2012). Acid Anhydride Hydrolases: Advances in Research and Application. Acid anhydride hydrolases at the US National Library of ... List of EC numbers (EC 3)#EC 3.6: act on acid anhydrides Ashton Acton, ed. ( ...
*  Pyrophosphatase
... s are acid anhydride hydrolases that act upon diphosphate bonds. Examples include: Inorganic pyrophosphatase ...
*  Thiamine-triphosphatase
... phosphate This enzyme belongs to the family of acid anhydride hydrolases, specifically those acting on phosphorus-containing ... anhydrides. Its systematic name is thiamine triphosphate phosphohydrolase. As of late 2007, only one structure has been solved ...
*  Hydrolase
... other than peptide bonds EC 3.6 acid anhydrides (acid anhydride hydrolases, including helicases and GTPase) EC 3.7 carbon- ... a nuclease is a hydrolase that cleaves nucleic acids. Hydrolases are classified as EC 3 in the EC number classification of ... Systematic names of hydrolases are formed as "substrate hydrolase." However, common names are typically in the form " ... In biochemistry, a hydrolase is an enzyme that catalyzes the hydrolysis of a chemical bond. For example, an enzyme that ...
*  Phosphoribosyl-ATP diphosphatase
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing ... anhydrides. The systematic name of this enzyme class is 1-(5-phosphoribosyl)-ATP diphosphohydrolase. Other names in common use ...
*  Triphosphatase
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing ... anhydrides. The systematic name of this enzyme class is triphosphate phosphohydrolase. This enzyme is also called inorganic ...
*  Acylphosphatase
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing ... anhydrides. The systematic name of this enzyme class is acylphosphate phosphohydrolase. Other names in common use include ...
*  Diphosphoinositol-polyphosphate diphosphatase
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing ... anhydrides. The systematic name of this enzyme class is diphospho-myo-inositol-polyphosphate diphosphohydrolase. Other names in ...
*  Trimetaphosphatase
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing ... anhydrides. The systematic name of this enzyme class is trimetaphosphate hydrolase. This enzyme is also called inorganic ...
*  Adenylylsulfatase
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in sulfonyl-containing anhydrides ...
*  Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical)
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing ... anhydrides. The systematic name of this enzyme class is P1,P4-bis(5'-nucleosyl)-tetraphosphate nucleotidohydrolase. Other names ...
*  Nucleoside-triphosphate diphosphatase
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing ... anhydrides. The systematic name of this enzyme class is nucleoside-triphosphate diphosphohydrolase. This enzyme is also called ...
*  CDP-glycerol diphosphatase
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing ... GLASER L (1965). "THE SYNTHESIS OF TEICHOIC ACID. IV. ON THE REGULATION OF CYTIDINE 5'-DIPHOSPHATEGLYCEROL CONCENTRATION". ... anhydrides. The systematic name of this enzyme class is CDP-glycerol phosphoglycerohydrolase. Other names in common use include ...
*  ADP-sugar diphosphatase
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing ... anhydrides. The systematic name of this enzyme class is ADP-sugar sugarphosphohydrolase. Other names in common use include ADP- ...
*  Thymidine-triphosphatase
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing ... anhydrides. The systematic name of this enzyme class is dTTP nucleotidohydrolase. Other names in common use include thymidine ...
*  Guanosine-5'-triphosphate,3'-diphosphate diphosphatase
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing ... anhydrides. The systematic name of this enzyme class is guanosine-5'-triphosphate,3'-diphosphate 5'-phosphohydrolase. Other ...
*  Inorganic diphosphatase
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing ... anhydrides. The systematic name of this enzyme class is diphosphate phosphohydrolase. This enzyme participates in oxidative ...
*  Bis(5'-adenosyl)-triphosphatase
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing ... anhydrides. The systematic name of this enzyme class is P1,P3-bis(5'-adenosyl)-triphosphate adenylohydrolase. Other names in ...
*  Adenosine-tetraphosphatase
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing ... Small GD, Cooper C (1966). "Purification and properties of nucleoside tetraphosphate hydrolase from rabbit muscle". ... anhydrides. The systematic name of this enzyme class is adenosine-tetraphosphate phosphohydrolase. This enzyme participates in ...
*  M7G(5')pppN diphosphatase
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing ... anhydrides. The systematic name of this enzyme class is 7-methylguanosine-5'-triphospho-5'-polynucleotide 7-methylguanosine-5'- ...
*  Nucleoside-triphosphatase
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing ... Brightwell R, Tappel AL (1968). "Lysosomal acid pyrophosphatase and acid phosphatase". Arch. Biochem. Biophys. 124 (1): 333-43 ... anhydrides. The systematic name of this enzyme class is unspecific diphosphate phosphohydrolase. Other names in common use ...
*  Guanosine-diphosphatase
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing ... anhydrides. The systematic name of this enzyme class is GDP phosphohydrolase. This enzyme is also called GDPase. Raychaudhuri P ...
*  Oligosaccharide-diphosphodolichol diphosphatase
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing ... anhydrides. The systematic name of this enzyme class is oligosaccharide-diphosphodolichol phosphodolichohydrolase. This enzyme ...
*  UDP-sugar diphosphatase
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing ... Glaser L, Melo A, Paul R (1987). "Uridine diphosphate sugar hydrolase. Purification of enzyme and protein inhibitor". J. Biol. ... UDP-sugar hydrolase, and UDP-sugar pyrophosphatase. As of late 2007, 6 structures have been solved for this class of enzymes, ... anhydrides. The systematic name of this enzyme class is UDP-sugar sugarphosphohydrolase. Other names in common use include ...
*  Bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides in phosphorus-containing ... Ap4A hydrolase, bis(5'-adenosyl) tetraphosphatase, diadenosine tetraphosphate hydrolase, diadenosine polyphosphate hydrolase, ... anhydrides. The systematic name of this enzyme class is P1,P4-bis(5'-nucleosyl)-tetraphosphate nucleosidebisphosphohydrolase. ... symmetrical diadenosine tetraphosphate hydrolase, adenosine tetraphosphate phosphodiesterase, ...
*  Phosphate-transporting ATPase
This enzyme belongs to the family of hydrolases, specifically those acting on acid anhydrides to catalyse transmembrane ...
Acid anhydride hydrolases - Wikipedia  Acid anhydride hydrolases - Wikipedia
Acid anhydride hydrolases are a class of hydrolase enzymes that catalyze the hydrolysis of an acid anhydride bond. They are ... 2012). Acid Anhydride Hydrolases: Advances in Research and Application. Acid anhydride hydrolases at the US National Library of ... List of EC numbers (EC 3)#EC 3.6: act on acid anhydrides Ashton Acton, ed. ( ...
more infohttps://en.wikipedia.org/wiki/Acid_anhydride_hydrolases
Pyrophosphatase - Wikipedia  Pyrophosphatase - Wikipedia
Pyrophosphatases are acid anhydride hydrolases that act upon diphosphate bonds. Examples include: Inorganic pyrophosphatase ...
more infohttps://en.wikipedia.org/wiki/Pyrophosphatase
bis(5-nucleosyl)tetraphosphatase (asymmetrical)
     Summary Report | CureHunter  bis(5'-nucleosyl)tetraphosphatase (asymmetrical) Summary Report | CureHunter
Hydrolases: 639*Acid Anhydride Hydrolases*bis(5'-nucleosyl)tetraphosphatase (asymmetrical): 3. Related Diseases. 1. Shock 07/01 ... AP4A hydrolase; Ap4A phosphodiesterase (unsymmetrical); bis(5'-adenosyl)tetraphosphatase; bis(5'-guanosyl) tetraphosphatase; ... "By investigating the intracellular level of Ap4A hydrolase under different kinds of environmental stress, no changes occurred ...
more infohttp://www.curehunter.com/public/keywordSummaryC020023-bis-5--nucleosyl-tetraphosphatase--asymmetrical-.do
rho GTP-Binding Proteins | Harvard Catalyst Profiles | Harvard Catalyst  rho GTP-Binding Proteins | Harvard Catalyst Profiles | Harvard Catalyst
Acid Anhydride Hydrolases [D08.811.277.040]. *GTP Phosphohydrolases [D08.811.277.040.330]. *GTP-Binding Proteins [D08.811. ... Masago K, Kihara Y, Yanagida K, Hamano F, Nakagawa S, Niwa M, Shimizu T. Lysophosphatidic acid receptor, LPA6, regulates ...
more infohttps://connects.catalyst.harvard.edu/Profiles/display/Concept/rho%20GTP-Binding%20Proteins
Polyamine metabolism and related enzymes. Shadows indic | Open-i  Polyamine metabolism and related enzymes. Shadows indic | Open-i
Acid Anhydride Hydrolases/genetics. *Base Sequence. *Molecular Sequence Data. *Phosphate Transport Proteins/genetics ... In addition, similarities of M. phosphovorus speA to non-actinobacterial genes are modest, with amino acid identities less than ... In addition, similarities of M. phosphovorus speA to non-actinobacterial genes are modest, with amino acid identities less than ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC3473371_dss02003&req=4
Molecular phylogenetic analyses of the (A) polyP kinase | Open-i  Molecular phylogenetic analyses of the (A) polyP kinase | Open-i
Acid Anhydride Hydrolases/genetics. *Base Sequence. *Molecular Sequence Data. *Phosphate Transport Proteins/genetics ... Amino acid sequences deduced from nucleotide sequences were used as operational taxonomic units (OTUs) for the analyses. All ... Amino acid sequences deduced from nucleotide sequences were used as operational taxonomic units (OTUs) for the analyses. All ... Based on the similarity of deduced amino acid sequences, one of the M. phosphovorus PPK2s (MLP_05750) was of the C. glutamicum ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC3473371_dss02002&req=4
Guanosine Triphosphate Phosphohydrolases - Semantic Scholar  Guanosine Triphosphate Phosphohydrolases - Semantic Scholar
This is a class of proteins that belongs to a family of high energy phosphate hydrolases. Members of this family play major ... Acid Anhydride Hydrolases. DNM1L protein, human. DNM2 protein, human. EEF1A1 protein, human. EEF1A2 protein, human ... The mTORC1 kinase promotes growth in response to growth factors, energy levels, and amino acids, and its activity is often… ( ... Ragulator-Rag Complex Targets mTORC1 to the Lysosomal Surface and Is Necessary for Its Activation by Amino Acids ...
more infohttps://www.semanticscholar.org/topic/Guanosine-Triphosphate-Phosphohydrolases/3660
thapsigargin (CHEBI:9516)  thapsigargin (CHEBI:9516)
An EC 3.6.3.* (acid anhydride hydrolase catalysing transmembrane movement of substances) inhibitor that interferes with the ... octanoic acid {3S-. [3α,3aβ,4α,6β,6aβ,7β,8α(Z),9bα]}-. 6-. (acetoxy)-. 2,3,3a,4,5,6,6a,7,8,9b-. decahydro-. 3,3a-. dihydroxy- ...
more infohttps://www.ebi.ac.uk/chebi/searchId.do?chebiId=9516
G protein  G protein
Acid anhydride hydrolases: GTPases/G proteins (EC 3.6.5). 3.6.5.1: Heterotrimeric G protein. Gαs - Gαi - Gαq/11 - Gα12/13 - ...
more infohttp://www.chemeurope.com/en/encyclopedia/G_protein.html
A nuclear protein in Schizosaccharomyces pombe with homology to the human tumour suppressor Fhit has decapping activity. - The...  A nuclear protein in Schizosaccharomyces pombe with homology to the human tumour suppressor Fhit has decapping activity. - The...
Acid Anhydride Hydrolases. -. dc.subject.mesh. Amino Acid Sequence. -. dc.subject.mesh. Gene Expression Regulation, Fungal. - ... Sequence Homology, Amino Acid. -. dc.title. A nuclear protein in Schizosaccharomyces pombe with homology to the human tumour ... Cloning of the Schizosaccharomyces pombe gene encoding diadenosine 5',5"'-P1,P4-tetraphosphate (Ap4A) asymmetrical hydrolase: ... P4-tetraphosphate asymmetrical hydrolase of S. pombe. Our experimental work also reveals that Nhm1 inhibits translation in a ...
more infohttp://christie.openrepository.com/christie/handle/10541/84341
GTP-Binding Proteins | Profiles RNS  GTP-Binding Proteins | Profiles RNS
Acid Anhydride Hydrolases [D08.811.277.040]. *GTP Phosphohydrolases [D08.811.277.040.330]. *GTP-Binding Proteins [D08.811. ... Topical delivery of 13-cis-retinoic acid by inhalation up-regulates expression of rodent lung but not liver retinoic acid ...
more infohttps://profiles.rush.edu/display/11121
Minichromosome Maintenance Proteins | Colorado PROFILES  Minichromosome Maintenance Proteins | Colorado PROFILES
Acid Anhydride Hydrolases [D08.811.277.040]. *Adenosine Triphosphatases [D08.811.277.040.025]. *DNA Helicases [D08.811.277.040. ...
more infohttps://profiles.ucdenver.edu/display/21256616
Vacuolar Proton-Translocating ATPases | REACH  Vacuolar Proton-Translocating ATPases | REACH
Acid Anhydride Hydrolases [D08.811.277.040]. *Adenosine Triphosphatases [D08.811.277.040.025]. *Proton-Translocating ATPases [ ...
more infohttps://reach.sickkids.ca/display/11775
rab3 GTP-Binding Proteins | Colorado PROFILES  rab3 GTP-Binding Proteins | Colorado PROFILES
Acid Anhydride Hydrolases [D08.811.277.040]. *GTP Phosphohydrolases [D08.811.277.040.330]. *GTP-Binding Proteins [D08.811. ...
more infohttps://profiles.ucdenver.edu/display/199561
Hydrolases [EC 3] (Hydrolase Enzymes)  < Enzymes  << Biocatalysts  <<< Catalysts  @...  Hydrolases [EC 3] (Hydrolase Enzymes) < Enzymes << Biocatalysts <<< Catalysts @...
Hydrolase Enzyme) is a class of enzymes that catalyze the cleavage of the substrate and the addition of water to the resulting ... "Acid Anhydride Hydrolases [EC 3.6]" Acid Anhydrides. In enzyme science, Acid Anhydride Hydrolases are a class of hydrolase ... "Hydrolases (Hydrolase Enzymes)" [EC 3] Hydrolase Reactions. In enzyme science, Hydrolases (Hydrolase Enzymes), as hydrolysis ... The peptide hydrolase enzymes include: *aspartic acid proteases (aspartic acid proteinases) *aspartic acid endopeptidases * ...
more infohttp://wellnessadvocate.com/?uid=60103
Hydrolases (Hydrolase Enzymes)  < Enzyme Reactions  << Enzymes  <<< Biocatalysts  @...  Hydrolases (Hydrolase Enzymes) < Enzyme Reactions << Enzymes <<< Biocatalysts @...
Hydrolase Enzyme) is a class of enzymes that catalyze the cleavage of the substrate and the addition of water to the resulting ... Acid Anhydride Hydrolases Acid Anhydrides. In enzyme science, Acid Anhydride Hydrolases are a class of hydrolase enzyme ... Hydrolases (Hydrolase Enzymes) Hydrolases (Hydrolase Enzymes) [EC 3] Hydrolase Reactions. In enzyme science, Hydrolases ( ... The peptide hydrolases includes: *aspartic acid proteases (aspartic acid proteinases) *aspartic acid endopeptidases *Cathepsin ...
more infohttp://wellnessadvocate.com/?dgl=2283
Expression of biologically active human <i>butyrylcholinesterase</i>  by Peter L. Platteborze and Clarence...  "Expression of biologically active human <i>butyrylcholinesterase</i> " by Peter L. Platteborze and Clarence...
This tolerance may be attributed at least in part to a novel endogenous organophosphorus acid anhydride hydrolase activity that ... This tolerance may be attributed at least in part to a novel endogenous organophosphorus acid anhydride hydrolase activity that ...
more infohttp://digitalcommons.unl.edu/usarmyresearch/311/
RCSB PDB - 1NGB: STRUCTURAL BASIS OF THE 70-KILODALTON HEAT SHOCK COGNATE PROTEIN ATP HYDROLYTIC ACTIVITY, II. STRUCTURE OF THE...  RCSB PDB - 1NGB: STRUCTURAL BASIS OF THE 70-KILODALTON HEAT SHOCK COGNATE PROTEIN ATP HYDROLYTIC ACTIVITY, II. STRUCTURE OF THE...
Classification: HYDROLASE(ACTING ON ACID ANHYDRIDES). *Organism(s): Bos taurus. *Deposited: 1994-05-17 Released: 1994-08-31 ...
more infohttp://www.rcsb.org/structure/1NGB
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Acid Anhydride Hydrolases, antagonists & inhibitors, metabolism, Alkaline Phosphatase, Animals, Cations, Divalent, Cattle, ... Amino Acid Sequence, Amino Acids, analysis, Animals, Antifreeze Proteins, Type I, chemistry, isolation & purification, Atlantic ... Amino Acid Sequence, Enzyme Inhibitors, pharmacology, Mass Spectrometry, methods, Models, Chemical, Molecular Sequence Data, ... Amino Acid Substitution, Catalysis, Enzyme Activation, Enzyme Stability, Kinetics, Mutagenesis, Site-Directed, Mutation, ...
more infohttps://academic.naver.com/search.naver?field=3&query=Biochimica+et+Biophysica+Acta+1547%EA%B6%8C+2%ED%98%B8
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Acid Anhydride Hydrolases, biosynthesis, genetics, Animals, Body Weight, drug effects, Carcinogens, toxicity, Gene Expression ... The present study was carried out to study the effect of gentisic acid (2,5-dihydroxybenzoic acid (2,5-DHBA)) on the tumor ... Modulatory effect of gentisic acid on the augmentation of biochemical events of tumor promotion stage by benzoyl peroxide and ...
more infohttp://academic.naver.com/search.naver?field=3&query=Toxicology+Letters+153%EA%B6%8C+3%ED%98%B8
  • Two hydrolase resistant analogues of diadenosine 5',5"'-P1,P3-triphosphate for studies with Fhit, the human fragile histidine triad protein. (nih.gov)
  • However, the assumption that Fhit operates by water attack at P 1 is insecure, not least because the lack of sequence homology between lupin and human Ap4A hydrolases and the Ap3A hydrolase from S. pombe makes it imperative to generate analogues of Ap3A that are able to resist water attack at either P 1 (P 3 ) or P 2 . (nih.gov)
  • Effect of different nucleic acid oligonucleotides on ATPase activity: CHIKV-nsP2T protein was incubated in a 50 µl reaction containing 50 mM MOPS at pH 7.25, 1 mM ATP, 1 mM MgCl2, and different homopolynucleotides (25 ng/µl of the reaction), at 37°C for 30 min. (nih.gov)
  • GO annotations related to this gene include nucleic acid binding and helicase activity . (genecards.org)
  • Topical delivery of 13-cis-retinoic acid by inhalation up-regulates expression of rodent lung but not liver retinoic acid receptors. (rush.edu)
  • With extra background reading, you might find out that retinoic acid receptors can function as transcriptional regulators, but there is nothing in the term name or definition to suggest any relationship with transcriptional regulators. (geneontology.org)
  • In addition, similarities of M. phosphovorus speA to non-actinobacterial genes are modest, with amino acid identities less than 33%, obscuring the exact origin of this gene. (nih.gov)
  • Amino acid sequences deduced from nucleotide sequences were used as operational taxonomic units (OTUs) for the analyses. (nih.gov)
  • Based on the similarity of deduced amino acid sequences, one of the M. phosphovorus PPK2s (MLP_05750) was of the C. glutamicum type (63% identity), whereas the other (MLP_50300) was of the Myc. (nih.gov)
  • The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. (semanticscholar.org)
  • NTPase activity of helicase-like protein is typically stimulated by nucleic acids, particularly by poly (U). To test that, different concentrations of Poly (U) (125 ng to 5000 ng/reaction) were added and released phosphate was measured. (nih.gov)
  • Its sequence domains include a P-loop containing nucleoside triphosphate hydrolase and a small GTP-binding protein domain. (wikipedia.org)
  • This tolerance may be attributed at least in part to a novel endogenous organophosphorus acid anhydride hydrolase activity that is capable of hydrolysing the chemical-warfare nerve agents sarin (isopropyl methylphosphonofluoridate) and soman (pinacolyl methylphosphonofluoridate). (unl.edu)
  • A good example is the term retinoic acid receptor activity, which was wrongly given the parents receptor activity and transcription regulator activity. (geneontology.org)
  • The gene product retinoic acid receptor alpha could be annotated to retinoic acid receptor activity, and, by reasoning over the GO graph, it would also be annotated to the parent terms receptor activity and transcription regulator activity. (geneontology.org)
  • The correct way to deal with this situation is to remove the link in the ontology between retinoic acid receptor activity and transcription regulator activity and capture the information in the annotation instead. (geneontology.org)
  • We would annotate retinoic acid receptor alpha to both retinoic acid receptor activity and transcription regulator activity. (geneontology.org)
  • The present study was carried out to study the effect of gentisic acid (2,5-dihydroxybenzoic acid (2,5-DHBA)) on the tumor promotion related events of carcinogenesis in. (naver.com)