Achromobacter cycloclastes: A species of gram negative, aerobic, denitrifying bacteria in the genus ACHROMOBACTER.Alcaligenes: A genus of gram-negative, aerobic, motile bacteria that occur in water and soil. Some are common inhabitants of the intestinal tract of vertebrates. These bacteria occasionally cause opportunistic infections in humans.Achromobacter: A genus of gram-negative, strictly aerobic, non-spore forming rods. Soil and water are regarded as the natural habitat. They are sometimes isolated from a hospital environment and humans.Nitrite Reductases: A group of enzymes that oxidize diverse nitrogenous substances to yield nitrite. (Enzyme Nomenclature, 1992) EC 1.Achromobacter denitrificans: The type species of gram negative, aerobic bacteria in the genus ACHROMOBACTER. Previously in the genus ALCALIGENES, the classification and nomenclature of this species has been frequently emended. The two subspecies, Achromobacter xylosoxidans subsp. denitrificans and Achromobacter xylosoxidans subsp. xylosoxidans are associated with infections.Azurin: A bacterial protein from Pseudomonas, Bordetella, or Alcaligenes which operates as an electron transfer unit associated with the cytochrome chain. The protein has a molecular weight of approximately 16,000, contains a single copper atom, is intensively blue, and has a fluorescence emission band centered at 308nm.Copper: A heavy metal trace element with the atomic symbol Cu, atomic number 29, and atomic weight 63.55.Gram-Negative Bacterial Infections: Infections caused by bacteria that show up as pink (negative) when treated by the gram-staining method.Alcaligenes faecalis: The type species of gram negative bacteria in the genus ALCALIGENES, found in soil. It is non-pathogenic, non-pigmented, and used for the production of amino acids.Moraxella: A genus of gram-negative, aerobic bacteria occurring as rods (subgenus Moraxella) or cocci (subgenus Branhamella). Its organisms are parasitic on the mucous membranes of humans and other warm-blooded animals.Delftia: A genus of gram-negative, strictly aerobic chemoorganotrophic bacteria, in the family COMAMONADACEAE.Carbofuran: A cholinesterase inhibitor that is used as a systemic insecticide, an acaricide, and nematocide. (From Merck Index, 11th ed)
(1/2) Atomic resolution structures of resting-state, substrate- and product-complexed Cu-nitrite reductase provide insight into catalytic mechanism.

Copper-containing nitrite reductases catalyze the reduction of nitrite to nitric oxide (NO), a key step in denitrification that results in the loss of terrestrial nitrogen to the atmosphere. They are found in a wide variety of denitrifying bacteria and fungi of different physiology from a range of soil and aquatic ecosystems. Structural analysis of potential intermediates in the catalytic cycle is an important goal in understanding enzyme mechanism. Using "crystal harvesting" and substrate-soaking techniques, we have determined atomic resolution structures of four forms of the green Cu-nitrite reductase, from the soil bacterium Achromobacter cycloclastes. These structures are the resting state of the enzyme at 0.9 A, two species exhibiting different conformations of nitrite bound at the catalytic type 2 Cu, one of which is stable and also has NO present, at 1.10 A and 1.15 A, and a stable form with the product NO bound side-on to the catalytic type 2 Cu, at 1.12 A resolution. These structures provide incisive insights into the initial binding of substrate, its repositioning before catalysis, bond breakage (O-NO), and the formation of a stable NO adduct.  (+info)

(2/2) Assessing the impact of denitrifier-produced nitric oxide on other bacteria.

A series of experiments was undertaken to learn more about the impact on other bacteria of nitric oxide (NO) produced during denitrification. The denitrifier Rhodobacter sphaeroides 2.4.3 was chosen as a denitrifier for these experiments. To learn more about NO production by this bacterium, NO levels during denitrification were measured by using differential mass spectrometry. This revealed that NO levels produced during nitrate respiration by this bacterium were in the low muM range. This concentration of NO is higher than that previously measured in denitrifiers, including Achromobacter cycloclastes and Paracoccus denitrificans. Therefore, both 2.4.3 and A. cycloclastes were used in this work to compare the effects of various NO levels on nondenitrifying bacteria. By use of bacterial overlays, it was found that the NO generated by A. cycloclastes and 2.4.3 cells during denitrification inhibited the growth of both Bacillus subtilis and R. sphaeroides 2.4.1 but that R. sphaeroides 2.4.3 caused larger zones of inhibition in the overlays than A. cycloclastes. Both R. sphaeroides 2.4.3 and A. cycloclastes induced the expression of the NO stress response gene hmp in B. subtilis. Taken together, these results indicate that there is variability in the NO concentrations produced by denitrifiers, but, irrespective of the NO levels produced, microbes in the surrounding environment were responsive to the NO produced during denitrification.  (+info)

*  Achromobacter cycloclastes
... is a Gram-negative, aerobic bacterium from the genus Achromobacter. The complete genome of A. ... cycloclastes has been sequenced. List of sequenced bacterial genomes Uniprot straininfo of Achromobacter cycloclastes NCBI ...
*  Nitrite reductase (NO-forming)
"The 2.3 angstrom X-ray structure of nitrite reductase from Achromobacter cycloclastes". Science. 253 (5018): 438-442. doi: ...
*  List of MeSH codes (B03)
Achromobacter MeSH B03.660.075.027.030.100 --- Achromobacter cycloclastes MeSH B03.660.075.027.030.120 --- Achromobacter ... Achromobacter cycloclastes MeSH B03.440.400.425.117.024.120 --- Achromobacter denitrificans MeSH B03.440.400.425.117.050 --- ...
RCSB PDB 









- 1NIC: THE STRUCTURE OF CU-NITRITE REDUCTASE FROM ACHROMOBACTER CYCLOCLASTES AT FIVE PH VALUES, WITH...  RCSB PDB - 1NIC: THE STRUCTURE OF CU-NITRITE REDUCTASE FROM ACHROMOBACTER CYCLOCLASTES AT FIVE PH VALUES, WITH...
The structure of copper-nitrite reductase from Achromobacter cycloclastes at five pH values, with NO2- bound and with type II ... Achromobacter cycloclastes EC#: 1.7.2.1 IUBMB Details: PH 6.0 ("KP") Gene Name(s): nirK ... THE STRUCTURE OF CU-NITRITE REDUCTASE FROM ACHROMOBACTER CYCLOCLASTES AT FIVE PH VALUES, WITH NITRITE BOUND AND WITH TYPE II CU ...
more infohttp://www.rcsb.org/pdb/explore/litView.do?structureId=1NIC
RCSB PDB 









- 1NIE: THE STRUCTURE OF CU-NITRITE REDUCTASE FROM ACHROMOBACTER CYCLOCLASTES AT FIVE PH VALUES, WITH...  RCSB PDB - 1NIE: THE STRUCTURE OF CU-NITRITE REDUCTASE FROM ACHROMOBACTER CYCLOCLASTES AT FIVE PH VALUES, WITH...
The structure of copper-nitrite reductase from Achromobacter cycloclastes at five pH values, with NO2- bound and with type II ... Achromobacter cycloclastes EC#: 1.7.2.1 IUBMB Details: PH 5.0 ("PH5") Gene Name(s): nirK ... THE STRUCTURE OF CU-NITRITE REDUCTASE FROM ACHROMOBACTER CYCLOCLASTES AT FIVE PH VALUES, WITH NITRITE BOUND AND WITH TYPE II CU ...
more infohttp://www.rcsb.org/pdb/explore/litView.do?structureId=1NIE
Genomic analysis reveals widespread occurrence of new classes of copper nitrite reductases | SpringerLink  Genomic analysis reveals widespread occurrence of new classes of copper nitrite reductases | SpringerLink
Achromobacter cycloclastes and Alcaligenes faecalis) NiRs which have been extensively studied and may indicate that these also ...
more infohttps://link.springer.com/article/10.1007%2Fs00775-007-0282-2
Patent US8017749 - Compositions and methods to treat cancer with cupredoxins and CpG rich DNA - Google Patents  Patent US8017749 - Compositions and methods to treat cancer with cupredoxins and CpG rich DNA - Google Patents
SEQ ID NO: 5. Amino acid sequence of pseudoazurin from Achromobacter cycloclastes. ... SEQ ID NO: 7. Amino acid sequence of azurin from Achromobacter xylosoxidans ssp. denitrificans I. ... In particularly specific embodiments, the azurin is derived from Pseudomonas aeruginosa, Alcaligenes faecalis, Achromobacter ... In particularly specific embodiments, the azurin is derived from Pseudomonas aeruginosa, Alcaligenes faecalis, Achromobacter ...
more infohttp://www.google.com/patents/US8017749?dq=5311516
Achromobacter cycloclastes - Wikipedia  Achromobacter cycloclastes - Wikipedia
Achromobacter cycloclastes is a Gram-negative, aerobic bacterium from the genus Achromobacter. The complete genome of A. ... cycloclastes has been sequenced. List of sequenced bacterial genomes Uniprot straininfo of Achromobacter cycloclastes NCBI ...
more infohttps://en.wikipedia.org/wiki/Achromobacter_cycloclastes
CCRC 16036 Strain Passport - StrainInfo  CCRC 16036 Strain Passport - StrainInfo
Achromobacter cycloclastes copper dependent nitrite reductase (nir) gene,partial cds. ATCC 21921 ... Achromobacter cycloclastes regulatory protein NosR (nosR), nitrous oxidereductase precursor (nosZ), accessory protein NosD ...
more infohttp://www.straininfo.net/strains/514100
Patent US4600692 - Immobilized cells for preparing phenylalanine - Google Patents  Patent US4600692 - Immobilized cells for preparing phenylalanine - Google Patents
Achromobacter cycloclastes. Sarcina lutea. Kluyvera citrophila. Pseudomonas fluorescens. Micrococcus lysodeikticus.. The ...
more infohttp://www.google.ca/patents/US4600692
PDB 2bwd structure summary ‹ Protein Data Bank in Europe (PDBe) ‹ EMBL-EBI  PDB 2bwd structure summary ‹ Protein Data Bank in Europe (PDBe) ‹ EMBL-EBI
Source organism: Achromobacter cycloclastes. Primary publication:. Atomic resolution structures of resting-state, substrate- ... Source organism: Achromobacter cycloclastes. UniProt: *Canonical: P25006 (Residues: 39-378; Coverage: 100%) ... Atomic Resolution Structure of Achromobacter cycloclastes Cu Nitrite Reductase with Endogenously bound Nitrite and NO. ...
more infohttp://www.ebi.ac.uk/pdbe/entry/pdb/2BWD
IUCr) IUCrJ Volume 4, Part 4, July 2017  IUCr) IUCrJ Volume 4, Part 4, July 2017
PDB references: Achromobacter cycloclastes nitrite reductase, data set ds1240K, 5n8f; data set ds2240K, 5n8g; data set ds3240K ... Active-site protein dynamics and solvent accessibility in native Achromobacter cycloclastes copper nitrite reductase. ...
more infohttp://journals.iucr.org/m/issues/2017/04/00/
KAKEN - Research Projects | 生体関連金属錯体における多重相互作用と機能  KAKEN - Research Projects | 生体関連金属錯体における多重相互作用と機能
Publications] T.Inoue: 'Crystallization and Preliminary X-ray Studies on Pseudoazurin from Achromobacter cycloclastes IAM 1013 ... Publications] T.Kohzuma: 'Direct Electrochemistry of Nitrite Reductase from Achromobacter cycloclastes IAM 1013.' Bull.Chem.Soc ... Publications] T.Kohzuma: 'Direct Electrochemistry of Copper-Containing Nitrite Reductase from Achromobacter xylosoxidans NCIB ...
more infohttps://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04225102/
SCOPe 2.07: Structural Classification of Proteins - extended  SCOPe 2.07: Structural Classification of Proteins - extended
Species: Achromobacter cycloclastes [TaxId:223]. Gene: nirK. Database cross-references and differences (RAF-indexed): *Uniprot ...
more infohttps://scop.berkeley.edu/pdb/code=5of6
Pseudoazurin (IPR012745) | InterPro | EMBL-EBI  Pseudoazurin (IPR012745) | InterPro | EMBL-EBI
Crystallization and preliminary X-ray studies on pseudoazurin from Achromobacter cycloclastes IAM1013.. J. Biochem. 114 761-2 ... Crystal structure determinations of oxidized and reduced pseudoazurins from Achromobacter cycloclastes. Concerted movement of ...
more infohttp://www.ebi.ac.uk/interpro/entry/IPR012745
Nitrite reductase (NO-forming) - Wikipedia  Nitrite reductase (NO-forming) - Wikipedia
"The 2.3 angstrom X-ray structure of nitrite reductase from Achromobacter cycloclastes". Science. 253 (5018): 438-442. doi: ...
more infohttps://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming)
Air-Sea Interactions of Natural Long-Lived Greenhouse Gases (CO2, N2O, CH4) in a Changing Climate | SpringerLink  Air-Sea Interactions of Natural Long-Lived Greenhouse Gases (CO2, N2O, CH4) in a Changing Climate | SpringerLink
... rebound mechanism for production of N2O from nitrite by the copper-containing nitrite reductase from Achromobacter-Cycloclastes ...
more infohttps://link.springer.com/chapter/10.1007/978-3-642-25643-1_3
Development of PCR Primer Systems for Amplification of Nitrite Reductase Genes (nirK and nirS) To Detect Denitrifying Bacteria...  Development of PCR Primer Systems for Amplification of Nitrite Reductase Genes (nirK and nirS) To Detect Denitrifying Bacteria...
Achromobacter cycloclastes (Z48635), Pseudomonas aureofaciens(Z21945), Pseudomonas sp. strain G-179 (M97294),Rhodobacter ... of uncharacterized dNir type were screened with this combination and an antiserum against Cu dNir from Alcaligenes cycloclastes ...
more infohttps://aem.asm.org/content/64/10/3769?ijkey=210b87ed1c1a552e05bf47a97c872ac3408f6331&keytype2=tf_ipsecsha
EC 1.7 and EC 1.8  EC 1.7 and EC 1.8
The 2.3 angstrom X-ray structure of nitrite reductase from Achromobacter cycloclastes. Science 253 (1991) 438-442. [PMID: ... 3. Prakash, O.M. and Sadana, J.C. Purification, characterization and properties of nitrite reductase of Achromobacter fischeri ...
more infohttps://www.qmul.ac.uk/sbcs/iubmb/enzyme/EC1/07p.html
Recently added  Recently added
Crystal structure refinement of pseudoazurin from Achromobacter cycloclastes (AcPAz) was carried out at atomic (1.10 angstrom) ...
more infohttps://scholarworks.montana.edu/xmlui/handle/1/8714/recent-submissions
Docphin | Medical Research Articles Published in November 2002 | Page 58 of 58  Docphin | Medical Research Articles Published in November 2002 | Page 58 of 58
... crystallographic studies of two C-terminally truncated copper-containing nitrite reductases from Achromobacter cycloclastes: ...
more infohttps://www.docphin.com/medical-research-articles-published-in-month?month=11&year=2002&page=58
  • 8. The pharmaceutical composition of claim 4 , wherein the cupredoxin peptide is from an organism selected from the group consisting of Pseudomonas aeruginosa, Alcaligenes faecalis, Achromobacter xylosoxidan, Bordetella bronchiseptica, Methylomonas sp. (google.com)