The type species of gram negative, aerobic bacteria in the genus ACHROMOBACTER. Previously in the genus ALCALIGENES, the classification and nomenclature of this species has been frequently emended. The two subspecies, Achromobacter xylosoxidans subsp. denitrificans and Achromobacter xylosoxidans subsp. xylosoxidans are associated with infections.
A bacterial protein from Pseudomonas, Bordetella, or Alcaligenes which operates as an electron transfer unit associated with the cytochrome chain. The protein has a molecular weight of approximately 16,000, contains a single copper atom, is intensively blue, and has a fluorescence emission band centered at 308nm.
Gram-Negative Bacterial Infections
Atomic resolution structures of resting-state, substrate- and product-complexed Cu-nitrite reductase provide insight into catalytic mechanism. (1/2)Copper-containing nitrite reductases catalyze the reduction of nitrite to nitric oxide (NO), a key step in denitrification that results in the loss of terrestrial nitrogen to the atmosphere. They are found in a wide variety of denitrifying bacteria and fungi of different physiology from a range of soil and aquatic ecosystems. Structural analysis of potential intermediates in the catalytic cycle is an important goal in understanding enzyme mechanism. Using "crystal harvesting" and substrate-soaking techniques, we have determined atomic resolution structures of four forms of the green Cu-nitrite reductase, from the soil bacterium Achromobacter cycloclastes. These structures are the resting state of the enzyme at 0.9 A, two species exhibiting different conformations of nitrite bound at the catalytic type 2 Cu, one of which is stable and also has NO present, at 1.10 A and 1.15 A, and a stable form with the product NO bound side-on to the catalytic type 2 Cu, at 1.12 A resolution. These structures provide incisive insights into the initial binding of substrate, its repositioning before catalysis, bond breakage (O-NO), and the formation of a stable NO adduct. (+info)
Assessing the impact of denitrifier-produced nitric oxide on other bacteria. (2/2)A series of experiments was undertaken to learn more about the impact on other bacteria of nitric oxide (NO) produced during denitrification. The denitrifier Rhodobacter sphaeroides 2.4.3 was chosen as a denitrifier for these experiments. To learn more about NO production by this bacterium, NO levels during denitrification were measured by using differential mass spectrometry. This revealed that NO levels produced during nitrate respiration by this bacterium were in the low muM range. This concentration of NO is higher than that previously measured in denitrifiers, including Achromobacter cycloclastes and Paracoccus denitrificans. Therefore, both 2.4.3 and A. cycloclastes were used in this work to compare the effects of various NO levels on nondenitrifying bacteria. By use of bacterial overlays, it was found that the NO generated by A. cycloclastes and 2.4.3 cells during denitrification inhibited the growth of both Bacillus subtilis and R. sphaeroides 2.4.1 but that R. sphaeroides 2.4.3 caused larger zones of inhibition in the overlays than A. cycloclastes. Both R. sphaeroides 2.4.3 and A. cycloclastes induced the expression of the NO stress response gene hmp in B. subtilis. Taken together, these results indicate that there is variability in the NO concentrations produced by denitrifiers, but, irrespective of the NO levels produced, microbes in the surrounding environment were responsive to the NO produced during denitrification. (+info)
RCSB PDB - 1NIC: THE STRUCTURE OF CU-NITRITE REDUCTASE FROM ACHROMOBACTER CYCLOCLASTES AT FIVE PH VALUES, WITH...
1NIC: The structure of copper-nitrite reductase from Achromobacter cycloclastes at five pH values, with NO2- bound and with type II copper depleted.
RCSB PDB - 1NIE: THE STRUCTURE OF CU-NITRITE REDUCTASE FROM ACHROMOBACTER CYCLOCLASTES AT FIVE PH VALUES, WITH...
1NIE: The structure of copper-nitrite reductase from Achromobacter cycloclastes at five pH values, with NO2- bound and with type II copper depleted.
The Defense for the Predators - 424 Words - DivShare
McMahons biggest defense for extinction of all carnivores is that it would be good to prevent the vast suffering and countless violent deaths caused by predation. His assumption that the end of all suffering deaths caused by predation is a good thing fails on two accounts. First, he fails to take into account predation in the microbial level. Numerous bacteria on Earth that kill other bacteria are vital to all living organisms. Virtually all, living organisms house predatory bacteria that feed on other bacteria in order to multiply. However, these bacteria also decompose harmful pathogens and aid in absorbing nutrients for survival. For example, in Humans, lactic acid bacteria kill pathogens in the stomach that cause diarrhea. In fact, the elimination of all predating microbes would wipe out all living creatures on earth due to diseases. Although according to McMahon, these causes animal suffering, most people would not desire the elimination of all predating bacteria. Suppose McMahon ...
Global Change Master Directory (GCMD)
2. Proteomics analysis has been initiated (Clare Rutherford Honours student) for analysis of response of Psychroflexus torquis to light and dark conditions grown at 2 C (grown in a modified marine medium). The proteomics involves shotgun analysis using a 2-dimensional HPLC separation of trypsinised protein extracts (recovered using the QProteome proteoin extraction kit and extraction with membrane protein surfactant C7BzO, Sigma-Aldrich) coupled to nano-flow LTQ-Orbitrap mass spectrometry. This work is done in collaboration with Dr Edwin Lowe, Central Sciences Laboratory. The goal of this experiment is to determine whether the presence of light induces PR translation (abundance) and it cognate carotenoid hydroxylase as well as other changes to the proteome. Based on recent experiments on other bacteria (E. coli and L. monocytogenes) as much as 50% of the proteome can be recovered using this approach (termed MudPit - multidimensional protein information technology). This will be first time such ...
Atomic resolution structure of human alpha-tubulin acetyltransferase bound to acetyl-CoA :: MPG.PuRe
Author: Taschner, Michael et al.; Genre: Journal Article; Published in Print: 2012-11-27; Keywords: HISTONE DEACETYLASE INHIBITORS; MICROTUBULES; SUPERFAMILY; MECHANISMS;|br/| P300/CBP; DOMAIN; SITEcilium; crystal structure; post-translational modification; ; Title: Atomic resolution structure of human alpha-tubulin acetyltransferase|br/| bound to acetyl-CoA
A Look at Pheromone Cologne
Human pheromones dont really have a strong scent. The myth that by applying deodorant, you are covering up your natural pheromones doesnt hold much water. Most people would definitely prefer the smell of deodorant over strong body odor. Technically, pheromones are odorless; they are more or less detected by an organ in your nose called the vomeronasal organ. This organ, though more developed in other mammals, sends signals to the brain when pheromones are detected that may say things like, Whoa, healthy genes at 8 oclock, lets get his number. At least thats what buyers of these products hope ...
[email protected]: Biology and potential biogeochemical impacts of novel predatory flavobacteria
Predatory bacteria are ubiquitous in aquatic environments and may be important players in the ecology and biogeochemistry of microbial communities. Three novel strains belonging to two genera of marine flavobacteria, Olleya and Tenacibaculum, were cultured from coastal sediments and found to be predatory on other bacteria on surfaces. Two published species of the genus Tenacibaculum were also observed to grow by lysis of prey bacteria, raising the possibility that predation may be a widespread lifestyle amongst marine flavobacteria, which are diverse and abundant in a variety of marine environments. The marine flavobacterial clade is known to include species capable of photoheterotrophy, scavenging of polymeric organic substances, pathogenesis on animals, the degradation and lysis of phytoplankton blooms and, now, predation on bacterial communities. Strains from the two genera were found to exhibit divergent prey specificities and growth yields when growing predatorily. Olleya sp. predatory ...
Combination of Factors that Result in Lowest Monthly Mortgage Payments
When you know the tips and options that can help in lowering your monthly mortgage payments, you are able to plan your finances more strategically
Predatory Bacterial Swarm Uses Rippling Motion to Reach Prey
Image shows swarm of M. xanthus bacteria (left) invading a colony of prey bacteria (right). The rippling pattern is the highly organized behavior of thousands of M. xanthus cells working in concert to digest the prey. Image courtesy of John Kirby, University of Iowa Carver College of Medicine Like something from a horror movie, the swarm of bacteria ripples purposefully toward their prey, devours it and moves on. Researchers at the University of Iowa are studying this behavior in Myxococcus xanthus (M. xanthus), a bacterium commonly found in soil, which preys on other bacteria.. Despite its deadly role in the bacterial world, M. xanthus is harmless to humans and might one day be used beneficially to destroy harmful bacteria on surfaces or in human infections, said John Kirby, PhD, associate professor of microbiology in the UI Roy J. and Lucille A. Carver College of Medicine. It may be that we can modify this predator-prey relationship or apply it to medically relevant situations, Kirby said. ...
WINE 101: Sulfites - Water2Wine
Any commercially-produced bottle of wine will say Contains sulfites. Our wines are all low in sulfites, but what are sulfites, and how do they effect you?
Module catalogue, Student home, The University of York
This module explores advanced aspects of protein science - including protein structure and function, determination of structure and protein engineering. Our appreciation of almost all aspects of biochemistry and molecular biology has been enhanced by the elucidation of atomic resolution structures that reveal the underlying chemical mechanisms responsible for biological function. In addition, our ability to exploit this understanding through the use of genetic approaches to engineer proteins, is leading to the generation of improved proteins for therapeutic and biotechnology applications. Students studying this module will be equipped to go on to further studies in biochemistry/molecular biology related fields of study, as well as having valuable insight into the growing biotechnology sector of industry.. ...
ProCrystal™ protein crystallization covers - Cell Culture Supplies | Sigma-Aldrich
ProCrystal™ covers are designed for high-throughput protein x-ray crystallography to generate atomic resolution structures of protein molecules. The seals sit directly over the microplate crystallization wells. The seals are validated to be incorruptible and materials will not ingress into samples. They are availablein either an individual well pre-cut format so that one can easily remove crystals using a forceps from individual wells without affecting neighboring wells or uncut format. They are exceptionally hydrophobic which maintains drop footprint and segregation even with the use of MPD, Glycerol, ordetergents.Two formats: individually cut and removable reagent well seals or single sheet, uncut sealsUV compatibleNo noticeable x-ray diffractionAccommodates up to 3 protein drops per well
HPCC Member Spotlight!
Cryo-electron microscopy is used to discover, or resolve, protein structure. This procedure is a neat tool for imaging proteins that are reluctant to crystallize. It is done by freezing a batch of proteins in liquid ethane and then imaging the non-crystalline ice. Specifically, Walls collects about 1500 micrograph images of the frozen, tumbling proteins in 48 hours. Cryo-EM uniquely enables looking at biological macromolecules in a near-native state, since they are frozen in the shapes that they take on in everyday life. With these images in tow, Walls can then use highly parallelized algorithms to solve for the proteins atomic resolution structures - ultimately a list of amino acid coordinates in three dimensions. The algorithms align, unblur, and average the 2D images of the proteins, and the more images there are of proteins at slightly different tilts and conformations, the more precise the 2D class averages will show the various orientations. The more precise these 2D classes are, the ...
- I257E was obtained by site directed mutagenesis of nitrite reductase from Achromobacter cycloclastes. (rcsb.org)
- xylosoxidans Achromobacter xylosoxidans subspecies xylosoxidans Achromobacter xylosoxidans subsp. (nih.gov)
- use ALCALIGENES 1976-2003 MH - Achromobacter cycloclastes UI - D042422 MN - B3.440.400.4188.8.131.52 MN - B3.6184.108.40.206.100 MS - A species of gram negative, aerobic, denitrifying bacteria in the genus ACHROMOBACTER. (nih.gov)
- AN - infection: coord IM with GRAM-NEGATIVE BACTERIAL INFECTIONS (IM) HN - 2004 MH - Achromobacter xylosoxidans UI - D042441 MN - B3.440.400.4220.127.116.110 MN - B3.618.104.22.168.950 MS - The type species of gram negative, aerobic bacteria in the genus ACHROMOBACTER. (nih.gov)
- denitrificans and Achromobacter xylosoxidans subsp. (nih.gov)