Acetyltransferases. Medical search

Enzymes catalyzing the transfer of an acetyl group, usually from acetyl coenzyme A, to another compound. EC 2.3.1.

Enzymes that catalyze acyl group transfer from ACETYL-CoA to HISTONES forming CoA and acetyl-histones.

Formation of an acetyl derivative. (Stedman, 25th ed)

A family of histone acetyltransferases that is structurally-related to CREB-BINDING PROTEIN and to E1A-ASSOCIATED P300 PROTEIN. They function as transcriptional coactivators by bridging between DNA-binding TRANSCRIPTION FACTORS and the basal transcription machinery. They also modify transcription factors and CHROMATIN through ACETYLATION.

Enzymes that catalyze the transfer of an acetyl group, usually from ACETYL COENZYME A, to the N-terminus of a peptide chain.

An enzyme that catalyzes the formation of O-acetylcarnitine from acetyl-CoA plus carnitine. EC 2.3.1.7.

A member of the p300-CBP transcription factor family that was initially identified as a binding partner for CAMP RESPONSE ELEMENT-BINDING PROTEIN. Mutations in CREB-binding protein are associated with RUBINSTEIN-TAYBI SYNDROME.

Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.

A member of the p300-CBP transcription factors that was originally identified as a binding partner for ADENOVIRUS E1A PROTEINS.

An N-terminal acetyltransferase subtype that consists of the Naa50p catalytic subunit, and the Naa10p and Naa15p auxiliary subunits. It has specificity for the N-terminal METHIONINE of peptides where the next amino acid in the chain is hydrophobic.

Deacetylases that remove N-acetyl groups from amino side chains of the amino acids of HISTONES. The enzyme family can be divided into at least three structurally-defined subclasses. Class I and class II deacetylases utilize a zinc-dependent mechanism. The sirtuin histone deacetylases belong to class III and are NAD-dependent enzymes.

Acetyl CoA participates in the biosynthesis of fatty acids and sterols, in the oxidation of fatty acids and in the metabolism of many amino acids. It also acts as a biological acetylating agent.

An essential amino acid. It is often added to animal feed.

Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.

An N-terminal acetyltransferase subtype that consists of the Naa10p catalytic subunit and the Naa15p auxiliary subunit. The structure of this enzyme is conserved between lower and higher eukaryotes. It has specificity for N-terminal SERINE; ALANINE; THREONINE; GLYCINE; VALINE; and CYSTINE residues and acts on nascent peptide chains after the removal of the initiator METHIONINE by METHIONYL AMINOPEPTIDASES.

The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.

A group of 6-alkyl SALICYLIC ACIDS that are found in ANACARDIUM and known for causing CONTACT DERMATITIS.

Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.

Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.

An enzyme that catalyzes the transfer of acetyl groups from ACETYL-COA to arylamines. It can also catalyze acetyl transfer between arylamines without COENZYME A and has a wide specificity for aromatic amines, including SEROTONIN. However, arylamine N-acetyltransferase should not be confused with the enzyme ARYLALKYLAMINE N-ACETYLTRANSFERASE which is also referred to as SEROTONIN ACETYLTRANSFERASE.

Compounds that inhibit HISTONE DEACETYLASES. This class of drugs may influence gene expression by increasing the level of acetylated HISTONES in specific CHROMATIN domains.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.

The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.

Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.

Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.

A mitochondrial matrix enzyme that catalyzes the synthesis of L-GLUTAMATE to N-acetyl-L-glutamate in the presence of ACETYL-COA.

Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.

A class of weak acids with the general formula R-CONHOH.

The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.

A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.

A histone deacetylase subtype that is found along with HISTONE DEACETYLASE 2; RETINOBLASTOMA-BINDING PROTEIN 4; and RETINOBLASTOMA-BINDING PROTEIN 7 as core components of histone deacetylase complexes.

The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.

DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.

The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.

The generic name for the group of aliphatic hydrocarbons Cn-H2n+2. They are denoted by the suffix -ane. (Grant & Hackh's Chemical Dictionary, 5th ed)

The repeating structural units of chromatin, each consisting of approximately 200 base pairs of DNA wound around a protein core. This core is composed of the histones H2A, H2B, H3, and H4.

Proteins involved in the assembly and disassembly of HISTONES into NUCLEOSOMES.

A histone deacetylase subtype that is found along with HISTONE DEACETYLASE 1; RETINOBLASTOMA-BINDING PROTEIN 4; and RETINOBLASTOMA-BINDING PROTEIN 7 as core components of histone deacetylase complexes.

Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.

The mechanisms effecting establishment, maintenance, and modification of that specific physical conformation of CHROMATIN determining the transcriptional accessibility or inaccessibility of the DNA.

A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.

Proteins transcribed from the E1A genome region of ADENOVIRUSES which are involved in positive regulation of transcription of the early genes of host infection.

A transcription factor that partners with ligand bound GLUCOCORTICOID RECEPTORS and ESTROGEN RECEPTORS to stimulate GENETIC TRANSCRIPTION. It plays an important role in FERTILITY as well as in METABOLISM of LIPIDS.

Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)

Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.

Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.

Established cell cultures that have the potential to propagate indefinitely.

The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.

Nucleoproteins, which in contrast to HISTONES, are acid insoluble. They are involved in chromosomal functions; e.g. they bind selectively to DNA, stimulate transcription resulting in tissue-specific RNA synthesis and undergo specific changes in response to various hormones or phytomitogens.

The region of an enzyme that interacts with its substrate to cause the enzymatic reaction.

Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.

Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.

Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.

The parts of a macromolecule that directly participate in its specific combination with another molecule.

The facilitation of biochemical reactions with the aid of naturally occurring catalysts such as ENZYMES.

Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.

Proteins found in any species of fungus.

A genetic process by which the adult organism is realized via mechanisms that lead to the restriction in the possible fates of cells, eventually leading to their differentiated state. Mechanisms involved cause heritable changes to cells without changes to DNA sequence such as DNA METHYLATION; HISTONE modification; DNA REPLICATION TIMING; NUCLEOSOME positioning; and heterochromatization which result in selective gene expression or repression.

Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.

Enzymes that catalyze the methylation of arginine residues of proteins to yield N-mono- and N,N-dimethylarginine. This enzyme is found in many organs, primarily brain and spleen.

Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.

Trans-acting transcription factors produced by retroviruses such as HIV. They are nuclear proteins whose expression is required for viral replication. The tat protein stimulates LONG TERMINAL REPEAT-driven RNA synthesis for both viral regulatory and viral structural proteins. tat stands for trans-activation of transcription.

Proteins prepared by recombinant DNA technology.

Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.

Glycosylated compounds in which there is an amino substituent on the glycoside. Some of them are clinically important ANTIBIOTICS.

Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed)

A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.

A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure and transcribes DNA into RNA. It has different requirements for cations and salt than RNA polymerase I and is strongly inhibited by alpha-amanitin. EC 2.7.7.6.

Interruption or suppression of the expression of a gene at transcriptional or translational levels.

Proteins encoded by the TAT GENES of the HUMAN IMMUNODEFICIENCY VIRUS.

Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.

The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.

Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.

The rate dynamics in chemical or physical systems.

A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.

Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.

A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.

The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.

A cell line derived from cultured tumor cells.

Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.

Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.

Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.

The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.

Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.

CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)

A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)

Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.

The aggregation of soluble ANTIGENS with ANTIBODIES, alone or with antibody binding factors such as ANTI-ANTIBODIES or STAPHYLOCOCCAL PROTEIN A, into complexes large enough to fall out of solution.

A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.

Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.

The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.

A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.

An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.

Proteins found in any species of bacterium.

The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.

Deletion of sequences of nucleic acids from the genetic material of an individual.

The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.

Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.

The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

Single chains of amino acids that are the units of multimeric PROTEINS. Multimeric proteins can be composed of identical or non-identical subunits. One or more monomeric subunits may compose a protomer which itself is a subunit structure of a larger assembly.

Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.

The functional hereditary units of FUNGI.

Prodigious substrate specificity of AAC(6')-APH(2"), an aminoglycoside antibiotic resistance determinant in enterococci and staphylococci. (1/3280)

BACKGROUND: High-level gentamicin resistance in enterococci and staphylococci is conferred by AAC(6')-APH(2"), an enzyme with 6'-N-acetyltransferase and 2"-O-phosphotransferase activities. The presence of this enzyme in pathogenic gram-positive bacteria prevents the successful use of gentamicin C and most other aminoglycosides as therapeutic agents. RESULTS: In an effort to understand the mechanism of aminoglycoside modification, we expressed AAC(6')-APH(2") in Bacillus subtilis. The purified enzyme is monomeric with a molecular mass of 57 kDa and displays both the expected aminoglycoside N-acetyltransferase and O-phosphotransferase activities. Structure-function analysis with various aminoglycosides substrates reveals an enzyme with broad specificity in both enzymatic activities, accounting for AAC(6')-APH(2")'s dramatic negative impact on clinical aminoglycoside therapy. Both lividomycin A and paromomycin, aminoglycosides lacking a 6'-amino group, were acetylated by AAC(6')-APH(2"). The infrared spectrum of the product of paromomycin acetylation yielded a signal consistent with O-acetylation. Mass spectral and nuclear magnetic resonance analysis of the products of neomycin phosphorylation indicated that phosphoryl transfer occurred primarily at the 3'-OH of the 6-aminohexose ring A, and that some diphosphorylated material was also present with phosphates at the 3'-OH and the 3"'-OH of ring D, both unprecedented observations for this enzyme. Furthermore, the phosphorylation site of lividomycin A was determined to be the 5"-OH of the pentose ring C. CONCLUSIONS: The bifunctional AAC(6')-APH(2") has the capacity to inactivate virtually all clinically important aminoglycosides through N- and O-acetylation and phosphorylation of hydroxyl groups. The extremely broad substrate specificity of this enzyme will impact on future development of aminoglycosides and presents a significant challenge for antibiotic design. (+info)

The amino-terminal C/H1 domain of CREB binding protein mediates zta transcriptional activation of latent Epstein-Barr virus. (2/3280)

Latent Epstein-Barr virus (EBV) is maintained as a nucleosome-covered episome that can be transcriptionally activated by overexpression of the viral immediate-early protein, Zta. We show here that reactivation of latent EBV by Zta can be significantly enhanced by coexpression of the cellular coactivators CREB binding protein (CBP) and p300. A stable complex containing both Zta and CBP could be isolated from lytically stimulated, but not latently infected RAJI nuclear extracts. Zta-mediated viral reactivation and transcriptional activation were both significantly inhibited by coexpression of the E1A 12S protein but not by an N-terminal deletion mutation of E1A (E1ADelta2-36), which fails to bind CBP. Zta bound directly to two related cysteine- and histidine-rich domains of CBP, referred to as C/H1 and C/H3. These domains both interacted specifically with the transcriptional activation domain of Zta in an electrophoretic mobility shift assay. Interestingly, we found that the C/H3 domain was a potent dominant negative inhibitor of Zta transcriptional activation function. In contrast, an amino-terminal fragment containing the C/H1 domain was sufficient for coactivation of Zta transcription and viral reactivation function. Thus, CBP can stimulate the transcription of latent EBV in a histone acetyltransferase-independent manner mediated by the CBP amino-terminal C/H1-containing domain. We propose that CBP may regulate aspects of EBV latency and reactivation by integrating cellular signals mediated by competitive interactions between C/H1, C/H3, and the Zta activation domain. (+info)

The histone acetylase PCAF is a phorbol-ester-inducible coactivator of the IRF family that confers enhanced interferon responsiveness. (3/3280)

Transcription factors of the interferon regulatory factor (IRF) family bind to the type I interferon (IFN)-responsive element (ISRE) and activate transcription from IFN-inducible genes. To identify cofactors that associate with IRF proteins, DNA affinity binding assays were performed with nuclear extracts prepared from tissue culture cells. The results demonstrated that the endogenous IRFs bound to the ISRE are complexed with the histone acetylases, PCAF, GCN5, and p300/CREB binding protein and that histone acetylase activities are accumulated on the IRF-ISRE complexes. By testing recombinant proteins, we show that PCAF directly binds to some but not all members of the IRF family through distinct domains of the two proteins. This interaction was functionally significant, since transfection of PCAF strongly enhanced IRF-1- and IRF-2-dependent promoter activities. Further studies showed that expression of PCAF and other histone acetylases was markedly induced in U937 cells upon phorbol ester treatment, which led to increased recruitment of PCAF to the IRF-ISRE complexes. Coinciding with the induction of histone acetylases, phorbol ester markedly enhanced IFN-alpha-stimulated gene expression in U937 cells. Supporting the role for PCAF in conferring IFN responsiveness, transfection of PCAF into U937 cells led to a large increase in IFN-alpha-inducible promoter activity. These results demonstrate that PCAF is a phorbol ester-inducible coactivator of the IRF proteins which contributes to the establishment of type I IFN responsiveness. (+info)

A novel H2A/H4 nucleosomal histone acetyltransferase in Tetrahymena thermophila. (4/3280)

Recently, we reported the identification of a 55-kDa polypeptide (p55) from Tetrahymena macronuclei as a catalytic subunit of a transcription-associated histone acetyltransferase (HAT A). Extensive homology between p55 and Gcn5p, a component of the SAGA and ADA transcriptional coactivator complexes in budding yeast, suggests an immediate link between the regulation of chromatin structure and transcriptional output. Here we report the characterization of a second transcription-associated HAT activity from Tetrahymena macronuclei. This novel activity is distinct from complexes containing p55 and putative ciliate SAGA and ADA components and shares several characteristics with NuA4 (for nucleosomal H2A/H4), a 1.8-MDa, Gcn5p-independent HAT complex recently described in yeast. A key feature of both the NuA4 and Tetrahymena activities is their acetylation site specificity for lysines 5, 8, 12, and 16 of H4 and lysines 5 and 9 of H2A in nucleosomal substrates, patterns that are distinct from those of known Gcn5p family members. Moreover, like NuA4, the Tetrahymena activity is capable of activating transcription from nucleosomal templates in vitro in an acetyl coenzyme A-dependent fashion. Unlike NuA4, however, sucrose gradient analyses of the ciliate enzyme, following sequential denaturation and renaturation, estimate the molecular size of the catalytically active subunit to be approximately 80 kDa, consistent with the notion that a single polypeptide or a stable subcomplex is sufficient for this H2A/H4 nucleosomal HAT activity. Together, these data document the importance of this novel HAT activity for transcriptional activation from chromatin templates and suggest that a second catalytic HAT subunit, in addition to p55/Gcn5p, is conserved between yeast and Tetrahymena. (+info)

In vivo and in vitro processing of the Bacillus subtilis transcript coding for glutamyl-tRNA synthetase, serine acetyltransferase, and cysteinyl-tRNA synthetase. (5/3280)

In Bacillus subtilis, the adjacent genes gltX, cysE, and cysS encoding respectively glutamyl-tRNA synthetase, serine acetyl-transferase, and cysteinyl-tRNA synthetase, are transcribed as an operon but a gltX probe reveals only the presence of a monocistronic gltX mRNA (Gagnon et al., 1994, J Biol Chem 269:7473-7482). The transcript of the gltX-cysE intergenic region contains putative alternative secondary structures forming a p-independent terminator or an antiterminator, and a conserved sequence (T-box) found in the leader of most aminoacyl-tRNA synthetase and many amino acid biosynthesis genes in B. subtilis and in other Gram-positive eubacteria. The transcription of these genes is initiated 45 nt upstream from the first codon of gltX and is under the control of a sigmaA-type promoter. Analysis of the in vivo transcript of this operon revealed a cleavage site immediately downstream from the p-independent terminator structure. In vitro transcription analysis, using RNA polymerases from Escherichia coli, B. subtilis, and that encoded by the T7 phage, in the presence of various RNase inhibitors, shows the same cleavage. This processing generates mRNAs whose 5'-end half-lives differ by a factor of 2 in rich medium, and leaves putative secondary structures at the 3' end of the gltX transcript and at the 5' end of the cysE/S mRNA, which may be involved in the stabilization of these mRNAs. By its mechanism and its position, this cleavage differs from that of the other known transcripts encoding aminoacyl-tRNA synthetases in B. subtilis. (+info)

Overexpression of spermidine/spermine N1-acetyltransferase under the control of mouse metallothionein I promoter in transgenic mice: evidence for a striking post-transcriptional regulation of transgene expression by a polyamine analogue. (6/3280)

We recently generated a transgenic mouse line overexpressing spermidine/spermine N1-acetyltransferase (SSAT) gene under its own promoter. The tissue polyamine pools of these animals were profoundly affected and the mice were hairless from early age. We have now generated another transgenic-mouse line overexpressing the SSAT gene under the control of a heavy-metal-inducible mouse metallothionein I (MT) promoter. Even in the absence of heavy metals, changes in the tissue polyamine pools indicated that a marked activation of polyamine catabolism had occurred in the transgenic animals. As with the SSAT transgenic mice generated previously, the mice of the new line (MT-SSAT) suffered permanent hair loss, but this occurred considerably later than in the previous SSAT transgenic animals. Liver was the most affected tissue in the MT-SSAT transgenic animals, revealed by putrescine overaccumulation, significant decrease in spermidine concentration and >90% reduction in the spermine pool. Even though hepatic SSAT mRNA accumulated to massive levels in non-induced transgenic animals, SSAT activity was only moderately elevated. Administration of ZnSO4 further elevated the level of hepatic SSAT message and induced enzyme activity, but not more than 2- to 3-fold. Treatment of the transgenic animals with the polyamine analogue N1,N11-diethylnorspermine (DENSPM) resulted in an immense induction, more than 40000-fold, of enzyme activity in the liver of transgenic animals, and minor changes in the SSAT mRNA level. Liver spermidine and spermine pools were virtually depleted within 1-2 days in response to the treatment with the analogue. The treatment also resulted in a marked mortality (up to 60%) among the transgenic animals which showed ultrastructural changes in the liver, most notably mitochondrial swelling, one of the earliest signs of cell injury. These results indicated that, even without its own promoter, SSAT is powerfully induced by the polyamine analogue through a mechanism that appears to involve a direct translational and/or heterogenous nuclear RNA processing control. It is likewise significant that overexpression of SSAT renders the animals extremely sensitive to polyamine analogues. (+info)

Virus infection leads to localized hyperacetylation of histones H3 and H4 at the IFN-beta promoter. (7/3280)

Transcriptional activation of the human interferon-beta (IFN-beta) gene by virus infection requires the assembly of a higher order nucleoprotein complex, the enhanceosome, which consists of the transcriptional activators NF-kappa B (p50/p65), ATF-2/c-jun, IRF-3 and IRF-7, architectural protein HMGI(Y), and the coactivators p300 and CBP. In this report, we show that virus infection of cells results in a dramatic hyperacetylation of histones H3 and H4 that is localized to the IFN-beta promoter. Furthermore, expressing a truncated version of IRF-3, which lacks a p300/CBP interaction domain, suppresses both histone hyperacetylation and activation of the IFN-beta gene. Thus, coactivator-mediated localized hyperacetylation of histones may play a crucial role in inducible gene expression. (+info)

A viral mechanism for inhibition of p300 and PCAF acetyltransferase activity. (8/3280)

Nucleosomal histone modification is believed to be a critical step in the activation of RNA polymerase II-dependent transcription. p300/CBP and PCAF histone acetyltransferases (HATs) are coactivators for several transcription factors, including nuclear hormone receptors, p53, and Stat1alpha, and participate in transcription by forming an activation complex and by promoting histone acetylation. The adenoviral E1A oncoprotein represses transcriptional signaling by binding to p300/CBP and displacing PCAF and p/CIP proteins from the complex. Here, we show that E1A directly represses the HAT activity of both p300/CBP and PCAF in vitro and p300-dependent transcription in vivo. Additionally, E1A inhibits nucleosomal histone modifications by the PCAF complex and blocks p53 acetylation. These results demonstrate the modulation of HAT activity as a novel mechanism of transcriptional regulation. (+info)

Histone acetyltransferase (HATs)[edit]. Histone Acetyltransferases, also known as HATs, are a family of enzymes that acetylate ... Marmorstein R (August 2001). "Structure of histone acetyltransferases". Journal of Molecular Biology. 311 (3): 433-44. doi: ... The process is aided by factors known as histone acetyltransferases (HATs). HAT molecules facilitate the transfer of an acetyl ... Kuo MH, Allis CD (August 1998). "Roles of histone acetyltransferases and deacetylases in gene regulation". BioEssays. 20 (8): ...

https://en.wikipedia.org/wiki/Histone_acetylation_and_deacetylation

Histone acetyltransferase 1, also known as HAT1, is an enzyme that, in humans, is encoded by the HAT1 gene. The protein encoded ... "Entrez Gene: histone acetyltransferase 1". CS1 maint: discouraged parameter (link) Olsen JV, Blagoev B, Gnad F, et al. (2006 ... 2007). "Properties of the type B histone acetyltransferase Hat1: H4 tail interaction, site preference, and involvement in DNA ... Marmorstein R (2001). "Structure of histone acetyltransferases". J. Mol. Biol. 311 (3): 433-44. doi:10.1006/jmbi.2001.4859. ...

https://en.wikipedia.org/wiki/HAT1

GAD67 and GAD65 are also regulated differently post-translationally. Both GAD65 and GAD67 are regulated via phosphorylation of a dynamic catalytic loop,[10][11] but the regulation of these isoforms differs; GAD65 is activated by phosphorylation while GAD67 is inhibited by phosphorylation. GAD67 is predominantly found activated (~92%), whereas GAD65 is predominantly found inactivated (~72%).[12] GAD67 is phosphorylated at threonine 91 by protein kinase A (PKA), while GAD65 is phosphorylated, and therefore regulated by, protein kinase C (PKC). Both GAD67 and GAD65 are also regulated post-translationally by Pyridoxal 5'-phosphate (PLP); GAD is activated when bound to PLP and inactive when not bound to PLP.[12] Majority of GAD67 is bound to PLP at any given time, whereas GAD65 binds PLP when GABA is needed for neurotransmission.[12] This reflects the functional properties of the two isoforms; GAD67 must be active at all times for normal cellular functioning, and is therefore constantly activated by ...

https://en.wikipedia.org/wiki/GAD_antibodies

A deficiency of tyrosine hydroxylase leads to impaired synthesis of dopamine as well as epinephrine and norepinephrine. It is represented by a progressive encephalopathy and poor prognosis. Clinical features include dystonia that is minimally or nonresponsive to levodopa, extrapyramidal symptoms, ptosis, miosis, and postural hypotension. This is a progressive and often lethal disorder, which can be improved but not cured by levodopa.[39] Response to treatment is variable and the long-term and functional outcome is unknown. To provide a basis for improving the understanding of the epidemiology, genotype/phenotype correlation and outcome of these diseases their impact on the quality of life of patients, and for evaluating diagnostic and therapeutic strategies a patient registry was established by the noncommercial International Working Group on Neurotransmitter Related Disorders (iNTD).[40] Additionally alterations in the tyrosine hydroxylase enzyme activity may be involved in disorders such as ...

https://en.wikipedia.org/wiki/Tyrosine_3-monooxygenase

These studies claim that ABT is a more potent inhibitor of N-acetyltransferase 2 compared with N-acetyltransferase 1. Strong JM ... N-Acetyltransferase is an enzyme that catalyzes the transfer of acetyl groups from acetyl-CoA to arylamines and aromatic amines ... Procainamide is metabolized in the liver to acecainide by N-acetyltransferase, an enzyme that is genetically determined. ... Evans DA (1989). "N-acetyltransferase". Pharmacology & Therapeutics. 42 (2): 157-234. doi:10.1016/0163-7258(89)90036-3. PMID ...

https://en.wikipedia.org/wiki/Acecainide

Choline acetyltransferase (also known as ChAT or CAT) is an important enzyme which produces the neurotransmitter acetylcholine ... Strauss WL, Kemper RR, Jayakar P, Kong CF, Hersh LB, Hilt DC, Rabin M (Feb 1991). "Human choline acetyltransferase gene maps to ... "Choline O-Acetyltransferase". GeneCards: The Human Gene Compendium. Weizmann Institute of Science. Retrieved 5 December 2013. ... Maselli RA, Chen D, Mo D, Bowe C, Fenton G, Wollmann RL (Feb 2003). "Choline acetyltransferase mutations in myasthenic syndrome ...

https://en.wikipedia.org/wiki/Transferase

A tubulin acetyltransferase is located in the axoneme, and acetylates the α-tubulin subunit in an assembled microtubule. Once ... Feng J, Hu J, Li Y, Li R, Yu H, Ma L (September 2020). "The N-Terminal Acetyltransferase Naa50 Regulates Arabidopsis Growth and ... The acetylated residue of α-tubulin is K40, which is catalyzed by α-tubulin acetyl-transferase (α-TAT) in human. The ... The identification of the crystal structure of acetylation of α-tubulin acetyl-transferase (α-TAT) also sheds a light on the ...

https://en.wikipedia.org/wiki/Acetylation

"Inhibition of Histone Acetyltransferases by CTK7A and Mthods thereof". Method for inhibiting histone acetyltransferases by ... of histone acetyltransferases". Use of certain benzoic acid and benzamide compounds as modulators of enzymes histone ... a natural histone acetyltransferase inhibitor, represses chromatin transcription and alters global gene expression". Journal of ... "Nanosphere-histone acetyltransferase (HAT) activator composition, process and methods thereof". A composition including ...

https://en.wikipedia.org/wiki/Tapas_Kumar_Kundu

Choline, in combination with acetyl-CoA, is catalyzed by the enzyme choline acetyltransferase to produce acetylcholine and ... This acetylation is catalyzed by acetyltransferases. This acetylation affects cell growth, mitosis, and apoptosis. Allosteric ...

https://en.wikipedia.org/wiki/Acetyl-CoA

An example of a selectable-marker which is also a reporter in bacteria is the chloramphenicol acetyltransferase (CAT) gene, ... Smale, S. T. (2010-05-01). "Chloramphenicol Acetyltransferase Assay". Cold Spring Harbor Protocols. 2010 (5): pdb.prot5422. doi ...

https://en.wikipedia.org/wiki/Reporter_gene

N-acetyltransferase, an enzyme; also NAT1, NAT2, etc.. *Nucleic acid test, for genetic material ...

https://en.wikipedia.org/wiki/NAT

Phosphinothricin acetyltransferase, an enzyme. *Photoacoustic tomography, by an ultrasound scan. *Picture arrangement test ...

https://en.wikipedia.org/wiki/Pat

Python F, Stocker RF (2002) Immunoreactivity against choline acetyltransferase, gamma-aminobutyric acid, histamine, octopamine ... Python, F; Stocker, Reinhard F. (2002-10-08). "Immunoreactivity against choline acetyltransferase, ?-aminobutyric acid, ...

https://en.wikipedia.org/wiki/Reinhard_F._Stocker

Sadoul K, Boyault C, Pabion M, Khochbin S (2008). "Regulation of protein turnover by acetyltransferases and deacetylases". ... Typically, these reactions are catalyzed by enzymes with histone acetyltransferase (HAT) or histone deacetylase (HDAC) activity ...

https://en.wikipedia.org/wiki/H4K8ac

MAP3K4 controls the activity of CBP histone acetyltransferase which acetylates histones H2A and H2B to maintain the trophoblast ... Typically, these reactions are catalyzed by enzymes with histone acetyltransferase (HAT) or histone deacetylase (HDAC) activity ... "Regulation of protein turnover by acetyltransferases and deacetylases". Biochimie. 90 (2): 306-12. doi:10.1016/j.biochi.2007.06 ...

https://en.wikipedia.org/wiki/H2BK5ac

Typically, these reactions are catalyzed by enzymes with histone acetyltransferase (HAT) or histone deacetylase (HDAC) activity ... "Regulation of protein turnover by acetyltransferases and deacetylases". Biochimie. 90 (2): 306-12. doi:10.1016/j.biochi.2007.06 ...

https://en.wikipedia.org/wiki/H3K36ac

Histone acetyltransferase KAT2A is the specific reader. Proteins are typically acetylated on lysine residues and this reaction ... Sadoul K, Boyault C, Pabion M, Khochbin S (2008). "Regulation of protein turnover by acetyltransferases and deacetylases". ... Typically, these reactions are catalyzed by enzymes with histone acetyltransferase (HAT) or histone deacetylase (HDAC) activity ...

https://en.wikipedia.org/wiki/H4K91ac

Starheim KK, Gevaert K, Arnesen T (April 2012). "Protein N-terminal acetyltransferases: when the start matters". Trends in ... HATs function similarly to N-terminal acetyltransferases (NATs) but their acetylation is reversible unlike in NATs. HAT ... Many coactivators have histone acetyltransferase (HAT) activity meaning that they can acetylate specific lysine residues on the ... Some coactivators also have histone acetyltransferase (HAT) activity. HATs form large multiprotein complexes that weaken the ...

https://en.wikipedia.org/wiki/Coactivator_(genetics)

Histone acetylation Sadoul K, Boyault C, Pabion M, Khochbin S (2008). "Regulation of protein turnover by acetyltransferases and ... Typically, these reactions are catalyzed by enzymes with histone acetyltransferase (HAT) or histone deacetylase (HDAC) activity ... for chromatin structure and function in a variety of eukaryotes and is catalyzed by specific histone lysine acetyltransferases ...

https://en.wikipedia.org/wiki/H4K16ac

Glycine C-acetyltransferase is a protein that in humans is encoded by the GCAT gene. The degradation of L-threonine to glycine ... "Entrez Gene: Glycine C-acetyltransferase". Jacquot C, Lanco X, Carbonnelle D, Sevestre O, Tomasoni C, Briad G, Juget M, Roussis ...

https://en.wikipedia.org/wiki/GCAT

Finally, lacA encodes Galactoside acetyltransferase. It would be wasteful to produce enzymes when no lactose is available or if ...

https://en.wikipedia.org/wiki/Lac_operon

AANAT is a gene that encodes an enzyme aralkylamine N-acetyltransferase. It is the key regulator of day-night cycle (circadian ... The protein encoded by this gene belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin ... "Entrez Gene: Aralkylamine N-acetyltransferase". "AANAT". NCBI. Retrieved 2 November 2014. CS1 maint: discouraged parameter ( ...

https://en.wikipedia.org/wiki/AANAT_(gene)

Acetylation-by HAT (histone acetyl transferase); deacetylation-by HDAC (histone deacetylase): Acetylation tends to define the ' ...

https://en.wikipedia.org/wiki/Histone_code_hypothesis

Histone acetyltransferase p300 also known as p300 HAT or E1A-associated protein p300 (where E1A = adenovirus early region 1A) ... p300 HAT functions as histone acetyltransferase that regulates transcription via chromatin remodeling, and is important in the ... It functions as histone acetyltransferase that regulates transcription of genes via chromatin remodeling by allowing histone ... "Regulation of histone acetyltransferases p300 and PCAF by the bHLH protein twist and adenoviral oncoprotein E1A". Cell. 96 (3 ...

https://en.wikipedia.org/wiki/EP300

Acetyltransferases CBP and p300 bind to the transactivation domain. AP1, STAT5 and VDR bind to C-terminal domain. Also, ETS1 ...

https://en.wikipedia.org/wiki/ETS1

The protein encoded by this gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize ... and histone acetyltransferase domain. Recent results suggest that novel CBP-mediated post-translational N-glycosylation ... activity by interaction with histone acetyltransferases". Proceedings of the National Academy of Sciences of the United States ... "The transcriptional coactivators p300 and CBP are histone acetyltransferases". Cell. 87 (5): 953-9. doi:10.1016/s0092-8674(00) ...

https://en.wikipedia.org/wiki/CREB-binding_protein

2002). "Previously uncharacterized histone acetyltransferases implicated in mammalian spermatogenesis". Proc. Natl. Acad. Sci. ...

https://en.wikipedia.org/wiki/HIST2H2AC

This gene encodes a protein containing a chromodomain and a histone acetyltransferase catalytic domain. Chromodomain proteins ... 2002). "Previously uncharacterized histone acetyltransferases implicated in mammalian spermatogenesis". Proc. Natl. Acad. Sci. ...

https://en.wikipedia.org/wiki/CDY1

... has separate acetyltransferase and E3 ubiquitin ligase domains as well as a bromodomain for interaction with other ... The acetyltransferase activity and cellular location of PCAF are regulated through acetylation of PCAF itself. PCAF may be ... It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays ... P300/CBP-associated factor (PCAF), also known as K(lysine) acetyltransferase 2B (KAT2B), is a human gene and transcriptional ...

https://en.wikipedia.org/wiki/PCAF

This review discusses our current understanding of histone acetyltransferases (HATs) or acetyltransferases (ATs): their ... Histone acetyltransferases.. Roth SY1, Denu JM, Allis CD.. Author information. 1. Department of Biochemistry and Molecular ...

https://www.ncbi.nlm.nih.gov/pubmed/11395403?dopt=Abstract

Acetyltransferase. References[edit]. *^ a b c d e f g h i Lee KK, Workman JL (April 2007). "Histone acetyltransferase complexes ... Histone acetyltransferases (HATs) are enzymes that acetylate conserved lysine amino acids on histone proteins by transferring ... Gcn5-related N-acetyltransferases (GNATs)[edit]. HATs can be grouped into several different families based on sequence homology ... The Gcn5-related N-acetyltransferase (GNAT) family includes Gcn5, PCAF, Hat1, Elp3, Hpa2, Hpa3, ATF-2, and Nut1. These HATs are ...

https://en.wikipedia.org/wiki/Histone_acetyltransferase

Structure of histone acetyltransferases.. Marmorstein R1.. Author information. 1. The Wistar Institute and the Department of ...

https://www.ncbi.nlm.nih.gov/pubmed/11492997?dopt=Abstract

... acetyltransferase Chloramphenicol acetyltransferase Serotonin N-acetyltransferase NatA Acetyltransferase NatB acetyltransferase ... Examples include: Histone acetyltransferases including CBP histone acetyltransferase Choline ... Acetyltransferase (or transacetylase) is a type of transferase enzyme that transfers an acetyl group. ... Acyltransferase Acetylation Acetyltransferases at the US National Library of Medicine Medical Subject Headings (MeSH) v t e. ...

https://en.wikipedia.org/wiki/Acetyltransferase

The following is a list of human genes that encode N-acetyltransferase enzymes: Evans DA (1989). "N-acetyltransferase". ... N-acetyltransferases are cytosolic enzymes found in the liver and many tissues of most mammalian species, except the dog and ... N-acetyltransferase (NAT) is an enzyme that catalyzes the transfer of acetyl groups from acetyl-CoA to arylamines, ... Sinclair, J.C.; Sandy, J.; Delgoda, R.; Sim, E.; Noble, M.E. (2000). "Structure of arylamine N-acetyltransferase reveals a ...

https://en.wikipedia.org/wiki/N-acetyltransferase

Diamine acetyltransferase 1, also known as spermidine/spermine-N(1)-acetyltransferase (SSAT), is an enzyme that catalyses the ... GO:0004145 diamine N-acetyltransferase activity GO:0005515 protein binding Cellular Component. No terms assigned in this ... Spermidine/spermine-N(1)-acetyltransferase: a key metabolic regulator.. Am. J. Physiol. Endocrinol. Metab. 294 E995-1010 2008 ...

http://www.ebi.ac.uk/interpro/entry/IPR032957

... Yonggang Wang,1,2 Xiao Miao,2,3 ... X. Yang, W. Yu, L. Shi et al., "HAT4, a golgi apparatus-anchored B-type histone acetyltransferase, acetylates free histone H4 ... D. Cao, Z. Wang, C.-L. Zhang et al., "Modulation of smooth muscle gene expression by association of histone acetyltransferases ... M. R. Parthun, J. Widom, and D. E. Gottschling, "The major cytoplasmic histone acetyltransferase in yeast: links to chromatin ...

https://www.hindawi.com/journals/omcl/2014/641979/ref/

Lysine acetyltransferases (KATs) and lysine deacetylases (KDACs) are involved in the regulation of lysine acetylation, a ... post-translational protein modification regulated by lysine acetyltransferases (KATs) and lysine deacetylases (KDACs; also ... Lysine acetyltransferases and lysine deacetylases as targets for cardiovascular disease. *Peng Li ORCID: orcid.org/0000-0001- ... Tafrova, J. I. & Tafrov, S. T. Human histone acetyltransferase 1 (Hat1) acetylates lysine 5 of histone H2A in vivo. Mol. Cell. ...

https://www.nature.com/articles/s41569-019-0235-9?error=cookies_not_supported&code=09c680a1-2cff-49cf-89dc-5cd95100bca7

Isolation, partial purification and characterization of an N-acetyltransferase from Streptomyces akiyoshiensis L-138 ...

https://www.uleth.ca/dspace/handle/10133/1/browse?value=Acetyltransferases+--+Purification&type=subject

Arylamine N-acetyltransferases (NATs) are cytosolic conjugating enzymes which transfer an acetyl group from acetylCoenzyme A to ... Arylamine N-acetyltransferases: from structure to function Drug Metab Rev. 2008;40(3):479-510. doi: 10.1080/03602530802186603. ... Arylamine N-acetyltransferases (NATs) are cytosolic conjugating enzymes which transfer an acetyl group from acetylCoenzyme A to ...

https://pubmed.ncbi.nlm.nih.gov/18642144/?dopt=Abstract

C. elegans glucosamine-6-phosphate N-acetyltransferase (GNA1): coenzyme A adduct. 4ag9 C. elegans glucosamine-6-phosphate N- ...

http://www.ebi.ac.uk/interpro/protein/Q17427/structures

... Cell. 1996 Nov 29;87(5):953-9. doi: 10.1016/s0092- ... p300/CBP represents a novel class of acetyltransferases in that it does not have the conserved motif found among various other ... Here, we demonstrate that p300/CBP is not only a transcriptional adaptor but also a histone acetyltransferase. ... One such factor, the cellular p300/CBP associated factor (PCAF), possesses intrinsic histone acetyltransferase activity. ...

https://pubmed.ncbi.nlm.nih.gov/8945521/?dopt=Abstract

Gene Ontology Term: Hpa2 acetyltransferase complex. GO ID. GO:1990331 Aspect. Cellular Component. Description. A tetrameric ... protein complex capable of acetyltransferase activity. It can catalyze the transfer of an acetyl group from acetyl-CoA to an ...

https://www.yeastgenome.org/go/GO:1990331

acetyl-CoA:histone acetyltransferase activity, H2A/H2B histone acetyltransferase activity, H3/H4 histone acetyltransferase ... H4/H2A acetyltransferase activity, histone acetokinase activity, histone acetylase activity, histone lysine acetyltransferase ... Gene Ontology Term: histone acetyltransferase activity. GO ID. GO:0004402 Aspect. Molecular Function. Description. Catalysis of ... activity, histone transacetylase activity, nucleosome-histone acetyltransferase activity, acetyl-CoA:histone acetyltransferase ...

https://www.yeastgenome.org/go/GO:0004402

Histone acetyltransferase type B subunit 2. B, E. 401. Saccharomyces cerevisiae S288C. Mutation(s): 1 Gene Names: HAT2, YEL056W ... Histone acetyltransferase type B catalytic subunit. A, D. 320. Saccharomyces cerevisiae S288C. Mutation(s): 0 Gene Names: HAT1 ... Previous studies have established that the histone acetyltransferase (HAT) complex Hat1p/Hat2p medicates this modification. ...

https://www.rcsb.org/structure/4PSX

Feller, Christian (2014): Systematic analysis of lysine acetyltransferases. Dissertation, LMU München: Faculty of Biology ...

https://edoc.ub.uni-muenchen.de/18220/

The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.

http://www.rcsb.org/pdb/protein/P62577

Compare dihydrolipoamide S-acetyltransferase ELISA Kits from leading suppliers on Biocompare. View specifications, prices, ... dihydrolipoamide S-acetyltransferase ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a widely used application for ... Human Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, DLAT ELISA Kit ... Your search returned 29 dihydrolipoamide S-acetyltransferase ELISA ELISA Kit across 9 suppliers. ...

https://www.biocompare.com/pfu/110627/soids/2-320761/ELISA_Kit/ELISA_dihydrolipoamide_S-acetyltransferase

Compare carnitine acetyltransferase ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, ... carnitine acetyltransferase ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established antibody-based tool ... Your search returned 59 carnitine acetyltransferase ELISA ELISA Kit across 8 suppliers. ...

https://www.biocompare.com/pfu/110627/soids/2-55717/ELISA_Kit/ELISA_carnitine_acetyltransferase

Rabbit polyclonal Serotonin N-acetyltransferase antibody (ab3506) validated for WB and tested in Rat. Immunogen corresponding ... Anti-Serotonin N-acetyltransferase antibody. See all Serotonin N-acetyltransferase primary antibodies. ... Pineal gland serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate ... Belongs to the acetyltransferase family. AANAT subfamily.. Contains 1 N-acetyltransferase domain. ...

http://www.abcam.com/serotonin-n-acetyltransferase-antibody-ab3506.html

TIP60 is party of the MYST family of acetyltransferases conserved from yeast to humans. It acetylates core histones H2A, H3, ... The acetyltransferase domains in these enzymes also uncovered autoacetylation activity. Autoacetylation of these enzymes causes ... Structural Biochemistry/Enzyme/Acetyltransferase. From Wikibooks, open books for an open world ... p300 and CREB binding protein (CBP) are both acetyltransferases that are highly conserved. Both have specific domains within ...

https://en.wikibooks.org/wiki/Structural_Biochemistry/Enzyme/Acetyltransferase

Formate acetyltransferase 1. Details. Name. Formate acetyltransferase 1. Kind. protein. Organism. Escherichia coli (strain K12) ... Formate acetyltransferase 1. P09373. Details. Drug Relations. Drug Relations. DrugBank ID. Name. Drug group. Pharmacological ...

https://www.drugbank.ca/bio_entities/BE0001741

Exopolysaccharide (EPS) adhesins are important determinants of bacterial surface colonization and biofilm formation. Biofilms are a major cause of chronic infections and are responsible for biofouling on water-exposed surfaces. To tackle these problems, it is essential to dissect the processes leading to surface colonization at the molecular and cellular levels. Here we describe a novel c-di-GMP effector, HfsK, that contributes to the... ...

https://mbio.asm.org/keyword/arylamine-n-acetyltransferase

sp,P07097,THIL_ZOORA Acetyl-CoA acetyltransferase OS=Zoogloea ramigera GN=phaA PE=1 SV=4 ...

http://www.uniprot.org/uniprot/P07097

References for Abcams Human Histone acetyltransferase MYST3 peptide (ab41910). Please let us know if you have used this ...

http://www.abcam.com/human-histone-acetyltransferase-myst3-peptide-ab41910-references.html

Edith Sim on Arylamine N-acetyltransferases 3, part of a collection of multimedia lectures. ... Arylamine N-acetyltransferases. Part 3 Im Edith Sim, and I have been working on these enzymes for over 20 years. ... Sim, E. (2016, July 28). Arylamine N-acetyltransferases 3 [Video file]. In The Biomedical & Life Sciences Collection, Henry ... Arylamine N-acetyltransferases 3. Part 3 of 3 *Part 1 of 3 ... Arylamine N-acetyltransferases (recap). *Nat in fungi ...

https://hstalks.com/t/3320/arylamine-n-acetyltransferases-3/?biosci

α-tubulin acetyltransferase (αTAT1) is the major α-tubulin lysine-40 (K40) acetyltransferase in mammals, nematodes, and ... Structure of the α-tubulin acetyltransferase αTAT1. David R. Friedmann, Andrea Aguilar, Jiayi Fan, Maxence V. Nachury, Ronen ... Structure of the α-tubulin acetyltransferase αTAT1. David R. Friedmann, Andrea Aguilar, Jiayi Fan, Maxence V. Nachury, Ronen ... B and C) The ability of the αTAT1 mutants to acetylate microtubules was assessed by using an in vitro acetyltransferase assay. ...

https://www.pnas.org/content/109/48/19655.long

Study finds N-alpha-acetyltransferase D (NatD) promotes lung cancer progression. Nanjing University School of Life Sciences ...

https://eurekalert.org/pub_releases/2017-10/nuso-sfn101217.php

2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB. Proc Natl Acad Sci USA ... 2016) First things first: Vital protein marks by N-terminal acetyltransferases. Trends Biochem Sci 41:746-760. ... NAA80 is actins N-terminal acetyltransferase and regulates cytoskeleton assembly and cell motility. Adrian Drazic, Henriette ... Here, we describe the discovery of actins N-terminal acetyltransferase (NAT), NAA80. We further demonstrate that actin Nt- ...

https://www.pnas.org/content/115/17/4399

... Prija Ponnan,1,2 Ajit Kumar,1,3 Prabhjot ... S. Y. Roth, J. M. Denu, and C. D. Allis, "Histone acetyltransferases," Annual Review of Biochemistry, vol. 70, pp. 81-120, 2001 ... K. K. Lee and J. L. Workman, "Histone acetyltransferase complexes: one size doesnt fit all," Nature Reviews Molecular Cell ... J. A. G. Agúndez, "Polymorphisms of human N-acetyltransferases and cancer risk," Current Drug Metabolism, vol. 9, no. 6, pp. ...

https://www.hindawi.com/journals/tswj/2014/578956/ref/

One such factor, the cellular p300/CBP associated factor (PCAF), possesses intrinsic histone acetyltransferase activity. (nih.gov)
Here, we demonstrate that p300/CBP is not only a transcriptional adaptor but also a histone acetyltransferase. (nih.gov)
p300/CBP represents a novel class of acetyltransferases in that it does not have the conserved motif found among various other acetyltransferases. (nih.gov)
p300 and CREB binding protein (CBP) are both acetyltransferases that are highly conserved. (wikibooks.org)
CBP/p300 phosphorylation activates acetyltransferase activity, which increases histone acetylation at target promoters and facilitates CBP/p300-dependent transactivation of genes. (wikibooks.org)
Here we describe a programmable, CRISPR-Cas9-based acetyltransferase consisting of the nuclease-null dCas9 protein fused to the catalytic core of the human acetyltransferase p300. (nature.com)
C646 is an inhibitor for histone acetyltransferase , and inhibits p300 with a K i of 400 nM in a cell-free assay. (selleckchem.com)
Preferentially selective for p300 versus other acetyltransferases. (selleckchem.com)
Histone Acetyltransferase Inhibitor II (HAT Inhibitor II, compound 2c) is a potent, selective and cell-permeable p300 histone acetyltransferase (HAT) inhibitor with IC50 of 5 μM. (selleckchem.com)
P300/CBP-IN-3 is an inhibitor of p300/CBP histone acetyltransferase. (selleckchem.com)
Inhibitors of histone acetyltransferases (HATs, also known as lysine acetyltransferases), such as CBP/p300, are potential therapeutics for treating cancer. (jove.com)
Both CBP/p300 and p/CAF exhibit strong histone acetyltransferase (HAT) activities ( 9 , 11 ), whereas the p160 factors possess weak COOH-terminal HAT activity ( 8 , 12 ). (sciencemag.org)
Here we show the transcriptional coactivators and histone acetyltransferases, p300/CBP, PCAF, and ACTR associate with the bHLH-PAS proteins, CLOCK and NPAS2, to regulate positively clock gene expression. (ad-astra.ro)
Mechanistically, the cytokine, while not affecting the HNF1α DNA-binding capacity, impaired its ability to recruit CBP/p300 acetyltransferases on target gene promoters and, consequently, its transactivating function. (frontiersin.org)
Activation of these factors is mediated, in part, through acetylation controlled by histone deacetylases and an intrinsic histone acetyltransferase (HAT), p300. (ahajournals.org)
To discover a potential anticancer effect of p300/CBP inhibition, we used two different approaches: (i) downregulation of p300 and CBP by specific short interfering RNA (siRNA) and (ii) chemical inhibition of the acetyltransferase activity by a newly developed small molecule, C646. (aacrjournals.org)
The histone acetyltransferases (HAT) CBP and p300 are important coactivators favoring transcription. (aacrjournals.org)
Following mitogen stimulation, S6Ks interact with the p300 and p300/CBP-associated factor (PCAF) acetyltransferases. (diva-portal.org)

Ferguson RJ, Doll MA, Rustan TD, Gray K, Hein DW: Cloning, expression, and functional characterization of two mutant (NAT2(191) and NAT2(341/803)) and wild-type human polymorphic N-acetyltransferase (NAT2) alleles. (drugbank.ca)
The xenobiotic metabolizing enzyme, mouse arylamine N -acetyltransferase type 2 ( Nat2 ), is expressed during embryogenesis from the blastocyst stage and in the developing neural tube and eye. (springer.com)
2000) Chromosome mapping of the genes for murine arylamine N -acetyltransferases (NATs), enzymes involved in the metabolism of carcinogens: identification of a novel upstream noncoding exon for murine Nat2. (springer.com)
Neither N-acetyltransferase 1 (NAT1) nor N-acetyltransferase 2 (NAT2) genotype alone was associated with increased breast cancer risk. (aacrjournals.org)
Genetically polymorphic xenobiotic metabolizing enzymes such as the polymorphic arylamine N-acetyltransferase (NAT2) are supposed to be a host factor for cancer susceptibility. (hu-berlin.de)
Arylamine N -acetyltransferase 2 (NAT2) is involved in both the detoxification and bioactivation of carcinogenic arylamines and other mutagens. (aacrjournals.org)
Background -Arylamine N -acetyltransferases in humans (NAT1 and NAT2) catalyse the acetylation of arylamines including food derived heterocyclic arylamine carcinogens. (bmj.com)
There are two closely related genes on chromosome 8 that encode the two human arylamine N -acetyltransferases-NAT1 and NAT2. (aspetjournals.org)
Humans possess two functional N -acetyltransferase genes, NAT1 and NAT2 , as well as a nonfunctional pseudogene, NATP . (aspetjournals.org)
Background: N-acetyltransferase 2 (NAT2) metabolizes arylamines and hydrazines moeities found in many therapeutic drugs, chemicals and carcinogens. (fraunhofer.de)
The presentations focused on the pharmacogenetics of the NAT1 and NAT2 arylamine N -acetyltransferases, including developmental regulation, structure-function relationships, and their possible role in susceptibility to breast, colon, and pancreatic cancers. (aspetjournals.org)

Acetyltransferase (or transacetylase) is a type of transferase enzyme that transfers an acetyl group. (wikipedia.org)
N-acetyltransferase (NAT) is an enzyme that catalyzes the transfer of acetyl groups from acetyl-CoA to arylamines, arylhydroxylamines and arylhydrazines. (wikipedia.org)
Diamine acetyltransferase 1, also known as spermidine/spermine-N(1)-acetyltransferase (SSAT), is an enzyme that catalyses the acetylation of polyamines. (ebi.ac.uk)
Pineal gland serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AA-NAT) is an ~23 kDa protein which is the rate-limiting enzyme in melatonin synthesis. (abcam.com)
An acetyltransferase is an enzyme that catalyzes the transfer of acetyl groups. (wikibooks.org)
Both have specific domains within the enzyme: three cysteine-histidine rich domains, bromodomain, and acetyltransferase domain. (wikibooks.org)
In this study we examined the developmental roles of acetylcholine (ACh) by establishing and analyzing mice lacking choline acetyltransferase (ChAT), the biosynthetic enzyme for ACh. (jneurosci.org)
Short-chain carnitine conjugates, including acetylcarnitine, derive from their corresponding acyl-CoA precursors via the action of carnitine acetyltransferase (CrAT), a bidirectional mitochondrial matrix enzyme. (rti.org)
The systematic name of this enzyme class is acetyl-CoA:alcohol O-acetyltransferase . (wikidoc.org)
This enzyme is also called alcohol acetyltransferase . (wikidoc.org)
Atmane N, Dairou J, Paul A, Dupret JM, Rodrigues-Lima F (2003) Redox regulation of the human xenobiotic metabolizing enzyme arylamine N -acetyltransferase 1 (NAT1). (springer.com)
Molecular model of a complex formed between the serotonin N-acetyltransferase enzyme (AANAT, light blue, light red) and the zeta isoform structure of a 14-3-3 protein (yellow, green). (sciencephoto.com)
Furthermore, the identification of phytosphingosine as substrate for alcohol acetyltransferase Atf2p broadens the known substrate range of this enzyme. (biomedsearch.com)
An obsolete generic term for any enzyme (e.g., acetyl CoA acetyltransferase [EC 2.3.1.9]) which catalyses a reaction between a compound, R-R', and a thiol, R'-SH, forming R-S-R' and R'-H. (thefreedictionary.com)
This reaction is catalyzed by the enzyme ornithine acetyltransferase, which is a product of the argJ gene. (asm.org)
In the current study, we report that Aβ peptides, in particular Aβ42, allosterically enhances the catalytic rate of the core-cholinergic enzyme choline acetyltransferase (ChAT), responsible for biosynthesis of ACh. (frontiersin.org)

Choline acetyltransferase mutations cause myasthenic syndrome associated with episodic apnea in humans. (sigmaaldrich.com)
Murine choline-O-acetyltransferase (EC 2.3.1.6) is a 71,721 dalton protein(640 amino acids) expressed in cholinergic neurons of both the PNS and CNS. (neuromics.com)
Choline O-Acetyltransferase" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
This graph shows the total number of publications written about "Choline O-Acetyltransferase" by people in this website by year, and whether "Choline O-Acetyltransferase" was a major or minor topic of these publications. (umassmed.edu)
Below are the most recent publications written about "Choline O-Acetyltransferase" by people in Profiles. (umassmed.edu)

In contrast to previous dCas9-based activators, the acetyltransferase activates genes from enhancer regions and with an individual guide RNA. (nature.com)
Ebisawa T, Deguchi T: Structure and restriction fragment length polymorphism of genes for human liver arylamine N-acetyltransferases. (drugbank.ca)
Acetylation of phytosphingosine in W. ciferrii is catalyzed by acetyltransferases Sli1p and Atf2p, encoded by genes similar to Saccharomyces cerevisiae YGR212W and YGR177C, respectively. (biomedsearch.com)
In this study, we identified and characterized a third rat N -acetyltransferase gene ( Nat3 ) consisting of a single open reading frame of 870 base pairs encoding a 290-amino acid protein, analogous to the previously identified human and rat N -acetyltransferase genes. (aspetjournals.org)
2 N -Acetyltransferase proteins and genes are printed in regular type (NAT) and italics ( NAT ), respectively. (aspetjournals.org)

Arylamine N-acetyltransferases (NATs) are cytosolic conjugating enzymes which transfer an acetyl group from acetylCoenzyme A to a xenobiotic acceptor substrate. (nih.gov)
In this study, we examined the involvement of a specific gene family, encoding protein N-terminal acetyltransferases (NATs), in various types of tumours by analysing available large-scale cancer-associated datasets. (mdpi.com)
N-terminal acetylation, catalysed by N-terminal acetyltransferases (NATs), is among the most common protein modifications in eukaryotes and involves the transfer of an acetyl group from acetyl-CoA to the α-amino group of the first amino acid. (portlandpress.com)
In eukaryotes, there are up to six N-terminal acetyltransferases (NATs), termed NatA-NatF, serving as the catalytic machinery for Nt-acetylation. (portlandpress.com)
N -terminal acetyltransferase (Nats) complex is responsible for protein N -terminal acetylation ( N α-acetylation), which is one of the most common covalent modifications of eukaryotic proteins. (mdpi.com)
it mainly occurs cotranslationally on nascent polypeptide chains and almost all N α-acetylation is catalyzed by the action of ribosome associated N -terminal acetyltransferase (Nats) complex in eukaryotes [ 8 ]. (mdpi.com)

A tetrameric protein complex capable of acetyltransferase activity. (yeastgenome.org)
N-terminal acetylation (Nt-Ac) is an abundant eukaryotic protein modification, deposited in humans by one of seven N-terminal acetyltransferase (NAT) complexes composed of a catalytic and potentially auxiliary subunits. (mdpi.com)
The translocation t(8;16)(p11;p13) of acute myeloid leukaemia fuses a putative acetyltransferase to the CREB-binding protein. (springer.com)
Identification of a human histone acetyltransferase related to monocytic leukemia zinc finger protein. (springer.com)
The monocytic leukemia zinc finger protein MOZ is a histone acetyltransferase. (springer.com)
Zusätzlich bieten wir Ihnen MYST Histone Acetyltransferase 2 Proteine (8) und MYST Histone Acetyltransferase 2 Kits (3) und viele weitere Produktgruppen zu diesem Protein an. (antikoerper-online.de)
NAT10 (N-acetyltransferase 10) is a nucleolar protein, but may show subcellular redistribution in colorectal carcinoma. (aacrjournals.org)
We found that N-acetyltransferase 10 (NAT10), which is a nucleolar protein, undergoes subcellular redistribution from the nucleolus in normal tissues to the cytoplasm and membrane in colorectal carcinoma cells. (aacrjournals.org)
The SAGA (Spt-Ada-Gcn5-acetyltransferase) complex contains several subunits which also function as part of other protein complexes, including a subset of TATA box binding protein-associated factors (TAFIIs) and Tra1. (asm.org)

Your search returned 29 dihydrolipoamide S-acetyltransferase ELISA ELISA Kit across 9 suppliers. (biocompare.com)

SMMC-7721 cells were treated with EPZ-6438 (1 μM) alone or in combination with histone acetyltransferase inhibitors C646 (5 μM) or SGC-CBP30 (5 μM) for 6 days. (selleckchem.com)
Click on the product name to view detailed information such as the chemical structure and specific chemical properties for each of our Carnitine Acetyltransferase Inhibitors. (scbt.com)
In stock Carnitine Acetyltransferase Inhibitors are available for immediate shipping. (scbt.com)
Acetanilide and derivatives of benzoic acid, benzamide, salicylic acid, and salicylamide were tested as inhibitors of p-aminobenzoic acid N-acetyltransferase partially purified from peripheral blood of three rapid and two slow dapsone acetylator human volunteers. (aspetjournals.org)
On the other hand, histone acetyltransferase (HAT) inhibitors have been less extensively investigated for their potential use in cancer therapy. (aacrjournals.org)

Grant DM, Lottspeich F, Meyer UA: Evidence for two closely related isozymes of arylamine N-acetyltransferase in human liver. (drugbank.ca)

Acetylation is catalyzed by histone acetyltransferases (HATs), which add acetyl groups to lysine residues in their targets. (jimmunol.org)

Histone acetyltransferases ( HATs ) are enzymes that acetylate conserved lysine amino acids on histone proteins by transferring an acetyl group from acetyl-CoA to form ε- N -acetyllysine. (wikipedia.org)
Histone acetyltransferases can also acetylate non-histone proteins, such as nuclear receptors and other transcription factors to facilitate gene expression. (wikipedia.org)
The steady-state level of acetylation of histone proteins is accomplished by the action of histone acetyltransferases (HATs) and histone deacetylases (HDACs) ( 37 ). (asm.org)

Deguchi T: Sequences and expression of alleles of polymorphic arylamine N-acetyltransferase of human liver. (drugbank.ca)

Here we report the molecular characterization of the αTAT1 acetyltransferase. (pnas.org)
Characterization of serine acetyltransferase (CysE) from methicillin-resistant Staphylococcus aureus and inhibitory effect of two natural products on CysE. (bioportfolio.com)

We observed that ATAT1 tubulin acetyltransferase binds and regulates cortactin acetylation levels. (archives-ouvertes.fr)

This review discusses our current understanding of histone acetyltransferases (HATs) or acetyltransferases (ATs): their discovery, substrate specificity, catalytic mechanism, regulation, and functional links to transcription, as well as to other chromatin-modifying activities. (nih.gov)
An N-terminal acetyltransferase subtype that consists of the Naa10p Catalytic Subunit and the Naa15p auxiliary subunit. (online-medical-dictionary.org)
5 ) defined Gcn5 as the catalytic subunit of a transcriptional coactivation complex SAGA (Spt-Ada-Gcn5-acetyltransferase) that is involved in regulating gene transcription in different tissues and organs. (jimmunol.org)
An N-terminal acetyltransferase subtype that consists of the Naa50p Catalytic Subunit , and the Naa10p and Naa15p auxiliary subunits. (online-medical-dictionary.org)
Recent findings in humans indicate that a dysfunctional Nα-acetyltransferase (Naa) 10, the catalytic subunit of NatA, the major NAT, is associated with lethality during infancy. (portlandpress.com)
The serine acetyltransferase activity of CysE was detected and its catalytic properties were also determined. (bioportfolio.com)

Histone Acetyltransferase Inhibitor II shows anti-acetylase activity in mammalian cells. (selleckchem.com)
CPTH2 is a potent histone acetyltransferase (HAT) inhibitor modulating Gcn5p network. (selleckchem.com)
1-Aminobenzotriazole is also a substrate and inhibitor of N-acetyltransferase (NAT) . (selleckchem.com)
WM-8014 is a highly potent inhibitor of histone acetyltransferase KAT6A with an IC50 of 8 nM. (selleckchem.com)
WM-1119 is a highly potent, selective inhibitor of lysine acetyltransferase KAT6A with IC50 of 0.25 μM. (selleckchem.com)
In addition to its function as an inhibitor of histone acetyltransferases, Nir (Noc2l) binds to p53 and TAp63 to regulate their activity. (biologists.org)

Your search returned 59 carnitine acetyltransferase ELISA ELISA Kit across 8 suppliers. (biocompare.com)
Biochemicals that inhibit Carnitine Acetyltransferase have many applications in biochemical and physiological research. (scbt.com)

Other names in common use include acetoacetyl-CoA thiolase , beta-acetoacetyl coenzyme A thiolase , 2-methylacetoacetyl-CoA thiolase , 3-oxothiolase , acetyl coenzyme A thiolase , acetyl-CoA acetyltransferase , acetyl-CoA:N-acetyltransferase , and thiolase II . (primidi.com)

Synthetic peptide corresponding to Rat Serotonin N-acetyltransferase aa 195-205 (C terminal). (abcam.com)
Detects a band of approximately 23 kDa.Can be blocked with Serotonin N-acetyltransferase peptide (ab5004) . (abcam.com)

Lysine acetyltransferase 8 (KAT8) is critical for acetylation of histone H4 at lysine 16 (H4K16), an evolutionarily conserved epigenetic mark. (jci.org)

α-tubulin acetyltransferase (αTAT1) is the major α-tubulin lysine-40 (K40) acetyltransferase in mammals, nematodes, and protozoa, and its activity plays a conserved role in several microtubule-based processes. (pnas.org)
This modification is considered a hallmark of stable, long-lived microtubules ( 7 ) and α-tubulin acetyltransferase (αTAT1)/Mec-17 was recently identified to acetylate K40 on α-tubulin, with no detectable acetylation of histone substrates ( 9 , 10 ). (pnas.org)
Molecular basis for age-dependent microtubule acetylation by tubulin acetyltransferase. (ad-astra.ro)

Thus, transcription factor-specific differences in configuration and content of the coactivator complex dictate requirements for specific acetyltransferase activities, providing an explanation, at least in part, for the presence of multiple HAT components of the complex. (sciencemag.org)
Component of the HBO1 complex which has a histone H4- specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. (antikoerper-online.de)

Histone acetyltransferases (HATs) and histone deacetylases (HDACs) maintain the homeostasis of histone acetylation. (gc.ca)
Histone acetyltransferases (HATs) regulate inducible transcription in multiple cellular processes and during inflammatory and immune response. (jimmunol.org)

Structural and mechanistic insights into regulation of HBO1 histone acetyltransferase (zeige HAT Antikörper ) activity by BRPF2 (zeige BRD1 Antikörper ) have been presented. (antikoerper-online.de)
Further insight into N -acetyltransferase function, expression, and regulation may predict individual incidences of pharmaceutical drug toxicities or individual tissue-specific cancer susceptibilities associated with environmental and/or occupational arylamine procarcinogen exposures. (aspetjournals.org)
The finding that S6Ks are targeted by histone acetyltransferases uncovers a novel mode of crosstalk between mitogenic signalling pathways and the transcriptional machinery and reveals additional complexity in the regulation of S6K function. (diva-portal.org)

The acetyltransferase domains in these enzymes also uncovered autoacetylation activity. (wikibooks.org)
Mellert, Hestia S. and McMahon, Steven B. "Biochemical pathways that regulate acetyltransferase and deacetylase activity in mammalian cells. (wikibooks.org)
1996 ). A few years later, the acetyltransferase activity was demonstrated and mapped to the MYST domain (Champagne et al. (springer.com)
KAT7 -containing acetyltransferases associating with the Mis18 complex provides competence for histone turnover/exchange activity on alphoid DNA and prevents Suv39h1 (zeige SUV39H1 Antikörper )-mediated heterochromatin invasion into centromeres. (antikoerper-online.de)
Predicted to have N-acetyltransferase activity. (mcw.edu)
EGCG suppresses prostate cancer cell growth modulating acetylation of androgen receptor by anti-histone acetyltransferase activity. (sigmaaldrich.com)
Taken together, our data suggest that Salmonella may use SseF to exploit the host TIP60 acetyltransferase activity to promote efficient Salmonella replication inside host cells. (purdue.edu)
Docking data showed that residues Asp94 and His95 were essential for serine acetyltransferase activity of CysE, which were confirmed by site-directed mutagenesis. (bioportfolio.com)

The human arylamine N -acetyltransferases first attracted attention because of their role in drug metabolism. (aspetjournals.org)

TIP60 is party of the MYST family of acetyltransferases conserved from yeast to humans. (wikibooks.org)
Schizosaccharomyces pombe Mst1 is a member of the MYST family of histone acetyltransferases and is the likely ortholog of Saccharomyces cerevisiae Esa1 and human Tip60 (KAT5). (genetics.org)
Involvement of TIP60 Acetyltransferase in Intracellular Salmonella Rep" by Xueqin Wang, Dongju Li et al. (purdue.edu)
Involvement of TIP60 Acetyltransferase in Intracellular Salmonella Replication. (purdue.edu)
We identified that TIP60, an acetyltransferase, interacts with SseF. (purdue.edu)

N-acetyltransferases are cytosolic enzymes found in the liver and many tissues of most mammalian species, except the dog and fox, which cannot acetylate xenobiotics.Acetyl groups are important in the conjugation of metabolites from the liver, to allow excretion of the byproducts (phase II metabolism). (wikipedia.org)

We find that αTAT1 has an overall fold similar to the Gcn5 histone acetyltransferase but contains a relatively wide substrate binding groove and unique structural elements that play important roles in α-tubulin-specific acetylation. (pnas.org)
We have identified two Gcn5-dependent histone acetyltransferase (HAT) complexes from Saccharomyces cerevisiae , the 0.8-MDa ADA complex and the 1.8-MDa SAGA complex. (asm.org)

Dairou J, Malecaze F, Dupret JM, Rodrigues-Lima F (2005) The xenobiotic-metabolizing enzymes arylamine N -acetyltransferases in human lens epithelial cells: inactivation by cellular oxidants and UVB-induced oxidative stress. (springer.com)

Arylamine N -acetyltransferases metabolise a range of hydrazines and arylamines (see Weber and Hein 1 for review). (bmj.com)

Three in vitro methods for validation include HAT assays with recombinant acetyltransferases, immunoblotting for histone acetylation in cell culture, and ChIP-qPCR. (jove.com)

Grant DM, Blum M, Demierre A, Meyer UA: Nucleotide sequence of an intronless gene for a human arylamine N-acetyltransferase related to polymorphic drug acetylation. (drugbank.ca)
Ohsako S, Deguchi T: Cloning and expression of cDNAs for polymorphic and monomorphic arylamine N-acetyltransferases from human liver. (drugbank.ca)
Vatsis KP, Martell KJ, Weber WW: Diverse point mutations in the human gene for polymorphic N-acetyltransferase. (drugbank.ca)
My own interest is/was arylamine N-acetyltransferases involved in xenobiotic metabolism and the sequences of the two human forms (and variants) and rabbit forms have been available for some time. (bio.net)
Inhibition of human red blood cell N-acetyltransferase. (aspetjournals.org)

Acetyl-CoA C-acetyltransferase belongs to the thiolase family of enzymes. (primidi.com)

Spermidine/spermine-N(1)-acetyltransferase: a key metabolic regulator. (ebi.ac.uk)

Anatomy8

Organisms2

Chemicals and Drugs51

Analytical, Diagnostic and Therapeutic Techniques and Equipment12

Phenomena and Processes39

Information Science3

Prodigious substrate specificity of AAC(6')-APH(2"), an aminoglycoside antibiotic resistance determinant in enterococci and staphylococci. (1/3280)

The amino-terminal C/H1 domain of CREB binding protein mediates zta transcriptional activation of latent Epstein-Barr virus. (2/3280)

The histone acetylase PCAF is a phorbol-ester-inducible coactivator of the IRF family that confers enhanced interferon responsiveness. (3/3280)

A novel H2A/H4 nucleosomal histone acetyltransferase in Tetrahymena thermophila. (4/3280)

In vivo and in vitro processing of the Bacillus subtilis transcript coding for glutamyl-tRNA synthetase, serine acetyltransferase, and cysteinyl-tRNA synthetase. (5/3280)

Overexpression of spermidine/spermine N1-acetyltransferase under the control of mouse metallothionein I promoter in transgenic mice: evidence for a striking post-transcriptional regulation of transgene expression by a polyamine analogue. (6/3280)

Virus infection leads to localized hyperacetylation of histones H3 and H4 at the IFN-beta promoter. (7/3280)

A viral mechanism for inhibition of p300 and PCAF acetyltransferase activity. (8/3280)

Acetyltransferases

Histone Acetyltransferases

Acetylation

p300-CBP Transcription Factors

N-Terminal Acetyltransferases

Carnitine O-Acetyltransferase

CREB-Binding Protein

Histones

E1A-Associated p300 Protein

N-Terminal Acetyltransferase E

Histone Deacetylases

Acetyl Coenzyme A

Lysine

Saccharomyces cerevisiae Proteins

N-Terminal Acetyltransferase A

Chromatin

Anacardic Acids

Transcription Factors

Trans-Activators

Arylamine N-Acetyltransferase

Histone Deacetylase Inhibitors

Amino Acid Sequence

Nuclear Proteins

Molecular Sequence Data

Substrate Specificity

Transcription, Genetic

Cell Cycle Proteins

Transcriptional Activation

Amino-Acid N-Acetyltransferase

Protein Processing, Post-Translational

Hydroxamic Acids

Protein Binding

Saccharomyces cerevisiae

Histone Deacetylase 1

Protein Structure, Tertiary

Promoter Regions, Genetic

Sequence Homology, Amino Acid

Alkanes

Nucleosomes

Histone Chaperones

Histone Deacetylase 2

Mutation

Chromatin Assembly and Disassembly

Chromatin Immunoprecipitation

Adenovirus E1A Proteins

Nuclear Receptor Coactivator 2

Cell Nucleus

Gene Expression Regulation, Enzymologic

DNA-Binding Proteins

Cell Line

HeLa Cells

Chromosomal Proteins, Non-Histone

Catalytic Domain

Gene Expression Regulation, Fungal

Enzyme Inhibitors

Base Sequence

Sequence Alignment

Precipitin Tests

Binding Sites

Biocatalysis

Repressor Proteins

Fungal Proteins

Epigenesis, Genetic

Gene Expression Regulation

Protein-Arginine N-Methyltransferases

Models, Molecular

Gene Products, tat

Recombinant Proteins

Recombinant Fusion Proteins

Aminoglycosides

Methylation

Protein Kinases

RNA Polymerase II

Gene Silencing

tat Gene Products, Human Immunodeficiency Virus

Blotting, Western

Catalysis

Isoenzymes

Kinetics

Gene Deletion