Acetylserotonin O-Methyltransferase: An enzyme that catalyzes the transfer of a methyl group from S-adenosylmethionine to N-acetylserotonin to form N-acetyl-5-methoxytryptamine (MELATONIN).DNA (Cytosine-5-)-Methyltransferase: An enzyme that catalyzes the transfer of a methyl group from S-ADENOSYLMETHIONINE to the 5-position of CYTOSINE residues in DNA.Methyltransferases: A subclass of enzymes of the transferase class that catalyze the transfer of a methyl group from one compound to another. (Dorland, 28th ed) EC 2.1.1.O(6)-Methylguanine-DNA Methyltransferase: An enzyme that transfers methyl groups from O(6)-methylguanine, and other methylated moieties of DNA, to a cysteine residue in itself, thus repairing alkylated DNA in a single-step reaction. EC 2.1.1.63.Protein Methyltransferases: Enzymes that catalyze the methylation of amino acids after their incorporation into a polypeptide chain. S-Adenosyl-L-methionine acts as the methylating agent. EC 2.1.1.Methylation: Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed)Histone-Lysine N-Methyltransferase: An enzyme that catalyzes the methylation of the epsilon-amino group of lysine residues in proteins to yield epsilon mono-, di-, and trimethyllysine. EC 2.1.1.43.tRNA Methyltransferases: Enzymes that catalyze the S-adenosyl-L-methionine-dependent methylation of ribonucleotide bases within a transfer RNA molecule. EC 2.1.1.Protein-Arginine N-Methyltransferases: Enzymes that catalyze the methylation of arginine residues of proteins to yield N-mono- and N,N-dimethylarginine. This enzyme is found in many organs, primarily brain and spleen.S-Adenosylmethionine: Physiologic methyl radical donor involved in enzymatic transmethylation reactions and present in all living organisms. It possesses anti-inflammatory activity and has been used in treatment of chronic liver disease. (From Merck, 11th ed)DNA-Cytosine Methylases: Methylases that are specific for CYTOSINE residues found on DNA.Protein D-Aspartate-L-Isoaspartate Methyltransferase: A PROTEIN O-METHYLTRANSFERASE that recognizes and catalyzes the methyl esterification of ISOASPARTIC ACID and D-ASPARTIC ACID residues in peptides and proteins. It initiates the repair of proteins damaged by the spontaneous decomposition of normal L-aspartic acid and L-asparagine residues.DNA Modification Methylases: Enzymes that are part of the restriction-modification systems. They are responsible for producing a species-characteristic methylation pattern, on either adenine or cytosine residues, in a specific short base sequence in the host cell's own DNA. This methylated sequence will occur many times in the host-cell DNA and remain intact for the lifetime of the cell. Any DNA from another species which gains entry into a living cell and lacks the characteristic methylation pattern will be recognized by the restriction endonucleases of similar specificity and destroyed by cleavage. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms.Site-Specific DNA-Methyltransferase (Adenine-Specific): An enzyme responsible for producing a species-characteristic methylation pattern on adenine residues in a specific short base sequence in the host cell DNA. The enzyme catalyzes the methylation of DNA adenine in the presence of S-adenosyl-L-methionine to form DNA containing 6-methylaminopurine and S-adenosyl-L-homocysteine. EC 2.1.1.72.DNA Methylation: Addition of methyl groups to DNA. DNA methyltransferases (DNA methylases) perform this reaction using S-ADENOSYLMETHIONINE as the methyl group donor.Amido Black: A dye used to stain proteins in electrophoretic techniques. It is used interchangeably with its acid form.Neurochemistry: The study of the composition, chemical structures, and chemical reactions of the NERVOUS SYSTEM or its components.Ovarian Neoplasms: Tumors or cancer of the OVARY. These neoplasms can be benign or malignant. They are classified according to the tissue of origin, such as the surface EPITHELIUM, the stromal endocrine cells, and the totipotent GERM CELLS.Proteomics: The systematic study of the complete complement of proteins (PROTEOME) of organisms.Adenocarcinoma: A malignant epithelial tumor with a glandular organization.Lysosome-Associated Membrane Glycoproteins: Ubiquitously expressed integral membrane glycoproteins found in the LYSOSOME.Ovary: The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE.Pineal Gland: A light-sensitive neuroendocrine organ attached to the roof of the THIRD VENTRICLE of the brain. The pineal gland secretes MELATONIN, other BIOGENIC AMINES and NEUROPEPTIDES.Endocrine Glands: Ductless glands that secrete HORMONES directly into the BLOOD CIRCULATION. These hormones influence the METABOLISM and other functions of cells in the body.Endocrine Gland Neoplasms: Tumors or cancer of the ENDOCRINE GLANDS.Melatonin: A biogenic amine that is found in animals and plants. In mammals, melatonin is produced by the PINEAL GLAND. Its secretion increases in darkness and decreases during exposure to light. Melatonin is implicated in the regulation of SLEEP, mood, and REPRODUCTION. Melatonin is also an effective antioxidant.Circadian Rhythm: The regular recurrence, in cycles of about 24 hours, of biological processes or activities, such as sensitivity to drugs and stimuli, hormone secretion, sleeping, and feeding.Arylalkylamine N-Acetyltransferase: An acetyltransferase with specificity towards the amine group of aromatic alkylamines (arylalkylamines) such as SEROTONIN. This enzyme is also referred to as serotonin acetylase despite the fact that serotonin acetylation can also occur through the action of broad specificity acetyltransferases such as ARYLAMINE N-ACETYLTRANSFERASE.Fatigue: The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Catechol O-Methyltransferase: Enzyme that catalyzes the movement of a methyl group from S-adenosylmethionone to a catechol or a catecholamine.Protein O-Methyltransferase: An enzyme that catalyzes the transfer of methyl groups from S-adenosylmethionine to free carboxyl groups of a protein molecule forming methyl esters. EC 2.1.1.-.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Zebrafish: An exotic species of the family CYPRINIDAE, originally from Asia, that has been introduced in North America. They are used in embryological studies and to study the effects of certain chemicals on development.Zebrafish Proteins: Proteins obtained from the ZEBRAFISH. Many of the proteins in this species have been the subject of studies involving basic embryological development (EMBRYOLOGY).Embryo, Nonmammalian: The developmental entity of a fertilized egg (ZYGOTE) in animal species other than MAMMALS. For chickens, use CHICK EMBRYO.Clone Cells: A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)Pasteurellosis, Pneumonic: Bovine respiratory disease found in animals that have been shipped or exposed to CATTLE recently transported. The major agent responsible for the disease is MANNHEIMIA HAEMOLYTICA and less commonly, PASTEURELLA MULTOCIDA or HAEMOPHILUS SOMNUS. All three agents are normal inhabitants of the bovine nasal pharyngeal mucosa but not the LUNG. They are considered opportunistic pathogens following STRESS, PHYSIOLOGICAL and/or a viral infection. The resulting bacterial fibrinous BRONCHOPNEUMONIA is often fatal.Ships: Large vessels propelled by power or sail used for transportation on rivers, seas, oceans, or other navigable waters. Boats are smaller vessels propelled by oars, paddles, sail, or power; they may or may not have a deck.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Suprachiasmatic Nucleus: An ovoid densely packed collection of small cells of the anterior hypothalamus lying close to the midline in a shallow impression of the OPTIC CHIASM.Receptor, Melatonin, MT2: A melatonin receptor subtype primarily found expressed in the BRAIN and RETINA.Receptors, Melatonin: A family of G-protein-coupled receptors that are specific for and mediate the effects of MELATONIN. Activation of melatonin receptors has been associated with decreased intracellular CYCLIC AMP and increased hydrolysis of PHOSPHOINOSITIDES.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.5-Methoxytryptamine: Serotonin derivative proposed as potentiator for hypnotics and sedatives.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Open Reading Frames: A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The structural basis of ordered substrate binding by serotonin N-acetyltransferase: enzyme complex at 1.8 A resolution with a bisubstrate analog. (1/43)
Serotonin N-acetyltransferase, a member of the GNAT acetyltransferase superfamily, is the penultimate enzyme in the conversion of serotonin to melatonin, the circadian neurohormone. Comparison of the structures of the substrate-free enzyme and the complex with a bisubstrate analog, coenzyme A-S-acetyltryptamine, demonstrates that acetyl coenzyme A (AcCoA) binding is accompanied by a large conformational change that in turn leads to the formation of the serotonin-binding site. The structure of the complex also provides insight into how the enzyme may facilitate acetyl transfer. A water-filled channel leading from the active site to the surface provides a pathway for proton removal following amine deprotonation. Furthermore, structural and mutagenesis results indicate an important role for Tyr-168 in catalysis. (+info)Melatonin, its precursors, and synthesizing enzyme activities in the human ovary. (2/43)
The presence of melatonin (N-acetyl-5-methoxytryptamine) and its precursors, serotonin (5-hydroxytryptamine) and N-acetylserotonin, was demonstrated in extracts of human ovary using reverse-phase high-performance liquid chromatography coupled with fluorometric detection. In addition, activities of two melatonin-synthesizing enzymes, arylalkylamine N-acetyltransferase (NAT) and hydroxyindole-O-methyltransferase (HIOMT), were found in human ovary homogenates. The apparent Michaelis constants for the substrates of NAT and HIOMT in the human ovary were similar to those reported for the pineal glands of humans and other mammals. These findings strongly suggest that the human ovary, like the pineal gland, may synthesize melatonin from serotonin by the sequential action of NAT and HIOMT. (+info)Genetic variability in plasma melatonin in sheep is due to pineal weight, not to variations in enzyme activities. (3/43)
This study was conducted to determine the origin of the high variability in the mean nocturnal plasma melatonin concentration (MC) in sheep. Two extreme groups of 25 lambs each [low (L) and high (H)] were obtained by calculating their genetic value on the basis of the MC of their parents. The MC of lambs was significantly higher in the H group than in the L group (L: 189.7 +/- 24.4 vs. H: 344.1 +/- 33.0 pg/ml, P < 0.001). Within each group, 13 lambs were slaughtered during the day (D) and 12 lambs during the night (N). Pineal weight was significantly higher in the H group than in the L group (L: 83.5 +/- 6.7 vs. H: 119.1 +/- 9.2 mg, P < 0.01) but did not differ between D and N. The amount of melatonin released in vitro per milligram of pineal gland, the arylalkylamine N-acetyltransferase (AANAT) activity, the AANAT protein content, and the level of AANAT mRNA differed significantly between D and N but not with genetic group. Hydroxyindole O-methyltransferase activity did not differ significantly between D and N or between genetic groups. Therefore, the genetic difference in MC between the two groups of lambs was attributed to a difference in pineal size, not in enzymatic activity of the pinealocytes. (+info)Identification of highly elevated levels of melatonin in bone marrow: its origin and significance. (4/43)
Bone marrow is an important tissue in generation of immunocompetent and peripheral blood cells. The progenitors of hematopoietic cells in bone marrow exhibit continuous proliferation and differentiation and they are highly vulnerable to acute or chronic oxidative stress. In this investigation, highly elevated levels of the antioxidant melatonin were identified in rat bone marrow using immunocytochemistry, radioimmunoassay, high performance liquid chromatography with electrochemical detection and mass spectrometry. Night-time melatonin concentrations (expressed as pg melatonin/mg protein) in the bone marrow of rats were roughly two orders of magnitude higher than those in peripheral blood. Measurement of the activities of the two enzymes (N-acetyltransferase (NAT) and hydroxyindole-O-methoxyltransferase (HIOMT)) which synthesize melatonin from serotonin showed that bone marrow cells have measurable NAT activity, but they have very low levels of HIOMT activity (at the one time they were measured). From these studies we could not definitively determine whether melatonin was produced in bone marrow cells or elsewhere. To investigate the potential pineal origin of bone marrow melatonin, long-term (8-month) pinealectomized rats were used to ascertain if the pineal gland is the primary source of this antioxidant. The bone marrow of pinealectomized rats, however, still exhibited high levels of melatonin. These results indicate that a major portion of the bone marrow's melatonin is of extrapineal origin. Immunocytochemistry clearly showed a positive melatonin reaction intracellularly in bone marrow cells. A melatonin concentrating mechanism in these cells is suggested by these findings and this may involve a specific melatonin binding protein. Since melatonin is an endogenous free radical scavenger and an immune-enhancing agent, the high levels of melatonin in bone marrow cells may provide on-site protection to reduce oxidative damage to these highly vulnerable hematopoietic cells and may enhance the immune capacity of cells such as lymphocytes. (+info)Regulation of hydroxyindole-O-methyltransferase gene expression in Japanese quail (Coturnix coturnix japonica). (5/43)
Hydroxyindole-O-methyltrasferase (HIOMT) plays an important role as the final enzyme in the synthesis of melatonin. In this study, the expression of the HIOMT gene in Japanese quail was investigated with respect to tissue distribution and the effects of light and vitamin A deficiency. HIOMT mRNA in the pineal gland and eye had a clear daily rhythm with peak values in daytime. The testis also contained a detectable amount of HIOMT mRNA, which did not display a rhythmic change over a 24-h period. When birds were rendered vitamin A deficient through feeding with a vitamin A-free diet, the daily rhythm of the HIOMT gene almost disappeared in both the pineal gland and eye due to increases in the nighttime values. Our previous observations and these results suggest that vitamin A and a photo-signal are required to maintain the rhythmic expression of the HIOMT gene as well as the arylalkylamine N-acetyltransferase gene. (+info)Conversion of L-tryptophan to serotonin and melatonin in human melanoma cells. (6/43)
We showed in human melanoma cells tryptophan hydroxylase (TPH) and hydroxyindole methyltransferase genes expression with the sequential enzymatic activities of TPH, serotonin (Ser) N-acetyltransferase and hydroxyindole methyltransferase. The presence of the products Ser, 5OH-tryptophan, N-acetylserotonin, melatonin (Mel), 5-methoxytryptamine and 5-methoxytryptophol was documented by liquid chromatography-mass spectrometry. Thus, human melanoma cells can synthesize and metabolize Ser and Mel. (+info)Serotoninergic and melatoninergic systems are fully expressed in human skin. (7/43)
We investigated the cutaneous expression of genes and enzymes responsible for the multistep conversion of tryptophan to serotonin and further to melatonin. Samples tested were human skin, normal and pathologic (basal cell carcinoma and melanoma), cultured normal epidermal and follicular melanocytes, melanoma cell lines, normal neonatal and adult epidermal and follicular keratinocytes, squamous cell carcinoma cells, and fibroblasts from dermis and follicular papilla. The majority of the samples showed simultaneous expression of the genes for tryptophan hydroxylase, arylalkylamine N-acetyltransferase (AANAT), and hydroxyindole-O-methyltransferase (HIOMT). The products of AANAT activity were identified by RP-HPLC with fluorimetric detection in human skin and in cultured normal and malignant melanocytes and immortalized keratinocytes; HIOMT activity was detected in human skin, keratinocytes, and melanoma cells. N-acetylserotonin (NAS) was detected by RP-HPLC in human skin extracts. NAS identity was confirmed further by LC/MS in keratinocytes. In conclusion, we provide evidence that the human skin expresses intrinsic serotonin and melatonin biosynthetic pathways. (+info)Molecular cloning and nucleotide sequence of a cDNA encoding hydroxyindole O-methyltransferase from chicken pineal gland. (8/43)
Hydroxyindole O-methyltransferase (EC 2.1.1.4) is the enzyme that catalyses the synthesis of melatonin in the pineal gland and in the retina. Polyadenylated RNA from chicken pineal glands was used to prepare a cDNA library in lambda gt11. The library was screened with an antiserum directed against chicken hydroxyindole O-methyltransferase, and one cDNA clone was isolated. The fusion protein expressed by phage lysogens was identified on Western blots as a 165 kDA immunoreactive protein (beta-galactosidase, 110 kDa; hydroxyindole O-methyltransferase, 38 kDa). The fusion protein exhibited hydroxyindole O-methyltransferase activity. Its Km values for N-acetyl-5-hydroxytryptamine and S-adenosylmethionine were 5 times those of the natural enzyme. The intrinsic activity of the fusion protein was approx. 0.25% that of the natural enzyme. The cDNA consisted of 1436 nucleotides, including a 1038-nucleotide sequence encoding a full-length 346-amino-acid hydroxyindole O-methyltransferase. Comparison with bovine hydroxyindole O-methyltransferase [Ishida, Obinata & Deguchi (1987) J. Biol. Chem. 262, 2895-2899] revealed 52% identity in nucleotide sequences and 44% identity in peptide sequences. Northern-blot analysis revealed the presence of hydroxyindole O-methyltransferase mRNA transcripts in chicken pineal gland and retina, but not in the telencephalon. (+info)N-acetylserotonin is converted into melatonin by the enzyme hydroxyindole O-methyltransferase (HIOMT), also known as ... acetylserotonin O-methyltransferase (ASMT). Activity of these enzymes is high during the night and regulated by the mechanisms ... The serotonin is then acetylated by the AANAT enzyme and converted into N-acetylserotonin. ...
Hydroxyindole O-methyltransferase and S-adenosyl methionine convert N-acetylserotonin into melatonin through methylation of the ... N-acetylserotonin is methylated at the hydroxyl position by S-adenosyl methionine (SAM) to produce S-adenosyl homocysteine (SAH ... The thiol from coenzyme A serves as a good leaving group when attacked by a general base to give N-acetylserotonin. ... Donohue SJ, Roseboom PH, Illnerova H, Weller JL, Klein DC (1993). "Human hydroxyindole-O-methyltransferase: presence of LINE-1 ...
N-acetylserotonin O-methyltransferase (ASMT) to generate melatonin. The N-acetyltransferase reaction has been suggested to be ... After the transfer of the acetyl group has occurred, the products are orderly released with N-acetyl-serotonin first and CoA ... In the biosynthesis of melatonin, N-acetylserotonin is further methylated by another enzyme, ... to produce N-acetylserotonin. Based on this mechanism, it might be expected that a bisubstrate analog inhibitor, derived from ...
... thetin-homocysteine S-methyltransferase EC 2.1.1.4: acetylserotonin O-methyltransferase EC 2.1.1.5: betaine-homocysteine S- ... sterol 24-C-methyltransferase EC 2.1.1.42: luteolin O-methyltransferase EC 2.1.1.43: histone-lysine N-methyltransferase EC 2.1. ... methyltransferase EC 2.1.1.6: catechol O-methyltransferase EC 2.1.1.7: nicotinate N-methyltransferase EC 2.1.1.8: histamine N- ... phenol O-methyltransferase EC 2.1.1.26: iodophenol O-methyltransferase EC 2.1.1.27: tyramine N-methyltransferase EC 2.1.1.28: ...
... acetylserotonin n-methyltransferase MeSH D08.811.913.555.500.175 --- betaine-homocysteine S-methyltransferase MeSH D08.811. ... histone-lysine n-methyltransferase MeSH D08.811.913.555.500.800.650 --- o-6-methylguanine-DNA methyltransferase MeSH D08.811. ... protein o-methyltransferase MeSH D08.811.913.555.500.800.800.700 --- protein d-aspartate-l-isoaspartate methyltransferase MeSH ... site-specific dna-methyltransferase (adenine-specific) MeSH D08.811.913.555.500.350.850 --- site-specific dna methyltransferase ...
As of the year 2000, a number of genes were known to be Y-linked, including: ASMTY (acetylserotonin methyltransferase), TSPY ( ...
... and is converted to melatonin by acetylserotonin O-methyltransferase (ASMT). NAS is able to penetrate the blood-brain barrier, ... N-Acetylserotonin (NAS), also known as normelatonin, is a naturally occurring chemical precursor and intermediate in the ... 1999). "N-acetylserotonin is a better extra- and intracellular antioxidant than melatonin". FEBS Lett. 449 (2-3): 206-10. doi: ... February 2010). "N-acetylserotonin activates TrkB receptor in a circadian rhythm". Proceedings of the National Academy of ...
Examples are : Acetylserotonin O-methyltransferase Apigenin 4'-O-methyltransferase Caffeate O-methyltransferase Caffeoyl-CoA O- ... O-methyltransferase Kaempferol 4'-O-methyltransferase Licodione 2'-O-methyltransferase Loganate O-methyltransferase Luteolin O- ... O-methyltransferase Phenol O-methyltransferase Polysaccharide O-methyltransferase Protein-glutamate O-methyltransferase Protein ... methyltransferase Catechol O-methyltransferase Chlorophenol O-methyltransferase Columbamine O-methyltransferase ...
... (ASMT), Acetylserotonin N-methyltransferase, Acetylserotonin methyltransferase (Y ... Acetylserotonin O-methyltransferase The second reaction (Figure 4) catalyzed by N-Acetylserotonin O-methyltransferase in the ... Synonyms of N- Acetylserotonin O-methyltransferase are Hydroxyindole O-methyltransferase (HIOMT), ... suggests that Hydroxyindole O-methyltransferase (synonym for N- Acetylserotonin O-methyltransferase) plays a role in the human ...
... is an enzyme that in humans is encoded by the ASMTL gene. GRCh38: Ensembl ... "Entrez Gene: ASMTL acetylserotonin O-methyltransferase-like". Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to ...
... is known to act as a histamine N-methyltransferase inhibitor. This explains how harmaline elicits its wakefulness- ... Harmaline forces the anabolic metabolism of serotonin into normelatonin or n-acetylserotonin, and then to melatonin, the body's ... Cumming, P; Vincent SR (September 1992). "Inhibition of histamine-N-methyltransferase (HNMT) by fragments of 9-amino-1,2,3,4- ...
Acetylserotonin O-methyltransferase. arginine→NO. *Nitric oxide synthase (NOS1, NOS2, NOS3). choline→Acetylcholine. ...
... which can be further modified by the enzyme phenylethanol N-methyltransferase to obtain epinephrine.[11] Since L-DOPA is the ...
5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase. *Catechol-O-methyl transferase. Homocysteine. *Betaine ... Information about DNA methyltransferases and DNA methylation at epigeneticstation.com. *Data for a DNA methyltransferase (DNMT ... DNMT1 is the most abundant DNA methyltransferase in mammalian cells, and considered to be the key maintenance methyltransferase ... methyltransferases. Classification of all DNA methyltransferases". Gene. 157 (1-2): 3-11. doi:10.1016/0378-1119(94)00783-O. ...
5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase. *Catechol-O-methyl transferase. Homocysteine. *Betaine ... Thiopurine methyltransferase or thiopurine S-methyltransferase (TPMT) is an enzyme that in humans is encoded by the TPMT gene. ... methyltransferase activity. • thiopurine S-methyltransferase activity. • S-adenosyl-L-methionine binding. Cellular component. • ... evidence for catalysis by human liver thiol methyltransferase and thiopurine methyltransferase". J. Pharmacol. Exp. Ther. 266 ( ...
ASMT(英语:Acetylserotonin O-methyltransferase)抑製劑. 异化作用 ...
Hydroxyindole O-methyltransferase and S-adenosyl methionine convert N-acetylserotonin into melatonin through methylation of the ... The thiol from coenzyme A serves as a good leaving group when attacked by a general base to give N-acetylserotonin.[61] ... N-acetylserotonin is methylated at the hydroxyl position by S-adenosyl methionine (SAM) to produce S-adenosyl homocysteine (SAH ... Serotonin is itself an important neurotransmitter, but is also converted into N-acetylserotonin by serotonin N- ...
5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase. *Catechol-O-methyl transferase. Homocysteine. *Betaine ...
... for example by catechol-O-methyl transferase, and is therefore no longer able to act as an electron acceptor. Many polyphenols ...
Hydroxyindole O-methyltransferase and S-adenosyl methionine convert N-acetylserotonin into melatonin through methylation of the ... The thiol from coenzyme A serves as a good leaving group when attacked by a general base to give N-acetylserotonin.[58] ... N-acetylserotonin is methylated at the hydroxyl position by S-adenosyl methionine (SAM) to produce S-adenosyl homocysteine (SAH ... Serotonin is itself an important neurotransmitter, but is also converted into N-acetylserotonin by serotonin N- ...
N-acetylserotonin is converted into melatonin by the enzyme hydroxyindole O-methyltransferase (HIOMT), also known as ... acetylserotonin O-methyltransferase (ASMT). Activity of these enzymes is high during the night and regulated by the mechanisms ... The serotonin is then acetylated by the AANAT enzyme and converted into N-acetylserotonin. ...
Acetylserotonin O-methyltransferase (ASMT), Acetylserotonin N-methyltransferase, Acetylserotonin methyltransferase (Y ... Acetylserotonin O-methyltransferase The second reaction (Figure 4) catalyzed by N-Acetylserotonin O-methyltransferase in the ... Synonyms of N- Acetylserotonin O-methyltransferase are Hydroxyindole O-methyltransferase (HIOMT), ... suggests that Hydroxyindole O-methyltransferase (synonym for N- Acetylserotonin O-methyltransferase) plays a role in the human ...
N-acetylserotonin O-methyltransferase-like protein is an enzyme that in humans is encoded by the ASMTL gene. GRCh38: Ensembl ... "Entrez Gene: ASMTL acetylserotonin O-methyltransferase-like". Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to ...
Compare acetylserotonin O-methyltransferase like ELISA Kits from leading suppliers on Biocompare. View specifications, prices, ... acetylserotonin O-methyltransferase like ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established ... Your search returned 23 acetylserotonin O-methyltransferase like ELISA ELISA Kit across 2 suppliers. ...
GoPubMed lists recent and important papers and reviews for acetylserotonin O-methyltransferase(hydroxyindole-O- ... The N-acetylserotonin O-methyltransferase (ASMT) gene encodes the enzyme that catalyzes the conversion of N-acetylserotonin to ... Cloning and functional characterization of the Arabidopsis N-acetylserotonin O-methyltransferase responsible for melatonin ... and N-acetylserotonin-O-methyltransferase (ASMT) was analyzed by RT-PCR, q-PCR and Western-blot in ram testes, epididymis, and ...
Recombinant Protein and Acetylserotonin O-methyltransferase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and ... Acetylserotonin O-methyltransferase 1. Acetylserotonin O-methyltransferase 1 ELISA Kit. Acetylserotonin O-methyltransferase 1 ... Acetylserotonin O-methyltransferase 2. Acetylserotonin O-methyltransferase 2 ELISA Kit. Acetylserotonin O-methyltransferase 2 ... Acetylserotonin O-methyltransferase 3. Acetylserotonin O-methyltransferase 3 ELISA Kit. Acetylserotonin O-methyltransferase 3 ...
... while the C-terminus is similar to N-acetylserotonin O-methyltransferase. This gene is located in the pseudoautosomal region 1 ... The presence of the putative catalytic domain of S-adenosyl-L-methionine binding argues for a methyltransferase activity. The ...
Variants of acetylserotonin o-methyltransferase and uses thereof Luo, Hao Patent no.: WO2017202897, Date: 2017-11-30 ... Method of improving methyltransferase activity Luo, Hao ; Hansen, Anne Sofie Lærke Patent no.: WO2018037098, Date: 2018-03-01 ...
The enzymes AANAT and acetylserotonin O-methyltransferase (ASMT) catalyze the conversion of serotonin to melatonin. Nocturnal ... J. Shen, K. Ghai, P. Sompol et al., "N-acetyl serotonin derivatives as potent neuroprotectants for retinas," Proceedings of the ... M. Bernard, V. Dinet, and P. Voisin, "Transcriptional regulation of the chicken hydroxyindole-O-methyltransferase gene by the ... D. C. Klein and R. Y. Moore, "Pineal N-acetyltransferase and hydroxyindole-O-methyltransferase: control by the ...
acetylserotonin O-methyltransferase , hydroxyindole O-methyltransferase , hydroxyindole-O-methyltransferase , acetylserotonin N ... methyltransferase , acetylserotonin methyltransferase (Y chromosome) , HIOMT , acetylserotonin methyltransferase , ... Acetylserotonin O-Methyltransferase (ASMT) ELISA Kits. ASMT belongs to the methyltransferase superfamily, and is located in the ... Cow (Bovine) Acetylserotonin O-Methyltransferase (ASMT) interaction partners * The study revealed that ASMT and MTNR1A (show ...
Acetylserotonin O-methyltransferase-like. 1.62. 3.15. 1.62. 210147571. Acidic (leucine-rich) nuclear phosphoprotein 32 family, ...
Subsequently that compound is catalyzed to melatonin by acetylserotonin O-methyltransferase (ASMT). The rise in circulating ... catalyzes the conversion of serotonin to N-acetylserotonin. ...
Thetin-homocysteine S-methyltransferase Pages 12-14 * Acetylserotonin O-methyltransferase Pages 15-20 ...
HUMAN 402500 Acetylserotonin O-methyltransferase (EC 2.1.1.4) (Hydroxyindole O-methyltransferase) (HIOMT) ASMTL Xp22.3 O95671 ... N-acetylserotonin O-methyltransferase-like protein (EC 2.1.1.-) (ASMTL)] ATG4A Xq22.1-q22.3 Q8WYN0 ATG4A_HUMAN 300663 Cysteine ... methyltransferase (EC 2.1.1.205) (2-O-ribose RNA methyltransferase TRM7 homolog) (Protein ftsJ homolog 1) [JM23] FUNDC1 Xp11.4 ... Histone H3-K9 methyltransferase 1) (H3-K9-HMTase 1) (Lysine N-methyltransferase 1A) (Position-effect variegation 3-9 homolog) ( ...
... isoflavone-7-O-methyltransferase (EC:2.1.1.150), inositol 4-methyltransferase (EC:2.1.1.129) and acetylserotonin O- ... O-methyltransferase COMT-type (IPR016461). Short name: O-MeTrfase_COMT Family relationships *O-methyltransferase COMT-type ( ... Molecular cloning of a plant N-acetylserotonin methyltransferase and its expression characteristics in rice.. J. Pineal Res. 50 ... This entry represents a family of O-methyltransferases (O-MTases) that includes caffeic acid O-methyltransferase (COMT) (EC:2.1 ...
Acetylserotonin O-methyltransferase. Aliases:. Not Available. RefSeq:. Not Available. Ensembl:. ENSCAFG00000024670 Entrez:. 0 ...
Deficiency of acetylserotonin methyltransferase. *Deficiency of acid-aminoacid ligase. *Deficiency of acid-ammonia ligase ...
Acetylserotonin o-methyltransferase-like. Functionally important candidate genes found in All-Summer, VMN-summer, and Peak-SNV ...
Acetylserotonin O-Methyltransferase1. *Adrenocorticotropic Hormone1. *Catecholamines1. *Circadian Rhythm1 ...
Next-day shipping cDNA ORF clones derived from asmtl acetylserotonin O-methyltransferase-like available at GenScript, starting ... Danio rerio acetylserotonin O-methyltransferase-like (asmtl), mRNA.. pcDNA3.1+/C-(K)DYK or customized vector. 7-9. $342.30. $ ... N-acetylserotonin O-methyltransferase-like protein. Comment. Comment: PROVISIONAL REFSEQ: This record has not yet been subject ... asmtl ( NM_213511.1 ) cDNA ORF clone, Danio rerio(zebrafish) -, NP_998676.1 Danio rerio acetylserotonin O-methyltransferase- ...
Julius Axelrod found that the mechanism behind the breakdown of catecholamine was mediated by catechol-O-methyltransferase - ...
A Comparative Genomic and Transcriptomic Survey Provides Novel Insights into N-Acetylserotonin Methyltransferase (ASMT) in Fish ...
... and N-acetylserotonin O-methyltransferase (ASMT)] (Kang et al., 2007a,b, 2011, 2013; Okazaki et al., 2009; Fujiwara et al., ... Recent studies found that N-acetylserotonin can also be synthesized via caffeic acid O-methyltransferase (COMT) in Arabidopsis ... Caffeic acid O-methyltransferase is involved in the synthesis of melatonin by methylating N-acetylserotonin in Arabidopsis. J. ... Molecular cloning of a plant N-acetylserotonin methyltransferase and its expression characteristics in rice. J. Pineal Res. 50 ...
Molecular cloning of a plant N-acetylserotonin methyltransferase and its expression characteristics in rice. J. Pineal Res. 50 ... temperatures and under dark conditions due to increased serotonin N-acetyltransferase and N-acetylserotonin methyltransferase ... and N-aceylserotonin O-methyltransferase (ASMT) (Arnao and Hernández-Ruiz, 2014, 2015; Zuo et al., 2014). Thereafter, melatonin ... N-aceylserotonin methyltransferase; AXR3, Auxin Resistant 3; BL, indole-3-acetic acid; DAT, days after transplanting; GA, ...
OTTHUMP00000022825,acetylserotonin N-methyltransferase-like. *Related Product. *H00008623-B01P. * ASMTL purified MaxPab mouse ...
- The most commonly used synonym is Hydroxyindole O-methyltransferase (HIOMT). (wikipedia.org)
- This finding, in conjunction with research on alternative splicing of the HOIMT hnRNA, suggests that Hydroxyindole O-methyltransferase (synonym for N- Acetylserotonin O-methyltransferase) plays a role in the human immune system, in addition to its endocrine and nervous system functions. (wikipedia.org)
- The biosynthetic steps of melatonin with two major rate limiting enzymes, arylalkylamine-N-acetyltransferase (AA-NAT) and hydroxyindole-O-methyltransferase (HIOMT), transforming tryptophan to melatonin, originally identified in pinealocytes have been also detected in entero-endocrine (EE) cells of GIT wall, where this indole may act via endocrine, paracrine and/or luminal pathway through G-protein coupled receptors. (gopubmed.org)
- Also known as Acetylserotonin O-methyltransferase (Hydroxyindole O-methyltransferase). (mybiosource.com)
- This is methylated by the enzyme hydroxyindole-o-methyltransferase to produce melatonin. (digitaljournal.com)
- In the rat, this stress caused a concurrent increase in pineal serotonin N-acetyltransferase (NAT) activity and melatonin content with no observable change in hydroxyindole-O-methyltransferase (HIOMT) activity. (elsevier.com)
- Structural analysis of the human hydroxyindole-O-methyltransferase gene. (wikipathways.org)
- 5-MTP is synthesized in human fibroblasts via two enzymatic steps: tryptophan hydroxylase-1 (TPH-1) which catalyzes conversion of L-tryptophan to 5-hydroxytryptophan (5-HTP) and hydroxyindole O-methyltransferase (HIOMT) which catalyzes transfer of methyl group from a methyl donor to 5-HTP to form 5-MTP. (oatext.com)
- By the action of hydroxyindole-O-methyltransferase (HIOMT), N-acetylserotonin is converted to melatonin. (lymeneteurope.org)
- 5-HTP is converted to serotonin by the aromatic amino acid decarboxylase, and serotonin is subsequently converted into N-acetylserotonin (NAS) by the enzyme arylalkylamine N-acetyltransferase. (hindawi.com)
- Additionally, arylalkylamine N-acetyltransferase ( AANAT ) polymorphisms have been reported associated with depression, perhaps through their influence upon N-acetylserotonin or melatonin synthesis. (biomedcentral.com)
- Cloning and functional characterization of the Arabidopsis N- acetylserotonin O- methyltransferase responsible for melatonin synthesis. (gopubmed.org)
- Results: Expression of melatonin in adult anemones showed peak expression at the end of light period (zeitgeber time (ZT) = 12) when cultured under diel conditions, coinciding with expression of genes and enzyme activity for members of the melatonin synthesis pathway (tryptophan hydroxylase and hydroxyindol-O-methyltransferase), which also showed rhythmic expression. (ufl.edu)
- Serotonin has many fates in this pathway, and N- Acetylserotonin O-methyltransferase catalyzes reactions in two of these fates. (wikipedia.org)
- In the generally rate-limiting step of melatonin biosynthesis, an enzyme called serotonin N-acetyltransferase (AANAT) catalyzes the conversion of serotonin to N-acetylserotonin. (britannica.com)
- Serotonin is then converted by the enzyme N-acetyl transferase to the precursor of melatonin, N-acetylserotonin. (digitaljournal.com)
- In this study, we demonstrate microbial production of melatonin and related compounds, such as serotonin and N-acetylserotonin. (dtu.dk)
- We generated Saccharomyces cerevisiae strains that comprise heterologous genes encoding one or more variants of an L-tryptophan hydroxylase, a 5-hydroxy-L-tryptophan decarboxylase, a serotonin acetyltransferase, an acetylserotonin O-methyltransferase, and means for providing the cofactor tetrahydrobiopterin via heterologous biosynthesis and recycling pathways. (dtu.dk)
- Only in darkness, the key enzyme, aralkylamine N-acetyltransferase (AANAT) is activated and converts serotonin to N-acetyl serotonin, which is ultimately converted to melatonin by the final enzyme, acetylserotonin O-methyltransferase. (worldheritage.org)
- This entry represents a family of O-methyltransferases (O-MTases) that includes caffeic acid O-methyltransferase (COMT) ( EC:2.1.1.68 ), isoflavone-7-O-methyltransferase ( EC:2.1.1.150 ), inositol 4-methyltransferase ( EC:2.1.1.129 ) and acetylserotonin O-methyltransferase [ PMID: 21210840 ]. (ebi.ac.uk)
- A caffeic acid/5-hydroxyferulic acid O-methyltransferase. (mybiosource.com)
- This gene belongs to the methyltransferase superfamily, and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes. (gopubmed.org)
- Molecular cloning of cDNA and chromosomal assignment of the gene for human phenylethanolamine N-methyltransferase, the enzyme for epinephrine biosynthesis. (wikipathways.org)
- The presence of the putative catalytic domain of S-adenosyl-L-methionine binding argues for a methyltransferase activity. (nih.gov)
- Dopamine may be converted into norepinephrine by the enzyme dopamine β-hydroxylase , which can be further modified by the enzyme phenylethanol N-methyltransferase to obtain epinephrine. (wikidoc.org)
- Catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine). (mybiosource.com)
- More than 230 families of methyltransferases have been described so far, of which more than 220 use SAM as the methyl donor. (ebi.ac.uk)
- Universal catalytic domain structure of AdoMet-dependent methyltransferases. (ebi.ac.uk)
- Also known as Flavone 3'-O-methyltransferase 1 (AtOMT1) (Acetylserotonin O-methyltransferase OMT1) (Caffeate O-methyltransferase 1) (Quercetin 3'-O-methyltransferase 1). (mybiosource.com)
- O-methyltransferase that is highly active towards quercetin and myricetin. (mybiosource.com)
- Even within the structurally conserved family of Class I methyltransferases, a wide variety of mechanisms have evolved to activate the catalytic nucleophile, dependent on the polarizability of the target atom [ PMID: 12826405 ]. (ebi.ac.uk)
- N-Acetylserotonin O-methyltransferase can be classified under three types of enzyme functional groups: transferases, one-carbon group transferers, and methyltransferases. (wikipedia.org)
- Molecular cloning of a plant N-acetylserotonin methyltransferase and its expression characteristics in rice. (ebi.ac.uk)