Acetylglucosaminidase: A beta-N-Acetylhexosaminidase that catalyzes the hydrolysis of terminal, non-reducing 2-acetamido-2-deoxy-beta-glucose residues in chitobiose and higher analogs as well as in glycoproteins. Has been used widely in structural studies on bacterial cell walls and in the study of diseases such as MUCOLIPIDOSIS and various inflammatory disorders of muscle and connective tissue.Genomic Structural Variation: Contiguous large-scale (1000-400,000 basepairs) differences in the genomic DNA between individuals, due to SEQUENCE DELETION; SEQUENCE INSERTION; or SEQUENCE INVERSION.Glycosylation: The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.Glycoconjugates: Carbohydrates covalently linked to a nonsugar moiety (lipids or proteins). The major glycoconjugates are glycoproteins, glycopeptides, peptidoglycans, glycolipids, and lipopolysaccharides. (From Biochemical Nomenclature and Related Documents, 2d ed; From Principles of Biochemistry, 2d ed)Hexosaminidases: Enzymes that catalyze the hydrolysis of N-acylhexosamine residues in N-acylhexosamides. Hexosaminidases also act on GLUCOSIDES; GALACTOSIDES; and several OLIGOSACCHARIDES.Endo-1,4-beta Xylanases: Enzymes which catalyze the endohydrolysis of 1,4-beta-D-xylosidic linkages in XYLANS.N-Acetylmuramoyl-L-alanine Amidase: An autolytic enzyme bound to the surface of bacterial cell walls. It catalyzes the hydrolysis of the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell wall glycopeptides, particularly peptidoglycan. EC 3.5.1.28.Mucopolysaccharidosis III: Mucopolysaccharidosis characterized by heparitin sulfate in the urine, progressive mental retardation, mild dwarfism, and other skeletal disorders. There are four clinically indistinguishable but biochemically distinct forms, each due to a deficiency of a different enzyme.Mucopolysaccharidoses: Group of lysosomal storage diseases each caused by an inherited deficiency of an enzyme involved in the degradation of glycosaminoglycans (mucopolysaccharides). The diseases are progressive and often display a wide spectrum of clinical severity within one enzyme deficiency.Phosphoric Diester Hydrolases: A class of enzymes that catalyze the hydrolysis of one of the two ester bonds in a phosphodiester compound. EC 3.1.4.Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase: A group of related enzymes responsible for the endohydrolysis of the di-N-acetylchitobiosyl unit in high-mannose-content glycopeptides and GLYCOPROTEINS.Immunoelectrophoresis: A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.beta-N-Acetyl-Galactosaminidase: A hexosiminidase that specifically hydrolyzes terminal non-reducing N-acetyl-D-galactosamine residues in N-acetyl-beta-D-galactosaminides.Bacillus subtilis: A species of gram-positive bacteria that is a common soil and water saprophyte.Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor: Enzymes that catalyze the joining of glutamine-derived ammonia and another molecule. The linkage is in the form of a carbon-nitrogen bond. EC 6.3.5.Hydrolysis: The process of cleaving a chemical compound by the addition of a molecule of water.Receptors, Neuropeptide Y: Cell surface proteins that bind neuropeptide Y with high affinity and trigger intracellular changes which influence the behavior of cells.Rhabditoidea: A superfamily of nematodes of the order RHABDITIDA. Characteristics include an open tube stoma and an excretory system with lateral canals.Corynebacterium glutamicum: A species of gram-positive, asporogenous, non-pathogenic, soil bacteria that produces GLUTAMIC ACID.Corynebacterium: A genus of asporogenous bacteria that is widely distributed in nature. Its organisms appear as straight to slightly curved rods and are known to be human and animal parasites and pathogens.Kinetics: The rate dynamics in chemical or physical systems.Acetylglucosamine: The N-acetyl derivative of glucosamine.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Chromosome Mapping: Any method used for determining the location of and relative distances between genes on a chromosome.Chromosomes: In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.MedlinePlus: NATIONAL LIBRARY OF MEDICINE service for health professionals and consumers. It links extensive information from the National Institutes of Health and other reviewed sources of information on specific diseases and conditions.Molecular Sequence Annotation: The addition of descriptive information about the function or structure of a molecular sequence to its MOLECULAR SEQUENCE DATA record.Chromosome Banding: Staining of bands, or chromosome segments, allowing the precise identification of individual chromosomes or parts of chromosomes. Applications include the determination of chromosome rearrangements in malformation syndromes and cancer, the chemistry of chromosome segments, chromosome changes during evolution, and, in conjunction with cell hybridization studies, chromosome mapping.Clostridium perfringens: The most common etiologic agent of GAS GANGRENE. It is differentiable into several distinct types based on the distribution of twelve different toxins.Clostridium Infections: Infections with bacteria of the genus CLOSTRIDIUM.N-Acetylglucosaminyltransferases: Enzymes that catalyze the transfer of N-acetylglucosamine from a nucleoside diphosphate N-acetylglucosamine to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.Clostridium: A genus of motile or nonmotile gram-positive bacteria of the family Clostridiaceae. Many species have been identified with some being pathogenic. They occur in water, soil, and in the intestinal tract of humans and lower animals.Enteritis: Inflammation of any segment of the SMALL INTESTINE.Chemokine CCL3: A CC chemokine with specificity for CCR1 RECEPTORS and CCR5 RECEPTORS. It is a chemoattractant for NK CELLS; MONOCYTES; and a variety of other immune cells. This chemokine is encoded by multiple genes.Receptors, CXCR: Chemokine receptors that are specific for CXC CHEMOKINES.Rhipicephalus sanguineus: A species of tick (TICKS) in the family IXODIDAE, distributed throughout the world but abundant in southern Europe. It will feed on a wide variety of MAMMALS, but DOGS are its preferred host. It transmits a large number of diseases including BABESIOSIS; THEILERIASIS; EHRLICHIOSIS; and MEDITERRANEAN SPOTTED FEVER.Leukocyte Adherence Inhibition Test: Test for cell-mediated antitumor immunity and related serum blocking factors based on the finding that leukocytes from cancer patients, but not from controls, when mixed in vitro with antigenic extracts of tumors of the same histological type, undergo a diminution in their normal adherence to glass surfaces. Sera from tumor-bearing patients block the LAI reaction of their own leukocytes or those of other patients with the same type of tumor.Leukocytes: White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).Graft vs Host Disease: The clinical entity characterized by anorexia, diarrhea, loss of hair, leukopenia, thrombocytopenia, growth retardation, and eventual death brought about by the GRAFT VS HOST REACTION.Chemokine CCL4: A CC chemokine with specificity for CCR5 RECEPTORS. It is a chemoattractant for NK CELLS; MONOCYTES and a variety of other immune cells. This chemokine is encoded by multiple genes.Histone Acetyltransferases: Enzymes that catalyze acyl group transfer from ACETYL-CoA to HISTONES forming CoA and acetyl-histones.Acetyltransferases: Enzymes catalyzing the transfer of an acetyl group, usually from acetyl coenzyme A, to another compound. EC 2.3.1.Rodenticides: Substances used to destroy or inhibit the action of rats, mice, or other rodents.p300-CBP Transcription Factors: A family of histone acetyltransferases that is structurally-related to CREB-BINDING PROTEIN and to E1A-ASSOCIATED P300 PROTEIN. They function as transcriptional coactivators by bridging between DNA-binding TRANSCRIPTION FACTORS and the basal transcription machinery. They also modify transcription factors and CHROMATIN through ACETYLATION.beta-N-Acetylhexosaminidases: A hexosaminidase specific for non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. It acts on GLUCOSIDES; GALACTOSIDES; and several OLIGOSACCHARIDES. Two specific mammalian isoenzymes of beta-N-acetylhexoaminidase are referred to as HEXOSAMINIDASE A and HEXOSAMINIDASE B. Deficiency of the type A isoenzyme causes TAY-SACHS DISEASE, while deficiency of both A and B isozymes causes SANDHOFF DISEASE. The enzyme has also been used as a tumor marker to distinguish between malignant and benign disease.Rodent Control: The reduction or regulation of the population of noxious, destructive, or dangerous rodents through chemical, biological, or other means.PolysaccharidesStereoisomerism: The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Animal Husbandry: The science of breeding, feeding and care of domestic animals; includes housing and nutrition.Glycosyltransferases: Enzymes that catalyze the transfer of glycosyl groups to an acceptor. Most often another carbohydrate molecule acts as an acceptor, but inorganic phosphate can also act as an acceptor, such as in the case of PHOSPHORYLASES. Some of the enzymes in this group also catalyze hydrolysis, which can be regarded as transfer of a glycosyl group from the donor to water. Subclasses include the HEXOSYLTRANSFERASES; PENTOSYLTRANSFERASES; SIALYLTRANSFERASES; and those transferring other glycosyl groups. EC 2.4.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Carbohydrate Sequence: The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.

An improved method for the structural profiling of keratan sulfates: analysis of keratan sulfates from brain and ovarian tumors. (1/982)

A previously developed method for the structural fingerprinting of keratan sulfates (Brown et al., Glycobiology, 5, 311-317, 1995) has been adapted for use with oligosaccharides fluorescently labeled with 2-aminobenzoic acid following keratanase II digestion. The oligosaccharides are separated by high-pH anion-exchange chromatography on a Dionex AS4A-SC column. This methodology permits quantitative analysis of labeled oligosaccharides which can be detected at the sub-nanogram ( approximately 100 fmol) level. Satisfactory calibration of this method can be achieved using commercial keratan sulfate standards. Keratan sulfates from porcine brain phosphocan and human ovarian tumors have been examined using this methodology, and their structural features are discussed.  (+info)

Effect of fasting on temporal variation in the nephrotoxicity of amphotericin B in rats. (2/982)

Evidence for temporal variation in the nephrotoxicity of amphotericin B was recently reported in experimental animals. The role of food in these variations was determined by studying the effect of a short fasting period on the temporal variation in the renal toxicity of amphotericin B. Twenty-eight normally fed and 28 fasted female Sprague-Dawley rats were used. Food was available ad libitum to the fed rats, while the fasted animals were fasted 12 h before and 24 h after amphotericin B injection to minimize stress for the animals. Water was available ad libitum to both groups of rats, which were maintained on a 14-h light, 10-h dark regimen (light on at 0600 h). Renal toxicity was determined by comparing the levels of excretion of renal enzyme and the serum creatinine and blood urea nitrogen (BUN) levels at the time of the maximal (0700 h) or the minimal (1900 h) nephrotoxicity after the intraperitoneal administration of a single dose of dextrose (5%; control group) or amphotericin B (50 mg/kg of body weight; treated group) to the rats. The nephrotoxicities obtained after amphotericin B administration at both times of day were compared to the nephrotoxicities observed for time-matched controls. In fed animals, the 24-h urinary excretion of N-acetyl-beta-D-glucosaminidase and beta-galactosidase was significantly higher when amphotericin B was injected at 0700 and 1900 h. The excretion of these two enzymes was reduced significantly (P < 0.05) in fasting rats, and this effect was larger at 0700 h (P < 0.05) than at 1900 h. The serum creatinine level was also significantly higher (P < 0.05) in fed animals treated at 0700 h than in fed animals treated at 1900 h. Fasting reduced significantly (P < 0.05) the increase in the serum creatinine level, and this effect was larger in the animals treated at 0700 h. Similar data were obtained for BUN levels. Amphotericin B accumulation was significantly higher (P < 0.05) in the renal cortexes of fed rats than in those of fasted animals, but there was no difference according to the time of injection. These results demonstrated that fasting reduces the nephrotoxicity of amphotericin B and that food availability is of crucial importance in the temporal variation in the renal toxicity of amphotericin B in rats.  (+info)

Endometrial lysosomal enzyme activity in normal cycling endometrium. (3/982)

The objective of this study was to evaluate the possible role of four lysosomal enzymes in endometrial function and remodelling during the normal menstrual cycle by fluorimetric measurement (acid phosphatase, N-acetyl-beta-D-glucosaminidase, alpha-L-fucosidase and alpha-D-mannosidase). A prospective study was conducted of 45 endometrial biopsies obtained from women with normal menstrual cycles. Activity of all four enzymes was identified in human endometrium. Activity of acid phosphatase and N-acetyl-beta-D-glucosaminidase was relatively high, whilst that of alpha-L-fucosidase and alpha-D-mannosidase was low. There was no significant change in the activity of any of the four enzymes from the proliferative to the secretory phase of the cycle. This study suggests that the activity of these enzymes remains constant throughout a major portion of the normal cycle.  (+info)

Immediate and early renal function after living donor transplantation. (4/982)

BACKGROUND: In order to assess the immediate renal function after living donor transplantation, renal function was compared in eight renal allograft recipients and their living related kidney donors during the first 24 h after transplantation. METHODS: Substantial and comparable intraoperative volume loading with Ringer's acetate and mannitol was performed together with the administration of frusemide. Glomerular filtration rate (GFR) and effective renal plasma flow (ERPF) were estimated by the clearances of inulin and p-aminohippurane, respectively. Tubular reabsorptive function and injury were estimated from the clearance of lithium, the fractional excretion of sodium and the urinary excretion of N-acetyl-beta-glucosaminidase. RESULTS: One hour after completion of surgery, GFR (54 +/- 7 ml/min) and ERPF (294 +/- 35 ml/min) were only 30% lower in the grafts than in the remaining donor kidneys, increasing to similar levels within 3 h. Only minor tubular dysfunction and injury were revealed in the grafted kidneys, and these tended to normalize within 24 h. CONCLUSIONS: By the present transplantation procedure comprising short ischaemia time and substantial volume expansion combined with mannitol and frusemide administration, kidneys from living donors regain nearly normal function within a few hours after transplantation.  (+info)

Active site characterization of the exo-N-acetyl-beta-D- glucosaminidase from thermotolerant Bacillus sp. NCIM 5120: involvement of tryptophan, histidine and carboxylate residues in catalytic activity. (5/982)

The exo-N-acetyl-beta-d-glucosaminidase (EC 3.2.1.30) from thermotolerant Bacillus sp. NCIM 5120 is a homotetramer with a molecular mass of 240000 kDa. Chemical modification studies on the purified exo-N-acetyl-beta-d-glucosaminidase revealed the involvement of a single tryptophan, histidine and carboxylate, per monomer, in the catalytic activity of the enzyme. Spectral analysis and maintenance of total enzyme activities indicated that N-acetylglucosamine (competitive inhibitor) and p-nitrophenyl-N-acetyl-beta-d-glucosaminide (substrate) prevented the modification of a single essential tryptophan, histidine and carboxylate residue. Kinetic parameters of partially inactivated enzyme (by NBS/HNBB) showed the involvement of tryptophan in substrate binding while that of histidine (by photooxidation/DEPC) and carboxylate (by EDAC/WRK) in catalysis. The Bacillus sp. NCIM 5120 exo-N-acetyl-beta-d-glucosaminidase deviates from the reported N-acetyl-beta-d-glucosaminidases and beta-hexosaminidases that utilize anchimeric assistance in their hydrolytic mechanism.  (+info)

Sanfilippo type B syndrome (mucopolysaccharidosis III B): allelic heterogeneity corresponds to the wide spectrum of clinical phenotypes. (6/982)

Sanfilippo B syndrome (mucopolysaccharidosis IIIB, MPS IIIB) is caused by a deficiency of alpha-N-acetylglucosaminidase, a lysosomal enzyme involved in the degradation of heparan sulphate. Accumulation of the substrate in lysosomes leads to degeneration of the central nervous system with progressive dementia often combined with hyperactivity and aggressive behaviour. Age of onset and rate of progression vary considerably, whilst diagnosis is often delayed due to the absence of the pronounced skeletal changes observed in other mucopolysaccharidoses. Cloning of the gene and cDNA encoding alpha-N-acetylglucosaminidase enabled a study of the molecular basis of this syndrome. We were able to identify 31 mutations, 25 of them novel, and two polymorphisms in the 40 patients mostly of Australasian and Dutch origin included in this study. The observed allellic heterogeneity reflects the wide spectrum of clinical phenotypes reported for MPS IIIB patients. The majority of changes are missense mutations; also four nonsense and nine frameshift mutations caused by insertions or deletions were identified. Only five mutations were found in more than one patient and the observed frequencies are well below those observed for the common mutations in MPS IIIA. R643C and R297X each account for around 20% of MPS IIIB alleles in the Dutch patient group, whilst R297X, P521L, R565W and R626X each have a frequency of about 6% in Australasian patients. R643C seems to be a Dutch MPS IIIB allele and clearly confers the attenuated phenotype. One region of the gene shows a higher concentration of mutations, probably reflecting the instability of this area which contains a direct repeat. Several arginine residues seem to be 'hot-spots' for mutations, being affected by two or three individual base pair exchanges.  (+info)

Effect of age on urinary excretion of N-acetyl-beta-D-glucosaminidase. (7/982)

To examine the relationship between the concentrations of urinary NAG and age, we measured ratios of urinary N-acetyl-beta-D-glucosaminidase (NAG) to urinary creatinine (NAG index) in 137 healthy subjects, aged from 19 to 88 years. The study is also designed to evaluate the relationship between urinary NAG and blood pressure. The subjects were divided into 7 subgroups, according to their age (< 30, 30-39, 40-49, 50-59, 60-69, 70-79, 80 or more years). There was a positive correlation between NAG index and age (r = 0.36; P < 0.001). The regression equation relating NAG index (y) to age (x) was y = 0.065x + 0.97. The mean NAG indexes for the 7 subgroups divided by age were significantly different (P < 0.01). There was a positive correlation between NAG index and systolic blood pressure (r = 0.18; P < 0.05), but was not between diastolic blood pressure and NAG index. In multiple regression analysis, age and BUN significantly correlated with NAG index (r = 0.32; P < 0.01, r = 3.3; P = 0.07, respectively), although there was no correlation between systolic blood pressure and NAG index. This cross-sectional study showed a clear elevation in NAG index with age. The rate of elevation was 0.65 per decade. Urinary excretion of NAG may be unrelated to blood pressure.  (+info)

A quantitative study of pinocytosis and lysosome function in experimentally induced lysosomal storage. (8/982)

The highly pinocytic epithelial cells of the visceral yolk sac from 17.5-day rat conceptuses were used as a model in which to induce engorgement of the vacuolar system by direct accumulation of substances that are not hydrolysed by lysosomal enzymes. The ultra-structural appearances of these cells in pregnant animals that 24-48h before had received intraperitoneal injections of Triton WR-1339, polyvinylpyrrolidone, dextran or sucrose revealed gross abnormalities that were confined to the vacuolar system; in comparison with normal tissue the number, and in some cases the size, of vacuoles was increased, leading to close packing within the apical cytoplasm and distortion of the normal rounded shape. By culturing yolk sacs in vitro, rates of ingestion of 125I-labelled polyvinylpyrrolidone and of 125I-labelled bovine serum albumin were determined, together with the rate of digestion of the labelled protein. The rates of exocytosis of 125I-labelled polyvinylpyrrolidone and of lysosomal enzymes were also determined. No significant differences between normal and highly vacuolated tissues were found. Apparently marked vacuolation of these cells by these agents is without significant effect on pinocytosis, exocytosis or intralysosomal proteolysis.  (+info)

Here, C. glutamicum was shown to possess N-acetylglucosaminidase activity that is encoded by cg3158/nagA 2 . Structurally, the NagA2 protein belongs to the family 3 glycoside hydrolases, and among these, the N-acetyl-β-D-glucosaminidases show a selective specificity for GlcNAc as substrate [47] with only few exceptions [48]. N-acetyl-β-D-glucosaminidase activity was assayed with 4-nitrophenyl N,N-diacetyl-β-D-chitobioside as substrate, and about 0.27 mM supported half-maximal activity. In comparison, NagZ from E. coli had a higher Km on the same substrate (0.43 mM) [49], whereas NagZ of B. subtilis showed an about two fold lower Km of 0.11 ± 0.0 mM with 4-methylumbelliferyl-β-GlcNAc as substrate [40].. The role of the NagA2 activity in C. glutamicum is still unclear. Analysis of the C. glutamicum transcriptome revealed that the nagA2 gene is transcribed as leaderless transcript with a relatively low RNA abundance [41]. It is not known whether nagA2 expression is regulated in C. ...
Buy NAG elisa kit, Canine N-Acetyl-beta-D-Glucosaminidase ELISA Kit-AAS02154.1 (MBS005370) product datasheet at MyBioSource, ELISA Kits
Many bacteria have evolved ways to interact with glycosylation functions of the immune system of their hosts. Streptococcus pyogenes [GAS (group A Streptococcus)] secretes the enzyme EndoS that cleaves glycans on human IgG and impairs the effector functions of the antibody. The ndoS gene, encoding EndoS, has, until now, been thought to be conserved throughout the serotypes. However, in the present study, we identify EndoS2, an endoglycosidase in serotype M49 GAS strains. We characterized EndoS2 and the corresponding ndoS2 gene using sequencing, bioinformatics, phylogenetic analysis, recombinant expression and LC-MS analysis of glycosidic activity. This revealed that EndoS2 is present exclusively, and highly conserved, in serotype M49 of GAS and is only 37% identical with EndoS. EndoS2 showed endo-β-N-acetylglucosaminidase activity on all N-linked glycans of IgG and on biantennary and sialylated glycans of AGP (α1-acid glycoprotein). The enzyme was found to act only on native IgG and AGP and to be
The effect of short-term (mean 2.4 months), low-dose (5 mg/kg) cyclosporin A (CyA) on renal function and blood pressure was studied in eight patients with psoriasis. Studies were undertaken before, during and after treatment. Glomerular filtration rate (GFR) post-treatment was significantly higher than pretreatment or during treatment (pre, 119 +/- 7; during, 113 +/- 9; post, 133 +/- 11 ml/min per 1.73 m2; pre vs. during, NS; during vs. post, P , 0.01; pre vs. post, P , 0.05); effective renal plasma flow (ERPF) was unchanged (pre, 515 +/- 38; during, 485 +/- 49; post, 560 +/- 45 ml/min per 1.73 m2). There was no change in the urinary excretion of either albumin or the enzymes N-acetyl-glucosaminidase, lactate dehydrogenase, alanine aminopeptidase and alkaline phosphatase. There was a decrease in exchangeable sodium which persisted post-treatment (pre, 56 +/- 3; during, 49 +/- 3; post, 49 +/- 3 mmol/kg LBM; pre vs. during, P = 0.07; during vs. post, NS; pre vs. post, P = 0.06). Blood pressure ...
Endo-β-N-Acetylglucosaminidases (ENGases) are highly useful biocatalysts that can be used to synthetically access a wide variety of defined homogenous N-linked glycoconjugates in a convergent manner. The synthetic efficiency of a selection of family GH85 ENGases was investigated as the structure of
N-acetyl β-glucosaminidase (EC 3.2.1.52); hyaluronidase (EC 3.2.1.35); [protein]-3-O-(GlcNAc)-L-Ser/Thr β-N-acetylglucosaminidase (EC 3.2.1.169 ...
Werries, E., Wollek, E., Gottschalk, A. and Buddecke, E. (1969). „Separation of N-acetyl-α-glucosaminidase and N-acetyl-α-galactosaminidase from ox spleen. Cleavage of the O-glycosidic linkage between carbohydrate and polypeptide in ovine and bovine submaxillary glycoprotein by N-acetyl-α-galactosaminidase". Eur. J. Biochem. 10: 445-449. PMID 5348072 ...
β-galactosidase (EC 3.2.1.23); exo-β-glucosaminidase (EC 3.2.1.165); exo-β-1,4-galactanase (EC 3.2.1.-); β-1,3-galactosidase (EC 3.2.1.- ...
function nag_correg_robustm_corr_user_example indm = int64(1); x = [3.4, 6.9, 12.2; 6.4, 2.5, 15.1; 4.9, 5.5, 14.2; 7.3, 1.9, 18.2; 8.8, 3.6, 11.7; 8.4, 1.3, 17.9; 5.3, 3.1, 15; 2.7, 8.1, 7.7; 6.1, 3, 21.9; 5.3, 2.2, 13.9]; a = [1; 0; 1; 0; 0; 1]; theta = [0; 0; 0]; user = [4, 2]; [user, covar, aOut, wt, thetaOut, nit, ifail] = ... nag_correg_robustm_corr_user(@ucv, indm, x, a, theta, user, user) function [userp, u, w] = ucv(t, userp) cu = userp(1); u = 1.0; if (t ~= 0) t2 = t*t; if (t2 , cu) u = cu/t2; end end % w function and derivative cw = userp(2); if (t , cw) w = cw/t; else w = 1.0; end ...
Mucopolysaccharidosis type IIIB (MPS IIIB, Sanfilippo syndrome type B) is a lysosomal storage disorder caused by deficiency of the enzyme N-acetyl-alpha-D-glucosaminidase (NAGLU). Information on the natural course of MPS IIIB is scarce but much needed in view of emerging therapies. To improve knowledge on the natural course, data on all 52 MPS IIIB patients ever identified by enzymatic studies in the Netherlands were gathered. Clinical data on 44 patients could be retrieved. Only a small number (n = 9; 21%) presented with a classical MPS III phenotype; all other patients showed a much more attenuated course of the disease characterized by a significantly slower regression of intellectual and motor abilities. The majority of patients lived well into adulthood. First signs of the disease, usually mild developmental delay, were observed at a median age of 4 years. Subsequently, patients showed a slowing and eventually a stagnation of development. Patients with the attenuated phenotype had a stable ...
Learn more about Mucopolysaccharidosis Type Iiib from related diseases, pathways, genes and PTMs with the Novus Bioinformatics Tool.
Press Release issued Aug 16, 2016: The report provides comprehensive information on the Alpha-N-Acetylglucosaminidase (N-Acetyl-Alpha-Glucosaminidase or NAG or EC 3.2.1.50), targeted therapeutics, complete with analysis by indications, stage of development, mechanism of action (MoA), route of administration (RoA) and molecule type. The report also covers the descriptive pharmacological action of the therapeutics, its complete research and development history and latest news and press releases. Additionally, the report provides an overview of key players involved in Alpha-N-Acetylglucosaminidase (N-Acetyl-Alpha-Glucosaminidase or NAG or EC 3.2.1.50) targeted therapeutics development and features dormant and discontinued projects.
Sanfilippo syndrome, or Mucopolysaccharidosis (MPS) III, is a rare lysosomal storage disease (LSD) caused by loss in activity of 1 of 9 enzymes necessary for degradation of the glycosaminoglycan (GAG) heparan sulfate (HS) in lysosomes. MPS IIIA results from deficiency of the enzyme heparan N-sulfatase (sulfamidase). In the absence of this enzyme, intermediates of the HS degradation process accumulate in the lysosomes of neurons and glial cells, with lesser accumulation outside the brain. MPS IIIA symptoms arise on average at 7 months of age, with the average age of diagnosis at 4.5 years for the majority of patients. Patients present a wide spectrum and severity of clinical symptoms. The central nervous system (CNS) is the most severely affected organ system in patients with MPS IIIA, evidenced by deficits in language development, motor skills, and intellectual development. In addition, there are abnormal behaviors including but not limited to aggression and excess motor activity/hyperactivity ...
A new species, Porphyromonas gulae sp. nov., is proposed to include strains isolated from the gingival sulcus of various animal hosts which are distinct from related strains of Porphyromonas gingivalis of human origin. This bacterium exhibits the following characteristics: black-pigmented colonies; asaccharolytic, obligate anaerobic growth; and Gram-negative, non-motile and non-spore-forming, rod-shaped cells. Colonies do not fluoresce under UV light. Vitamin K1 and haemin are required for growth. Cells haemagglutinate sheep erythrocytes. Major fatty acid end products are butyric acid, isovaleric acid, succinic acid and phenylacetic acid. Strains are catalase-positive and indole is produced. Alkaline phosphatase, trypsin-like and N-acetyl-beta-glucosaminidase activities are strong. A beta-galactosidase and a glutamylglutamic acid arylamidase are also present. The G+C content of the chromosomal DNA is 51 mol%. DNA-DNA homology data and 16S rRNA gene sequence analysis provide strong evidence that strains
Regulatory News: Lysogene (Paris:LYS) (FR0013233475 - LYS), a leading, biopharmaceutical company pioneering gene therapy technologies to treat central nervous system diseases, today
Bei dieser Erkrankung haben betroffene Hunde Symptome wie unwillkürliches Muskelzittern und Gleichgewichtsstörungen, die sich immer weiter verschlimmern.
Evidence has been provided that the immunological mechanism is involved in the genesis or maintenance of hypertension. In the present study, we investigated the effects of interferon gamma, a potent immunomodulator derived from lymphocytes, on hypertension and organ damage in Dahl salt-sensitive rats and in spontaneously hypertensive rats. Subcutaneous injection of interferon gamma (5 x 10(4) units/kg body wt once a week for 10 weeks) reduced blood pressure in Dahl salt-sensitive rats fed a 4% high salt diet (174 versus 194 mm Hg, p less than 0.025). This blood pressure reduction was associated with an improvement of renal functions, an increase in glomerular filtration rate (690 versus 569 ml/day/100 g body wt, p less than 0.05), and decreases in urinary protein excretion (48 versus 78 mg/day/100 g body wt, p less than 0.025) and urinary N-acetyl-beta-D-glucosaminidase excretion (143 versus 183 milliunits/day/100 g body wt, p less than 0.05). Morphological investigation showed a marked ...
NAGLU Antibody (monoclonal) (M02), Mouse monoclonal antibody raised against a partial recombinant NAGLU. validated in WB (AT2970a), Abgent
We dont really play each other because hes younger than me. And he doesnt really play anymore. He just dribbles at home, Dylan said.. Garrett is slowly losing his motor skills, his speech, and his ability to understand. The 8-year-old has a rare genetic disorder.. Mucopolysaccharidoses, or MPSIII or Sanfilippo Syndrome Type IIIA, Scott said. Theres no cure, theres no treatment. And thats what he is dealing with.. His mother Kelli said, He fights the fight and smiles every day.. Garrett has a support network and a fan club at Alcoa Elementary School.. Theyre like the second parents. When we are not with him we trust them totally with Garrett and they love him, Scott said.. They love him and it shows. He is kind of the mayor of the school and beyond.. Everywhere we go and everyone we see most people around this area know who he is. We dont know them but they know him. So its remarkable, Kelli said.. We walk in the park and people come up and say hi Garrett and I dont even I ...
keratanase II: an endo-beta-N-acetylglucosaminidase; cleaves the beta(1-3)-glycosidic bond of a fucosylated 6-O-sulfated N-acetylglucosamine
Dr. Haiyan Fu. Dr. Fu has developed an efficient gene therapy procedure to treat MPSIIIB. We have made an AAV9 vector that has the ability to cross the blood-brain-barrier. This AAV9 vector carries the gene for NAGLU, the enzyme missing in MPSIIIB patients. By a singly intravenous injection of this AAV9-NAGLU vector, we were able to restore the NAGLU enzyme activity and correct the lysosomal storage pathology throughout the brain, spinal cord and multiple somatic tissues in adult MPSIIIB mice. Most importantly, the AAV9-vector-treated mice showed significant behavioral improvement and survived to a normal lifespan. In addition, this approach is minimally invasive and the IV injection itself has minimal risk to patients. With the generous support from the Sanfilippo families and friends through Bens Dream - The Sanfilippo Research Foundation, the experiments of this project are still ongoing.. We believe that we are in a very good position to move our AAV9-gene-therapy approach to clinical ...
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Chitosan-degrading fungal strain, Penicillium sp. IB-37-2A, produced mainly extracellular chitosanolytic enzymes under submerged agitating cultivation in p
Health, ...Investigators at Nationwide Childrens have received a grant from the ...Mucopolysaccharidosis (MPS) IIIB also known as Sanfilippo Syndrome B... To date the greatest challenge in developing therapies for MPS IIIB ...For more than a decade Dr. Fus team in the Center for Gene Therapy i...,NIH,grant,for,the,move,toward,clinical,trials,targeting,the,lysosomal,storage,disease,MPSIIIB,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
β-N-Acetylhexosaminidasef is a recombinant protein fusion of β-N-Acetyl-hexosaminidase and maltose binding protein. It has identical activity to β-N-Acetyl-hexosaminidase. β-N-Acetyl-hexosaminidasef catalyzes the hydrolysis of terminal β-D-N-acetyl-galactosamine and glucosamine residues from oligosaccharides. β-N-Acetyl-hexosaminidasef can be used to remove O-GlcNAc (N-Acetylglucosaminidase on serine/threonine).
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Takayuki Furuishi , Yukiko Kato , Toshiro Fukami , Toyofumi Suzuki , Tomohiro Endo , Hiromasa Nagase , Haruhisa Ueda , Kazuo Tomono ...
... - HIPOTERMIA TERAPEUTYCZNA PO NAGŁYM ZATRZYMANIU KRĄŻENIA. - ZALETY przeciwwskazania oraz korzyści ze stosowania hipotermii. W ramach Programu stosowane są
... - HIPOTERMIA TERAPEUTYCZNA PO NAGŁYM ZATRZYMANIU KRĄŻENIA. - ZALETY przeciwwskazania oraz korzyści ze stosowania hipotermii. W ramach Programu stosowane są
At least 118 mutations in the NAGLU gene have been found to cause mucopolysaccharidosis type IIIB (MPS IIIB). Most of these mutations change single DNA building blocks (nucleotides) in the gene. All of the mutations that cause MPS IIIB reduce or eliminate the function of alpha-N-acetylglucosaminidase.. The lack of alpha-N-acetylglucosaminidase activity disrupts the breakdown of a subset of GAGs called heparan sulfate. As a result, partially broken down heparan sulfate accumulates within lysosomes. Researchers believe that the accumulation of GAGs interferes with the functions of other proteins inside the lysosomes and disrupts the normal functions of cells. It is unknown why the buildup of heparan sulfate mostly affects the central nervous system in MPS IIIB. ...
Update from the laboratory of Dr. Elizabeth Neufield - November 2011. Our studies of the Sanfilippo syndrome (MPS III) continue along two lines - what is wrong in the brain and how can we treat it? We use the MPS IIIB mouse model for these studies. The primary defect in MPS IIIB is a mutation in the gene (Naglu) encoding ?-N-acetylglucosaminidase, one of the lysosomal enzymes needed for the breakdown of heparan sulfate. As a result, there is a deficiency of α-N-acetylglucosaminidase and heparan sulfate accumulates in lysosomes. But in addition, there is a small area of the brain (the medial entorhinal cortex or MEC) where a number of apparently unrelated substances accumulate; one of these also accumulates in another small area, the dentate gyrus. The medial entorhinal cortex and the dentate gyrus, which are connected to each other, are involved in learning and memory. While our primary study system is the mouse model of MPS IIIB, we have found similar pathology in the brain of the mouse model ...
Sanfilippo Syndrome (MPS III), an autosomal recessive hereditary disorder, is characterized by severe mental deterioration, mild physical defects and the excretion of heparan sulfate in the urine. There are four types of Sanfilippo Syndrome; types A and B are the most common forms.
In Sanfilippo syndrome, mucopolysaccharides are stored primar ily in the nerve system. There are four different enzyme deficiencies that cause Sanfilippo syndrome and hence the syndrome is classified as type A, B, C or D.. Type A is considered the most severe form, type B consists of both a milder and more severe form, and type C is considered to lie between types A and B in severity. Type D is very uncommon. The children are born healthy and develop normally up to between 2 and 6 years of age, after which developmental disorders, such as delayed speech and language development, and autistic traits appear.. Extreme hyperactivity is common. Thereafter, a progressive deter ioration occurs. Gradually mental retardation becomes apparent. In the next phase, balance worsens, as well as the ability to walk and mental capabilities. Epilepsy may occur. Skeletal deformities and stiffness of the joints may occur. Respiratory tract infections are common. Ear infections may cause hear ing impairments. ...
Drugs used in Alzheimers disease may result in a treatment for a rare genetic brain disease in babies called Sanfilippo syndrome. The new research suggests that new Alzheimers drugs may provide treatment for the currently untreatable metabolic disorder. Sanfilippo syndrome causes severe mental retardation and death
The MPS Family of Diseases. There is a continuous process in the body of replacing used materials and breaking them down for disposal. Children born with Mucopolysaccharide or MPS are unable to produce one of the enzymes essential for this process. Used materials cannot be broken down and remain stored in the cells of the body.. Babies born with one of the nine forms of MPS may show no sign of the disease, but as more and more cells become damaged by the storage of used material, symptoms begin to appear. Sadly the MPS family of disease is progressive which lead to an increase in symptoms and the problems they create as the years go by.. Sanfilippo Syndrome. Sanfilippo Syndrome is one of nine mucopolysaccharide diseases. Also known as MPS lll, it takes its name from Dr. Sanfilippo who was one of the doctors who first described the condition in 1963. MPS III is subdivided into 4 similar subtypes. Sophie suffers from the particular subtype MPS III B.. How does the disease progress?. Children begin ...
Sanfilippo Syndrome is within a set of neurodegenerative diseases called tauopathies (the most common of which is Alzheimers Disease). Although there are 4 subtypes of Sanfilippo Syndrome, they are all characterized by reduced degradation of heparan sulfate (see Hurler Syndrome section) due to reduced levels of a lysosomal enzyme. It was found in a mouse model (MPS IIIB) that there were significantly increased levels of the protein lysozyme; increased levels of this disease were found to cause the creation of hyperphosphorylated tau which is found in the brains of Alzheimers patients and patients with other tauopathies. Significant research is being done in Alzheimers disease which may carry over to Sanfilippo Syndrome as well due to their similarities. 1. 4 different subtypes: Each Sanfilippo subtype is caused by the deficiency of each specific enzyme: heparin N-sulfatase for MPS-III A, N-acetyl-alpha-D-glucosaminidase for MPS-III B, acetyl-CoA: alpha-glucosaminide acetyltransferase for ...
Systemic Biopotency demonstrated time- and dose-dependent reductions of disease causing Heparan Sulfate in the Cerebrospinal fluid (CSF) and liver volumes. --Preservation of deep brain architecture observed after intravenous administration --Stabilization of neurocognitive assessment scores at one year post-injection. NEW YORK and CLEVELAND, Oct. 06, 2017 (GLOBE NEWSWIRE) -- Abeona Therapeutics Inc. (NASDAQ:ABEO), a leading clinical-stage biopharmaceutical company focused on developing novel gene and cell therapies for life-threatening rare diseases, announced one year data from Cohort 1 of the ongoing ABO-102 Phase 1/2 trial for Sanfilippo syndrome Type A (MPS IIIA). The results demonstrated robust and durable clinical effects achieved one year post-administration, with significant reductions in biopotency and biophysical measures, preservation of deep brain architecture, and stabilization across multiple neurocognitive assessments reported in comparison to untreated control subjects. The ...
1. Slámová K, Bojarová P, Petrásková L. et al. β-N-Acetylhexosaminidase: Whats in a name…?. Biotechnol Adv. 2010;28:682-93 2. Merzendorfer H, Zimoch L. Chitin metabolism in insects: structure, function and regulation of chitin synthases and chitinases. J Exp Biol. 2003;206:4393-412 3. Nagamatsu Y, Yanagisawa I, Kimoto M. et al. Purification of a chitooligosaccharidolytic β-N-acetylglucosaminidase from Bombyx mori larvae during metamorphosis and the nucleotide sequence of its cDNA. Biosci Biotechnol Biochem. 1995;59:219-25 4. Yang Q, Liu T, Liu F. et al. A novel β-N-acetyl-D-hexosaminidase from the insect Ostrinia furnacalis (Guenée). FEBS J. 2008;275:5690-702 5. Liu T, Zhang H, Liu F. et al. Structural determinants of an insect β-N-acetyl-D-hexosaminidase specialized as a chitinolytic enzyme. J Biol Chem. 2011;286:4049-58 6. Zheng YP, Krell PJ, Doucet D. et al. Cloning, expression, and localization of a molt-related β-N-acetylglucosaminidase in the Spruce budworm, Choristoneura ...
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Staphylococcus aureus; pan ID: SAUPAN004467000; products: autolysin, N-acetylmuramoyl-L-alanine amidase, mannosyl-glycoprotein endo-beta-N-acetylglucosamidase, beta-N-acetylglucosaminidase, putative, exported protein, family 4 N-acetylmuramoyl-L-alanine amidase, mannosyl-glycoendo-beta-N-acetylglucosaminidase family protein, mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase, mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase family protein, mannosyl-glycoprotein endo-beta-N-glucosaminidase, peptidoglycan endo-beta-N-acetylglucosaminidase
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
Gene therapy shows potential to effectively target the underlying cause of Sanfilippo syndrome and delay or prevent neurodegeneration, a review study finds.
Building Biotech is a semi-formal event hosted at the beautiful Hyatt Regency Vancouver. Students will have the opportunity to hear about exciting developments in biotechnology while networking with industry leaders in an intimate dinner setting. Join other students and industry professionals to find the tools and resources essential to starting your career, information about emerging trends in bioenergy, and the personal paths to success taken by two pairs of local entrepreneurs.. To kick-off our new season, the SBN is excited to present Building Biotech: Inspiring Innovators and Building the Bioeconomy, with keynote speaker Dr. Ernie McEachern, the CEO and co-founder of Alectos Therapeutics Inc. Alectos Therapeutics Inc. has recently announced a research collaboration with Merck to identify and develop compounds that modulate O-linked N-acetylglucosaminidase (O-GlcNAcase), an enzyme that is believed to be involved in the development of Alzheimers disease. Other speakers include Drs. Euan ...
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An alternative microRNA-mediated post-transcriptional regulation of GADD45A by p53 in human non-small-cell lung cancer cells Sci Rep 2017 [AGO2 ...
Repurposing of Proton Pump Inhibitors as first identified small molecule inhibitors of endo-β-N-acetylglucosaminidase (ENGase) for the treatment of NGLY1 deficiency, a rare genetic disease Bioorg. Med. Chem. Lett. 2017 [ENGASE ...
2016) Neurological Correction of Mucopolysaccharidosis IIIB Mice by Haematopoietic Stem Cell Gene Therapy. In: 19th Annual Meeting of the American-Society-of-Gene-and-Cell-Therapy (ASGCT), 04 May 2016 - 07 May 2016, Washington, DC. ...
Our children are born with a genetic defect passed on from each parent that results in their body being unable to break down and recycle natural cellular waste due to a missing enzyme. Because the waste isnt broken down, it builds up in their brains, taking away all their skills and knowledge until they pass away in their early teens.
Buy anti-NAGase antibody, Rabbit anti-Rat N-Acetyl Beta-D-Glucosaminidase (NAGase) Polyclonal Antibody-NP_571979.1 (MBS2001439) product datasheet at MyBioSource, Primary Antibodies. Application: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
... DAVIS, Calif., July 14, 2003 - SoftIntegration, Inc. and the Numerical Algorithms Group (NAG) announce the release of Ch NAG Statistics Package version 1.0. Designed to meet the needs of a wide range of researchers seeking rapid statistical application development, the Ch NAG Statistics Package makes a set of the robust NAG statistical routines available to users of Ch, an embeddable C/C++ interpreter for cross-platform scripting, shell programming, 2D/3D plotting, numerical computing, and embedded scripting. Ch NAG Statistics Package makes the rigorously tested statistical routines in the NAG C library available to Ch users without traditional coding and linking. This comprehensive selection of statistical routines can readily use the 2D/3D graphical plotting capabilities of Ch. In addition, with SoftIntegrations free Ch ODBC and Ch CGI toolkit, it is easy to integrate with databases and create web-based applications for statistical ...
Further evidence of a hyperactive ?-N-acetylglucosaminidase-producing allele". Clinical Genetics. 41 (5): 243-247. doi:10.1111/ ...
N-acetylglucosaminidase, alpha is a protein that in humans is encoded by the NAGLU gene. This gene encodes an enzyme that ... N-acetylglucosaminidase, alpha". Weber B, Blanch L, Clements PR, Scott HS, Hopwood JJ (June 1996). "Cloning and expression of ... "Purification and partial characterization of alpha-N-acetylglucosaminidase from human liver". Journal of Biochemistry. 110 (5 ...
... alpha-N-acetylglucosaminidase (type B; MIM 252920); acetyl CoA:alpha-glucosaminide acetyltransferase (type C; MIM 252930); and ...
N-Acetylglucosaminidase: Experimentally found within mouse cortical granules, N-Acetylglucosaminidase is a glycosidase that ... Therefore, N-Acetylglucosaminidase contributes to polyspermy prevention. p32: The name, p32, refers to the protein's molecular ...
NAGLU: N-acetyl glucosaminidase, Sanfilippo B syndrome (17q21.2). *SLC4A1: Band 3 anion transporter protein. Solute carrier ...
N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase is an enzyme that in humans is encoded by the NAGPA gene. ... "Entrez Gene: NAGPA N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase". Lee JK, Pierce M (1995). "Purification ... "Purification and characterization of human lymphoblast N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase". ... and functional expression of two splice forms of human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase". J ...
... produces endo-β-N-acetylglucosaminidase mithramycin, amicetin, bamicetin and plicacetin. ., Aghighi S ... "High-level expression of endo-β-N-acetylglucosaminidase H from Streptomyces plicatus in Pichia pastoris and its application for ...
Sanfilippo B is caused by the missing or deficient enzyme alpha-N-acetylglucosaminidase. Sanfilippo C results from the missing ...
von Figura, K. (1977). „Human α-N-acetylglucosaminidase. 1. Purification and properties". Eur. J. Biochem. 80: 523-533. PMID ... von Figura, K. (1977). „Human α-N-acetylglucosaminidase. 2. Activity towards natural substrates and multiple recognition forms ... Weissmann, B., Rowen, G., Marshall, J. and Friederici, D. (1967). „Mammalian α-acetylglucosaminidase. Enzymic properties, ...
... β-N-acetylglucosaminidase, and β-N-acetylgalactosaminidase from calf brain". Biochemistry. 6: 2775-2782. doi:10.1021/ ...
... cytosolic beta-N-acetylglucosaminidase from human brain". J. Biol. Chem. 276 (13): 9838-45. doi:10.1074/jbc.M010420200. PMID ... "Analysis of MGEA5 on 10q24.1-q24.3 encoding the beta-O-linked N-acetylglucosaminidase as a candidate gene for type 2 diabetes ... a cytoplasmic hyaluronidase and a beta-N-acetylglucosaminidase". Biochem. Biophys. Res. Commun. 283 (3): 634-40. doi:10.1006/ ... further characterization of the nucleocytoplasmic beta-N-acetylglucosaminidase, O-GlcNAcase". J. Biol. Chem. 277 (3): 1755-61. ...
Tachibana Y, Yamashita K, Kobata A (1982). "Substrate specificity of mammalian endo-beta-N-acetylglucosaminidase: study with ...
... endo-beta-N-acetylglucosaminidase D, endo-beta-N-acetylglucosaminidase F, endo-beta-N-acetylglucosaminidase H, endo-beta-N- ... di-N-acetylchitobiosyl beta-N-acetylglucosaminidase, endo-beta-acetylglucosaminidase, endo-beta-(1->4)-N-acetylglucosaminidase ... Chien, S.; Weinburg, R.; Li, S.; Li, Y. (1977). "Endo-β-N-acetylglucosaminidase from fig latex". Biochem. Biophys. Res. Commun ... The enzyme Endoglycosidase H (EC 3.2.1.96, Endo-β-N-acetylglucosaminidase H, N,N'-diacetylchitobiosyl beta-N- ...
Alpha-N-acetylglucosaminidase is a lysosomal enzyme required for the stepwise degradation of heparan sulphate. Mutations on the ... Glycoside hydrolase family 89 CAZY GH_89 includes enzymes with α-N-acetylglucosaminidase EC 3.2.1.50 activity. The enzyme ... "Mouse model of Sanfilippo syndrome type B produced by targeted disruption of the gene encoding alpha-N-acetylglucosaminidase". ... alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B ...
Frohwein YZ, Gatt S (September 1967). "Isolation of β-N-acetylhexosaminidase, β-N-acetylglucosaminidase, and β-N- ... cytosolic beta-N-acetylglucosaminidase from human brain". J. Biol. Chem. 276 (13): 9838-45. doi:10.1074/jbc.M010420200. PMID ... beta-N-acetylglucosaminidase, hexosaminidase A, N-acetylhexosaminidase, beta-D-hexosaminidase) is an enzyme involved in the ... for β-N-acetylglucosaminidase, β-N-acetylhexosaminidase and β-N-acetylgalactosaminidase". Biochem. J. 261 (3): 1059-60. PMC ...
Functionally, five types of lysin catalytic domain can be distinguished: Endo-β-N-acetylglucosaminidase N-acetylmuramidase ( ... Endo-β-N-acetylglucosaminidase lysins cleave NAGs while N-acetylmuramidase lysins (lysozyme-like lysins) cleave NAMs. ...
... CAZY GH_73 includes peptidoglycan hydrolases with endo-β-N-acetylglucosaminidase specificity. ...
... enzymes have endo-beta-N-acetylglucosaminidase activity EC 3.2.1.96 (CAZY GH_85). These enzymes ...
... cytosolic β-N-acetylglucosaminidase from human brain". J. Biol. Chem. 276: 9838-9845. doi:10.1074/jbc.M010420200. PMID 11148210 ... further characterization of the nucleocytoplasmic β-N-acetylglucosaminidase, O-GlcNAcase". J. Biol. Chem. 277 (3): 1755-1761. ...
The NAGLU gene, located on chromosome 17q21.2, encodes alpha-N-acetylglucosaminidase, deficiency of which results in MPS IIIB. ...
... s (chitodextrinase, 1,4-beta-poly-N-acetylglucosaminidase, poly-beta-glucosaminidase, beta-1,4-poly-N-acetyl ... The regulation of an endochitinase in Trichoderma atroviride is dependent on a N-acetylglucosaminidase, and the data indicates ... β-1,4- N-acetylglucosaminidases (EC 3.2.1.30) split the multimer products, such as di-acetylchitobiose, chitotriose, and ... Family 20 includes N-acetylglucosaminidase and a similar enzyme, N-acetylhexosaminidase.[9] ...
... produces secalonic acid D, chitinase, oxalic acid, oxaline and β-N-acetylglucosaminidase and occurs ...
CBM32 modules are associated with catalytic modules such as sialidases, B-N-acetylglucosaminidases, α-N-acetylglucosaminidases ...
... of a cosmid from a gene-rich region in 17q21 and characterization of a candidate gene for alpha-N-acetylglucosaminidase with ...
... s (EC 3.2.1.14, chitodextrinase, 1,4-beta-poly-N-acetylglucosaminidase, poly-beta-glucosaminidase, beta-1,4-poly-N- ...
Alpha-N-acetylglucosaminidase (EC 3.2.1.50, alpha-acetylglucosaminidase, N-acetyl-alpha-D-glucosaminidase, N-acetyl-alpha- ... von Figura, K. (1977). "Human α-N-acetylglucosaminidase. 1. Purification and properties". Eur. J. Biochem. 80: 523-533. doi: ... von Figura, K. (1977). "Human α-N-acetylglucosaminidase. 2. Activity towards natural substrates and multiple recognition forms ... Weissmann, B.; Rowen, G.; Marshall, J.; Friederici, D. (1967). "Mammalian α-acetylglucosaminidase. Enzymic properties, tissue ...
In enzymology, a beta-aspartyl-N-acetylglucosaminidase (EC 3.2.2.11) is an enzyme that catalyzes the chemical reaction 1-beta- ... Eylar EH; Murakami M (1966). "beta-Aspartyl-N-acetylglucosaminidase from epididymis". Methods Enzymol. Methods in Enzymology. 8 ...
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase/chemistry*. *Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase/ ... Endo-beta-N-acetylglucosaminidase from Mucor hiemalis (Endo-M), a family 85 glycoside hydrolase, acts on the beta1,4 linkage of ... This is the first glycosynthase derived from endo-beta-N-acetylglucosaminidases that proceed via a substrate-assisted mechanism ... Mutants of Mucor hiemalis endo-beta-N-acetylglucosaminidase show enhanced transglycosylation and glycosynthase-like activities. ...
Characterization of AcmB, an N-acetylglucosaminidase autolysin from Lactococcus lactis.. Huard C1, Miranda G, Wessner F, ... Though showing sequence similarity with enterococcal muramidase, AcmB has N-acetylglucosaminidase specificity. The acmB gene ...
N-Acetylglucosaminidase activity, a functional trait of chitin degradation, is regulated differentially within two orders of ... Because extracellular N-acetylglucosaminidase activity has been proposed as functional trait of chitin degradation, we screened ... Chitin degradation Chitinase N-Acetylglucosaminidase Functional trait Fungal phylogeny Electronic supplementary material. The ... Table S.2 Extracellular N-acetylglucosaminidase activity (pmol.ml-1.min-1) of ectomycorrhizal fungi with and without chitin in ...
Compare and order N-Acetylglucosaminidase, alpha ELISA Kits. View citations, images, detection ranges, sensitivity, prices and ... alpha-N-acetylglucosaminidase , N-acetylglucosaminidase, alpha- , alpha-N-acetylglucosaminidase-like , N-acetylglucosaminidase ... More product categories related to N-Acetylglucosaminidase, alpha ELISA Kit * 14 N-Acetylglucosaminidase, alpha ELISA & Assay ... alpha- (Sanfilippo disease IIIB) , N-acetyl-glucosaminidase , alpha-N-acetylglucosaminidase, lysosomal , N-acetyl-alpha- ...
Antibodies for proteins involved in N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase activity pathways, ... Antibodies for proteins involved in N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase activity pathways; ...
β-N-Acetylglucosaminidase from Streptococcus pneumoniae, recombinant, expressed in E. coli, buffered aqueous solution pricing ... β-N-Acetylglucosaminidase from Canavalia ensiformis (Jack bean), ammonium sulfate suspension, ≥15 units/mg protein pricing ... β-N-Acetylglucosaminidase from bovine kidney, ammonium sulfate suspension, 10-50 units/mg protein pricing ... Insulin-like growth factor II peptide fusion enables uptake and lysosomal delivery of α-N-acetylglucosaminidase to ...
Endo-β-N-Acetylglucosaminidases (ENGases) are highly useful biocatalysts that can be used to synthetically access a wide ... Endo-β-N-Acetylglucosaminidases (ENGases) are highly useful biocatalysts that can be used to synthetically access a wide ... Endo-β-N-Acetylglucosaminidase catalysed glycosylation: tolerance of enzymes to structural variation of the glycosyl amino acid ... Endo-β-N-Acetylglucosaminidase catalysed glycosylation: tolerance of enzymes to structural variation of the glycosyl amino acid ...
β-N-Acetylglucosaminidase from Streptococcus pneumoniae from AG Scientific, the leading supplier of biochemicals. ... β-N-Acetylglucosaminidase (NAG) is a lysosomal enzyme that is expressed in various tissues, including kidney, liver and lungs. ...
Additionally, the report provides an overview of key players involved in Alpha-N-Acetylglucosaminidase (N-Acetyl-Alpha- ... The report provides comprehensive information on the Alpha-N-Acetylglucosaminidase (N-Acetyl-Alpha-Glucosaminidase or NAG or EC ... Alpha-N-Acetylglucosaminidase (N-Acetyl-Alpha-Glucosaminidase or NAG or EC 3.2.1.50) - Pipeline Review, H1 2016. The Report ... The report reviews latest news and deals related to Alpha-N-Acetylglucosaminidase (N-Acetyl-Alpha-Glucosaminidase or NAG or EC ...
Distribution of Endo-N-acetylglucosaminidase Gene Sequences in Bifidobacteria. Based on known endo-N-acetylglucosaminidase ... Endo-β-N-acetylglucosaminidases were identified in certain isolates of Bifidobacterium longum subsp. longum, B. longum subsp. ... 2001) Molecular cloning and expression of endo-β-N-acetylglucosaminidase D, which acts on the core structure of complex type ... 2000) Production of an endo-β-N-acetylglucosaminidase activity mediates growth of Enterococcus faecalis on a high-mannose-type ...
The N-acetylglucosaminidase NagZ of Pseudomonas aeruginosa catalyzes the first cytoplasmic step in recycling of muropeptides, ... The N-acetylglucosaminidase NagZ of Pseudomonas aeruginosa catalyzes the first cytoplasmic step in recycling of muropeptides, ... Catalytic Cycle of the N-Acetylglucosaminidase NagZ from Pseudomonas aeruginosa. 01-08-2017 ...
... of peptidoglycan hydrolysis products obtained using the purified protein to show that AtlA is an N-acetylglucosaminidase. To ... Functional analysis of AtlA, the major N-acetylglucosaminidase of Enterococcus faecalis. Catherine Eckert 1, 2 Maxime Lecerf 1 ... Functional analysis of AtlA, the major N-acetylglucosaminidase of Enterococcus faecalis.. Journal of Bacteriology, American ... of peptidoglycan hydrolysis products obtained using the purified protein to show that AtlA is an N-acetylglucosaminidase. To ...
Effects of pentoxifylline administration on urinary N-acetyl-- glucosaminidase excretion in type 2 diabetic patients: A short- ...
2018-2025 Alpha N-Acetylglucosaminidase Report on Global and United States Market, Status and Forecast, by Players, Types and ... Global Alpha N-Acetylglucosaminidase Sales Market Report 2018. United States Alpha N-Acetylglucosaminidase Market Report 2018. ... 10.4 Alpha N-Acetylglucosaminidase Forecast by Type. 10.4.1 Global Alpha N-Acetylglucosaminidase Sales (K Pcs) and Revenue ( ... 2.1 Alpha N-Acetylglucosaminidase Product Overview. 2.2 Alpha N-Acetylglucosaminidase Market Segment by Type. 2.2.1 Lesinidase ...
Galactosyltransferase-dependent sialylation of complex and endo-N-acetylglucosaminidase H-treated core N-glycans in vitro. ... Incorporation of sialic acid into endo-beta-N-acetylglucosaminidase H-treated oligomannose glycoproteins may be useful for in ... Incorporation of sialic acid into endo-beta-N-acetylglucosaminidase H-treated oligomannose glycoproteins may be useful for in ... Galactosyltransferase-dependent sialylation of complex and endo-N-acetylglucosaminidase H-treated core N-glycans in vitro. FEBS ...
Novel Cancer Vaccination System Based on Human Endo-Β-N-Acetyl Glucosaminidase Gene Delivery. Satoshi Watanabe, Seiki Haraguchi ... Treatment of a cell with endo-?-N-acetylglucosaminidase (ENGase), an enzyme that cleaves the amide bond between the proximal ...
Carbohydrates - β-N-Acetyl-Glucosaminidase. Megazymes chromogenic substrates provide the most convenient methods for the assay ...
Beta-aspartyl-N-acetylglucosaminidase - In enzymology, a beta aspartyl N acetylglucosaminidase (EC number,3.2.2.11) is an ... Alpha-N-acetylglucosaminidase - N acetylglucosaminidase, alpha (Sanfilippo disease IIIB) Identifiers Symbol NAGLU Entrez 4669 ... α-N-acetylglucosaminidase - α N ac·e·tyl·glu·co·sa·min·i·dase (as″ə təl ) (as″ə tēl″gloo kōs″ə minґĭ dās) [EC 3.2.1.50] a ... β-D-acetylglucosaminidase - β D ac·e·tyl·glu·co·sa·min·i·dase (asґə təl ) (asґə tēl″gloo kōs″ə minґĭ dās) a lysosomal ...
"β-acetylglucosaminidase 18A, is an enzyme from Bacteroides thetaiotaomicron that participates in the endohydrolysis of the ... β-Acetylglucosaminidase 18A from Bacteroides thetaiotaomicron, Recombinant β-Acetylglucosaminidase 18A from Bacteroides ... β-acetylglucosaminidase 18A, is an enzyme from Bacteroides thetaiotaomicron that participates in the endohydrolysis of the ...
N-acetylglucosaminidase References. Clarke, V. A., N. Platt and T.D. Betters. Cloning and expression of the beta-N- ... N-acetylglucosaminidase cleaves all non-reducing terminal β-linked N-acetylglucosamine residues from complex carbohydrates and ... N-acetylglucosaminidase cleaves all non-reducing terminal β-linked N-acetylglucosamine residues from complex carbohydrates and ... Purity Each lot of N-acetylglucosaminidase is tested for contaminating protease as follows; 10 μg of denatured BSA is incubated ...
Compartment GO Terms for N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase. Bounding membrane of organelle ... HCA RNA Cell Line for N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase. ...
Endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae (Endo-A) had high levels of transglycosylation activity. The ... Endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae (Endo-A) had high levels of transglycosylation activity. The ... Endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae (Endo-A) had high levels of transglycosylation activity. The ... Endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae (Endo-A) had high levels of transglycosylation activity. The ...
β-acetylglucosaminidase 73A from Clostridium perfringens, Recombinant. β-acetylglucosaminidase 73A from Clostridium perfringens ...
  • N-acetylglucosaminidase (ENGase), an enzyme that cleaves the amide bond between the proximal GlcNAc residues at the side chain of an asparagine residue on N-glycans, also causes exposure of GlcNAc residues on the cell surface. (longdom.org)
  • N-acetylglucosaminidase cleaves all non-reducing terminal β-linked N-acetylglucosamine residues from complex carbohydrates and glycoproteins. (qa-bio.com)
  • The first gene was shown to encode a cell wall-associated β-N-acetylglucosaminidase (NagZ, formerly YbbD) that cleaves the terminal nonreducing N-acetylglucosamine of muropeptides and also accepts chromogenic or fluorogenic β-N-acetylglucosaminides. (uni-konstanz.de)
  • The N-acetylglucosaminidase NagZ of Pseudomonas aeruginosa catalyzes the first cytoplasmic step in recycling of muropeptides, cell-wall-derived natural products. (sebbm.es)
  • NagZ de Pseudomonas aeruginosa cataliza el primer paso del reciclaje de los muropéptidos (fragmentos naturales de la pared bacteriana) en el citoplasma y regula la expresión de la -lactamasa, la enzima clave en la resistencia a antibióticos -lactámicos. (sebbm.es)
  • El trabajo proporciona una imagen de todos los pasos del ciclo catalítico de NagZ en este importante patógeno humano. (sebbm.es)
  • Endo-beta-N-acetylglucosaminidase from Mucor hiemalis (Endo-M), a family 85 glycoside hydrolase, acts on the beta1,4 linkage of N,N'-diacetylchitobiose moiety in the N-linked glycans of glycoproteins and catalyzes not only the hydrolysis reaction but also the transglycosylation reaction that transfers the releasing sugar chain to an acceptor other than water to form a new glycosidic linkage. (nih.gov)
  • We have used tandem mass spectrometry analysis of peptidoglycan hydrolysis products obtained using the purified protein to show that AtlA is an N-acetylglucosaminidase. (inserm.fr)
  • By means of transglycosylation, (Man) 6 GlcNAc was transferred en bloc to the partially deglycosylated ovalbumin glycopeptide (EEKYN(GlcNAc) LTSVL) concomitant with the hydrolysis of (Man) 6 (GlcNAc) 2 Asn. (elsevier.com)
  • Endo-β-N-acetylglucosaminidase (ENGase) catalyzes hydrolysis of N-linked oligosaccharides. (elsevier.com)
  • The 'profile' of the epidermal glycosidases is significantly different from that reported for whole skin, the activities of beta-galactosidase and beta-acetylglucosaminidase being very high and those of the remaining enzymes relatively low in epidermis. (nih.gov)
  • Through studies on a representative member, the Nag3 beta-N-acetylglucosaminidase from Cellulomonas fimi, we now show that these enzymes act preferentially as glycoside phosphorylases. (ubc.ca)
  • O -GlcNAcylation is orchestrated by two opposing enzymes, O -GlcNAc transferase and OGA ( O -GlcNAcase or β-N-acetylglucosaminidase), which recognize their target proteins via as yet unidentified mechanisms. (biochemj.org)
  • Functional analysis of AtlA, the major N-acetylglucosaminidase of Enterococcus faecalis. (inserm.fr)
  • In enzymology, a beta-aspartyl-N-acetylglucosaminidase (EC 3.2.2.11) is an enzyme that catalyzes the chemical reaction 1-beta-aspartyl-N-acetyl-D-glucosaminylamine + H2O ⇌ {\displaystyle \rightleftharpoons } L-asparagine + N-acetyl-D-glucosamine Thus, the two substrates of this enzyme are 1-beta-aspartyl-N-acetyl-D-glucosaminylamine and H2O, whereas its two products are L-asparagine and N-acetyl-D-glucosamine. (wikipedia.org)
  • Colloidal chitin did not support growth of a strain secreting exochitinase ChiB and β-N-acetylglucosaminidase NagA2. (biomedcentral.com)
  • The Report Alpha-N-Acetylglucosaminidase (N-Acetyl-Alpha-Glucosaminidase or NAG or EC 3.2.1.50) - Pipeline Review, H1 2016 provides information on pricing, market analysis, shares, forecast, and company profiles for key industry participants. (sbwire.com)
  • Albany, NY -- ( SBWIRE ) -- 08/16/2016 -- Global Markets Directs, Alpha-N-Acetylglucosaminidase (N-Acetyl-Alpha-Glucosaminidase or NAG or EC 3.2.1.50) - Pipeline Review, H1 2016, provides in depth analysis on Alpha-N-Acetylglucosaminidase (N-Acetyl-Alpha-Glucosaminidase or NAG or EC 3.2.1.50) targeted pipeline therapeutics. (sbwire.com)
  • The report provides comprehensive information on the Alpha-N-Acetylglucosaminidase (N-Acetyl-Alpha-Glucosaminidase or NAG or EC 3.2.1.50), targeted therapeutics, complete with analysis by indications, stage of development, mechanism of action (MoA), route of administration (RoA) and molecule type. (sbwire.com)
  • Additionally, the report provides an overview of key players involved in Alpha-N-Acetylglucosaminidase (N-Acetyl-Alpha-Glucosaminidase or NAG or EC 3.2.1.50) targeted therapeutics development and features dormant and discontinued projects. (sbwire.com)
  • this report analyzes the top players in global and United States market, and splits the Alpha N-Acetylglucosaminidase market by product type and application/end industries. (rnrmarketresearch.com)
  • The global Alpha N-Acetylglucosaminidase market is valued at XX million USD in 2017 and is expected to reach XX million USD by the end of 2025, growing at a CAGR of XX% between 2017 and 2025. (rnrmarketresearch.com)
  • Any changes from United States might affect the development trend of Alpha N-Acetylglucosaminidase. (rnrmarketresearch.com)
  • Purified beta-N-acetylglucosaminide beta(1-4)galactosyltransferase and partially purified beta-galactoside alpha(2-6)-sialyltransferase were used to elongate and terminate glycan chains of agalacto-ovalbumin and endo-beta-N-acetylglucosaminidase H-treated yeast invertase in vitro. (uzh.ch)
  • Patients with Mucolipidosis II/III (I-cell disease) may demonstrate elevations of alpha-N-acetylglucosaminidase in addition to abnormalities of other hydrolases. (testcatalog.org)
  • Alpha-N-acetylglucosaminidase is involved in the step-wise breakdown of large molecules called glycosaminoglycans (GAGs). (medlineplus.gov)
  • Alpha-N-acetylglucosaminidase removes a sugar called N-acetylglucosamine when it is at the end of the GAG chain. (medlineplus.gov)
  • People with this type are missing or do not produce enough alpha- N -acetylglucosaminidase. (medlineplus.gov)
  • O -GlcNAc 1 is a dynamically regulated post-translational modification (PTM), in which a β- N -acetylglucosamine moiety is attached to hydroxyl side chains of serine or threonine residues of proteins by O -GlcNAc-transferase ( 1 ) and removed by β- N -acetylglucosaminidase ( O -GlcNAcase) ( 2 ). (mcponline.org)
  • Both the OGT ( O -GlcNAc transferase) and its antagonistic OGA ( O -GlcNAcase or β-N-acetylglucosaminidase) are encoded by single genes in metazoa [ 11 , 12 ]. (biochemj.org)
  • These profiles of extracellular N-acetylglucosaminidase stimulation/repression might be conserved at a high phylogenetic level in the Basidiomycota phylum, as illustrated by the opposite patterns of regulation between Boletales and Agaricales. (springer.com)
  • Furthermore, our results revealed two regulation patterns of extracellular N-acetylglucosaminidase activities. (springer.com)
  • Mutants of Mucor hiemalis endo-beta-N-acetylglucosaminidase show enhanced transglycosylation and glycosynthase-like activities. (nih.gov)
  • The synthesis of an analogue of 6-epi-valienamine bearing an acetamido group and its characterisation as an inhibitor of beta-N-acetylglucosaminidases are described. (edu.au)
  • CAZy glycoside hydrolase family GH3 consists primarily of stereochemistry-retaining beta-glucosidases but also contains a subfamily of beta-N-acetylglucosaminidases. (ubc.ca)
  • Los resultados publicados en Blood, proporcionan una visión de los mecanismos moleculares de la linfomagénesis asociada a la pérdida del gen CREBBP y demuestran la diferencia existente entre las mutaciones de CREBBP que se producen en el linfoma folicular en comparación con aquellas que aparecen en el linfoma difuso de células B grandes. (sebbm.es)