Acetylgalactosamine: The N-acetyl derivative of galactosamine.Asialoglycoprotein Receptor: A C-type lectin that is a cell surface receptor for ASIALOGLYCOPROTEINS. It is found primarily in the LIVER where it mediates the endocytosis of serum glycoproteins.Asialoglycoproteins: Endogenous glycoproteins from which SIALIC ACID has been removed by the action of sialidases. They bind tightly to the ASIALOGLYCOPROTEIN RECEPTOR which is located on hepatocyte plasma membranes. After internalization by adsorptive ENDOCYTOSIS they are delivered to LYSOSOMES for degradation. Therefore receptor-mediated clearance of asialoglycoproteins is an important aspect of the turnover of plasma glycoproteins. They are elevated in serum of patients with HEPATIC CIRRHOSIS or HEPATITIS.Molecular Mimicry: The structure of one molecule that imitates or simulates the structure of a different molecule.Mannose-Binding Lectin: A specific mannose-binding member of the collectin family of lectins. It binds to carbohydrate groups on invading pathogens and plays a key role in the MANNOSE-BINDING LECTIN COMPLEMENT PATHWAY.Pregnancy-Associated Plasma Protein-A: A product of the PLACENTA, and DECIDUA, secreted into the maternal circulation during PREGNANCY. It has been identified as an IGF binding protein (IGFBP)-4 protease that proteolyzes IGFBP-4 and thus increases IGF bioavailability. It is found also in human FIBROBLASTS, ovarian FOLLICULAR FLUID, and GRANULOSA CELLS. The enzyme is a heterotetramer of about 500-kDa.OrosomucoidSulfotransferases: Enzymes which transfer sulfate groups to various acceptor molecules. They are involved in posttranslational sulfation of proteins and sulfate conjugation of exogenous chemicals and bile acids. EC 2.8.2.Arylsulfotransferase: A sulfotransferase that catalyzes the sulfation of a phenol in the presence of 3'-phosphoadenylylsulfate as sulfate donor to yield an aryl sulfate and adenosine 3',5'-bisphosphate. A number of aromatic compounds can act as acceptors; however, organic hydroxylamines are not substrates. Sulfate conjugation by this enzyme is a major pathway for the biotransformation of phenolic and catechol drugs as well as neurotransmitters. EC 2.8.2.1.Carbohydrates: The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.Phosphoadenosine Phosphosulfate: 3'-Phosphoadenosine-5'-phosphosulfate. Key intermediate in the formation by living cells of sulfate esters of phenols, alcohols, steroids, sulfated polysaccharides, and simple esters, such as choline sulfate. It is formed from sulfate ion and ATP in a two-step process. This compound also is an important step in the process of sulfur fixation in plants and microorganisms.Corneal Dystrophies, Hereditary: Bilateral hereditary disorders of the cornea, usually autosomal dominant, which may be present at birth but more frequently develop during adolescence and progress slowly throughout life. Central macular dystrophy is transmitted as an autosomal recessive defect.Carbohydrate Metabolism: Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.Mannose-Binding Lectins: A subclass of lectins that are specific for CARBOHYDRATES that contain MANNOSE.Mannose: A hexose or fermentable monosaccharide and isomer of glucose from manna, the ash Fraxinus ornus and related plants. (From Grant & Hackh's Chemical Dictionary, 5th ed & Random House Unabridged Dictionary, 2d ed)Chondro-4-Sulfatase: An enzyme from the sulfuric ester hydrolase class that breaks down one of the products of the chondroitin lyase II reaction. EC 3.1.6.9.SulfatasesMucopolysaccharidoses: Group of lysosomal storage diseases each caused by an inherited deficiency of an enzyme involved in the degradation of glycosaminoglycans (mucopolysaccharides). The diseases are progressive and often display a wide spectrum of clinical severity within one enzyme deficiency.Steryl-Sulfatase: An arylsulfatase with high specificity towards sulfated steroids. Defects in this enzyme are the cause of ICHTHYOSIS, X-LINKED.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Physician-Patient Relations: The interactions between physician and patient.N-Acetylgalactosamine-4-Sulfatase: An arylsulfatase that catalyzes the hydrolysis of the 4-sulfate groups of the N-acetyl-D-galactosamine 4-sulfate units of chondroitin sulfate and dermatan sulfate. A deficiency of this enzyme is responsible for the inherited lysosomal disease, Maroteaux-Lamy syndrome (MUCOPOLYSACCHARIDOSIS VI). EC 3.1.6.12.Chondroitinsulfatases: A group of enzymes that catalyze the hydrolysis of various sulfate bonds of chondroitin sulfate. EC 3.1.6.-.Mucopolysaccharidosis IV: Genetic disorder of mucopolysaccharide metabolism characterized by skeletal abnormalities, joint instability, development of cervical myelopathy, and excessive urinary keratan sulfate. There are two biochemically distinct forms, each due to a deficiency of a different enzyme.Mucopolysaccharidosis VI: Mucopolysaccharidosis with excessive CHONDROITIN SULFATE B in urine, characterized by dwarfism and deafness. It is caused by a deficiency of N-ACETYLGALACTOSAMINE-4-SULFATASE (arylsulfatase B).Dictionaries, MedicalSan FranciscoChemical EngineeringTherapeutic Equivalency: The relative equivalency in the efficacy of different modes of treatment of a disease, most often used to compare the efficacy of different pharmaceuticals to treat a given disease.MassachusettsGenetic Engineering: Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.Protein Engineering: Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.Antibodies, Monoclonal, Humanized: Antibodies from non-human species whose protein sequences have been modified to make them nearly identical with human antibodies. If the constant region and part of the variable region are replaced, they are called humanized. If only the constant region is modified they are called chimeric. INN names for humanized antibodies end in -zumab.Ranunculaceae: The buttercup plant family of the order Ranunculales, subclass Magnoliidae, class Magnoliopsida. The leaves are usually alternate and stalkless. The flowers usually have two to five free sepals and may be radially symmetrical or irregular.Aconitum: A plant genus of the family RANUNCULACEAE. Members contain a number of diterpenoid alkaloids including: aconitans, hypaconitine, ACONITINE, jesaconitine, ignavine, napelline, and mesaconitine. The common name of Wolfbane is similar to the common name for ARNICA.Lectins: Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.Songbirds: PASSERIFORMES of the suborder, Oscines, in which the flexor tendons of the toes are separate, and the lower syrinx has 4 to 9 pairs of tensor muscles inserted at both ends of the tracheal half rings. They include many commonly recognized birds such as CROWS; FINCHES; robins; SPARROWS; and SWALLOWS.Plant Lectins: Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.Tetanus Toxin: Protein synthesized by CLOSTRIDIUM TETANI as a single chain of ~150 kDa with 35% sequence identity to BOTULINUM TOXIN that is cleaved to a light and a heavy chain that are linked by a single disulfide bond. Tetanolysin is the hemolytic and tetanospasmin is the neurotoxic principle. The toxin causes disruption of the inhibitory mechanisms of the CNS, thus permitting uncontrolled nervous activity, leading to fatal CONVULSIONS.Gangliosides: A subclass of ACIDIC GLYCOSPHINGOLIPIDS. They contain one or more sialic acid (N-ACETYLNEURAMINIC ACID) residues. Using the Svennerholm system of abbrevations, gangliosides are designated G for ganglioside, plus subscript M, D, or T for mono-, di-, or trisialo, respectively, the subscript letter being followed by a subscript arabic numeral to indicated sequence of migration in thin-layer chromatograms. (From Oxford Dictionary of Biochemistry and Molecular Biology, 1997)Botulinum Toxins: Toxic proteins produced from the species CLOSTRIDIUM BOTULINUM. The toxins are synthesized as a single peptide chain which is processed into a mature protein consisting of a heavy chain and light chain joined via a disulfide bond. The botulinum toxin light chain is a zinc-dependent protease which is released from the heavy chain upon ENDOCYTOSIS into PRESYNAPTIC NERVE ENDINGS. Once inside the cell the botulinum toxin light chain cleaves specific SNARE proteins which are essential for secretion of ACETYLCHOLINE by SYNAPTIC VESICLES. This inhibition of acetylcholine release results in muscular PARALYSIS.Tetanus: A disease caused by tetanospasmin, a powerful protein toxin produced by CLOSTRIDIUM TETANI. Tetanus usually occurs after an acute injury, such as a puncture wound or laceration. Generalized tetanus, the most common form, is characterized by tetanic muscular contractions and hyperreflexia. Localized tetanus presents itself as a mild condition with manifestations restricted to muscles near the wound. It may progress to the generalized form.G(M1) Ganglioside: A specific monosialoganglioside that accumulates abnormally within the nervous system due to a deficiency of GM1-b-galactosidase, resulting in GM1 gangliosidosis.G(M3) Ganglioside: A ganglioside present in abnormally large amounts in the brain and liver due to a deficient biosynthetic enzyme, G(M3):UDP-N-acetylgalactosaminyltransferase. Deficiency of this enzyme prevents the formation of G(M2) ganglioside from G(M3) ganglioside and is the cause of an anabolic sphingolipidosis.Tetanus ToxoidGlycosaminoglycans: Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.Disaccharides: Oligosaccharides containing two monosaccharide units linked by a glycosidic bond.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Iduronic Acid: Component of dermatan sulfate. Differs in configuration from glucuronic acid only at the C-5 position.Heparitin Sulfate: A heteropolysaccharide that is similar in structure to HEPARIN. It accumulates in individuals with MUCOPOLYSACCHARIDOSIS.Lubricants: Compounds that provide LUBRICATION between surfaces in order to reduce FRICTION.

Gangliosides of human kidney. (1/450)

Five gangliosides isolated from human kidney have been characterized. The two main fractions were shown to be typical extraneural gangliosides in having lactose as their neutral carbohydrate moiety. Their structures were identified as: AcNeu(alpha2-3)Gal(beta1-4)Glc(beta1-1)Cer and AcNeu(alpha2-8)AcNeu(alpha2-3)Gal(beta1-4)Glc(beta1-1)Cer. The two main hexosamine-containing gangliosides are structurally related to human blood group substances of glycosphingolipid nature. The following structures are postulated: AcNeu(alpha2-3)Gal(beta1-4)GlcNAc(beta1-3)Gal(beta1-4)Glc(beta1-1)Cer and AcNeu(alpha2-3)Gal(beta1-4)[Fuc(alpha1-3)]GlcNAc(beta1-3)Gal(beta1-4)Glc(beta1-1) Cer. The third hexosamine-containing ganglioside belongs to a different series of glycolipids and was shown to have the structure of a major ganglioside of human brain: AcNeu(alpha2-3)Gal(beta1-3)GalNAc(beta1-4)[AcNeu(alpha2-3)]Gal(beta1-4)Glc(beta1- 1)Cer. The fatty acid structure of different gangliosides was shown to resemble that of neutral glycolipids of human kidney with the nonhydroxy acids C16:0, C22:0, and C24:0 as major components.  (+info)

Binding partners for the myelin-associated glycoprotein of N2A neuroblastoma cells. (2/450)

The myelin-associated glycoprotein (MAG) has been proposed to be important for the integrity of myelinated axons. For a better understanding of the interactions involved in the binding of MAG to neuronal axons, we performed this study to identify the binding partners for MAG on neuronal cells. Experiments with glycosylation inhibitors revealed that sialylated N-glycans of glycoproteins represent the major binding sites for MAG on the neuroblastoma cell line N2A. From extracts of [3H]glucosamine-labelled N2A cells several glycoproteins with molecular weights between 20 and 230 kDa were affinity-precipitated using immobilised MAG. The interactions of these proteins with MAG were sialic acid-dependent and specific for MAG.  (+info)

Lipopolysaccharides (LPS) of oral black-pigmented bacteria induce tumor necrosis factor production by LPS-refractory C3H/HeJ macrophages in a way different from that of Salmonella LPS. (3/450)

Some lipopolysaccharide (LPS) preparations from S- or R-form members of the family Enterobacteriaceae and oral black-pigmented bacteria (Porphyromonas gingivalis and Prevotella intermedia) are known to activate LPS-refractory C3H/HeJ macrophages. When contaminating proteins are removed from R-form LPS of Enterobacteriaceae by repurification, however, this ability is lost. In the present study, we investigated the capacity of LPS from P. gingivalis, P. intermedia, Salmonella minnesota, and Salmonella abortusequi to induce production of tumor necrosis factor (TNF) in gamma interferon-primed C3H/HeJ macrophages before and after repurification. P. abortusequi S-LPS was fractionated by centrifugal partition chromatography into two LPS forms: SL-LPS, having homologous long O-polysaccharide chains, and SS-LPS having short oligosaccharide chains. Prior to repurification, all LPS forms except SL-LPS induced TNF production in both C3H/HeJ and C3H/HeN macrophages. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that repurification removed contaminating protein from the preparations, and repurified SS-LPS and S. minnesota Ra-LPS no longer stimulated TNF production in C3H/HeJ macrophages, although C3H/HeN macrophages remained responsive. In contrast, repurified oral bacterial LPS retained the capacity to induce TNF production in C3H/HeJ macrophages. Oral bacterial LPS preparations also were not antagonized by excess inactive, repurified SL-LPS; Ra-LPS; Rhodobacter sphaeroides lipid A, a competitive LPS antagonist, or paclitaxel, an LPS agonist, and they were comparatively resistant to polymyxin B treatment. Nevertheless, oral bacterial LPS was less toxic to D-galactosamine-treated C3H/HeN mice than was LPS from Salmonella. These findings indicate that the active molecule(s) and mode of action of LPS from P. gingivalis and P. intermedia are quite different from those of LPS from Salmonella.  (+info)

Dynamic epigenetic regulation of initial O-glycosylation by UDP-N-Acetylgalactosamine:Peptide N-acetylgalactosaminyltransferases. site-specific glycosylation of MUC1 repeat peptide influences the substrate qualities at adjacent or distant Ser/Thr positions. (4/450)

In search of possible epigenetic regulatory mechanisms ruling the initiation of O-glycosylation by polypeptide:N-acetylgalactosaminyltransferases, we studied the influences of mono- and disaccharide substituents of glycopeptide substrates on the site-specific in vitro addition of N-acetylgalactosamine (GalNAc) residues by recombinant GalNAc-Ts (rGalNAc-T1, -T2, and -T3). The substrates were 20-mers (HGV20) or 21-mers (AHG21) of the MUC1 tandem repeat peptide carrying GalNAcalpha or Galbeta1-3GalNAcalpha at different positions. The enzymatic products were analyzed by MALDI mass spectrometry and Edman degradation for the number and sites of incorporated GalNAc. Disaccharide placed on the first position of the diad Ser-16-Thr-17 prevents glycosylation of the second, whereas disaccharide on the second position of Ser-16-Thr-17 and Thr-5-Ser-6 does not prevent GalNAc addition to the first. Multiple disaccharide substituents suppress any further glycosylation at the remaining sites. Glycosylation of Ser-16 is negatively affected by glycosylation at position -6 (Thr-10) or -10 (Ser-6) and is inhibited by disaccharide at position -11 (Thr-5), suggesting the occurrence of glycosylation-induced effects on distant acceptor sites. Kinetic studies revealed the accelerated addition of GalNAc to Ser-16 adjacent to GalNAc-substituted Thr-17, demonstrating positive regulatory effects induced by glycosylation on the monosaccharide level. These antagonistic effects of mono- and disaccharides could underlie a postulated regulatory mechanism.  (+info)

Structural basis for the resistance of Tay-Sachs ganglioside GM2 to enzymatic degradation. (5/450)

To understand the reason why, in the absence of GM2 activator protein, the GalNAc and the NeuAc in GM2 (GalNAcbeta1-->4(NeuAcalpha2-->3)Galbeta1-->4Glcbet a1-1'Cer) are refractory to beta-hexosaminidase A and sialidase, respectively, we have recently synthesized a linkage analogue of GM2 named 6'GM2 (GalNAcbeta1-->6(NeuAcalpha2-->3)Galbeta1-->4Glcbet a1-1'Cer). While GM2 has GalNAcbeta1-->4Gal linkage, 6'-GM2 has GalNAcbeta1-->6Gal linkage (Ishida, H., Ito, Y., Tanahashi, E., Li, Y.-T., Kiso, M., and Hasegawa, A. (1997) Carbohydr. Res. 302, 223-227). We have studied the enzymatic susceptibilities of GM2 and 6'GM2, as well as that of the oligosaccharides derived from GM2, asialo-GM2 (GalNAcbeta1-->4Galbeta1--> 4Glcbeta1-1'Cer) and 6'GM2. In addition, the conformational properties of both GM2 and 6'GM2 were analyzed using NMR spectroscopy and molecular mechanics computation. In sharp contrast to GM2, the GalNAc and the Neu5Ac of 6'GM2 were readily hydrolyzed by beta-hexosaminidase A and sialidase, respectively, without GM2 activator. Among the oligosaccharides derived from GM2, asialo-GM2, and 6'GM2, only the oligosaccharide from GM2 was resistant to beta-hexosaminidase A. Conformational analyses revealed that while GM2 has a compact and rigid oligosaccharide head group, 6'GM2 has an open spatial arrangement of the sugar units, with the GalNAc and the Neu5Ac freely accessible to external interactions. These results strongly indicate that the resistance of GM2 to enzymatic hydrolysis is because of the specific rigid conformation of the GM2 oligosaccharide.  (+info)

The distribution of sugar chains on the vomeronasal epithelium observed with an atomic force microscope. (6/450)

The distribution of sugar chains on tissue sections of the rat vomeronasal epithelium, and the adhesive force between the sugar and its specific lectin were examined with an atomic force microscope (AFM). AFM tips were modified with a lectin, Vicia villosa agglutinin, which recognizes terminal N-acetyl-D-galactosamine (GalNAc). When a modified tip scanned the luminal surface of the sensory epithelium, adhesive interactions between the tip and the sample surface were observed. The final rupture force was calculated to be approximately 50 pN based on the spring constant of the AFM cantilever. Distribution patterns of sugar chains obtained from the force mapping image were very similar to those observed using fluorescence-labeled lectin staining. AFM also revealed distribution patterns of sugar chains at a higher resolution than those obtained with fluorescence microscopy. Most of the adhesive interactions disappeared when the scanning solution contained 1 mM GaINAc. The adhesive interactions were restored by removing the sugar from the solution. Findings suggest that the adhesion force observed are related to the binding force between the lectin and the sugars distributed across the vomeronasal epithelium.  (+info)

Interaction of human macrophage C-type lectin with O-linked N-acetylgalactosamine residues on mucin glycopeptides. (7/450)

A fluorescein-labeled synthetic peptide, PTTTPITTTTK, was converted into O-glycosylated glycopeptides with various numbers of attached N-acetyl-D-galactosamines (GalNAcs) by in vitro glycosylation with UDP-GalNAc and a microsomal fraction of LS174T human colon carcinoma cells. Glycopeptides with 1, 3, 5, and 6 GalNAc residues (G1, G3, G5, and G6) were obtained, and their sizes were confirmed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Their sequences were determined by a peptide sequencer to be PTTTGalNAcPITTTTK for G1, PTGalNAcTTPITGalNAcTGalNAcTTK for G3, PTTGalNAcTGalNAcPITGalNAcTGalNAcTGalNAcTK for G5, and PTGalNAcTGalNAcTGalNAcPITGalNAcTGalNAcTGalNAcTK for G6. A calcium-type human macrophage lectin (HML) was prepared in a recombinant form, and its interaction with these glycopeptides was investigated by surface plasmon resonance (SPR) spectroscopy and fluorescence polarization. The affinity of recombinant HML (rHML) for immobilized glycopeptides increased, as revealed by SPR, in parallel with the number of GalNAc. The highest affinity was obtained when the G6-peptide was immobilized at high density. Fluorescence polarization equilibrium-binding assays also revealed that the affinity of rHML for soluble gly-copeptides increased, depending on the number of attached GalNAcs. Carbohydrate recognition domain (CRD) fragments of HML were prepared, and their affinity for these four glycopeptides was also determined, this affinity was apparently lower than that of rHML. Affinity constants of rHML for the G3- and G5-peptides were 11- and 38-fold higher, respectively, than for the G1-peptide, whereas those of CRD fragments were only 2- and 6-fold higher, respectively. A chemical cross-linking study revealed that rHML but not recombinant CRD forms trimers in an aqueous solution. Thus, preferential binding of densely glycosylated O-linked glycopeptides should be due to the trimer formation of rHML.  (+info)

Selective killing of CD8+ cells with a 'memory' phenotype (CD62Llo) by the N-acetyl-D-galactosamine-specific lectin from Viscum album L. (8/450)

As reported previously by our group, among the toxic proteins from Viscum album L. only the mistletoe lectins (MLs) induce the apoptotic killing pathway in human lymphocytes. Although one may expect a homogenous distribution of carbohydrate domains on cell surface receptors for the carbohydrate binding B chains of the toxic protein, the sensitivity of cells to these B chains obviously differ. Here we report a selective killing of CD8+ CD62Llo cells from healthy individuals by the galNAc-specific ML III (and RCA60, which binds to gal and galNAc), while the gal-specific ML I was less effective. This selective killing is not sufficiently explained by protein synthesis inhibition alone, since this subset was not affected by other ribosome inhibiting proteins such as the lectin from Ricinus communis (RCA120), lectin from Abrus precatorus (APA), abrin A, and inhibitors of RNA, DNA and/or protein synthesis such as actinomycin D, mitomycin C, and cycloheximide. We conclude that CD8+ cells with 'memory' phenotype (CD62Llo) are more sensitive to the ML III-mediated killing than their CD8+ CD62Lhi counterparts, CD4+ T cells, and CD19+ B cells. These cells probably express a distinct receptor with galNAc domains that is missing or not active on CD8+ cells with a 'naive' phenotype.  (+info)

Rat monoclonal Macrophage specific lectin antibody [ER-MP23]. Validated in IHC, Flow Cyt and tested in Mouse. Cited in 6 publication(s). Independently reviewed in 1 review(s). Immunogen corresponding…
2LMS: A single N-acetylgalactosamine residue at threonine 106 modifies the dynamics and structure of interferon alpha2a around the glycosylation site.
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
Lipid rafts, sterol- and sphingolipid-rich membrane microdomains, have been shown to control virulence in a variety of parasites including Entamoeba histolytica, an intestinal parasite that causes dysentery and liver abscess. Parasite cell surface receptors, such as the Gal/GalNAc lectin, facilitate attachment to host cells and extracellular matrix. The Gal/GalNAc lectin binds to galactose or N-acetylgalactosamine residues on host components, and is composed of heavy (Hgl), intermediate (Igl), and light (Lgl) subunits. Although Igl is constitutively localized to lipid rafts, Hgl and Lgl transiently associate with this compartment in a cholesterol-dependent fashion. Exposure to bonafide Gal/GalNAc lectin ligands is associated with enrichment of the subunits in rafts. Direct lectin-ligand interactions and sufficient levels of both PIP2 and calcium were shown to be necessary for lectin enrichment in rafts. Additionally, an initial analysis of both post-translational modifications and protein interactions
[button size=small text=MSDS & Datasheet link=/wp-content/uploads/media/BCDatasheets_C_10.26/BXXXX/B-3601-2.pdf] Anti-A Helix pomatia Lectin (Edib
Dermatan 4-sulfotransferase (EC 2.8.2.35, dermatan-specific N-acetylgalactosamine 4-O-sulfotransferase, dermatan-4-sulfotransferase-1, dermatan-4-sulfotransferase 1, D4ST-1, dermatan N-acetylgalactosamine 4-O-sulfotransferase, CHST14 protein, CHST14) is an enzyme with systematic name 3-phospho-5-adenylyl sulfate:(dermatan)-N-acetyl-D-galactosamine 4-sulfotransferase. This enzyme catalyses the following chemical reaction 3-phospho-5-adenylyl sulfate + [dermatan]-N-acetyl-D-galactosamine ⇌ {\displaystyle \rightleftharpoons } adenosine 3,5-bisphosphate + [dermatan]-4-O-sulfo-N-acetyl-D-galactosamine The sulfation takes place at the 4-position of N-acetyl-D-galactosamine residues of dermatan. Evers, M.R.; Xia, G.; Kang, H.G.; Schachner, M.; Baenziger, J.U. (2001). "Molecular cloning and characterization of a dermatan-specific N-acetylgalactosamine 4-O-sulfotransferase". J. Biol. Chem. 276: 36344-36353. doi:10.1074/jbc.M105848200. PMID 11470797. Mikami, T.; Mizumoto, S.; Kago, N.; Kitagawa, ...
Styphnolobium japonicum agglutinin (SJA) is affinity purified from Japanese pagoda tree seeds. It consists of two subunits. The subunits can be separated into two subfractions, a D-galactose/N-acetyl-D-galactosamine specific lectin (B-SJA-I) and a D-mannose/D-glucose specific lectin (B-SJA-II). SJA has an isoelectric p
B4GALNT2 catalyzes the last step in the biosynthesis of the human Sd(a) antigen through the addition of an N-acetylgalactosamine residue via a beta-1,4 linkage to a subterminal galactose residue substituted with an alpha-2,3-linked sialic acid. B4GALNT2 also catalyzes the last step in the biosynthesis of the Cad antigen (Montiel et al., 2003 [PubMed 12678917]).[supplied by OMIM, Mar 2008 ...
They fused the sequence for Bt toxin Cry1Ac with that of the nontoxic B-chain subunit of ricin (RB) in a recombinant plasmid. RB is a leptin that binds with galactose as well as N-acetylgalactosamine residues with high affinity, the latter of which are key components of Bt toxin-binding receptors. Then embryonic callus from mature maize seeds were bombarded with this BtRB fusion. The researchers tested their fusion toxin against stem borer Chilo suppressalis, a pest normally susceptible to Cry1Ac, and found that maize producing low levels of BtRB killed 75 per cent of larvae, compared with 17 per cent in Bt-only plants. Similar trials with the cotton leaf worm Spodoptera littoralis, which is resistant to Bt delta endotoxins, showed that after 4 days, nearly 78 per cent of larvae died on BtRB maize, compared with less than 20 per cent on Bt-only or nontransformed maize. In the leafhopper Cicadulina mbila, which like other homopterans was ordinarily not affected by Bt toxins, 95 per cent of ...
View mouse Chst15 Chr7:132235780-132317228 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
Was the shower scene presented in a titillating or gratuitous manner? Not a rhetorical question; I genuinely dont know, since I havent seen it. Remind me agai
Looking for online definition of carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14 in the Medical Dictionary? carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14 explanation free. What is carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14? Meaning of carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14 medical term. What does carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14 mean?
Aberrant glycosylation occurs in essentially all types of human cancers. A difference in glycopattern of proteins will result in a change of function of the proteins. The lectin from Helix pomatia (HPA) recognizes N-acetylgalactosaminylated glycoproteins and very consistent results over the increased binding of HPA in tissue sections are associated with metastasis progression and poor patient prognosis in a range of human adenocarcinomas. The induced modification of protein function after changed glycosylation is unknown, and as a part in characterizing the glycoproteins carrying the specific carbohydrates, we analyzed the major HPA binding proteins in sera from healthy women, women with primary breast cancer with no metastasis (bcmet-), and women with metastasizing breast cancer (bcmet+) using lectin affinity chromatography and lectin blotting. The binding ligands were further identified using mass spectrometry (MALDI-TOF MS) to confirm the captured glycoproteins. The major HPA binding proteins ...
Sie sind hier: Glycopolymer Brushes for Specific Lectin Binding by Controlled Multivalent Presentation of N-acetyllactosamine Glycan Oligomers. ...
A purified 4-O-sulfatase has been identified, characterized, and produced, which is able to selectively hydrolyze sulfate groups (desulfation) at position 4 of N-acetylgalactosamine residues, substituted or not (e.g. chondroitin-4-sulfate). This specific position is a sulfation site that is not targeted by currently used (at lab-scale) enzymes. (2) - This recombinant enzyme can be used in synthesis methods of oligo- and polysaccharides, including GAGs. GAGs are usually extracted from a wide range of agricultural resources, and are used as food additives (to adjust texture, viscosity, fineness, etc.) - The second application of this enzyme family, coming from their high specificity, is as diagnostic or sequencing tools for the characterization of GAGs of interest, and thus for a better understanding of their properties. Indeed, determining the type of sulfation of an oligo- or polysaccharide may be performed by testing desulfation with this 4-O-sulfatase in combination with methods of ...
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the contacts provided below. For general information, Learn About Clinical Studies. ...
St6galnac6 (untagged) - Mouse ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 (St6galnac6), transcript variant 2, (10ug), 10 µg.
ST6GALNAC4 antibody [N2C3] (ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 4) for WB. Anti-ST6GALNAC4 pAb (GTX119976) is tested in Human samples. 100% Ab-Assurance.
... , Authors: Jean-Loup Huret, Philippe Dessen. Published in: Atlas Genet Cytogenet Oncol Haematol.
A lectin was isolated from root tubers of winter aconite (Eranthis hyemalis) by affinity chromatography on fetuin-agarose, and it was partially characterized with respect to its biochemical, physicochemical and carbohydrate-binding properties. The Eranthis hyemalis lectin is a dimeric protein (Mr 62000) composed of two different subunits of Mr 30000 and 32000, held together by disulphide bonds. It is especially rich in asparagine/aspartic acid, glutamine/glutamic acid and leucine, and contains 5% covalently bound carbohydrate. Hapten inhibition assays indicated that the winter-aconite lectin is specific for N-acetylgalactosamine. In addition, the lectin exhibits a pronounced specificity towards blood-group-O erythrocytes. The winter-aconite lectin is the first lectin to be isolated from a species belonging to the plant family Ranunculaceae. It appears to be different from all previously described plant lectins.. ...
In the field of biochemistry, O-linked glycosylation is the attachment of a sugar molecule to an oxygen atom in an amino acid residue in a protein. O-linked glycosylation is a form of glycosylation that occurs in the Golgi apparatus in eukaryotes. It also occurs in archaea and bacteria. O-linked glycosylation occurs at a later stage during protein processing, probably in the Golgi apparatus. This is the addition of N-acetyl-galactosamine to serine or threonine residues by the enzyme UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (EC number 2.4.1.41), followed by other carbohydrates (such as galactose and sialic acid). This process is important for certain types of proteins such as proteoglycans, which involves the addition of glycosaminoglycan chains to an initially unglycosylated "proteoglycan core protein." These additions are usually serine O-linked glycoproteins, which seem to have one of two main functions. One function involves secretion to form components of ...
Definition of vicia villosa in the Definitions.net dictionary. Meaning of vicia villosa. What does vicia villosa mean? Information and translations of vicia villosa in the most comprehensive dictionary definitions resource on the web.
Abnormal O-glycans expressed by cancer cells have functional importance in cell adhesion, invasion, and metastasis [15]. Alterations in mucin-type O-glycans has been associated with malignant transformation, resulting in the formation of less complex structures and leading to an increase of the simple short determinants. Protein O-glycosylation is deregulated in breast cancer cells, leading to the accumulation of simple mucin-type tumor-associated antigens [37]. The expression of GalNAc-T14 mRNA was analyzed in normal and malignant tissue from breast, skin, lung, pancreas, ovary, endometrium, bladder and lymphoid cancers. A subset of tumor samples, ranging from 10% in lobular breast cancer to 30% in lung cancer and diffuse large B-cell lymphoma, showed GalNAc-T14 mRNA overexpression [36]. Under thees circumstances, we hypothesize the expression of GalNAc-T14 may be a useful biomarker for breast cancer by immunohistochemistry.. It has been shown that several glycosyltransferases are useful tumor ...
2004;25(3):237-72. This virus is particularly important for recognizing antigen alone, the treated animals made dependent again display more severe when the ng aspirate are found in some myeloproliferative disorders may be caused by slow filling of the most effective avan-5-ol is epicatechin gallate, epigallocatechin gallate, epigallocatechin, gallocatechin, epicatechin, and gallocatechin gallate. De groot, j. C and lester, h. A. And fletcher, c. (1983), antigenic variation in host responsesit is important to note that dopamine d1 receptors (farde, halldin, stone-elander, sedvall, 1984 gaspar, bloch, le moine, c. (1993). Rev infect dis, 1987. 604 617). Could possibly cause crystalluria and hematuria. Journal of pharmacology committee on quality of phytodrugs varies widely: Perfect extraction, titration, and association areas differs from our own. Hemoperitoneum, due to inhibition of the gal/galnac-specific lectin suggests that the frontal lobe more than 4 weeks, whereas cholestatic or mixed ...
Polyagglutination refers to red blood cells that agglutinate upon exposure to almost all human sera, but not to autologous serum or the sera of newborns. The condition becomes apparent during blood typing and cross-matching in the laboratory (summary by {1:Beck, 2000}). Tn polyagglutination syndrome is an acquired clonal disorder characterized by the polyagglutination of red blood cells by naturally occurring anti-Tn antibodies following exposure of the Tn antigen on the surface of erythrocytes. Only a subset of red cells express the antigen, which can also be expressed on platelets and leukocytes. This condition may occur in healthy individuals who manifest asymptomatic anemia, leukopenia, or thrombocytopenia; however, there is also an association between the Tn antigen and leukemia or myelodysplastic disorders. The Tn antigen is an incompletely glycosylated membrane glycoprotein with an exposed N-acetylgalactosamine residue. The Tn antigen results from inactivation of C1GALT1C1, which encodes ...
A lectin present in the seeds of the osage orange has been purified and has a specificity for galactose and N-acetyl-D-galactosamine. The lectin can be resolved into five different isolectins 1 . Like Jacalin, the lectin has an α 4 β 4 structure that shows far greater micro-heterogeniety than plant lectin from other families, due to multiple genetic isoforms and posttranslational processing 2 . The subunits have been sequenced 2,3 and are highly homologous to Jacalin. In the presence of a ligand, Galβ1,3GalNAc (T-antigen disaccharide), an isoform of the lectin appears to crystallize as a dimer 4 . The nominal carbohydrate specificity of MPA is similar to that of GS-I. However, while GS-I is a blood group B specific lectin, MPA reacts most strongly with type O and A cells. Also, while neuraminidase treatment of the erythrocytes increases their reactivity with MPA, GS-I is only slightly more reactive with treated cells. Purified MPA is a very stable lectin, maintaining activity when heated to ...
Get this from a library! Cell surface carbohydrate chemistry. [Robert E Harmon; American Chemical Society. Division of Carbohydrate Chemistry.;]
Hello, I have a live Helix pomatia snails for sale. Price $ 4.50 for one snail. If you buy 10 or more - price would be $ 4 for each one. We proudly ship
1FIH: Mechanism of pH-dependent N-acetylgalactosamine binding by a functional mimic of the hepatocyte asialoglycoprotein receptor.
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Dolichos biflorus|/em| agglutinin is a glycoprotein with a molecular weight of about 111 kDa and consists of 4 subunits of approximately equal size. This lectin has a carbohydrate specificity toward α-linked N-acetylgalactosamine.
Author Summary Gene conversion is a process of recombination that can generate diversity among genes. Gene conversion occurs in some pathogenic species of protozoa to generate diversity among gene families encoding important antigens. The process may contribute to immune evasion by the parasites. Gene conversion, or indeed recombination of any kind, has not previously been demonstrated in human intestinal parasites of the genus Entamoeba. Here, we analysed genes encoding members of an important antigenic protein complex on the surface of Entamoeba parasites which is involved in invasion of the intestinal wall. Three gene families encode heavy-, light- and intermediate-subunits of the complex. We estimated genetic divergence between related genes from two species of Entamoeba, E. histolytica and E. dispar, and compared them to divergence among neighbouring genes and to the average across the whole genome, initially looking for evidence that the genes were evolving under positive selection. However,
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FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Glycosylation of proteins affects cell-cell interaction, interactions with the matrix, and the functions of intracellular molecules. ST6GALNAC1 transfers a sialic acid, N-acetylneuraminic acid (NeuAc), in an alpha-2,6 linkage to O-linked GalNAc residues. The cancer-associated sialyl-Tn (sTn) antigen is formed by ST6GALNAC1-catalyzed sialylation of GalNAc residues on mucins (Ikehara et al., 1999 [PubMed 10536037]; Sewell et al., 2006 [PubMed 16319059]).[supplied by OMIM, Mar 2008 ...
This lectin is a family of tetrameric glycoproteins consisting of combinations of A and B subunits similar in structure to PHA and GSL I.
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β-Glucuronidase, Helix pomatia Native β-glucuronidase from Helix pomatia. Useful for hydrolyzing urinary steroid conjugates prior to steriod assays. Note: 1 MU = 1,000,000 units. - Find MSDS or SDS, a COA, data sheets and more information.
IMPFSTOFFE + VAKZINE (PHARMAZIE); OBERFLÄCHENANTIGENE (IMMUNOLOGIE); ORGANISCHE SYNTHESE (CHEMIE); PROTEIN-MIKROARRAY-TECHNOLOGIE (MOLEKULARGENETISCHE TECHNIKEN); VACCINES (PHARMACY); SURFACE ANTIGENS (IMMUNOLOGY); ORGANIC SYNTHESIS (CHEMISTRY); PROTEIN MICROARRAY TECHNOLOGY (MOLECULAR GENETIC TECHNIQUES ...
α-1,3-galactosyltransferase (EC 2.4.1.87); α-1,3 N-acetylgalactosaminyltransferase (EC 2.4.1.40); α-galactosyltransferase (EC 2.4.1.37); globoside α-N-acetylgalactosaminyltransferase (EC 2.4.1.88 ...
α-1,3-galactosyltransferase (EC 2.4.1.87); α-1,3 N-acetylgalactosaminyltransferase (EC 2.4.1.40); α-galactosyltransferase (EC 2.4.1.37); globoside α-N-acetylgalactosaminyltransferase (EC 2.4.1.88 ...
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Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Sodium atom in PDB 3ecq: Endo-Alpha-N-Acetylgalactosaminidase From Streptococcus Pneumoniae: Semet Structure
Glycosaminoglycans (GAGs) are linear polysaccharide chains consisting of repeating disaccharide units and form proteglycans by covalently attaching to their core proteins. Chondroitin sulfate (CS) is a glycosaminoglycan with the disaccharide unit GalNAc(b1-4)GlcA(b1-3), modified with ester-linked sulfate at certain positions. Dermatan sulfate (DS) is a modified form of CS, in which a portion of D-glucuronate residues is epimerized to L-iduronates. CS and DS are linked to serine residues in core proteins via a linkage tetrasaccharide formed by the transfer of xylose and three more residues [MD:M00057]. The assembly process of CS is initiated by the transfer of N-acetylgalactosamine to the linkage tetrasaccharide. The polymerization step is catalyzed by bifunctional enzymes (chondroitin synthases) that have both b1-3 glucuronosyltransferase and b1-4 N-acetylgalactosaminyltransferase activities [MD:M00058]. Chondroitin polymerization also requires the action of the chondroitin polymerizing factor. ...
The ability of a peptide vaccine to stimulate T cells in vivo might be improved by specifically targeting the peptide to dendritic cells (DC). The C-type lectin Macrophage Galactose-type lectin, MGL (CD301), has been shown to bind to N-acetyl-galactosamine (GalNAc) and small peptides bearing O-linked GalNAc. Synthetic GalNAc can be produced using relatively simple organic chemistry when compared with the complicated branched sugars that are recognised by other C-type lectins. MGL therefore represents a promising target for the design of synthetic peptide vaccines. We have identified that antigen-presenting cells in human skin express MGL and have confirmed that CD14+ dermal DCs might be targeted via MGL. The intracellular fate of MGL following internalisation was tracked by confocal microscopy. MGL traffics through early endosomes to late endosomes/lysosomes, and colocalises with MHC class I and class II. Synthetic glycopeptides were produced incorporating either native O-linked GalNAc or GalNAc ...
The methyl glycosides of N-acetyl-d-glucosamine (d-GlcNAc) and N-acetyl-d-galactosamine (d-GalNAc) have been used as model glycan analogs to study the effects of lithium cation binding on glycan structure in gas-phase experiments. Infrared multiple photon dissociation (IRMPD) spectra for the two Li+-complexed anomers of methyl-d-GlcNAc revealed a difference of 10 cm−1 between their respective carbonyl stretching band positions. A corresponding 11 cm−1 shift was observed for the two Li+-complexed anomers of methyl-d-GalNAc. Theoretical calculations indicate that the position of the methyl group (α and β, or axial and equatorial, respectively) on carbon 1 of the sugar and its close proximity to the carbonyl of the acetamido group on carbon 2 cause the average orientation of the carbonyl to change in order to minimize steric hindrance. This change in orientation is postulated to be the cause of the observed Cdouble bond; length as m-dashO stretching band shift. The calculations also predict ...
Franziska Beran, Sujit Adhikary, Sankar Gayen, Christian Ulrichs, Arunava Goswami: Genetic Polymorphism of Dolichos biflorus L. in India at the Seed Storage Level
Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of dermatan sulfate. Plays a pivotal role in the formation of 4-0-sulfated IdoA blocks in dermatan sulfate. Transfers sulfate to the C-4 hydroxyl of beta1,4-linked GalNAc that is substituted with an alpha-linked iduronic acid (IdoUA) at the C-3 hydroxyl. Transfers sulfate more efficiently to GalNAc residues in -IdoUA-GalNAc-IdoUA- than in -GlcUA-GalNAc-GlcUA-sequences. Has preference for partially desulfated dermatan sulfate. Addition of sulfate to GalNAc may occur immediately after epimerization of GlcUA to IdoUA. Appears to have an important role in the formation of the cerebellar neural network during postnatal brain development. ...
Accepted name: α-N-acetylgalactosaminide α-2,6-sialyltransferase Reaction: CMP-N-acetylneuraminate + glycano-(1→3)-(N-acetyl-α-D-galactosaminyl)-glycoprotein = CMP + glycano-[(2→6)-α-N-acetylneuraminyl]-(N-acetyl-D-galactosaminyl)-glycoprotein. Systematic name: CMP-N-acetylneuraminate:glycano-1,3-(N-acetyl-α-D-galactosaminyl)-glycoprotein α-2,6-N-acetylneuraminyltransferase Comments: α-N-Acetylgalactosamine linked to threonine or serine is also an acceptor, when substituted at the 3-position. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: 71124-50-0. References: 1. Sadler, J.E., Rearick, J.I. and Hill, R.L. Purification to homogeneity and enzymatic characterization of an α-N-acetylgalactosaminide α2→6 sialyltransferase from porcine submaxillary glands. J. Biol. Chem. 254 (1979) 5934-5941. [PMID: 447688] ...
Christensen B, et al. (2005) Post-translationally modified residues of native human osteopontin are located in clusters: identification of 36 phosphorylation and five O-glycosylation sites and their biological implications. Biochem J 390, 285-92 ...
Expression of ST6GALNAC2 (SIAT7, SIAT7B, SIATL1, ST6GalNAII, STHM) in oral mucosa tissue. Antibody staining with HPA048849 in immunohistochemistry.
The Viennese people have a long tradition of preparing and consuming escargot (Helix pomatia), going back to the Middle Ages. For the Romans in Carnuntum
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Входит в надсемейство белков лектины C-типа/лектино-подобный домен C-типа (CTL/CTLD). Белки этого надсемейства имеют похожую конформацию и обладают различными функциями, включая клеточную адгезию, межклеточную передачу сигнала, обмен гликопротеинов, участие в воспалении и иммунном ответе. CLL-1 является отрицательным регулятором гранулоцитарных и моноцитарных функций. ...
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This unit describes release of oligosaccharides that are attached to polypeptides through an N-acetylgalactosamine (GalNAc) linkage to the hydroxyl groups of serine or threonine. The b-elimination procedures described here can be used to recover the oligosaccharide chains (also called glycans) and/or identify the serine or threonine residues involved in the linkage. A b-elimination method employing sodium borohydride (NaBH4) and alkaline conditions is described, and an alternative method is also presented in which only alkaline conditions are used without a reducing agent. Another alternative protocol uses sodium sulfite. ...
Asialoglycoprotein Receptor 1兔多克隆抗体(ab42488)可与大鼠, 人样本反应并经WB, ICC/IF实验严格验证,被2篇文献引用并得到2个独立的用户反馈。
Carbohydrate-oligonucleotide conjugates have become increasingly important in recent years, particularly with regard to an increased uptake and cell availability of siRNA and antisense oligonucleotides. Of particular interest here is N-acetylgalactosamine (GalNAc), which can bind to the asialoglycoprotein receptor (ASGPR) in hepatocytes.. The following picture shows some examples of the attachment of carbohydrates (sugars) to oligonucleotides via different linker structures:. ...
Carbohydrate-oligonucleotide conjugates have become increasingly important in recent years, particularly with regard to an increased uptake and cell availability of siRNA and antisense oligonucleotides. Of particular interest here is N-acetylgalactosamine (GalNAc), which can bind to the asialoglycoprotein receptor (ASGPR) in hepatocytes.. The following picture shows some examples of the attachment of carbohydrates (sugars) to oligonucleotides via different linker structures:. ...
Understanding the way lectins work is paramount to understanding the positive impact the Blood Type Diet can have on you. But before exploring how lectins behave within your body, you have to understand what a lectin actually is. In the simplest of terms, a lectin is a type of protein that acts as a selective Velcro-like material. They come in two main forms, single and double-sided. The single sided lectins only stick to other things. Cells in the liver have this type of lectin on their surfaces to snatch up harmful bacteria and parasites that may be present. Two sided lectins stick two other cells together, like a piece of double-sided tape between objects. Both varieties, despite their differences, have many commonalities. Although both the single and double sided kinds both cause agglutination (the clumping of particles), they do so in specific, individualized ways. Each lectin has a certain type of material it is meant to bind to and this changes depending on what species the
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Bäckström, M., Link, T., Olson, F. J., Karlsson, H., Graham, R., Picco, G., Burchell, J., Taylor-Papadimitriou, J., and Noll, T. (2003). Recombinant MUC1 with breast cancer-like O-glycosylation produced in large amounts in CHO-K1 cells. Biochemical Journal 376, 677-686 ...
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Expression of ST6GALNAC2 (SIAT7, SIAT7B, SIATL1, ST6GalNAII, STHM) in esophagus tissue. Antibody staining with HPA048849 in immunohistochemistry.
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Foregut duplication cysts are developmental anomalies of the bronchopulmonary foregut and are common cystic lesions of the mediastinum. We describe a case of mediastinal foregut duplication cyst with in vivo H-1 MR spectroscopy on a 1.5T magnet showing a large metabolite peak at 2.02 ppm, attributable to N-acetylated compounds, in addition to a smaller peak at 1.33 ppm, considered to represent lipids. In vitro NMR spectroscopy (7.05T) of cyst fluid confirmed the presence of these peaks. In addition; a broad multiplet centered at 3.7 ppm, possibly from various protons of the hexose ring system, was also noted. Chemical analysis of the cyst fluid demonstrated the presence of N-acetylhexosamines, proteins, and lipids: Again, in vitro spectra of pure samples of N-acetylglucosamine and N-acetylgalactosamine were obtained for comparison, which better resolved the N-acetyl peak and the peaks at 3.7 ppm. The mucus secreted by respiratory epithelium and the mucous glands of the foregut cysts contains ...
Seeds of Triticale (hybrid of wheat and rye) contain an N-acetylglucosamine specific lectin that was affinity purified in our laboratory (Siva Kumar, N. and Padma, K. (1996) Affinity purification of N-acetyl glucosamine specific lectin. Purification and partial characterization of Triticale lectin. Biochem. Mol. Biol. Int. 38, 1059-1066). Seed extracts also exhibited alpha-mannosidase activity that was isolated by a combination of ion exchange, hydrophobic chromatography and gel filtration. The purified enzyme is a glycoprotein with 7% carbohydrate and exhibited a native molecular mass of 1,95,000 (+/-5000) on Biogel P-200 and dissociated into two major subunits under reducing conditions of molecular masses 58 and 40 kDa, respectively. Both subunits cross-reacted with an antibody to the well-characterized jack bean alpha-mannosidase, suggesting antigenic similarity between the legume and the cereal mannosidases. Purified enzyme binds to Con A-Sepharose gel, possibly through the sugar-binding site.
Authors: Ju, Tongzhong , Aryal, Rajindra P. , Kudelka, Matthew R. , Wang, Yingchun , Cummings, Richard D. Article Type: Research Article Abstract: The Tn antigen is a tumor-associated carbohydrate antigen that is not normally expressed in peripheral tissues or blood cells. Expression of this antigen, which is found in a majority of human carcinomas of all types, arises from a blockage in the normal O-glycosylation pathway in which glycans are extended from the common precursor GalNAcα1-O-Ser/Thr (Tn antigen). This precursor is generated in the Golgi apparatus on newly synthesized glycoproteins by a family of polypeptide α-N-acetylgalactosaminyltransferases (ppGalNAcTs) and then extended to the common core 1 O-glycan Galβ1-3GalNAcα1-O-Ser/Thr (T antigen) by a single enzyme termed the T-synthase (core 1 β3-galactosyltransferase or C1GalT). Formation …of the active form of the T-synthase requires a unique molecular chaperone termed Cosmc, encoded by Cosmc on the X-chromosome (Xq24 in humans, ...
This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. The encoded protein is capable of glycosylating fibronectin peptide in vitro and is expressed in a fibroblast cell line, indicating that it may be involved in the synthesis of oncofetal fibronectin ...
I have a recombinant protein produced in the yeast Pichia pastoris that is partially glycosylated with O-linked sugar (it must be O-linked - there are no N-linked sites). I wish to remove the glycosylated protein from the non-glycosylated. I have tried using ConA-Sepharose (Pharmacia) and Glycine-Max (Sigma). Neither of these has appeared to bind. The ConA comes with instructions that have been followed. No instructions could be found for Gycine-MAx and Sigma were not forthcoming. Has anyone has experience with trying to purify O-linked sugars, with either of the above lectins, or using any other reagents, I would very much like to hear from you. Please reply to me by e-mail as I do not regularly read this newsgroup. Tim -- Tim Dudgeon British Biotech Phone: 0865 748747 FAX: 0865 717598 email: dudgeon at britbio.co.uk ...
Predicted to have N-acetylgalactosamine 4-O-sulfotransferase activity. Predicted to be involved in dermatan sulfate biosynthetic process. Predicted to localize to the Golgi apparatus and integral component of membrane. Human ortholog(s) of this gene implicated in Ehlers-Danlos syndrome. Is expressed in several structures, including brain; liver; and retina. Orthologous to human CHST14 (carbohydrate sulfotransferase 14 ...
Ting, C; Nunn, N E.; Park, J Y.; and Herberman, R B., "Comparison of three isotopic assays of cell-mediated cytotoxicity against mouse tumor cells. II. Sensitivity and specificity of the assays and characteristics of effector and sensitizing cells." (1977). Subject Strain Bibliography 1977. 1932 ...
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You can, I think, get most of these either by shape or name. Ek, alm, ask, are so close, and hassel...The famous escargot, Helix pomatia. Also known as Roman, Burgundy, or simply edible snail. Or, when in the Rome of the north, Snäckor. Introduced, running rampant in a slick way.Yeah, stickmygga.Damn it! Found on a…
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The number of Asialoglycoprotein (ASGP) receptors on the hepatocytes of patients with liver disease is reduced and is thus considered a good indicator for the evaluation of liver function. ASGP receptor-targeted radiopharmaceuticals permit a non-invasive way to evaluate total and regional hepatic function and hepatic functional reserve visually and quantitatively. Over the past three decades, a variety of ASGP receptor-targeted probes have been developed with different molecular backbones (albumin, polymer, small-molecular-weight ligand), different glycol-residues (galactose, lactose, N-acetyl-galactosamine) and different chelating systems suitable for radiolabeling with SPECT isotopes (Tc-99m, In-111, Ga-67, (131)/I-125, Sm-153) and PET isotopes (Ga-68, F-18). In this review, we present an overview of ASGP receptor-targeted radiopharmaceuticals, discuss their chemistry, biodistribution, catabolism and challenge as well as application for measurement of liver function ...
Since the 1980s, Israeli virologist Madeleine Mumcuoglu, PhD, of the Hadassah-Hebrew University Medical Center in Jerusalem has studied the antiviral properties of elderberry. In laboratory research, she discovered compounds in elderberry that bind to spikes on the surface of virus cells, preventing them from puncturing cell membranes. The high concentrations of flavonoids in elderberry inhibit the action of neuraminidase, the enzyme that helps the flu virus attach to and penetrate new cells. Mumcuoglu tested Sambucol, the proprietary elderberry extract she helped develop, against various strains of influenza A and B in the laboratory and found the herb effective against all types of flu.. In 1993, a flu epidemic at an Israeli kibbutz provided the opportunity to test elderberry on patients. In people with full-blown flu symptoms, half of the subjects were given 4 tablespoons of standardized elderberry extract daily and the other half were given a placebo. Within 24 hours, 20 percent of the ...
The protein encoded by this gene belongs to the sulfotransferase 2 family. It is localized to the golgi membrane, and catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage, and is distributed on the surfaces of many cells and extracellular matrices. Alternatively spliced transcript variants differing only in their 5 UTRs have been found for this gene.
Pneumococcal lipoteichoic acid (LTA) is known to have a completely different chemical structure compared with that of Staphylococcus aureus: the polyglycerophosphate in the backbone is replaced in the pneumococcal LTA by a pentamer repeating unit consisting of one ribitol and a tetrasaccharide carrying the unusual substituents phosphocholine andN-acetyl-D-galactosamine. NeitherD-alaninenorN-acetyl D-glucosamine, which play central roles in the biological activity of the staphylococcal LTA, has been reported. The extraction using butanol is more gentle compared with the previously reported chloroform-methanol extraction and results in a higher yield of LTA. We characterized the LTA of two different strains of Streptococcus pneumoniae: R6 (serotype 2) and Fp23 (serotype 4). NMR analysis confirmed the structure of LTA from R6 but showed that its ribitol carries an N-acetyl-D-galactosamine substituent. The NMRdata for the LTA from Fp23 indicate that this LTA additionally contains ribitolbound ...
OPN (osteopontin) is a multiphosphorylated extracellular glycoprotein, which has important roles in bone remodelling, inflammation and cancer metastasis. OPN regulates cell spreading and adhesion primarily through its association with several integrins such as αvβ3, and its phosphorylation affects these processes. However, the mechanism by which OPN O-glycosylation affects these processes is not completely understood. In the present study, we demonstrated that OPN O-glycosylation self-regulates its biological activities and also affects its phosphorylation status. We prepared two recombinant OPNs, WT (wild-type)-OPN and mutant OPN (ΔO-OPN), which lacks five O-glycosylation sites at a threonine/proline-rich region. O-glycan defects in OPN increased its phosphorylation level, as observed by dephosphorylation assays. Moreover, compared with WT-OPN, ΔO-OPN exhibited enhanced cell spreading and adhesion activities and decreased associations with β1 integrins. This suggested that defects in ...
Wisteria floribunda lectin (WFA/WFL). Wisteria floribunda agglutinin or lectin (WFA/WFL) is isolated from Japanese Wisteria seeds. It is a glycoprotein with a molecular weight...
Studies of mucins suggest that the structural effects of O-glycans are restricted to steric interactions between peptide-linked GalNAc residues and adjacent polypeptide residues. It has been proposed, however, that differential O-glycan sialylation alters the structure of the stalk-like region of the T cell co-receptor, CD8, and that this, in turn, modulates ligand binding (Daniels, M. A., Devine, L., Miller, J. D., Moser, J. M., Lukacher, A. E., Altman, J. D., Kavathas, P., Hogquist, K. A., and Jameson, S. C. (2001) Immunity 15, 1051-1061; Moody, A. M., Chui, D., Reche, P. A., Priatel, J. J., Marth, J. D., and Reinherz, E. L. (2001) Cell 107, 501-512). We characterize the glycosylation of soluble, chimeric forms of the alphaalpha- and alphabeta-isoforms of murine CD8 containing the O-glycosylated stalk of rat CD8alphaalpha, and we show that the stalk O-glycans are differentially sialylated in CHO K1 versus Lec3.2.8.1 cells (82 versus approximately 6%, respectively). Sedimentation analysis indicates
Best price in Australia on Now Foods Elderberry and Zinc - 30 Lozenges from eVitamins.com. Find Elderberry and Zinc reviews, side effects, coupons and more from eVitamins. Fast and reliable shipping to the Australia. Elderberry and Zinc and other products by Now Foods for all your health needs.
GSL II is affinity purified tetramer that contains a single type of 30 kDa subunit. It has insecticidal structure/function, the first GlcNAc binding legume lectin proven to have insecticidal activity.This lectin has a carbohydrate specificity for αGal and αGalNAc and elutes with galactose or N-acetylgalactosamine. Incr
During the winter, my family takes elderberry syrup along with other supplements to boost our immune systems. Elderberries have been known to shorten the duration of illness, and its a berry so its generally considered safe for pretty much anyone including pregnant and nursing women. I personally have taken it while pregnant and nursing. I really enjoy making and taking the elderberry syrup. If you dont want to make your own elderberry syrup, you can buy it here. Elderberries (sambucus nigra) grow in the wild, and you can harvest them yourself and make the syrup if you would like. Some of the benefits of elderberries are: cold ad flu relief, blood sugar stabilizer, natural diuretic, good for allergies, and high in antioxidants. They are also high in vitamins A, B6 and C, which helps them boost the immune system. I purchase my organic elderberries in bulk from Amazon. Theyve always been great quality and I like buying in bulk so I dont have to worry about running out when I want to make it. ...
Question 4. How can a group AB individual produce an anti-A1 antibody?. N-acetylgalactosamine (GalNAc) is added to H antigen to create the A antigen. The A1 phenotype results from the "wild-type" A101 allele, which produces an enzyme capable of converting approximately 1,000,000 H antigens into A antigens. However, the A2 phenotype results from a non-sense mutation in the A101 allele, such that the translated enzyme contains 21 extra amino acids and is hypofunctional compared to the wild type A-synthesizing enzyme (see Figure 1 below). Less A antigens are created on A2 cells and an anti-A1 can be produced. Thus an AB individual with an anti-A1 in their serum is most likely A2B.. ...
... skincare labelling is not very common but I think it is an important logo as it will usually mean that the product is also cruelty-free. However please also look for a cruelty-free logo because you cannot assume that the products ingredients or the end product itself, albeit vegetable-derived, has not been tested on animals. Never assume. Additionally, the ingredients listed on a skincare label does not make it easy to spot if an ingredient is vegetarian- or animal-based. For example "Helix pomatia" is becoming a popular ingredient in mainstream skincare products and has been used in Korean skincare products for many years because it is reported to have excellent moisturising, anti-wrinkle, anti-aging, and anti-oxidant properties. Sounds like a fabulous ingredient for your skin, doesnt it? But what if I told you that Helix pomatia is known as Snail serum, in laymans terms? And what if I told you that theres nothing beautiful or humane about breeding snails solely for ...
Elderberry Eases Inflammation .An abundant source of anthocyanins, black elderberry extract was examined for its possible to impact markers of swelling
Rabbit Polyclonal Anti-Asialoglycoprotein Receptor 2 Antibody. Validated: WB, IHC, IHC-P. Tested Reactivity: Human. 100% Guaranteed.
Elderberry, medically known as Sambucus nigra, is a fruit-bearing shrub native to Europe and North America. With a long history of use in folk medicine,...
A plant that always gets me to thinking with my stomach is common elderberry (Sambucus canadensis), which is just now coming into bloom along roadside...
... however this is much weaker than the inhibition seen with N-acetylgalactosamine (range 92-100%).[1] ... "Inhibition of elastase enzyme release from human polymorphonuclear leukocytes by N-acetyl-galactosamine and N-acetyl- ...
N-Acetylgalactosamine. References[edit]. This article needs additional citations for verification. Please help improve this ...
The repeating unit (except for keratan) consists of an amino sugar (N-acetylglucosamine or N-acetylgalactosamine) along with a ... GalNAc(4S,6S) = β-D-N-acetylgalactosamine-4-O, 6-O-sulfate ... GalNAc = β-D-N-acetylgalactosamine. *GalNAc(4S) = β-D-N- ... GalNAc(6S) = β-D-N-acetylgalactosamine-6-O-sulfate. * ...
GLASER L (1959). "The biosynthesis of N-acetylgalactosamine". J. Biol. Chem. 234: 2801-5. PMID 13828347. Kornfeld S; Glaser L ( ...
N-acetylgalactosamine-6-sulfatase is an enzyme that, in humans, is encoded by the GALNS gene. This gene encodes N- ... N-acetylgalactosamine-6-sulfate sulfatase exonic point mutations in classical Morquio and mild cases". J. Clin. Invest. 90 (3 ... Masue M, Sukegawa K, Orii T, Hashimoto T (1992). "N-acetylgalactosamine-6-sulfate sulfatase in human placenta: purification and ... Jan 1992). "Morquio disease: isolation, characterization and expression of full-length cDNA for human N-acetylgalactosamine-6- ...
This gene product mainly transfers sulfate to N-acetylgalactosamine. The regulated expression of each member of this gene ...
Baig, S., Wilcock, G., & Love, S. (2005). Loss of perineuronal net N-acetylgalactosamine in Alzheimer's disease. Acta ...
". "UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter [Homo sapien - Protein - NCBI]". www.ncbi.nlm.nih.gov. Retrieved ...
UDP-N-acetylgalactosamine: (N-acetylneuraminyl)-galactosylglucosyl ceramide N-acetylgalactosaminyltransferase in rat brain". ... Hashimoto, Y.; Sekine, M.; Iwasaki, K.; Suzuki, A. (1993). "Purification and characterization of UDP-N-acetylgalactosamine GM3/ ... UDP acetylgalactosamine-(N-acetylneuraminyl)-D-galactosyl-D-glucosylceramide acetylgalactosaminyltransferase, UDP-N-acetyl-D- ... UDP-N-acetylgalactosamine GM3 N-acetylgalactosaminyltransferase, uridine diphosphoacetylgalactosamine- ...
Aldurazyme by BioMarin Pharmaceutical and Genzyme N-acetylgalactosamine-4-sulfatase (rhASB; galsulfase): Naglazyme by BioMarin ...
"Molecular cloning and characterization of a dermatan-specific N-acetylgalactosamine 4-O-sulfotransferase". The Journal of ... "Specificities of three distinct human chondroitin/dermatan N-acetylgalactosamine 4-O-sulfotransferases demonstrated using ...
GSTs catalyze sulfation at the 6-hydroxyl group of galactose, N-acetylgalactosamine, or N-acetylglucosamine. Like heparan ... There are two major families of carbohydrate sulfotransferases: heparan sulfotransferases and galactose/N-acetylgalactosamine/N ... which transfer sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. ... "Molecular cloning and characterization of a dermatan-specific N-acetylgalactosamine 4-O-sulfotransferase". J. Biol. Chem. 276 ( ...
... (EC 2.8.2.35, dermatan-specific N-acetylgalactosamine 4-O-sulfotransferase, dermatan-4- ... "Molecular cloning and characterization of a dermatan-specific N-acetylgalactosamine 4-O-sulfotransferase". J. Biol. Chem. 276: ... "Specificities of three distinct human chondroitin/dermatan N-acetylgalactosamine 4-O-sulfotransferases demonstrated using ... sulfotransferase-1, dermatan-4-sulfotransferase 1, D4ST-1, dermatan N-acetylgalactosamine 4-O-sulfotransferase, CHST14 protein ...
The enzyme coded for by IA adds an N-acetylgalactosamine to the membrane-bound H antigen. The IB enzyme adds a galactose. The i ...
White square = N-acetyl-galactosamine; black circle = galactose; Black square = N-acetyl-glucosamine. Note: There is a mistake ... A Core 2 structure is generated by the addition of N-acetyl-glucosamine to the N-acetyl-galactosamine of the Core 1 structure. ... Core 3 structures are generated by the addition of a single N-acetyl-glucosamine to the original N-acetyl-galactosamine. Core 4 ... The first monosaccharide attached in the synthesis of O-linked glycans is N-acetyl-galactosamine. After this, several different ...
... (N-acetylgalactosamine-4-sulfatase, chondroitinsulfatase, chondroitinase, acetylgalactosamine 4-sulfatase, N- ... N-acetylgalactosamine-4-sulfatase): potential role as a biomarker in prostate cancer". Prostate Cancer and Prostatic Diseases. ... acetylgalactosamine 4-sulfate sulfohydrolase, EC 3.1.6.12) is an enzyme associated with mucopolysaccharidosis VI (Maroteaux- ...
The sugars are usually a combination of N-acetylgalactosamine, D-glucose or D-galactose. A glycosphingolipid that has only one ...
Taniguchi N, Makita A (1984). "Purification and characterization of UDP-N-acetylgalactosamine: globotriaosylceramide beta-3-N- ... Identification of beta 3Gal-T3 as UDP-N-acetylgalactosamine:globotriaosylceramide beta 1,3-N-acetylgalactosaminyltransferase". ... Identification of four inactivating mutations in the UDP-N-acetylgalactosamine: globotriaosylceramide 3-beta-N- ... N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding ...
Taniguchi N, Makita A (1984). "Purification and characterization of UDP-N-acetylgalactosamine: globotriaosylceramide beta-3-N- ... Identification of beta 3Gal-T3 as UDP-N-acetylgalactosamine:globotriaosylceramide beta 1,3-N-acetylgalactosaminyltransferase". ... UDP-N-acetylgalactosamine:globotriaosylceramide, beta1,3-N-acetylgalactosaminyltransferase, globoside synthase, ... UDP-N-acetylgalactosamine:globotriaosylceramide, beta-3-N-acetylgalactosaminyltransferase, galactosylgalactosylglucosylceramide ...
Ohtake S, Ito Y, Fukuta M, Habuchi O (Nov 2001). "Human N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase cDNA is related to ... "Difference between N-acetylgalactosamine 4-sulfate 6-O-sulfotransferases from human serum and squid cartilage in specificity ... "A unique nonreducing terminal modification of chondroitin sulfate by N-acetylgalactosamine 4-sulfate 6-o-sulfotransferase". J. ... D-galactopyranoside is a competitive acceptor that decreases sulfation of chondroitin sulfate by N-acetylgalactosamine 4- ...
The first sugar is always N-acetyl-galactosamine (GalNAc), followed by other galactoses and sialic acid. In HSP and IgAN, these ...
The galactose component originates from UDP-galactose and the GalNAc component originates from UDP-N-acetylgalactosamine. These ... is an exopolysaccharide composed of galactose and N-acetylgalactosamine (GalNAc). It is commonly found in the biofilm and cell ...
UDP-N-acetylgalactosamine:polypeptide, N-acetylgalactosaminyltransferase, and UDP-N-acetylgalactosamine:protein N- ... UDP-N-acetylgalactosamine-glycoprotein, N-acetylgalactosaminyltransferase, UDP-N-acetylgalactosamine-protein N- ... polypeptide-N-acetylgalactosamine transferase, UDP-acetylgalactosamine-glycoprotein, acetylgalactosaminyltransferase, UDP- ... Takeuchi M, Yoshikawa M, Sasaki R, Chiba H (1985). "Purification and characterization of UDP-N-acetylgalactosamine-kappa-casein ...
Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase Amado M, Almeida R, Schwientek T, Clausen H (December 1999). " ... Core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1, also known as C1GALT1, is an enzyme which ...
... characterization of a novel chondroitin sulfate glucuronyltransferase that transfers glucuronic acid to N-acetylgalactosamine ...
... PubChem Notes: Acetylgalactosamine The N-acetyl derivative of galactosamine. ACETYLGALACTOSAMINE Galactose, 2-(acetylamino)-2-deoxy- N-[(3R,4R,5R,6R)-2,4,5-trihydroxy-6-(hydroxymethyl)oxan-3-yl]acetamide ...
N-Acetylgalactosamine (GalNAc), is an amino sugar derivative of galactose. In humans it is the terminal carbohydrate forming ... N-Acetylgalactosamine is necessary for intercellular communication, and is concentrated in sensory nerve structures of both ... Destruction of Blood Group A Activity by an Enzyme from Clostridium tertium Which Deacetylates N-Acetylgalactosamine in Intact ...
In enzymology, a N-acetylgalactosamine kinase (EC 2.7.1.157) is an enzyme that catalyzes the chemical reaction ATP + N-acetyl-D ... Other names in common use include GALK2, GK2, GalNAc kinase, and N-acetylgalactosamine (GalNAc)-1-phosphate kinase. Pastuszak I ... Thoden JB, Holden HM (2005). "The molecular architecture of human N-acetyl galactosamine kinase". J. Biol. Chem. 280 (38): ... Drake R, Elbein AD (1996). "Kidney N-acetylgalactosamine (GalNAc)-1-phosphate kinase, a new pathway of GalNAc activation". J. ...
N-acetylgalactosamine-6-sulfate sulfatase, and N-acetylgalactosamine 6-sulfatase. This enzyme participates in glycosaminoglycan ... In enzymology, a N-acetylgalactosamine-6-sulfatase (EC 3.1.6.4) is an enzyme that catalyzes the chemical reaction of cleaving ... Lim CT, Horwitz AL (1981). "Purification and properties of human N-acetylgalactosamine-6-sulfate sulfatase". Biochim. Biophys. ... Other names in common use include chondroitin sulfatase, chondroitinase, galactose-6-sulfate sulfatase, acetylgalactosamine 6- ...
N-acetylgalactosamine-4-sulfatase (EC 3.1.6.12, chondroitinsulfatase, chondroitinase, arylsulfatase B, acetylgalactosamine 4- ... N-acetylgalactosamine-4-sulfatase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and Cellular ... Tsuji, M.; Nakanishi, Y.; Habuchi, H.; Ishihara, K.; Suzuki, S. (1980). "The common identity of UDP-N-acetylgalactosamine 4- ... sulfatase, N-acetylgalactosamine 4-sulfate sulfohydrolase) is an enzyme with systematic name N-acetyl-D-galactosamine-4-sulfate ...
UDP-N-acetylgalactosamine diphosphorylase (EC 2.7.7.83) is an enzyme with systematic name UTP:N-acetyl-alpha-D-galactosamine-1- ... UDP-N-acetylgalactosamine diphosphorylase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and ...
The X-ray structure of the N-acetylgalactosamine deacetylase reveals the active site and mechanism of the founding member of an ... Crystal structure of an N-acetylgalactosamine deacetylase from F. plautii. *DOI: 10.2210/pdb6N1A/pdb ...
In enzymology, a glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase (EC 2.4.1.122) is an enzyme that catalyzes the ...
In enzymology, an UDP-N-acetylgalactosamine-4-sulfate sulfotransferase (EC 2.8.2.7) is an enzyme that catalyzes the chemical ... uridine diphospho-N-acetylgalactosamine 4-sulfate sulfotransferase, and uridine diphosphoacetylgalactosamine 4-sulfate ... phosphosulfate to uridine diphosphate N-acetylgalactosamine 4-sulfate". J. Biol. Chem. 242 (9): 2288-90. PMID 6022874. ...
N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase (EC 2.8.2.33, GalNAc4S-6ST, CHST15 (gene)) is an enzyme with systematic ... N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase at the US National Library of Medicine Medical Subject Headings (MeSH) ... Ohtake, S.; Ito, Y.; Fukuta, M.; Habuchi, O. (2001). "Human N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase cDNA is ... Ito, Y.; Habuchi, O. (2000). "Purification and characterization of N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase from ...
N Acetylgalactosamine 4 sulfate Sulfatase. An arylsulfatase that catalyzes the Hydrolysis of the 4-sulfate groups of the N- ...
Antibodies for proteins involved in N-acetylgalactosamine kinase activity pathways, according to their Panther/Gene Ontology ... Antibodies for proteins involved in N-acetylgalactosamine kinase activity pathways; according to their Panther/Gene Ontology ...
Mucopolysaccharidosis IVA is an autosomal recessive disease caused by the deficiency of N-acetylgalactosamine-6-sulfate ... Effect of attenuated mutations in mucopolysaccharidosis IVA on molecular phenotypes of N-acetylgalactosamine-6-sulfate ...
... we demonstrated production of an active recombinant human N-acetylgalactosamine-6-sulfatase (rhGALNS) enzyme in Escherichia ... Recombinant human N-acetylgalactosamine-6-sulfate sulfatase (GALNS) produced in the methylotrophic yeast Pichia pastoris. Sci ... Improvement in the production of the human recombinant enzyme N-acetylgalactosamine-6-sulfatase (rhGALNS) in Escherichia coli ... Characterization of a recombinant N-acetylgalactosamine-6-sulfate sulfatase produced in E. coli for enzyme replacement therapy ...
Mechanism of pH-dependent N-acetylgalactosamine binding by a functional mimic of the hepatocyte asialoglycoprotein receptor. ... N-ACETYLGALACTOSAMINE-SELECTIVE MUTANT OF MANNOSE-BINDING PROTEIN-A (QPDWG-HDRPY). *DOI: 10.2210/pdb1fif/pdb ...
A peptide mimetic of a ligand for the galactose/N-acetylgalactosamine-specific C-type lectin receptors (GCLR) exhibited ... Monocyte galactose/N-acetylgalactosamine-specific C-type lectin receptor stimulant immunotherapy of an experimental glioma. ... Monocyte galactose/N-acetylgalactosamine-specific C-type lectin receptor stimulant immunotherapy of an experimental glioma. ... Background: A peptide mimetic of a ligand for the galactose/N-acetylgalactosamine-specific C-type lectin receptors (GCLR) ...
Rabbit Polyclonal Anti-N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody. Validated: WB, ICC/IF, IHC, IHC-P. Tested Reactivity: ... Additional N-Acetylgalactosamine-6-Sulfatase/GALNS Products. N-Acetylgalactosamine-6-Sulfatase/GALNS NBP1-32899 * N- ... Blogs on N-Acetylgalactosamine-6-Sulfatase/GALNS. There are no specific blogs for N-Acetylgalactosamine-6-Sulfatase/GALNS, but ... PTMs for N-Acetylgalactosamine-6-Sulfatase/GALNS Antibody (NBP1-32899). Learn more about PTMs related to N-Acetylgalactosamine- ...
N-acetylgalactosamine-N,N-diacetylbacillosaminyl-diphospho-undecaprenol 4-alpha-N-acetylgalactosaminyltransferase at the US ... N-acetylgalactosamine-N, N-diacetylbacillosaminyl-diphospho-undecaprenol 4-alpha-N-acetylgalactosaminyltransferase (EC 2.4. ...
View mouse Chst15 Chr7:132235780-132317228 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
S. Ohtake-Niimi, S. Kondo, T. Ito et al., "Mice deficient in N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase are unable to ... S. Ohtake, Y. Ito, M. Fukuta, and O. Habuchi, "Human N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase cDNA is related to ... Chondroitin sulfate (CS) containing E-disaccharide units, glucuronic acid-N-acetylgalactosamine(4, 6-O-disulfate), at surfaces ... Expression of N-Acetylgalactosamine 4-Sulfate 6-O-Sulfotransferase Involved in Chondroitin Sulfate Synthesis Is Responsible for ...
This staining demonstrates glycoconjugate with terminal N-acetylgalactosamine (GalNAc) in periodic foci on the neuronal surface ... Postnatal appearance of glycoconjugate with terminal N-acetylgalactosamine on the surface of selected neurons in mouse brain ... This staining demonstrates glycoconjugate with terminal N-acetylgalactosamine (GalNAc) in periodic foci on the neuronal surface ...
Mechanism of pH-dependent N-acetylgalactosamine binding by a functional mimic of the hepatocyte asialoglycoprotein receptor. ... N-ACETYLGALACTOSAMINE BINDING MUTANT OF MANNOSE-BINDING PROTEIN A (QPDWG-HDRPY), COMPLEX WITH N-ACETYLGALACTOSAMINE. *DOI: ...
Open-Label Study of Efficacy and Safety of Recombinant Human N-acetylgalactosamine 4-sulfatase in Patients With MPS VI. The ... Open-Label Study of Efficacy and Safety of Recombinant Human N-acetylgalactosamine 4-sulfatase in Patients With MPS VI. ... and pharmacokinetics of weekly intravenous infusions of 1 mg/kg recombinant human N-acetylgalactosamine 4-sulfatase (rhASB) in ...
... Lookup it up at Rhymes.net - the most comprehensive rhyming words ... Weve got 0 rhyming words for n-acetylgalactosamine-4-sulfatase ». What rhymes with n-acetylgalactosamine-4-sulfatase?. This ... Search for Song lyrics containing the word n-acetylgalactosamine-4-sulfatase. *Search for n-acetylgalactosamine-4-sulfatase on ... Know what rhymes with n-acetylgalactosamine-4-sulfatase? Have another rhyming word for n-acetylgalactosamine-4-sulfatase? Let ...
Plasma and Liver Protein Binding of N-Acetylgalactosamine-Conjugated Small Interfering RNA. Sara C. Humphreys, Mai B. Thayer, ... Plasma and Liver Protein Binding of N-Acetylgalactosamine-Conjugated Small Interfering RNA ...
  • In this study, to clarify the role of E-units in the metastasis and to search for potential molecular targets for anticancer drugs, the isolation and characterization of Lewis lung carcinoma (LLC) cells stably downregulated by the knockdown for the gene encoding N -acetylgalactosamine 4- O -sulfate 6- O -sulfotransferase (GalNAc4S-6ST), which is responsible for the formation of E-units in CS chains, were performed. (hindawi.com)
  • Functional conservation of subfamilies of putative UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyltransferases in Drosophila, Caenorhabditis elegans, and mammals. (wikipedia.org)
  • The sequon is an Asn-X-Ser or Asn-X-Thr sequence, where X is any amino acid except proline and the glycan may be composed of N-acetylgalactosamine, galactose, neuraminic acid, N-acetylglucosamine, fucose, mannose, and other monosaccharides. (wikipedia.org)
  • The sugar portion of the hormone is covalently bonded to asparagine, and is composed of N-acetylgalactosamine, mannose, N-acetylglucosamine, galactose, and sialic acid. (wikipedia.org)
  • The ABO gene locus expressing the glycosyltransferases has three main allelic forms: A, B, and O. The A allele encodes 1-3-N-acetylgalactosaminyltransferase that bonds α-N-acetylgalactosamine to D-galactose end of H antigen, producing the A antigen. (wikipedia.org)
  • Monocyte galactose/N-acetylgalactosamine-specific C-type lectin receptor stimulant immunotherapy of an experimental glioma. (dovepress.com)
  • A peptide mimetic of a ligand for the galactose/N-acetylgalactosamine-specific C-type lectin receptors (GCLR) exhibited monocyte-stimulating activity, but did not extend survival when applied alone against a syngeneic murine malignant glioma. (dovepress.com)
  • Hapten inhibition assays indicated that the winter-aconite lectin is specific for N-acetylgalactosamine. (biochemj.org)
  • N-acetylhexosamine 1-kinase (EC 2.7.1.162, NahK, LnpB, N-acetylgalactosamine/N-acetylglucosamine 1-kinase) is an enzyme with systematic name ATP:N-acetyl-D-hexosamine 1-phosphotransferase. (wikipedia.org)
  • The tumor suppressor EXT-like gene EXTL2 encodes an α1,4-N-acetylhexosaminyltransferase that transfers N-acetylgalactosamine and N-acetylglucosamine to the common glycosaminoglycan-protein linkage region. (wikipedia.org)
  • Acetylgalactosamine The N-acetyl derivative of galactosamine. (scitoys.com)
  • UDP-N-acetylgalactosamine diphosphorylase (EC 2.7.7.83) is an enzyme with systematic name UTP:N-acetyl-alpha-D-galactosamine-1-phosphate uridylyltransferase. (wikipedia.org)
  • N-acetylgalactosamine-N, N'-diacetylbacillosaminyl-diphospho-undecaprenol 4-alpha-N-acetylgalactosaminyltransferase (EC 2.4.1.291, PglJ) is an enzyme with systematic name UDP-N-acetyl-alpha-D-galactosamine:N-acetylgalactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol 3-alpha-N-acetyl-D-galactosaminyltransferase. (wikipedia.org)
  • This enzyme catalyses the following chemical reaction UDP-N-acetyl-D-galactosamine + globotriosylceramide ⇌ {\displaystyle \rightleftharpoons } UDP + globotetraosylceramide The product has a terminal beta-N-acetylgalactosamine residue. (wikipedia.org)
  • EhLINE1 encode an endonuclease (EN) protein (in addition to reverse transcriptase and nucleotide-binding ORF1), which have similarity with bacterial restriction endonuclease. (wikipedia.org)
  • UDP-N-Acetylgalactosamine is an activated sugar, and can be used for in vitro glycoengineering of glycoproteins, e.g . therapeutic proteins. (roche.com)
  • Identification of N-Acetylgalactosamine-containing glycoproteins PEB3 and CgpA in Campylobacter jejuni. (kingston.ac.uk)
  • Linton, D. , Allan, E. , Karlyshev, A.V. and Wren, B.W. (2001) Identification of N-Acetylgalactosamine-containing glycoproteins PEB3 and CgpA in Campylobacter jejuni. (kingston.ac.uk)
  • Each GAG chain consists of a linear pattern of alternating monosaccharide units: uronic acid and either N-acetylglucosamine or N-acetylgalactosamine. (wikipedia.org)