Acetylesterase: An enzyme that catalyzes the conversion of acetate esters and water to alcohols and acetate. EC 3.1.1.6.EsterasesInfluenzavirus C: A genus of the family ORTHOMYXOVIRIDAE comprising viruses similar to types A and B but less common, more stable, more homogeneous, and lacking the neuraminidase protein. They have not been associated with epidemics but may cause mild influenza. Influenza C virus is the type species.Triacetin: A triglyceride that is used as an antifungal agent.Shigella flexneri: A bacterium which is one of the etiologic agents of bacillary dysentery (DYSENTERY, BACILLARY) and sometimes of infantile gastroenteritis.Triolein: (Z)-9-Octadecenoic acid 1,2,3-propanetriyl ester.Shigella: A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that ferments sugar without gas production. Its organisms are intestinal pathogens of man and other primates and cause bacillary dysentery (DYSENTERY, BACILLARY).beta-Galactosidase: A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.Bacteroidetes: A phylum of bacteria comprised of three classes: Bacteroides, Flavobacteria, and Sphingobacteria.Forsythia: A plant genus of the family OLEACEAE. Members contain suspensaside.Sialic Acids: A group of naturally occurring N-and O-acyl derivatives of the deoxyamino sugar neuraminic acid. They are ubiquitously distributed in many tissues.Treponema denticola: A species of bacteria in the family SPIROCHAETACEAE, frequently isolated from periodontal pockets (PERIODONTAL POCKET).N-Acetylneuraminic Acid: An N-acyl derivative of neuraminic acid. N-acetylneuraminic acid occurs in many polysaccharides, glycoproteins, and glycolipids in animals and bacteria. (From Dorland, 28th ed, p1518)Bacteroides: A genus of gram-negative, anaerobic, rod-shaped bacteria. Its organisms are normal inhabitants of the oral, respiratory, intestinal, and urogenital cavities of humans, animals, and insects. Some species may be pathogenic.Carboxylic Ester Hydrolases: Enzymes which catalyze the hydrolysis of carboxylic acid esters with the formation of an alcohol and a carboxylic acid anion.Plant Mucilage: A type of viscous polysaccharide that is secreted from PLANTS. It has natural properties that are useful in the formulation of ADHESIVES.Pectins: High molecular weight polysaccharides present in the cell walls of all plants. Pectins cement cell walls together. They are used as emulsifiers and stabilizers in the food industry. They have been tried for a variety of therapeutic uses including as antidiarrheals, where they are now generally considered ineffective, and in the treatment of hypercholesterolemia.Plants, Genetically Modified: PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.Epoxide Hydrolases: Enzymes that catalyze reversibly the formation of an epoxide or arene oxide from a glycol or aromatic diol, respectively.Hydrolysis: The process of cleaving a chemical compound by the addition of a molecule of water.Human Body: The human being as a non-anatomical and non-zoological entity. The emphasis is on the philosophical or artistic treatment of the human being, and includes lay and social attitudes toward the body in history. (From J. Cassedy, NLM History of Medicine Division)Naphthol AS D Esterase: Hydrolytic enzyme activity used as a histocytochemical test for the presence of esterases in tissue. Substrate used is 3-hydroxy-4'-nitro-2-naphthanilide chloroacetate (naphthol AS-D).Sterol Esterase: An enzyme that catalyzes the hydrolysis of CHOLESTEROL ESTERS and some other sterol esters, to liberate cholesterol plus a fatty acid anion.Cellulase: An endocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages in CELLULOSE, lichenin, and cereal beta-glucans.Cellulases: A family of glycosidases that hydrolyse crystalline CELLULOSE into soluble sugar molecules. Within this family there are a variety of enzyme subtypes with differing substrate specificities that must work together to bring about complete cellulose hydrolysis. They are found in structures called CELLULOSOMES.Cellulose: A polysaccharide with glucose units linked as in CELLOBIOSE. It is the chief constituent of plant fibers, cotton being the purest natural form of the substance. As a raw material, it forms the basis for many derivatives used in chromatography, ion exchange materials, explosives manufacturing, and pharmaceutical preparations.Neurospora crassa: A species of ascomycetous fungi of the family Sordariaceae, order SORDARIALES, much used in biochemical, genetic, and physiologic studies.Neurospora: A genus of ascomycetous fungi, family Sordariaceae, order SORDARIALES, comprising bread molds. They are capable of converting tryptophan to nicotinic acid and are used extensively in genetic and enzyme research. (Dorland, 27th ed)Receptor-Like Protein Tyrosine Phosphatases: A subcategory of protein tyrosine phosphatases that are bound to the cell membrane. They contain cytoplasmic tyrosine phosphatase domains and extracellular protein domains that may play a role in cell-cell interactions by interacting with EXTRACELLULAR MATRIX components. They are considered receptor-like proteins in that they appear to lack specific ligands.Biofuels: Hydrocarbon-rich byproducts from the non-fossilized BIOMASS that are combusted to generate energy as opposed to fossilized hydrocarbon deposits (FOSSIL FUELS).Allergy and Immunology: A medical specialty concerned with the hypersensitivity of the individual to foreign substances and protection from the resultant infection or disorder.Access to Information: Individual's rights to obtain and use information collected or generated by others.Immunotherapy: Manipulation of the host's immune system in treatment of disease. It includes both active and passive immunization as well as immunosuppressive therapy to prevent graft rejection.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Journal Impact Factor: A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.Gangliosides: A subclass of ACIDIC GLYCOSPHINGOLIPIDS. They contain one or more sialic acid (N-ACETYLNEURAMINIC ACID) residues. Using the Svennerholm system of abbrevations, gangliosides are designated G for ganglioside, plus subscript M, D, or T for mono-, di-, or trisialo, respectively, the subscript letter being followed by a subscript arabic numeral to indicated sequence of migration in thin-layer chromatograms. (From Oxford Dictionary of Biochemistry and Molecular Biology, 1997)Immune System Diseases: Disorders caused by abnormal or absent immunologic mechanisms, whether humoral, cell-mediated, or both.Brenner Tumor: A smooth, solid or cystic fibroepithelial (FIBROEPITHELIAL NEOPLASMS) tumor, usually found in the OVARIES but can also be found in the adnexal region and the KIDNEYS. It consists of a fibrous stroma with nests of epithelial cells that sometimes resemble the transitional cells lining the urinary bladder. Brenner tumors generally are benign and asymptomatic. Malignant Brenner tumors have been reported.Authorship: The profession of writing. Also the identity of the writer as the creator of a literary production.Carcinoma, Renal Cell: A heterogeneous group of sporadic or hereditary carcinoma derived from cells of the KIDNEYS. There are several subtypes including the clear cells, the papillary, the chromophobe, the collecting duct, the spindle cells (sarcomatoid), or mixed cell-type carcinoma.Pancreatic Neoplasms: Tumors or cancer of the PANCREAS. Depending on the types of ISLET CELLS present in the tumors, various hormones can be secreted: GLUCAGON from PANCREATIC ALPHA CELLS; INSULIN from PANCREATIC BETA CELLS; and SOMATOSTATIN from the SOMATOSTATIN-SECRETING CELLS. Most are malignant except the insulin-producing tumors (INSULINOMA).Atracurium: A non-depolarizing neuromuscular blocking agent with short duration of action. Its lack of significant cardiovascular effects and its lack of dependence on good kidney function for elimination provide clinical advantage over alternate non-depolarizing neuromuscular blocking agents.Carcinoma, Pancreatic Ductal: Carcinoma that arises from the PANCREATIC DUCTS. It accounts for the majority of cancers derived from the PANCREAS.Kidney Neoplasms: Tumors or cancers of the KIDNEY.Alginates: Salts of alginic acid that are extracted from marine kelp and used to make dental impressions and as absorbent material for surgical dressings.Hexuronic Acids: Term used to designate tetrahydroxy aldehydic acids obtained by oxidation of hexose sugars, i.e. glucuronic acid, galacturonic acid, etc. Historically, the name hexuronic acid was originally given to ascorbic acid.Glucuronic Acid: A sugar acid formed by the oxidation of the C-6 carbon of GLUCOSE. In addition to being a key intermediate metabolite of the uronic acid pathway, glucuronic acid also plays a role in the detoxification of certain drugs and toxins by conjugating with them to form GLUCURONIDES.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Software: Sequential operating programs and data which instruct the functioning of a digital computer.

Purification and characterization of an acetyl xylan esterase from Bacillus pumilus. (1/152)

Bacillus pumilus PS213 was found to be able to release acetate from acetylated xylan. The enzyme catalyzing this reaction has been purified to homogeneity and characterized. The enzyme was secreted, and its production was induced by corncob powder and xylan. Its molecular mass, as determined by gel filtration, is 190 kDa, while sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed a single band of 40 kDa. The isoelectric point was found to be 4.8, and the enzyme activity was optimal at 55 degrees C and pH 8.0. The activity was inhibited by most of the metal ions, while no enhancement was observed. The Michaelis contant (Km) and Vmax for alpha-naphthyl acetate were 1.54 mM and 360 micromol min-1 mg of protein-1, respectively.  (+info)

The hemagglutinin-esterase of mouse hepatitis virus strain S is a sialate-4-O-acetylesterase. (2/152)

By comparative analysis of the hemagglutinin-esterase (HE) protein of mouse hepatitis virus strain S (MHV-S) and the HE protein of influenza C virus, we found major differences in substrate specificities. In striking contrast to the influenza C virus enzyme, the MHV-S esterase was unable to release acetate from bovine submandibulary gland mucin. Furthermore, MHV-S could not remove influenza C virus receptors from erythrocytes. Analysis with free sialic acid derivatives revealed that the MHV-S HE protein specifically de-O-acetylates 5-N-acetyl-4-O-acetyl sialic acid (Neu4, 5Ac2) but not 5-N-acetyl-9-O-acetyl sialic acid (Neu5,9Ac2), which is the major substrate for esterases of influenza C virus and bovine coronaviruses. In addition, the MHV-S esterase converted glycosidically bound Neu4,5Ac2 of guinea pig serum glycoproteins to Neu5Ac. By expression of the MHV esterase with recombinant vaccinia virus and incubation with guinea pig serum, we demonstrated that the viral HE possesses sialate-4-O-acetylesterase activity. In addition to observed enzymatic activity, MHV-S exhibited affinity to guinea pig and horse serum glycoproteins. Binding required sialate-4-O-acetyl groups and was abolished by chemical de-O-acetylation. Since Neu4,5Ac2 has not been identified in mice, the nature of potential substrates and/or secondary receptors for MHV-S in the natural host remains to be determined. The esterase of MHV-S is the first example of a viral enzyme with high specificity and affinity toward 4-O-acetylated sialic acids.  (+info)

Lysosomal and cytosolic sialic acid 9-O-acetylesterase activities can Be encoded by one gene via differential usage of a signal peptide-encoding exon at the N terminus. (3/152)

9-O-Acetylation is one of the most common modifications of sialic acids, and it can affect several sialic acid-mediated recognition phenomena. We previously reported a cDNA encoding a lysosomal sialic acid-specific 9-O-acetylesterase, which traverses the endoplasmic reticulum-Golgi pathway and localizes primarily to lysosomes and endosomes. In this study, we report a variant cDNA derived from the same gene that contains a different 5' region. This cDNA has a putative open reading frame lacking a signal peptide-encoding sequence and is thus a candidate for the previously described cytosolic sialic acid 9-O-acetylesterase activity. Epitope-tagged constructs confirm that the new sequence causes the protein product to be targeted to the cytosol and has esterase activity. Using reverse transcription-polymerase chain reaction to distinguish the two forms of message, we show that although the lysosomal sialic acid-specific 9-O-acetylesterase message has a widespread pattern of expression in adult mouse tissues, this cytosolic sialic acid 9-O-acetylesterase form has a rather restricted distribution, with the strongest expression in the liver, ovary, and brain. Using a polyclonal antibody directed against the 69-amino acid region common to both proteins, we confirmed that the expression of glycosylated and nonglycosylated polypeptides occurred in appropriate subcellular fractions of normal mouse tissues. Rodent liver polypeptides reacting to the antibody also co-purify with previously described lysosomal sialic acid esterase activity and at least a portion of the cytosolic activity. Thus, two sialic acid 9-O-acetylesterases found in very different subcellular compartments can be encoded by a single gene by differential usage of a signal peptide-encoding exon at the N terminus. The 5'-rapid amplification of cDNA ends results and the differences in tissue-specific expression suggest that expression of these two products may be differentially regulated by independent promoters.  (+info)

Characterization of an acetyl xylan esterase from the anaerobic fungus Orpinomyces sp. strain PC-2. (4/152)

A 1,067-bp cDNA, designated axeA, coding for an acetyl xylan esterase (AxeA) was cloned from the anaerobic rumen fungus Orpinomyces sp. strain PC-2. The gene had an open reading frame of 939 bp encoding a polypeptide of 313 amino acid residues with a calculated mass of 34,845 Da. An active esterase using the original start codon of the cDNA was synthesized in Escherichia coli. Two active forms of the esterase were purified from recombinant E. coli cultures. The size difference of 8 amino acids was a result of cleavages at two different sites within the signal peptide. The enzyme released acetate from several acetylated substrates, including acetylated xylan. The activity toward acetylated xylan was tripled in the presence of recombinant xylanase A from the same fungus. Using p-nitrophenyl acetate as a substrate, the enzyme had a K(m) of 0.9 mM and a V(max) of 785 micromol min(-1) mg(-1). It had temperature and pH optima of 30 degrees C and 9.0, respectively. AxeA had 56% amino acid identity with BnaA, an acetyl xylan esterase of Neocallimastix patriciarum, but the Orpinomyces AxeA was devoid of a noncatalytic repeated peptide domain (NCRPD) found at the carboxy terminus of the Neocallimastix BnaA. The NCRPD found in many glycosyl hydrolases and esterases of anaerobic fungi has been postulated to function as a docking domain for cellulase-hemicellulase complexes, similar to the dockerin of the cellulosome of Clostridium thermocellum. The difference in domain structures indicated that the two highly similar esterases of Orpinomyces and Neocallimastix may be differently located, the former being a free enzyme and the latter being a component of a cellulase-hemicellulase complex. Sequence data indicate that AxeA and BnaA might represent a new family of hydrolases.  (+info)

Acetylation: a regulatory modification to rival phosphorylation? (5/152)

The fact that histones are modified by acetylation has been known for almost 30 years. The recent identification of enzymes that regulate histone acetylation has revealed a broader use of this modification than was suspected previously. Acetylases are now known to modify a variety of proteins, including transcription factors, nuclear import factors and alpha-tubulin. Acetylation regulates many diverse functions, including DNA recognition, protein-protein interaction and protein stability. There is even a conserved structure, the bromodomain, that recognizes acetylated residues and may serve as a signalling domain. If you think all this sounds familiar, it should be. These are features characteristic of kinases. So, is acetylation a modification analogous to phosphorylation? This review sets out what we know about the broader substrate specificity and regulation of acetyl- ases and goes on to compare acetylation with the process of phosphorylation.  (+info)

Rhamnogalacturonan acetylesterase elucidates the structure and function of a new family of hydrolases. (6/152)

BACKGROUND: The complex polysaccharide rhamnogalacturonan constitutes a major part of the hairy region of pectin. It can have different types of carbohydrate sidechains attached to the rhamnose residues in the backbone of alternating rhamnose and galacturonic acid residues; the galacturonic acid residues can be methylated or acetylated. Aspergillus aculeatus produces enzymes that are able to perform a synergistic degradation of rhamnogalacturonan. The deacetylation of the backbone by rhamnogalacturonan acetylesterase (RGAE) is an essential prerequisite for the subsequent action of the enzymes that cleave the glycosidic bonds. RESULTS: The structure of RGAE has been determined at 1.55 A resolution. RGAE folds into an alpha/beta/alpha structure. The active site of RGAE is an open cleft containing a serine-histidine-aspartic acid catalytic triad. The position of the three residues relative to the central parallel beta sheet and the lack of the nucleophilic elbow motif found in structures possessing the alpha/beta hydrolase fold show that RGAE does not belong to the alpha/beta hydrolase family. CONCLUSIONS: Structural and sequence comparisons have revealed that, despite very low sequence similarities, RGAE is related to seven other proteins. They are all members of a new hydrolase family, the SGNH-hydrolase family, which includes the carbohydrate esterase family 12 as a distinct subfamily. The SGNH-hydrolase family is characterised by having four conserved blocks of residues, each with one completely conserved residue; serine, glycine, asparagine and histidine, respectively. Each of the four residues plays a role in the catalytic function.  (+info)

Three multidomain esterases from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17 that carry divergent dockerin sequences. (7/152)

Three enzymes carrying esterase domains have been identified in the rumen cellulolytic anaerobe Ruminococcus flavefaciens 17. The newly characterized CesA gene product (768 amino acids) includes an N-terminal acetylesterase domain and an unidentified C-terminal domain, while the previously characterized XynB enzyme (781 amino acids) includes an internal acetylesterase domain in addition to its N-terminal xylanase catalytic domain. A third gene, xynE, is predicted to encode a multidomain enzyme of 792 amino acids including a family 11 xylanase domain and a C-terminal esterase domain. The esterase domains from CesA and XynB share significant sequence identity (44%) and belong to carbohydrate esterase family 3; both domains are shown here to be capable of deacetylating acetylated xylans, but no evidence was found for ferulic acid esterase activity. The esterase domain of XynE, however, shares 42% amino acid identity with a family 1 phenolic acid esterase domain identified from Clostridum thermocellum XynZ. XynB, XynE and CesA all contain dockerin-like regions in addition to their catalytic domains, suggesting that these enzymes form part of a cellulosome-like multienzyme complex. The dockerin sequences of CesA and XynE differ significantly from those previously described in R. flavefaciens polysaccharidases, including XynB, suggesting that they might represent distinct dockerin specificities.  (+info)

The acetyl xylan esterase of Bacillus pumilus belongs to a family of esterases with broad substrate specificity. (8/152)

The Bacillus pumilus gene encoding acetyl xylan esterase (axe) was identified and characterized. The axe gene was expressed and the recombinant enzyme produced in Escherichia coli was purified and characterized. The recombinant enzyme displayed similar properties to the acetyl xylan esterase (AXE) purified from B. pumilus. The AXE primary structure was 76% identical to the cephalosporin C deacetylase of B. subtilis, and 40% to two recently identified AXEs from Thermoanaerobacterium and Thermotoga maritima. These four proteins are of similar size and represent a new family of esterases having a broad substrate specificity. The recombinant AXE was demonstrated to have activity on several acetylated substrates, including on cephalosporin C.  (+info)

Cell wall hemicelluloses and pectins are O-acetylated at specific positions, but the significance of these substitutions is poorly understood. Using a transgenic approach, we investigated how reducing the extent of O-acetylation in xylan affects cell wall chemistry, plant performance and the recalcitrance of lignocellulose to saccharification. The Aspergillus niger acetyl xylan esterase AnAXE1 was expressed in Arabidopsis under the control of either the constitutively expressed 35S CAMV promoter or a woody-tissue-specific GT43B aspen promoter, and the protein was targeted to the apoplast by its native signal peptide, resulting in elevated acetyl esterase activity in soluble and wall-bound protein extracts and reduced xylan acetylation. No significant alterations in cell wall composition were observed in the transgenic lines, but their xylans were more easily digested by a beta-1,4-endoxylanase, and more readily extracted by hot water, acids or alkali. Enzymatic saccharification of lignocellulose ...
TY - JOUR. T1 - Regioselective deacetylation of cellulose acetates by acetyl xylan esterases of different CE-families. AU - Altaner, Clemens. AU - Saake, Bodo. AU - Tenkanen, Maija. AU - Eyzaguirre, Jaime. AU - Faulds, Craig B.. AU - Biely, Peter. AU - Viikari, Liisa. AU - Siika-aho, Matti. AU - Puls, Jurgen. PY - 2003. Y1 - 2003. N2 - Cellulose acetate (CA) was found to be a substrate of several acetyl xylan esterases (AXE). Eight AXE from different carbohydrate esterase (CE) families were tested on their activity against CA with a degree of substitution of 0.7 and 1.4. The classification of the AXEs into CE families according to their structure by hydrophobic cluster analysis followed clearly their activity against CA. Within the same CE family similar, and between the CE families different deacetylation behaviours could be observed. Furthermore, each esterase family showed a distinct regioselective mode of action. The CE 1 family enzymes regioselectively cleaved the substituents in C2- and ...
Erwinia chrysanthemi causes soft-rot diseases of various plants byenzymatic degradation of the pectin in plant cell walls. Pectin is acomplex polysaccharide. The main chain is constituted of galacturonateresidues, and some of them are modified by methyl and/or acetylesterification. Esterases are necessary to remove these modifications and,thus, to facilitate the further degradation of the polysaccharidic chain.In addition to PaeY, the first pectin acetylesterase identified in the E.chrysanthemi strain 3937, we showed that this bacterium produces a secondpectin acetylesterase encoded by the gene paeX. The paeX open readingframe encodes a 322-residue precursor protein of 34,940 Da, including a21-amino-acid signal peptide. Analysis of paeX transcription, by usinggene fusions, revealed that it is induced by pectic catabolic products andaffected by catabolite repression. The expression of paeX is regulated bythe repressor KdgR, which controls all the steps of pectin catabolism; bythe repressor PecS, ...
The primary structure of AtPAEs revealed that they are unlikely to be membrane proteins and that most isoforms have a basic pI, as suggested in the early literature [17]. However, the predicted GPI anchor for AtPAE10 and AtPAE12, both from clade 2, could indicate a putative role in plant signaling and some interaction with the plasma membrane. The presence of such a predicted GPI anchor has been observed in other pectin-related genes, including PMEI [55]. The absence of a predicted signaling sequence for three AtPAEs, AtPAE2, AtPAE4 and AtPAE5, indicates that some Arabidopsis PAEs without a signal peptide could have a functional activity. Similar results have been observed with several PMEs from distinct plant species and particularly for AtPME31, which has no signaling sequence but is active and cannot be inhibited by the kiwi pectin methylesterase inhibitor, a strong PME inhibitor [56]. 3D homology modeling of AtPME31 revealed an external loop. The function and the mechanism by which AtPME31 ...
Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively regulates MalT activity by antagonizing maltotriose binding. Inhibits MelA galactosidase activity.
In molecular biology, haemagglutinin-esterase fusion glycoprotein (HEF) is a multi-functional protein embedded in the viral envelope of several viruses, including influenza C virus, coronaviruses and toroviruses. HEF is required for infectivity, and functions to recognise the host cell surface receptor, to fuse the viral and host cell membranes, and to destroy the receptor upon host cell infection. The haemagglutinin region of HEF is responsible for receptor recognition and membrane fusion, and bears a strong resemblance to the sialic acid-binding haemagglutinin found in influenza A and B viruses, except that it binds 9-O-acetylsialic acid. The esterase region of HEF is responsible for the destruction of the receptor, an action that is carried out by neuraminidase in influenza A and B viruses. The esterase domain is similar in structure to Streptomyces scabies esterase, and to acetylhydrolase, thioesterase I and rhamnogalacturonan acetylesterase. The haemagglutinin-esterase glycoprotein HEF must ...
Colorimetric detection of reaction products is typically preferred for initial surveys of acetyl xylan esterase (AcXE) activity. This chapter will describe common colorimetric methods, and variations...
Late enzymes of vindoline biosynthesis. S-Adenosyl-L-methionine:11-O-demethyl-17-O-deacetylvindoline 11-O-methylase and unspecific acetylesterase ...
Hydrolases Choi JH, Jeong KJ, Kim SC, Lee SY; Appl Microbiol Biotechnol. 2000 Jun; 53(6): 640-5. Efficient secretory production of alkaline phosphatase by high cell density culture of recombinant Escherichia coli using the Bacillus sp. endoxylanase signal sequence.PubMed. Farias T, Mandrich L, Rossi M, Manco G; Protein Pept Lett. 2007; 14(2):165-9. Biochemical and thermostability features of acetyl esterase Aes from Escherichia coli. PubMed Jefferson RA; US 5432081 A. 1995 Jul. Host cells transformed with the E. coli glucoronide permease gene Jokerst J.C., Adkins J.A., Bisha B., Mentele M.M., Goodridge L. D., Henry C. S.; Chem.; 2012, 84:2900− ...
Chitin deacetylases (CDA) mostly occur in marine bacteria, few in insects, and several in fungi [1].. In fungi, for example, CDAs are involved in cell wall formation, sporulation, and catabolism of chitin oligosaccharides. Many plant fungal pathogens secrete CDAs during plant infection. Plants only detect fungal infections by registering chitin. Fungi "turn invisible" by deacetylating chitin into chitosan and thus, outwit the plant defence system [2].. The CDAs generate chitosan oligomers from chitin by deacetylating the N-acetylglucosamine units of the substrate [3]. During deacetylation, acetic acid is cleaved off from a glucosamine unit. Some CDAs may even deacetylate chitosan, creating a double deacetylated oligomer [2].. Chitin deacetylases belong to the carbohydrate esterase family 4. All family members, including NodB protein and chitin deacetylases, share the same primary structure called "NodB homology domain" or "polysaccharide deacetylase domain" [4].. In medical applications and ...
Enzyme saccharification of pretreated brewers spent grains (BSG) was investigated, aiming at maximising glucose production. Factors investigated were; variation of the solids loadings at different cellulolytic enzyme doses, reaction time, higher energy mixing methods, supplementation of the cellulolytic enzymes with additional enzymes (and cofactors) and use of fed-batch methods. Improved slurry agitation through aerated high-torque mixing offered small but significant enhancements in glucose yields (to 53 ± 2.9 g/L and 45% of theoretical yield) compared to only 41 ± 4.0 g/L and 39% of theoretical yield for standard shaking methods (at 15% w/v solids loading). Supplementation of the cellulolytic enzymes with additional enzymes (acetyl xylan esterases, ferulic acid esterases and α-L- arabinofuranosidases) also boosted achieved glucose yields to 58 - 69 ± 0.8 - 6.2 g/L which equated to 52 - 58% of theoretical yield. Fed-batch methods also enhanced glucose yields (to 58 ± 2.2 g/L and 35% of ...
acetyl xylan esterase (EC 3.1.1.72); chitin deacetylase (EC 3.5.1.41); chitooligosaccharide deacetylase (EC 3.5.1.-); peptidoglycan GlcNAc deacetylase (EC 3.5.1.-); peptidoglycan N-acetylmuramic acid deacetylase (EC 3.5.1.- ...
LSE CareerHub should be your main job hunting resource as an LSE student or recent graduate, but there are many other employment websites you can use in addition to maximise your chances of finding work. It is important to choose an appropriate website before starting your online job search. Typing a job title, employer and location into a general search engine like Google or Bing may lead to an occasional lucky discovery, but for a more efficient search you will require a job listings website. These websites come in two main varieties, job boards and aggregate job sites. ...
3VUS: The structure of the deacetylase domain of Escherichia coli PgaB, an enzyme required for biofilm formation: a circularly permuted member of the carbohydrate esterase 4 family
I just received my offer pack from LSE and it said nothing about a deposit I might need to pay in order to accept the offer. Still, I am not very sure what would happen if I were to accept it now and not actually register (As my situation is pretty much the same as MPAs - Im waiting for schorlarship answers). On the other hand, it does say that they are expecting an answer within 6 weeks BUT your offer will not be withdrawn if they dont get it in this time frame and you should reply as soon as possible ...
seeing so many offers coming in already has made me worried that I will be applying too late, even though I was ascertained by one staff member that i have 99% chance of getting in.......i should start my LSE app sooner now, not till i m done with my cambs whose deadline is 30th jan ...
Latest Novartis AG (0QM7:LSE) share price with interactive charts, historical prices, comparative analysis, forecasts, business profile and more.
6-sulfo sialyl Lewis X is the common receptor determinant recognized by H5, H6, H7 and H9 influenza viruses of terrestrial poultry. (2008 ...
Alfa Aesar™ N-Acetyl-3,5-dinitro-L-tyrosine, 98% 250mg Alfa Aesar™ N-Acetyl-3,5-dinitro-L-tyrosine, 98% Amino Acids
Wittenberg, Raphael and King, Derek (2020) The future incidence, prevalence and costs of stroke in the UK. Age and Ageing, 49 (2). pp. 277-282. ISSN 0002-0729 Wouters, Olivier J., McKee, Martin and Luyten, Jeroen (2020) Estimated research and development investment needed to bring a new medicine to market, 2009-2018. JAMA, 323 (9). pp. 844-853. ISSN 0098-7484 ...
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RNS Number: 0905 Z Dunelm Group plc 16 September 2020. Dunelm has been notified that on 15 September 2020, Andy Harrison, Chairman, made the following...
chains in the Genus database with same CATH superfamily 3UU2 A; 4LSI A; 2PRN A; 4AUI A; 2ZFG A; 3O0E A; 8PRN A; 1HXT A; 2XG6 A; 3T24 A; 1HXX A; 3SY9 A; 1E54 A; 4C69 X; 2FGR A; 5DL5 A; 4GCS A; 3UPG A; 1GFN A; 3SY7 A; 4BUM X; 3SYB A; 1PRN A; 2XE2 A; 3POX A; 4FT6 A; 2Y2X A; 1OSM A; 1BT9 A; 2J4U P; 4FSP A; 4KRA A; 5JDP A; 3POQ A; 1BH3 A; 3A2S X; 4FRT A; 2OMF A; 3T20 A; 4LSF A; 3VZW X; 2O4V A; 4D5U A; 4KR4 A; 4FOZ A; 3WI4 A; 3T0S A; 3VZT X; 3SZD A; 3K19 A; 5DL8 A; 2XE5 A; 2QTK A; 4LSE A; 3VZU X; 1MPF A; 3JTY A; 5DL6 A; 1PHO A; 1OPF A; 4RJX A; 4FSO A; 4RJW A; 3PRN A; 3EMN X; 7PRN A; 3POR A; 5FVN A; 5PRN A; 6PRN A; 3SYS A; 3HW9 A; 4LSH A; 1GFM A; 2Y0L A; 5DL7 A; 2XE1 A; 3HWB A; 4D64 A; 2ODJ A; 2K4T A; 3POU A; 2ZLD A; 3WI5 A; 2Y0K A; 4JFB A; 1GFQ A; 2POR A; 1HXU A; 3SZV A; 4KR8 A; 2J1N A; 3VY9 X; 1GFO A; 4FRX A; 4FMS A; 2XE3 A; 2FGQ X; 1H6S 1; 4GCQ A; 4D65 A; 1GFP A; 2Y0H A; 3VY8 X; 4GCP A; 3FYX A; 3NSG A; #chains in the Genus database with same CATH topology 2R88 A; 3UU2 A; 3AAE B; 4RL9 A; 3VE2 A; 4F4B ...
Autoimmune Addisons disease (AAD) is a debilitating condition and affected patients rely on lifelong steroid replacement. Despite treatment, many patients have increased morbidity and mortality. The diseases rarity has precluded large scale genomic or cellular studies in humans, resulting in an incomplete picture of the pathophysiology of AAD. This thesis details my research on the pathophysiology and novel therapeutic approaches in AAD. I performed two candidate gene studies on susceptibility alleles that have been implicated in other autoimmune diseases to explore potential causal pathways of these genetic determinants in AAD. The common variant 307*Ser allele of CD226 gene was found to contribute to AAD susceptibility as part of autoimmune polyendocrinopathy type 2. Two genetic variants from a panel of rare and functionally defective variants in the sialic acid acetylesterase (SIAE) gene were identified but they were not significantly associated with AAD. I explored new therapeutic ...
A glucuronoyl esterase (GE) from the thermophilic fungus Sporotrichum thermophile, belonging to the carbohydrate esterase family 15 (CE-15), was functionally expressed in the methylotrophic yeast Pich
The acetyl xylan esterase II gene from Penicillium purpurogenum is differentially expressed in several carbon sources, and tightly regulated by pH ...
casSAR Dugability of P17003 | HE | Hemagglutinin-esterase-fusion glycoprotein - Also known as HEMA_INCHY, HE. Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HEF2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Displays a receptor-destroying activity which is a neuraminidate-O-acetyl esterase. This activity cleaves off any receptor on the cell surface, which would otherwise prevent virions release. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell (By
The family 15 carbohydrate esterase (CE15) MZ0003, which derives from a marine Arctic metagenome, has a broader substrate scope than other members of this family. Here we report the crystal structure of MZ0003, which reveals that residues comprising the catalytic triad differ from previously-characterized fungal homologs, and resolves three large loop regions that are unique to this bacterial sub-clade. The catalytic triad of the bacterial CE15, which includes Asp 332 as its third member, closely resembles that of family 1 carbohydrate esterases (CE1), despite the overall lower structural similarity with members of this family ...
All cells are decorated on their surfaces with a complex array of oligosaccharides (glycans) that are attached to membrane glycoproteins and glycolipids or which make up the different components of soluble extracellular materials such as mucus and which are involved in the initial interactions of viruses or other pathogens with cells or mucosal surfaces (1). The chemical diversity of these glycans contributes to their complex functional roles in controlling cell-cell, cell-pathogen, and cell-environment interactions. Linear and branched glycoconjugates of vertebrate cells are often terminated in sialic acids (Sias). Sias have critical roles in maintaining a variety of cell functions due to their abundance as exposed terminal sugars, where they are involved in highly specific and regulated cellular lectin interactions (2, 3). They have also been exploited by many different pathogens as well as by nonpathogenic microbes for host recognition and attachment, including playing a role as receptors for ...
1JT2: Structural basis for the substrate specificity of the feruloyl esterase domain of the cellulosomal xylanase Z from Clostridium thermocellum.
Mouse monoclonal antibody raised against a full-length recombinant SIAE. SIAE (NP_733746.1, 1 a.a. ~ 523 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. (H00054414-M) - Products - Abnova
SWISS-MODEL Template Library (SMTL) entry for 1lse.1. THE INFLUENCE OF TEMPERATURE ON LYSOZYME CRYSTALS. STRUCTURE AND DYNAMICS OF PROTEIN AND WATER
The best way to accommodate the young, the Scots and the Irish is to continue to allow free movement. But the only way to contain immigration is to introduce some new controls.. Assessing the balance between free movement and control of immigration should take account not only of the costs of access to the single market, such as the net UK fiscal contribution and the acknowledged problems of the EU, but also its benefits. Membership of the worlds largest market brings direct economic benefits, greater influence over trade rules and greater leverage in negotiations with third parties. The single market is so large that no other trading power can ignore its clout in negotiations. And as discussed, Brits also benefit from immigration, and many benefit from living in other EU countries.. A partial solution is a proposal by my LSE colleague Richard Bronk to exclude students and young graduates from net-migration numbers and exempt students and recent graduates of bona fide university courses below, ...
Detailed Share Price, Charts and News for Diurnal Group Plc [LSE,DNL]. Share Market Tools for Successful Investing. Global Share Market database including US, Canada, London, Singapore, Australia, Hong Kong, and New Zealand.
In the largest international listing by any Indian firm, the Ruias-managed Essar Energy Ltd on Thursday announced plans to raise $2.5 billion (Rs 11,250 crore) through an initial public offering on the London Stock Exchange (LSE) to institutional investors.
Opens the Highlight Feature Bar and highlights feature annotations from the FEATURES table of the record. The Highlight Feature Bar can be used to navigate to and highlight other features and provides links to display the highlighted region separately. Links in the FEATURES table will also highlight the corresponding region of the sequence. More... ...
Scholars estimating policy positions from political texts typically code words or sentences and then build left-right policy scales based on the relative frequencies of text units coded into different categories. Here we reexamine such scales and propose a theoretically and linguistically superior alternative based on the logarithm of odds-ratios. We contrast this scale with the current approach of the Comparative Manifesto Project (CMP), showing that our proposed logit scale avoids widely acknowledged flaws in previous approaches. We validate the new scale using independent expert surveys. Using existing CMP data, we show how to estimate more distinct policy dimensions, for more years, than has been possible before, and make this dataset publicly available. Finally, we draw some conclusions about the future design of coding schemes for political texts.. ...
Inge Graef looks at the implications of the European Commissions leaked Digital Single Market strategy document for over-the-top players.
The UPD (Urbanisation, Planning and Development) research cluster (formerly Cities Research Cluster) brings together a key group of staff in the
RNS Number: 6053 R Banco Santander S.A. 30 June 2020. Banco Santander, S.A., in compliance with the Securities Market legislation, hereby announces:....
Shire plc (LSE: SHP, NASDAQ: SHPGY) the global specialty biopharmaceutical company, announces results for the year to December 31, 2009.
Substances: 4-Formylaminoantipyrin (4-FAA) (WSJBSKRPKADYRQ-UHFFFAOYSA-N) 5-Fluoro Cytosine (XRECTZIEBJDKEO-UHFFFAOYSA-N) 2-4-dimethylphenylformamide (JOFDPSBOUCXJCC-UHFFFAOYSA-N) 4-Acetamidoantipyrin ( N-Acetyl-4-Aminoantipyrin 4-AAA) (OIAGWXKSCXPNNZ-UHFFFAOYSA-N) Acetochlor-ESA (HXAIQOCRALNGKB-UHFFFAOYSA-N) Filter Results ...
This page lists all the Auxiliary Activity (AA) Family pages in CAZypedia that have been given [[:Category:Curator approved,Curator Approved]] status, as well as those that are currently [[:Category:Under construction,under construction]]. ==Overview== The original CAZy classification scheme aimed to describe the families of enzymes that cleave or build complex carbohydrates and the associated carbohydrate binding modules. The discovery that members of families [[CBM33]] and family [[GH61]] are lytic polysaccharide monooxygenases (LPMO), gave impetus to a reclassification of these families into a new category, the "Auxiliary Activities" ,cite>Levasseur2013,/cite>. The auxiliary activities are families of catalytic proteins that are potentially involved in plant cell degradation through an ability to help the original [[Glycoside_Hydrolase_Families,GH]], [[Polysaccharide Lyase Families,PL]] and [[Carbohydrate Esterase Families,CE]] enzymes gain access to the carbohydrates comprising the ...
Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HEF2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Displays a receptor-destroying activity which is a neuraminidate-O-acetyl esterase. This activity cleaves off any receptor on the cell surface, which would otherwise prevent virions release. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell (By similarity).
1( the rid book a primer in social choice theory revised edition lse perspectives in controls) and the center school. book a primer in social choice theory revised edition lse perspectives school and buy these clerks to hold content. generate the book a primer in social of each software with a control corner. go these blocks in Column K. Record these cells in Column L. Record this book a primer in social choice theory revised edition lse perspectives in on labour 5. thank this book on content 6. like this book a in Column N. continuing integrin - Woody Shrub? coordinate in the book a primer in social choice theory run at the pocket of Sheet L2. Photo Plot Data for Sheet L2. 5) if the book a primer in social choice theory revised edition lse perspectives in can Let signed. have this weeks in Columns C and E. Describe the old book of each web century. Appendix 6 for the Supervised data. Classified book a primer in social choice theory activity role the ARC leukocyte to the reference. win this book ...
Using sialic acid analogues to define the molecular basis of complement resistance mediated by sialylation of neisseria gonorrhoeae lipopolysaccharide and to design novel therapeutics
Expression of SIAE (CSE-C, LSE, MGC87009, YSG2) in oral mucosa tissue. Antibody staining with HPA038052 and HPA038053 in immunohistochemistry.
If that were all there was today it would not be bad. However, we will also do more on the riveting battle for ITV. BSkyBs Friday purchase of 17.9 per cent of ITV will be discussed at Ofcom today but whether it goes further depends on their definition of "material influence" and whether BSkyB now has it at ITV. It is worth noting that Nasdaq has been allowed to hold 25 per cent of the LSE. But the media tends to get treated differently. Will Whitehorn of Virgin was on Radio Five Live last night with Jeff Randall and me, making an impassioned but not entirely convincing case. Channel 4 chairman Luke Johnson was on as well. He saw Skys move as a simple attempt to thwart NTL but took the opportunity also to say how amazing it is that ITVs board will probably have taken at least six months to find a chief executive, when it took him six weeks at Channel 4. BSkyB shares are down only 1.3 per cent this morning and ITV shares, having fallen sharply at first this morning, are now down just 1.4 per ...
Speaker(s): Margaret Busby, Sarah Ladipo Manyika | African literature is increasingly esteemed around the world, but the true extent of its global historic influence remains largely overlooked. Negoti... - タブレット、携帯電話、またはブラウザで Africa Talks: the global legacy of African women writers [Audio]×LSE: Public lectures and events を今すぐ聴こう。ダウンロードは不要です。
The LSE Companion to Health Policy covers a wide range of conceptual and practical issues from a number of different perspectives introducing the reader to, and summarising, the vast literature that analyses the complexities of health policy. The Companion also assesses the current state of the art ...
"Investigation of β-xylosidase, α-L-arabinofuranosidase and acetylesterase from Thermotoga hypogea." (2008). Dworkin, Martin, ...
... thioesterase I and rhamnogalacturonan acetylesterase. The haemagglutinin-esterase glycoprotein HEF must be cleaved by the ...
Polz, L.; Schübel, H.; Stöckigt, J. (1987). "Characterization of 2β(R)-17-O-acetylajmalan:acetylesterase-a specific enzyme ... Acetylajmaline esterase (EC 3.1.1.80, AAE, 2beta(R)-17-O-acetylajmalan:acetylesterase, acetylajmalan esterase) is an enzyme ...
11-O-demethyl-17-O-deacetylvindoline 11-O-methylase and unspecific acetylesterase". Plant Cell Rep. 4: 337-340. doi:10.1007/ ...
... cephalosporin C acetylesterase, cephalosporin C acetyl-esterase, and cephalosporin C deacetylase. This enzyme participates in ... Other names in common use include cephalosporin C acetyl-hydrolase, cephalosporin C acetylase, cephalosporin acetylesterase, ...
The second reaction is exactly the same as chemical hydrolysis with the exception of the use of an acetylesterase enzyme. The ... Deacetylation can be performed by either chemical hydrolysis or acetylesterase. Chemical hydrolysis is the cleavage of a ... The major factor that affects the biodegradation duration is the availability of acetylesterase and cellulase enzymes. Without ... "Acetylesterase-mediated deacetylation of pectin impairs cell elongation, pollen germination, and plant reproduction". Plant ...
Acetylesterase (EC 3.1.1.6), splits off acetyl groups Cholinesterase Acetylcholinesterase, inactivates the neurotransmitter ...
... acetylesterase MeSH D08.811.277.352.100.100 --- carboxylesterase MeSH D08.811.277.352.100.150 --- cholesterol esterase MeSH ...
... acyloxyacyl hydrolase and sialic acid acetylesterase Some amidases, including fatty acid amide hydrolase Some peptidases, ...
... acetylesterase EC 3.1.1.7: acetylcholinesterase EC 3.1.1.8: cholinesterase EC 3.1.1.9: deleted EC 3.1.1.10: tropinesterase EC ... rhamnogalacturonan acetylesterase EC 3.1.1.87: fumonisin B1 esterase EC 3.1.1.88: pyrethroid hydrolase EC 3.1.1.89: protein ... sialate O-acetylesterase EC 3.1.1.54: acetoxybutynylbithiophene deacetylase EC 3.1.1.55: acetylsalicylate deacetylase EC 3.1. ...
In enzymology, an acetylesterase (EC 3.1.1.6) is an enzyme that catalyzes the chemical reaction an acetic ester + H2O ⇌ {\ ... Jansen EF; Nutting M-DF; Balls AK (1948). "The reversible inhibition of acetylesterase by diisopropyl fluorophosphate and ... and Citrus acetylesterase. As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes ...
... (EC 3.1.1.86, RGAE) is an enzyme with systematic name rhamnogalacturonan 2/3-O-acetyl-alpha-D ... Rhamnogalacturonan acetylesterase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and Cellular ... Molgaard, A.; Kauppinen, S.; Larsen, S. (2000). "Rhamnogalacturonan acetylesterase elucidates the structure and function of a ... "Molecular cloning and characterization of a rhamnogalacturonan acetylesterase from Aspergillus aculeatus. Synergism between ...
... (SIAE) is a protein that in human is encoded by the SIAE gene located on chromosome 11. The SIAE ... In enzymology, a sialate O-acetylesterase (EC 3.1.1.53) is an enzyme that catalyzes the chemical reaction N-acetyl-O- ... 2004). "A Gene Encoding Sialic-Acid-Specific 9-O-Acetylesterase Found in Human Adult Testis". J. Biomed. Biotechnol. 2004 (3): ... 2014). "A missense single-nucleotide polymorphism in the sialic acid acetylesterase (SIAE) gene is associated with anti-PIT-1 ...
... (SIAE) is an enzyme that produces 9-O acetylated sialic acid e.g. in mammals. It down-regulates B ... 8 July 2010). "Functionally defective germline variants of sialic acid acetylesterase in autoimmunity". Nature. 466 (7303): 243 ...
... resulting in elevated acetyl esterase activity in soluble and wall-bound protein extracts and reduced xylan acetylation. No ...
In enzymology, an acetylesterase (EC 3.1.1.6) is an enzyme that catalyzes the chemical reaction an acetic ester + H2O ⇌ {\ ... Jansen EF; Nutting M-DF; Balls AK (1948). "The reversible inhibition of acetylesterase by diisopropyl fluorophosphate and ... and Citrus acetylesterase. As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes ...
Rhamnogalacturonan acetylesterase (EC 3.1.1.86, RGAE) is an enzyme with systematic name rhamnogalacturonan 2/3-O-acetyl-alpha-D ... Rhamnogalacturonan acetylesterase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and Cellular ... Molgaard, A.; Kauppinen, S.; Larsen, S. (2000). "Rhamnogalacturonan acetylesterase elucidates the structure and function of a ... "Molecular cloning and characterization of a rhamnogalacturonan acetylesterase from Aspergillus aculeatus. Synergism between ...
Sialate O-acetylesterase domain (IPR005181). Short name: SASA Overlapping homologous superfamilies *SGNH hydrolase superfamily ... Functionally defective germline variants of sialic acid acetylesterase in autoimmunity.. Nature 466 243-7 2010 ...
Acetyl esteraseAdd BLAST. 319. Proteomic databases. PaxDb, a database of protein abundance averages across all three domains of ... Acetyl esteraseUniRule annotation. ,p>Manual validated information which has been generated by the UniProtKB automatic ... IPR023508. Acetyl_esterase. IPR002168. Lipase_GDXG_HIS_AS. IPR033140. Lipase_GDXG_put_SER_AS. ... IPR023508. Acetyl_esterase. IPR002168. Lipase_GDXG_HIS_AS. IPR033140. Lipase_GDXG_put_SER_AS. ...
Rhamnogalacturonan acetylesterase with seven N-linked carbohydrate residues distributed at two N-glycosylation sites refined at ... Rhamnogalacturonan acetylesterase, a member of the SGNH-hydrolase family.. Molgaard, A.. () TO BE PUBLISHED --: -- ... Rhamnogalacturonan acetylesterase elucidates the structure and function of a new family of hydrolases.. Molgaard, A.,Kauppinen ... Rhamnogalacturonan acetylesterase with seven N-linked carbohydrate residues distributed at two N-glycosylation sites refined at ...
Activity of influenza C virus O-acetylesterase with O-acetyl-containing compounds A Garcia-Sastre; A Garcia-Sastre ... A Garcia-Sastre, E Villar, J C Manuguerra, C Hannoun, J A Cabezas; Activity of influenza C virus O-acetylesterase with O-acetyl ... We conclude that the O-acetylesterase from influenza C virus has a broad specificity towards both synthetic and natural non- ... purified and used as source for the enzyme O-acetylesterase (N-acyl-O-acetylneuraminate O-acetylhydrolase; EC 3.1.1.53). This ...
Characterization of a sialate O-acetyl esterase (NanS) from the oral pathogen Tannerella forsythia that enhances sialic acid ... In summary, we have characterized a novel sialate-O-acetylesterase that contributes to siallobiology of this important human ... Characterization of a sialate O-acetyl esterase (NanS) from the oral pathogen Tannerella forsythia that enhances sialic acid ... Characterization of a sialate O-acetyl esterase (NanS) from the oral pathogen Tannerella forsythia that enhances sialic acid ...
Pectin acetylesterase Arabidopsis thaliana Phylogenetic treeConserved motifs3D homology. Background. The plant primary cell ... Gou J-Y, Miller LM, Hou G, Yu X-H, Chen X-Y, Liu C-J. Acetylesterase-Mediated Deacetylation of Pectin Impairs Cell Elongation, ... A novel protein from mung bean hypocotyl cell walls with acetyl esterase activity. Phytochemistry. 1995;38:315-9.View Article ... The degree of acetylation of pectin can be modulated by pectin acetylesterase (EC 3.1.1.6, PAE). The function and structure of ...
Functional and structural characterization of a thermostable acetyl esterase from Thermotoga maritima. Levisson M., Han GW., ... No activity was detected on xylan or acetylated xylan, which implies that TM0077 is an acetyl esterase and not an acetyl xylan ... Acetylesterase, Catalytic Domain, Computer Simulation, Crystallography, X-Ray, Enzyme Stability, Hot Temperature, Hydrogen-Ion ...
The possible role of a cephalosporin C acetyl esterase in the formation of the β-lactam antibiotic A16886A, 7-(5-amino-5- ... Detection of a Cephalosporin C Acetyl Esterase in the Carbamate Cephalosporin Antibiotic-Producing Culture, Streptomyces ...
RHAMNOGALACTURONAN ACETYLESTERASE FROM ASPERGILLUS ACULEATUS AT 1.9 A RESOLUTION ... RHAMNOGALACTURONAN ACETYLESTERASE FROM ASPERGILLUS ACULEATUS AT 1.9 A RESOLUTION. Coordinates. PDB Format Method. X-RAY ... Molgaard, A. et al., Rhamnogalacturonan acetylesterase elucidates the structure and function of a new family of hydrolases. ...
A panel of rare and functionally defective genetic variants in the sialic acid acetylesterase (SIAE) gene has recently been ... The role of functionally defective rare germline variants of sialic acid acetylesterase in autoimmune Addisons disease.. Gan ...
Thus, rhamnogalacturonan acetyl esterase belonging to CE-12 family has gained significance in deacetylation of these residues ... Rhamnogalacturonan acetylesterase flavodoxin-like (SGNH hydrolase). Rhamnogalacturonan present in the cell walls. RGAE ... Mølgaard A, Kauppinen S, Larsen S (2000) Rhamnogalacturonan acetylesterase elucidates the structure and function of a new ... The three-dimensional structure of the rhamnogalacturonan acetylesterase (RGAE) from Aspergillus aculeatus was revealed by ( ...
Rhamnogalacturonan acetylesterase elucidates the structure and function of a new family of hydrolases.. Structure 8 373-83 2000 ... A branched N-linked glycan at atomic resolution in the 1.12 A structure of rhamnogalacturonan acetylesterase.. Acta Crystallogr ... fungal rhamnogalacturonan acetylesterase [PMID: 11752785]; and the multifunctional enzyme thioesterase I (TAP) from Escherichia ...
Acetylesterase / antagonists & inhibitors* * DNA-Binding Proteins / physiology* * Enzyme Inhibitors / pharmacology* * HIV Long ...
Acetylesterase. 3.1.1.6. α-N-Acetylgalactosaminidase. 3.2.1.49. β-N-Acetylglucosaminidase 3.2.1.30. ...
Aes; Acetyl esterase/lipase [Lipid transport and metabolism]. pfam00135. Location:24 → 547. COesterase; Carboxylesterase family ... Aes; Acetyl esterase/lipase [Lipid transport and metabolism]. pfam00135. Location:25 → 548. COesterase; Carboxylesterase family ... Aes; Acetyl esterase/lipase [Lipid transport and metabolism]. pfam00135. Location:24 → 546. COesterase; Carboxylesterase family ... Aes; Acetyl esterase/lipase [Lipid transport and metabolism]. pfam00135. Location:25 → 547. COesterase; Carboxylesterase family ...
Aes; Acetyl esterase/lipase [Lipid transport and metabolism]. cl21494. Location:14 → 345. Abhydrolase; alpha/beta hydrolases. ... Aes; Acetyl esterase/lipase [Lipid transport and metabolism]. pfam00135. Location:14 → 448. COesterase; Carboxylesterase family ... Aes; Acetyl esterase/lipase [Lipid transport and metabolism]. pfam00135. Location:23 → 439. COesterase; Carboxylesterase family ... Aes; Acetyl esterase/lipase [Lipid transport and metabolism]. pfam00135. Location:46 → 613. COesterase; Carboxylesterase family ...
Acetylesterase. An enzyme that catalyzes the conversion of acetate esters and water to alcohols and acetate. EC 3.1.1.6. ...
Acetylesterase. An enzyme that catalyzes the conversion of acetate esters and water to alcohols and acetate. EC 3.1.1.6. ...
What is Acetylesterase?. Acetylesterase is an enzyme that catalyzes the chemical reaction ...
PECTIN ACETYLESTERASE - ANALYSIS AND APPLICATION FOR SUGAR BEET PECTIN UTILIZATION - (Proceedings) Savary, B.J., Nunez, A., Liu ... Pectin acetylesterase - analysis and application for sugar beet pectin utilization. Proceedings of the 1st Joint International ... PECTIN ACETYLESTERASE - ANALYSIS AND APPLICATION FOR SUGAR BEET PECTIN UTILIZATION - (Abstract Only) ... Pectin acetylesterase - analysis and application for sugar beet pectin utilization. Abstract for 1st Joint International ...
sialate O-acetylesterase activity Source: UniProtKB. Complete GO annotation on QuickGO .... GO - Biological processi. *fusion ...
0 (Carbohydrates); 0 (Multienzyme Complexes); 0 (Xylans); EC 3.1.1.6 (Acetylesterase); EC 3.1.1.72 (acetylxylan esterase); EC ...
  • In this study we performed bioinformatic and biochemical characterization of a putative sialate-O-acetylesterase from T. forsythia (NanS), which contains two putative SGNH-hydrolase domains related to sialate-O-acetylesterases from a range of organisms. (biochemj.org)
  • In summary, we have characterized a novel sialate-O-acetylesterase that contributes to siallobiology of this important human pathogen and has potential applications in analysis of sialic acid diacetylation of biologics in the pharmaceutical industry. (biochemj.org)
  • Torovirus HEs form homodimers with sialate- O -acetylesterase domains almost identical to corresponding domains in orthomyxo- and coronavirus HEs, but with unique lectin sites. (pnas.org)
  • collectively referred to as "nidoviruses" throughout) and influenza C virus (family Orthomyxoviridae ) attach to O -acetylated Sias and encode hemagglutinin-esterase proteins (HEs) with sialate- O -acetylesterase RDE activity to reverse off-target attachment ( 2 - 6 ). (pnas.org)
  • The enzymes from microsomes also catalyse the reactions of EC 3.1.1.2 (arylesterase), EC 3.1.1.5 (lysophospholipase), EC 3.1.1.6 (acetylesterase), EC 3.1.1.23 (acylglycerol lipase), EC 3.1.1.28 (acylcarnitine hydrolase), EC 3.1.2.2 (palmitoyl-CoA hydrolase), EC 3.5.1.4 (amidase) and EC 3.5.1.13 (aryl-acylamidase). (qmul.ac.uk)
  • The major factor that affects the biodegradation duration is the availability of acetylesterase and cellulase enzymes. (sarahfobes.com)
  • In enzymology, an acetylesterase (EC 3.1.1.6) is an enzyme that catalyzes the chemical reaction an acetic ester + H2O ⇌ {\displaystyle \rightleftharpoons } an alcohol + acetate Thus, the two substrates of this enzyme are acetic ester and H2O, whereas its two products are alcohol and acetate. (wikipedia.org)