AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation Science
AcetylationHistonesHistone Acetyltransferasesp300-CBP Transcription FactorsAcetyltransferasesLysineHistone DeacetylasesHistone Deacetylase InhibitorsHydroxamic AcidsChromatinProtein Processing, Post-TranslationalCREB-Binding ProteinE1A-Associated p300 ProteinNucleosomesPromoter Regions, GeneticHistone Deacetylase 1Sirtuin 1Histone Deacetylase 2Epigenesis, GeneticTranscription, GeneticButyratesChromatin ImmunoprecipitationAcetyl Coenzyme AMethylationSaccharomyces cerevisiae ProteinsChromatin Assembly and DisassemblySirtuin 3Transcription FactorsSirtuinsProtein BindingNuclear ProteinsTranscriptional ActivationArylamine N-AcetyltransferaseSirtuin 2Acetic AnhydridesCell LineMolecular Sequence DataTrans-ActivatorsGene Expression RegulationN-Terminal Acetyltransferase AButyric AcidSulfamethazineAmino Acid SequenceHeLa CellsCell Cycle ProteinsRepressor ProteinsN-Terminal Acetyltransferase ESaccharomyces cerevisiaeDNA-Binding ProteinsAnacardic AcidsCell Line, TumorMutationCell NucleusTubulinProtein Structure, TertiaryTumor Suppressor Protein p53Enzyme InhibitorsValproic AcidDNA MethylationBinding SitesGene SilencingN-Terminal AcetyltransferasesAcetylesteraseAcetate-CoA LigaseAcetatesDNACells, CulturedImmunoprecipitationHEK293 CellsChromosomal Proteins, Non-HistonePhosphorylationMass SpectrometryBlotting, WesternHistone-Lysine N-Methyltransferase
Acetylation
Formation of an acetyl derivative. (Stedman, 25th ed)
Histones
Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.
Histone Acetyltransferases
Enzymes that catalyze acyl group transfer from ACETYL-CoA to HISTONES forming CoA and acetyl-histones.
p300-CBP Transcription Factors
A family of histone acetyltransferases that is structurally-related to CREB-BINDING PROTEIN and to E1A-ASSOCIATED P300 PROTEIN. They function as transcriptional coactivators by bridging between DNA-binding TRANSCRIPTION FACTORS and the basal transcription machinery. They also modify transcription factors and CHROMATIN through ACETYLATION.
Acetyltransferases
Enzymes catalyzing the transfer of an acetyl group, usually from acetyl coenzyme A, to another compound. EC 2.3.1.
Lysine
An essential amino acid. It is often added to animal feed.
Histone Deacetylases
Deacetylases that remove N-acetyl groups from amino side chains of the amino acids of HISTONES. The enzyme family can be divided into at least three structurally-defined subclasses. Class I and class II deacetylases utilize a zinc-dependent mechanism. The sirtuin histone deacetylases belong to class III and are NAD-dependent enzymes.
Histone Deacetylase Inhibitors
Compounds that inhibit HISTONE DEACETYLASES. This class of drugs may influence gene expression by increasing the level of acetylated HISTONES in specific CHROMATIN domains.
Hydroxamic Acids
A class of weak acids with the general formula R-CONHOH.
Chromatin
The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.
Protein Processing, Post-Translational
Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.
CREB-Binding Protein
A member of the p300-CBP transcription factor family that was initially identified as a binding partner for CAMP RESPONSE ELEMENT-BINDING PROTEIN. Mutations in CREB-binding protein are associated with RUBINSTEIN-TAYBI SYNDROME.
E1A-Associated p300 Protein
A member of the p300-CBP transcription factors that was originally identified as a binding partner for ADENOVIRUS E1A PROTEINS.
Nucleosomes
The repeating structural units of chromatin, each consisting of approximately 200 base pairs of DNA wound around a protein core. This core is composed of the histones H2A, H2B, H3, and H4.
Promoter Regions, Genetic
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Histone Deacetylase 1
A histone deacetylase subtype that is found along with HISTONE DEACETYLASE 2; RETINOBLASTOMA-BINDING PROTEIN 4; and RETINOBLASTOMA-BINDING PROTEIN 7 as core components of histone deacetylase complexes.
Sirtuin 1
A sirtuin family member found primarily in the CELL NUCLEUS. It is an NAD-dependent deacetylase with specificity towards HISTONES and a variety of proteins involved in gene regulation.
Histone Deacetylase 2
A histone deacetylase subtype that is found along with HISTONE DEACETYLASE 1; RETINOBLASTOMA-BINDING PROTEIN 4; and RETINOBLASTOMA-BINDING PROTEIN 7 as core components of histone deacetylase complexes.
Epigenesis, Genetic
A genetic process by which the adult organism is realized via mechanisms that lead to the restriction in the possible fates of cells, eventually leading to their differentiated state. Mechanisms involved cause heritable changes to cells without changes to DNA sequence such as DNA METHYLATION; HISTONE modification; DNA REPLICATION TIMING; NUCLEOSOME positioning; and heterochromatization which result in selective gene expression or repression.
Transcription, Genetic
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Butyrates
Derivatives of BUTYRIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxypropane structure.
Chromatin Immunoprecipitation
A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.
Acetyl Coenzyme A
Acetyl CoA participates in the biosynthesis of fatty acids and sterols, in the oxidation of fatty acids and in the metabolism of many amino acids. It also acts as a biological acetylating agent.
Methylation
Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed)
Saccharomyces cerevisiae Proteins
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
Chromatin Assembly and Disassembly
The mechanisms effecting establishment, maintenance, and modification of that specific physical conformation of CHROMATIN determining the transcriptional accessibility or inaccessibility of the DNA.
Sirtuin 3
A sirtuin family member found primarily in MITOCHONDRIA. It is a multifunctional enzyme that contains a NAD-dependent deacetylase activity that is specific for HISTONES and a mono-ADP-ribosyltransferase activity.
Transcription Factors
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Sirtuins
A homologous family of regulatory enzymes that are structurally related to the protein silent mating type information regulator 2 (Sir2) found in Saccharomyces cerevisiae. Sirtuins contain a central catalytic core region which binds NAD. Several of the sirtuins utilize NAD to deacetylate proteins such as HISTONES and are categorized as GROUP III HISTONE DEACETYLASES. Several other sirtuin members utilize NAD to transfer ADP-RIBOSE to proteins and are categorized as MONO ADP-RIBOSE TRANSFERASES, while a third group of sirtuins appears to have both deacetylase and ADP ribose transferase activities.
Protein Binding
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Nuclear Proteins
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
Transcriptional Activation
Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.
Arylamine N-Acetyltransferase
An enzyme that catalyzes the transfer of acetyl groups from ACETYL-COA to arylamines. It can also catalyze acetyl transfer between arylamines without COENZYME A and has a wide specificity for aromatic amines, including SEROTONIN. However, arylamine N-acetyltransferase should not be confused with the enzyme ARYLALKYLAMINE N-ACETYLTRANSFERASE which is also referred to as SEROTONIN ACETYLTRANSFERASE.
Sirtuin 2
A sirtuin family member found primarily in the CYTOPLASM. It is a multifunctional enzyme that contains a NAD-dependent deacetylase activity that is specific for HISTONES and a mono-ADP-ribosyltransferase activity.
Acetic Anhydrides
Compounds used extensively as acetylation, oxidation and dehydrating agents and in the modification of proteins and enzymes.
Cell Line
Established cell cultures that have the potential to propagate indefinitely.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Trans-Activators
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
Gene Expression Regulation
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
N-Terminal Acetyltransferase A
An N-terminal acetyltransferase subtype that consists of the Naa10p catalytic subunit and the Naa15p auxiliary subunit. The structure of this enzyme is conserved between lower and higher eukaryotes. It has specificity for N-terminal SERINE; ALANINE; THREONINE; GLYCINE; VALINE; and CYSTINE residues and acts on nascent peptide chains after the removal of the initiator METHIONINE by METHIONYL AMINOPEPTIDASES.
Butyric Acid
A four carbon acid, CH3CH2CH2COOH, with an unpleasant odor that occurs in butter and animal fat as the glycerol ester.
Sulfamethazine
A sulfanilamide anti-infective agent. It has a spectrum of antimicrobial action similar to other sulfonamides.
Amino Acid Sequence
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
HeLa Cells
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Cell Cycle Proteins
Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.
Repressor Proteins
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
N-Terminal Acetyltransferase E
An N-terminal acetyltransferase subtype that consists of the Naa50p catalytic subunit, and the Naa10p and Naa15p auxiliary subunits. It has specificity for the N-terminal METHIONINE of peptides where the next amino acid in the chain is hydrophobic.
Saccharomyces cerevisiae
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
DNA-Binding Proteins
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Anacardic Acids
A group of 6-alkyl SALICYLIC ACIDS that are found in ANACARDIUM and known for causing CONTACT DERMATITIS.
Cell Line, Tumor
A cell line derived from cultured tumor cells.
Mutation
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Cell Nucleus
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Tubulin
A microtubule subunit protein found in large quantities in mammalian brain. It has also been isolated from SPERM FLAGELLUM; CILIA; and other sources. Structurally, the protein is a dimer with a molecular weight of approximately 120,000 and a sedimentation coefficient of 5.8S. It binds to COLCHICINE; VINCRISTINE; and VINBLASTINE.
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Tumor Suppressor Protein p53
Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.
Enzyme Inhibitors
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
Valproic Acid
A fatty acid with anticonvulsant properties used in the treatment of epilepsy. The mechanisms of its therapeutic actions are not well understood. It may act by increasing GAMMA-AMINOBUTYRIC ACID levels in the brain or by altering the properties of voltage dependent sodium channels.
DNA Methylation
Addition of methyl groups to DNA. DNA methyltransferases (DNA methylases) perform this reaction using S-ADENOSYLMETHIONINE as the methyl group donor.
Binding Sites
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Gene Silencing
Interruption or suppression of the expression of a gene at transcriptional or translational levels.
N-Terminal Acetyltransferases
Enzymes that catalyze the transfer of an acetyl group, usually from ACETYL COENZYME A, to the N-terminus of a peptide chain.
Acetylesterase
An enzyme that catalyzes the conversion of acetate esters and water to alcohols and acetate. EC 3.1.1.6.
Acetate-CoA Ligase
An enzyme that catalyzes the formation of CoA derivatives from ATP, acetate, and CoA to form AMP, pyrophosphate, and acetyl CoA. It acts also on propionates and acrylates. EC 6.2.1.1.
Acetates
Derivatives of ACETIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxymethane structure.
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Cells, Cultured
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Immunoprecipitation
The aggregation of soluble ANTIGENS with ANTIBODIES, alone or with antibody binding factors such as ANTI-ANTIBODIES or STAPHYLOCOCCAL PROTEIN A, into complexes large enough to fall out of solution.
HEK293 Cells
A cell line generated from human embryonic kidney cells that were transformed with human adenovirus type 5.
Chromosomal Proteins, Non-Histone
Nucleoproteins, which in contrast to HISTONES, are acid insoluble. They are involved in chromosomal functions; e.g. they bind selectively to DNA, stimulate transcription resulting in tissue-specific RNA synthesis and undergo specific changes in response to various hormones or phytomitogens.
Phosphorylation
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Mass Spectrometry
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
Blotting, Western
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Histone-Lysine N-Methyltransferase
An enzyme that catalyzes the methylation of the epsilon-amino group of lysine residues in proteins to yield epsilon mono-, di-, and trimethyllysine. EC 2.1.1.43.

UK-18892, a new aminoglycoside: an in vitro study. (1/6505)

UK-18892 is a new aminoglycoside antibiotic, a derivative of kanamycin A structurally related to amikacin. It was found to be active against a wide range of pathogenic bacteria, including many gentamicin-resistant strains. The spectrum and degree of activity of UK-18892 were similar to those of amikacin, and differences were relatively minor. UK-18892 was about twice as active as amikacin against gentamicin-susceptible strains of Pseudomonas aeruginosa. Both amikacin and UK-18892 were equally active against gentamicin-resistant strains of P. aeruginosa. There were no appreciable differences in the activity of UK-18892 and amikacin against Enterobacteriaceae and Staphylococcus aureus. Cross-resistance between these two antimicrobials was also apparent.  (+info)

Prodigious substrate specificity of AAC(6')-APH(2"), an aminoglycoside antibiotic resistance determinant in enterococci and staphylococci. (2/6505)

BACKGROUND: High-level gentamicin resistance in enterococci and staphylococci is conferred by AAC(6')-APH(2"), an enzyme with 6'-N-acetyltransferase and 2"-O-phosphotransferase activities. The presence of this enzyme in pathogenic gram-positive bacteria prevents the successful use of gentamicin C and most other aminoglycosides as therapeutic agents. RESULTS: In an effort to understand the mechanism of aminoglycoside modification, we expressed AAC(6')-APH(2") in Bacillus subtilis. The purified enzyme is monomeric with a molecular mass of 57 kDa and displays both the expected aminoglycoside N-acetyltransferase and O-phosphotransferase activities. Structure-function analysis with various aminoglycosides substrates reveals an enzyme with broad specificity in both enzymatic activities, accounting for AAC(6')-APH(2")'s dramatic negative impact on clinical aminoglycoside therapy. Both lividomycin A and paromomycin, aminoglycosides lacking a 6'-amino group, were acetylated by AAC(6')-APH(2"). The infrared spectrum of the product of paromomycin acetylation yielded a signal consistent with O-acetylation. Mass spectral and nuclear magnetic resonance analysis of the products of neomycin phosphorylation indicated that phosphoryl transfer occurred primarily at the 3'-OH of the 6-aminohexose ring A, and that some diphosphorylated material was also present with phosphates at the 3'-OH and the 3"'-OH of ring D, both unprecedented observations for this enzyme. Furthermore, the phosphorylation site of lividomycin A was determined to be the 5"-OH of the pentose ring C. CONCLUSIONS: The bifunctional AAC(6')-APH(2") has the capacity to inactivate virtually all clinically important aminoglycosides through N- and O-acetylation and phosphorylation of hydroxyl groups. The extremely broad substrate specificity of this enzyme will impact on future development of aminoglycosides and presents a significant challenge for antibiotic design.  (+info)

Probing interactions between HIV-1 reverse transcriptase and its DNA substrate with backbone-modified nucleotides. (3/6505)

BACKGROUND: To gain a molecular understanding of a biochemical process, the crystal structure of enzymes that catalyze the reactions involved is extremely helpful. Often the question arises whether conformations obtained in this way appropriately reflect the reactivity of enzymes, however. Rates that characterize transitions are therefore compulsory experiments for the elucidation of the reaction mechanism. Such experiments have been performed for the reverse transcriptase of the type 1 human immunodeficiency virus (HIV-1 RT). RESULTS: We have developed a methodology to monitor the interplay between HIV-1 RT and its DNA substrate. To probe the protein-DNA interactions, the sugar backbone of one nucleotide was modified by a substituent that influenced the efficiency of the chain elongation in a characteristic way. We found that strand elongation after incorporation of the modified nucleotide follows a discontinuous efficiency for the first four nucleotides. The reaction efficiencies could be correlated with the distance between the sugar substituent and the enzyme. The model was confirmed by kinetic experiments with HIV-1 RT mutants. CONCLUSIONS: Experiments with HIV-1 RT demonstrate that strand-elongation efficiency using a modified nucleotide correlates well with distances between the DNA substrate and the enzyme. The functional group at the modified nucleotides acts as an 'antenna' for steric interactions that changes the optimal transition state. Kinetic experiments in combination with backbone-modified nucleotides can therefore be used to gain structural information about reverse transcriptases and DNA polymerases.  (+info)

High-throughput screening of small molecules in miniaturized mammalian cell-based assays involving post-translational modifications. (4/6505)

BACKGROUND: Fully adapting a forward genetic approach to mammalian systems requires efficient methods to alter systematically gene products without prior knowledge of gene sequences, while allowing for the subsequent characterization of these alterations. Ideally, these methods would also allow function to be altered in a temporally controlled manner. RESULTS: We report the development of a miniaturized cell-based assay format that enables a genetic-like approach to understanding cellular pathways in mammalian systems using small molecules, rather than mutations, as the source of gene-product alterations. This whole-cell immunodetection assay can sensitively detect changes in specific cellular macromolecules in high-density arrays of mammalian cells. Furthermore, it is compatible with screening large numbers of small molecules in nanoliter to microliter culture volumes. We refer to this assay format as a 'cytoblot', and demonstrate the use of cytoblotting to monitor biosynthetic processes such as DNA synthesis, and post-translational processes such as acetylation and phosphorylation. Finally, we demonstrate the applicability of these assays to natural-product screening through the identification of marine sponge extracts exhibiting genotype-specific inhibition of 5-bromodeoxyuridine incorporation and suppression of the anti-proliferative effect of rapamycin. CONCLUSIONS: We show that cytoblots can be used for high-throughput screening of small molecules in cell-based assays. Together with small-molecule libraries, the cytoblot assay can be used to perform chemical genetic screens analogous to those used in classical genetics and thus should be applicable to understanding a wide variety of cellular processes, especially those involving post-transitional modifications.  (+info)

A novel H2A/H4 nucleosomal histone acetyltransferase in Tetrahymena thermophila. (5/6505)

Recently, we reported the identification of a 55-kDa polypeptide (p55) from Tetrahymena macronuclei as a catalytic subunit of a transcription-associated histone acetyltransferase (HAT A). Extensive homology between p55 and Gcn5p, a component of the SAGA and ADA transcriptional coactivator complexes in budding yeast, suggests an immediate link between the regulation of chromatin structure and transcriptional output. Here we report the characterization of a second transcription-associated HAT activity from Tetrahymena macronuclei. This novel activity is distinct from complexes containing p55 and putative ciliate SAGA and ADA components and shares several characteristics with NuA4 (for nucleosomal H2A/H4), a 1.8-MDa, Gcn5p-independent HAT complex recently described in yeast. A key feature of both the NuA4 and Tetrahymena activities is their acetylation site specificity for lysines 5, 8, 12, and 16 of H4 and lysines 5 and 9 of H2A in nucleosomal substrates, patterns that are distinct from those of known Gcn5p family members. Moreover, like NuA4, the Tetrahymena activity is capable of activating transcription from nucleosomal templates in vitro in an acetyl coenzyme A-dependent fashion. Unlike NuA4, however, sucrose gradient analyses of the ciliate enzyme, following sequential denaturation and renaturation, estimate the molecular size of the catalytically active subunit to be approximately 80 kDa, consistent with the notion that a single polypeptide or a stable subcomplex is sufficient for this H2A/H4 nucleosomal HAT activity. Together, these data document the importance of this novel HAT activity for transcriptional activation from chromatin templates and suggest that a second catalytic HAT subunit, in addition to p55/Gcn5p, is conserved between yeast and Tetrahymena.  (+info)

Virus infection leads to localized hyperacetylation of histones H3 and H4 at the IFN-beta promoter. (6/6505)

Transcriptional activation of the human interferon-beta (IFN-beta) gene by virus infection requires the assembly of a higher order nucleoprotein complex, the enhanceosome, which consists of the transcriptional activators NF-kappa B (p50/p65), ATF-2/c-jun, IRF-3 and IRF-7, architectural protein HMGI(Y), and the coactivators p300 and CBP. In this report, we show that virus infection of cells results in a dramatic hyperacetylation of histones H3 and H4 that is localized to the IFN-beta promoter. Furthermore, expressing a truncated version of IRF-3, which lacks a p300/CBP interaction domain, suppresses both histone hyperacetylation and activation of the IFN-beta gene. Thus, coactivator-mediated localized hyperacetylation of histones may play a crucial role in inducible gene expression.  (+info)

Gibberellic acid stabilises microtubules in maize suspension cells to cold and stimulates acetylation of alpha-tubulin. (7/6505)

Gibberellic acid is known to stabilise microtubules in plant organs against depolymerisation. We have now devised a simplified cell system for studying this. Pretreatment of a maize cell suspension with gibberellic acid for just 3 h stabilised protoplast microtubules against depolymerisation on ice. In other eukaryotes, acetylation of alpha-tubulin is known to correlate with microtubule stabilisation but this is not established in plants. By isolating the polymeric tubulin fraction from maize cytoskeletons and immunoblotting with the antibody 6-11B-1, we have demonstrated that gibberellic acid stimulates the acetylation of alpha-tubulin. This is the first demonstrated link between microtubule stabilisation and tubulin acetylation in higher plants.  (+info)

Expanded lysine acetylation specificity of Gcn5 in native complexes. (8/6505)

The coactivator/adaptor protein Gcn5 is a conserved histone acetyltransferase, which functions as the catalytic subunit in multiple yeast transcriptional regulatory complexes. The ability of Gcn5 to acetylate nucleosomal histones is significantly reduced relative to its activity on free histones, where it predominantly modifies histone H3 at lysine 14. However, the association of Gcn5 in multisubunit complexes potentiates its nucleosomal histone acetyltransferase activity. Here, we show that the association of Gcn5 with other proteins in two native yeast complexes, Ada and SAGA (Spt-Ada-Gcn5-acetyltransferase), directly confers upon Gcn5 the ability to acetylate an expanded set of lysines on H3. Furthermore Ada and SAGA have overlapping, yet distinct, patterns of acetylation, suggesting that the association of specific subunits determines site specificity.  (+info)

Plus itPlus it
Global alterations in histone acetylation levels have been observed in both normal and cancer cells and can be prognostic of clinical outcome. However, unlike site-specific acetylation changes that can affect transcription of particular genes, the reason for genome-wide changes has been less clear. Because acetyl-coA molecules required for histone acetylation and acetate anions generated by histone deacetylation are required for many metabolic processes, McBrian and colleagues hypothesized that metabolic or physiologic cues might affect global histone acetylation levels. Systematic testing of the effects of culture medium components on histone acetylation revealed that decreased sodium bicarbonate concentrations resulting in lowered extracellular and intracellular pH led to a rapid, marked reduction in total levels of histone H3 and H4 acetylation at multiple lysine residues. These pH-dependent changes were specific to histone acetylation, as histone methylation was unaffected and required ...
https://cancerdiscovery.aacrjournals.org/content/3/1/12.1
IJMS | Free Full-Text | The Effect of Hydroxamic Siderophores Structure on Acetylation of Histone H3 and Alpha Tubulin in...IJMS | Free Full-Text | The Effect of Hydroxamic Siderophores Structure on Acetylation of Histone H3 and Alpha Tubulin in...
Protein acetylation affects gene expression, as well as other processes in cells, and it might be dependent on the availability of the metals. However, whether iron chelating compounds (siderophores) can have an effect on the acetylation process in plant roots is largely unknown. In the present study, western blotting and confocal microscopy was used to examine the degree of acetylation of histone H3 and alpha tubulin in Pinus sylvestris root cells in the presence of structurally different siderophores. The effect of metabolites that were produced by pathogenic and mycorrhizal fungi was also assessed. No effect was observed on histone acetylation. By contrast, the metabolites of the pathogenic fungus were able to decrease the level of microtubule acetylation, whereas treatment with iron-free ferrioxamine (DFO) was able to increase it. This latter was not observed when ferrioxamine-iron complexes were used. The pathogen metabolites induced important modifications of cytoskeleton organization.
https://www.mdpi.com/1422-0067/20/23/6099/htm
Frontiers | Dynamic Protein Acetylation in Plant-Pathogen Interactions | Plant ScienceFrontiers | Dynamic Protein Acetylation in Plant-Pathogen Interactions | Plant Science
Pathogen infection triggers complex molecular perturbations within host cells that results in either resistance or susceptibility. Protein acetylation is an emerging biochemical modification that appears to play central roles during host-pathogen interactions. To date, research in this area has focused on two main themes linking protein acetylation to plant immune signaling. Firstly, it has been established that proper gene expression during defense responses requires modulation of histone acetylation within target gene promoter regions. Second, some pathogens can deliver effector molecules that encode acetyltransferases directly within the host cell to modify acetylation of specific host proteins. Collectively these findings suggest that the acetylation level for a range of host proteins may be modulated to alter the outcome of pathogen infection. This review will focus on summarizing our current understanding of the roles of protein acetylation in plant defense and highlight the utility of proteomics
https://www.frontiersin.org/articles/10.3389/fpls.2016.00421/full
Systematic analysis of human lysine acetylation proteins and accurate prediction of human lysine acetylation through bi...Systematic analysis of human lysine acetylation proteins and accurate prediction of human lysine acetylation through bi...
AbstractLysine acetylation is a reversible post-translational modification (PTM) which has been linked to many biological and pathological implications. Hence, localization of lysine acetylation is essential for deciphering the mechanism of such implications. Whereas many acetylated lysines in human proteins have been localized through experimental approaches in wet lab, it still fails to reach completion. In the present study, we proposed a novel feature extraction approach, bi-relative adapted binomial score Bayes (BRABSB), combined with support vector machines (SVMs) to construct a human-specific lysine acetylation predictor, which yields, on average, a sensitivity of 83.91%, a specificity of 87.25% and an accuracy of 85.58%, in the case of 5-fold cross validation experiments. Results obtained through the validation on independent data sets show that the proposed approach here outperforms other existing lysine acetylation predictors. Furthermore, due to the fact that global analysis of human ...
https://aims.cuhk.edu.hk/converis/portal/detail/Publication/19522465?auxfun=&lang=en_GB
Proteome-Wide Mapping of the Drosophila Acetylome Demonstrates a High Degree of Conservation of Lysine Acetylation | Science...Proteome-Wide Mapping of the Drosophila Acetylome Demonstrates a High Degree of Conservation of Lysine Acetylation | Science...
Posttranslational modification of proteins by acetylation and phosphorylation regulates most cellular processes in living organisms. Surprisingly, the evolutionary conservation of phosphorylated serine and threonine residues is only marginally higher than that of unmodified serines and threonines. With high-resolution mass spectrometry, we identified 1981 lysine acetylation sites in the proteome of Drosophila melanogaster. We used data sets of experimentally identified acetylation and phosphorylation sites in Drosophila and humans to analyze the evolutionary conservation of these modification sites between flies and humans. Site-level conservation analysis revealed that acetylation sites are highly conserved, significantly more so than phosphorylation sites. Furthermore, comparison of lysine conservation in Drosophila and humans with that in nematodes and zebrafish revealed that acetylated lysines were significantly more conserved than were nonacetylated lysines. Bioinformatics analysis using ...
http://stke.sciencemag.org/content/4/183/ra48
Aims To research the hypothesis that alteration in histone acetylation/deacetylation sets - CFTR cystic fibrosis transmembrane...Aims To research the hypothesis that alteration in histone acetylation/deacetylation sets - CFTR cystic fibrosis transmembrane...
Aims To research the hypothesis that alteration in histone acetylation/deacetylation sets off aberrant STAT1/MyD88 appearance in macrophages from diabetics. promoters in macrophages from T1D mice and AA in vitro treatment decreased STAT1 and MyD88 mRNA appearance. Conclusions/interpretation These outcomes suggest that histone acetylation drives raised Stat1/Myd88 appearance in macrophages from diabetic mice, which mechanism could be involved with sterile irritation and diabetes comorbidities. (A) and (B) mRNA appearance had been dependant on qPCR, and MyD88, STAT1, iNOS and actin proteins appearance had been motivated after 6 times of lifestyle by immunoblotting (C). Blots proven are in one consultant experiment (n=3). Bone tissue marrow-derived macrophages from non-diabetic and T1D mice had been differentiated in 5 mM or 25 mM of blood sugar, and (D) and (E) appearance was dependant on qPCR. Bone tissue marrow-derived macrophages from non-diabetic had been differentiated in low 5 mM or 25 mM of ...
http://www.morainetownshipdems.org/2019/03/02/aims-to-research-the-hypothesis-that-alteration-in-histone-acetylationdeacetylation-sets/
ABL1 | Vascular Dysfunction Induced in Offspring by Maternal Dietary FatABL1 | Vascular Dysfunction Induced in Offspring by Maternal Dietary Fat
Reversible acetylation of -tubulin can be an evolutionarily conserved modification in microtubule networks. SP1-reliant IL-10 transcription. Amazingly, the augmented p38 signaling is definitely suppressed by MEC17 inactivation. Our results determine reversible microtubule acetylation like a kinase signaling modulator and an essential component in the inflammatory response. Intro Acetylation on lysine (K) 40 of -tubulin can be an evolutionarily conserved changes managed from the acetyltransferase MEC17 (also termed TAT1)1, 2 as well as the deacetylase HDAC63, 4. The prevalence and extremely enriched distribution of -tubulin acetylation in the microtubule network suggests a simple function of the changes5, 6. 488-81-3 manufacture Remarkably, mice missing MEC17 or HDAC6 are grossly regular despite an extraordinary perturbation in microtubule acetylation7C10. -tubulin acetylation on K40 can be dispensable in Tetrahymena11. Hence under laboratory circumstances, microtubule acetylation is certainly ...
http://sc-26196.com/tag/abl1/
Site-specific determination of lysine acetylation stoichiometries on the proteome-scale<...Site-specific determination of lysine acetylation stoichiometries on the proteome-scale<...
TY - CHAP. T1 - Site-specific determination of lysine acetylation stoichiometries on the proteome-scale. AU - Chen, Yue. AU - Li, Yunan. PY - 2019. Y1 - 2019. N2 - Posttranslational modification of proteins (PTMs) offers a versatile mechanism to fine-tune the structure, activity, and interactions of the target proteins. Understanding the dynamics and prevalence of the PTM at the site-specific level will provide mechanistic insight into the physiological significance of the modification pathway in cells and diseases. In this chapter, we describe a highly efficient chemical proteomic workflow for the absolute quantification of lysine acetylation stoichiometry. The strategy is capable of measuring the site-specific prevalence of acetylation in a system-wide and untargeted manner. We highlight the importance of validating the workflow using standard proteins and synthetic peptides. Detailed protocols for global stoichiometric analysis of lysine acetylation from cell lysate are presented.. AB - ...
https://experts.umn.edu/en/publications/site-specific-determination-of-lysine-acetylation-stoichiometries
Other Histone Acetylation Antibodies | EpiGentekOther Histone Acetylation Antibodies | EpiGentek
The process of histone acetylation at lysine residues by histone acetyltransferase (HAT) is an important epigenetic marker and can be measured with the use of histone lysine acetylation antibodies . Acetylation of histones...
https://www.epigentek.com/catalog/histone-lysine-acetylation-antibodies-c-35_104_30_127.html?currency=ww
Acetylation of retinal histones in diabetes increases inflammatory proteins: Effects of minocycline and manipulation of histone...
TY - JOUR. T1 - Acetylation of retinal histones in diabetes increases inflammatory proteins. T2 - Effects of minocycline and manipulation of histone acetyltransferase (HAT) and histone deacetylase (HDAC). AU - Kadiyala, Chandra Sekhar Rao. AU - Zheng, Ling. AU - Du, Yunpeng. AU - Yohannes, Elizabeth. AU - Kao, Hung Ying. AU - Miyagi, Masaru. AU - Kern, Timothy S.. PY - 2012/7/27. Y1 - 2012/7/27. N2 - Histone acetylation was significantly increased in retinas from diabetic rats, and this acetylation was inhibited in diabetics treated with minocycline, a drug known to inhibit early diabetic retinopathy in animals. Histone acetylation and expression of inflammatory proteins that have been implicated in the pathogenesis of diabetic retinopathy were increased likewise in cultured retinal Müller glia grown in a diabetes-like concentration of glucose. Both the acetylation and induction of the inflammatory proteins in elevated glucose levels were significantly inhibited by inhibitors of histone ...
https://researchoutput.ncku.edu.tw/en/publications/acetylation-of-retinal-histones-in-diabetes-increases-inflammator
New JBC paper on histone acetylation - Integrative Biomolecular ModelingNew JBC paper on histone acetylation - Integrative Biomolecular Modeling
Our collaboration with the group of Prof. Zheng on histone acetylation has led to a combined experimental/computational paper available from the Journal of Biological Chemistry.
https://ivanov-group.org/2016/05/08/new-jbc-paper-on-histone-acetylation/
Histone H4 acetylation and replication timing in Chinese hamster chromosomes. - Semantic ScholarHistone H4 acetylation and replication timing in Chinese hamster chromosomes. - Semantic Scholar
The distribution of acetylated isoforms of histone H4 along Chinese hamster chromosomes has been studied by immunostaining with antibodies recognizing H4 acetylated at defined lysines in its N-terminal domain. The heterochromatic long arm of the X chromosome in both female (CHO) and male (DON) cell lines is underacetylated at three out of four lysines (5, 8, and 12). In contrast, the level of acetylation at lysine 16, which is the first to be acetylated in mammals, was similar in X chromosomes and autosomes. Labeling of the cells with bromodeoxyuridine (BrdU) to mark late-replicating chromosome domains, followed by double immunostaining with antibodies to BrdU and acetylated H4, showed a close, though not perfect, correlation between late replication and low levels of H4 acetylation. The results show that levels of histone acetylation are associated with the replication timing of defined domains on both the X chromosome and autosomes, but the exceptions we observe suggest that this link is not absolute
https://www.semanticscholar.org/paper/Histone-H4-acetylation-and-replication-timing-in-C-Belyaev-Keohane/3686fca1d845fbb586b94d3467780f565687368a
Conservation of deposition-related acetylation sites in newly synthesized histones H3 and H4 | PNASConservation of deposition-related acetylation sites in newly synthesized histones H3 and H4 | PNAS
Newly synthesized histone H4 is deposited in a diacetylated isoform in a wide variety of organisms. In Tetrahymena a specific pair of residues, lysines 4 and 11, have been shown to undergo this modification in vivo. In this report, we demonstrate that the analogous residues, lysines 5 and 12, are acetylated in Drosophila and HeLa H4. These data strongly suggest that deposition-related acetylation sites in H4 have been highly, perhaps absolutely, conserved. In Tetrahymena and Drosophila newly synthesized histone H3 is also deposited in several modified forms. Using pulse-labeled H3 we have determined that, like H4, a specific, but distinct, subset of lysines is acetylated in these organisms. In Tetrahymena, lysines 9 and 14 are highly preferred sites of acetylation in new H3 while in Drosophila, lysines 14 and 23 are strongly preferred. No evidence has been obtained for acetylation of newly synthesized H3 in HeLa cells. Thus, although the pattern and sites of deposition-related acetylation appear ...
https://www.pnas.org/content/92/4/1237?ijkey=a32f0f38b993cf9462a2e99068710556a2a3d151&keytype2=tf_ipsecsha
Analysis of acetylation stoichiometry suggests that SIRT3 repairs nonenzymatic acetylation lesions | The EMBO Journal
Many studies have shown that SIRT3 deficiency results in increased mitochondrial acetylation and reduced activity of numerous mitochondrial enzymes (Newman et al, 2012). SIRT3 protein levels are increased upon fasting and calorie restriction (Shi et al, 2005; Palacios et al, 2009; Hirschey et al, 2010; Tao et al, 2010; Newman et al, 2012), and increased SIRT3 activity has been suggested to regulate metabolism under these conditions. However, with the exception of a single study (Fan et al, 2014), a regulatory axis between enzyme‐catalyzed acetylation and SIRT3‐mediated deacetylation has not been demonstrated. A hallmark of protein regulation by posttranslational modifications is site‐specific, enzyme‐catalyzed modification that mostly occurs in a conditional manner. However, there is little evidence of enzyme‐catalyzed, site‐specific acetylation in mitochondria, and several studies suggest that most mitochondrial acetylation occurs nonenzymatically (Wagner & Payne, 2013; Pougovkina ...
http://emboj.embopress.org/content/early/2015/09/09/embj.201591271
Histone acetylation: molecular mnemonics on the chromatin - Tsai Laboratory
Long-lasting memories require specific gene expression programmes that are, in part, orchestrated by epigenetic mechanisms. Of the epigenetic modifications identified in cognitive processes, histone acetylation has spurred considerable interest. Whereas increments in histone acetylation have consistently been shown to favour learning and memory, a lack thereof has been causally implicated in cognitive impairments in neurodevelopmental disorders, neurodegeneration and ageing. As histone acetylation and cognitive functions can be pharmacologically restored by histone deacetylase inhibitors, this epigenetic modification might constitute a molecular memory aid on the chromatin and, by extension, a new template for therapeutic interventions against cognitive frailty.. Read more → ...
http://tsailaboratory.mit.edu/2013/02/28/histone-acetylation-molecular-mnemonics-chromatin/
WERAM - Writers, Erasers & Readers of Histone Acetylation and Methylation Database
Histone acetylation is a hallmark of chromatin that has an open structure that can be accessed by DNA and RNA polymerases as well as transcription factors, resulting in the activation of gene transcription (Filippakopoulos and Knapp, 2014). Correspondingly, histone methylation increases the basicity and hydrophobicity of histone tails and the affinity of certain proteins, such as transcription factors, toward DNA (Teperino et al., 2010), thus affecting the gene expression. In this database, we have collected 584 non-redundant protein data of 8 organisms including H. sapiens, M. musculus, R. norvegicus, D. melanogaster, C. elegans, A. thaliana, S. pombe and S. cerevisiae from the literature. The data are further classified into 15 families for histone acetylation writers, erasers and readers and 32 families for histone methylation writers, erasers and readers, respectively. WERAM 1.0 is a comprehensive Eukaryotic Writers, Erasers and Readers protein of Histone Acetylation and Methylation system ...
http://weram.biocuckoo.org/index.php
Enhancement of lysine acetylation accelerates wound repair<...
TY - JOUR. T1 - Enhancement of lysine acetylation accelerates wound repair. AU - Spallotta, Francesco. AU - Cencioni, Chiara. AU - Straino, Stefania. AU - Sbardella, Gianluca. AU - Castellano, Sabrina. AU - Capogrossi, Maurizio C.. AU - Martelli, Fabio. AU - Gaetano, Carlo. PY - 2013. Y1 - 2013. N2 - In physiopathological conditions, such as diabetes, wound healing is significantly compromised and chronic complications, including ulcers, may occur. In a mouse model of skin repair, we recently reported that wound treatment with Sirtuin activators and class I HDAC inhibitors induced keratinocyte proliferation and enhanced healing via a nitric oxide (NO) dependent mechanism. We observed an increase in total protein acetylation in the wound area, as determined by acetylation of a-tubulin and histone H3 Lysine 9. We reasoned that this process activated cell function as well as regulated gene expression to foster tissue repair. We report here that the direct activation of P300/CBP-associated factor ...
https://moh-it.pure.elsevier.com/en/publications/enhancement-of-lysine-acetylation-accelerates-wound-repair
Acetylation of Rb by PCAF is required for nuclear localization and keratinocyte differentiation | Journal of Cell ScienceAcetylation of Rb by PCAF is required for nuclear localization and keratinocyte differentiation | Journal of Cell Science
This study shows that Rb is a key regulator of differentiation and that acetylation is an important modification during this process. We have investigated the role of Rb acetylation in keratinocyte differentiation by mutating the major acetylation sites, lysines 873 and 874, to arginine and then determining the ability of the mutant to restore differentiation in Rb-knockdown keratinocytes. Mutation of the acetylation sites did not affect the ability of Rb to inhibit the proliferation, probably because of the fact that RbRR can interact with and inhibit E2F1 (Markham et al., 2006). This also suggests that inhibition of E2F family members is not sufficient to induce terminal differentiation, as has previously reported in the Saos-2 cell line (Sellers et al., 1998). However, unlike wild-type Rb, the acetylation mutant is unable to induce either early or late differentiation markers and, because Rb is acetylated relatively late during differentiation, this suggests that either the early events are ...
http://jcs.biologists.org/content/123/21/3718
WERAM - Writers, Erasers & Readers of Histone Acetylation and Methylation Database
1. Acetylation Databases. (1) PhosphoSitePlus: (PSP) is a comprehensive, manually curated and interactive resource on post-translational modifications (PTM). PSP contains encompasses 130000 non-redundant modification sites, manily on phosphorylation, ubiquitinylation and acetylation (Hornbeck, et al., 2004). (2) g2pDB: A Database Mapping Protein Post-Translational Modifications to Genomic Coordinates. The original data comes mainly from published studies, many of which involve the investigation of post-translational modification acceptor site assignments, e.g., phosphorylation, ubiquitination, SUMOylation, acetylation, and N-linked glycosylation sites. (Keegan S, et al., 2016). (3) dbPTM 2.0: integrates experimentally verified PTMs from several databases, and to annotate the predicted PTMs on Swiss-Prot proteins , 2,071 acetylation sites were included while most of which were N-alpha-terminal ones (Lee TY, et al., 2006) . (4) HPRD release 9: HPRD currently contains information for 16,972 PTMs ...
http://weram.biocuckoo.org/link.php
Search Results | Journal of Cell Biology | Rockefeller University PressSearch Results | Journal of Cell Biology | Rockefeller University Press
Lysine acetylation is an important posttranslational modification that regulates microtubules and microfilaments, but its effects on intermediate filament proteins (IFs) are unknown. We investigated the regulation of keratin 8 (K8), a type II simple epithelial IF, by lysine acetylation. K8 was basally acetylated and the highly conserved Lys-207 was a major acetylation site. K8 acetylation regulated filament organization and decreased keratin solubility. Acetylation of K8 was rapidly responsive to changes in glucose levels and was up-regulated in response to nicotinamide adenine dinucleotide (NAD) depletion and in diabetic mouse and human livers. The NAD-dependent deacetylase sirtuin 2 (SIRT2) associated with and deacetylated K8. Pharmacologic or genetic inhibition of SIRT2 decreased K8 solubility and affected filament organization. Inhibition of K8 Lys-207 acetylation resulted in site-specific phosphorylation changes of K8. Therefore, K8 acetylation at Lys-207, a highly conserved residue among ...
https://rupress.org/jcb/search-results?f_Authors=Raymond+Kwan
Metabolic control of methylation and acetylation<...Metabolic control of methylation and acetylation<...
TY - JOUR. T1 - Metabolic control of methylation and acetylation. AU - Su, Xiaoyang. AU - Wellen, Kathryn E.. AU - Rabinowitz, Joshua D.. PY - 2016/2/1. Y1 - 2016/2/1. N2 - Methylation and acetylation of DNA and histone proteins are the chemical basis for epigenetics. From bacteria to humans, methylation and acetylation are sensitive to cellular metabolic status. Modification rates depend on the availability of one-carbon and two-carbon substrates (S-adenosylmethionine, acetyl-CoA, and in bacteria also acetyl-phosphate). In addition, they are sensitive to demodification enzyme cofactors (α-ketoglutarate, NAD+) and structural analog metabolites that function as epigenetic enzyme inhibitors (e.g., S-adenosylhomocysteine, 2-hydroxyglutarate). Methylation and acetylation likely initially evolved to tailor protein activities in microbes to their metabolic milieu. While the extracellular environment of mammals is more tightly controlled, the combined impact of nutrient abundance and metabolic enzyme ...
https://www.researchwithrutgers.com/en/publications/metabolic-control-of-methylation-and-acetylation
Protein Lounge: Protein Acetylation and DeacetylationProtein Lounge: Protein Acetylation and Deacetylation
|P>Eukaryotic transcription is a highly regulated process, and acetylation plays a major role in this regulation. Acetylation can occur on histones, DNA-binding TF (Transcription Factors), acetylases, nuclear import factors, non-nuclear proteins (Alpha-tubulin) and proteins that shuttle from the nucleus to [...]
http://www.proteinlounge.com/Pathway/Protein%20Acetylation%20and%20Deacetylation
Antibodies to Modifying Enzymes/Histone Deacetylase - Antibodies in Chromatin Remodeling | Sigma-AldrichAntibodies to Modifying Enzymes/Histone Deacetylase - Antibodies in Chromatin Remodeling | Sigma-Aldrich
Regulation of gene expression is mediated by several mechanisms including DNA methylation, ATP-dependent chromatin remodeling, and posttranslational modifications of histones. One of the major modifications of histones consists of the dynamic acetylation and deacetylation of ε-amino groups of lysine residues present in the tail of core histones.1 The enzymes responsible for this reversible acetylation/deacetylation process are histone acetyltransferases (HATs) and histone deacetylases (HDACs), respectively.
https://www.sigmaaldrich.com/life-science/cell-biology/antibodies/antibody-products.html?TablePage=18824718
IJMS | Free Full-Text | DNA Damage Induced MutS Homologue hMSH4 AcetylationIJMS | Free Full-Text | DNA Damage Induced MutS Homologue hMSH4 Acetylation
Acetylation of non-histone proteins is increasingly recognized as an important post-translational modification for controlling the actions of various cellular processes including DNA repair and damage response. Here, we report that the human MutS homologue hMSH4 undergoes acetylation following DNA damage induced by ionizing radiation (IR). To determine which acetyltransferases are responsible for hMSH4 acetylation in response to DNA damage, potential interactions of hMSH4 with hTip60, hGCN5, and hMof were analyzed. The results of these experiments indicate that only hMof interacts with hMSH4 in a DNA damage-dependent manner. Intriguingly, the interplay between hMSH4 and hMof manipulates the outcomes of nonhomologous end joining (NHEJ)-mediated DNA double strand break (DSB) repair and thereby controls cell survival in response to IR. This study also shows that hMSH4 interacts with HDAC3, by which HDAC3 negatively regulates the levels of hMSH4 acetylation. Interestingly, elevated levels of HDAC3 correlate
https://www.mdpi.com/1422-0067/14/10/20966
Identification and specificities of N‐terminal acetyltransferases from Saccharomyces cerevisiae | The EMBO Journal
The two cotranslational processes, cleavage of N‐terminal methionine residues and N‐terminal acetylation, are by far the most common modifications, occurring on the vast majority of proteins. Proteins from prokaryotes, mitochondria and chloroplasts initiate with formylmethionine, whereas proteins from the cytosol of eukaryotes initiate with methionine. The formyl group is removed from prokaryotic proteins by a deformylase, resulting in methionine at the N‐termini. The methionine at the N‐termini is cleaved from nascent chains of most prokaryotic and eukaryotic proteins. N‐terminal acetylation occurs subsequently on certain of the proteins, either containing or lacking the methionine residue. This N‐terminal acetylation occurs on more than one‐half of eukaryotic proteins, but seldom on prokaryotic proteins (Driessen et al., 1985; Kendall et al., 1990).. Because the N‐terminal region of yeast iso‐1‐cytochrome c (iso‐1) is dispensable for biosynthesis, function and ...
http://emboj.embopress.org/content/18/21/6155
The structural basis of protein acetylation by the p300/CBP transcriptional coactivator<...
TY - JOUR. T1 - The structural basis of protein acetylation by the p300/CBP transcriptional coactivator. AU - Liu, Xin. AU - Wang, Ling. AU - Zhao, Kehao. AU - Thompson, Paul R.. AU - Hwang, Yousang. AU - Marmorstein, Ronen. AU - Cole, Philip A.. PY - 2008/2/14. Y1 - 2008/2/14. N2 - The transcriptional coactivator p300/CBP (CREBBP) is a histone acetyltransferase (HAT) that regulates gene expression by acetylating histones and other transcription factors. Dysregulation of p300/CBP HAT activity contributes to various diseases including cancer. Sequence alignments, enzymology experiments and inhibitor studies on p300/CBP have led to contradictory results about its catalytic mechanism and its structural relation to the Gcn5/PCAF and MYST HATs. Here we describe a high-resolution X-ray crystal structure of a semi-synthetic heterodimeric p300 HAT domain in complex with a bi-substrate inhibitor, Lys-CoA. This structure shows that p300/CBP is a distant cousin of other structurally characterized HATs, but ...
https://utsouthwestern.pure.elsevier.com/en/publications/the-structural-basis-of-protein-acetylation-by-the-p300cbp-transc
Histone acetyltransferases interact with and acetylate p70 ribosomal S6 kinases in vitro and in vivoHistone acetyltransferases interact with and acetylate p70 ribosomal S6 kinases in vitro and in vivo
The 70kDa ribosomal protein S6 kinases (S6K1 and S6K2) play important roles in the regulation of protein synthesis, cell growth and survival. S6Ks are activated in response to mitogen stimulation and nutrient sufficiency by the phosphorylation of conserved serine and threonine residues. Here we show for the first time, that in addition to phosphorylation, S6Ks are also targeted by lysine acetylation. Following mitogen stimulation, S6Ks interact with the p300 and p300/CBP-associated factor (PCAF) acetyltransferases. S6Ks can be acetylated by p300 and PCAF in vitro and S6K acetylation is detected in cells expressing p300. Furthermore, it appears that the acetylation sites targeted by p300 lie within the divergent C-terminal regulatory domains of both S6K1 and S6K2. Acetylation of S6K1 and 2 is increased upon the inhibition of class I/II histone deacetylases (HDACs) by trichostatin-A, while the enhancement of S6K1 acetylation by nicotinamide suggests the additional involvement of sirtuin ...
http://uu.diva-portal.org/smash/record.jsf?pid=diva2:295218
Acetylation distributed by Caltag Medsystems LtdAcetylation distributed by Caltag Medsystems Ltd
Caltag Medsystems provide a range of kits to study histone acetylation and histone deacetylation which is involved the addition or removal of an acetyl group on lysine residues in the N-terminal tail and on the surface of the nucelosome core of histone proteins. Acetylated and deacetylated histones are considered epigenetic tags within chromatin by relaxing (euchromatin) or tightening (heterochromatin) chromatin structure, subsequently increasing or decreasing gene transcription levels. Kits are available for Histone Acetylation Quantification, Histone Acetyltransferase (HAT) Assays, and Histone Deacetylase (HDAC) Assays.
https://www.caltagmedsystems.co.uk/pricing_ordering/products_ordering.php?CG_ID=41
Nucleosome structure incorporated histone acetylation site prediction in arabidopsis thaliana - pdf descargar
Nucleosome structure incorporated histone acetylation site prediction in arabidopsis thaliana. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
http://libros.duhnnae.com/2017/jul6/15002385545-Nucleosome-structure-incorporated-histone-acetylation-site-prediction-in-arabidopsis-thaliana.php
Regulation of histone acetylation and nucleosome assembly by transcription factor JDP2 | Nature Structural & Molecular BiologyRegulation of histone acetylation and nucleosome assembly by transcription factor JDP2 | Nature Structural & Molecular Biology
Jun dimerization protein-2 (JDP2) is a component of the AP-1 transcription factor that represses transactivation mediated by the Jun family of proteins. Here, we examine the functional mechanisms of JDP2 and show that it can inhibit p300-mediated acetylation of core histones in vitro and in vivo. Inhibition of histone acetylation requires the N-terminal 35 residues and the DNA-binding region of JDP2. In addition, we demonstrate that JDP2 has histone-chaperone activity in vitro. These results suggest that the sequence-specific DNA-binding protein JDP2 may control transcription via direct regulation of the modification of histones and the assembly of chromatin.
https://www.nature.com/articles/nsmb1063?error=cookies_not_supported&code=459b0c68-4bb1-4af3-95b1-84c46d0ba21b
H3K9 and H3K14 acetylation co-occur at many gene regulatory elements, while H3K14ac marks a subset of inactive inducible...H3K9 and H3K14 acetylation co-occur at many gene regulatory elements, while H3K14ac marks a subset of inactive inducible...
Transcription regulation in pluripotent embryonic stem (ES) cells is a complex process that involves multitude of regulatory layers, one of which is post-translational modification of histones. Acetylation of specific lysine residues of histones plays a key role in regulating gene expression. Here we have investigated the genome-wide occurrence of two histone marks, acetylation of histone H3K9 and K14 (H3K9ac and H3K14ac), in mouse embryonic stem (mES) cells. Genome-wide H3K9ac and H3K14ac show very high correlation between each other as well as with other histone marks (such as H3K4me3) suggesting a coordinated regulation of active histone marks. Moreover, the levels of H3K9ac and H3K14ac directly correlate with the CpG content of the promoters attesting the importance of sequences underlying the specifically modified nucleosomes. Our data provide evidence that H3K9ac and H3K14ac are also present over the previously described bivalent promoters, along with H3K4me3 and H3K27me3. Furthermore, like
https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-13-424
Lysine Acetylation Controls Local Protein Conformation by Influencing Proline Isomerization - Oxford Neuroscience
© 2014 The Authors. Gene transcription responds to stress and metabolic signals to optimize growth and survival. Histone H3 (H3) lysine 4 trimethylation (K4me3) facilitates state changes, but how levels are coordinated with the environment is unclear. Here, we show that isomerization of H3 at the alanine 15-proline 16 (A15-P16) peptide bond is influenced by lysine 14 (K14) and controls gene-specific K4me3 by balancing the actions of Jhd2, the K4me3 demethylase, and Spp1, a subunit of the Set1 K4 methyltransferase complex. Acetylation at K14 favors the A15-P16. trans conformation and reduces K4me3. Environmental stress-induced genes are most sensitive to the changes atK14 influencing H3 tail conformation and K4me3. By contrast, ribosomal protein genes maintain K4me3, required for their repression during stress, independently of Spp1, K14, and P16. Thus, the plasticity in control of K4me3, via signaling to K14 and isomerization at P16, informs distinct gene regulatory mechanisms and processes involving
https://www.neuroscience.ox.ac.uk/publications/506203
Protein acetylation in prokaryotesProtein acetylation in prokaryotes
Protein acetylation plays a critical regulatory role in eukaryotes but until recently its significance and function in bacteria and the archaea were obscure. It is now clear, however, that prokaryotes have the capacity to acetylate both the α-amino groups of N-terminal residues and the ε-amino group …
https://pubmed.ncbi.nlm.nih.gov/21674803/?dopt=Abstract
Histone Acetylation Proteins | EpiGentekHistone Acetylation Proteins | EpiGentek
Histone acetyltransferase (HATs) proteins are involved in histone acetylation, or the addition of an acetyl group to lysine residues in the N-terminal tail and on the surface of the nucelosome core of histone proteins. They...
https://www.epigentek.com/catalog/histone-acetylation-proteins-c-76_102_88.html?currency=ww
Changes to DNA on-off switches affect cells ability to repair breaks, respond to chemotherapyChanges to DNA on-off switches affect cells ability to repair breaks, respond to chemotherapy
On the other hand, if acetylation is reduced, 53BP1 outcompetes BRCA1 at a break and the non-homologous end-joining tool repairs the break. This mechanism can help explain resistance to a promising chemotherapy called PARP inhibition seen in patients and mouse models with BRCA1 mutations. Work from several other research teams surprisingly has shown that if neither BRCA nor 53BP1 are available, then the homologous recombination system goes into action even in the absence of BRCA1 and BRCA1 mutant cancer cells become resistant to PARP inhibitors. Because of this, Greenberg says, there are some possible applications for making PARP chemotherapy more sensitive: If you could inhibit specific acetylation events, then a patients response to PARP inhibitors might be enhanced by hyperactivating 53BP1 binding to breaks in the context of BRCA1 deficient cancers. Whats more, measuring the levels of acetylation at H4 might predict how responsive an individual is to PARP inhibitors. The story didnt ...
http://www.innovations-report.com/html/reports/life-sciences/dna-switches-affect-cells-039-ability-repair-breaks-209016.html
Sir3 N-terminal acetylation stabilizes the interaction | Open-iSir3 N-terminal acetylation stabilizes the interaction | Open-i
Sir3 N-terminal acetylation stabilizes the interaction of Sir3 BAH with the NCP(a) Superposition of the structures of the N-terminally acetylated (pink) and una
https://openi.nlm.nih.gov/detailedresult.php?img=PMC3818696_emss-53785-f0002&req=4
Histone Deacetylases and Cancer - PubMedHistone Deacetylases and Cancer - PubMed
Reversible acetylation of histone and non-histone proteins is one of the most abundant post-translational modifications in eukaryotic cells. Protein acetylation and deacetylation are achieved by the antagonistic actions of two families of enzymes, histone acetyltransferases (HATs) and histone deacet …
https://pubmed.ncbi.nlm.nih.gov/22963873/
The Hark Lab Research Page | Muhlenberg CollegeThe Hark Lab Research Page | Muhlenberg College
Statement of Research Interests. My research interests focus on the regulation of gene transcription in eukaryotic organisms, and the consequences of this regulation for downstream developmental events. In particular, I am interested in how factors such as covalent modifications of histones, chromatin structure/architecture, and gene organization may act and interact to influence gene expression.. Impact of histone acetylation on plant development. Background on HATs and Histone Acetylation. One area of current research investigates the biological role of the histone acetyltransferase (HAT) enzyme GCN5 in developmental pathways in the model plant Arabidopsis thaliana. GCN5 can covalently modify histones (chromatin proteins) by catalyzing the addition of acetyl group to specific lysine residues. This modification is hypothesized to affect histone-DNA contacts or provide binding sites for other factors involved in regulating transcription (the histone code hypothesis), but the exact biochemical ...
https://www.muhlenberg.edu/main/academics/biology/facultystaff/amyhark/drharkspage/theharklabresearchpage/
Plus itPlus it
We have also investigated the potential roles of several signaling pathways, which could mediate apoptosis in the NPC cells upon treatment by bortezomib/SAHA. Bortezomib was found to potentiate SAHAs acetylation of histones H3 and H4 in the NPC cells. Furthermore, the histone acetylation was ROS- and caspase-8-dependent as both NAC- and caspase-8-specific inhibitor, Z-IETD-FMK, could markedly reduce the acetylation of the histones. The results were similar to the induction of caspase-8-dependent histone acetylation by combination of HDAC and proteasome inhibitors in leukemic cells (21). One of the major effects mediated by histone hyperacetylation is upregulation of tumor suppressor genes (9). However, we did not find any upregulation of retinoblastoma (Rb) or p53 in the bortezomib/SAHA-treated NPC cells (refer to Supplementary Fig. S2A). The p53 expression was repressed by the combination treatment in HA cells, whereas such repression was not found in C666-1 cells. Because enhanced apoptosis ...
http://mct.aacrjournals.org/content/12/5/747
Post-translational modificationsPost-translational modifications
Figure 3. Genetically encoded N-epsilon lysine acetylation allows the high resolution X-ray structures of acetylated proteins and their complexes to be solved. The high resolution structure of acetyl lysine from acetylated cyclophilin (left) showing the experimental density. Right, Acetylated cyclophilin in complex with cyclosporine. Water molecules (blue spheres) that are ordered at the protein small molecule interface in the unacetylated complex) rearrange in the acetylated complex.. The acetylation of a cyclophilin at Lys125 was identified in a proteomics screen. We subsequently demonstrated that a substantial proportion of CYPA is acetylated in HeLa cells and human T cell lines, suggesting that the acetylated form of the protein is biologically relevant. To test this, recombinant CYPA bearing homogenous acetylation at Lys125 was prepared by overexpression in E. coli using an acetyllysyl-tRNA synthetase-tRNACUA pair, allowing detailed biophysical and enzymatic characterization of acetylated ...
http://www2.mrc-lmb.cam.ac.uk/ccsb/?page_id=46
Acetylation-dependent regulation of MDM2 E3 ligase activity dictates its oncogenic function | Science SignalingAcetylation-dependent regulation of MDM2 E3 ligase activity dictates its oncogenic function | Science Signaling
p53 is the most well-characterized tumor suppressor, and its tumor-suppressive functions are dysregulated in more than 50% of human tumor tissues (29). The MDM2 oncogene is frequently amplified in many tumor tissues to functionally inactivate p53 (5), suggesting that inhibition of MDM2 activity might provide a robust method for cancer prevention. Previous studies have demonstrated that p300 cooperates with MDM2 and triggers p53 polyubiquitination as a ubiquitin E4 ligase (11, 30). Here, we propose a novel regulatory mechanism for p300 via acetylation-dependent control of the oncogenic function of MDM2. Specifically, p300 directly acetylates MDM2, a process that channels that E3 ligase activity away from MDM2 autoubiquitination to concentrate on promoting p53 ubiquitination, in part because of acetylation-induced alteration of intramolecular interaction of MDM2. Thus, p300 may regulate p53 ubiquitination through multiple regulatory mechanisms.. Furthermore, we identified that acetylation of MDM2 ...
https://stke.sciencemag.org/content/10/466/eaai8026
Duration of Nuclear NF-κB Action Regulated by Reversible Acetylation | ScienceDuration of Nuclear NF-κB Action Regulated by Reversible Acetylation | Science
Studies were next performed to define the molecular basis for HDAC3-mediated inhibition of TNF-α activation of NF-κB. Expression of HDAC3 in HeLa cells (∼50% transfection efficiency) diminished both NF-κB DNA-binding activity (Fig. 3C, panel 1) and levels of nuclear RelA (panel 4). In contrast, HDAC3 expression did not alter TNF-α-induced degradation of IκBα occurring in the cytoplasm (panel 3), nor did it markedly change the levels of Sp1 DNA-binding activity (panel 2). Together with the results presented in Fig. 1C, these findings raise the possibility that HDAC3 may regulate the intracellular trafficking of RelA. A precedent for acetylation influencing the intracellular trafficking of a transcription factor is provided by recent studies on hepatocyte nuclear factor-4 and class II transactivator (CIITA) (21, 22). To monitor potential effects of HDAC3 on the intracellular trafficking of RelA, we expressed green fluorescent protein-RelA fusion proteins (GFP-RelA) in HeLa cells in the ...
https://science.sciencemag.org/content/293/5535/1653?ijkey=40713be53e1459fb5da9b7d99b6a6e9113c82e1b&keytype2=tf_ipsecsha
AID 632979 - Inhibition of HDAC4 in human HL60 cells assessed as increase in histone H4 acetylation at 1 ug/ml after 24 hrs by...AID 632979 - Inhibition of HDAC4 in human HL60 cells assessed as increase in histone H4 acetylation at 1 ug/ml after 24 hrs by...
BioAssay record AID 632979 submitted by ChEMBL: Inhibition of HDAC4 in human HL60 cells assessed as increase in histone H4 acetylation at 1 ug/ml after 24 hrs by Western blot analysis.
https://pubchem.ncbi.nlm.nih.gov/bioassay/632979
Specific Inhibition of p300-HAT Alters Global Gene Expression and Represses HIV Replication ePrints@IIScSpecific Inhibition of p300-HAT Alters Global Gene Expression and Represses HIV Replication [email protected]
Reversible acetylation of histone and nonhistone proteins plays pivotal role in cellular homeostasis. Dysfunction of histone acetyltransferases (HATs) leads to several diseases including cancer, neurodegenaration, asthma, diabetes, AIDS, and cardiac hypertrophy. We describe the synthesis and characterization of a set of p300-HAT-specific small-molecule inhibitors from a natural nonspecific HAT inhibitor, garcinol, which is highly toxic to cells. We show that the specific inhibitor selectively represses the p300-mediated acetylation of p53 in vivo. Furthermore, inhibition of p300-HAT down regulates several genes but significantly a few important genes are also upregulated. Remarkably, these inhibitors were found to be nontoxic to T cells, inhibit histone acetylation of HIV infected cells, and consequently inhibit the multiplication of HIV.. ...
http://eprints.iisc.ac.in/11918/
Aspirin chemically modifies human hemoglobin through acetylation.Aspirin chemically modifies human hemoglobin through acetylation.
Greenmedinfo.com - Natural Health Resource - The worlds most widely referenced, open access, natural medicine database, with 30,000+ study abstracts and growing daily
http://www.greenmedinfo.com/article/aspirin-chemically-modifies-human-hemoglobin-through-acetylation
A Genetic Screen for Ribosomal DNA Silencing Defects Identifies Multiple DNA Replication and Chromatin-Modulating Factors |...A Genetic Screen for Ribosomal DNA Silencing Defects Identifies Multiple DNA Replication and Chromatin-Modulating Factors |...
Histone deacetylation and rDNA silencing.Transcriptionally silenced regions of eukaryotic genomes are generally hypoacetylated. In S. cerevisiae, the HM loci and telomeres are hypoacetylated on histones H3 and H4, with an acetylation pattern similar to that in higher eukaryotes (10). The yeast RPD3 gene product is the proposed catalytic component of a large multiprotein histone deacetylase complex (HDB) which acts in targeted transcriptional repression (40, 64). Sin3p, another subunit of HDB, acts to target the complex to specific Pol II promoters through association with specific DNA binding proteins (41). Sap30p is also a member of this complex (84). Paradoxically,rpd3 and sin3 mutations increase silencing atHM loci (77), telomeres (23, 64), and the rDNA (this study). Similarly, Drosophila TPE is enhanced by null mutations of its RPD3 homolog (23). In another study, sap30 mutants strengthened all types of silencing in yeast, including the rDNA (37). These findings were completely consistent ...
https://mcb.asm.org/content/19/4/3184?ijkey=17d1e833fd3d76ee6496ee1a7b695c4a15ed4f0a&keytype2=tf_ipsecsha
Research Grants - 2009Research Grants - 2009
Histones are proteins in close contact with the DNA in the nucleus of a cell. They function to maintain the organization of DNA, and recent studies have shown that histones regulate the expression of genes. Histones can be modified by biochemical processes such as addition or removal of acetyl groups, known as acetylation and deacetylation. Such modifications have been shown to control genetic mechanisms important for memory storage in brain cells.. Ottavio Arancio, M.D. and colleagues studied mice that had been genetically altered to exhibit Alzheimer-like pathology. They found that long-term potentiation-a cellular model of memory formation in the brain-was reduced by drugs that prevent deacetylation of histones. Furthermore, acetylation of histones in these animals was greatly reduced after a behavioral training session in comparison to normal mice. They have proposed to extend these studies to examine whether impairments in histone acetylation or deacetylation are involved in the memory ...
https://www.actionalz.org/research/alzheimers_grants/for_researchers/overview-2009.asp?grants=2009arancio
Protein acetylation as a means to regulate protein function in tune with metabolic state - Fingerprint - University of...
Fingerprint Dive into the research topics of Protein acetylation as a means to regulate protein function in tune with metabolic state. Together they form a unique fingerprint. ...
https://utsouthwestern.pure.elsevier.com/en/publications/protein-acetylation-as-a-means-to-regulate-protein-function-in-tu/fingerprints/
Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns - Zurich Open...Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns - Zurich Open...
Lysine acetylation is a major posttranslational modification involved in a broad array of physiological functions. Here, we provide an organ-wide map of lysine acetylation sites from 16 rat tissues analyzed by high-resolution tandem mass spectrometry. We quantify 15,474 modification sites on 4,541 proteins and provide the data set as a web-based database. We demonstrate that lysine acetylation displays site-specific sequence motifs that diverge between cellular compartments, with a significant fraction of nuclear sites conforming to the consensus motifs G-AcK and AcK-P. Our data set reveals that the subcellular acetylation distribution is tissue-type dependent and that acetylation targets tissue-specific pathways involved in fundamental physiological processes. We compare lysine acetylation patterns for rat as well as human skeletal muscle biopsies and demonstrate its general involvement in muscle contraction. Furthermore, we illustrate that acetylation of fructose-bisphosphate aldolase and ...
http://www.zora.uzh.ch/id/eprint/66903/
The actin-MRTF-SRF transcriptional circuit controls tubulin acetylation via α-TAT1 gene expression | JCBThe actin-MRTF-SRF transcriptional circuit controls tubulin acetylation via α-TAT1 gene expression | JCB
The role of formins in microtubules is not well understood. In this study, we have investigated the mechanism by which INF2, a formin mutated in degenerative renal and neurological hereditary disorders, controls microtubule acetylation. We found that silencing of INF2 in epithelial RPE-1 cells produced a dramatic drop in tubulin acetylation, increased the G-actin/F-actin ratio, and impaired myocardin-related transcription factor (MRTF)/serum response factor (SRF)-dependent transcription, which is known to be repressed by increased levels of G-actin. The effect on tubulin acetylation was caused by the almost complete absence of α-tubulin acetyltransferase 1 (α-TAT1) messenger RNA (mRNA). Activation of the MRTF-SRF transcriptional complex restored α-TAT1 mRNA levels and tubulin acetylation. Several functional MRTF-SRF-responsive elements were consistently identified in the α-TAT1 gene. The effect of INF2 silencing on microtubule acetylation was also observed in epithelial ECV304 cells, but not ...
http://jcb.rupress.org/content/early/2018/01/09/jcb.201702157
Changed Histone Acetylation Patterns in Normal-Appearing White Matter and Early Multiple Sclerosis Lesions | Journal of...Changed Histone Acetylation Patterns in Normal-Appearing White Matter and Early Multiple Sclerosis Lesions | Journal of...
In this study, we analyzed the equilibrium between histone acetylation, mediated by HATs, and histone deacetylation, mediated by HDACs, in the NAWM of chronic MS brains. Similar to what was reported for the old rodent brain, also in the NAWM of human brains from aged individuals and chronic MS patients we detected a shift toward acetylation. This shift toward acetylation detected in a subset of female patients correlated with the consistent and reproducible increase of several histone acetyltransferase family members, including CBP, P300, MYST3, and MYST4. It is worth noting that, although we also detected increased levels of HDAC11 in this subpopulation, the increased of the acetyltransferases was much greater and likely determined the shift in favor of increased acetylation. These differences were most prominent in a subset of female MS patients and were associated with high levels of developmentally regulated genes (i.e., TCF7L2, SOX2, ID2) compared with controls. Several other genes (i.e., ...
http://www.jneurosci.org/content/31/9/3435.long
Acetylation Phenotype in Colorectal Carcinoma | Cancer ResearchAcetylation Phenotype in Colorectal Carcinoma | Cancer Research
Sulfamethazine acetylation phenotype was determined in 49 patients with cancer of the colon or rectum, 41 old, and 45 young control subjects. Metabolic clearance of sulfamethazine, plasma ratio of N-acetylsulfamethazine:N-acetylsulfamethazine plus sulfamethazine and urinary ratio of N-acetylsulfamethazine:N-acetylsulfamethazine plus sulfamethazine were used to classify subjects into slow and fast acetylation phenotypes. All three measures gave similar results. The proportions of slow and fast acetylators were similar in both control groups and there were significantly more fast acetylators in the cancer group than in the control groups (ϰ2 = 5.0-8.5; P , 0.05). The data suggest that there may be an association between acetylation phenotype and colorectal carcinoma.. ...
https://cancerres.aacrjournals.org/content/47/5/1466?ijkey=fac728fb176abc5df278e11e2dfd18d6c02036b4&keytype2=tf_ipsecsha
Structural Biochemistry/Enzyme Regulation/Protein Lysine Acetylation in metabolism - Wikibooks, open books for an open worldStructural Biochemistry/Enzyme Regulation/Protein Lysine Acetylation in metabolism - Wikibooks, open books for an open world
Enoyl-coenzyme A hydratase/3-hydroxyacyl-coenzyme A (EHHADH) is an important enzyme which catalyze two steps in fatty acid oxidation. There are four acetylated lysine residues been identified in EHHADH, which are Lys165, Lys171, Lys346 and Lys584. Immunoprecipitation of ectopically expressed FLAG-tagged EHHADH and Western blotting with antibody to acetyllysine confirmed that EHHADH was indeed acetylated(Zhao, et al). In order to explore the effect of acetylation on fatty acid oxidation. Isobaric tags are used, which is TSA and NAM. TSA and NAM treatment increased all the four lysine residues acetylation. Consistently, corresponding unacetylated peptide was decreased. Scientists treat TSA and NAM to Chang Human Liver cells doubled the activity of EHHADH, which indicates that acetylation of EHHADH would increase fatty acid oxidation pathway. In order to confirm the result, site-directed mutagenesis was used and the four lysine residue was replaced by glutamine, TSA and NAM can no longer ...
https://en.m.wikibooks.org/wiki/Structural_Biochemistry/Enzyme_Regulation/Protein_Lysine_Acetylation_in_metabolism
Restricted mitochondrial protein acetylation initiates mitochondrial autophagy | Journal of Cell ScienceRestricted mitochondrial protein acetylation initiates mitochondrial autophagy | Journal of Cell Science
Because increased mitophagic flux enhances yeast mitochondrial fidelity (Kurihara et al., 2012) and more efficient mitochondrial function might ameliorate redox stress injury, we explored whether GCN5L1 KD cells are stress resilient. GCN5L1 depletion decreased rotenone-induced reactive oxygen species (ROS) generation, susceptibility to ionomycin-induced mitochondrial permeability transition, and enhanced resistance to paraquat-induced cell death (Fig. 4D-F).. To begin exploring this biology in vivo, GCN5L1-knockout (KO) mice were generated, but these were not viable. However, KO MEFs were harvested and showed the same mitochondrially restricted reduction in protein acetylation (supplementary material Fig. S5A,B) and mitochondrial enrichment of autophagy mediators (Fig. 4G; supplementary material Fig. S5C) as found in the KD studies. In addition, the reconstitution of GCN5L1 in these KO MEFs restored mitochondrial acetylation and reversed the mitochondrial accumulation of p62 and LC3-II (Fig. 4H; ...
http://jcs.biologists.org/content/126/21/4843
Snf1 - A histone kinase that works in concert with the histone acetyltransferase Gcn5 to regulate transcription<...
TY - JOUR. T1 - Snf1 - A histone kinase that works in concert with the histone acetyltransferase Gcn5 to regulate transcription. AU - Lo, W. S.. AU - Duggan, L.. AU - Emre, N. C.T.. AU - Belotserkovskya, R.. AU - Lane, W. S.. AU - Shiekhattar, R.. AU - Berger, S. L.. PY - 2001/8/10. Y1 - 2001/8/10. N2 - Modification of histones is an important element in the regulation of gene expression. Previous work suggested a link between acetylation and phosphorylation, but questioned its mechanistic basis. We have purified a histone H3 serine-10 kinase complex from Saccharomyces cerevisiae and have identified its catalytic subunit as Snf1. The Snf1/AMPK family of kinases function in conserved signal transduction pathways. Our results show that Snf1 and the acetyltransferase Gcn5 function in an obligate sequence to enhance INO1 transcription by modifying histone H3 serine-10 and lysine-14. Thus, phosphorylation and acetylation are targeted to the same histone by promoter-specific regulation by a ...
https://miami.pure.elsevier.com/en/publications/snf1-a-histone-kinase-that-works-in-concert-with-the-histone-acet
βPix-d promotes tubulin acetylation and neurite outgrowth through a PAK/Stathmin1 signaling pathway<...
TY - JOUR. T1 - βPix-d promotes tubulin acetylation and neurite outgrowth through a PAK/Stathmin1 signaling pathway. AU - Kwon, Younghee. AU - Jeon, Ye Won. AU - Kwon, Minjae. AU - Cho, Yongcheol. AU - Park, Dongeun. AU - Shin, Jung Eun. PY - 2020. Y1 - 2020. N2 - Microtubules are a major cytoskeletal component of neurites, and the regulation of microtubule stability is essential for neurite morphogenesis. βPix (ARHGEF7) is a guanine nucleotide exchange factor for the small GTPases Rac1 and Cdc42, which modulate the organization of actin filaments and microtubules. βPix is expressed as alternatively spliced variants, including the ubiquitous isoform βPix-a and the neuronal isoforms βPix-b and βPix-d, but the function of the neuronal isoforms remains unclear. Here, we reveal the novel role of βPix neuronal isoforms in regulating tubulin acetylation and neurite outgrowth. At DIV4, hippocampal neurons cultured from βPix neuronal isoform knockout (βPix-NIKO) mice exhibit defects in neurite ...
https://koreauniv.pure.elsevier.com/en/publications/%CE%B2pix-d-promotes-tubulin-acetylation-and-neurite-outgrowth-through
The immunoglobulin heavy chain locus control region increases histone acetylation along linked c-myc genes - WRAP: Warwick...The immunoglobulin heavy chain locus control region increases histone acetylation along linked c-myc genes - WRAP: Warwick...
In chromosome translocations characteristic of Burkitt lymphomas (BL) and murine plasmacytomas, c-myc genes become juxtaposed to immunoglobulin heavy-chain (IgH) sequences, resulting in aberrant c-myc transcription. Translocated c-myc alleles that retain the first exon exhibit increased transcription from the normally minor c-myc promoter, P1, and increased transcriptional elongation through inherent pause sites proximal to the major c-myc promoter, P2, We recently demonstrated that a cassette derived from four DNase I-hypersensitive sites (HS1234) in the 3C alpha region of the IgH locus functions as an enhancer-locus control region (LCR) and directs a similar pattern of deregulated expression of linked c-myc genes in BL and plasmacytoma cell lines. Here, we report that the HS1234 enhancer-LCR mediates a widespread increase in histone acetylation along linked c-myc genes in Raji BL cells. Significantly, the increase in acetylation was not restricted to nucleosomes within the promoter region but ...
http://wrap.warwick.ac.uk/15276/
Histone Acetylation at Promoters Is Differentially Affected by Specific Activators and Repressors | Molecular and Cellular...Histone Acetylation at Promoters Is Differentially Affected by Specific Activators and Repressors | Molecular and Cellular...
Transcription in eukaryotes occurs in the context of DNA packaged into chromatin. The basic unit of chromatin is the nucleosome, in which DNA is wrapped around the core histones H2A, H2B, H3, and H4. Nucleosome remodeling complexes such as Swi-Snf can facilitate opening of repressive chromatin structures in promoter regions to provide access for DNA-binding activator proteins or general transcription factors (32). In addition, reversible chromatin modifications such as acetylation, phosphorylation, and methylation of N-terminal histone tails can modulate accessibility of DNA within chromatin (56). Acetylation of lysines in the histone tails neutralizes their positive charge, thereby weakening electrostatic interactions with DNA (25) and interactions between neighboring nucleosomes (42). The tails of histones H3 and H4 are important for transcriptional regulation of numerous genes, because mutations in these histone tails result in both derepression and diminished activation (15, 44). ...
https://mcb.asm.org/content/21/8/2726?ijkey=ef09a91a1d906e523211259ad8d94df2286db5b9&keytype2=tf_ipsecsha
Creating a pro-survival and anti-inflammatory phenotype by modulation of acetylation in models of hemorrhagic and septic shock<...
TY - CHAP. T1 - Creating a pro-survival and anti-inflammatory phenotype by modulation of acetylation in models of hemorrhagic and septic shock. AU - Li, Yongqing. AU - Alam, Hasan B.. N1 - Copyright: Copyright 2012 Elsevier B.V., All rights reserved.. PY - 2012. Y1 - 2012. N2 - Shock, regardless of etiology, is characterized by decreased tissue perfusion resulting in cell death, organ dysfunction, and poor survival. Current therapies largely focus on restoring tissue perfusion through resuscitation but have failed to address the specific cellular dysfunction caused by shock. Acetylation is rapidly emerging as a key mechanism that regulates the expression of numerous genes (epigenetic modulation through activation of nuclear histone proteins), as well as functions of multiple cytoplasmic proteins involved in key cellular functions such as cell survival, repair/healing, signaling, and proliferation. Cellular acetylation can be increased immediately through the administration of histone deacetylase ...
https://www.scholars.northwestern.edu/en/publications/creating-a-pro-survival-and-anti-inflammatory-phenotype-by-modula
Analysis of the histone deacetylases disruptants of Aspergillus oryzae | Aspergillus & Aspergillosis WebsiteAnalysis of the histone deacetylases disruptants of Aspergillus oryzae | Aspergillus & Aspergillosis Website
Aspergillus oryzae is ubiquitous filamentous fungi in nature and has been used in a number of industries such as Japanese traditional fermented foods and pharmaceutical products. In nature and industries, A. oryzae adapt to various environmental conditions by global change of transcriptional regulation. In the previous study, we revealed that the expression of histone acetylation related genes were affected by the growth phase and alteration of growing environment, such as culture conditions and stress exposing. In general, histone acetylation plays the fundamental roles for the genes expression, and closely relates to growth, morphology, differentiation and stress responses. Recently, several reports indicate that histone acetylation also plays important roles in filamentous fungi. In this context, we focused on histone acetylation related genes, particularly histone deacetylases (HDACs). We attempted to disrupt 11 HDACs homologue of A. oryzae and 10 of them were disrupted. However, in the ...
https://www.aspergillus.org.uk/content/analysis-histone-deacetylases-disruptants-aspergillus-oryzae
Proteome-wide Analysis of Lysine Acetylation Suggests its Broad Regulatory Scope in Saccharomyces cerevisiae :: MPG.PuReProteome-wide Analysis of Lysine Acetylation Suggests its Broad Regulatory Scope in Saccharomyces cerevisiae :: MPG.PuRe
Author: Henriksen, Peter et al.; Genre: Journal Article; Published in Print: 2012-11; Open Access; Keywords: SISTER-CHROMATID COHESION; MASS-SPECTROMETRY; HISTONE ACETYLATION;|br/| SIGNALING NETWORKS; ESCHERICHIA-COLI; C-TRAP; YEAST; COMPLEXES;|br/| DEACETYLATION; PHOSPHORYLATION; Title: Proteome-wide Analysis of Lysine Acetylation Suggests its Broad|br/| Regulatory Scope in Saccharomyces cerevisiae
http://pubman.mpdl.mpg.de/pubman/faces/viewItemOverviewPage.jsp?itemId=escidoc:1651227
Signal-Seeker™ Phosphorylation SUMOylation 2/3 Acetylation and Ubiquitination Enrichment Kits - Cytoskeleton, Inc.Signal-Seeker™ Phosphorylation SUMOylation 2/3 Acetylation and Ubiquitination Enrichment Kits - Cytoskeleton, Inc.
The Signal-Seeker kits detect total and protein specific protein modifications of ubiquitination, ubiquitylation, sumoylation, sumoylation 2/3, acetylation, and tyrosine phosphorylation using the following kits and reagents: Phosphotyrosine kit, Phosphorylation kit, Tyrosine Phosphorylation kit, Ubiquitin kit, Ubiquitination kit, SUMOylation kit, SUMOylation 2/3 kit, SUMO kit, SUMO 2/3 kit, Phosphotyrosine affinity kit, Phosphorylation affinity kit, Tyrosine affinity Phosphorylation kit, Ubiquitin affinity kit, Ubiquitination affinity kit, SUMOylation affinity kit, SUMOylation affinity 2/3 kit, SUMO affinity kit, SUMO affinity 2/3 kit, Phosphotyrosine enrichment kit, Phosphorylation enrichment kit, Tyrosine Phosphorylation enrichment kit, Ubiquitin enrichment kit, Ubiquitination enrichment kit, SUMOylation enrichment kit, SUMOylation enrichment 2/3 kit, SUMO enrichment kit, SUMO enrichment 2/3 kit, acetylation affinity kit, acetyl-lysine affinity kit, acetyl-lysine kit, acetylation kit, acetyl-lysine
https://www.cytoskeleton.com/signal-seeker
Histone acetylation in vivo at the osteocalcin locus is functionally l by Jiali Shen, Martin A. Montecino et al.Histone acetylation in vivo at the osteocalcin locus is functionally l by Jiali Shen, Martin A. Montecino et al.
The accessibility of regulatory elements in chromatin represents a principal rate-limiting parameter of gene transcription and is modulated by enzymatic transcriptional co-factors that alter the topology of chromatin or covalently modify histones (e.g. by acetylation). The bone-specific activation and 1,25-dihydroxyvitamin D(3) enhancement of osteocalcin (OC) gene transcription are both functionally linked to modifications in nucleosomal organization. The initiation of tissue-specific basal transcription is accompanied by the induction of two DNase I hypersensitive sites, and this chromatin remodeling event requires binding of the key osteogenic factor RUNX2/CBFA1 to the OC promoter. Here, we analyzed the acetylation status of histones H3 and H4 when the OC gene is active (in osteoblastic ROS17/2.8 cells) or inactive (in fibroblastic ROS24/1 cells) using chromatin immunoprecipitation assays. We find that acetylated histone H3 and H4 proteins are associated with the OC promoter only when the gene is
https://escholarship.umassmed.edu/gsbs_sp/1107/
Rapid and transient oxygen consumption increase following acute HDAC/KDAC inhibition in Drosophila tissue | Scientific ReportsRapid and transient oxygen consumption increase following acute HDAC/KDAC inhibition in Drosophila tissue | Scientific Reports
Epigenetic deregulation, such as the reduction of histone acetylation levels, is thought to be causally linked to various maladies associated with aging. Consequently, histone deacetylase inhibitors are suggested to serve as epigenetic therapy by increasing histone acetylation. However, previous work suggests that many non-histone proteins, including metabolic enzymes, are also acetylated and that post transitional modifications may impact their activity. Furthermore, deacetylase inhibitors were recently shown to impact the acetylation of a variety of proteins. By utilizing a novel technique to measure oxygen consumption rate from whole living tissue, we demonstrate that treatment of whole living fly heads by the HDAC/KDAC inhibitors sodium butyrate and Trichostatin A, induces a rapid and transient increase of oxygen consumption rate. In addition, our study indicates that the rate increase is markedly attenuated in midlife fly head tissue. Overall, our data suggest that HDAC/KDAC inhibitors may induce
https://www.nature.com/articles/s41598-018-22674-2/?error=cookies_not_supported&code=f0616909-c033-48dc-9f7d-f85aed81a1b5
2rny - Proteopedia, life in 3D2rny - Proteopedia, life in 3D
Histone lysine acetylation is central to epigenetic control of gene transcription. Bromodomains of chromosomal proteins function as acetyl-lysine (Kac) binding domains. However, how bromodomains recognize site-specific histones remains unanswered. Here, we report three three-dimensional solution structures of the bromodomains of the human transcriptional coactivators CREB-binding protein (CBP) and p300/CBP-associated factor (PCAF) bound to peptides derived from histone acetylation sites at lysines 36 and 9 in H3, and lysine 20 in H4. From structural and biochemical binding analyses, we determine consensus histone recognition by the bromodomains of PCAF and CBP, which represent two different subgroups of the bromodomain family. Through bromodomain residues in the ZA and BC loops, PCAF prefers acetylation sites with a hydrophobic residue at (Kac+2) position and a positively charged or aromatic residue at (Kac+3), whereas CBP favors bulky hydrophobic residues at (Kac+1) and (Kac+2), a positively ...
http://proteopedia.org/wiki/index.php/2rny
Global assessment of combinatorial post-translational modification of core histones in yeast using contemporary mass...Global assessment of combinatorial post-translational modification of core histones in yeast using contemporary mass...
A global view of all core histones in yeast is provided by tandem mass spectrometry of intact histones H2A, H2B, H4, and H3. This allowed detailed characterization of ,50 distinct histone forms and their semiquantitative assessment in the deletion mutants gcn5Delta, spt7Delta, ahc1Delta, and rtg2Delta, affecting the chromatin remodeling complexes SAGA, SLIK, and ADA. The top down mass spectrometry approach detected dramatic decreases in acetylation on H3 and H2B in gcn5Delta cells versus wild type. For H3 in wild type cells, tandem mass spectrometry revealed a direct correlation between increases of Lys(4) trimethylation and the 0, 1, 2, and 3 acetylation states of histone H3. The results show a wide swing from 10 to 80% Lys(4) trimethylation levels on those H3 tails harboring 0 or 3 acetylations, respectively. Reciprocity between these chromatin marks was apparent, since gcn5Delta cells showed a 30% decrease in trimethylation levels on Lys(4) in addition to a decrease of acetylation levels on ...
https://www.scienceexchange.com/publications/6785
TFBSbank: a dedicated and comprehensive platform to dissect the big data of protein-DNA interaction in model species
GO Terms Descrition:, locomotor rhythm, positive regulation of transcription from RNA polymerase II promoter, R3/R4 cell fate commitment, synapse assembly, compound eye development, hemopoiesis, transcription factor binding, compound eye morphogenesis, protein complex, protein binding, smoothened signaling pathway, neurogenesis, Wnt signaling pathway, glial cell migration, zinc ion binding, nucleus, neurotransmitter secretion, transcription cofactor activity, histone acetyltransferase activity, regulation of transcription, DNA-templated, histone H4-K12 acetylation, histone H4-K8 acetylation, histone H3-K27 acetylation, histone H3-K18 acetylation, histone acetyltransferase activity (H3-K18 specific), histone acetyltransferase activity (H3-K27 specific), cAMP response element binding protein binding, circadian regulation of gene expression, DNA replication checkpoint, R7 cell differentiation, transcription coactivator activity, regulation of mitosis ...
http://tfbsbank.co.uk/result.php?jobdir=Results/DB0022/
H3K27ac acetylome signatures reveal the epigenomic reorganization in remodeled non-failing human hearts - MDR ResearchH3K27ac acetylome signatures reveal the epigenomic reorganization in remodeled non-failing human hearts - MDR Research
We detected chromatin regions with differential acetylation activity (DARs; Padj. , 0.05) between remodeled non-failing patient hearts and healthy donor hearts. The acetylation level of the chromatin region correlated with its RNA polymerase II occupancy level and the mRNA expression level of its adjacent gene per sample. Annotated genes from DARs were enriched in disease-related pathways, including fibrosis and cell metabolism regulation. DARs that change in the same direction have a tendency to cluster together, suggesting the well-reorganized chromatin architecture that facilitates the interactions of regulatory domains in response to myocardial remodeling. We further show the differences between the acetylation level and the mRNA expression level of cell-type-specific markers for cardiomyocytes and 11 non-myocyte cell types. Notably, we identified transcriptome factor (TF) binding motifs that were enriched in DARs and defined TFs that were predicted to bind to these motifs. We further showed ...
https://mdrresearch.nl/publication/h3k27ac-acetylome-signatures-reveal-the-epigenomic-reorganization-in-remodeled-non-failing-human-hearts/
Epigenetic hypothesis tests for methylation and acetylation in a triple microarray system<...
TY - JOUR. T1 - Epigenetic hypothesis tests for methylation and acetylation in a triple microarray system. AU - Li, Lang. AU - Shi, Huidong. AU - Yiannoutsos, Constantin. AU - Huang, Tim Hui Ming. AU - Nephew, Kenneth P.. PY - 2005. Y1 - 2005. N2 - To fully elucidate the functional relationship between DNA methylation and histone hypoacetylation in gene silencing, we have developed an integrated triple microarray system that allows us to begin to decipher the influence of epigenetic hierarchies on the regulation of gene expression in cancer cells. Our hypothesis is that in the promoter region of a silenced gene, reversal of two epigenetic factors (i.e., DNA demethylation and/or histone hyperacetylation) is highly correlated with gene reexpression after treatment of the human epithelial ovarian cancer cell line CP70 with the drug combination 5-aza-2′-deoxycytidine (DAC), a demethylating agent, and trichostatin A (TSA), an inhibitor of histone deacetylases. To estimate the posterior ...
https://augusta.pure.elsevier.com/en/publications/epigenetic-hypothesis-tests-for-methylation-and-acetylation-in-a-
BioProduct: Immunology: Immunocytochemistry Reagents: Histone H3 Phospho (pS10) DyLight 488: Histone H3 Phospho (pS10) DyLight...BioProduct: Immunology: Immunocytochemistry Reagents: Histone H3 Phospho (pS10) DyLight 488: Histone H3 Phospho (pS10) DyLight...
Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (5 ...
http://www.protocol-online.org/bioproduct/Product_listing/Histone-H3-Phospho--pS10--DyLight-488-509.html
Epigenetic Regulation of BDNF Gene in Response to StressEpigenetic Regulation of BDNF Gene in Response to Stress
Several studies have recently reported that chromatin-modifying mechanisms are involved in learning and memory processes in adult neurons.3,5,35 For example, Levenson et al.35 showed that whereas histone H3 was acetylated following contextual FC in the hippocampus, histone H4 was acetylated following a latent inhibition protocol for contextual FC. Lubin et al.3 investigated histone modifications at specific BDNF promoters after contextual FC. They observed a selective increase in histone H3 acetylation at the BDNF gene promoter of exon IV, and a selective decrease in histone H4 acetylation at the BDNF promoter of exon II. In our study, SPS rats demonstrated a significant increase relative to sham rats in the levels of acetylated H3 and H4 at BDNF promoters of exons I and IV after FC. In addition, this selective increase in histone acetylation at the promoters of exons I and IV were consistent with up-regulation in exon I and IV mRNA transcription associated with FC. Interestingly, there were no ...
http://psychiatryinvestigation.org/journal/view.php?number=538
北京大学医学部机构知识库([email protected]): Role of histone deacetylation in cell-specific expression of endothelial nitric-oxide synthase
Histone acetylation plays an important role in chromatin remodeling and gene expression. The molecular mechanisms involved in cell-specific expression of endothelial nitric-oxide synthase ( eNOS) are not fully understood. In this study we investigated whether histone deacetylation was involved in repression of eNOS expression in non-endothelial cells. Induction of eNOS expression by histone deacetylase ( HDAC) inhibitors trichostatin A (TSA) and sodium butyrate was observed in all four different types of non-endothelial cells examined. Chromatin immunoprecipitation assays showed that the induction of eNOS expression by TSA was accompanied by a remarkable increase of acetylation of histone H3 associated with the eNOS 5′-flanking region in the non-endothelial cells. Moreover, DNA methylation-mediated repression of eNOS promoter activity was partially reversed by TSA treatment, and combined treatment of TSA and 5-aza-2′-deoxycytidine (AzadC) synergistically induced eNOS expression in ...
http://ir.bjmu.edu.cn/handle/400002259/59728
Selective N-terminal fluorescent labeling of proteins using 4-chloro-7-nitrobenzofurazan: A method to distinguish protein N...
TY - JOUR. T1 - Selective N-terminal fluorescent labeling of proteins using 4-chloro-7-nitrobenzofurazan. T2 - A method to distinguish protein N-terminal acetylation. AU - Bernal-Perez, Lina F.. AU - Prokai, Laszlo. AU - Ryu, Youngha. PY - 2012/9/1. Y1 - 2012/9/1. N2 - A fluorogenic derivatization method was developed to distinguish the protein N-terminal acetylation status. The unacetylated protein selectively reacted with 4-chloro-7-nitrobenzofurazan (NBD-Cl) at neutral pH to provide high fluorescence. In contrast, the protein with N-terminal acetylation was essentially nonfluorescent under the same conditions despite the presence of many internal lysine residues. Fluorescence of the NBD-labeled protein was very stable, and only micromolar concentrations of proteins were required for reliable detection. This method also provides a general and practical way to quantify proteins when their N-terminal amino group is available.. AB - A fluorogenic derivatization method was developed to distinguish ...
https://experts.unthsc.edu/en/publications/selective-n-terminal-fluorescent-labeling-of-proteins-using-4-chl
Liqiang Chen, PhD | College of Pharmacy - University of MinnesotaLiqiang Chen, PhD | College of Pharmacy - University of Minnesota
Research Interests. We have been interested in a family of enzymes named histone deacetylase (HDAC), which catalyzes the deacetylation of the acetyl lysine residue on histone tails. There are 18 members of human HDAC which can be divided into four classes. Class I, II and IV HDACs require zinc metal whereas Class III HDACs, which are named sirtuins, are NAD-dependent. Counteracting histone acetyltransferase (HAT), HDAC controls the histone acetylation level, structural modification of chromatin, and subsequent regulation of genes that are implicated in cell growth, proliferation, and differentiation. Furthermore, many non-histone proteins have been identified as HDAC substrates. Numerous studies have demonstrated that inhibitions of HDAC offer therapeutic benefits.. Research Projects. Applying the principles of fragment- and structure-based drug design, we are currently investigating novel structural templates for the discovery of sirtuin inhibitors. Our strategy is to design, synthesize and ...
https://www.pharmacy.umn.edu/bio/pharmacy-faculty-a-z/liqiang-chen
Replication-independent nucleosome exchange is enhanced by local and specific acetylation of histone H4
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
http://pubmedcentralcanada.ca/pmcc/articles/PMC3575802/?lang=en-ca
Displacement of Histones at Promoters of <i>Saccharomyces cerevisiae</ by Tamara Y. Erkina and Alexander...Displacement of Histones at Promoters of <i>Saccharomyces cerevisiae</ by Tamara Y. Erkina and Alexander...
Chromatin remodeling at promoters of activated genes spans from mild histone modifications to outright displacement of nucleosomes in trans. Factors affecting these events are not always clear. Our results indicate that histone H3 acetylation associated with histone displacement differs drastically even between promoters of such closely related heat shock genes as HSP12, SSA4, and HSP82. The HSP12 promoter, with the highest level of histone displacement, showed the highest level of H3 acetylation, while the SSA4 promoter, with a lower histone displacement, showed only modest H3 acetylation. Moreover, for the HSP12 promoter, the level of acetylated H3 is temporarily increased prior to nucleosome departure. Individual promoters in strains expressing truncated versions of heat shock factor (HSF) showed that deletion of either one of two activating regions in HSF led to the diminished histone displacement and correspondingly lower H3 acetylation. The deletion of both regions simultaneously severely
https://digitalcommons.butler.edu/cophs_papers/146/
Kat8 - Histone acetyltransferase KAT8 - Mus musculus (Mouse) - Kat8 gene & proteinKat8 - Histone acetyltransferase KAT8 - Mus musculus (Mouse) - Kat8 gene & protein
Histone acetyltransferase which may be involved in transcriptional activation. May influence the function of ATM. As part of the MSL complex it is involved in acetylation of nucleosomal histone H4 producing specifically H4K16ac. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. That activity is less specific than the one of the MSL complex. Can also acetylate TP53/p53 at Lys-120.
http://www.uniprot.org/uniprot/Q9D1P2
Acetylation phenotype and cytochrome P450IA2 phenotype are unlikely to be associated with peripheral arterial disease -...
Acetylation phenotype and cytochrome P450IA2 phenotype are unlikely to be associated with peripheral arterial disease Academic Article ...
https://experts.mcmaster.ca/display/publication1790442
Kat7 - Histone acetyltransferase KAT7 - Rattus norvegicus (Rat) - Kat7 gene & proteinKat7 - Histone acetyltransferase KAT7 - Rattus norvegicus (Rat) - Kat7 gene & protein
Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Involved in H3K14 (histone H3 lysine 14) acetylation and cell proliferation. Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Acts as a coactivator of the licensing factor CDT1. Specifically represses AR-mediated transcription.
http://www.uniprot.org/uniprot/Q810T5
Acetylation is essential for nuclear heme oxygenase-1-enhanced tumor growth and invasiveness<...
TY - JOUR. T1 - Acetylation is essential for nuclear heme oxygenase-1-enhanced tumor growth and invasiveness. AU - Hsu, F. F.. AU - Chiang, M. T.. AU - Li, F. A.. AU - Yeh, C. T.. AU - Lee, W. H.. AU - Chau, L. Y.. N1 - Funding Information: This work was supported by the Ministry of Science and Technology Taiwan (MOST 103-2320-B-001-013-MY3). We thank IBMS proteomics core for the LC-MS analysis.. PY - 2017/8/28. Y1 - 2017/8/28. N2 - Overexpression of heme oxygenase-1 (HO-1), an endoplasmic reticulum-anchored enzyme, is observed in many cancers. HO-1 nuclear translocation has been shown to correlate with progression of several cancers. We recently reported that HO-1 is susceptible to intramembrane proteolysis and translocates to the nucleus to promote cancer growth and invasiveness without depending on its enzymatic activity. In the present study, we show that the HO-1 lacking C-terminal transmembrane segment (t-HO-1) was susceptible to acetylation by p300 and CREB-binding protein (CBP) histone ...
https://tmu.pure.elsevier.com/en/publications/acetylation-is-essential-for-nuclear-heme-oxygenase-1-enhanced-tu
Histone deacetylation during brain development is essential for permanent masculinization of sexual behavior.
Epigenetic histone modifications are emerging as important mechanisms for conveyance of and maintenance of effects of the hormonal milieu to the developing brain. We hypothesized that alteration of histone acetylation status early in development by s
http://www.biomedsearch.com/nih/Histone-Deacetylation-during-Brain-Development/21586557.html
ACETYLATION OF TYROSINE IN PEPSIN | JGPACETYLATION OF TYROSINE IN PEPSIN | JGP
Crystalline 60 per cent active acetyl pepsin has 7 acetyl groups per mol of pepsin, 3 of which are readily hydrolyzed in acid at pH 0.0 or in weak alkali at pH 10.0.. The tyrosine-tryptophane content of this acetylated pepsin, measured colorimetrically, is less than pepsin by three tyrosine equivalents.. Hydrolysis at pH 0.0 or pH 10.0 of the 3 acetyl groups results in a concomitant increase in the number of tyrosine equivalents. In the pH 0.0 hydrolysis experiment there is also a simultaneous increase in specific activity.. The phenol group of glycyl tyrosine is acetylated by ketene under the conditions used in the acetylation of pepsin and the effect of pH on the rate of acetylation is similar in the two cases.. It is concluded that the acetyl groups in the 60 per cent active acetyl pepsin, which are responsible for the decrease in specific enzymatic activity, are 3 in number and are attached to 3 tyrosine phenol groups of the pepsin molecule.. ...
http://jgp.rupress.org/content/19/2/283
Acetylome in Human Fibroblasts From Parkinsons Disease PatientsAcetylome in Human Fibroblasts From Parkinsons Disease Patients
Parkinsons disease (PD) is amultifactorial neurodegenerative disorder. The pathogenesis of this disease is associated with gene and environmental factors. Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most frequent genetic cause of familial and sporadic PD. Moreover, posttranslational modifications, including protein acetylation, are involved in the molecular mechanism of PD. Acetylation of lysine proteins is a dynamic process that ismodulated in PD. In this descriptive study, we characterized the acetylated proteins and peptides in primary fibroblasts from idiopathic PD (IPD) and genetic PD harboring G2019S or R1441G LRRK2 mutations. Identified acetylated peptides are modulated between individuals groups. Although acetylated nuclear proteins are the most represented in cells, they are hypoacetylated in IPD. Results display that the level of hyperacetylated and hypoacetylated peptides are, respectively, enhanced in genetic PD and in IPD cells ...
https://addi.ehu.es/handle/10810/30349
The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. - Immunology
The tumor-suppressor function of p53 relies on its transcriptional activity, which is modulated by post-translational modifications and interactions with regulatory proteins. The prolyl isomerase Pin1 has a central role in transducing phosphorylation of p53 into conformational changes that affect p53 stability and function. We found that Pin1 is required for efficient loading of p53 on target promoters upon stress. In addition, Pin1 is recruited to chromatin by p53 and stimulates binding of the p300 acetyltransferase and consequent p53 acetylation. Accordingly, tumor-associated mutations at Pin1-binding residues within the p53 proline-rich domain hamper acetylation of p53 by p300. After phosphorylation of p53 at Ser46 triggered by cytotoxic stimuli, Pin1 also mediates p53s dissociation from the apoptosis inhibitor iASPP, promoting cell death. In tumors bearing wild-type p53, expression of Pin1 and iASPP are inversely correlated, supporting the clinical relevance of these interactions.
https://www.immunology.ox.ac.uk/publications/84044
AMPK promotes p53 acetylation via phosphorylation and inactivation of SIRT1 in liver cancer cells - Research Database, The...
AMP-activated protein kinase (AMPK), a biologic sensor for cellular energy status, has been shown to act upstream and downstream of known tumor suppressors. However, whether AMPK itself plays a tumor suppressor role in cancer remains unclear. Here, we found that the a2 catalytic subunit isoform of AMPK is significantly downregulated in hepatocellular carcinoma (HCC). Clinicopathologic analysis revealed that underexpression of AMPK-a2 was statistically associated with an undifferentiated cellular phenotype and poor patient prognosis. Loss of AMPK-a2 in HCC cells rendered them more tumorigenic than control cells both in vitro and in vivo. Mechanistically, ectopic expression of AMPK enhanced the acetylation and stability of p53 in HCC cells. The p53 deacetylase, SIRT1, was phosphorylated and inactivated by AMPK at Thr344, promoting p53 acetylation and apoptosis of HCC cells. Taken together, our findings suggest that underexpression of AMPK is frequently observed in HCC, and that inactivation of ...
http://discovery.dundee.ac.uk/portal/en/research/ampk-promotes-p53-acetylation-via-phosphorylation-and-inactivation-of-sirt1-in-liver-cancer-cells
Acetyl-CoA Carboxylase 1-Dependent Protein Acetylation Controls Breast Cancer Metastasis and Recurrence | SFB 1321Acetyl-CoA Carboxylase 1-Dependent Protein Acetylation Controls Breast Cancer Metastasis and Recurrence | SFB 1321
Breast tumor recurrence and metastasis represent the main causes of cancer-related death in women, and treatments are still lacking. Here, we define the lipogenic enzyme acetyl-CoA carboxylase (ACC) 1 as a key player in breast cancer metastasis. ACC1 phosphorylation was increased in invading cells both in murine and human breast cancer, serving as a point of convergence for leptin and transforming growth factor (TGF) β signaling. ACC1 phosphorylation was mediated by TGFβ-activated kinase (TAK) 1, and ACC1 inhibition was indispensable for the elevation of cellular acetyl-CoA, the subsequent increase in Smad2 transcription factor acetylation and activation, and ultimately epithelial-mesenchymal transition and metastasis induction. ACC1 deficiency worsened tumor recurrence upon primary tumor resection in mice, and ACC1 phosphorylation levels correlated with metastatic potential in breast and lung cancer patients. Given the demonstrated effectiveness of anti-leptin receptor antibody treatment in ...
https://sfb1321.med.tum.de/en/publications/acetyl-coa-carboxylase-1-dependent-protein-acetylation-controls-breast-cancer
The Histone Acetyltransferase Gcn5 Positively Regulates T Cell Activation | The Journal of ImmunologyThe Histone Acetyltransferase Gcn5 Positively Regulates T Cell Activation | The Journal of Immunology
Based on our discoveries, we propose a model for Gcn5 regulation of T cell activation: when stimulated by specific Ags, TCR signaling induces the nuclear translocation of NFAT. NFAT then binds to and recruits Gcn5 to the il-2 promoter, where it promotes H3K9 acetylation to activate il-2 gene transcription. Suppression of Gcn5 functions inhibits T cell activation, suggesting that Gcn5 may provide a potential therapeutic target for treatment of autoimmune disease. Therefore, GCN5 is a positive regulator of T cell activation and CD4 T cell differentiation into Th1 and Th17. This conclusion is supported by the following observations: 1) the targeted Gcn5 deletion specifically in T cells partially blocks T cell development at the stage from DN2 to DN3 transition; 2) loss of Gcn5 function attenuates T cell activation in vitro and in vivo; 3) Gcn5 is recruited onto the il-2 promoter through NFAT interaction to modify H3K9 acetylation; and 4) T cell-specific Gcn5 gene deletion largely protects mice from ...
https://www.jimmunol.org/content/198/10/3927
Histone H4 (1-21), p300/CBP SubstrateHistone H4 (1-21), p300/CBP Substrate
Peptides , Histones , Histone H4 Peptides , Histone H4 (1-21), p300/CBP Substrate; This sequence is amino acids 1 to 21 fragment of the histone 4. Histone acetyltransferase (HAT) p300/CBP catalyses the acetylation of this N-terminal tail of free histone H4 at Lys5, 8, 12, 16. Lys8 is thought to be the preferred acetylation site in H4. Determinants of interaction of p300 with histone 4 appear to be fully present on H4-21, the N-terminal region of the protein.; SGRGKGGKGLGKGGAKRHRKV; H-Ser-Gly-Arg-Gly-Lys-Gly-Gly-Lys-Gly-Leu-Gly-Lys-Gly-Gly-Ala-Lys-Arg-His-Arg-Lys-Val-OH
https://www.anaspec.com/products/product.asp?id=45151&productid=26699
Epigenetic Regulation of Spinal CXCR2 Signaling in Incisional Hypersensitivity in Mice | Anesthesiology | ASA PublicationsEpigenetic Regulation of Spinal CXCR2 Signaling in Incisional Hypersensitivity in Mice | Anesthesiology | ASA Publications
Histone acetylation along with DNA methylation is the main epigenetic process that influences nociceptive gene expression in pain states identified to this point. Transcription-restrictive heterochromatin is converted to transcription-permissive euchromatin by histone acetylation and results in enhanced gene expression. Recently, several pain-related chemokine receptors and its relevant ligands are demonstrated to be epigenetically regulated via histone acetylation.13,44-46 The chemokine CCL2/monocyte chemoattractant protein-1 was shown separately to contribute to mechanical sensitization after surgical incision.45 Likewise, CXCR2 was of particular interest to us as this gene has been linked to nociceptive sensitization in several other studies.13,18,19 In the current study, we showed that both CXCR2 and its ligand KC were significantly increased in spinal cord tissue after incision when histone deacetylation was blocked. Furthermore, the relevant population of CXCR2 receptors probably resides ...
http://anesthesiology.pubs.asahq.org/article.aspx?articleid=1918104
Simultaneous detection of site-specific histone methylations and acetylation assisted by single template oriented molecularly...Simultaneous detection of site-specific histone methylations and acetylation assisted by single template oriented molecularly...
Histone post-translational modification (PTM) is a marker for gene transcription and is involved in a range of cancers, such as breast cancer. Most importantly, different modifications of a specific site (e.g., mono-, di- and tri-methylations and acetylation at a lysine residue) are individually enriched in particu
https://pubs.rsc.org/en/content/articlelanding/2020/an/c9an02360g/unauth
Assays for Acetylation and Other Acylations of Lysine Residues - Current ProtocolsAssays for Acetylation and Other Acylations of Lysine Residues - Current Protocols
Lysine acetylation refers to addition of an acetyl moiety to the epsilon‐amino group of a lysine residue and is important for regulating protein functions in various organisms from bacteria to humans
http://www.currentprotocols.com/WileyCDA/CPUnit/refId-ps1411.html
EHD3EHD3
"Acetylation". www.uniprot.org. Retrieved 2016-10-16. Chukkapalli S, Amessou M, Dekhil H, Dilly AK, Liu Q, Bandyopadhyay S, ... The EHD3 protein suffers three kinds of amino acid modifications: Acetylation. It consists of attaching an acetyl group at the ...
https://en.wikipedia.org/wiki/EHD3
Rockefeller UniversityRockefeller University
Eugene Russo (March 1, 1999). "Acetylation". The Scientist. Retrieved November 1, 2021. Jennifer Fisher Wilson (January 9, 2020 ...
https://en.wikipedia.org/wiki/Rockefeller_University
JAK-STAT signaling pathwayJAK-STAT signaling pathway
STAT2 acetylation is important for interactions with other STATs, and for the transcription of anti-viral genes. Acetylation of ... and IL-6 JAK-STAT pathways that use STAT3 require acetylation for transcription of IL-6 response genes. STAT5 acetylation on ... Acetylation. STAT1, STAT2, STAT3, STAT5 and STAT6 have been shown to be acetylated. STAT1 may have an acetyl group attached to ... Zhuang, Shougang (2013). "Regulation of STAT signaling by acetylation". Cellular Signalling. 25 (9): 1924-1931. doi:10.1016/j. ...
https://en.wikipedia.org/wiki/JAK-STAT_signaling_pathway
N-acetyltransferaseN-acetyltransferase
NATs catalyze the acetylation of small molecules through a double displacement reaction called the ping pong bi bi reaction. ... Evans, D.A.; White, T.A. (1964). "Human acetylation polymorphism". J. Lab. Clin. Med. 63: 394-403. PMID 14164493. Hein, D.W.; ... These polymorphisms modify the stability and/ or catalytic activity of enzymes that alter acetylation rates for drugs and ... Hein, D.W. (2000). "Molecular genetics and epidemiology of the NAT1 and NAT2 acetylation polymorphisms". Cancer Epidemiol. ...
https://en.wikipedia.org/wiki/N-acetyltransferase
H4K8acH4K8ac
... indicates acetylation of lysine 8 on histone H4 protein subunit: The genomic DNA of eukaryotic cells is wrapped around ... In histone acetylation and deacetylation, histone proteins are acetylated and deacetylated on lysine residues in the N-terminal ... The histone mark acetylation can be detected in a variety of ways: 1. Chromatin Immunoprecipitation Sequencing (ChIP-sequencing ... Histone acetylation Huang, Suming; Litt, Michael D.; Ann Blakey, C. (2015-11-30). Epigenetic Gene Expression and Regulation. pp ...
https://en.wikipedia.org/wiki/H4K8ac
2-Nitroaniline2-Nitroaniline
Acetylation affords 2-nitroacetanilide. 3-Nitroaniline 4-Nitroaniline Safety data for o-nitroaniline Gerald Booth (2007). " ...
https://en.wikipedia.org/wiki/2-Nitroaniline
Acetoacetate decarboxylaseAcetoacetate decarboxylase
Selective acetylation of enzyme". Biochemistry. 7 (3): 913-919. doi:10.1021/bi00843a005. Schmidt, Donald E.; F.H. Westheimer ( ...
https://en.wikipedia.org/wiki/Acetoacetate_decarboxylase
H3K56acH3K56ac
Histones, however, are not the only proteins regulated by posttranslational acetylation. H3K56ac indicates acetylation of ... It is a mark that indicates the acetylation at the 56th lysine residue of the histone H3 protein. It is a covalent modification ... In histone acetylation and deacetylation, histone proteins are acetylated and deacetylated on lysine residues in the N-terminal ... H3T45P promotes H3K56 acetylation. Phosphorylation of the nucleosome DNA entry-exit region improves access to DNA binding ...
https://en.wikipedia.org/wiki/H3K56ac
KAT2AKAT2A
Col E, Gilquin B, Caron C, Khochbin S (2002). "Tat-controlled protein acetylation". J. Biol. Chem. 277 (40): 37955-60. doi: ... Randhawa GS, Bell DW, Testa JR, Feinberg AP (1998). "Identification and mapping of human histone acetylation modifier gene ... "UV-damaged DNA-binding protein in the TFTC complex links DNA damage recognition to nucleosome acetylation". EMBO J. 20 (12): ... "UV-damaged DNA-binding protein in the TFTC complex links DNA damage recognition to nucleosome acetylation". EMBO J. 20 (12): ...
https://en.wikipedia.org/wiki/KAT2A
H4K12acH4K12ac
... indicates acetylation of lysine 12 on histone H4 protein subunit: The genomic DNA of eukaryotic cells is wrapped around ... Acetylation of histone H4K5 and H4K12 is enriched at centromeres. H4K8ac and H4K12ac are associated with active promoters to ... In histone acetylation and deacetylation, histone proteins are acetylated and deacetylated on lysine residues in the N-terminal ... It is a mark that indicates the acetylation at the 12th lysine residue of the histone H4 protein. H4K12ac is involved in ...
https://en.wikipedia.org/wiki/H4K12ac
Arylamine N-acetyltransferaseArylamine N-acetyltransferase
Tabor H, Mehler AH, Stadtman ER (1953). "The enzymatic acetylation of amines". J. Biol. Chem. 204 (1): 127-138. PMID 13084583. ... Weissbach H, Redfield BG, Axelrod J (1961). "The enzymic acetylation of serotonin and other naturally occurring amines". ...
https://en.wikipedia.org/wiki/Arylamine_N-acetyltransferase
Zinc finger protein 226Zinc finger protein 226
... or by acetylation of a C-terminus lysine. Acetylation in other zinc finger proteins, such as GATA1, are known to enhance their ... Acetylation was another modification identified. In the case of promyelocytic leukemia, a condition resulting in the abundance ...
https://en.wikipedia.org/wiki/Zinc_finger_protein_226
Protein biosynthesisProtein biosynthesis
Histones undergo acetylation on their lysine residues by enzymes known as histone acetyltransferase. The effect of acetylation ... Histone-based regulation of DNA transcription is also modified by acetylation. Acetylation is the reversible covalent addition ... Drazic A, Myklebust LM, Ree R, Arnesen T (October 2016). "The world of protein acetylation". Biochimica et Biophysica Acta (BBA ... Examples of processes which add chemical groups to the target protein include methylation, acetylation and phosphorylation. ...
https://en.wikipedia.org/wiki/Protein_biosynthesis
H4K5acH4K5ac
Acetylation of histone H4K5 and H4K12ac is enriched at centromeres. H4K5ac indicates acetylation of lysine 5 on histone H4 ... It is a mark that indicates the acetylation at the 5th lysine residue of the histone H4 protein. H4K5 is the closest lysine ... In histone acetylation and deacetylation, histone proteins are acetylated and deacetylated on lysine residues in the N-terminal ... The histone mark acetylation can be detected in a variety of ways: 1. Chromatin Immunoprecipitation Sequencing (ChIP-sequencing ...
https://en.wikipedia.org/wiki/H4K5ac
H3T45PH3T45P
H3T45 phosphorylation promotes H3K56 acetylation. Phosphorylation of the nucleosome DNA entry-exit region improves access to ... Thus results suggested that the acetylation of histones can stimulate transcription by suppressing an inhibitory ... and the combination of phosphorylation and acetylation has the ability to alter DNA accessibility to transcription regulatory ... "Protein kinase C coordinates histone H3 phosphorylation and acetylation". eLife. 4: e09886. doi:10.7554/eLife.09886. PMC ...
https://en.wikipedia.org/wiki/H3T45P
Carnitine O-acetyltransferaseCarnitine O-acetyltransferase
Friedman S, Fraenkel G (Dec 1955). "Reversible enzymatic acetylation of carnitine". Archives of Biochemistry and Biophysics. 59 ...
https://en.wikipedia.org/wiki/Carnitine_O-acetyltransferase
O-ToluidineO-Toluidine
... including N-acetylation, N-oxidation and N-hydroxylation, and ring oxidation. 4-Hydroxylation and N-acetylation of toluidine ... N-acetylation is also demonstrated. Prilocaine, an amino amide-type local anesthetic, yields o-toluidine when metabolized by ... conjugates in an acidic urine environment to either react directly with DNA or be bio-activated via sulfation or acetylation by ...
https://en.wikipedia.org/wiki/O-Toluidine
PrimordiumPrimordium
https://doi.org/10.4161/psb.6.11.17506 Liu, X., Yang, S., Yu, C.-W., Chen, Y., Wu, K. (2016). Histone Acetylation and Plant ...
https://en.wikipedia.org/wiki/Primordium
Coiled-coil domain containing 74aCoiled-coil domain containing 74a
Drazic A, Myklebust LM, Ree R, Arnesen T (October 2016). "The world of protein acetylation". Biochimica et Biophysica Acta (BBA ... Drazic A, Myklebust LM, Ree R, Arnesen T (October 2016). "The world of protein acetylation". Biochimica et Biophysica Acta (BBA ... Additionally, SUMOylation, methylation, and acetylation sites are predicted within highly conserved regions and may play a part ...
https://en.wikipedia.org/wiki/Coiled-coil_domain_containing_74a
Alpha-tubulin N-acetyltransferaseAlpha-tubulin N-acetyltransferase
Tubulin acetylation is one of the signaling pathways for Na+ and K+-ATPase activity. Tubulin acetylation is also involved in ... Tubulin acetylation by ATAT1 has been shown to be elevated by the cell exposure to UV irradiation, as well as its exposure to ... The acetylation is used y the cell as a marker for these stable microtubules. ATAT1 specifically acetylates 'Lys-40' in alpha ... There are some cases in which the mutation of the gene might cause a reduction in the acetylation of the microtubules. Like for ...
https://en.wikipedia.org/wiki/Alpha-tubulin_N-acetyltransferase
TMEM171TMEM171
"N-Acetylation and initial methionine predictor". Terminus. "NetNGlyc 1.0 Server". DTU Bioinformatics: Department of Bio and ... TMEM171 undergoes methionine cleavage and N-terminal acetylation, which is one of the most common modifications of eukaryotic ...
https://en.wikipedia.org/wiki/TMEM171
HIST1H4IHIST1H4I
Turner BM, O'Neill LP, Allan IM (1989). "Histone H4 acetylation in human cells. Frequency of acetylation at different sites ... 2001). "Acetylation of HIV-1 Tat by CBP/P300 increases transcription of integrated HIV-1 genome and enhances binding to core ... Lusic M, Marcello A, Cereseto A, Giacca M (2004). "Regulation of HIV-1 gene expression by histone acetylation and factor ...
https://en.wikipedia.org/wiki/HIST1H4I
H4K91acH4K91ac
... indicates acetylation of lysine 91 on histone H4 protein subunit: The genomic DNA of eukaryotic cells is wrapped around ... In histone acetylation and deacetylation, histone proteins are acetylated and deacetylated on lysine residues in the N-terminal ... Histone code Histone acetylation Huang, Suming; Litt, Michael D.; Ann Blakey, C. (2015-11-30). Epigenetic Gene Expression and ... It is a mark that indicates the acetylation at the 91st lysine residue of the histone H4 protein. No known diseases are ...
https://en.wikipedia.org/wiki/H4K91ac
FAM149AFAM149A
NetAcet: Predicts N-terminal acetylation sites. Here are the results: According to NetAcet, there are no N-terminal acetylation ...
https://en.wikipedia.org/wiki/FAM149A
LagochilinLagochilin
"Investigation of the Lagochiline Acetylation Reaction". Khimiya Prirodnykh Soedinenii. 1: 46-9. Chizhov OS, Kessenikh AV, ...
https://en.wikipedia.org/wiki/Lagochilin
Acetic anhydrideAcetic anhydride
It is also used as an active modification agent via autoclave impregnation and subsequent acetylation to make a durable and ... Similarly it is used in the production of aspirin (acetylsalicylic acid), which is prepared by the acetylation of salicylic ... Acetic anhydride is a versatile reagent for acetylations, the introduction of acetyl groups to organic substrates. In these ... In starch industry, acetic anhydride is a common acetylation compound, used for the production of modified starches (E1414, ...
https://en.wikipedia.org/wiki/Acetic_anhydride
Acetyl-CoAAcetyl-CoA
This acetylation is catalyzed by acetyltransferases. This acetylation affects cell growth, mitosis, and apoptosis. Allosteric ... Melatonin synthesis Acetylation Acetyl-CoA is also the source of the acetyl group incorporated onto certain lysine residues of ... Fritz Lipmann won the Nobel Prize in 1953 for his discovery of the cofactor coenzyme A. The acetylation of CoA is determined by ... Ethanol also serves as a carbon source for acetylation of CoA utilizing the enzyme alcohol dehydrogenase. Degradation of ...
https://en.wikipedia.org/wiki/Acetyl-CoA
HistrionicotoxinsHistrionicotoxins
Hydrolysis, reduction and acetylation yielded 136. Formation of a thiolactam followed by condensation with ethyl bromoacetate ...
https://en.wikipedia.org/wiki/Histrionicotoxins
Chromatin remodelingChromatin remodeling
Acetylation - by HAT (histone acetyl transferase); deacetylation - by HDAC (histone deacetylase) Acetylation tends to define ... On the contrary, histone acetylation relaxes chromatin condensation and exposes DNA for TF binding, leading to increased gene ... These enzymatic modifications include acetylation, methylation, phosphorylation, and ubiquitination and primarily occur at N- ... "Histone deacetylase inhibitor selectively induces p21WAF1 expression and gene-associated histone acetylation". Proceedings of ...
https://en.wikipedia.org/wiki/Chromatin_remodeling
Histone codeHistone code
Acetylation-by HAT (histone acetyl transferase); deacetylation-by HDAC (histone deacetylase): Acetylation tends to define the ' ... Similarly, the combination of phosphorylation of serine residue 10 and acetylation of a lysine residue 14 on histone H3 is a ... Liang, G (2004). "Distinct localization of histone H3 acetylation and H3-K4 methylation to the transcription start sites in the ... 2008). "Combinatorial patterns of histone acetylations and methylations in the human genome". Nat Genet. 40 (7): 897-903. doi: ...
https://en.wikipedia.org/wiki/Histone_code
Acetylation targets mutant huntingtin to autophagosomes for degradationAcetylation targets mutant huntingtin to autophagosomes for degradation
... by acetylation at lysine residue 444 (K444). Increased acetylation at K444 facilitates trafficking of mutant Htt into ... Acetylation targets mutant huntingtin to autophagosomes for degradation Cell. 2009 Apr 3;137(1):60-72. doi: 10.1016/j.cell. ... These studies identify acetylation as a mechanism for removing accumulated protein in HD, and more broadly for actively ... In contrast, mutant Htt that is rendered resistant to acetylation dramatically accumulates and leads to neurodegeneration in ...
https://pubmed.ncbi.nlm.nih.gov/19345187/?dopt=Abstract
Structural Basis for Substrate-specific Acetylation of Nα-acetyltransferase Ard1 from Sulfolobus solfataricus | Scientific...Structural Basis for Substrate-specific Acetylation of Nα-acetyltransferase Ard1 from Sulfolobus solfataricus | Scientific...
... so SsArd1 alone may be responsible for the protein acetylation. Limited proteomic survey of N-terminal acetylation in S. ... Structural Basis for Substrate-specific Acetylation of Nα-acetyltransferase Ard1 from Sulfolobus solfataricus. *Yu-Yung Chang1 ... Protein Nα-acetylation is a ubiquitous post-translational modification present from archaea to mammalian cells and is involved ... Structural Basis for Substrate-specific Acetylation of Nα-acetyltransferase Ard1 from Sulfolobus solfataricus. Sci Rep 5, 8673 ...
https://www.nature.com/articles/srep08673?error=cookies_not_supported&code=852bc8c4-3756-46e8-97ce-30f4f11eb3a1
New Acetylation of Heterocycles - ChemistryViewsNew Acetylation of Heterocycles - ChemistryViews
Carbonylative Acetylation of Heterocycles,. Youcan Zhang, Zhiping Yin, Xiao-Feng Wu,. European Journal of Organic Chemistry ... New Acetylation of Heterocycles. Author: European Journal of Organic Chemistry. Nitrogen-containing heterocycles have wide ... have developed a procedure for the carbonylative acetylation of heterocycles. The team selected 1-methyl-1H-benzo[d]imidazole ...
https://www.chemistryviews.org/details/ezine/11206566/New_Acetylation_of_Heterocycles/
Small Molecule Modulators of HATs & HDACs | Regulate Histone AcetylationSmall Molecule Modulators of HATs & HDACs | Regulate Histone Acetylation
Histone acetylation is a reversible reaction that occurs on the lysine residues of histone tails. Histone acetyltransferases ( ... Histone acetylation plays an important role in the modulation of chromatin condensation and transcriptional regulation. ...
https://www.activemotif.com/catalog/992/histone-acetylation-hat-hdac
Sciencemadness Discussion Board - N-Acetylation of taurine - Powered by XMB 1.9.11Sciencemadness Discussion Board - N-Acetylation of taurine - Powered by XMB 1.9.11
N-Acetylation of taurine. Heres taurine:. and heres acamprosate, its acetylated analogue:. From what I read, esters can be used ... Sciencemadness Discussion Board » Fundamentals » Beginnings » N-Acetylation of taurine. Select A Forum. Fundamentals. » ... Sciencemadness Discussion Board » Fundamentals » Beginnings » N-Acetylation of taurine. Select A Forum. Fundamentals. » ...
https://www.sciencemadness.org/whisper/viewthread.php?tid=23976#pid281010
Influence of Acetylation on Fire Resistance of Beech PlywoodInfluence of Acetylation on Fire Resistance of Beech Plywood
... IRG/WP 06-40326. B Mohebby, A Talaii, A Karimi , S Kazemi Najafi ... Influence of acetylation on fire resistance was studied in beech plywood. Beech layers were acetylated in a reactor with acetic ... Keywords: acetylation, fire resistance, plywood, ignition, glowing, oriental beech, Fagus orientalis Conference: 06-06-18/22 ... This study revealed that the acetylation retards slightly fire in plywood; however it does not resist wood against fire. ...
https://www.irg-wp.com/irgdocs/details.php?d7b99e7e-d1e9-4b38-b1f6-7a93e1788061
Acetylation-dependent nuclear arrangement and recruitment of BMI1 protein to UV-damaged chromatin | Masaryk UniversityAcetylation-dependent nuclear arrangement and recruitment of BMI1 protein to UV-damaged chromatin | Masaryk University
Acetylation-dependent nuclear arrangement and recruitment of BMI1 protein to UV-damaged chromatin. Authors. ŠUSTÁČKOVÁ Gabriela ... Here, we asked whether acetylation can influence the nuclear arrangement and function of the BMI1 protein, a core component of ... Acetylation-dependent nuclear arrangement and recruitment of BMI1 protein to UV-damaged chromatin ... and the nuclear arrangement of BMI1 protein can be influenced by acetylation and occur as an early event prior to the ...
https://www.muni.cz/en/research/publications/952094
Determinants within the C-Terminal Domain of Streptomyces lividans Acetyl-CoA Synthetase that Block Acetylation of Its Active...Determinants within the C-Terminal Domain of Streptomyces lividans Acetyl-CoA Synthetase that Block Acetylation of Its Active...
Reversible lysine acetylation (RLA) is a widespread regulatory mechanism that modulates the function of proteins involved in ... Finally, we suggest that acetylation of SlAcs depends on factors or conditions other than those present in our in vitro system ... Determinants within the C-Terminal Domain of Streptomyces lividans Acetyl-CoA Synthetase that Block Acetylation of Its Active ... "Determinants Within the C-Terminal Domain of Streptomyces Lividans Acetyl-CoA Synthetase That Block Acetylation of Its Active ...
https://dspace.mit.edu/handle/1721.1/89457
Acetylation of acetylhydrazine, the toxic metabolite of isoniazid, in humans. Inhibition by concomitant administration of...Acetylation of acetylhydrazine, the toxic metabolite of isoniazid, in humans. Inhibition by concomitant administration of...
Acetylation of acetylhydrazine, the toxic metabolite of isoniazid, in humans. Inhibition by concomitant administration of ... Acetylation of acetylhydrazine, the toxic metabolite of isoniazid, in humans. Inhibition by concomitant administration of ... Acetylation of acetylhydrazine, the toxic metabolite of isoniazid, in humans. Inhibition by concomitant administration of ... Acetylation of acetylhydrazine, the toxic metabolite of isoniazid, in humans. Inhibition by concomitant administration of ...
https://jpet.aspetjournals.org/content/243/2/686
AID 1270697 - Inhibition of SIRT2 in human HCT116 cells assessed as increase in alpha-tubulin acetylation up to 50 uM incubated...AID 1270697 - Inhibition of SIRT2 in human HCT116 cells assessed as increase in alpha-tubulin acetylation up to 50 uM incubated...
Inhibition of SIRT2 in human HCT116 cells assessed as increase in alpha-tubulin acetylation up to 50 uM incubated for 24 hrs by ...
https://pubchem.ncbi.nlm.nih.gov/bioassay/1270697
EpiQuik Global Histone H3 Acetylation Assay Kit | LAB MARKEpiQuik Global Histone H3 Acetylation Assay Kit | LAB MARK
A convenient package of tools that allows the experimenter to measure global acetylation of histone H3 at tremendously fast ... The EpiQuik Global Histone H3 Acetylation Assay Kit is suitable for specifically measuring global histone H3 acetylation using ... A convenient package of tools that allows the experimenter to measure global acetylation of histone H3 at tremendously fast ...
https://www.labmark.eu/epiquik-global-histone-h3-acetylation-assay-kit
Journal name: Journal of proteomics / Subject: aspirin and acetylation / Subject term: cardiovascular diseases - PubAg Search...Journal name: Journal of proteomics / Subject: aspirin and acetylation / Subject term: cardiovascular diseases - PubAg Search...
... acetylation Remove constraint Subject: acetylation Subject term cardiovascular diseases Remove constraint Subject term: ... 1. A high glucose level is associated with decreased aspirin-mediated acetylation of platelet cyclooxygenase (COX)-1 at serine ... acetylation; aspirin; blood platelets; cardiovascular diseases; diabetes; glucose; glycation; hyperglycemia; mass spectrometry ...
https://pubag.nal.usda.gov/?f%5Bjournal_name%5D%5B%5D=Journal+of+proteomics&f%5Bsubject_facet%5D%5B%5D=aspirin&f%5Bsubject_facet%5D%5B%5D=acetylation&f%5Bsubject_term%5D%5B%5D=cardiovascular+diseases&per_page=20&sort=relevance
Nuclear Arc Interacts with the Histone Acetyltransferase Tip60 to Modify H4K12 Acetylation | eNeuroNuclear Arc Interacts with the Histone Acetyltransferase Tip60 to Modify H4K12 Acetylation | eNeuro
In older mice, only acetylation of H4K12 failed to increase, suggesting that the loss of histone H4K12 acetylation is ... YFP did not affect H4K12 acetylation, while Arc-YFP over-expression increased H4K12 acetylation in both scenarios. However, the ... Overexpression of YFP did not affect H4K12 acetylation, whereas Arc-YFP significantly increased H4K12 acetylation (Fig. 10B). ... Alarcon JM, Malleret G, Touzani K, Vronskaya S, Ishii S Kandel ER, Barco A (2004) Chromatin acetylation, memory, and LTP are ...
https://www.eneuro.org/content/1/1/ENEURO.0019-14.2014.long
JTW Seminar | Timothy A. McKinsey | Protein acetylation in heart failure: from the epigenome to sarcomeres - The Texas Heart...JTW Seminar | Timothy A. McKinsey | 'Protein acetylation in heart failure: from the epigenome to sarcomeres' - The Texas Heart...
JTW Seminar , Timothy A. McKinsey , "Protein acetylation in heart failure: from the epigenome to sarcomeres". February 14, 2019 ...
https://www.texasheart.org/event/jtw-seminar-timothy-mckinsey/
A MnSOD Acetylation Mutant Mouse for Luminal B Endocrine Resistant Breast Cancer - Northwestern Scholars
A MnSOD Acetylation Mutant Mouse for Luminal B Endocrine Resistant Breast Cancer. *Gius, David R (PD/PI) ...
https://www.scholars.northwestern.edu/en/projects/a-mnsod-acetylation-mutant-mouse-for-luminal-b-endocrine-resistan-4
KOPS.Unnatural amino acids to study site-specific protein acetylationKOPS.Unnatural amino acids to study site-specific protein acetylation
Acetylation, Ubiquitin, Ubiquitin acetylation, noncanonical amino acids, acetyllysine, triflouroacetyllysine, ketolysine. ... Unnatural amino acids to study site-specific protein acetylation. Restricted until:. Feb 14, 2024. ... phdthesis{Kienle2021Unnat-56556, title={Unnatural amino acids to study site-specific protein acetylation}, year={2021}, author ... Unnatural amino acids to study site-specific protein acetylation [Dissertation]. Konstanz: University of Konstanz ...
https://kops.uni-konstanz.de/handle/123456789/56556
TDP-43 acetylation as a pathogenic modification in ALS & related proteinopathies - JPNDTDP-43 acetylation as a pathogenic modification in ALS & related proteinopathies - JPND
... acetylation?biology,?I?previously?demonstrated?that?acetylation?of?the? tau? protein? promotes? tangle? formation? in? ... Home » Database » TDP-43 acetylation as a pathogenic modification in ALS & related proteinopathies ... protein TDP-43, DNA-Binding Proteins, Amyotrophic Lateral Sclerosis, Acetylation, Frontotemporal Lobar Degenerations ... acetylation?in?causing?impaired?TDP? 43?binding?to?target?genes?and?RNAs,?leading?to?a?TDP?43?loss?of?function.??Finally,?as?an ...
https://www.neurodegenerationresearch.eu/survey/tdp-43-acetylation-as-a-pathogenic-modification-in-als-related-proteinopathies-2/
Missing value imputation for microarray gene expression data using histone acetylation information | BMC Bioinformatics | Full...Missing value imputation for microarray gene expression data using histone acetylation information | BMC Bioinformatics | Full...
It incorporates the histone acetylation information into the conventional KNN(k-nearest neighbor) and LLS(local least square) ... We demonstrated that the using of histone acetylation information could greatly improve the performance of the imputation ... Moreover, with more knowledge accumulated on gene regulatory mechanism in addition to histone acetylation, the performance of ... The experimental results indicated that the use of acetylation information can provide significant improvements in microarray ...
https://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-9-252
The discovery of the ER-based acetylation machinery: from aging to Alzheimer's disease … cancer too? - Department of Physics -...
The discovery of the ER-based acetylation machinery: from aging to Alzheimers disease ... cancer too?. Date: Tuesday, ... Here, I will describe the biochemical properties of the individual components of the ER-based acetylation machinery and their ... discuss the initial characterization of a newly-generated animal model showing a possible impact of the ER-based acetylation ...
https://www.physics.wisc.edu/events/?id=2741
A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional...
T1 - A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional ... A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional ... A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional ... title = "A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and ...
https://pennstate.pure.elsevier.com/en/publications/a-subset-of-tafiis-are-integral-components-of-the-saga-complex-re
Flow-enhanced priming of hESCs through H2B acetylation and chromatin decondensation | Stem Cell Research & Therapy | Full TextFlow-enhanced priming of hESCs through H2B acetylation and chromatin decondensation | Stem Cell Research & Therapy | Full Text
Shear flow was able to induce H2B acetylation and nuclear spreading by CFL2/F-actin cytoskeletal reorganization. The resulting ... Functional analyses revealed significant alterations in histone acetylation, nuclear size, and cytoskeleton for hESC under ... Acetylation of histone H2B and chromatin state under fluid shear. Histone acetylation was shown in (a) with typical ... We further tested the H2B acetylation using a typical APKK12(Ac)GSK15(Ac) KAVYC acetylation site of H2B and the chromatin ...
https://stemcellres.biomedcentral.com/articles/10.1186/s13287-019-1454-z
WERAM - Writers, Erasers & Readers of Histone Acetylation and Methylation Database
Histone Acetylation of Coccidioides immitis. There are 18 protein exist in 3 classes. HAT (5). ...
http://weram.biocuckoo.org/species.php?spe=Coccidioides_immitis
BRPF3-HBO1 regulates replication origin activation and histone H3K14 acetylation<...
BRPF3-HBO1 regulates replication origin activation and histone H3K14 acetylation. In: EMBO Journal. 2016 ; Vol. 35, No. 2. pp. ... BRPF3-HBO1 regulates replication origin activation and histone H3K14 acetylation. EMBO Journal. 2016 Jan 1;35(2):176-192. doi: ... BRPF3-HBO1 regulates replication origin activation and histone H3K14 acetylation. Yunpeng Feng, Arsenios Vlassis, Céline Roques ... We thus propose that BRPF3-HBO1 acetylation of histone H3K14 around TSS facilitates efficient activation of nearby replication ...
https://tmu.pure.elsevier.com/en/publications/brpf3-hbo1-regulates-replication-origin-activation-and-histone-h3-2
Epigenetic engineering: histone H3K9 acetylation is compatible with kinetochore structure and function<...
2012). Epigenetic engineering: histone H3K9 acetylation is compatible with kinetochore structure and function. Journal of Cell ... Epigenetic engineering: histone H3K9 acetylation is compatible with kinetochore structure and function. Journal of Cell Science ... Epigenetic engineering: histone H3K9 acetylation is compatible with kinetochore structure and function. In: Journal of Cell ... Dive into the research topics of Epigenetic engineering: histone H3K9 acetylation is compatible with kinetochore structure and ...
https://research.birmingham.ac.uk/en/publications/epigenetic-engineering-histone-h3k9-acetylation-is-compatible-wit
Histone acetylation dynamics modulates chromatin conformation and allele-specific interactions at oncogenic loci - Aix...
Histone acetylation dynamics modulates chromatin conformation and allele-specific interactions at oncogenic loci Stephanie ... Stephanie Sungalee, Yuanlong Liu, Ruxandra Lambuta, Natalya Katanayeva, Maria Donaldson Collier, et al.. Histone acetylation ...
https://hal-amu.archives-ouvertes.fr/hal-03565951
The Quest for Height: Grow Taller | Increase Height | Bone Size: Grow Taller by Increasing Chromatin Acetylation?The Quest for Height: Grow Taller | Increase Height | Bone Size: Grow Taller by Increasing Chromatin Acetylation?
How does chromatin acetylation on SOX9 affect height and how do we increase chromatin acetylation?. Histone acetylation ... Conclusion: Increasing chromatin acetylation may be a way to grow taller if you are deficient. The best way to do so is via BMP ... Tip60 enhances acetylation of Sox9 mainly through K61, 253, 398 residues; however, the K61/253/398A mutant of Sox9 still ... Low levels of chromatin acetylation results in less of the ECM genes aggrecan and COL2A1 which may interfere with optimal ...
http://www.heightquest.com/2011/06/grow-taller-by-increasing-chromatin.html
Lysine acetylation in mitochondria: From inventory to function :: MPG.PuReLysine acetylation in mitochondria: From inventory to function :: MPG.PuRe
Lysine acetylation in mitochondria: From inventory to function ... 2016). Lysine acetylation in mitochondria: From inventory to ...
https://pure.mpg.de/pubman/faces/ViewItemOverviewPage.jsp?itemId=item_2403958_1
Histone acetylationHistone acetylation
Acetylation facilitates transcription by making DNA more accessible to RNA polymerase II. Histone deacetylation is perfomed by ... Histone acetylation: Histone acetyl transferases (HATs) adds acetyl groups to histone tails thus reducing positive charge and ... Histone acetylation. Articles related to Histone acetylation on line retrieved from PubMed Online articles related to Histone ...
https://mpmp.huji.ac.il/maps/Histone.html
Darwins God: Bacterial Protein Acetylation: The Dawning of a New AgeDarwin's God: Bacterial Protein Acetylation: The Dawning of a New Age
With spontaneously you mean without Jesus, right? Jesus woke up one day and decided to add some acetylation capabilities to his ... With evolution we now must believe that even protein acetylation miraculously appeared very early in the history of life. This ... Those astronomically unlikely, incredibly complex, changes must have arisen to create the phenomenal protein acetylation ...
https://darwins-god.blogspot.com/2012/07/bacterial-protein-acetylation-dawning.html?showComment=1343272389697
Expression, localization and acetylation level of heterogeneous ribonucleoproteins (hnRNPs) depending on KRAS mutational status...Expression, localization and acetylation level of heterogeneous ribonucleoproteins (hnRNPs) depending on KRAS mutational status...
In this project we propose to determine expression levels of hnRNPs, degree of acetylation, as well as its subcellular ... Expression, localization and acetylation level of heterogeneous ribonucleoproteins (hnRNPs) depending on KRAS mutational status ... Expression, localization and acetylation level of heterogeneous ribonucleoproteins (hnRNPs) depending on KRAS mutational status ... Expression, localization and acetylation level of heterogeneous ribonucleoproteins (hnRNPs) depending on KRAS mutational status ...
https://riunet.upv.es/handle/10251/51841
HistoneProteinP300-mediated acetylationMethylation and acetylationEpigeneticTranscriptionalSlow acetylationLysine residuesPosttranslational modificationChromatin organizationHistonesProteinsMammalian cellsMechanismRegulationAcetyltransferasesBiologyInhibitionResidues2019IsoniazidBiologicalGenesSubstratesStarchInterfereVitroAromaticMetaboliteAlzheimer'sCellsMechanismsMolecularResultsNuclearDeterminationGreatlyNeurodegenerativeNeurodegenerationProcedureSubject
Histone41
  • Histone acetylation is a reversible reaction that occurs on the lysine residues of histone tails. (activemotif.com)
  • Histone acetylation plays an important role in the modulation of chromatin condensation and transcriptional regulation. (activemotif.com)
  • A convenient package of tools that allows the experimenter to measure global acetylation of histone H3 at tremendously fast speeds and consistency, superior and safer than all other current methods. (labmark.eu)
  • The EpiQuik Global Histone H3 Acetylation Assay Kit is suitable for specifically measuring global histone H3 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells. (labmark.eu)
  • Neuronal activity-induced expression of Arc (1) increases endogenous nuclear Tip60 puncta, (2) recruits Tip60 to PML bodies, and (3) increases histone acetylation of Tip60 substrate H4K12, a learning-induced chromatin modification. (eneuro.org)
  • We present data showing that Arc associates with and enhances Tip60's acetylation of its substrate H4K12, an important learning-induced histone mark. (eneuro.org)
  • An imputation framework called histone acetylation information aided imputation method (HAIimpute method) is presented. (biomedcentral.com)
  • It incorporates the histone acetylation information into the conventional KNN( k -nearest neighbor) and LLS(local least square) imputation algorithms for final prediction of the missing values. (biomedcentral.com)
  • We demonstrated that the using of histone acetylation information could greatly improve the performance of the imputation especially at high missing percentages. (biomedcentral.com)
  • Moreover, with more knowledge accumulated on gene regulatory mechanism in addition to histone acetylation, the performance of our approach can be further improved and verified. (biomedcentral.com)
  • A number of transcriptional coactivator proteins have been identified as histone acetyltransferase (HAT) proteins, providing a direct molecular basis for the coupling of histone acetylation and transcriptional activation. (elsevier.com)
  • Functional analyses revealed significant alterations in histone acetylation, nuclear size, and cytoskeleton for hESC under shear flow. (biomedcentral.com)
  • We thus propose that BRPF3-HBO1 acetylation of histone H3K14 around TSS facilitates efficient activation of nearby replication origins. (elsevier.com)
  • Histone acetylation influences the activity of Sox9-related transcriptional complex. (heightquest.com)
  • The Sox9-related transcriptional apparatus activates its target gene expression through p300-mediated histone acetylation on chromatin. (heightquest.com)
  • Acetylation and Methylation Rapport au Sénat de la commission sur les perturbateurs endocriniens, No public clipboards found for this slide, Histone Modification: Acetylation n Methylation. (rehabsociety.org.hk)
  • Histone Methylation and Acetylation Interactions in DKD. (rehabsociety.org.hk)
  • Histone acetylation and deacetylation are the processes by which the lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. (rehabsociety.org.hk)
  • Unlike acetylation and methylation, histone phosphorylation establishes interactions between other histone modifications and serves as a platform for effector proteins, which leads to a downstream cascade of events. (rehabsociety.org.hk)
  • Histone acetylation maps in aged mice developmentally exposed to lead: epigenetic drift and Alzheimer-related genes Aseel Eid , 1 , 3 Syed Waseem Bihaqi , 3 Christopher Hemme , 2 John M Gaspar , 4 Ronald P Hart , 5 and Nasser H Zawia * 1 , 2 , 3 Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. (rehabsociety.org.hk)
  • Histone acetylation and methylation are the two major modifications that function as a specific transcription regulator in response to various cellular signals. (rehabsociety.org.hk)
  • acetylation and histone H3K4 methylation [17,20]. (rehabsociety.org.hk)
  • 2.2 Histone acetylation and histone methylation 12:38. (rehabsociety.org.hk)
  • Originally reported by Shi and colleagues, lysine specific demethylase 1 (LSD1) was the first histone demethylase described (Shi et … Somanna A. N. Dysregulation of Acetylation Enzymes Inanimal Models of Psychostimulant use Disorders: Evolving Stories. (rehabsociety.org.hk)
  • 50 Years of Histone Acetylation. (bdebate.org)
  • Here we confirm the expression of at least one HDAC protein in Plasmodium falciparum gametocytes and show that histone and nonhistone protein acetylation occurs in this life cycle stage. (edu.au)
  • Here, we show that both classical histone deacetylase HDAC1 and NAD + -dependent deacetylase SIRT1 regulate acetylation level of APE1 and acetylation of APE1 enhances its AP-endonuclease activity both in vitro and in cells. (nebraska.edu)
  • Background: A key finding from recent studies of epigenetic mechanisms of memory is that increasing histone acetylation after a learning experience enhances memory consolidation. (elsevier.com)
  • This has been demonstrated in several preparations, but little is known about whether excitatory and inhibitory memories are equally sensitive to drugs that promote histone acetylation and how transcriptional changes in the hippocampal-medial prefrontal cortex network contribute to these drug effects. (elsevier.com)
  • Levels of histone acetylation and expression of the product of the immediate-early gene c-Fos were assessed by immunohistochemistry following infusion of NaB into the hippocampus (n = 26). (elsevier.com)
  • NaB administered following weak extinction induced behavioral extinction, infralimbic histone acetylation and c-Fos expression consistent with strong extinction. (elsevier.com)
  • Histone acetyltransferases and histone deacetylases (HDACs) determine the acetylation status of histones, regulating gene transcription. (elsevier.com)
  • Taken together, our results suggest that histone acetylation is deeply involved in differentiation of human ESCs and that TSA has a potential as an enhancer of decidualization through promotion of progesterone action. (elsevier.com)
  • Histone Elisa Laboratories manufactures the histone acetylation elisa kit reagents distributed by Genprice. (bioinfogenome.net)
  • The Histone Acetylation Elisa Kit reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (bioinfogenome.net)
  • Description: A sandwich ELISA for quantitative measurement of Rabbit Acetylation Histone H3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (bioinfogenome.net)
  • Histone acetylation is an active mark characteristic of open chromatin , but acetylation on specific lysine residues and histone variants occurs in different biological contexts and can confer various outcomes. (bvsalud.org)
  • Histone modifications, such as methylation and acetylation, or incorporation of histone variants can alter nucleosomal dynamics. (harvard.edu)
  • The enzyme has indeed been shown to catalyse primarily the acetylation of H3 histone with only traces of H4 and H2A/B being acetylated. (or.jp)
  • HDAC inhibition results in widespread alteration of the histone acetylation landscape and BRD4 targeting to gene bodies. (cornell.edu)
  • Histone acetylation, histone methylation, and DNA methylation modifiers are each further stratified into "writers" (w), "editors" (e), and "readers" (r). (who.int)
Protein15
  • Increased acetylation at K444 facilitates trafficking of mutant Htt into autophagosomes, significantly improves clearance of the mutant protein by macroautophagy, and reverses the toxic effects of mutant huntingtin in primary striatal and cortical neurons and in a transgenic C. elegans model of HD. (nih.gov)
  • These studies identify acetylation as a mechanism for removing accumulated protein in HD, and more broadly for actively targeting proteins for degradation by autophagy. (nih.gov)
  • In eukaryotic cells, Nat complexes are composed of one catalytic and one or more auxiliary subunits and they are involved in protein Nα-acetylation. (nature.com)
  • As compared with eukaryotes, in archaea, knowledge of the N-terminal protein acetylation is relative limited 17 . (nature.com)
  • However, no Sulfolobus homolog of the auxiliary subunit of NatA was found, so SsArd1 alone may be responsible for the protein acetylation. (nature.com)
  • Here, we asked whether acetylation can influence the nuclear arrangement and function of the BMI1 protein, a core component of the Polycomb group complex, PRC1. (muni.cz)
  • Our data indicate that the dynamics of recognition of UV-damaged chromatin, and the nuclear arrangement of BMI1 protein can be influenced by acetylation and occur as an early event prior to the recruitment of HPb to UV-irradiated chromatin. (muni.cz)
  • With evolution we now must believe that even protein acetylation miraculously appeared very early in the history of life. (blogspot.com)
  • Those astronomically unlikely, incredibly complex, changes must have arisen to create the phenomenal protein acetylation functionality, so that it then could be selected for. (blogspot.com)
  • New evidence from Wang et al, of Brown University suggests nuclear accumulation of Survivin is achieved by acetylation at the lysine 129 position, instigated by CBP (CREB binding protein). (articlewebdirectory.com)
  • Proteome-wide analyses reveal diverse functions of protein acetylation and succinylation modifications in fast growing stolons of bermudagrass (Cynodon dactylon L. (plex.page)
  • These results partially explained the varying growth disturbances of bermudagrass stolons during treatment with sodium butyrate and sodium malonate, both of which interfere with protein acetylation and succinylation, respectively. (plex.page)
  • The results not only provide new insights into clonal growth of bermudagrass, but also provide a rich resource for functional studies of protein lysine acetylation and succinylation in plants. (plex.page)
  • Protein acetylation is conserved across phylogeny and has been recognized as one of the most prominent post-translational modifications since its discovery nearly 60 years ago. (bvsalud.org)
  • This modification, called N -acetylation, helps protect and stabilize the protein. (medlineplus.gov)
P300-mediated acetylation1
  • p300-mediated acetylation of the Rothmund-Thomson-syndrome gene product RECQL4 regulates its subcellular localization. (medscape.com)
Methylation and acetylation1
  • Conversely, methylation and acetylation of the phenolic hydroxyls, esterification of the carboxylic group of pre-cannabinoids, and introduction of a second prenyl moiety were all detrimental for antibacterial activity. (erowid.org)
Epigenetic1
  • Epigenetic markers, such as histon e acetylation and DNA methylation, determine chromatin organization. (rehabsociety.org.hk)
Transcriptional1
  • In addition, our RNA-seq analysis revealed 1330 upregulated and 1081 downregulated transcripts on Hst3p inhibition, and among them, we discovered 87 genes whose transcriptional increase was closely associated with H3K56 acetylation on their promoters, as well as some well-known regulators of phenotypic switching and virulence. (plex.page)
Slow acetylation2
  • Slow acetylation is inherited in an tertiary care referral centre drawing patients autosomal recessive fashion [ 1 ]. (who.int)
  • Association of slow acetylation profile of NAT2 with breast and gastric cancer risk in Brazil. (cdc.gov)
Lysine residues1
  • Acetylation of lysine residues neutralizes the positive charge thereby disturbing contacts with DNA or other histones. (uni-goettingen.de)
Posttranslational modification3
  • We report here that such clearance can be achieved by posttranslational modification of the mutant Huntingtin (Htt) by acetylation at lysine residue 444 (K444). (nih.gov)
  • Lysine acetylation is an important posttranslational modification involved in regulating eukaryotic gene expression and other essential processes. (edu.au)
  • ABSTRACT Lysine acetylation is a highly conserved posttranslational modification that plays essential roles in a variety of biological processes in a variety of organisms. (plex.page)
Chromatin organization2
  • Now open: Evolving insights to the roles of lysine acetylation in chromatin organization and function. (bvsalud.org)
  • In this review , we discuss mechanisms and dynamics of acetylation in chromatin organization and DNA -templated processes, including gene transcription and DNA repair and replication. (bvsalud.org)
Histones2
  • Acetylation is an histones modification, that in most of cases ( but not always, always and never does not apply to biology ) correlates with an open chromatin also reffered as euchormatin. (rehabsociety.org.hk)
  • This meeting celebrates the 50th anniversary of the paper "Acetylation and methylation of histones and their possible role in the regulation of RNA synthesis" published by V.G. Allfrey, R Faulkner and A. E. Mirsky which was published in PNAS in 1964. (bdebate.org)
Proteins6
  • Reversible lysine acetylation (RLA) is a widespread regulatory mechanism that modulates the function of proteins involved in diverse cellular processes. (mit.edu)
  • Previous laboratory results have shown an increase in acetylation of several members of the family of hnRNPs, proteins involved in pre-mRNA analyzed in CRC cells that differ in the mutational status of KRAS. (upv.es)
  • The results indicate that acetylation/deacetylation of survivin can affect the ability of STAT3 to activate tumour-causing proteins, suggesting a possible route for therapies against STAT3-dependent tumours. (articlewebdirectory.com)
  • A total of 4657 lysine acetylation sites on 1914 proteins and 226 lysine succinylation sites on 128 proteins were successfully identified by liquid chromatography and tandem mass spectrometry, respectively. (plex.page)
  • While it is known that this versatility is the result of the many actin-remodeling activities of actin-binding proteins, such as Arp2/3 and cofilin, recent work also implicates posttranslational acetylation or arginylation of the actin N terminus itself as an equally important regulatory mechanism. (jbc.org)
  • The human mind acetylome reveals that decreased acetylation of mitochondrial proteins associates with Alzheimer's illness Metabolic modifications that correlate to cognitive modifications are well-known in AD. (proteomicsresource.org)
Mammalian cells1
  • Site-specific Identification of Lysine Acetylation Stoichiometries in Mammalian Cells. (fuse.tv)
Mechanism2
  • Together, our study demonstrates that elevation of acetylation level of APE1 in tumor could be a novel mechanism by which cells handle the elevated levels of DNA damages in response to genotoxic stress and maintain sustained proliferation. (nebraska.edu)
  • Our results shed light on a new transcription-based mechanism that mediates the inflammatory effect of PAD4 and establish the interplay between citrullination and acetylation in the control of E2F-1 as a regulatory interface for driving inflammatory gene expression. (umassmed.edu)
Regulation1
  • The significance of the regulation of transcription by acetylation is properly documented. (proteomicsresource.org)
Acetyltransferases1
  • The significance of acetylation events is indicated by the associations of lysine acetyltransferases , deacetylases, and acetyl- lysine readers with developmental disorders and pathologies . (bvsalud.org)
Biology2
  • Using?my?background?in?acetylation?biology,?I?previously?demonstrated?that?acetylation?of?the? (neurodegenerationresearch.eu)
  • I will also discuss the initial characterization of a newly-generated animal model showing a possible impact of the ER-based acetylation machinery on the biology of the immune system as well as the risk for cancer. (wisc.edu)
Inhibition1
  • Conclusions: This study demonstrates that autophagy-derived acetyl-CoA promotes Snail acetylation and thereby facilitates invasion and metastasis of KRAS-LKB1 co-mutated lung cancer cells and that inhibition of the autophagy/acetyl-CoA/acetyl-Snail axis using CAMKK2 or ACLY inhibitors could be a potential therapeutic strategy to suppress metastasis of KL lung cancer. (elsevier.com)
Residues1
  • To test this, we replaced T2 with three different amino acids alanine (T2/A), glutamine (T2/Q), and arginine (T2/R). We hypothesized T2/A and T2/Q would function as acetylation-mimicking residues with similar activity to wild-type ESAT-6, while T2/R serves as acetylation negative control with less activity. (utep.edu)
20191
  • Carbonylative Acetylation of Heterocycles , Youcan Zhang, Zhiping Yin, Xiao-Feng Wu, European Journal of Organic Chemistry 2019 . (chemistryviews.org)
Isoniazid3
  • Acetylation of acetylhydrazine, the toxic metabolite of isoniazid, in humans. (aspetjournals.org)
  • In each subject (two fast and three slow acetylators) the rate of elimination of acetylhydrazine was similar to the rate of elimination of isoniazid suggesting that the two compounds are subject to the same acetylation polymorphism. (aspetjournals.org)
  • The data indicate that therapeutic concentrations of isoniazid and its metabolites inhibit the acetylation of acetylhydrazine in humans. (aspetjournals.org)
Biological1
Genes1
  • Low levels of chromatin acetylation results in less of the ECM genes aggrecan and COL2A1 which may interfere with optimal growth. (heightquest.com)
Substrates1
  • Non-acetylated H3 peptide or an H3 peptide that had been previously acetylated on K9 both serve as excellent substrates for HAG1-catalyzed acetylation. (or.jp)
Starch2
  • Effect of reaction time on the acetylation of plantain starch. (rmiq.org)
  • En: Starch: Chemistry and Technology. (rmiq.org)
Interfere1
  • None were taking bolic pathway in the biotransformation of drugs that would interfere with acetylation a number of drugs such as procainamide, nor were any on any drugs known to be hydralazine, sulphonamides, isoniazide and polymorphically N-acetylated. (who.int)
Vitro3
  • Finally, we suggest that acetylation of SlAcs depends on factors or conditions other than those present in our in vitro system. (mit.edu)
  • established?the?disease?relevance?of?TDP?43?acetylation,?the?independent?phase?will?utilize?in?vitro?and? (neurodegenerationresearch.eu)
  • Modulation of APE1 acetylation level in cells alters AP site repair capacity of the cell extracts in vitro. (nebraska.edu)
Aromatic1
  • Acetyl coenzyme A is a cofactor in the N-acetylation of many carcinogens, such as aromatic amines and alkylanilines, by a xenobiotic metabolizing enzyme. (plex.page)
Metabolite2
  • The amine functional groups may be metabolized in the liver to the acetylated and / or glucuronidated forms, or they may be oxidized to a hydroxylamine form, which undergoes further conversion via an acetylation reaction to form a N-acetoxy metabolite. (cdc.gov)
  • Current applications for this instrument include a research project into the function of terpenoids in plant immune response, the analysis of sugars in a wide variety of samples by acetylation for GC, profiling of volatile compounds in seaweed and metabolite profiling by methyl chloroformate derivatisation, by the method of Smart et al (1). (drchrispook.com)
Alzheimer's1
  • The discovery of the ER-based acetylation machinery: from aging to Alzheimer's disease … cancer too? (wisc.edu)
Cells3
  • Importantly, in the absence of APE1 acetylation, cells accumulate AP sites in the genome and show increased sensitivity to DNA damaging agents. (nebraska.edu)
  • Results: We found that autophagy in KL cancer cells increased acetyl-coenzyme A (acetyl-CoA), which facilitated the acetylation and stabilization of the EMT-inducing transcription factor Snail. (elsevier.com)
  • TFEB acetylation in KL cancer cells sustained pro-metastatic autophagy in a mammalian target of rapamycin complex 1 (mTORC1)-independent manner. (elsevier.com)
Mechanisms1
  • However, the molecular mechanisms by which acetylation and arginylation alter the properties of actin are not well understood. (jbc.org)
Molecular1
  • Conclusions: These studies show that the memory modulating ability of drugs that enhance acetylation is sensitive to a variety of behavioral and molecular conditions. (elsevier.com)
Results4
  • Results were analyzed statistically based on a complete randomized design to determine effect of the acetylation on fire resistance. (irg-wp.com)
  • Results indicated that the acetylation affects ignition and glowing in plywood. (irg-wp.com)
  • The experimental results indicated that the use of acetylation information can provide significant improvements in microarray imputation accuracy. (biomedcentral.com)
  • Our results indicate that as SOD2 expression increases acetylation of lysine 68 ensues. (uthscsa.edu)
Nuclear1
  • Shear flow was able to induce H2B acetylation and nuclear spreading by CFL2/F-actin cytoskeletal reorganization. (biomedcentral.com)
Determination1
  • None of the participants had a history of drug-induced lupus prior to Acetylation is considered a major meta- phenotype determination. (who.int)
Greatly1
  • Binding affinity between CFP-10 and ESAT-6 is greatly reduced after acetylation, suggesting that N-α-terminally acetylation could be the cause for the dissociation of the ESAT-6/CFP-10 heterodimer. (utep.edu)
Neurodegenerative1
  • Here, I will describe the biochemical properties of the individual components of the ER-based acetylation machinery and their impact on different neurodegenerative diseases, including AlzheimeraEuroTMs disease and spastic paraplegias. (wisc.edu)
Neurodegeneration1
  • In contrast, mutant Htt that is rendered resistant to acetylation dramatically accumulates and leads to neurodegeneration in cultured neurons and in mouse brain. (nih.gov)
Procedure1
  • Youcan Zhang, Zhiping Yin, and Xiao-Feng Wu, Leibniz-Institut für Katalyse e.V., University of Rostock, Germany, have developed a procedure for the carbonylative acetylation of heterocycles. (chemistryviews.org)
Subject1
Current and Emerging Therapeutics for Cutaneous T-Cell Lymphoma: Histone Deacetylase Inhibitors
Current and Emerging Therapeutics for Cutaneous T-Cell Lymphoma: Histone Deacetylase Inhibitors (hindawi.com)
Molecules | Free Full-Text | Acylated Flavone Glycosides from the Roots of Saussurea lappa and Their Antifungal Activity
Molecules | Free Full-Text | Acylated Flavone Glycosides from the Roots of Saussurea lappa and Their Antifungal Activity (mdpi.com)
Biomolecules | Free Full-Text | Targeting α-Synuclein for PD Therapeutics: A Pursuit on All Fronts
Biomolecules | Free Full-Text | Targeting α-Synuclein for PD Therapeutics: A Pursuit on All Fronts (mdpi.com)
Microtubules tune mechanosensitive cell responses | Nature Materials
Microtubules tune mechanosensitive cell responses | Nature Materials (nature.com)
Nuclear receptor corepressor and histone deacetylase 3 govern circadian metabolic physiology | Nature
Nuclear receptor corepressor and histone deacetylase 3 govern circadian metabolic physiology | Nature (nature.com)
العربية
العربية (extranet.who.int)
العربية
العربية (extranet.who.int)
Novel skin phenotypes revealed by a genome-wide mouse reverse genetic screen | Nature Communications
Novel skin phenotypes revealed by a genome-wide mouse reverse genetic screen | Nature Communications (nature.com)
Publications | National Institute on Alcohol Abuse and Alcoholism | Update on the Genetics of Alcoholism
Publications | National Institute on Alcohol Abuse and Alcoholism | Update on the Genetics of Alcoholism (pubs.niaaa.nih.gov)
Search Results
Search Results (biolegend.com)
Giant Cell Arteritis (Temporal Arteritis) Medication: Corticosteroids, Interleukin Inhibitors, Immunosuppressant Agents,...
Giant Cell Arteritis (Temporal Arteritis) Medication: Corticosteroids, Interleukin Inhibitors, Immunosuppressant Agents,... (medscape.com)
Acetylator phenotype in Iraqi patients with systemic lupus erythematosus
Acetylator phenotype in Iraqi patients with systemic lupus erythematosus (extranet.who.int)
Nutrients | Free Full-Text | Per- and Polyfluoroalkyl Substance Exposure Combined with High-Fat Diet Supports Prostate Cancer...
Nutrients | Free Full-Text | Per- and Polyfluoroalkyl Substance Exposure Combined with High-Fat Diet Supports Prostate Cancer... (mdpi.com)
Aminoacylase 1 deficiency: MedlinePlus Genetics
Aminoacylase 1 deficiency: MedlinePlus Genetics (medlineplus.gov)
ACY1 gene: MedlinePlus Genetics
ACY1 gene: MedlinePlus Genetics (medlineplus.gov)
Activation of Tel1ATM kinase requires Rad50 ATPase and long nucleosome-free DNA but no DNA ends - Journal of Biological...
Activation of Tel1ATM kinase requires Rad50 ATPase and long nucleosome-free DNA but no DNA ends - Journal of Biological... (jbc.org)
Browsing by Title
Browsing by Title (apps.who.int)
Meningitis Lab Manual: PCR Detection and Characterization | CDC
Meningitis Lab Manual: PCR Detection and Characterization | CDC (cdc.gov)
Rutin | GreenMedInfo | Substance | Natural Medicine | Alternative
Rutin | GreenMedInfo | Substance | Natural Medicine | Alternative (greenmedinfo.com)
Human genome structure | Masaryk University
Human genome structure | Masaryk University (muni.cz)
(emedicine.medscape.com)
Publications | National Institute on Alcohol Abuse and Alcoholism | Stess and Alcohol
Publications | National Institute on Alcohol Abuse and Alcoholism | Stess and Alcohol (pubs.niaaa.nih.gov)
Hansen's Disease (Leprosy): Pharmacotherapy
Hansen's Disease (Leprosy): Pharmacotherapy (medscape.com)
Hansen's Disease (Leprosy): Pharmacotherapy
Hansen's Disease (Leprosy): Pharmacotherapy (medscape.com)
Drug-Induced Lupus Erythematosus Clinical Presentation: History, Physical Examination, Complications
Drug-Induced Lupus Erythematosus Clinical Presentation: History, Physical Examination, Complications (medscape.com)
Drug-Induced Lupus Erythematosus: Background, Pathophysiology, Etiology
Drug-Induced Lupus Erythematosus: Background, Pathophysiology, Etiology (medscape.com)
Drug-Induced Lupus Erythematosus Workup: Antibody Assays, Other Tests, Tissue Analysis and Histologic Findings
Drug-Induced Lupus Erythematosus Workup: Antibody Assays, Other Tests, Tissue Analysis and Histologic Findings (medscape.com)
Drug-Induced Lupus Erythematosus Workup: Antibody Assays, Other Tests, Tissue Analysis and Histologic Findings
Drug-Induced Lupus Erythematosus Workup: Antibody Assays, Other Tests, Tissue Analysis and Histologic Findings (medscape.com)