Molecular Biology: A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.Genetic Therapy: Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.Access to Information: Individual's rights to obtain and use information collected or generated by others.Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. 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(1/93) Cytosine methylation in a CpG sequence leads to enhanced reactivity with Benzo[a]pyrene diol epoxide that correlates with a conformational change.

Benzo[a]pyrene (B[a]P) is a widespread environmental carcinogen that must be activated by cellular metabolism to a diol epoxide form (BPDE) before it reacts with DNA. It has recently been shown that BPDE preferentially modifies the guanine in methylated 5'-CpG-3' sequences in the human p53 gene, providing one explanation for why these sites are mutational hot spots. Using purified duplex oligonucleotides containing identical methylated and unmethylated CpG sequences, we show here that BPDE preferentially modified the guanine in hemimethylated or fully methylated CpG sequences, producing between 3- and 8-fold more modification at this site. Analysis of this reaction using shorter duplex oligonucleotides indicated that it was the level of the (+)-trans isomer that was specifically increased. To determine if there were conformational differences between the methylated and unmethylated B[a]P-modified DNA sequences that may be responsible for this enhanced reactivity, a native polyacrylamide gel electrophoresis analysis was carried out using DNA containing isomerically pure B[a]P-DNA adducts. These experiments showed that each adduct resulted in an altered gel mobility in duplex DNA but that only the presence of a (+)-trans isomer and a methylated C 5' to the adduct resulted in a significant gel mobility shift compared with the unmethylated case.  (+info)

(2/93) Damaged DNA-binding proteins: recognition of N-acetoxy-acetylaminofluorene-induced DNA adducts.

Proteins which bind to the DNA damaged by genotoxic agents can be detected in all living organisms. Damage-recognition proteins are thought to be generally involved in DNA repair mechanisms. On the other hand, the relevance to DNA repair of some other proteins which show elevated affinity to damaged DNA (e.g. HMG-box containing proteins or histone H1) has not been established. Using the electrophoretic mobility-shift assay we have investigated damage-recognition proteins in nuclei from rat hepatocytes. We detected two different protein complexes which preferentially bound the DNA damaged by N-acetoxy-acetylaminofluorene. One of them also recognized the DNA damaged by benzo(a)pyrene diol epoxide (yet with much lower efficiency). The proteins which bind to damaged DNA are permanently present in rat cells and their level does not change after treatment of animals with the carcinogens. Differences in the affinity of the detected damage-recognition proteins to DNA lesion evoked by either carcinogen did not correlate with more efficient removal from hepatic DNA of 2-acetylaminofluorene-induced adducts than benzo(a)pyrene-induced ones.  (+info)

(3/93) DNA-damaging effects of genotoxins in mixture: nonadditive effects of aflatoxin B1 and N-acetylaminofluorene on their mutagenicity in Salmonella typhimurium.

Most animal genotoxicity studies have used exposures to single chemicals; humans, however, are potentially exposed to mixtures of genotoxins. Cancer and developmental toxicity risks associated with genotoxins in mixture are generally estimated by assuming additivity of the components. Two or more genotoxins acting sequentially or simultaneously may present a greater or lesser hazard than that predicted by simple addition of their potencies. Previously, we studied the effect of one genotoxin on the binding of a second genotoxin to DNA in an in vitro system and demonstrated that consecutive binding of the two toxins was not additive. In the present study, the effect of one genotoxin on the mutagenicity of another was evaluated for two well-known genotoxins using the Salmonella assay. Pretreatment of frameshift strains TA98 and TA1538 with AFB1-8,9-epoxide (17.3 ng/plate) enhanced the mutagenicity induced by subsequent exposure to N-acetoxy-acetylaminofluorene (N-AcO-AAF) approximately 2-3 times above theoretical values for additivity. Pretreatment of base-substitution strain TA100 with N-AcO-AAF (0.1 microg/plate) inhibited the mutagenicity following subsequent exposure to AFB1-8,9-epoxide by 3 times below the theoretical additive value. Concentration-response relationships for these enhancing or inhibitory effects were demonstrated using increasing concentrations of the first genotoxin during pretreatment. These results demonstrate effects, other than additive, of sequential exposures to two genotoxins on the induction of mutations in a bacterial system.  (+info)

(4/93) Synthesis of nuclear proteins during DNA repair synthesis in human diploid fibroblasts damaged with ultraviolet radiation of N-acetoxy-2-acetylaminofluroene.

We have examined the accumulation of newly synthesized nuclear proteins into nuclei during DNA repair synthesis in confluent WI-38 human diploid fibroblasts damaged with ultraviolet radiation or N-acetoxy-2-acetylaminofluroene. In contrast to a marked stimulation of DNA repair synthesis, stimulation of amino acid incorporation into histone polypeptides or into the various molecular weight classes of nonhistone nuclear proteins was not observed. These results suggest that detectable stimulation of newly synthesized nuclear protein incorporation into nuclei does not accompany DNA repair synthesis induced by ultraviolet radiation or a direct acting chemical carcinogen. At least for the special case of repair, DNA synthesis may be uncoupled from histone synthesis.  (+info)

(5/93) Enhancement of postreplication repair in Chinese hamster cells.

Alkaline sedimentation profiles of pulse-labeled DNA from Chinese hamster cells showed that DNA from cells treated with N-acetoxy-acetylaminofluorene or ultraviolet radiation was made in segments smaller than those from untreated cells. Cells treated with a small dose (2.5 muM) of N-acetoxy-acetylaminofluorene or (2.5 J-m-2) 254-nm radiation, several hours before a larger dose (7-10 muM) of N-acetoxy-acetylaminofluorene or 5.0 J.m-2 of 254 nm radiation, also synthesized small DNA after the second dose. However, the rate at which this small DNA was joined together into parental size was appreciably greater than in absence of the small dose. This enhancement of postreplication repair (as a result of the initial small dose) was not observed when cells were incubated with cycloheximide between the two treatments. The results suggest that N-acetoxy-acetylaminofluorene and ultraviolet-damaged DNA from Chinese hamster cells are repaired by similar postreplicative mechanisms that require de novo protein synthesis for enhancement.  (+info)

(6/93) DNA-protein cross-linking by chemical carcinogens in mammalian cells.

The induction of DNA cross-linking in mammalian cells by various carcinogens was investigated by the method of alkaline elution. A dose-dependent increase in DNA cross-linking was seen following exposure of human fibroblasts to N-acetyoxy-2-acetylaminofluorene and following exposure of mouse embryo cells to 7,12-dimethylbenz[a]-anthracene. No cross-link effect was seen following treatment with N-methyl-N'-nitro-N-nitrosoguanidine, benz-[a]anthracene, benz[A]anthracene-5,6-dihydroepoxide, or metabolic inhibitors. The cross-linking appeared to be DNA-protein in nature since proteinase treatment removed the effect. DNA single-strand breaks were also induced by several of these agents in the case of N-acetoxy-2-acetylaminofluorene and N-methyl-N'-nitro-N-nitrosoguanidine, approximately 70 to 90% of these breaks were rejoined after an 18-hr incubation in fresh medium, whereas repair of the cross-links induced by N-acetoxy-2-acetylaminofluorene was slight at this time.  (+info)

(7/93) Diverse chemical carcinogens fail to induce G(1) arrest in MCF-7 cells.

The effect of three reactive potent chemical carcinogens on the passage of MCF-7 cells through the cell cycle was investigated. While these cells, which express wild-type p53, were arrested in G(1) after treatment with actinomycin D (a positive control), treatment with anti-benzo[a]pyrene dihydrodiol epoxide, N-acetoxy-N-2-fluorenylacetamide or N-methyl-N'-nitro-N-nitrosoguanidine, at doses consistent with survival of significant numbers of cells, caused the cells to accumulate in S phase, with little increase in those in G(1). This property of these three reactive potent carcinogens, of diverse chemical types, to induce evasion of G(1) arrest (the stealth property) presumably increases the likelihood of malignant change, because DNA replication continues on a damaged template. This stealth characteristic may be a major contributor to the tumorigenicity of DNA-adducting chemical carcinogens in general.  (+info)

(8/93) Overlapping pathways for repair of damage from ultraviolet light and chemical carcinogens in human fibroblasts.

DNA excision repair was measured in cultured human fibroblasts after single or dual treatments with ultraviolet radiation, 4-nitroquinoline 1-oxide, or N-acetoxy-2-acetylaminofluorene. Three approaches were used to monitor repair: unscheduled DNA synthesis, measured by autoradiography; repair replication, measured by the incorporation of a density-labeled DNA precursor into repaired regions; and excision of ultraviolet endonuclease-sensitive sites. When a single repair- saturating dose of one of the three carcinogens was administered, little stimulation of unscheduled DNA synthesis or repair replication could be observed by additional treatment with one of the other carcinogens. In no instance was total additivity of repair observed. These observations were confirmed by showing that the excision of endonuclease-sensitive sites produced by ultraviolet damage (i.e., pyrimidine dimers) was inhibited by exposure to 4-nitroquinoline 1-oxide and N-acetoxy-2-acetylaminofluorene. The data indicate that the repair of lesions induced by these substances may have common rate-limiting steps, a conclusion previously indicated by the repair deficiency in xeroderma pigmentosum cells in which a single mutation eliminates the repair of damage caused by each of these agents.  (+info)

*  Acetoxyacetylaminofluorene
... is a derivative of 2-acetylaminofluorene used as a biochemical tool in the study of carcinogenesis. ...
*  Hydroxyacetylaminofluorene
Acetoxyacetylaminofluorene. ...
*  2-Acetylaminofluorene
Acetoxyacetylaminofluorene Hydroxyacetylaminofluorene "NIOSH Pocket Guide to Chemical Hazards". Centers for Disease Control and ...
*  List of MeSH codes (D02)
... acetoxyacetylaminofluorene MeSH D02.241.081.038.108.080.400 --- hydroxyacetylaminofluorene MeSH D02.241.081.038.108.189 --- ... acetoxyacetylaminofluorene MeSH D02.065.064.150.400 --- hydroxyacetylaminofluorene MeSH D02.065.064.189 --- ...
*  List of MeSH codes (D04)
... acetoxyacetylaminofluorene MeSH D04.615.389.050.400 --- hydroxyacetylaminofluorene MeSH D04.615.389.850 --- tilorone MeSH ...
Acetoxyacetylaminofluorene - Wikipedia  Acetoxyacetylaminofluorene - Wikipedia
Acetoxyacetylaminofluorene is a derivative of 2-acetylaminofluorene used as a biochemical tool in the study of carcinogenesis. ...
more infohttps://en.wikipedia.org/wiki/Acetoxyacetylaminofluorene
Acyclic Acids (Ethanoic Acids)  < Carboxylic Acids  << Organic Compounds (Organic Chemicals)  <<< Compounds, Elements & more  @...  Acyclic Acids (Ethanoic Acids) < Carboxylic Acids << Organic Compounds (Organic Chemicals) <<< Compounds, Elements & more @...
Acetoxyacetylaminofluorene *Hydroxyacetylaminofluorene *Allylisopropylacetamide *Iodoacetamide *Linezolid *Piracetam * ...
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1 - Binary Option Augustow  1 - Binary Option Augustow
2-acetoxyacetylaminofluorene; 2-AAF, 2-acetyl aminofluorene; 2-AN, 2-aminoanthracene; 4-NQO, 4-nitroquinoline-N-oxide; 6TG, 6- ...
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Plus it  Plus it
Transformed cell foci were induced in hamster secondary cells after treatment for 6 days with acetoxyacetylaminofluorene, ...
more infohttp://cancerres.aacrjournals.org/content/37/10/3508
Volume 4 -  GTMB: Gene Therapy & Molecular Biology  Volume 4 - GTMB: Gene Therapy & Molecular Biology
A specific 36-bp DNA sequence was either UV-irradiated or damaged by benzo(a)pyrene diol epoxide and N- acetoxy-acetylaminofluorene ...
more infohttp://gtmb.org/?vol=4
Category:Organic compound stubs - Wikipedia  Category:Organic compound stubs - Wikipedia
Acetoxyacetylaminofluorene. *6-Acetoxydihydrotheaspirane. *Aceturic acid. *Acetyl bromide. *N-Acetylglutamic acid. *N- ...
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HMGB1/2 can target DNA for illegitimate cleavage by the RAG1/2 complex | BMC Molecular Biology | Full Text  HMGB1/2 can target DNA for illegitimate cleavage by the RAG1/2 complex | BMC Molecular Biology | Full Text
... pyrene diol epoxide and N-acetoxy-acetylaminofluorene. Cancer Lett. 2000, 158 (1): 17-25. 10.1016/S0304-3835(00)00517-6View ...
more infohttps://bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-10-24
dict.md | A  dict.md | A
Acetoxyacetylaminofluorene [Chemical/Ingredient]. acetoxyacrylic acid ethyl ester. acetoxybutynylbithiophene deacetylase ...
more infohttp://en.dict.md/A/60
Search Articles | University of Toronto Libraries  Search Articles | University of Toronto Libraries
Acetoxyacetylaminofluorene - pharmacology , Phosphorylation - physiology , Protein Kinases - metabolism , Protein Kinases - ...
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Words with LAM :: FindTheWord.info -- Crossword Help - Crossword Solver - Scrabble Cheat and Wordfeud Cheat - Crossword...  Words with LAM :: FindTheWord.info -- Crossword Help - Crossword Solver - Scrabble Cheat and Wordfeud Cheat - Crossword...
Show more information about ACETOXYACETYLAMINOFLUORENE. Search for acetoxyacetylaminofluorene in: Wikipedia Wiktionary Google ...
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Utilizador:Tribuno da Plebe/oficina - Wikcionário  Utilizador:Tribuno da Plebe/oficina - Wikcionário
... acetosity acetosulfone acetosyringone acetotartrate acetous acid acetowhitening acetoxolone acetoxy acetoxyacetylaminofluorene ...
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Cellular biology synonyms, cellular biology antonyms - FreeThesaurus.com  Cellular biology synonyms, cellular biology antonyms - FreeThesaurus.com
Synonyms for cellular biology in Free Thesaurus. Antonyms for cellular biology. 6 words related to cytology: stainability, biological science, biology, microscopic anatomy, cytogenetics, fix. What are synonyms for cellular biology?
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Download System Modeling In Cellular Biology From Concepts To Nuts And Bolts  Download System Modeling In Cellular Biology From Concepts To Nuts And Bolts
Deol download system modeling in cellular biology, Evans JR, Dhahbi J, et al. therapy research ensues more Used and lifestyle-induced than ancient tree and urination in column: heavy use for the cancer. Alberti KG, Eckel RH, Grundy SM, et al. regarding the bioactive blood: a White sick intake of the International Diabetes Federation Task Force on Epidemiology and Prevention; National Heart, Lung, and Blood Institute; American Heart Association; World Heart Federation; International Atherosclerosis Society; and International Association for the weighting of Obesity. Fernandez-Arroyo S, Gomez-Martinez A, Rocamora-Reverte L, et al. download system modeling in cellular biology from concepts to nuts and of factors for the 8am cripple of various parsnips from fluid alternative LOUIS in treated aloe rats. Gronbaek M, Becker U, Johansen D, et al. link of Breast understood and stage from all birds, incessant mouse growth, and browser. The ovarian simple download system modeling in cellular biology from ...
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Molecular & Cellular Biology | UA Outcomes Assessment  Molecular & Cellular Biology | UA Outcomes Assessment
The Department of Molecular and Cellular Biology: Our mission is to reveal the secrets of nature and to educate the next generation of science innovators.. Research. We use innovative tools in our research to reveal fundamental biological processes that are common to all life on Earth. Our faculty focus on model systems that allow us to query the most basic of questions about nature, whether they be at the molecular level, at the level of a cell or organism or in development of clinical application of new human disease therapies. We have a diverse group of research interests including cancer, neurobiology, heart development and disease, plant development, evolutionary biology, cell signaling, gene expression, RNA biology, genetic networks and systems biology, and genome stability. For more details, we encourage you to browse our faculty research websites.. Education. We offer student-centered educational experiences for undergraduate and graduate students. The Molecular and Cellular Biology ...
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LGC publishes new guide on practical laboratory skills for molecular biologists - African Orphan Crops Consortium  LGC publishes new guide on practical laboratory skills for molecular biologists - African Orphan Crops Consortium
This new guide is intended to support those working in a molecular biology laboratory.. Tim Wilkes, researcher in LGC's Molecular Biology team, and co-author of the guide, explains, "An analyst working in any kind of laboratory will require a basic set of practical skills, but molecular biology laboratories provide scenarios and analytical materials which demand specific practical considerations.". This new best practice guide covers the skills and other measures required to produce valid results in a molecular biology laboratory − results that are not compromised by poor practical technique, possible cross-contamination or non-validated methodology.. There are sections on basic practices, such as measuring mass or volume, and preparing solutions of known concentration. Additional skills that relate to the nature of the materials being handled in a molecular biology laboratory, such as preventing contamination of the samples and protecting the analyst, critical concerns for molecular ...
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EMBL Heidelberg - The European Molecular Biology Laboratory  EMBL Heidelberg - The European Molecular Biology Laboratory
The European Molecular Biology Laboratory (EMBL) is Europe's flagship laboratory for basic research in molecular biology. EMBL operates from five sites: the main laboratory in Heidelberg, and Outstations in Hinxton (EBI), Grenoble, Hamburg, and Monterotondo near Rome.
more infohttps://www.embl.de/index.php
EMBL Hamburg - The European Molecular Biology Laboratory  EMBL Hamburg - The European Molecular Biology Laboratory
The European Molecular Biology Laboratory (EMBL) is Europe's flagship laboratory for basic research in molecular biology. EMBL operates from five sites: the main laboratory in Heidelberg, and Outstations in Hinxton (EBI), Grenoble, Hamburg, and Monterotondo near Rome.
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BIO-410 Molecular Biology Techniques I (4.00)  BIO-410 Molecular Biology Techniques I (4.00)
... Introduces modern molecular biology techniques utilizing nucleic acids (DNA and RNA). Includes nucleic acid purification, quantitation, cloning and restriction enzyme digests. Advanced techniques include Southern and Northern analysis, polymerase chain reaction (PCR), real-time PCR and DNA sequencing. Stresses proficiency in techniques and proper analysis of results. Lab included. Credits: 4, Hours: (1/6/0/0), Arts & Sciences Elective Code: B. ...
more infohttp://www.kirkwood.edu/catalog/current/bio-410-molecular-biology-techniques-i-400.htm
Computational Biologist (Mathematical Modelling) - European Molecular Biology Laboratory (EMBL) - jobs.ac.uk  Computational Biologist (Mathematical Modelling) - European Molecular Biology Laboratory (EMBL) - jobs.ac.uk
Sorry, but the advert you were looking for has expired. To see more employment opportunities with European Molecular Biology Laboratory (EMBL), please click here. Perhaps you were looking for something totally different? In that case, it might be best start a new job search from our Home Page. You might also find our Site Map useful too. ...
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Postdoctoral Fellow - Mass Spectrometry and Structural Proteomics - European Molecular Biology Laboratory (EMBL) - jobs.ac.uk  Postdoctoral Fellow - Mass Spectrometry and Structural Proteomics - European Molecular Biology Laboratory (EMBL) - jobs.ac.uk
Sorry, but the advert you were looking for has expired. To see more employment opportunities with European Molecular Biology Laboratory (EMBL), please click here. Perhaps you were looking for something totally different? In that case, it might be best start a new job search from our Home Page. You might also find our Site Map useful too. ...
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Laboratory Animal Technician - European Molecular Biology Laboratory (EMBL) - jobs.ac.uk  Laboratory Animal Technician - European Molecular Biology Laboratory (EMBL) - jobs.ac.uk
Sorry, but the advert you were looking for has expired. To see more employment opportunities with European Molecular Biology Laboratory (EMBL), please click here. Perhaps you were looking for something totally different? In that case, it might be best start a new job search from our Home Page. You might also find our Site Map useful too. ...
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Treasury Accountant - European Molecular Biology Laboratory (EMBL) - jobs.ac.uk  Treasury Accountant - European Molecular Biology Laboratory (EMBL) - jobs.ac.uk
Sorry, but the advert you were looking for has expired. To see more employment opportunities with European Molecular Biology Laboratory (EMBL), please click here. Perhaps you were looking for something totally different? In that case, it might be best start a new job search from our Home Page. You might also find our Site Map useful too. ...
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Leica EM Cryo CLEM - Publications: Leica Microsystems  Leica EM Cryo CLEM - Publications: Leica Microsystems
New hardware and workflows for semi-automated correlative cryo-fluorescence and cryo-electron microscopy tomography, Martin Schorba, b, Leander Gaechterc, Ori Avinoama, Frank Sieckmannd, Mairi Clarkea, Cecilia Bebeacuaa, e, Yury S. Bykova, Andreas F.-P. Sonnena, f, Reinhard Lihlg, John A.G. Briggsa, e, f,, a Structural and Computational Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany, b Electron Microscopy Core Facility, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany, c Leica Microsystems (Schweiz) AG, Max Schmidheiny-Strasse 201, 9435 Heerbrugg, Switzerland, d Leica Microsystems GmbH, Am Friedensplatz 3, 68165 Mannheim, Germany, e Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany, f Molecular Medicine Partnership Unit, EMBL/Universitätsklinikum Heidelberg, Heidelberg, Germany, g Leica Mikrosysteme GmbH, Hernalser Hauptstraße 219, 1170 ...
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