Evidence that hsp90 is involved in the altered interactions of Acanthamoeba castellanii variants with bacteria. (1/132)

There are many similarities between the interactions of environmental protozoa with pathogenic bacterial species and those observed in mammalian macrophages. Since single-celled protozoa predate mammalian hosts, it is likely that interactions in environmental biofilms have selected for many of the bacterial virulence mechanisms responsible for human disease. In order to better understand bacterial-phagocyte interactions, we developed a selection for Acanthamoeba castellanii variants that are more resistant to killing by bacterial pathogens. We identified four amoebal clones that display decreased phagocytosis of bacteria but no difference in uptake of latex beads compared to wild-type amoebae. These amoebal variants display differences in cellular morphology, partial resistance to killing by bacteria, more bactericidal activity, and higher frequencies of lysosome fusion with the bacterial vacuole. Three proteins are present at lower levels in these variants than in wild-type amoebae, and matrix-assisted laser desorption ionization-time of flight mass spectrometry allowed identification of two of them as actin and hsp90. We found that specific inhibitors of hsp90 produce a similar phenotypic effect in macrophages. These data suggest that hsp90 plays a role in phagocytic and, possibly, bactericidal pathways that affect interactions of phagocytic cells with bacteria.  (+info)

The contribution of uncoupling protein and ATP synthase to state 3 respiration in Acanthamoeba castellanii mitochondria. (2/132)

Mitochondria of the amoeba Acanthamoeba castellanii possess a free fatty acid-activated uncoupling protein (AcUCP) that mediates proton re-uptake driven by the mitochondrial proton electrochemical gradient. We show that AcUCP activity diverts energy from ATP synthesis during state 3 mitochondrial respiration in a fatty acid-dependent way. The efficiency of AcUCP in mitochondrial uncoupling increases when the state 3 respiratory rate decreases as the AcUCP contribution is constant at a given linoleic acid concentration while the ATP synthase contribution decreases with respiratory rate. Respiration sustained by this energy-dissipating process remains constant at a given linoleic acid concentration until more than 60% inhibition of state 3 respiration by n-butyl malonate is achieved. The present study supports the validity of the ADP/O method to determine the actual contributions of AcUCP (activated with various linoleic acid concentrations) and ATP synthase in state 3 respiration of A.castellanii mitochondria fully depleted of free fatty acid-activated and describes how the two contributions vary when the rate of succinate dehydrogenase is decreased by succinate uptake limitation.  (+info)

Benzodiazepine binding to mitochondrial membranes of the amoeba Acanthamoeba castellanii and the yeast Saccharomyces cerevisiae. (3/132)

Benzodiazepine binding sites were studied in mitochondria of unicellular eukaryotes, the amoeba Acathamoeba castellanii and the yeast Saccharomyces cerevisiae, and also in rat liver mitochondria as a control. For that purpose we applied Ro5-4864, a well-known ligand of the mitochondrial benzodiazepine receptor (MBR) present in mammalian mitochondria. The levels of specific [(3)H]Ro5-4864 binding, the dissociation constant (K(D)) and the number of [(3)H]Ro5-4864 binding sites (B(max)) determined for fractions of the studied mitochondria indicate the presence of specific [(3)H]Ro5-4864 binding sites in the outer membrane of yeast and amoeba mitochondria as well as in yeast mitoplasts. Thus, A. castellanii and S. cerevisiae mitochondria, like rat liver mitochondria, contain proteins able to bind specifically [(3)H]Ro5-4864. Labeling of amoeba, yeast and rat liver mitochondria with [(3)H]Ro5-4864 revealed proteins identified as the voltage dependent anion selective channel (VDAC) in the outer membrane and adenine nucleotide translocase (ANT) in the inner membrane. Therefore, the specific MBR ligand binding is not confined only to mammalian mitochondria and is more widespread within the eukaryotic world. However, it can not be excluded that MBR ligand binding sites are exploited efficiently only by higher multicellular eukaryotes. Nevertheless, the MBR ligand binding sites in mitochondria of lower eukaryotes can be applied as useful models in studies on mammalian MBR.  (+info)

The amoebae plate test implicates a paralogue of lpxB in the interaction of Legionella pneumophila with Acanthamoeba castellanii. (4/132)

Legionella pneumophila is a bacterial parasite of freshwater amoebae which also grows in alveolar macrophages and thus causes the potentially fatal pneumonia Legionnaires' disease. Intracellular growth within amoebae and macrophages is mechanistically similar and requires the Icm/Dot type IV secretion system. This paper reports the development of an assay, the amoebae plate test (APT), to analyse growth of L. pneumophila wild-type and icm/dot mutant strains spotted on agar plates in the presence of Acanthamoeba castellanii. In the APT, wild-type L. pneumophila formed robust colonies even at high dilutions, icmT, -R, -P or dotB mutants failed to grow, and icmS or -G mutants were partially growth defective. The icmS or icmG mutant strains were used to screen an L. pneumophila chromosomal library for genes that suppress the growth defect in the presence of the amoebae. An icmS suppressor plasmid was isolated that harboured the icmS and flanking icm genes, indicating that this plasmid complements the intracellular growth defect of the mutant. In contrast, different icmG suppressor plasmids rendered the icmG mutant more cytotoxic for A. castellanii without enhancing intracellular multiplication in amoebae or RAW264.7 macrophages. Deletion of individual genes in the suppressor plasmids inserts identified lcs (Legionella cytotoxic suppressor) -A, -B, -C and -D as being required for enhanced cytotoxicity of an icmG mutant strain. The corresponding proteins show sequence similarity to hydrolases, NlpD-related metalloproteases, lipid A disaccharide synthases and ABC transporters, respectively. Overexpression of LcsC, a putative paralogue of the lipid A disaccharide synthase LpxB, increased cytotoxicity of an icmG mutant but not that of other icm/dot or rpoS mutant strains against A. castellanii. Based on sequence comparison and chromosomal location, lcsB and lcsC probably encode enzymes involved in cell wall maintenance and peptidoglycan metabolism. The APT established here may prove useful to identify other bacterial factors relevant for interactions with amoeba hosts.  (+info)

Development of colorimetric microtiter plate assay for assessment of antimicrobials against Acanthamoeba. (5/132)

We have developed and optimized a 96-well microtiter plate assay, based on the reduction of alamarBlue, to assess the efficacies of much needed new antimicrobials against Acanthamoeba species. This assay has been optimized for determination of drug efficacy against two potentially pathogenic species, Acanthamoeba castellanii and Acanthamoeba polyphaga, and has been validated by comparison of their relative susceptibilities to chlorhexidine, a drug widely used to treat Acanthamoeba keratitis. The results demonstrate that the assay is comparable to a manual counting assay and that A. polyphaga is more resistant to chlorhexidine than A. castellanii. Thus, by use of the manual counting assay, 3.125 microM chlorohexidine was almost completely effective against A. castellanii, whereas this concentration was less than 20% effective against A. polyphaga. Similar results were obtained by the alamarBlue assay. The new assay was used to determine the relative susceptibilities of A. castellanii and A. polyphaga to the alkylphosphocholines (APCs) hexadecylphosphocholine (hexadecyl-PC; miltefosine) and octadecylphosphocholine (octadecyl-PC) as well as an alkylgycerolphosphocholine, edelfosine. Both APCs studied were equally effective against A. castellanii, but octadecyl-PC was less effective than hexadecyl-PC against A. polyphaga. Both APCs were more effective than edelfosine against both Acanthamoeba species. A. polyphaga was found to be significantly less susceptible to each of the phosphocholine analogues. The newly described assay offers a number of advantages over those described previously. It is less labor-intensive than previously described assays and is sensitive and rapid, and the results can be read in a nonsubjective manner. As it is based on a standard 96-well, microtiter plate, it is amenable to automation and high throughput.  (+info)

Stable transfection of Acanthamoeba castellanii. (6/132)

A simple method for stable transfection of Acanthamoeba castellanii using plasmids which confer resistance to neomycin G418 is described. Expression of neomycin phosphotransferase is driven by the Acanthamoeba TBP gene promoter, and can be monitored by cell growth in the presence of neomycin G418 or by Western blot analysis. Transfected cells can be passaged in the same manner as control cells and can be induced to differentiate into cysts, in which form they maintain resistance to neomycin G418 for at least several weeks, although expression of neomycin phosphotransferase is repressed during encystment. Expression of EGFP or an HA-tagged EGFP-TBP fusion can be driven from the same plasmid, using an additional copy of the Acanthamoeba TBP gene promoter or a deletion mutant. The TBP-EGFP fusion is localized to the nucleus, except in a small proportion of presumptive pre-mitotic cells. EGFP expression can also be driven by the cyst-specific CSP21 gene promoter, which is completely repressed in growing cells but strongly induced in differentiating cells. Transfected cells maintain their phenotype for several weeks, even in the absence of neomycin G418, suggesting that transfected genes are stably integrated within the genome. These results demonstrate the utility of the neomycin resistance based plasmids for stable transfection of Acanthamoeba, and may assist a number of investigations.  (+info)

Structural implications of novel diversity in eucaryal RNase P RNA. (7/132)

Previous eucaryotic RNase P RNA secondary structural models have been based on limited diversity, representing only two of the approximately 30 phylogenetic kingdoms of the domain Eucarya. To elucidate a more generally applicable structure, we used biochemical, bioinformatic, and molecular approaches to obtain RNase P RNA sequences from diverse organisms including representatives of six additional kingdoms of eucaryotes. Novel sequences were from acanthamoeba (Acathamoeba castellanii, Balamuthia mandrillaris, Filamoeba nolandi), animals (Caenorhabditis elegans, Drosophila melanogaster), alveolates (Theileria annulata, Babesia bovis), conosids (Dictyostelium discoideum, Physarum polycephalum), trichomonads (Trichomonas vaginalis), microsporidia (Encephalitozoon cuniculi), and diplomonads (Giardia intestinalis). An improved alignment of eucaryal RNase P RNA sequences was assembled and used for statistical and comparative structural analysis. The analysis identifies a conserved core structure of eucaryal RNase P RNA that has been maintained throughout evolution and indicates that covariation in size occurs between some structural elements of the RNA. Eucaryal RNase P RNA contains regions of highly variable length and structure reminiscent of expansion segments found in rRNA. The eucaryal RNA has been remodeled through evolution as a simplified version of the structure found in bacterial and archaeal RNase P RNAs.  (+info)

Acanthamoeba castellanii induces host cell death via a phosphatidylinositol 3-kinase-dependent mechanism. (8/132)

Granulomatous amoebic encephalitis due to Acanthamoeba castellanii is a serious human infection with fatal consequences, but it is not clear how the circulating amoebae interact with the blood-brain barrier and transmigrate into the central nervous system. We studied the effects of an Acanthamoeba encephalitis isolate belonging to the T1 genotype on human brain microvascular endothelial cells, which constitute the blood-brain barrier. Using an apoptosis-specific enzyme-linked immunosorbent assay, we showed that Acanthamoeba induces programmed cell death in brain microvascular endothelial cells. Next, we observed that Acanthamoeba specifically activates phosphatidylinositol 3-kinase. Acanthamoeba-mediated brain endothelial cell death was abolished using LY294002, a phosphatidylinositol 3-kinase inhibitor. These results were further confirmed using brain microvascular endothelial cells expressing dominant negative forms of phosphatidylinositol 3-kinase. This is the first demonstration that Acanthamoeba-mediated brain microvascular endothelial cell death is dependent on phosphatidylinositol 3-kinase.  (+info)

Effect of growth at low temperature on the uncoupling protein activity and expression in Acanthamoeba castellanii mitochondria ...
Tytuł projektu: Rozbudowa i przekształcenie bibliograficznej bazy danych AGRO w bazę bibliograficzno-abstraktową z wykorzystaniem oprogramowania YADDA. Nr umowy: POIG 02.03.02-00-031/09 (okres realizacji 2009-2013 ...
http://agro.icm.edu.pl/agro/element/bwmeta1.element.agro-00b1a320-e7fd-4c72-9882-0361848ce821?printView=true
By releasing ADP, Acanthamoeba castellanii causes an increase in the cytosolic free calcium concentration and apoptosis in wish...
The role played by soluble molecules that may participate in acanthamoebal cytopathogenicity has yet to be fully characterized. We demonstrate here that Acanthamoeba castellanii trophozoites constitutively release ADP in the medium. Cell-free supernatants prepared from A. castellanii, by interaction with specific P2y2 purinoceptors expressed on the Wish cell membrane, caused a biphasic rise in [Ca2+]i, extensive cell membrane blebbing, cytoskeletal disorganization, and the breakdown of nuclei. Cell damage induced by amoebic supernatants was blocked by the P2y2 inhibitor Suramin. The same results were found in Wish cells exposed to purified ADP. These findings suggest that pathogenic free-living A. castellanii may have a cytopathic effect on human epithelial cells through ADP release, by a process that begins with a rise of cytosolic free-calcium concentration, and culminates in apoptosis. ...
http://eprints.uniss.it/7896/
The interaction of F-actin with phosphorylated and unphosphorylated myosins IA and IB from Acanthamoeba castellanii.<...
TY - JOUR. T1 - The interaction of F-actin with phosphorylated and unphosphorylated myosins IA and IB from Acanthamoeba castellanii.. AU - Albanesi, J. P.. AU - Hammer, J. A.. AU - Korn, E. D.. PY - 1983/8/25. Y1 - 1983/8/25. N2 - Myosins IA and IB from Acanthamoeba castellanii are single-headed molecules which, upon phosphorylation of their heavy chains by a specific kinase, express actin-activated Mg2+-ATPase activity. These myosins show no tendency to self-associate under assay conditions, a property which allows unambiguous kinetic and actin-binding data to be obtained. Both myosin isoenzymes exhibit a complex dependence of actomyosin ATPase activity on F-actin concentration. A conventional hyperbolic dependence is observed at low concentrations of F-actin but at higher F-actin concentrations, inhibition and then apparent reactivation are seen to occur. From those early portions of the velocity profiles which do not deviate from simple Michaelis-Menten type kinetics, values for the Vmax (10 ...
https://utsouthwestern.pure.elsevier.com/en/publications/the-interaction-of-f-actin-with-phosphorylated-and-unphosphorylat
Infection of Acanthamoeba castellanii by Chlamydia pneumoniae. | Applied and Environmental MicrobiologyInfection of Acanthamoeba castellanii by Chlamydia pneumoniae. | Applied and Environmental Microbiology
Chlamydia pneumoniae is an intracellular respiratory pathogen, which, similar to Legionella, might have developed mechanisms to escape the intracellular bactericidal activity of both human host cells and amoeba. We therefore investigated the intracellular growth and survival of C. pneumoniae in Acanthamoeba castellanii by using cell culture, immunofluorescence microscopy, and electron microscopy. A castellanii was incubated with purified elementary bodies of C. pneumoniae TW 183 at a concentration of 10(6) inclusion-forming units (IFU)/ml to give a ratio of approximately 1 IFU of C. pneumoniae per amoeba. Quantitative determination of chlamydial growth within A. castellanii revealed viable and infective C. pneumoniae in the range of 10(4) to 10(5) IFU/ml between days 7 and 14 postinfection. Immunofluorescence analysis and transmission electron microscopy with subsequent immunogold staining confirmed evidence of infection of the amoebae by C. Pneumoniae and additionally revealed that C. ...
https://aem.asm.org/content/63/4/1396?ijkey=61fc77adb4f701813f607a63ef5e5dbd68de3486&keytype2=tf_ipsecsha
Acanthamoeba castellanii: a new high-throughput method for drug screening in vitro - Nottingham ePrintsAcanthamoeba castellanii: a new high-throughput method for drug screening in vitro - Nottingham ePrints
Despite significant public health impact, there is no specific antiprotozoal therapy for prevention and treatment of Acanthamoeba castellanii infection. There is a need for new and efficient anti-Acanthamoeba drugs that are less toxic and can reduce treatment duration and frequency of administration. In this context a new, rapid and sensitive assay is required for high-throughput activity testing and screening of new therapeutic compounds. A colorimetric assay based on sulforhodamine B (SRB) staining has been developed for anti-Acanthamoeba drug susceptibility testing and adapted to a 96-well microtiter plate format. Under these conditions chlorhexidine was tested to validate the assay using two clinical strains of A. castellanii (Neff strain, T4 genotype [IC50 4.68±0.6 _M] and T3 genotype [IC50 5.69±0.9 _M]). These results were in good agreement with those obtained by the conventional Alamar Blue assay, OCR cytotoxicity assay and manual cell counting method. Our new assay offers an ...
http://eprints.nottingham.ac.uk/44052/
Rapid induction of microsomal delta 12(omega 6)-desaturase activity in chilled Acanthamoeba castellanii. -ORCARapid induction of microsomal delta 12(omega 6)-desaturase activity in chilled Acanthamoeba castellanii. -ORCA
The activity of microsomal delta 12-desaturase in Acanthamoeba castellanii was increased after growing cultures were chilled from the optimal growth temperature (30 degrees C) to 15 degrees C. This increase was detectable in microsomes isolated from organisms subjected to only 10 min chilling. The mechanism of induction was investigated. The increase in activity on chilling was greatly reduced when protein synthesis was blocked before the temperature shift. Thus the major mechanism for the induction of delta 12-desaturase is increased protein synthesis. delta 12-Desaturase activity was higher when assayed at 20 degrees C than when assayed at 30 degrees C, but these changes were not due to the increased solubility of O2 at 20 degrees C. The major substrate of delta 12-desaturase was found to be 1-acyl-2-oleoyl phosphatidylcholine.. ...
https://orca.cf.ac.uk/127875/
Genetic basis of survival of Mycobacterium bovis inside Acanthamoeba castellanii. - Surrey Research Insight Open AccessGenetic basis of survival of Mycobacterium bovis inside Acanthamoeba castellanii. - Surrey Research Insight Open Access
Tuberculosis remains a major threat to human health accounting for 2 million annual deaths worldwide. M. bovis causes TB in cattle which is a serious issue in the UK. Mycobacteria are widely distributed in the environments that are also colonized by free living amoebae. In the present study, free-living amoeba (A. castellanii) has been used to study the genetic factors required for the intracellular survival of M. bovis. Role of region of difference 1 (RD1), isocitrate lyase (Rv0467), ClgR (Rv2745) and the VapC (Rv2548) toxin-antitoxin system was examined for survival in amoebae. While the role of RD1 in mycobacterial survival in amoebae could not be observed, isocitrate lyase and a transcriptional regulator (ClgR) might play some part in survival of M. bovis in A. castellanii. It was found that although the mycobacteria were able to remain inside the amoebae vacuoles, they were unable to control the pH as the vacuoles remained acidic. This is very interesting as it is in contrast to macrophages ...
http://epubs.surrey.ac.uk/843112/
Genome of Acanthamoeba castellanii highlights extensive lateral gene transfer and early evolution of tyrosine kinase signaling ...Genome of Acanthamoeba castellanii highlights extensive lateral gene transfer and early evolution of tyrosine kinase signaling ...
Ac encodes 15,455 compact intron-rich genes, a significant number of which are predicted to have arisen through inter-kingdom lateral gene transfer (LGT). A majority of the LGT candidates have undergone a substantial degree of intronization and Ac appears to have incorporated them into established transcriptional programs. Ac manifests a complex signaling and cell communication repertoire, including a complete tyrosine kinase signaling toolkit and a comparable diversity of predicted extracellular receptors to that found in the facultatively multicellular dictyostelids. An important environmental host of a diverse range of bacteria and viruses, Ac utilizes a diverse repertoire of predicted pattern recognition receptors, many with predicted orthologous functions in the innate immune systems of higher organisms.. ...
http://eprints.hud.ac.uk/id/eprint/28247/
The effect of different environmental conditions on the encystation of by Yousuf Aqeel, Ruqaiyyah Siddiqui et al.The effect of different environmental conditions on the encystation of by Yousuf Aqeel, Ruqaiyyah Siddiqui et al.
In this study, Acanthamoeba castellanii was cultivated under different stress conditions to induce possible encystation. The morphological and histological properties were analysed by light and electron microscopy as well as cyst-specific staining. The findings revealed that cysts prepared through liquid medium using higher osmolarity as a trigger (10% glucose with 50mM magnesium chloride for 72 h) are similar to cysts prepared using non-nutrient agar (nutrient deprivation as a trigger in plating assays for 14 days), as determined by SDS-resistance, cyst-specific Calcofluor white staining and transmission electron microscopy. Using liquid medium assay, A. castellanii encystation was studied by exposing trophozoites to media lacking growth ingredients (phosphate buffered saline or distilled water), inappropriate temperatures (4-45°C), pH (3-9), artificial light-dark cycles, 5% CO2, and microaerophilic conditions. Optimal encystation was observed when cells were incubated in PBS with 50mM MgCl2 and 10%
https://ecommons.aku.edu/pakistan_fhs_mc_bbs/163/
ASMscience | Selection of Signature-T
Legionella pneumophila is a facultative intracellular bacillus that causes nosocomial and community-acquired pneumonia and, rarely, extrapulmonary infections in humans. Signature-tagged mutagenesis employs uniquely tagged transposons that are used to randomly mutagenize a bacterial chromosome and create a library. A library of 700 mutant clones created by signature-tagged mutagenesis was screened using this negative-selection strategy in Acanthamoeba castellanii, a free-living amoeba that may serve as an environmental reservoir of legionellae. The efficiency of invasion was studied by incubating L. pneumophila strains grown to postexponential phase with A. castellanii, using gentamicin to kill extracellular organisms and then determining remaining intracellular CFU. The behavior of the mutants was also examined in human macrophages derived from peripheral blood mononuclear cells (PBMCs) and in phorbol myristate acetate (PMA)-differentiated U-937 cells. In contrast, all of the mutants replicated within
http://www.asmscience.org/content/book/10.1128/9781555817985.chap27
Environmental amoebae do not support the long-term survival of virulent mycobacteria - Mardare - 2013 - Journal of Applied...Environmental amoebae do not support the long-term survival of virulent mycobacteria - Mardare - 2013 - Journal of Applied...
In this study, we used a novel approach to detect internalized mycobacteria in environmental protozoa from badger latrines. Acid-fast micro-organisms were visualized in isolated amoebae, although we were unable to identify them to species level as no mycobacteria were grown from these samples nor was M. bovis detected by IS6110 PCR. Co-incubation of Acanthamoeba castellanii with virulent M. bovis substantially reduced levels of bacilli, indicating that the amoebae have a negative effect on the persistence of M. bovis. ...
http://onlinelibrary.wiley.com/doi/10.1111/jam.12166/abstract
Multiplication of different Legionella species in Mono Mac 6 cells and in Acanthamoeba castellanii. | Applied and Environmental...Multiplication of different Legionella species in Mono Mac 6 cells and in Acanthamoeba castellanii. | Applied and Environmental...
Survival and distribution of legionellae in the environment are assumed to be associated with their multiplication in amoebae, whereas the ability to multiply in macrophages is usually regarded to correspond to pathogenicity. Since most investigations focused on Legionella pneumophila serogroup 1, we examined the intracellular multiplication of different Legionella species in Mono Mac 6 cells, which express phenotypic and functional features of mature monocytes, and in Acanthamoeba castellanii, an environmental host of Legionella spp. According to the bacterial doubling time in Mono Mac 6 cells and in A. castellanii, seven clusters of legionellae could be defined which could be split further with regard to finer differences. L. longbeachae serogroup 1, L. jordanis, and L. anisa were not able to multiply in either A. castellanii or Mono Mac 6 cells and are members of the first cluster. L. dumoffi did not multiply in Mono Mac 6 cells but showed a delayed multiplication in A. castellanii 72 h after ...
https://aem.asm.org/content/63/4/1219?ijkey=908df181c6993a0d8fc204f0b424840f2212dccb&keytype2=tf_ipsecsha
Resistance, biguanide sorption and biguanide-induced pentose leakage during encystment of Acanthamoeba castellanii -ORCAResistance, biguanide sorption and biguanide-induced pentose leakage during encystment of Acanthamoeba castellanii -ORCA
Aims: This study investigates the effects of biguanides during encystment of Acanthamoeba castellanii. Methods and Results: A non-nutrient encystment system was used to investigate the changes in the levels of sorption (uptake) of three non-cysticidal concentrations (10, 20 and 50 μg ml−1) of chlorhexidine diacetate (CHA) and polyhexamethylene biguanide (PHMB) as well as their effects on viability and leakage of pentose sugars during the first 36 h of encystment. Trophozoites treated with CHA or PHMB were more sensitive and generally sorbed more of each biocide than cysts. During encystment, the largest increases in resistance developed between 18 and 36 h for both biguanides with the resistance emerging to biguanide concentrations of 10 or 20 μg ml−1 between 18 and 24 h. At 50 μg ml−1 resistance emerged between 24 and 36 h. There was a general decrease in biocide sorption during encystment between 0-24 and 0-21 h for CHA and PHMB, respectively, at a concentration of 50 μg ml−1. The ...
https://orca.cf.ac.uk/62671/
Efficacy of anti-neoplastic drugs against acanthamoeba<...Efficacy of anti-neoplastic drugs against acanthamoeba<...
TY - JOUR. T1 - Efficacy of anti-neoplastic drugs against acanthamoeba. AU - Beattie, T.K.. AU - Tomlinson, A.. AU - Seal, D.. PY - 2003. Y1 - 2003. N2 - Purpose:Late presenting cases of Acanthamoeba keratitis are particularly difficult to treat. The organism can persist, despite treatment with chlorhexidine or PHMB, maintaining an active infection. New drug therapies with enhanced activity are therefore needed to treat this painful, potentially blinding infection. This study was undertaken to determine the efficacy of three anti-cancer drugs against Acanthamoeba. Methods:Doubling dilutions of MGBG [10-0.15mg/ml (38.5-0.6mM)], CHS 828 [660-10µg/ml (1782-27µM)] and hexadecyl-phosphocholine [HePC, Miltefosine; 660-10µg/ml (1782-27µM)] were tested alone or in combination with chlorhexidine, PHMB and propamidine, for efficacy against Acanthamoeba castellanii trophozoites or cysts. Sensitivity assays were performed over 48h in 96-well microtitre plates. Results: MGBG killed trophozoites at ...
https://pureportal.strath.ac.uk/en/publications/efficacy-of-anti-neoplastic-drugs-against-acanthamoeba
Identification of Legionella pneumophilaGenes Important for Infection of Amoebas by Signature-Tagged Mutagenesis | Infection...Identification of Legionella pneumophilaGenes Important for Infection of Amoebas by Signature-Tagged Mutagenesis | Infection...
Legionella pneumophila is a facultative intracellular gram-negative rod that causes pneumonia in humans. Free-living amoebas are thought to serve as a reservoir for Legionellainfections. Signature-tagged mutagenesis was employed to identifyLegionella pneumophila genes necessary for survival in the amoeba Acanthamoeba castellanii. Six mutant strains were defective in assays of invasion and intracellular growth. Four mutants also exhibited invasion and replication defects in Hartmannella vermiformis, an amoeba linked to hospital outbreaks ofLegionella pneumonia. The six mutants also were tested in macrophages derived from peripheral blood mononuclear cells. Two mutants had intracellular replication defects, and two different strains entered cells less efficiently. Two transposon insertions were in known L. pneumophila genes, lspK andaroB. The other four were in novel genes. One gene has similarity to a cytochrome c-type biogenesis protein ofPseudomonas fluorescens. Another has similarity to a ...
https://iai.asm.org/content/69/2/977.abstract
Meňavky rodu Acanthamoeba - pôvodcovia infekcií človeka | proLékaře.czMeňavky rodu Acanthamoeba - pôvodcovia infekcií človeka | proLékaře.cz
1. Adl, S. M., Simpson, A. G. B., Farmer, M. A., Andersen, R. A. et al. The new higher level classification of eukaryotes with emphasis on the taxonomy of protists. J. Eukaryot. Microbiol., 2005, 52, p. 399-451. 2. Aichelburg, A. C., Walochnik, J., Assadian, O., Prosch, H. et al. Successful treatment of disseminated Acanthamoeba sp. infection with miltefosine. Emerg. Infect. Dis., 2008, 14, p. 1743-1746. 3. Booton, G. C., Visvesvara, G. S., Byers, T. J., Kelly, D. J. et al. Identification and distribution of Acanthamoeba species genotypes associated with nonkeratitis infections. J. Clin. Microbiol., 2005, 43, p. 1689-693. 4. Cengiz, A. M., Harmis, N., Stapleton, F. Co-incubation of Acanthamoeba castellanii with strains of Pseudomonas aeruginosa alters the survival of amoeba. Clin. Exp. Ophthalmol., 2000, 28, p. 191-193. 5. Chatton, E. Classe des Lobosa Leidy, 1879. Ordre des amoebiens nus ou Amoebaea. In Grassé, P. P. Traité de zoologie, anatomie, systématique, biologie. Tome I. Protozoaires. ...
https://www.prolekare.cz/casopisy/epidemiologie/2011-3/menavky-rodu-acanthamoeba-povodcovia-infekcii-cloveka-36183
Interactions of the ubiquitous amoeba Acanthamoeba polyphaga with a plethora of pathogenic bacteria - Queens University...
Powered by Pure, Scopus & Elsevier Fingerprint Engine™ © 2020 Elsevier B.V. We use cookies to help provide and enhance our service and tailor content. By continuing you agree to the use of cookies. Log in to Pure. ...
https://pure.qub.ac.uk/en/activities/interactions-of-the-ubiquitous-amoeba-acanthamoeba-polyphaga-with
Gene switching in Amoeba proteus caused by endosymbiotic bacteria | Journal of Cell ScienceGene switching in Amoeba proteus caused by endosymbiotic bacteria | Journal of Cell Science
Fig. 7. Alignment of the deduced amino acid sequence of amoeba SAMS2 with SAMS sequences of other organisms. Periods represent amino acids identical to those of MetK of E. coli, and dashes show gaps inserted for an optimal alignment of amino acids. *1, ATP-binding motif; *2, glycine-rich nanopeptide; *3, metal-binding sites; *4, a site for Cys-121 of human MAT1A, characteristic of liver enzymes; EC, E. coli; XB, X-bacteria; AC, Acanthamoeba castellanii; S2, SAMS2 of amoebae; PI, Phytophthora infestans; AP, Amoeba proteus. Complete genomic or cDNA sequences of SAMS proteins are available in GenBank, for E. coli (accession no. 1708999), A. castellanii (6016547), and P. infestans (23394401). Amino acid sequences of amoeba SAMS1 (U91602) and X-bacteria SAMS (AY324627) are from Choi et al. (Choi et al., 1997) and Jeon and Jeon (Jeon and Jeon, 2003).. ...
http://jcs.biologists.org/content/117/4/535.figures-only
Search Results | Journal of Cell Biology | Rockefeller University PressSearch Results | Journal of Cell Biology | Rockefeller University Press
The Acanthamoeba castellanii myosin-Is were the first unconventional myosins to be discovered, and the myosin-I class has since been found to be one of the more diverse and abundant classes of the myosin superfamily. We used two-dimensional (2D) crystallization on phospholipid monolayers and negative stain electron microscopy to calculate a projection map of a classical myosin-I, Acanthamoeba myosin-IB (MIB), at ∼18 Å resolution. Interpretation of the projection map suggests that the MIB molecules sit upright on the membrane. We also used cryoelectron microscopy and helical image analysis to determine the three-dimensional structure of actin filaments decorated with unphosphorylated (inactive) MIB. The catalytic domain is similar to that of other myosins, whereas the large carboxy-terminal tail domain differs greatly from brush border myosin-I (BBM-I), another member of the myosin-I class. These differences may be relevant to the distinct cellular functions of these two types of myosin-I. ...
https://rupress.org/jcb/search-results?f_Authors=E.+Michael+Ostap
UniProt/TrEMBL: L8GWF8 ACACA
ID L8GWF8_ACACA Unreviewed; 916 AA. AC L8GWF8; DT 03-APR-2013, integrated into UniProtKB/TrEMBL. DT 03-APR-2013, sequence version 1. DT 22-NOV-2017, entry version 16. DE SubName: Full=Phosphoenolpyruvate carboxylase {ECO:0000313,EMBL:ELR17267.1}; GN ORFNames=ACA1_059840 {ECO:0000313,EMBL:ELR17267.1}; OS Acanthamoeba castellanii str. Neff. OC Eukaryota; Amoebozoa; Discosea; Longamoebia; Centramoebida; OC Acanthamoebidae; Acanthamoeba. OX NCBI_TaxID=1257118 {ECO:0000313,EMBL:ELR17267.1, ECO:0000313,Proteomes:UP000011083}; RN [1] {ECO:0000313,EMBL:ELR17267.1, ECO:0000313,Proteomes:UP000011083} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Neff {ECO:0000313,EMBL:ELR17267.1, RC ECO:0000313,Proteomes:UP000011083}; RX PubMed=23375108; DOI=10.1186/gb-2013-14-2-r11; RA Clarke M., Lohan A.J., Liu B., Lagkouvardos I., Roy S., Zafar N., RA Bertelli C., Schilde C., Kianianmomeni A., Burglin T.R., Frech C., RA Turcotte B., Kopec K.O., Synnott J.M., Choo C., Paponov I., RA Finkler A., Soon Heng ...
http://www.genome.jp/dbget-bin/www_bget?tr:L8GWF8_ACACA
Intermediary metabolism in protists : a sequence-based view of facultative anaerobic metabolism in evolutionarily diverse...Intermediary metabolism in protists : a sequence-based view of facultative anaerobic metabolism in evolutionarily diverse...
Protists account for the bulk of eukaryotic diversity. Through studies of gene and especially genome sequences the molecular basis for this diversity can be determined. Evident from genome sequencing are examples of versatile metabolism that go far beyond the canonical pathways described for eukaryotes in textbooks. In the last 2-3 years, genome sequencing and transcript profiling has unveiled several examples of heterotrophic and phototrophic protists that are unexpectedly well-equipped for ATP production using a facultative anaerobic metabolism, including some protists that can (Chlamydomonas reinhardtii) or are predicted (Naegleria gruberi, Acanthamoeba castellanii, Amoebidium parasiticum) to produce H(2) in their metabolism. It is possible that some enzymes of anaerobic metabolism were acquired and distributed among eukaryotes by lateral transfer, but it is also likely that the common ancestor of eukaryotes already had far more metabolic versatility than was widely thought a few years ago. ...
http://eprints.lancs.ac.uk/34903/
Most recent papers with the keyword S. pneumoniae | Read by QxMDMost recent papers with the keyword S. pneumoniae | Read by QxMD
Here, we hypothesized that the microbial gut flora of animals/pests living in polluted environments, produce substances to thwart bacterial infections. The overall aim of this study was to source microbes inhabiting unusual environmental niches for potential antimicrobial activity. Two cockroach species, Gromphadorhina portentosa (Madagascar) and Blaptica dubia (Dubia) were selected. The gut bacteria from these species were isolated and grown in RPMI 1640 and conditioned media were prepared. Conditioned media were tested against a panel of Gram-positive (Methicillin resistant Staphylococcus aureus, Streptococcus pyogenes, Bacillus cereus) and Gram-negative (Escherichia coli K1, Salmonella enterica, Serratia marcescens, Pseudomonas aeruginosa, Klebsiella pneumoniae) bacteria, as well as the protist pathogen, Acanthamoeba castellanii ...
https://www.readbyqxmd.com/keyword/80830
LQs taking a long time to molt?LQs taking a long time to molt?
So all five of my LQs are in premolt, and have been for months now. The temps are around 80F and I LIGHTLY mist them every week or two. Some eat and some dont.
http://scorpionforum.darkbb.com/t4232-lqs-taking-a-long-time-to-molt
LQS (Space Qualification Lab) - EREALQS (Space Qualification Lab) - EREA
Facility aimed at supporting aerospace companies in the design, manufacturing and testing of nano and micro satellites (up to 70 Kg) and electronic and…
https://erea.org/catalogue/listing/lqs-space-qualification-lab/
Campylobacter jejuni Actively Invades the Amoeba Acanthamoeba polyphaga and Survives within Non Digestive VacuolesCampylobacter jejuni Actively Invades the Amoeba Acanthamoeba polyphaga and Survives within Non Digestive Vacuoles
The Gram-negative bacterium Campylobacter jejuni is able to enter, survive and multiply within the free living amoeba Acanthamoeba polyphaga, but the molecular mechanisms behind these events are still unclear. We have studied the uptake and intracellular trafficking of viable and heat killed bacterial cells of the C. jejuni strain 81-176 in A. polyphaga. We found that viable bacteria associated with a substantially higher proportion of Acanthamoeba trophozoites than heat killed bacteria. Furthermore, the kinetics of internalization, the total number of internalized bacteria as well as the intracellular localization of internalized C. jejuni were dramatically influenced by bacterial viability. Viable bacteria were internalized at a high rate already after 1 h of co-incubation and were observed in small vacuoles tightly surrounding the bacteria. In contrast, internalization of heat killed C. jejuni was low at early time points and did not peak until 96 h. These cells were gathered in large ...
http://www.diva-portal.org/smash/record.jsf?pid=diva2:677424
New tool for the simultaneous detection of ten genotypes of Acanthamoeba | British Journal of OphthalmologyNew tool for the simultaneous detection of ten genotypes of Acanthamoeba | British Journal of Ophthalmology
New tool for the simultaneous detection of ten different genotypes of Acanthamoeba available from the American Type Culture Collection. Background: Acanthamoeba keratitis (AK) is a sight-threatening infection and none of the current diagnosis tests are able to detect in one reaction low levels of the vast majority of strains associated with pathology. The goal of this work was to validate a new tool for the detection of the American Type Cell Collection referenced Acanthamoeba monitoring simultaneously DNA extraction yields and PCR inhibitors. Performances were assessed on corneal scrapings.. Methods: Primers were selected in a region bracketing a 41591 bp of the A. castellanii mitochondrion gene. DNA extraction and PCR inhibitors were monitored by adding an internal control (virus). Acanthamoeba were detected and quantified by the real time fast duplex TaqMan® PCR (f-d-real-t PCR) and negativity confirmed by SYBR Green real time PCR.. Results: The f-d-real-t PCR detects 0.1 cyst/µl or less of ...
http://bjo.bmj.com/content/early/2009/04/28/bjo.2008.144873
Two small ncRNAs jointly govern virulence and transmission in Legionella pneumophila. - Semantic ScholarTwo small ncRNAs jointly govern virulence and transmission in Legionella pneumophila. - Semantic Scholar
To transit from intra- to extracellular environments, Legionella pneumophila differentiates from a replicative/non-virulent to a transmissive/virulent form using the two-component system LetA/LetS and the global repressor protein CsrA. While investigating how both regulators act co-ordinately we characterized two ncRNAs, RsmY and RsmZ, that link the LetA/LetS and CsrA regulatory networks. We demonstrate that LetA directly regulates their expression and show that RsmY and RsmZ are functional in Escherichia coli and are able to bind CsrA in vitro. Single mutants have no (ΔrsmY) or a little (ΔrsmZ) impact on virulence, but the ΔrsmYZ strain shows a drastic defect in intracellular growth in Acanthamoeba castellanii and THP-1 monocyte-derived macrophages. Analysis of the transcriptional programmes of the ΔletA, ΔletS and ΔrsmYZ strains revealed that the switch to the transmissive phase is partially blocked. One major difference between the ΔletA, ΔletS and ΔrsmYZ strains was that the latter
https://www.semanticscholar.org/paper/Two-small-ncRNAs-jointly-govern-virulence-and-tran-Sahr-Br%C3%BCggemann/c1ab63d1268a0827aae1ca045018b23ab2eb0a29
Acanthamoeba - WikipediaAcanthamoeba - Wikipedia
Acanthamoeba is a genus of amoebae, a single-celled eukaryote commonly recovered from soil, fresh water and other habitats. Acanthamoeba has two evolutive forms, the metabolically active trophozoite and a dormant, stress resistant cyst. Trophozoites are small, usually 15 to 35 μm in length and amoeboid in shape. In nature, Acanthamoeba species are free-living bacterivores, but in certain situations they can cause infections (Acanthamebiasis) in humans and other animals. Acanthamoeba spp. are among the most prevalent protozoa found in the environment. They are distributed worldwide, and have been isolated from soil, air, sewage, seawater, chlorinated swimming pools, domestic tap water, bottled water, dental treatment units, hospitals, air-conditioning units, and contact lens cases. Additionally, Acanthamoeba have been isolated from human skin, nasal cavities, throats, and intestines, as well as plants and other mammals. Diseases caused by Acanthamoeba include keratitis and granulomatous amoebic ...
https://en.wikipedia.org/wiki/Acanthamoeba
Diagnosis of Acanthamoeba keratitis in vivo with confocal microscopy. - Semantic ScholarDiagnosis of Acanthamoeba keratitis in vivo with confocal microscopy. - Semantic Scholar
We present eight cases of Acanthamoeba keratitis. In each case; the Acanthamoeba organisms were visualized in the epithelium and anterior stroma using tandem scanning confocal microscopy. The organisms were highly reflective, ovoid, and were 10-25 microns in diameter. The Acanthamoeba organisms in the human corneas were identical in size and shape to Acanthamoeba organisms on an agar plate visualized with the same confocal microscope. Confocal microscopy is a useful method for identifying Acanthamoeba organisms in vivo within the corneal epithelium and anterior stroma.
https://www.semanticscholar.org/paper/Diagnosis-of-Acanthamoeba-keratitis-in-vivo-with-c-Winchester-Mathers/c024bcaa5ccf77e4b237b721f376da48df7631bc
CEA - Commissariat à lénergie atomique - In-depth study of Mollivirus sibericum , a new 30,000-y-old giant virus infecting...CEA - Commissariat à lénergie atomique - In-depth study of Mollivirus sibericum , a new 30,000-y-old giant virus infecting...
Acanthamoeba species are infected by the largest known DNA viruses. These include icosahedral Mimiviruses, amphora-shaped Pandoraviruses, and Pithovirus sibericum, the latter one isolated from 30,000-y-old permafrost. Mollivirus sibericum, a fourth type of giant virus, was isolated from the same permafrost sample. Its approximately spherical virion (0.6-µm diameter) encloses a 651-kb GC-rich genome encoding 523 proteins of which 64% are ORFans; 16% have their closest homolog in Pandoraviruses and 10% in Acanthamoeba castellanii probably through horizontal gene transfer. The Mollivirus nucleocytoplasmic replication cycle was analyzed using a combination of
https://hal-cea.archives-ouvertes.fr/cea-01825900?frbrVersion=6
permafrostpermafrost
A new virus called Pithovirus sibericum has been isolated from 30,000 year old Siberian permafrost. It is the oldest DNA virus of eukaryotes ever isolated, showing that viruses can retain infectivity in nature for very long periods of time.. Pithovirus was isolated by inoculating cultures of the amoeba Acanthamoeba castellani with samples taken in the year 2000 from 30 meters below the surface of a late Pleistocene sediment in the Kolyma lowland region. This amoeba had been previously used to propagate other giant viruses, such as Mimivirus and Pandoravirus. Light microscopy of the cultures revealed the presence of ovoid particles which were subsequently shown by electron microscopy to resemble those of Pandoravirus. Pithovirus particles are flask-shaped and slightly larger than Pandoravirus - 1.5 microns long, 500 nm in diameter, encased by a 60 nm thick membrane. One end of the virus particle appears to be sealed with what the authors call a cork (photo). This feature, along with the shape of ...
http://www.virology.ws/tag/permafrost/
Acanthamoeba mauritaniensis Pussard and Pons ATCC ® 50679™Acanthamoeba mauritaniensis Pussard and Pons ATCC ® 50679™
Acanthamoeba mauritaniensis ATCC ® 50679™ Designation: SAWE94/4 Isolation: From eye of human with Acanthamoeba keratitis, South Africa, 1994
https://www.atcc.org/en/Products/Cells_and_Microorganisms/Protozoa/Protozoa_Alphanumeric/50679.aspx
Viscometric analysis of the gelation of Acanthamoeba extracts and purification of two gelation factors. | JCBViscometric analysis of the gelation of Acanthamoeba extracts and purification of two gelation factors. | JCB
We have studied the kinetics of the gelation process that occurs upon warming cold extracts of Acanthamoeba using a low-shear falling ball assay. We find that the reaction has at least two steps, requires 0.5 mM ATP and 1.5 mM MgCl2, and is inhibited by micromolar Ca++. The optimum pH is 7.0 and temperature, 25 degrees-30 degrees C. The rate of the reaction is increased by cold preincubation with both MgCl2 and ATP. Nonhydrolyzable analogues of ATP will not substitute for ATP either in this potentiation reaction or in the gelation process. Either of two purified or any one of four partially purified Acanthamoeba proteins will cross-link purified actin to form a gel, but none can account for the dependence of the reaction in the crude extract on Mg-ATP or its regulation by Ca++. This suggests that the extract contains, in addition to actin-cross-linking proteins, factors dependent on Mg-ATP and Ca++ that regulate the gelation process. ...
http://jcb.rupress.org/content/85/2/414.long
management | LQS Expertmanagement | LQS Expert
Get ready! The truth be advised, this career path just isnt for people with weak knees. This coaching includes medical terminology, computer science, radiation safety, scientific theories, study of the Continue Reading ». ...
https://www.loquenosabias.net/tag/management
CDB Chalk Talk: Good / Little LabsCDB Chalk Talk: Good / Little Labs
Good-Little .Chalk Talk. Open to the PSOM community.. https://pennmedicine.zoom.us/j/95672662676?pwd=ejNza3lQS1dVOUpVVVlkV2pYOUVSZz09. Meeting ID: 956 7266 ...
https://cdb.med.upenn.edu/event/cdb-chalk-talk-good-little-labs/
I have Fibromyalgia and a dumb question..I have Fibromyalgia and a dumb question..
I am finding it harder and harder to cut my fabric, my hand gets tired and my arms get tried with the rotary cutter. Will most LQS cut MY fabric into
https://www.quiltingboard.com/main-f1/i-have-fibromyalgia-dumb-question-t18045.html
Acanthamoeba Infection Market Innovative Treatments, Key Methodologies, Top Players Success Milestones and Application...
The recent study, Acanthamoeba Infection market forecasts the business performance of the Acanthamoeba Infection market for the forecast period, 2019 to 2026. The study considers the estimated period as the base duration and brings to light the important information associated with the market size, share and growth rate of the Acanthamoeba Infection market.. Apart from this, the research closely examines the market share occupied by some of the prominent market players in the Acanthamoeba Infection market for the forecast period, 2019 to 2026. Researchers behind the research unmask vital statistics on market segmentation including product types, application, sale and geography.. FREE SAMPLE COPY [Customized Copy + CAGR and Gross Market Value Included] @ https://www.marketexpertz.com/sample-enquiry-form/74658. This study relies on the evolution of the industry to derive the trends that are observed. A significant increase in the global market is visible, which promises the expansion of the market ...
http://themarketstrategies.com/acanthamoeba-infection-market-innovative-treatments-key-methodologies-top-players-success-milestones-and-application-forecasts-to-2026/
Acanthamoeba Infection: Evaluation
Another name for Acanthamoeba Infection is Acanthamoeba Infection. The evaluation of an acanthamoeba infection begins with a history and physical examination ...
http://www.freemd.com/acanthamoeba-infection/evaluation.htm
Contagious: Acanthamoeba infection of the eye - RightDiagnosis.comContagious: Acanthamoeba infection of the eye - RightDiagnosis.com
Acanthamoeba infection of the eye is it contagious? Contagiousness of Acanthamoeba infection of the eye including infectiousness, transmission, and contagion methods and vectors.
http://www.rightdiagnosis.com/a/acanthamoeba_infection_of_the_eye/contagious.htm
Parasite of the Day: Acanthamoeba spp.Parasite of the Day: Acanthamoeba spp.
Acanthamoeba spp. are microscopic organisms that can be found just about anywhere, from soil to water, to the air we breathe. They are the direct culprits of Acanthamoeba keratitis (AK) a relatively rare but sight-threatening disease which is actually caused by at least eight species of Acanthamoeba: A. castellanii, A. culbertsoni, A. polyphaga, A. hatchetti, A. rhysodes, A. lugdunesis, A. quina, and A. griffin. Ocular trauma and contaminated water are also associated with AK infections but it has been found that contact lens wearing accounts for , 80% of the cases. If found early the infection can be cured, but this gets progressively more difficult the longer it remains untreated. The difficulty lies with the life cycle of the Acanthamoeba species which consists of two stages: the trophozoite and the cyst ...
http://dailyparasite.blogspot.com/2019/02/acanthamoeba-spp.html
Eye ParasitesEye Parasites
A. castellanii is a ubiquitous organism, found in many ecosystems worldwide. It is able to survive in harsh environmental circumstances - even in some contact lens solutions - and this is not the first occurrence of A. castellanii appearing in the eye. Acanthamoeba keratitis (AK) is a neglected malady frequently associated with contact lens wear.. Acanthamoeba infection of the cornea causes severe inflammation, intense pain and impaired vision, which is blinding if left untreated. Infection begins when the parasite is at its active feeding trophozoite stage and sticks to the corneal tissue before penetrating the lower stromal layer. The resulting opacity leads to less sharp vision and eventually blindness.. Acanthamoeba infections (not just in the eye) are being detected by clinicians with increasing frequency, especially as opportunistic infections in patients whose immune system is already compromised. This at-risk population is expanding as a result of increasing use of immune-suppressing ...
https://www.omicsonline.org/conferences-list/eye-parasites
ASMscience | Interaction of Legionell
Several pathogens exhibit a considerable host range. Legionella pneumophila, for example, can infect various protozoa species, experimentally inoculated guinea pigs, and human macrophages, as well as epithelial cells. Vegetative cells of Dictyostelium feed on bacteria and upon starvation aggregate and differentiate into pluricellular fruiting bodies. To evaluate whether D. discoideum is a suitable model system for studying Legionella pathogenicity, the authors compared the intracellular growth of different Legionella species in Dictyostelium with the established host model system Acanthamoeba castellanii. The FlaA-and the Mip-negative mutant of L. pneumophila Corby revealed moderate growth defects and the ligA-negative mutant was severely impaired to grow intracellularly. To examine host functions required for growth, the authors also investigated defined Dictyostelium mutants. Identification of Legionella spp., Dictyostelium spp., and Hartmannella spp. by fluorescence-labeled rRNA probes have been
http://www.asmscience.org/content/book/10.1128/9781555817985.chap28
Further Departments and Research GroupsFurther Departments and Research Groups
Legionella pneumophila is the causative agent of Legionnaires disease. Due to the hot climate and intermittent water supply, the West Bank, Palestine, can be considered a high-risk area for this often fatal atypical pneumonia. L. pneumophila occurs in biofilms of natural and man-made freshwater environments, where it infects and replicates intracellularly within protozoa. To correlate the genetic diversity of the bacteria in the environment with their virulence properties for protozoan and mammalian host cells, 60 genotyped isolates from hospital water systems in the West Bank were analyzed. The L. pneumophila isolates were previously genotyped by high resolution Multi Locus Variable Number of Tandem Repeat Analysis (MLVA-8(12)) and sorted according to their relationship in clonal complexes (VACC). Strains of relevant genotypes and VACCs were compared according to their capacity to infect Acanthamoeba castellanii and THP-1 macrophages, and to mediate pore-forming cytotoxicity in sheep red blood ...
https://repository.helmholtz-hzi.de/handle/10033/6839
Rat myr 4 defines a novel subclass of myosin I: identification, distribution, localization, and mapping of calmodulin-binding...Rat myr 4 defines a novel subclass of myosin I: identification, distribution, localization, and mapping of calmodulin-binding...
We report the identification and characterization of myr 4 (myosin from rat), the first mammalian myosin I that is not closely related to brush border myosin I. Myr 4 contains a myosin head (motor) domain, a regulatory domain with light chain binding sites and a tail domain. Sequence analysis of myosin I head (motor) domains suggested that myr 4 defines a novel subclass of myosin Is. This subclass is clearly different from the vertebrate brush border myosin I subclass (which includes myr 1) and the myosin I subclass(es) identified from Acanthamoeba castellanii and Dictyostelium discoideum. In accordance with this notion, a detailed sequence analysis of all myosin I tail domains revealed that the myr 4 tail is unique, except for a newly identified myosin I tail homology motif detected in all myosin I tail sequences. The Ca(2+)-binding protein calmodulin was demonstrated to be associated with myr 4. Calmodulin binding activity of myr 4 was mapped by gel overlay assays to the two consecutive light ...
https://rupress.org/jcb/article/126/2/375/29118/Rat-myr-4-defines-a-novel-subclass-of-myosin-I
Acanthamoeba Infections | Quick Medical Diagnosis & Treatment 2021 | AccessMedicine | McGraw-Hill MedicalAcanthamoeba Infections | Quick Medical Diagnosis & Treatment 2021 | AccessMedicine | McGraw-Hill Medical
Acanthamoeba Infections. In: Papadakis MA, McPhee SJ, Bernstein J. Papadakis M.A., & McPhee S.J., & Bernstein J(Eds.),Eds. Maxine A. Papadakis, et al.eds. Quick Medical Diagnosis & Treatment 2021. McGraw-Hill; Accessed November 26, 2020. https://accessmedicine.mhmedical.com/content.aspx?bookid=2986§ionid=251085632 ...
https://accessmedicine.mhmedical.com/content.aspx?bookid=2986§ionid=251085632&TopLevelContentDisplayName=Quick%20Reference
Survival and Distribution of Fusarium and Acanthamoeba in Drying MPS | IOVS | ARVO JournalsSurvival and Distribution of Fusarium and Acanthamoeba in Drying MPS | IOVS | ARVO Journals
Purpose: : To examine further the association between the recent outbreaks of microbial keratitis caused by unusual organisms and the contamination of multipurpose contact lens disinfection solutions (MPS) during use. Methods: : Conidia of representative isolates of the Fuarium solani-F. oxysporum complexes and trophozoites of Acanthamoeba castelanii were inoculated (~104 ml-1) into 1-2 ml of MPS that contained either polyhexamethylene biguanide (PHMB) or polyquaternium-1 (PQ) as active disinfecting components. Phosphate-buffered saline (PBS) served as a control. These preparations were examined microscopically during drying under a laminar flow hood at ambient temperatures. Results: : The various MPS formed distinct and characteristic concentric bands of precipitates and viscous globules while drying. The distribution of the conidia, trophozoites and amoebic cysts among the distinctive residual- precipitate patterns varied for the different MPS. Cysts were not evident in the initial trophozoite ...
https://iovs.arvojournals.org/article.aspx?articleid=2380902
Acanthamoeba: A rare and potentially blinding parasite - Outbreak News TodayAcanthamoeba: A rare and potentially blinding parasite - Outbreak News Today
There are three main genera of free living amoeba that infect humans - Acanthamoeba, Balamuthia, Naegleria that are are important causes of disease in humans and animals. The topic for todays podcast will be one of them- Acanthamoeba.. Acanthamoeba is a microscopic, free-living amoeba that can cause rare, but severe infections of the eye, skin, and central nervous system.. Joining me to talk about Acanthamoeba is Parasitology teacher and author of Parasites: Tales of Humanitys Most Unwelcome Guests, Rosemary Drisdelle.. ...
http://outbreaknewstoday.com/acanthamoeba-rare-potentially-blinding-parasite-47485/
Avaliação fisiológica, morfológica e caracterização imunoquímica de Acanthamoeba por anticorpos policlonais e monoclonaisAvaliação fisiológica, morfológica e caracterização imunoquímica de Acanthamoeba por anticorpos policlonais e monoclonais
Abstract: The free-living amoebae of the genus Acanthamoeba are widely distributed in nature and are considered potentially pathogenic organisms. Occasionally they can trigger human infections such as granulomatous amoebic encephalitis and amoebic keratitis. The investigation of differentiating characteristics between pathogenic strains and those not associated with infection may help to determine factors related to pathogenicity and the development of diagnostic tests. In this sense, the aim of this study was to perform a comparative evaluation; by means of physiological, morphological and immunochemical criteria; between clinical and environmental samples of Acanthamoeba. Trophozoites of four isolates were used: a clinical sample, obtained from a confirmed case of amoebic keratitis; an environmental sample, obtained from the dust of the residence of the same patient; and two reference samples A. poliphaga #2, obtained from an amoebic keratitis (ATCC 30641) and A. poliphaga #4, obtained from ...
https://acervodigital.ufpr.br/handle/1884/46495
Pathogenesis of Acanthamoeba Keratitis - Noorjahan PanjwaniPathogenesis of Acanthamoeba Keratitis - Noorjahan Panjwani
Acanthamoeba keratitis (AK) is a serious, debilitating, and intensely painful infection of the cornea caused by parasites of the genus Acanthamoeba. At present,...
http://grantome.com/grant/NIH/R01-EY009349-13
ما هي الاكانثاميبا Acanthamoeba|اسبابها|الاعراض|العلاج | Medicine, science and more
تعالى نفصصهم تاني … المرض الأول اسمه Acanthamoeba keratitis: وده بيعمل التهاب في قرنيه العين وده عامل كانه سدلك العدسة اللى بتصور بيها يعنى ممكن يسبب العمى.. المرض التاني Granulomatous Amebic Encephalitis: وده بيعمل التهاب في المخ والحبل الشوكي وده معناه انه ممكن يقتل رئيس دولتك يعنى يموت المخ والحبل الشوكي وده معناه انهيار الدولة كلها يعنى يؤدي للموت. المرض التالت واسمه disseminated infection: وده ذي ما قولنا عامل ذي الغزو بينتشر في كل حتة وبيعمل التهابات في كل حتة.. You can say This means that it is dangerous and can kill me and can attack me at any moment and you told me that this enemy lives everywhere… this is scary.. No, dont worry. It is rare to attack you because it is a weak ...
https://medicine-science-and-more.com/what-is-acanthamoeba-infectioncausessymptomstreatment25/
A Specific Genomic Location within the icm/dot Pathogenesis Region of Different Legionella Species Encodes Functionally Similar...A Specific Genomic Location within the icm/dot Pathogenesis Region of Different Legionella Species Encodes Functionally Similar...
L. pneumophila grows intracellularly and kills the host cell due to the activity of the Icm/Dot type IV secretion system, which is believed to form a protein complex that translocates effector proteins from the bacterial cytoplasm into the host cell. The icm/dot system was recently discovered also in C. burnetii and included all of the icm/dot genes except for icmR (30, 31). The icmR gene, together with icmQLp, is located in region IIa; their gene products are predicted to be found in the bacterial cytoplasm and are not homologous to conjugation-related proteins encoded by plasmid R64. This information, together with the finding that C. burnetii contains the entire icm/dot system except for icmR, led us to focus on region IIa as a potential cause for differences between bacteria that contain the Icm/Dot virulence system.. Besides L. pneumophila, several pathogenic Legionella species have been described, and we chose to study two of them: L. micdadei which is the second most common agent of ...
https://iai.asm.org/content/72/8/4503
A case of medication-resistant acanthamoeba keratitis treated by corneal crosslinking in Turkey.
Purpose. To report a case of medication-resistant acanthamoeba keratitis (AK) treated successfully by corneal crosslinking (CXL). Methods. A 26-year-old male with medication-resistant AK underwent a standard CXL procedure with local anesthesia, follo
http://www.biomedsearch.com/nih/case-medication-resistant-acanthamoeba-keratitis/24455365.html
Genotyping determination of Acanthamoeba strains: an original study and a systematic review in Iran.Genotyping determination of Acanthamoeba strains: an original study and a systematic review in Iran.
This study aimed to detect the presence of Acanthamoeba spp. in different water resources of Zahedan, southeast of Iran, and also systematically reviewed all publications regarding Acanthamoeba in Iran (2005-2018). Fifty water samples were collected from different water resources in Zahedan. The positive samples were identified morphologically and subjected to polymerase chain reaction (PCR) using...
https://medworm.com/737716640/genotyping-determination-of-acanthamoeba-strains-an-original-study-and-a-systematic-review-in-iran/
Acanthamoeba sp. ATCC ® PRA-219™Acanthamoeba sp. ATCC ® PRA-219™
Acanthamoeba sp. ATCC ® PRA-219™ Designation: UWC1/UV-7 Isolation: Acanthamoeba sp. UWC1 coincubated with activated sludge. Plattling, Bavaria, Germany.
https://www.atcc.org/Products/Cells_and_Microorganisms/Protozoa/Protozoa_Alphanumeric/PRA-219.aspx
Timeline: Contact solution maker failed to report | AMO Complete Moisture Plus Recall - SCHMIDT LLP see: The Schmidt Firm, PLLCTimeline: Contact solution maker failed to report | AMO Complete Moisture Plus Recall - SCHMIDT LLP see: The Schmidt Firm, PLLC
Key dates in the outbreak of eye infections linked to Advanced Medical Optics Complete MoisturePlus solution:. Feb. 2006- Nov. 2006: AMO receives nine reports involving patients infected with Acanthamoeba keratitis that go unreported.. March 2007: The CDC opens a multistate investigation into unusually high rates of the Acanthamoeba infection, which can result in permanent vision loss or blindness.. May 25, 2007: The CDC links AMOs Complete MoisturePlus to the outbreak and tells consumers to throw the product away.. May 26, 2007: AMO voluntarily recalls the solution under pressure from regulators at the CDC and the FDA.. May 29, 2007 to June 25, 2007: FDA inspectors arrive to investigate AMOs Irvine, Calif.-based headquarters. While there the FDA uncovers a series of problems, including the nine unreported complaint forms, which are finally submitted to the FDA.. Summer 2007: FDA officials schedule a meeting to discuss the problems uncovered at AMOs plant. No disciplinary action is ...
https://www.schmidtlaw.com/timeline-contact-solution-maker-failed-to-report/
Acanthamoeba - pathogen and vector of highly pathogenic bacteria strains to healthy and immunocompromised individuals / -...Acanthamoeba - pathogen and vector of highly pathogenic bacteria strains to healthy and immunocompromised individuals / -...
Acanthamoeba - pathogen and vector of highly pathogenic bacteria strains to healthy and immunocompromised individuals / - BAZA PUBLIKACJI PRACOWNIKÓW US
https://publi.bg.szczecin.pl/cgi-bin/koha/opac-detail.pl?bib=66308
Aimee Ahpeatone, LADC | Oklahoma City, OK | HealthgradesAimee Ahpeatone, LADC | Oklahoma City, OK | Healthgrades
Aimee Ahpeatone, LADC is a Counselor in Oklahoma City, OK. She has indicated that she accepts telehealth appointments. Be sure to call ahead with Aimee Ahpeatone to book an appointment.
https://www.healthgrades.com/providers/aimee-ahpeatone-3g4ry
DNA Extraction - Biology-OnlineDNA Extraction - Biology-Online
Hi, I am using QIAamp kit to extract DNA from Acanthamoeba culture. However, I just able to extract about 40ng/ul of concentration only when measured by NanoQuant. Is this concentration normal for Acanthamoeba ? This is because previously extraction from other sample can obtain high concentration up to 300ng/ul. Or is there any pre-treatment required to be done to the sample before using this Qiagen kit? I am sure there is no other step stated in Qiagen handbook, just that any other step that is specifically to acanthamoeba ...
https://www.biology-online.org/biology-forum/viewtopic.php?p=122532
American Journal of Ophthalmology - American Academy of OphthalmologyAmerican Journal of Ophthalmology - American Academy of Ophthalmology
Driving performance of patients with bilateral glaucoma. Photodynamic therapy to inhibit MRSA keratitis isolates. Refusal of surgical treatment for the fellow e
https://www.aao.org/eyenet/article/american-journal-of-ophthalmology-18
Prevalence of Acanthamoeba from Tap Water in Rio Grande do Sul, BrazilPrevalence of Acanthamoeba from Tap Water in Rio Grande do Sul, Brazil
A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.
https://www.growkudos.com/publications/10.1007%25252Fs00284-011-0003-5/reader
MarseilleviridaeMarseilleviridae
A second member is Acanthamoeba castellanii lausannevirus. Two additional viruses have been isolated but have yet to be named. ... The first member of this family recognized has been named Acanthamoeba polyphaga marseillevirus. ...
https://en.wikipedia.org/wiki/Marseilleviridae
UTP-monosaccharide-1-phosphate uridylyltransferaseUTP-monosaccharide-1-phosphate uridylyltransferase
Rudick VL, Weisman RA (1974). "Uridine diphosphate glucose pyrophosphorylase of Acanthamoeba castellanii. Purification, kinetic ...
https://en.wikipedia.org/wiki/UTP-monosaccharide-1-phosphate_uridylyltransferase
AcanthamoebaAcanthamoeba
... castellanii. Blocking these muscarinic receptors in past studies has proven to be amoebicidal in Acanthamoeba spp. More ... Acanthamoeba is a genus of amoebae that are commonly recovered from soil, fresh water, and other habitats. Acanthamoeba has two ... Acanthamoeba infection Balamuthia mandrillaris Marciano-Cabral F, Cabral G (April 2003). "Acanthamoeba spp. as agents of ... citing public domain text from the CDC Acanthamoeba - Centers for Disease Control and Prevention Video of Acanthamoeba from ...
https://en.wikipedia.org/wiki/Acanthamoeba
Tryptophan synthaseTryptophan synthase
"Gene Discovery in the Acanthamoeba castellanii Genome". Protist. 156 (2): 203-14. doi:10.1016/j.protis.2005.04.001. PMID ...
https://en.wikipedia.org/wiki/Tryptophan_synthase
5S ribosomal RNA5S ribosomal RNA
Bullerwell, CE; Schnare, MN; Gray, MW (March 2003). "Discovery and characterization of Acanthamoeba castellanii mitochondrial ...
https://en.wikipedia.org/wiki/5S_ribosomal_RNA
Aldo CastellaniAldo Castellani
The human pathogen Acanthamoeba castellanii is named after him. Manual of tropical medicine, 1910 (with Albert John Chalmers) ...
https://en.wikipedia.org/wiki/Aldo_Castellani
RNA editingRNA editing
Lonergan KM, Gray MW (September 1993). "Predicted editing of additional transfer RNAs in Acanthamoeba castellanii mitochondria ... insertion via the interaction between the gRNA and mRNA is similar to the tRNA editing processes in the animal and Acanthamoeba ...
https://en.wikipedia.org/wiki/RNA_editing
TupanvirusTupanvirus
... this phenomenon is also present when infecting host amoeba Acanthamoeba castellanii. Although more research is required it is ... Members of the family Mimiviridae includes Acanthamoeba polyphaga mimivirus (APMV) that is known for its large size of ~500 nm ...
https://en.wikipedia.org/wiki/Tupanvirus
Melanie BlokeschMelanie Blokesch
"An intracellular replication niche for Vibrio cholerae in the amoeba Acanthamoeba castellanii". The ISME Journal. 10 (4): 897- ...
https://en.wikipedia.org/wiki/Melanie_Blokesch
Andrew J. RogerAndrew J. Roger
"Evidence for a Hydrogenosomal-Type Anaerobic ATP Generation Pathway in Acanthamoeba castellanii". PLOS ONE. 8 (9): e69532. ...
https://en.wikipedia.org/wiki/Andrew_J._Roger
MyosinMyosin
Pollard TD, Korn ED (July 1973). "Acanthamoeba myosin. I. Isolation from Acanthamoeba castellanii of an enzyme similar to ... Following the discovery in 1973 of enzymes with myosin-like function in Acanthamoeba castellanii, a global range of divergent ...
https://en.wikipedia.org/wiki/Myosin
VirophageVirophage
It was discovered with its co-infecting giant virus, Acanthamoeba castellanii mamavirus (ACMV). The virophage was named Sputnik ...
https://en.wikipedia.org/wiki/Virophage
Marine virusesMarine viruses
It was discovered with its co-infecting giant virus, Acanthamoeba castellanii mamavirus (ACMV). The virophage was named Sputnik ...
https://en.wikipedia.org/wiki/Marine_viruses
Myosin-heavy-chain kinaseMyosin-heavy-chain kinase
"Purification and characterization of a myosin I heavy chain kinase from Acanthamoeba castellanii". J. Biol. Chem. 258 (16): ... Brzeska H, Lynch TJ, Martin B, Corigliano-Murphy A, Korn ED (1990). "Substrate specificity of Acanthamoeba myosin I heavy chain ... mutating the regulatory phosphoserine and conserved threonine on the activity of the expressed catalytic domain of Acanthamoeba ...
https://en.wikipedia.org/wiki/Myosin-heavy-chain_kinase
PARP3PARP3
"Oscillations of redox states in synchronously dividing cultures of Acanthamoeba castellanii and Schizosaccharomyces pombe". ...
https://en.wikipedia.org/wiki/PARP3
PARP2PARP2
"Oscillations of redox states in synchronously dividing cultures of Acanthamoeba castellanii and Schizosaccharomyces pombe". ...
https://en.wikipedia.org/wiki/PARP2
Contact lensContact lens
"In vitro effects of selected contact lens care solutions on Acanthamoeba castellanii strains in Poland". Experimental ... Poor lens care can lead to infections by various microorganisms including bacteria, fungi, and Acanthamoeba (Acanthamoeba ... "Comparative in vitro effectiveness of a novel contact lens multipurpose solution on Acanthamoeba castellanii". Journal of ... Gray T.B.; Cursons R.T.; Sherwan J.F.; Rose P.R. (1995). "Acanthamoeba, bacterial, and fungal contamination of contact lens ...
https://en.wikipedia.org/wiki/Contact_lens
VirusVirus
In similar manner, the sputnik virophage is dependent on mimivirus, which infects the protozoan Acanthamoeba castellanii. These ...
https://en.wikipedia.org/wiki/Virus
AmoebaAmoeba
The currently generally utilized and best-explored amoebae that host other organisms are Acanthamoeba castellanii and ... Acanthamoeba can cause amoebic keratitis and encephalitis in humans. Balamuthia mandrillaris is the cause of (often fatal) ... "Acanthamoeba , Microworld". www.arcella.nl. Archived from the original on 18 August 2016. Retrieved 21 August 2016. "Microscopy ... This finding suggests that the ''Acanthamoeba'' are capable of some form of meiosis and may be able to undergo sexual ...
https://en.wikipedia.org/wiki/Amoeba
MobilomeMobilome
Discovered in 2008 in a strain of Acanthamoeba castellanii mimivirus, virophages are an element of the virus mobilome. ...
https://en.wikipedia.org/wiki/Mobilome
MamavirusMamavirus
It was originally isolated from Acanthamoeba polyphaga, but subsequent work has involved Acanthamoeba castellanii (Acanthamoeba ... June 2011). "Viruses with more than 1000 genes: Mamavirus, a new Acanthamoeba castellanii mimivirus strain, and reannotation of ... Sputnik cannot replicate in acanthamoeba cells without a simultaneous infection by mamavirus (or mimivirus) so it infects the ... castellanii mamavirus, ACMV). Mamavirus, like other mimiviridae, is icosahedral with a core capsid and a peripheral fiber layer ...
https://en.wikipedia.org/wiki/Mamavirus
MedusavirusMedusavirus
The virus can harden amoebas of the species Acanthamoeba castellanii into stone-like cysts, but infection usually causes ...
https://en.wikipedia.org/wiki/Medusavirus
Legionella pneumophilaLegionella pneumophila
... pneumophila in Acanthamoeba castellanii, its natural host. L. pneumophila exhibits a biphasic lifecycle and defines ... In nature, L. pneumophila infects freshwater and soil amoebae of the genera Acanthamoeba and Naegleria. The mechanism of ... especially species of the genera Acanthamoeba and Naegleria, which can thus serve as a reservoir for L. pneumophila. These ...
https://en.wikipedia.org/wiki/Legionella_pneumophila
Spizellomyces punctatusSpizellomyces punctatus
... a rare modification that is only known to also exist in the Amoebozoa species Acanthamoeba castellanii and Chytridiomycota ... The patterns of mitochondrial 5' tRNA editing are similar to those found in A. castellanii. Ethylene and cytokinin receptors in ...
https://en.wikipedia.org/wiki/Spizellomyces_punctatus
ACMVACMV
Acanthamoeba castellanii mamavirus (ACMV) This disambiguation page lists articles associated with the title ACMV. If an ...
https://en.wikipedia.org/wiki/ACMV
MegavirusMegavirus
... was isolated by co-cultivation with a variety of Acanthamoeba laboratory strains (A. polyphaga, A. castellanii, A. ... phylogenetically related to Acanthamoeba polyphaga Mimivirus (APMV). In colloquial speech, Megavirus chilensis is more commonly ...
https://en.wikipedia.org/wiki/Megavirus
List of MeSH codes (B01)List of MeSH codes (B01)
... acanthamoeba MeSH B01.500.841.750.656.475.100.075.080.150 - acanthamoeba castellanii MeSH B01.500.841.750.656.475.100.200 - ...
https://en.wikipedia.org/wiki/List_of_MeSH_codes_(B01)
Muscarinic acetylcholine receptor M1Muscarinic acetylcholine receptor M1
A structural but not sequential homolog of the human M1 receptor has been reported in Acanthamoeba castellanii and Naegleria ... featuring Acanthamoeba spp bioinformatics 3D-modelling and experimentations". Journal of Receptor and Signal Transduction ...
https://en.wikipedia.org/wiki/Muscarinic_acetylcholine_receptor_M1
Medical uses of silverMedical uses of silver
... of central nervous system infections caused by free-living amoebae such as Naegleria fowleri and Acanthamoeba castellanii, ...
https://en.wikipedia.org/wiki/Medical_uses_of_silver
2014 Ju-Jitsu World Championships2014 Ju-Jitsu World Championships
The 2014 Ju-Jitsu World Championship were the 12th edition of the Ju-Jitsu World Championships, and were held in Paris, France from November 28 to November 30, 2014. 28.11.2014 - Men's and Women's Fighting System, Men's and Women's Jiu-Jitsu (ne-waza), Men's Duo System - Classic 29.11.2014 - Men's and Women's Fighting System, Men's and Women's Jiu-Jitsu (ne-waza), Women's Duo System - Classic 30.11.2014 - Men's Jiu-Jitsu (ne-waza), Mixed Duo System - Classic, Team event Vincent MATCZAK (2014-09-30). "4TH INVITAION TO WORLD CHAMPIONSHIP 2014" (PDF). Retrieved 2019-11-28.[dead link] Online results Official results (PDF) Mixed team event results (PDF) (All articles with dead external links, Articles with dead external links from April 2022, Ju-Jitsu World Championships, 2014 in French sport ...
https://en.wikipedia.org/wiki/2014_Ju-Jitsu_World_Championships

No data available that match "acanthamoeba castellanii"


KeratitisTrophozoitesPolyphaga mimivirusGenusORGANISMSAmoebaNeffVectorsGranulomatousStrainSpeciesCharacterizationOrganismGeneticQuantitativeAmoebaeCornealEncefalitisBiocidesContact lensSpecimensBacteriaBiologyCommonlyPlasmaBottlesChronicLaboratoryPersonsEnvironmentalGrowthSimilarSolutionsPositiveActivityReference
Keratitis13
  • In the past decade there has been an increased incidence of Acanthamoeba keratitis, particularly in contact lens wearers. (northumbria.ac.uk)
  • Twenty-four patients with Acanthamoeba keratitis have been reported to CDC from 14 states in the last 9 months (Table 1). (cdc.gov)
  • Acanthamoeba keratitis was diagnosed by examination of stained corneal scrapings or tissues (67%) and/or tissue indirect fluorescent antibody (IFA) test (52%) using species-specific antisera. (cdc.gov)
  • The first case of Acanthamoeba keratitis in the United States was reported in 1973 in a South Texas rancher with a history of trauma to his right eye (1). (cdc.gov)
  • The 24 Acanthamoeba keratitis cases described here represent a striking increase over those reported in previous years. (cdc.gov)
  • The antiamoebic effects of BAC, povidone iodine, and tetracaine are superior to the current diamidines and slightly inferior to the biguanides used in the treatment for Acanthamoeba keratitis. (arvojournals.org)
  • Currently, there are approximately 4.1 million CL wearers in the United Kingdom, 2 and established independent risk factors for developing acanthamoeba keratitis (AK) in CL wearers include exposure to tap water in the home, 3 , 4 swimming or bathing when wearing CL, 4 , 5 poor lens hygiene, 4 - 6 and the use of rigid CLs in orthokeratology. (arvojournals.org)
  • Acanthamoeba species are widely distributed free-living amoebae showing an increased role as human pathogens causing encephalitis, keratitis, pneumonitis and dermatitis. (lu.se)
  • Promethazine and its structural analogs, because of being FDA-approved, have a wider margin of safety that can be tested as potential anti- Acanthamoeba agents in diseases like keratitis and encephalitis caused by this protist pathogen. (aku.edu)
  • The genus Acanthamoeba is an amphizoic amoeba, which is a causative agent of vision-threatening keratitis and life-threatening granulomatous encephalitis ( Khan, 2006 ). (parasitol.kr)
  • Acanthamoeba castellanii is a ubiquitous, soil-dwelling protozoa responsible for Acanthamoebic keratitis of the eye, predominantly caused by improper contact lens disinfection. (gsu.edu)
  • infections (excluding keratitis) are defined as the detection of Acanthamoeba spp. (cdc.gov)
  • Acanthamoeba keratitis is a local infection of the cornea (outer layer of the visual pathway of the eye) caused by a microscopic, free-living ameba belonging to the genus Acanthamoeba . (cdc.gov)
Trophozoites11
  • The aim of this study was to utilize in vitro killing assays and to establish a novel, time-lapse, live-cell imaging methodology to demonstrate the efficacy of contact lens care solutions in eradicating Acanthamoeba castellanii (A. castellanii) trophozoites and cysts. (northumbria.ac.uk)
  • To compare the antimicrobial effect of topical anesthetics, antivirals, antibiotics, and biocides on the viability of Acanthamoeba cysts and trophozoites in vitro. (arvojournals.org)
  • Amoebicidal and cysticidal assays were performed against both trophozoites and cysts of Acanthamoeba castellanii (ATCC 50370) and Acanthamoeba polyphaga (ATCC 30461). (arvojournals.org)
  • Proxymetacaine, oxybuprocaine, and especially tetracaine were all toxic to the trophozoites and cysts of Acanthamoeba spp. (arvojournals.org)
  • We selected promethazine, which is a known antagonist of proteins like dopaminergic, histaminergic, muscarinic receptors, and calmodulin, to determine its effects on the growth and proliferation of trophozoites and cysts of Acanthamoeba spp. (aku.edu)
  • In order to elucidate the receptors involved in the effects produced by promethazine, we also performed individual experiments on Acanthamoeba trophozoites and cysts in the presence of the agonist of the above-mentioned receptors. (aku.edu)
  • Our results show that promethazine in the range of 60-100 μg/mL proved to be amoebicidal for Acanthamoeba trophozoites and at slightly higher doses ranging around 125-250 μg/mL also showed partial cysticidal effects. (aku.edu)
  • Trasnmission electron microscopy showed that the bacteria were randomly distributed within the cytoplasm of trophozoites and cysts of Acanthamoeba . (parasitol.kr)
  • Fields, 1996 ), Mycobacterium would multiply within Acanthamoeba trophozoites and cysts. (parasitol.kr)
  • as well as investigating methods for accurately counting Acanthamoeba trophozoites and cysts using flow cytometry. (gsu.edu)
  • A clinically compatible illness with positive identification of Acanthamoeba trophozoites or cysts using confocal microscopy. (cdc.gov)
Polyphaga mimivirus3
  • Based on a phylogenetic analysis, SMBV belongs to group A of the putative Megavirales order, possibly a new virus related to Acanthamoeba polyphaga mimivirus (APMV). (biomedcentral.com)
  • The discovery of the Acanthamoeba polyphaga mimivirus (APMV), arguably the most elusive member of the nucleocytoplasmic large DNA virus (NCLDV) group and the first discovered member of the Mimiviridae family, revived discussions regarding the evolution and origin of the viruses, as well as the differentiation between viruses and living organisms [ 1 ]. (biomedcentral.com)
  • Preliminary studies found Acanthamoeba polyphaga mimivirus (APMV) genomes in samples of bovine serum from Germany ( 13 , 14 ), indicating that the analysis of samples from vertebrates could be a way to explore and understand the circulation of this group of viruses in nature. (cdc.gov)
Genus4
  • Editorial Note: Members of the genus Acanthamoeba are the most common free-living amoebae in fresh water and soil. (cdc.gov)
  • Acanthamoeba is a genus of small, free-living amoebae common to most soil and freshwater habitats. (arvojournals.org)
  • Acanthamoeba is a genus of amoeboid protists in the family Acanthamoebidae . (eol.org)
  • The genus Acanthamoeba includes several species of opportunistic free-living amebae that might invade the brain through the blood, probably from a primary infection in the skin (from ulcers or dermatitis) or sinuses. (cdc.gov)
ORGANISMS4
  • Acanthamoebae have also been recovered from the nose and throat of humans with impaired respiratory function and from apparently healthy persons, suggesting that these organisms are commonly inhaled (1). (cdc.gov)
  • Organisms were exposed to serial two-fold dilutions of the test compounds in the wells of a microtiter plate to examine the effect on Acanthamoeba spp. (arvojournals.org)
  • Acanthamoeba organisms are ubiquitous in nature and can be found in bodies of water (e.g., lakes and oceans), soil, and air. (cdc.gov)
  • A standard inoculum of the ocular isolates of Staphylococcus aureus, Pseudomonas aeruginosa, Serratia marcescens, Aspergillus fumigatus, Fusarium solani, and Acanthamoeba castellanii were used as challenge organisms. (who.int)
Amoeba3
  • The existing knowledge of the L. pneumophila infection cycle in both hosts is summarized at the molecular level and the factors involved within amoeba and macrophages are compared and discussed in the light of recent findings from the Acanthamoeba castellanii genome analyses suggesting the existence of a primitive immune-like system in amoEBa. (semanticscholar.org)
  • The bacterium multiplied within Acanthamoeba , but exerted no cytopathic effects on the amoeba during a 6-year amoebic culture. (parasitol.kr)
  • Asexual reproduction in Amoeba castellanii is the process of growth by which a new individual is created from an existing cell without fertilization or genetic recombination, known as mitosis. (ibiologia.com)
Neff1
  • The fine structure of Acanthamoeba castellanii (Neff strain). (wikidata.org)
Vectors1
  • Wu D, Feng M1, Wang ZX, Qiao K, Tachibana H, Cheng XJ (2018) Molecular and biochemical characterization of key enzymes in the cysteine and serine metabolic pathways of Acanthamoeba castellanii Parasit Vectors 11 604. (unipr.it)
Granulomatous2
  • 1, 2 In the central nervous system (CNS), two main, well defined disease entities have been described: primary amoebic meningoencephalitis, which is caused by Naegleria fowlery and is rapidly fatal, and granulomatous amoebic encephalitis, which is caused by Acanthamoeba spp and Balamuthia mandrillaris and is characterised by focal granulomatous lesions in the brain following a subacute or chronic course. (bmj.com)
  • We diagnosed a fatal case of granulomatous amoebic encephalitis caused by Acanthamoeba castellanii by direct microscopy of a cerebrospinal fluid sample (CSF), Acanthamoeba cultivation, Giemsa staining, polymerase chain reaction and sequencing. (lu.se)
Strain2
  • The effects of Src kinase in the ability of A. castellanii to uptake live non-invasive E. coli strain, HB101. (biomedcentral.com)
  • We grew a large amount of strain KNic by using Acanthamoeba castellanii, which enabled phenotypic, genetic and phylogenetic analyses that supported its affiliation as Protochlamydia naegleriophila . (cdc.gov)
Species3
  • The species of Acanthamoeba was not determined for six (25%) patients. (cdc.gov)
  • More than one species of Acanthamoeba was cultured from samples from four patients. (cdc.gov)
  • Several species of Acanthamoeba are associated with infection (i.e. (cdc.gov)
Characterization1
  • In the present study, we found an endosymbiotic bacterium of Acanthamoeba during the characterization of environmental Acanthamoeba isolates from various origins via mitochondrial (Mt) DNA RFLP. (parasitol.kr)
Organism1
  • A negative test on CSF does not rule out Acanthamoeba infection because the organism is not commonly present in the CSF. (cdc.gov)
Genetic1
  • Identification of Atg8 from Acanthamoeba castellanii by genetic complementation in Saccharomyces cerevisiae. (univ-poitiers.fr)
Quantitative1
  • A) Growth rate of Protochlamydia naegleriophila within Acanthamoeba castellanii assessed using a specific quantitative real-time PCR. (cdc.gov)
Amoebae2
  • Amoebae are prominent grazers, with Acanthamoeba castellanii being one of the best-studied aquatic amoebae. (unamur.be)
  • Both bacteria were isolated from amoebae and were described as appearing within the nuclei of Acanthamoeba polyphaga and ultimately lysing their host cells within 4 days. (biomedcentral.com)
Corneal1
  • Acanthamoebae were isolated from the corneal scrapings/biopsies of 17 (71%) of the patients. (cdc.gov)
Encefalitis2
  • La Encefalitis granulomatosa amebiana es causada por la Acanthamoeba castellanii. (moroccanslots.com)
  • Puede causar ENCEFALITIS y QUERATITIS en seres humanos. (bvsalud.org)
Biocides1
  • These observations are in keeping with care solution biocides having prominent activity at the plasma membrane of Acanthamoeba. (northumbria.ac.uk)
Contact lens2
  • The Mt DNA RFLP pattern of Acanthamoeba KA/LC6 isolated from contact lens storage case evidenced several extra bands, considering the general size of the Mt DNA of Acanthamoeba . (parasitol.kr)
  • Acanthamoeba KA/LC6 was isolated from a contact lens storage case in Korea. (parasitol.kr)
Specimens1
  • Known occurrences, collected specimens and observations of Acanthamoeba. (eol.org)
Bacteria2
  • However, the artificially-induced transient association between the bacterial and amoebic hosts has made any detailed research into the mechanisms underlying the interaction between the endosymbiotic bacteria and Acanthamoeba difficult. (parasitol.kr)
  • Acanthamoeba" reproduce asexually through binary fission, similar to the process by which bacteria produce. (ibiologia.com)
Biology1
  • The significance of the reported receptors and ion channels in the biology of Acanthamoeba is yet to be determined. (aku.edu)
Commonly1
  • Acanthamoeba and B. mandrillaris can cause clinically similar illnesses and might be difficult to differentiate using commonly available laboratory procedures. (cdc.gov)
Plasma1
  • Plasma and phagosome membranes of Acanthamoeba castellanii. (wikidata.org)
Bottles1
  • Bacterial contamination was detected in 20 (57.1%) solution bottles and none yielded fungus or Acanthamoeba. (who.int)
Chronic1
  • Acanthamoeba GAE has a slow and insidious onset and develops into a subacute or chronic disease lasting several weeks to months. (cdc.gov)
Laboratory1
  • Laboratory-confirmed Acanthamoeba spp. (cdc.gov)
Persons1
  • Acanthamoeba GAE affects both immunocompetent persons and persons who are immunosuppressed from a variety of causes (e.g. (cdc.gov)
Environmental1
  • Acanthamoeba has been suspected as an environmental host of Mycobacterium sp. (parasitol.kr)
Growth3
  • KNic growth in A. castellanii was assessed by immunofluorescence with in-house mouse anti-KNic and Alexa488-coupled anti-immunoglobulin antibodies (Invitrogen, Eugene, OR, USA). (cdc.gov)
  • Zeiss, Feldbach, Switzerland) confirmed the intracellular location of KNic and demonstrated its rapid growth within A. castellanii . (cdc.gov)
  • To precisely assess the growth rate, we performed PCR on A. castellanii /KNic coculture by using PrF/PrR primers and PrS probe. (cdc.gov)
Similar1
  • We also show the evidence of homology between the human targets of promethazine and similar targets in Acanthamoeba by the use of bioinformatic computational tools and 3D modeling. (aku.edu)
Solutions1
  • All solutions were adequately effective against Acanthamoeba. (who.int)
Positive2
  • all were positive for Acanthamoeba. (cdc.gov)
  • Initial symptoms of Acanthamoeba GAE might include headache, photophobia, and stiff neck accompanied by positive Kernig's and Brudzinski's signs. (cdc.gov)
Activity1
  • With the diamidines, the presence of BAC in the propamidine drops was responsible for the activity against Acanthamoeba spp. (arvojournals.org)
Reference1
  • Two strains, A. castellanii Castellani and A. lugdunensis L3a, were employed as reference strains. (parasitol.kr)
Parachlamydiaceae: Potential Emerging Pathogens - Volume 8, Number 6-June 2002 - Emerging Infectious Diseases journal - CDC
Parachlamydiaceae: Potential Emerging Pathogens - Volume 8, Number 6-June 2002 - Emerging Infectious Diseases journal - CDC (wwwnc.cdc.gov)
Amoebae-resisting Bacteria Isolated from Human Nasal Swabs by Amoebal Coculture - Volume 10, Number 3-March 2004 - Emerging...
Amoebae-resisting Bacteria Isolated from Human Nasal Swabs by Amoebal Coculture - Volume 10, Number 3-March 2004 - Emerging... (wwwnc.cdc.gov)
Figure 1 - Protochlamydia naegleriophila as Etiologic Agent of Pneumonia - Volume 14, Number 1-January 2008 - Emerging...
Figure 1 - Protochlamydia naegleriophila as Etiologic Agent of Pneumonia - Volume 14, Number 1-January 2008 - Emerging... (wwwnc.cdc.gov)
Successful Treatment of Disseminated Acanthamoeba sp. Infection with Miltefosine - Volume 14, Number 11-November 2008 -...
Successful Treatment of Disseminated Acanthamoeba sp. Infection with Miltefosine - Volume 14, Number 11-November 2008 -... (wwwnc.cdc.gov)
Lectin Affinity Chromatography | Semantic Scholar
Lectin Affinity Chromatography | Semantic Scholar (semanticscholar.org)
Appendix Figure - Protochlamydia naegleriophila as Etiologic Agent of Pneumonia - Volume 14, Number 1-January 2008 - Emerging...
Appendix Figure - Protochlamydia naegleriophila as Etiologic Agent of Pneumonia - Volume 14, Number 1-January 2008 - Emerging... (wwwnc.cdc.gov)
Protochlamydia naegleriophila as Etiologic Agent of Pneumonia - Volume 14, Number 1-January 2008 - Emerging Infectious Diseases...
Protochlamydia naegleriophila as Etiologic Agent of Pneumonia - Volume 14, Number 1-January 2008 - Emerging Infectious Diseases... (wwwnc.cdc.gov)
Samba virus: a novel mimivirus from a giant rain forest, the Brazilian Amazon | Virology Journal | Full Text
Samba virus: a novel mimivirus from a giant rain forest, the Brazilian Amazon | Virology Journal | Full Text (virologyj.biomedcentral.com)
Parachlamydia spp. and Related Chlamydia-like Organisms and Bovine Abortion - Volume 13, Number 12-December 2007 - Emerging...
Parachlamydia spp. and Related Chlamydia-like Organisms and Bovine Abortion - Volume 13, Number 12-December 2007 - Emerging... (wwwnc.cdc.gov)
WHO EMRO | Identification of immunoreactive proteins in secretions of Leishmania infantum promastigotes: an immunoproteomic...
WHO EMRO | Identification of immunoreactive proteins in secretions of Leishmania infantum promastigotes: an immunoproteomic... (emro.who.int)
The role of Src kinase in the biology and pathogenesis of Acanthamoeba castellanii | Parasites & Vectors | Full Text
The role of Src kinase in the biology and pathogenesis of Acanthamoeba castellanii | Parasites & Vectors | Full Text (parasitesandvectors.biomedcentral.com)
Legionnaires' Disease at a Dutch Flower Show: Prognostic Factors and Impact of Therapy - Volume 8, Number 12-December 2002 -...
Legionnaires' Disease at a Dutch Flower Show: Prognostic Factors and Impact of Therapy - Volume 8, Number 12-December 2002 -... (wwwnc.cdc.gov)
Find Research outputs - Discovery - the University of Dundee Research Portal
Find Research outputs - Discovery - the University of Dundee Research Portal (discovery.dundee.ac.uk)
Pathogen & Environment | Acanthamoeba | Parasites | CDC
Pathogen & Environment | Acanthamoeba | Parasites | CDC (cdc.gov)
Common ancestry of heterodimerizing TALE homeobox transcription factors across Metazoa and Archaeplastida | BMC Biology | Full...
Common ancestry of heterodimerizing TALE homeobox transcription factors across Metazoa and Archaeplastida | BMC Biology | Full... (bmcbiol.biomedcentral.com)
Publications list | The James Hutton Institute
Publications list | The James Hutton Institute (hutton.ac.uk)
CDC - DPDx - Free Living Amebic Infections
CDC - DPDx - Free Living Amebic Infections (cdc.gov)
Cyril Noel
Cyril Noel (annuaire.ifremer.fr)
Fondation Recherches 3R / Projets / 3R-Project 99-05
Fondation Recherches 3R / Projets / 3R-Project 99-05 (forschung3r.ch)
Identification of amoebae in the CSF in a patient with meningoencephalitis | Journal of Neurology, Neurosurgery & Psychiatry
Identification of amoebae in the CSF in a patient with meningoencephalitis | Journal of Neurology, Neurosurgery & Psychiatry (jnnp.bmj.com)
In vitro activity of 1H-phenalen-1-one derivatives against Acanthamoeba castellanii Neff and their mechanisms of cell death |...
In vitro activity of 1H-phenalen-1-one derivatives against Acanthamoeba castellanii Neff and their mechanisms of cell death |... (portalciencia.ull.es)
Molecular insights into Vibrio cholerae's intra-amoebal host-pathogen interactions<...
Molecular insights into Vibrio cholerae's intra-amoebal host-pathogen interactions<... (researchportal.unamur.be)
SWISS-MODEL Template Library | 1f2k
SWISS-MODEL Template Library | 1f2k (swissmodel.expasy.org)
Microbial Adhesion to Silicone Hydrogel Lenses
Microbial Adhesion to Silicone Hydrogel Lenses (medscape.com)
Medscape | Eye Contact Lens - Publication Information
Medscape | Eye Contact Lens - Publication Information (medscape.com)