Clusters of colonic crypts that appear different from the surrounding mucosa when visualized after staining. They are of interest as putative precursors to colorectal adenomas and potential biomarkers for colorectal carcinoma.
A potent carcinogen and neurotoxic compound. It is particularly effective in inducing colon carcinomas.
Pathological processes that tend eventually to become malignant. (From Dorland, 27th ed)
A DNA alkylating agent that has been shown to be a potent carcinogen and is widely used to induce colon tumors in experimental animals.
Tumors or cancer of the COLON.
Substances that increase the risk of NEOPLASMS in humans or animals. Both genotoxic chemicals, which affect DNA directly, and nongenotoxic chemicals, which induce neoplasms by other mechanism, are included.
The segment of LARGE INTESTINE between the CECUM and the RECTUM. It includes the ASCENDING COLON; the TRANSVERSE COLON; the DESCENDING COLON; and the SIGMOID COLON.
Hydrazines substituted with two methyl groups in any position.
Agents that reduce the frequency or rate of spontaneous or induced tumors independently of the mechanism involved.
Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.
A subcategory of mucins that contain SIALIC ACID.
Pathological processes in the COLON region of the large intestine (INTESTINE, LARGE).
A benign epithelial tumor with a glandular organization.
A plant genus of the family ZINGIBERACEAE, order Zingiberales, subclass Zingiberidae. Elettaria cardamomum (L.) Maton is the source of Cardamom used in SPICES.
The period of history from 1451 through 1600 of the common era.
Tumors or cancer of the COLON or the RECTUM or both. Risk factors for colorectal cancer include chronic ULCERATIVE COLITIS; FAMILIAL POLYPOSIS COLI; exposure to ASBESTOS; and irradiation of the CERVIX UTERI.
Tumor suppressor genes located in the 5q21 region on the long arm of human chromosome 5. The mutation of these genes is associated with familial adenomatous polyposis (ADENOMATOUS POLYPOSIS COLI) and GARDNER SYNDROME, as well as some sporadic colorectal cancers.
The chromosome region which is active in nucleolus formation and which functions in the synthesis of ribosomal RNA.
The goosefoot plant family of the order Caryophyllales, subclass Caryophyllidae, class Magnoliopsida. It includes beets and chard (BETA VULGARIS), as well as SPINACH, and salt tolerant plants.
Tumors or cancer of the INTESTINES.
A plant genus of the family ARACEAE. Members contain konjac glucomannan (MANNANS) and SEROTONIN.
Thirteen-carbon butene cyclohexene degradation products formed by the cleavage of CAROTENOIDS. They contribute to the flavor of some FRUIT. Ionone should not be confused with the similarly named ionol.
The combination of two or more different factors in the production of cancer.
A nonsteroidal anti-inflammatory agent with potent analgesic and antiarthritic properties. It has been shown to be effective in the treatment of OSTEOARTHRITIS; RHEUMATOID ARTHRITIS; ankylosing SPONDYLITIS; and in the alleviation of postoperative pain (PAIN, POSTOPERATIVE).
A starch found in the tubers and roots of many plants. Since it is hydrolyzable to FRUCTOSE, it is classified as a fructosan. It has been used in physiologic investigation for determination of the rate of glomerular function.
A sulfinylindene derivative prodrug whose sulfinyl moiety is converted in vivo to an active NSAID analgesic. Specifically, the prodrug is converted by liver enzymes to a sulfide which is excreted in the bile and then reabsorbed from the intestine. This helps to maintain constant blood levels with reduced gastrointestinal side effects.
A flavonol glycoside found in many plants, including BUCKWHEAT; TOBACCO; FORSYTHIA; HYDRANGEA; VIOLA, etc. It has been used therapeutically to decrease capillary fragility.
A plant genus of the family HIPPOCASTANACEAE (or SAPINDACEAE by some) that contains antimicrobial protein 1 and escin. A. hippocastanum is used in folk medicine for treating chronic venous insufficiency.
High molecular weight mucoproteins that protect the surface of EPITHELIAL CELLS by providing a barrier to particulate matter and microorganisms. Membrane-anchored mucins may have additional roles concerned with protein interactions at the cell surface.
Family of retrovirus-associated DNA sequences (ras) originally isolated from Harvey (H-ras, Ha-ras, rasH) and Kirsten (K-ras, Ki-ras, rasK) murine sarcoma viruses. Ras genes are widely conserved among animal species and sequences corresponding to both H-ras and K-ras genes have been detected in human, avian, murine, and non-vertebrate genomes. The closely related N-ras gene has been detected in human neuroblastoma and sarcoma cell lines. All genes of the family have a similar exon-intron structure and each encodes a p21 protein.
A multi-functional catenin that participates in CELL ADHESION and nuclear signaling. Beta catenin binds CADHERINS and helps link their cytoplasmic tails to the ACTIN in the CYTOSKELETON via ALPHA CATENIN. It also serves as a transcriptional co-activator and downstream component of WNT PROTEIN-mediated SIGNAL TRANSDUCTION PATHWAYS.
A cyclooxygenase inhibiting, non-steroidal anti-inflammatory agent (NSAID) that is well established in treating rheumatoid arthritis and osteoarthritis and used for musculoskeletal disorders, dysmenorrhea, and postoperative pain. Its long half-life enables it to be administered once daily.
The use of chemical compounds to prevent the development of a specific disease.
Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard.
Regular course of eating and drinking adopted by a person or animal.
A polyposis syndrome due to an autosomal dominant mutation of the APC genes (GENES, APC) on CHROMOSOME 5. The syndrome is characterized by the development of hundreds of ADENOMATOUS POLYPS in the COLON and RECTUM of affected individuals by early adulthood.
Discrete tissue masses that protrude into the lumen of the COLON. These POLYPS are connected to the wall of the colon either by a stalk, pedunculus, or by a broad base.
Discrete abnormal tissue masses that protrude into the lumen of the INTESTINE. A polyp is attached to the intestinal wall either by a stalk, pedunculus, or by a broad base.
A segment of the LOWER GASTROINTESTINAL TRACT that includes the CECUM; the COLON; and the RECTUM.
The blind sac or outpouching area of the LARGE INTESTINE that is below the entrance of the SMALL INTESTINE. It has a worm-like extension, the vermiform APPENDIX.
A compound consisting of dark green crystals or crystalline powder, having a bronze-like luster. Solutions in water or alcohol have a deep blue color. Methylene blue is used as a bacteriologic stain and as an indicator. It inhibits GUANYLATE CYCLASE, and has been used to treat cyanide poisoning and to lower levels of METHEMOGLOBIN.
The products of chemical reactions that result in the addition of extraneous chemical groups to DNA.
An inducibly-expressed subtype of prostaglandin-endoperoxide synthase. It plays an important role in many cellular processes and INFLAMMATION. It is the target of COX2 INHIBITORS.
The remnants of plant cell walls that are resistant to digestion by the alimentary enzymes of man. It comprises various polysaccharides and lignins.
The distal segment of the LARGE INTESTINE, between the SIGMOID COLON and the ANAL CANAL.
The mass or quantity of heaviness of an individual. It is expressed by units of pounds or kilograms.
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
A pyridoxal-phosphate protein, believed to be the rate-limiting compound in the biosynthesis of polyamines. It catalyzes the decarboxylation of ornithine to form putrescine, which is then linked to a propylamine moiety of decarboxylated S-adenosylmethionine to form spermidine.
Biphenyl compounds substituted in any position by one or more amino groups. Permitted are any substituents except fused rings.
The portion of the GASTROINTESTINAL TRACT between the PYLORUS of the STOMACH and the ILEOCECAL VALVE of the LARGE INTESTINE. It is divisible into three portions: the DUODENUM, the JEJUNUM, and the ILEUM.
Compounds containing 1,3-diazole, a five membered aromatic ring containing two nitrogen atoms separated by one of the carbons. Chemically reduced ones include IMIDAZOLINES and IMIDAZOLIDINES. Distinguish from 1,2-diazole (PYRAZOLES).
Endoscopic examination, therapy or surgery of the luminal surface of the colon.
A process involving chance used in therapeutic trials or other research endeavor for allocating experimental subjects, human or animal, between treatment and control groups, or among treatment groups. It may also apply to experiments on inanimate objects.
Long-chain polymer of glucose containing 17-20% sulfur. It has been used as an anticoagulant and also has been shown to inhibit the binding of HIV-1 to CD4-POSITIVE T-LYMPHOCYTES. It is commonly used as both an experimental and clinical laboratory reagent and has been investigated for use as an antiviral agent, in the treatment of hypolipidemia, and for the prevention of free radical damage, among other applications.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
A class of compounds composed of repeating 5-carbon units of HEMITERPENES.
A malignant epithelial tumor with a glandular organization.
An increase in the number of cells in a tissue or organ without tumor formation. It differs from HYPERTROPHY, which is an increase in bulk without an increase in the number of cells.
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
Methods of investigating the effectiveness of anticancer cytotoxic drugs and biologic inhibitors. These include in vitro cell-kill models and cytostatic dye exclusion tests as well as in vivo measurement of tumor growth parameters in laboratory animals.
A transferase that catalyzes the addition of aliphatic, aromatic, or heterocyclic FREE RADICALS as well as EPOXIDES and arene oxides to GLUTATHIONE. Addition takes place at the SULFUR. It also catalyzes the reduction of polyol nitrate by glutathione to polyol and nitrite.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Use of plants or herbs to treat diseases or to alleviate pain.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Anti-inflammatory agents that are non-steroidal in nature. In addition to anti-inflammatory actions, they have analgesic, antipyretic, and platelet-inhibitory actions.They act by blocking the synthesis of prostaglandins by inhibiting cyclooxygenase, which converts arachidonic acid to cyclic endoperoxides, precursors of prostaglandins. Inhibition of prostaglandin synthesis accounts for their analgesic, antipyretic, and platelet-inhibitory actions; other mechanisms may contribute to their anti-inflammatory effects.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
Enzyme complexes that catalyze the formation of PROSTAGLANDINS from the appropriate unsaturated FATTY ACIDS, molecular OXYGEN, and a reduced acceptor.
The consumption of edible substances.
A CALCIUM-independent subtype of nitric oxide synthase that may play a role in immune function. It is an inducible enzyme whose expression is transcriptionally regulated by a variety of CYTOKINES.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
A malignant neoplasm made up of epithelial cells tending to infiltrate the surrounding tissues and give rise to metastases. It is a histological type of neoplasm but is often wrongly used as a synonym for "cancer." (From Dorland, 27th ed)
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Naturally occurring or synthetic substances that inhibit or retard the oxidation of a substance to which it is added. They counteract the harmful and damaging effects of oxidation in animal tissues.
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
The growth of INTESTINAL POLYPS. Growth processes include neoplastic (ADENOMA and CARCINOMA) and non-neoplastic (hyperplastic, mucosal, inflammatory, and other polyps).
A mass of histologically normal tissue present in an abnormal location.
A method of data collection and a QUALITATIVE RESEARCH tool in which a small group of individuals are brought together and allowed to interact in a discussion of their opinions about topics, issues, or questions.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
A rod-shaped, gram-positive, non-acid-fast, non-spore-forming, non-motile bacterium that is a genus of the family Bifidobacteriaceae, order Bifidobacteriales, class ACTINOBACTERIA. It inhabits the intestines and feces of humans as well as the human vagina.
Elements of limited time intervals, contributing to particular results or situations.
Agents that reduce the frequency or rate of spontaneous or induced mutations independently of the mechanism involved.

Optimization of visibility and quantification of aberrant crypt foci in colonic mucosa in Wistar rats. (1/38)

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Enhanced induction of mucin-depleted foci in estrogen receptor {beta} knockout mice. (2/38)

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Toll-like receptor 2 signaling protects mice from tumor development in a mouse model of colitis-induced cancer. (3/38)

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Sodium butyrate does not decrease the evolution of precancerous lesions in rats. (4/38)

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Inhibition of 1, 2-dimethylhydrazine-induced mucin-depleted foci and O(6)-methylguanine DNA adducts in the rat colorectum by boiled garlic powder. (5/38)

The scavenging capacity of reactive oxygen species, such as hydroxyl radicals, is reported not to decrease in boiled garlic (an odorless garlic preparation). We therefore examined the modifying effect of boiled garlic powder (BGP) on 1,2-dimethylhydrazine-induced mucin-depleted foci (MDF) and aberrant crypt foci (ACF), preneoplastic lesions, in the rat colorectum. Male F344 rats (5 weeks old) were fed a basal diet, or experimental diets containing 5% or 1% BGP for 5 weeks. One week later, all rats were injected s.c. with DMH (40 mg/kg, once weekly for 2 weeks). At 10 weeks of age, all the rats were sacrificed, and the colorectum was evaluated for MDF and ACF. In rats given DMH and the 5% or 1% BGP diets (Groups 2 and 3), the numbers of MDF decreased significantly in a dose-dependent manner, compared with the DMH and basal diet value (Group 1) (p<0.01). The numbers of ACF in Group 2, but not Group 3, showed a non-significant tendency to decrease. Next, the effects of BGP on the formation of DMH-induced O6-methylguanine (O6-MeG) DNA adducts in rats were studied. Male F344 rats (5 weeks old) were fed the basal diet, or 10% BGP diet for 5 weeks. All rats were injected i.p. once with 40 mg/kg DMH at the end of week 5. The animals were sacrificed 6 hours after DMH injection to analyze the O6-MeG DNA adducts in the colorectal mucosa. Dietary administration of BGP significantly inhibited the O6-MeG DNA adduct levels in the colorectal mucosa, compared with the controls (p<0.01). These results suggested that BGP may exert chemopreventive effects against colon carcinogenesis at least in the initiation stage.  (+info)

Randomized phase II trial of sulindac, atorvastatin, and prebiotic dietary fiber for colorectal cancer chemoprevention. (6/38)

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Histomorphology of aberrant crypt foci in colorectal carcinoma. (7/38)

Colorectal carcinogenesis is a complex multistep process that includes changes in histomorphological appearance of the colonic mucosa and changes at molecular level. Aberrant crypt foci (ACF) was first described by Bird in 1987 on examination of methylene-blue-stained colonic mucosa of azoxymethane-treated mice under light microscopy. Since then ACF was considered as the earliest preneoplastic change that can be seen in the colonic mucosa. The aim of this study was to look at the histomorphology and distribution of ACF in colorectal carcinoma. 50 formalin-fixed archival colectomy specimens for colorectal carcinoma were examined under light microscopy after staining with 0.2% methylene blue. ACF was identified by larger and darker crypts with thickened epithelium, and often elevated from adjacent normal mucosa. ACF was found in 41 of 50 colectomy specimens examined. There were 328 ACF consisting of 36 (11.0%) ACF without hyperplasia or dysplasia, 263 (80.2%) ACF with hyperplasia and 29 (8.8%) ACF with dysplasia. Of these 29 ACF with dysplasia, 25 showed low grade dysplasia and four high grade dysplasia. The density of ACF was higher in the left colon, those older than 65 years of age and among males but these findings were statistically not significant. The crypt multiplicity of hyperplastic ACF (30.149, SD 28.395) was larger than dysplastic ACF (20.613, SD 40.128). The spectrum of histological changes observed probably represent the evolution of ACF in colorectal carcinogenesis.  (+info)

Randomized double-blind trial of sulindac and etodolac to eradicate aberrant crypt foci and to prevent sporadic colorectal polyps. (8/38)

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Aberrant crypt foci (ACF) are one of the earliest histopathological manifestations of colon cancer. In this review, we critically present the molecular, cellular, histopathological, and chemopreventive evidence that ACF are relevant biomarkers for colon cancer. The laboratory and clinical evidence are highly suggestive that ACF are in the pathway leading to colon cancer, but not all ACF will do so. The possible fate and outcome of ACF in the progression toward colon cancer may be dependent on a number of features that define their predictive power for the prevention or progression of cancer.
Aberrant crypt foci can be identified in the colons of rodents treated 3 wk earlier with azoxymethane, a known colon carcinogen. These crypts can easily be visualized in the unsectioned methylene blue-stained colons under light microscopy, where they are distinguished by their increased size, more prominent epithelial cells, and pericryptal space. They occur as single aberrant crypts or as two, three, or four aberrant crypts in a cluster. We compared the reported ability of carcinogens associated with the human diet to induce colon cancer with the measured rate of induction of aberrant crypts in female CF1 mice and Sprague-Dawley rats. The carcinogens used were 1,2-dimethylhydrazine, methyl nitrosourea, N-nitrosodimethylamine, benzo(a)pyrene, aflatoxin B1, 2-amino-6-methyl-dipyrido[1,2-α:3′,2′-d]imidazole, 2-amino-3-methylimidazo[4,5-P]quinoline, 2-amino-3,4-dimethylimidazo[4,5-P]quinoline, and 3-amino-1-methyl-5H-pyrido[4,3-b]indole. Graded doses of these compounds were given to the ...
Rice stem cell serum made from the best of Thai rice varieties is rich in antioxidants and essential amino acids that stimulate the growth of skin cells and slow the aging process.
The precise and systematic digestion and subsequent absorption of nutrients in food is vitally important for the life of all metazoans. The intestine is the organ primarily responsible for absorbing, processing, transporting, and excreting the vast variety of nutrients found in an organisms diet, and the tissue is maintained throughout its length by multipotent intestinal stem cells. Although previous work identified regions of preferential copper and iron absorption, only recent work is revealing the degree to which regions of the tissue can be specialized. The work presented in this thesis categorizes the extent of physiological, morphological, organizational and molecular heterogeneity along the intestinal anterior-posterior axis. This work is also the first to test stem cells of specific intestinal regions for their tumorigenic capacity and their ability to generate progeny of neighboring regions. I analyzed the properties of intestinal cells along the anterior-posterior axis and found that ...
Consuming higher amounts of calcium may lower the likelihood of precancerous colon and rectal lesions in people who are at increased risk due to variations in t
CHICAGO-Flat, non-polypoid colorectal neoplasms (NP-CRNs), which may be difficult to detect, appear to be relatively common and may have a greater association with cancer compared with the more routinely diagnosed type of colorectal polyps, according to a study in the March 5 issue of JAMA.
This project is supported by the Canadian Institutes of Health Research (award #111062), Alberta Innovates - Health Solutions, and by The Metabolomics Innovation Centre (TMIC), a nationally-funded research and core facility that supports a wide range of cutting-edge metabolomic studies. TMIC is funded by Genome Alberta, Genome British Columbia, and Genome Canada, a not-for-profit organization that is leading Canadas national genomics strategy with funding from the federal government. Maintenance, support, and commercial licensing is provided by OMx Personal Health Analytics, Inc. Designed by Educe Design & Innovation Inc. ...
Principles of Cell and Molecular Biology Study Area IV. Prereq.: BMS 102 or BIO 121; or permission of department chair. Introduction to the major principles of cell biology including cell compartmentalization; flow of genetic information; protein structure, synthesis, and trafficking; signal transduction; and molecular responses resulting in changes in cell activity, cell division, or apoptosis. Three hours of lecture and one, three-hour laboratory per week ...
A total of 73 CRC patients who underwent MICR met the inclusion criteria (34 males /39 female, mean ages 66.3± 12.8 years; 28% rectal and 62% colon lesions). The mean incision length was 7.8 ± 3.6cm and mean length of stay was 6.8± 4.4 days. The final cancer staging breakdown was; Stage I, 27%, Stage II, 33%, stage III, 36% and stage IV, 4%. The mean PreOp MMP3 level was 14.9±7.8 ng/ml (n=73). Significantly elevated mean plasma levels were noted on POD1 (21.4±14.7ng/ml, n= 73, p=, 0.0001), POD3 (37.9±21.5 ng/ml, n= 72, p=, 0.0001), POD7-13 (22.0±13.0 ng/ml, n=56, p=, 0.0001), POD14-20 (21.9±10.3 ng/ml, n=20, p=0.003) and on POD 21-27 (21.9±11.43 ng/ml, n= 20, p=0.002) when compared to PreOp levels. Plasma levels returned to the PreOp baseline at the POD 28-41 time point (n=16, p=0.07).. Conclusion ...
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Ingredient Treasury For Gluten-Free BakingFlaxseed Meal: Known for being high in Omega 3 Fatty Acids and fiber, flaxseed meal is a favorable Can you use just
Colorectal cancer is one of the leading causes of cancer death, both in men and women. This study investigatedthe effects of Amorphophallus campanulatus tuber methanolic extract (ACME) on aberrant crypt foci (ACF)formation, colonic cell proliferation, lipid peroxidative damage and the antioxidant status in a long term preclinicalmodel of 1, 2-dimethylhydrazine (DMH) induced colon carcinogenesis in rats. Male Wistar rats were dividedinto six groups, viz., group I rats served as controls; group II rats treated as drug controls receiving 250 mg/kg body weight of ACME orally; group III rats received DMH (20 mg/kg body weight) subcutaneously once aweek for the first 15 weeks; groups IV, V and VI rats received ACME along with DMH during the initiation,post- initiation stages and the entire period of the study, respectively. All the rats were sacrificed at the end of 30weeks and the intestinal and colonic tissues from different groups were subjected to biochemical and histologicalstudies. Administration of DMH
Processed meat intake is associated with colorectal cancer risk, but no experimental study supports the epidemiologic evidence. To study the effect of meat processing on carcinogenesis promotion, we first did a 14-day study with 16 models of cured meat. Studied factors, in a 2 × 2 × 2 × 2 design, were muscle color (a proxy for heme level), processing temperature, added nitrite, and packaging. Fischer 344 rats were fed these 16 diets, and we evaluated fecal and urinary fat oxidation and cytotoxicity, three biomarkers of heme-induced carcinogenesis promotion. A principal component analysis allowed for selection of four cured meats for inclusion into a promotion study. These selected diets were given for 100 days to rats pretreated with 1,2-dimethylhydrazine. Colons were scored for preneoplastic lesions: aberrant crypt foci (ACF) and mucin-depleted foci (MDF). Cured meat diets significantly increased the number of ACF/colon compared with a no-meat control diet (P = 0.002). Only the cooked ...
Effects Of Ground Flaxseed In Swine Diets On Pig Performance ...(CO) diet or a 15% flaxseed (FS) diet fed for 7, 14, 21, or 28 d prior to slaughter. Flaxseed
The nutritional value of flaxseed meal (FSM), a by-product of the flax crushing industry, has not been evaluated properly for use within swine rations. A series of experiments were conducted to determine the nutritional profile of this novel feed ingredient for pigs.The analysis of FSM revealed that it contains, on a dry matter (DM) basis, 133 g/kg ether extract (EE), 345 g/kg crude protein (CP), 60 g/kg ash, 164 g/kg ADF, 250 g/kg NDF, 102 g/kg crude fibre, 14 g/kg starch and 9 g/kg phosphorus. The gross energy (GE) content of the meal was 5.2 Mcal/kg DM. The ether extract fraction was characterized by, as a percent of total fat, 46.6% á-linolenic acid, an omega-3 fatty acid. Palmitic, stearic, oleic and linoleic acids accounted for 9.5, 4.8, 20.7 and 18.4% of the total fat content respectively. The crude protein content was well balanced for all amino acids with the exception of lysine (4.1% of CP), the level of which falls below that of the requirements for growing pigs (5.3% of CP for pigs ...
Background and aims: The molecular mechanisms underlying the promotion of colorectal carcinogenesis by a high-fat diet (HFD) remain unclear. We investigated the role of the insulin-signal pathway and the c-Jun N-terminal kinase (JNK) pathway, which reportedly play crucial roles in insulin resistance, during colorectal carcinogenesis in the presence of hyperinsulinemia induced by a HFD.. Methods: Azoxymethane-induced aberrant crypt foci formation and cell proliferation in the colonic epithelium were compared between mice fed a normal diet (ND) and mice fed a HFD. A western blot analysis was performed to elucidate the mechanism affecting colorectal carcinogenesis by a HFD.. Results: The number of aberrant crypt foci and the colonic epithelial cell proliferative activity were significantly higher in the HFD group than in the ND group. While the plasma insulin level was significantly higher in the HFD group than in the ND group, a western blot analysis revealed the inactivation of Akt, which is ...
Im looking for recipes for pumpkin bread/muffins/cookies/[whatever I can make with some canned pumpkin, Bisquick, raisins, cinnamon], and came across
Original entry: Nefeli Georgoulia, APPHY 226, Spring 2009 ==Overview== Authors: L. Mahadevan & T.J. Mitchinson Source: Nature, Vol. 435, 895-896, (2005) Soft Matter key words: polymerization, strain, mechanical bending ==Abstract== This paper is a collaboration between microtubule guru T. Mitchison and biotheorist giant L. Mahadevan. The two join forces to explore past work and to offer novel interpretation to the ever-tantalizing question: How is the chemical energy from GTP hydrolysis harnessed to power growth and shrinkage of microtubules in dynamic instability? Microtubules are part of the cellular cytoskeleton and their structure is that of a hollow tube, formed by the the stacking of thirteen protofilaments. They form a dynamic network through the continuous polymerization and depolymerization of tubulin monomer. This dynamic behavior regulates cell compartmentalization, cellular cargo transportation and perhaps most notably the tearing apart of chromosomes during cell ...
The study shows that one of the ECM proteolytic enzymes, the matrix metalloproteinase MMP9, protects mice that are also deficient for the protease plasmin from developing lethal inflammatory mass lesions in the colon. The lethal lesions develop from microscopic colon lesions caused by the inability of mice lacking functional plasmin to break down fibrin. MMP9 is not expressed in healthy colon mucosa but is delivered to the microscopic lesions in the colons of plasmin-deficient mice by invading inflammatory cells. However, in contrast to the combined lethal effects of MMP9 and plasmin deficiency during colon tissue homeostasis, male mice deficient for both MMP9 and plasmin do not differ from plasmin single-deficient mice in terms of incisional skin wound healing, implying that, under certain circumstances, other proteases are able to substitute for MMP9. ...
Maruti 800 Mdf Car Frame Oval - Mdf 1D Shv, Price of Maruti 800 Mdf Car Frame Oval - Mdf 1D Shv, Cost of Maruti 800 Mdf Car Frame Oval - Mdf 1D Shv accessory. See the Price, Image and Description of Maruti 800 car Mdf Car Frame Oval - Mdf 1D Shv.
The dose-response relationship in male F344 rats was determined for the ability of aspirin administered in the diet to prevent azoxymethane (AOM)-induced colon cancer and aberrant crypt foci (ACF) and to reduce prostaglandin E2 (PGE2) levels. Starting at either 7 or 22 weeks of age, the rats received aspirin. All rats received two doses of AOM (15 mg/kg each on days 7 and 14) and were killed on day 36. The lowest concentrations of aspirin to prevent ACF or reduce PGE2 levels were 600 and 400 mg/kg, respectively. To evaluate the prevention of tumors, rats received either 0 or 400 mg/kg aspirin for a total of 39 weeks with AOM (30 mg/kg) administered 7 days after the start of treatment. Aspirin had no effect on the yield of colon tumors. In a second experiment, rats started to receive 0, 200, 600 or 1800 mg/kg aspirin or 1000 mg/kg α-difluoromethylornithine (DFMO) +/- aspirin. Eight and 15 days later, all the rats received 15 mg/kg AOM. Eleven weeks later, animals that were receiving the control ...
The benefits of flaxseed are great in number. For a start of there are lots of good nutrients in each little seed. These include a wide spectrum of vitamins and minerals and lots of really good Omega 3 fats. In fact they have so much Omega 3 that they rival fish as a source of these valuable fats. This is good news if you are a vegan! More good news for vegans is that you can make a very good egg substitute using the gel found in flaxseeds. This gel is also found in the ground meal. It is very easy to use flaxseed meal as an egg substitute. You should also use it if you have an egg allergy.. Another neat thing about flaxseeds is that they contain practically zero amounts of carbohydrate. This is because the stored energy of the seed is in the form of oil. As a result they are great when it comes to producing food to fit in with a low carb diet.. You can use flaxseed meal in the same way that you use flour. This means that it is great for baking. Exchanging part of the flour for flaxseed meal ...
PRIMARY OBJECTIVES:. I. To determine mean percentage change from baseline in prostaglandin E2 (PGE2) within ACF pre and post 30 days of curcumin administration at a specified dose.. SECONDARY OBJECTIVS:. I. To determine mean percentage change from baseline in 5-hydroxy-eicosatetraenoic acid (5-HETE) within ACF pre and post 30 days of curcumin administration at a specified dose.. II. To determine mean percentage change from baseline in PGE2 and 5-HETE within comparison normal mucosa pre and post 30 days of curcumin administration at a specified dose.. III. To quantify corresponding enzyme changes in the cyclooxygenases (COX-1, COX-2,) and lipoxygenase (5-LOX) protein abundance. Semi-quantitative changes in these proteins will be measured by western blotting and correlated with changes in prostaglandins and leukotrienes respectively.. IV. Document changes in total ACF number. V. Determine proliferation by Ki-67 IHC in rectal mucosa pre and post therapy and correlate with changes in ACF number and ...
Follow This cooking class at Lets Get Cookin in Westlake Village was great fun - presented by prolific cookbook author, Hugh Carpenter, we foodies were immersed in Thai recipes, always good eats. Hughs dry sense of humor kept us in
Background Tertiary lymphoid structures (TLS) are leukocytes aggregates forming in non-lymphoid tissues in response to chronic inflammation. Salivary glands (SG) of patients with Sjögrens syndrome (SS) develop TLS characterised by B/T cell compartmentalization, specialised vasculature (HEV), functional B cell activation and local differentiation of autoreactive plasma cells. Although TLS presence in SS SG associates with an aggressive disease progression, the mechanisms triggering their formation in SS are largely unknown. We recently developed a model of inducible TLS formation, breach of self-tolerance and salivary hypofunction upon delivery of a replication-deficient adenovirus-5 (AdV5) into C57BL/6 mice SG. In this model, 3 weeks after infection, we observed fully formed and functional TLS, breach of self-tolerance and loss of salivary function.. Here we characterised the presence, function and relevance in TLS formation of the myeloid compartment in the first month post-infection. ...
Preventive intervention of colorectal cancer has become essential as a major portion of the population may develop the disease at some points during their lives. Diet and nutrition play an important role during this multistep colon carcinogenic process. Inhibitory activity of aqueous suspensions of garlic and tomato, individually and in combination, were tested on azoxymethane induced colon carcinogenesis in Sprague-Dawley rats. The effect was observed on aberrant crypt foci (ACF), the preneoplastic lesion. To investigate the mechanism of action of the agents used, cell proliferation and the level of apoptosis were determined and the expression of cyclooxygenase-2 (COX-2) protein was analyzed in the colon. Following treatment, significant inhibition of the level of cell proliferation (P|0.01 in garlic; P|0.001 in tomato and P|0.001 in combination treatment group with respect to the carcinogen control group), significant induction of apoptosis (P|0.01 in garlic treated; P|0.01 in tomato treated and P|0
Introduction: Aberrant crypt foci (ACF) are considered to be useful as surrogate biomarker for colorectal cancer (CRC), but the biological significance of ACF remains controversial. We attempted to investigate the relationship between the presence of ACF and human colorectal carcinogenesis using a relatively large sample size.. Methods: We performed high-magnification chromoscopic colonoscopy to identify ACF in 861 subjects undergoing a diagnostic endoscopy at the Yokohama City University Hospital. The present study compared the prevalence and number of ACF in three subject groups (normal subjects, adenoma cases, and CRC cases). The correlations between the demographic and behavioral characteristics of the subjects and the prevalence of ACF were also assessed.. Results: The prevalence of ACF was 64%, 88% and 95%, and the mean number of ACF was 3.6, 6.2 and 10.1, in normal subjects, adenoma cases, and CRC cases, respectively. Older age and men had increased prevalence and number of ACF. When ...
Full nutritional breakdown of the calories in Flaxseed Pizza Crust based on the calories and nutrition in each ingredient, including Natures Path Organic FlaxPlus Flaxseed meal, Olive Oil, Egg, fresh, whole, raw, Parmesan Cheese, grated, Baking Powder and the other ingredients in this recipe.
Full nutritional breakdown of the calories in flax bread based on the calories and nutrition in each ingredient, including Olive Oil, Egg, fresh, whole, raw, Bobs Red Mill Whole Ground Flaxseed Meal, 2 Tbsp., low carb baking mix, Baking Powder and the other ingredients in this recipe.
usr/bin/perl -w use Crypt::OpenPGP; use Crypt::OpenPGP::KeyRing; my($keyfile, $email) = @ARGV; my $ring = Crypt::OpenPGP::KeyRing-,new(Filename =, $keyfile); my $pgp = Crypt::OpenPGP-,new(PubRing =, $ring); my $kb = $ring-,find_keyblock_by_uid($email); my $alg = $kb-,preferred_sk_alg; my $crypt = $pgp-,encrypt( Data =, squeamish ossifrage, Recipients =, [ $email ], Armour =, 1, ($alg ? (Cipher =, $alg) : ()), # use default if no preference loc +ated ); print $crypt; exit 0; { package Crypt::OpenPGP::KeyBlock; sub preferred_sk_alg { my $self = shift; use Crypt::OpenPGP::Cipher; my $supported = Crypt::OpenPGP::Cipher-,supported; my @prefs; for my $sig (@{ $self-,get(Crypt::OpenPGP::Signature) }) { if ($sig-,is_primary) { unshift @prefs, @{ $sig-,preferred_sk_alg }; } else { push @prefs, @{ $sig-,preferred_sk_alg }; } } for (@prefs) { return $_ if $supported-,{$_}; } return; } } { package Crypt::OpenPGP::Signature; sub is_primary { my $self = shift; my $packet = $self-,find_subpacket(25); # ...
All sheet goods are not created equal, if your next project calls for these versatile materials, Bill Hylton has some suggestions on what to buy.
Dietary artepillin C suppresses the formation of aberrant crypt foci induced by azoxymethane in mouse colon.. Shimizu K, Das SK, Baba M, Matsuura Y, Kanazawa K.. Source. Department of Life Science, Graduate School of Science and Technology, Kobe University, Rokkodai, Nada-ku, Kobe 657-8501, Japan.. Abstract. Artepillin C, a prenylated phenylpropanoid found specifically in Brazilian propolis, has been shown to be a bioavailable antioxidant. In this study, artepillin C was tested for colon cancer-preventing activity using azoxymethane-challenged ddY mice. Oral doses of 80 and 160 mg/kg body weight of propolis or 10mg/kg of artepillin C (equi-amounts to 160 mg propolis) reduced significantly the frequency of colonic aberrant crypt foci (ACF) by 39.2, 43.7 and 43.4%, respectively. In liver of the mice, glutathione S-transferase and NADPH:quinone reductase activity increased with the doses of propolis or artepillin C, and an antioxidant-responsive element (ARE) was found to be activated for binding ...
Results A single bout of exercise increased the expression and secretion of SPARC in skeletal muscle in both mice and humans. In addition, in an azoxymethane-induced colon cancer mouse model, regular low-intensity exercise significantly reduced the formation of aberrant crypt foci in wild-type mice but not in SPARC-null mice. Furthermore, regular exercise enhanced apoptosis in colon mucosal cells and increased the cleaved forms of caspase-3 and caspase-8 in wild-type mice but not in SPARC-null mice. Culture experiments showed that SPARC secretion from myocytes was induced by cyclic stretch and inhibited proliferation with apoptotic effect of colon cancer cells.. ...
4975 Colon cancer is one of the major causes of cancer mortality in the United States of America. Early detection and prevention are the important alternative approaches to control colon cancer. Recently, substantial attention has been focused on identifying naturally occurring chemopreventive compounds capable of inhibiting early events involved in malignant transformation. Of late, laboratory studies have demonstrated that silibinin exhibits various biological activities and significantly inhibits tumorigenesis at various organ sites in several rodent models. The present study is an attempt to investigate the dose-dependent effect of dietary silibinin on azoxymethane (AOM)-induced aberrant crypt foci (ACF) formation in Fisher 344 male rats. In addition, we analyzed the effect of silibinin on cell proliferation and the induction of apoptosis by immunohistochemical staining during AOM-induced ACF formation. Five-week-old male F344 rats were randomly divided into 12 groups and fed AIN-76A control ...
Background: Aberrant crypt foci (ACF) are considered to be useful as surrogate biomarker for colorectal cancer (CRC), but the biological significance of ACF remains controversial. We attempted to investigate the relationship between the presence of ACF and human colorectal carcinogenesis using a relatively large sample size.. Methods: We carried out high-magnification chromoscopic colonoscopy to identify ACFs in 861 subjects undergoing a diagnostic endoscopy at the Yokohama City University Hospital. The present study compared the prevalence and number of ACFs in three subject groups (normal subjects, adenoma cases, and CRC cases). The correlations between the demographic and behavioral characteristics of the subjects and the prevalence of ACFs were also assessed.. Results: The prevalence of ACF was 64%, 88%, and 95%, and the mean number of ACF was 3.6, 6.2, and 10.1, in normal subjects, adenoma cases, and CRC cases, respectively. When differences in the prevalence and number of ACFs among age- ...
There is no consensus on what defines a reference for the world rice price. A review on rice as a differentiated commodity shed two important insights. First, it confirms that few studies have considered segmentation of rice in their price analysis. Second, Thai 5% brokens has often been considered the world reference price for rice but no empirical exercise has been carried out to validate this. This study analyzes the extent of market integration in the international rice market by generating empirical evidence on the cointegration of different export prices. We start our analysis with the assumption that rice is not a homogeneous good. In this context, we establish clusters by rice quality and determine which export markets best represent the world rice price within and across clusters. The study uses 19 monthly average export rice price quotations from January 2000 to July 2012 extracted from the FAO Global Information and Early Warning System food price database and from the Thai Rice Exporters
Double Bear brands rice flour is produced from high quality and selected species of Thai rice. It is sorted fromthe best rice growing regions and carefully stored and managed in accordance with international standards. Modern production process together with the knowhow that we have accumulated for over 35 years result in the best quality rice flour which meets a wide range of customers needs.. ...
Preheat oven at 375. Mix 2 T of flaxseed meal and water, set aside 3-5 minutes. Cream butter and sugar. Add eggs. Stir flaxseed mix into butter mix and add vanilla. Beat until well blended. Sift: dry ingredients, except oats and choc chips. Add butter mix to dry ing. Stir in the oats and then the choc chips. Drop on parchmant paper on baking sheet. Bake 8-12 minutes ...
Now I have traded oats for flaxseed meal and chia. Have you tried chia cereal? I reviewed chia seeds last fall (that post is HERE) and I really have enjoyed having them in my diet BUT recently found what I like even better -- Milled Chia -- the texture is much more *cereal*-like and not so seedy to me. ...
Index: php-7.2.12/ext/standard/config.m4 =================================================================== --- php-7.2.12.orig/ext/standard/config.m4 2018-11-06 11:48:33.000000000 +0100 +++ php-7.2.12/ext/standard/config.m4 2018-11-09 16:35:10.505160977 +0100 @@ -242,7 +242,7 @@ int main() { dnl dnl If one of them is missing, use our own implementation, portable code is then possible dnl -if test $ac_cv_crypt_blowfish = no ,, test $ac_cv_crypt_des = no ,, test $ac_cv_crypt_ext_des = no ,, test $ac_cv_crypt_md5 = no ,, test $ac_cv_crypt_sha512 = no ,, test $ac_cv_crypt_sha256 = no ,, test x$php_crypt_r = x0; then +if test $ac_cv_crypt_blowfish = no ,, test $ac_cv_crypt_des = no ,, test $ac_cv_crypt_md5 = no ,, test $ac_cv_crypt_sha512 = no ,, test $ac_cv_crypt_sha256 = no ,, test x$ac_cv_lib_crypt_crypt_r = x0; then dnl dnl Check for __alignof__ support in the ...
Culture Crypt presents the complete and definitive episode guide for Orphan Black, including a summary, synopsis, recap, and review of every episode from Season Two. Orphan Black premiered on BBC America in March of 2013, and stars Tatiana Maslany.
A60 The scientific potential of numerous unique animal models of carcinogenesis has not been fully realized due to our limited ability to monitor tumor growth in vivo. Establishment of a method for the accurate measurement of colon lesion size is needed to both characterize the dynamics of tumor growth and monitor responsiveness to therapeutic interventions. The goal of the present study was to develop an endoscopy-based protocol for the accurate estimation of tumor size in vivo from images obtained during colonoscopic examinations. This technology was established using a unique strain of Multiple Intestinal Neoplasia (Min) mice established at Fox Chase Cancer Center (Apc+/Min-FCCC), which spontaneously develop multiple colorectal tumors. Anesthetized Apc+/Min-FCCC mice received colonoscopic examinations, using a rigid endoscope. The endoscope (1.5 mm diameter) was inserted anally, and high-resolution images of 10 colon adenomas (from 8 animals) were captured using a CCD camera. Lesion size was ...
An object of this class encapsulates a passphrase hashed using the Blowfish-based Unix crypt() hash function, known as bcrypt. This is a subclass of Authen::Passphrase, and this document assumes that the reader is familiar with the documentation for that class.. The crypt() function in a modern Unix actually supports several different passphrase schemes. This class is concerned only with one particular scheme, a Blowfish-based algorithm designed by Niels Provos and David Mazieres for OpenBSD. To handle the whole range of passphrase schemes supported by the modern crypt(), see the from_crypt constructor and the as_crypt method in Authen::Passphrase.. The Blowfish-based crypt() scheme uses a variant of Blowfish called Eksblowfish, for expensive key schedule Blowfish. It has the cryptographic strength of Blowfish, and a very slow key setup phase to resist brute-force attacks. There is a cost parameter to the scheme: the length of key setup is proportional to 2^cost. There is a 128-bit salt. ...
TY - JOUR. T1 - Effect of diets fortified with tomatoes or onions with variable quercetin-glycoside content on azoxymethane-induced aberrant crypt foci in the colon of rats. AU - Femia, A.P.. AU - Caderni, G.. AU - Ianni, M.. AU - Salvadori, M.. AU - Schijlen, E.G.W.M.. AU - Collins, G.. AU - Bovy, A.G.. AU - Dolara, P.. PY - 2003. Y1 - 2003. N2 - Background: Onion and tomato are vegetables widely consumed by humans and epidemiological studies show an inverse association between vegetable consumption and colon cancer risk; however, the effect on colon cancer of diets containing high levels of vegetables like onion and tomato are not clear. Aims of the study: To investigate whether tomatoes and onions,with low or high quercetin-glycoside content, could reduce azoxymethane (AOM)-induced Aberrant Crypt Foci (ACF), preneoplastic lesions in the colon of rats. Methods: Male Fisher 344 rats were fed the following diets: a) high fat (HF) diet (control diet); b) HF diet containing 20 % (w/w) tomatoes ...
The modulating effect of the combined dietary feeding of β-carotene and perilla oil, which is rich in α- olinolenic acid, on the development of azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) was investigated in male F344 rats. Rats received oral administration of β-carotene (0, 50 or 200 mg/kg body weight/day) and fed a basal diet containing either 12% olive oil, 3% perilla oil plus 9% olive oil, or 12% perilla oil. A dose-dependent suppressive effect of perilla oil was found. The numbers of ACF were 42.0 and 18.4% of those of the 12% olive oil-fed controls in the rats fed 3% perilla oil plus 9% olive oil and 12% perilla oil, respectively. The development of ACF was also reduced significantly by the addition of dietary β-carotene in each of the oil-fed groups (P , 0.05, respectively). The suppression by the combination of β-carotene and perilla oil was synergistic, as the numbers of ACF were 12.9 and 8.9% of those of the 12% olive oil-fed controls in β-carotene-treated rats ...
Solid Gold SeaMeal Kelp-Based Overall Wellness Supplement Chew provides support for skin and coat, digestive and immune system health in dogs. Utilizing seaweed, earths original Superfood, Solid Gold SeaMeal combines red, green and brown seaweed species to deliver transformational health benefits for dogs and cats. Sourced from the coast of the United States, this carefully selected blend of seaweeds provides an excellent source of iodine and chlorophyll to provide important essential vitamins, minerals, amino acids and antioxidants for skin and coat health. Since coat and skin issues and digestive issues often go hand-in hand, digestive enzymes are added to support digestive health. Rich in Omega-3 Fatty Acids, flaxseed meal is included to provide additional support for skin and coat health. Feed this convenient and delicious chew once daily. Made in the USA. Grain and gluten free. No artificial colors, flavors or preservatives. Provides a natural support for three common pet health issues: skin and
Trail Mix Energy Balls With Old-fashioned Rolled Oats, Flaxseed Meal, Chia Seeds, Ground Cinnamon, Sea Salt, Creamy Peanut Butter, Honey, Vanilla Extract, Trail Mix
Mustard seeds (MS), which are consumed in considerable amounts by the Japanese people that, interestingly, have the longest life expectancy in the world, are known to contain a number of yet not fully defined but quite powerful anti-oxidants. A suspension of extracted MS was found to suppress oxidized-LDL-induced macrophage respiratory burst in vitro, to prevent growth, and to induce apoptotic death of SW480 cells (a human colon cancer cell line), while no such effects were found for normal 3T3 cells. A diet enriched with MS decreased plasma levels of the lipid peroxidation product malonaldehyde in mice exposed to the colon cancer-inducer azoxymethane (AOM). Such a diet also dose-dependently enhanced the activity of several anti-oxidant enzymes, such as superoxide dismutase (SOD), catalase, and GSH-peroxidase and, moreover, reduced AOM-mediated formation of colon adenomas by about 50%. Further studies are required to detail and explore the beneficial effects of MS and their rich content of ...
Cell cycle variations and DNA aneuploidy, were investigated in different phases of azoxymethane (AOM)-induced colon carcinogenesis in rats by flow cytometry. K-ras gene mutations (transitions Gright curved arrow A) were frequently detected in aberrant crypt foci (ACF) initial pre-neoplastic lesions. The fraction of cells in the G2M-phase of the cell cycle was higher in ACF compared to the normal mucosa of control rats. A similar modification of the cell cycle was found in adenomas and adenocarcinomas but, unexpectedly, also in morphologically normal mucosa from AOM-treated animals indicating that AOM treatment permanently modifies cell cycle control in rat colon mucosa. These alterations, however, were not associated with DNA aneuploidy as reported in human sporadic colorectal cancer, suggesting that tumour development in AOM-treated rats is less dependent on aneuploidy.. ...
TY - JOUR. T1 - Inhibition of carcinogenesis by seed and soybean meal extract in colon of mice. T2 - Apoptosis and dysplasia. AU - Amalia, Anindini Winda. AU - Kusmardi, Suid. AU - Elya, Berna. AU - Arsianti, Ade. PY - 2017/4/1. Y1 - 2017/4/1. N2 - Objective: Colon cancer is a major public health problem. Soybean has demonstrated chemopreventive and anticancer. Here, we have investigated the effect of a standardized seed and soybean meal extract (SE) with content of lunasin, here named grobogan extract (GE) and SE. They are botanical drug substance in experimental models of colon cancer in vivo. Methods: The effect of GE and SE was examined on the preneoplastic lesions (aberrant crypt foci), polyps and tumors induced by the carcinogenic agent azoxymethane (10 mg/kg) and dextran sodium sulfate 2% as well as in a xenograft model of colon cancer in mice. Results: GE and SE increased apoptosis (p=0.001). GE (150 mg/kg) has the highest impact level of apoptosis (p=0.009). GE and SE decreased ...
So I made these with some minor variations. First of all I doubled their size (made ten) and put them in a muffin tin to bake so I could use them as a sort of breakfast cookie. I ground almonds instead of using almond meal because I couldnt find any locally. I put in about 3 tbsp of ground flaxseed, I used raw sunflower seeds and chopped almonds as my nuts, organic ground coconut instead of flakes and I used baking raisins for the dried fruit. That being said I love the flavor and the texture but they are soft and falling apart just a little. Does everyone else find that they stay soft? Have you tried freezing them and then popping them in the microwave to defrost? I made so many and theyre really just for me to eat one every other day or so and I dont want them to go bad. The flaxseed meal will go bad I think if I dont freeze or at least refrigerate them. Im thinking I need a better binder so maybe soaking the ground flaxseed in a bit of juice before combining it with the ingredients might ...
So I made these with some minor variations. First of all I doubled their size (made ten) and put them in a muffin tin to bake so I could use them as a sort of breakfast cookie. I ground almonds instead of using almond meal because I couldnt find any locally. I put in about 3 tbsp of ground flaxseed, I used raw sunflower seeds and chopped almonds as my nuts, organic ground coconut instead of flakes and I used baking raisins for the dried fruit. That being said I love the flavor and the texture but they are soft and falling apart just a little. Does everyone else find that they stay soft? Have you tried freezing them and then popping them in the microwave to defrost? I made so many and theyre really just for me to eat one every other day or so and I dont want them to go bad. The flaxseed meal will go bad I think if I dont freeze or at least refrigerate them. Im thinking I need a better binder so maybe soaking the ground flaxseed in a bit of juice before combining it with the ingredients might ...
1. Preheat oven to 375ºF. Line or grease 12 muffin cups.. 2. In medium bowl, combine 3/4 cup all-purpose flour, whole-wheat flour, ½ cup brown sugar, flaxseed meal, baking powder, baking soda and salt; set aside.. 3. In large bowl, whisk buttermilk, ½ cup soybean oil, egg and vanilla. Pour into flour mixture; stir until just blended. Stir in blueberries until just blended. Spoon batter into prepared muffin cups, 3/4 full.. 4. In small bowl, mix remaining 2 T. all-purpose flour, 2 T. brown sugar and 1 T. soybean oil. Sprinkle over muffins.. 5. Bake 18-22 minutes until muffin edges and tops are golden.. ...
Melrose Omega Fibre is based on Australian Organic Flaxseed Meal, Licorice Root, Slippery Elm Bark Powder and Aloe Vera Juice Powder. Melrose Omega Fibre supports a gentle cleansing of the intestinal tract and may be taken on a daily basis without the wor
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"Mice overexpressing progastrin are predisposed for developing aberrant colonic crypt foci in response to AOM". American Journal ... "Activation of NF-κB is required for mediating proliferative and antiapoptotic effects of progastrin on proximal colonic crypts ...
... human gene Aberrant crypt foci, a preneoplastic lesion on the mucosa of colon or rectum Antecubital fossa, part of the ...
Pretlow TP, Pretlow TG (November 2005). "Mutant KRAS in aberrant crypt foci (ACF): initiation of colorectal cancer?". ...
... on colonic aberrant crypt foci formation in rats". Cancer Lett 107 (1): 137-142. PMID 8913278. doi:10.1016/0304-3835(96)04354-6 ...
... on colonic aberrant crypt foci formation in rats.". Cancer Lett. 107 (1): 137-142. PMID 8913278. doi:10.1016/0304-3835(96)04354 ...
... on colonic aberrant crypt foci formation in rats.". Cancer Lett 107 (1): 137-142. ...
Takayama T, Katsuki S, Takahashi Y, Ohi M, Nojiri S, Sakamaki S, Kato J, Kogawa K, Miyake H, Niitsu Y. Aberrant crypt foci of ... Aberrant crypt foci,ACF)的數量顯著增加[26]。異常腺窩病灶為大腸癌的
... on colonic aberrant crypt foci formation in rats. - Cancer Lett, 1996, kd 107, nr 1, lk 137-142. Resümee. Resümee. ...
Aberrant crypt foci. *Aphasiology. *Apoplexy. *Atypia. *Atypical small acinar proliferation. B. *Banff Classification ...
"Whole almonds and almond fractions reduce aberrant crypt foci in a rat model of colon carcinogenesis". Cancer Lett. 165 (1): 27 ...
... in drinking water enhance the growth of aberrant crypt foci in the mouse colon". 《Journal of Toxicology and Environmental ...
Aberrant DNA methylation patterns - hypermethylation and hypomethylation compared to normal tissue - have been associated with ... or on discrete neoplastic foci in vitro. Yet there is evidence that more than 80% of the somatic mutations found in mutator ... natural selection of a mutant or epigenetically altered cell among the stem cells at the base of one of the intestinal crypts ... Loss of that methylation can induce the aberrant expression of oncogenes, leading to cancer pathogenesis. Known mechanisms of ...
Many of the somatic evolutionary studies have traditionally been focused on clonal expansion, as recurrent types of changes can ... leading to the accumulation of aberrant cells. Most mammalian cells can replicate a limited number of times due to progressive ... predispose to clonal expansions that encompass large numbers of crypts in some conditions such as Barrett's esophagus. He also ... Studies of this issue have focused mainly at the gene mutation level, as copy number variation, LOH and specific chromosomal ...
The mutations can be inherited or acquired, and most probably occur in the intestinal crypt stem cell.[37][38][39] The most ... aberrant) methylation occurs as a normal consequence of normal aging and the risk of colorectal cancer increases as a person ... or on discrete neoplastic foci in vitro. Yet there is evidence that more than 80% of the somatic mutations found in mutator ...
Aberrant crypt foci (ACF) are one of the earliest histopathological manifestations of colon cancer. In this review, we ... Keywords: aberrant crypt foci; biomarker; colon cancer; chemoprevention aberrant crypt foci; biomarker; colon cancer; ... Aberrant Crypt Foci: The Case for Inclusion as a Biomarker for Colon Cancer. Michael J. Wargovich * , Vondina R. Brown. ... Wargovich, M.J.; Brown, V.R.; Morris, J. Aberrant Crypt Foci: The Case for Inclusion as a Biomarker for Colon Cancer. Cancers ...
CpG island methylation in aberrant crypt foci of the colorectum.. Chan AO1, Broaddus RR, Houlihan PS, Issa JP, Hamilton SR, ... Aberrant crypt foci (ACF) are postulated to be the earliest precursor lesion in colorectal carcinogenesis, and CpG island ...
All 20 subjects had aberrant crypt foci at base line.. RESULTS: We identified 3155 aberrant crypt foci, 161 of which were ... correlations between the number of aberrant crypt foci, the presence of dysplastic foci, the size of the foci, and the number ... Aberrant crypt foci of the colon as precursors of adenoma and cancer.. Takayama T1, Katsuki S, Takahashi Y, Ohi M, Nojiri S, ... Aberrant crypt foci of the colon are possible precursors of adenoma and cancer, but these lesions have been studied mainly in ...
The objective of the present study was to determine whether aberrant crypt foci (ACF) similar to those observed in the colons ... Twenty-six aberrant crypt foci displayed dysplasia as evident by histologic analysis. In these instances we feel the term ... Identification and quantification of aberrant crypt foci and microadenomas in the human colon Hum Pathol. 1991 Mar;22(3):287-94 ... The objective of the present study was to determine whether aberrant crypt foci (ACF) similar to those observed in the colons ...
... the chemopreventive effect of annatto (Bixa orellana L.) ... dietary administration of 1000 ppm annatto neither induce DNA damage in blood and colon cells nor aberrant crypt foci in rat ... on the formation of aberrant crypt foci (ACF) induced by dimethylhydrazine (DMH) in rat colon. Further, we investigate, the ... Conversely, annatto was successful in inhibiting the number of crypts/colon (animal), but not in the incidence of DMH-induced ...
Significance and Applicability of Aberrant Crypt Foci in Clinical Practice , IntechOpen, Published on: 2014-03-12. Authors: ... Aberrant crypts can be observed as single altered crypt or as a cluster of altered crypts that form a focus termed ACF [8; 14; ... Dysplastic aberrant crypts of human colorectal mucosa accompanying FAP (left). The focus is composed of 4 crypts. The level of ... Figure 3. Dysplastic aberrant crypts of human colorectal mucosa accompanying FAP (left). The focus is composed of 4 crypts. The ...
Crypts are 2 - 3x larger and more dilated than normal crypts, may have a foci of dysplasia * ACF can be subclassified as ... Cite this page: Jain S. Aberrant crypt foci (ACF) in colon. PathologyOutlines.com website. http://www.pathologyoutlines.com/ ... Endoscopy: aberrent cryptic foci are stained darker than normal crypts with Methylene blue using high magnification ...
... observed for foci with up to 4 crypts, but not for foci with 5 or more crypts. This may be explained by the fact that foci with ... Optimization of visibility and quantification of aberrant crypt foci in colonic mucosa in Wistar rats1 ... The emergence of aberrant crypt foci (ACF) is one of the most important outcome parameters in such models2. ... 5. Takayama T, Katsuki S, Takahashi Y, Ohi M, Nojiri S, Sakamaki S, Kato J, Kogawa, K, Miyake H, Niitsu Y. Aberrant crypt foci ...
Chemopreventive Potential of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside on the Formation of Aberrant Crypt Foci in ... β-D-glucoside on the Formation of Aberrant Crypt Foci in Azoxymethane-Induced Colorectal Cancer in Rats," BioMed Research ...
Changes in Total Aberrant Crypt Foci (ACF) Number [ Time Frame: Baseline to 30 days ]. Changes in total aberrant crypt foci ( ... values found in rectal aberrant crypt foci (ACF) tissue. *Post-treatment in Prostaglandin E2 (PGE2) Within Aberrant Crypt Foci ... Phase II A Trial of Curcumin Among Patients With Prevalent Subclinical Neoplastic Lesions (Aberrant Crypt Foci). This study has ... Aberrant Crypt Foci. Substance-Related Disorders. Chemically-Induced Disorders. Mental Disorders. Precancerous Conditions. ...
Ability of aberrant crypt foci characteristics to predict colonic tumor incidence in rats fed cholic acid MAGNUSON B. A. ... Regressive Effects of Various Chemopreventive Agents on Azoxymethane-induced Aberrant Crypt Foci in the Rat Colon * * MORISHITA ... Role of aberrant crypt foci in understanding the pathogenesis of colon cancer BIRD R. P. ... Piroxicam-induced regression of azoxymethane-induced aberrant crypt foci and prevention of colon cancer in rats PEREIRA M. A. ...
Background: Aberrant crypt foci (ACF) are considered to be useful as surrogate biomarker for colorectal cancer (CRC), but the ... Investigation of the Prevalence and Number of Aberrant Crypt Foci Associated with Human Colorectal Neoplasm. Eiji Sakai, ... Investigation of the Prevalence and Number of Aberrant Crypt Foci Associated with Human Colorectal Neoplasm ... Investigation of the Prevalence and Number of Aberrant Crypt Foci Associated with Human Colorectal Neoplasm ...
Somatic APC and K-ras Codon 12 Mutations in Aberrant Crypt Foci from Human Colons. Andrew J. Smith, Hartley S. Stern, Mark ... Aberrant crypt foci (ACF) are microscopic lesions which have been postulated to precede the development of adenomatous polyps, ... ras Codon 12 Mutations in Aberrant Crypt Foci from Human Colons. Andrew J. Smith, Hartley S. Stern, Mark Penner, Kazy Hay, ... ras Codon 12 Mutations in Aberrant Crypt Foci from Human Colons. Andrew J. Smith, Hartley S. Stern, Mark Penner, Kazy Hay, ...
The chemopreventive effects of Huangqin-tea against AOM-induced preneoplastic colonic aberrant crypt foci in rats and omics ... The chemopreventive effects of Huangqin-tea against AOM-induced preneoplastic colonic aberrant crypt foci in rats and omics ... we found that HQT inhibits azoxymethane-induced aberrant crypt foci (ACF) formation in a preneoplastic colonic ACF rat model. ...
Aberrant crypt foci AgNORs Pronyl-lysine Cell proliferation Chemoprevention This is a preview of subscription content, log in ... Bird RP (1995) Role of aberrant crypt foci in understanding the pathogenesis of colon cancer. Cancer Lett 93:55-71PubMed ... Aberrant crypt foci and AgNORs as putative biomarkers to evaluate the chemopreventive efficacy of pronyl-lysine in rat colon ... Agner AR, Bazo AP, Ribeiro LR, Salvadori DM (2005) DNA damage and aberrant crypt foci as putative biomarkers to evaluate the ...
Inhibition of azoxymethane-induced colonic aberrant crypt foci formation by silibinin in male Fisher 344 rats Velmurugan ... Inhibition of azoxymethane-induced colonic aberrant crypt foci formation by silibinin in male Fisher 344 rats ... Inhibition of azoxymethane-induced colonic aberrant crypt foci formation by silibinin in male Fisher 344 rats ... Inhibition of azoxymethane-induced colonic aberrant crypt foci formation by silibinin in male Fisher 344 rats ...
Phase IIA Trial of Curcumin Among Patients With Prevalent Subclinical Neoplastic Lesions (Aberrant Crypt Foci). Trial Phase:. ... Phase IIA Trial of Curcumin Among Patients With Prevalent Subclinical Neoplastic Lesions (Aberrant Crypt Foci) ...
GOMIDES, Antônio Frederico de Freitas et al. Defatted flaxseed meal prevents the appearance of aberrant crypt foci in the colon ... Palavras-chave : Defatted flaxseed; Aberrant crypt foci; P53 protein; Bifidobacterium spp. · resumo em Espanhol · texto em ... goal of this study was to investigate the preventive effects of defatted flaxseed meal on the appearance of aberrant crypt foci ... When we analyzed ACF with more than three crypts the group LIN and LINCA did not present ACF in the middle and distal segments ...
Then, the CEE was used to determine the effects on the progression of aberrant crypt foci (ACF), a preneoplastic lesion seen in ... Apoptosis Induction in Colon Cancer Cell Lines and Alteration of Aberrant Crypt Foci in Rat Colon by Purple Rice (Oryza sativa ... Rats fed with only the high dose of CEE had significantly decreased numbers of ACF per rat (45.56%) and crypt multiplicity (AC/ ... This finding could be concluded that purple rice extracts inhibited aberrant colonic epithelial cell progression via apoptosis ...
... of one crypt foci, 41% of two crypt foci, 18% of three crypt foci, and 21% of four or more crypt foci (Fig. 4A ). Rats fed β- ... Aberrant crypt multiplicity was determined as the number of crypts in each focus and categorized as containing up to four or ... A, normal crypt (10×), one crypt to four or more crypts (4×), and five crypts ACF (20×). B, effect of β-ionone on azoxymethane- ... Role of aberrant crypt foci in understanding the pathogenesis of colon cancer. Cancer Lett 1995;93:55-71. ...
1 to 9 crypts; medium, 10 to 19 crypts; and large, 20 crypts or more per focus; ref. 22; Fig. 1). All ACF were recorded ... Metformin Suppresses Colorectal Aberrant Crypt Foci in a Short-term Clinical Trial. Kunihiro Hosono, Hiroki Endo, Hirokazu ... Aberrant crypt foci of the colon as precursors of adenoma and cancer. N Engl J Med 1998;339:1277-84. ... Role of aberrant crypt foci in understanding the pathogenesis of colon cancer. Cancer Lett 1995;93:55-71. ...
"Sporadic aberrant crypt foci are not a surrogate endpoint for colorectal adenoma prevention" and "Aberrant crypt foci in the ... Sporadic aberrant crypt foci are not a surrogate endpoint for colorectal adenoma prevention. Cancer Prev Res 2008;1:4-8. ... Aberrant crypt foci as predictors of colorectal neoplasia on repeat colonoscopy. Cancer Causes Control 2012;23:355-61. ... Microsatellite instability in aberrant crypt foci from patients without concurrent colon cancer. Carcinogenesis 2007;28:769-76 ...
Herein, we investigated the mediation of AR in the formation of colonic preneoplastic aberrant crypt foci (ACF) using ... Herein, we investigated the mediation of AR in the formation of colonic preneoplastic aberrant crypt foci (ACF) using ...
Keywords: ACF, aberrant crypt foci; AOM, azoxymethane; DMH, 1,2-dimethylhydrazine; FAA, focal areas of atypism; H&E, ... Aberrant crypt foci (ACF), putative preneoplastic lesions, are early morphological changes induced by the colon carcinogen ... Aberrant crypt foci (ACF), putative preneoplastic lesions, are early morphological changes induced by the colon carcinogen ...
Aberrant crypt foci (ACF) are commonly considered the early pre-cancerous lesions that can progress to colorectal cancer (CRC ... Occurrence of colorectal aberrant crypt foci depending on age and dietary patterns of patients. *Marek Kowalczyk1. , ... Aberrant crypt foci (ACF) were first described by Bird in mice exposed to a mutagenic agent - azoxymethane in 1987 [1]. ... Assessment of rectal aberrant crypt foci by standard chromoscopy and its predictive value for colonic advanced neoplasm. Am J ...
Magnification endoscopy to detect dysplastic aberrant crypt foci in Mexican patients with high risk for colorectal cancer. * ... Magnification endoscopy to detect dysplastic aberrant crypt foci in Mexican patients with high risk for colorectal cancer. BMC ... The sequence of adenoma-cancer has been broadly documented, being accepted as the aberrant crypt-adenoma-cancer concept. These ...
Our results suggest that Apc mutations lead to an increase in Prom1 expressing cells in the intestinal crypt stem cell ... Our results suggest that Apc mutations lead to an increase in Prom1 expressing cells in the intestinal crypt stem cell ... Aberrant Crypt Foci. Aberrant crypt foci (ACF) were induced using AOM and DSS. Six-week-old female Wt and Prom1-/- mice (N = 5 ... Increased colonic inflammatory injury and formation of aberrant crypt foci in Nrf2-deficient mice upon dextran sulfate ...
Aberrant Crypt Foci as a Biomarker for Chemoprevention. *Sinicrope, Frank A (PI) ... A promising candidate SEB is the aberrant crypt foci (ACF). ACF are putative precursors of adenomas and carcinomas based upon ...
A Phase IIa Randomized, Double-Blind Trial of Erlotinib in Inhibiting EGF Receptor Signaling in Aberrant Crypt Foci of the ... A Phase IIa Randomized, Double-Blind Trial of Erlotinib in Inhibiting EGF Receptor Signaling in Aberrant Crypt Foci of the ... Focus Biomarker; Therapeutic Use Most Recent Events * 07 Feb 2013 Actual initiation date changed from Sep 2008 to Jul 2008 as ...
A Risk Assessment Using Aberrant Crypt Foci. Riccardo Nascimbeni, Francesco Donato, Mauro Ghirardi, Pierpaolo Mariani, Vincenzo ... of foci/cm2), and multiplicity (no. of crypts/focus) of ACF were recorded in each colonic specimen. Because foci ,10 crypts ... Bird R. P. Role of aberrant crypt foci in understanding the pathogenesis of colon cancer. Cancer Lett., 93: 55-71, 1995. ... Kostantakos A. K., Siu I., Pretlow T. G., Stellato T. A., Pretlow T. P. Human aberrant crypt foci with carcinoma in situ from a ...
  • Aberrant crypt foci of the colon are possible precursors of adenoma and cancer, but these lesions have been studied mainly in surgical specimens from patients who already had colon cancer. (nih.gov)
  • Aberrant crypt foci (ACF) are microscopic lesions which have been postulated to precede the development of adenomatous polyps, the precursors to colorectal cancer. (aacrjournals.org)
  • ACF are tiny lesions that develop in the earliest stage of colorectal carcinogenesis and consist of large, thick crypts identified by dense methylene blue staining ( 18 - 21 ). (aacrjournals.org)
  • Aberrant crypt foci (ACF), putative preneoplastic lesions, are early morphological changes induced by the colon carcinogen azoxymethane (AOM). (oup.com)
  • Aberrant crypt foci (ACF) are commonly considered the early pre-cancerous lesions that can progress to colorectal cancer (CRC). (biomedcentral.com)
  • Aberrant crypt foci (ACF) are microscopic lesions of the colonic mucosa suspected of being preneoplastic, and their investigation has been advocated to evaluate the cause-effect relationship between putative risk factors and CRC. (aacrjournals.org)
  • Aims of the study: To investigate whether tomatoes and onions,with low or high quercetin-glycoside content, could reduce azoxymethane (AOM)-induced Aberrant Crypt Foci (ACF), preneoplastic lesions in the colon of rats. (wur.nl)
  • aberrant crypt foci (ACF) are colon preneoplastic lesions that can be used as a tool to study preventive processes for colorectal cancer (CRC). (isciii.es)
  • We therefore investigated whether a 1-year administration of different l -carnitine concentrations (0, 1, 2 and 5 g/l) via drinking water leads to an increased incidence of preneoplastic lesions (so-called aberrant crypt foci ) in the colon of Fischer 344 rats as well as to the appearance of atherosclerotic lesions in the aorta of these animals. (springer.com)
  • In this paper, we investigated the involvement of endocannabinoids on the formation of aberrant crypt foci (ACF, earliest preneoplastic lesions) in the colon mouse in vivo. (420magazine.com)
  • Development of colon cancer is a multistep process involving a series of pathological alterations ranging from discrete microscopic mucosal lesions, like aberrant crypt foci (ACF), to malignant tumors [2]. (420magazine.com)
  • ACF are early focal lesions of the colonic mucosa composed of one to several enlarged crypts, which are specifically induced by colon carcinogens [3]. (420magazine.com)
  • Hyperplastic aberrant crypts of human (left) colorectal mucosa accompanying resected sporadic colorectal adenoma. (intechopen.com)
  • On the right there are hyperplastic aberrant crypts of rat colorectal mucosa, induced by carcinogen. (intechopen.com)
  • The level of focus is higher then of the surrounding mucosa. (intechopen.com)
  • Dysplastic aberrant crypts of human colorectal mucosa accompanying FAP (left). (intechopen.com)
  • Test immersion of microscopy samples in water as an aid to visualizing and quantifying aberrant crypt foci (ACF) in rat colon mucosa. (scielo.br)
  • Immersing microscopy samples in water aids the visualization and quantification of aberrant crypt foci in rat colon mucosa fixed in formaldehyde. (scielo.br)
  • ACF in colonic mucosa stained with toluidine blue is usually darker and larger than normal crypt foci and tend to occur in isolation, in pairs or in small clusters. (scielo.br)
  • One hundred and fifty-four ACF comprised of between 1 and 56 crypts were harvested from the grossly normal mucosa of colorectal resection specimens of 28 patients with varying pathological diagnoses. (aacrjournals.org)
  • Aberrant crypt foci (ACF) are morphologically identifiable mucosal abnormalities, a subset of which may be precancerous and contribute to a "field defect" within the mucosa ( 5 ). (aacrjournals.org)
  • The intestinal tract is lined by mucosa, which is primarily composed of villi or the intercrypt table and epithelial-lined crypts at the mucosal base ( 1 - 4 ). (frontiersin.org)
  • Clusters of colonic crypts that appear different from the surrounding mucosa when visualized after staining. (bioportfolio.com)
  • To determine the effect of the study drugs (aspirin [acetylsalicylic acid] + DFMO) and placebo with respect to proliferation (Ki67 labeling index), apoptosis (caspase-3 expression assay), and drug effect markers (COX-1, -2, polyamines, PGE2) from adenomas, aberrant crypt focus (ACF) and normal-appearing mucosa using pre- and 12-month post-intervention tissue biopsy samples. (clinicaltrials.gov)
  • I. To explore the effects of the study agents on a focused panel of tissue biomarkers in pre- and post-intervention biopsy samples from recurrent adenomas, rectal ACF, and adjacent normal-appearing mucosa among subjects enrolled in the phase II clinical trial. (clinicaltrials.gov)
  • Our aim was to study the chemopreventive efficacy of pronyl-lysine, a key antioxidant present in bread crust by evaluating, the total number of aberrant crypt foci (ACF), their distributions, dysplastic ACF, colonic tumor incidence and the expression of cell proliferation biomarker such as the argyrophilic nucleolar organizing region-associated proteins (AgNORs) in 1,2-dimethylhydrazine (DMH) induced colon cancer in rats. (springer.com)
  • Deeptha K, Kamaleeswari M, Sengottuvelan M, Nalini N (2006) Dose dependent inhibitory effect of dietary caraway on 1,2-dimethylhydrazine induced colonic aberrant crypt foci and bacterial enzyme activity in rats. (springer.com)
  • The present study is an attempt to investigate the dose-dependent effect of dietary silibinin on azoxymethane (AOM)-induced aberrant crypt foci (ACF) formation in Fisher 344 male rats. (aacrjournals.org)
  • Then, the CEE was used to determine the effects on the progression of aberrant crypt foci (ACF), a preneoplastic lesion seen in colon carcinogenesis in rats. (biomedsearch.com)
  • Rats fed with only the high dose of CEE had significantly decreased numbers of ACF per rat (45.56%) and crypt multiplicity (AC/focus) (16.67%) compared to rats that received DMH alone. (biomedsearch.com)
  • Colons of 10 rats from each dietary group were analyzed for aberrant crypt foci (ACF), which are preneoplastic markers. (uoi.gr)
  • Colonic mucosal proliferation, aberrant crypt foci (ACF) induction, and fecal bile acids, parameters connected to the risk of colon cancer development, were studied in female F344 rats treated with starch or sucrose boluses or with a sucrose diet. (naver.com)
  • In additional experiments, colon cell proliferation (MF/crypt) was higher in rats given boluses of sucrose three times per week for 40 days after AOM (20 mg/kg) [5.9 ± 0.7 (SE)] than in rats given starch boluses (2.96 ± 0.4) or fed sucrose continuously (3.6 ± 0.5). (naver.com)
  • This study investigatedthe effects of Amorphophallus campanulatus tuber methanolic extract (ACME) on aberrant crypt foci (ACF)formation, colonic cell proliferation, lipid peroxidative damage and the antioxidant status in a long term preclinicalmodel of 1, 2-dimethylhydrazine (DMH) induced colon carcinogenesis in rats. (waocp.org)
  • In this study, the chemopreventive potential of Cranberry was analyzed in reducing the Aberrant Crypt Foci (ACF) induced by Azoxymethane (AOM) in Fisher 344 male rats. (cranberryinstitute.org)
  • Sengottuvelan M, Viswanathan P, Nalini N (2006) Chemopreventive effect of trans-resveratrol-a phytoalexin against colonic aberrant crypt foci and cell proliferation in 1,2-dimethylhydrazine induced colon carcinogenesis. (springer.com)
  • Cell proliferation was higher in animals treated with a single sucrose bolus than in those given a starch bolus (15 g/kg body wt), with 4.3 ± 0.64 and 2.17 ± 0.57 (SE) mitotic figures (MF) per crypt in the sucrose and starch bolus groups, respectively (p (naver.com)
  • Blume Tuber Against Aberrant Crypt Foci and Cell Proliferation in 1, 2-Dimethylhydrazine Induced Colon Carcinogenesis', Asian Pacific Journal of Cancer Prevention , 14(9), pp. 5331-5339. (waocp.org)
  • The excised colons were evaluated for aberrant crypt foci (ACF) formation, cell proliferation (PCNA assay), apoptosis (TUNEL assay), and iNOS and COX-2 expression. (tamu.edu)
  • Generalized disruption of Wnt signaling in the intestine resulted in widespread loss of crypts with the absence of cell proliferation in the small intestine, indicating an essential role for this pathway in the maintenance of intestinal stem cells and their dividing descendents. (pnas.org)
  • This reduction in aberrant crypt foci (ACF) was accompanied by suppression of cell proliferation (mean Brdu LI in carcinogen control=13.91+/-3.31, and 0.5% cardamom=2.723+/-0.830) and induction of apoptosis (mean AI in carcinogen control=1.547+/-0.42 and 0.5% cardamom=6.61+/-0.55). (sigmaaldrich.com)
  • 0.001) correlations between the number of aberrant crypt foci, the presence of dysplastic foci, the size of the foci, and the number of adenomas. (nih.gov)
  • Our results suggest that Apc mutations lead to an increase in Prom1 expressing cells in the intestinal crypt stem cell compartment and in early intestinal adenomas. (frontiersin.org)
  • Aberrant crypt foci (ACF) are putative precursors of colorectal adenomas and have been postulated as a potential biomarker for colorectal cancer. (wustl.edu)
  • We counted aberrant crypt foci (ACF), mucin depleted foci (MDF) and adenomas in the colon. (nih.gov)
  • 15 However, while microsatellite instability (MSI) may be established at an early stage in adenoma formation, including microscopic aberrant crypt foci, 2 16 17 mutated genes have not been detected at high frequency in adenomas showing MSI. (bmj.com)
  • The adenoma to carcinoma sequence is characterized by recognizable histologic changes that start with dysplastic aberrant crypt foci and benign tubular adenomas. (aacrjournals.org)
  • The aberrant methylation of certain tumor suppressor genes, such as CDKN2A , has been reported in colonic adenomas and aberrant crypt foci (7-9) . (aacrjournals.org)
  • The Aspirin/Folate Polyp Prevention Study reported that folic acid supplementation at 1 mg/day for 6-10 years significantly increased the risk of recurrence of advanced and multiple colorectal adenomas [15] and of prostate cancer [16] in predisposed subjects with prior colorectal adenomas who likely harboured preneoplastic foci. (plos.org)
  • Aberrant crypt foci (ACF) are postulated to be the earliest precursor lesion in colorectal carcinogenesis, and CpG island methylation has been described as an important molecular pathway. (nih.gov)
  • DNA damage and aberrant crypt foci as putative biomarkers to evaluate the chemopreventive effect of annatto (Bixa orellana L.) in rat colon carcinogenesis. (greenmedinfo.com)
  • We tested the in vivo chemopreventive efficacy in rat colon carcinogenesis model using aberrant crypt foci (ACF) as endpoint marker. (aacrjournals.org)
  • We also examine the chemopreventive efficacy of β-ionone on azoxymethane-induced rat colon carcinogenesis model using colonic aberrant crypt foci (ACF) as endpoint biomarker. (aacrjournals.org)
  • Inoculation with La reduced the severity of colonic carcinogenesis caused by CT-26 cells, such as level of colonic involvement and structural abnormality of epithelial/crypt damage. (cambridge.org)
  • Moreover, analysis of preneoplastic aberrant crypt foci from high-risk patients suggests that acquisition of the high-COX-2/low-cPLA 2 phenotype may arise at an early stage of colon carcinogenesis. (aacrjournals.org)
  • Aberrant crypt foci of the colon as precursors of adenoma and cancer. (nih.gov)
  • Using magnifying endoscopy, we studied the prevalence, number, size, and dysplastic features of aberrant crypt foci and their distribution according to age in 171 normal subjects, 131 patients with adenoma, and 48 patients with colorectal cancer. (nih.gov)
  • We also prospectively examined the prevalence of aberrant crypt foci in 11 subjects (4 normal subjects, 6 with adenoma, and 1 with cancer) before and after the administration of 100 mg of sulindac three times a day for 8 to 12 months and compared the results with those in 9 untreated subjects (4 normal subjects and 5 with adenoma). (nih.gov)
  • Aberrant crypt foci, particularly those that are large and have dysplastic features, may be precursors of adenoma and cancer. (nih.gov)
  • The sequence of adenoma-cancer has been broadly documented, being accepted as the aberrant crypt-adenoma-cancer concept. (biomedcentral.com)
  • Analysis of K-ras, APC, and β-catenin in aberrant crypt foci in sporadic adenoma, cancer, and familial adenomatous polyposis (2001) Takayama Tetsuji et al. (naver.com)
  • An aberrant crypt focus obtains a KRAS mutation and progresses to an early adenoma. (brainscape.com)
  • Determine the aberrant crypt foci (ACF) and adenoma burden in the entire colorectum of these patients. (clinicaltrials.gov)
  • Aberrant crypt foci (ACF) are considered to be useful as surrogate biomarker for colorectal cancer (CRC), but the biological significance of ACF remains controversial. (aacrjournals.org)
  • Results indicate that feeding of lactulose and B. longum singly and in combination reduces the number of ACF (P = 0.0001) and the total number of aberrant crypts significantly (P = 0.0005). (uoi.gr)
  • The mucosal epithelial cells arise from intestinal stem cells located in the crypt ( 5 ). (frontiersin.org)
  • When looking for aberrant crypt foci with microscopy, methylene blue is used as a staining agent. (wikipedia.org)
  • There was a close correlation between the number of crypts per ACF in each specimen as scored by methylene-blue and histologic examination. (nih.gov)
  • Twenty-six aberrant crypt foci displayed dysplasia as evident by histologic analysis. (nih.gov)
  • Notably, N-arachidonoylserotonin completely prevented the formation of ACF with four or more crypts, which have been show to be best correlated with final tumor incidence. (420magazine.com)
  • In the present study, we evaluate the activity of annatto (Bixa orellana L.), a natural food colorant rich in carotenoid, on the formation of aberrant crypt foci (ACF) induced by dimethylhydrazine (DMH) in rat colon. (greenmedinfo.com)
  • Here, we found that HQT inhibits azoxymethane-induced aberrant crypt foci (ACF) formation in a preneoplastic colonic ACF rat model. (rsc.org)
  • 0.001) reduces the total number and crypt multiplicity of AOM-induced ACF formation in a dose-dependent manner. (aacrjournals.org)
  • Furthermore, we showed that metformin suppressed the azoxymethane-induced formation of colorectal aberrant crypt foci (ACF) by activating AMPK ( 17 ). (aacrjournals.org)
  • Dietary artepillin C suppresses the formation of aberrant crypt foci induced by azoxymethane in mouse colon. (propolisscience.org)
  • 7 crypts) decreased with increasing dose of FaOH and FaDOH and that this inhibitory effect on early neoplastic formation of ACF was dose-dependent, which was also the case for the total number of macroscopic neoplasms. (aau.dk)
  • Resveratrol glucoside from transgenic alfalfa has been used for the prevention of aberrant crypt foci in mice. (wikipedia.org)
  • The goal of this study was to investigate the preventive effects of defatted flaxseed meal on the appearance of aberrant crypt foci (ACF) in mice treated with 1,2-dimethylhydrazine (DMH). (isciii.es)
  • Aberrant crypt foci (ACF) were first described by Bird in mice exposed to a mutagenic agent - azoxymethane in 1987 [ 1 ]. (biomedcentral.com)
  • Journal Article] Lack of enhancing effects of degraded λ-carrageenan on the development of β-catenin-accumulated crypts in male DBA/2J mice initiated with azoxymethane. (nii.ac.jp)
  • CpG island methylation in aberrant crypt foci of the colorectum. (nih.gov)
  • CpG island DNA methylation is an epigenetic mechanism that represses gene transcription in a variety of normal cellular processes including imprinting and X-chromosome inactivation, but it becomes excessive and aberrant in many neoplasms. (aacrjournals.org)
  • The aberrant DNA methylation affects CpG-rich regions, called "CpG islands," in the 5′ region of genes and results in transcriptional silencing through complex effects on transcription factor binding and associated changes in chromatin structure. (aacrjournals.org)
  • Field cancerization in the colon: a role for aberrant DNA methylation? (semanticscholar.org)
  • The epithelial cells of the hyperplastic crypts are higher and composed of one layer. (intechopen.com)
  • The epithelial cells covering the aberrant crypts are classified as mild dysplasia. (intechopen.com)
  • Metformin has also been shown to suppress proliferation of colon epithelial cells and aberrant crypt foci (ACF). (healthcanal.com)
  • Aberrant crypt foci (ACF) are clusters of abnormal tube-like glands in the lining of the colon and rectum. (wikipedia.org)
  • Under such conditions, dietary administration of 1000 ppm annatto neither induce DNA damage in blood and colon cells nor aberrant crypt foci in rat distal colon. (greenmedinfo.com)
  • Research on diet in breast cancer survival has been focused on single nutrients or foods, particularly dietary fat, fruits, vegetables, fiber, and alcohol. (google.com)
  • The objective of the present study was to determine whether aberrant crypt foci (ACF) similar to those observed in the colons of experimental animals exposed to colon carcinogens could be identified and quantified in the human colon. (nih.gov)
  • Frequent and characteristic K- ras activation and absence of p53 protein accumulation in aberrant crypt foci of the colon (1994) Yamashita Naoyuki et al. (naver.com)
  • Carcinoembryonic antigen in human colonic aberrant crypt foci (1994) Pretlow Theresa P et al. (naver.com)
  • This finding could be concluded that purple rice extracts inhibited aberrant colonic epithelial cell progression via apoptosis induction. (biomedsearch.com)
  • compared with control (C-). In relation to the weight of the organs, the groups LIN and LINCA showed higher values for the liver and kidney compared with control (C-). Regarding ACF, the group LINCA presented fewer ACF in the middle and distal colon compared with control (C-). When we analyzed ACF with more than three crypts the group LIN and LINCA did not present ACF in the middle and distal segments. (isciii.es)
  • Aberrant crypts are characterized by irregularly shaped glands (crypts), with enlarged luminal opening and cell hyperplasia. (scielo.br)
  • 1991. Control of 1,2-dimethylhydrazine-induced crypt hyperplasia by naturalkiller cells and its relevance to carcinogenics. (cdc.gov)
  • The focus is composed of 3 crypts with slight mucin depletion. (intechopen.com)
  • The focus is composed of 3 crypts that show severe mucin depletion. (intechopen.com)
  • These results show that aberrant methylated genes can be detected frequently in sporadic colon polyps and that they can be detected in fecal DNA. (aacrjournals.org)
  • Aberrant crypt foci form before colorectal polyps and are one of the earliest changes seen in the colon that may lead to cancer. (wikipedia.org)
  • Recently, substantial attention has been focused on identifying naturally occurring chemopreventive compounds capable of inhibiting early events involved in malignant transformation. (aacrjournals.org)
  • We measured intestinal weight, cross sectional area, villus height and crypt depth. (nih.gov)
  • Thus, expression of Ngn3 and NeuroD represent distinct stages in the differentiation of enteroendocrine cells from intestinal secretory progenitor cells in intestinal crypts. (pnas.org)
  • Oral doses of 80 and 160 mg/kg body weight of propolis or 10mg/kg of artepillin C (equi-amounts to 160 mg propolis) reduced significantly the frequency of colonic aberrant crypt foci (ACF) by 39.2, 43.7 and 43.4%, respectively. (propolisscience.org)
  • Our research focuses on the identification of natural substances, such as phytoestrogens, that significantly contribute in cancer prevention and/or treatment. (ucy.ac.cy)
  • Conversely, annatto was successful in inhibiting the number of crypts/colon (animal), but not in the incidence of DMH-induced ACF, mainly when administered after DMH. (greenmedinfo.com)