A replication-defective strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) capable of transforming lymphoid cells and producing a rapidly progressing lymphoid leukemia after superinfection with FRIEND MURINE LEUKEMIA VIRUS; MOLONEY MURINE LEUKEMIA VIRUS; or RAUSCHER VIRUS.
Species of GAMMARETROVIRUS, containing many well-defined strains, producing leukemia in mice. Disease is commonly induced by injecting filtrates of propagable tumors into newborn mice.
Transforming proteins encoded by the abl oncogenes. Oncogenic transformation of c-abl to v-abl occurs by insertional activation that results in deletions of specific N-terminal amino acids.
A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) arising during the propagation of S37 mouse sarcoma, and causing lymphoid leukemia in mice. It also infects rats and newborn hamsters. It is apparently transmitted to embryos in utero and to newborns through mother's milk.
An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.
Leukemia induced experimentally in animals by exposure to leukemogenic agents, such as VIRUSES; RADIATION; or by TRANSPLANTATION of leukemic tissues.
A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) isolated from spontaneous leukemia in AKR strain mice.
A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) producing leukemia of the reticulum-cell type with massive infiltration of liver, spleen, and bone marrow. It infects DBA/2 and Swiss mice.
Retrovirus-associated DNA sequences (abl) originally isolated from the Abelson murine leukemia virus (Ab-MuLV). The proto-oncogene abl (c-abl) codes for a protein that is a member of the tyrosine kinase family. The human c-abl gene is located at 9q34.1 on the long arm of chromosome 9. It is activated by translocation to bcr on chromosome 22 in chronic myelogenous leukemia.
A neoplasm originating from thymic tissue, usually benign, and frequently encapsulated. Although it is occasionally invasive, metastases are extremely rare. It consists of any type of thymic epithelial cell as well as lymphocytes that are usually abundant. Malignant lymphomas that involve the thymus, e.g., lymphosarcoma, Hodgkin's disease (previously termed granulomatous thymoma), should not be regarded as thymoma. (From Stedman, 25th ed)
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
Viruses which enable defective viruses to replicate or to form a protein coat by complementing the missing gene function of the defective (satellite) virus. Helper and satellite may be of the same or different genus.
Established cell cultures that have the potential to propagate indefinitely.
Genes whose gain-of-function alterations lead to NEOPLASTIC CELL TRANSFORMATION. They include, for example, genes for activators or stimulators of CELL PROLIFERATION such as growth factors, growth factor receptors, protein kinases, signal transducers, nuclear phosphoproteins, and transcription factors. A prefix of "v-" before oncogene symbols indicates oncogenes captured and transmitted by RETROVIRUSES; the prefix "c-" before the gene symbol of an oncogene indicates it is the cellular homolog (PROTO-ONCOGENES) of a v-oncogene.
The functional hereditary units of VIRUSES.
A species of GAMMARETROVIRUS causing leukemia, lymphosarcoma, immune deficiency, or other degenerative diseases in cats. Several cellular oncogenes confer on FeLV the ability to induce sarcomas (see also SARCOMA VIRUSES, FELINE).
Proteins found in any species of virus.
Species of GAMMARETROVIRUS isolated from fibrosarcoma in cats. The viruses are actually recombinant feline leukemia viruses (FeLV) where part of the genome has been replaced by cellular oncogenes. It is unique to individuals and not transmitted naturally to other cats. FeSVs are replication defective and require FeLV to reproduce.
A strain of MURINE LEUKEMIA VIRUS associated with mouse tumors similar to those caused by the FRIEND MURINE LEUKEMIA VIRUS. It is a replication-competent murine leukemia virus. It can act as a helper virus when complexing with a defective transforming component, RAUSCHER SPLEEN FOCUS-FORMING VIRUS.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
Virus diseases caused by the RETROVIRIDAE.
The type species of DELTARETROVIRUS that causes a form of bovine lymphosarcoma (ENZOOTIC BOVINE LEUKOSIS) or persistent lymphocytosis.
Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Deoxyribonucleic acid that makes up the genetic material of viruses.
Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors.
A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)
Viruses which lack a complete genome so that they cannot completely replicate or cannot form a protein coat. Some are host-dependent defectives, meaning they can replicate only in cell systems which provide the particular genetic function which they lack. Others, called SATELLITE VIRUSES, are able to replicate only when their genetic defect is complemented by a helper virus.
Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.
A general term for various neoplastic diseases of the lymphoid tissue.
Strains of MURINE LEUKEMIA VIRUS discovered in 1976 by Hartley, Wolford, Old, and Rowe and so named because the viruses originally isolated had the capacity to transform cell foci in mink cell cultures. MCF viruses are generated by recombination with ecotropic murine leukemia viruses including AKR, Friend, Moloney, and Rauscher, causing ERYTHROLEUKEMIA and severe anemia in mice.
An enzyme that synthesizes DNA on an RNA template. It is encoded by the pol gene of retroviruses and by certain retrovirus-like elements. EC 2.7.7.49.
Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Duplex DNA sequences in eukaryotic chromosomes, corresponding to the genome of a virus, that are transmitted from one cell generation to the next without causing lysis of the host. Proviruses are often associated with neoplastic cell transformation and are key features of retrovirus biology.
Proteins coded by the retroviral gag gene. The products are usually synthesized as protein precursors or POLYPROTEINS, which are then cleaved by viral proteases to yield the final products. Many of the final products are associated with the nucleoprotein core of the virion. gag is short for group-specific antigen.
Infections produced by oncogenic viruses. The infections caused by DNA viruses are less numerous but more diverse than those caused by the RNA oncogenic viruses.
A genus of RETROVIRIDAE comprising endogenous sequences in mammals, related RETICULOENDOTHELIOSIS VIRUSES, AVIAN, and a reptilian virus. Many species contain oncogenes and cause leukemias and sarcomas.
Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.
Insertion of viral DNA into host-cell DNA. This includes integration of phage DNA into bacterial DNA; (LYSOGENY); to form a PROPHAGE or integration of retroviral DNA into cellular DNA to form a PROVIRUS.
A species of GAMMARETROVIRUS causing leukemia in the gibbon ape. Natural transmission is by contact.
Ribonucleic acid that makes up the genetic material of viruses.
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Layers of protein which surround the capsid in animal viruses with tubular nucleocapsids. The envelope consists of an inner layer of lipids and virus specified proteins also called membrane or matrix proteins. The outer layer consists of one or more types of morphological subunits called peplomers which project from the viral envelope; this layer always consists of glycoproteins.
Carnivores of genus Mustela of the family MUSTELIDAE. The European mink, which has white upper and lower lips, was widely trapped for commercial purposes and is classified as endangered. The American mink, lacking a white upper lip, is farmed commercially.
The class of heavy chains found in IMMUNOGLOBULIN M. They have a molecular weight of approximately 72 kDa and they contain about 57 amino acid residues arranged in five domains and have more oligosaccharide branches and a higher carbohydrate content than the heavy chains of IMMUNOGLOBULIN G.
A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Conditions in which the abnormalities in the peripheral blood or bone marrow represent the early manifestations of acute leukemia, but in which the changes are not of sufficient magnitude or specificity to permit a diagnosis of acute leukemia by the usual clinical criteria.
A strain of PRIMATE T-LYMPHOTROPIC VIRUS 1 isolated from mature T4 cells in patients with T-lymphoproliferation malignancies. It causes adult T-cell leukemia (LEUKEMIA-LYMPHOMA, T-CELL, ACUTE, HTLV-I-ASSOCIATED), T-cell lymphoma (LYMPHOMA, T-CELL), and is involved in mycosis fungoides, SEZARY SYNDROME and tropical spastic paraparesis (PARAPARESIS, TROPICAL SPASTIC).
Ordered rearrangement of B-lymphocyte variable gene regions of the IMMUNOGLOBULIN HEAVY CHAINS, thereby contributing to antibody diversity. It occurs during the first stage of differentiation of the IMMATURE B-LYMPHOCYTES.
Any discrete, presumably solitary, mass of neoplastic PLASMA CELLS either in BONE MARROW or various extramedullary sites.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Retroviral proteins, often glycosylated, coded by the envelope (env) gene. They are usually synthesized as protein precursors (POLYPROTEINS) and later cleaved into the final viral envelope glycoproteins by a viral protease.
Retroviral proteins that have the ability to transform cells. They can induce sarcomas, leukemias, lymphomas, and mammary carcinomas. Not all retroviral proteins are oncogenic.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
A group of replication-defective viruses, in the genus GAMMARETROVIRUS, which are capable of transforming cells, but which replicate and produce tumors only in the presence of Murine leukemia viruses (LEUKEMIA VIRUS, MURINE).
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.

p53 mediates apoptotic crisis in primary Abelson virus-transformed pre-B cells. (1/205)

Transformation of pre-B cells by Abelson murine leukemia virus (Ab-MLV) involves a balance between positive, growth-stimulatory signals from the v-Abl oncoprotein and negative regulatory cues from cellular genes. This phenomenon is reflected by the clonal selection that occurs during Ab-MLV-mediated transformation in vivo and in vitro. About 50% of all Ab-MLV-transformed pre-B cells express mutant forms of p53 as they emerge from this process, suggesting that this protein may play an important role in the transformation process. Consistent with this idea, expression of p19(Arf), a protein whose function depends on the presence of a functional p53, is required for the apoptotic crisis that characterizes primary Ab-MLV transformants. To test the role of p53 in pre-B-cell transformation directly, we examined the response of Trp53(-/-) mice to Ab-MLV. The absence of p53 shortens the latency of Abelson disease induction but does not affect the frequency of cells susceptible to Ab-MLV-induced transformation. However, primary transformants derived from the null animals bypass the apoptotic crisis that characterizes the transition from primary transformant to fully malignant cell line. These effects do not require p21(Cip-1), a major downstream target of p53; however, consistent with a role of p19(Arf), transformants expressing mutant p53 and abundant p19 retain wild-type p19 sequences.  (+info)

Functional characterization of B lymphocytes generated in vitro from embryonic stem cells. (2/205)

To study molecular events involved in B lymphocyte development and V(D)J rearrangement, we have established an efficient system for the differentiation of embryonic stem (ES) cells into mature Ig-secreting B lymphocytes. Here, we show that B lineage cells generated in vitro from ES cells are functionally analogous to normal fetal liver-derived or bone marrow-derived B lineage cells at three important developmental stages: first, they respond to Flt-3 ligand during an early lymphopoietic progenitor stage; second, they become targets for Abelson murine leukemia virus (A-MuLV) infection at a pre-B cell stage; third, they secrete Ig upon stimulation with lipopolysaccharide at a mature mitogen-responsive stage. Moreover, the ES cell-derived A-MuLV-transformed pre-B (EAB) cells are phenotypically and functionally indistinguishable from standard A-MuLV-transformed pre-B cells derived from infection of mouse fetal liver or bone marrow. Notably, EAB cells possess functional V(D)J recombinase activity. In particular, the generation of A-MuLV transformants from ES cells will provide an advantageous system to investigate genetic modifications that will help to elucidate molecular mechanisms in V(D)J recombination and in A-MuLV-mediated transformation.  (+info)

Drosophila abelson interacting protein (dAbi) is a positive regulator of abelson tyrosine kinase activity. (3/205)

Human and mouse Abelson interacting proteins (Abi) are SH3-domain containing proteins that bind to the proline-rich motifs of the Abelson protein tyrosine kinase. We report a new member of this gene family, a Drosophila Abi (dAbi) that is a substrate for Abl kinase and that co-immunoprecipitates with Abl if the Abi SH3 domain is intact. We have identified a new function for both dAbi and human Abi-2 (hAbi-2). Both proteins activate the kinase activity of Abl as assayed by phosphorylation of the Drosophila Enabled (Ena) protein. Removal of the dAbi SH3 domain eliminates dAbi's activation of Abl kinase activity. dAbi is an unstable protein in cells and is present at low steady state levels but its protein level is increased coincident with phosphorylation by Abl kinase. Expression of the antisense strand of dAbi reduces dAbi protein levels and abolishes activation of Abl kinase activity. Modulation of Abi protein levels may be an important mechanism for regulating the level of Abl kinase activity in the cell.  (+info)

Inhibition of v-Abl transformation by p53 and p19ARF. (4/205)

Tumorigenesis is a multistep process that involves the activation of oncogenes and the inactivation of tumor suppressor genes. The transforming activity of the v-Abl oncogene of Abelson murine leukemia virus (A-MuLV) in immortal cell lines has been well studied, while the effects of v-Abl in primary fibroblasts are less clear. Here we show that v-Abl causes cell cycle arrest in primary mouse embryonic fibroblasts (MEFs) and elevated levels of both p53 and the cyclin-dependent kinase inhibitor p21Cip. p53-/- or p19ARF-/- MEFs were resistant to v-Abl-induced cell cycle arrest. Although wild-type MEFs were resistant to v-Abl transforming activity, p53-/- or p19ARF-/- MEFs were susceptible. The results indicate that loss of p19ARF and p53 function plays an important role during the transformation of primary cells by v-Abl. We suggest that although v-Abl is a potent oncogene, its full potential transforming activity cannot be realized until the ARF-, and p53-dependent growth inhibitory pathway is disabled. We also show that p53 is not the mediator of v-Abl toxicity in immortal fibroblasts and does not determine the susceptibility of immortal fibroblasts to v-Abl transformation.  (+info)

The carboxyl terminus of v-Abl protein can augment SH2 domain function. (5/205)

Abelson murine leukemia virus (Ab-MLV) transforms NIH 3T3 and pre-B cells via expression of the v-Abl tyrosine kinase. Although the enzymatic activity of this molecule is absolutely required for transformation, other regions of the protein are also important for this response. Among these are the SH2 domain, involved in phosphotyrosine-dependent protein-protein interactions, and the long carboxyl terminus, which plays an important role in transformation of hematopoietic cells. Important signals are sent from each of these regions, and transformation is most likely orchestrated by the concerted action of these different parts of the protein. To explore this idea, we compared the ability of the v-Src SH2 domain to substitute for that of v-Abl in the full-length P120 v-Abl protein and in P70 v-Abl, a protein that lacks the carboxyl terminus characteristic of Abl family members. Ab-MLV strains expressing P70/S2 failed to transform NIH 3T3 cells and demonstrated a greatly reduced capacity to mediate signaling events associated with the Ras-dependent mitogen-activated protein (MAP) kinase pathway. In contrast, Ab-MLV strains expressing P120/S2 were indistinguishable from P120 with respect to these features. Analyses of additional mutants demonstrated that the last 162 amino acids of the carboxyl terminus were sufficient to restore transformation. These data demonstrate that an SH2 domain with v-Abl substrate specificity is required for NIH 3T3 transformation in the absence of the carboxyl terminus and suggest that cooperativity between the extreme carboxyl terminus and the SH2 domain facilitates the transmission of transforming signals via the MAP kinase pathway.  (+info)

Transgenic human lambda 5 rescues the murine lambda 5 nullizygous phenotype. (6/205)

The human lambda 5 (hu lambda 5) gene is the structural homologue of the murine lambda 5 (m lambda 5) gene and is transcriptionally active in pro-B and pre-B lymphocytes. The lambda 5 and VpreB polypeptides together with the Ig mu H chain and the signal-transducing subunits, Ig alpha and Ig beta, comprise the pre-B cell receptor. To further investigate the pro-B/pre-B-specific transcription regulation of hu lambda 5 in an in vivo model, we generated mouse lines that contain a 28-kb genomic fragment encompassing the entire hu lambda 5 gene. High levels of expression of the transgenic hu lambda 5 gene were detected in bone marrow pro-B and pre-B cells at the mRNA and protein levels, suggesting that the 28-kb transgene fragment contains all the transcriptional elements necessary for the stage-specific B progenitor expression of hu lambda 5. Flow cytometric and immunoprecipitation analyses of bone marrow cells and Abelson murine leukemia virus-transformed pre-B cell lines revealed the hu lambda 5 polypeptide on the cell surface and in association with mouse Ig mu and mouse VpreB. Finally, we found that the hu lambda 5 transgene is able to rescue the pre-B lymphocyte block when bred onto the m lambda 5-/- background. Therefore, we conclude that the hu lambda 5 polypeptide can biochemically and functionally substitute for m lambda 5 in vivo in pre-B lymphocyte differentiation and proliferation. These studies on the mouse and human pre-B cell receptor provide a model system to investigate some of the molecular requirements necessary for B cell development.  (+info)

Activation of V(D)J recombination induces the formation of interlocus joints and hybrid joints in scid pre-B-cell lines. (7/205)

V(D)J recombination is the mechanism by which antigen receptor genes are assembled. The site-specific cleavage mediated by RAG1 and RAG2 proteins generates two types of double-strand DNA breaks: blunt signal ends and covalently sealed hairpin coding ends. Although these DNA breaks are mainly resolved into coding joints and signal joints, they can participate in a nonstandard joining process, forming hybrid and open/shut joints that link coding ends to signal ends. In addition, the broken DNA molecules excised from different receptor gene loci could potentially be joined to generate interlocus joints. The interlocus recombination process may contribute to the translocation between antigen receptor genes and oncogenes, leading to malignant transformation of lymphocytes. To investigate the underlying mechanisms of these nonstandard recombination events, we took advantage of recombination-inducible cell lines derived from scid homozygous (s/s) and scid heterozygous (s/+) mice by transforming B-cell precursors with a temperature-sensitive Abelson murine leukemia virus mutant (ts-Ab-MLV). We can manipulate the level of recombination cleavage and end resolution by altering the cell culture temperature. By analyzing various recombination products in scid and s/+ ts-Ab-MLV transformants, we report in this study that scid cells make higher levels of interlocus and hybrid joints than their normal counterparts. These joints arise concurrently with the formation of intralocus joints, as well as with the appearance of opened coding ends. The junctions of these joining products exhibit excessive nucleotide deletions, a characteristic of scid coding joints. These data suggest that an inability of scid cells to promptly resolve their recombination ends exposes the ends to a random joining process, which can conceivably lead to chromosomal translocations.  (+info)

Loss of heterozygosity at the Ink4a/Arf locus facilitates Abelson virus transformation of pre-B cells. (8/205)

In many tumor systems, analysis of cells for loss of heterozygosity (LOH) has helped to clarify the role of tumor suppressor genes in oncogenesis. Two important tumor suppressor genes, p53 and the Ink4a/Arf locus, play central roles in the multistep process of Abelson murine leukemia virus (Ab-MLV) transformation. p53 and the p53 regulatory protein, p19Arf, are required for the apoptotic crisis that characterizes the progression of primary transformed pre-B cells to fully malignant cell lines. To search for other tumor suppressor genes which may be involved in the Ab-MLV transformation process, we used endogenous proviral markers and simple-sequence length polymorphism analysis to screen Abelson virus-transformed pre-B cells for evidence of LOH. Our survey reinforces the role of the p53-p19 regulatory pathway in transformation; 6 of 58 cell lines tested had lost sequences on mouse chromosome 4, including the Ink4a/Arf locus. Consistent with this pattern, a high frequency of primary pre-B-cell transformants derived from Ink4a/Arf +/- mice became established cell lines. In addition, half of them retained the single copy of the locus when the transformation process was complete. These data demonstrate that a single copy of the Ink4a/Arf locus is not sufficient to fully mediate the effects of these genes on transformation.  (+info)

TY - JOUR. T1 - Synthesis and Evaluation of Multisubstrate Inhibitors of an Oncogene-Encoded Tyrosine-Specific Protein Kinase.. AU - Kruse, Carolyn H.. AU - Holden, Kenneth G.. AU - Lynn Pritchard, M.. AU - Feild, John A.. AU - Rieman, David J.. AU - Greig, Russell G.. AU - Poste, George. PY - 1988/9/1. Y1 - 1988/9/1. N2 - The synthesis and testing of potential multisubstrate inhibitors of tyrosine-specific protein kinases are described. One of the substrates, ATP, was mimicked by the known kinase inhibitor 5′-[4-(fluorosulfonyl)benzoyl]adenosine, which was covalently linked via the sulfonyl moiety to tyrosine mimics. The resulting multisubstrate inhibitors were tested for their ability to inhibit the transfer of phosphate from ATP to a protein acceptor by p60v-abl the tyrosine kinase encoded by the transforming gene (v-abl) of the Abelson murine leukemia virus (A-MuLV). Although the series of inhibitors displayed moderately potent activity (IC50 values as low as 19 μM), the absence of large ...
Kanda, N. and Schreck, R. and Alt, F. et al. (1983) Isolation of amplified DNA sequences from IMR-32 human neuroblastoma cells: Facilitation by fluorescence-activated flow sorting of metaphase chromosomes. Proceedings of the National Academy of Sciences of the United States of America, 80 (13). pp. 4069-4073. ISSN 0027-8424. PMCID PMC394202. https://resolver.caltech.edu/CaltechAUTHORS:KANpnas83 Latt, Samuel A. and Goff, Stephen P. and Tabin, Clifford J. et al. (1983) Cloning and analysis of reverse transcript P160 genomes of Abelson murine leukemia virus. Journal of Virology, 45 (3). pp. 1195-1199. ISSN 0022-538X. https://resolver.caltech.edu/CaltechAUTHORS:LATjvir83 ...
Schlissel, Mark S. and Corcoran, Lynn M. and Baltimore, David (1991) Virus-transformed pre-B cells show ordered activation but not inactivation of immunoglobulin gene rearrangement and transcription. Journal of Experimental Medicine, 173 (3). pp. 711-720. ISSN 0022-1007. https://resolver.caltech.edu/CaltechAUTHORS:SCHLjem91 ...
Among several tyrosine-protein kinases, only v-abl could abrogate interleukin 3 dependence of a lymphoblastoid cell line; v-src and v-fps proteins gave partial or no interleukin 3 independence, respectively. Lymphokine independence was achieved via a nonautocrine mechanism. Direct involvement of c-myc in this process was not evident. ...
ABL2 antibody (v-abl Abelson murine leukemia viral oncogene homolog 2) for WB. Anti-ABL2 pAb (GTX81937) is tested in Human samples. 100% Ab-Assurance.
Brain tumors frequently recur or progress as focal masses after treatment with ionizing radiation. However, the mechanisms underlying the repopulation of tumor cells after radiation have remained unclear. In this study, we show that cellular signaling from Abelson murine leukemia viral oncogene homolog (Abl) to protein kinase Cδ (PKCδ) is crucial for fractionated-radiation-induced expansion of glioma-initiating cell populations and acquisition of resistance to anticancer treatments. Treatment of human glioma cells with fractionated radiation increased Abl and PKCδ activity, expanded the CD133-positive (CD133+) cell population that possesses tumor-initiating potential and induced expression of glioma stem cell markers and self-renewal-related proteins. Moreover, cells treated with fractionated radiation were resistant to anticancer treatments. Small interfering RNA (siRNA)-mediated knockdown of PKCδ expression blocked fractionated-radiation-induced CD133+ cell expansion and suppressed ...
Abl Protein Tyrosine Kinase (AbI) is a truncated form of the v-AbI Protein Tyrosine Kinase, a partner in the Gag-Abl fusion protein of the Abelson murine leukemia virus. Abl contains 407 amino acids (residues 237-643 of the p120-gag-abl polyprotein)
Looking for online definition of Abelson, Herbert T. in the Medical Dictionary? Abelson, Herbert T. explanation free. What is Abelson, Herbert T.? Meaning of Abelson, Herbert T. medical term. What does Abelson, Herbert T. mean?
Reed Abelson has been a reporter for The New York Times since 1995. She currently covers the business of health care, focusing on health insurance and how financial incentives affect the delivery of medical care. She witnessed the Affordable Care Act become law and is actively keeping an eye on what happens next. Before she began covering health care in 2002, Ms. Abelson covered a broad range of topics, from the collapse of Enron to the oversight of charitable organizations to accounting to personal investing. Before joining The Times, Ms. Abelson was a staff writer for Smart Money from 1993 to 1995, where she wrote in-depth investing features. From 1990 to 1993, she was a reporter for Forbes, where she profiled public and private companies. She began her journalism career as a reporter at the Philadelphia Business Journal, where she covered health care, venture capital, technology and the ports of Philadelphia. She graduated cum laude from Bryn Mawr College in 1983 with an A.B. in English
Branched-Chain Amino Acids: Pt. B (John N. Abelson, Melvin I. Simon, John R. Sokatch, Robert Adron Harris) pe OKIAN.ro. Pret: 702.99 lei. Volume 324 of Met
Sedimentation on the ocean floor; Rates of geochemical reactions at low temperatures and pressures; Geochemical indicators of marine and fresh-water sediments; Geochemical prospecting; Geochemistry of organic substances; Some chemical aspects of petroleum genesis; Mineral assemblages of the green river formation ...
During my time as CEO of Park Nicollet, I encouraged team members to call upon personal experiences to inspire constant improvement and lasting success ...
Cells and virus preparation. Transformed fetal liver cells, bone marrow cells, and tumor-derived cell lines were maintained in RPMI medium containing 10% FCS, 100 U/ml penicillin/streptomycin, and 5 μM β-mercaptoethanol. A010 cells that produce an ecotropic replication-deficient form of the Abelson virus were maintained in DMEM containing 10% FCS and 100 U/ml penicillin/streptomycin. Viral supernatant was prepared as previously described (49).. Mice and infection of neonatal mice with A-MuLV. TYK2-/- mice were described previously (25) and were backcrossed to C57BL/6 for 8 generations. Nude mice were purchased from the Institut für Labortierkunde und Genetik (Himberg, Austria).. For the infection of newborn animals, TYK2+/- were bred with TYK2-/- animals. Hence, every litter contained TYK2+/- and TYK2-/- animals to ensure appropriate controls. We initially started the in vivo transformation experiments by intercrossing TYK2+/- and TYK2+/- mice and analyzed leukemia/lymphoma formation in all 3 ...
Living with cerebral palsy? The personal injury lawyer at Abelson Personal Injury Law Firm in Washington, DC can help you file a claim.
The apparent enhancement of 2-deoxy-D-glucose uptake by mouse 3T3 cells accompanying transformation by SV40 virus is not due primarily to an effect on the transport process but to enhanced phosphorylation of the sugar by intracellular kinases. Moreover, the effect is not specifically a function of the presence of the viral genome, but is a reflection of the overall growth rate and physiological state of the cell. ...
Best-selling author, Dr. Brian Abelson, is the Clinical Director of Kinetic Health, an innovative health care facility that places a special focus on getting people back into an active, pain-free lifestyle. Dr. Abelson believes in doing everything he can to achieve the best results possible for his patients. This includes: Attending advanced courses every year to further his expertise in treating musculoskeletal conditions. Traveling to different regions of the world in search of new ways to achieve what most practitioners would consider an already extremely impressive track record. His clinical practice is in Calgary Alberta. Kinetic Health 403-241- ...
Personal Injury Lawyer Washington DC Testimonial If you find that you are in need of a personal injury lawyer, I HIGHLY recommend Attorney Michael Abelson. I suffered a permanent disabling injury in a car accident. Mr. Abelson worked hard on my case and prepared carefully for trial. He guided me every step along the way. His diligent preparation led to a settlement agreement that greatly exceeded my expectation. Written by: David Kentley Date published: 09/07/2018 ...
Primary cultures of cells tend to have a limited life span, which in turn limits the availability of a consistent population of cells to study
Gomes, Paulo J; Ousler, George W; Welch, Donna L; Smith, Lisa M; Coderre, Jeffrey; Abelson, Mark B (Clinical Ophthalmology (Auckland, N.Z.), 2013) Link to Published Version ...
Alan Abelson in this weekends Barrons: Sergey Aleynikov … recently left Goldman … (&) took with him the code to the firms proprietary software for so-called high-frequency trading. … Critics claim that via its special software, Goldman is able to detect huge order positions ahead of their execution, and to profit accordingly; in other words, that high-frequency trading is essentially high-tech front-running. … The hubbub over high-frequency trading grew in intensity and virulence when a slightly befuddled prosecutor told the court, in arguing to keep Mr. Aleynikov in the hoosegow rather than allowing him to make bail, the Goldman code in the wrong hands could lead to unfair manipulation of markets. We couldnt help being amused by how ravenously the malapropism was seized upon by hordes of cybernauts, unable to resist the obvious, as suggesting that in Goldmans hands it was used for fair manipulation. Wed be remiss in not crediting Tyler Durden and his feisty ...
Eckner, R J. and Steeves, R A., Classification of the murine leukemia viruses. Pseudotype neutrali- zation by specific antisera. Abstr. (1971). Subject Strain Bibliography 1971. 2412 ...
Sibal, L R.; Hollis, V W.; and Fink, M A., Detection of antigenic subunits of a murine leukemia virus by passive hemagglutination-inhibition. (1971). Subject Strain Bibliography 1971. 339 ...
Best-selling author, Dr. Brian Abelson, is the Clinical Director of Kinetic Health, an innovative health care facility that places a special focus on getting people back into an active, pain-free lifestyle. Dr. Abelson believes in doing everything he can to achieve the best results possible for his patients. This includes: Attending advanced courses every year to further his expertise in treating musculoskeletal conditions. Traveling to different regions of the world in search of new ways to achieve what most practitioners would consider an already extremely impressive track record. His clinical practice is in Calgary Alberta. Kinetic Health 403-241- ...
Discovering a genetic mutation is not a typical fantasy, but for Jesse Abelson, a former post-baccalaureate student at Yale, it was - and it recently became a reality that has led to advances in the study of Tourettes Syndrome.
Abelson, R. P. (1995). Statistics as principled argument. Hillsdale, NJ: Lawrence Erlbaum Press. Bain, Ken and James Zimmerman, "Understanding Great Teaching," Peer Review, Spring 2009, Vol. 11, No. 2. ...
One big hospital, St. Lukes, has come to dominate the health-care marketplace in Boise, Idaho, according to an article in The New York Times by Julie Creswell and Reed Abelson, and other cities and regions are experiencing similar consolidations. A little over half of the 1,400 doctors in southwestern Idaho are employed by St. Lukes […]. ...
CONTEXT: Cushing disease (CD) is a rare disorder with severe sequels and incompletely understood pathogenesis. The underlying corticotroph adenomas harbor frequently somatic mutations in the ubiquitin-specific peptidase 8 (USP8) gene. These mutations render USP8 hyperactive and prevent client proteins from degradation.. OBJECTIVE: To investigate the impact of USP8 mutations on proteins deregulated in CD.. DESIGN: One hundred eight pituitary adenomas (75 corticotroph [58 USP8 wild type (WT) and 17 USP8 mutated], 14 somatotroph, and 19 nonfunctioning) were investigated by immunohistochemistry. All evaluated proteins [USP8, arginine vasopressin receptor 1b and 2, corticotropin-releasing hormone receptor, cAMP response element-binding protein (CREB), p27/kip1, cyclin E, heat shock protein 90 (HSP90), orphan nuclear receptor 4, epidermal growth factor receptor, histone deacetylase 2, glucocorticoid receptor, cyclin-dependent kinase 5 and Abelson murine leukemia viral oncogene homolog 1 enzyme ...
Hybrid Moloney/Amphotropic murine leukemia virus (Mo/A-MuLv) ATCC ® VR-1450™ Designation: 4070A envelope strain Application: Analytical methodologies Pharmaceutical and Personal Care
But, we can ask whether the atmosphere proposed by Miller was likely to be stable. Abelson reports that the ammonia in the atmosphere would have decomposed within 30,000 years: it is inherently unstable, decomposing into nitrogen and hydrogen. Also, much of it would dissolve out of the atmosphere due to its great solubility in water. Methane would only have lasted for about 1% of the time required for the appearance of life by this process, according to Shimzu. Brinkman has shown that even the water vapour would have been broken down due to the suns radiation. The trouble is that we think of these gases as stable - indeed they are relative to our lifetime, but not on the evolutionary timescales. And hydrogen? We know that hydrogen does not exist as an element on this planet: it escapes into space very rapidly due to its low density ...
BioAssay record AID 152712 submitted by ChEMBL: Concentration which leads to inhibitory effect on the proliferation of murine leukemia (P388) cells.
The Murine Leukemia Virus (MLV) is a gammaretrovirus that hijack host components of the endosomal sorting complex required for transport (ESCRT) for budding. To determine the minimal requirements for ESCRT factors in MLV viral and viral-like particles (VLP) release, an siRNA knockdown screen of ESCRT(-associated) proteins was performed in MLV-producing human cells. We found that MLV VLPs and virions primarily engage the ESCRT-I factor Tsg101 and marginally the ESCRT-associated adaptors Nedd4-1 and Alix to enter the ESCRT pathway. Conversely, the inactivation of ESCRT-II had no impact on VLP and virion egress. By analyzing the effects of individual ESCRT-III knockdowns, VLP and virion release was profoundly inhibited in CHMP2A- and CHMP4B-knockdown cells. In contrast, neither the CHMP2B and CHMP4A isoforms nor CHMP3, CHMP5, and CHMP6 were found to be essential. In case of CHMP1, we unexpectedly observed that the CHMP1A isoform was specifically required for virus budding, but dispensable for VLP release.
Glycoprotein, Glycoproteins, Human, Infection, Somatostatin, Virus, Binding Site, Gene, Leukemia, Moloney Murine Leukemia Virus, Mouse, Murine Leukemia Virus, Somatostatin Receptors, Stomatitis, Vesicular Stomatitis, Cell, Cell Lines, Cholesterol, Complement, Cytoplasm
The biomaterial transformation process is defined as an event with one or more biomaterials as inputs and outputs. For example, DNA extraction from a blood sample is a biomaterial transformation process, where blood is the input biological material, DNA is the output material and the DNA extraction reagents and devices used in the process are other participants. An assay is a planned process with the objective to produce information about some evaluant (ID: obo:OBI_0000070) [8]. It has biological material as input and data as output. For example, a microarray based genotyping assay has DNA as input and raw image data as output, where reagents, instruments and software utilized in the process are other participants. Starting with the raw data generated from the assay, we move to the data transformation processes. A data transformation process is a protocol application that produces output data from input data (ID: obo:OBI_0200000) [8].. With the application of OBI concepts in MIGen, ...
In June, Macareno and his posse, all Class of 2020, get off Amtraks Acela Express train and take a shuttle bus to Brandeis for science boot camp. On the first day, they gather in a classroom in the Abelson physics building, the air conditioner cranked so high everyone is shivering. (Science buildings are notoriously cold, the students will learn.) Some of them already own Brandeis sweatshirts and water bottles, and they bring along the scientific calculators they were given the day before.. Kosinski-Collins, who earned a PhD at MIT, tells them college science is profoundly different from high-school science. With equal parts candor and caring, she sets high expectations, describing the intense workload. The students know that they will be held to lofty standards and that she will support them.. Later in the day, they gather around a long lab table in the Shapiro Science Center, in an area Kosinski-Collins calls Hufflepuff - a nod to one of the houses at Harry Potters Hogwarts School. An array ...
has also worked in computer languages, in computer architecture and in VLSI design.. Sussman is a coauthor (with Hal Abelson and Julie Sussman) of the introductory computer science textbook used at M.I.T. for 23 years. The textbook, ``Structure and Interpretation of Computer Programs, has been translated into French, German, Chinese, Polish, Japanese, and Korean. As a result of this and other contributions to computer-science education, Sussman received the ACMs Karl Karlstrom Outstanding Educator Award in 1990, and the Amar G. Bose award for teaching in 1992.. Sussmans contributions to Artificial Intelligence include problem solving by debugging almost-right plans, propagation of constraints applied to electrical circuit analysis and synthesis, dependency-based explanation and dependency-based backtracking, and various language structures for expressing problem-solving strategies. Sussman and his former student Guy L. Steele Jr. invented the Scheme programming language in 1975.. Sussman saw ...
Our goal is to understand the principles of Perception, Action and Learning in autonomous systems that successfully interact with complex environments and to use this understanding to design future systems.
"President's Cancer Panel". Witte ON, Dasgupta A, Baltimore D (February 28, 1980). "Abelson murine leukemia virus protein is ... Konopka JB, Watanabe SM, Witte ON (July 1984). "An alteration of the human c-abl protein in K562 leukemia cells unmasks ... Witte's research has contributed to the understanding of human leukemias, immune disorders and stem cell activity in cancers of ... This finding influenced the development of targeted drugs like Ibrutinib to treat leukemia and lymphoma. Witte's current ...
... fibroblasts by cloned Abelson murine leukemia virus DNA and recovery of transmissible virus by recombination with helper virus ... In Weinberg's lab, Tabin constructed murine leukemia virus, the first recombinant retrovirus that could be used as a eukaryotic ... "Human EJ bladder carcinoma oncogene is homologue of Harvey sarcoma virus ras gene". Nature. 297 (5866): 474-8. Bibcode: ...
... the mineral Abelson murine leukemia virus, also known as "Abelson's virus" Abelson's paradox This page lists people with the ... American journalist Evelyn Abelson (1886-1967), English artist Hal Abelson, American computer scientist Hope Abelson (1910-2006 ... American biochemist Matthew Abelson, American musician Neva Abelson (1910-2000), American physician Philip Abelson (1913-2004 ... Abelson, originating from both Swedish and Yiddish, and derived from the name Abel, is the surname of: Alan Abelson (1925-2013 ...
Abelson murine leukemia virus Bovine leukemia virus Feline leukemia virus Human T-lymphotropic virus Murine leukemia virus ... Xenotropic murine leukemia virus-related virus Gibbon-ape leukemia virus Leucosis This disambiguation page lists articles ... associated with the title Leukemia virus. If an internal link led you here, you may wish to change the link to point directly ...
The Moloney, Rauscher, Abelson and Friend MLVs, named for their discoverers, are used in cancer research. Endogenous MLVs are ... The Friend virus (FV) is a strain of murine leukemia virus. The Friend virus has been used for both immunotherapy and vaccines ... The murine leukemia viruses (MLVs or MuLVs) are retroviruses named for their ability to cause cancer in murine (mouse) hosts. ... The murine leukemia viruses are group/type VI retroviruses belonging to the gammaretroviral genus of the Retroviridae family. ...
Murine leukemia virus. *Abelson murine leukemia virus. *Friend virus. *Feline leukemia virus ... dsDNA-RT viruses are the seventh group in the Baltimore virus classification. They are not considered DNA viruses (class I of ... Khan, Jawaid A.; Dijkstra, Jeanne (2002). Plant viruses as molecular pathogens. Psychology Press. pp. 317-. ISBN 978-1-56022- ... Retrieved from "https://en.wikipedia.org/w/index.php?title=DsDNA-RT_virus&oldid=748922731" ...
Talk:Abadina virus. *Talk:Abalone shriveling syndrome-associated virus. *Talk:Abelson murine leukemia virus ... Pages in category "Low-importance virus articles". The following 200 pages are in this category, out of approximately 1,493 ... Retrieved from "https://en.wikipedia.org/w/index.php?title=Category:Low-importance_virus_articles&oldid=388350164" ...
Murine leukemia virus. *Abelson murine leukemia virus. *Friend virus. *Feline leukemia virus ... Xenotropic Murine Leukemia Virus-Related Virus: Classification[citation needed] Xenotropic viruses (xenos Gr. foreign; tropos ... Xenotropic murine leukemia virus-related virus (XMRV) is a retrovirus which was first described in 2006 as an apparently novel ... "XMRV (Xenotropic Murine Leukemia Virus-related Virus) , CDC". www.cdc.gov. Retrieved 2018-04-17.. .mw-parser-output cite. ...
Murine leukemia virus. *Abelson murine leukemia virus. *Friend virus. *Feline leukemia virus ... Murine leukemia virus; others include Feline leukemia virus. *Genus Deltaretrovirus; type species: Bovine leukemia virus; ... Feline leukemia virus and Feline immunodeficiency virus infections are treated with biologics, including the only ... Such viruses are either single stranded RNA (e.g. HIV) or double stranded DNA (e.g. Hepatitis B virus) viruses. ...
... leukemia virus, murine MeSH B04.820.650.375.525.020 - abelson murine leukemia virus MeSH B04.820.650.375.525.050 - akr murine ... leukemia virus, murine MeSH B04.909.574.807.375.525.020 - abelson murine leukemia virus MeSH B04.909.574.807.375.525.050 - akr ... leukemia virus, murine MeSH B04.909.777.731.375.525.020 - abelson murine leukemia virus MeSH B04.909.777.731.375.525.050 - akr ... leukemia virus MeSH B04.820.650.375.525.225 - friend murine leukemia virus MeSH B04.820.650.375.525.300 - gross virus MeSH ...
Murine leukemia virus. *Abelson murine leukemia virus. *Friend virus. *Feline leukemia virus ... Murine leukemia virus; others include Feline leukemia virus. *Genus Deltaretrovirus; type species: Bovine leukemia virus; ... Feline leukemia virus and Feline immunodeficiency virus infections are treated with biologics, including the only ... "Virus Taxonomy: 2018b Release" (html). International Committee on Taxonomy of Viruses (ICTV). March 2019. Retrieved 16 March ...
Murine leukemia virus. *Abelson murine leukemia virus. *Friend virus. *Feline leukemia virus ... Caprine arthritis encephalitis virus. Visna/maedi virus. Primate lentivirus group. Human immunodeficiency virus 1. Human ... Bovine immunodeficiency virus. Jembrana disease virus. Equine lentivirus group. Equine infectious anemia virus. Feline ... Simian immunodeficiency virus. Gray mouse lemur prosimian immunodeficiency virus (pSIVgml). Lentivirus (lente-, Latin for "slow ...
The symbol ABL is derived from Abelson, the name of a leukemia virus which carries a similar protein. The symbol BCR is derived ... Ras in particular is shown to be an important downstream target of BCR-ABL1 in CML, as Ras mutants in murine models disrupt the ... Chronic myelogenous leukemia. References[edit]. *^ a b c Wapner J. The Philadelphia Chromosome: A Genetic Mystery, a Lethal ... Proliferative roles in leukemia[edit]. The BCR-ABL1 fusion gene and protein encoded by the Philadelphia chromosome affects ...
LEUKEMIA VIRUS, MURINE) capable of transforming lymphoid cells and producing a rapidly progressing lymphoid leukemia after ... superinfection with FRIEND MURINE LEUKEMIA VIRUS; MOLONEY MURINE LEUKEMIA VIRUS; or RAUSCHER VIRUS. ... A replication-defective strain of Murine leukemia virus ( ... Abelson murine leukemia virus. Known as: Abelson leukemia virus ... Abelsons virus, A-MuLV Expand. A replication-defective strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) capable of ...
Corrective recombination of mouse immunoglobulin kappa alleles in Abelson murine leukemia virus-transformed pre-B cells.. R M ... Corrective recombination of mouse immunoglobulin kappa alleles in Abelson murine leukemia virus-transformed pre-B cells. ... Corrective recombination of mouse immunoglobulin kappa alleles in Abelson murine leukemia virus-transformed pre-B cells. ... Corrective recombination of mouse immunoglobulin kappa alleles in Abelson murine leukemia virus-transformed pre-B cells. ...
Antisera reactive with the Abelson murine leukemia virus (A-MuLV)-specified P120 (anti-AbT sera) were produced in C57L/J mice. ... Preparation of Syngeneic Tumor Regressor Serum Reactive with the Unique Determinants of the Abelson Murine Leukemia Virus- ... Preparation of syngeneic tumor regressor serum reactive with the unique determinants of the Abelson murine leukemia virus- ... Preparation of Syngeneic Tumor Regressor Serum Reactive with the Unique Determinants of the Abelson Murine Leukemia Virus- ...
After purification to homogeneity, Abelson virus-induced TGF migrates as a single polypeptide with an apparent size of 7400 ... Transformation-defective mutants of Abelson MuLV do not transform cells, do not have their virus coded transforming gene ... Abelson MuLV-induced TGF morphologically transforms cells in culture, competes with 125I-labeled epidermal growth factor (EGF) ... is correlated with a tyrosine-specific protein kinase that is functionally active and is associated with the major Abelson MuLV ...
The Abelson murine leukemia virus (Ab-MLV, A-MuLV) is a retrovirus (Class VI) used to induce transformation of murine lymphoid ... The Abelson murine leukemia virus is named for the American pediatrician Herbert T. Abelson who first described and isolated it ... A highly efficient helper virus commonly used when growing A-MuLV in vitro is Moloney murine leukemia virus (M-MuLV). It causes ... A-MuLV causes a rapidly progressive lymphosarcoma known as Abelson disease in mice, which is a type of leukemia that does not ...
"Presidents Cancer Panel". Witte ON, Dasgupta A, Baltimore D (February 28, 1980). "Abelson murine leukemia virus protein is ... Konopka JB, Watanabe SM, Witte ON (July 1984). "An alteration of the human c-abl protein in K562 leukemia cells unmasks ... Wittes research has contributed to the understanding of human leukemias, immune disorders and stem cell activity in cancers of ... This finding influenced the development of targeted drugs like Ibrutinib to treat leukemia and lymphoma. Wittes current ...
Characterization of flat revertants isolated from cells transformed by Abelson murine leukemia virus (Ab-MuLV). In: Journal of ... Characterization of flat revertants isolated from cells transformed by Abelson murine leukemia virus (Ab-MuLV). / Oka, Takashi ... N2 - Transformed Fisher rat fibroblast cell lines by Abelson murine leukemia virus frequently revert to the normal phenotype in ... AB - Transformed Fisher rat fibroblast cell lines by Abelson murine leukemia virus frequently revert to the normal phenotype in ...
The Abelson murine leukemia virus (A-MuLV)-transformed lymphoid cell line L1-2 is exceptional since it is rejected following ... abstract = "The Abelson murine leukemia virus (A-MuLV)-transformed lymphoid cell line L1-2 is exceptional since it is rejected ... N2 - The Abelson murine leukemia virus (A-MuLV)-transformed lymphoid cell line L1-2 is exceptional since it is rejected ... AB - The Abelson murine leukemia virus (A-MuLV)-transformed lymphoid cell line L1-2 is exceptional since it is rejected ...
The single exposure to the Abelson murine leukemia virus (A-MuLV) induces a slowly evolving B lymphoid leukemia/lymphoma that ... Nonstandard abbreviations used: A-MuLV, Abelson murine leukemia virus; EC50, 50% effective concentration; MTT, 3-(4,5- ... The cell-autonomous properties of Abelson murine leukemia virus-transformed (A-MuLV-transformed) TYK2-/- cells were unaltered, ... Mice deficient in TYK2 developed Abelson-induced B lymphoid leukemia/lymphoma as well as TEL-JAK2-induced T lymphoid leukemia ...
Cell Type: Macrophage; Abelson Murine Leukemia Virus Transformed .plsearchwithin .searchinresults input.text { padding: 7px 4px ... Tissue: Abelson Murine Leukemia Virus-Induced Tumor; Ascites Disease Abelson Murine Leukemia Virus-Induced Tumor ...
Abelson Murine Leukemia Virus. A replication-defective strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) capable of ... Friend Murine Leukemia Virus. A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) producing leukemia of the reticulum- ... Moloney Murine Leukemia Virus. A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) arising during the propagation of S37 ... lymphoid cells and producing a rapidly progressing lymphoid leukemia after superinfection with FRIEND MURINE LEUKEMIA VIRUS; ...
The investigators hope to learn more about the clinical efficacy of bortezomib in T-cell prolymphocytic leukemia. Patients ... ... Abelson Murine Leukemia Virus. A replication-defective strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) capable of ... Friend Murine Leukemia Virus. A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) producing leukemia of the reticulum- ... Moloney Murine Leukemia Virus. A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) arising during the propagation of S37 ...
... fibroblasts by cloned Abelson murine leukemia virus DNA and recovery of transmissible virus by recombination with helper virus ... In Weinbergs lab, Tabin constructed murine leukemia virus, the first recombinant retrovirus that could be used as a eukaryotic ... "Human EJ bladder carcinoma oncogene is homologue of Harvey sarcoma virus ras gene". Nature. 297 (5866): 474-8. Bibcode: ...
... a partner in the Gag-Abl fusion protein of the Abelson murine leukemia virus. Abl contains 407 amino acids (residues 237-643 of ... Abelson Murine Leukemia Virus, recombinant (E. coli). Product Source. Isolated from a strain of E. coli that carries the ... a partner in the Gag-Abl fusion protein of the Abelson murine leukemia virus. Abl contains 407 amino acids (residues 237-643 of ... truncated AbI Protein Kinase encoded by the Abelson murine leukemia virus under the control of a T7 expression system (kindly ...
Cook WD (1982) Rapid thymomas induced by Abelson murine leukemia virus. Proc Natl Acad Sci USA 79:2917-2921PubMedCrossRefGoogle ... Rous Sarcoma Virus Avian Myeloblastosis Virus Cellular Oncogene Avian Sarcoma Virus Murine Sarcoma Virus These keywords were ... Witte ON, Rosenberg N, Baltimore D (1979 b) A normal cell protein cross-reactive to the major Abelson murine leukemia virus ... Goff SP, Gilboa E, Witte ON, Baltimore D (1980) Structure of the Abelson murine leukemia virus genome and the homologous ...
The role of oncogene expression in tumor metastasis was examined using the Abelson leukemia virus-transformed murine large cell ... Rosenberg, N., andWitte, O. N., 1980, Abelson murine leukemia virus mutants with alteration in the virus specific p120 molecule ... Witte, O. N., Dasgupta, A., andBaltimore, D., 1980, Abelson murine leukemia virus protein is phosphorylatedin vitro to form ... Wang, J. Y. J., andBaltimore, D., 1983, Cellular RNA homologous to the Abelson murine leukemia virus transforming gene: ...
... gene into an Abelson murine leukemia virus-transformed lymphoid cell line. Plasmid pSV2gpt-κ41, containing the ĸ light chain ... We have introduced a functionally rearranged murine ĸ light chain immunoglobulin (Ig) ... Abelson murine leukemia virus; DNA transfection; gpt selection; lipopolysaccharide induction. Issue or Number:. 24. ...
He isolated the Abelson murine leukemia virus; identified the v-abl oncogene that is the cause of the viral cancer; and ... Goff has strategically and cleverly leveraged viruses to decipher how normal cells enable virus replication, yet defend against ... Goffs work led to realization that ABL is aberrantly expressed in chronic myelogenous leukemia in humans and led to the ... Goff developed the first methods to deliver recombinant DNA molecules into mammalian cells by employing SV40 virus, and was ...
Lane 3 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus). Lysates/proteins at 10 µg per lane. ... Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) lysate. Lane 2 : HEK293 (Human embryonic kidney). ... CSDE1/NRU was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell ...
... simian sarcoma virus, and feline sarcoma virus, respectively, have been determined by in situ molecular hybridization of 3H- ... the cellular counterparts of the v-onc genes of Abelson murine leukemia virus, ... the cellular counterparts of the v-onc genes of Abelson murine leukemia virus, simian sarcoma virus, and feline sarcoma virus, ... at 15q26.1 is also distal to the breakpoint in chromosome 15 in the translocation commonly seen in acute promyelocytic leukemia ...
Lane 4 : Raw264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate. Lysates/proteins at 10 µg per lane.. ... Lane 3 : Molt-4 (human acute lymphoblastic leukemia) whole cell lysate. Lane 4 : Human thymus tissue lysate. Lane 5 : Mouse ...
The Abelson leukemia virus-transformed murine Mac Raw 264.7 cells were purchase from ATCC (TIB-71). In vitro activation of M1 ... Raschke WC, Baird S, Ralph P, Nakoinz I. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell. 1978;15( ... In a recent genome-wide analysis of NOTCH1/CSL binding in lymphoblastic leukemia cells, only 36% of NICD1-binding events are ... analysis reveals conserved and divergent features of Notch1/RBPJ binding in human and murine T-lymphoblastic leukemia cells. ...
RAW 264.7 cell line slides ( mouse: Abelson murine leukemia virus - induced tumor ) from GeneTex. 7. C2C12 cell line slides ( ... L1210 cell line slides ( mouse: lymphocytic leukemia ) from GeneTex. 5. EL - 4 cell line slides ( mouse: T lymphocyte; lymphoma ... MOLT - 4 cell line slides ( human: T lymphoblast; acute lymphoblastic leukemia ) from GeneTex. 10. A - 549 cell line slides ( ...
... lymphocytic leukemia ) from GeneTex,Mouse L1210 cells were cultured in DMEM and harvested at the log phase of growth. In order ... RAW 264.7 cell line slides ( mouse: Abelson murine leukemia virus - induced tumor ) from GeneTex. 6. C2C12 cell line slides ( ... MOLT - 4 cell line slides ( human: T lymphoblast; acute lymphoblastic leukemia ) from GeneTex. 10. A - 549 cell line slides ( ...
RAW 264.7 cell line slides ( mouse: Abelson murine leukemia virus - induced tumor ) from GeneTex. 6. C2C12 cell line slides ( ... L1210 cell line slides ( mouse: lymphocytic leukemia ) from GeneTex. 4. EL - 4 cell line slides ( mouse: T lymphocyte; lymphoma ... MOLT - 4 cell line slides ( human: T lymphoblast; acute lymphoblastic leukemia ) from GeneTex. 10. A - 549 cell line slides ( ...
Thymic targets for Abelson murine leukemia virus are early gamma/delta T lymphocytes. Source:http://linkedlifedata.com/resource ...
Remove This Item Tissue/Cell Type: RAW 264.7 (Abelson murine leukemia virus-induced tumor) ...
c-abl is the normal cellular homolog of the v-abl transforming gene of Abelson murine leukemia virus. By constructing ... the v-abl oncogene of the Abelson murine leukemia virus by the replacement of their N-terminal sequences with viral gag ... Overexpression of p150c-abl type IV in a retroviral vector similar to Abelson virus does not transform NIH 3T3 fibroblasts, ... Our study unveiled an unexpected role of DBN1 in restricting the entry of RV and other viruses into host cells and more broadly ...
1976) A quantitative assay for transformation of bone marrow cells by Abelson murine leukemia virus. J. Exp. Med. 143:1453-1463 ... Abelson murine leukemia virus (Ab-MLV) encodes the v-Abl protein tyrosine kinase and induces transformation of immortalized ... Expression of the protein tyrosine kinase encoded by the v-abl oncogene of Abelson murine leukemia virus (Ab-MLV) induces ... 1990) Temperature-sensitive mutants of Abelson murine leukemia virus deficient in protein tyrosine kinase activity. J. Virol. ...
  • Photochemotherapy and Graft-versus-Leukemia Reaction in Acute Leukemia: Tumor Immunity and Survival Are Dependent on Timing of Photochemotherapy of the Skin. (bioportfolio.com)
  • Cure of acute leukemia after transplantation is mediated by the grafted cells. (bioportfolio.com)
  • Age Acts as an Adverse Independent Variable for Survival in Acute Lymphoblastic Leukemia: Data From a Cohort in Northeast Mexico. (bioportfolio.com)
  • Survival for acute lymphoblastic leukemia (ALL) decreases with age. (bioportfolio.com)
  • Icariin induces apoptosis in acute promyelocytic leukemia by targeting PIM1. (bioportfolio.com)
  • Acute promyelocytic leukemia (APL) is one type of acute myeloid leukemia (AML) featured by abnormal, heavily granulated promyelocytes. (bioportfolio.com)
  • The role of bone marrow morphology in the diagnosis of relapsed acute myeloid leukemia. (bioportfolio.com)
  • FES at 15q26.1 is also distal to the breakpoint in chromosome 15 in the translocation commonly seen in acute promyelocytic leukemia, t(15;17) (q24;q22). (nih.gov)
  • c-Abl is implicated in several human leukemias including 90-95% of chronic myelocytic leukemia, 20-25% of adult acute lymphoblastic leukemia and 2-5% of pediatric acute lymphoblastic leukemia. (scbt.com)
  • In chronic myelogenous leukemia and a subset of acute lymphoblastic leukemias, the c-Abl proto oncogene undergoes a (9;22) chromosomal translocation producing a novel rearranged chromosome (the Philadelphia chromosome) As the result of the fusion of c-Abl sequences from chromosome 9 to the Bcr gene on chromosome 22. (thermofisher.com)
  • And so, through this accidental discovery, one began to develop alkylating agents that turned out to be extremely successful in treating, and often curing, childhood leukemias, most notably acute lymphocytic leukemia, which turns out to be very sensitive to this and other related agents. (mit.edu)
  • BCR/ABL oncogenic tyrosine kinase transforms hematopoietic stem cells to induce chronic myelogenous leukemia (CML) and acute lymphocytic leukemia ( 1 ). (aacrjournals.org)
  • The majority of chronic myeloid leukemia (CML) and some of acute lymphocytic leukemia (ALL) cases are associated with possessing the BCR-Abl fusion protein from an oncogenic translocation, resulting in a constantly active form of Abl and rapid proliferation. (asu.edu)
  • Nucleotide sequence of Abelson murine leukemia virus genome: structural similarity of its transforming gene product to other onc gene products with tyrosine-specific kinase activity. (semanticscholar.org)
  • Multiple immunoglobulin heavy-chain gene transcripts in Abelson murine leukemia virus-transformed lymphoid cell lines. (semanticscholar.org)
  • Production of this factor is correlated with a tyrosine-specific protein kinase that is functionally active and is associated with the major Abelson MuLV gene product, P120. (semanticscholar.org)
  • Molecular biological analysis of three revertant clones isolated from the transformants showed that their morphological reversions were due to inactivation of the v-abl oncogene at multiple steps including transcription, translation or v-abl protein kinase activity itself without any change in structural gene expression of helper virus. (elsevier.com)
  • Collet, M. S. , and Erikson, R. L. , 1978, Protein kinase activity associated with the avian sarcoma virus Src gene product. (springer.com)
  • Adams J, Gerondakis S, Webb E, Corcoran LM, Cory S (1983) Cellular myc gene is altered by chromosome translocation to immunoglobulin locus in murine plasmacytomas and is rearranged similarly in human Burkitt lymphoma. (springer.com)
  • Barbacid M, Beemon K, Devare SG (1980a) Origin and functional properties of the major gene product of the Snyder-Theilen strain of feline sarcoma virus. (springer.com)
  • We have introduced a functionally rearranged murine ĸ light chain immunoglobulin (Ig) gene into an Abelson murine leukemia virus-transformed lymphoid cell line. (caltech.edu)
  • The virus encodes a single product, the v-Abl nonreceptor protein tyrosine kinase, which contains amino-terminal sequences derived from the Moloney leukemia virus gag gene fused to sequences from the c- abl protooncogene. (asm.org)
  • The human germ-line positions of the oncogenes ABL, SIS, and FES, the cellular counterparts of the v-onc genes of Abelson murine leukemia virus, simian sarcoma virus, and feline sarcoma virus, respectively, have been determined by in situ molecular hybridization of 3H-labeled v-onc gene probes to meiotic pachytene chromosomes. (nih.gov)
  • Adaptation of a retrovirus as a eucaryotic vector transmitting the herpes simplex virus thymidine kinase gene. (asm.org)
  • c-abl is the normal cellular homolog of the v-abl transforming gene of Abelson murine leukemia virus. (nih.gov)
  • We have constructed a panel of Abelson murine leukemia virus-transformed pre-B cells to study the organization of the mouse VH gene families. (rupress.org)
  • c-Abl is an oncogene, initially identified as the viral transforming gene of Abelson murine leukemia virus (A-MuLV). (scbt.com)
  • The c-abl gene, originally identified as the cellular homolog of the transforming gene of the Abelson murine leukemia virus, encodes a protein-tyrosine kinase of unknown function that is expressed in all mammalian tissues. (uptodate.com)
  • In 1973, Janet Rowley, unraveled the cytogenetic anatomy of the Philadelphia chromosome, which subsequently led to the identification of the BCR-ABL1 fusion gene and its principal pathogenetic role in the development of chronic myeloid leukemia. (haematologica.org)
  • The biology and treatment of patients with chronic myeloid leukemia (CML), a rare heterogeneous clonal hematopoietic stem cell disorder characterized by a consistent cytogenetic abnormality (the Philadelphia chromosome) and the presence of the BCR-ABL1 fusion gene, must surely be ranked as one of the most successful cancer medicine stories of the past century. (haematologica.org)
  • Abelson murine leukemia virus (A-MuLV) carries the gene v-abl, one of a group of oncogenes with structural and functional (tyrosine kinase) homology to three growth factor receptors. (edu.au)
  • BCR (Breakpoint Cluster Region) and ABL (Abelson Murine Leukemia Virus) is a fusion gene that causes cell division and inhibits apoptosis. (todaysclinician.com)
  • The role of oncogene expression in tumor metastasis was examined using the Abelson leukemia virus-transformed murine large cell lymphoma RAW117. (springer.com)
  • Dalla-Favera, R. , Wong-Staal, F. , and Gallo, R. C. , 1982, Oncogene amplication in promyelocytic leukemia cell line HL-60 and primary leukaemic cells of the same patient. (springer.com)
  • Expression of the protein tyrosine kinase encoded by the v- abl oncogene of Abelson murine leukemia virus (Ab-MLV) induces transformation of pre-B cells and immortalized fibroblast lines in vitro and causes pre-B-cell lymphoma in mice ( 37 ). (asm.org)
  • c-Abl (Abelson murine leukemia viral oncogene homolog 1, ABL1) is a 140 kDa proto-oncogene member of the Src family of non-receptor tyrosine kinases. (thermofisher.com)
  • The nonreceptor tyrosine kinase, encoded by the v-Abl oncogene of Abelson murine leukemia virus induces transformation of progenitor B cells. (elsevier.com)
  • A highly efficient helper virus commonly used when growing A-MuLV in vitro is Moloney murine leukemia virus (M-MuLV). (blogspot.com)
  • CTL directed against the Moloney murine leukemia virus (MuLV) epitope SSWDFITV recognize Moloney MuLV-induced tumor cells, but do not recognize cells transformed by the closely related Friend MuLV. (jimmunol.org)
  • His discovery of the tyrosine kinase activity in the ABL1 protein and the demonstration of the BCR-ABL oncoproteins in leukemias was one of the preclinical discoveries that led to the development of Gleevec, the first targeted therapy for chronic myelogenous leukemia. (wikipedia.org)
  • PURPOSE: This phase II trial is studying how well giving bryostatin 1 together with rituximab works in treating patients with B-cell non-Hodgkin's lymphoma or chronic lymphocytic leukemia that has not responded to previous treatment with rituximab. (bioportfolio.com)
  • Determine the feasibility and safety of bryostatin 1 and rituximab in patients with rituximab-refractory indolent B-cell non-Hodgkin's lymphoma or chronic lymphocytic leukemia (CLL). (bioportfolio.com)
  • PROJECTED ACCRUAL: Approximately 18-48 patients (9-24 with non-Hodgkin's lymphoma and 9-24 with chronic lymphocytic leukemia) will be accrued for this study within 12-30 months. (bioportfolio.com)
  • Updated results from the phase 3 HELIOS study of ibrutinib, bendamustine, and rituximab in relapsed chronic lymphocytic leukemia/small lymphocytic lymphoma. (bioportfolio.com)
  • We report follow-up results from the randomized, placebo-controlled, phase 3 HELIOS trial of ibrutinib+bendamustine and rituximab (BR) for previously treated chronic lymphocytic leukemia (CLL)/small l. (bioportfolio.com)
  • Dr. Goff's work led to realization that ABL is aberrantly expressed in chronic myelogenous leukemia in humans and led to the derivation of the efficacious anticancer drug Gleevec. (osu.edu)
  • The t(9;22) translocation results in the head-to-tail fusion of the BCR and c-Abl genes present in many cases of chronic myelogeneous leukemia. (thermofisher.com)
  • So having said that, I want to go to a different kind of leukemia, and this is called chronic myelogenous leukemia, to give you an indication of the path of discovery that led from its original description to the development of rather successful treatments. (mit.edu)
  • So, chronic myelogenous leukemia, I mentioned the prefix myelo- last time or the time before referring to bone marrow, and this is a leukemia of cells coming from the bone marrow from the myeloid cells in the bone marrow, which are the precursors of things like macrophages and granulocytes. (mit.edu)
  • So, these are cells which are playing an important role in the immune response, and during this chronic myelogenous leukemia disease, which is called CML, there could be a period of three or four years where individuals develop large numbers of these cells in their blood stream. (mit.edu)
  • BCR/ABL kinase-positive chronic myelogenous leukemia (CML) cells display genomic instability leading to point mutations in various genes including bcr/abl and p53 , eventually causing resistance to imatinib and malignant progression of the disease. (aacrjournals.org)
  • Chronic myelogenous leukemia (CML) is a human disease associated with a consistent chromosomal translocation that results in sequences from the c-abl locus on chromosome 9 being fused to sequences in a breakpoint cluster region (bcr) on chromosome 22. (sciencemag.org)
  • With the deaths of Janet Rowley and John Goldman in December 2013, the world lost two pioneers in the field of chronic myeloid leukemia. (haematologica.org)
  • Then, in collaboration with Brian Druker, he led efforts to develop ABL1 tyrosine kinase inhibitors for the treatment of patients with chronic myeloid leukemia in the late 1990s. (haematologica.org)
  • This tribute provides an outline of the remarkable story of chronic myeloid leukemia, and in writing it, it is clear that the historical triumph of biomedical science over this leukemia cannot be considered without appreciating the work of both Janet Rowley and John Goldman. (haematologica.org)
  • Survival with chronic myeloid leukemia over time (1993-2013): the German CML-Study Group experience. (haematologica.org)
  • Imatinib sensitivity in BCR-ABL1-positive chronic myeloid leukemia cells is regulated by the remaining normal ABL1 allele. (mousemine.org)
  • Intolerance to tyrosine kinase inhibitors in chronic myeloid leukemia: Definitions and clinical implications. (mysciencework.com)
  • Tyrosine kinase inhibitor (TKI) treatment targeting breakpoint cluster region-Abelson murine leukemia virus, the cause of chronic myeloid leukemia (CML), has revolutionized therapy for patients with this disease. (mysciencework.com)
  • Another example of balanced translocation is the Philadelphia chromosome seen in chronic myeloid leukemia. (todaysclinician.com)
  • Abelson murine leukemia virus-induced tumors elicit antibodies against a host cell protein, P50. (semanticscholar.org)
  • An alteration of the human c-abl protein in K562 leukemia cells unmasks associated tyrosine kinase activity" (PDF). (wikipedia.org)
  • Furthermore, the serum from recovered mice (Abelson tumor serum) has been used as a source of antibodies to abl antigens present in the M r 120, 000 A-MuLV-encoded gag-abl fusion protein (p120). (elsevier.com)
  • Nevertheless, an M r 95,000 protein was identified on the L1-2 cell surface using the Abelson tumor serum as a probe. (elsevier.com)
  • Abl Protein Tyrosine Kinase (AbI) is a truncated form of the v-AbI Protein Tyrosine Kinase, a partner in the Gag-Abl fusion protein of the Abelson murine leukemia virus. (neb.com)
  • Isolated from a strain of E. coli that carries the truncated AbI Protein Kinase encoded by the Abelson murine leukemia virus under the control of a T7 expression system (kindly provided by Dr. S. Goff). (neb.com)
  • Abrams HD, Rohrschneider LR, Eisenman RN (1982) Nuclear location of the putative transforming protein of avian myelocytomatosis virus. (springer.com)
  • Barbacid M, Breitman ML, Lauver AV, Long LK, Vogt PK (1981) The transformation-specific protein of avian (Fujinami and PRC-II) and feline (Snyder-Theilen and Gardner-Amstein) sarcoma viruses are immunologically related. (springer.com)
  • Abelson murine leukemia virus (Ab-MLV) encodes the v-Abl protein tyrosine kinase and induces transformation of immortalized fibroblast lines and pre-B cells. (asm.org)
  • Half-life of the Rous sarcoma virus transforming protein pp60src and its associated kinase activity. (asm.org)
  • By constructing recombinants between c- and v-abl retroviruses, we show that a point mutation in c-Abl is sufficient to change the myristoylated form of c-Abl into a protein able to transform fibroblasts, but not capable of transforming bone marrow or inducing Abelson disease. (nih.gov)
  • Dessa proteiner visa en mångfald av cellulära funktioner inklusive kärnkraft import / export av RNA och protein, transkriptionell reglering, och apoptos. (jove.com)
  • Här presenterar vi en metod för analys av protein människohandel och skytteltrafik aktivitet mellan primär och transformerade däggdjursceller. (jove.com)
  • Efter uttryck, är de celler som smält med polyetylenglykol och protein lokaliseringar kan sedan avbildas med en rad olika metoder. (jove.com)
  • By analogy to the gag/abl fusion protein of Abelson murine leukemia virus, the replacement of amino terminal c-abl sequences by bcr sequences in P210 may create a transforming protein involved in CML. (sciencemag.org)
  • The phosphorylation of the Fujinami sarcoma virus transforming protein (FSV P140gag-fps) is complex, reversible and affects its tyrosine specific protein kinase activity and transforming function. (ubc.ca)
  • Linzer and Levine (Linzer and Levine 1979) found that the 54-kDa protein was overexpressed in a wide variety of murine SV40 transformed cells, but also in uninfected embryonic carcinoma cells. (p53.fr)
  • Anderson SM, Hayward WS, Neel BG, Hanafusa H (1980) Avian erythroblastosis virus produces two mRNA's. (springer.com)
  • Tooze J (1980) DNA tumor viruses. (p53.fr)
  • Rat embryo fibroblasts transformed by Abelson murine leukemia virus (MuLV) produce and release a transforming growth factor (TGF). (semanticscholar.org)
  • Lochhead RB, Sonderegger FL, Ma Y, Brewster JE, Cornwall D, Maylor-Hagen H, Miller JC, Zachary JF, Weis JH, Weis JJ: Endothelial cells and fibroblasts amplify the arthritogenic type I IFN response in murine Lyme disease and are major sources of chemokines in Borrelia burgdorferi-infected joint tissue. (exbio.cz)
  • Transformation induced by Abelson murine leukemia virus involves production of a polypeptide growth factor. (semanticscholar.org)
  • The Abelson murine leukemia virus (Ab-MLV, A-MuLV) is a retrovirus (Class VI) used to induce transformation of murine lymphoid cells. (blogspot.com)
  • Identifying substrates that interact with SH2 domains and probing the way these interactions contribute to transformation provide one approach to understanding how SH2-mediated signaling specificity contributes to host-virus interaction. (asm.org)
  • Proteins encoded by the INK4a/ARF locus also play a role in Abelson virus transformation. (novusbio.com)
  • Eftersom många proteiner visa förändrade lokalisering under omvandling eller som en följd av transformation, metoder för att effektivt karaktärisera dessa proteiner och de vägar som de deltar står för att förbättra förståelsen för onkogenes och öppna nya områden för läkemedelsutveckling inriktning. (jove.com)
  • Abelson virus transformation of an interleukin 2-dependent antigen-specific T-cell line. (edu.au)
  • Deregulation of p27, Foxo3a, c-myc, and NF-κB/Rel was found to be associated with the malignant transformation of SCID temperature-sensitive form of Abelson murine leukemia virus pre-B cells. (elsevier.com)
  • Corrective recombination of mouse immunoglobulin kappa alleles in Abelson murine leukemia virus-transformed pre-B cells. (asm.org)
  • A chromosomal segment with a unique structure around the immunoglobulin heavy chain joining region (JH) has been molecularly cloned from an Abelson murine leukemia virus-transformed cell line. (pnas.org)
  • Adams JM, Gerondakis S, Webb E, Mitchell J, Bernard O, Cory S (1982) Transcriptionally active DNA region that rearranges frequently in murine lymphoid tumors. (springer.com)
  • Antisera reactive with the Abelson murine leukemia virus (A-MuLV)-specified P120 (anti-AbT sera) were produced in C57L/J mice. (caltech.edu)
  • A-MuLV causes a rapidly progressive lymphosarcoma known as Abelson disease in mice, which is a type of leukemia that does not involve the thymus. (blogspot.com)
  • The Abelson murine leukemia virus (A-MuLV)-transformed lymphoid cell line L1-2 is exceptional since it is rejected following transplantation into syngeneic C57L mice. (elsevier.com)
  • Der, C. J., Krontiris, T. G. , and Cooper, G. M. , 1982, Transforming genes of human bladder and lung carcinoma cell lines are homologous to the ras genes of Harvey and Kisten sarcoma viruses. (springer.com)
  • Andersen PR, Devare SG, Tronick SR, Ellis RW, Aaronson SA, Scolnick EM (1981) Generation of BALB/MuSV and Ha-MuSV by type C virus transduction of homologous transforming genes from different species. (springer.com)
  • Discusi n: Es importante tener en cuenta a este tipo de tumores, dado que los s ntomas, signos cl nicos y los estudios de im genes son inespec ficos. (bireme.br)
  • Examples of point mutations are seen in RAS genes (Rat Sarcoma Virus genes) seen in pancreatic cancer and colorectal cancer and the BRAF mutation seen in malignant melanoma and papillary carcinoma of thyroid. (todaysclinician.com)
  • In Weinberg's lab, Tabin constructed murine leukemia virus, the first recombinant retrovirus that could be used as a eukaryotic vector. (wikipedia.org)
  • Abelson murine leukemia virus (Ab-MLV) is a replication-defective retrovirus that transforms pre-B cells and NIH 3T3 cells in vitro and induces a pre-B cell lymphoma in vivo (reviewed in reference 40 ). (asm.org)
  • Abelson murine leukemia virus - a retrovirus belonging to the type C retrovirus group subfamily (family Oncovirinae), which is associated with leukemia. (thefreedictionary.com)
  • Human T-lymphotropic virus (HTLV) is a human, single-stranded RNA retrovirus that causes T-cell leukemia and T-cell lymphoma in adults and may also be involved in certain demyelinating diseases, including tropical spastic paraparesis . (bionity.com)
  • Abelson HT, Rabstein LS (1970) Lymphosarcoma: Virus-induced thymic-independent disease in mice. (springer.com)
  • Dr. Goff developed the first methods to deliver recombinant DNA molecules into mammalian cells by employing SV40 virus, and was among the first to prepare molecular clones of retroviruses. (osu.edu)
  • Levine AJ (1988) Oncogenes of DNA tumor viruses. (p53.fr)
  • Transformed Fisher rat fibroblast cell lines by Abelson murine leukemia virus frequently revert to the normal phenotype in usual culture conditions. (elsevier.com)
  • Machida, CA & Kabat, D 1983, ' Relationship of Surface Membranes of Lymphoid Cells Transformed by Abelson Murine Leukemia Virus to Tumor Rejection ', Cancer Research , vol. 43, no. 3, pp. 1275-1281. (elsevier.com)
  • This study shows that induction of tumor-specific CD4 + T cells by vaccination with a specific viral T helper epitope, contained within a synthetic peptide, results in protective immunity against major histocompatibility complex (MHC) class II negative, virus-induced tumor cells. (rupress.org)
  • For example, some of the most effective chemotherapeutics against childhood leukemia are alkylating agents, which attach methyl and ethyl groups to target molecules inside cells. (mit.edu)
  • And keep in mind that when we talk about leukemia, the suffix -emia refers to blood generally, and leuk- once again refers to white blood, i.e. an excess of white blood cells in the blood. (mit.edu)
  • CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells. (exbio.cz)
  • Watanabe Y, Akaike T: Activation signal induces the expression of B cell-specific CD45R epitope (6B2) on murine T cells. (exbio.cz)
  • Impaired MMR activity in leukemia cells was associated with better survival, accumulation of p53 but not of p73, and lack of activation of caspase 3 after MNNG treatment. (aacrjournals.org)
  • Our recent findings indicated that BCR/ABL-positive leukemia cells accumulate more DNA lesions due to enhanced levels of endogenous reactive oxygen species, resulting in point mutations ( 5 , 6 ). (aacrjournals.org)
  • The virus activates a subset of T-helper cells called Th1 cells. (bionity.com)
  • We have previously described monoclonal antibodies (MAbs) to histone 2B (H2B) on the cell surface of H. capsulatum yeast cells that modify the course of murine histoplasmosis ( 42 ). (asm.org)
  • bone marrow disorder known as T-cell prolymphocytic leukemia (T-cell PLL) which does not tend to respond well to conventional treatment with chemotherapy. (bioportfolio.com)
  • A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) producing leukemia of the reticulum-cell type with massive infiltration of liver, spleen, and bone marrow. (bioportfolio.com)
  • Expanded HIV-1 cellular tropism by phenotypic mixing with murine endogenous retroviruses. (semanticscholar.org)
  • However, this is only possible when the host cell is co-infected with a helper virus which provides functions it needs to be able to replicate which is does not code for in its own genome such as a reverse transcriptase and some major structural proteins. (blogspot.com)
  • Witte's research has contributed to the understanding of human leukemias, immune disorders and stem cell activity in cancers of the epithelium. (wikipedia.org)
  • Single dose versus multiple doses of rituximab for preemptive therapy of Epstein-Barr virus reactivation after hematopoietic cell transplantation. (bioportfolio.com)
  • Epstein-Barr virus (EBV) reactivation is an unresolved medical issue after allogeneic hematopoietic stem cell transplantation (HSCT). (bioportfolio.com)
  • c-Abl Inhibitors offered by Santa Cruz inhibit c-Abl and, in some cases, other cell signaling and leukemia related proteins. (scbt.com)
  • 1. A monoclonal antibody of the class IgG or IgM, derived from the fusion of a murine myeloma cell and a murine antibody-producing lymphoid cell, demonstrating specific reactivity to a phosphotyrosine moiety on phosphotyrosine-containing proteins. (google.ca)
  • 7. A murine hybridoma cell line characterized by its production of monoclonal antibodies of the class IgG or IgM demonstrating specific reactivity to a a phosphotyrosine moiety on phosphotyrosine-containing proteins. (google.ca)
  • Publications] Toshihiro ITO: 'Monoclonal antibody against murine T cell receptor Vα14 cross-reacts with human CD3-ε and detects disulfied-linked dimeric form' Intl.Immunol.3. (nii.ac.jp)
  • HTLV-I is an abbreviation for the human T-cell lymphotropic virus type 1 , also called the Adult T-cell lymphoma virus type 1 , a virus that has been seriously implicated in several kinds of diseases including HTLV-I-associated myelopathy , Strongyloides stercoralis hyper-infection, and a virus cancer link for leukemia (see adult T-cell leukemia/lymphoma ). (bionity.com)
  • WB: A431 cell lysate, PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate (treated and untreated), RAW264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate (treated and untreated). (abcam.cn)
  • Monoclonal antibodies (MAbs) to a cell surface histone on Histoplasma capsulatum modify murine infection and decrease the growth of H. capsulatum within macrophages. (asm.org)
  • The Abelson murine leukemia virus is named for the American pediatrician Herbert T. Abelson who first described and isolated it. (blogspot.com)
  • A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) arising during the propagation of S37 mouse sarcoma, and causing lymphoid leukemia in mice. (bioportfolio.com)
  • Barbacid M, Lauver AV, Devare SG (1980b) Biochemical and immunological characterization of polyproteins encoded for by the McDonough, Gardner-Amstein, and Snyder-Theilen strains of feline sarcoma virus. (springer.com)
  • These data suggest that alteration of the FLVRES pocket affects the ability of v-Abl to interact with at least some of its substrates in a temperature-dependent fashion and identify a novel type of temperature-sensitive Abelson virus. (asm.org)
  • The temperature-sensitive form of Abelson murine leukemia virus offers a reversible model to study the role of v-Abl in regulating growth and differentiation. (elsevier.com)
  • This finding influenced the development of targeted drugs like Ibrutinib to treat leukemia and lymphoma. (wikipedia.org)
  • Rapid virus generation, broad host range, efficient RNA replication in the cytoplasm, and high expression levels are features that have made the use of Semliki Forest virus (SFV) vectors attractive. (gtmb.org)
  • Thymic targets for Abelson murine leukemia virus are early gamma/delta T lymphocytes. (linkedlifedata.com)
  • Thus, a potential CTL epitope present in the Friend virus sequence is not properly processed and presented because of a natural flanking aspartic acid that obliterates the correct C-terminal cleavage site. (jimmunol.org)
  • Normal ABL1 is a tumor suppressor and therapeutic target in human and mouse leukemias expressing oncogenic ABL1 kinases. (mousemine.org)
  • Adult T-lymphotropic virus (ATLV) is a strain of this disease that affects primarily adults. (bionity.com)