Abelson murine leukemia virus: A replication-defective strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) capable of transforming lymphoid cells and producing a rapidly progressing lymphoid leukemia after superinfection with FRIEND MURINE LEUKEMIA VIRUS; MOLONEY MURINE LEUKEMIA VIRUS; or RAUSCHER VIRUS.Leukemia Virus, Murine: Species of GAMMARETROVIRUS, containing many well-defined strains, producing leukemia in mice. Disease is commonly induced by injecting filtrates of propagable tumors into newborn mice.Oncogene Proteins v-abl: Transforming proteins encoded by the abl oncogenes. Oncogenic transformation of c-abl to v-abl occurs by insertional activation that results in deletions of specific N-terminal amino acids.Moloney murine leukemia virus: A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) arising during the propagation of S37 mouse sarcoma, and causing lymphoid leukemia in mice. It also infects rats and newborn hamsters. It is apparently transmitted to embryos in utero and to newborns through mother's milk.Cell Transformation, Viral: An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.Leukemia, Experimental: Leukemia induced experimentally in animals by exposure to leukemogenic agents, such as VIRUSES; RADIATION; or by TRANSPLANTATION of leukemic tissues.AKR murine leukemia virus: A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) isolated from spontaneous leukemia in AKR strain mice.Friend murine leukemia virus: A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) producing leukemia of the reticulum-cell type with massive infiltration of liver, spleen, and bone marrow. It infects DBA/2 and Swiss mice.Genes, abl: Retrovirus-associated DNA sequences (abl) originally isolated from the Abelson murine leukemia virus (Ab-MuLV). The proto-oncogene abl (c-abl) codes for a protein that is a member of the tyrosine kinase family. The human c-abl gene is located at 9q34.1 on the long arm of chromosome 9. It is activated by translocation to bcr on chromosome 22 in chronic myelogenous leukemia.Thymoma: A neoplasm originating from thymic tissue, usually benign, and frequently encapsulated. Although it is occasionally invasive, metastases are extremely rare. It consists of any type of thymic epithelial cell as well as lymphocytes that are usually abundant. Malignant lymphomas that involve the thymus, e.g., lymphosarcoma, Hodgkin's disease (previously termed granulomatous thymoma), should not be regarded as thymoma. (From Stedman, 25th ed)Cell Transformation, Neoplastic: Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.Helper Viruses: Viruses which enable defective viruses to replicate or to form a protein coat by complementing the missing gene function of the defective (satellite) virus. Helper and satellite may be of the same or different genus.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Oncogenes: Genes whose gain-of-function alterations lead to NEOPLASTIC CELL TRANSFORMATION. They include, for example, genes for activators or stimulators of CELL PROLIFERATION such as growth factors, growth factor receptors, protein kinases, signal transducers, nuclear phosphoproteins, and transcription factors. A prefix of "v-" before oncogene symbols indicates oncogenes captured and transmitted by RETROVIRUSES; the prefix "c-" before the gene symbol of an oncogene indicates it is the cellular homolog (PROTO-ONCOGENES) of a v-oncogene.Genes, Viral: The functional hereditary units of VIRUSES.Leukemia Virus, Feline: A species of GAMMARETROVIRUS causing leukemia, lymphosarcoma, immune deficiency, or other degenerative diseases in cats. Several cellular oncogenes confer on FeLV the ability to induce sarcomas (see also SARCOMA VIRUSES, FELINE).Viral Proteins: Proteins found in any species of virus.Sarcoma Viruses, Feline: Species of GAMMARETROVIRUS isolated from fibrosarcoma in cats. The viruses are actually recombinant feline leukemia viruses (FeLV) where part of the genome has been replaced by cellular oncogenes. It is unique to individuals and not transmitted naturally to other cats. FeSVs are replication defective and require FeLV to reproduce.Rauscher Virus: A strain of MURINE LEUKEMIA VIRUS associated with mouse tumors similar to those caused by the FRIEND MURINE LEUKEMIA VIRUS. It is a replication-competent murine leukemia virus. It can act as a helper virus when complexing with a defective transforming component, RAUSCHER SPLEEN FOCUS-FORMING VIRUS.B-Lymphocytes: Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.Mice, Inbred Strains: Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.Retroviridae Infections: Virus diseases caused by the RETROVIRIDAE.Leukemia Virus, Bovine: The type species of DELTARETROVIRUS that causes a form of bovine lymphosarcoma (ENZOOTIC BOVINE LEUKOSIS) or persistent lymphocytosis.Cell Line, Transformed: Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.DNA, Viral: Deoxyribonucleic acid that makes up the genetic material of viruses.Mice, Inbred BALB CProtein-Tyrosine Kinases: Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors.Leukemia: A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)Defective Viruses: Viruses which lack a complete genome so that they cannot completely replicate or cannot form a protein coat. Some are host-dependent defectives, meaning they can replicate only in cell systems which provide the particular genetic function which they lack. Others, called SATELLITE VIRUSES, are able to replicate only when their genetic defect is complemented by a helper virus.Retroviridae: Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).Mice, Inbred AKRLymphocytes: White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Virus Replication: The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.Clone Cells: A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)Protein Kinases: A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.Lymphoma: A general term for various neoplastic diseases of the lymphoid tissue.Mink Cell Focus-Inducing Viruses: Strains of MURINE LEUKEMIA VIRUS discovered in 1976 by Hartley, Wolford, Old, and Rowe and so named because the viruses originally isolated had the capacity to transform cell foci in mink cell cultures. MCF viruses are generated by recombination with ecotropic murine leukemia viruses including AKR, Friend, Moloney, and Rauscher, causing ERYTHROLEUKEMIA and severe anemia in mice.RNA-Directed DNA Polymerase: An enzyme that synthesizes DNA on an RNA template. It is encoded by the pol gene of retroviruses and by certain retrovirus-like elements. EC 2.7.7.49.Receptors, Virus: Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Proviruses: Duplex DNA sequences in eukaryotic chromosomes, corresponding to the genome of a virus, that are transmitted from one cell generation to the next without causing lysis of the host. Proviruses are often associated with neoplastic cell transformation and are key features of retrovirus biology.Gene Products, gag: Proteins coded by the retroviral gag gene. The products are usually synthesized as protein precursors or POLYPROTEINS, which are then cleaved by viral proteases to yield the final products. Many of the final products are associated with the nucleoprotein core of the virion. gag is short for group-specific antigen.Tumor Virus Infections: Infections produced by oncogenic viruses. The infections caused by DNA viruses are less numerous but more diverse than those caused by the RNA oncogenic viruses.Gammaretrovirus: A genus of RETROVIRIDAE comprising endogenous sequences in mammals, related RETICULOENDOTHELIOSIS VIRUSES, AVIAN, and a reptilian virus. Many species contain oncogenes and cause leukemias and sarcomas.Leukemia, Myeloid, Acute: Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.Virus Integration: Insertion of viral DNA into host-cell DNA. This includes integration of phage DNA into bacterial DNA; (LYSOGENY); to form a PROPHAGE or integration of retroviral DNA into cellular DNA to form a PROVIRUS.Leukemia Virus, Gibbon Ape: A species of GAMMARETROVIRUS causing leukemia in the gibbon ape. Natural transmission is by contact.RNA, Viral: Ribonucleic acid that makes up the genetic material of viruses.3T3 Cells: Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Viral Envelope Proteins: Layers of protein which surround the capsid in animal viruses with tubular nucleocapsids. The envelope consists of an inner layer of lipids and virus specified proteins also called membrane or matrix proteins. The outer layer consists of one or more types of morphological subunits called peplomers which project from the viral envelope; this layer always consists of glycoproteins.Mink: Carnivores of genus Mustela of the family MUSTELIDAE. The European mink, which has white upper and lower lips, was widely trapped for commercial purposes and is classified as endangered. The American mink, lacking a white upper lip, is farmed commercially.Immunoglobulin mu-Chains: The class of heavy chains found in IMMUNOGLOBULIN M. They have a molecular weight of approximately 72 kDa and they contain about 57 amino acid residues arranged in five domains and have more oligosaccharide branches and a higher carbohydrate content than the heavy chains of IMMUNOGLOBULIN G.Blotting, Southern: A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.DNA Restriction Enzymes: Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.Preleukemia: Conditions in which the abnormalities in the peripheral blood or bone marrow represent the early manifestations of acute leukemia, but in which the changes are not of sufficient magnitude or specificity to permit a diagnosis of acute leukemia by the usual clinical criteria.Human T-lymphotropic virus 1: A strain of PRIMATE T-LYMPHOTROPIC VIRUS 1 isolated from mature T4 cells in patients with T-lymphoproliferation malignancies. It causes adult T-cell leukemia (LEUKEMIA-LYMPHOMA, T-CELL, ACUTE, HTLV-I-ASSOCIATED), T-cell lymphoma (LYMPHOMA, T-CELL), and is involved in mycosis fungoides, SEZARY SYNDROME and tropical spastic paraparesis (PARAPARESIS, TROPICAL SPASTIC).Gene Rearrangement, B-Lymphocyte, Heavy Chain: Ordered rearrangement of B-lymphocyte variable gene regions of the IMMUNOGLOBULIN HEAVY CHAINS, thereby contributing to antibody diversity. It occurs during the first stage of differentiation of the IMMATURE B-LYMPHOCYTES.Plasmacytoma: Any discrete, presumably solitary, mass of neoplastic PLASMA CELLS either in BONE MARROW or various extramedullary sites.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Gene Products, env: Retroviral proteins, often glycosylated, coded by the envelope (env) gene. They are usually synthesized as protein precursors (POLYPROTEINS) and later cleaved into the final viral envelope glycoproteins by a viral protease.Retroviridae Proteins, Oncogenic: Retroviral proteins that have the ability to transform cells. They can induce sarcomas, leukemias, lymphomas, and mammary carcinomas. Not all retroviral proteins are oncogenic.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Repetitive Sequences, Nucleic Acid: Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Sarcoma Viruses, Murine: A group of replication-defective viruses, in the genus GAMMARETROVIRUS, which are capable of transforming cells, but which replicate and produce tumors only in the presence of Murine leukemia viruses (LEUKEMIA VIRUS, MURINE).Genes: A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Virion: The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.
(1/205) p53 mediates apoptotic crisis in primary Abelson virus-transformed pre-B cells.

Transformation of pre-B cells by Abelson murine leukemia virus (Ab-MLV) involves a balance between positive, growth-stimulatory signals from the v-Abl oncoprotein and negative regulatory cues from cellular genes. This phenomenon is reflected by the clonal selection that occurs during Ab-MLV-mediated transformation in vivo and in vitro. About 50% of all Ab-MLV-transformed pre-B cells express mutant forms of p53 as they emerge from this process, suggesting that this protein may play an important role in the transformation process. Consistent with this idea, expression of p19(Arf), a protein whose function depends on the presence of a functional p53, is required for the apoptotic crisis that characterizes primary Ab-MLV transformants. To test the role of p53 in pre-B-cell transformation directly, we examined the response of Trp53(-/-) mice to Ab-MLV. The absence of p53 shortens the latency of Abelson disease induction but does not affect the frequency of cells susceptible to Ab-MLV-induced transformation. However, primary transformants derived from the null animals bypass the apoptotic crisis that characterizes the transition from primary transformant to fully malignant cell line. These effects do not require p21(Cip-1), a major downstream target of p53; however, consistent with a role of p19(Arf), transformants expressing mutant p53 and abundant p19 retain wild-type p19 sequences.  (+info)

(2/205) Functional characterization of B lymphocytes generated in vitro from embryonic stem cells.

To study molecular events involved in B lymphocyte development and V(D)J rearrangement, we have established an efficient system for the differentiation of embryonic stem (ES) cells into mature Ig-secreting B lymphocytes. Here, we show that B lineage cells generated in vitro from ES cells are functionally analogous to normal fetal liver-derived or bone marrow-derived B lineage cells at three important developmental stages: first, they respond to Flt-3 ligand during an early lymphopoietic progenitor stage; second, they become targets for Abelson murine leukemia virus (A-MuLV) infection at a pre-B cell stage; third, they secrete Ig upon stimulation with lipopolysaccharide at a mature mitogen-responsive stage. Moreover, the ES cell-derived A-MuLV-transformed pre-B (EAB) cells are phenotypically and functionally indistinguishable from standard A-MuLV-transformed pre-B cells derived from infection of mouse fetal liver or bone marrow. Notably, EAB cells possess functional V(D)J recombinase activity. In particular, the generation of A-MuLV transformants from ES cells will provide an advantageous system to investigate genetic modifications that will help to elucidate molecular mechanisms in V(D)J recombination and in A-MuLV-mediated transformation.  (+info)

(3/205) Drosophila abelson interacting protein (dAbi) is a positive regulator of abelson tyrosine kinase activity.

Human and mouse Abelson interacting proteins (Abi) are SH3-domain containing proteins that bind to the proline-rich motifs of the Abelson protein tyrosine kinase. We report a new member of this gene family, a Drosophila Abi (dAbi) that is a substrate for Abl kinase and that co-immunoprecipitates with Abl if the Abi SH3 domain is intact. We have identified a new function for both dAbi and human Abi-2 (hAbi-2). Both proteins activate the kinase activity of Abl as assayed by phosphorylation of the Drosophila Enabled (Ena) protein. Removal of the dAbi SH3 domain eliminates dAbi's activation of Abl kinase activity. dAbi is an unstable protein in cells and is present at low steady state levels but its protein level is increased coincident with phosphorylation by Abl kinase. Expression of the antisense strand of dAbi reduces dAbi protein levels and abolishes activation of Abl kinase activity. Modulation of Abi protein levels may be an important mechanism for regulating the level of Abl kinase activity in the cell.  (+info)

(4/205) Inhibition of v-Abl transformation by p53 and p19ARF.

Tumorigenesis is a multistep process that involves the activation of oncogenes and the inactivation of tumor suppressor genes. The transforming activity of the v-Abl oncogene of Abelson murine leukemia virus (A-MuLV) in immortal cell lines has been well studied, while the effects of v-Abl in primary fibroblasts are less clear. Here we show that v-Abl causes cell cycle arrest in primary mouse embryonic fibroblasts (MEFs) and elevated levels of both p53 and the cyclin-dependent kinase inhibitor p21Cip. p53-/- or p19ARF-/- MEFs were resistant to v-Abl-induced cell cycle arrest. Although wild-type MEFs were resistant to v-Abl transforming activity, p53-/- or p19ARF-/- MEFs were susceptible. The results indicate that loss of p19ARF and p53 function plays an important role during the transformation of primary cells by v-Abl. We suggest that although v-Abl is a potent oncogene, its full potential transforming activity cannot be realized until the ARF-, and p53-dependent growth inhibitory pathway is disabled. We also show that p53 is not the mediator of v-Abl toxicity in immortal fibroblasts and does not determine the susceptibility of immortal fibroblasts to v-Abl transformation.  (+info)

(5/205) The carboxyl terminus of v-Abl protein can augment SH2 domain function.

Abelson murine leukemia virus (Ab-MLV) transforms NIH 3T3 and pre-B cells via expression of the v-Abl tyrosine kinase. Although the enzymatic activity of this molecule is absolutely required for transformation, other regions of the protein are also important for this response. Among these are the SH2 domain, involved in phosphotyrosine-dependent protein-protein interactions, and the long carboxyl terminus, which plays an important role in transformation of hematopoietic cells. Important signals are sent from each of these regions, and transformation is most likely orchestrated by the concerted action of these different parts of the protein. To explore this idea, we compared the ability of the v-Src SH2 domain to substitute for that of v-Abl in the full-length P120 v-Abl protein and in P70 v-Abl, a protein that lacks the carboxyl terminus characteristic of Abl family members. Ab-MLV strains expressing P70/S2 failed to transform NIH 3T3 cells and demonstrated a greatly reduced capacity to mediate signaling events associated with the Ras-dependent mitogen-activated protein (MAP) kinase pathway. In contrast, Ab-MLV strains expressing P120/S2 were indistinguishable from P120 with respect to these features. Analyses of additional mutants demonstrated that the last 162 amino acids of the carboxyl terminus were sufficient to restore transformation. These data demonstrate that an SH2 domain with v-Abl substrate specificity is required for NIH 3T3 transformation in the absence of the carboxyl terminus and suggest that cooperativity between the extreme carboxyl terminus and the SH2 domain facilitates the transmission of transforming signals via the MAP kinase pathway.  (+info)

(6/205) Transgenic human lambda 5 rescues the murine lambda 5 nullizygous phenotype.

The human lambda 5 (hu lambda 5) gene is the structural homologue of the murine lambda 5 (m lambda 5) gene and is transcriptionally active in pro-B and pre-B lymphocytes. The lambda 5 and VpreB polypeptides together with the Ig mu H chain and the signal-transducing subunits, Ig alpha and Ig beta, comprise the pre-B cell receptor. To further investigate the pro-B/pre-B-specific transcription regulation of hu lambda 5 in an in vivo model, we generated mouse lines that contain a 28-kb genomic fragment encompassing the entire hu lambda 5 gene. High levels of expression of the transgenic hu lambda 5 gene were detected in bone marrow pro-B and pre-B cells at the mRNA and protein levels, suggesting that the 28-kb transgene fragment contains all the transcriptional elements necessary for the stage-specific B progenitor expression of hu lambda 5. Flow cytometric and immunoprecipitation analyses of bone marrow cells and Abelson murine leukemia virus-transformed pre-B cell lines revealed the hu lambda 5 polypeptide on the cell surface and in association with mouse Ig mu and mouse VpreB. Finally, we found that the hu lambda 5 transgene is able to rescue the pre-B lymphocyte block when bred onto the m lambda 5-/- background. Therefore, we conclude that the hu lambda 5 polypeptide can biochemically and functionally substitute for m lambda 5 in vivo in pre-B lymphocyte differentiation and proliferation. These studies on the mouse and human pre-B cell receptor provide a model system to investigate some of the molecular requirements necessary for B cell development.  (+info)

(7/205) Activation of V(D)J recombination induces the formation of interlocus joints and hybrid joints in scid pre-B-cell lines.

V(D)J recombination is the mechanism by which antigen receptor genes are assembled. The site-specific cleavage mediated by RAG1 and RAG2 proteins generates two types of double-strand DNA breaks: blunt signal ends and covalently sealed hairpin coding ends. Although these DNA breaks are mainly resolved into coding joints and signal joints, they can participate in a nonstandard joining process, forming hybrid and open/shut joints that link coding ends to signal ends. In addition, the broken DNA molecules excised from different receptor gene loci could potentially be joined to generate interlocus joints. The interlocus recombination process may contribute to the translocation between antigen receptor genes and oncogenes, leading to malignant transformation of lymphocytes. To investigate the underlying mechanisms of these nonstandard recombination events, we took advantage of recombination-inducible cell lines derived from scid homozygous (s/s) and scid heterozygous (s/+) mice by transforming B-cell precursors with a temperature-sensitive Abelson murine leukemia virus mutant (ts-Ab-MLV). We can manipulate the level of recombination cleavage and end resolution by altering the cell culture temperature. By analyzing various recombination products in scid and s/+ ts-Ab-MLV transformants, we report in this study that scid cells make higher levels of interlocus and hybrid joints than their normal counterparts. These joints arise concurrently with the formation of intralocus joints, as well as with the appearance of opened coding ends. The junctions of these joining products exhibit excessive nucleotide deletions, a characteristic of scid coding joints. These data suggest that an inability of scid cells to promptly resolve their recombination ends exposes the ends to a random joining process, which can conceivably lead to chromosomal translocations.  (+info)

(8/205) Loss of heterozygosity at the Ink4a/Arf locus facilitates Abelson virus transformation of pre-B cells.

In many tumor systems, analysis of cells for loss of heterozygosity (LOH) has helped to clarify the role of tumor suppressor genes in oncogenesis. Two important tumor suppressor genes, p53 and the Ink4a/Arf locus, play central roles in the multistep process of Abelson murine leukemia virus (Ab-MLV) transformation. p53 and the p53 regulatory protein, p19Arf, are required for the apoptotic crisis that characterizes the progression of primary transformed pre-B cells to fully malignant cell lines. To search for other tumor suppressor genes which may be involved in the Ab-MLV transformation process, we used endogenous proviral markers and simple-sequence length polymorphism analysis to screen Abelson virus-transformed pre-B cells for evidence of LOH. Our survey reinforces the role of the p53-p19 regulatory pathway in transformation; 6 of 58 cell lines tested had lost sequences on mouse chromosome 4, including the Ink4a/Arf locus. Consistent with this pattern, a high frequency of primary pre-B-cell transformants derived from Ink4a/Arf +/- mice became established cell lines. In addition, half of them retained the single copy of the locus when the transformation process was complete. These data demonstrate that a single copy of the Ink4a/Arf locus is not sufficient to fully mediate the effects of these genes on transformation.  (+info)

*  Abelson murine leukemia virus
The Abelson murine leukemia virus (Ab-MLV or A-MuLV) is a retrovirus (Class VI) used to induce transformation of murine ... The Abelson murine leukemia virus is named for the American pediatrician Herbert T. Abelson, who first described and isolated ... Shields A, Rosenberg N, Baltimore D (1979). "Virus production by Abelson murine leukemia virus-transformed lymphoid cells". J. ... Abelson murine leukemia virus at the US National Library of Medicine Medical Subject Headings (MeSH). ...
*  Owen Witte
"President's Cancer Panel". Witte ON, Dasgupta A, Baltimore D (February 28, 1980). "Abelson murine leukemia virus protein is ... Konopka JB, Watanabe SM, Witte ON (July 1984). "An alteration of the human c-abl protein in K562 leukemia cells unmasks ... This finding influenced the development of targeted drugs like Ibrutinib to treat leukemia and lymphoma. Witte's current ... the first targeted therapy for chronic myelogenous leukemia. Witte also co-discovered the gene for Bruton's tyrosine kinase, a ...
*  David Baltimore
Witte, Owen (28 February 1980). "Abelson murine leukemia virus protein is phosphorylated in vitro to form phosphotyrosine". ... Baltimore extended this work and examined two RNA tumor viruses, Rauscher murine leukemia virus and Rous sarcoma virus. He went ... his lab and intellectual interests expanded tackling new problems such as the pathogenesis of Abelson murine leukemia virus ( ... allowing such viruses to turn viral RNA strands into viral DNA strands. The viruses that fall into this category include HIV. ...
*  Clifford Tabin
... fibroblasts by cloned Abelson murine leukemia virus DNA and recovery of transmissible virus by recombination with helper virus ... In Weinberg's lab, Tabin constructed murine leukemia virus, the first recombinant retrovirus that could be used as a eukaryotic ... "Human EJ bladder carcinoma oncogene is homologue of Harvey sarcoma virus ras gene". Nature. 297 (5866): 474-8. doi:10.1038/ ...
*  Leukemia virus
Abelson murine leukemia virus Bovine leukemia virus Feline leukemia virus Human T-lymphotropic virus Murine leukemia virus ... Xenotropic murine leukemia virus-related virus Gibbon-ape leukemia virus Leucosis. ...
*  Abelson
... the mineral Abelson murine leukemia virus, also known as "Abelson's virus" Abelson's paradox. ... Alan Abelson (1925-2013), American journalist Hal Abelson, American computer scientist Hope Abelson, American artist and arts ... American Entrepreneur John Abelson (born 1939), American biochemist Matthew Abelson, American musician Neva Abelson (1910-2000 ... Abelson (1928-2005), American political scientist Sophie Abelson, an English stage and television actress Peter Abelsson (born ...
*  Murine leukemia virus
The Moloney, Rauscher, Abelson and Friend MLVs, named for their discoverers, are used in cancer research. Endogenous MLVs are ... The Friend virus (FV) is a strain of murine leukemia virus. The Friend virus has been used for both immunotherapy and vaccines ... The murine leukemia viruses (MLVs or MuLVs) are retroviruses named for their ability to cause cancer in murine (mouse) hosts. ... The murine leukemia viruses are group/type VI retroviruses belonging to the gammaretroviral genus of the Retroviridae family. ...
*  List of MeSH codes (B04)
... leukemia virus, gibbon ape MeSH B04.820.650.375.525 --- leukemia virus, murine MeSH B04.820.650.375.525.020 --- abelson murine ... abelson murine leukemia virus MeSH B04.909.574.807.375.525.050 --- akr murine leukemia virus MeSH B04.909.574.807.375.525.225 ... abelson murine leukemia virus MeSH B04.909.777.731.375.525.050 --- akr murine leukemia virus MeSH B04.909.777.731.375.525.225 ... akr murine leukemia virus MeSH B04.820.650.375.525.225 --- friend murine leukemia virus MeSH B04.820.650.375.525.300 --- gross ...
*  NSG mouse
"Dengue virus infection and virus-specific HLA-A2 restricted immune responses in humanized NOD-scid IL2rgammanull mice". PLoS ... Simpson-Abelson MR, Sonnenberg GF, Takita H, et al. (2008). "Long-term engraftment and expansion of tumor-derived memory T ... 2009). "Improved murine model of malaria using Plasmodium falciparum competent strains and non-myelodepleted NOD-scid ... Models of acute or chronic leukemia established using cancer cells collected from patients (A complete list of publications is ...
*  ABL2
"Entrez Gene: ABL2 v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene)". Nagy, Ádám; Pongor, ... Genes, abl at the US National Library of Medicine Medical Subject Headings (MeSH) Abelson Leukemia Virus at the US National ... The protein Abl gene is also known as abelson murine leukemia viral oncogene homolog 1 and is a protein that is encoded by the ... Tyrosine-protein kinase ABL2 also known as Abelson-related gene (Arg) is an enzyme that in humans is encoded by the ABL2 gene. ...
*  ABL (gene)
Abelson murine leukemia viral oncogene homolog 1 also known as ABL1 is a protein that, in humans, is encoded by the ABL1 gene ( ... Abelson Leukemia Virus at the US National Library of Medicine Medical Subject Headings (MeSH) Drosophila Abl tyrosine kinase - ... "Entrez Gene: ABL1 v-abl Abelson murine leukemia viral oncogene homolog 1". Tani K, Sato S, Sukezane T, Kojima H, Hirose H, ... a characteristic abnormality in chronic myelogenous leukemia (CML) and rarely in some other leukemia forms. The BCR-ABL ...
Abelson murine leukemia virus - Wikipedia  Abelson murine leukemia virus - Wikipedia
The Abelson murine leukemia virus (Ab-MLV or A-MuLV) is a retrovirus (Class VI) used to induce transformation of murine ... The Abelson murine leukemia virus is named for the American pediatrician Herbert T. Abelson, who first described and isolated ... Shields A, Rosenberg N, Baltimore D (1979). "Virus production by Abelson murine leukemia virus-transformed lymphoid cells". J. ... Abelson murine leukemia virus at the US National Library of Medicine Medical Subject Headings (MeSH). ...
more infohttps://en.wikipedia.org/wiki/Abelson_murine_leukemia_virus
Preparation of Syngeneic Tumor Regressor Serum Reactive with the Unique Determinants of the Abelson Murine Leukemia Virus...  Preparation of Syngeneic Tumor Regressor Serum Reactive with the Unique Determinants of the Abelson Murine Leukemia Virus...
Antisera reactive with the Abelson murine leukemia virus (A-MuLV)-specified P120 (anti-AbT sera) were produced in C57L/J mice. ... Preparation of Syngeneic Tumor Regressor Serum Reactive with the Unique Determinants of the Abelson Murine Leukemia Virus- ... Preparation of syngeneic tumor regressor serum reactive with the unique determinants of the Abelson murine leukemia virus- ... Preparation of Syngeneic Tumor Regressor Serum Reactive with the Unique Determinants of the Abelson Murine Leukemia Virus- ...
more infohttps://authors.library.caltech.edu/32771/
Abelson murine leukemia virus  Abelson murine leukemia virus
The Abelson murine leukemia virus (Ab-MLV, A-MuLV) is a retrovirus (Class VI) used to induce transformation of murine lymphoid ... The Abelson murine leukemia virus is named for the American pediatrician Herbert T. Abelson who first described and isolated it ... A highly efficient helper virus commonly used when growing A-MuLV in vitro is Moloney murine leukemia virus (M-MuLV). It causes ... A-MuLV causes a rapidly progressive lymphosarcoma known as Abelson disease in mice, which is a type of leukemia that does not ...
more infohttp://make-smart.blogspot.com/2010/03/abelson-murine-leukemia-virus_19.html
Transformation induced by Abelson murine leukemia virus involves production of a polypeptide growth factor. - Semantic Scholar  Transformation induced by Abelson murine leukemia virus involves production of a polypeptide growth factor. - Semantic Scholar
After purification to homogeneity, Abelson virus-induced TGF migrates as a single polypeptide with an apparent size of 7400 ... Transformation-defective mutants of Abelson MuLV do not transform cells, do not have their virus coded transforming gene ... Abelson MuLV-induced TGF morphologically transforms cells in culture, competes with 125I-labeled epidermal growth factor (EGF) ... is correlated with a tyrosine-specific protein kinase that is functionally active and is associated with the major Abelson MuLV ...
more infohttps://www.semanticscholar.org/paper/Transformation-induced-by-Abelson-murine-leukemia-a-Twardzik-Todaro/a73b87d9964bcfabba9601f526cdcee51713932b
JCI -
TYK2 is a key regulator of the surveillance of B lymphoid tumors  JCI - TYK2 is a key regulator of the surveillance of B lymphoid tumors
The single exposure to the Abelson murine leukemia virus (A-MuLV) induces a slowly evolving B lymphoid leukemia/lymphoma that ... Nonstandard abbreviations used: A-MuLV, Abelson murine leukemia virus; EC50, 50% effective concentration; MTT, 3-(4,5- ... The cell-autonomous properties of Abelson murine leukemia virus-transformed (A-MuLV-transformed) TYK2-/- cells were unaltered, ... Mice deficient in TYK2 developed Abelson-induced B lymphoid leukemia/lymphoma as well as TEL-JAK2-induced T lymphoid leukemia ...
more infohttps://www.jci.org/articles/view/22315
By Organization / Company Page 1  By Organization / Company Page 1
Cell Type: Macrophage; Abelson Murine Leukemia Virus Transformed .plsearchwithin .searchinresults input.text { padding: 7px 4px ... Tissue: Abelson Murine Leukemia Virus-Induced Tumor; Ascites Disease Abelson Murine Leukemia Virus-Induced Tumor ...
more infohttps://www.atcc.org/en/Products/Quality_Control_Strains/By_Organization__Company.aspx?dsNav=Ns:P_Product_Name%7C101%7C1%7C,Nrc:id-1000506-dynrank-disabled,N:4294925535&hasShowAll=1000506%7C
By Organization / Company Page 1  By Organization / Company Page 1
Cell Type: Macrophage; Abelson Murine Leukemia Virus Transformed .plsearchwithin .searchinresults input.text { padding: 7px 4px ... Tissue: Abelson Murine Leukemia Virus-Induced Tumor; Ascites Disease Abelson Murine Leukemia Virus-Induced Tumor ...
more infohttps://www.atcc.org/Products/Quality_Control_Strains/By_Organization__Company.aspx?dsNav=Ns:P_Product_Name%7C101%7C1%7C,Nrc:id-1000506-dynrank-disabled,N:4294925535&hasShowAll=1000506%7C&slp=1
By Organization / Company Page 1  By Organization / Company Page 1
Cell Type: Macrophage; Abelson Murine Leukemia Virus Transformed .plsearchwithin .searchinresults input.text { padding: 7px 4px ... Tissue: Abelson Murine Leukemia Virus-Induced Tumor; Ascites Disease Abelson Murine Leukemia Virus-Induced Tumor ...
more infohttps://atcc.org/Products/Quality_Control_Strains/By_Organization__Company.aspx?dsNav=Ns:P_Product_Name%7C101%7C1%7C,Nrc:id-1000506-dynrank-disabled,N:4294925535&hasShowAll=1000506%7C
Regulated expression of an immunoglobulin kappa gene introduced into a mouse lymphoid cell line   - CaltechAUTHORS  Regulated expression of an immunoglobulin kappa gene introduced into a mouse lymphoid cell line - CaltechAUTHORS
... gene into an Abelson murine leukemia virus-transformed lymphoid cell line. Plasmid pSV2gpt-κ41, containing the ĸ light chain ... We have introduced a functionally rearranged murine ĸ light chain immunoglobulin (Ig) ... Abelson murine leukemia virus; DNA transfection; gpt selection; lipopolysaccharide induction. Issue or Number:. 24. ...
more infohttps://authors.library.caltech.edu/9671/
2010 Distinguished Research Career Award | College of Veterinary Medicine  2010 Distinguished Research Career Award | College of Veterinary Medicine
He isolated the Abelson murine leukemia virus; identified the v-abl oncogene that is the cause of the viral cancer; and ... Goff has strategically and cleverly leveraged viruses to decipher how normal cells enable virus replication, yet defend against ... Goff's work led to realization that ABL is aberrantly expressed in chronic myelogenous leukemia in humans and led to the ... Goff developed the first methods to deliver recombinant DNA molecules into mammalian cells by employing SV40 virus, and was ...
more infohttps://vet.osu.edu/retrovirus-research/2010-distinguished-research-career-award
Recombinant Anti-CSDE1/NRU antibody [EPR17414] (ab201688) | Abcam  Recombinant Anti-CSDE1/NRU antibody [EPR17414] (ab201688) | Abcam
Lane 3 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus). Lysates/proteins at 10 µg per lane. ... Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) lysate. Lane 2 : HEK293 (Human embryonic kidney). ... CSDE1/NRU was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell ...
more infohttps://www.abcam.com/csde1nru-antibody-epr17414-ab201688.html
Recombinant Anti-Bim antibody [Y36] KO Tested (ab32158) | Abcam  Recombinant Anti-Bim antibody [Y36] KO Tested (ab32158) | Abcam
Lane 4 : Raw264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate. Lysates/proteins at 10 µg per lane.. ... Lane 3 : Molt-4 (human acute lymphoblastic leukemia) whole cell lysate. Lane 4 : Human thymus tissue lysate. Lane 5 : Mouse ...
more infohttps://www.abcam.com/bim-antibody-y36-ab32158.html
Cell Types Counted | Nexcelom Bioscience  Cell Types Counted | Nexcelom Bioscience
ascites: Abelson murine leukemia virus-induced tumor. MC38. Mouse. epithelial. colon adenocarcinoma. ...
more infohttps://www.nexcelom.com/training-and-support/cell-types-counted/
Whole Cell Lysates | Cell Lysates | ProSci Inc  Whole Cell Lysates | Cell Lysates | ProSci Inc
RAW 264.7 (Abelson murine leukemia virus-induced tumor) (1) * Rectum (6) * salivary gland (2) ...
more infohttps://www.prosci-inc.com/lysates.html?tissue_cell_type=512
Whole Cell Lysates | Cell Lysates | ProSci Inc  Whole Cell Lysates | Cell Lysates | ProSci Inc
RAW 264.7 (Abelson murine leukemia virus-induced tumor) (1) * Rectum (6) * salivary gland (2) ...
more infohttps://www.prosci-inc.com/lysates.html?tissue_cell_type=472
Temperature-Sensitive Transformation by an Abelson Virus Mutant Encoding an Altered SH2 Domain | Journal of Virology  Temperature-Sensitive Transformation by an Abelson Virus Mutant Encoding an Altered SH2 Domain | Journal of Virology
1976) A quantitative assay for transformation of bone marrow cells by Abelson murine leukemia virus. J. Exp. Med. 143:1453-1463 ... Abelson murine leukemia virus (Ab-MLV) encodes the v-Abl protein tyrosine kinase and induces transformation of immortalized ... Expression of the protein tyrosine kinase encoded by the v-abl oncogene of Abelson murine leukemia virus (Ab-MLV) induces ... 1990) Temperature-sensitive mutants of Abelson murine leukemia virus deficient in protein tyrosine kinase activity. J. Virol. ...
more infohttps://jvi.asm.org/content/75/4/1816
Analysis of ER-mitochondria contacts using correlative fluorescence microscopy and soft X-ray tomography of mammalian cells |...  Analysis of ER-mitochondria contacts using correlative fluorescence microscopy and soft X-ray tomography of mammalian cells |...
1987). Abelson murine leukemia virus-induced thymic lymphomas: transformation of a primitive lymphoid precursor. J. Natl. ...
more infohttp://jcs.biologists.org/content/128/15/2795
The Extreme Carboxyl Terminus of v-Abl Is Required for Lymphoid Cell Transformation by Abelson Virus | Journal of Virology  The Extreme Carboxyl Terminus of v-Abl Is Required for Lymphoid Cell Transformation by Abelson Virus | Journal of Virology
Abelson murine leukemia virus mutants with alterations in the virus-specific P120 molecule. J. Virol. 33:340-348. ... Abelson murine leukemia virus (Ab-MLV) is a replication-defective retrovirus that transforms pre-B cells and NIH 3T3 cells in ... Abelson murine leukemia virus mutants deficient in kinase activity and lymphoid cell transformation. J. Virol. 36:766-774. ... A quantitative assay for transformation of bone marrow cells by Abelson murine leukemia virus. J. Exp. Med. 143:1453-1463. ...
more infohttps://jvi.asm.org/content/77/8/4617
Peter K.  Jackson | Stanford Medicine Profiles  Peter K. Jackson | Stanford Medicine Profiles
c-abl is the normal cellular homolog of the v-abl transforming gene of Abelson murine leukemia virus. By constructing ... the v-abl oncogene of the Abelson murine leukemia virus by the replacement of their N-terminal sequences with viral gag ... Overexpression of p150c-abl type IV in a retroviral vector similar to Abelson virus does not transform NIH 3T3 fibroblasts, ... Our study uncovers a novel mechanism that RV employs to promote β-TrCP turnover and provides molecular insights into virus- ...
more infohttps://med.stanford.edu/profiles/peter-jackson
Bryostatin and Vincristine in B-Cell Malignancies  Bryostatin and Vincristine in B-Cell Malignancies
Abelson Murine Leukemia Virus. A replication-defective strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) capable of ... lymphoid cells and producing a rapidly progressing lymphoid leukemia after superinfection with FRIEND MURINE LEUKEMIA VIRUS; ... Transformation from promyelocytic leukemia with t (15; 17) ( q22; q21) to acute monocytic leukemia with t (11; 17) (q23; q21) ... To report on a case of therapy-related acute monocytic leukemia(t-AML) with t(11;17) (q23;q21)/MLL-AF17q after successful ...
more infohttps://www.bioportfolio.com/resources/trial/126311/Bryostatin-and-Vincristine-in-B-Cell-Malignancies.html
p19ARF Antibody (NB200-106): Novus Biologicals  p19ARF Antibody (NB200-106): Novus Biologicals
Unnikrishnan I, Rosenberg N. Absence of p53 complements defects in Abelson murine leukemia virus signaling. J Virol 77(11):6208 ... The absence of Msh2 alters abelson virus pre-B-cell transformation by influencing p53 mutation. Mol Cell Biol 2000 Nov [PMID: ... Proteins encoded by the INK4a/ARF locus also play a role in Abelson virus transformation. Both p16INK4a and p19ARF are ... Active Akt and functional p53 modulate apoptosis in Abelson virus-transformed pre-B cells. J Virol 78(4):1636-44. 2004 Feb. [ ...
more infohttps://www.novusbio.com/products/p19arf-antibody_nb200-106
Connection | Harvard Catalyst Profiles | Harvard Catalyst  Connection | Harvard Catalyst Profiles | Harvard Catalyst
Multiple immunoglobulin heavy-chain gene transcripts in Abelson murine leukemia virus-transformed lymphoid cell lines. ... Structure and expression of the murine L-myc gene. Academic Article Replacement of germ-line epsilon promoter by gene targeting ... Potential regulatory elements for germline transcription in or near murine Sgamma1. Academic Article Structure and expression ... Germline transcription and recombination of a murine VDJmudeltagamma1 transgene. Academic Article Transcription-induced ...
more infohttps://connects.catalyst.harvard.edu/Profiles/search/default.aspx?searchtype=whypeople&nodeuri=https://connects.catalyst.harvard.edu/Profiles/profile/1252168&searchfor=Transcription,%20Genetic&exactphrase=&perpage=15&offset=0&page=1&totalpages=161&searchrequest=&sortby=&sortdirection=&showcolumns=1
Table of Contents | Molecular and Cellular Biology  Table of Contents | Molecular and Cellular Biology
Multiple immunoglobulin heavy-chain gene transcripts in Abelson murine leukemia virus-transformed lymphoid cell lines. F W Alt ... Half-life of the Rous sarcoma virus transforming protein pp60src and its associated kinase activity. A Ziemiecki, R R Friis, H ... Adaptation of a retrovirus as a eucaryotic vector transmitting the herpes simplex virus thymidine kinase gene. C J Tabin, J W ...
more infohttps://mcb.asm.org/content/2/4
Exbio antibodies - Rat Monoclonal to CD45R (human, mouse) RA3-6B2 (IgG2a)  Exbio antibodies - Rat Monoclonal to CD45R (human, mouse) RA3-6B2 (IgG2a)
Abelson murine leukemia virus-induced pre-B tumor cells. Species Reactivity: *Human ... CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells. Entrez Gene: 1 E4; 1 ... Watanabe Y, Akaike T: Activation signal induces the expression of B cell-specific CD45R epitope (6B2) on murine T cells. Scand ... 1. Surface staining of CD45R in murine splenocytes with anti-CD45R (RA3-6B2) APC.. ...
more infohttp://www.exbio.cz/products/clone.py?idclone=CLO000000000000551