A Kinase Anchor Proteins: A structurally-diverse family of intracellular-signaling adaptor proteins that selectively tether specific protein kinase A subtypes to distinct subcellular sites. They play a role in focusing the PROTEIN KINASE A activity toward relevant substrates. Over fifty members of this family exist, most of which bind specifically to regulatory subunits of CYCLIC AMP-DEPENDENT PROTEIN KINASE TYPE II such as CAMP PROTEIN KINASE RIIALPHA or CAMP PROTEIN KINASE RIIBETA.Cyclic AMP-Dependent Protein Kinase RIIbeta Subunit: A type II cAMP-dependent protein kinase regulatory subunit that plays a role in confering CYCLIC AMP activation of protein kinase activity. It has a lower affinity for cAMP than the CYCLIC-AMP-DEPENDENT PROTEIN KINASE RIIALPHA SUBUNIT. Binding of this subunit by A KINASE ANCHOR PROTEINS may play a role in the cellular localization of type II protein kinase A.Cyclic AMP-Dependent Protein Kinase RIIalpha Subunit: A type II cAMP-dependent protein kinase regulatory subunit that plays a role in confering CYCLIC AMP activation of protein kinase activity. It has a higher affinity for cAMP than that of the CYCLIC-AMP-DEPENDENT PROTEIN KINASE RIIBETA SUBUNIT. Binding of this subunit by A KINASE ANCHOR PROTEINS may play a role in the cellular localization of type II protein kinase A.Cyclic AMP-Dependent Protein Kinases: A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition.Adaptor Proteins, Signal Transducing: A broad category of carrier proteins that play a role in SIGNAL TRANSDUCTION. They generally contain several modular domains, each of which having its own binding activity, and act by forming complexes with other intracellular-signaling molecules. Signal-transducing adaptor proteins lack enzyme activity, however their activity can be modulated by other signal-transducing enzymesCarrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Cyclic AMP-Dependent Protein Kinase Type II: A cyclic AMP-dependent protein kinase subtype primarily found in particulate subcellular fractions. They are tetrameric proteins that contain two catalytic subunits and two type II-specific regulatory subunits.Salivary alpha-Amylases: A subclass of alpha-amylase ISOENZYMES that are secreted into SALIVA.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Seminal Plasma Proteins: Proteins found in SEMEN. Major seminal plasma proteins are secretory proteins from the male sex accessory glands, such as the SEMINAL VESICLES and the PROSTATE. They include the seminal vesicle-specific antigen, an ejaculate clotting protein; and the PROSTATE-SPECIFIC ANTIGEN, a protease and an esterase.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Phosphatidylinositol 3-Kinases: Phosphotransferases that catalyzes the conversion of 1-phosphatidylinositol to 1-phosphatidylinositol 3-phosphate. Many members of this enzyme class are involved in RECEPTOR MEDIATED SIGNAL TRANSDUCTION and regulation of vesicular transport with the cell. Phosphatidylinositol 3-Kinases have been classified both according to their substrate specificity and their mode of action within the cell.MAP Kinase Signaling System: An intracellular signaling system involving the MAP kinase cascades (three-membered protein kinase cascades). Various upstream activators, which act in response to extracellular stimuli, trigger the cascades by activating the first member of a cascade, MAP KINASE KINASE KINASES; (MAPKKKs). Activated MAPKKKs phosphorylate MITOGEN-ACTIVATED PROTEIN KINASE KINASES which in turn phosphorylate the MITOGEN-ACTIVATED PROTEIN KINASES; (MAPKs). The MAPKs then act on various downstream targets to affect gene expression. In mammals, there are several distinct MAP kinase pathways including the ERK (extracellular signal-regulated kinase) pathway, the SAPK/JNK (stress-activated protein kinase/c-jun kinase) pathway, and the p38 kinase pathway. There is some sharing of components among the pathways depending on which stimulus originates activation of the cascade.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Protein Kinases: A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.Protein-Serine-Threonine Kinases: A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.
(1/496) The type and the localization of cAMP-dependent protein kinase regulate transmission of cAMP signals to the nucleus in cortical and cerebellar granule cells.

cAMP signals are received and transmitted by multiple isoforms of cAMP-dependent protein kinases, typically determined by their specific regulatory subunits. In the brain the major regulatory isoform RIIbeta and the RII-anchor protein, AKAP150 (rat) or 75 (bovine), are differentially expressed. Cortical neurons express RIIbeta and AKAP75; conversely, granule cerebellar cells express predominantly RIalpha and RIIalpha. Cortical neurons accumulate PKA catalytic subunit and phosphorylated cAMP responsive element binding protein very efficiently into nuclei upon cAMP induction, whereas granule cerebellar cells fail to do so. Down-regulation of RIIbeta synthesis by antisense oligonucleotides inhibited cAMP-induced nuclear signaling in cortical neurons. Expression in cerebellar granule cells of RIIbeta and AKAP75 genes by microinjection of specific expression vectors, markedly stimulated cAMP-induced transcription of the lacZ gene driven by a cAMP-responsive element promoter. These data indicate that the composition of PKA in cortical and granule cells underlies the differential ability of these cells to transmit cAMP signals to the nucleus.  (+info)

(2/496) Cloning and characterization of a cDNA encoding an A-kinase anchoring protein located in the centrosome, AKAP450.

A combination of protein kinase A type II (RII) overlay screening, database searches and PCR was used to identify a centrosomal A-kinase anchoring protein. A cDNA with an 11.7 kb open reading frame was characterized and found to correspond to 50 exons of genomic sequence on human chromosome 7q21-22. This cDNA clone encoded a 3908 amino acid protein of 453 kDa, that was designated AKAP450 (DDBJ/EMBL/GenBank accession No. AJ131693). Sequence comparison demonstrated that the open reading frame contained a previously characterized cDNA encoding Yotiao, as well as the human homologue of AKAP120. Numerous coiled-coil structures were predicted from AKAP450, and weak homology to pericentrin, giantin and other structural proteins was observed. A putative RII-binding site was identified involving amino acid 2556 of AKAP450 by mutation analysis combined with RII overlay and an amphipatic helix was predicted in this region. Immunoprecipitation of RII from RIPA-buffer extracts of HeLa cells demonstrated co-precipitation of AKAP450. By immunofluorecent labeling with specific antibodies it was demonstrated that AKAP450 localized to centrosomes. Furthermore, AKAP450 was shown to co-purify in centrosomal preparations. The observation of two mRNAs and several splice products suggests additional functions for the AKAP450 gene.  (+info)

(3/496) Association of the type 1 protein phosphatase PP1 with the A-kinase anchoring protein AKAP220.

The cyclic AMP (cAMP)-dependent protein kinase (PKA) and the type 1 protein phosphatase (PP1) are broad-specificity signaling enzymes with opposing actions that catalyze changes in the phosphorylation state of cellular proteins. Subcellular targeting to the vicinity of preferred substrates is a means of restricting the specificity of each enzyme [1] [2]. Compartmentalization of the PKA holoenzyme is mediated through association of the regulatory subunits with A-kinase anchoring proteins (AKAPs), whereas a diverse family of phosphatase-targeting subunits directs the location of the PP1 catalytic subunit (PP1c) [3] [4]. Here, we demonstrate that the PKA-anchoring protein, AKAP220, binds PP1c with a dissociation constant (KD) of 12.1 +/- 4 nM in vitro. Immunoprecipitation of PP1 from cell extracts resulted in a 10.4 +/- 3.8-fold enrichment of PKA activity. AKAP220 co-purified with PP1c by affinity chromatography on microcystin sepharos Immunocytochemical analysis demonstrated that the kinase, the phosphatase and the anchoring protein had distinct but overlapping staining patterns in rat hippocampal neurons. Collectively, these results provide the first evidence that AKAP220 is a multivalent anchoring protein that maintains a signaling scaffold of PP1 and the PKA holoenzyme.  (+info)

(4/496) NH2-Terminal targeting motifs direct dual specificity A-kinase-anchoring protein 1 (D-AKAP1) to either mitochondria or endoplasmic reticulum.

Subcellular localization directed by specific targeting motifs is an emerging theme for regulating signal transduction pathways. For cAMP-dependent protein kinase (PKA), this is achieved primarily by its association with A-kinase-anchoring proteins (AKAPs). Dual specificity AKAP1, (D-AKAP1) binds to both type I and type II regulatory subunits and has two NH2-terminal (N0 and N1) and two COOH-terminal (C1 and C2) splice variants (. J. Biol. Chem. 272:8057). Here we report that the splice variants of D-AKAP1 are expressed in a tissue-specific manner with the NH2-terminal motifs serving as switches to localize D-AKAP1 at different sites. Northern blots showed that the N1 splice is expressed primarily in liver, while the C1 splice is predominant in testis. The C2 splice shows a general expression pattern. Microinjecting expression constructs of D-AKAP1(N0) epitope-tagged at either the NH2 or the COOH terminus showed their localization to the mitochondria based on immunocytochemistry. Deletion of N0(1-30) abolished mitochondrial targeting while N0(1-30)-GFP localized to mitochondria. Residues 1-30 of N0 are therefore necessary and sufficient for mitochondria targeting. Addition of the 33 residues of N1 targets D-AKAP1 to the ER and residues 1-63 fused to GFP are necessary and sufficient for ER targeting. Residues 14-33 of N1 are especially important for targeting to ER; however, residues 1-33 alone fused to GFP gave a diffuse distribution. N1(14-33) thus serves two functions: (a) it suppresses the mitochondrial-targeting motif located within residues 1-30 of N0 and (b) it exposes an ER-targeting motif that is at least partially contained within the N0(1-30) motif. This represents the first example of a differentially targeted AKAP and adds an additional level of complexity to the PKA signaling network.  (+info)

(5/496) Characterization of a novel giant scaffolding protein, CG-NAP, that anchors multiple signaling enzymes to centrosome and the golgi apparatus.

A novel 450-kDa coiled-coil protein, CG-NAP (centrosome and Golgi localized PKN-associated protein), was identified as a protein that interacted with the regulatory region of the protein kinase PKN, having a catalytic domain homologous to that of protein kinase C. CG-NAP contains two sets of putative RII (regulatory subunit of protein kinase A)-binding motif. Indeed, CG-NAP tightly bound to RIIalpha in HeLa cells. Furthermore, CG-NAP was coimmunoprecipitated with the catalytic subunit of protein phosphatase 2A (PP2A), when one of the B subunit of PP2A (PR130) was exogenously expressed in COS7 cells. CG-NAP also interacted with the catalytic subunit of protein phosphatase 1 in HeLa cells. Immunofluorescence analysis of HeLa cells revealed that CG-NAP was localized to centrosome throughout the cell cycle, the midbody at telophase, and the Golgi apparatus at interphase, where a certain population of PKN and RIIalpha were found to be accumulated. These data indicate that CG-NAP serves as a novel scaffolding protein that assembles several protein kinases and phosphatases on centrosome and the Golgi apparatus, where physiological events, such as cell cycle progression and intracellular membrane traffic, may be regulated by phosphorylation state of specific protein substrates.  (+info)

(6/496) Regulation of NMDA receptors by an associated phosphatase-kinase signaling complex.

Regulation of N-methyl-D-aspartate (NMDA) receptor activity by kinases and phosphatases contributes to the modulation of synaptic transmission. Targeting of these enzymes near the substrate is proposed to enhance phosphorylation-dependent modulation. Yotiao, an NMDA receptor-associated protein, bound the type I protein phosphatase (PP1) and the adenosine 3',5'-monophosphate (cAMP)-dependent protein kinase (PKA) holoenzyme. Anchored PP1 was active, limiting channel activity, whereas PKA activation overcame constitutive PP1 activity and conferred rapid enhancement of NMDA receptor currents. Hence, yotiao is a scaffold protein that physically attaches PP1 and PKA to NMDA receptors to regulate channel activity.  (+info)

(7/496) Conservation and function of a bovine sperm A-kinase anchor protein homologous to mouse AKAP82.

Protein kinase A regulates sperm motility through the cAMP-dependent phosphorylation of proteins. One mechanism to direct the activity of the kinase is to localize it near its protein substrates through the use of anchoring proteins. A-Kinase anchoring proteins (AKAPs) act by binding the type II regulatory subunit of protein kinase A and tethering it to a cellular organelle or cytoskeletal element. We showed previously that mAKAP82, the major protein of the fibrous sheath of the mouse sperm flagellum, is an AKAP. The available evidence indicates that protein kinase A is compartmentalized to the fibrous sheath by binding mAKAP82. To characterize AKAP82 in bovine sperm, a testicular cDNA library was constructed and used to isolate a clone encoding bAKAP82, the bovine homologue. Sequence analysis showed that the primary structure of bAKAP82 was highly conserved. In particular, the amino acid sequence corresponding to the region of mAKAP82 responsible for binding the regulatory subunit of protein kinase A was identical in the bull. Bovine AKAP82 was present in both epididymal and ejaculated sperm and was localized to the entire principal piece of the flagellum, the region in which the fibrous sheath is located. Finally, bAKAP82 bound the regulatory subunit of protein kinase A. These data support the idea that bAKAP82 functions as an anchoring protein for the subcellular localization of protein kinase A in the flagellum.  (+info)

(8/496) mAKAP: an A-kinase anchoring protein targeted to the nuclear membrane of differentiated myocytes.

The compartmentalization of second messenger-activated protein kinases contributes to the fidelity of hormone-mediated signal transduction events. For example, the cAMP-dependent protein kinase is tethered at specific intracellular locations through association with A-kinase anchoring proteins (AKAPs). We now report the cloning of mAKAP, an anchoring protein found predominantly in heart, skeletal muscle and brain, and whose expression is induced in neonatal ventriculocytes by treatment with hypertrophic stimuli. mAKAP is targeted to the nuclear membrane of differentiated myocytes. Analysis of mAKAP-green fluorescent protein (GFP) fusion constructs revealed that nuclear membrane targeting is conferred by two regions of the protein, between residues 772-915 and 915-1065, which contain spectrin-like repeat sequences. Heterologous expression of the mAKAP targeting sequences displaced the endogenous anchoring protein from the nuclear membrane, demonstrating that mAKAP targeting is saturable. Collectively, these data suggest that a domain containing spectrin-like repeats mediates targeting of the anchoring protein mAKAP and the cAMP-dependent protein kinase holoenzyme to the nuclear membrane in response to differentiation signals.  (+info)

*  Protein primary structure
... atom of cysteine residues to anchor proteins to cellular membranes. Unlike the GPI and myritoyl anchors, these groups are not ... Such reactions are catalyzed by kinases and the reverse reaction is catalyzed by phosphatases. The phosphorylated tyrosines are ... Protein primary structure is the linear sequence of amino acids in a peptide or protein. By convention, the primary structure ... The protein is activated by cleaving off the inhibitory peptide. Some proteins even have the power to cleave themselves. ...
*  A-kinase-anchoring protein
The A-kinase anchoring proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding ... AKAPs act as scaffold proteins wherein they bind PKA and other signaling proteins and physically tether these multi-protein ... "A-kinase-anchoring proteins". Journal of Cell Science. 118 (15): 3217-3220. doi:10.1242/jcs.02416. Schwartz JH (November 2001 ... to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. At ...
*  LDB3
"Cypher/ZASP is a novel A-kinase anchoring protein". The Journal of Biological Chemistry. 288 (41): 29403-13. doi:10.1074/jbc. ... "Protein-protein interaction of zinc finger LIM domains with protein kinase C". The Journal of Biological Chemistry. 271 (49): ... ZASP is a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino ... a striated muscle-restricted PDZ and LIM domain-containing protein, binds to alpha-actinin-2 and protein kinase C". The Journal ...
*  AKAP6
A-kinase anchor protein 6 is an enzyme that in humans is encoded by the AKAP6 gene. The A-kinase anchor proteins (AKAPs) are a ... "Cloning and characterization of A-kinase anchor protein 100 (AKAP100). A protein that targets A-kinase to the sarcoplasmic ... "Entrez Gene: AKAP6 A kinase (PRKA) anchor protein 6". Marx, S O; Reiken S; Hisamatsu Y; Jayaraman T; Burkhoff D; Rosemblit N; ... Kapiloff MS, Schillace RV, Westphal AM, Scott JD (1999). "mAKAP: an A-kinase anchoring protein targeted to the nuclear membrane ...
*  Golgi matrix
Chen L, Marquardt ML, Tester DJ, Sampson KJ, Ackerman MJ, Kass RS (2007). "Mutation of an A-kinase-anchoring protein causes ... Golgi reassembly-stacking proteins are an evolutionarily conserved family of proteins in the Golgi matrix. GRASP65 and GRASP55 ... encoding a transmembrane protein with a presumed role in vesicle-mediated sorting and intracellular protein transport". Am. J. ... The first individual protein component of the matrix was identified in 1995 as Golgin A2 (then called GM130). Since then, many ...
*  LRBA
... evolutionarily conserved human gene family encoding proteins with presumptive protein kinase A anchoring function". Genomics. ... LRBA contains key features of both beige/CHS1 and A kinase anchor proteins (AKAPs; see MIM 602449).[supplied by OMIM] GRCh38: ... of a novel lipopolysaccharide-inducible gene with key features of both A kinase anchor proteins and chs1/beige proteins". J ... Lipopolysaccharide-responsive and beige-like anchor protein is a protein that in humans is encoded by the LRBA gene. Patients ...
*  AKAP7
This gene encodes a member of the A-kinase anchoring protein (AKAP) family, a group of functionally related proteins that bind ... "A novel lipid-anchored A-kinase Anchoring Protein facilitates cAMP-responsive membrane events". The EMBO Journal. 17 (8): 2261- ... "Bioinformatic design of A-kinase anchoring protein-in silico: a potent and selective peptide antagonist of type II protein ... A-kinase anchor protein 7 isoform gamma is an enzyme that in humans is encoded by the AKAP7 gene. ...
*  SPA17
Lea IA, Widgren EE, O'Rand MG (2004). "Association of sperm protein 17 with A-kinase anchoring protein 3 in flagella". Reprod. ... Frayne J, Hall L (2003). "A re-evaluation of sperm protein 17 (Sp17) indicates a regulatory role in an A-kinase anchoring ... Sperm surface protein Sp17 is a protein that in humans is encoded by the SPA17 gene. This gene encodes a protein present at the ... Studies in rabbits suggest that in sperm the protein is involved in fertilization by binding to the zona pellucida of the ...
*  Mohamed Ali Al-Shaaban
Bernard Abrenica; Mohamed AlShaaban; Michael P Czubryt (May 2009). "The A-kinase anchor protein AKAP121 is a negative regulator ... "Induction of cardiomyocyte hypertrophy by specific knockdown of an a-kinase anchor protein". Journal of molecular and cellular ...
*  PRKACA
A-Kinase Anchoring Proteins (AKAPs) bind a surface formed between both R subunits and target the kinase to different locations ... Snyder EM, Colledge M, Crozier RA, Chen WS, Scott JD, Bear MF (Apr 2005). "Role for A kinase-anchoring proteins (AKAPS) in ... Protein kinase A catalytic subunit (PKA Cα) is a member of the AGC kinase family, and contributes to the control of cellular ... This led to the hypothesis that the subcellular localization of anchored PKA controls what proteins are regulated by the kinase ...
*  Self-incompatibility
Another protein essential for the SI response is MLPK, a serine-threonine kinase, which is anchored to the plasma membrane from ... "A membrane-anchored protein kinase involved in Brassica self-incompatibility signaling". Science. 303 (5663): 1516-9. doi: ... The female determinant of the SI response in Brassica, is a transmembrane protein termed SRK, which has an intracellular kinase ... The interaction between the SRK and SCR/SP11 proteins results in autophosphorylation of the intracellular kinase domain of SRK ...
*  Mir-186 microRNA precursor family
"Down-regulation of tumor suppressor a kinase anchor protein 12 in human hepatocarcinogenesis by epigenetic mechanisms". ...
*  AKAP4
A-kinase anchor protein 4 is a scaffold protein that in humans is encoded by the AKAP4 gene. The A-kinase anchor proteins ( ... Brown PR, Miki K, Harper DB, Eddy EM (June 2003). "A-kinase anchoring protein 4 binding proteins in the fibrous sheath of the ... Brown PR, Miki K, Harper DB, Eddy EM (June 2003). "A-kinase anchoring protein 4 binding proteins in the fibrous sheath of the ... Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation". ...
*  Synaptophysin
Ulfig N, Chan WY (2003). "Expression of a kinase anchoring protein 79 and synaptophysin in the developing human red nucleus". ... The encoded protein has 313 amino acids with a predicted molecular weight of 33.845 kDa. The protein is a synaptic vesicle ... Synaptophysin, also known as the major synaptic vesicle protein p38, is a protein that in humans is encoded by the SYP gene. ... The exact function of the protein is unknown: it interacts with the essential synaptic vesicle protein synaptobrevin, but when ...
*  Tudor domain
Other tudor domain containing proteins include AKAP1 (A-kinase anchor protein 1) and ARID4A (AT rich interactive domain 4A) ... December 2006). "The KH-Tudor domain of a-kinase anchoring protein 149 mediates RNA-dependent self-association". Biochemistry. ... The proteins TP53BP1 (Tumor suppressor p53-binding protein 1) and its fission yeast homolog Crb2 and JMJD2A (Jumonji domain ... In molecular biology a tudor domain is a conserved protein structural domain originally identified as a region of 50 amino ...
*  Anil Suri
PMID 27057472 A-kinase anchor protein 4 (AKAP4) a promising therapeutic target of colorectal cancer. Jagadish N, Parashar D, ... PMID 20564126 Gene silencing of A-kinase anchor protein 4 inhibits cervical cancer growth in vitro and in vivo. Saini S, ... PMID 19190149 A novel cancer testis antigen, A-kinase anchor protein 4 (AKAP4) is a potential biomarker for breast cancer. ... PMID 17332284 A novel cancer testis antigen, A-kinase anchor protein 4 (AKAP4) is a putative target for immunotherapy of ...
*  C6orf10
Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation". J ... Uncharacterized protein C6orf10 is a protein that in humans is encoded by the C6orf10 gene. ENSG00000236672, ENSG00000204296, ...
*  Sperm-associated antigen 1
Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation". ... Furthermore, immunization of female rats with the recombinant human protein reduced fertility. This protein localizes to the ... interacts with G-protein beta 1 subunit and activates extracellular signal-regulated kinases (ERK)". Frontiers in Bioscience. ... The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman. ...
*  AKAP5
A-kinase anchor protein 5 is a protein that in humans is encoded by the AKAP5 gene. The A-kinase anchor proteins (AKAPs) are a ... 1995). "Association of protein kinase A and protein phosphatase 2B with a common anchoring protein". Science. 267 (5194): 108- ... "A-kinase anchoring protein 79/150 facilitates the phosphorylation of GABA(A) receptors by cAMP-dependent protein kinase via ... "Localization of the cAMP-dependent protein kinase to the postsynaptic densities by A-kinase anchoring proteins. ...
*  AKAP8
A-kinase anchor protein 8 is an enzyme that, in humans, is encoded by the AKAP8 gene. The A-kinase anchor proteins (AKAPs) are ... Collas P, Le Guellec K, Taskén K (Dec 1999). "The A-kinase-anchoring protein AKAP95 is a multivalent protein with a key role in ... 2003). "Protein kinase A-anchoring protein AKAP95 interacts with MCM2, a regulator of DNA replication". J. Biol. Chem. 278 (29 ... Collas P, Le Guellec K, Taskén K (2000). "The A-kinase-anchoring protein AKAP95 is a multivalent protein with a key role in ...
*  AKAP9
A-kinase anchor protein 9 is a protein that in humans is encoded by the AKAP9 gene. The A-kinase anchor proteins (AKAPs) are a ... These signaling proteins include type II protein kinase A, serine/threonine kinase protein kinase N, protein phosphatase 1, ... a multiply spliced protein kinase A-anchoring protein associated with centrosomes". J. Biol. Chem. 274 (5): 3055-66. doi: ... "Bioinformatic design of A-kinase anchoring protein-in silico: a potent and selective peptide antagonist of type II protein ...
*  AKAP8L
A-kinase anchor protein 8-like is a protein that in humans is encoded by the AKAP8L gene. AKAP8L has been shown to interact ... cloning and characterization of a novel nuclear protein, HA95, homologous to A-kinase anchoring protein 95". Biology of the ... "Entrez Gene: AKAP8L A kinase (PRKA) anchor protein 8-like". Yang JP, Tang H, Reddy TR, Wong-Staal F (Aug 2001). "Mapping the ... neighbor of A-kinase anchoring protein 95 (AKAP95) on chromosome 19p13.11-p13.12 region". Journal of Human Genetics. 45 (1): ...
*  DDX5
2001). "A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix and associates with p68 RNA helicase". J. Biol. ... "A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix and associates with p68 RNA helicase". J. Biol. Chem. 276 ( ... Buelt MK, Glidden BJ, Storm DR (1994). "Regulation of p68 RNA helicase by calmodulin and protein kinase C". J. Biol. Chem. 269 ... DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated ...
*  Sperm-associated antigen 6
Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation". ... a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar ... The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the ... "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome ...
*  AKAP13
A-kinase anchor protein 13 is an enzyme that in humans is encoded by the AKAP13 gene. The A-kinase anchor proteins (AKAPs) are ... the first protein kinase A anchoring protein to integrate protein kinase A and Rho signaling". FEBS Lett. 507 (3): 264-8. doi: ... of the cAMP-dependent protein kinase interaction with A-kinase anchor proteins requires isoleucines 3 and 5". J. Biol. Chem. ... function as protein kinase A-anchoring proteins. AKAP13 has been shown to interact with Estrogen receptor alpha, CTNNAL1 and ...
*  RHOB
Diviani D, Soderling J, Scott JD (2001). "AKAP-Lbc anchors protein kinase A and nucleates Galpha 12-selective Rho-mediated ... Identification of a non-cytosolic GDI protein interacting with the small GTP-binding proteins RhoB and RhoG". J. Biol. Chem. ... Identification of a non-cytosolic GDI protein interacting with the small GTP-binding proteins RhoB and RhoG". J. Biol. Chem. ... Ras homolog gene family, member B, also known as RHOB, is a protein which in humans is encoded by the RHOB gene. RHOB is a ...
*  AKAP2
2003). "Amino acid variant in the kinase binding domain of dual-specific A kinase-anchoring protein 2: a disease susceptibility ... "Bioinformatic design of A-kinase anchoring protein-in silico: a potent and selective peptide antagonist of type II protein ... "Bioinformatic design of A-kinase anchoring protein-in silico: a potent and selective peptide antagonist of type II protein ... A-kinase anchor protein 2 is an enzyme that in humans is encoded by the AKAP2 gene. AKAP2 has been shown to interact with ...
Abstract 1231: Competitive Displacement of A Kinase Anchor Protein 121 From Mitochondria Triggers DNA Damage And Lowers...  Abstract 1231: Competitive Displacement of A Kinase Anchor Protein 121 From Mitochondria Triggers DNA Damage And Lowers...
cAMP signals are locally amplified by scaffold proteins (A Kinase Anchor Proteins, AKAPs) that tether cAMP-dependent Protein ... Abstract 1231: Competitive Displacement of A Kinase Anchor Protein 121 From Mitochondria Triggers DNA Damage And Lowers ... Abstract 1231: Competitive Displacement of A Kinase Anchor Protein 121 From Mitochondria Triggers DNA Damage And Lowers ... Abstract 1231: Competitive Displacement of A Kinase Anchor Protein 121 From Mitochondria Triggers DNA Damage And Lowers ...
more infohttp://circ.ahajournals.org/content/116/Suppl_16/II_250.2
Splicing factor arginine/serine-rich 17A (SFRS17A) is an A-kinase anchoring protein that targets protein kinase A to splicing...  Splicing factor arginine/serine-rich 17A (SFRS17A) is an A-kinase anchoring protein that targets protein kinase A to splicing...
Protein kinase A (PKA) is targeted to distinct subcellular localizations by specific protein kinase A anchoring proteins (AKAPs ... Splicing factor arginine/serine-rich 17A (SFRS17A) is an A-kinase anchoring protein that targets protein kinase A to splicing ... Splicing factor arginine/serine-rich 17A (SFRS17A) is an A-kinase anchoring protein that targets protein kinase A to splicing ... we show by a variety of protein interaction assays that SFRS17A binds both type I and type II PKA in vitro and inside cells, ...
more infohttps://passport.as.uky.edu/splicing-factor-arginineserine-rich-17a-sfrs17a-kinase-anchoring-protein-targets-protein-kinase
Akap10 (untagged) - Mouse A kinase (PRKA) anchor protein 10 (Akap10), nuclear gene encoding mitochondrial protein, (10ug) -...  Akap10 (untagged) - Mouse A kinase (PRKA) anchor protein 10 (Akap10), nuclear gene encoding mitochondrial protein, (10ug) -...
... anchor protein 10 (Akap10), nuclear gene encoding mitochondrial protein, (10ug), 10 µg. ... MC220256 Akap10 (untagged) - Mouse A kinase (PRKA) anchor protein 10 (Akap10), nuclear gene encoding mitochondrial protein, ( ... Home » cDNA » Mouse cDNA » Akap10 (untagged) - Mouse A kinase (PRKA) anchor protein 10 (Akap10), nuclear gene encoding ... Properties for Akap10 (untagged) - Mouse A kinase (PRKA) anchor protein 10 (Akap10), nuclear gene encoding mitochondrial ...
more infohttps://www.acris-antibodies.com/cdna/mouse-cdna/akap10-untagged-mouse-a-kinase-prka-anchor-protein-10-akap10-nuclear-gene-encoding-mitochondrial-protein-10ug-mc220256.htm
A-kinase anchor protein 4 (AKAP4) a promising therapeutic target of colorectal cancer | Journal of Experimental & Clinical...  A-kinase anchor protein 4 (AKAP4) a promising therapeutic target of colorectal cancer | Journal of Experimental & Clinical...
A-kinase anchor protein 4 (AKAP4) gene and protein expression was monitored by quantitative polymerase chain reaction (qPCR), ... Gene silencing of A-kinase anchor protein 4 inhibits cervical cancer growth in vitro and in vivo. Cancer Gene Ther. 2013;1-8. ... Saini S, Jagadish N, Gupta A, Bhatnagar A, Suri A. A novel cancer testis antigen, A-kinase anchor protein 4 (AKAP4) is a ... Expression and humoral response of A-kinase anchor protein 4 in cervical cancer. Int J Gynecol Cancer. 2013;4:650-8.View ...
more infohttps://jeccr.biomedcentral.com/articles/10.1186/s13046-015-0258-y
Pain modulators regulate the dynamics of PKA-RII phosphorylation in subgroups of sensory neurons | Journal of Cell Science  Pain modulators regulate the dynamics of PKA-RII phosphorylation in subgroups of sensory neurons | Journal of Cell Science
2010). Specificity and spatial dynamics of protein kinase A signaling organized by A-kinase-anchoring proteins. J. Mol. ... 2008). A-kinase anchoring protein mediates TRPV1 thermal hyperalgesia through PKA phosphorylation of TRPV1. Pain 138, 604-616. ... 2008). Protein kinase A anchoring via AKAP150 is essential for TRPV1 modulation by forskolin and prostaglandin E2 in mouse ... 1997). Compensatory regulation of RIalpha protein levels in protein kinase A mutant mice. J. Biol. Chem. 272, 3993-3998. doi: ...
more infohttp://jcs.biologists.org/content/127/1/216
Human-gpDB  Human-gpDB
Homo sapiens protein kinase A anchoring protein Ht31 mRNA, complete cds. AF406992. Homo sapiens A-kinase anchoring protein mRNA ... RecName: Full=A-kinase anchor protein 13; Short=AKAP-13;AltName: Full=Protein kinase A-anchoring protein 13;AltName: Full= ... RecName: Full=A-kinase anchor protein 13; Short=AKAP 13; AltName: Full=Protein kinase A-anchoring protein 13; AltName: Full= ... A-kinase anchor protein 13 (AKAP 13) (Protein kinase A-anchoring protein 13) (Breast cancer nuclear receptor-binding auxiliary ...
more infohttp://schneider.embl.de/cgi-bin/human_gpdb.cgi?section=summary_sheet&gene=ENSG00000170776&category=effector&id=31
A-kinase-anchoring protein - Wikipedia  A-kinase-anchoring protein - Wikipedia
The A-kinase anchoring proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding ... AKAPs act as scaffold proteins wherein they bind PKA and other signaling proteins and physically tether these multi-protein ... "A-kinase-anchoring proteins". Journal of Cell Science. 118 (15): 3217-3220. doi:10.1242/jcs.02416. Schwartz JH (November 2001 ... to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. At ...
more infohttps://en.wikipedia.org/wiki/A-kinase-anchoring_protein
Function to Failure: Compartmentalization of Cardiomyocyte Signaling by A-Kinase-Anchoring Proteins | SpringerLink  Function to Failure: Compartmentalization of Cardiomyocyte Signaling by A-Kinase-Anchoring Proteins | SpringerLink
Critical role of cAMP-dependent protein kinase anchoring to the L-type calcium channel Cav1.2 via A-kinase anchor protein 150 ... McConnell BK, Popovic Z, Mai N et al (2009) Disruption of protein kinase a interaction with A-kinase-anchoring proteins in the ... Chen L, Kurokawa J, Kass RS (2005) Phosphorylation of the A-kinase-anchoring protein Yotiao contributes to protein kinase A ... Cloning and characterization of A-kinase anchor protein 100 (AKAP100). A protein that targets A-kinase to the sarcoplasmic ...
more infohttps://link.springer.com/chapter/10.1007/978-3-319-54579-0_3
AKAP9 A-kinase anchoring protein 9 [Homo sapiens (human)] - Gene - NCBI  AKAP9 A-kinase anchoring protein 9 [Homo sapiens (human)] - Gene - NCBI
A-kinase anchor protein 9. Names. A kinase (PRKA) anchor protein (yotiao) 9. A kinase (PRKA) anchor protein 9. A-kinase anchor ... kinase N-associated protein. protein hyperion. protein kinase A anchoring protein 9. protein phosphatase 1, regulatory subunit ... These signaling proteins include type II protein kinase A, serine/threonine kinase protein kinase N, protein phosphatase 1, ... AKAP9 A-kinase anchoring protein 9 [Homo sapiens] AKAP9 A-kinase anchoring protein 9 [Homo sapiens]. Gene ID:10142 ...
more infohttps://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=full_report&list_uids=10142
A-kinase anchoring protein 7 ELISA Kits | Biocompare.com  A-kinase anchoring protein 7 ELISA Kits | Biocompare.com
Compare A-kinase anchoring protein 7 ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, ... A-kinase anchoring protein 7 ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established antibody-based ... Human A-kinase anchor protein 7 isoforms alpha and beta, AKAP7 ELISA Kit ... Bovine A-kinase anchor protein 7 isoforms alpha and beta (AKAP7) ELISA Kit ...
more infohttps://www.biocompare.com/pfu/110627/soids/2-2273697/ELISA_Kit/ELISA_A-kinase_anchoring_protein_7
A-kinase anchoring protein 10 ELISA Kits | Biocompare.com  A-kinase anchoring protein 10 ELISA Kits | Biocompare.com
Compare A-kinase anchoring protein 10 ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, ... A-kinase anchoring protein 10 ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established antibody-based ... Your search returned 26 A-kinase anchoring protein 10 ELISA ELISA Kit across 5 suppliers. ...
more infohttps://www.biocompare.com/pfu/110627/soids/2-2269422/ELISA_Kit/ELISA_A-kinase_anchoring_protein_10
Organizing signal transduction through A-kinase anchoring proteins (AKAPs)  Organizing signal transduction through A-kinase anchoring proteins (AKAPs)
Anchoring, scaffolding and adapter proteins function to enhance the precision and directionality of these signaling events by ... A fundamental role for protein-protein interactions in the organization of signal transduction pathways is evident. ... The cAMP signaling pathway is organized by A-kinase anchoring proteins. This family of proteins assembles enzyme complexes ... Selected A-kinase anchoring protein signaling complexes are highlighted in this minireview. ...
more infohttps://insights.ovid.com/febs/febsj/2010/11/010/organizing-signal-transduction-kinase-anchoring/4/01217111
Signaling at A-Kinase Anchoring Proteins Organizes Anesthesia-Sensitive Memory in Drosophila | Journal of Neuroscience  Signaling at A-Kinase Anchoring Proteins Organizes Anesthesia-Sensitive Memory in Drosophila | Journal of Neuroscience
Signaling at A-Kinase Anchoring Proteins Organizes Anesthesia-Sensitive Memory in Drosophila. Martin Schwaerzel, Andrea Jaeckel ... Signaling at A-Kinase Anchoring Proteins Organizes Anesthesia-Sensitive Memory in Drosophila ... Signaling at A-Kinase Anchoring Proteins Organizes Anesthesia-Sensitive Memory in Drosophila ... Signaling at A-Kinase Anchoring Proteins Organizes Anesthesia-Sensitive Memory in Drosophila ...
more infohttps://www.jneurosci.org/content/27/5/1229/tab-e-letters
Frontiers | Targeting protein-protein interactions in complexes organized by A kinase anchoring proteins | Pharmacology  Frontiers | Targeting protein-protein interactions in complexes organized by A kinase anchoring proteins | Pharmacology
A-kinase anchoring proteins (AKAPs) directly bind PKA and recruit it to specific subcellular loci targeting the kinase activity ... A-kinase anchoring proteins (AKAPs) directly bind PKA and recruit it to specific subcellular loci targeting the kinase activity ... Here, we will focus on mechanisms for targeting protein-protein interaction, disruptors that modulate down-stream cAMP ... Here, we will focus on mechanisms for targeting protein-protein interaction, disruptors that modulate down-stream cAMP ...
more infohttps://www.frontiersin.org/articles/10.3389/fphar.2015.00192/full
JCDD | Free Full-Text | Roles of A-Kinase Anchoring Proteins and Phosphodiesterases in the Cardiovascular System  JCDD | Free Full-Text | Roles of A-Kinase Anchoring Proteins and Phosphodiesterases in the Cardiovascular System
... pathophysiological role as well as highlights the potential benefits of targeting these proteins and their protein-protein ... A-kinase anchoring proteins (AKAPs) and cyclic nucleotide phosphodiesterases (PDEs) are essential enzymes in the cyclic ... "Roles of A-Kinase Anchoring Proteins and Phosphodiesterases in the Cardiovascular System." J. Cardiovasc. Dev. Dis. 5, no. 1: ... Roles of A-Kinase Anchoring Proteins and Phosphodiesterases in the Cardiovascular System. Maria Ercu 1. ...
more infohttps://www.mdpi.com/2308-3425/5/1/14
Akap1 - A-kinase anchor protein 1, mitochondrial precursor - Mus musculus (Mouse) - Akap1 gene & protein  Akap1 - A-kinase anchor protein 1, mitochondrial precursor - Mus musculus (Mouse) - Akap1 gene & protein
Anchors them to the cytoplasmic face of the mitochondrial outer membrane or allows them to reside in the endoplasmic reticulum ... Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A. ... Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A. Anchors them to the ... "Identification of a novel protein kinase A anchoring protein that binds both type I and type II regulatory subunits.". Huang L. ...
more infohttps://www.uniprot.org/uniprot/O08715
A-Kinase Anchoring Protein Targeting of Protein Kinase A and Regulation of HERG Channels, The Journal of Membrane Biology | 10...  A-Kinase Anchoring Protein Targeting of Protein Kinase A and Regulation of HERG Channels, The Journal of Membrane Biology | 10...
"A-Kinase Anchoring Protein Targeting of Protein Kinase A and Regulation of HERG Channels, The Journal of Membrane Biology" on ... A-Kinase Anchoring Protein Targeting of Protein Kinase A and Regulation of HERG Channels. A-Kinase Anchoring Protein Targeting ... A-Kinase Anchoring Protein Targeting of Protein Kinase A and Regulation of HERG Channels. Li, Yan; Sroubek, Jakub; Krishnan, ... A-kinase anchoring protein targeting of protein kinase A in the heart ...
more infohttps://www.deepdyve.com/lp/springer-journals/a-kinase-anchoring-protein-targeting-of-protein-kinase-a-and-pfrFKQvN6j
The PKA RI alpha/A-kinase anchoring proteins 10 signaling pathway and the prognosis of colorectal cancer  The PKA RI alpha/A-kinase anchoring proteins 10 signaling pathway and the prognosis of colorectal cancer
... Wang, Mojin Sichuan ... Background and AimPreviously study showed that the loss of the control of cAMP-dependent protein kinase A RI (PKA RI)/ A-kinase ... A notably higher level of AKAP10 expression was found in CRC tissues at both mRNA and protein levels. Increased expression of ... anchoring proteins 10 (AKAP10) signaling pathway initiate dysregulation of cellular healthy physiology leading to tumorigenesis ...
more infohttp://liu.diva-portal.org/smash/record.jsf?faces-redirect=true&language=no&searchType=SIMPLE&query=&af=%5B%5D&aq=%5B%5B%5D%5D&aq2=%5B%5B%5D%5D&aqe=%5B%5D&pid=diva2%3A800735&noOfRows=50&sortOrder=author_sort_asc&sortOrder2=title_sort_asc&onlyFullText=false&sf=all
Protein kinase A anchoring protein 1 | definition of protein kinase A anchoring protein 1 by Medical dictionary  Protein kinase A anchoring protein 1 | definition of protein kinase A anchoring protein 1 by Medical dictionary
... protein kinase A anchoring protein 1 explanation free. What is protein kinase A anchoring protein 1? Meaning of protein kinase ... A anchoring protein 1 medical term. What does protein kinase A anchoring protein 1 mean? ... Looking for online definition of protein kinase A anchoring protein 1 in the Medical Dictionary? ... Protein kinase A anchoring protein 1 , definition of protein kinase A anchoring protein 1 by Medical dictionary https://medical ...
more infohttps://medical-dictionary.thefreedictionary.com/protein+kinase+A+anchoring+protein+1
The DUF1669 domain of FAM83 family proteins anchor casein kinase 1 isoforms | Science Signaling  The DUF1669 domain of FAM83 family proteins anchor casein kinase 1 isoforms | Science Signaling
Two scaffold proteins, the centrosomal and Golgi N-kinase anchoring protein (CG-NAP; also known as AKAP450) and the DEAD-box ... Centrosomal anchoring of the protein kinase CK1delta mediated by attachment to the large, coiled-coil scaffolding protein CG- ... Because CK1 isoforms are thought to be constitutively active protein kinases, the ability of FAM83 proteins to anchor CK1 ... The FAM83 proteins anchor various isoforms of the constitutively active kinase CK1 to specific subcellular locations. ...
more infohttps://stke.sciencemag.org/content/11/531/eaao2341
akap13 Protein, A kinase (PRKA) anchor protein 13 - Creative BioMart  akap13 Protein, A kinase (PRKA) anchor protein 13 - Creative BioMart
... which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to ... The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins, ... Therefore, these isoforms function as scaffolding proteins to coordinate a Rho signaling pathway and, in addition, function as ... The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, ...
more infohttps://www.creativebiomart.net/symbolsearch_AKAP13.htm
WikiGenes - AKAP9 - A kinase (PRKA) anchor protein 9  WikiGenes - AKAP9 - A kinase (PRKA) anchor protein 9
Synonyms: A-kinase anchor protein 350 kDa, A-kinase anchor protein 450 kDa, A-kinase anchor protein 9, AKAP 120-like protein, ... AKAP350, a multiply spliced protein kinase A-anchoring protein associated with centrosomes. Schmidt, P.H., Dransfield, D.T., ... Association of immature hypophosphorylated protein kinase cepsilon with an anchoring protein CG-NAP. Takahashi, M., Mukai, H., ... Cloning and characterization of a cDNA encoding an A-kinase anchoring protein located in the centrosome, AKAP450. Witczak, O., ...
more infohttps://www.wikigenes.org/e/gene/e/10142.html
Glycogen synthase kinase 3beta interaction protein functions as an A-kinase anchoring protein  - Publikationsserver der...  Glycogen synthase kinase 3beta interaction protein functions as an A-kinase anchoring protein - Publikationsserver der...
A-kinase anchoring proteins (AKAPs) include a family of scaffolding proteins that target protein kinase A (PKA) and other ... A-kinase anchoring proteins (AKAPs) include a family of scaffolding proteins that target protein kinase A (PKA) and other ... Glycogen synthase kinase 3beta interaction protein functions as an A-kinase anchoring protein ... Glycogen synthase kinase 3beta interaction protein functions as an A-kinase anchoring protein. The Journal of biological ...
more infohttps://epub.uni-regensburg.de/30633/
Association of sperm protein 17 with A-kinase anchoring protein 3 in flagella | Reproductive Biology and Endocrinology | Full...  Association of sperm protein 17 with A-kinase anchoring protein 3 in flagella | Reproductive Biology and Endocrinology | Full...
This study demonstrates that Sp17 from spermatozoa binds A-kinase anchoring protein 3 (AKAP3), confirming the functionality of ... of protein kinase A (PKA); 2) a central sulphated carbohydrate-binding domain; and 3) a C-terminal Ca++/calmodulin (CaM) ... Western blots of bound and eluted proteins probed with anti-Sp17 revealed that AKAP3 bound and precipitated a significant level ... However, Sp17 protein is found predominantly in spermatozoa, cilia and human neoplastic cell lines. ...
more infohttps://rbej.biomedcentral.com/articles/10.1186/1477-7827-2-57
Akap8l MGI Mouse Gene Detail - MGI:1860606 - A kinase (PRKA) anchor protein 8-like  Akap8l MGI Mouse Gene Detail - MGI:1860606 - A kinase (PRKA) anchor protein 8-like
View mouse Akap8l Chr17:32321425-32350581 with: sequences, polymorphisms, proteins, references, function, expression
more infohttp://www.informatics.jax.org/marker/MGI:1860606
  • A-kinase anchor protein 4 (AKAP4) gene and protein expression was monitored by quantitative polymerase chain reaction (qPCR), reverse transcription (RT)-PCR and Western blotting in normal colon tissue lysate, normal colon epithelial cells and in colon cancer cell lines viz. (biomedcentral.com)
  • Our studies showed that AKAP4 gene and protein expression was expressed in all colon cancer cells while no expression was detectable in normal colon cells. (biomedcentral.com)
  • Here, we show by a variety of protein interaction assays that SFRS17A binds both type I and type II PKA in vitro and inside cells, demonstrating that SFRS17A is a dual-specific AKAP. (uky.edu)
  • Human colon xenograft studies showed a significant decrease in the levels of cyclins B1, D and E and cyclin dependent kinases such as CDK1, CDK2, CDK4 and CDK6. (biomedcentral.com)
  • A fundamental role for protein-protein interactions in the organization of signal transduction pathways is evident. (ovid.com)
  • Signal transduction through the cAMP pathway starts by stimulation of G-protein-coupled-receptors (GPCRs), via specific extracellular ligands leading to activation of adenylyl cyclase (AC), which converts ATP into cAMP. (frontiersin.org)
  • Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization. (wikipedia.org)
  • This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. (wikipedia.org)
  • The mitochondrial localization and the presence of RGS domains may have important implications for the function of this protein in PKA and G protein signal transduction. (wikipedia.org)
  • Chen L, Kass RS (2006) Dual roles of the A kinase-anchoring protein Yotiao in the modulation of a cardiac potassium channel: a passive adaptor versus an active regulator. (springer.com)
  • Because these kinases exhibit constitutive activity in biochemical assays, it is likely that their activity in cells is controlled by subcellular localization, interactions with inhibitory proteins, targeted degradation, or combinations of these mechanisms. (sciencemag.org)
  • This enzyme is responsible for phosphorylating other proteins and substrates, changing their activity. (wikipedia.org)
  • Recombinant AKAP3 and AKAP4 RII binding domains were synthesized as glutathione S-transferase (GST) fusion proteins immobilized on glutathione-agarose resin and added to CHAPS extracts of human spermatozoa. (biomedcentral.com)
  • Western blots of bound and eluted proteins probed with anti-Sp17 revealed that AKAP3 bound and precipitated a significant level of Sp17 while AKAP4 did not. (biomedcentral.com)
  • Velocity gradient centrifugation of solubilized porcine cardiac membrane proteins showed that several PKA-RI and PKA-RII binding proteins cosediment with ERG channels. (deepdyve.com)
  • it localises to the sarcoplasmic reticulum and nuclear membrane, where it anchors PKA. (thefreedictionary.com)
  • PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. (wikipedia.org)
  • It is specifically localized to the sarcoplasmic reticulum and nuclear membrane, and is involved in anchoring PKA to the nuclear membrane or sarcoplasmic reticulum. (wikipedia.org)
  • Most are peripheral membrane proteins attached at one end to Golgi membranes. (wikipedia.org)
  • 12 ] have recently confirmed by an alignment analysis the similarity of the N-terminal of Sp17 to several other proteins from testis containing RII alpha-like AKAP binding domains (ASP (AKAP-associated protein), FSII (fibrosheathin II), ropporin). (biomedcentral.com)
  • ZASP interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. (wikipedia.org)
  • The protein is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa. (wikipedia.org)
  • The proteins TP53BP1 (Tumor suppressor p53-binding protein 1) and its fission yeast homolog Crb2 and JMJD2A (Jumonji domain containing 2A) contain either tandem or double Tudor domains and recognize methylated histones. (wikipedia.org)
  • Stimulation of G-protein-coupled receptors leads to activation of adenylyl cyclase (AC), which converts ATP into cAMP. (frontiersin.org)