6-Phytase: An enzyme that catalyzes the conversion of myo-inositol hexakisphosphate and water to 1L-myo-inositol 1,2,3,4,5-pentakisphosphate and orthophosphate. EC 3.1.3.26.Phytic Acid: Complexing agent for removal of traces of heavy metal ions. It acts also as a hypocalcemic agent.Phosphorus, Dietary: Phosphorus used in foods or obtained from food. This element is a major intracellular component which plays an important role in many biochemical pathways relating to normal physiological functions. High concentrations of dietary phosphorus can cause nephrocalcinosis which is associated with impaired kidney function. Low concentrations of dietary phosphorus cause an increase in calcitriol in the blood and osteoporosis.Aspergillus niger: An imperfect fungus causing smut or black mold of several fruits, vegetables, etc.Phosphorus: A non-metal element that has the atomic symbol P, atomic number 15, and atomic weight 31. It is an essential element that takes part in a broad variety of biochemical reactions.Animal Feed: Foodstuff used especially for domestic and laboratory animals, or livestock.Soybeans: An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS.Digestion: The process of breakdown of food for metabolism and use by the body.Coccyx: The last bone in the VERTEBRAL COLUMN in tailless primates considered to be a vestigial tail-bone consisting of three to five fused VERTEBRAE.Animal Nutritional Physiological Phenomena: Nutritional physiology of animals.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Zea mays: A plant species of the family POACEAE. It is a tall grass grown for its EDIBLE GRAIN, corn, used as food and animal FODDER.Biological Availability: The extent to which the active ingredient of a drug dosage form becomes available at the site of drug action or in a biological medium believed to reflect accessibility to a site of action.Dietary Supplements: Products in capsule, tablet or liquid form that provide dietary ingredients, and that are intended to be taken by mouth to increase the intake of nutrients. Dietary supplements can include macronutrients, such as proteins, carbohydrates, and fats; and/or MICRONUTRIENTS, such as VITAMINS; MINERALS; and PHYTOCHEMICALS.Weaning: Permanent deprivation of breast milk and commencement of nourishment with other food. (From Stedman, 25th ed)Diet: Regular course of eating and drinking adopted by a person or animal.Minerals: Native, inorganic or fossilized organic substances having a definite chemical composition and formed by inorganic reactions. They may occur as individual crystals or may be disseminated in some other mineral or rock. (Grant & Hackh's Chemical Dictionary, 5th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Random Allocation: A process involving chance used in therapeutic trials or other research endeavor for allocating experimental subjects, human or animal, between treatment and control groups, or among treatment groups. It may also apply to experiments on inanimate objects.Neoprene: An oil-resistant synthetic rubber made by the polymerization of chloroprene.European Union: The collective designation of three organizations with common membership: the European Economic Community (Common Market), the European Coal and Steel Community, and the European Atomic Energy Community (Euratom). It was known as the European Community until 1994. It is primarily an economic union with the principal objectives of free movement of goods, capital, and labor. Professional services, social, medical and paramedical, are subsumed under labor. The constituent countries are Austria, Belgium, Denmark, Finland, France, Germany, Greece, Ireland, Italy, Luxembourg, Netherlands, Portugal, Spain, Sweden, and the United Kingdom. (The World Almanac and Book of Facts 1997, p842)Food Safety: Activities involved in ensuring the safety of FOOD including avoidance of bacterial and other contamination.Endo-1,4-beta Xylanases: Enzymes which catalyze the endohydrolysis of 1,4-beta-D-xylosidic linkages in XYLANS.Journal Impact Factor: A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.Bibliometrics: The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)Publishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.Endo-1,3(4)-beta-Glucanase: An endocellulase with specificity for the hydrolysis of 1,3- or 1,4-linkages in beta-D-glucans. This enzyme specifically acts on sites where reducing glucose residues are substituted at the 3 position.Commerce: The interchange of goods or commodities, especially on a large scale, between different countries or between populations within the same country. It includes trade (the buying, selling, or exchanging of commodities, whether wholesale or retail) and business (the purchase and sale of goods to make a profit). (From Random House Unabridged Dictionary, 2d ed, p411, p2005 & p283)DNA, Recombinant: Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.Citric Acid: A key intermediate in metabolism. It is an acid compound found in citrus fruits. The salts of citric acid (citrates) can be used as anticoagulants due to their calcium chelating ability.Animals, ZooEuropeAnimal Testing Alternatives: Procedures, such as TISSUE CULTURE TECHNIQUES; mathematical models; etc., when used or advocated for use in place of the use of animals in research or diagnostic laboratories.International Cooperation: The interaction of persons or groups of persons representing various nations in the pursuit of a common goal or interest.ColombiaLatin America: The geographic area of Latin America in general and when the specific country or countries are not indicated. It usually includes Central America, South America, Mexico, and the islands of the Caribbean.Travel: Aspects of health and disease related to travel.China: A country spanning from central Asia to the Pacific Ocean.Eye: The organ of sight constituting a pair of globular organs made up of a three-layered roughly spherical structure specialized for receiving and responding to light.Guinea Pigs: A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.South AmericaIntellectual Property: Property, such as patents, trademarks, and copyright, that results from creative effort. The Patent and Copyright Clause (Art. 1, Sec. 8, cl. 8) of the United States Constitution provides for promoting the progress of science and useful arts by securing for limited times to authors and inventors, the exclusive right to their respective writings and discoveries. (From Black's Law Dictionary, 5th ed, p1014)Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Information Services: Organized services to provide information on any questions an individual might have using databases and other sources. (From Random House Unabridged Dictionary, 2d ed)Genetic Research: Research into the cause, transmission, amelioration, elimination, or enhancement of inherited disorders and traits.Poultry: Domesticated birds raised for food. It typically includes CHICKENS; TURKEYS, DUCKS; GEESE; and others.Legislation, Medical: Laws and regulations, pertaining to the field of medicine, proposed for enactment or enacted by a legislative body.Sugar Alcohols: Polyhydric alcohols having no more than one hydroxy group attached to each carbon atom. They are formed by the reduction of the carbonyl group of a sugar to a hydroxyl group.(From Dorland, 28th ed)Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Access to Information: Individual's rights to obtain and use information collected or generated by others.Enterobacter aerogenes: Gram-negative, capsulated, gas-producing rods found widely in nature. Both motile and non-motile strains exist. The species is closely related to KLEBSIELLA PNEUMONIAE and is frequently associated with nosocomial infections

Expression of an Aspergillus niger phytase gene (phyA) in Saccharomyces cerevisiae. (1/247)

Phytase improves the bioavailability of phytate phosphorus in plant foods to humans and animals and reduces phosphorus pollution of animal waste. Our objectives were to express an Aspergillus niger phytase gene (phyA) in Saccharomyces cerevisiae and to determine the effects of glycosylation on the phytase's activity and thermostability. A 1.4-kb DNA fragment containing the coding region of the phyA gene was inserted into the expression vector pYES2 and was expressed in S. cerevisiae as an active, extracellular phytase. The yield of total extracellular phytase activity was affected by the signal peptide and the medium composition. The expressed phytase had two pH optima (2 to 2.5 and 5 to 5.5) and a temperature optimum between 55 and 60 degrees C, and it cross-reacted with a rabbit polyclonal antibody against the wild-type enzyme. Due to the heavy glycosylation, the expressed phytase had a molecular size of approximately 120 kDa and appeared to be more thermostable than the commercial enzyme. Deglycosylation of the phytase resulted in losses of 9% of its activity and 40% of its thermostability. The recombinant phytase was effective in hydrolyzing phytate phosphorus from corn or soybean meal in vitro. In conclusion, the phyA gene was expressed as an active, extracellular phytase in S. cerevisiae, and its thermostability was affected by glycosylation.  (+info)

Phytase improves iron bioavailability for hemoglobin synthesis in young pigs. (2/247)

Dietary phytase supplementation improves bioavailabilities of phytate-bound minerals such as P, Ca, and Zn to pigs, but its effect on Fe utilization is not clear. The efficacy of phytase in releasing phytate-bound Fe and P from soybean meal in vitro and in improving dietary Fe bioavailability for hemoglobin repletion in young, anemic pigs was examined. In Exp. 1, soybean meal was incubated at 37 degrees C for 4 h with either 0, 400, 800, or 1,200 units (U) of phytase/kg, and the released Fe and P concentrations were determined. In Exp. 2, 12 anemic, 21-d-old pigs were fed either a strict vegetarian, high-phytate (1.34%) basal diet alone, or the diet supplemented with 50 mg Fe/kg diet (ferrous sulfate) or phytase at 1,200 U/kg diet (Natuphos, BASF, Mt. Olive, NJ) for 4 wk. In Exp. 3, 20 anemic, 28-d-old pigs were fed either a basal diet with a moderately high phytate concentration (1.18%) and some animal protein or the diet supplemented with 70 mg Fe/kg diet, or with one of two types of phytase (Natuphos or a new phytase developed in our laboratory, 1,200 U/kg diet) for 5 wk. In Exp. 2 and 3, diets supplemented with phytase contained no inorganic P. In Exp. 1, free P concentrations in the supernatant increased in a phytase dose-dependent fashion (P<.05), whereas free Fe concentrations only increased at the dose of 1,200 U/kg (P<.10). In Exp. 2 and 3, dietary phytase increased hemoglobin concentrations and packed cell volumes over the unsupplemented group; these two measures, including growth performance, were not significantly different than those obtained with dietary supplemental Fe. In conclusion, both sources of phytase effectively degraded phytate in corn-soy diets and subsequently released phytate-bound Fe from the diets for hemoglobin repletion in young, anemic pigs.  (+info)

Occupational IgE sensitisation to phytase, a phosphatase derived from Aspergillus niger. (3/247)

OBJECTIVE: Phytase is a phosphatase derived from Aspergillus niger that enhances phosphate bioavailability in the gut, and therefore has been increasingly used as an animal feed additive since the early 1990s. The aim of this study was to assess whether work related respiratory symptoms among workers in a so called premix factory producing animal feed additives, could be due to type I (mediated by immunoglobulin E (IgE) allergic sensitisation to phytase. METHODS: Preparations of specific IgE against phytase as used in the factory were assessed by enzyme immunoassay (EIA) in serum samples of 11 exposed workers who regularly handled the enzyme, in 11 office and laboratory workers of the same plant (non-exposed internal controls), and in 19 laboratory animal workers as external controls. The factory workers also completed a questionnaire on common and work related respiratory symptoms. RESULTS: Depending on the cut off level in the EIA for IgE, and the preparation used as coated allergen, antiphytase sensitisation was found in one to four of the 19 external controls, in one to five of the 11 internal controls, and in four to 10 of the 11 exposed workers. Strongest IgE reactions were found in four exposed workers who reported work related respiratory symptoms, particularly wheezing, and in one internal control who possibly had become sensitised because the structure of the factory building did not preclude airborne exposure in the offices and corridors of the plant. Experiments with inhibition EIA for IgE showed that (a) phytase of another commercial source was only partially cross reactive with phytase as used in the premix factory, and (b) phytase used as an animal feed additive did not cross react with common mould extracts, except for extracts from the species of origin, Aspergillus niger. The amount of IgE binding phytase in Aspergillus niger was estimated to be between 0.1% and 1% of the extractable mould proteins. CONCLUSIONS: Phytase is a potentially important new occupational allergen causing specific IgE immune responses among exposed workers. Such IgE sensitisation could probably be the cause of work related asthmatic and other respiratory symptoms if no effective measures are taken to prevent airborne occupational exposure at sites where phytase is handled, particularly during addition of enzyme preparations to animal feed.  (+info)

Dephosphorylation of phytate by using the Aspergillus niger phytase with a high affinity for phytate. (4/247)

A phytase (EC 3.1.3.8) with a high affinity for phytic acid was found in Aspergillus niger SK-57 and purified to homogeneity in four steps by using ion-exchange chromatography (two types), gel filtration, and chromatofocusing. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified enzyme gave a single stained band at a molecular mass of approximately 60 kDa. The Michaelis constant of the enzyme for phytic acid (18.7 +/- 4.6 microM) was statistically analyzed. In regard to the orthophosphate released from phytic acid, a significant difference between a low K(m) phytase from A. niger SK-57 and a high K(m) phytase from Aspergillus ficuum was recognized.  (+info)

Comparative enzymatic hydrolysis of phytate in various animal feedstuff with two different phytases. (5/247)

Bacillus amyloliquefaciens DS11 phytase (DS11 phytase) and Aspergillus ficuum phytase (AF phytase) activities were investigated by measuring the release of phosphate from phytate in animal feedstuff such as wheat bran, corn meal, soybean meal and rice flour at pH 5 and 7. In all the tested feedstuff, the enzymatic activity of DS11 phytase was more active at pH 7, but that of AF phytase was more active at pH 5. From these results, the phytate in the gastrointestinal tract could be degraded in the small intestine or stomach by DS11 or AF phytase, respectively. In conclusion, the results presented in this paper indicated that different combination ratios of DS11 and AF phytase, depending on the kind of feedstuff, might effectively induce more enzymatic activity both in the stomach and small intestine in terms of the pH of the gastrointestinal tract.  (+info)

From DNA sequence to improved functionality: using protein sequence comparisons to rapidly design a thermostable consensus phytase. (6/247)

Naturally-occurring phytases having the required level of thermostability for application in animal feeding have not been found in nature thus far. We decided to de novo construct consensus phytases using primary protein sequence comparisons. A consensus enzyme based on 13 fungal phytase sequences had normal catalytic properties, but showed an unexpected 15-22 degrees C increase in unfolding temperature compared with each of its parents. As a first step towards understanding the molecular basis of increased heat resistance, the crystal structure of consensus phytase was determined and compared with that of Aspergillus niger phytase. Aspergillus niger phytase unfolds at much lower temperatures. In most cases, consensus residues were indeed expected, based on comparisons of both three-dimensional structures, to contribute more to phytase stabilization than non-consensus amino acids. For some consensus amino acids, predicted by structural comparisons to destabilize the protein, mutational analysis was performed. Interestingly, these consensus residues in fact increased the unfolding temperature of the consensus phytase. In summary, for fungal phytases apparently an unexpected direct link between protein sequence conservation and protein stability exists.  (+info)

Efficacy of yeast phytase in improving phosphorus bioavailability in a corn-soybean meal-based diet for growing pigs. (7/247)

Crossbred barrows (n = 66; 6 wk old) were used in a 6-wk experiment to evaluate the efficacy of phytase from yeast or Aspergillus niger on performance, tibial characteristics, and serum inorganic P concentration. We also investigated the stability of these phytases in acidic solutions with pepsin, which simulated gastric conditions. Pigs were fed a P-adequate diet containing .34% nonphytate-P or a low-P diet containing .20% nonphytate-P. The low-P diet was supplemented with 0, 1,000, 2,000, or 4,000 phytase units (PU; the activity at optimal pH, i.e., pH 4.2 for yeast phytase and pH 5.5 for phytase from Aspergillus niger)/kg of yeast phytase, or 1,000 PU/kg phytase from Aspergillus niger. The graded level of yeast phytase linearly increased ADG (P = .047), tibial weight (P = .091), tibial density (P < .001), and P concentration in tibial cortex (P = .018). Aspergillus niger phytase also increased ADG (P = .022), serum inorganic P concentration (P < .001), tibial density (P = .007), and tibial P concentration (P = .025). The pigs given 1,000 PU/kg Aspergillus niger phytase showed greater ADG (P = .091), tibial density (P= .001), and tibial P concentration (P = .062) than those given 1,000 PU/kg yeast phytase. No measurements differed (P > .31) between the pigs given 1,000 PU/kg Aspergillus niger phytase and those given 4,000 PU/kg yeast phytase. These results suggested that yeast phytase improves bioavailability of P in the diet for growing pigs but the efficacy of yeast phytase is less than that of Aspergillus niger phytase. During incubation in acidic solutions with pepsin, yeast phytase (P < .001) lost more of its activity than Aspergillus niger phytase. This lesser stability of yeast phytase may be responsible for the poorer efficacy of yeast phytase than that of Aspergillus niger. In summary, supplementation of swine diets with yeast phytase is beneficial, but its efficacy is less than that of Aspergillus niger phytase.  (+info)

Effects of dietary calcium:phosphorus ratios on apparent absorption of calcium and phosphorus in the small intestine, cecum, and colon of pigs. (8/247)

Thirty-two crossbred barrows were used to investigate the effects of dietary Ca:total P (tP) ratios in phytase-supplemented diets on the apparent absorption of P and Ca in the small intestine, cecum, and colon. Three Ca:tP ratio treatments (1.5:1, 1.3:1, or 1.0:1) were created by adjusting the amount of ground limestone added to the basal low-P grower (.39% tP including .07% added inorganic P) and finisher (.32% tP without added inorganic P) diets. All low-P ratio diets were supplemented with Natuphos phytase at 500 units/kg. A positive control diet without phytase supplementation contained adequate P and Ca to meet dietary requirements. At 123 kg, the pigs were slaughtered and the contents of ileum, cecum, and colon were collected. Lowering the dietary Ca:tP ratio in the diets containing phytase linearly increased (P < .01) the apparent absorption (% and g/d) of P in the small intestine, but Ca absorption was not affected. Pigs fed the low-P diet with a Ca:tP ratio of 1.0:1 had an apparent absorption (g/d) of P or Ca similar to that of pigs fed the control diet, which was adequate in Ca and P. Averaged across all diets, the apparent absorption of P was highest when measured at the cecum, and the apparent absorption of Ca was highest when measured at the colon. In conclusion, lowering the dietary Ca:tP ratio to 1.0:1 in a low-P diet containing phytase increased the apparent absorption of P in the small intestine. Furthermore, a significant amount of P was absorbed in the cecum.  (+info)

Three barrows weighing 45.0 kg, fitted with simple T-cannulas in both the duodenum and terminal ileum, were assigned to diets in a 3횞3 Latin Square design experiment to determine the effects of two calcium levels (0.8% vs 0.4%) on phytase activity and nutrient balance in growing pigs. The control diet contained 0.8% calcium, with no added inorganic phosphorus (0.45% total phosphorus) and no added phytase. The two additional experimental diets contained microbial phytase (750 phytase units/kg) and supplied either 0.8% or 0.4% calcium. With added microbial phytase, ileal and total tract digestibility of total phosphorus were improved by 20.9 and 13.8 percentage units, respectively (p=0.01). The apparent duodenal and ileal digestibility of phytate phosphorus were increased by 51.8 and 49.7 percentage units. (p=0.001). Lowering dietary calcium in the presence of microbial phytase increased the digestibility of phytate phosphorus by an additional 10.9 (p=0.001) and 5.7 percentage units for duodenal ...
Phytate is considered as one of the antinutritional factors exist mainly in different plants; it chelates many nutrition factors and denaturing many proteins. Recent research dealed with the isolation of extracellular phytase producing Bacillus species from local soil samples; the effect of phytase on different sources of phytate. Twenty five bacterial isolates of Bacillus species produced extracellular phytase on pikovaskaya agar or phytase screening medium( PSM). The clear zone around bacterial growth indicated for enzyme production. Soil samples were collected from many agricultural region includes Baghdad, Karballa, Babylon, Basrah, Emarah and al- Sulaimania. Five bacterial isolates had the ability to digest phytate extracted from different sources such as wheat bran, barly, rice bran, chick bea, mung bean, grass bea and phasolias. Other bacterial isolates could digest some of phytate sources. Extracellular phytase activity were determined at different pHs buffer for five chosen isolates using
Weaned crossbred barrows (n=126) were used to investigate the effect of four different levels of a new Zymetrics Inc. phytase (100 Units (U); 500 U; 2,500 U; or 12,500 U) on low phosphorus corn-soybean meal diets. This tested the theory that supplementation of higher phytase amounts to a low phosphorus diet will increase bone strength as well as maintain growth performance at a higher rate than those of lower or no phytase supplementation. Pigs were adjusted to a common Phase I diet for two weeks post-weaning before allocation to 1 of 6 dietary treatments for 4 weeks. Increasing supplemental phytase showed a linear increase in ADG (p , .01) and also showed an linear increase in overall body weight (BW) during the growing and finishing stages of the experiment. Daily feed intake and feed efficiency showed a linear increase (p , .01) and overall breaking strength increased linearly with phytase supplementation. Gain to feed ratios in pigs with 2,500 U and 12,500 U showed better performance than ...
Research on the effect of addition of phytase enzymes in artificial feed on Barramundi (Lates calcarifer, Bloch) feed efficiency and growth, this research was held at BPBL Batam. The purpose of this study was to determined dosage of phytase enzymes to be added to medium protein feed (PF) to efficiently feed and increase the growth of Barramundi. The methode used in this study was RAL, using 4 treatment and 3 replications. The treatments used in this study were: A) 0 g phytase enzymes, B) 0,5 g phytase enzymes, C) 1 g phyase enzymes, D) 1,5 g phytase enzymes per kg of PF artificial feed. The results of feed efficiency and daily weight growth of this study showed that treatment D was the best results 20,41 % and 5,1 % respectivly.. ...
The use of genetically engineered probiotics to express specific enzymes has been the subject of considerable attention in poultry industry because of increased nutrient availability and reduced cost of enzyme supplementation. Phytase enzyme is commonly added to poultry feed to improve digestibility and availability of phosphorus from plant sources. To construct a probiotic with potential of phytate degradation, phytase gene (appA) from E. coli was cloned and transformed into two probiotic bacteria Lactobacillus salivarius and Lactococcus lactis. The results showed plasmid instability, unable to express the gene. The expression of appA gene in L. lactis was analyzed by detecting specific RNA and zymography assay. Phytase enzyme was isolated from cellular extracts of recombinant L. lactis, showing a 46 kDa band upon the SDS-PAGE analysis. Zymogram also confirmed the phytase activity of the 46 kDa band corresponding to the enzyme. An enzyme activity of 4.9 U mL-1 was obtained in cell extracts of L. lactis
Recently, we engineered Pichia pastoris Muts strains to produce several beta-propeller phytases, one from Bacillus subtilis and the others designed by a structure-guided consensus approach. Furthermore, we demonstrated the ability of P. pastoris to produce and secrete these phytases in an active form in shake-flask cultures. In the present work, we used a design of experiments strategy (Simplex optimization method) to optimize five environmental factors that define the culture conditions in the induction step to increase beta-propeller phytase production in P. pastoris bioreactor cultures. With the optimization process, up to 347,682 U (82,814 U/L or 6.4 g/L culture medium) of phytase at 68 h of induction was achieved. In addition, the impact of the optimization process on the physiological response of the host was evaluated. The results indicate that the increase in extracellular phytase production through the optimization process was correlated with an increase in metabolic activity of P. ...
China sell poultry feed supplement( phytase micro granule) with High-Quality, Leading sell poultry feed supplement( phytase micro granule) Manufacturers & Suppliers, find sell poultry feed supplement( phytase micro granule) Factory & Exporters.
China Phytase mixer granulator dryer machine equipment with High-Quality, Leading Phytase mixer granulator dryer machine equipment Manufacturers & Suppliers, find Phytase mixer granulator dryer machine equipment Factory & Exporters.
Phytase supplementation in broiler diets is a common practice to improve phosphorus (P) availability and to reduce P loss by excretion. An enhanced P availability, and its concomitant supplementation with calcium (Ca), can affect the structure of the microbial community in the digestive tract of broiler chickens. Here, we aim to distinguish the effects of mineral P, Ca, and phytase on the composition of microbial communities present in the content and the mucosa layer of the gastrointestinal tract (GIT) of broiler chickens. Significant differences were observed between digesta and mucosa samples for the GIT sections studied (p= 0.001). The analyses of 56 individual birds showed a high microbial composition variability within the replicates of the same diet. The average similarity within replicates of digesta and mucosa samples across all diets ranged from 29 to 82% in crop, 19-49% in ileum, and 17-39% in caeca. Broilers fed with a diet only supplemented with Ca had the lowest body weight gain ...
The event, themed The Value Chain of Phytate Destruction, has seen feed and nutrition experts from around the world share the latest research into how combatting phytate can improve feed efficiency, nutrient utilisation, sustainability and ultimately profitability.. On the final day, Professor Hank Classen, University of Saskatchewan, highlighted the impact of phytate and phytate esters on protein digestibility and the influence of superdoses of phytase on amino acid digestibility, specifically non-essential amino acids.. "The use of high levels of phytase is promising in young birds because we did see a more consistent response in phytate hydrolysis and so the availability of amino acids and energy is also more predictable.". Professor Layi Adeola of Purdue University discussed the fate of amino acids in the gastrointestinal tract, a very complex system of endogenous enzymes and transporters.. This research was followed by that of Dr Sami Dridi, University of Arkansas, and Dr Gabriel Morales, ...
The latest product in DuPonts nutribiotic approach to feed strategy, Axtra® PHY GOLD offers superior performance and higher feed cost savings compared to other phytases in the marke.. DuPont Nutrition & Biosciences announced today the launch of the industry-leading novel phytase enzyme, Axtra® PHY GOLD. The enzyme offers superior performance compared to other phytases on the market, delivering greater feed cost savings to producers. Axtra® PHY GOLD will be available in the United States, Mexico and India and eventually worldwide, pending regulatory authorizations.. Recent scientific studies demonstrate that Axtra® PHY GOLDs ability to allow formulation of inorganic phosphate-free high phytate diets, improves sustainability of animal production. It also offers market-leading thermostability, proven to outperform other commercial phytases under a wide range of pelleting conditions, making it the natural choice for applications where pelleting performance is a primary concern.. Phytate is the ...
The patients medical history, bibasal lung crackles at auscultation, the restrictive lung function pattern with decreased diffusion capacity for carbon monoxide, oxygen tension decrease on exertion, radiological features, increased specific IgG to phytase, and the positive exposure test (including the BAL results), all support the diagnosis of hypersensitivity pneumonitis (HP) 5-7. Furthermore, the clinical improvement after treatment and cessation of the contact to phytase strongly suggest HP due to inhalation of phytase. To the best of our knowledge, this has never been reported before.. Phytase is an enzyme that catalyses the hydrolysis of phytate, a phosphate-storage mode present in cereals and soy, to inorganic phosphate. Monogastric animals (pigs and poultry) can only partially utilise this form of phosphorus. To increase the bioavailability of phosphorus for these types of animals, fungal phytase is added to the feed. This results in a decreased need to add inorganic phosphorus and a ...
Bacteria secreted enzyme mostly are cell associated and the only bacteria that produces extracellular phytases are of the genera Bacillus (Choi et al., 2001; Kerovou et al., 1998; Kim et al., 1998). Bacterial phytases are detected in many species like Bacillus, Pseudomonas, E.coli, Klebsiella, Lactobacillus species (Quan et al., 2001; Pandey et al., 2001; Kim et al., 1999, 1998; Griener et al.,1993; Shimuzu, 1992; Yoon et al.,1996). Bacterial phytases are generally produced by submerged fermentation at pH 7.0. Some bacterial phytases, especially those of the genera Bacillus and Enterobacter, exhibit pH optima in the range from 6.0 to 8.0 (Shimizu, 1992). Therefore, they would be more beneficial as feed additives for poultry as their pH optimum is close to the physiological pH of the poultry crop. The phytases of E. coli have been reported to be periplasmic enzymes (Greiner et al., 1993) and phytase activity in Selenomonas ruminantium, Bacillus spp. and Mitsuokella multiacidus was found to be ...
In the present study, we utilized TALEN- and CRISPR/Cas9-induced mutations to analyze the promoter of the barley phytase gene HvPAPhy_a. The purpose of the study was dual, validation of the PAPhy_a...
Bio Blend® consists of a synergistic consortium of targeted beneficial microorganisms, minerals, plants and animal by-products that are combined to produce a fermented feed grade product to supplement the animal diet. The production of quality meat and milk begins with healthy animals. Modern animal feeds are predominately composed of plant material, mainly cereals and vegetable proteins. Much of these ingredients cannot be fully digested and utilized, especially by monogastric animals. Enzymes increase the digestibility of these modern animal feeds, which improve feed to gain ratios for ruminants and monogastric animals alike.. Enzymes can provide more energy due to better utilization of nutrients which promotes healthy weight gain, enhances immune response, improves skin and coat condition, and increases and maintains vitality for ruminant and monogastric animals alike.. The balance of beneficial and potentially harmful bacterial in the animal gut is an important aspect in the overall health ...
Phosphate is essential for optimal growth of swine and poultry. Because pigs and poultry are unable to metabolize this nutrient directly from plant sources, phosphorus supplements are often added to animal feed. This increases the phosphorus content of manure, which when spread over fields tends to seep into the ground water and pollute lakes and streams with drastic environmental consequences.. A less common approach taken by some farmers has been to add the enzyme phytase directly to the feed, which releases the phosphorus compounds from plant matter. While eliminating the need for supplements, phytase-treated food is expensive to produce and the enzyme can lose its potency during storage. Cecil Forsberg, of the University of Guelph, in Ontario, and colleagues believe that engineering animals with the phytase gene incorporated into their genome overcomes these issues. Forsbergs team engineered mice that secrete the bacterial enzyme phytase in their saliva. The enzyme allows the animals to ...
There are many types of feed additives on the market today that perform a variety of specific functions. Certain additives promote optimal growth and others improve palatability. Phytase is a feed additive that breaks down phytates to promote the release of inorganic phosphorus bound in plant-based feed ingredients. Phytate is classified as an indigestible acid…
An experiment was conducted to investigate the effect of phytase transgenic corn (PTC) on intestinal microflora, and the fate of transgenic DNA and protein in the digesta and tissues of broilers. A total of 160 1-day-old Arbor Acres commercial male broilers were randomly assigned to 20 cages (8 chicks per cage) with 10 cages (replicates) for each treatment. Birds were fed with a diet containing either PTC (54.0% during 1-21 days and 61.0% during 22-42 days) or non-transgenic isogenic control corn (CC) for a duration of 42 days. There were no significant differences (P|0.05) between birds fed with the PTC diets and those fed with the CC diets in the quantities of aerobic bacteria, anaerobic bacteria, colibacillus and lactobacilli, or microbial diversities in the contents of ileum and cecum. Transgenic phyA2 DNA was not detected, but phyA2 protein was detected in the digesta of duodenum and jejunum of broilers fed with the PTC diets. Both transgenic phyA2 DNA and protein fragments were not found in the
I see that this was obtained from a pre-existing library that they made, but the relative paper (reference 36) is not yet available. So, we do not know what the number of clones in the library was, what was the insert size and what was the vector. These did not appear in the Results or in the Methods section. I also thought that they could have described, very briefly, how they screened the library for the activity in the E. coiicolonies.. Rebuttal:. The required information (number of clones of the library, vector, insert size and a brief description of the screening strategy) was added to the methods section. (Lines 387-393).. They then describe the make-up of the particular cloned insert and find many open reading frames. What seemed very odd was that there were predicted ORFs on both stands for nearly the whole insert. They do not make comments on this, though. I think that they should.. Rebuttal:. The corresponding explanation was added in the methods section (lines 397-400).. I also think ...
According to the latest research, up to 30% of the performance response seen in broilers following phytase superdosing may be due to the beneficial effects of the inositol released following complete phytate degradation.
Good performance of p16/ki-67 dual-stained cytology for surveillance of women treated for high-grade CIN. Possible explanation of the basic human capacity for emotional experience Dephosphorylation of phytate by levitra vs viagra using the Aspergillus niger phytase with a high affinity for phytate. Identification of viagra substitute DNA-reactive B cells in patients with systemic lupus erythematosus. Contribution of exogenous genetic elements to the group A Streptococcus metagenome.. The FA-CS nanoparticles (NPs) were prepared by crosslinking FA-CS conjugates with sodium tripolyphosphate (STPP). The differentiation level could be sildenafil citrate estimated by two-dimensional cell morphology analysis using a conventional phase-contrast microscope. The observed preferences cannot be explained by any single structural principle, but point instead to the interaction of several principles. Catechols are co-planar cis-diols known to form female viagra pills stable, isolable complexes with borate ...
Fulltext - The Effect of Phytase and Saccharomyces cervisiae (Sc47) Supplementation on Performance, Serum Parameters, Phosphorous and Calcium Retention of Broiler Chickens
Gone are the days when phytase was only used to unlock phosphorus. Superdosing phytase - at a higher dosage than conventional dosing - has been shown to lead to so-called extra-phosphoric effects. The result is higher performance gains in pig production.
Citation: Mullaney, E., Sethumadhavan, K., Boone, S., Lei, X.G., Ullah, A.H.J. 2012. Elimination of a disulfide bridge in Aspergillus niger NRRL 3135 Phytase (PhyA) enhances heat tolerance and optimizes its temperature versus activity profile. Advances in Biological Chemistry. 2(4):372-378. Interpretive Summary: Today, much of the animal feed produced for swine, poultry and other animals with simple stomachs contain grains and other plant material rich in phytate. Phytate is common in plants and is high in phosphorus, which is an essential nutrient. However these animals lack an enzyme in their digestive tract, a phytase, that would allow them to break the phytate down and to access the phosphorus bound up in phytate. To overcome this problem, a phytase from a mold is produced and marketed as a feed additive. It allows the animals to use the phytin phosphorus and also as a bonus lowers the phosphorus levels in their manure to protect the environment. The enzyme has been widely accepted and ...
BASF Management Consulting: the internal management consultancy of the BASF Group.. BASF Management Consulting is the internal management consultancy of the BASF Group located in Ludwigshafen, Monheim (near Düsseldorf), Hong Kong, Singapore and Florham Park (USA). We advise our clients - the BASF top management - globally in various commercial, organizational, post-merger integration, carve-out, and change projects.. Our objective is to be the preferred and trusted partner of BASF units worldwide in general management questions.. We are a team of approximately 150 colleagues - with diverse academic, professional and cultural backgrounds - coming together to work with our clients on relevant and sustainable solutions. We maintain our independence and build credibility by taking personal responsibility for our actions. Pragmatic, structured and composed; we strike the right tone with our clients, between colleagues, over hierarchical boundaries and working areas.. ...
Were on the ground here in Atlanta for the 2014 International Production and Processing Expo (IPPE) and weve kicked off our involvement in this years show with a strong scientific foundation. Monday at the International Poultry Scientific Forum (IPSF), Dr. Juxing Chen presented an oral presentation titled "Effect of probiotics on the development of bacterial chondronecrosis with osteomyelitis in poultry" and Dr. Megharaja Manangi presented two oral presentations titled "Forty-two day evaluation of a microbial phytase in phosphorus deficient broiler diets via live broiler performance and tibia bone ash" and "Twenty-one day evaluation of microbial phytase inclusion in phosphorus deficient broiler diets via live broiler performance and tibia bone ash." Dr. Shelly McKees (Auburn University) presentation "How Consumer Preference Drives Poultry Processing" at the Novus IPSF luncheon was another scientific highlight. An attentive crowd listened to Dr. McKee discuss current challenges along the ...
Microorganisms that release plant-available phosphate from natural soil phosphate stores may serve as biological alternatives to costly and environmentally damaging phosphate fertilizers. To explore this possibility, we engineered a collection of root bacteria to release plant available orthophosphate from phytate, an abundant phosphate source in many soils. We identified 82 phylogenetically diverse phytase genes, refactored their sequences for optimal expression in Proteobacteria, then synthesized and engineered them into the genomes of three root-colonizing bacteria. Liquid culture assays revealed 41 engineered strains with high levels of phytate hydrolysis. Among these, we identified 12 strains across three bacterial hosts that confer a growth advantage on the model plant Arabidopsis thaliana when phytate is the sole phosphate source. These data demonstrate that DNA synthesis approaches can be used to generate plant-associated strains with novel phosphate solubilizing capabilities.Importance ...
BASF SE Kommunikation BASF - BASFs Human Nutrition business unit presents the innovative Crosspure® filter aid / Newly designed trade show booth to be unveiled
BASF Exits the Lysine Business…. // Chemical Week;4/4/2007, Vol. 169 Issue 12, p4 The article reports on the decision of BASF to exit the lysine business. BASF has decided to quit the lysine business less than 10 years after it entered the sector. BASF will close a 100,000-million tonne/year lysine unit at Gunsan, Korea by mid-year with the loss of about 180 jobs. Exiting... ...
Feed enzymes lead to better feed conversion by the animal, improved feed quality, and represent an active contribution to animal welfare. Feed enzymes help to break down anti-nutritional factors (fiber and phytate) that are present in many feed ingredients. They are used to increase the availability of starch, protein, amino acids, and minerals, such as phosphorus and calcium from feed ingredients. In addition, they can be used to supplement the enzymes produced by young animals where, because of an immature digestive system, enzyme production may be inadequate. Enzymes are proteins that are ultimately digested or excreted by the animal, leaving no residue in meat or eggs ...
Grain legumes can contribute to cropping systems through their ability to fix atmospheric N2in their root-nodules. However, the symbiotic nitrogen fixation process requiadditional phosphorus. Thus,...
611239DNAEscherichia coliCDS(1)..(1239) 1cag agt gag ccg gag ctg aag ctg gaa agt gtg gtg att gtc agt cgt 48Gln Ser Glu Pro Glu Leu Lys Leu Glu Ser Val Val Ile Val Ser Arg 1 5 10 15 cat ggt gtg cgt gct cca acc aag gcc acg caa ctg atg cag gat gtc 96His Gly Val Arg Ala Pro Thr Lys Ala Thr Gln Leu Met Gln Asp Val 20 25 30 acc cca gac gca tgg cca acc tgg ccg gta aaa ctg ggt tgg ctg aca 144Thr Pro Asp Ala Trp Pro Thr Trp Pro Val Lys Leu Gly Trp Leu Thr 35 40 45 ccg cgc ggt ggt gag cta atc gcc tat ctc gga cat tac caa cgc cag 192Pro Arg Gly Gly Glu Leu Ile Ala Tyr Leu Gly His Tyr Gln Arg Gln 50 55 60 cgt ctg gta gcc gac gga ttg ctg gcg aaa aag ggc tgc ccg cag tct 240Arg Leu Val Ala Asp Gly Leu Leu Ala Lys Lys Gly Cys Pro Gln Ser 65 70 75 80 ggt cag gtc gcg att att gct gat gtc gac gag cgt acc cgt aaa aca 288Gly Gln Val Ala Ile Ile Ala Asp Val Asp Glu Arg Thr Arg Lys Thr 85 90 95 ggc gaa gcc ttc gcc gcc ggg ctg gca cct gac tgt gca ata acc gta 336Gly Glu Ala Phe Ala Ala Gly Leu Ala Pro Asp Cys Ala Ile Thr ...
... , one of the most modern mills in Ireland, manufacturing high quality animal feeds for ruminant and monogastric animals (Beef, Dairy, Calf, Sheep, Horse, Pig & Poultry).
Michael Ceranski is the senior vice president, global business unit at BASF Human Nutrition and a man who is instrumental in determining the fate of BASFs new nutrition activities. He took some time to speak with us at Vitafoods Europe last week.. "What is truly important for us is to take out the customercentricity and the innovativeness of the two units and with all the synergies bring that to the next level in the market and to grow even stronger in the future," ​Ceranski relayed.. We create chemistry​. While the Cognis input saw the new Nutrition unit double its revenue to about €2bn in 2011 - it is a small part of BASFs €73.5bn income last year. But Ceranski said the division was central to the wider groups just-launched, 10-year we create chemistry strategy.. "Food and nutrition is at the core of the BASF group so the business of human nutrition is also at the core so that means that BASF wants to invest in research, development applications but also in people to make sure ...
Trostberg, home of BASF Construction Solutions GmbH, is the birthplace of Construction Chemicals and simultaneously a site of BASF, the globally leading Chemical Company. Trostberg is home to 110 employees of BASF Construction Chemicals. Further 140 are employed at sites Mannheim and Staßfurt (Saxony-Anhalt).. While the Construction Chemicals global Research and Development are bundled in Trostberg, Staßfurt is the center of production for readily formulated admixtures and additives, raw materials for admixtures, cement additives, solutions for underground construction, sealants, solutions for concrete repair and protection, grouts and high performance floors.. ...
China Basf manufacturers - Select 2020 high quality Basf products in best price from certified Chinese Transparent Blue manufacturers, Titanium Dioxide suppliers, wholesalers and factory on Made-in-China.com
BASF and Lutianhua have signed a MoU to build a pilot plant for a new process in synthetising dimethyl ether developed by BASF and Linde.
Baur X, Melching-Kollmuss S, Koops F, Strassburger K, Zober A, IgE-mediated allergy to phytase -- a new animal feed additive. Allergy. 2002 Oct;57(10):943- ...
The global shotcrete market is segmented based on various parameters such as system, technique, application, and geography. Based on system of spraying, the shotcrete market is classified into manual system and robotic spraying system. Technique-based classification of shotcrete market is Wet mix shotcrete process and Dry mix shotcrete process. Further, application based classification for shotcrete market includes Underground construction, Water retaining structures, protective coatings and repair works. Wet mix shotcrete is a widely-used process of shotcrete utilization for construction purpose, whereas Dry mix shotcrete is currently used for repair and restoration works as intermittent spraying needs are much more easily accomplished using dry mix. Key players profiled in the report include BASF SE, Heidelberg Cement, Grupo ACS, Lafargeholcim, Cemex, Sika AG, GCP Applied Technologies, U.S. Concrete, Mapei, and Normet.. ...
* Efficient alternative to conventional technologies, , , , Toyo Engineering Corporation (TOYO), Chiba/Japan, and BASF have entered into a cooperation to market and sell a gas purification technology spec...
BASF Personal Care and Nutrition - The global sun care market has grown over recent years with higher SPF products becoming increasingly important. Manufacturers...
View the product label for Secure 67 C from BASF Ag Products. See active ingredients, product application, restrictions, and more.
BASF was named the 2017 FCA US LLC Sustainability Supplier of the Year for the second time. Winners were selected based on a rating system that evaluates supplier performance in quality, delivery, cost, warranty, and par
DETROIT - BASF was named a 2018 General Motors (GM) Supplier of the Year for the fourteenth time since 2002. The award is presented to suppliers who distinguish themselves by meeting performance metrics for quality, execution, innovation and total enterprise cost.
* Extensive service and customized products, , , , * ChemSpec major fine and specialty chemicals trade show, , , , , , , , BASF will present its chemical intermediates and inorganics portfolio at the ChemSp...
... BASFs Catalysts division is the worlds leading supplier of environmental and process catalysts. Catalysts offers exceptional expertise in the development of technologies that protect the air we breathe, produce the fuels that power our world and ensure efficient production of a wide variety of chemicals, plastics and other products including advanced battery materials.
Univar Solutions offers Amine Catalysts from BASF and is your one-stop-shop for quality chemicals from world-class suppliers to meet your formulation needs. Contact today!
BASF is championing sustainability at Interpack 2017 with a series of products and innovations from its packaging portfolio in hall 10, booth 43.
BASF experts from different fields will share their latest scientific findings at this years International Federation of Societies of Cosmetic Chemists (IFSCC) Congress, which takes place
RONOZYME ® WX is an additive that contains endo‐1,4‐β‐xylanase which is authorised for use as a feed additive in poultry for fattening, weaned piglets and pigs for fattening. The Panel on additives and Products or Substances used in Animal Feed (FEEDAP) d .... ...
Kiarie, E.; Woyengo, T.; Nyachoti, C. M. (6 May 2015). "Efficacy of New 6-Phytase from Buttiauxella spp. on Growth Performance ... doi:10.1016/S0721-9571(81)80016-6. Kämpfer, Peter. "Buttiauxella". Wiley Online Library. doi:10.1002/9781118960608.gbm01139. ...
... map kinase kinase 6 MeSH D08.811.913.696.620.682.700.565.700 --- map kinase kinase 7 MeSH D08.811.913.696.620.682.700.567 --- ... map kinase kinase 6 MeSH D08.811.913.696.620.682.725.200.700 --- map kinase kinase 7 MeSH D08.811.913.696.620.682.725.300 --- ... cyclin-dependent kinase 6 MeSH D08.811.913.696.620.682.700.646.625 --- glycogen synthase kinase 3 MeSH D08.811.913.696.620.682. ... l-lysine 6-transaminase MeSH D08.811.913.477.700.700 --- ornithine-oxo-acid transaminase MeSH D08.811.913.477.700.800 --- ...
3-phytase EC 3.1.3.9: glucose-6-phosphatase EC 3.1.3.10: glucose-1-phosphatase EC 3.1.3.11: fructose-bisphosphatase EC 3.1.3.12 ... 5-phytase EC 3.1.3.73: a-ribazole phosphatase EC 3.1.3.74: pyridoxal phosphatase EC 3.1.3.75: phosphoethanolamine/ ... 4-phytase EC 3.1.3.27: phosphatidylglycerophosphatase EC 3.1.3.28: ADP-phosphoglycerate phosphatase EC 3.1.3.29: N- ... Identical to EC 3.5.1.25, N-acetylglucosamine-6-phosphate deacetylase EC 3.5.1.81: N-acyl-D-amino-acid deacylase EC 3.5.1.82: N ...
... acid phosphatase alkaline phosphatase fructose-bisphosphatase glucose-6-phosphatase phosphofructokinase-2 phosphoprotein ... phosphatase calcineurin 6-phytase phosphodiesterase phosphatase Phosphoric Monoester Hydrolases at the US National Library of ...
4-phytase (EC 3.1.3.26), and 5-phytase (EC 3.1.3.72). To date, most of the known phytases are 3-phytases or 4-phytases, only a ... such as phytases from Bacillus sp., Aspergillus sp., E. coli and those phytases belonging to the class of PTP-like phytases ... protein tyrosine phosphatase-like phytases (PTP-like phytases). Most of the known phytases belong to a class of enzyme called ... Most phytases are able to cleave five of the six phosphate groups from phytic acid. Phytases have been grouped based on the ...
Lim PE; Tate ME (1973). "The phytases. II. Properties of phytase fractions F 1 F 2 from wheat bran and the myoinositol ... In enzymology, a 4-phytase (EC 3.1.3.26) is an enzyme that catalyzes the chemical reaction myo-inositol hexakisphosphate + H2O ... Tomlinson RV; Ballou CE (1962). "Myoinositol polyphosphate intermediates in the dephosphorylation of phytic acid by phytase". ... phytase, and phytate 6-phosphatase. This enzyme participates in inositol phosphate metabolism. Johnson LF; Tate ME (1969). "The ...
Other names in common use include 1-phytase, phytase, phytate 1-phosphatase, and phytate 6-phosphatase. This enzyme ... In enzymology, a 3-phytase (EC 3.1.3.8) is an enzyme that catalyzes the chemical reaction myo-inositol hexakisphosphate + H2O ... the inositol pentaphosphate products of Aspergillus ficuum phytases". J. Bacteriol. 112 (1): 434-8. PMC 251429 . PMID 4342816. ... 6-pentakisphosphate and phosphate. This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric ...
As of late 2007, only the phytase purified from lily pollen had its structure solved, with PDB accession codes 1U24, 1U25, 1U26 ... Of the hundreds of phytase enzymes that have been characterized in the literature, only two have been characterized as 5- ... phytases. A histidine acid phosphatases purified from lily pollen and a protein tyrosine phosphatase-like phytase from ... In enzymology, a 5-phytase (EC 3.1.3.72) is an enzyme that catalyzes the chemical reaction myo-inositol hexakisphosphate + H2O ...
Phytase *Beta-propeller phytase. *Inositol-phosphate phosphatase *IMPA1, IMPA2, IMPA3. *Protein phosphatase: Protein tyrosine ... doi:10.1186/1750-1172-6-27. PMC 3118311. PMID 21599942.. *^ Jun HS, Lee YM, Song KD, Mansfield BC, Chou JY (April 2011). "G-CSF ... Glucose 6-phosphatase (EC 3.1.3.9, G6Pase) is an enzyme that hydrolyzes glucose 6-phosphate, resulting in the creation of a ... Wikimedia Commons has media related to Glucose 6-phosphatase.. *Glucose-6-Phosphatase at the US National Library of Medicine ...
Degradation of Phytate in Foods by Phytases in Fruit and Vegetable Extracts B.Q. PHILLIPPY AND C.J. WYATT Dendougui, Ferial; ... Ruminants are readily able to digest phytate because of the phytase produced by rumen microorganisms. In most commercial ... Dephytinisation with Intrinsic Wheat Phytase and Iron Fortification Significantly Increase Iron Absorption from Fonio ( ... "Phytases: attributes, catalytic mechanisms, and applications" (PDF). United States Department of Agriculture-Agricultural ...
Also expressed by this species are broad-specificity phytases that are efficient in breaking down phytic acid to be used for ... "Biochemical Characterization of Fungal Phytases (myo-Inositol Hexakisphosphate Phosphohydrolases) Catalytic Properties". Appl. ... 31 (6): 819-826. Wyss, M.; Brugger, R.; Kronenberger, A.; Remy, R.; Fimbel, R.; Oesterhelt, G.; Lehmann, M.; van Loon, A. (1999 ... 49 (8): 883-6. doi:10.3109/13693786.2011.584218. PMID 21619496. Destino, Lauren; Sutton, Deanna A; Helon, Anna L; Havens, Peter ...
... the amount of phytic acid is commonly reduced in animal feeds by adding histidine acid phosphate type of phytases to them.[5] ... 35 (6): 974. doi:10.1021/jf00078a028.. *^ Heck AM, Yanovski JA, Calis KA (March 2000). "Orlistat, a new lipase inhibitor for ... For example, Bowman-Birk trypsin inhibitor is found in soybeans.[6] Lipase inhibitors interfere with enzymes, such as human ... "In silico characterization of histidine Acid phytase sequences". Enzyme Research. 2012: 845465. doi:10.1155/2012/845465. PMC ...
For example, it can produce a high yield of phytase, an important industrial enzyme. It also has an increased extracellular ... Bogar, B.; Szakacs, G.; Pandey, A.; Abdulhameed, S.; Linden, J.C.; Tengerdy, R.P. (4 April 2003). "Production of Phytase by ... 16 (6): 397-402. PMID 3242377. Zhao, Z; Li, L; Wan, Z; Chen, W; Liu, H; Li, R (April 2011). "Simultaneous detection and ... 40 (3): 181-6. doi:10.1111/j.1398-9995.1985.tb00214.x. PMID 4039540. Güneser, S; Atici, A; Köksal, F; Yaman, A (1994). "Mold ...
"A novel beta-propeller phytase from Pedobacter nyackensis MJ11 CGMCC 2503 with potential as an aquatic feed additive". Applied ... 59 (Pt 7): 1720-6. doi:10.1099/ijs.0.000158-0. PMID 19542109. Huang, Huoqing; Shao, Na; Wang, Yaru; Luo, Huiying; Yang, Peilong ...
Pileggi VJ, De Luca HF, Steenbock H (September 1955). "The role of vitamin D and intestinal phytase in the prevention of ... 64 (6): 871-877. Mithal, A.; Wahl D.A., Bonjour J.P., Burckhardt T., Dawson-Hughes B., Eisman J.A., El-Hajj Fuleihan G., Josse ... 80 (6): 1697S-1705S. CS1 maint: Multiple names: authors list (link) NHS Choice - Rickets Diagnoses Cheema, Jugesh I.; Grissom, ... 157 (3788): 501-6. doi:10.1126/science.157.3788.501. PMID 6028915. Barr, Sharma; Macdonald H.M., Sheehy T., Novotny R., ...
Phytase is an enzyme that is able to break down the otherwise indigestible phytic acid, making it available to the pig. The ... "Pigs expressing salivary phytase produce low-phosphorus manure". Nature Biotechnology. 19 (8): 741-5. doi:10.1038/90788. PMID ... The Enviropig is a genetically modified Yorkshire pig that expresses phytase in its saliva. Grains, such as corn and wheat, ... 6 (4): 245-52. doi:10.1007/s10123-003-0144-x. PMID 12955583. Mooney, H. A; Cleland, E. E (2001). "The evolutionary impact of ...
These pigs produced the enzyme phytase, which breaks down the indigestible phosphorus, in their saliva. The enzyme was ... On 6 February 2009, the U.S. Food and Drug Administration approved the first human biological drug produced from such an animal ... 96 (6): 1851-55. doi:10.1002/jsfa.7523. PMID 26536836. It is therefore not surprising that efforts to require labeling and to ... 30 (6): 1336-43. doi:10.1016/j.biotechadv.2012.02.006. PMID 22361646. "Aquabounty Cleared to Sell Salmon in USA for Commercial ...
Other GM crops are Phytaseed Canola varieties with phytase, sulfonylurea herbicide tolerant soybean and drought tolerant corn ( ... BASF offer polyamide 6 and polyamide 6,6 polymers as well as precursors. 6. Biodegradable plastics BASF was a pioneer in ... 6 March 2009. Retrieved 6 March 2009. "Integration of Ciba". BASF Report 2009. Retrieved 11 May 2015. [self-published source] " ... BASF (Badische Anilin- und Soda-Fabrik, or, in English, Baden Aniline and Soda Factory) was founded on 6 April 1865 in Mannheim ...
Aspergillus niger is used to produce recombinant phytase, an enzyme added to animal feeds to improve absorption of phosphorus. ... 42 (6): 574-81. doi:10.1080/10826068.2012.672943. PMID 23030468. Ina, K; Kataoka, T; Ando, T (2013). "The use of lentinan for ... 29 (6): 597-607. doi:10.1016/S0305-1978(00)00100-9. ISSN 0305-1978. PMID 11336809. Juárez-Montiel M, Ruiloba de León S, Chávez- ... 5-6): 318-24. PMID 9705612. "Maitake Mushroom". Complementary and Alternative Medicine : Diet and Nutrition. American Cancer ...
Enviropigs produce the enzyme phytase in the salivary glands that is secreted in the saliva. In 2006 the scientists from ... 7 (6): 525-539. doi:10.1016/S0963-6897(98)00043-8. PMID 9853581. Vollrath, F.; Knight, D. (2001). "Liquid crystalline spinning ...
"The Influence of Soaking and Germination on the Phytase Activity and Phytic Acid Content of Grains and Seeds Potentially Useful ... Retrieved 6 October 2016. Ramdath D, Renwick S, Duncan AM (2016). "The Role of Pulses in the Dietary Management of Diabetes". ... The hull of lentils usually accounts for 6 to 7% of the total seed weight, which is lower than most legumes. Lentil flour can ... doi:10.1016/s0308-8146(01)00332-6. US Department of Agriculture, National Nutrient Database for Standard Reference Release 28 ( ...
Transgenic maize plants expressing a fungal phytase gene. Transgenic Research *^ Achieving successful deployment of Bt rice. ... Majs genetisk modificeret med genet for enzymet phytase fra skimmelsvampen Aspergillus niger.[18] ... Genentech (6. september 1978). "The insulin synthesis is the first laboratory production DNA technology". Pressemeddelelse. ... En anden måde at udvikle fødemidler på, er at fjerne giftige stoffer fra planter.[6] ...
... on Replacement of Fish Meal with Plant Based Diet Supplemented with Dietary Cell Bound Phytase of Pichia anomala" (PDF). ... on Replacement of Fish Meal with Plant Based Diet Supplemented with Dietary Cell Bound Phytase of Pichia anomala" (PDF). ... It is 6 km from Baramulla. There are two bridges which connect the village to Kanispora via Ladoora, and Kawaja Bagh via ...
There are various enzymes such as phosphatases, nucleases and phytase involved for the degradation. Some of the abiotic ... doi:10.1007/s12665-012-1975-6. Ajmone-Marsan. F et al. (2005) Phosphorus transformations under reduction in long-term manured ...
Phytase *Beta-propeller phytase. *Inositol-phosphate phosphatase *IMPA1, IMPA2, IMPA3. *Protein phosphatase: Protein tyrosine ... 26 (1): 6-16. doi:10.1038/sj.ijo.0801858. PMID 11791141.. *. Smih F, Rouet P, Lucas S, et al. (2002). "Transcriptional ... 31 (Pt 6): 1120-4. doi:10.1042/BST0311120. PMID 14641008.. *. Holm C, Kirchgessner TG, Svenson KL, et al. (1988). "Hormone- ... 241 (4872): 1503-6. doi:10.1126/science.3420405. PMID 3420405.. *. Levitt RC, Liu Z, Nouri N, et al. (1995). "Mapping of the ...
"The Influence of Soaking and Germination on the Phytase Activity and Phytic Acid Content of Grains and Seeds Potentially Useful ... Retrieved 6 October 2016.. *^ Zhang, Bing; Deng, Zeyuan; Tang, Yao; Chen, Peter; Liu, Ronghua; Ramdath, D. Dan; Liu, Qiang; ... Source: FAOSTAT[6]. In 2016, global production of lentils was 6.3 million tonnes, led by Canada with 51% and India with 17% of ... doi:10.1016/s0308-8146(01)00332-6.. *^ US Department of Agriculture, National Nutrient Database for Standard Reference Release ...
Finase.ec is an E.coli-derived 6-phytase developed for swine and poultry feed. Learn how it can benefit the animal by releasing ... An E.coli-derived 6-phytase developed specifically for use in swine and poultry feeds.. *Degrades the phytate found in the ...
A full-length phytase cDNA was amplified from the germinating seedlings by splicing by overlap extension (SOE)-PCR and its ... Owing to a large number of E. coli low-usage codons in soybean phytase gene, the modified gene was cloned into a prokaryotic ... Phytic acid, the major storage form of phosphorus in plant seeds is degraded by the phytases to yield inositol and free ... Bioassay of the crude expression product in E. coli revealed a functional phytase gene, showing a great potential for ...
Furthermore, phytase at ${\geq}750FTU$ resulted in AID of total AA commensurate to that of PC fed birds and at ${\geq}1,000FTU ... Phytase response in NC birds was linear (p<0.05) with 2,000 FTU showing the greatest improvement on body weight gain (20%), ... phytase expressed in Trichoderma reesei. Diets were: positive control (PC) adequate in nutrients and negative control (NC) diet ... Phytase; A total of 420 day-old male Ross chicks were weighed at d 1 of life and assigned to test diets to assess the efficacy ...
This research was conducted to determine the effects of feeding different P levels with and without phytase supplementation on ... and these 2 diets supplemented with phytase at 600 phytase units/kg (nPP and Ca were reduced by 0.094% in diets with phytase). ... There was no main effect of phytase on BBS, but BBS was decreased in the broilers fed the LCaP diet with phytase addition (nPP ... Total P, SP, and RSP were decreased (P , 0.01) in the litter of broilers fed phytase. These data indicate that phytase ...
Safety and efficacy of Natuphos® E (6-phytase) as a feed additive for avian and porcine species. Number of Pages: 35 safety, ... 6-phytase, poultry and pigs First published in the EFSA Journal: 14 November 2017 Adopted: 28 September 2017 Last Updated: 15 ...
Other names in common use include 1-phytase, phytase, phytate 1-phosphatase, and phytate 6-phosphatase. This enzyme ... In enzymology, a 3-phytase (EC 3.1.3.8) is an enzyme that catalyzes the chemical reaction myo-inositol hexakisphosphate + H2O ... the inositol pentaphosphate products of Aspergillus ficuum phytases". J. Bacteriol. 112 (1): 434-8. PMC 251429 . PMID 4342816. ... 6-pentakisphosphate and phosphate. This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric ...
RE: The tariff classification of Phytase-based enzyme preparations from Mexico. Dear Mr. Correia:. In your letter dated May 8, ... 6-phytase is found in both plants and animals, and hydrolyses excess dietary phytic acid. One of the preparations is composed ... The subject products consist of two enzyme preparations, each containing a genetically modified form of the enzyme, 6-phytase, ...
China Phytases Market Research Report 2017 is a market research report available at US $3400 for a Single User PDF License from ... 1.2.2 China Phytases Sales (MT) Market Share by Type in 2016. 1.2.3 Granular Phytases. 1.2.4 Powder Phytases. 1.2.5 Liquid ... Table Phytases Sales (MT) by Application (2016-2022). Figure Product Picture of Phytases. Table China Phytases Sales (MT) and ... 1 Phytases Overview. 1.1 Product Overview and Scope of Phytases. 1.2 Classification of Phytases by Product Category. 1.2.1 ...
3-Phytase from Aspergillus niger expressed in Aspergillus niger; 1. EXPLANATION; 2. BIOLOGICAL DATA; 3. DIETARY EXPOSURE; 4. ... 3-Phytase from Aspergillus niger expressed in Aspergillus niger; 1. EXPLANATION; 2. BIOLOGICAL DATA; 3. DIETARY EXPOSURE; 4. ... EXPLANATION; 2. BIOLOGICAL DATA; 3. DIETARY EXPOSURE; 4. COMMENTS; 5. EVALUATION; 6. REFERENCES; Safety evaluations of groups ... 6. REFERENCES."@en ;. schema:description "This volume contains monographs prepared at the seventy-sixth meeting of the Joint ...
... phytase) as a feed additive for laying hens and other laying birds. Following a request from the European Commission, the EFSA ...
The 2 diets consisted of an industry diet (IND) and a low phosphorus + 600 U of phytase/kg (LP) diet. A subsample of birds was ... 6-Phytase / metabolism. Age Distribution. Animal Feed / analysis. Animal Nutritional Physiological Phenomena. Animals. Diet / ...
Kiarie, E.; Woyengo, T.; Nyachoti, C. M. (6 May 2015). "Efficacy of New 6-Phytase from Buttiauxella spp. on Growth Performance ... doi:10.1016/S0721-9571(81)80016-6. Kämpfer, Peter. "Buttiauxella". Wiley Online Library. doi:10.1002/9781118960608.gbm01139. ...
Learn more about the health benefits of Phytase. ... Phytase is an enzyme necessary for the digestive process, as ... Phytase activity is measured in FTU or phytase units; the higher the number, the more active the enzyme is. Phytase units are ... Where Can I Find the Best Source of Phytase?. VeganZyme® contains a 100% vegan form of phytase thats produced by the natural ... "Fabulous Phytase: phytase enzyme proving helpful to poultry producers and environment." Highlights of Agricultural Research. ...
The straw-derived fungal phytase obtained in the present study was higher than phytase produced from other crop residues such ... Solid-state fermentation (SSF) of wheat straw using Aspergillus ficuum was carried out to produce phytase. Phytase production ... Addition of glucose as an inducer and ammonium sulfate enhance phytase production considerably. Maximum phytase activity of ... Response surface approach for optimizing phytase production. Response surface method (RSM) was applied to optimize phytase ...
Phytase (myo-inositol hexaphosphate phosphohydrolase) belongs to phosphatases. It catalyzes the hydrolysis of phytate to less- ... 2003). Phytase activity in sourdough lactic acid bacteria: Purification and characterization of a phytase from Lactobacillus ... In the present study, phytase isolation from the Lactobacillus coryniformis strain, isolated from Lor cheese sources, phytase ... Phytase Enzyme purification Lactobacillus coryniformis Metal ions This is a preview of subscription content, log in to check ...
Here, we aim to distinguish the effects of mineral P, Ca and phytase on the composition of microbial communities present in the ... Here, we aim to distinguish the effects of mineral P, Ca and phytase on the composition of microbial communities present in the ... Phytase supplementation in broiler diets is a common practice to improve phosphorus (P) availability and to reduce P loss by ... Phytase supplementation in broiler diets is a common practice to improve phosphorus (P) availability and to reduce P loss by ...
Improve iron status of piglets with phytase. Author: Dr Peter Wilcock and Dr Jae Kim, AB Vista Dec 15. ... Improve iron status of piglets with phytase On Dec 15. In Piglets ...
Commission Regulation (EC) No 1142/2007 of 1 October 2007 concerning the authorisation of a new use of 3-phytase (Natuphos) as ... 1141/2007 der Kommission vom 1. Oktober 2007 zur Zulassung von 3-Phytase (ROVABIO PHY AP und ROVABIO PHY LC) als ... Commission Regulation (EC) No 1141/2007 of 1 October 2007 concerning the authorisation of 3-phytase (ROVABIO PHY AP and ROVABIO ... 1142/2007 der Kommission vom 1. Oktober 2007 zur Zulassung eines neuen Verwendungszwecks von 3-Phytase (Natuphos) als ...
Ákvörðun framkvæmdastjórnarinnar frá 6. desember 2007 um breytingu á ákvörðun 2002/627/EB um að koma á fót evrópskum hópi ... Kommisjonsvedtak av 6. desember 2007 om fastsetjing av ein modell for listene over einingar som er godkjende av medlemsstatane ... Commission Regulation (EC) No 1500/2007 of 18 December 2007 of concerning the authorisation of a new use of 6-phytase EC 3.1. ... Kommisjonsbeslutning av 6. desember 2007 om endring av kommisjonsbeslutning 2002/627/EF om opprettelse av Gruppen av europeiske ...
Bacteria in the intestinal tract may produce phytase enzymes (rumen bacteria produce a lot of phytase). Phytase enzyme may be ... phytases present in feed ingredients, (2) exogenous microbial phytases added to the feed, (3) phytases produced by endogenous ... Phytases. Phytases are enzymes that are able to catalyze the hydrolysis of the phosphate ester bonds of phytin. The action of ... Phytase enzymes may be added to the feed as exogenous sources. The phytase enzyme must come from someplace. ...
Four phytase producing bacterial isolates (phytase activity in the range of 0.076-0.174 U/mL), identified as Advenella species ... Evaluation of Phytase Producing Bacteria for Their Plant Growth Promoting Activities. Prashant Singh,1 Vinod Kumar,1,2 and ... Received 31 July 2013; Revised 5 November 2013; Accepted 6 November 2013; Published 23 January 2014 ...
229940085127 phytase Drugs 0 claims description 8 * 241000756502 Nocardiopsis alkaliphila YIM 80379 Species 0 abstract ... 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0 description 6 * 229930001578 L-Methionine Natural products 0 description 6 ... 241000756483 Nocardiopsis lucentensis DSM 44048 Species 0 abstract description 6 * 238000006460 hydrolysis Methods 0 claims ... 244000005700 microbiome Species 0 description 6 * 125000002924 primary amino group Chemical group data:image/svg+xml;base64, ...
In the present case, the sensitising phytase was an A. awamori 3-phytase expressed in T. reesei. This enzyme was detected in ... On the particular factory floor, aerosolised phytase (curtain of vaporised phytase) is added to the animal feed by a conveyor ... Blood examination showed high immunoglobulin G levels to phytase.. After treatment and cessation of phytase contact the patient ... 3 while the phytase concentration was 8.7-38.4 µg·m−3 (16.5-38.7 µg·mg−1, n = 8). On the other floors, phytase concentrations ...
... phytase activity has been increased in barely seeds by expression of phytase gene [HvPAPhy_a (101)]. Essential amino acid ... Austin-Phillips S, Koegel RG, Straub RJ, Cook M. Animal Feed Compositions Containing Phytase Derived from Transgenic Alfalfa ... Generation of transgenic wheat (Triticum aestivum L.) for constitutive accumulation of an Aspergillus phytase. Mol Breed (2000 ... Bioavailability of iron has been increased by expressing soybean ferritin and Aspergillus phytase (91), soybean ferritin (92), ...
Other fungal phytases such as those disclosed in JP 11000164. (Penicillium phytase), or WO98/13480. (Monascus anka phytase); ... an allelic variant of the phytase of (i)-(ix);. *xiii. a fragment of the phytase of (i) - (ix) that retains phytase activity; ... FTU is for determining phytase activity in feed and premix.. Preferred examples of phytases are microbial phytases, such as ... Three different types of phytases are known: A so-called 3-phytase (alternative name 1-phytase; a myo-inositol hexaphosphate 3- ...
  • The global Animal Feed Phytase market is valued at xx million US$ in 2017 and will reach xx million US$ by the end of 2025, growing at a CAGR of xx% during 2018-2025. (emailwire.com)
  • To study and analyze the global Animal Feed Phytase market size (value & volume) by company, key regions, products and end user, breakdown data from 2013 to 2017, and forecast to 2025. (emailwire.com)
  • Such research in this scientific literature over the period 2002 - 2017 of poultry, for instance, has revealed the most phytase suppliers, except OptiPhos, overestimated the matrix value by up to 25% and might thereby lead to under performance of the animals (Figure 2). (huvepharma.com)
  • Diets were: positive control (PC) adequate in nutrients and negative control (NC) diet (40% and 17% less available phosphorous (P) and calcium (Ca), respectively) supplemented with 6 levels of phytase 0, 250, 500, 750, 1,000, and 2,000 phytase units (FTU)/kg of diet. (koreascience.or.kr)
  • The levels of phytase in plants have shown to increase by several orders of magnitude during germination. (happywheelsonlinegame.net)
  • Recently there has been interest in "super-dosing" with higher levels of phytase (2500-5000 phytase units/kg feed) to more quickly and completely break down organic phosphates in p[lant feedstuffs and increase the availability of nutrients needed for growth and normal physiological functions. (usda.gov)
  • [ 1 ] Recent research shows that supplementing with phytase can significantly increase the body's ability to absorb and assimilate vital minerals such as calcium, magnesium, and iron. (globalhealingcenter.com)
  • The heterodimer is definitely identified by the E210 antibody [6 7 S100A8/A9 heterodimerization is not dependent on calcium but formation of heterotetramers is definitely calcium dependent. (exposed-skin-care.net)
  • Alkaline phytases (also called β-propeller phytases) are active at neutral or slightly alkaline conditions, calcium-dependent, and are quite thermostable so as to withstand the high temperatures during the pelleting of animal feeds. (avhandlingar.se)
  • To address the issue of environmental emissions, we established transgenic pigs harboring a single-copy quad-cistronic transgene and simultaneously expressing three microbial enzymes, β-glucanase, xylanase, and phytase in the salivary glands. (elifesciences.org)
  • Phytase is produced naturally by several strains of bacteria, yeast, and filamentous fungi. (springer.com)
  • 2003). Phytase activity in sourdough lactic acid bacteria: Purification and characterization of a phytase from Lactobacillus sanfranciscensis CB1. (springer.com)
  • For the production of phytases, variety of natural and recombinant expression systems employing bacteria, yeast and fungi are exploited to enhance the overall productivity. (mnpublishers.com)
  • 2001). The major phytase producers are fungi, bacteria and yeast (Pandey et al. (happywheelsonlinegame.net)
  • These "probiotics" (literally, "pro life") make great residents in dairy products because dairy products need to be refrigerated and have a short shelf life, in addition to being a commonly consumed source of nutrients, enabling consumers to reap the many healthy benefits these bacteria provide in improving gastrointestinal health (6,11). (kenyon.edu)
  • Characterization and expression of codon optimized soybean phytase gene in E. coli. (bvsalud.org)
  • Owing to a large number of E. coli low-usage codons in soybean phytase gene, the modified gene was cloned into a prokaryotic expression vector pET-28a (+) and its expression in E. coli was confirmed by SDS-PAGE and Western blot analysis. (bvsalud.org)
  • Bioassay of the crude expression product in E. coli revealed a functional phytase gene, showing a great potential for developing low phytate transgenic soybean through its seed-specific overexpression in the early stages of seed development. (bvsalud.org)
  • as-fed basis) in the presence or absence of Escherichia coli -derived phytase (500 FTU/kg), until birds were 21 days of age. (allaboutfeed.net)
  • The present invention relates to a phytase which has at least 70% identity to a phytase derived from E. coli and comprises at least one modification as compared to this phytase. (patentsencyclopedia.com)
  • To optimize P utilization in the animal, it is of great relevance to link the intermediate products of phytate degradation to phytases of different origins. (deepdyve.com)
  • Research has shown that not all phytases are equally resistant against this degradation by pepsin, which means that the full effectivity of these phytases in the gizzard / stomach cannot be achieved, even though they might have the right pH profile. (huvepharma.com)
  • Inclusion of phytase, at both 500 and 1,000 FTU/kg, resulted in lower IP6 and the accumulation of lower IP ester (IP3-5) concentrations in all GI segments (P (ovid.com)
  • 1) or 1,000 FTU phytase/kg (Exp. (vu-wien.ac.at)
  • A total of 350 Youxian Sheldrakes (7d old) were randomly divided into 5 treatment groups: positive control (PC) group has adequate available P diet (0.42% and 0.38%, starter and grower), negative control (NC) group were deficient in available P (0.32% and 0.28%, starter and grower) and NC diet was supplemented with 3 levels of microbial phytase (500, 750, and 1,000 U/kg). (ajas.info)
  • Recombinant fungal phytase, a glycoprotein designed for secretion, was delivered to the same compartment, with no trace of the molecule in the apoplast. (plantphysiol.org)
  • The localization of human serum albumin and phytase was compared to that of recombinant legumin, which contains structural targeting information directing it to the vacuole. (plantphysiol.org)
  • The focus has been directed to (1) developing a new kinetic method to determine the phytase activity, (2) cloning, expression and production of the recombinant phytase from Bacillus sp. (avhandlingar.se)
  • A close investigation of the effect of divalent metal ions on the activity and stability of the recombinant phytase was performed. (avhandlingar.se)
  • The speed of hydrolysis of phytate by a phytase (the V max ) is largely dependent on its pH profile and pepsin resistance and can be determined during in vitro enzymatic studies (the so-called Michaelis Menten kinetic studies). (huvepharma.com)
  • The global Phytases market is valued at 421.8 million US$ in 2020 is expected to reach 624.5 million US$ by the end of 2026, growing at a CAGR of 5.7% during 2021-2026. (marketresearchglobe.com)
  • Various physical and chemical factors have been known to affect the growth and the production of phytase. (scirp.org)
  • Physiologically, the reason for this unavailability is due to the very low phytase activity found in the digestive tract of monogastric animals. (ajas.info)
  • In the present study, phytase isolation from the Lactobacillus coryniformis strain, isolated from Lor cheese sources, phytase purification and characterization were studied. (springer.com)
  • S.C. Johnson, M. Yang, P.P.N. Murthy (2010) Heterologous expression and functional characterization of a plant alkaline phytase in Pichia pastoris. (mtu.edu)
  • B.G. Garchow, S.P. Jog, B. D. Mehta, J.M. Monosso (posthumous), P.P.N. Murthy (2006) Alkaline phytase from Lilium longiflorum: purification and structural characterization. (mtu.edu)
  • Mimi Yang, Sasha Teymorian, Philip Olivares, Pushpalatha P.N. Murthy (2015) Extracellular expression of alkaline phytase in Pichia pastoris: the GAP promoter is more efficient than the AOX1 promoter. (mtu.edu)
  • M. Yang, S. Johnson, P.P.N. Murthy (2012) Enhancement of alkaline phytase production in Pichia pastoris: influence of gene dosage, sequence optimization and expression temperature. (mtu.edu)
  • Bakul Dhagat Mehta, Sonali P. Jog, Steven C. Johnson and Pushpalatha P.N. Murthy (2006) Lily pollen alkaline phytase is a histidine phosphatase similar to mammalian multiple inositol polyphosphate phosphatase (MINPP), Phytochemistry, 67, 1874-1886. (mtu.edu)
  • Most plant phytases belong to either an acidic group with a pH optimum around 5.5 or to an alkaline group with a pH optimum around 8.0. (happywheelsonlinegame.net)
  • Alkaline phytases have several other potential applications. (avhandlingar.se)
  • Alkaline phytases have a lower specific activity than their acid counterparts. (avhandlingar.se)