6-Phytase: An enzyme that catalyzes the conversion of myo-inositol hexakisphosphate and water to 1L-myo-inositol 1,2,3,4,5-pentakisphosphate and orthophosphate. EC 3.1.3.26.Phytic Acid: Complexing agent for removal of traces of heavy metal ions. It acts also as a hypocalcemic agent.Phosphorus, Dietary: Phosphorus used in foods or obtained from food. This element is a major intracellular component which plays an important role in many biochemical pathways relating to normal physiological functions. High concentrations of dietary phosphorus can cause nephrocalcinosis which is associated with impaired kidney function. Low concentrations of dietary phosphorus cause an increase in calcitriol in the blood and osteoporosis.Aspergillus niger: An imperfect fungus causing smut or black mold of several fruits, vegetables, etc.Phosphorus: A non-metal element that has the atomic symbol P, atomic number 15, and atomic weight 31. It is an essential element that takes part in a broad variety of biochemical reactions.Animal Feed: Foodstuff used especially for domestic and laboratory animals, or livestock.Soybeans: An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS.Digestion: The process of breakdown of food for metabolism and use by the body.Coccyx: The last bone in the VERTEBRAL COLUMN in tailless primates considered to be a vestigial tail-bone consisting of three to five fused VERTEBRAE.Animal Nutritional Physiological Phenomena: Nutritional physiology of animals.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Zea mays: A plant species of the family POACEAE. It is a tall grass grown for its EDIBLE GRAIN, corn, used as food and animal FODDER.Biological Availability: The extent to which the active ingredient of a drug dosage form becomes available at the site of drug action or in a biological medium believed to reflect accessibility to a site of action.Dietary Supplements: Products in capsule, tablet or liquid form that provide dietary ingredients, and that are intended to be taken by mouth to increase the intake of nutrients. Dietary supplements can include macronutrients, such as proteins, carbohydrates, and fats; and/or MICRONUTRIENTS, such as VITAMINS; MINERALS; and PHYTOCHEMICALS.Weaning: Permanent deprivation of breast milk and commencement of nourishment with other food. (From Stedman, 25th ed)Diet: Regular course of eating and drinking adopted by a person or animal.Minerals: Native, inorganic or fossilized organic substances having a definite chemical composition and formed by inorganic reactions. They may occur as individual crystals or may be disseminated in some other mineral or rock. (Grant & Hackh's Chemical Dictionary, 5th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Random Allocation: A process involving chance used in therapeutic trials or other research endeavor for allocating experimental subjects, human or animal, between treatment and control groups, or among treatment groups. It may also apply to experiments on inanimate objects.Weight Gain: Increase in BODY WEIGHT over existing weight.
(1/247) Expression of an Aspergillus niger phytase gene (phyA) in Saccharomyces cerevisiae.

Phytase improves the bioavailability of phytate phosphorus in plant foods to humans and animals and reduces phosphorus pollution of animal waste. Our objectives were to express an Aspergillus niger phytase gene (phyA) in Saccharomyces cerevisiae and to determine the effects of glycosylation on the phytase's activity and thermostability. A 1.4-kb DNA fragment containing the coding region of the phyA gene was inserted into the expression vector pYES2 and was expressed in S. cerevisiae as an active, extracellular phytase. The yield of total extracellular phytase activity was affected by the signal peptide and the medium composition. The expressed phytase had two pH optima (2 to 2.5 and 5 to 5.5) and a temperature optimum between 55 and 60 degrees C, and it cross-reacted with a rabbit polyclonal antibody against the wild-type enzyme. Due to the heavy glycosylation, the expressed phytase had a molecular size of approximately 120 kDa and appeared to be more thermostable than the commercial enzyme. Deglycosylation of the phytase resulted in losses of 9% of its activity and 40% of its thermostability. The recombinant phytase was effective in hydrolyzing phytate phosphorus from corn or soybean meal in vitro. In conclusion, the phyA gene was expressed as an active, extracellular phytase in S. cerevisiae, and its thermostability was affected by glycosylation.  (+info)

(2/247) Phytase improves iron bioavailability for hemoglobin synthesis in young pigs.

Dietary phytase supplementation improves bioavailabilities of phytate-bound minerals such as P, Ca, and Zn to pigs, but its effect on Fe utilization is not clear. The efficacy of phytase in releasing phytate-bound Fe and P from soybean meal in vitro and in improving dietary Fe bioavailability for hemoglobin repletion in young, anemic pigs was examined. In Exp. 1, soybean meal was incubated at 37 degrees C for 4 h with either 0, 400, 800, or 1,200 units (U) of phytase/kg, and the released Fe and P concentrations were determined. In Exp. 2, 12 anemic, 21-d-old pigs were fed either a strict vegetarian, high-phytate (1.34%) basal diet alone, or the diet supplemented with 50 mg Fe/kg diet (ferrous sulfate) or phytase at 1,200 U/kg diet (Natuphos, BASF, Mt. Olive, NJ) for 4 wk. In Exp. 3, 20 anemic, 28-d-old pigs were fed either a basal diet with a moderately high phytate concentration (1.18%) and some animal protein or the diet supplemented with 70 mg Fe/kg diet, or with one of two types of phytase (Natuphos or a new phytase developed in our laboratory, 1,200 U/kg diet) for 5 wk. In Exp. 2 and 3, diets supplemented with phytase contained no inorganic P. In Exp. 1, free P concentrations in the supernatant increased in a phytase dose-dependent fashion (P<.05), whereas free Fe concentrations only increased at the dose of 1,200 U/kg (P<.10). In Exp. 2 and 3, dietary phytase increased hemoglobin concentrations and packed cell volumes over the unsupplemented group; these two measures, including growth performance, were not significantly different than those obtained with dietary supplemental Fe. In conclusion, both sources of phytase effectively degraded phytate in corn-soy diets and subsequently released phytate-bound Fe from the diets for hemoglobin repletion in young, anemic pigs.  (+info)

(3/247) Occupational IgE sensitisation to phytase, a phosphatase derived from Aspergillus niger.

OBJECTIVE: Phytase is a phosphatase derived from Aspergillus niger that enhances phosphate bioavailability in the gut, and therefore has been increasingly used as an animal feed additive since the early 1990s. The aim of this study was to assess whether work related respiratory symptoms among workers in a so called premix factory producing animal feed additives, could be due to type I (mediated by immunoglobulin E (IgE) allergic sensitisation to phytase. METHODS: Preparations of specific IgE against phytase as used in the factory were assessed by enzyme immunoassay (EIA) in serum samples of 11 exposed workers who regularly handled the enzyme, in 11 office and laboratory workers of the same plant (non-exposed internal controls), and in 19 laboratory animal workers as external controls. The factory workers also completed a questionnaire on common and work related respiratory symptoms. RESULTS: Depending on the cut off level in the EIA for IgE, and the preparation used as coated allergen, antiphytase sensitisation was found in one to four of the 19 external controls, in one to five of the 11 internal controls, and in four to 10 of the 11 exposed workers. Strongest IgE reactions were found in four exposed workers who reported work related respiratory symptoms, particularly wheezing, and in one internal control who possibly had become sensitised because the structure of the factory building did not preclude airborne exposure in the offices and corridors of the plant. Experiments with inhibition EIA for IgE showed that (a) phytase of another commercial source was only partially cross reactive with phytase as used in the premix factory, and (b) phytase used as an animal feed additive did not cross react with common mould extracts, except for extracts from the species of origin, Aspergillus niger. The amount of IgE binding phytase in Aspergillus niger was estimated to be between 0.1% and 1% of the extractable mould proteins. CONCLUSIONS: Phytase is a potentially important new occupational allergen causing specific IgE immune responses among exposed workers. Such IgE sensitisation could probably be the cause of work related asthmatic and other respiratory symptoms if no effective measures are taken to prevent airborne occupational exposure at sites where phytase is handled, particularly during addition of enzyme preparations to animal feed.  (+info)

(4/247) Dephosphorylation of phytate by using the Aspergillus niger phytase with a high affinity for phytate.

A phytase (EC 3.1.3.8) with a high affinity for phytic acid was found in Aspergillus niger SK-57 and purified to homogeneity in four steps by using ion-exchange chromatography (two types), gel filtration, and chromatofocusing. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified enzyme gave a single stained band at a molecular mass of approximately 60 kDa. The Michaelis constant of the enzyme for phytic acid (18.7 +/- 4.6 microM) was statistically analyzed. In regard to the orthophosphate released from phytic acid, a significant difference between a low K(m) phytase from A. niger SK-57 and a high K(m) phytase from Aspergillus ficuum was recognized.  (+info)

(5/247) Comparative enzymatic hydrolysis of phytate in various animal feedstuff with two different phytases.

Bacillus amyloliquefaciens DS11 phytase (DS11 phytase) and Aspergillus ficuum phytase (AF phytase) activities were investigated by measuring the release of phosphate from phytate in animal feedstuff such as wheat bran, corn meal, soybean meal and rice flour at pH 5 and 7. In all the tested feedstuff, the enzymatic activity of DS11 phytase was more active at pH 7, but that of AF phytase was more active at pH 5. From these results, the phytate in the gastrointestinal tract could be degraded in the small intestine or stomach by DS11 or AF phytase, respectively. In conclusion, the results presented in this paper indicated that different combination ratios of DS11 and AF phytase, depending on the kind of feedstuff, might effectively induce more enzymatic activity both in the stomach and small intestine in terms of the pH of the gastrointestinal tract.  (+info)

(6/247) From DNA sequence to improved functionality: using protein sequence comparisons to rapidly design a thermostable consensus phytase.

Naturally-occurring phytases having the required level of thermostability for application in animal feeding have not been found in nature thus far. We decided to de novo construct consensus phytases using primary protein sequence comparisons. A consensus enzyme based on 13 fungal phytase sequences had normal catalytic properties, but showed an unexpected 15-22 degrees C increase in unfolding temperature compared with each of its parents. As a first step towards understanding the molecular basis of increased heat resistance, the crystal structure of consensus phytase was determined and compared with that of Aspergillus niger phytase. Aspergillus niger phytase unfolds at much lower temperatures. In most cases, consensus residues were indeed expected, based on comparisons of both three-dimensional structures, to contribute more to phytase stabilization than non-consensus amino acids. For some consensus amino acids, predicted by structural comparisons to destabilize the protein, mutational analysis was performed. Interestingly, these consensus residues in fact increased the unfolding temperature of the consensus phytase. In summary, for fungal phytases apparently an unexpected direct link between protein sequence conservation and protein stability exists.  (+info)

(7/247) Efficacy of yeast phytase in improving phosphorus bioavailability in a corn-soybean meal-based diet for growing pigs.

Crossbred barrows (n = 66; 6 wk old) were used in a 6-wk experiment to evaluate the efficacy of phytase from yeast or Aspergillus niger on performance, tibial characteristics, and serum inorganic P concentration. We also investigated the stability of these phytases in acidic solutions with pepsin, which simulated gastric conditions. Pigs were fed a P-adequate diet containing .34% nonphytate-P or a low-P diet containing .20% nonphytate-P. The low-P diet was supplemented with 0, 1,000, 2,000, or 4,000 phytase units (PU; the activity at optimal pH, i.e., pH 4.2 for yeast phytase and pH 5.5 for phytase from Aspergillus niger)/kg of yeast phytase, or 1,000 PU/kg phytase from Aspergillus niger. The graded level of yeast phytase linearly increased ADG (P = .047), tibial weight (P = .091), tibial density (P < .001), and P concentration in tibial cortex (P = .018). Aspergillus niger phytase also increased ADG (P = .022), serum inorganic P concentration (P < .001), tibial density (P = .007), and tibial P concentration (P = .025). The pigs given 1,000 PU/kg Aspergillus niger phytase showed greater ADG (P = .091), tibial density (P= .001), and tibial P concentration (P = .062) than those given 1,000 PU/kg yeast phytase. No measurements differed (P > .31) between the pigs given 1,000 PU/kg Aspergillus niger phytase and those given 4,000 PU/kg yeast phytase. These results suggested that yeast phytase improves bioavailability of P in the diet for growing pigs but the efficacy of yeast phytase is less than that of Aspergillus niger phytase. During incubation in acidic solutions with pepsin, yeast phytase (P < .001) lost more of its activity than Aspergillus niger phytase. This lesser stability of yeast phytase may be responsible for the poorer efficacy of yeast phytase than that of Aspergillus niger. In summary, supplementation of swine diets with yeast phytase is beneficial, but its efficacy is less than that of Aspergillus niger phytase.  (+info)

(8/247) Effects of dietary calcium:phosphorus ratios on apparent absorption of calcium and phosphorus in the small intestine, cecum, and colon of pigs.

Thirty-two crossbred barrows were used to investigate the effects of dietary Ca:total P (tP) ratios in phytase-supplemented diets on the apparent absorption of P and Ca in the small intestine, cecum, and colon. Three Ca:tP ratio treatments (1.5:1, 1.3:1, or 1.0:1) were created by adjusting the amount of ground limestone added to the basal low-P grower (.39% tP including .07% added inorganic P) and finisher (.32% tP without added inorganic P) diets. All low-P ratio diets were supplemented with Natuphos phytase at 500 units/kg. A positive control diet without phytase supplementation contained adequate P and Ca to meet dietary requirements. At 123 kg, the pigs were slaughtered and the contents of ileum, cecum, and colon were collected. Lowering the dietary Ca:tP ratio in the diets containing phytase linearly increased (P < .01) the apparent absorption (% and g/d) of P in the small intestine, but Ca absorption was not affected. Pigs fed the low-P diet with a Ca:tP ratio of 1.0:1 had an apparent absorption (g/d) of P or Ca similar to that of pigs fed the control diet, which was adequate in Ca and P. Averaged across all diets, the apparent absorption of P was highest when measured at the cecum, and the apparent absorption of Ca was highest when measured at the colon. In conclusion, lowering the dietary Ca:tP ratio to 1.0:1 in a low-P diet containing phytase increased the apparent absorption of P in the small intestine. Furthermore, a significant amount of P was absorbed in the cecum.  (+info)

*  Buttiauxella
Kiarie, E.; Woyengo, T.; Nyachoti, C. M. (6 May 2015). "Efficacy of New 6-Phytase from Buttiauxella spp. on Growth Performance ... doi:10.1016/S0721-9571(81)80016-6. Kämpfer, Peter. "Buttiauxella". Wiley Online Library. doi:10.1002/9781118960608.gbm01139. ...
*  4-phytase
Lim PE; Tate ME (1973). "The phytases. II. Properties of phytase fractions F 1 F 2 from wheat bran and the myoinositol ... In enzymology, a 4-phytase (EC 3.1.3.26) is an enzyme that catalyzes the chemical reaction myo-inositol hexakisphosphate + H2O ... Tomlinson RV; Ballou CE (1962). "Myoinositol polyphosphate intermediates in the dephosphorylation of phytic acid by phytase". ... phytase, and phytate 6-phosphatase. This enzyme participates in inositol phosphate metabolism. Johnson LF; Tate ME (1969). "The ...
*  List of MeSH codes (D08)
... map kinase kinase 6 MeSH D08.811.913.696.620.682.700.565.700 --- map kinase kinase 7 MeSH D08.811.913.696.620.682.700.567 --- ... map kinase kinase 6 MeSH D08.811.913.696.620.682.725.200.700 --- map kinase kinase 7 MeSH D08.811.913.696.620.682.725.300 --- ... cyclin-dependent kinase 6 MeSH D08.811.913.696.620.682.700.646.625 --- glycogen synthase kinase 3 MeSH D08.811.913.696.620.682. ... l-lysine 6-transaminase MeSH D08.811.913.477.700.700 --- ornithine-oxo-acid transaminase MeSH D08.811.913.477.700.800 --- ...
*  Phosphoric monoester hydrolases
... acid phosphatase alkaline phosphatase fructose-bisphosphatase glucose-6-phosphatase phosphofructokinase-2 phosphoprotein ... phosphatase calcineurin 6-phytase phosphodiesterase phosphatase Phosphoric Monoester Hydrolases at the US National Library of ...
*  3-phytase
Other names in common use include 1-phytase, phytase, phytate 1-phosphatase, and phytate 6-phosphatase. This enzyme ... In enzymology, a 3-phytase (EC 3.1.3.8) is an enzyme that catalyzes the chemical reaction myo-inositol hexakisphosphate + H2O ... the inositol pentaphosphate products of Aspergillus ficuum phytases". J. Bacteriol. 112 (1): 434-8. PMC 251429 . PMID 4342816. ... 6-pentakisphosphate and phosphate. This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric ...
*  Phytase
4-phytase (EC 3.1.3.26), and 5-phytase (EC 3.1.3.72). To date, most of the known phytases are 3-phytases or 4-phytases, only a ... such as phytases from Bacillus sp., Aspergillus sp., E. coli and those phytases belonging to the class of PTP-like phytases ... protein tyrosine phosphatase-like phytases (PTP-like phytases). Most of the known phytases belong to a class of enzyme called ... Most phytases are able to cleave five of the six phosphate groups from phytic acid. Phytases have been grouped based on the ...
*  List of EC numbers (EC 3)
3-phytase EC 3.1.3.9: glucose-6-phosphatase EC 3.1.3.10: glucose-1-phosphatase EC 3.1.3.11: fructose-bisphosphatase EC 3.1.3.12 ... 5-phytase EC 3.1.3.73: a-ribazole phosphatase EC 3.1.3.74: pyridoxal phosphatase EC 3.1.3.75: phosphoethanolamine/ ... 4-phytase EC 3.1.3.27: phosphatidylglycerophosphatase EC 3.1.3.28: ADP-phosphoglycerate phosphatase EC 3.1.3.29: N- ... Identical to EC 3.5.1.25, N-acetylglucosamine-6-phosphate deacetylase EC 3.5.1.81: N-acyl-D-amino-acid deacylase EC 3.5.1.82: N ...
*  5-phytase
As of late 2007, only the phytase purified from lily pollen had its structure solved, with PDB accession codes 1U24, 1U25, 1U26 ... Of the hundreds of phytase enzymes that have been characterized in the literature, only two have been characterized as 5- ... phytases. A histidine acid phosphatases purified from lily pollen and a protein tyrosine phosphatase-like phytase from ... In enzymology, a 5-phytase (EC 3.1.3.72) is an enzyme that catalyzes the chemical reaction myo-inositol hexakisphosphate + H2O ...
*  Phytic acid
Degradation of Phytate in Foods by Phytases in Fruit and Vegetable Extracts B.Q. PHILLIPPY AND C.J. WYATT Dendougui, Ferial; ... Ruminants are readily able to digest phytate because of the phytase produced by rumen microorganisms. In most commercial ... Dephytinisation with Intrinsic Wheat Phytase and Iron Fortification Significantly Increase Iron Absorption from Fonio ( ... "Phytases: attributes, catalytic mechanisms, and applications" (PDF). United States Department of Agriculture-Agricultural ...
*  Myceliophthora thermophila
Also expressed by this species are broad-specificity phytases that are efficient in breaking down phytic acid to be used for ... "Biochemical Characterization of Fungal Phytases (myo-Inositol Hexakisphosphate Phosphohydrolases) Catalytic Properties". Appl. ... 31 (6): 819-826. Wyss, M.; Brugger, R.; Kronenberger, A.; Remy, R.; Fimbel, R.; Oesterhelt, G.; Lehmann, M.; van Loon, A. (1999 ... 49 (8): 883-6. doi:10.3109/13693786.2011.584218. PMID 21619496. Destino, Lauren; Sutton, Deanna A; Helon, Anna L; Havens, Peter ...
*  Mucor racemosus
For example, it can produce a high yield of phytase, an important industrial enzyme. It also has an increased extracellular ... Bogar, B.; Szakacs, G.; Pandey, A.; Abdulhameed, S.; Linden, J.C.; Tengerdy, R.P. (4 April 2003). "Production of Phytase by ... 16 (6): 397-402. PMID 3242377. Zhao, Z; Li, L; Wan, Z; Chen, W; Liu, H; Li, R (April 2011). "Simultaneous detection and ... 40 (3): 181-6. doi:10.1111/j.1398-9995.1985.tb00214.x. PMID 4039540. Güneser, S; Atici, A; Köksal, F; Yaman, A (1994). "Mold ...
*  Pedobacter nyackensis
"A novel beta-propeller phytase from Pedobacter nyackensis MJ11 CGMCC 2503 with potential as an aquatic feed additive". Applied ... 59 (Pt 7): 1720-6. doi:10.1099/ijs.0.000158-0. PMID 19542109. Huang, Huoqing; Shao, Na; Wang, Yaru; Luo, Huiying; Yang, Peilong ...
*  Rickets
Pileggi VJ, De Luca HF, Steenbock H (September 1955). "The role of vitamin D and intestinal phytase in the prevention of ... 64 (6): 871-877. Mithal, A.; Wahl D.A., Bonjour J.P., Burckhardt T., Dawson-Hughes B., Eisman J.A., El-Hajj Fuleihan G., Josse ... 80 (6): 1697S-1705S. CS1 maint: Multiple names: authors list (link) NHS Choice - Rickets Diagnoses Cheema, Jugesh I.; Grissom, ... 157 (3788): 501-6. doi:10.1126/science.157.3788.501. PMID 6028915. Barr, Sharma; Macdonald H.M., Sheehy T., Novotny R., ...
*  Agricultural pollution
Phytase is an enzyme that is able to break down the otherwise indigestible phytic acid, making it available to the pig. The ... "Pigs expressing salivary phytase produce low-phosphorus manure". Nature Biotechnology. 19 (8): 741-5. doi:10.1038/90788. PMID ... The Enviropig is a genetically modified Yorkshire pig that expresses phytase in its saliva. Grains, such as corn and wheat, ... 6 (4): 245-52. doi:10.1007/s10123-003-0144-x. PMID 12955583. Mooney, H. A; Cleland, E. E (2001). "The evolutionary impact of ...
*  Genetically modified organism
These pigs produced the enzyme phytase, which breaks down the indigestible phosphorus, in their saliva. The enzyme was ... On 6 February 2009, the U.S. Food and Drug Administration approved the first human biological drug produced from such an animal ... 96 (6): 1851-55. doi:10.1002/jsfa.7523. PMID 26536836. It is therefore not surprising that efforts to require labeling and to ... 30 (6): 1336-43. doi:10.1016/j.biotechadv.2012.02.006. PMID 22361646. "Aquabounty Cleared to Sell Salmon in USA for Commercial ...
*  BASF
Other GM crops are Phytaseed Canola varieties with phytase, sulfonylurea herbicide tolerant soybean and drought tolerant corn ( ... BASF offer polyamide 6 and polyamide 6,6 polymers as well as precursors. 6. Biodegradable plastics BASF was a pioneer in ... 6 March 2009. Retrieved 6 March 2009. "Integration of Ciba". BASF Report 2009. Retrieved 11 May 2015. [self-published source] " ... BASF (Badische Anilin- und Soda-Fabrik, or, in English, Baden Aniline and Soda Factory) was founded on 6 April 1865 in Mannheim ...
*  Medicinal fungi
Aspergillus niger is used to produce recombinant phytase, an enzyme added to animal feeds to improve absorption of phosphorus. ... 42 (6): 574-81. doi:10.1080/10826068.2012.672943. PMID 23030468. Ina, K; Kataoka, T; Ando, T (2013). "The use of lentinan for ... 29 (6): 597-607. doi:10.1016/S0305-1978(00)00100-9. ISSN 0305-1978. PMID 11336809. Juárez-Montiel M, Ruiloba de León S, Chávez- ... 5-6): 318-24. PMID 9705612. "Maitake Mushroom". Complementary and Alternative Medicine : Diet and Nutrition. American Cancer ...
*  Genetically modified mammal
Enviropigs produce the enzyme phytase in the salivary glands that is secreted in the saliva. In 2006 the scientists from ... 7 (6): 525-539. doi:10.1016/S0963-6897(98)00043-8. PMID 9853581. Vollrath, F.; Knight, D. (2001). "Liquid crystalline spinning ...
*  Lentil
"The Influence of Soaking and Germination on the Phytase Activity and Phytic Acid Content of Grains and Seeds Potentially Useful ... Retrieved 6 October 2016. Ramdath D, Renwick S, Duncan AM (2016). "The Role of Pulses in the Dietary Management of Diabetes". ... The hull of lentils usually accounts for 6 to 7% of the total seed weight, which is lower than most legumes. Lentil flour can ... doi:10.1016/s0308-8146(01)00332-6. US Department of Agriculture, National Nutrient Database for Standard Reference Release 28 ( ...
*  Chakloo
... on Replacement of Fish Meal with Plant Based Diet Supplemented with Dietary Cell Bound Phytase of Pichia anomala" (PDF). ... on Replacement of Fish Meal with Plant Based Diet Supplemented with Dietary Cell Bound Phytase of Pichia anomala" (PDF). ... It is 6 km from Baramulla. There are two bridges which connect the village to Kanispora via Ladoora, and Kawaja Bagh via ...
*  Phosphorus cycle
There are various enzymes such as phosphatases, nucleases and phytase involved for the degradation. Some of the abiotic ... doi:10.1007/s12665-012-1975-6. Ajmone-Marsan. F et al. (2005) Phosphorus transformations under reduction in long-term manured ...
*  Thermomyces lanuginosus
The phytase of T. lanuginosus has optimum activity at 65 °C and a pH of 6.0. Differential scanning calorimetry has shown that ... There is therefore an interest in the use of phytases to break down the phytic acid and avoid this extra step. Four putative ... Berikten, Derya; Kivanc, Merih (2014-11-17). "OPTIMIZATION OF SOLID-STATE FERMENTATION FOR PHYTASE PRODUCTION BY Thermomyces ... phytase, and chitinase. These enzymes have chemical, environmental, and industrial applications due to their hydrolytic ...
*  Genetically modified livestock
These pigs produced the enzyme phytase, which breaks down the indigestible phosphorus, in their saliva. The enzyme was ... The wild-type salmon takes 24 to 30 months to reach market size (4-6 kg) whereas the producers of the GM salmon claim it ...
*  Sprouting
"The Influence of Soaking and Germination on the Phytase Activity and Phytic Acid Content of Grains and Seeds Potentially Useful ... 68 (6): 487-495. doi:10.1002/j.2050-0416.1962.tb01894.x. Shipard, Isabell (2005). How can I grow and use sprouts as living food ... 1989) found that the fibre content increased from 3.75% in unsprouted barley seed to 6% in 5-day sprouts." Crude Protein and ... doi:10.1007/s00217-006-0300-6. Diana, Marina; Quílez, Joan; Rafecas, Magdalena (September 2014). "Gamma-aminobutyric acid as a ...
*  Fermentation
Wang, H. L.; Swain, E. W.; Hesseltine, C. W. (1980). "Phytase of molds used in oriental food fermentation". Journal of Food ... ISBN 978-0-7484-0731-6. Voet, Donald; Voet, Judith G. (1995). Biochemistry (2nd ed.). New York, NY: John Wiley & Sons. ISBN 978 ...
*  Fungi in human culture
... phytases, alpha-glucuronidases, mannanases, and lipases. The fruiting bodies of many larger fungi such as the chanterelle and ... 10 (6): 717-735. doi:10.1080/09583150020011690. CS1 maint: Uses authors parameter (link) Lomer, C.J.; Bateman, R.P.; Johnson, D ... 9 (6): 729. doi:10.1111/J.1364-3703.2008.00500.X. PMID 19019002. Muir, Patricia. "Why does genetic diversity within and among ... doi:10.1016/S0269-915X(09)80073-6. Ostergaard S, Olsson L, Nielsen J. (2000). "Metabolic Engineering of Saccharomyces ...
The Effect of Calcium Level on Microbial Phytase Activity and Nutrient Balance in Swine  The Effect of Calcium Level on Microbial Phytase Activity and Nutrient Balance in Swine
The two additional experimental diets contained microbial phytase (750 phytase units/kg) and supplied either 0.8% or 0.4% ... Effects of Dietary Lysine and Microbial Phytase on Growth Performance and Nutrient Utilisation of Broiler Chickens2007 July;20( ... With added microbial phytase, ileal and total tract digestibility of total phosphorus were improved by 20.9 and 13.8 percentage ... Supplementation with microbial phytase significantly reduced fecal excretion of nitrogen and phosphorus and increased the ...
more infohttps://www.ajas.info/journal/view.php?number=19382
Construction of a Probiotic Lactic Acid Bacterium that Expresses Acid-Resistant Phytase Enzyme  Construction of a Probiotic Lactic Acid Bacterium that Expresses Acid-Resistant Phytase Enzyme
Phytase enzyme was isolated from cellular extracts of recombinant L. lactis, showing a 46 kDa band upon the SDS-PAGE analysis. ... Phytase enzyme is commonly added to poultry feed to improve digestibility and availability of phosphorus from plant sources. To ... Zymogram also confirmed the phytase activity of the 46 kDa band corresponding to the enzyme. An enzyme activity of 4.9 U mL-1 ... construct a probiotic with potential of phytate degradation, phytase gene (appA) from E. coli was cloned and transformed into ...
more infohttp://jast.modares.ac.ir/article_14809.html
Soybean cultivar S090130 - Patent # 8546653 - PatentGenius  Soybean cultivar S090130 - Patent # 8546653 - PatentGenius
... wherein the method comprises introducing a gene encoding a protein selected from the group consisting of phytase, ... 6. A soybean seed produced by the method of claim 5.. 7. A soybean plant, or a part thereof, produced by growing said seed of ... claim 6.. 8. The method of claim 5, wherein at least one of said soybean plants is transgenic.. 9. A method of producing an ...
more infohttp://www.patentgenius.com/patent/8546653.html
Finase EC: 6-phytase for Swine & Poultry Feed | AB Vista  Finase EC: 6-phytase for Swine & Poultry Feed | AB Vista
Finase.ec is an E.coli-derived 6-phytase developed for swine and poultry feed. Learn how it can benefit the animal by releasing ... An E.coli-derived 6-phytase developed specifically for use in swine and poultry feeds.. *Degrades the phytate found in the ...
more infohttps://www.abvista.com/Products/finaseec.aspx
Scientific Outputs | European Food Safety Authority  Scientific Outputs | European Food Safety Authority
Safety and efficacy of Natuphos® E (6-phytase) as a feed additive for avian and porcine species. Number of Pages: 35 safety, ... 6-phytase, poultry and pigs First published in the EFSA Journal: 14 November 2017 Adopted: 28 September 2017 Last Updated: 15 ...
more infohttp://www.efsa.europa.eu/en/publications/?subject=61826&f%5B0%5D=im_field_subject%3A62281
The effect of dietary phosphorus level and phytase supplementation on growth performance, bone-breaking strength, and litter...  The effect of dietary phosphorus level and phytase supplementation on growth performance, bone-breaking strength, and litter...
This research was conducted to determine the effects of feeding different P levels with and without phytase supplementation on ... and these 2 diets supplemented with phytase at 600 phytase units/kg (nPP and Ca were reduced by 0.094% in diets with phytase). ... There was no main effect of phytase on BBS, but BBS was decreased in the broilers fed the LCaP diet with phytase addition (nPP ... Total P, SP, and RSP were decreased (P , 0.01) in the litter of broilers fed phytase. These data indicate that phytase ...
more infohttp://www.biomedsearch.com/nih/effect-dietary-phosphorus-level-phytase/18420986.html
Buttiauxella - Wikipedia  Buttiauxella - Wikipedia
Kiarie, E.; Woyengo, T.; Nyachoti, C. M. (6 May 2015). "Efficacy of New 6-Phytase from Buttiauxella spp. on Growth Performance ... doi:10.1016/S0721-9571(81)80016-6. Kämpfer, Peter. "Buttiauxella". Wiley Online Library. doi:10.1002/9781118960608.gbm01139. ...
more infohttps://en.wikipedia.org/wiki/Buttiauxella
Diet and Cholesterol sub-cluster 32  Diet and Cholesterol sub-cluster 32
The addition of phytase to swine diets has generally increased P digestibility and consequently reduced fecal excretion of P. ... We conducted 2 studies with 2 treatments in young growing pigs (8 to 40 kg) to estimate potential contributions of phytase to ... Phytase inclusion in pig diets improves zinc status but its effect on ... ... Comparative effects of three phytases on the phosphorus and calcium use in ... ...
more infohttp://www.biomedsearch.com/cluster/23/Diet-and-Cholesterol/sub-32.html
NY I82324 - The tariff classification of Phytase-based enzyme preparations from Mexico - United States International Trade...  NY I82324 - The tariff classification of Phytase-based enzyme preparations from Mexico - United States International Trade...
RE: The tariff classification of Phytase-based enzyme preparations from Mexico. Dear Mr. Correia:. In your letter dated May 8, ... 6-phytase is found in both plants and animals, and hydrolyses excess dietary phytic acid. One of the preparations is composed ... The subject products consist of two enzyme preparations, each containing a genetically modified form of the enzyme, 6-phytase, ...
more infohttp://www.faqs.org/rulings/rulings2002NYI82324.html
EP2051590B1 - Enzyme granules for animal feed 
        - Google Patents  EP2051590B1 - Enzyme granules for animal feed - Google Patents
Other fungal phytases such as those disclosed in JP 11000164. (Penicillium phytase), or WO98/13480. (Monascus anka phytase); ... an allelic variant of the phytase of (i)-(ix);. *xiii. a fragment of the phytase of (i) - (ix) that retains phytase activity; ... FTU is for determining phytase activity in feed and premix.. Preferred examples of phytases are microbial phytases, such as ... Three different types of phytases are known: A so-called 3-phytase (alternative name 1-phytase; a myo-inositol hexaphosphate 3- ...
more infohttps://patents.google.com/patent/EP2051590B1/en
CN1742084B - Stabilization of granules 
        - Google Patents  CN1742084B - Stabilization of granules - Google Patents
... phytase (EC 3. I. 3 .-), such as 3-phytases (EC 3. I. 3. 8) and 6-phytase (EC 3. I. 3. 26); glycosidases (EC 3. 2, it falls ... 0190] 洗涤剂可以包含0-65%助洗剂或配合剂如沸石、二磷酸盐、三磷酸盐、膦酸盐、碳酸盐、柠檬酸盐、氨三乙酸、 乙二胺四乙酸、二亚乙基三胺五乙酸、烷基-或链烯基琥珀酸、可溶性硅酸盐或层状硅酸盐(如来自Hoechst的SKS-6)。 [0190] ... 1.39)、α-糊精内-1,6_α-葡糖苷酶(EC 3.2. I. 41)、蔗糖α -葡糖苷酶(EC 3.2. I. 48)、葡聚糖内-I,3-α-葡糖苷酶(EC 3.2.1.59)、葡聚糖1,4-0-葡糖苷酶(EC 3. 2. I.
more infohttps://patents.google.com/patent/CN1742084B/en
Frontiers | Insights into Broilers Gut Microbiota Fed with Phosphorus, Calcium, and Phytase Supplemented Diets | Microbiology  Frontiers | Insights into Broilers' Gut Microbiota Fed with Phosphorus, Calcium, and Phytase Supplemented Diets | Microbiology
Here, we aim to distinguish the effects of mineral P, Ca and phytase on the composition of microbial communities present in the ... Here, we aim to distinguish the effects of mineral P, Ca and phytase on the composition of microbial communities present in the ... Phytase supplementation in broiler diets is a common practice to improve phosphorus (P) availability and to reduce P loss by ... Phytase supplementation in broiler diets is a common practice to improve phosphorus (P) availability and to reduce P loss by ...
more infohttps://www.frontiersin.org/articles/10.3389/fmicb.2016.02033/full
ScopeMed.org - Deposit for Medical Articles  ScopeMed.org - Deposit for Medical Articles
Journal of Applied Pharmaceutical Science. 2015; 5(6): 034-049.. *Patterns of CD4+ T-lymphocytes and liver enzymes in adult HIV ... International Journal of Livestock Research. 2016; 6(8): 6-14.. *Bilateral blindness due to pterygium: a case report. Richard I ... Journal of Experimental and Integrative Medicine. 2016; 6(2): 66-70.. *How to Improve Performance in Sport - A Clinical ... Journal of Applied Pharmaceutical Science. 2014; 4(6): 074-078.. *Montelukast versus Long Acting Beta-2 Agonists as Add on ...
more infohttp://www.scopemed.org/?term=Patrick%20G%20Duffy&sarea=author&b1=Search+ScopeMed
AllAboutFeed - Adisseo introduces Rovabio® Max for monogastrics  AllAboutFeed - Adisseo introduces Rovabio® Max for monogastrics
This synergetic NSP enzyme and phytase combination offers maximum benefits by reducing animal fat or vegetable oil up to 10Kg/ ... The 6-phytase increases the availability of dietary phosphorous and is active over a greater pH range than other commercially ...
more infohttps://www.allaboutfeed.net/Home/General/2009/2/Adisseo-introduces-Rovabio-Max-for-monogastrics-AAF002824W/
Alergia e Transgênicos  Alergia e Transgênicos
6-Phytase [biosynthesis]. Minor Subject Heading(s):. 6-Phytase [biosynthesis] [genetics]; Food Hypersensitivity [prevention & ... EC 3.1.3.26 (6-Phytase); 0 (Herbicides); 0 (Metalloproteins); 0 (Plant Oils); 0 (Plant Proteins); 98726-08-0 (phytochelatin). ...
more infohttp://luizmeira.com/molecula.htm
EEA-Lex | European Free Trade Association  EEA-Lex | European Free Trade Association
Commission Regulation (EU) No 327/2010 of 21 April 2010 concerning the authorisation of a new use of 3-phytase as a feed ... April 2010 zur Zulassung einer neuen Verwendung von 3-Phytase als Futtermittelzusatzstoff für alle Vogelarten von geringerer ... Commission Directive 2010/6/EU of 9 February 2010 amending Annex I to Directive 2002/32/EC of the European Parliament and of ... Richtlinie 2010/6/EU der Kommission vom 9. Februar 2010 zur Änderung des Anhangs I der Richtlinie 2002/32/EG des Europäischen ...
more infohttps://www.efta.int/eea-lex?sort_bef_combine=search_api_relevance%20DESC&0=field_celex_type%3AD&1=field_adoption_date_in_the_eu%3A1995&sort_by=search_api_relevance&sort_order=DESC&f%5B0%5D=field_adoption_date_in_the_eu%3A2010&f%5B1%5D=field_annex_chapter%3A586&%3Bf%5B1%5D=field_celex_type%3AD&%3Bf%5B2%5D=field_adoption_date_in_the_eu%3A1995
China Phytases Market Research Report 2017 - RnR Market Research  China Phytases Market Research Report 2017 - RnR Market Research
China Phytases Market Research Report 2017 is a market research report available at US $3400 for a Single User PDF License from ... 1.2.2 China Phytases Sales (MT) Market Share by Type in 2016. 1.2.3 Granular Phytases. 1.2.4 Powder Phytases. 1.2.5 Liquid ... Table Phytases Sales (MT) by Application (2016-2022). Figure Product Picture of Phytases. Table China Phytases Sales (MT) and ... 1 Phytases Overview. 1.1 Product Overview and Scope of Phytases. 1.2 Classification of Phytases by Product Category. 1.2.1 ...
more infohttp://www.rnrmarketresearch.com/china-phytases-market-research-report-2017-market-report.html
EEA-Lex | European Free Trade Association  EEA-Lex | European Free Trade Association
98/2012 að því er varðar lágmarksinnihald blöndu með 6-fýtasa (EC 3.1.3.26), sem er framleiddur með Komagataella pastoris (DSM ... 98/2012 med omsyn til lågaste innhald av 6-fytase (EC 3.1.3.26) framstilt av Komagataella pastoris (DSM 23036) som ... Commission Implementing Regulation (EU) 2016/329 of 8 March 2016 concerning the authorisation of 6-phytase as a feed additive ... 98/2012 hinsichtlich des Mindestgehalts an der Zubereitung 6-Phytase (EC 3.1.3.26), gewonnen aus Komagataella pastoris (DSM ...
more infohttps://www.efta.int/eea-lex?sort_bef_combine=search_api_relevance%20DESC&%3Bamp%3B0=field_celex_type%3AL&%3Bamp%3Bsort_by=search_api_relevance&%3Bamp%3Bsort_order=DESC&%3Bamp%3Bf%5B0%5D=field_adoption_date_in_the_eu%3A2009&%3Bamp%3Bf%5B1%5D=field_celex_type%3AL&%3Bamp%3Bf%5B2%5D=field_protocol_tax_%3A706&%3Bf%5B0%5D=field_adoption_date_in_the_eu%3A1971&f%5B0%5D=field_adoption_date_in_the_eu%3A2016&f%5B1%5D=field_celex_type%3AR&f%5B2%5D=field_annex_chapter%3A586
4-phytase - Wikipedia  4-phytase - Wikipedia
Lim PE; Tate ME (1973). "The phytases. II. Properties of phytase fractions F 1 F 2 from wheat bran and the myoinositol ... In enzymology, a 4-phytase (EC 3.1.3.26) is an enzyme that catalyzes the chemical reaction myo-inositol hexakisphosphate + H2O ... Tomlinson RV; Ballou CE (1962). "Myoinositol polyphosphate intermediates in the dephosphorylation of phytic acid by phytase". ... phytase, and phytate 6-phosphatase. This enzyme participates in inositol phosphate metabolism. Johnson LF; Tate ME (1969). "The ...
more infohttps://en.wikipedia.org/wiki/4-phytase
  • Three barrows weighing 45.0 kg, fitted with simple T-cannulas in both the duodenum and terminal ileum, were assigned to diets in a 3횞3 Latin Square design experiment to determine the effects of two calcium levels (0.8% vs 0.4%) on phytase activity and nutrient balance in growing pigs. (ajas.info)
  • For the production of phytases, variety of natural and recombinant expression systems employing bacteria, yeast and fungi are exploited to enhance the overall productivity. (mnpublishers.com)
  • The global Phytases market is valued at XX million USD in 2016 and is expected to reach XX million USD by the end of 2022, growing at a CAGR of XX% between 2016 and 2022. (rnrmarketresearch.com)
  • Broilers were fed a control diet [0.43, 0.40, 0.36, or 0.32% nonphytate P (nPP)] in the starter, grower, finisher, and withdrawal periods, respectively, a low Ca and P (LCaP) diet with a 0.05% reduction in nPP in each period, and these 2 diets supplemented with phytase at 600 phytase units/kg (nPP and Ca were reduced by 0.094% in diets with phytase). (biomedsearch.com)
  • 2015b), supplementing low P and Ca diets with monocalcium phosphate in the presence of a practical level of a microbial 6-phytase led to decreased InsP6 and P disappearance up to the terminal ileum. (deepdyve.com)
  • In each experiment, two diets with or without a phytase super-dose (2,500 and 5,000 phytase units [FTU]/kg, respectively) were compared. (usda.gov)
  • In both experiments, there were no significant differences in total feed fed, gross yield, final fish weight, survival, or packed cell volume between fish fed diets with or without phytase. (usda.gov)
  • Here, we aim to distinguish the effects of mineral P, Ca, and phytase on the composition of microbial communities present in the content and the mucosa layer of the gastrointestinal tract (GIT) of broiler chickens. (frontiersin.org)
  • To address the issue of environmental emissions, we established transgenic pigs harboring a single-copy quad-cistronic transgene and simultaneously expressing three microbial enzymes, β-glucanase, xylanase, and phytase in the salivary glands. (elifesciences.org)
  • Additionally, all dephosphorylation steps from myo-inositol 1,2,3,4,5,6-hexakis (dihydrogen phosphate) (InsP6) to MI were investigated in the digesta of the terminal ileum. (deepdyve.com)
  • However, a very high dose of phytase (12,500 FTU/kg) diminished the negative effects of monocalcium phosphate. (deepdyve.com)
  • Phytase super-dosing did not have additional benefits beyond the standard phytase dose on growth, survival, feed conversion, or blood hematocrit, and also had no beneficial effects on water quality. (usda.gov)
  • The standard phytase dose of 500 phytase units/kg of feed remains the recommendation for feeds used for final grow-out of pond-raised hybrid catfish. (usda.gov)
  • Phytase super-dosing does not appear to have additional benefits beyond the standard phytase dose on production characteristics or packed cell volume of pond-raised hybrid catfish, and had no beneficial effects on water quality. (usda.gov)
  • It was found also that in contrast to rye breads supplemented with a standard 3-phytase A, breads baked with 6-phytase A had fat content lowered by more than fifty percent. (media.pl)
  • This report studies the Phytases development status and future trend in China, focuses on top players in China, also splits Phytases by type and by applications, to fully and deeply research and reveal the market general situation and future forecast. (rnrmarketresearch.com)
  • Scientists with Mississippi State University and the USDA-ARS Warmwater Aquaculture Research Unit conducted two pond studies in consecutive years to evaluate responses of hybrid catfish to phytase "super-dosing" of existing commercial catfish feeds. (usda.gov)
  • Two experiments were conducted in consecutive years to evaluate responses of hybrid catfish, male Ictalurus punctatus X female Ictalurus furcatus, to "super-dosing" of 6-phytase added to existing commercial catfish feeds. (usda.gov)
  • Relationship between Maternal Knowledge on Exclusive Breastfeeding and Breastfeeding Practices Among Mothers with Infants (0-6 Months) in Kibera Slums, Nairobi County, Kenya. (scopemed.org)
  • As compared to rats fed control breads, improved HDL/TC ratios were found in plasma of animals that consumed breads prepared with 3- or 6- phytase A, or with 6-phytase A and phytase B. Rats fed breads supplemented with 6-phytase A and phytase B had lowered contents of total lipids, triacyloglicerols, and total cholesterol in sera. (media.pl)