Nucleotidases: A class of enzymes that catalyze the conversion of a nucleotide and water to a nucleoside and orthophosphate. EC 3.1.3.-.5'-Nucleotidase: A glycoprotein enzyme present in various organs and in many cells. The enzyme catalyzes the hydrolysis of a 5'-ribonucleotide to a ribonucleoside and orthophosphate in the presence of water. It is cation-dependent and exists in a membrane-bound and soluble form. EC 188.8.131.52.Apyrase: A calcium-activated enzyme that catalyzes the hydrolysis of ATP to yield AMP and orthophosphate. It can also act on ADP and other nucleoside triphosphates and diphosphates. EC 184.108.40.206.Cytidine Monophosphate: Cytidine (dihydrogen phosphate). A cytosine nucleotide containing one phosphate group esterified to the sugar moiety in the 2', 3' or 5' position.Haloferax volcanii: A species of halophilic archaea found in the Dead Sea.Pyrimidine Nucleotides: Pyrimidines with a RIBOSE and phosphate attached that can polymerize to form DNA and RNA.Steatitis: A disease of cats and mink characterized by a marked inflammation of adipose tissue and the deposition of "ceroid" pigment in the interstices of the adipose cells. It is believed to be caused by feeding diets containing too much unsaturated fatty acid and too little vitamin E. (Merck Veterinary Manual, 5th ed; Stedman, 25th ed)Anemia, Hemolytic: A condition of inadequate circulating red blood cells (ANEMIA) or insufficient HEMOGLOBIN due to premature destruction of red blood cells (ERYTHROCYTES).Uridine Monophosphate: 5'-Uridylic acid. A uracil nucleotide containing one phosphate group esterified to the sugar moiety in the 2', 3' or 5' position.Adenosine Monophosphate: Adenine nucleotide containing one phosphate group esterified to the sugar moiety in the 2'-, 3'-, or 5'-position.Phosphoric Monoester Hydrolases: A group of hydrolases which catalyze the hydrolysis of monophosphoric esters with the production of one mole of orthophosphate. EC 3.1.3.Adenosine Triphosphatases: A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.Pyrophosphatases: A group of enzymes within the class EC 3.6.1.- that catalyze the hydrolysis of diphosphate bonds, chiefly in nucleoside di- and triphosphates. They may liberate either a mono- or diphosphate. EC 3.6.1.-.Adenosine: A nucleoside that is composed of ADENINE and D-RIBOSE. Adenosine or adenosine derivatives play many important biological roles in addition to being components of DNA and RNA. Adenosine itself is a neurotransmitter.Kinetics: The rate dynamics in chemical or physical systems.Adenosine Diphosphate: Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.Archaeal Proteins: Proteins found in any species of archaeon.Adenosine Triphosphate: An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Erythrocytes: Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.Nucleotides: The monomeric units from which DNA or RNA polymers are constructed. They consist of a purine or pyrimidine base, a pentose sugar, and a phosphate group. (From King & Stansfield, A Dictionary of Genetics, 4th ed)Proteasome Endopeptidase Complex: A large multisubunit complex that plays an important role in the degradation of most of the cytosolic and nuclear proteins in eukaryotic cells. It contains a 700-kDa catalytic sub-complex and two 700-kDa regulatory sub-complexes. The complex digests ubiquitinated proteins and protein activated via ornithine decarboxylase antizyme.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Molecular Weight: The sum of the weight of all the atoms in a molecule.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Other names in common use include phosphoadenylate 3'-nucleotidase, 3'-phosphoadenylylsulfate 3'-phosphatase, phosphoadenylate ... bisphosphate nucleotidase (EC 220.127.116.11) is an enzyme that catalyzes the chemical reaction adenosine 3',5'-bisphosphate + H2O ... Bisphosphate nucleotidase". J. Biol. Chem. 262 (21): 10044-7. PMID 3038862. Lik-Shing Tsang M, Schiff JA (1976). "Properties of ... 3'-nucleotidase, and 3'(2'),5'-bisphosphonucleoside 3'(2')-phosphohydrolase. This enzyme participates in sulfur metabolism. As ...
27 (5): 1489-1495. doi:10.1016/j.tiv.2013.03.002. PMID 23524101. Chou, Janice Y.; Robinson, J. C. (1977-01-01). "Induction of ... 56 (5): 326-329. Ninfa; Ballou; Benore (2010). Biochemistry and Biotechnology. USA: John Wiley & Sons, INC. pp. 229-230. ISBN ... 10 (5): e0124835. doi:10.1371/journal.pone.0124835. PMC 4422672 . PMID 25946026. CS1 maint: Uses authors parameter (link) Kay, ... doi:10.1186/s40697-015-0066-5. CS1 maint: Uses authors parameter (link) Mizumori, M., Ham, M., Guth, P. H., Engel, E., Kaunitz ...
... nucleotidase, thymidylate nucleotidase, deoxythymidylic 5'-nucleotidase, deoxythymidylate phosphohydrolase, and dTMPase. ... nucleotidase. Purification and properties of an enzyme found after infection of Bacillus subtilis with phage SP5C". J. Biol. ... In enzymology, a thymidylate 5'-phosphatase (EC 18.104.22.168) is an enzyme that catalyzes the chemical reaction thymidylate + H2O ... The systematic name of this enzyme class is thymidylate 5'-phosphohydrolase. Other names in common use include thymidylate 5'- ...
... nucleotidase, cytosolic IA is a protein that in humans is encoded by the NT5C1A gene. Cytosolic nucleotidases, such as NT5C1A, ... nucleotidase I: characterization and role in nucleoside analog resistance". J. Biol. Chem. 276 (13): 10498-504. doi:10.1074/jbc ... nucleotidase, cytosolic IA". Hunsucker SA, Spychala J, Mitchell BS (2001). "Human cytosolic 5'- ...
... pyrimidine nucleotidases. cN-I is a cytosolic nucleotidase(cN) characterized by its affinity toward AMP as its substrate. cN-II ... nucleotidase - NT5C, NT5C1A, NT5C1B, NT5C2, NT5C3 EC 22.214.171.124: 3'-nucleotidase - NT3 5'-Nucleotidases cleave off the phosphate ... nucleotidases as regulators of nucleotide and drug metabolism". Pharmacol. Ther. 107, 1-30. Nucleotidases at the US National ... A nucleotidase is a hydrolytic enzyme that catalyzes the hydrolysis of a nucleotide into a nucleoside and a phosphate. A ...
Herbert Zimmermann (neuroscientist)
... nucleotidase as well as of a number of the nucleoside triphosphate and diphosphate-hydrolyzing enzymes of the family of the ... nucleotidase from Torpedo electric organ". Biochem. J. 245: 805-810. Volknandt, W.; Vogel, M.; Pevsner, J.; Misumi, Y.; Ikehara ... nucleotidase from the electric ray electric lobe. Primary structure and relation to mammalian and procaryotic enzymes". Biochem ... "Cellular function and molecular structure of ecto-nucleotidases". Purinergic Signal. 8 (3): 437-502. doi:10.1007/s11302-012- ...
... nucleotidase, ecto (CD73)". St Hilaire C, Ziegler SG, Markello TC, Brusco A, Groden C, Gill F, Carlson-Donohoe H, Lederman RJ, ... nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. CD73 commonly ... nucleotidase". Life Sciences. 62 (25): 2257-66. doi:10.1016/S0024-3205(98)00206-9. PMID 9651114. NT5E protein, human at the US ... nucleotidase (CD73)". Tissue Antigens. 35 (1): 9-19. doi:10.1111/j.1399-0039.1990.tb01750.x. PMID 2137649. Klemens MR, Sherman ...
... nucleotidase, and phosphodiesterase. The skin of the bluespotted stingray is often used for drums such as on the Arab and ...
... nucleotidase (CD73) and A2B adenosine receptors. Circulation. 2007 Mar 27;115(12):1581-90. PMID 17353435 Grenz A, Osswald H, ... 2008 Jun 24;5(6):e137. PMID 18578565 Eckle T, Grenz A, Laucher S, Eltzschig HK. A2B adenosine receptor signaling attenuates ...
... nucleotidase family". Exp. Parasitol. 122 (4): 318-27. doi:10.1016/j.exppara.2009.04.007. PMID 19393241. http://www.moh.gov.sa/ ... 13 (1): 153-5. doi:10.3201/eid1301.061094. PMC 2725816 . PMID 17370534. Rico-Hesse, Rebeca; Dodd, Kimberly A.; Bird, Brian H.; ... 5 (10): e1352. doi:10.1371/journal.pntd.0001352. PMID 21991403. http://www.jsava.co.za/index.php/jsava/article/download/1255/ ... 19 (5): 325-332. doi:10.1002/psc.2505. http://www.merckvetmanual.com/mvm/integumentary_system/ticks/ornithodoros_spp.html http ...
A nucleotidase is an enzyme that catalyzes the hydrolysis of a nucleotide, forming a nucleoside and a phosphate ion. ... Inside the cell, nucleotidases may help to maintain energy levels under stress conditions. A cell deprived of oxygen and ... Some nucleotidases function outside the cell, creating nucleosides that can be transported into the cell and used to regenerate ... Nucleotidases are essential for cellular homeostasis, because they are partially responsible for maintaining a balanced ratio ...
... nucleotidase 2 (dNT-2), is an enzyme that in humans is encoded by the NT5M gene. This gene encodes a 5' nucleotidase that ... nucleotidase, mitochondrial". Rampazzo C, Gallinaro L, Milanesi E, Frigimelica E, Reichard P, Bianchi V (Jul 2000). "A ... nucleotidases identified in humans, all of which differ in tissue specificity, subcellular location, primary structure and ... nucleotidase, mitochondrial, also known as 5'(3')-deoxyribonucleotidase, mitochondrial (mdN) or deoxy-5'- ...
... nucleotidase activity in workers exposed to lead, mercury or cadmium". Int Arch Occup Environ Health. 55 (3): 247-52. doi: ... nucleotidase, important for the correct metabolism of DNA and can therefore cause fetal damage. Chemistry portal Pigments ... 5 (3): 242-242. doi:10.1021/je60007a002. Pauley, J. L.; M. K. Testerman (1954). "Basic Salts of Lead Nitrate Formed in Aqueous ... 5 (3): 242. doi:10.1021/je60007a002. "Historical development of titanium dioxide". Millennium Inorganic Chemicals. Archived ...
... nucleotidase activity via Ca2+-dependent, annexin 2-mediated membrane rearrangement?". Biochemical Society Transactions. 34 (Pt ... 33 (5): 1223-8. doi:10.1021/bi00171a023. PMID 8110754. Wright JF, Kurosky A, Wasi S (Feb 1994). "An endothelial cell-surface ... Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". The Journal of Biological Chemistry. 277 ( ... doi:10.1016/S0300-9084(02)00027-5. PMID 12628297. Kirshner J, Schumann D, Shively JE (Dec 2003). "CEACAM1, a cell-cell adhesion ...
Laminin, alpha 1
... nucleotidase) in 165 glioblastomas by immunohistochemistry and electronmicroscopic histochemistry". Anticancer Res. 19 (3A): ... 272 (5): 2814-20. doi:10.1074/jbc.272.5.2814. ISSN 0021-9258. PMID 9006922. Talts, J F; Sasaki T; Miosge N; Göhring W; Mann K; ... 5 (4): 932-5. doi:10.1016/0888-7543(89)90136-5. PMID 2591971. "Entrez Gene: LAMA1 laminin, alpha 1". Utani, A; Nomizu M; Yamada ... Utani A, Nomizu M, Yamada Y (1997). "Fibulin-2 binds to the short arms of laminin-5 and laminin-1 via conserved amino acid ...
... bisphosphate nucleotidase to give adenosine monophosphate, which can then be recycled into adenosine triphosphate. Adenine ... Adenosine 3',5'-bisphosphate is produced as a product of sulfotransferase enzymes from the donation of a sulfate group from the ... Adenosine 3',5'-bisphosphate is a form of an adenosine nucleotide with two phosphate groups attached to different carbons in ... This is distinct from adenosine diphosphate, where the two phosphate groups are attached in a chain to the 5' carbon atom in ...
1995). "Homogeneous pyrimidine nucleotidase from human erythrocytes: enzymic and molecular properties". Biochem. J. 304 (Pt 3 ... 1998). "Pyrimidine nucleotidases from human erythrocyte possess phosphotransferase activities specific for pyrimidine ... nucleotidase (PN-I or P5'NI), and p56, is an enzyme that in humans is encoded by the NT5C3, or NT5C3A, gene on chromosome 7. ... nucleotidase family and one of the five cytosolic members identified in humans. NTC53 catalyzes the dephosphorylation of the ...
... nucleotidase". Biochim. Biophys. Acta. 33 (1): 101-5. doi:10.1016/0006-3002(59)90502-5. PMID 13651188. Molecular and Cellular ... In enzymology, a 5'-acylphosphoadenosine hydrolase (EC 126.96.36.199) is an enzyme that catalyzes the chemical reaction 5'- ... The systematic name of this enzyme class is 5'-acylphosphoadenosine acylhydrolase. This enzyme is also called 5- ... KELLERMAN GM (1959). "Isolation and characteristics of the enzyme acyl 5'- ...
Of the different forms of nucleotidases characterized, this enzyme is unique in its preference for 5'-dNTP. It may be one of ... This gene encodes a nucleotidase that catalyzes the dephosphorylation of the 5' deoxyribonucleotides (dNTP) and 2'(3')-dNTP and ... nucleotidase mRNA levels in blast cells are a prognostic factor in acute myeloid leukemia patients treated with cytarabine". ... nucleotidase mRNA levels in blast cells are a prognostic factor in acute myeloid leukemia patients treated with cytarabine". ...
SurE, survival protein E
... nucleotidases (EC), 3'-nucleotidases (EC) and exopolyphosphatases (EC). The gene, surE, is part of a bicistronic operon found ... 326 (5): 1559-75. doi:10.1016/S0022-2836(03)00056-1. PMID 12595266. This article incorporates text from the public domain Pfam ... This domain is found in acid phosphatases (EC), 5'- ...
5'-nucleotidase - Wikipedia
... nucleotidase, thimidine monophosphate nucleotidase, 5'-AMPase, 5'-AMP nucleotidase, AMP phosphohydrolase, IMP 5'-nucleotidase ... Reis, Julian (1934). "Nucleotidase and its relation to the deamination of nucleotides in the heart and the muscles". Bulletin ... Nucleotidase Levels Blood Test. Ecto-5'Nucleotidase is considered a maturation marker for T cells and B cells. This is due to ... nucleotidase and has an extracellular location. The two cytosolic forms of the enzyme have similar characteristics, but can be ...https://en.wikipedia.org/wiki/5'-nucleotidase
Pyrimidine-5-Nucleotidase Deficiency (Pyrimidine 5-Nucleotidase Deficiency): Symptoms, Diagnosis and Treatment - Symptoma®
Pyrimidine-5-Nucleotidase Deficiency (Pyrimidine 5-Nucleotidase Deficiency): Read more about Symptoms, Diagnosis, Treatment, ... nucleotidase (P-5'-N) deficiency, presented within a few days of each other with a febrile illness and pancytopenia.[ncbi.nlm. ... nucleotidase deficiency diagnosed in the Scandinavian countries.[ncbi.nlm.nih.gov] ... We present 2 Norwegian siblings who we believe to be the first cases of pyrimidine 5'- ...https://www.symptoma.com/en/info/pyrimidine-5-nucleotidase-deficiency
Cobalt activation of Escherichia coli 5'-nucleotidase is due to zinc ion displacement at only one of two metal-ion-binding...
... nucleotidase is due to zinc ion displacement at only one of two metal-ion-binding sites. Lyle McMILLEN, Ifor R. BEACHAM, Dennis ... nucleotidase in which a Co2+ ion may displace the Zn2+ ion at only one of two metal-ion-binding sites; the other metal-ion- ... nucleotidase (Asp41, His43, Asp84, His117, Glu118, His217 and His252) were selected for modification using site-directed ... nucleotidase activity is stimulated 30- to 50-fold in vitro by the addition of Co2+. Seven residues from conserved sequence ...http://www.biochemj.org/content/372/2/625
Ecto-5′ -nucleotidase deficiency exacerbates pressure-overload- induced left ventricular hypertrophy and dysfunction | 胡新立
... nucleotidase deficiency exacerbates pressure-overload- induced left ventricular hypertrophy and dysfunction. Hypertension [ ... nucleotidase deficiency exacerbates pressure-overload- induced left ventricular hypertrophy and dysfunction ... nucleotidase deficiency exacerbates pressure-overload- induced left ventricular hypertrophy and dysfunction. Hypertension [ ... Xu X, Fassett J, Hu X, Zhu G, Lu Z, Li Y, Schnermann J, Bache RJ, Chen Y. Ecto-5′ - ...http://scholar.pku.edu.cn/lxhu/publications/ecto-5%E2%80%B2-nucleotidase-deficiency-exacerbates-pressure-overload-induced-left
Pyrimidine 5'-nucleotidase definition | Drugs.com
... nucleotidase. Provided by Stedman's medical dictionary and Drugs.com. Includes medical terms and definitions. ... nucleotidase. Definition: an enzyme that catalyzes the hydrolysis of a pyrimidine-nucleoside 5'-monophosphate to produce ...https://www.drugs.com/dict/pyrimidine-5-nucleotidase.html
Ecto-5′-Nucleotidase as a Leukemia Marker | SpringerLink
Nucleotidase-positive and-negative subpopulations, J.Clin.Invest. 56: 1324-1327 (1975).PubMedCrossRefGoogle Scholar ... Nucleotidase as a Leukemia Marker. In: De Bruyn C.H.M.M., Simmonds H.A., Müller M.M. (eds) Purine Metabolism in Man-IV. ... Nucleotidase (5′-N) as an ectoenzyme has the double advantage of being testable by an ordinary enzyme assay on intact cells and ... We have characterized leukemic cells using 5′-N not as an isolated marker but rather within a battery of other markers ...https://link.springer.com/chapter/10.1007/978-1-4757-0390-0_48
RCSB PDB - Gene View - NT5E - 5'-nucleotidase ecto
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.http://www.rcsb.org/pdb/gene/NT5E
Ecto-Enzyme of Granulocytes: 5'-Nucleotidase | Science
... nucleotidase of guinea pig polymorphonuclear leukocytes is localized exclusively on the plasma membrane of intact cells. The ...http://science.sciencemag.org/content/183/4129/1096
Nt5e 5' nucleotidase, ecto [Rattus norvegicus (Norway rat)] - Gene - NCBI
... nucleotidase, ectoprovided by RGD. Primary source. RGD:61956 See related. Ensembl:ENSRNOG00000011071 Gene type. protein coding ... nucleotidase family [Nucleotide transport and metabolism, Defense mechanisms]. cd07409. Location:31 → 312. MPP_CD73_N; CD73 ... nucleotidase. Names. 5'-NT. ecto-5'-nucleotidase. NP_067587.2. *EC 188.8.131.52 ... nucleotidase and related proteins, N-terminal metallophosphatase domain. pfam02872. Location:341 → 509. 5_nucleotid_C; 5'- ...https://www.ncbi.nlm.nih.gov/gene/58813
Domain architectures: 5'-Nucleotidase, C-terminal domain superfamily (IPR036907) | InterPro | EMBL-EBI
Nucleotidase, C-terminal domain superfamily (IPR036907). Showing 1 to 20 of 355 results ...http://www.ebi.ac.uk/interpro/entry/IPR036907/domain-organisations
5'-Nucleotidase/CD73 Antibody (AD2) (NBP2-48480): Novus Biologicals
Nucleotidase/CD73 Antibody (AD2) cited in 15 publications. Validated: Flow, Flow-CS, IHC-Fr. Tested Reactivity: Human. ... Nucleotidase/CD73.. Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target. By Yoskaly Lazo-Fernandez, ... Nucleotidase/CD73 Antibody (NBP2-48480). (Showing 1 - 1 of 1 FAQs).. * We are interested in CD73 antibodies that could react ... nucleotidase (CD73) in normal mammary gland and in breast carcinoma. Br J Cancer. 1991 Jan 1 [PMID: 1989648] (IHC-Fr, Human). ...https://www.novusbio.com/products/5-nucleotidase-cd73-antibody-ad2_nbp2-48480
Early changes in 5′-nucleotidase activity in heriditary degeneration of the retina | SpringerLink
... nucleotidase phosphodiesterase hereditary degeneration of the retina Laboratory of Biochemical Bases of Reception, I. M. ... nucleotidase activity in heriditary degeneration of the retina. ... 5.. A. L. Berman, A. M. Azimova, and F. G. Gribakin, Vision Res ...https://link.springer.com/article/10.1007/BF00829532
5'-Nucleotidase/CD73 Antibody (45M3B4) [HRP] (NBP2-25235H): Novus Biologicals
Nucleotidase/CD73 Antibody (45M3B4) [HRP]. Validated: WB. Tested Reactivity: Human, Mouse, Rat, and more. 100% Guaranteed. ... Nucleotidase/CD73 Antibody (45M3B4) [HRP] Summary. Immunogen. Within the range of amino acids 169-324 of human CD73 were used ... Nucleotidase/CD73.. Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target. By Yoskaly Lazo-Fernandez, ... Nucleotidase/CD73 Antibody (NBP2-25235H). (Showing 1 - 1 of 1 FAQ).. * We are interested in CD73 antibodies that could react ...https://www.novusbio.com/products/5-nucleotidase-cd73-antibody-45m3b4_nbp2-25235h
Structural Studies of Human 5'-Nucleotidases - OpenThesis
Keywords:TECHNOLOGY; Bioengineering; Structural biochemistry; Nucleotidases; nucleotide; nucleoside; nucleoside analogue; ... nucleotidase II were used to characterize the structural basis for their substrate specificity in high detail.Based on ... nucleotidase II, a mechanism for the allosteric activation of this enzyme was presented. In this mechanism, the activator ... Nucleotidases (5'NTs) are catabolic enzymes of the nucleotide metabolism. They catalyze dephosphorylation of deoxyribo- and ...http://www.openthesis.org/documents/Structural-Studies-Human-5-Nucleotidases-436373.html
Crucial Role for Ecto-5′-Nucleotidase (CD73) in Vascular Leakage during Hypoxia | JEM
One factor may be the level of AMP-hydrolyzing activity attributed to enzymes other than CD73 (i.e., other nucleotidases and/or ... Nucleotidase (CD73) in Vascular Leakage during Hypoxia. Linda F. Thompson, Holger K. Eltzschig, Juan C. Ibla, C. Justin Van De ... nucleotidase activity and cell surface expression in endothelial cells: role of membrane lipids. Circ. Res. 92:848-855. ... Nucleotidase from smooth muscle of small intestine and from brain. Inhibition by nucleotides. Biochemistry. 14:2362-2366. ...http://jem.rupress.org/content/200/11/1395
Ecto-5'-nucleotidase (CD73) regulation by hypoxia-inducible factor-1 mediates permeability changes in intestinal epithelia. -...
... nucleotidase (CD73) regulation by hypoxia-inducible factor-1 mediates permeability changes in intestinal epithelia.. ... nucleotidase (CD73) regulation by hypoxia-inducible factor-1 mediates permeability changes in intestinal epithelia ... nucleotidase (CD73) regulation by hypoxia-inducible factor-1 mediates permeability changes in intestinal epithelia ... nucleotidase (CD73) regulation by hypoxia-inducible factor-1 mediates permeability changes in intestinal epithelia ...https://www.ncbi.nlm.nih.gov/pubmed/12370277?dopt=Abstract
5'-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain family
... nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain family. Additional information, provided for both this family and ... nucleotidase activity. 0. 2.376. --. DIRECT. Molecular Function (MF). phosphoric ester hydrolase activity. 0. 1.406. --. DIRECT ... 3'-nucleotidase. 0.0000001668. 2.264. --. DIRECT. Enzyme Commission (EC). Acting on ester bonds. 0.0000000001597. 0.5107. Least ... nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain family. SCOP classification Root: SCOP hierarchy in SUPERFAMILY [. 0 ...http://supfam.org/SUPERFAMILY/cgi-bin/scop.cgi?sunid=55817
Purification, characterization and cellular localization of 5′-nucleotidase from Torpedo electric organ | Biochemical Journal |...
... nucleotidase from Torpedo electric organ E J M Grondal E J M Grondal ... Nucleotidase was isolated from the electric organ of the electric ray Torpedo marmorata after solubilization in Triton X-100 ... nucleotidase from electric-ray ( Torpedo marmorata ) electric organ and bovine cerebral cortex is derived from the glycosyl- ... nucleotidase from Torpedo marmorata (electric ray) electric organ Biochem J (August, 1991) ...https://portlandpress.com/biochemj/article/245/3/805/23537/Purification-characterization-and-cellular
Pyrimidine 5'-nucleotidase | definition of pyrimidine 5'-nucleotidase by Medical dictionary
... nucleotidase explanation free. What is pyrimidine 5'-nucleotidase? Meaning of pyrimidine 5'-nucleotidase medical term. What ... Looking for online definition of pyrimidine 5'-nucleotidase in the Medical Dictionary? pyrimidine 5'- ... nucleotidase. py·rim·i·dine 5'-nu·cle·o·ti·dase. an enzyme that catalyzes the hydrolysis of a pyrimidine-nucleoside 5'- ... nucleotidase deficiency using [sup.. [sup.1]H-NMR spectroscopy of body fluids: inborn errors of purine and pyrimidine ...https://medical-dictionary.thefreedictionary.com/pyrimidine+5%27-nucleotidase
Normal levels of 5'-nucleotidase activity in lymphocytes from patients with X-linked agammaglobulinemia | Science
... nucleotidase activity in lymphocytes from patients with X-linked agammaglobulinemia ... nucleotidase activity in lymphocytes from patients with X-linked agammaglobulinemia ... nucleotidase activity in lymphocytes from patients with X-linked agammaglobulinemia ... nucleotidase activity in lymphocytes from patients with X-linked agammaglobulinemia ...http://science.sciencemag.org/content/205/4405/520
- We present 2 Norwegian siblings who we believe to be the first cases of pyrimidine 5'-nucleotidase deficiency diagnosed in the Scandinavian countries. (symptoma.com)
- Abstract Two siblings with chronic hemolytic anemia due to red cell pyrimidine-5'-nucleotidase (P-5'-N) deficiency, presented within a few days of each other with a febrile illness and pancytopenia. (symptoma.com)
- Orthologues of Streptococcus pyogenes nuclease A (SpnA) and Streptococcal 5′-nucleotidase A... Soh, Kar Yan;Loh, Jacelyn Mei San;Proft, Thomas 2018-04-05 00:00:00 Abstract Streptococcus pyogenes nuclease A (SpnA) and streptococcal 5′ nucleosidase A (S5nA) are two recently described virulence factors from the human pathogen S. pyogenes. (deepdyve.com)
- Their dephosphorylation by 5'NTs might be one of the mechanisms behind the resistance experienced by patients towards such drugs.This thesis describes structure-function studies on four of the seven known human 5'-NTs. (openthesis.org)
- However, cytoplasmic 5′-nucleotidases catalyze dephosphorylation of ara-CMP, thereby reducing the amount of ara-C for conversion to ara-CTP ( Amici and Magni, 2002 ). (aspetjournals.org)
- also known as cN-II and high K m 5′-nucleotidase) and cytoplasmic 5′-nucleotidase III (NT5C3) are involved in the final step of dephosphorylation before export of nucleosides out of the cell. (aspetjournals.org)
- The similarity in the coding region to that of the human 5ʹ-nucleotidase cDNA sequence [Oka, Matsumoto, Hosokawa and Inoue (1994) Biochem. (biochemj.org)
- Two full-length putative 5'-nucleotidase isoforms were identified that shared significant sequence identity and similarity to a 5'-nucleotidase from R. (B.) microplus and putative apyrases from I. scapularis and R. appendiculatus. (up.ac.za)
- Seven residues from conserved sequence motifs implicated in the catalytic and metal-ion-binding sites of E. coli 5′-nucleotidase (Asp 41 , His 43 , Asp 84 , His 117 , Glu 118 , His 217 and His 252 ) were selected for modification using site-directed mutagenesis of the cloned ushA gene. (biochemj.org)
- Inactive variants of the mitochondrial and cytosolic deoxynucleotidases and the cytosolic 5'-nucleotidase II were used to characterize the structural basis for their substrate specificity in high detail.Based on structures of the apoprotein and activator/activator+substrate complexes of cytosolic 5'-nucleotidase II, a mechanism for the allosteric activation of this enzyme was presented. (openthesis.org)
- Nucleotidase with a broad substrate specificity as it can dephosphorylate various ribo- and deoxyribonucleoside 5'-monophosphates and ribonucleoside 3'-monophosphates with highest affinity to 3'-AMP. (uniprot.org)
- Objectives The diagnosis of inclusion body myositis (IBM) can be challenging as it can be difficult to clinically distinguish from other forms of myositis, particularly polymyositis (PM). Recent studies have shown frequent presence of autoantibodies directed against cytosolic 5′-nucleotidase 1A (cN-1A) in patients with IBM. (bmj.com)
- Furthermore, most arthropod apyrases are ascribed to the 5'-nucleotidase/CD73 enzyme family. (up.ac.za)
- The salivary apyrase from Ornithodoros savignyi has not been characterised to a specific enzyme family and the presence of 5'-nucleotidase homologs have not been demonstrated. (up.ac.za)
- Phylogenetic analysis of the 5'-nucleotidase family revealed that the O. savignyi 5'-nucleotidase isoforms claded monophyletically with the putative apyrases from I. scapularis and R. appendiculatus, excluding the 5'-nucleotidase from R. (B.) microplus. (up.ac.za)
- The latter implied that the native apyrase may be a member of the 5'-nucleotidase enzyme family. (up.ac.za)
- GAS produces a plethora of virulence factors that facilitate colonization, bacterial spreading and immune evasion (5-7). (deepdyve.com)
- We have recently characterized two novel cell wall-anchored immune evasion factors of GAS, the Streptococcus pyogenes nuclease A (SpnA) and Streptococcal 5′-nucleotidase A (S5nA) (8, 9). (deepdyve.com)