Nucleotidases: A class of enzymes that catalyze the conversion of a nucleotide and water to a nucleoside and orthophosphate. EC 3.1.3.-.5'-Nucleotidase: A glycoprotein enzyme present in various organs and in many cells. The enzyme catalyzes the hydrolysis of a 5'-ribonucleotide to a ribonucleoside and orthophosphate in the presence of water. It is cation-dependent and exists in a membrane-bound and soluble form. EC 3.1.3.5.Apyrase: A calcium-activated enzyme that catalyzes the hydrolysis of ATP to yield AMP and orthophosphate. It can also act on ADP and other nucleoside triphosphates and diphosphates. EC 3.6.1.5.Cytidine Monophosphate: Cytidine (dihydrogen phosphate). A cytosine nucleotide containing one phosphate group esterified to the sugar moiety in the 2', 3' or 5' position.Haloferax volcanii: A species of halophilic archaea found in the Dead Sea.Pyrimidine Nucleotides: Pyrimidines with a RIBOSE and phosphate attached that can polymerize to form DNA and RNA.Steatitis: A disease of cats and mink characterized by a marked inflammation of adipose tissue and the deposition of "ceroid" pigment in the interstices of the adipose cells. It is believed to be caused by feeding diets containing too much unsaturated fatty acid and too little vitamin E. (Merck Veterinary Manual, 5th ed; Stedman, 25th ed)Anemia, Hemolytic: A condition of inadequate circulating red blood cells (ANEMIA) or insufficient HEMOGLOBIN due to premature destruction of red blood cells (ERYTHROCYTES).Uridine Monophosphate: 5'-Uridylic acid. A uracil nucleotide containing one phosphate group esterified to the sugar moiety in the 2', 3' or 5' position.Adenosine Monophosphate: Adenine nucleotide containing one phosphate group esterified to the sugar moiety in the 2'-, 3'-, or 5'-position.Phosphoric Monoester Hydrolases: A group of hydrolases which catalyze the hydrolysis of monophosphoric esters with the production of one mole of orthophosphate. EC 3.1.3.Adenosine Triphosphatases: A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.Pyrophosphatases: A group of enzymes within the class EC 3.6.1.- that catalyze the hydrolysis of diphosphate bonds, chiefly in nucleoside di- and triphosphates. They may liberate either a mono- or diphosphate. EC 3.6.1.-.Adenosine: A nucleoside that is composed of ADENINE and D-RIBOSE. Adenosine or adenosine derivatives play many important biological roles in addition to being components of DNA and RNA. Adenosine itself is a neurotransmitter.Kinetics: The rate dynamics in chemical or physical systems.Adenosine Diphosphate: Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.Archaeal Proteins: Proteins found in any species of archaeon.Adenosine Triphosphate: An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Erythrocytes: Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.Nucleotides: The monomeric units from which DNA or RNA polymers are constructed. They consist of a purine or pyrimidine base, a pentose sugar, and a phosphate group. (From King & Stansfield, A Dictionary of Genetics, 4th ed)Proteasome Endopeptidase Complex: A large multisubunit complex that plays an important role in the degradation of most of the cytosolic and nuclear proteins in eukaryotic cells. It contains a 700-kDa catalytic sub-complex and two 700-kDa regulatory sub-complexes. The complex digests ubiquitinated proteins and protein activated via ornithine decarboxylase antizyme.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Molecular Weight: The sum of the weight of all the atoms in a molecule.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Software: Sequential operating programs and data which instruct the functioning of a digital computer.ArchivesEncyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Vertebrates: Animals having a vertebral column, members of the phylum Chordata, subphylum Craniata comprising mammals, birds, reptiles, amphibians, and fishes.Phosphodiesterase I: A phosphoric diester hydrolase that removes 5'-nucleotides from the 3'-hydroxy termini of 3'-hydroxy-terminated OLIGONUCLEOTIDES. It has low activity towards POLYNUCLEOTIDES and the presence of 3'-phosphate terminus on the substrate may inhibit hydrolysis.Snake Venoms: Solutions or mixtures of toxic and nontoxic substances elaborated by snake (Ophidia) salivary glands for the purpose of killing prey or disabling predators and delivered by grooved or hollow fangs. They usually contain enzymes, toxins, and other factors.USSRInfluenza A Virus, H2N2 Subtype: A subtype of INFLUENZA A VIRUS comprised of the surface proteins hemagglutinin 2 and neuraminidase 2. The H2N2 subtype was responsible for the Asian flu pandemic of 1957.Retina: The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent.RussiaBiotin: A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.Glycosylphosphatidylinositols: Compounds containing carbohydrate or glycosyl groups linked to phosphatidylinositols. They anchor GPI-LINKED PROTEINS or polysaccharides to cell membranes.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Antigens, CD3: Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).Uridine Diphosphate SugarsMarkov Chains: A stochastic process such that the conditional probability distribution for a state at any future instant, given the present state, is unaffected by any additional knowledge of the past history of the system.Protein Structure, Tertiary: The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.Galactosephosphates: Phosphoric acid esters of galactose.
... nucleotidase. The nucleoside, adenosine, is then deaminated and hydrolyzed to form hypoxanthine via adenosine deaminase and ... Nelson, David L.; Cox, Michael M.; Lehninger, Albert L. (2008). Lehninger's Principles of Biochemistry (5 ed.). Macmillan. ISBN ... First, the nucleotide will lose its phosphate through 5'- ... 5]. Deficiencies of enzymes involved in pyrimidine synthesis ...
... nucleotidase (cN1A; NT5C1A) have been strongly associated with the condition. In the clinical context of a classic history and ... 35 (5): 549-561. doi:10.1002/mus.20766. PMID 17366591. Karpati G, O'Ferrall EK (Jan 2009). "Sporadic inclusion body myositis: ... Patients may become unable to perform activities of daily living and most require assistive devices within 5 to 10 years of ...
... nucleotidase, cytosolic IA is a protein that in humans is encoded by the NT5C1A gene. Cytosolic nucleotidases, such as NT5C1A, ... nucleotidase I: characterization and role in nucleoside analog resistance". J. Biol. Chem. 276 (13): 10498-504. doi:10.1074/jbc ... nucleotidase, cytosolic IA". Hunsucker SA, Spychala J, Mitchell BS (2001). "Human cytosolic 5'- ...
... nucleotidase 2 (dNT-2), is an enzyme that in humans is encoded by the NT5M gene. This gene encodes a 5' nucleotidase that ... nucleotidase, mitochondrial". Rampazzo C, Gallinaro L, Milanesi E, Frigimelica E, Reichard P, Bianchi V (Jul 2000). "A ... nucleotidases identified in humans, all of which differ in tissue specificity, subcellular location, primary structure and ... nucleotidase, mitochondrial, also known as 5'(3')-deoxyribonucleotidase, mitochondrial (mdN) or deoxy-5'- ...
... nucleotidase, ecto (CD73)". St Hilaire C, Ziegler SG, Markello TC, Brusco A, Groden C, Gill F, Carlson-Donohoe H, Lederman RJ, ... nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. CD73 commonly ... nucleotidase". Life Sciences. 62 (25): 2257-66. doi:10.1016/S0024-3205(98)00206-9. PMID 9651114. NT5E protein, human at the US ... nucleotidase (CD73)". Tissue Antigens. 35 (1): 9-19. doi:10.1111/j.1399-0039.1990.tb01750.x. PMID 2137649. Klemens MR, Sherman ...
Of the different forms of nucleotidases characterized, this enzyme is unique in its preference for 5'-dNTP. It may be one of ... This gene encodes a nucleotidase that catalyzes the dephosphorylation of the 5' deoxyribonucleotides (dNTP) and 2'(3')-dNTP and ... nucleotidase mRNA levels in blast cells are a prognostic factor in acute myeloid leukemia patients treated with cytarabine". ... nucleotidase mRNA levels in blast cells are a prognostic factor in acute myeloid leukemia patients treated with cytarabine". ...
... nucleotidase as well as of a number of the nucleoside triphosphate and diphosphate-hydrolyzing enzymes of the family of the ... nucleotidase from Torpedo electric organ". Biochem. J. 245: 805-810. Volknandt, W.; Vogel, M.; Pevsner, J.; Misumi, Y.; Ikehara ... nucleotidase from the electric ray electric lobe. Primary structure and relation to mammalian and procaryotic enzymes". Biochem ... "Cellular function and molecular structure of ecto-nucleotidases". Purinergic Signal. 8 (3): 437-502. doi:10.1007/s11302-012- ...
... nucleotidase, and phosphodiesterase. The skin of the bluespotted stingray is often used for drums such as on the Arab and ...
... nucleotidase (CD73) and A2B adenosine receptors. Circulation. 2007 Mar 27;115(12):1581-90. PMID 17353435 Grenz A, Osswald H, ... 2008 Jun 24;5(6):e137. PMID 18578565 Eckle T, Grenz A, Laucher S, Eltzschig HK. A2B adenosine receptor signaling attenuates ...
... nucleotidase family". Exp. Parasitol. 122 (4): 318-27. doi:10.1016/j.exppara.2009.04.007. PMID 19393241. http://www.moh.gov.sa/ ... 13 (1): 153-5. doi:10.3201/eid1301.061094. PMC 2725816 . PMID 17370534. Rico-Hesse, Rebeca; Dodd, Kimberly A.; Bird, Brian H.; ... 5 (10): e1352. doi:10.1371/journal.pntd.0001352. PMID 21991403. http://www.jsava.co.za/index.php/jsava/article/download/1255/ ... 19 (5): 325-332. doi:10.1002/psc.2505. http://www.merckvetmanual.com/mvm/integumentary_system/ticks/ornithodoros_spp.html http ...
... nucleotidase. Adenosine constricts the afferent arteriole by binding with high affinity to the A1 receptors a Gi/Go. Adenosine ... nucleotidase (CD73) (Le Hir and Kaissling, 1993). This enzyme catalyzes the dephosphorylation of 5′-AMP or 5′-IMP to adenosine ... nucleotidase (Thomson et al., 2000). Extracellular adenosine activates A1 receptors on vascular afferent arteriolar smooth ... 299: F310-5. doi:10.1152/ajprenal.00149.2010. PMC 2928524 . PMID 20462966. Ortiz-Capisano, M. Cecilia; Atchison, Douglas K; ...
1995). "Homogeneous pyrimidine nucleotidase from human erythrocytes: enzymic and molecular properties". Biochem. J. 304 (Pt 3 ... 1998). "Pyrimidine nucleotidases from human erythrocyte possess phosphotransferase activities specific for pyrimidine ... nucleotidase (PN-I or P5'NI), and p56, is an enzyme that in humans is encoded by the NT5C3, or NT5C3A, gene on chromosome 7. ... nucleotidase family and one of the five cytosolic members identified in humans. NTC53 catalyzes the dephosphorylation of the ...
... nucleotidase (eN) as well as by APs. In any case, the final product of the hydrolysis cascade is the nucleoside. The Pannexin-1 ... nucleotidase (CD73) (Le Hir and Kaissling, 1993). This enzyme catalyzes the dephosphorylation of 5′-AMP or 5′-IMP to adenosine ... nucleotidase (Thomson et al., 2000). Extracellular adenosine activates A1 receptors on vascular afferent arteriolar smooth ... "Cellular function and molecular structure of ecto-nucleotidases". Purinergic Signalling. 8 (3): 437-502. doi:10.1007/s11302-012 ...
27 (5): 1489-1495. doi:10.1016/j.tiv.2013.03.002. PMID 23524101. Chou, Janice Y.; Robinson, J. C. (1977-01-01). "Induction of ... 56 (5): 326-329. Ninfa; Ballou; Benore (2010). Biochemistry and Biotechnology. USA: John Wiley & Sons, INC. pp. 229-230. ISBN ... 10 (5): e0124835. doi:10.1371/journal.pone.0124835. PMC 4422672 . PMID 25946026. CS1 maint: Uses authors parameter (link) Kay, ... doi:10.1186/s40697-015-0066-5. CS1 maint: Uses authors parameter (link) Mizumori, M., Ham, M., Guth, P. H., Engel, E., Kaunitz ...
... nucleotidase activity in workers exposed to lead, mercury or cadmium". Int Arch Occup Environ Health. 55 (3): 247-52. doi: ... nucleotidase, important for the correct metabolism of DNA and can therefore cause fetal damage. Chemistry portal Pigments ... 5 (3): 242-242. doi:10.1021/je60007a002. Pauley, J. L.; M. K. Testerman (1954). "Basic Salts of Lead Nitrate Formed in Aqueous ... 5 (3): 242. doi:10.1021/je60007a002. "Historical development of titanium dioxide". Millennium Inorganic Chemicals. Archived ...
A nucleotidase is an enzyme that catalyzes the hydrolysis of a nucleotide, forming a nucleoside and a phosphate ion. ... Inside the cell, nucleotidases may help to maintain energy levels under stress conditions. A cell deprived of oxygen and ... Some nucleotidases function outside the cell, creating nucleosides that can be transported into the cell and used to regenerate ... Nucleotidases are essential for cellular homeostasis, because they are partially responsible for maintaining a balanced ratio ...
... nucleotidase (5NCT) family, is also capable of dephosphorylating cladribine, making it inactive. The most important subtype of ... 5 (1): 1-8. doi:10.3109/10428199109068099. PMID 27463204.. *^ Baker, D; Marta, M; Pryce, G; Giovannoni, G; Schmierer, K ( ... 362 (5): 416-26. doi:10.1056/NEJMoa0902533. PMID 20089960.. *^ Johnston, JB (June 2011). "Mechanism of action of pentostatin ... 52 Suppl 2: 43-5. doi:10.3109/10428194.2011.570394. PMID 21463108.. *^ Beutler, E; Piro, LD; Saven, A; Kay, AC; McMillan, R; ...
... nucleotidase activity via Ca2+-dependent, annexin 2-mediated membrane rearrangement?". Biochemical Society Transactions. 34 (Pt ... 33 (5): 1223-8. doi:10.1021/bi00171a023. PMID 8110754. Wright JF, Kurosky A, Wasi S (Feb 1994). "An endothelial cell-surface ... Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". The Journal of Biological Chemistry. 277 ( ... doi:10.1016/S0300-9084(02)00027-5. PMID 12628297. Kirshner J, Schumann D, Shively JE (Dec 2003). "CEACAM1, a cell-cell adhesion ...
... nucleotidase) in 165 glioblastomas by immunohistochemistry and electronmicroscopic histochemistry". Anticancer Res. 19 (3A): ... 272 (5): 2814-20. doi:10.1074/jbc.272.5.2814. ISSN 0021-9258. PMID 9006922. Talts, J F; Sasaki T; Miosge N; Göhring W; Mann K; ... 5 (4): 932-5. doi:10.1016/0888-7543(89)90136-5. PMID 2591971. "Entrez Gene: LAMA1 laminin, alpha 1". Utani, A; Nomizu M; Yamada ... Utani A, Nomizu M, Yamada Y (1997). "Fibulin-2 binds to the short arms of laminin-5 and laminin-1 via conserved amino acid ...
... nucleotidase and ALP enzymes. With a few exceptions, the optimal test for cholestasis would be elevations of serum bile acid ... Cholestasis can be suspected when there is an elevation of both 5'- ...
... nucleotidase deficiency Thrombotic thrombocytopenic purpura Stippling Basophile Cheson, B.D.; Rom, W. N.; Webber, R. C. (1984 ... nucleotidase deficiency, and intraerythrocytic accumulation of pyrimidines". J. Clin. Invest. 58 (4): 926-32. doi:10.1172/ ... 5 (4): 327-34. PMID 6202140. "Pathology". Retrieved 2009-02-08. Valentine WN, Paglia DE, Fink K, Madokoro G (October 1976). " ... "Lead poisoning: association with hemolytic anemia, basophilic stippling, erythrocyte pyrimidine 5'- ...
... nucleotidase and decrease in guanylate kinase activity in the brain and liver of irradiated rats]". Ukr. Biokhim. Zh. 62 (2): ... Boritzki TJ, Jackson RC, Morris HP, Weber G (1981). "Guanosine-5'-phosphate synthetase and guanosine-5'-phosphate kinase in rat ...
... nucleotidase I activity in MOLT-4 cells". Nucleosides Nucleotides Nucleic Acids. 25 (9-11): 1201-4. doi:10.1080/ ... 62 (5): 745-7. doi:10.1001/archneur.62.5.745. PMID 15883261. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N ... 293 (5): 1489-96. doi:10.1016/S0006-291X(02)00415-1. PMID 12054684. Salviati L, Sacconi S, Mancuso M, Otaegui D, Camaño P, ... 11 (5): 677-84. doi:10.1101/gr.164001. PMC 311086 . PMID 11337467. Johansson K, Ramaswamy S, Ljungcrantz C, Knecht W, Piskur J ...
... nucleotidases (EC), 3'-nucleotidases (EC) and exopolyphosphatases (EC). The gene, surE, is part of a bicistronic operon found ... 326 (5): 1559-75. doi:10.1016/S0022-2836(03)00056-1. PMID 12595266. This article incorporates text from the public domain Pfam ... This domain is found in acid phosphatases (EC), 5'- ...
... nucleotidase". Biochim. Biophys. Acta. 33 (1): 101-5. doi:10.1016/0006-3002(59)90502-5. PMID 13651188. Molecular and Cellular ... In enzymology, a 5'-acylphosphoadenosine hydrolase (EC 3.6.1.20) is an enzyme that catalyzes the chemical reaction 5'- ... The systematic name of this enzyme class is 5'-acylphosphoadenosine acylhydrolase. This enzyme is also called 5- ... KELLERMAN GM (1959). "Isolation and characteristics of the enzyme acyl 5'- ...
... -Activating Nucleotidase).[26] The association of the 19S and 20S particles requires the binding of ATP to the 19S ... 66 (5): 309-317. doi:10.1002/iub.1271. PMID 24823973.. *^ a b Nobel Prize Committee (2004). "Nobel Prize Awardees in Chemistry ... 109 (5): 671-9. doi:10.1172/JCI12736. PMC 150886. PMID 11877475.. *^ Elliott PJ, Pien CS, McCormack TA, Chapman ID, Adams J ( ... 5 (22): 2592-601. doi:10.4161/cc.5.22.3448. PMID 17106261.. *^ Park SH, Bolender N, Eisele F, Kostova Z, Takeuchi J, Coffino P ...
... nucleotidase (5-NT) is a protein produced by the liver. A test can be done to measure the amount of this protein in your blood ... nucleotidase (5-NT) is a protein produced by the liver. A test can be done to measure the amount of this protein in your blood ...
... nucleotidase, thimidine monophosphate nucleotidase, 5-AMPase, 5-AMP nucleotidase, AMP phosphohydrolase, IMP 5-nucleotidase ... Reis, Julian (1934). "Nucleotidase and its relation to the deamination of nucleotides in the heart and the muscles". Bulletin ... Nucleotidase Levels Blood Test. Ecto-5Nucleotidase is considered a maturation marker for T cells and B cells. This is due to ... nucleotidase and has an extracellular location. The two cytosolic forms of the enzyme have similar characteristics, but can be ...
Nucleotidase-positive and-negative subpopulations, J.Clin.Invest. 56: 1324-1327 (1975).PubMedCrossRefGoogle Scholar ... Nucleotidase as a Leukemia Marker. In: De Bruyn C.H.M.M., Simmonds H.A., Müller M.M. (eds) Purine Metabolism in Man-IV. ... Nucleotidase (5′-N) as an ectoenzyme has the double advantage of being testable by an ordinary enzyme assay on intact cells and ... We have characterized leukemic cells using 5′-N not as an isolated marker but rather within a battery of other markers ...
... nucleotidase. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and definitions. ... nucleotidase. Definition: an enzyme that catalyzes the hydrolysis of a pyrimidine-nucleoside 5-monophosphate to produce ...
Other names in common use include phosphoadenylate 3-nucleotidase, 3-phosphoadenylylsulfate 3-phosphatase, phosphoadenylate ... bisphosphate nucleotidase (EC 3.1.3.7) is an enzyme that catalyzes the chemical reaction adenosine 3,5-bisphosphate + H2O ... Bisphosphate nucleotidase". J. Biol. Chem. 262 (21): 10044-7. PMID 3038862. Lik-Shing Tsang M, Schiff JA (1976). "Properties of ... 3-nucleotidase, and 3(2),5-bisphosphonucleoside 3(2)-phosphohydrolase. This enzyme participates in sulfur metabolism. As ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
... nucleotidase of guinea pig polymorphonuclear leukocytes is localized exclusively on the plasma membrane of intact cells. The ...
Nucleotidase, C-terminal domain superfamily (IPR036907). Showing 1 to 20 of 355 results ...
... nucleotidase, ectoprovided by RGD. Primary source. RGD:61956 See related. Ensembl:ENSRNOG00000011071 Gene type. protein coding ... nucleotidase family [Nucleotide transport and metabolism, Defense mechanisms]. cd07409. Location:31 → 312. MPP_CD73_N; CD73 ... nucleotidase. Names. 5-NT. ecto-5-nucleotidase. NP_067587.2. *EC 3.1.3.5 ... nucleotidase and related proteins, N-terminal metallophosphatase domain. pfam02872. Location:341 → 509. 5_nucleotid_C; 5- ...
... nucleotidase (5N) (E.C.-3.1.3.5) is present in lymphocytes isolated from the blood of normal subjects. This activity is ... Citation Information: J Clin Invest. 1975;56(5):1324-1327. https://doi.org/10.1172/JCI108209. View: Text , PDF ...
Nucleotidase with a broad substrate specificity as it can dephosphorylate various ribo- and deoxyribonucleoside 5- ...
... nucleotidase phosphodiesterase hereditary degeneration of the retina Laboratory of Biochemical Bases of Reception, I. M. ... nucleotidase activity in heriditary degeneration of the retina. ... 5.. A. L. Berman, A. M. Azimova, and F. G. Gribakin, Vision Res ...
Nucleotidase/CD73 products for your research including 5-Nucleotidase/CD73 Primary Antibodies, Proteins and Enzymes, Small ... Nucleotidase/CD73: Products. CD73, an ecto-5-prime-nucleotidase, is a glycosyl phophatidylinositol-anchored ectoenzyme that ... The 5-prime-nucleotidase activity of CD73 converts extracellular nucleoside 5-prime monophosphates to nucleosides. It is one of ... Adenosine 5-(α,β-methylene)diphosphate sodium salt Ecto-5-nucleotidase (CD73) inhibitor ...
... nucleotidase. Mouse monoclonal antibody clone AD2 is used for FC. ... nucleotidase expressed on the surface of subsets of B and T ... CD73 is a glycosyl phosphatidylinositol-anchored glycoprotein and ecto-5- ... Nucleotidase,human antibody Ecto-5-Nucleotidase AD2,human antibody Ect ... Figure 5. Data for PE-Conjugated. (A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on ...
Nucleotidase with a broad substrate specificity as it can dephosphorylate various ribo- and deoxyribonucleoside 5- ... Belongs to the SurE nucleotidase family.UniRule annotation. ,p>Manual validated information which has been generated by the ... Nucleotidase with a broad substrate specificity as it can dephosphorylate various ribo- and deoxyribonucleoside 5- ... nucleotidase SurEUniRule annotation. ,p>Manual validated information which has been generated by the UniProtKB automatic ...
... nucleotidase/CD73 (e-5′NT/CD73), the enzyme responsible for adenosine formation from AMP. e-5′NT/CD73-/- mice were viable and ... e-5. ′. NT/CD73. mRNA transcripts in e-5. ′. NT/CD73+/+. (WT) and e-5. ′. NT/CD73-/-. mice (KO). Primer sequences are located ... e-5′NT/CD73-/- mice had a significantly reduced fall in stop flow pressure and superficial nephron glomerular filtration rate ... Blood pressure, blood and urine chemistry, and renal blood flow were not different between e-5′NT/CD73+/+ and e-5′NT/CD73-/- ...
... nucleotidase (CD73) regulation by hypoxia-inducible factor-1 mediates permeability changes in intestinal epithelia.. ... nucleotidase (CD73) regulation by hypoxia-inducible factor-1 mediates permeability changes in intestinal epithelia ... nucleotidase (CD73) regulation by hypoxia-inducible factor-1 mediates permeability changes in intestinal epithelia ... nucleotidase (CD73) regulation by hypoxia-inducible factor-1 mediates permeability changes in intestinal epithelia ...
Nucleotidase/CD73 Antibody (AD2) cited in 15 publications. Validated: Flow, Flow-CS, IHC-Fr. Tested Reactivity: Human. ... Nucleotidase/CD73.. Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target. By Yoskaly Lazo-Fernandez, ... Nucleotidase/CD73 Antibody (NBP2-48480). (Showing 1 - 1 of 1 FAQs).. * We are interested in CD73 antibodies that could react ... nucleotidase (CD73) in normal mammary gland and in breast carcinoma. Br J Cancer. 1991 Jan 1 [PMID: 1989648] (IHC-Fr, Human). ...
Nucleotidase/CD73 Antibody (45M3B4) [HRP]. Validated: WB. Tested Reactivity: Human, Mouse, Rat, and more. 100% Guaranteed. ... Nucleotidase/CD73 Antibody (45M3B4) [HRP] Summary. Immunogen. Within the range of amino acids 169-324 of human CD73 were used ... Nucleotidase/CD73.. Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target. By Yoskaly Lazo-Fernandez, ... Nucleotidase/CD73 Antibody (NBP2-25235H). (Showing 1 - 1 of 1 FAQ).. * We are interested in CD73 antibodies that could react ...
One factor may be the level of AMP-hydrolyzing activity attributed to enzymes other than CD73 (i.e., other nucleotidases and/or ... Nucleotidase (CD73) in Vascular Leakage during Hypoxia. Linda F. Thompson, Holger K. Eltzschig, Juan C. Ibla, C. Justin Van De ... nucleotidase activity and cell surface expression in endothelial cells: role of membrane lipids. Circ. Res. 92:848-855. ... Nucleotidase from smooth muscle of small intestine and from brain. Inhibition by nucleotides. Biochemistry. 14:2362-2366. ...
... nucleotidase from Torpedo electric organ E J M Grondal E J M Grondal ... Nucleotidase was isolated from the electric organ of the electric ray Torpedo marmorata after solubilization in Triton X-100 ... nucleotidase from electric-ray ( Torpedo marmorata ) electric organ and bovine cerebral cortex is derived from the glycosyl- ... nucleotidase from Torpedo marmorata (electric ray) electric organ Biochem J (August, 1991) ...
... nucleotidase) Antibody - CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ... nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5-NT Isotype Mouse IgG1, κ Ave. Rating Submit a Review Product Citations publications ... nucleotidase, 70 kD Distribution Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, ... nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, ...
... bisphosphate nucleotidase 1 (HMDBP00342). IdentificationBiological propertiesGene propertiesProtein propertiesExternal links ... nucleotidase inhibited by inositol 1,4-bisphosphate. J Biol Chem. 1999 May 7;274(19):13619-28. [PubMed:10224133 ] ... Adenosine 3,5-diphosphate + Water → Adenosine monophosphate + Phosphate. details Phosphoadenosine phosphosulfate + Water → ... Converts adenosine 3-phosphate 5-phosphosulfate (PAPS) to adenosine 5-phosphosulfate (APS) and 3(2)-phosphoadenosine 5- ...
... nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain family. Additional information, provided for both this family and ... nucleotidase activity. 0. 2.376. --. DIRECT. Molecular Function (MF). phosphoric ester hydrolase activity. 0. 1.406. --. DIRECT ... 3-nucleotidase. 0.0000001668. 2.264. --. DIRECT. Enzyme Commission (EC). Acting on ester bonds. 0.0000000001597. 0.5107. Least ... nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain family. SCOP classification Root: SCOP hierarchy in SUPERFAMILY [. 0 ...
Keywords:TECHNOLOGY; Bioengineering; Structural biochemistry; Nucleotidases; nucleotide; nucleoside; nucleoside analogue; ... nucleotidase II were used to characterize the structural basis for their substrate specificity in high detail.Based on ... nucleotidase II, a mechanism for the allosteric activation of this enzyme was presented. In this mechanism, the activator ... Nucleotidases (5NTs) are catabolic enzymes of the nucleotide metabolism. They catalyze dephosphorylation of deoxyribo- and ...
  • 5'-nucleotidase (5'-NT) is a protein produced by the liver. (medlineplus.gov)
  • The high affinity of ND to PAP and commercial availability for PAP agarose (the cross-linking product of activated agarose and the free amino group from N6-[(6-amino)octyl]-amino-adenosine-3',5'-bisphosphate, Sigma-aldrich,#A3640) enabled affinity purification of YND which suggested YND could be used as a possible fusion affinity tag for protein purification.Affinity chromatography is one of the most popular methods for protein purification. (globethesis.com)
  • Therefore, the present study reports that the activity and protein abundance of membrane-bound eN fluctuates across the estrus cycle in the hippocampal synaptosomes of female rats. (ac.rs)
  • Selective ERa receptor agonist, PPT, induced upregulation of both the protein level and activity of eN, while application of selective ERb receptor agonist, DPN, increased only the activity of eN. (ac.rs)
  • Of most interest, SNPs (linkage disequilibrium group CEU.12) in the 5′-untranslated region were associated with NT5C2 expression and ara-C sensitivity in HapMap cell lines and with NT5C2 mRNA expression and ara-C sensitivity in diagnostic leukemic blasts from pediatric patients with acute myeloid leukemia. (aspetjournals.org)
  • Orthologues of Streptococcus pyogenes nuclease A (SpnA) and Streptococcal 5′-nucleotidase A... Soh, Kar Yan;Loh, Jacelyn Mei San;Proft, Thomas 2018-04-05 00:00:00 Abstract Streptococcus pyogenes nuclease A (SpnA) and streptococcal 5′ nucleosidase A (S5nA) are two recently described virulence factors from the human pathogen S. pyogenes. (deepdyve.com)
  • Spiegelberg BD, Xiong JP, Smith JJ, Gu RF, York JD: Cloning and characterization of a mammalian lithium-sensitive bisphosphate 3'-nucleotidase inhibited by inositol 1,4-bisphosphate. (hmdb.ca)
  • Polyclonal antibodies raised against 5′‐nucleotidase from mammalian sources or the electric ray electric organ reveal mutual crossreactivity. (elsevier.com)
  • Further, we analyzed the role of E2 and its intracellular receptors on the expression of eN in ovariectomized females. (ac.rs)
  • The bovine cDNA sequence included the sequences codifying for six peptides obtained from 5ʹ-nucleotidase/phosphotransferase purified from calf thymus. (biochemj.org)
  • A cDNA encoding a 5′‐nucleotidase was identified by screening a λgt10 cDNA library from the electric lobe of Discopyge ommata using a cDNA probe containing the complete open reading frame coding for the rat liver enzyme. (elsevier.com)
  • Seven residues from conserved sequence motifs implicated in the catalytic and metal-ion-binding sites of E. coli 5′-nucleotidase (Asp 41 , His 43 , Asp 84 , His 117 , Glu 118 , His 217 and His 252 ) were selected for modification using site-directed mutagenesis of the cloned ushA gene. (biochemj.org)
  • Non-enzymatic functions of eN have also been reported. (edu.pl)
  • Phylogenetic analysis of the 5'-nucleotidase family revealed that the O. savignyi 5'-nucleotidase isoforms claded monophyletically with the putative apyrases from I. scapularis and R. appendiculatus, excluding the 5'-nucleotidase from R. (B.) microplus. (up.ac.za)