A class of enzymes that catalyze the conversion of a nucleotide and water to a nucleoside and orthophosphate. EC 3.1.3.-.
A glycoprotein enzyme present in various organs and in many cells. The enzyme catalyzes the hydrolysis of a 5'-ribonucleotide to a ribonucleoside and orthophosphate in the presence of water. It is cation-dependent and exists in a membrane-bound and soluble form. EC 3.1.3.5.
A calcium-activated enzyme that catalyzes the hydrolysis of ATP to yield AMP and orthophosphate. It can also act on ADP and other nucleoside triphosphates and diphosphates. EC 3.6.1.5.
Cytidine (dihydrogen phosphate). A cytosine nucleotide containing one phosphate group esterified to the sugar moiety in the 2', 3' or 5' position.
A species of halophilic archaea found in the Dead Sea.
Pyrimidines with a RIBOSE and phosphate attached that can polymerize to form DNA and RNA.
A disease of cats and mink characterized by a marked inflammation of adipose tissue and the deposition of "ceroid" pigment in the interstices of the adipose cells. It is believed to be caused by feeding diets containing too much unsaturated fatty acid and too little vitamin E. (Merck Veterinary Manual, 5th ed; Stedman, 25th ed)
A condition of inadequate circulating red blood cells (ANEMIA) or insufficient HEMOGLOBIN due to premature destruction of red blood cells (ERYTHROCYTES).
5'-Uridylic acid. A uracil nucleotide containing one phosphate group esterified to the sugar moiety in the 2', 3' or 5' position.
Adenine nucleotide containing one phosphate group esterified to the sugar moiety in the 2'-, 3'-, or 5'-position.
A group of hydrolases which catalyze the hydrolysis of monophosphoric esters with the production of one mole of orthophosphate. EC 3.1.3.
A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.
A group of enzymes within the class EC 3.6.1.- that catalyze the hydrolysis of diphosphate bonds, chiefly in nucleoside di- and triphosphates. They may liberate either a mono- or diphosphate. EC 3.6.1.-.
A nucleoside that is composed of ADENINE and D-RIBOSE. Adenosine or adenosine derivatives play many important biological roles in addition to being components of DNA and RNA. Adenosine itself is a neurotransmitter.
The rate dynamics in chemical or physical systems.
Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.
Proteins found in any species of archaeon.
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
The monomeric units from which DNA or RNA polymers are constructed. They consist of a purine or pyrimidine base, a pentose sugar, and a phosphate group. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
A large multisubunit complex that plays an important role in the degradation of most of the cytosolic and nuclear proteins in eukaryotic cells. It contains a 700-kDa catalytic sub-complex and two 700-kDa regulatory sub-complexes. The complex digests ubiquitinated proteins and protein activated via ornithine decarboxylase antizyme.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The sum of the weight of all the atoms in a molecule.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
Animals having a vertebral column, members of the phylum Chordata, subphylum Craniata comprising mammals, birds, reptiles, amphibians, and fishes.
A phosphoric diester hydrolase that removes 5'-nucleotides from the 3'-hydroxy termini of 3'-hydroxy-terminated OLIGONUCLEOTIDES. It has low activity towards POLYNUCLEOTIDES and the presence of 3'-phosphate terminus on the substrate may inhibit hydrolysis.
Solutions or mixtures of toxic and nontoxic substances elaborated by snake (Ophidia) salivary glands for the purpose of killing prey or disabling predators and delivered by grooved or hollow fangs. They usually contain enzymes, toxins, and other factors.
An array of tests used to determine the toxicity of a substance to living systems. These include tests on clinical drugs, foods, and environmental pollutants.
The highest dosage administered that does not produce toxic effects.
Experiments designed to determine the potential toxic effects of a long-term exposure to a chemical or chemicals.
Experiments designed to determine the potential toxic effects of one-time, short-term exposure to a chemical or chemicals.
Laws and regulations concerned with industrial processing and marketing of foods.
Components of the usual diet that may provide health benefits beyond basic nutrients. Examples of functional foods include soy, nuts, chocolate, and cranberries (From NCCAM Backgrounder, March 2004, p3).
Experiments designed to determine the potential toxic effects of mid-term (a few months) exposure to a chemical or chemicals.
Nucleotidase (5NT) is a glycoprotein found throughout the body, in the cytoplasmic membrane, catalyzing the conversion to ... 45 (5): 558-568. PMC 4935699. PMID 27398328.. *^ a b Kasper, Dennis L.; Fauci, Anthony S.; Hauser, Stephen L.; Longo, Dan L.; ... Retrieved 5 August 2011.. *^ Johnston DE (1999). "Special considerations in interpreting liver function tests". Am Fam ... Pathological jaundice in newborns should be suspected when the serum bilirubin level rises by more than 5 mg/dL per day, serum ...
... nucleotidase (cN1A; NT5C1A) have been strongly associated with the condition. However, other inflammatory conditions, such as ... 15 (5): 257-272. doi:10.1038/s41584-019-0186-x. PMID 30837708. S2CID 71146208. Machado, P; Dimachkie, MM; Bahron, RJ (October ... The earliest published prevalence was in 2000 and put at 5 per million. A 2017 study in Ireland reported 112 per million. It is ... 27 (5): 591-598. doi:10.1097/WCO.0000000000000129. PMC 4248565. PMID 25159931. Machado, P; Brady, S; Hanna, MG (2013). "Update ...
... nucleotidase, cytosolic IA is a protein that in humans is encoded by the NT5C1A gene. Cytosolic nucleotidases, such as NT5C1A, ... nucleotidase I: characterization and role in nucleoside analog resistance". J. Biol. Chem. 276 (13): 10498-504. doi:10.1074/jbc ... nucleotidase, cytosolic IA". Hunsucker SA, Spychala J, Mitchell BS (2001). "Human cytosolic 5'- ...
... nucleotidase 2 (dNT-2), is an enzyme that in humans is encoded by the NT5M gene. This gene encodes a 5' nucleotidase that ... nucleotidase, mitochondrial". Rampazzo C, Gallinaro L, Milanesi E, Frigimelica E, Reichard P, Bianchi V (Jul 2000). "A ... nucleotidases identified in humans, all of which differ in tissue specificity, subcellular location, primary structure and ... nucleotidase, mitochondrial, also known as 5'(3')-deoxyribonucleotidase, mitochondrial (mdN) or deoxy-5'- ...
... nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. CD73 commonly ... nucleotidase, ecto (CD73)". St Hilaire C, Ziegler SG, Markello TC, Brusco A, Groden C, Gill F, et al. (February 2011). "NT5E ... nucleotidase) at the PDBe-KB. This article incorporates text from the United States National Library of Medicine, which is in ... nucleotidase of intact cultured C6 rat glioma cells". The Journal of Biological Chemistry. 251 (13): 3900-5. doi:10.1016/S0021- ...
Of the different forms of nucleotidases characterized, this enzyme is unique in its preference for 5'-dNTP. It may be one of ... This gene encodes a nucleotidase that catalyzes the dephosphorylation of the 5' deoxyribonucleotides (dNTP) and 2'(3')-dNTP and ... nucleotidase mRNA levels in blast cells are a prognostic factor in acute myeloid leukemia patients treated with cytarabine". ... nucleotidase mRNA levels in blast cells are a prognostic factor in acute myeloid leukemia patients treated with cytarabine". ...
... nucleotidase as well as of a number of the nucleoside triphosphate and diphosphate-hydrolyzing enzymes of the family of the ... nucleotidase from the electric ray electric lobe. Primary structure and relation to mammalian and procaryotic enzymes". Biochem ... nucleotidase from Torpedo electric organ". Biochemical Journal. 245 (3): 805-810. doi:10.1042/bj2450805. PMC 1148201. PMID ... "Cellular function and molecular structure of ecto-nucleotidases". Purinergic Signal. 8 (3): 437-502. doi:10.1007/s11302-012- ...
... nucleotidase, and phosphodiesterase. The skin of the blue-spotted stingray is often used for drums, such as on the Arab and ...
1995). "Homogeneous pyrimidine nucleotidase from human erythrocytes: enzymic and molecular properties". Biochem. J. 304 (Pt 3 ... 1998). "Pyrimidine nucleotidases from human erythrocyte possess phosphotransferase activities specific for pyrimidine ... nucleotidase (PN-I or P5'NI), and p56, is an enzyme that in humans is encoded by the NT5C3, or NT5C3A, gene on chromosome 7. ... nucleotidase family and one of the five cytosolic members identified in humans. NTC53 catalyzes the dephosphorylation of the ...
... nucleotidase and ALP enzymes. With a few exceptions, the optimal test for cholestasis would be elevations of serum bile acid ... nucleotidase as a liver-function test". The Quarterly Journal of Medicine. 36 (144): 457-468. doi:10.1093/oxfordjournals.qjmed. ... 447 (5): 566-570. doi:10.1007/s00424-003-1130-z. PMID 12851823. S2CID 35115446. Xiao J, Li Z, Song Y, Sun Y, Shi H, Chen D, ... 47: 1-5. doi:10.1016/j.ejim.2017.06.020. PMID 28669591. Corpechot C, Abenavoli L, Rabahi N, Chrétien Y, Andréani T, Johanet C, ...
... nucleotidase. The nucleoside, adenosine, is then deaminated and hydrolyzed to form hypoxanthine via adenosine deaminase and ... Nelson, David L.; Cox, Michael M.; Lehninger, Albert L. (2008). Lehninger's Principles of Biochemistry (5 ed.). Macmillan. ISBN ... First, the nucleotide will lose its phosphate through 5'- ...
... nucleotidase (CD73) and A2B adenosine receptors". Circulation. 115 (12): 1581-90. doi:10.1161/CIRCULATIONAHA.106.669697. PMID ... 5 (6): e137. doi:10.1371/journal.pmed.0050137. PMC 2504049. PMID 18578565. Eckle T, Grenz A, Laucher S, Eltzschig HK (October ...
... nucleotidase family". Exp. Parasitol. 122 (4): 318-27. doi:10.1016/j.exppara.2009.04.007. PMID 19393241. B.J. Mans; A.W.H. ... 13 (1): 153-5. doi:10.3201/eid1301.061094. PMC 2725816. PMID 17370534. Rico-Hesse, Rebeca; Dodd, Kimberly A.; Bird, Brian H.; ... 15 (5-6): 570-571. doi:10.1093/jmedent/15.5-6.570. Schlein, Y.; Gunders, A. E. (2009). "Pheromone of Ornithodoros spp. ( ... 5 (10): e1352. doi:10.1371/journal.pntd.0001352. PMC 3186760. PMID 21991403. "Archived copy". Archived from the original on ...
... nucleotidase. Adenosine constricts the afferent arteriole by binding with high affinity to the A1 receptors a Gi/Go. Adenosine ... nucleotidase (CD73) (Le Hir and Kaissling, 1993). This enzyme catalyzes the dephosphorylation of 5′-AMP or 5′-IMP to adenosine ... nucleotidase (Thomson et al., 2000). Extracellular adenosine activates A1 receptors on vascular afferent arteriolar smooth ... 299 (2): F310-5. doi:10.1152/ajprenal.00149.2010. PMC 2928524. PMID 20462966. Ortiz-Capisano MC, Atchison DK, Harding P, Lasley ...
27 (5): 1489-95. doi:10.1016/j.tiv.2013.03.002. PMID 23524101. Chou JY, Robinson JC (July 1977). "Induction of placental ... 5 (4): 817-30. doi:10.1016/s0950-3552(05)80289-5. PMID 1822819. Retrieved 1 May 2016. Lange PH, Millan JL, Stigbrand T, ... 56 (5): 326-329. Archived from the original (PDF) on 2011-07-25. Retrieved 2009-04-28. "Alkaline Phosphatase Level Test (ALP ... doi:10.1186/s40697-015-0066-5. PMC 4538753. PMID 26284153. Mizumori M, Ham M, Guth PH, Engel E, Kaunitz JD, Akiba Y (July 2009 ...
... nucleotidase deficiency--congenital hemolytic anemia with basophilic stippling of erythrocytes". Tidsskrift for den Norske ... nucleotidase deficiency Alcoholism Myelodysplastic syndromes Sideroblastic anemia Congenital dyserythropoietic anemia Primary ... 5 (4): 327-34. doi:10.1002/ajim.4700050409. PMID 6202140. Tkachuk, Douglas C.; Hirschmann, Jan V.; Wintrobe, Maxwell Myer (2007 ... Severe megaloblastic anemia Hemolytic anemia Sickle-cell anemia Pyrimidine 5' ...
... nucleotidase, important for the correct metabolism of DNA and can therefore cause fetal damage. CRC handbook of chemistry and ... nucleotidase activity in workers exposed to lead, mercury or cadmium". Int Arch Occup Environ Health. 55 (3): 247-52. doi: ... At higher pH Pb6(OH)5(NO3) is formed. The cation [Pb6O(OH)6]4+ is unusual in having an oxide ion inside a cluster of 3 face- ... 5 (3): 242. doi:10.1021/je60007a002. Pauley, J. L.; M. K. Testerman (1954). "Basic Salts of Lead Nitrate Formed in Aqueous ...
A nucleotidase is an enzyme that catalyzes the hydrolysis of a nucleotide, forming a nucleoside and a phosphate ion. ... Inside the cell, nucleotidases may help to maintain energy levels under stress conditions. A cell deprived of oxygen and ... Some nucleotidases function outside the cell, creating nucleosides that can be transported into the cell and used to regenerate ... Nucleotidases are essential for cellular homeostasis, because they are partially responsible for maintaining a balanced ratio ...
... nucleotidase activity via Ca2+-dependent, annexin 2-mediated membrane rearrangement?". Biochemical Society Transactions. 34 (Pt ... 33 (5): 1223-1228. doi:10.1021/bi00171a023. PMID 8110754. Wright JF, Kurosky A, Wasi S (February 1994). "An endothelial cell- ... Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". The Journal of Biological Chemistry. 277 ( ... doi:10.1016/S0300-9084(02)00027-5. PMID 12628297. Kirshner J, Schumann D, Shively JE (December 2003). "CEACAM1, a cell-cell ...
... nucleotidase activities in physiological and disease conditions: New perspectives for human health". BioFactors. 31 (2): 77-98 ... 140 (5): 619-630. doi:10.1016/j.cell.2010.02.014. ISSN 0092-8674. PMID 20211132. Peter, C.; Wesselborg, S.; Lauber, K. (2009-12 ... 10 (5): 556-566. doi:10.1038/ncb1718. ISSN 1476-4679. PMC 2851549. PMID 18425118. Chekeni, Faraaz B.; Elliott, Michael R.; ... 79 (5): 896-903. doi:10.1189/jlb.1005550. Ravichandran, Kodi S.; Lorenz, Ulrike (December 2007). "Engulfment of apoptotic cells ...
... nucleotidase) in 165 glioblastomas by immunohistochemistry and electronmicroscopic histochemistry". Anticancer Res. 19 (3A): ... 272 (5): 2814-20. doi:10.1074/jbc.272.5.2814. ISSN 0021-9258. PMID 9006922. S2CID 24800195. Talts, J F; Sasaki T; Miosge N; ... 5 (4): 932-5. doi:10.1016/0888-7543(89)90136-5. PMID 2591971. "Entrez Gene: LAMA1 laminin, alpha 1". Utani, A; Nomizu M; Yamada ... Utani A, Nomizu M, Yamada Y (1997). "Fibulin-2 binds to the short arms of laminin-5 and laminin-1 via conserved amino acid ...
Zimmermann H, Zebisch M, Sträter N (September 2012). "Cellular function and molecular structure of ecto-nucleotidases". ... nucleotidase (eN) as well as by APs. In any case, the final product of the hydrolysis cascade is the nucleoside. The Pannexin-1 ... nucleotidase (CD73) (Le Hir and Kaissling, 1993). This enzyme catalyzes the dephosphorylation of 5′-AMP or 5′-IMP to adenosine ... nucleotidase (Thomson et al., 2000). Extracellular adenosine activates A1 receptors on vascular afferent arteriolar smooth ...
... nucleotidase which has been extensively characterized kinetically. The tetrameric enzyme shows aspects of positive homotropic ... "Allosteric regulation and substrate activation in cytosolic nucleotidase II from Legionella pneumophila". FEBS Journal. 281 (6 ... 193 (5): 1114-21. doi:10.1128/JB.01146-10. PMC 3067580. PMID 21169481. Cianciotto, Nicholas P (May 2015). "An update on iron ... 9 (5): 1313. doi:10.1128/mBio.01313-18. PMC 6178616. PMID 30301851. Michod RE, Bernstein H, Nedelcu AM (May 2008). "Adaptive ...
Srinivasan B, Balaram H (2007). "ISN1 nucleotidases and HAD superfamily protein fold: in silico sequence and structure analysis ... nucleotidases catalyze the hydrolysis of purine mononucleotides to their respective nucleosides and phosphate. The nucleosides ... "Allosteric regulation and substrate activation in cytosolic nucleotidase II from Legionella pneumophila". The FEBS Journal. 281 ... Uridine kinase (aka uridine-cytidine kinase) can then phosphorylate the 5'-carbon of this nucleoside into uridine monophosphate ...
... nucleotidase deficiency, and intraerythrocytic accumulation of pyrimidines". Journal of Clinical Investigation. 58 (4): 926-932 ... Hemolytic anemia accounts for 5% of all existing anemias. It has numerous possible consequences, ranging from general symptoms ... 5), 1232-1237. https://doi.org/10.2215/CJN.01960408 Current Medical Diagnosis and Treatment 2009 By Stephen J. McPhee, Maxine A ...
... nucleotidase and decrease in guanylate kinase activity in the brain and liver of irradiated rats]". Ukr. Biokhim. Zh. 62 (2): ... Boritzki TJ, Jackson RC, Morris HP, Weber G (1981). "Guanosine-5'-phosphate synthetase and guanosine-5'-phosphate kinase in rat ... doi:10.1016/S0076-6879(78)51066-5. ISBN 978-0-12-181951-4. PMID 211390. Overview of all the structural information available in ...
... nucleotidase (5'-NT), which breaks down and inactivates the compound. This ratio differs between cell types, with high levels ... nucleotidase (5'-NT) family, is also capable of dephosphorylating cladribine, making it inactive. The most important subtypes ... Each treatment week consists of 4 or 5 days on which a patient receives 10 mg or 20 mg (1 or 2 tablets) as a single daily dose ... Accumulation of cladribine into cells is dependent on the ratio of DCK and 5'-NT. This ratio differs between cell types, with ...
... nucleotidase I activity in MOLT-4 cells". Nucleosides Nucleotides Nucleic Acids. 25 (9-11): 1201-4. doi:10.1080/ ... 62 (5): 745-7. doi:10.1001/archneur.62.5.745. PMID 15883261. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N ... 11 (5): 677-84. doi:10.1101/gr.gr-1640r. PMC 311086. PMID 11337467. Johansson K, Ramaswamy S, Ljungcrantz C, Knecht W, Piskur J ... 293 (5): 1489-96. doi:10.1016/S0006-291X(02)00415-1. PMID 12054684. Salviati L, Sacconi S, Mancuso M, Otaegui D, Camaño P, ...
... nucleotidases (EC), 3'-nucleotidases (EC) and exopolyphosphatases (EC). The gene, surE, is part of a bicistronic operon found ... 326 (5): 1559-75. doi:10.1016/S0022-2836(03)00056-1. PMID 12595266. This article incorporates text from the public domain Pfam ... This domain is found in acid phosphatases (EC), 5'- ...
... nucleotidase". Biochimica et Biophysica Acta. 33 (1): 101-5. doi:10.1016/0006-3002(59)90502-5. PMID 13651188. Portal: Biology v ... In enzymology, a 5'-acylphosphoadenosine hydrolase (EC 3.6.1.20) is an enzyme that catalyzes the chemical reaction 5'- ... This enzyme is also called 5-phosphoadenosine hydrolase. This enzyme participates in purine metabolism. Kellerman GM (May 1959 ... The systematic name of this enzyme class is 5'-acylphosphoadenosine acylhydrolase. ...
... nucleotidase), PITPNM2 (Membrane-associated phosphatidylinositol transfer protein 2), SBNO1 (strawberry notch homolog 1), ZEB2 ... 31 (5): 864-70. doi:10.1093/carcin/bgq051. PMC 2864416. PMID 20197299. Liu M, Lang N, Qiu M, Xu F, Li Q, Tang Q, Chen J, Chen X ... 68 (5): 1362-8. doi:10.1158/0008-5472.CAN-07-2912. PMID 18316599. Smrt RD, Szulwach KE, Pfeiffer RL, Li X, Guo W, Pathania M, ... 2 (5): 1233-1243. doi:10.1016/j.celrep.2012.09.033. ISSN 2211-1247. PMID 23168260. Landgraf P, Rusu M, Sheridan R, et al. (June ...
Blockade of P2-purinoceptor subtypes and ecto-nucleotidases by compounds related to Evans blue and trypan blue". Naunyn- ... Price, C. J.; Raymond, L. A. (December 1996). "Evans blue antagonizes both alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate ...
Dai Duy Ban and J.Kawiak: (Announcement in Poland in English): Nucleotidase of L 1210 Cells. Cytochemical and Kinetic Studies, ... Cell structure and Function 5, 211-215, 1980. Dai Duy Ban and Aleksandra Przetecka: (Announcement in Poland in English): ...
Angle, C. R.; McIntire, M. S. (1978). "Low level lead and inhibition of erythrocyte pyrimidine nucleotidase". Environmental ... 56 (5): 819-822. PMID 1196740. Angle, C. R.; McIntire, M. S.; Brunk, G. (1977). "Effect of anemia on blood and tissue lead in ... 5: 179-184. Angle, C. R.; Manton, W. I.; Stanek, K. L. (1995). "Stable isotope identification of lead sources in preschool ... 5 (5): 855-870. doi:10.1080/15287397909529795. PMID 583166. Angle, C. R.; Stohs, S. J.; McIntire, M. S.; Swanson, M. S.; Rovang ...
The nucleotidase and fosfatase incubation resulted in a reduction of the cytotoxicity of FdUMP, which means that the drug can ... The inhibition of the nucleotidase and fosfatase activity, decreased the effect of FdUMP, while the inhibitory effect was ... For 5-FU to inhibit TS by its mechanism of action, it must first be bioactivated through a series of reactions: 5-FU ends up ... Once the 5-FU or 5-FUdR prodrugs have been bioactivated resulting in FdUMP, they will already be recognized by the TS enzyme. ...
... nucleotidase, and phosphatase activities". The Journal of Biological Chemistry. 279 (35): 36819-36827. doi:10.1074/jbc. ... 11 (2): 100-5. doi:10.1016/j.mib.2008.02.001. PMID 18359660. Wendrich TM, Marahiel MA (October 1997). "Cloning and ... Spira B, Silberstein N, Yagil E (July 1995). "Guanosine 3',5'-bispyrophosphate (ppGpp) synthesis in cells of Escherichia coli ...
... then a nucleotidase creates inosine Purine nucleoside phosphorylase acts upon inosine to create hypoxanthine Xanthine oxidase ... catalyzes the oxidation of xanthine to uric acid A nuclease frees the nucleotide A nucleotidase creates adenosine, then ... A nuclease frees the nucleotide A nucleotidase creates guanosine Purine nucleoside phosphorylase converts guanosine to guanine ... 271 (14): 8192-5. doi:10.1074/jbc.271.14.8192. PMID 8626510. Garrett RH, Grisham CM (2016-02-11). Biochemistry (Sixth ed.). ...
... nucleotidases. This transcriptomic data together with the information provided by the high quality Indian cobra genome ... Besides, chromosomes 5 and 6 of the Indian cobra are syntenic to rattlesnake chromosome 4, indicating a possible fusion event ... For example, chromosome 4 of the Indian cobra shares syntenic regions with chromosomes 3 and 5 of the rattlesnake genome, ... doi:10.1016/0041-0101(95)00139-5. PMID 8735239. Smith, M.A. (1943). Reptilia and Amphibia, Volume 3: Serpentes. The Fauna of ...
... nucleotidase, thimidine monophosphate nucleotidase, 5′-AMPase, 5′-AMP nucleotidase, AMP phosphohydrolase, IMP 5′-nucleotidase ... Reis, Julian (1934). "Nucleotidase and its relation to the deamination of nucleotides in the heart and the muscles". Bulletin ... nucleotidase activity can be used as an index of lead poisoning. It is believed that lead induced deficiency of the enzyme in ... Nucleotidase Levels Blood Test. Ecto-5'Nucleotidase is considered a maturation marker for T cells and B cells. This is due to ...
March 2014). "Allosteric regulation and substrate activation in cytosolic nucleotidase II from Legionella pneumophila". The ... 1800 (1): 1-5. doi:10.1016/j.bbagen.2009.10.007. PMID 19840832. Beal SL (December 1983). "Computation of the explicit solution ... 1770 (5): 733-746. doi:10.1016/j.bbagen.2007.01.001. PMID 17307293. Srinivasan B, Skolnick J (May 2015). "Insights into the ... 5 (3): 261-269. doi:10.2174/1389201043376887. PMID 15180547. Schnell JR, Dyson HJ, Wright PE (2004). "Structure, dynamics, and ...
Nucleotidase Ecto (NT5E) gene. This gene is found on the long arm of chromosome 6 (6q14.3). The protein hydrolyies ... N Engl J Med 364(5):432-42 (Articles with short description, Short description matches Wikidata, All articles with unsourced ... This condition is caused by mutations in the 5'- ...
... nucleotidase, binding of one GMP molecule to a single subunit of the tetrameric enzyme leads to increased affinity for GMP by ... nucleotidase II (cN-II), where the affinity for substrate GMP increases upon GTP binding at the dimer interface. Negative ... nucleotidase, binding of GTP molecule at the dimer interface in the tetrameric enzyme leads to increased affinity for substrate ... "Allosteric regulation and substrate activation in cytosolic nucleotidase II from Legionella pneumophila". The FEBS Journal. 281 ...
... nucleotidase II (NT5C2) gene, while the TFDP3 gene has also been found to confer chemoresistance in children. Although over 100 ... 95% of T-Cell TCR consist of an alpha and beta chain (encoded by TRA and TRB, respectively), where only 5% of T-Cell TCR ... Most cases of leukemia increase with age, with ALL being the main exception, which peaks in children aged 2 to 5 years. T-ALL ... In childhood, T-ALL patients can expect a 5-year event free survival and overall survival of, respectively, 70% and 80%. ...
... nucleotidase activity in rat liver plasma membranes: Hepatic calcium-binding protein (regucalcin) reverses the Ca2+ effect". ... 36 (5): 1191-9. doi:10.3892/ijmm.2015.2343. PMID 26398287. Vaz CV, Correia S, Cardoso HJ, Figueira MI, Marques R, Maia CJ, ... 48 (5): 1955-1964. doi:10.3892/ijo.2016.3409. PMID 26935290. Cardosoa AL, Fernandes A, Aguilar-Pimentelc JA, de Angelisd MH, ... 327 (3): 251-5. doi:10.1016/0014-5793(93)80998-a. PMID 8348951. S2CID 1303220. Yamaguchi M, Makino R, Shimokawa N (December ...
5-Nucleotidase syndrome, rare (NIH) 5p minus syndrome 5q- syndrome 6 alpha mercaptopurine sensitivity, rare (NIH) 6-pyruvoyl- ... XXXXY syndrome 5 alpha reductase 2 deficiency 5-alpha-Oxoprolinase deficiency, rare (NIH) ...
Calcium-activated nucleotidase 1 (17q25.3) BIRC5: Survivin (17q25.3) CHMP6: Charged multivesicular body protein 6 (17q25.3) ... 11 (5): 206. doi:10.1186/gb-2010-11-5-206. PMC 2898077. PMID 20441615. "Statistics & Downloads for chromosome 17". HUGO Gene ... encoding protein 5-azacytidine-induced protein 1 BRCA1P1: encoding protein BRCA1 pseudogene 1 BCPR encoding protein Breast ... encoding enzyme Pyridoxine-5'-phosphate oxidase PPP1R27: encoding protein Protein phosphatase 1, regulatory subunit 27 PRCD: ...
... nucleotidases MeSH D08.811.277.352.650.600.600 - 5'-nucleotidase MeSH D08.811.277.352.650.620 - phosphatidate phosphatase MeSH ... map kinase kinase 5 MeSH D08.811.913.696.620.682.700.565.600 - map kinase kinase 6 MeSH D08.811.913.696.620.682.700.565.700 - ... map kinase kinase 5 MeSH D08.811.913.696.620.682.725.200.600 - map kinase kinase 6 MeSH D08.811.913.696.620.682.725.200.700 - ... proprotein convertase 5 MeSH D08.811.277.656.300.760.680 - pronase MeSH D08.811.277.656.300.760.733 - protease la MeSH D08.811. ...
... nucleotidase (5-NT) is a protein produced by the liver. A test can be done to measure the amount of this protein in your blood ... nucleotidase (5-NT) is a protein produced by the liver. A test can be done to measure the amount of this protein in your blood ...
Nucleotidase (5NT) is an intrinsic membrane glycoprotein that is present as an enzyme in a wide variety of mammalian cells. It ... facilitates the hydrolysis of the phosphate group from 5-nucleotides, resulting in corresponding nucleosides. ... Nucleotidase (5NT) is an intrinsic membrane glycoprotein that is present as an enzyme in a wide variety of mammalian cells. [1 ... Nucleotidase (5NT) is an intrinsic membrane glycoprotein that is present as an enzyme in a wide variety of mammalian cells. [1 ...
... nucleotidase, thimidine monophosphate nucleotidase, 5′-AMPase, 5′-AMP nucleotidase, AMP phosphohydrolase, IMP 5′-nucleotidase ... Reis, Julian (1934). "Nucleotidase and its relation to the deamination of nucleotides in the heart and the muscles". Bulletin ... nucleotidase activity can be used as an index of lead poisoning. It is believed that lead induced deficiency of the enzyme in ... Nucleotidase Levels Blood Test. Ecto-5Nucleotidase is considered a maturation marker for T cells and B cells. This is due to ...
This page includes the following topics and synonyms: 5 Nucleotidase.
analysis of Drosophila and human 7-methyl GMP-specific nucleotidases Title: Identification of Drosophila and human 7-methyl GMP ... nucleotidase, cytosolic IIIAprovided by HGNC. Primary source. HGNC:HGNC:17820 See related. Ensembl:ENSG00000122643 MIM:606224; ... nucleotidase deficiency: two cases report and literature review]. Li Y, et al. Zhonghua Xue Ye Xue Za Zhi, 2021 Aug 14. PMID ... nucleotidase 3. lupin. pyrimidine 5-nucleotidase 1. uridine 5-monophosphate hydrolase 1. NP_001002009.1. *EC 3.1.3.5 ...
... nucleotidase (CD73) in renal ischemia. J Am Soc Nephrol. 18:833-845. 2007. View Article : Google Scholar : PubMed/NCBI ... nucleotidase/CD73 in glioma cell line proliferation. Mol Cell Biochem. 319:61-68. 2008. View Article : Google Scholar : PubMed/ ... nucleotidase (eN, CD73) in cell lines from various stages of human melanoma. Melanoma Res. 16:213-222. 2006. View Article : ... nucleotidase and adenosine in breast cancer. Clin Cancer Res. 10:708-717. 2004. View Article : Google Scholar : PubMed/NCBI ...
... nucleotidase 1A in sporadic inclusion body myositis versus known autoimmune diseases ... nucleotidase 1A in sporadic inclusion body myositis versus known autoimmune diseases ... nucleotidase 1A in sporadic inclusion body myositis versus known autoimmune diseases ...
Nucleotidase (5NT) is an intrinsic membrane glycoprotein that is present as an enzyme in a wide variety of mammalian cells. It ... facilitates the hydrolysis of the phosphate group from 5-nucleotides, resulting in corresponding nucleosides. ... Nucleotidase (5NT) is an intrinsic membrane glycoprotein that is present as an enzyme in a wide variety of mammalian cells. [1 ... Nucleotidase (5NT) is an intrinsic membrane glycoprotein that is present as an enzyme in a wide variety of mammalian cells. [1 ...
Nucleotidase (5NT) is an intrinsic membrane glycoprotein that is present as an enzyme in a wide variety of mammalian cells. It ... facilitates the hydrolysis of the phosphate group from 5-nucleotides, resulting in corresponding nucleosides. ... Nucleotidase (5NT) is an intrinsic membrane glycoprotein that is present as an enzyme in a wide variety of mammalian cells. [1 ... Nucleotidase (5NT) is an intrinsic membrane glycoprotein that is present as an enzyme in a wide variety of mammalian cells. [1 ...
Nucleotidase (5NT) is a glycoprotein found throughout the body, in the cytoplasmic membrane, catalyzing the conversion to ... 45 (5): 558-568. PMC 4935699. PMID 27398328.. *^ a b Kasper, Dennis L.; Fauci, Anthony S.; Hauser, Stephen L.; Longo, Dan L.; ... Retrieved 5 August 2011.. *^ Johnston DE (1999). "Special considerations in interpreting liver function tests". Am Fam ... Pathological jaundice in newborns should be suspected when the serum bilirubin level rises by more than 5 mg/dL per day, serum ...
... nucleotidase, of which GGT and alkaline phosphatase are most widely used.[16] These enzymes are elevated in hepatobiliary ... nucleotidase. Cholestasis. GGT. Cholestasis; medications, ethanol; rarely anorexia nervosa, hyperthyroidism, myotonic dystrophy ... Laboratory tests for cholestasis include GGT, alkaline phosphatase, and 5′ ...
... nucleotidase e adenosina deaminase em soro de pacientes esquizofrênicos. dc.contributor.advisor. Belmonte-de-Abreu, Paulo Silva ... nucleotidase e adenosina deaminase em soro de pacientes esquizofrênicos. pt_BR. ... Sistema purinérgico em esquizofrenia : determinação da atividade das enzimas 5 ... Sistema purinérgico em esquizofrenia : determinação da atividade das enzimas 5 ...
... nucleotidase activity was found in the range 27-33°C and using a substrate concentration of ≥ 1·5 μmol AMP ml-1 for whole cells ... nucleotidase activity was found in the range 27-33°C and using a substrate concentration of ≥ 1·5 μmol AMP ml-1 for whole cells ... nucleotidase activity was found in the range 27-33°C and using a substrate concentration of ≥ 1·5 μmol AMP ml-1 for whole cells ... nucleotidase activity was found in the range 27-33°C and using a substrate concentration of ≥ 1·5 μmol AMP ml-1 for whole cells ...
... bisphosphate nucleotidase activity (Molecular function). Catalysis of the reaction: adenosine 3,5-bisphosphate + H2O = ...
... bisphosphate nucleotidase; DHC1, Dynein heavy chain; GPHN, Gephyrin, molybdenum cofactor biosynthesis protein; PCS, peroxisomal ... Primer sequence, 5′ → 3′†. G. destructans/G. pannorum GenBank accession nos.. ALR. Penicillium marneffei (XP_002152078.1). 654/ ...
Nucleotidase im Serum. J. BECKMANN, K. LEYBOLD, L. WEISBECKER ... Kinetische Bestimmung der 5-Nucleotidase im Serum. K. LEYBOLD ...
... nucleotidase, and the investigation of a number of physical and biochemical properties of the enzyme with the aim of comparing ... nucleotidase and members of the Ser/Thr protein phosphatase family in the catalytic site has lead to 5-nucleotidase being ... nucleotidase. This study suggests that the metal binding site, M2, that includes residues Asp-84, His-217 and His-252, is ... nucleotidase activity by particular divalent metal ions, this thesis describes a proposed model in which these divalent ions ...
Nucleotidase (CD73). Novel, small molecule nucleotide derivatives have been developed containing either a purine or pyrimidine ... 5 p.m.. ET, Monday - Friday Email: [email protected] Phone: +1-800-860-8747 TTY: +711 Chat. Live Chat Available. 8:30 a. ... nucleotidase. CD73 converts extracellular AMP to adenosine, the native activator… ... nucleobase that competitively block the enzyme CD73, or ecto-5- ...
Phenotype data for mouse gene Nt5el. Discover Nt5els significant phenotypes, expression, images, histopathology and more. Data for gene Nt5el is all freely available for download.
... nucleotidase) Antibody AD2 ... PE/Dazzle™ 594 anti-human CD73 (Ecto-5'-nucleotidase) ... APC/Fire™ 750 anti-human CD73 (Ecto-5'- ... PE/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase) ...
... nucleotidase (5NT) and erythrocyte activity of TPMT, key enzymes in thiopurine catabolism, were measured in 3 RA patients who ... 1995 Jan;38(1):142-5. doi: 10.1002/art.1780380122. Authors P J Kerstens 1 , J N Stolk, R A De Abreu, L H Lambooy, L B van de ... Methods: Lymphocyte activity of purine nucleoside phosphorylase and 5- ...
Nucleotidase. References. 4 Miscellaneous Enzymes in Human Cancer. I. Introduction. II. Amylase. III. Leucine Aminopeptidase. ... 5 The Neoplastic Immunoglobulinopathies. I. Introduction. II. Human Immunoglobulins. III. Immunoglobulins in Cancer. IV. ...
... nucleotidase. *total bile acids. *Other markers of cell changes or cellular function *albumin ... If the gavage vehicle is oil (see Chapter IV.B.5.b. in the 1993 draft "Redbook II"), then the volume should be no more than 0.4 ... A minimum of three dose levels of the test substance, per sex, should be used, however 4 or 5 dose levels of the test substance ... Related issues are discussed in the section on Diets for Toxicity Studies in Chapter IV.B.5. in the 1993 draft "Redbook II" ...
... nucleotidase (5N) (E.C.-3.1.3.5) is present in lymphocytes isolated from the blood of normal subjects. This activity is ... nucleotidase prompted studies of the effects of lead on this and other erythrocyte enzymes. In vitro incubations of normal ... nucleotidase occurred in the presence of lead at concentrations that had minimal effects on many other erythrocyte enzymes ... nucleotidase activity. Results suggest that lead-induced deficiency of this enzyme in maturing erythroid elements could, if ...
... nucleotidase (CD73) helps maintain endothelial barrier function. We tested whether interferon-beta-1a (IFN-beta-1a), which ... Production of anti-inflammatory adenosine by ecto-5′- ...
2,3-cyclic-nucleotide 2-phosphodiesterase / 3-nucleotidase / 5-nucleotidase [EC:3.1.4.16 3.1.3.6 3.1.3.5]. ... nucleotidase / UDP-sugar diphosphatase [EC:3.1.3.5 3.6.1.45]. K11753 riboflavin kinase / FMN adenylyltransferase [EC:2.7.1.26 ... mevalonate-3-phosphate-5-kinase [EC:2.7.1.186]. K18693 diacylglycerol diphosphate phosphatase / phosphatidate phosphatase [EC: ... IMP and pyridine-specific 5-nucleotidase [EC:3.1.3.99 3.1.3.-]. K18551 pyrimidine and pyridine-specific 5-nucleotidase [EC: ...
One antigen, one gold? A quantitative analysis of immunogold labeling of plasma membrane 5-nucleotidase in frozen thin ... One antigen, one gold? A quantitative analysis of immunogold labeling of plasma membrane 5-nucleotidase in frozen thin ...
... nucleotidase (P5N) in lead exposed workers: With focus on the effect on hemoglobin, Industrial Health, vol. 40, no. 1, pp. 23- ... nucleotidase (P5N) in lead exposed workers: With focus on the effect on hemoglobin. Industrial Health. 2002;40(1):23-27. https ... nucleotidase (P5N) in lead exposed workers : With focus on the effect on hemoglobin. / Kim, Yangho; Yoo, Cheol In; Lee, Choong ... nucleotidase (P5N) in lead exposed workers : With focus on the effect on hemoglobin. In: Industrial Health. 2002 ; Vol. 40, No ...
  • Wolff G, Truse R, Decking U. Extracellular Adenosine Formation by Ecto-5'-Nucleotidase (CD73) Is No Essential Trigger for Early Phase Ischemic Preconditioning. (medscape.com)
  • Alternative splicing of human NT5E in cirrhosis and hepatocellular carcinoma produces a negative regulator of ecto-5'-nucleotidase (CD73). (medscape.com)
  • Novel, small molecule nucleotide derivatives have been developed containing either a purine or pyrimidine nucleobase that competitively block the enzyme CD73, or ecto-5'-nucleotidase. (nih.gov)
  • Production of anti-inflammatory adenosine by ecto-5′-nucleotidase (CD73) helps maintain endothelial barrier function. (thelancet.com)
  • CD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. (biolegend.com)
  • CD73 is a 5'-nucleotidase anchored to the membrane by a glycosylphosphatidylinositol (GPI) structure. (beckman.com)
  • In addition, CD73, a 5'-nucleotidase, can be used to release the 5'-phosphate from 5'-AMP to increase the assay sensitivity by two-fold. (rndsystems.com)
  • The addition of the nucleotidase CD73 is optional. (rndsystems.com)
  • Also known as ecto-5' nucleotidase or 5'-nucleotidase, CD73 is an anchored cell surface protein that plays a critical role in purinergic signaling by catalyzing the hydrolysis of AMP into adenosine and phosphate. (the-scientist.com)
  • 2015). Deficits in Endogenous Adenosine Formation by Ecto-5′-Nucleotidase/CD73 Impair Neuromuscular Transmission and Immune Competence in Experimental Autoimmune Myasthenia Gravis . (up.pt)
  • Prior research has focused largely on the two major ectonucleotidases, ectonucleoside triphosphate diphosphohydrolase 1/cluster of differentiation (CD)39 and ecto-5'-nucleotidase/CD73, which catalyze the breakdown of extracellular ATP into adenosine, and on the subsequent activation of different subtypes of adenosine receptors with mixed findings of antitumor and protumor effects. (utu.fi)
  • Additionally, ≥ 95% of the MSCs population must positively express a cluster of differentiation superficial antigenic markers of hematopoietic differentiation called CDs (for cluster of differentiation) such as CD105 (endoglin), CD73 (ecto-5'-nuclease), and CD90 (Thy1), as measured by flow cytometry. (pocketdentistry.com)
  • CD73, also known as 5′-nucleotidase (5′-NT), is commonly used to convert amp to adenosine. (immune-system-research.com)
  • CD73, known as ecto-5′-nucleotidase (ecto-5′-NT, EC 3.1.3.5) is a glycosyl-phosphatidylinositol (GPI)-linked 70-kDa cell surface enzyme found in most tissues. (immune-system-research.com)
  • Ecto-5'-nucleotidase as a glycosylphosphatidylinositol-anchored membrane protein which is defined as the lymphocyte differentiation antigen (CD73). (azpdf.org)
  • The NT5E (CD73) molecule represents an ecto-5'-nucleotidase expressed on the cell surface of various cell types. (shengsci.com)
  • Holmes AP, Nunes AR, Cann MJ, Kumar P. Ecto-5'-Nucleotidase, Adenosine and Transmembrane Adenylyl Cyclase Signalling Regulate Basal Carotid Body Chemoafferent Outflow and Establish the Sensitivity to Hypercapnia. (medscape.com)
  • In mammalian cells the enzyme is predominantly located in the plasma membrane and its primary role is in the conversion of extracellular nucleotides (e.g. 5'-AMP), which are generally impermeable, to the corresponding nucleoside (e.g. adenosine) which can readily enter most cells. (wikipedia.org)
  • Catalysis of the reaction: adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate. (ntu.edu.sg)
  • 8,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-monophosphate to phosphate per minute per minute at pH 7.5 at 25°C. (prospecbio.com)
  • In this method, Golgi-resident PAP-specific 3'-phosphatase (gPAPP) is used to release the 3'-phosphate from PAP, generating 5'-adenosine monophosphate (5'-AMP). (rndsystems.com)
  • 8-Butylthio-2', 3'-isopropylidene − adenosineβ, γ -methylene 5'-triphosphate (14 ) 8-Butylthio-2′,3′-isopropylidene-adenosine (13) (100 mg, 0.25 mmol) was dissolved in dry trimethyl phosphate (1.5 mL), cooled to -15°C, and proton sponge was added (178.6 mg, 0.83 mmol, 3.3 eq). (dadospdf.com)
  • Adenosine A2A Receptor Agonist, 2-p-(2-Carboxyethyl)phenethylamino-5′-N-ethylcarboxamidoadenosine hydrochloride hydrate (CGS21680), Inhibits Inflammation and Increases Fibroblast Growth Factor 2 (FGF-2) Tissue Expression in Carrageenan Induced Rat Paw Edema. (unina.it)
  • Adenosine is the final product of ATP metabolism, mainly derived from the action of 5'- nucleotidase cleavage of AMP. (unina.it)
  • The purpose of the present work was to investigate on the antinflammatory effect of systemic administration of the adenosine A2A agonist, 2-p-(2- Carboxyethyl)phenethylamino-5′-N-ethylcarboxamidoadenosine hydrochloride hydrate (CGS21680), in the rat model of carrageenan-induced paw edema. (unina.it)
  • Results: Analgesia and increased ecto-5'-nucleotidase expression and adenosine content in the spinal cord were observed 1 h after electroacupuncture. (scielo.org)
  • α,β-methyleneadenosine 5'-diphosphate was able to inhibit upregulation of adenosine content and electroacupuncture-induced analgesia. (scielo.org)
  • Conclusions: Our results suggest that electroacupuncture at Zusanli can produce analgesia in chronic constriction injury rat models, possibly via the increased ecto-5'-nucleotidase expression induced through electroacupuncture, thus leading to increased adenosine expression in the spinal cord. (scielo.org)
  • In addition, released ATP may be dephosphorylated by extracellular nucleotidases to form endogenous adenosine sequentially. (kw-6002.info)
  • Ecto-5-nucleotidase (Ecto-5-NTase), the limiting enzyme of the ectonucleotidase pathway, plays an important role in regulating the rate of local adenosine production from adenine nucleotides. (kw-6002.info)
  • The failure of ecto-5-nucleotidase inhibitors to modify [3H]ACh release during brief stimulation trains contrasts with the facilitatory effect of the exogenously added adenosine precursor AMP. (kw-6002.info)
  • Alternatively, the postsynaptic localization of ecto-5-nucleotidase (Nitahara and models suggest that the balance between inhibitory adenosine A1 and facilitatory A2A receptors may be important in regulating intestinal motility. (kw-6002.info)
  • Removing a phosphate group via nucleotidase to form adenosine followed by deamination (removal of a -NH2 amino group) to form inosine. (tigerfitness.com)
  • 5'-nucleotidase, cytosolic II genotype, and clinical outcome in patients with acute myeloid leukemia with intermediate-risk cytogenetics. (cdc.gov)
  • Genetic variants in cytosolic 5'-nucleotidase II are associated with its expression and cytarabine sensitivity in HapMap cell lines and in patients with acute myeloid leukemia. (cdc.gov)
  • Cytosolic 5′-Nucleotidase 1A As a Target of Circulating Autoantibodies in Autoimmune Diseases" Arthritis Care & Research 68(1): 66-71 (2015). (abmgood.com)
  • Numerous studies have shown that erythrocyte pyrimidine 5′-nucleotidase activity is significantly lowered in patients with lead poisoning, and that pyrimidine 5′-nucleotidase activity can be used as an index of lead poisoning. (wikipedia.org)
  • The encoded protein is the type 1 isozyme of pyrimidine 5' nucleotidase and catalyzes the dephosphorylation of pyrimidine 5' monophosphates. (nih.gov)
  • A) in NT5C3A associated with hereditary pyrimidine 5'-nucleotidase deficiency: two cases report and literature review]. (nih.gov)
  • A New Homozygous Mutation (c.393-394del TA/c.393-394del TA) in the NT5C3 Gene Associated With Pyrimidine-5'-Nucleotidase Deficiency: A Case Report. (nih.gov)
  • The intracellular pyrimidine 5'-nucleotidase NT5C3A is a negative epigenetic factor in interferon and cytokine signaling. (nih.gov)
  • Lead also increases the rate of red blood cell destruction due to the profoundly depressed activities of erythrocyte pyrimidine 5′-nucleotidase (P5N) activities. (elsevier.com)
  • 5'-nucleotidase (5'-NT) is a protein produced by the liver. (medlineplus.gov)
  • Ecto‑5'‑nucleotidase, also termed cluster of differentiation (CD)73, is a protein that is activated in several types of aggressive cancer and may promote cancer progression. (spandidos-publications.com)
  • Ecto-5′-nucleotidase, also termed cluster of differentiation (CD)73, is a glycosyl phosphatidylinositol-linked membrane protein found on the surface of a variety of cell types ( 14 ). (spandidos-publications.com)
  • Significant sequence homology between the E. coli 5'-nucleotidase and members of the Ser/Thr protein phosphatase family in the catalytic site has lead to 5'-nucleotidase being included in this protein family. (edu.au)
  • The molecular weight of the mature protein was estimated as 58219 daltons, with a specific activity for 5'-AMP, in the presence of 4 mM Co2+ and 13 mM Ca2+ at pH 6.0, of 730 mmol/min/mg. (edu.au)
  • Seven key catalytic site residues (Asp-41, His-43, Asp-84, His-117, Glu-118, His-217 and His-252) were selected on the basis of sequence conservation within the Ser/Thr protein phosphatases and 5'-nucleotidases. (edu.au)
  • Is predicted to encode a protein with the following domain: Warthog protein 3/5. (wormbase.org)
  • 5-hydroxytryptamine, ATP and Glu act through both G protein-coupled receptors (GPCRs), and ionotropic receptors, i.e., ligand gated ion channels. (frontiersin.org)
  • 5-HT, Glu and ATP, taken as regulatory molecules, share being the endogenous agonists of two types of cell surface receptors: G protein-coupled receptors (GPCRs) and ionotropic receptors. (frontiersin.org)
  • indicates that the protein contains two domains (at least), one of which performs the role Glutamate 5-kinase and the other which performs the role RNA-binding C-terminal domain PUA . (bv-brc.org)
  • Laminin-332 (laminin-5) is a basement membrane heterotrimeric protein composed of alpha-3, beta-3 and gamma-2 laminin chains. (morulaivf.com)
  • Biomolecule/Target: NT5C3B Alternates names: NT5C3L, 7-methylguanosine phosphate-specific 5'-nucleotidase Synonyms: NT5C3L, 7-methylguanosine phosphate-specific. (telospub.com)
  • Serum levels of 5′-nucleotidase in disease. (medscape.com)
  • Pathological jaundice in newborns should be suspected when the serum bilirubin level rises by more than 5 mg/dL per day, serum bilirubin more than the physiological range, clinical jaundice more than 2 weeks, and conjugated bilirubin (dark urine staining clothes). (wikipedia.org)
  • The serum alkaline phosphatase (AP) is typically elevated four to five times the upper limit of normal, but elevations up to 20 times the pediatric upper reference limit (or 50 times the adult upper reference limit) have been described [ 5-7 ]. (houstonwehaveaproblemblog.com)
  • Laboratory tests for cholestasis include GGT, alkaline phosphatase, and 5′ nucleotidase, of which GGT and alkaline phosphatase are most widely used. (medscape.com)
  • However, acid and alkaline phosphatase, NADH oxidase and 5′-nucleotidase showed no such specific location. (microbiologyresearch.org)
  • In addition to AST and ALT, alkaline phosphatase, 5' nucleotidase, and gamma-glutamyl transpeptidase (GGT) are a few of the other enzymes located in the liver. (vaccinehealthcenter.com)
  • 5′-Nucleotidase (5NT) is an intrinsic membrane glycoprotein that is present as an enzyme in a wide variety of mammalian cells. (medscape.com)
  • 5′-Nucleotidase (EC 3.1.3.5) is an enzyme which catalyzes the phosphorylytic cleavage of 5′-nucleotides. (wikipedia.org)
  • The enzyme has a wide substrate specificity for nucleotides and has been shown to hydrolyze 5'nucleotides rapidly, ribose-5-phosphate slowly, and other phosphate esters extremely slowly (if at all). (wikipedia.org)
  • Studies of mammalian 5′-nucleotidases have shown that there exist at least four different forms of the 5′-nucleotidase enzyme: one membrane-bound form and three soluble forms. (wikipedia.org)
  • This is due to the fact that the enzyme activity is approximately 10-times higher for peripheral T cells than thymocytes, 5-6 times higher in adult peripheral B cells than fetal B cells, and largely absent in non-T cell and non-B cell lymphocytes. (wikipedia.org)
  • MAF-treated cells were also found to lose 5′ nucleotidase activity faster than control cells in the presence of cycloheximide, suggesting such cells may have a higher membrane turnover of this enzyme. (elsevier.com)
  • Escherichia coli 5'-nucleotidase is a periplasmically localised enzyme capable of hydrolysing a broad range of substrates, including all 5'-ribo- and 5'-deoxyribonucleotides, uridine diphosphate sugars, and a number of synthetic substrates such as bis (r-nitrophenyl) phosphate. (edu.au)
  • This thesis describes the development of a rapid purification methodology for milligram quantities of 5'-nucleotidase, and the investigation of a number of physical and biochemical properties of the enzyme with the aim of comparing these properties to those of certain catalytic site mutants. (edu.au)
  • Three plasma membrane-located enzyme activities were analysed: Mg2+-dependent ATPase, 5´-nucleotidase and adenylyl cyclase. (lu.se)
  • This gene encodes a member of the 5'-nucleotidase family of enzymes that catalyze the dephosphorylation of nucleoside 5'-monophosphates. (nih.gov)
  • Lymphocyte activity of purine nucleoside phosphorylase and 5'-nucleotidase (5NT) and erythrocyte activity of TPMT, key enzymes in thiopurine catabolism, were measured in 3 RA patients who had experienced AZA-related bone marrow toxicity and in 16 RA patients without signs of toxicity despite at least 6 months of treatment with AZA. (nih.gov)
  • Nucleoside kinases catalyse the reverse reaction to 5-nucleotidase, and these reverse reactions have been suggested for almost all the nucleotideCnucleoside pairs [11]. (lde225.info)
  • mRNAs were amplified and applied to a Focused DNA Array, which was restricted to well-known genes, including GEM sensitivity-related genes, deoxycytidine kinase (dCK), human equilibrative nucleoside transporter 1 (hENT1), hENT2, dCMP deaminase, cytidine deaminase, 5'-nucleotidase, ribonucleotide reductase 1 (RRM1) and RRM2. (fujita-hu.ac.jp)
  • The role of ecto-5'-nucleotidase in endothelial dysfunction and vascular pathologies. (medscape.com)
  • Studies of the soluble form of human ecto-5′-nucleotidase, without a GPI anchor, have shown that the C-terminal domain holds the substrate-binding pocket, and that the aromatic purine motif of the substrate is stacked between two phenylalanine residues. (wikipedia.org)
  • The test may also be referred to as 5'NT Levels Blood Test, CDF73 Levels Blood Test, and Ecto-5'-Nucleotidase Levels Blood Test. (wikipedia.org)
  • Physiopathological role of ecto-5`-nucleotidase - a new target for neuroprotection. (fct.pt)
  • Objective: This study aimed to determine the role that ecto-5'-nucleotidase plays in EA-induced analgesia for neuropathic pain. (scielo.org)
  • We observed the mechanical withdrawal threshold and thermal pain threshold and detected the expression of ecto-5'-nucleotidase in the spinal cord using Western blot. (scielo.org)
  • Chronic constriction injury rat models were intraperitoneally injected with α,β-methyleneadenosine 5'-diphosphate, an ecto-5'-nucleotidase inhibitor, 30 min before electroacupuncture. (scielo.org)
  • Objetivo: Este estudo teve como objetivo determinar o papel que a ecto-5'-nucleotidase desempenha na analgesia induzida por EA para dor neuropática. (scielo.org)
  • Observamos o limiar de retirada mecânica e o limiar de dor térmica, e detectamos a expressão de ecto-5'-nucleotidase na medula espinhal usando Western blot. (scielo.org)
  • Modelos de ratos com lesão de constrição crônica foram injetados intraperitonealmente com α, β-metilenadenosina 5'-difosfato, um inibidor de ecto-5'-nucleotidase, 30 min antes da eletroacupuntura. (scielo.org)
  • Resultados: Analgesia e aumento da expressão de ecto-5'-nucleotidase e conteúdo de adenosina na medula espinhal foram observados 1 h após a eletroacupuntura. (scielo.org)
  • Conclusões: Nossos resultados sugerem que a eletroacupuntura em Zusanli pode produzir analgesia em modelos de ratos com lesão de constrição crônica, possivelmente por meio do aumento da expressão de ecto-5'-nucleotidase induzida por eletroacupuntura, levando ao aumento da expressão de adenosina na medula espinhal. (scielo.org)
  • Gustaffson induced by electrical stimulation (Begg the ecto-nucleotidase pathway activates facilitatory A2A receptors in a time-dependent manner. (kw-6002.info)
  • One of the soluble forms appears to be derived from the GPI-anchored ex-5'-nucleotidase and has an extracellular location. (wikipedia.org)
  • Mutations in this gene are a cause of hemolytic anemia due to uridine 5-prime monophosphate hydrolase deficiency. (nih.gov)
  • Nucleotidase which shows specific activity towards cytidine monophosphate (CMP) and 7-methylguanosine monophosphate (m(7)GMP) (PubMed:24603684). (icr.ac.uk)
  • Guanosine 3',5'-cyclic monophosphate (cyclic GMP or cGMP) is a second messenger that is biosynthesized from GTP by guanylate cyclases. (glpbio.cn)
  • Sulfotransferases are a large group of enzymes that transfer sulfate from the donor substrate 3'-phosphoadenosine-5'-phosphosulfate (PAPS) to various acceptor substrates, generating 3'-phosphoadenosine-5'-phosphate (PAP) as a by-product. (rndsystems.com)
  • We have decided to put into perspective three examples with their analogies and differences, namely 5-hydroxytryptamine (5-HT), glutamate (Glu) and ATP. (frontiersin.org)
  • This is then followed by dephosphorylation (removal of a phosphate group) by a nucleotidase to form inosine. (tigerfitness.com)
  • Polyclonal and monoclonal antibodies against chicken gizzard 5 '-nucleotidase inhibit the spreading process of chicken embryonic fibroblasts on laminin substratum. (morulaivf.com)
  • Polyclonal and monoclonal antibodies raised against chicken gizzard 5′-nucleotidase were tested in adhesion assays of embryonic chicken fibroblasts (CEF) for their ability to interfere with the adhesion process of these cells on either laminin or fibronectin substrata. (morulaivf.com)
  • The initial attachment process of CEF on fibronectin and laminin substrata was not influenced by preincubating these cells with antibodies against chicken gizzard 5′-nucleotidase. (morulaivf.com)
  • av R De la Rosa · 2019 · Citerat av 3 - For cDNA synthesis, 1 µg of total RNA was digested with DNase and incubated at 37 °C for 30 min, and 1 µL of 5 mM EDTA was then added to stop reaction the first reaction, age group, gestation period when reaction/event was observed females in the last third of gestation, non-castrated bulls and bovines shipped Breed or mix between? (web.app)
  • In vitro assays demonstrated a competition of laminin and this monoclonal antibody for the binding site on purified 5′-nucleotidase. (morulaivf.com)
  • relationship between the active sites of 2',3'-cyclic phosphodiesterase with 3'-nucleotidase activity purified from vibrio alginolyticus. (liverpool.ac.uk)
  • To better understand the complex family of nucleotidases in vegetation, we have cloned and characterised the gene encoding a new nucleotidase member of the HAD superfamily in sequence (Phvul.004G174200) was conducted in the NCBI database in the genome [24]. (lde225.info)
  • KEY RESULTS The compounds 8-BuS-AMP, 8-BuS-ADP and 8-BuS-ATP inhibit recombinant human and mouse NTPDase1 by mixed type inhibition, predominantly competitive with Ki values 95% purity) using solvent system II (60 mM ammonium phosphate (A) and 5 mM TBAP in 90% water/10% methanol (B), applying a gradient of 25% A to 75% A in 20 min), tR = 6 min (>97% purity). (dadospdf.com)
  • A novel method for analysing 5′ nucleotidase activity of rainbow trout, Oncorhynchus mykiss, head kidney macrophages was optimised for use with cell monolayers, with respect to the effect of cell numbers, temperature and substrate concentration. (elsevier.com)
  • Maximal 5′ nucleotidase activity was found in the range 27-33°C and using a substrate concentration of ≥ 1·5 μmol AMP ml -1 for whole cells and ≥1·5 μmol AMP ml -1 for lysed cells. (elsevier.com)
  • Concanavalin A inhibits the interaction of snake venom 5'-nucleotidase and actin. (mpg.de)
  • [ 1 ] It facilitates the hydrolysis of the phosphate group from 5'-nucleotides, resulting in corresponding nucleosides. (medscape.com)
  • The first step in the catabolic pathway is the removal of the 5-phosphate group catalysed by a phosphatase that hydrolyses the nucleotides into nucleosides. (lde225.info)
  • In most cases there are elevations in both liver and bone isoenzymes, and (rarely) in intestinal isoenzymes [ 5,8 ]. (houstonwehaveaproblemblog.com)
  • Molecular cloning and biochemical characterization of a 3'(2'),5'-bisphosphate nucleotidase from Debaryomyces hansenii. (princeton.edu)
  • Novel 3'-phosphoadenosine-5'-phosphatases from extremely halotolerant Hortaea werneckii reveal insight into molecular determinants of salt tolerance of black yeasts. (princeton.edu)
  • 5'-Nucleotidase activity is significantly stimulated by the addition of particular divalent metal ions, most notably cobalt which results in a 30-50 fold increase in activity. (edu.au)
  • Although the compound has been studied extensively for its neuronal handling and synaptic actions, serotonin 5-HT receptors can be found extra-synaptically and not only in neurons but in many types of mammalian cells, inside and outside the central nervous system (CNS). (frontiersin.org)
  • Compositional properties of 5'-nucleotidase SurE (bottom) versus UniprotKB/SwissProt (top). (ucy.ac.cy)
  • The clinical biochemistry of 5′-nucleotidase. (medscape.com)
  • The seven selected residues were modified by site-directed mutagenesis, and the effect of the amino acid substitutions upon the kinetic properties of 5'-nucleotidase activity was determined. (edu.au)
  • anion-activated 5'-nucleotidase in cell envelopes of a slightly halophilic vibrio alginolyticus. (liverpool.ac.uk)