Nucleotidases

5'-Nucleotidase

Apyrase

Cytidine Monophosphate

Haloferax volcanii

Pyrimidine Nucleotides

Steatitis

Anemia, Hemolytic

Uridine Monophosphate

Adenosine Monophosphate

Phosphoric Monoester Hydrolases

Adenosine Triphosphatases

Pyrophosphatases

Adenosine

Kinetics

Adenosine Diphosphate

Archaeal Proteins

Adenosine Triphosphate

Substrate Specificity

Erythrocytes

Nucleotides

Proteasome Endopeptidase Complex

Molecular Sequence Data

Microscopy, Electron

Hydrogen-Ion Concentration

Cell Membrane

Amino Acid Sequence

Molecular Weight

Electrophoresis, Polyacrylamide Gel

Liver

Mutation

Encyclopedias as Topic

Vertebrates

Phosphodiesterase I

Snake Venoms

Databases, Protein

Internet

User-Computer Interface

Software

Archives

USSR

Influenza A Virus, H2N2 Subtype

Retina

Russia

Biotin

Glycosylphosphatidylinositols

Antibodies, Monoclonal

Antigens, CD3

Metabolome

Metabolomics

Receptors, Purinergic P2Y1

Angiolymphoid Hyperplasia with Eosinophilia

Inclusion body myositis

NT5C1A

NT5E

NT5C

Herbert Zimmermann (neuroscientist)

Kuhl's maskray

NT5C3

Nucleic acid metabolism

BAY 60-6583

Ornithodoros savignyi

Tubuloglomerular feedback

Alkaline phosphatase

NT5M

Basophilic stippling

Lead(II) nitrate

Phosphatase

Annexin A2

Laminin, alpha 1

Purinergic signalling

Legionella pneumophila

Nucleotide salvage

Cholestasis

Cladribine

Hemolytic anemia

GUK1

DGUOK

SurE, survival protein E

5'-acylphosphoadenosine hydrolase

Thymidylate 5'-phosphatase

Periplasm

Proteasome

Spheroplast

5'-Nukleotidaza

Hormone-sensitive lipase

Allosteric regulation

Glucose 6-phosphatase

PETase

Phosphoserine phosphatase

CD38

P2RY10

Chromosome 17

PTEN (جين) - ويكيبيديا، الموسوعة الحرة

Allosteric regulation

Nukleotidaza

PLCG1

Eosinophil-derived neurotoxin

Phosphodiesterase 2

Restriction enzyme

Đái Duy Ban

Carol Remmer Angle

Mir-137

Fluorodeoxyuridylate

Purine metabolism

Pyrimidine21

• Novel, small molecule nucleotide derivatives have been developed containing either a purine or pyrimidine nucleobase that competitively block the enzyme CD73, or ecto-5'-nucleotidase. (nih.gov)
• The encoded protein is the type 1 isozyme of pyrimidine 5' nucleotidase and catalyzes the dephosphorylation of pyrimidine 5' monophosphates. (nih.gov)
• A New Homozygous Mutation (c.393-394del TA/c.393-394del TA) in the NT5C3 Gene Associated With Pyrimidine-5'-Nucleotidase Deficiency: A Case Report. (nih.gov)
• The intracellular pyrimidine 5'-nucleotidase NT5C3A is a negative epigenetic factor in interferon and cytokine signaling. (nih.gov)
• Pyrimidine-5'-nucleotidase Campinas, a new mutation (p.R56G) in the NT5C3 gene associated with pyrimidine-5'-nucleotidase type I deficiency and influence of Gilbert's Syndrome on clinical expression. (nih.gov)
• New missense homozygous mutation (Q270Ter) in the pyrimidine 5' nucleotidase type I-related gene in two Indian families with hereditary non-spherocytic hemolytic anemia. (nih.gov)
• Hemolytic anemia caused by pyrimidine 5'-nucleotidase (P5N) deficiency 15 years later. (biomedsearch.com)
• Structure of pyrimidine 5'-nucleotidase type 1. (nih.gov)
• Eukaryotic pyrimidine 5'-nucleotidase type 1 (P5N-1) catalyzes dephosphorylation of pyrimidine 5'-mononucleotides. (nih.gov)
• s case of pyrimidine 5'-nucleotidase deficiency and heterozygous [alpha]-thalassemia was an interesting co-existence (1). (thefreedictionary.com)
• Co-Existence of Hereditary Pyrimidine 5'-Nucleotidase Deficiency and Heterozygous a-Thalassemia: A Case Presentation. (thefreedictionary.com)
• A case of hemolytic anemia due to erythrocyte pyrimidine 5'-nucleotidase deficiency. (thefreedictionary.com)
• Detection of pyrimidine 5'-nucleotidase deficiency using [sup. (thefreedictionary.com)
• Lead inhibits heme biosynthesis and causes anemia, basophilic stippling, a decline in red cell 8-aminolevulinic acid dehydrogenase, and an increase in urinary 8-aminolevulinic acid, urinary coproporphyrin, red cell zinc protoporphyrin (ZPP), and pyrimidine 5'-nucleotidase (12). (thefreedictionary.com)
• The pyrimidine mononucleotides can be dephosphorylated by pyrimidine 5'-nucleotidase (P5N-I). In humans, a lack of this enzyme causes hemolytic anemia with ribosomal structures and trinucleotides retained in the red blood cells (RBCs). (uni-regensburg.de)
• Distribution of erythrocyte nucleotides in pyrimidine 5'-nucleotidase deficiency. (wizdom.ai)
• May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. (nih.gov)
• We present 2 Norwegian siblings who we believe to be the first cases of pyrimidine 5'-nucleotidase deficiency diagnosed in the Scandinavian countries. (symptoma.com)
• Abstract Two siblings with chronic hemolytic anemia due to red cell pyrimidine-5'-nucleotidase (P-5'-N) deficiency, presented within a few days of each other with a febrile illness and pancytopenia. (symptoma.com)
• mdN and cdN are mitochondrial and cytosolic 5'-3' pyrimidine nucleotidases. (thefullwiki.org)
• cN-III is a pyrimidine 5' nucleotidase. (thefullwiki.org)

NT5E6

• Alternative splicing of human NT5E in cirrhosis and hepatocellular carcinoma produces a negative regulator of ecto-5'-nucleotidase (CD73). (medscape.com)
• 5' Nucleotidase (Ecto 5' Nucleotidase or CD73 or NT5E or EC 3.1.3.5) pipeline Target constitutes close to 22 molecules. (marketresearch.com)
• The latest report 5' Nucleotidase - Pipeline Review, H1 2019, outlays comprehensive information on the 5' Nucleotidase (Ecto 5' Nucleotidase or CD73 or NT5E or EC 3.1.3.5) targeted therapeutics, complete with analysis by indications, stage of development, mechanism of action (MoA), route of administration (RoA) and molecule type. (marketresearch.com)
• Furthermore, this report also reviews key players involved in 5' Nucleotidase (Ecto 5' Nucleotidase or CD73 or NT5E or EC 3.1.3.5) targeted therapeutics development with respective active and dormant or discontinued projects. (marketresearch.com)
• We identified a cell surface ecto-5′-nucleotidase (Nt5e), as a candidate virulence factor. (umn.edu)
• Ecto-5' -Nucleotidase CD73 (NT5E), vitamin D receptor and FGF23 gene polymorphisms may play a role in the development of calcific uremic arteriolop. (cdc.gov)

Ecto28

• Gutensohn W., Thiel E., Buschette S. (1984) Ecto-5′-Nucleotidase as a Leukemia Marker. (springer.com)
• The test may also be referred to as 5'NT Levels Blood Test, CDF73 Levels Blood Test, and Ecto-5'-Nucleotidase Levels Blood Test. (wikipedia.org)
• The role of ecto-5'-nucleotidase in endothelial dysfunction and vascular pathologies. (medscape.com)
• Holmes AP, Nunes AR, Cann MJ, Kumar P. Ecto-5'-Nucleotidase, Adenosine and Transmembrane Adenylyl Cyclase Signalling Regulate Basal Carotid Body Chemoafferent Outflow and Establish the Sensitivity to Hypercapnia. (medscape.com)
• Wolff G, Truse R, Decking U. Extracellular Adenosine Formation by Ecto-5'-Nucleotidase (CD73) Is No Essential Trigger for Early Phase Ischemic Preconditioning. (medscape.com)
• Fluvastatin, an HMG-CoA reductase inhibitor, facilitate adenosine production in the rat hearts via activation of ecto-5'-nucleotidase. (nih.gov)
• Increased levels of adenosine and ecto 5'-nucleotidase (CD73) activity precede renal alterations in experimental diabetic rats. (nih.gov)
• Fluvastatin increases adenosine concentrations in the heart ventricles as a result of activation of PKC, mediated by stimulation of alpha1-adrenoceptors, through activation of ecto-5'-nucleotidase. (nih.gov)
• CD73, an ecto-5-prime-nucleotidase, is a glycosyl phophatidylinositol-anchored ectoenzyme that forms a disulfide-linked homodimer. (rndsystems.com)
• The AD2 antibody reacts with human CD73, a glycosyl phosphatidylinositol (GPI)-anchored glycoprotein and ecto-5'-nucleotidase expressed on the surface of subsets of B and T cells, follicular dendritic cells, mesenchymal stem cells, endothelial cells, and epithelial cells. (stemcell.com)
• To determine whether adenosine formation depends on extracellular nucleotide hydrolysis, we studied NaCl-dependent GFR regulation (tubuloglomerular feedback) in mice with targeted deletion of ecto-5′-nucleotidase/CD73 (e-5′NT/CD73), the enzyme responsible for adenosine formation from AMP. (jci.org)
• Here, we have used mice that lack the CD73 gene (encoding ecto-5′-nucleotidase that converts AMP to adenosine) to test whether action potential-dependent adenosine release in the cerebellum depends on prior ATP release. (jneurosci.org)
• Here, we have used mice that lack ecto-5′-nucleotidase ( CD73 ), a key enzyme required for the conversion of ATP to adenosine, to determine the mechanism of adenosine release and whether it arises from prior release of ATP. (jneurosci.org)
• The purpose of the present study was to evaluate the role of ecto-5'-nucleotidase (eN, ecto-5-NT, CD73) generated extracellular adenosine in biologically malignant behaviors of human breast cancer cell lines. (nih.gov)
• The generation of extracellular adenosine involves phosphohydrolysis of adenine nucleotide intermediates, and is regulated by the terminal enzymatic step catalyzed by ecto-5′-nucleotidase (CD73). (rupress.org)
• Ecto-5′-nucleotidase (5′-NT, CD73) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates ( 14 ). (rupress.org)
• CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. (biolegend.com)
• Several new biomarkers were identified with altered expression in aneuploid cells, including overexpression of the cellular prion protein CD230/PrP C and the immunosuppressive cell surface enzyme ecto-5′-nucleotidase CD73. (aacrjournals.org)
• Two main enzymes, cytosolic 5'-nucleotidase II (cN-II) and ecto-5'-nucleotidase (CD73) have been identified as main therapeutic targets in cancer. (chemaxon.com)
• Ecto-5′-nucleotidase (CD73) is an immunosuppressive molecule intricately involved in adaptive and innate immune responses and is able to dephosphorylate AMP to adenosine. (biomedcentral.com)
• This specific enzyme function has evolved in blood-feeding parasites from the ecto-ATPdase/CD39 (E-NTPDases)-, Cimex-type- and 5'-nucleotidase/CD73 enzyme families. (up.ac.za)
• Molecular modelling of these two proteins showed a similar protein structure to a periplasmic ecto-5'-nucleotidase from E. coli. (up.ac.za)
• Ecto-5'-nucleotidase (E5'N) is an endothelial surface enzyme that controls conversion of extracellular nucleotides into immunosuppressive adenosine. (unimib.it)
• The involvement of ecto-5′-nucleotidase (E-5′Nu) in the elevation of extracellular adenosine during inflammation is unclear. (hku.hk)
• To investigate the role of adenosine formed extracellularly in vascular homeostasis, mice with a targeted deletion of the cd73/ecto-5′- nucleotidase were generated. (elsevier.com)
• Ectonucleotidases tested were ectonucleoside triphosphate diphosphohydrolase 1-3 (NTPDase1-3) and ecto-5-nucleotidase (eN), whereas the effects were evaluated in two brain areas that show different sensitivity to glucocorticoid action, hippocampus, and cerebral cortex. (ac.rs)
• We investigated 10 target genes using a tagging SNP approach: the genes encoding CD73/ ecto-5'-nucleotidase (purinergic pathway), Matrix Gla protein, Fetuin A, Bone Gla protein, VKORC1 (all related to intrinsic calcification inhibition), calcium-sensing receptor, FGF23, Klotho, vitamin D receptor, stanniocalcin 1 (all related to CKD-MBD). (cdc.gov)
• The last and rate-limiting step of the hydrolysis is catalyzed by membrane-bound ecto-50-nucleotidase (eN). (ac.rs)

Deficiency5

• Mutations in this gene are a cause of hemolytic anemia due to uridine 5-prime monophosphate hydrolase deficiency. (nih.gov)
• In lead poisoning, anemia, basophilic stippling and inhibition of erythrocyte pyrimidin 5' deficiency are also observed. (biomedsearch.com)
• In the present work, we report two cases of hemolytic anemia secondary to congenital deficiency of pyrimidin 5' nucleotidase and another case secondary to lead poisoning. (biomedsearch.com)
• Since 1974, when pyrimidin 5' nucleotidase deficiency was isolated, is known that hemolysis is related to the accumulation of pyrimidin nucleotides within the erythrocytes that behave as metabolic inhibitors. (biomedsearch.com)
• 5'-Nucleotidase activity in retinol deficiency induced albino rats. (bvsalud.org)

Dephosphorylation6

• This gene encodes a member of the 5'-nucleotidase family of enzymes that catalyze the dephosphorylation of nucleoside 5'-monophosphates. (nih.gov)
• We conclude that the generation of adenosine at the glomerular pole depends to a major extent on e-5′NT/CD73-mediated dephosphorylation of 5′-AMP, presumably generated from released ATP. (jci.org)
• Their dephosphorylation by 5'NTs might be one of the mechanisms behind the resistance experienced by patients towards such drugs.This thesis describes structure-function studies on four of the seven known human 5'-NTs. (openthesis.org)
• However, cytoplasmic 5′-nucleotidases catalyze dephosphorylation of ara-CMP, thereby reducing the amount of ara-C for conversion to ara-CTP ( Amici and Magni, 2002 ). (aspetjournals.org)
• also known as cN-II and high K m 5′-nucleotidase) and cytoplasmic 5′-nucleotidase III (NT5C3) are involved in the final step of dephosphorylation before export of nucleosides out of the cell. (aspetjournals.org)
• This gene encodes a nucleotidase that catalyzes the dephosphorylation of the 5' deoxyribonucleotides (dNTP) and 2'(3')-dNTP and ribonucleotides, but not 5' ribonucleotides. (genecards.org)

Nucleoside6

• In mammalian cells the enzyme is predominantly located in the plasma membrane and its primary role is in the conversion of extracellular nucleotides (e.g. 5'-AMP), which are generally impermeable, to the corresponding nucleoside (e.g. adenosine) which can readily enter most cells. (wikipedia.org)
• The 5-prime-nucleotidase activity of CD73 converts extracellular nucleoside 5-prime monophosphates to nucleosides. (rndsystems.com)
• CD73 catalyzes the hydrolysis of 5'-adenosine monophosphate (AMP) to form the bioactive nucleoside adenosine, and plays a pivotal role in the activation of P1 adenosine receptors by regulating extracellular adenosine concentrations. (stemcell.com)
• In humans, eight 5'-nucleotidases catalyze the hydrolysis of nucleoside 5'-monophosphates into their respective nucleosides in order to regulate the intracellular / extracellular nucleotide pools. (chemaxon.com)
• Taking advantage of complete genome sequencing of Saccharomyces cerevisiae we were able to identify several new purine salvage genes such as adenosine kinase or purine nucleoside phosphorylase [ 4 , 5 ] which are highly homologous to their mammalian counterpart. (biomedcentral.com)
• A nucleotidase is a hydrolytic enzyme that catalyzes the hydrolysis of a nucleotide into a nucleoside and a phosphate . (thefullwiki.org)

Enzymes7

• 5'-Nucleotidases (5'NTs) are catabolic enzymes of the nucleotide metabolism. (openthesis.org)
• These results represent the first confirmation of a tick apyrase that belongs to the 5'-nucleotidase family of enzymes. (up.ac.za)
• Lipid peroxidation is followed by loss of structural integrity of plasma membranes thereby releasing the membrane associated enzymes like Adenosine deaminase and 5'-nucleotidase into the circulation. (thefreedictionary.com)
• Electric ray 5′‐nucleotidase shares 61% amino acid identity with the enzymes from rat liver and human placenta, and about 23% with bacterial proteins possessing 5′‐nucleotidase activity and also additional enzyme activities like UDP‐glucose hydrolase. (elsevier.com)
• Interestingly, there are 5-7 domains highly conserved in procaryotes and vertebrates in enzymes exhibiting 5′‐nucleotidase, 3′‐nucleotidase or phosphodiesterase activity. (elsevier.com)
• Enzymes adenosine deaminase (ADA) and 5-nucleotidase (5-'NT) are known to play active role in tissue / cell proliferation and differentiation. (bvsalud.org)
• Soluble 5' nucleotidases are all known to belong to the haloacid dehalogenase superfamily of enzymes which are two domian proteins characterised by a modified Rossman fold as the core and variable cap or hood. (thefullwiki.org)

Antibody5

• This AD2 monoclonal CD73 antibody reacts with human CD73, a 5'-ectonucleotidase that converts 5'-adenosine monophosphate to adenosine. (novusbio.com)
• There are currently no images for 5'-Nucleotidase/CD73 Antibody (NBP2-25235H). (novusbio.com)
• Immunocytochemistry of frozen thin sections with either single or double layers of antibody and visualized with protein A complexed with 5 nm colloidal gold was used to estimate the same density. (gla.ac.uk)
• Nucleotidase/CD73 Monoclonal antibody specifically detects 5 ft. (fishersci.com)
• In order to evaluate the effect of the surface modification on 5'-nucleotidase (5eNT) separation, magnetite with no modification (M), silica-magnetite (MS), silica-aminosilane-magnetite (MSA), silica-amino silane-ScFv antibody-magnetite (MSACF), and silica-aminosilane-anti-CD73 antibody (MSA-CD73) were tested. (thefreedictionary.com)

Abstract1

• Orthologues of Streptococcus pyogenes nuclease A (SpnA) and Streptococcal 5′-nucleotidase A... Soh, Kar Yan;Loh, Jacelyn Mei San;Proft, Thomas 2018-04-05 00:00:00 Abstract Streptococcus pyogenes nuclease A (SpnA) and streptococcal 5′ nucleosidase A (S5nA) are two recently described virulence factors from the human pathogen S. pyogenes. (deepdyve.com)

Phosphate8

• The enzyme has a wide substrate specificity for nucleotides and has been shown to hydrolyze 5'nucleotides rapidly, ribose-5-phosphate slowly, and other phosphate esters extremely slowly (if at all). (wikipedia.org)
• [ 1 ] It facilitates the hydrolysis of the phosphate group from 5'-nucleotides, resulting in corresponding nucleosides. (medscape.com)
• In enzymology, a 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) is an enzyme that catalyzes the chemical reaction adenosine 3',5'-bisphosphate + H2O ⇌ {\displaystyle \rightleftharpoons } adenosine 5'-phosphate + phosphate Thus, the two substrates of this enzyme are adenosine 3',5'-bisphosphate and H2O, whereas its two products are adenosine 5'-phosphate and phosphate. (wikipedia.org)
• A 5'-ribonucleotide + H 2 O = a ribonucleoside + phosphate. (uniprot.org)
• Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. (hmdb.ca)
• This enzyme catalyses the following chemical reaction:a 5'-ribonucleotide + H2O↔ a ribonucleoside + phosphate. (creative-enzymes.com)
• Yeast3',5'-bisphosphate nucleotidase (YND), also known as HAL2, can hydrolyze3'-phosphate from PAP to form AMP or from PAPS to form APS, had been found involved in sulfur metabolism and salt stress. (globethesis.com)
• 5' nucleotidases cleave off the phosphate from the 5' end of the sugar moiety. (thefullwiki.org)

Protein7

• 5'-nucleotidase (5'-NT) is a protein produced by the liver. (medlineplus.gov)
• Cloning of a mouse cytosolic 5'-nucleotidase-I identifies a new gene related to human autoimmune infertility-related protein. (nih.gov)
• The high affinity of ND to PAP and commercial availability for PAP agarose (the cross-linking product of activated agarose and the free amino group from N6-[(6-amino)octyl]-amino-adenosine-3',5'-bisphosphate, Sigma-aldrich,#A3640) enabled affinity purification of YND which suggested YND could be used as a possible fusion affinity tag for protein purification.Affinity chromatography is one of the most popular methods for protein purification. (globethesis.com)
• NT5C (5', 3'-Nucleotidase, Cytosolic) is a Protein Coding gene. (genecards.org)
• LPS upregulated the protein expression but not the messenger RNA expression of E-5′Nu. (hku.hk)
• Therefore, the present study reports that the activity and protein abundance of membrane-bound eN fluctuates across the estrus cycle in the hippocampal synaptosomes of female rats. (ac.rs)
• Selective ERa receptor agonist, PPT, induced upregulation of both the protein level and activity of eN, while application of selective ERb receptor agonist, DPN, increased only the activity of eN. (ac.rs)

Hydrolysis2

• The enzyme catalyzes the hydrolysis of a 5'-ribonucleotide to a ribonucleoside and orthophosphate in the presence of water. (uchicago.edu)
• 5′‐nucleotidase isolated from Torpedo electric organ hydrolyzes UDP‐glucose at 8% of the rate of AMP hydrolysis. (elsevier.com)

Inhibitors2

• Adenosine 5′-[alpha, beta-methylene]diphosphate, ADP and ATP are competitive inhibitors in this order of potency. (portlandpress.com)
• Download the Allosteric inhibitors targeting 5'-nucleotidases to restore poster. (chemaxon.com)

Nucleotide1

• The possible phylogenetic origin of vertebrate 5′‐nucleotidase from multifunctional nucleotide hydrolases is discussed. (elsevier.com)

Purine4

• This gene encodes a hydrolase that serves as an important role in cellular purine metabolism by acting primarily on inosine 5'-monophosphate and other purine nucleotides. (nih.gov)
• Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides. (nih.gov)
• The purine salvage enzyme inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase catalyzes degradation of IMP to inosine. (biomedcentral.com)
• 5' nucleotidases are involved in varied functions like cell-cell communication, nucleic acid repair, purine salvage pathway for the synthesis of nucleotides, signal transduction, membrane transport etc. (thefullwiki.org)

NT5C23

• Cytosolic 5′-nucleotidase II (NT5C2) is involved in the development of 1-β- d -arabinofuranosylcytosine (ara-C) resistance and has been associated with clinical outcome in patients receiving ara-C-based chemotherapy. (aspetjournals.org)
• Of most interest, SNPs (linkage disequilibrium group CEU.12) in the 5′-untranslated region were associated with NT5C2 expression and ara-C sensitivity in HapMap cell lines and with NT5C2 mRNA expression and ara-C sensitivity in diagnostic leukemic blasts from pediatric patients with acute myeloid leukemia. (aspetjournals.org)
• Previous genetic studies demonstrated that chemoresistant ALL is driven by activating mutations in NT5C2 , the gene encoding cytosolic 5´-nucleotidase (cN-II). (biomedcentral.com)

Mammalian4

• 5′-Nucleotidase (5NT) is an intrinsic membrane glycoprotein that is present as an enzyme in a wide variety of mammalian cells. (medscape.com)
• Spiegelberg BD, Xiong JP, Smith JJ, Gu RF, York JD: Cloning and characterization of a mammalian lithium-sensitive bisphosphate 3'-nucleotidase inhibited by inositol 1,4-bisphosphate. (hmdb.ca)
• 5'-nucleotidase is an intrinsic membrane glycoprotein, present as an ectoenzyme in a wide variety of mammalian cells and it hydrolyzes extracellular AMP to adenosine and represents the major control point for extracellular adenosine levels and is a regulator of the adenosine signaling pathway. (thefreedictionary.com)
• Polyclonal antibodies raised against 5′‐nucleotidase from mammalian sources or the electric ray electric organ reveal mutual crossreactivity. (elsevier.com)

Hydrolase1

• The crystal structure of 5'-nucleotidase (5'-NT) from E. coli, also known as UDP-sugar hydrolase, has been determined at 1.7 A resolution. (proteopedia.org)

Phosphatase2

• Other names in common use include phosphoadenylate 3'-nucleotidase, 3'-phosphoadenylylsulfate 3'-phosphatase, phosphoadenylate 3'-nucleotidase, and 3'(2'),5'-bisphosphonucleoside 3'(2')-phosphohydrolase. (wikipedia.org)
• Gene Ontology (GO) annotations related to this gene include phosphatase activity and nucleotidase activity . (genecards.org)

Catalyzes1

• 5'-nucleotidase (EC 3.1.3.5) is an enzyme which catalyzes the phosphorylytic cleavage of 5'nucleotides. (wikipedia.org)

Soluble3

• One of the soluble forms appears to be derived from the GPI-anchored ex-5'-nucleotidase and has an extracellular location. (wikipedia.org)
• Vascular leakage secondary to hypoxia was reversed in part by adenosine receptor agonists or reconstitution with soluble 5′-nucleotidase. (rupress.org)
• The isoforms were predicted to be soluble secreted proteins, which correlated with the trend observed for parasitic apyrases in the 5'-nucleotidase family. (up.ac.za)

Monophosphates2

• Nucleotidase with a broad substrate specificity as it can dephosphorylate various ribo- and deoxyribonucleoside 5'-monophosphates and ribonucleoside 3'-monophosphates with highest affinity to 3'-AMP. (rcsb.org)
• Membrane bound 5' nucleotidases displays specificity towards adenosine monophosphates and are predominantly involved in the salvage of preformed nucleotides and in signal transduction cascades involving purinergic receptors. (thefullwiki.org)

Intracellular1

• Further, we analyzed the role of E2 and its intracellular receptors on the expression of eN in ovariectomized females. (ac.rs)

Metabolism1

• Previous findings obtained on adenosine metabolism in brain suggest that eN may be modulated by ovarian steroids. (ac.rs)

Genes1

• Strikingly, no 5'-nucleotidase encoding gene could be inferred from comparison of the completely sequenced yeast genome with 5'-nucleotidase genes from other species, although several lines of evidence argued for the presence of 5'-nucleotidase in yeast. (biomedcentral.com)

Activity14

• This is due to the fact that the enzyme activity is approximately 10-times higher for peripheral T cells than thymocytes, 5-6 times higher in adult peripheral B cells than fetal B cells, and largely absent in non-T cell and non-B cell lymphocytes. (wikipedia.org)
• A cDNA coding for bovine cytosolic IMP/GMP-specific 5ʹ-nucleotidase endowed with phosphotransferase activity was cloned from calf thymus RNA, by 5ʹ and 3ʹ rapid amplification of cDNA ends protocols (5ʹ and 3ʹ RACE). (biochemj.org)
• It exhibited both 5ʹ-nucleotidase and phosphotransferase activity, as well as immunological and kinetic properties similar to those of the enzyme purified from calf thymus. (biochemj.org)
• Escherichia coli 5′-nucleotidase activity is stimulated 30- to 50-fold in vitro by the addition of Co 2+ . (biochemj.org)
• The studies reported herein suggest that displacement occurs at the metal-ion-binding site consisting of residues Asp 84 , Asn 116 , His 217 and His 252 , leading to the observed increase in 5′-nucleotidase activity. (biochemj.org)
• Dephosphorylates the 5 and 2(3)-phosphates of deoxyribonucleotides, with a preference for dUMP and dTMP, intermediate activity towards dGMP, and low activity towards dCMP and dAMP. (genecards.org)
• E-5′Nu activity was enhanced after a 24 h exposure to LPS. (hku.hk)
• At 10 μM, the phosphatidylinositol 3-kinase (PI3K) inhibitor LY-294002 abolished the LPS-induced E-5′Nu activity. (hku.hk)
• In conclusion, the experiments indicate that LPS upregulates E-5′Nu activity in HUVECs through a PI3K-dependent increase in the abundance of E-5′Nu on cell membranes. (hku.hk)
• Although this enzymatic activity has been purified and characterized in Saccharomyces cerevisiae , the gene encoding IMP 5'-nucleotidase had not been identified. (biomedcentral.com)
• Knock-out of ISN1 resulted in the total loss of IMP-specific 5'-nucleotidase activity, thus confirming that the ISN1 gene indeed encodes the enzymatic activity purified from yeast. (biomedcentral.com)
• We have identified a new yeast gene, ISN1 ( YOR155c ), as encoding IMP-specific 5'-nucleotidase activity. (biomedcentral.com)
• The 5'-NT activity, however, did not show any marked difference between the two horns under Gr.I and II in both species. (bvsalud.org)
• and (3) the activity of enzyme 5'-NT does not alter during the deciduoma formation in both the species. (bvsalud.org)

CDNA3

• The similarity in the coding region to that of the human 5ʹ-nucleotidase cDNA sequence [Oka, Matsumoto, Hosokawa and Inoue (1994) Biochem. (biochemj.org)
• The bovine cDNA sequence included the sequences codifying for six peptides obtained from 5ʹ-nucleotidase/phosphotransferase purified from calf thymus. (biochemj.org)
• A cDNA encoding a 5′‐nucleotidase was identified by screening a λgt10 cDNA library from the electric lobe of Discopyge ommata using a cDNA probe containing the complete open reading frame coding for the rat liver enzyme. (elsevier.com)

Allosteric2

• Inactive variants of the mitochondrial and cytosolic deoxynucleotidases and the cytosolic 5'-nucleotidase II were used to characterize the structural basis for their substrate specificity in high detail.Based on structures of the apoprotein and activator/activator+substrate complexes of cytosolic 5'-nucleotidase II, a mechanism for the allosteric activation of this enzyme was presented. (openthesis.org)
• Unlike other nucleotidases, it is subject to complex allosteric regulation. (biomedcentral.com)

Monophosphate2

• 5'-NT creates adenosine from adenosine monophosphate (AMP). (dovemed.com)
• One unit will hydrolyze 1.0 μmole of inorganic phosphorus from adenosine 5'-monophosphate per min at pH 9.0 at 37°C. (creative-enzymes.com)

Phosphohydrolase2

• The systematic name of this enzyme class is adenosine-3'(2'),5'-bisphosphate 3'(2')-phosphohydrolase. (wikipedia.org)
• 5'-nucleotidase is an enzyme with system name 5'-ribonucleotide phosphohydrolase. (creative-enzymes.com)

Lymphocytes3

• Citations to Human lymphocytes: 5'-nucleotidase-positive and -negative subpopulations. (jci.org)
• Human lymphocytes: 5'-nucleotidase-positive and -negative subpopulations. (jci.org)
• The enzyme, 5'-nucleotidase (5'N) (E.C.-3.1.3.5) is present in lymphocytes isolated from the blood of normal subjects. (jci.org)

Escherichia1

• X-ray structure of the Escherichia coli periplasmic 5'-nucleotidase containing a dimetal catalytic site. (proteopedia.org)

Inhibition1

• The inhibition of E-5′Nu by 100 μM α,β-methylene adenosine-5′-diphosphate increased the LPS-induced inflammation, suggesting that E-5′Nu plays a significant role in reducing inflammation, probably through the generation of adenosine. (hku.hk)

Specificity1

• This enzyme has a wide specificity for 5'-nucleotides. (creative-enzymes.com)

Human4

• Primary structure of human placental 5′-nucleotidase and identification of the glycolipid anchor in the mature form. (dovemed.com)
• Nucleotidase/CD73 in Human samples. (fishersci.com)
• In the present study, the effect of lipopolysaccharide (LPS), an inflammation inducer, was investigated on E-5′Nu in human umbilical vein endothelial cells (HUVECs). (hku.hk)
• nucleotidase in human glioblastoma. (edu.pl)

Endothelial1

• Since adenosine is an anti-inflammatory molecule, E-5′Nu upregulation may be crucial in protecting endothelial cells against inflammatory damage. (hku.hk)

NTPDase1

• Piracetam prevents scopolamine-induced memory impairment and decrease of NTPDase, 5'-nucleotidase and adenosine deaminase activities," Neurochemical Research, vol. (thefreedictionary.com)

Liver5

• 5'-Nucleotidase (5'-NT) is an enzyme found in the liver. (dovemed.com)
• 5'-NT appears in blood when the liver is experiencing problems. (dovemed.com)
• The main cause for this rise in 5'-NT levels is blockage of the passageways connecting the liver, duodenum, and gallbladder. (dovemed.com)
• This test measures the levels of 5'- nucleotidase (an enzyme specific to the liver). (rochester.edu)
• The 5'- nucleotidase level is elevated in persons with liver diseases, especially those diseases associated with cholestasis. (rochester.edu)

Coli1

• Seven residues from conserved sequence motifs implicated in the catalytic and metal-ion-binding sites of E. coli 5′-nucleotidase (Asp 41 , His 43 , Asp 84 , His 117 , Glu 118 , His 217 and His 252 ) were selected for modification using site-directed mutagenesis of the cloned ushA gene. (biochemj.org)

Membrane2

• The 5'-nucleotidase of guinea pig polymorphonuclear leukocytes is localized exclusively on the plasma membrane of intact cells. (sciencemag.org)
• Several independent methods were used to determine the density of 5'-nucleotidase on the plasma membrane of the Fao cell. (gla.ac.uk)

Enzymatic1

• Non-enzymatic functions of eN have also been reported. (edu.pl)

Molecule2

• The function of 5'-NT is to create the molecule adenosine. (dovemed.com)
• CD39 can also convert the latter molecule into AMP, fully dephosphorylated to ADO by the 5'-nucleotidase (5'-NT) CD73 [7]. (thefreedictionary.com)

Serum1

• Serum levels of 5′-nucleotidase in disease. (medscape.com)