A class of enzymes that catalyze the conversion of a nucleotide and water to a nucleoside and orthophosphate. EC 3.1.3.-.
A glycoprotein enzyme present in various organs and in many cells. The enzyme catalyzes the hydrolysis of a 5'-ribonucleotide to a ribonucleoside and orthophosphate in the presence of water. It is cation-dependent and exists in a membrane-bound and soluble form. EC
A calcium-activated enzyme that catalyzes the hydrolysis of ATP to yield AMP and orthophosphate. It can also act on ADP and other nucleoside triphosphates and diphosphates. EC
Cytidine (dihydrogen phosphate). A cytosine nucleotide containing one phosphate group esterified to the sugar moiety in the 2', 3' or 5' position.
A species of halophilic archaea found in the Dead Sea.
Pyrimidines with a RIBOSE and phosphate attached that can polymerize to form DNA and RNA.
A disease of cats and mink characterized by a marked inflammation of adipose tissue and the deposition of "ceroid" pigment in the interstices of the adipose cells. It is believed to be caused by feeding diets containing too much unsaturated fatty acid and too little vitamin E. (Merck Veterinary Manual, 5th ed; Stedman, 25th ed)
A condition of inadequate circulating red blood cells (ANEMIA) or insufficient HEMOGLOBIN due to premature destruction of red blood cells (ERYTHROCYTES).
5'-Uridylic acid. A uracil nucleotide containing one phosphate group esterified to the sugar moiety in the 2', 3' or 5' position.
Adenine nucleotide containing one phosphate group esterified to the sugar moiety in the 2'-, 3'-, or 5'-position.
A group of hydrolases which catalyze the hydrolysis of monophosphoric esters with the production of one mole of orthophosphate. EC 3.1.3.
A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.
A group of enzymes within the class EC 3.6.1.- that catalyze the hydrolysis of diphosphate bonds, chiefly in nucleoside di- and triphosphates. They may liberate either a mono- or diphosphate. EC 3.6.1.-.
A nucleoside that is composed of ADENINE and D-RIBOSE. Adenosine or adenosine derivatives play many important biological roles in addition to being components of DNA and RNA. Adenosine itself is a neurotransmitter.
The rate dynamics in chemical or physical systems.
Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.
Proteins found in any species of archaeon.
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
The monomeric units from which DNA or RNA polymers are constructed. They consist of a purine or pyrimidine base, a pentose sugar, and a phosphate group. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
A large multisubunit complex that plays an important role in the degradation of most of the cytosolic and nuclear proteins in eukaryotic cells. It contains a 700-kDa catalytic sub-complex and two 700-kDa regulatory sub-complexes. The complex digests ubiquitinated proteins and protein activated via ornithine decarboxylase antizyme.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The sum of the weight of all the atoms in a molecule.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
Animals having a vertebral column, members of the phylum Chordata, subphylum Craniata comprising mammals, birds, reptiles, amphibians, and fishes.
A phosphoric diester hydrolase that removes 5'-nucleotides from the 3'-hydroxy termini of 3'-hydroxy-terminated OLIGONUCLEOTIDES. It has low activity towards POLYNUCLEOTIDES and the presence of 3'-phosphate terminus on the substrate may inhibit hydrolysis.
Solutions or mixtures of toxic and nontoxic substances elaborated by snake (Ophidia) salivary glands for the purpose of killing prey or disabling predators and delivered by grooved or hollow fangs. They usually contain enzymes, toxins, and other factors.
Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
The portion of an interactive computer program that issues messages to and receives commands from a user.
Sequential operating programs and data which instruct the functioning of a digital computer.
A subtype of INFLUENZA A VIRUS comprised of the surface proteins hemagglutinin 2 and neuraminidase 2. The H2N2 subtype was responsible for the Asian flu pandemic of 1957.
The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent.
A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.
Compounds containing carbohydrate or glycosyl groups linked to phosphatidylinositols. They anchor GPI-LINKED PROTEINS or polysaccharides to cell membranes.
Antibodies produced by a single clone of cells.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
The dynamic collection of metabolites which represent a cell's or organism's net metabolic response to current conditions.
The systematic identification and quantitation of all the metabolic products of a cell, tissue, organ, or organism under varying conditions. The METABOLOME of a cell or organism is a dynamic collection of metabolites which represent its net response to current conditions.
A subclass of purinergic P2Y receptors that have a preference for ATP and ADP. The activated P2Y1 receptor signals through the G-PROTEIN-coupled activation of PHOSPHOLIPASE C and mobilization of intracellular CALCIUM.
Solitary or multiple benign cutaneous nodules comprised of immature and mature vascular structures intermingled with endothelial cells and a varied infiltrate of eosinophils, histiocytes, lymphocytes, and mast cells.
... nucleotidase (cN1A; NT5C1A) have been strongly associated with the condition. In the clinical context of a classic history and ... 27 (5): 591-598. doi:10.1097/WCO.0000000000000129. PMC 4248565. PMID 25159931. Machado, P; Brady, S; Hanna, MG (2013). "Update ... Patients may become unable to perform activities of daily living and most require assistive devices within 5 to 10 years of ... 35 (5): 549-561. doi:10.1002/mus.20766. PMID 17366591. S2CID 23665612. "Sporadic Inclusion Body Myositis". NORD (National ...
... nucleotidase, cytosolic IA is a protein that in humans is encoded by the NT5C1A gene. Cytosolic nucleotidases, such as NT5C1A, ... nucleotidase I: characterization and role in nucleoside analog resistance". J. Biol. Chem. 276 (13): 10498-504. doi:10.1074/jbc ... nucleotidase, cytosolic IA". Hunsucker SA, Spychala J, Mitchell BS (2001). "Human cytosolic 5'- ...
... nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. CD73 commonly ... nucleotidase, ecto (CD73)". St Hilaire C, Ziegler SG, Markello TC, Brusco A, Groden C, Gill F, et al. (February 2011). "NT5E ... nucleotidase) at the PDBe-KB. This article incorporates text from the United States National Library of Medicine, which is in ... nucleotidase". Life Sciences. 62 (25): 2257-66. doi:10.1016/S0024-3205(98)00206-9. PMID 9651114. NT5E+protein,+human at the US ...
Of the different forms of nucleotidases characterized, this enzyme is unique in its preference for 5'-dNTP. It may be one of ... This gene encodes a nucleotidase that catalyzes the dephosphorylation of the 5' deoxyribonucleotides (dNTP) and 2'(3')-dNTP and ... nucleotidase mRNA levels in blast cells are a prognostic factor in acute myeloid leukemia patients treated with cytarabine". ... nucleotidase mRNA levels in blast cells are a prognostic factor in acute myeloid leukemia patients treated with cytarabine". ...
... nucleotidase as well as of a number of the nucleoside triphosphate and diphosphate-hydrolyzing enzymes of the family of the ... nucleotidase from the electric ray electric lobe. Primary structure and relation to mammalian and procaryotic enzymes". Biochem ... nucleotidase from Torpedo electric organ". Biochem. J. 245 (3): 805-810. doi:10.1042/bj2450805. PMC 1148201. PMID 2822031. ... "Cellular function and molecular structure of ecto-nucleotidases". Purinergic Signal. 8 (3): 437-502. doi:10.1007/s11302-012- ...
... nucleotidase, and phosphodiesterase. The skin of the blue-spotted stingray is often used for drums, such as on the Arab and ...
1995). "Homogeneous pyrimidine nucleotidase from human erythrocytes: enzymic and molecular properties". Biochem. J. 304 (Pt 3 ... 1998). "Pyrimidine nucleotidases from human erythrocyte possess phosphotransferase activities specific for pyrimidine ... nucleotidase (PN-I or P5'NI), and p56, is an enzyme that in humans is encoded by the NT5C3, or NT5C3A, gene on chromosome 7. ... nucleotidase family and one of the five cytosolic members identified in humans. NTC53 catalyzes the dephosphorylation of the ...
... nucleotidase. The nucleoside, adenosine, is then deaminated and hydrolyzed to form hypoxanthine via adenosine deaminase and ... Nelson, David L.; Cox, Michael M.; Lehninger, Albert L. (2008). Lehninger's Principles of Biochemistry (5 ed.). Macmillan. ISBN ... First, the nucleotide will lose its phosphate through 5'- ...
... nucleotidase (CD73) and A2B adenosine receptors". Circulation. 115 (12): 1581-90. doi:10.1161/CIRCULATIONAHA.106.669697. PMID ... 5 (6): e137. doi:10.1371/journal.pmed.0050137. PMID 18578565. Eckle T, Grenz A, Laucher S, Eltzschig HK (October 2008). "A2B ...
... nucleotidase family". Exp. Parasitol. 122 (4): 318-27. doi:10.1016/j.exppara.2009.04.007. PMID 19393241. http://www.moh.gov.sa/ ... 13 (1): 153-5. doi:10.3201/eid1301.061094. PMC 2725816. PMID 17370534. Rico-Hesse, Rebeca; Dodd, Kimberly A.; Bird, Brian H.; ... 15 (5-6): 570-571. doi:10.1093/jmedent/15.5-6.570. Schlein, Y.; Gunders, A. E. (2009). "Pheromone of Ornithodoros spp. ( ... 19 (5): 325-332. doi:10.1002/psc.2505. hdl:2263/39717. PMID 23553969. "Ornithodoros spp - Integumentary System". "AgriEco , ...
... nucleotidase. Adenosine constricts the afferent arteriole by binding with high affinity to the A1 receptors a Gi/Go. Adenosine ... nucleotidase (CD73) (Le Hir and Kaissling, 1993). This enzyme catalyzes the dephosphorylation of 5′-AMP or 5′-IMP to adenosine ... nucleotidase (Thomson et al., 2000). Extracellular adenosine activates A1 receptors on vascular afferent arteriolar smooth ... 299 (2): F310-5. doi:10.1152/ajprenal.00149.2010. PMC 2928524. PMID 20462966. Ortiz-Capisano MC, Atchison DK, Harding P, Lasley ...
56 (5): 326-329. Archived from the original (PDF) on 2011-07-25. Retrieved 2009-04-28. Ninfa AJ, Ballou DP, Benore M (2010). ... 27 (5): 1489-95. doi:10.1016/j.tiv.2013.03.002. PMID 23524101. Chou JY, Robinson JC (July 1977). "Induction of placental ... 5 (4): 817-30. doi:10.1016/s0950-3552(05)80289-5. PMID 1822819. Retrieved 1 May 2016. Lange PH, Millan JL, Stigbrand T, ... doi:10.1186/s40697-015-0066-5. PMC 4538753. PMID 26284153. Mizumori M, Ham M, Guth PH, Engel E, Kaunitz JD, Akiba Y (July 2009 ...
... nucleotidase 2 (dNT-2), is an enzyme that in humans is encoded by the NT5M gene. This gene encodes a 5' nucleotidase that ... nucleotidases identified in humans, all of which differ in tissue specificity, subcellular location, primary structure and ... nucleotidase, mitochondrial". CS1 maint: discouraged parameter (link) Rampazzo C, Gallinaro L, Milanesi E, Frigimelica E, ... nucleotidase, mitochondrial, also known as 5'(3')-deoxyribonucleotidase, mitochondrial (mdN) or deoxy-5'- ...
... nucleotidase deficiency--congenital hemolytic anemia with basophilic stippling of erythrocytes". Tidsskrift for den Norske ... nucleotidase deficiency Alcoholism Myelodysplastic syndromes Sideroblastic anemia Congenital dyserythropoietic anemia Primary ... 5 (4): 327-34. doi:10.1002/ajim.4700050409. PMID 6202140. Tkachuk, Douglas C.; Hirschmann, Jan V.; Wintrobe, Maxwell Myer (2007 ... Severe megaloblastic anemia Hemolytic anemia Sickle-cell anemia Pyrimidine 5' ...
... nucleotidase, important for the correct metabolism of DNA and can therefore cause fetal damage. "Lead compounds (as Pb)". ... nucleotidase activity in workers exposed to lead, mercury or cadmium". Int Arch Occup Environ Health. 55 (3): 247-52. doi: ... At higher pH Pb6(OH)5(NO3) is formed. The cation [Pb6O(OH)6]4+ is unusual in having an oxide ion inside a cluster of 3 face- ... 5 (3): 242. doi:10.1021/je60007a002. Pauley, J. L.; M. K. Testerman (1954). "Basic Salts of Lead Nitrate Formed in Aqueous ...
A nucleotidase is an enzyme that catalyzes the hydrolysis of a nucleotide, forming a nucleoside and a phosphate ion. ... Inside the cell, nucleotidases may help to maintain energy levels under stress conditions. A cell deprived of oxygen and ... Some nucleotidases function outside the cell, creating nucleosides that can be transported into the cell and used to regenerate ... Nucleotidases are essential for cellular homeostasis, because they are partially responsible for maintaining a balanced ratio ...
... nucleotidase activity via Ca2+-dependent, annexin 2-mediated membrane rearrangement?". Biochemical Society Transactions. 34 (Pt ... 33 (5): 1223-8. doi:10.1021/bi00171a023. PMID 8110754. Wright JF, Kurosky A, Wasi S (Feb 1994). "An endothelial cell-surface ... Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". The Journal of Biological Chemistry. 277 ( ... doi:10.1016/S0300-9084(02)00027-5. PMID 12628297. Kirshner J, Schumann D, Shively JE (Dec 2003). "CEACAM1, a cell-cell adhesion ...
... nucleotidase) in 165 glioblastomas by immunohistochemistry and electronmicroscopic histochemistry". Anticancer Res. 19 (3A): ... 272 (5): 2814-20. doi:10.1074/jbc.272.5.2814. ISSN 0021-9258. PMID 9006922. S2CID 24800195. Talts, J F; Sasaki T; Miosge N; ... 5 (4): 932-5. doi:10.1016/0888-7543(89)90136-5. PMID 2591971. "Entrez Gene: LAMA1 laminin, alpha 1". Utani, A; Nomizu M; Yamada ... 272 (5): 2814-20. doi:10.1074/jbc.272.5.2814. PMID 9006922. S2CID 24800195. Cáceres J, Brandan E (1997). "Interaction between ...
Zimmermann H, Zebisch M, Sträter N (September 2012). "Cellular function and molecular structure of ecto-nucleotidases". ... nucleotidase (eN) as well as by APs. In any case, the final product of the hydrolysis cascade is the nucleoside. The Pannexin-1 ... nucleotidase (CD73) (Le Hir and Kaissling, 1993). This enzyme catalyzes the dephosphorylation of 5′-AMP or 5′-IMP to adenosine ... nucleotidase (Thomson et al., 2000). Extracellular adenosine activates A1 receptors on vascular afferent arteriolar smooth ...
... nucleotidase which has been extensively characterized kinetically. The tetrameric enzyme shows aspects of positive homotropic ... "Allosteric regulation and substrate activation in cytosolic nucleotidase II from Legionella pneumophila". FEBS Journal. 281 (6 ... 193 (5): 1114-21. doi:10.1128/JB.01146-10. PMC 3067580. PMID 21169481. Cianciotto, Nicholas P (May 2015). "An update on iron ... 9 (5): 1313. doi:10.1128/mBio.01313-18. PMC 6178616. PMID 30301851. Michod RE, Bernstein H, Nedelcu AM (May 2008). "Adaptive ...
Srinivasan B, Balaram H (2007). "ISN1 nucleotidases and HAD superfamily protein fold: in silico sequence and structure analysis ... nucleotidases catalyze the hydrolysis of purine mononucleotides to their respective nucleosides and phosphate. The nucleosides ... "Allosteric regulation and substrate activation in cytosolic nucleotidase II from Legionella pneumophila". The FEBS Journal. 281 ... Phosphoribosyltransferases add activated ribose-5-phosphate (Phosphoribosyl pyrophosphate, PRPP) to bases, creating nucleoside ...
... nucleotidase and alkaline phosphatase (ALP) enzymes. With a few exceptions, the optimal test for cholestasis would be ... Cholestasis can be suspected when there is an elevation of both 5'- ...
... nucleotidase (5NCT) family, is also capable of dephosphorylating cladribine, making it inactive. The most important subtype of ... 173 (5): 663-70. doi:10.1111/bjh.13955. PMID 26913480. The combination of cytarabine and cladribine is the current standard for ... 5 (1): 1-8. doi:10.3109/10428199109068099. PMID 27463204. Baker, D; Marta, M; Pryce, G; Giovannoni, G; Schmierer, K (February ... 362 (5): 416-26. doi:10.1056/NEJMoa0902533. PMID 20089960. Johnston, JB (June 2011). "Mechanism of action of pentostatin and ...
... nucleotidase deficiency, and intraerythrocytic accumulation of pyrimidines". Journal of Clinical Investigation. 58 (4): 926-932 ... Hemolytic anemia accounts for 5% of all existing anemias. It has numerous possible consequences, ranging from general symptoms ... 5), 1232-1237. https://doi.org/10.2215/CJN.01960408 Current Medical Diagnosis and Treatment 2009 By Stephen J. McPhee, Maxine A ...
... nucleotidase and decrease in guanylate kinase activity in the brain and liver of irradiated rats]". Ukr. Biokhim. Zh. 62 (2): ... Boritzki TJ, Jackson RC, Morris HP, Weber G (1981). "Guanosine-5'-phosphate synthetase and guanosine-5'-phosphate kinase in rat ... doi:10.1016/S0076-6879(78)51066-5. ISBN 978-0-12-181951-4. PMID 211390. Overview of all the structural information available in ...
... nucleotidase I activity in MOLT-4 cells". Nucleosides Nucleotides Nucleic Acids. 25 (9-11): 1201-4. doi:10.1080/ ... 62 (5): 745-7. doi:10.1001/archneur.62.5.745. PMID 15883261. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N ... 11 (5): 677-84. doi:10.1101/gr.gr-1640r. PMC 311086. PMID 11337467. Johansson K, Ramaswamy S, Ljungcrantz C, Knecht W, Piskur J ... 293 (5): 1489-96. doi:10.1016/S0006-291X(02)00415-1. PMID 12054684. Salviati L, Sacconi S, Mancuso M, Otaegui D, Camaño P, ...
... nucleotidases (EC), 3'-nucleotidases (EC) and exopolyphosphatases (EC). The gene, surE, is part of a bicistronic operon found ... 326 (5): 1559-75. doi:10.1016/S0022-2836(03)00056-1. PMID 12595266. This article incorporates text from the public domain Pfam ... This domain is found in acid phosphatases (EC), 5'- ...
... nucleotidase". Biochimica et Biophysica Acta. 33 (1): 101-5. doi:10.1016/0006-3002(59)90502-5. PMID 13651188. Biology portal v ... In enzymology, a 5'-acylphosphoadenosine hydrolase (EC is an enzyme that catalyzes the chemical reaction 5'- ... This enzyme is also called 5-phosphoadenosine hydrolase. This enzyme participates in purine metabolism. Kellerman GM (May 1959 ... The systematic name of this enzyme class is 5'-acylphosphoadenosine acylhydrolase. ...
... nucleotidase, thymidylate nucleotidase, deoxythymidylic 5'-nucleotidase, deoxythymidylate phosphohydrolase, and dTMPase. ... nucleotidase. Purification and properties of an enzyme found after infection of Bacillus subtilis with phage SP5C". J. Biol. ... In enzymology, a thymidylate 5'-phosphatase (EC is an enzyme that catalyzes the chemical reaction thymidylate + H2O ... The systematic name of this enzyme class is thymidylate 5'-phosphohydrolase. Other names in common use include thymidylate 5'- ...
... nucleotidases. Although the bacteria are conventionally divided into two main groups-gram-positive and gram-negative, based ...
... -Activating Nucleotidase).[26] The association of the 19S and 20S particles requires the binding of ATP to the 19S ... 66 (5): 309-317. doi:10.1002/iub.1271. PMID 24823973.. *^ a b Nobel Prize Committee (2004). "Nobel Prize Awardees in Chemistry ... 109 (5): 671-9. doi:10.1172/JCI12736. PMC 150886. PMID 11877475.. *^ Elliott PJ, Pien CS, McCormack TA, Chapman ID, Adams J ( ... 5 (22): 2592-601. doi:10.4161/cc.5.22.3448. PMID 17106261.. *^ Park SH, Bolender N, Eisele F, Kostova Z, Takeuchi J, Coffino P ...
... nucleotidase.[9] Sucrose-tris(hydroxymethyl)aminomethane-HCl medium is essential to the preservation of the integrity of the ...
Nucleotidase of rat liver microsomes". J. Biol. Chem. 235: 471-474. PMID 14444527. ... B enzm: 1.1/2/3/4/5/6/7/8/10/11/13/14/15-18, 2.1/2/3/4/5/6/7/8, 2.7.10, 2.7.11-12, 3.1/2/3/4/5/6/7,, 3.4.21/22/23/24, ... 5'-ribonukleotid + H2O ⇌. {\displaystyle \rightleftharpoons }. ribonukleozid + fosfat. Ovaj enzim ima široku specifičnost za 5 ... 5'-nukleotidaza (EC, uridinska 5'-nukleotidaza, 5'-adenilna fosfataza, adenozinska 5'-fosfataza, AMP fosfataza, ...
53 (5): 1110-7. doi:10.1095/biolreprod53.5.1110. PMID 8527515.. *^ Langin D, Laurell H, Holst LS, Belfrage P, Holm C (June 1993 ... Hormone-sensitive lipase (EC, HSL), also previously known as cholesteryl ester hydrolase (CEH),[5] sometimes referred ... Nucleotidase. *Glucose 6-phosphatase. *Fructose 1,6-bisphosphatase *Calcineurin. *Protein phosphatase *PP2A ...
... nucleotidase II (cN-II), where the affinity for substrate GMP increases upon GTP binding at the dimer interface[10] ... Jan;5(1):365-8. *^ E. K. Jaffe (2005). "Morpheeins - a new structural paradigm for allosteric regulation". Trends Biochem. Sci ... 17 (5): 2895-2918.. *^ Cuendet, M.A., Weinstein, H., and LeVine, M.V. (2016) "The allostery landscape: quantifying ... "Allosteric regulation and substrate activation in cytosolic nucleotidase II from Legionella pneumophila". FEBS J. 281 (6): ...
Nucleotidase. *Glucose 6-phosphatase. *Fructose 1,6-bisphosphatase *Calcineurin. *Protein phosphatase *PP2A ...
Nucleotidase. *Glucose 6-phosphatase. *Fructose 1,6-bisphosphatase *Calcineurin. *Protein phosphatase *PP2A ... In this study it was also shown that PETases can degrade polyethylene 2,5-furandicarboxylate (PEF).[2][10] ... 5] Discoveries of polyester degrading enzymes date at least as far back as 1975 (α-chymotrypsin)[6] and 1977 (lipase) for ... 5-furandicarboxylate), which is a bioderived PET replacement. PETases can't catalyze the hydrolysis of aliphatic polyesters ...
Nucleotidase. *Glucose 6-phosphatase. *Fructose 1,6-bisphosphatase *Calcineurin. *Protein phosphatase *PP2A ... 5, Academic Press, New York, 1961, p. 73-78.. *. Neuhaus FC, Byrne WL (1959). "Metabolism of phosphoserine. II. Purification ...
NAD+ nucleotidase, cyclic ADP-ribose generating. • NAD+ nucleosidase activity. • phosphorus-oxygen lyase activity. • identical ... 113 (5): 492-498. doi:10.1111/vox.12653. PMID 29781081.. *^ Camacho-Pereira J, Tarragó MG, Chini CC, Nin V, Escande C, Warner ... 35 (5): 417-32. doi:10.1615/CritRevImmunol.v35.i5.50. PMID 26853852.. *^ Deaglio S, Mehta K, Malavasi F (January 2001). "Human ... 144 (7): 2811-5. PMID 2319135.. *^ Nata K, Takamura T, Karasawa T, Kumagai T, Hashioka W, Tohgo A, Yonekura H, Takasawa S, ...
2000). "Human monocyte derived dendritic cells express functional P2X and P2Y receptors as well as ecto-nucleotidases.". FEBS ... svi osim 5-HT3) 5-HT1 (A, B, D, E, F) • 5-HT2 (A, B, C) • 5-HT (4, 5A, 6, 7) ... B/W (1, 2) • FF (1, 2) • S • Y (1, 2, 4, 5) • Neuromedin (B, U (1, 2)) • Neurotenzin (1, 2) ... Adrenomedulin • Mirisni • Opsin (3, 4, 5, 1LW, 1MW, 1SW, RGR, RRH) • Proteazom-aktivirani (1, 2, 3, 4) • SREB (1, 2, 3) ...
CANT1: Calcium-activated nucleotidase 1 (17q25.3). *BIRC5: Survivin (17q25.3). *CHMP6: Charged multivesicular body protein 6 ( ... doi:10.1186/gb-2010-11-5-206. PMC 2898077. PMID 20441615.. *^ "Statistics & Downloads for chromosome 17". HUGO Gene ... So CCDS's gene number prediction represents a lower bound on the total number of human protein-coding genes.[5] ... SPAG5: encoding protein Sperm-associated antigen 5. *ST6GALNAC1: encoding enzyme Alpha-N-acetylgalactosaminide alpha-2,6- ...
Nucleotidase - فوسفاتاز قلوي - Tartrate resistant acid phosphatase - Purple acid phosphatases - غلوكوز 6-فسفاتاز - Fructose ... phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity. • phosphatidylinositol-3-phosphatase activity. • protein ... Autotaxin - Phospholipase C - Phospholipase D - cGMP specific phosphodiesterase type 5 - Sphingomyelin phosphodiesterase ... inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity. • PDZ domain binding. ...
... nucleotidase, binding of one GMP molecule to a single subunit of the tetrameric enzyme leads to increased affinity for GMP by ... nucleotidase II (cN-II), where the affinity for substrate GMP increases upon GTP binding at the dimer interface[1] ... nucleotidase, binding of GTP molecule at the dimer interface in the tetrameric enzyme leads to increased affinity for substrate ... 5 (1): 365-85. PMID 5938952.. *^ a b Cuendet MA, Weinstein H, LeVine MV (December 2016). "The Allostery Landscape: Quantifying ...
Arsenis, C. and Touster, O. (1968). "Purification and properties of an acid nucleotidase from rat liver lysosomes". J. Biol. ... B enzm: 1.1/2/3/4/5/6/7/8/10/11/13/14/15-18, 2.1/2/3/4/5/6/7/8, 2.7.10, 2.7.11-12, 3.1/2/3/4/5/6/7,, 3.4.21/22/23/24, ... B enzm: 1.1/2/3/4/5/6/7/8/10/11/13/14/15-18, 2.1/2/3/4/5/6/7/8, 2.7.10, 2.7.11-12, 3.1/2/3/4/5/6/7,, 3.4.21/22/23/24, ... RNaza III • RNaza H (1, 2A, 2B, 2C) • RNaza P • RNaza A (1, 2, 3, 4/5) • RNaza T1 • RNK-indukovani utišavajući kompleks ...
Phospholipase C, gamma 1, also known as PLCG1, is a protein that in humans is encoded by the PLCG1 gene.[5][6] ... Nucleotidase. *Glucose 6-phosphatase. *Fructose 1,6-bisphosphatase *Calcineurin. *Protein phosphatase *PP2A ... The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from ... phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the ...
... is an enzyme that in humans is encoded by the RNASE2 gene.[5][6][7] ... Nucleotidase. *Glucose 6-phosphatase. *Fructose 1,6-bisphosphatase *Calcineurin. *Protein phosphatase *PP2A ... 143 (3): 952-5. PMID 2745977.. *. Beintema JJ, Hofsteenge J, Iwama M, et al. (1988). "Amino acid sequence of the nonsecretory ... 2bzz: CRYSTAL STRUCTURES OF EOSINOPHIL-DERIVED NEUROTOXIN IN COMPLEX WITH THE INHIBITORS 5'-ATP, AP3A, AP4A AND AP5A ...
5: 307-30. PMID 165672.. *^ a b Rivet-Bastide M, Vandecasteele G, Hatem S, et al. (June 1997). "cGMP-stimulated cyclic ... Some are cAMP (figure 1) selective hydrolases (PDE 4, -7 and -8), others are cGMP (figure 1) selective hydrolases (PDE 5, -6 ... Comparison of these inhibitors with the natural substrates of the enzyme, cAMP and cGMP (see figure 1 through 5) reveal some ... The structures of Bay 60-7550 and PDP are very similar (see figure 4 and 5). The difference between these molecules is the ...
5'---GCATG C---3' 3'---C GTACG---5' StuI*[76][77]. Streptomyces tubercidicus 5'AGGCCT 3'TCCGGA 5'---AGG CCT---3' 3'---TCC GGA ... ISBN 0-89603-234-5.. *^ a b Arber W, Linn S (1969). "DNA modification and restriction". Annual Review of Biochemistry. 38: 467- ... 5'---CAGCAGN25 NN---3' 3'---GTCGTCN25NN ---5' KpnI[75]. Klebsiella pneumoniae 5'GGTACC 3'CCATGG 5'---GGTAC C---3' 3'---C CATGG ... 5 (11): 2748-66. doi:10.3390/v5112748. PMC 3856413. PMID 24284874.. *^ Stetson DB, Ko JS, Heidmann T, Medzhitov R (Aug 2008). " ...
Dai Duy Ban and J.Kawiak: (Announcement in Poland in English): Nucleotidase of L 1210 Cells. Cytochemical and Kinetic Studies, ... Cell structure and Function 5, 211-215, 1980. Dai Duy Ban and Aleksandra Przetecka: (Announcement in Poland in English): ...
Angle, C. R.; McIntire, M. S. (1978). "Low level lead and inhibition of erythrocyte pyrimidine nucleotidase". Environmental ... 56 (5): 819-822. PMID 1196740. Angle, C. R.; McIntire, M. S.; Brunk, G. (1977). "Effect of anemia on blood and tissue lead in ... 5: 179-184. Angle, C. R.; Manton, W. I.; Stanek, K. L. (1995). "Stable isotope identification of lead sources in preschool ... 5 (5): 855-870. doi:10.1080/15287397909529795. PMID 583166. Angle, C. R.; Stohs, S. J.; McIntire, M. S.; Swanson, M. S.; Rovang ...
... nucleotidase), PITPNM2 (Membrane-associated phosphatidylinositol transfer protein 2), SBNO1 (strawberry notch homolog 1), ZEB2 ... 31 (5): 864-70. doi:10.1093/carcin/bgq051. PMC 2864416. PMID 20197299. Liu M, Lang N, Qiu M, Xu F, Li Q, Tang Q, Chen J, Chen X ... 68 (5): 1362-8. doi:10.1158/0008-5472.CAN-07-2912. PMID 18316599. Smrt RD, Szulwach KE, Pfeiffer RL, Li X, Guo W, Pathania M, ... 2 (5): 1233-1243. doi:10.1016/j.celrep.2012.09.033. ISSN 2211-1247. PMID 23168260. Landgraf P, Rusu M, Sheridan R, et al. (June ...
The nucleotidase and fosfatase incubation resulted in a reduction of the cytotoxicity of FdUMP, which means that the drug can ... The inhibition of the nucleotidase and fosfatase activity, decreased the effect of FdUMP, while the inhibitory effect was ... For 5-FU to inhibit TS by its mechanism of action, it must first be bioactivated through a series of reactions: 5-FU ends up ... Once the 5-FU or 5-FUdR prodrugs have been bioactivated resulting in FdUMP, they will already be recognized by the TS enzyme. ...
... then a nucleotidase creates inosine Purine nucleoside phosphorylase acts upon inosine to create hypoxanthine Xanthine oxidase ... catalyzes the oxidation of xanthine to uric acid A nuclease frees the nucleotide A nucleotidase creates adenosine, then ... A nuclease frees the nucleotide A nucleotidase creates guanosine Purine nucleoside phosphorylase converts guanosine to guanine ... 271 (14): 8192-5. doi:10.1074/jbc.271.14.8192. PMID 8626510. Garrett RH, Grisham CM (2016-02-11). Biochemistry (Sixth ed.). ...
... nucleotidase (5-NT) is a protein produced by the liver. A test can be done to measure the amount of this protein in your blood ... nucleotidase (5-NT) is a protein produced by the liver. A test can be done to measure the amount of this protein in your blood ...
... nucleotidase, thimidine monophosphate nucleotidase, 5-AMPase, 5-AMP nucleotidase, AMP phosphohydrolase, IMP 5-nucleotidase ... Reis, Julian (1934). "Nucleotidase and its relation to the deamination of nucleotides in the heart and the muscles". Bulletin ... Nucleotidase Levels Blood Test. Ecto-5Nucleotidase is considered a maturation marker for T cells and B cells. This is due to ... nucleotidase and has an extracellular location. The two cytosolic forms of the enzyme have similar characteristics, but can be ...
Nucleotidase-positive and-negative subpopulations, J.Clin.Invest. 56: 1324-1327 (1975).PubMedCrossRefGoogle Scholar ... Nucleotidase as a Leukemia Marker. In: De Bruyn C.H.M.M., Simmonds H.A., Müller M.M. (eds) Purine Metabolism in Man-IV. ... Nucleotidase (5′-N) as an ectoenzyme has the double advantage of being testable by an ordinary enzyme assay on intact cells and ... We have characterized leukemic cells using 5′-N not as an isolated marker but rather within a battery of other markers ...
... nucleotidase. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and definitions. ... nucleotidase. Definition: an enzyme that catalyzes the hydrolysis of a pyrimidine-nucleoside 5-monophosphate to produce ...
Other names in common use include phosphoadenylate 3-nucleotidase, 3-phosphoadenylylsulfate 3-phosphatase, phosphoadenylate ... bisphosphate nucleotidase (EC is an enzyme that catalyzes the chemical reaction adenosine 3,5-bisphosphate + H2O ... Bisphosphate nucleotidase". J. Biol. Chem. 262 (21): 10044-7. PMID 3038862. Lik-Shing Tsang M, Schiff JA (1976). "Properties of ... 3-nucleotidase, and 3(2),5-bisphosphonucleoside 3(2)-phosphohydrolase. This enzyme participates in sulfur metabolism. As ...
Nucleotidase (5NT) is an intrinsic membrane glycoprotein that is present as an enzyme in a wide variety of mammalian cells. It ... facilitates the hydrolysis of the phosphate group from 5-nucleotides, resulting in corresponding nucleosides. ... Nucleotidase (5NT) is an intrinsic membrane glycoprotein that is present as an enzyme in a wide variety of mammalian cells. [1 ... Nucleotidase (5NT) is an intrinsic membrane glycoprotein that is present as an enzyme in a wide variety of mammalian cells. [1 ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
5 p.m.. ET, Monday - Friday Email: [email protected] Phone: +1-800-860-8747 TTY: +1-866-569-1162 Chat. Live Chat ... Nucleotidase (CD73). Novel, small molecule nucleotide derivatives have been developed containing either a purine or pyrimidine ... nucleotidase. CD73 converts extracellular AMP to adenosine, the native activator of 4 subtypes of adenosine receptors. CD73 has ... nucleobase that competitively block the enzyme CD73, or ecto-5- ...
... nucleotidase of guinea pig polymorphonuclear leukocytes is localized exclusively on the plasma membrane of intact cells. The ...
Nucleotidase, C-terminal domain superfamily (IPR036907). Showing 1 to 20 of 355 results ...
analysis of Drosophila and human 7-methyl GMP-specific nucleotidases Title: Identification of Drosophila and human 7-methyl GMP ... nucleotidase, cytosolic IIIAprovided by HGNC. Primary source. HGNC:HGNC:17820 See related. Ensembl:ENSG00000122643 MIM:606224 ... nucleotidase 3. lupin. pyrimidine 5-nucleotidase 1. uridine 5-monophosphate hydrolase 1. NP_001002009.1. *EC ... nucleotidase NT5C3A is a negative epigenetic factor in interferon and cytokine signaling. Al-Haj L, et al. Sci Signal, 2018 Feb ...
... nucleotidase, ectoprovided by RGD. Primary source. RGD:61956 See related. Ensembl:ENSRNOG00000011071 Gene type. protein coding ... nucleotidase family [Nucleotide transport and metabolism, Defense mechanisms]. cd07409. Location:31 → 312. MPP_CD73_N; CD73 ... nucleotidase. Names. 5-NT. ecto-5-nucleotidase. NP_067587.2. *EC ... nucleotidase and related proteins, N-terminal metallophosphatase domain. pfam02872. Location:341 → 509. 5_nucleotid_C; 5- ...
... nucleotidase (5N) (E.C.- is present in lymphocytes isolated from the blood of normal subjects. This activity is ... Citation Information: J Clin Invest. 1975;56(5):1324-1327. https://doi.org/10.1172/JCI108209. View: Text , PDF ...
Nucleotidase with a broad substrate specificity as it can dephosphorylate various ribo- and deoxyribonucleoside 5- ...
... nucleotidase phosphodiesterase hereditary degeneration of the retina Laboratory of Biochemical Bases of Reception, I. M. ... nucleotidase activity in heriditary degeneration of the retina. ... 5.. A. L. Berman, A. M. Azimova, and F. G. Gribakin, Vision Res ...
Nucleotidase/CD73 products for your research including 5-Nucleotidase/CD73 Primary Antibodies, Proteins and Enzymes, Small ... Nucleotidase/CD73: Products. CD73, an ecto-5-prime-nucleotidase, is a glycosyl phophatidylinositol-anchored ectoenzyme that ... The 5-prime-nucleotidase activity of CD73 converts extracellular nucleoside 5-prime monophosphates to nucleosides. It is one of ... Adenosine 5-(α,β-methylene)diphosphate sodium salt Ecto-5-nucleotidase (CD73) inhibitor ...
... nucleotidase. Mouse monoclonal antibody clone AD2 is used for FC. ... nucleotidase expressed on the surface of subsets of B and T ... CD73 is a glycosyl phosphatidylinositol-anchored glycoprotein and ecto-5- ... Nucleotidase,human antibody Ecto-5-Nucleotidase AD2,human antibody Ect ... Figure 5. Data for PE-Conjugated. (A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on ...
Nucleotidase with a broad substrate specificity as it can dephosphorylate various ribo- and deoxyribonucleoside 5- ... Belongs to the SurE nucleotidase family.UniRule annotation. ,p>Manual validated information which has been generated by the ... Nucleotidase with a broad substrate specificity as it can dephosphorylate various ribo- and deoxyribonucleoside 5- ... nucleotidase SurEUniRule annotation. ,p>Manual validated information which has been generated by the UniProtKB automatic ...
... nucleotidase/CD73 (e-5′NT/CD73), the enzyme responsible for adenosine formation from AMP. e-5′NT/CD73-/- mice were viable and ... e-5. ′. NT/CD73. mRNA transcripts in e-5. ′. NT/CD73+/+. (WT) and e-5. ′. NT/CD73-/-. mice (KO). Primer sequences are located ... e-5′NT/CD73-/- mice had a significantly reduced fall in stop flow pressure and superficial nephron glomerular filtration rate ... Blood pressure, blood and urine chemistry, and renal blood flow were not different between e-5′NT/CD73+/+ and e-5′NT/CD73-/- ...
... nucleotidase activity (ortholog); nucleotidase activity (ortholog); INVOLVED IN dCMP catabolic process (ortholog); ... nucleotidase activity (ortholog); nucleotidase activity (ortholog); INVOLVED IN dCMP catabolic process (ortholog); ... nucleotidase activity enables. ISO. RGD:1353894. 9068941. PMID:10681516. UniProt. PMID:10681516. ... nucleotidase activity enables. ISO. RGD:1556950. 9068941. MGI:55510, PMID:6301357. MGI. MGI:55510, PMID:6301357. ...
... nucleotidase, cytosolic IAImported. ,p>Information which has been imported from another database using automatic procedures.,/p ... nucleotidase, cytosolic IA OS=Xenopus tropicalis OX=8364 GN=nt5c1a PE=2 SV=1 ... Last modified:July 5, 2004 - v1. ,p>The checksum is a form of redundancy check that is calculated from the sequence. It is ... Annotation score:1 out of 5. ,p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB ...
... nucleotidase (e5′n; 5 kU/ml) increased adenosine production from ATP (30 μm) in a slice from a CD73−/− mouse. D, Application of ... nucleotidase. Because CD73−/− mice can still metabolize ATP to AMP (deletion of the CD73 gene has no effect on brain ectoATPase ... nucleotidase (5 kU/ml) had little effect on adenosine production in a slice from a CD73+/+ mouse. E, Graph summarizing ... nucleotidase; n = 4-5). **p , 0.01, Students t test. F, Graph plotting the amplitude of individual normalized parallel fiber ...
Nucleotidase/CD73 Antibody (AD2) cited in 15 publications. Validated: Flow, Flow-CS, IHC-Fr. Tested Reactivity: Human. ... Nucleotidase/CD73.. Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target. By Yoskaly Lazo-Fernandez, ... Nucleotidase/CD73 Antibody (NBP2-48480). (Showing 1 - 1 of 1 FAQs).. * We are interested in CD73 antibodies that could react ... nucleotidase (CD73) in normal mammary gland and in breast carcinoma. Br J Cancer. 1991 Jan 1 [PMID: 1989648] (IHC-Fr, Human). ...
Nucleotidase/CD73 Antibody (45M3B4) [HRP]. Validated: WB. Tested Reactivity: Human, Mouse, Rat, and more. 100% Guaranteed. ... Nucleotidase/CD73 Antibody (45M3B4) [HRP] Summary. Immunogen. Within the range of amino acids 169-324 of human CD73 were used ... Nucleotidase/CD73.. Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target. By Yoskaly Lazo-Fernandez, ... Nucleotidase/CD73 Antibody (NBP2-25235H). (Showing 1 - 1 of 1 FAQ).. * We are interested in CD73 antibodies that could react ...
... nucleotidase promotes invasion, migration and adhesion of human breast cancer cells J Cancer Res Clin Oncol. 2008 Mar;134(3): ... nucleotidase (eN, ecto-5-NT, CD73) generated extracellular adenosine in biologically malignant behaviors of human breast cancer ... The purpose of the present study was to evaluate the role of ecto-5- ...
One factor may be the level of AMP-hydrolyzing activity attributed to enzymes other than CD73 (i.e., other nucleotidases and/or ... Nucleotidase (CD73) in Vascular Leakage during Hypoxia. Linda F. Thompson, Holger K. Eltzschig, Juan C. Ibla, C. Justin Van De ... nucleotidase activity and cell surface expression in endothelial cells: role of membrane lipids. Circ. Res. 92:848-855. ... Nucleotidase from smooth muscle of small intestine and from brain. Inhibition by nucleotides. Biochemistry. 14:2362-2366. ...
... nucleotidase from Torpedo electric organ E J M Grondal E J M Grondal ... Nucleotidase was isolated from the electric organ of the electric ray Torpedo marmorata after solubilization in Triton X-100 ... nucleotidase from electric-ray ( Torpedo marmorata ) electric organ and bovine cerebral cortex is derived from the glycosyl- ... nucleotidase from Torpedo marmorata (electric ray) electric organ Biochem J (August, 1991) ...
... nucleotidase) Antibody - CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ... nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5-NT Isotype Mouse IgG1, κ Ave. Rating Submit a Review Product Citations publications ... nucleotidase, 70 kD Distribution Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, ... nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, ...
... bisphosphate nucleotidase 1 (HMDBP00342). IdentificationBiological propertiesGene propertiesProtein propertiesExternal links ... nucleotidase inhibited by inositol 1,4-bisphosphate. J Biol Chem. 1999 May 7;274(19):13619-28. [PubMed:10224133 ] ... Adenosine 3,5-diphosphate + Water → Adenosine monophosphate + Phosphate. details Phosphoadenosine phosphosulfate + Water → ... Converts adenosine 3-phosphate 5-phosphosulfate (PAPS) to adenosine 5-phosphosulfate (APS) and 3(2)-phosphoadenosine 5- ...
... nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain family. Additional information, provided for both this family and ... nucleotidase activity. 0. 2.376. --. DIRECT. Molecular Function (MF). phosphoric ester hydrolase activity. 0. 1.406. --. DIRECT ... 3-nucleotidase. 0.0000001668. 2.264. --. DIRECT. Enzyme Commission (EC). Acting on ester bonds. 0.0000000001597. 0.5107. Least ... nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain family. SCOP classification Root: SCOP hierarchy in SUPERFAMILY [. 0 ...
  • Novel, small molecule nucleotide derivatives have been developed containing either a purine or pyrimidine nucleobase that competitively block the enzyme CD73, or ecto-5'-nucleotidase. (nih.gov)
  • The encoded protein is the type 1 isozyme of pyrimidine 5' nucleotidase and catalyzes the dephosphorylation of pyrimidine 5' monophosphates. (nih.gov)
  • A New Homozygous Mutation (c.393-394del TA/c.393-394del TA) in the NT5C3 Gene Associated With Pyrimidine-5'-Nucleotidase Deficiency: A Case Report. (nih.gov)
  • The intracellular pyrimidine 5'-nucleotidase NT5C3A is a negative epigenetic factor in interferon and cytokine signaling. (nih.gov)
  • Pyrimidine-5'-nucleotidase Campinas, a new mutation (p.R56G) in the NT5C3 gene associated with pyrimidine-5'-nucleotidase type I deficiency and influence of Gilbert's Syndrome on clinical expression. (nih.gov)
  • New missense homozygous mutation (Q270Ter) in the pyrimidine 5' nucleotidase type I-related gene in two Indian families with hereditary non-spherocytic hemolytic anemia. (nih.gov)
  • Hemolytic anemia caused by pyrimidine 5'-nucleotidase (P5N) deficiency 15 years later. (biomedsearch.com)
  • Structure of pyrimidine 5'-nucleotidase type 1. (nih.gov)
  • Eukaryotic pyrimidine 5'-nucleotidase type 1 (P5N-1) catalyzes dephosphorylation of pyrimidine 5'-mononucleotides. (nih.gov)
  • s case of pyrimidine 5'-nucleotidase deficiency and heterozygous [alpha]-thalassemia was an interesting co-existence (1). (thefreedictionary.com)
  • Co-Existence of Hereditary Pyrimidine 5'-Nucleotidase Deficiency and Heterozygous a-Thalassemia: A Case Presentation. (thefreedictionary.com)
  • A case of hemolytic anemia due to erythrocyte pyrimidine 5'-nucleotidase deficiency. (thefreedictionary.com)
  • Detection of pyrimidine 5'-nucleotidase deficiency using [sup. (thefreedictionary.com)
  • Lead inhibits heme biosynthesis and causes anemia, basophilic stippling, a decline in red cell 8-aminolevulinic acid dehydrogenase, and an increase in urinary 8-aminolevulinic acid, urinary coproporphyrin, red cell zinc protoporphyrin (ZPP), and pyrimidine 5'-nucleotidase (12). (thefreedictionary.com)
  • The pyrimidine mononucleotides can be dephosphorylated by pyrimidine 5'-nucleotidase (P5N-I). In humans, a lack of this enzyme causes hemolytic anemia with ribosomal structures and trinucleotides retained in the red blood cells (RBCs). (uni-regensburg.de)
  • Distribution of erythrocyte nucleotides in pyrimidine 5'-nucleotidase deficiency. (wizdom.ai)
  • May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. (nih.gov)
  • We present 2 Norwegian siblings who we believe to be the first cases of pyrimidine 5'-nucleotidase deficiency diagnosed in the Scandinavian countries. (symptoma.com)
  • Abstract Two siblings with chronic hemolytic anemia due to red cell pyrimidine-5'-nucleotidase (P-5'-N) deficiency, presented within a few days of each other with a febrile illness and pancytopenia. (symptoma.com)
  • mdN and cdN are mitochondrial and cytosolic 5'-3' pyrimidine nucleotidases. (thefullwiki.org)
  • cN-III is a pyrimidine 5' nucleotidase. (thefullwiki.org)
  • Alternative splicing of human NT5E in cirrhosis and hepatocellular carcinoma produces a negative regulator of ecto-5'-nucleotidase (CD73). (medscape.com)
  • 5' Nucleotidase (Ecto 5' Nucleotidase or CD73 or NT5E or EC pipeline Target constitutes close to 22 molecules. (marketresearch.com)
  • The latest report 5' Nucleotidase - Pipeline Review, H1 2019, outlays comprehensive information on the 5' Nucleotidase (Ecto 5' Nucleotidase or CD73 or NT5E or EC targeted therapeutics, complete with analysis by indications, stage of development, mechanism of action (MoA), route of administration (RoA) and molecule type. (marketresearch.com)
  • Furthermore, this report also reviews key players involved in 5' Nucleotidase (Ecto 5' Nucleotidase or CD73 or NT5E or EC targeted therapeutics development with respective active and dormant or discontinued projects. (marketresearch.com)
  • We identified a cell surface ecto-5′-nucleotidase (Nt5e), as a candidate virulence factor. (umn.edu)
  • Ecto-5' -Nucleotidase CD73 (NT5E), vitamin D receptor and FGF23 gene polymorphisms may play a role in the development of calcific uremic arteriolop. (cdc.gov)
  • Gutensohn W., Thiel E., Buschette S. (1984) Ecto-5′-Nucleotidase as a Leukemia Marker. (springer.com)
  • The test may also be referred to as 5'NT Levels Blood Test, CDF73 Levels Blood Test, and Ecto-5'-Nucleotidase Levels Blood Test. (wikipedia.org)
  • The role of ecto-5'-nucleotidase in endothelial dysfunction and vascular pathologies. (medscape.com)
  • Holmes AP, Nunes AR, Cann MJ, Kumar P. Ecto-5'-Nucleotidase, Adenosine and Transmembrane Adenylyl Cyclase Signalling Regulate Basal Carotid Body Chemoafferent Outflow and Establish the Sensitivity to Hypercapnia. (medscape.com)
  • Wolff G, Truse R, Decking U. Extracellular Adenosine Formation by Ecto-5'-Nucleotidase (CD73) Is No Essential Trigger for Early Phase Ischemic Preconditioning. (medscape.com)
  • Fluvastatin, an HMG-CoA reductase inhibitor, facilitate adenosine production in the rat hearts via activation of ecto-5'-nucleotidase. (nih.gov)
  • Increased levels of adenosine and ecto 5'-nucleotidase (CD73) activity precede renal alterations in experimental diabetic rats. (nih.gov)
  • Fluvastatin increases adenosine concentrations in the heart ventricles as a result of activation of PKC, mediated by stimulation of alpha1-adrenoceptors, through activation of ecto-5'-nucleotidase. (nih.gov)
  • CD73, an ecto-5-prime-nucleotidase, is a glycosyl phophatidylinositol-anchored ectoenzyme that forms a disulfide-linked homodimer. (rndsystems.com)
  • The AD2 antibody reacts with human CD73, a glycosyl phosphatidylinositol (GPI)-anchored glycoprotein and ecto-5'-nucleotidase expressed on the surface of subsets of B and T cells, follicular dendritic cells, mesenchymal stem cells, endothelial cells, and epithelial cells. (stemcell.com)
  • To determine whether adenosine formation depends on extracellular nucleotide hydrolysis, we studied NaCl-dependent GFR regulation (tubuloglomerular feedback) in mice with targeted deletion of ecto-5′-nucleotidase/CD73 (e-5′NT/CD73), the enzyme responsible for adenosine formation from AMP. (jci.org)
  • Here, we have used mice that lack the CD73 gene (encoding ecto-5′-nucleotidase that converts AMP to adenosine) to test whether action potential-dependent adenosine release in the cerebellum depends on prior ATP release. (jneurosci.org)
  • Here, we have used mice that lack ecto-5′-nucleotidase ( CD73 ), a key enzyme required for the conversion of ATP to adenosine, to determine the mechanism of adenosine release and whether it arises from prior release of ATP. (jneurosci.org)
  • The purpose of the present study was to evaluate the role of ecto-5'-nucleotidase (eN, ecto-5-NT, CD73) generated extracellular adenosine in biologically malignant behaviors of human breast cancer cell lines. (nih.gov)
  • The generation of extracellular adenosine involves phosphohydrolysis of adenine nucleotide intermediates, and is regulated by the terminal enzymatic step catalyzed by ecto-5′-nucleotidase (CD73). (rupress.org)
  • Ecto-5′-nucleotidase (5′-NT, CD73) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates ( 14 ). (rupress.org)
  • CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. (biolegend.com)
  • Several new biomarkers were identified with altered expression in aneuploid cells, including overexpression of the cellular prion protein CD230/PrP C and the immunosuppressive cell surface enzyme ecto-5′-nucleotidase CD73. (aacrjournals.org)
  • Two main enzymes, cytosolic 5'-nucleotidase II (cN-II) and ecto-5'-nucleotidase (CD73) have been identified as main therapeutic targets in cancer. (chemaxon.com)
  • Ecto-5′-nucleotidase (CD73) is an immunosuppressive molecule intricately involved in adaptive and innate immune responses and is able to dephosphorylate AMP to adenosine. (biomedcentral.com)
  • This specific enzyme function has evolved in blood-feeding parasites from the ecto-ATPdase/CD39 (E-NTPDases)-, Cimex-type- and 5'-nucleotidase/CD73 enzyme families. (up.ac.za)
  • Molecular modelling of these two proteins showed a similar protein structure to a periplasmic ecto-5'-nucleotidase from E. coli. (up.ac.za)
  • Ecto-5'-nucleotidase (E5'N) is an endothelial surface enzyme that controls conversion of extracellular nucleotides into immunosuppressive adenosine. (unimib.it)
  • The involvement of ecto-5′-nucleotidase (E-5′Nu) in the elevation of extracellular adenosine during inflammation is unclear. (hku.hk)
  • To investigate the role of adenosine formed extracellularly in vascular homeostasis, mice with a targeted deletion of the cd73/ecto-5′- nucleotidase were generated. (elsevier.com)
  • Ectonucleotidases tested were ectonucleoside triphosphate diphosphohydrolase 1-3 (NTPDase1-3) and ecto-5-nucleotidase (eN), whereas the effects were evaluated in two brain areas that show different sensitivity to glucocorticoid action, hippocampus, and cerebral cortex. (ac.rs)
  • We investigated 10 target genes using a tagging SNP approach: the genes encoding CD73/ ecto-5'-nucleotidase (purinergic pathway), Matrix Gla protein, Fetuin A, Bone Gla protein, VKORC1 (all related to intrinsic calcification inhibition), calcium-sensing receptor, FGF23, Klotho, vitamin D receptor, stanniocalcin 1 (all related to CKD-MBD). (cdc.gov)
  • The last and rate-limiting step of the hydrolysis is catalyzed by membrane-bound ecto-50-nucleotidase (eN). (ac.rs)
  • Mutations in this gene are a cause of hemolytic anemia due to uridine 5-prime monophosphate hydrolase deficiency. (nih.gov)
  • In lead poisoning, anemia, basophilic stippling and inhibition of erythrocyte pyrimidin 5' deficiency are also observed. (biomedsearch.com)
  • In the present work, we report two cases of hemolytic anemia secondary to congenital deficiency of pyrimidin 5' nucleotidase and another case secondary to lead poisoning. (biomedsearch.com)
  • Since 1974, when pyrimidin 5' nucleotidase deficiency was isolated, is known that hemolysis is related to the accumulation of pyrimidin nucleotides within the erythrocytes that behave as metabolic inhibitors. (biomedsearch.com)
  • 5'-Nucleotidase activity in retinol deficiency induced albino rats. (bvsalud.org)
  • This gene encodes a member of the 5'-nucleotidase family of enzymes that catalyze the dephosphorylation of nucleoside 5'-monophosphates. (nih.gov)
  • We conclude that the generation of adenosine at the glomerular pole depends to a major extent on e-5′NT/CD73-mediated dephosphorylation of 5′-AMP, presumably generated from released ATP. (jci.org)
  • Their dephosphorylation by 5'NTs might be one of the mechanisms behind the resistance experienced by patients towards such drugs.This thesis describes structure-function studies on four of the seven known human 5'-NTs. (openthesis.org)
  • However, cytoplasmic 5′-nucleotidases catalyze dephosphorylation of ara-CMP, thereby reducing the amount of ara-C for conversion to ara-CTP ( Amici and Magni, 2002 ). (aspetjournals.org)
  • also known as cN-II and high K m 5′-nucleotidase) and cytoplasmic 5′-nucleotidase III (NT5C3) are involved in the final step of dephosphorylation before export of nucleosides out of the cell. (aspetjournals.org)
  • This gene encodes a nucleotidase that catalyzes the dephosphorylation of the 5' deoxyribonucleotides (dNTP) and 2'(3')-dNTP and ribonucleotides, but not 5' ribonucleotides. (genecards.org)
  • In mammalian cells the enzyme is predominantly located in the plasma membrane and its primary role is in the conversion of extracellular nucleotides (e.g. 5'-AMP), which are generally impermeable, to the corresponding nucleoside (e.g. adenosine) which can readily enter most cells. (wikipedia.org)
  • The 5-prime-nucleotidase activity of CD73 converts extracellular nucleoside 5-prime monophosphates to nucleosides. (rndsystems.com)
  • CD73 catalyzes the hydrolysis of 5'-adenosine monophosphate (AMP) to form the bioactive nucleoside adenosine, and plays a pivotal role in the activation of P1 adenosine receptors by regulating extracellular adenosine concentrations. (stemcell.com)
  • In humans, eight 5'-nucleotidases catalyze the hydrolysis of nucleoside 5'-monophosphates into their respective nucleosides in order to regulate the intracellular / extracellular nucleotide pools. (chemaxon.com)
  • Taking advantage of complete genome sequencing of Saccharomyces cerevisiae we were able to identify several new purine salvage genes such as adenosine kinase or purine nucleoside phosphorylase [ 4 , 5 ] which are highly homologous to their mammalian counterpart. (biomedcentral.com)
  • A nucleotidase is a hydrolytic enzyme that catalyzes the hydrolysis of a nucleotide into a nucleoside and a phosphate . (thefullwiki.org)
  • 5'-Nucleotidases (5'NTs) are catabolic enzymes of the nucleotide metabolism. (openthesis.org)
  • These results represent the first confirmation of a tick apyrase that belongs to the 5'-nucleotidase family of enzymes. (up.ac.za)
  • Lipid peroxidation is followed by loss of structural integrity of plasma membranes thereby releasing the membrane associated enzymes like Adenosine deaminase and 5'-nucleotidase into the circulation. (thefreedictionary.com)
  • Electric ray 5′‐nucleotidase shares 61% amino acid identity with the enzymes from rat liver and human placenta, and about 23% with bacterial proteins possessing 5′‐nucleotidase activity and also additional enzyme activities like UDP‐glucose hydrolase. (elsevier.com)
  • Interestingly, there are 5-7 domains highly conserved in procaryotes and vertebrates in enzymes exhibiting 5′‐nucleotidase, 3′‐nucleotidase or phosphodiesterase activity. (elsevier.com)
  • Enzymes adenosine deaminase (ADA) and 5-nucleotidase (5-'NT) are known to play active role in tissue / cell proliferation and differentiation. (bvsalud.org)
  • Soluble 5' nucleotidases are all known to belong to the haloacid dehalogenase superfamily of enzymes which are two domian proteins characterised by a modified Rossman fold as the core and variable cap or hood. (thefullwiki.org)
  • This AD2 monoclonal CD73 antibody reacts with human CD73, a 5'-ectonucleotidase that converts 5'-adenosine monophosphate to adenosine. (novusbio.com)
  • There are currently no images for 5'-Nucleotidase/CD73 Antibody (NBP2-25235H). (novusbio.com)
  • Immunocytochemistry of frozen thin sections with either single or double layers of antibody and visualized with protein A complexed with 5 nm colloidal gold was used to estimate the same density. (gla.ac.uk)
  • Nucleotidase/CD73 Monoclonal antibody specifically detects 5 ft. (fishersci.com)
  • In order to evaluate the effect of the surface modification on 5'-nucleotidase (5eNT) separation, magnetite with no modification (M), silica-magnetite (MS), silica-aminosilane-magnetite (MSA), silica-amino silane-ScFv antibody-magnetite (MSACF), and silica-aminosilane-anti-CD73 antibody (MSA-CD73) were tested. (thefreedictionary.com)
  • Orthologues of Streptococcus pyogenes nuclease A (SpnA) and Streptococcal 5′-nucleotidase A... Soh, Kar Yan;Loh, Jacelyn Mei San;Proft, Thomas 2018-04-05 00:00:00 Abstract Streptococcus pyogenes nuclease A (SpnA) and streptococcal 5′ nucleosidase A (S5nA) are two recently described virulence factors from the human pathogen S. pyogenes. (deepdyve.com)
  • The enzyme has a wide substrate specificity for nucleotides and has been shown to hydrolyze 5'nucleotides rapidly, ribose-5-phosphate slowly, and other phosphate esters extremely slowly (if at all). (wikipedia.org)
  • [ 1 ] It facilitates the hydrolysis of the phosphate group from 5'-nucleotides, resulting in corresponding nucleosides. (medscape.com)
  • In enzymology, a 3'(2'),5'-bisphosphate nucleotidase (EC is an enzyme that catalyzes the chemical reaction adenosine 3',5'-bisphosphate + H2O ⇌ {\displaystyle \rightleftharpoons } adenosine 5'-phosphate + phosphate Thus, the two substrates of this enzyme are adenosine 3',5'-bisphosphate and H2O, whereas its two products are adenosine 5'-phosphate and phosphate. (wikipedia.org)
  • A 5'-ribonucleotide + H 2 O = a ribonucleoside + phosphate. (uniprot.org)
  • Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. (hmdb.ca)
  • This enzyme catalyses the following chemical reaction:a 5'-ribonucleotide + H2O↔ a ribonucleoside + phosphate. (creative-enzymes.com)
  • Yeast3',5'-bisphosphate nucleotidase (YND), also known as HAL2, can hydrolyze3'-phosphate from PAP to form AMP or from PAPS to form APS, had been found involved in sulfur metabolism and salt stress. (globethesis.com)
  • 5' nucleotidases cleave off the phosphate from the 5' end of the sugar moiety. (thefullwiki.org)
  • 5'-nucleotidase (5'-NT) is a protein produced by the liver. (medlineplus.gov)
  • Cloning of a mouse cytosolic 5'-nucleotidase-I identifies a new gene related to human autoimmune infertility-related protein. (nih.gov)
  • The high affinity of ND to PAP and commercial availability for PAP agarose (the cross-linking product of activated agarose and the free amino group from N6-[(6-amino)octyl]-amino-adenosine-3',5'-bisphosphate, Sigma-aldrich,#A3640) enabled affinity purification of YND which suggested YND could be used as a possible fusion affinity tag for protein purification.Affinity chromatography is one of the most popular methods for protein purification. (globethesis.com)
  • NT5C (5', 3'-Nucleotidase, Cytosolic) is a Protein Coding gene. (genecards.org)
  • LPS upregulated the protein expression but not the messenger RNA expression of E-5′Nu. (hku.hk)
  • Therefore, the present study reports that the activity and protein abundance of membrane-bound eN fluctuates across the estrus cycle in the hippocampal synaptosomes of female rats. (ac.rs)
  • Selective ERa receptor agonist, PPT, induced upregulation of both the protein level and activity of eN, while application of selective ERb receptor agonist, DPN, increased only the activity of eN. (ac.rs)
  • The enzyme catalyzes the hydrolysis of a 5'-ribonucleotide to a ribonucleoside and orthophosphate in the presence of water. (uchicago.edu)
  • 5′‐nucleotidase isolated from Torpedo electric organ hydrolyzes UDP‐glucose at 8% of the rate of AMP hydrolysis. (elsevier.com)
  • Adenosine 5′-[alpha, beta-methylene]diphosphate, ADP and ATP are competitive inhibitors in this order of potency. (portlandpress.com)
  • Download the Allosteric inhibitors targeting 5'-nucleotidases to restore poster. (chemaxon.com)
  • The possible phylogenetic origin of vertebrate 5′‐nucleotidase from multifunctional nucleotide hydrolases is discussed. (elsevier.com)
  • This gene encodes a hydrolase that serves as an important role in cellular purine metabolism by acting primarily on inosine 5'-monophosphate and other purine nucleotides. (nih.gov)
  • Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides. (nih.gov)
  • The purine salvage enzyme inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase catalyzes degradation of IMP to inosine. (biomedcentral.com)
  • 5' nucleotidases are involved in varied functions like cell-cell communication, nucleic acid repair, purine salvage pathway for the synthesis of nucleotides, signal transduction, membrane transport etc. (thefullwiki.org)
  • Cytosolic 5′-nucleotidase II (NT5C2) is involved in the development of 1-β- d -arabinofuranosylcytosine (ara-C) resistance and has been associated with clinical outcome in patients receiving ara-C-based chemotherapy. (aspetjournals.org)
  • Of most interest, SNPs (linkage disequilibrium group CEU.12) in the 5′-untranslated region were associated with NT5C2 expression and ara-C sensitivity in HapMap cell lines and with NT5C2 mRNA expression and ara-C sensitivity in diagnostic leukemic blasts from pediatric patients with acute myeloid leukemia. (aspetjournals.org)
  • Previous genetic studies demonstrated that chemoresistant ALL is driven by activating mutations in NT5C2 , the gene encoding cytosolic 5´-nucleotidase (cN-II). (biomedcentral.com)
  • 5′-Nucleotidase (5NT) is an intrinsic membrane glycoprotein that is present as an enzyme in a wide variety of mammalian cells. (medscape.com)
  • Spiegelberg BD, Xiong JP, Smith JJ, Gu RF, York JD: Cloning and characterization of a mammalian lithium-sensitive bisphosphate 3'-nucleotidase inhibited by inositol 1,4-bisphosphate. (hmdb.ca)
  • 5'-nucleotidase is an intrinsic membrane glycoprotein, present as an ectoenzyme in a wide variety of mammalian cells and it hydrolyzes extracellular AMP to adenosine and represents the major control point for extracellular adenosine levels and is a regulator of the adenosine signaling pathway. (thefreedictionary.com)
  • Polyclonal antibodies raised against 5′‐nucleotidase from mammalian sources or the electric ray electric organ reveal mutual crossreactivity. (elsevier.com)
  • The crystal structure of 5'-nucleotidase (5'-NT) from E. coli, also known as UDP-sugar hydrolase, has been determined at 1.7 A resolution. (proteopedia.org)
  • Other names in common use include phosphoadenylate 3'-nucleotidase, 3'-phosphoadenylylsulfate 3'-phosphatase, phosphoadenylate 3'-nucleotidase, and 3'(2'),5'-bisphosphonucleoside 3'(2')-phosphohydrolase. (wikipedia.org)
  • Gene Ontology (GO) annotations related to this gene include phosphatase activity and nucleotidase activity . (genecards.org)
  • 5'-nucleotidase (EC is an enzyme which catalyzes the phosphorylytic cleavage of 5'nucleotides. (wikipedia.org)
  • One of the soluble forms appears to be derived from the GPI-anchored ex-5'-nucleotidase and has an extracellular location. (wikipedia.org)
  • Vascular leakage secondary to hypoxia was reversed in part by adenosine receptor agonists or reconstitution with soluble 5′-nucleotidase. (rupress.org)
  • The isoforms were predicted to be soluble secreted proteins, which correlated with the trend observed for parasitic apyrases in the 5'-nucleotidase family. (up.ac.za)
  • Nucleotidase with a broad substrate specificity as it can dephosphorylate various ribo- and deoxyribonucleoside 5'-monophosphates and ribonucleoside 3'-monophosphates with highest affinity to 3'-AMP. (rcsb.org)
  • Membrane bound 5' nucleotidases displays specificity towards adenosine monophosphates and are predominantly involved in the salvage of preformed nucleotides and in signal transduction cascades involving purinergic receptors. (thefullwiki.org)
  • Further, we analyzed the role of E2 and its intracellular receptors on the expression of eN in ovariectomized females. (ac.rs)
  • Previous findings obtained on adenosine metabolism in brain suggest that eN may be modulated by ovarian steroids. (ac.rs)
  • Strikingly, no 5'-nucleotidase encoding gene could be inferred from comparison of the completely sequenced yeast genome with 5'-nucleotidase genes from other species, although several lines of evidence argued for the presence of 5'-nucleotidase in yeast. (biomedcentral.com)
  • This is due to the fact that the enzyme activity is approximately 10-times higher for peripheral T cells than thymocytes, 5-6 times higher in adult peripheral B cells than fetal B cells, and largely absent in non-T cell and non-B cell lymphocytes. (wikipedia.org)
  • A cDNA coding for bovine cytosolic IMP/GMP-specific 5ʹ-nucleotidase endowed with phosphotransferase activity was cloned from calf thymus RNA, by 5ʹ and 3ʹ rapid amplification of cDNA ends protocols (5ʹ and 3ʹ RACE). (biochemj.org)
  • It exhibited both 5ʹ-nucleotidase and phosphotransferase activity, as well as immunological and kinetic properties similar to those of the enzyme purified from calf thymus. (biochemj.org)
  • Escherichia coli 5′-nucleotidase activity is stimulated 30- to 50-fold in vitro by the addition of Co 2+ . (biochemj.org)
  • The studies reported herein suggest that displacement occurs at the metal-ion-binding site consisting of residues Asp 84 , Asn 116 , His 217 and His 252 , leading to the observed increase in 5′-nucleotidase activity. (biochemj.org)
  • Dephosphorylates the 5 and 2(3)-phosphates of deoxyribonucleotides, with a preference for dUMP and dTMP, intermediate activity towards dGMP, and low activity towards dCMP and dAMP. (genecards.org)
  • E-5′Nu activity was enhanced after a 24 h exposure to LPS. (hku.hk)
  • At 10 μM, the phosphatidylinositol 3-kinase (PI3K) inhibitor LY-294002 abolished the LPS-induced E-5′Nu activity. (hku.hk)
  • In conclusion, the experiments indicate that LPS upregulates E-5′Nu activity in HUVECs through a PI3K-dependent increase in the abundance of E-5′Nu on cell membranes. (hku.hk)
  • Although this enzymatic activity has been purified and characterized in Saccharomyces cerevisiae , the gene encoding IMP 5'-nucleotidase had not been identified. (biomedcentral.com)
  • Knock-out of ISN1 resulted in the total loss of IMP-specific 5'-nucleotidase activity, thus confirming that the ISN1 gene indeed encodes the enzymatic activity purified from yeast. (biomedcentral.com)
  • We have identified a new yeast gene, ISN1 ( YOR155c ), as encoding IMP-specific 5'-nucleotidase activity. (biomedcentral.com)
  • The 5'-NT activity, however, did not show any marked difference between the two horns under Gr.I and II in both species. (bvsalud.org)
  • and (3) the activity of enzyme 5'-NT does not alter during the deciduoma formation in both the species. (bvsalud.org)
  • The similarity in the coding region to that of the human 5ʹ-nucleotidase cDNA sequence [Oka, Matsumoto, Hosokawa and Inoue (1994) Biochem. (biochemj.org)
  • The bovine cDNA sequence included the sequences codifying for six peptides obtained from 5ʹ-nucleotidase/phosphotransferase purified from calf thymus. (biochemj.org)
  • A cDNA encoding a 5′‐nucleotidase was identified by screening a λgt10 cDNA library from the electric lobe of Discopyge ommata using a cDNA probe containing the complete open reading frame coding for the rat liver enzyme. (elsevier.com)
  • Inactive variants of the mitochondrial and cytosolic deoxynucleotidases and the cytosolic 5'-nucleotidase II were used to characterize the structural basis for their substrate specificity in high detail.Based on structures of the apoprotein and activator/activator+substrate complexes of cytosolic 5'-nucleotidase II, a mechanism for the allosteric activation of this enzyme was presented. (openthesis.org)
  • Unlike other nucleotidases, it is subject to complex allosteric regulation. (biomedcentral.com)
  • 5'-NT creates adenosine from adenosine monophosphate (AMP). (dovemed.com)
  • One unit will hydrolyze 1.0 μmole of inorganic phosphorus from adenosine 5'-monophosphate per min at pH 9.0 at 37°C. (creative-enzymes.com)
  • The systematic name of this enzyme class is adenosine-3'(2'),5'-bisphosphate 3'(2')-phosphohydrolase. (wikipedia.org)
  • 5'-nucleotidase is an enzyme with system name 5'-ribonucleotide phosphohydrolase. (creative-enzymes.com)
  • Citations to Human lymphocytes: 5'-nucleotidase-positive and -negative subpopulations. (jci.org)
  • Human lymphocytes: 5'-nucleotidase-positive and -negative subpopulations. (jci.org)
  • The enzyme, 5'-nucleotidase (5'N) (E.C.- is present in lymphocytes isolated from the blood of normal subjects. (jci.org)
  • X-ray structure of the Escherichia coli periplasmic 5'-nucleotidase containing a dimetal catalytic site. (proteopedia.org)
  • The inhibition of E-5′Nu by 100 μM α,β-methylene adenosine-5′-diphosphate increased the LPS-induced inflammation, suggesting that E-5′Nu plays a significant role in reducing inflammation, probably through the generation of adenosine. (hku.hk)
  • Primary structure of human placental 5′-nucleotidase and identification of the glycolipid anchor in the mature form. (dovemed.com)
  • Nucleotidase/CD73 in Human samples. (fishersci.com)
  • In the present study, the effect of lipopolysaccharide (LPS), an inflammation inducer, was investigated on E-5′Nu in human umbilical vein endothelial cells (HUVECs). (hku.hk)
  • nucleotidase in human glioblastoma. (edu.pl)
  • Since adenosine is an anti-inflammatory molecule, E-5′Nu upregulation may be crucial in protecting endothelial cells against inflammatory damage. (hku.hk)
  • Piracetam prevents scopolamine-induced memory impairment and decrease of NTPDase, 5'-nucleotidase and adenosine deaminase activities," Neurochemical Research, vol. (thefreedictionary.com)
  • 5'-Nucleotidase (5'-NT) is an enzyme found in the liver. (dovemed.com)
  • 5'-NT appears in blood when the liver is experiencing problems. (dovemed.com)
  • The main cause for this rise in 5'-NT levels is blockage of the passageways connecting the liver, duodenum, and gallbladder. (dovemed.com)
  • This test measures the levels of 5'- nucleotidase (an enzyme specific to the liver). (rochester.edu)
  • The 5'- nucleotidase level is elevated in persons with liver diseases, especially those diseases associated with cholestasis. (rochester.edu)
  • Seven residues from conserved sequence motifs implicated in the catalytic and metal-ion-binding sites of E. coli 5′-nucleotidase (Asp 41 , His 43 , Asp 84 , His 117 , Glu 118 , His 217 and His 252 ) were selected for modification using site-directed mutagenesis of the cloned ushA gene. (biochemj.org)
  • The 5'-nucleotidase of guinea pig polymorphonuclear leukocytes is localized exclusively on the plasma membrane of intact cells. (sciencemag.org)
  • Several independent methods were used to determine the density of 5'-nucleotidase on the plasma membrane of the Fao cell. (gla.ac.uk)
  • Non-enzymatic functions of eN have also been reported. (edu.pl)
  • The function of 5'-NT is to create the molecule adenosine. (dovemed.com)
  • CD39 can also convert the latter molecule into AMP, fully dephosphorylated to ADO by the 5'-nucleotidase (5'-NT) CD73 [7]. (thefreedictionary.com)
  • Serum levels of 5′-nucleotidase in disease. (medscape.com)