A potent mutagen and carcinogen. This compound and its metabolite 4-HYDROXYAMINOQUINOLINE-1-OXIDE bind to nucleic acids. It inactivates bacteria but not bacteriophage.
Quinolines substituted in any position by one or more nitro groups.
Tumors or cancer of the TONGUE.
A potent mutagen and carcinogen. It is a reduction product of 4-NITROQUINOLINE-1-OXIDE. It binds with nucleic acids and inactivates both bacteria and bacteriophage.
Substances that increase the risk of NEOPLASMS in humans or animals. Both genotoxic chemicals, which affect DNA directly, and nongenotoxic chemicals, which induce neoplasms by other mechanism, are included.
Compounds having the nitro group, -NO2, attached to carbon. When attached to nitrogen they are nitramines and attached to oxygen they are NITRATES.
A muscular organ in the mouth that is covered with pink tissue called mucosa, tiny bumps called papillae, and thousands of taste buds. The tongue is anchored to the mouth and is vital for chewing, swallowing, and for speech.
A flavanone glycoside found in CITRUS fruit peels.
Chemical agents that increase the rate of genetic mutation by interfering with the function of nucleic acids. A clastogen is a specific mutagen that causes breaks in chromosomes.
A proteolytic enzyme obtained from Streptomyces griseus.
An NADPH-dependent enzyme that catalyzes the conversion of L-ARGININE and OXYGEN to produce CITRULLINE and NITRIC OXIDE.
Quinolines substituted in any position by one or more amino groups.
Tumors or cancer of the MOUTH.
A circumscribed benign epithelial tumor projecting from the surrounding surface; more precisely, a benign epithelial neoplasm consisting of villous or arborescent outgrowths of fibrovascular stroma covered by neoplastic cells. (Stedman, 25th ed)
Agents that reduce the frequency or rate of spontaneous or induced tumors independently of the mechanism involved.
A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed)
Pathological processes that tend eventually to become malignant. (From Dorland, 27th ed)
The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.
A CALCIUM-independent subtype of nitric oxide synthase that may play a role in immune function. It is an inducible enzyme whose expression is transcriptionally regulated by a variety of CYTOKINES.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
Binary compounds of oxygen containing the anion O(2-). The anion combines with metals to form alkaline oxides and non-metals to form acidic oxides.
A transferase that catalyzes the addition of aliphatic, aromatic, or heterocyclic FREE RADICALS as well as EPOXIDES and arene oxides to GLUTATHIONE. Addition takes place at the SULFUR. It also catalyzes the reduction of polyol nitrate by glutathione to polyol and nitrite.
Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.
That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.
A CALCIUM-dependent, constitutively-expressed form of nitric oxide synthase found primarily in ENDOTHELIAL CELLS.
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
A CALCIUM-dependent, constitutively-expressed form of nitric oxide synthase found primarily in NERVE TISSUE.
Compounds with a six membered aromatic ring containing NITROGEN. The saturated version is PIPERIDINES.
Inorganic oxides that contain nitrogen.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A mild astringent and topical protectant with some antiseptic action. It is also used in bandages, pastes, ointments, dental cements, and as a sunblock.
The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)
The physiologically active form of vitamin D. It is formed primarily in the kidney by enzymatic hydroxylation of 25-hydroxycholecalciferol (CALCIFEDIOL). Its production is stimulated by low blood calcium levels and parathyroid hormone. Calcitriol increases intestinal absorption of calcium and phosphorus, and in concert with parathyroid hormone increases bone resorption.
A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.
A yellow-orange dye obtained from tumeric, the powdered root of CURCUMA longa. It is used in the preparation of curcuma paper and the detection of boron. Curcumin appears to possess a spectrum of pharmacological properties, due primarily to its inhibitory effects on metabolic enzymes.
A type of mutation in which a number of NUCLEOTIDES deleted from or inserted into a protein coding sequence is not divisible by three, thereby causing an alteration in the READING FRAMES of the entire coding sequence downstream of the mutation. These mutations may be induced by certain types of MUTAGENS or may occur spontaneously.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
The systematic study of the complete complement of proteins (PROTEOME) of organisms.
The protein complement of an organism coded for by its genome.
Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.
Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms.
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.

Functional analysis of the promoter of the yeast SNQ2 gene encoding a multidrug resistance transporter that confers the resistance to 4-nitroquinoline N-oxide. (1/242)

The yeast gene SNQ2, which encodes a multidrug resistance ABC superfamily protein, is required for resistance to the mutagen 4-nitroquinoline N-oxide (4-NQO). The expression of the SNQ2 gene is under the control of a regulatory network that involves the transcription factor Yrr1p, as well as Pdr1p/Pdr3p (Cui et al., Mol. Microbiol., 29, 1307-1315 (1998)). By 5'-deletion analysis of the promoter by using SNQ2-lacZ fusion constructs, four regions: -745 to -639 (region I), -639 to -578 (region II), -548 to -533 (region III) and -533 to -485 (region IV) were found to be important for SNQ2 expression. Genetic analysis suggested that the site in region IV was responsible for the Yrr1p-mediated SNQ2 expression. A consensus motif known for the binding of Pdr1p/Pdr3p (PDRE) was not found in region IV.  (+info)

Inhibition of DNA replicon initiation by 4-nitroquinoline 1-oxide, adriamycin, and ethyleneimine. (2/242)

The effects of three widely differing chemical carcinogens, 4-nitroquinoline 1-oxide, Adriamycin, and ethyleneimine, on DNA replication were studied by pulse labeling of DNA with [3H]thymidine and sedimentation analysis with alkaline sucrose gradients. At doses that reduced the rate of DNA synthesis to 30 to 60% of control values, only ethyleneimine produced damage that resulted in lower molecular weights of parental DNA. All three chemicals inhibited replicon initiation, but to differing extents. Inhibition of replicon initiation was the first clearly identified effect of 4-nitroquinoline 1-oxide and was the main cause of inhibition of DNA synthesis. Ethyleneimine caused severe inhibition of replicon initiation, but blocks to chain elongation also contributed significantly to the inhibition of overall DNA synthesis. Adriamycin affected replicon initiation to a small but significant extent; the primary cause of inhibition of DNA synthesis by this drug was a slowing of the rate of chain elongation. These results indicate that inhibition of replicon initiation is an important mechanism for the action of DNA-damaging agents in mammalian cells and strengthen the concept that control of DNA replication depends on the structural integrity of a chromosomal subunit that consists of several replicons.  (+info)

Inverse correlation between p53 protein levels and DNA repair efficiency in human fibroblast strains treated with 4-nitroquinoline 1-oxide: evidence that lesions other than DNA strand breaks trigger the p53 response. (3/242)

Ionizing radiation-induced stabilization and the resultant transient accumulation of the p53 tumor suppressor protein is impaired in cells from ataxia telangiectasia (AT) patients, indicating a key role for ATM, the gene mutated in AT, upstream in the radiation-responsive p53 signaling pathway. Activation of this pathway is generally assumed to be triggered by DNA strand breaks produced directly following genotoxic stress or indirectly during excision repair of DNA lesions. The aim of this study was to identify the triggering signal for induction of p53 in diploid human dermal fibroblasts treated with 4-nitroquinoline 1-oxide (4NQO), a model environmental carcinogen that produces both DNA strand breaks (like ionizing radiation) and alkali-stable bulky DNA lesions (like UV light). 4NQO treatment of fibroblasts cultured from normal and AT donors and those from patients with the UV-hypersensitivity disorder xeroderma pigmentosum (XP, complementation groups A, E and G) resulted in up-regulation of p53 protein. In normal fibroblasts, there was no temporal relationship between the incidence of DNA strand breaks and levels of p53 protein; >90% of strand breaks and alkali-labile sites were repaired over 2 h following treatment with 1 microM 4NQO, whereas approximately 3 h of post-treatment incubation was required to demonstrate a significant rise in p53 protein. In contrast, exposure of normal fibroblasts to gamma-rays resulted in a rapid up-regulation of p53 and the level peaked at 2 h post-irradiation. XP cells with a severe deficiency in the nucleotide excision repair pathway showed abnormally high levels of p53 protein in response to 4NQO treatment, indicating that lesions other than incision-associated DNA strand breaks trigger p53 up-regulation. We observed a consistent, inverse correlation between the ability of the various fibroblast cultures to induce p53 following 4NQO treatment and their DNA repair efficiencies. Treatment with 0.12 microM 4NQO, for example, caused a >2-fold up-regulation of p53 in excision repair-deficient (AT, XPA and XPG) strains without eliciting any effect on p53 levels in repair-proficient (normal and XPE) strains. We conclude that up-regulation of p53 by 4NQO is mediated solely by an ATM-independent mechanism and that the p53 response is primarily triggered by persistent alkali-stable 4NQO-DNA adducts.  (+info)

Development of a new bioluminescent mutagenicity assay based on the Ames test. (4/242)

A newly developed rapid mutagenicity assay based on the adenosine triphosphate (ATP)-bioluminescence technique and the Ames test is described. Salmonella typhimurium strains TA98 and TA100 were exposed in an appropriate liquid medium to the direct mutagens 4-nitroquinoline-N-oxide and methyl methanesulphonate, respectively, and to the indirect mutagen 2-aminoanthracene. Both auxotrophic and prototrophic growth were monitored throughout the incubation period as variations in the intracellular ATP levels by means of the luciferin-luciferase assay. After 9-12 h of incubation a dose-response increase in the levels of ATP was readily detected. In order to demonstrate that this increase was due to the growth of revertant bacteria, aliquots from each culture were plated on minimal agar plates. A very good correlation between the changes in ATP levels and the appearance of revertant colonies on the plates was found. Given the rapidity of this method as compared with conventional mutagenicity assays, it has potential for industrial and environmental applications. Other potential applications are also discussed.  (+info)

Enzymatic and DNA binding properties of purified WRN protein: high affinity binding to single-stranded DNA but not to DNA damage induced by 4NQO. (5/242)

Mutations in the WRN gene result in Werner syndrome, an autosomal recessive disease in which many characteristics of aging are accelerated. A probable role in some aspect of DNA metabolism is suggested by the primary sequence of the WRN gene product. A recombinant His-tagged WRN protein (WRNp) was overproduced in insect cells using the baculovirus system and purified to near homogeneity by several chromatographic steps. This purification scheme removes both nuclease and topoisomerase contaminants that persist following a single Ni(2+)affinity chromatography step and allows for unambiguous interpretation of WRNp enzymatic activities on DNA substrates. Purified WRNp has DNA-dependent ATPase and helicase activities consistent with its homology to the RecQ subfamily of proteins. The protein also binds with higher affinity to single-stranded DNA than to double-stranded DNA. However, WRNp has no higher affinity for various types of DNA damage, including adducts formed during 4NQO treatment, than for undamaged DNA. Our results confirm that WRNp has a role in DNA metabolism, although this role does not appear to be the specific recognition of damage in DNA.  (+info)

Regulation of the ribonucleotide reductase small subunit gene by DNA-damaging agents in Dictyostelium discoideum. (6/242)

In Escherichia coli, yeast and mammalian cells, the genes encoding ribonucleotide reductase, an essential enzyme for de novo DNA synthesis, are up-regulated in response to DNA damaging agents. We have examined the response of the rnrB gene, encoding the small subunit of ribonucleotide reductase in Dictyostelium discoideum, to DNA damaging agents. We show here that the accumulation of rnrB transcript is increased in response to methyl methane sulfonate, 4-nitroquinoline-1-oxide and irradiation with UV-light, but not to the ribonucleotide reductase inhibitor hydroxyurea. This response is rapid, transient and independent of protein synthesis. Moreover, cells from different developmental stages are able to respond to the drug in a similar fashion, regardless of the basal level of expression of the rnrB gene. We have defined the cis -acting elements of the rnrB promoter required for the response to methyl methane sulfonate and 4-nitroquinoline-1-oxide by deletion analysis. Our results indicate that there is one element, named box C, that can confer response to both drugs. Two other boxes, box A and box D, specifically conferred response to methyl methane sulfonate and 4-nitroquinoline-1-oxide, respectively.  (+info)

Rsp5 ubiquitin-protein ligase mediates DNA damage-induced degradation of the large subunit of RNA polymerase II in Saccharomyces cerevisiae. (7/242)

Rsp5 is an E3 ubiquitin-protein ligase of Saccharomyces cerevisiae that belongs to the hect domain family of E3 proteins. We have previously shown that Rsp5 binds and ubiquitinates the largest subunit of RNA polymerase II, Rpb1, in vitro. We show here that Rpb1 ubiquitination and degradation are induced in vivo by UV irradiation and by the UV-mimetic compound 4-nitroquinoline-1-oxide (4-NQO) and that a functional RSP5 gene product is required for this effect. The 26S proteasome is also required; a mutation of SEN3/RPN2 (sen3-1), which encodes an essential regulatory subunit of the 26S proteasome, partially blocks 4-NQO-induced degradation of Rpb1. These results suggest that Rsp5-mediated ubiquitination and degradation of Rpb1 are components of the response to DNA damage. A human WW domain-containing hect (WW-hect) E3 protein closely related to Rsp5, Rpf1/hNedd4, also binds and ubiquitinates both yeast and human Rpb1 in vitro, suggesting that Rpf1 and/or another WW-hect E3 protein mediates UV-induced degradation of the large subunit of polymerase II in human cells.  (+info)

The ATPase domain but not the acidic region of Cockayne syndrome group B gene product is essential for DNA repair. (8/242)

Cockayne syndrome (CS) is a human genetic disorder characterized by UV sensitivity, developmental abnormalities, and premature aging. Two of the genes involved, CSA and CSB, are required for transcription-coupled repair (TCR), a subpathway of nucleotide excision repair that removes certain lesions rapidly and efficiently from the transcribed strand of active genes. CS proteins have also been implicated in the recovery of transcription after certain types of DNA damage such as those lesions induced by UV light. In this study, site-directed mutations have been introduced to the human CSB gene to investigate the functional significance of the conserved ATPase domain and of a highly acidic region of the protein. The CSB mutant alleles were tested for genetic complementation of UV-sensitive phenotypes in the human CS-B homologue of hamster UV61. In addition, the CSB mutant alleles were tested for their ability to complement the sensitivity of UV61 cells to the carcinogen 4-nitroquinoline-1-oxide (4-NQO), which introduces bulky DNA adducts repaired by global genome repair. Point mutation of a highly conserved glutamic acid residue in ATPase motif II abolished the ability of CSB protein to complement the UV-sensitive phenotypes of survival, RNA synthesis recovery, and gene-specific repair. These data indicate that the integrity of the ATPase domain is critical for CSB function in vivo. Likewise, the CSB ATPase point mutant failed to confer cellular resistance to 4-NQO, suggesting that ATP hydrolysis is required for CSB function in a TCR-independent pathway. On the contrary, a large deletion of the acidic region of CSB protein did not impair the genetic function in the processing of either UV- or 4-NQO-induced DNA damage. Thus the acidic region of CSB is likely to be dispensable for DNA repair, whereas the ATPase domain is essential for CSB function in both TCR-dependent and -independent pathways.  (+info)

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Bothwell KD, Shaurova T, Merzianu M, Suresh A, Kuriakose MA, Johnson CS, Hershberger PA, Seshadri M. Impact of short-term 1,25-dihydroxyvitamin D3 on the chemopreventive efficacy of erlotinib against oral cancer. Cancer Prev Res (Phila). June 22015. Publication Date June 2015 How Analyze was Used Following MR image acquisition, raw image sets were transferred to a… ...
The purpose of this study was to recover DNA sequence information from the HIS4A region of chromosome III in a large number of independently arising, 4-nitroquinoline N-oxide (4NQO)-induced His$\sp+$ revertants of the +1 frameshift mutation his4-38 in Saccharomyces cerevisiae strains XY729 (REV1) and XY760 (rev1-1). These two strains differ in their ability to repair DNA. The REV1 gene is involved in error-prone repair of DNA. The two strains differed in their sensitivity to the lethal effects of 4NQO. To control for this difference both strains were treated with a concentration of 4NQO that led to 37% survival (one lethal hit). To obtain the DNA sequence information, methods for DNA amplification by the polymerase chain reaction (PCR) and subsequent dideoxy sequencing of the double-stranded PCR product were developed. PCR was optimized to generate a high yield of the amplified DNA, and to increase the stringency of the reaction to prevent the formation of artifacts. Double-stranded, dideoxy ...
TY - JOUR. T1 - Mutation spectrum of 4-nitroquinoline 1-oxide-damaged single-stranded shuttle vector DNA transfected into monkey cells. AU - Fronza, Gilberto. AU - Madzak, Catherine. AU - Campomenosi, Paola. AU - Inga, Alberto. AU - Iannone, Raffaella. AU - Abbondandolo, Angelo. AU - Sarasin, Alain. PY - 1994/7/16. Y1 - 1994/7/16. N2 - 4-Nitroquinoline 1-oxide (4NQO) is a potent mutagen and carcinogen which induces two main guanine adducts at positions C8 and N2. We recently determined the mutation spectrum induced by the ultimate metabolite of 4NQO, acetoxy-4-aminoquinoline 1-oxide in the M13lacZ′/E. coli lacZΔM15 α-complementation assay. Our data suggested that dGuo-C8-AQO induces (per se or via AP sites) G to Pyr transversions. Here we report our study on 4NQO mutagenesis in monkey cells. 4NQO lesions were induced in vitro on a single-stranded (ss) DNA shuttle vector carrying the supF tRNA gene. This vector was able to replicate both in mammalian cells and in bacteria. The mutations ...
TY - JOUR. T1 - RAD9-dependent G1 arrest defines a second checkpoint for damaged DNA in the cell cycle of Saccharomyces cerevisiae. AU - Siede, Wolfram. AU - Friedberg, Andrew S.. AU - Friedberg, Errol C.. PY - 1993/9/1. Y1 - 1993/9/1. N2 - Exposure of the yeast Saccharomyces cerevisiae to ultraviolet (UV) light, the UV-mimetic chemical 4-nitroquinoline-1-oxide (4NQO), or γ radiation after release from G1 arrest induced by α factor results in delayed resumption of the cell cycle. As is the case with G2 arrest following ionizing radiation damage [Weinert, T. A. & Hartwell, L. H. (1988) Science 241, 317-322], the normal execution of DNA damage-induced G1 arrest depends on a functional yeast RAD9 gene. We suggest that the RAD9 gene product may interact with cellular components common to the G1/S and G2/M transition points in the cell cycle of this yeast. These observations define a checkpoint in the eukaryotic cell cycle that may facilitate the repair of lesions that are otherwise processed to ...
The promoter of nrdA gene which is related with DNA synthesis was used to construct a DNA damage sensitive biosensor. A recombinant bioluminescent E. coli strain, BBTNrdA, harboring a plasmid with the nrdA promoter fused to the luxCDABE operon, was successfully constructed. Its response to various chemicals including genotoxic chemicals substantiates it as a DNA damage biosensor. In characterization, three different classes of toxicants were used: DNA damaging chemicals, oxidative stress chemicals, and phenolics. BBTNrdA only responded strongly to DNA damaging chemicals, such as nalidixic acid (NDA), mitomycin C (MMC), 1-methyl-1-nitroso-N-methylguanidine (MNNG), and 4-nitroquinoline N-oxide (4-NQO). In contrast, there were no responses from the oxidative stress chemicals and phenolics, except from hydrogen peroxide (H2O2) which is known to cause DNA damage indirectly. Therefore, the results of the study demonstrate that BBTNrdA can be used as a DNA damage biosensor.
The promoter of nrdA gene which is related with DNA synthesis was used to construct a DNA damage sensitive biosensor. A recombinant bioluminescent E. coli strain, BBTNrdA, harboring a plasmid with the nrdA promoter fused to the luxCDABE operon, was successfully constructed. Its response to various chemicals including genotoxic chemicals substantiates it as a DNA damage biosensor. In characterization, three different classes of toxicants were used: DNA damaging chemicals, oxidative stress chemicals, and phenolics. BBTNrdA only responded strongly to DNA damaging chemicals, such as nalidixic acid (NDA), mitomycin C (MMC), 1-methyl-1-nitroso-N-methylguanidine (MNNG), and 4-nitroquinoline N-oxide (4-NQO). In contrast, there were no responses from the oxidative stress chemicals and phenolics, except from hydrogen peroxide (H2O2) which is known to cause DNA damage indirectly. Therefore, the results of the study demonstrate that BBTNrdA can be used as a DNA damage biosensor.
In this expanded study, we confirmed a previously reported association between in vitro BPDE-induced mutagen sensitivity and the risk of SCCHN ( 9). Because Dr. T. C. Hsu developed the mutagen sensitivity assay with bleomycin as an effective inducer of chromosomal aberrations in vitro, this assay has been widely used in epidemiologic studies to assess genetic susceptibility to various types of cancer. The assay has been modified with a variety of etiologically related challenge mutagens to evaluate cancer susceptibility in association studies, such as BPDE for tobacco-related cancers of the lung ( 4, 6- 8) and SCCHN ( 9- 11), γ-radiation for glioma ( 22, 23), and UV light or 4-nitroquinoline-1-oxide for skin cancer ( 24, 25). These studies have consistently showed that in vitro induced mutagen sensitivity phenotype is a risk factor for developing cancers.. Because the above-mentioned studies used a case control study design, it is questionable whether mutagen sensitivity is a tumor marker or a ...
Novel and effective targets for cancer therapy and prevention are increasingly important in cancer research, especially for head and neck squamous cell carcinoma (HNSCC), which is one of the most lethal cancers. A potential target for this type of cancer might be sphingosine kinase 1 (SphK1) which phosphorylates sphingosine to form sphingosine-1-phosphate (S1P) because it is a critical regulator of sphingolipid-mediated functions and the SphK1/S1P pathway has been implicated in several important biological functions including inflammation and carcinogenesis. To investigate the potential of SphK1 as a target in head and neck cancer development, we used a 4-nitroquinoline-1-oxide (4-NQO)-induced carcinogenesis tongue model in SphK1 knockout mice. All mice were treated with 4-NQO at 40 μg/ml in drinking water for 14 weeks (untreated water was available thereafter, until the end of the experiment). All animals were sacrificed at 20 weeks and animal tongues were examined. We found that SphK1 ...
3-Nitroquinoline 1-oxide | C9H6N2O3 | CID 81935 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
n-Amyl bromide was tested in an in vitro cytogenetics assay using duplicate cultures of CHO cells. Treatments covering a broad range of doses, separated by narrow intervals, were performed. The test article was dissolved in dimethyl sulphoxide and the highest dose level used was 500 µg/ml. Treatment was performed in the absence of S-9 for 20 hours proir to harvest (20 +0). The test article dose levels for chromosome analysis were selected by evaluating the effect of n-Amyl bromide on cell number. Chromosome aberrations were analysed at three consecutive dose levels. The highest concentration chosen for analysis, 163.8 µg/ml, induced approximately 74 ù reduction in cell number and 58 % mitotic inhibition. Appropriate negative (solvent) control cultures were included in the test system. The proportion of cells with structural aberrations in these cultures fell within the historical solvent control range. 4 -Nitroquinoline-1-oxide was employed as the positive control chemical and cells receiving ...
Animal models for carcinogenesis of the oral cavity are limited, although this disease is often fatal or disfiguring and its incidence in the USA is ~30 000 cases/year. Short-term whole-animal models for this disease should prove valuable in the investigation of factors affecting oral carcinogenesis. In this study we observed that a group of oral carcinogens are clearly mutagenic in the lacZ transgenic mouse oral cavity. The carcinogens 4-nitroquinoline-N-oxide (4-NQO), benzo[a]pyrene (B[a]P), N-nitroso-N-methylurea (NMU), 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), nitrosonornicotine (NNN) and 7,12-dimethylbenzanthracene (DMBA) were all mutagenic in a mixture of pooled oral tissues (gingival, buccal, pharyngeal and sublingual) and in the tongue. All agents except DMBA (which was swabbed in the oral cavity) and B[a]P (by gavage) were given in drinking water for 2-4 weeks followed by a 2 week expression period before killing. With one exception, groups of 4-5 female mice were treated. ...
Asian Pac J Cancer Prev. 2013;14(11):6991-5.. Antioxidant and anti-cancer cell proliferation activity of propolis extracts from two extraction methods.. Khacha-ananda S1, Tragoolpua K, Chantawannakul P, Tragoolpua Y.. Author information. Abstract. Antioxidant activity, total phenolic, total flavonoid compounds and cytotoxicity to cancer cell lines of propolis extracts from two extraction methods were investigated in this study. Propolis was collected from Phayao province and extracted with 70% ethanol using maceration and sonication techniques. The antioxidant activity was evaluated by DPPH assay. Total phenolic and flavonoid compounds were also determined. Moreover, the cytotoxicity of propolis was evaluated using MTT assay. The percentage propolis yield after extraction using maceration (18.1%) was higher than using sonication (15.7%). Nevertheless, antioxidant and flavonoid compounds of the sonication propolis extract were significant greater than using maceration. Propolis extract from ...
TY - JOUR. T1 - RAD3 gene of Saccharomyces cerevisiae. T2 - Nucleotide sequence of wild-type and mutant alleles, transcript mapping, and aspects of gene regulation. AU - Naumovski, L.. AU - Chu, G.. AU - Berg, P.. AU - Friedberg, E. C.. PY - 1985. Y1 - 1985. N2 - We determined the complete nucleotide sequence of the RAD3 gene of S. cerevisiae. The coding region of the gene contained 2,334 base pairs that could encode a protein with a calculated molecular weight of 89,796. Analysis of RAD3 mRNA by Northern blots and by S1 nuclease mapping indicated that the transcript was approximately 2.5 kilobases and did not contain intervening sequences. Fusions between the RAD3 gene and the lacZ gene of Escherichia coli were constructed and used to demonstrate that the RAD3 gene was not inducible by DNA damage caused by UV radiation or 4-nitroquinoline-1-oxide. Two UV-sensitive chromosomal mutant alleles of RAD3, rad3-1 and rad3-2, were rescued by gap repair of a centromeric plasmid, and their sequences ...
Could be an ATP-dependent permease. Confers hyper-resistance to the mutagens 4-nitroquinoline-N-oxide (4-NQO) and triaziquone, as well as to the chemicals sulphomethuron methyl phenanthroline when present in multiple copies. Exhibits nucleoside triphosphatase activity.
TY - JOUR. T1 - Correlation between Musashi-1 and c-hairy-1 expression and cell proliferation activity in the developing intestine and stomach of both chicken and mouse. AU - Asai, Rieko. AU - Okano, Hideyuki. AU - Yasugi, Sadao. N1 - Copyright: Copyright 2008 Elsevier B.V., All rights reserved.. PY - 2005/10. Y1 - 2005/10. N2 - Musashi-1 (Msi-1) is an RNA-binding protein that plays key roles in the maintenance of neural stem cell states and in their differentiation into neural cells. Msi-1 has also been proposed as a candidate marker gene of mammalian intestinal stem cells and their immediate lineages. In this study, we examined Msi-1 expression in the small intestine and the stomach of both chicken and mouse during embryonic, fetal and postnatal development. In addition, we analyzed the expression of c-hairy-1, a chicken homologue of mouse Hes1, and assessed the proliferative activity of the cells expressing both of these factors. Significantly, during the development of these digestive organs ...
Wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Extinguishing media: Use agent most appropriate to extinguish fire ...
RECOMMENDED: If you have Windows errors then we strongly recommend that you download and run this (Windows) Repair Tool.. Jun 12, 2017. Office VBA Reference Language Reference VBA Class doesnt support Automation (Error 430). Class doesnt support Automation (Error 430).. Microsoft Access 2010 Error Numbers and Descriptions - Microsoft Access 2010 comprehensive list all Error Numbers and Descriptions. how to read/write/update/delete record in tables using vba. - Nov 06, 2008 · how to read/write/update/delete record in tables using vba. Discussion in Microsoft Access VBA Modules started by mike g, Nov 6, 2008.. Post subject: Solution to VBA error 430 Class does not support automation: I got this error when I tried to use a macro which had run great on other machines.. Nov 11, 2008. When I try to create a new object of a class module from this dll I get a Run timer error 430: Class does not support automation or does not.. Problem names and reserved words in Access. This list, from the Database ...
So, I am a complete beginner to VBA. Yet, I have been trying to figure out the code for this using various internet sources. So my idea for this is that i have two sheets of data (say sheet1 and sheet2). Sheet1 is my master list and sheet2 has all the…
I am trying to sort columns based on their rank.(Code, 1 line)However, I look for the column number each rank - I keep getting an error. I am new at VBA. When I copy and paste the values in the cells and then do the find it works. Not sure what to do.…
The VBA Programming Language specification defines the implementation-independent and operating-system-independent programming language that must be supported by all conforming VBA implementations.
Affordable learning tools in advanced Excel VBA modeling in finance, statistics, and mathematics through our VBA source code tutorials.
I have asked this question in another site, but got no replies, not to speak of a solution. How do I generate an arglist with Excel 97 / VBA ? The code below for an array formula is an example...
Dear Experts, I would like to know which is the row number of the first blank cell in column A, could you please advise how it should be in VBA? thanks,
Hello All, I need help with some VBA. I have written a macro which utilises Autofilters, but it seems to fail when autofilter is already turned on (ev
Excel 97 Win98 How can I format the entire range named Cal ? The range is now is B3:H75 I want the first line the default format (bold and font.size=
A polysaccharide capsule is one of the most important virulence factors for the pathogenic fungus Cryptococcus neoformans. We previously characterized two capsule-associated genes,CAP59 and CAP64. To further dissect the molecular mechanism of capsule synthesis, 16 acapsular mutants induced by 4-nitroquinoline-1-oxide were obtained. The acapsular phenotype of one of these mutants was complemented. The cloned gene was designatedCAP60, and deletion of this newly described capsule-associated gene resulted in an acapsular phenotype. The proposed 67-kDa Cap60p contains 592 amino acids and appears to have a putative transmembrane domain close to the N terminus. DNA sequence analysis revealed that CAP60 has similarity toCAP59 at the center portion of its coding regions. Contour-clamped homogeneous electric field blot analysis suggested that these two genes are on the same chromosome. CAP60 andCAP59, however, could not be functionally substituted for each other by direct complementation or by domain swap ...
Guanazole and aphidicolin were chosen as candidates in the search for a selective, non-genotoxic inhibitor of DNA replication which could be used instead of hydroxyurea to measure DNA repair synthesis in rat hepatocyte primary cultures by liquid scintillation counting. The genotoxicity of these 3 chemicals was studied using the Salmonella/liver homogenate assay and the autoradiographic UDS test in hepatocytes. Hydroxyurea was positive in both of these assays. Guanazole and aphidicolin did not induce DNA repair in hepatocytes. Aphidicolin was not mutagenic for Salmonella typhimurium, whereas guanazole increased the revertant numbers of strain TA102 slightly. The incorporation of [3H]thymidine was measured by liquid scintillation to determine DNA repair induced by 2-acetylaminofluorene (2-AAF), aflatoxin B1, benzo[a]pyrene, cyclophosphamide, H2O2, 6-hydroxydopamine, N-methyl-N-nitro-N-nitrosoguanidine (MNNG), methylnitrosourea (MNU), 4-nitroquinoline-N-oxide and UV irradiation in the presence of ...
Applications are invited for 2 full-time postdoctoral positions in tumor/immuno-biology to study the implication of extracellular matrix (ECM) components in the immunosuppressive reprogramming of the tumor microenvironment in head and neck cancer associated with tobacco use. The project aims to provide a better understanding of the cellular, molecular and functional interactions between tumor-associated innate immune cells and ECM molecules during carcinogenesis of head and neck squamous epithelium. Studies will be conducted using an immunocompetent mouse model of tongue carcinogenesis and ex vivo 3D reconstituted ECM systems. Implications of our findings for human cancer and therapeutic intervention will be determined by analyzing samples from human patients. This collaborative project will provide training in an interdisciplinary context at the crossroads of immunology, cell and molecular biology and human tumor pathology.. One position is available in the Institute of Biology Valrose (iBV) ...
The clastogenic potential of Subtilisin, Batch No. PPA 6865 was evaluated by its effect on chromosomes of human peripheral blood lymphocytes according to OECD guideline 473 (July 1997). Subtilisin is a proteolytic enzyme which implies, that the test substance has the potential of breaking down the metabolising system S9. Therefore, the main study was conducted with heat-inactivated test substance. Heparinized whole blood cultures from three male donors were established, and division of the lymphocytes was stimulated by adding phytohaemagglutinin to the cultures. Two independent experiments were performed both in the absence and presence of metabolic activation by a rat S-9 mix induced with Aroclor. Sets of duplicate cultures were treated with the solvent (sterile purified water), test substance or positive controls (-S-9: 4-Nitroquinoline 1-oxide, +S-9: Cyclophosphamide). Treatments with subtilisin covered a broad range of doses, where the highest dose level used was 5000 ug/mL. In the first ...
I have written a Rule Engine in MS Access (client) that uses linked tables with SQL Server Native Client to SQL Server 2008 R2. There are around 150 Binary Rules. Each Binary Rule has combinations of SQL and other logic. They are designed to return a True/False. These Binary Rules are the first tier for the Rule Engine conversion. The process is now to take the tested VBA and convert each Binary Rule to TSQL UDF. The next layer, a Status Rule - will take one parameter and then call on dozens of the Binary Rules. Depending on the status number, it will expect different Binary Rules to return some pattern of True or False. [b]The code below is the most simple rule. The purpose is to get a template for conversion from VBA to TSQL. [/b] [code=vb] Function Rule71(ID_Wells As Integer) As Boolean Rule 71 Well is a Facility - Pass in Primary Key for Well, run SQL for this ID. If record returns then True/ else False Dim rstMisc As DAO.Recordset Dim
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If you are used to using VBA, you can do as follow:. 1. Select a cell of the column you want to select and press Alt + F11 to open the Microsoft Visual Basic for Applications window.. 2. In the pop-up window, click Insert , Module, then paste the following VBA code into the module.. VBA: Shrink text to fit cell ...
Range Dimensioning, Range and Value Referencing and Referring to Arrays Hi, . I have been learning VBA for about 6months now but still get a bit bogged down on getting to grips with understanding some basic Range assigning and Definition. I need help from someone that understands the core as it were,. of how the computer or VBA actually works.. . This post and the specific questions here follows on from a question that came up coincidentally yesterday here Post #10 - # 14
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Tongue squamous cell carcinoma (TSCC) is one of the most common types of oral squamous cell carcinomas (OSCC). TSCC is highly invasive and
Mutation plot summary of 60 oral tongue squamous cell carcinoma patients showing frequently mutated genes. The top plot shows the key clinical parameters, below
Background: Oral tongue squamous cell carcinoma (OTSCC) causes over 350,000 cases annually and particularly impacts populations in developing countries....
4NQO reliably produced preneoplastic and malignant oral cavity lesions, which morphologically and histologically mimic human head and neck cancer. Lesions develop long after 4NQO exposure and without an inflammatory response. Thus, the model should be useful for molecular analysis of neoplastic tran …
A mononuclear Ni(ii) complex [Ni(NCS)2(nqu)2(H2O)2]·2nqu (nqu - 5-nitroquinoline) shows a field induced slow magnetic relaxation with three relaxation domains. The relaxation time for the low-frequency mode is as slow as τ = 0.3 s at T = 1.9 K and BDC = 0.4 T.
Cal 27 was established in 1982 by J. Gioanni (Centre Antoine Lacassagne, Nice Cedex, France) from tissue taken prior to treatment from a 56 year old Caucasian male with a lesion of the middle of the tongue.
Video created by University of Colorado Boulder for the course Excel/VBA for Creative Problem Solving, Part 1. Week 3 teaches you all about how to reference and move information to VBA from Excel and vice versa. Youll learn about the various ...
Before I forget, Im typing this out! We had a successful 2VBA2C!!! My little guy was born at 8:10 Monday morning after a hard foug...
Get Excel VBA to count cells by color. Input range. Use this to also perform color by sum, sumbycolor or countbycolor tasks. Its all about sum by color.
Project VBA: How and Why it can Make You a Project Guru!. Rod Gill Founder ACE Project Systems Ltd. A Project Guru is not just a power user Project Gurus are great at managing and reporting on, project information Slideshow 92670 by ivanbritt
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Thats a crappy UI. And thats from someone who spends a lot of time in the Visual Basic Editor.. Then its settled. Ill build my own form for changing the properties I want to change. Its what I really wanted to do anyway, so why stop lying to myself. What kind of features should I build into this UI? A big textbox is a must. Also, Id like to be able to add white space and line breaks. Oh, and if I could have SQL parsing, autoformatting, and autocomplete… So basically what I want is SQL Server Management Studio. I already have that. Its called SQL Server Management Studio. That lead me to my next bit of genius. If I want to edit the SQL, even only a little, I should do it in SSMS. I added a couple of buttons to the Ribbon.. ...
First, I am reluctant to download your workbook because it contains macros. I dont know you and I cant be sure that you havent included malicious code in the workbook. If you want us to look at the code, please copy and paste it into this forum....
Microsoft MVP for Project 1997-2016. Professional scheduler, project manager and advanced trainer of MS Project. I also develop VBA macros for Project and Excel to provide automated reporting for projects and programs of projects ...
When youre ready to start editing and testing VBA code on your own, set up the VB Editor to work for you. Here are some tips for customizing the editor.
81 (4): 862-878. doi:10.1111/j.1749-6632.1959.tb49372.x. PMID 14440840. S2CID 29982301. David, Gottlieb; Paul Dale, Shaw (1967 ... 100 (4): 1547-1551. Bibcode:2003PNAS..100.1547C. doi:10.1073/pnas.0337625100. PMC 149869. PMID 12556563. Type strain of ... ISBN 0-412-46620-1.CS1 maint: numeric names: authors list (link) Liu, editors-in-chief, Lewis Mander, Hung-Wen (2010). ... ISBN 978-3-211-99323-1. Grote, R; Zeeck, A; Drautz, H; Zähner, H (September 1988). "Metabolic products of microorganisms. 244. ...
44 (1): 45-51. doi:10.1006/geno.1997.4838. PMID 9286699. Hisama FM, Chen YH, Meyn MS, et al. (2000). "WRN or telomerase ... 10 (1): 13-22. doi:10.1111/j.1365-2443.2004.00812.x. PMID 15670210. S2CID 24977510. Rual JF, Venkatesan K, Hao T, et al. (2005 ... 1493 (1-2): 231-6. doi:10.1016/s0167-4781(00)00153-6. PMID 10978529. Szekely AM, Chen YH, Zhang C, et al. (2000). "Werner ... 29 (1): 179-86. doi:10.1006/geno.1995.1229. PMID 8530069. "Entrez Gene: POLD2 polymerase (DNA directed), delta 2, regulatory ...
The molecular formula C9H6N2O3 (molar mass: 190.16 g/mol, exact mass: 190.0378 u) may refer to: 4-Nitroquinoline 1-oxide ...
... polyvinylpyridine n-oxide MeSH D03.383.725.762 - pyridinium compounds MeSH D03.383.725.762.232 - cetylpyridinium MeSH D03.383. ... 4,5-tetrahydro-8-chloro-3-methyl-5-phenyl-1h-3-benzazepin-7-ol MeSH D03.438.079.800 - 2,3,4,5-tetrahydro-7,8-dihydroxy-1-phenyl ... 4,5-dihydro-1-(3-(trifluoromethyl)phenyl)-1h-pyrazol-3-amine MeSH D03.383.129.539.200 - epirizole MeSH D03.383.129.539.487 - ... 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, methyl ester MeSH D03.383.725.210 - dimethindene MeSH D03.383. ...
... polyvinylpyridine n-oxide MeSH D02.455.326.271.884.533.699 - povidone MeSH D02.455.326.271.884.533.710 - povidone-iodine MeSH ... ethylene oxide MeSH D02.355.291.411.900 - trichloroepoxypropane MeSH D02.355.291.705 - okadaic acid MeSH D02.355.291.852 - ... vitamin k 1 MeSH D02.455.849.291.523.500.844 - vitamin k 2 MeSH D02.455.849.291.523.500.922 - vitamin k 3 MeSH D02.455.849.291. ... 4,4'-(3-oxo-1,5-pentanediyl)bis(n,n-dimethyl-n-2-propenyl-), dibromide MeSH D02.092.146.325 - p-dimethylaminoazobenzene MeSH ...
IL-1 - IL-1-alfa - IL-11 - IL-12 - IL-2 - IL-3 - IL-4 - IL-6 - ileostomy - iloprost - ILX-295501 - ILX23-7553 - IM-862 - ... HTLV-1 - hu14.18-interleukin-2 fusion protein - Huang Lian - human epidermal growth factor receptor 2 - human leukocyte antigen ... B7-1 - Bacillus Calmette Guérin - bacterial toxin - barium enema - barium solution - barium swallow - Barrett's esophagus - ... RSV S-1 - S-phase fraction - safingol - salpingo-oophorectomy - salvage therapy - samarium 153 - saponin - saquinavir mesylate ...
In enzymology, a nitroquinoline-N-oxide reductase (EC 1.7.1.9) is an enzyme that catalyzes the chemical reaction 4-( ... quinoline N-oxide:NADP+ oxidoreductase. Other names in common use include 4-nitroquinoline 1-oxide reductase, 4NQO reductase, ... hydroxyamino)quinoline N-oxide + 2 NAD(P)+ + H2O ⇌ {\displaystyle \rightleftharpoons } 4-nitroquinoline N-oxide + 2 NAD(P)H + 2 ... On the reducing enzyme of 4-nitroquinoline-N-oxide]". Nichidai Igaku Zasshi. 24: 423-432. Stanley JS, York JL, Benson AM (1992 ...
... (also known as 4-NQO, 4NQO, 4Nqo, NQO and NQNO) is a quinoline derivative and a tumorigenic compound ... 28 (4): 409-21. doi:10.1101/gad.228940.113. PMC 3937518. PMID 24532717. Fry, Rebecca C.; Begley, Thomas J.; Samson, Leona D. ( ... 4-nitroquinoline 1-oxide (4NQO) is a quinoline, a carcinogenic and mutagenic chemical. Quinolines, like 4NQO, possess a ... 4 (9): 1169-73. doi:10.1093/carcin/4.9.1169. PMID 6883639. Ikenaga, Mituo; Ichikawa-Ryo, Haruko; Kondo, Sohei (1975). "The ...
ISBN 0-525-24210-4. CS1 maint: discouraged parameter (link) Karnow, Stanley (1997). Vietnam: A history. London: Penguin Books. ... ISBN 0-670-84218-4. CS1 maint: discouraged parameter (link) Mantienne, Frédéric (October 2003). "The Transfer of Western ... These involved the fundamental ring systems and derivatives of 1,2-benzanthracene, the dibenzopyrenes, steranthrenes, ... anthanthrene, 1,2,3,4-dibenzanthracene, a variety of benzo and dibenzofluoranthenes. This was extended to large-molecular-size ...
Quinoline is nitrated to 3-nitroquinoline and 7-nitroquinoline. Pyridine is nitrated to 3-nitropyridine and 4-nitropyridine. " ... Nienow, Amanda M.; Jeffrey T. Roberts (2006). "Chemical Vapor Deposition of Zirconium Oxide on Aerosolized Silicon ... ZrCl4 + 4 N2O5 → Zr(NO3)4 + 4ClNO2 The product can be purified by sublimation in a vacuum. A contaminating substance in this is ... 54 (1): 93-98. doi:10.1007/s11172-005-0222-7. ISSN 1066-5285. Wah Chang (10 September 2003). "Zirconium in Nitric Acid ...
... nitroquinoline-N-oxide reductase EC 1.7.1.10: hydroxylamine reductase (NADH) EC 1.7.1.11: 4-(dimethylamino)phenylazoxybenzene ... S-oxide reductase EC 1.8.4.13: L-methionine (S)-S-oxide reductase EC 1.8.4.14: L-methionine (R)-S-oxide reductase EC 1.8.5.1: ... trimethylamine-N-oxide reductase (cytochrome c) EC 1.7.2.4: nitrous-oxide reductase EC 1.7.2.5: nitric oxide reductase ( ... nitrous-oxide reductase EC 1.7.99.7: nitric-oxide reductase EC 1.7.99.8: hydroxylamine oxidoreductase EC 1.8.1.1: deleted EC ...
4-Nitroquinoline 1-oxide (also known as 4-NQO, 4NQO, 4Nqo, NQO and NQNO) is a quinoline derivative and a tumorigenic compound ... 28 (4): 409-21. doi:10.1101/gad.228940.113. PMC 3937518. PMID 24532717. Fry, Rebecca C.; Begley, Thomas J.; Samson, Leona D. ( ... 4-nitroquinoline 1-oxide (4NQO) is a quinoline, a carcinogenic and mutagenic chemical. Quinolines, like 4NQO, possess a ... 4 (9): 1169-73. doi:10.1093/carcin/4.9.1169. PMID 6883639. Ikenaga, Mituo; Ichikawa-Ryo, Haruko; Kondo, Sohei (1975). "The ...
5-Chloro-4-nitroquinoline 1-oxide , C9H5ClN2O3 , CID 84188 - structure, chemical names, physical and chemical properties, ...
2002 Jun 27;518(1):85-93. Research Support, U.S. Govt, P.H.S. ... Guttenplan JB1, Kosinska W, von Pressentin Md, Rosa J, El- ... 4-NQO was a potent mutagen in tongue, other pooled oral tissues and esophagus when given in drinking water for 4 weeks at a ... 1. Division of Basic Sciences/Biochemistry, New York University, Dental Center, 345 E. 24th St., New York, NY 10100, USA. ... Effects of 1,4-phenylenebis(methylene)selenocyanate (p-XSC) and vitamin E on 4-nitroquinoline-N-oxide (4-NQO)-induced ...
Bothwell KD, Shaurova T, Merzianu M, Suresh A, Kuriakose MA, Johnson CS, Hershberger PA, Seshadri M. Impact of short-term 1,25- ... Impact of short-term 1,25-dihydroxyvitamin D3 on the chemopreventive efficacy of erlotinib against oral cancer ...
1 This investigation was supported in part by Grants CA 13747, CA 19283, and CA 15901 awarded by the National Cancer Institute ... Interactions of the Carcinogen 4-Nitroquinoline 1-oxide with the Non-Protein Thiols of Mammalian Cells. Marie E. Varnes and ... The carcinogen 4-nitroquinoline 1-oxide (4-NQO) was found to rapidly deplete non-protein thiols (NPSH) from Ehrlich ascites ... The NPSH thus appeared to play a significant role in 4-NQO detoxification. Glutathione, when present in culture medium during 4 ...
1 This work was supported in part by Grant 05671568 from the Ministry of Health and Welfare of Japan, a Grant-in-Aid for ... Starting 1 week after the stop of 4-NQO exposure, the groups given 4-NQO and a basal diet were switched to the diets containing ... Seven days after the 4-NQO exposure, groups of animals fed the diets containing test chemicals were switched to the basal diet ... Chemoprevention of 4-Nitroquinoline 1-Oxide-induced Oral Carcinogenesis by Dietary Curcumin and Hesperidin: Comparison with the ...
Of these 86 of the XY729 revertants and 94 of the XY760 revertants were due to $-$1 frameshift events, with the rest of the ... The mutation spectra of 4-nitroquinoline N-oxide-induced frameshift reversion ofhis4-38 in REV1 and rev1-1 strains of ... The mutation spectra of 4-nitroquinoline N-oxide-induced frameshift reversion ofhis4-38 in REV1 and rev1-1 strains of ... The mutation spectra of 4-nitroquinoline N-oxide-induced frameshift reversion ofhis4-38 in REV1 and rev1-1 strains of ...
XP cells are not only abnormally sensitive to UV, but also to a variety of chemical carcinogens, including 4-nitro-quinoline-1- ... oxide (4NQO). Complementation analysis with XP strains from 4 different complementation groups with respect to the repair of ... Gene- and strand-specific damage and repair in Chinese hamster ovary cells treated with 4-nitroquinoline 1-oxide.. Elizabeth G ... XP cells are not only abnormally sensitive to UV, but also to a variety of chemical carcinogens, including 4-nitro-quinoline-1- ...
Survivin and inducible nitric oxide synthase production during 4NQO-induced rat tongue carcinogenesis: A possible relationship ... Ras gene mutation is not related to tumour invasion during rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide  ... The role of matrix metalloproteinases 2 and 9 during rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide  ... Imbalance of Tumor Suppression Genes Expression Following Rat Tongue Carcinogenesis Induced by 4-Nitroquinoline 1-Oxide  ...
4-NQO. A substance that is used in cancer research to cause tumors in laboratory animals. This is done to test new diets, drugs ... OXIDE - оксид - acid oxide - aluminium oxide - calcium oxide - carbonic oxide - chrome oxide - copper oxide - cupric oxide - ... OXIDE - n. calcium oxide deuterium oxide nitric oxide nitrous oxide oxide mineral. Britannica Concise Encyclopedia ... OXIDE - окись; оксид - calcium oxide - lead oxide - oxide of aluminium - zinc oxide. Англо-Русский словарь по строительству и ...
... ... Chemopreventive activity of apple extract following medium-term oral carcinogenesis assay induced by 4-nitroquinoline-1-oxide. ... Group 4, received apple extract at 1% by gavage for 8 consecutive weeks only; and Group 5, received 4NQO for 8 weeks in ... Group 1, negative control group (non-treated group); Group 2, received 4NQO during 8 weeks in drinking water and treated with ...
Chemopreventive activity of systemically administered curcumin on oral cancer in the 4-nitroquinoline 1-oxide model. en. ... 4-NQO). A total of 50 male albino rats, Rattus norvegicus, (Holtzman), were divided into five groups (n=10 per group). Four of ... these groups were exposed to 50 ppm 4-NQO in their drinking water ad libitum for 8 or 12 weeks, two groups were treated with ... study was to evaluate the chemopreventive activity of systemically administered curcumin on oral carcinogenesis induced by 4- ...
4 Methods for Investigating Addictive Potential 149-190 * 5 Methods for Studying Risk Perception and Risk Communication 191-220 ... B Chapters 1 and 2 from Evaluation of Biomarkers and Surrogate Endpoints in Chronic Disease ... Appendix B: Chapters 1 and 2 from *Evaluation of Biomarkers andSurrogate Endpoints in Chronic Disease* 257-258 ... 1-1 Diseases and Conditions Caused by Active Cigarette Smoking. 1-2 Summary of Milestones in Decreasing Indoor Tobacco Smoke in ...
They were then fed a diet containing 0.01% or 0.05% fenofibrate for 29 weeks, starting 1 week after 4-NQO administration. At ... The incidence and multiplicity of lesions were significantly lower in mice treated with 4-NQO and 0.05% fenofibrate compared ... Our results indicate that fenofibrate can prevent the development of 4-NQO-induced proliferative lesions in the lung by ... This study was designed to determine whether fenofibrate, a PPARα agonist, can suppress 4-nitroquinoline 1-oxide (4-NQO)- ...
4ニトロキノリンまたはコバルト60ガンマ線で不死化したヒト線維芽細胞において減少するタンパク質の二次元電気泳動による解析 ... analysis of down-regulated proteins in human fibroblasts immortalized by treatment with either 4-nitroquinoline 1-oxide or 60Co ... analysis of down-regulated proteins in
In vitro DNA modification by the ultimate carcinogen of 4-nitroquinoline-1-oxide : Influence of superhelicity. / Menichini, P. ... In vitro DNA modification by the ultimate carcinogen of 4-nitroquinoline-1-oxide: Influence of superhelicity. Carcinogenesis. ... title = "In vitro DNA modification by the ultimate carcinogen of 4-nitroquinoline-1-oxide: Influence of superhelicity", ... In vitro DNA modification by the ultimate carcinogen of 4-nitroquinoline-1-oxide: Influence of superhelicity. ...
4-Nitroquinoline 1-oxide (4NQO) is a potent mutagen and carcinogen which induces two main guanine adducts at positions C8 and ... N2 - 4-Nitroquinoline 1-oxide (4NQO) is a potent mutagen and carcinogen which induces two main guanine adducts at positions C8 ... AB - 4-Nitroquinoline 1-oxide (4NQO) is a potent mutagen and carcinogen which induces two main guanine adducts at positions C8 ... Mutation spectrum of 4-nitroquinoline 1-oxide-damaged single-stranded shuttle vector DNA transfected into monkey cells. In: ...
inducible Nitric Oxide Synthase. Keap1. Kelch-like erythroid cell-derived protein with Capncollar homology-associated protein ... Shafquat Azim 1, Aamir Ahmad 1. , Mohammad Aslam Khan 1, Girijesh Kumar Patel 1, Seema Singh 1,2 and Ajay Pratap Singh 1,2,* ... zinc-oxide and titanium-dioxide nanoparticles against uvb-induced DNA damage for the prevention of skin carcinogenesis. Cancer ... up-regulation and down-regulation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX2) [30], as well as by ...
4-NQO). The cell proliferation activity measured by proliferating cell nuclear antigen (PCNA)-positive index and apop … ... 2 selective inhibitor nimesulide on tongue carcinogenesis were investigated in male F344 rats initiated with 4-nitroquinoline-1 ... and inducible nitric oxide synthase (iNOS) in the tongue mucosa or neoplasms were also examined for mechanistic analysis of ... 2003 Jan;18(1):39-48. doi: 10.14670/HH-18.39. Authors K Yoshida 1 , T Tanaka, H Kohno, K Sakata, T Kawamori, H Mori, K ...
1) Treatment of cell cultures:. - at least 10E7 cells in a volume of 16 mL of RPMI 5 were placed in a series of sterile ... Reference 1. Endpoint:. in vitro gene mutation study in bacteria. Type of information:. experimental study. Adequacy of study: ... 1 hour after test article removal, Cyto-B (formulated in DMSO) was added to post wash-off culture medium to give a final ... Experiment 1:. - the following materials were mixed in a test tube and poured onto the selective agar plates: 100 μL test ...
Both substances are UVCB substances, containing metallic oxides; each substance is obtained as a by-product in the manufacture ... Both substances are UVCB substances, containing metallic oxides; each substance is obtained as a by-product in the manufacture ... Both substances are UVCB substances, containing metallic oxides; each substance is obtained as a by-product in the manufacture ... Table 4: Spontaneous Mutation Rates (Concurrent Negative Controls), Experiment 2 Number of Revertants (mean number of colonies ...
Reference 4. Endpoint:. in vitro DNA damage and/or repair study. Type of information:. experimental study. Adequacy of study:. ... 1 µg/plate for TA100, 2 µg/plate for TA1535 and TA1537, 10 µg/plate for WP2uvrA; with S9-mix. Untreated negative controls:. yes ... Mutation assay 1:. 5.38, 10.8, 21.5, 43.0, 86.0, 172 µg/mL (with and without S9; 3h treatment). Mutation assay 2:. 5.38, 10.8, ... Main experiment 1, with S9, 15 µg/mL and 23 µg/mL, dissolved in sterile water. Untreated negative controls:. yes. Negative ...
These data are edited from Figure 1 of Ref. [48] for BY4741, Δerg6, 12geneΔ0, and 12geneΔ0HSR, or Figure 2 of Ref. [49] for ... The work flow is shown in Figure 1. As a first step, we focused on drug efflux systems. The drug efflux system composed of ABC ... 2.2.1. Availability of 12geneΔ0HSR-iERG6 in drug screening. In general, S. cerevisiae exhibits high levels of drug resistance, ... Figure 1.. The work flow of the construction of multidrug-sensitive strains. (A) The parental strain, BY4741, possesses high ...
Reference 1. Endpoint:. in vitro gene mutation study in bacteria. Remarks:. Type of genotoxicity: gene mutation. Type of ... other: 4-nitroquinoline-1-oxide, benzo(a)pyrene. Details on test system and experimental conditions:. METHOD OF APPLICATION: in ... other: 4-Nitroquinoline 1-oxide. Details on test system and experimental conditions:. METHOD OF APPLICATION: in medium; in ... 1) the proportions of cells with structural aberrations at one or more concentration exceeds the normal range in both ...
In enzymology, a nitroquinoline-N-oxide reductase (EC 1.7.1.9) is an enzyme that catalyzes the chemical reaction 4-( ... quinoline N-oxide:NADP+ oxidoreductase. Other names in common use include 4-nitroquinoline 1-oxide reductase, 4NQO reductase, ... hydroxyamino)quinoline N-oxide + 2 NAD(P)+ + H2O ⇌ {\displaystyle \rightleftharpoons } 4-nitroquinoline N-oxide + 2 NAD(P)H + 2 ... On the reducing enzyme of 4-nitroquinoline-N-oxide]". Nichidai Igaku Zasshi. 24: 423-432. Stanley JS, York JL, Benson AM (1992 ...
Kondo T1, Mihara K, Inoue Y, Namba M.. Author information. 1. Department of Cell Biology, Okayama University Medical School, ... we established three immortalized human fibroblast cell lines by treatment with either 4-nitroquinoline 1-oxide (4NQO) or 60Co ... on down-regulated intracellular transferrin in human fibroblasts immortalized by treatment with either 4-nitroquinoline 1-oxide ...
Ethers, oxides, and epoxides Diepoxybutane p-Dioxane 4-Nitroquinoline-1-oxide. Hydrazines 1,1-Dimethylhydrazine 1,2- ... pentoxide A Mcalcium oxide C ҥH n P V X ɥ N o ǤƾǪ [ J ????A H?? K E P ?? ӿE q C ... 1. r ʤƾǪ G r ʤƾǪ G k ʤƾǪ (corrosive chemical) P ֽ IJ ɡA , ϥֽ ?? ݱo } a γy ä[ ??? A Ϊֽ̥ , ] ?? Ǥ?? Ө ˡC q ` b ??? Ǥ o ǻG k ʪ ... 1) l J G q ` o Ǫ , y I l Y i f ˮ` A ~ A ] , ͪ B ͤ ~ B I l D H } a H Ψt Ωʤ r C Ҧp G ??, y I l D Y
Taichiro Nonaka,1,2 Yoshinobu Toda,3 Hiroshi Hiai,4 Munehiro Uemura,1 Motonobu Nakamura,5 Norio Yamamoto,6 Ryo Asato,6 Yukari ... Figure 4. Molecular characterization of spontaneous SCC in K14-AID-Tg mice. (A) Codon distribution of Hras1 somatic mutations. ... The top 4 codon numbers of mutations in human skin SCC are shown. (D) Base substitution patterns and frequency of the ... 25 and Supplemental Tables 3 and 4). The AID mutation signature is considered a C-to-T transition preceded by G or A (26), ...
Accepted name: nitroquinoline-N-oxide reductase. Reaction: 4-(hydroxyamino)quinoline N-oxide + 2 NAD(P)+ + H2O = 4- ... Accepted name: nitric oxide reductase (cytochrome c). Reaction: nitrous oxide + 2 ferricytochrome c + H2O = 2 nitric oxide + 2 ... EC 1.7.2.3 trimethylamine-N-oxide reductase (cytochrome c). EC 1.7.2.4 nitrous-oxide reductase. EC 1.7.2.5 nitric oxide ... Accepted name: nitric oxide reductase (menaquinol). Reaction: 2 nitric oxide + menaquinol = nitrous oxide + menaquinone + H2O. ...
For construction of individual mutant strains in the RDKY3615 (MATa, ura3-52, leu2Δ1, trp1Δ63, his3Δ200, lys2ΔBgl, hom3-10, ... The hom3-10 assay measures the reversion of a +1 insertion in the HOM3 gene (7). The lys2Δ-Bgl assay measures the reversion of ... Mutation of ELG1 causes sensitivity to both methyl methanesulfonate and 4-nitroquinoline 1-oxide (50). ELG1 mutations result in ... Table 1. Rank order of the 33 BY4741 Canr mutator strains ... ade2Δ1, ade8, hxt13::URA3) background (10), which is highly ...
  • 4-Nitroquinoline 1-oxide (also known as 4-NQO, 4NQO, 4Nqo, NQO and NQNO) is a quinoline derivative and a tumorigenic compound used in the assessment of the efficacy of diets, drugs, and procedures in the prevention and treatment of cancer in animal models. (wikipedia.org)
  • 4-nitroquinoline 1-oxide (4NQO) is a quinoline, a carcinogenic and mutagenic chemical. (wikipedia.org)
  • Both 4NQO and its reduced metabolite 4-hydroxyaminoquinoline 1-oxide (4HAQO) bind covalently to cellular macromolecules such as nucleic acids and proteins. (wikipedia.org)
  • 4NQO's four electron reduction product, 4-hydroxyaminoquinoline 1-oxide (4HAQO), is believed to be a carcinogenic metabolite of 4NQO. (wikipedia.org)
  • The purpose of this study was to recover DNA sequence information from the HIS4A region of chromosome III in a large number of independently arising, 4-nitroquinoline N-oxide (4NQO)-induced His$\sp+$ revertants of the +1 frameshift mutation his4-38 in Saccharomyces cerevisiae strains XY729 (REV1) and XY760 (rev1-1). (illinois.edu)
  • XP cells are not only abnormally sensitive to UV, but also to a variety of chemical carcinogens, including 4-nitro-quinoline-1-oxide (4NQO). (semanticscholar.org)
  • 4-Nitroquinoline 1-oxide (4NQO) is a potent mutagen and carcinogen which induces two main guanine adducts at positions C8 and N2. (elsevier.com)
  • We recently determined the mutation spectrum induced by the ultimate metabolite of 4NQO, acetoxy-4-aminoquinoline 1-oxide in the M13lacZ′/E. coli lacZΔM15 α-complementation assay. (elsevier.com)
  • Other names in common use include 4-nitroquinoline 1-oxide reductase, 4NQO reductase, and NAD(P)H2:4-nitroquinoline-N-oxide oxidoreductase. (wikipedia.org)
  • To investigate the mechanisms of immortalization, we established three immortalized human fibroblast cell lines by treatment with either 4-nitroquinoline 1-oxide (4NQO) or 60Co gama rays. (nih.gov)
  • Keratinocyte-specific Tid1-deficient mice with 4-nitroquinoline-1-oxide (4NQO) treatment exhibited increased protein levels of Galectin-7 and had a poor survival rate. (thno.org)
  • The carcinogenic 4-nitroquinoline-1-oxide (4NQO) model has proven to be successful although until now it is unknown at what time point the established tumor is a representative squamous cell carcinoma and has a suitable volume for scientific treatment. (eur.nl)
  • Herein, we investigated the potential chemopreventive activity of sulforaphane using in vitro models of normal and malignant mucosal epithelial cells and an in vivo model of murine oral cancer resulting from the carcinogen 4-nitroquinoline-1-oxide (4NQO). (aacrjournals.org)
  • Escherichia coli resistance to helix-distorting mutagens, such as ultraviolet (UV) radiation, 4-nitroquinoline-1-oxide (4NQO), and nitrofurazone (NFZ), depends on the intracellular level of ppGpp ( 8 , 10 ) ( Fig. 1A and fig. S1). (sciencemag.org)
  • Aphidicolin (APC) and the combination of cytosine-1-β-D-arabinofuranoside and hydroxyurea (araC/HU) enhanced comet-tail formation by UV, a UV-mimetic agent, 4-nitroquinoline-1-oxide (4NQO), and methylating agents, methyl methanesulfonate (MMS) and methyl nitrosourea (MNU). (go.jp)
  • TEX were delivered (IV) to immunocompetent C57BL/6 mice bearing pre-malignant oral/esophageal lesions induced by the carcinogen, 4-Nitroquinoline 1-oxide (4NQO). (izon.com)
  • The aim of this study was to evaluate the chemopreventive potential of purple carrot extract following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide (4NQO). (readbyqxmd.com)
  • Glutathione, when present in culture medium during 4-NQO treatment, protected V79 cells from 4-NQO toxicity. (aacrjournals.org)
  • Unfortunately, these strategies have also not provided substantial improvement due to the development of resistance against therapies [ 3 ], reviving an interest in the prospects of phytochemicals, natural anticancer agents from plants, due to the multitude of effects of these agents on diverse molecular signaling pathways, with no or minimal toxicity in normal cells [ 4 ]. (mdpi.com)
  • Toxicity parameters of 4-nitroquinoline-1-oxide, urethane and methyl methanesulfonate in the flare and Oregon- flare strains of Drosophila melanogaster . (ou.edu)
  • These drugs have an advantage over the synthetic drugs in terms of low or no toxicity at the effective dose [ 4 ]. (hindawi.com)
  • In enzymology, a nitroquinoline-N-oxide reductase (EC 1.7.1.9) is an enzyme that catalyzes the chemical reaction 4-(hydroxyamino)quinoline N-oxide + 2 NAD(P)+ + H2O ⇌ {\displaystyle \rightleftharpoons } 4-nitroquinoline N-oxide + 2 NAD(P)H + 2 H+ The 4 substrates of this enzyme are 4-hydroxyaminoquinoline N-oxide, NAD+, NADP+, and H2O, whereas its 4 products are 4-nitroquinoline N-oxide, NADH, NADPH, and H+. (wikipedia.org)
  • The systematic name of this enzyme class is 4-(hydroxyamino)quinoline N-oxide:NADP+ oxidoreductase. (wikipedia.org)
  • T. Kakunaga, Caffeine inhibits cell transformation by 4-nitro-quinoline-1-oxide. (springer.com)
  • Study on expression of LIM domain only protein 1 in SD rat oral buccal mucosa carcinogenesis induced by 4-nitro-quinoline N-oxide]. (physiciansweekly.com)
  • The carcinogen 4-nitroquinoline 1-oxide (4-NQO) was found to rapidly deplete non-protein thiols (NPSH) from Ehrlich ascites tumor cells and V79 Chinese hamster fibroblasts. (aacrjournals.org)
  • Sulforaphane treatment of Het-1A, a normal mucosal epithelial cell line, and 4 HNSCC cell lines led to dose- and time-dependent induction of NRF2 and the NRF2 target genes NQO1 and GCLC , known mediators of carcinogen detoxication. (aacrjournals.org)
  • The cell proliferation activity measured by proliferating cell nuclear antigen (PCNA)-positive index and apoptotic index, and the immunohistochemical expression of COX-2, and inducible nitric oxide synthase (iNOS) in the tongue mucosa or neoplasms were also examined for mechanistic analysis of modifying effects of nimesulide on tongue carcinogenesis. (nih.gov)
  • Results suggest GPE may be useful for prevention and treatment of hypertension through increasing NO (nitric oxide) production in blood vessels. (stuartxchange.com)
  • The modifying effects of two natural products, curcumin and hesperidin, given during the initiation and postinitiation phases of oral carcinogenesis initiated with 4-nitroquinoline 1-oxide (4-NQO) were investigated in male F344 rats and compared with that of β-carotene. (aacrjournals.org)
  • The modifying effects of a cyclooxygenase (cox)-2 selective inhibitor nimesulide on tongue carcinogenesis were investigated in male F344 rats initiated with 4-nitroquinoline-1-oxide (4-NQO). (nih.gov)
  • All animals except those treated with nimesulide alone and untreated rats were given 20 ppm 4-NQO in drinking water for 8 weeks to induce tongue neoplasms. (nih.gov)
  • Starting 1 week after the cessation of 4-NQO exposure, rats given 4-NQO were fed the experimental diets containing nimesulide (100 and 400 ppm) for 22 weeks. (nih.gov)
  • Here, we investigated the modifying effects of dietary feeding with a naturally occurring polyphenolic antioxidant flavonoid silymarin on 3,2′-dimethyl-4-aminobiphenyl (DMAB)-induced prostatic carcinogenesis in male F344 rats. (aacrjournals.org)
  • Oral administration of a diet containing 20% betel-nut powder enhanced the incidences of preneoplastic and neoplastic lesions of the tongue in rats pretreated with 4-nitroquinoline-1-oxide and of preneoplastic liver lesions in rats pretreated with 2-acetylaminofluorene [ref: 2]. (inchem.org)
  • Aqueous extracts of betel leaf were tested in mice by oral intubation and by intraperitoneal injection, in hamsters by application to the cheek-pouch mucosa [ref: 1] and in rats by oral administration [ref: 3]. (inchem.org)
  • Journal Article] An animal model for the rapid induction of tongue neoplasms in human c-Ha-ras proto-oncogene transgenic rats by 4-nitroquinoline 1-oxide : its potential use for preclinical chemoprevention studies. (nii.ac.jp)
  • Generically, carcinogenic effects of chemicals in bladder carcinogenesis are judged by induction of papillary or nodular (PN) hyperplasia in rats given N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) for 4 weeks and the test chemical for 22-28 weeks. (biomedsearch.com)
  • Genetic controls of susceptibility and resistance to 4-nitroquinoline 1-oxide-induced tongue carcinomas in rats. (mysciencework.com)
  • We analyzed the incidence of infiltrative mass-type tongue carcinomas (IMTC) induced in 550 rats by continuous oral administration of 0.001% 4-nitroquinoline 1-oxide solution for 180 days. (mysciencework.com)
  • The goal of this study was to evaluate the chemopreventive activity of systemically administered curcumin on oral carcinogenesis induced by 4-nitroquinolone-1-oxide (4-NQO). (unesp.br)
  • These findings demonstrate that the systemic administration of curcumin has chemopreventive activity during oral carcinogenesis induced by 4-NQO. (unesp.br)
  • Sakaki, T., Tamura, I., Kadota, H. and Kakudo, K. (2003) Changing Expression of E- and P-Cadherin during Rat Tongue Carcinogenesis Induced by 4-Nitroquinoline 1-Oxide. (scirp.org)
  • 1 This investigation was supported in part by Grants CA 13747, CA 19283, and CA 15901 awarded by the National Cancer Institute, NIH. (aacrjournals.org)
  • At 7 weeks of age, all animals except those treated with each test chemical alone and control groups were given 4-NQO (20 ppm) in the drinking water for 8 weeks to induce oral cancer. (aacrjournals.org)
  • 1 This work was supported in part by Grant 05671568 from the Ministry of Health and Welfare of Japan, a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan, and a 1993 grant from the Sagawa Foundation for Promotion of Cancer Research in Japan. (aacrjournals.org)
  • It is estimated that in 2017, the United States alone will have 1,688,780 new cancer diagnoses and 600,920 cancer-related deaths [ 1 ]. (mdpi.com)
  • To date, the cancer problem and the failure of conventional chemotherapy to achieve a reduction in the mortality rates for common epithelial malignancies such as carcinomas of the lung, colon, breast, prostate and pancreas, indicates a critical need for new approaches to control cancer development [ 1 , 2 ]. (mdpi.com)
  • Inherited or acquired deficiencies in genome maintenance systems contribute significantly to the onset of cancer as evidenced by the observation that a number of the DNA-repair and checkpoint genes are mutated in cancer susceptibility syndromes and sporadic cancers ( 1 - 4 ). (pnas.org)
  • For the most current information, contact the National Cancer Institute via the Internet web site at http://cancer.gov or call 1-800-4-CANCER. (healthlinkbc.ca)
  • Prostate cancer is the most common type of cancer found in older men and the leading cause of cancer mortality in men ( 1 ). (aacrjournals.org)
  • Furthermore, migrant studies have shown that the incidence of prostate cancer increases generation by generation after immigration in Japanese-Americans ( 4 ). (aacrjournals.org)
  • In another case-control study of oral cancer, in which a clear effect of chewing betel with tobacco was found, no such effect was found for chewing betel without tobacco [ref: 1]. (inchem.org)
  • It is known that mutations in somatic cells play a key role in cancer initiation and carcinogenesis process [ 1 ]. (hindawi.com)
  • Cancer is a major health problem in the developed world, just after aging-related cardiovascular disease, with 14 million cases worldwide and 8.2 million of deceases just in 2012 1 . (frontiersin.org)
  • Moreover, epidemiological studies indicate that many types of cancer are dependent on multiple mutational etiologies, as well as on inherited mutator phenotype [ 4 , 10 - 15 ]. (intechopen.com)
  • We therefore investigated the expression of plectin-1 and trichohyalin in human tongue epithelial cell line (DOK) and tongue cancer cell line (BICR31) using western blotting and FITC-labeled immunocytochemistry techniques. (scirp.org)
  • OPLs vary histologically from hyperkeratosis with hyperplasia to severe dysplasia or carcinoma in situ and progress to squamous cell carcinoma in a substantial percentage of cases and there is no proven approach for reducing the oral cancer risk of OPLs ( 1 ). (aacrjournals.org)
  • in a rat model of tongue carcinogenesis, nimesulide (a selective COX-2 inhibitor) decreased the incidence of 4-nitroquinoline-1-oxide-induced cancer ( 25 ). (aacrjournals.org)
  • It is widely recognized that oxidation of proteins play an essential role in pathogenesis of cancer [ 4 ] Indeed knockout of various antioxidant defense enzymes promotes cancer development in animals. (alliedacademies.org)
  • Since hypoalbuminemia is one of the most common features of cancer, this study aims at relating it with oxidative stress [ 1 ]. (alliedacademies.org)
  • 1 Notwithstanding the global declining incidence of gastric cancer, mortality is still rising in Asian countries. (bmj.com)
  • Fairness of skin, UV sensitivity and skin cancer risk all correlate with the physiologic function of the melanocortin 1 receptor, a G s -coupled signaling protein found on the surface of melanocytes. (jove.com)
  • Oral cancer is considered to be a preventable condition, due to the possibility of early detection and treatment ( 1 ). (spandidos-publications.com)
  • Snuff and alcohol consumption are associated with 90% of patients that exhibit oral cancer ( 1 ) and the two factors appear to have a synergistic effect ( 4 ). (spandidos-publications.com)
  • Worldwide, approximately 600,000 new cases of head and neck cancer HNC are diagnosed each year, and oral cancer comprises about half of these cases and is considered the most representative site of this tumor type [1]. (thefreelibrary.com)
  • Literature data also show a high prevalence of oral cancer, which has been attributed to oral sex leading to increased infection with human papilloma virus (HPV) [3, 4]. (thefreelibrary.com)
  • carcinomas occurred in the cheek pouch following implantation of wax pellets [ref: 1]. (inchem.org)
  • In hamsters, applications of such extracts to cheek-pouch mucosa produced squamous-cell carcinomas of the cheek pouch and forestomach carcinomas [ref: 1]. (inchem.org)
  • In hamsters, administration of areca nut and application of aqueous or dimethyl sulphoxide extracts to the cheek-pouch mucosa resulted in squamous-cell carcinomas of the cheek pouch and carcinomas of the forestomach [ref: 1]. (inchem.org)
  • We found that deletion of CAP1 did not affect the susceptibility of CJD21 cells to FCZ, cerulenin, brefeldin A, and diamide but caused hypersensitivity to cadmium, 4-nitroquinoline N -oxide, 1,10-phenanthroline, and hydrogen peroxide, an effect which was reverted by reintroduction of the CAP1 gene in these cells. (asm.org)
  • Wilkey JF, Buchberger G, Saucier K, et al: Cyclin D1 overexpression increases susceptibility to 4-nitroquinoline-1 -oxide-induced dysplasia and neoplasia in murine squamous oral epithelium. (spandidos-publications.com)
  • Genetic controls of susceptibility and resistance to 4-nitroqu. (mysciencework.com)
  • These results suggest that 400 ppm nimesulide in diet, when given during the promotion phase, exerts chemopreventive ability against 4-NQO-induced tongue tumorigenesis through inhibition of cell proliferation activity in conjunction with modification of COX-2 and iNOS expression of the target lesions. (nih.gov)
  • Major sources of DNA lesions are physical and chemical agents from the environment, intermediates of cellular metabolism, spontaneous chemical reactions of DNA, incorporation of foreign or damaged nucleotides, etc. [ 1 , 2 ]. (intechopen.com)
  • We report the case of a 51-year-old female Caucasian patient, with no history of smoking, who presented white lesions on the tongue and an ulcerated lesion on the lower lip beginning 4 months prior. (bvsalud.org)
  • These results indicated that natural compounds curcumin and hesperidin inhibited rat oral carcinogenesis initiated with 4-NQO (relatively weak in hesperidin) as did β-carotene, and such inhibition might be related to suppression of cell proliferation. (aacrjournals.org)
  • Inhibition of 4-nitroquinoline-1-oxide-induced oral carcinogenesis by dietary calcium. (semanticscholar.org)
  • As model substances, 4-nitroquinoline-1-oxide (NQO), hydrogen peroxide and phorbol 12-myristate 13-acetate (PMA) were selected. (uni-wuerzburg.de)
  • The drug 4 -nitroquinoline 1-oxide ( 4 NQO) displays both carcinogenic and antitumor effects, a well known characteristic of many chemotherapeutic drugs. (umsystem.edu)
  • 1 The resulting thymine -thymine cyclobutane dimer product was soon identified as the major DNA lesion formed by UV irradiation of cells and was associated with the mutagenic and carcinogenic effects of solar irradiation. (rsc.org)
  • Similarly, YafP increased cytotoxicity of two DNA damaging nitroaromatic compounds, 4-nitroquinoline-1-oxide (NQO) and nitrofurazone, in the absence of PolIV. (nih.gov)
  • 5. Berks, B.C., Ferguson, S.J., Moir, J.W. and Richardson, D.J. Enzymes and associated electron transport systems that catalyse the respiratory reduction of nitrogen oxides and oxyanions. (qmul.ac.uk)
  • 4 , 11 The protein content includes 22 essential amino acids 2 , 12 and the total protein is nutritionally superior to legume protein, but inferior to meat protein. (drugs.com)
  • Werner patient mutations truncate the WRN open reading frame and promote loss of the altered protein and both of its associated biochemical activities ( 4 , 28 , 42 ). (asm.org)
  • This gene encodes a protein highly homologous to the S. cerevisiae bZip transcription factor Yap1p and was named CAP1 , for C. albicans AP-1. (asm.org)
  • Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. (abcam.com)
  • Recent studies indicate that increased production of reactive oxygen species (ROS) can promote development of malignancy .Over production of ROS within the tissue can damage DNA, proteins, lipids and carbohydrates [ 1 , 2 ], producing oxidized products like advanced oxidation protein products (AOPP), malondialdehyde (MDA),advanced glycation end products etc [ 3 ]. (alliedacademies.org)
  • AZ may itself be regulated by the binding of a protein AzI ( Figure 1 ), which binds to antizyme more tightly than ODC and thus releases ODC from the AZ-ODC complex [ 2 , 6 , 11 , 12 ]. (portlandpress.com)
  • P2X7R mRNA and protein were present in unstimulated C6 cells and were up-regulated by cell exposure to the P2X7R agonist, 2',3'-(benzoyl-4-benzoyl)-ATP (BzATP). (biomedsearch.com)
  • Two-dimensional electrophoretic studies on down-regulated intracellular transferrin in human fibroblasts immortalized by treatment with either 4-ni. (nih.gov)
  • DA showed a 93.6% incidence of IMTC measuring more than 5 mm in their largest diameter, while WF showed only a 4% incidence. (mysciencework.com)
  • To further dissect the molecular mechanism of capsule synthesis, 16 acapsular mutants induced by 4-nitroquinoline-1-oxide were obtained. (asm.org)
  • No epistatic interactions were found, either for the spontaneous mutator phenotype or for MMS, EMS, and 4-NQO sensitivity. (genetics.org)
  • BBTNrdA only responded strongly to DNA damaging chemicals, such as nalidixic acid (NDA), mitomycin C (MMC), 1-methyl-1-nitroso-N-methylguanidine (MNNG), and 4-nitroquinoline N-oxide (4-NQO). (mdpi.com)
  • The newly developed method was used to evaluate four different mutagenesis systems: a new ARTP tool, ultraviolet radiation, 4-nitroquinoline-1-oxide (4-NQO), and N -methyl- N' -nitro- N -nitrosoguanidine (MNNG) mutagenesis. (springer.com)
  • 4-NQO was a potent mutagen in tongue, other pooled oral tissues and esophagus when given in drinking water for 4 weeks at a concentration of 20 microg/ml [corrected]. (nih.gov)
  • These results indicate that trichohyalin is expressed by cancerous tongue epithelial cells during various stages of malignancy and that plectin-1 provides an index of malignancy. (scirp.org)
  • Phospholipase C gamma 1 is a potential prognostic biomarker for patients with locally advanced and resectable oral squamous cell carcinoma. (semanticscholar.org)
  • Head and neck squamous cell carcinoma (HNSCC) is one of the most common cancers worldwide with fewer than half of HNSCC patients surviving beyond five years [ 1 ]. (thno.org)
  • Both V79 and Ehrlich cells contained appreciable amounts of glutathione S -transferase (EC 2.5.1.18), which catalyzes the nucleophilic substitution of the nitro group of 4-NQO with thiols. (aacrjournals.org)
  • In addition, reaction of thiols with nitro radicals or with nitrosoquinoline 1-oxide was indicated by the inhibitory effect of glutathione on oxygen consumption in solutions of 4-NQO and sodium ascorbate. (aacrjournals.org)
  • Repair of DNA damage after exposure to 4-nitroquinoline-1-oxide in heterokaryons derived from xeroderma pigmentosum cells. (semanticscholar.org)
  • Among these plants, it has been reported that antioxidants, a mixture of components that can reduce reactive oxygen species (ROS), induce mutagens or improve alkylated DNA damage [ 3 , 4 ]. (hindawi.com)
  • In addition, it has also been shown to modify the mutagenic response of ionizing radiation, UV, and several chemical mutagens in a number of test systems.We have studied the effect of caffeine on gamma radiation and 4-Nitroquinoline 1-oxide (4-NQO)-induced gene conversion in the yeast Saccharomyces cerevisiae D7. (begellhouse.com)
  • Seven days after the 4-NQO exposure, groups of animals fed the diets containing test chemicals were switched to the basal diet and continued on this diet until the end of the study. (aacrjournals.org)
  • Starting 1 week after the stop of 4-NQO exposure, the groups given 4-NQO and a basal diet were switched to the diets containing β-carotene, hesperidin, and curcumin and maintained on these diets for 22 weeks. (aacrjournals.org)
  • It is a reduction product of 4-NITROQUINOLINE-1-OXIDE. (nih.gov)
  • By-product of the manufacture of ferromanganese alloy containing primarily oxides of aluminum, manganese and silicon. (europa.eu)
  • While the incision of UV-induced pyrimidine dimers creates a long gap of about 30 bp during nucleotide excision repair (NER) that is re-synthesized and repaired by DNA polymerase δ/ε, base excision repair (BER) creates a short gap of 1-6 bp that is closed by DNA polymerase β. (go.jp)
  • The effects of NPSH on 4-NQO metabolism were studied by measuring 4-hydroxyaminoquinoline 1-oxide formation, CN −1 -insensitive oxygen consumption, and reduction of ferricytochromes c + c 1 in normal cells and in cells pretreated with the thiol reagent N -ethylmaleimide. (aacrjournals.org)
  • True stationary phase sets in weeks after glucose is exhausted and cells shift from fermentation to oxidative carbon metabolism and is characterized by specific gene expression ( 4 ). (asm.org)
  • The present study was performed as part of an attempt to authenticate the use of Inula racemosa root extract as traditional medicine in India by experimentally investigating their protective effects on 4-nitroquinoline-1-oxide (4-NQO) induced DNA damage and apoptosis in mice bone marrow cells. (hindawi.com)
  • 4-NQO (7.5 mg/kg bw) was injected intraperitoneally to the mice on the sixth day. (hindawi.com)
  • This was the first report that recorded the protective effects of I. racemosa on 4-NQO-induced DNA damage and apoptosis in mice bone marrow cells. (hindawi.com)
  • Journal Article] Suppressive effect of 1,4-phenylene diisothiocyanate on N-butyl N-(4-hydroxybutyl)nitrosamine-induced urinary bladder carcinogenesis in male ICR mice. (nii.ac.jp)
  • This review summarizes recent work on the regulation of putrescine production both de novo and via the degradation of higher polyamines and provides a summary of studies using transgenic mice in which the levels of proteins that regulate these processes ( L -ornithine decarboxylase, antizyme and spermidine/spermine- N 1 -acetyltransferase) are altered. (portlandpress.com)
  • Reasoning that eumelanin deposition might be enhanced by topical agents that mimic Mc1r signaling, we found that direct application of forskolin extract to the skin of Mc1r-defective fair-skinned mice resulted in robust eumelanin induction and UV protection 1 . (jove.com)
  • In mice with 4-nitroquinoline 1-oxide-induced tumorigenesis, 21-day oral treatment with APW (1600 mg/kg) decreased the level of dysplasia in esophagus and significantly modulated the population of regulatory T cells. (greenmedinfo.com)
  • prostaglandins are important in the postinitiation phases of tumorigenesis through modulation of immune surveillance ( 3 ), stimulation of cell proliferation, and angiogenesis ( 4 ). (aacrjournals.org)
  • Effects of 1,4-phenylenebis(methylene)selenocyanate (p-XSC) and vitamin E on 4-nitroquinoline-N-oxide (4-NQO)-induced mutagenesis in lacZ mouse upp. (nih.gov)
  • Gene- and strand-specific damage and repair in Chinese hamster ovary cells treated with 4-nitroquinoline 1-oxide. (semanticscholar.org)
  • We find that, at the restrictive temperature, the rad4-/cut5- cells fail to delay mitosis in response to gamma-irradiation whilst retaining a normal checkpoint response to the UV-mimetic drug 4-nitroquinoline-1-oxide. (nih.gov)
  • Experimental data have suggested that C-phycocyanin can selectively inhibit release of histamine from mast cells and prevent increases in immunoglobulin E. A small study in patients with mild to moderate asthma suggested that spirulina supplementation (1 g/day) produced improvement in lung function parameters. (drugs.com)
  • T. Aida, and W.J. Bodell, Caffeine potentiates cytotoxicity and sister chromatid exchange induction in resistant rat brain tumor cells treated with 1,3-bis (2-chloroethyl)-l-nitrosourea. (springer.com)
  • More recently, silymarin has been reported to inhibit activation of erbB1 signaling, induce cyclin-dependent kinase inhibitors, G 1 arrest, and cause complete inhibition of growth of human prostate carcinoma DU145 cells ( 17 ). (aacrjournals.org)
  • These mechanisms are largely conserved among prokaryotes and eukaryotes, including human cells [ 3 , 4 ]. (intechopen.com)
  • ABSTRACT: In basal squamous cells, plectin-1 interacts with intermediate filaments, whereas trichohyalin, which is distributed primarily in the medulla and inner root sheath cells of human hair follicles, plays a role in strengthening cells during keratinization. (scirp.org)
  • DOK and BICR31 cells were cultivated to subconfluence in Dulbecco's Modified Eagle's Medium containing 0.4 μg/ml of hydrocortisone and 10% fetal bovine serum, and the levels of trichohyalin and plectin-1 were determined by western blot analysis and immunocytochemical staining. (scirp.org)
  • Although DOK cells expressed trace levels of plectin-1, obvious plectin-1 bands were detected in western blot analyses of BICR31 cells. (scirp.org)
  • Trichohyalin was diffusely distributed in both cell lines, and colocalization of trichohyalin and cytokeratin 1/10 was observed in almost all BICR31 cells. (scirp.org)
  • Most DOK cells showed no localization of plectin-1, but strong reactions were detected in the cytoplasm of BICR31 cells. (scirp.org)
  • Stationary phase cells were either exposed to 100-600 Gy of 60 Co gamma radiation or treated with 0.15-0.3 μM 4-NQO (30°C, 1 hour), after which they were plated on synthetic complete or minimal media with or without caffeine. (begellhouse.com)
  • In contrast, caffeine was found to increase the induced gene conversion frequency when cells treated with 0.15, 0.225, and 0.3 μM 4-NQO were plated on media containing caffeine. (begellhouse.com)
  • Both amines are available in the diet and putrescine can also be generated from higher polyamines via the SSAT (spermidine/spermine N 1 -acetyltransferase)/AcPAO (acetylpolyamine oxidase) system, excreted from donor cells and then taken back up by other cells. (portlandpress.com)
  • and (I) individual cells invading the interface (score 3) (1). (spandidos-publications.com)
  • Aqueous root extract (ARE) of Inula racemosa (100, 200 and 400 mg/kg bw) with and without 4-NQO along with vehicle control (H 2 O) were administered orally for five consecutive days. (hindawi.com)
  • The results indicate that the posttreatment repair processes following gamma irradiation or 4-NQO treatment are modified via different pathways. (begellhouse.com)
  • 1 Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY 10016, USA. (sciencemag.org)
  • Several mechanisms for reaction of 4-NQO with intracellular NPSH were indicated. (aacrjournals.org)
  • Among the inositol phosphates, inositol 1,4,5-trisphosphate is best known for its release of intracellular calcium ( 1 ). (pnas.org)