An enzyme that catalyzes the hydrolysis of nitrophenyl phosphates to nitrophenols. At acid pH it is probably ACID PHOSPHATASE (EC 3.1.3.2); at alkaline pH it is probably ALKALINE PHOSPHATASE (EC 3.1.3.1). EC 3.1.3.41.

Hyperaldosteronemia in rabbits inhibits the cardiac sarcolemmal Na(+)-K(+) pump. (1/86)

Aldosterone upregulates the Na(+)-K(+) pump in kidney and colon, classical target organs for the hormone. An effect on pump function in the heart is not firmly established. Because the myocardium contains mineralocorticoid receptors, we examined whether aldosterone has an effect on Na(+)-K(+) pump function in cardiac myocytes. Myocytes were isolated from rabbits given aldosterone via osmotic minipumps and from controls. Electrogenic Na(+)-K(+) pump current, arising from the 3:2 Na(+):K(+) exchange ratio, was measured in single myocytes using the whole-cell patch clamp technique. Treatment with aldosterone induced a decrease in pump current measured when myocytes were dialyzed with patch pipette solution containing Na(+) in a concentration of 10 mmol/L, whereas there was no effect measured when the solution contained 80 mmol/L Na(+). Aldosterone had no effect on myocardial Na(+)-K(+) pump concentration evaluated by vanadate-facilitated [(3)H]ouabain binding or by K(+)-dependent paranitrophenylphosphatase activity in crude homogenates. Aldosterone induced an increase in intracellular Na(+) activity. The aldosterone-induced decrease in pump current and increased intracellular Na(+) were prevented by cotreatment with the mineralocorticoid receptor antagonist spironolactone. Our results indicate that hyperaldosteronemia decreases the apparent Na(+) affinity of the Na(+)-K(+) pump, whereas it has no effect on maximal pump capacity.  (+info)

Alphabeta protomers of Na+,K+-ATPase from microsomes of duck salt gland are mostly monomeric: formation of higher oligomers does not modify molecular activity. (2/86)

The distance that separates alphabeta protomers of the Na(+), K(+)-ATPase in microsomes and in purified membranes prepared from duck nasal salt glands was estimated by measuring fluorescence resonance energy transfer between anthroylouabain bound to a population of alphabeta protomers and either N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl]-6-aminohexyl ouabain or 5-(and-6)-carboxyfluorescein-6-aminohexyl ouabain bound to the rest. Energy transfer between probes bound in the microsomal preparation was less than in the purified membranes. The efficiency of energy transfer between anthroylouabain and N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl]-6-aminohexyl ouabain was 29.2% in the microsomes compared with 62.6% in the purified preparation. Similar results were obtained with 5-(and-6)-carboxyfluorescein-6-aminohexyl ouabain as acceptor. We calculate that either the protomer bound probes were on the average 13 A farther apart in the microsomes than in the purified membranes, or that 53% of the protomers are monomeric in the microsome preparation. Microsomes prepared in the presence of phalloidin (a toxin that binds to F actin and stabilizes the actin-based cytoskeleton) showed less quench than those prepared in its absence. The data support the hypothesis that protomers are kept apart by their association with the cytoskeleton. The turnover rate while hydrolyzing ATP is the same in the microsomal and purified preparations; higher oligomer formation has no significant effect on the enzyme reaction mechanism.  (+info)

HMG CoA reductase inhibition reduces sarcolemmal Na(+)-K(+) pump density. (3/86)

OBJECTIVES: HMG CoA reductase inhibitors reduce cellular availability of mevalonate, a precursor in cholesterol synthesis. Since the cholesterol content of cell membranes is an important determinant of Na(+)-K(+) pump function we speculated that treatment with HMG CoA reductase inhibitors affects Na(+)-K(+) pump activity. METHODS: We treated rabbits and rats for 2 weeks with the HMG CoA reductase inhibitor lovastatin and measured Na(+)-K(+) pump current (I(p)) in isolated rabbit cardiac myocytes using the whole cell patch-clamp technique, K-dependent p-nitrophenyl phosphatase (p-NPPase) activity in crude myocardial and skeletal muscle homogenates, and vanadate-facilitated 3H-ouabain binding in intact skeletal muscle samples from rats. RESULTS: Treatment with lovastatin caused statistically significant reductions in I(p), myocardial and skeletal muscle K-dependent p-NPPase activity and 3H-ouabain binding in the myocardium and skeletal muscle. The lovastatin-induced decrease in I(p) was eliminated by parenteral co-administration of mevalonate. However, this was not related to cardiac cholesterol content. CONCLUSIONS: Treatment with lovastatin reduces Na(+)-K(+) pump activity and abundance in rabbit and rat sarcolemma.  (+info)

Structural and immunological similarities between high molecular weight zinc ion-dependent p-nitrophenylphosphatase and fructose-1,6-bisphosphate aldolase from bovine liver. (4/86)

High molecular weight zinc ion-dependent acid p-nitrophenylphosphatase (HMW-ZnAPase) was purified from bovine liver to homogeneity as judged by native and sodium dodecyl sulfate polyacrylamide gel electrophoresis. The partial sequence of the purified enzyme electroblotted on PVDF membrane reveals a 95% sequence homology with human and bovine liver fructose-1,6-bisphosphate aldolase isozyme B (FALD B). FALD B was isolated from bovine liver using an affinity elution from phosphocellulose column. FALD B from bovine liver shows a native and subunit molecular weight that is indistinguishable from that of HMW-ZnAPase. In addition, an affinity purified antiserum raised in rabbits against purified HMW-ZnAPase cross-reacts with bovine liver FALD B and rabbit muscle isozymes. Despite these similarities, HMW-ZnAPase does not show FALD activity and bovine liver FALD does not display any zinc ion-p-nitrophenylphosphatase activity. These results suggested the existence of structural and immunological similarities between bovine liver HMW-ZnAPase and FALD B. Differences in some amino acid residues in enzyme activity indicate that they may be involved in different biochemical functions.  (+info)

p-Nitrophenylphosphatase activity catalyzed by plasma membrane (Ca(2+) + Mg(2+)ATPase: correlation with structural changes modulated by glycerol and Ca(2+). (5/86)

The plasma membrane (Ca(2+) + Mg(2+))ATPase hydrolyzes pseudo-substrates such as p-nitrophenylphosphate. Except when calmodulin is present, Ca(2+) ions inhibit the p-nitrophenylphosphatase activity. In this report it is shown that, in the presence of glycerol, Ca(2+) strongly stimulates phosphatase activity in a dose-dependent manner. The glycerol- and Ca(2+)-induced increase in activity is correlated with modifications in the spectral center of mass (average emission wavenumber) of the intrinsic fluorescence of the enzyme. It is concluded that the synergistic effect of glycerol and Ca(2+) is related to opposite long-term hydration effects on the substrate binding domain and the Ca(2+) binding domain.  (+info)

Characterization of a novel mammalian phosphatase having sequence similarity to Schizosaccharomyces pombe PHO2 and Saccharomyces cerevisiae PHO13. (6/86)

p34, a specific p-nitrophenyl phosphatase (pNPPase) was identified and purified from the murine cell line EL4 in a screen for the intracellular molecular targets of the antiinflammatory natural product parthenolide. A BLAST search analysis revealed that it has a high degree of sequence similarity to two yeast alkaline phosphatases. We have cloned, sequenced, and expressed p34 as a GST-tagged fusion protein in Escherichia coli and an EE-epitope-tagged fusion protein in mammalian cells. Using p-nitrophenyl phosphate (pNPP) as a substrate, p34 is optimally active at pH 7.6 with a K(m) of 1.36 mM and K(cat) of 0.052 min(-1). Addition of 1 mM Mg(2+) to the reaction mixture increases its activity by 14-fold. Other divalent metal ions such as Co(2+) and Mn(2+) also stimulated the activity of the enzyme, while Zn(2+), Fe(2+), and Cu(2+) had no effect. Furthermore, both NaCl and KCl enhanced the activity of the enzyme, having maximal effect at 50 and 75 mM, respectively. The enzyme is inhibited by sodium orthovanadate but not by sodium fluoride or okadaic acid. Mutational analysis data suggest that p34 belongs to the group of phosphatases characterized by the sequence motif DXDX(T/V).  (+info)

Transepithelial potential in mesonephric nephrons of 7-day-old chick embryos in relation to the histochemically detected sodium pump. (7/86)

In order to obtain basic information on the transport properties of differentiating embryonic nephrons, we examined the 7-day-old chick mesonephros by measuring the transtubular epithelial potential difference (TPD) and by histochemical detection of Na,K-ATPase activity. TPD as an indicator of the electrogenic transport was measured in individual segments of superficial nephrons in vivo. Their electric polarity was always lumen-negative. TPD was reduced by addition of 10 mM KCN applied to the mesonephric nephrons from the outside. In the proximal tubules, TPD was significantly lower (mean+/-SD: -1.0+/-0.5 mV) than in the distal and collecting tubules (-2.2+/-1.0 mV, p< or =0.05). Activity of the sodium pump was evaluated histochemically by detection of ouabain-sensitive potassium-dependent p-nitrophenyl phosphatase in cryostat sections of the mesonephros. The enzyme activity was demonstrated only in distal tubules and in the collecting ducts, but not in the proximal tubules. These findings have revealed significant differences between embryonic nephron segments: the distal tubule, in contrast to the proximal one, is supplied by the sodium pump and is able to generate higher TPD. Therefore, we consider that it is only the distal nephron, which possesses the ability of active transport.  (+info)

Cytochemistry of protein kinase C and Na-K-ATPase in rabbit ciliary processes treated with phorbol ester. (8/86)

Immunocytochemical localization of protein kinase C (PKC) in rabbit ciliary processes was investigated using anti-PKC monoclonal antibodies (MAbs) against rabbit Types 1, 2, and 3 PKC. Specific immunolabeling was observed in nonpigmented epithelial (NPE) cells and in the capillaries of the ciliary processes with anti-Types 2 and 3 MAbs. No apparent staining was seen with anti-Type 1 MAbs. Immunoelectron microscopy of Types 2 and 3 MAbs revealed a diffuse distribution of immunoreactive PKC in the cytoplasm, in the nucleus, and on the plasma membrane in the NPE cells. When incubated with phorbol 12-myristate 13-acetate (PMA), the distribution of PKC was basically similar to that of the untreated group. However, the labelling density on the plasma membrane at basolateral interdigitation increased considerably for anti-Types 2 and 3 PKC MAbs. In addition, the enzyme cytochemical activity of Na-K-ATPase (ouabain-sensitive K-NPPase) and its change after PMA administration in the ciliary processes were observed. An intense reaction was seen on the basolateral plasma membrane of the NPE cells. In the PMA-treated group, the enzyme activity of Na-K-ATPase apparently was decreased. These findings provide evidence that PKC plays a crucial role in the function of the NPE cells of the ciliary processes, possibly in aqueous humor production.  (+info)

4-Nitrophenylphosphatase is an enzyme that catalyzes the hydrolysis of 4-nitrophenyl phosphate, producing 4-nitrophenol and phosphate. This enzyme is commonly used in laboratory assays to measure enzyme activity or to determine the presence of certain metals, such as aluminum and lead, which can inhibit its activity. The hydrolysis reaction results in the formation of yellow 4-nitrophenol, which can be easily measured spectrophotometrically at a wavelength of 405 nm. The activity of 4-nitrophenylphosphatase is often used as an indicator of the functional status of certain organelles, such as lysosomes, in biological systems.

Other names in common use include nitrophenyl phosphatase, p-nitrophenylphosphatase, para-nitrophenyl phosphatase, K-pNPPase, ... Attias J, Bonnet JL (1972). "A specific alkaline p-nitrophenylphosphatase activity from baker's yeast". Biochim. Biophys. Acta ... Attias J, Durand H (1973). "Further characterization of a specific p-nitrophenylphosphatase from baker's yeast". Biochim. ... NPPase, PNPPase, Ecto-p-nitrophenyl phosphatase, and p-nitrophenylphosphate phosphohydrolase. This enzyme participates in γ- ...
4-beta-glucosidase MeSH D08.811.277.450.420.200.600 - glucan endo-1,3-beta-d-glucosidase MeSH D08.811.277.450.420.375 - glucan ... map kinase kinase 4 MeSH D08.811.913.696.620.682.700.565.500 - map kinase kinase 5 MeSH D08.811.913.696.620.682.700.565.600 - ... map kinase kinase 4 MeSH D08.811.913.696.620.682.725.200.500 - map kinase kinase 5 MeSH D08.811.913.696.620.682.725.200.600 - ... type 4 MeSH D08.811.913.696.620.682.725.400.050 - proto-oncogene proteins c-kit MeSH D08.811.913.696.620.682.725.400.075 - ...
EC 3.2.1.91: cellulose 1,4-β-cellobiosidase (non-reducing end) EC 3.2.1.92: peptidoglycan β-N-acetylmuramidase EC 3.2.1.93: α,α ... 4-β-xylosidase EC 3.2.1.38: β-D-fucosidase EC 3.2.1.39: glucan endo-1,3-β-D-glucosidase EC 3.2.1.40: α-L-rhamnosidase EC 3.2. ... 4-α-glucosidase EC 3.2.1.4: cellulase EC 3.2.1.5: deleted EC 3.2.1.6: endo-1,3(4)-β-glucanase EC 3.2.1.7: inulinase EC 3.2.1.8 ... 4-β-xylanase EC 3.2.1.137: mannan exo-1,2-1,6-α-mannosidase EC 3.2.1.138: Now EC 4.2.2.15, anhydrosialidase EC 3.2.1.139: α- ...
NIPSNAP14-Nitrophenylphosphatase Domain and Non-Neuronal SNAP25-Like Protein Homolog 1; Numb: Drosophila mutation that removes ... 1773 (4): 565-76. doi:10.1016/j.bbamcr.2007.01.001. PMID 17292493. Zhang SS, Xie X, Wen J, Luo KJ, Liu QW, Yang H, et al. ( ... 78 (4): 274-82. doi:10.1002/mrd.21303. PMID 21400627. S2CID 21140465. De Clercq K, Van den Eynde C, Hennes A, Van Bree R, Voets ... 57 (4): 427-50. doi:10.1124/pr.57.4.6. PMID 16382100. S2CID 17936350. Müller D, Hoenderop JG, Merkx GF, van Os CH, Bindels RJ ( ...
Other names in common use include nitrophenyl phosphatase, p-nitrophenylphosphatase, para-nitrophenyl phosphatase, K-pNPPase, ... Attias J, Bonnet JL (1972). "A specific alkaline p-nitrophenylphosphatase activity from bakers yeast". Biochim. Biophys. Acta ... Attias J, Durand H (1973). "Further characterization of a specific p-nitrophenylphosphatase from bakers yeast". Biochim. ... NPPase, PNPPase, Ecto-p-nitrophenyl phosphatase, and p-nitrophenylphosphate phosphohydrolase. This enzyme participates in γ- ...
Properties of potassium activatedp-nitrophenyl phosphatase of plasma membranes isolated from rat stomach muscle Academic ... smooth muscle of rat gastric fundus was found to contain a substantial level of potassium-stimulated p-nitrophenylphosphatase ...
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. ... ATP and 4-nitrophenyl phosphatase. It is detected in human alveolar macrophages, osteoclasts, spleen and liver. Its expression ...
para Nitrophenyl Phosphatase para Nitrophenylphosphatase para-Nitrophenyl Phosphatase para-Nitrophenylphosphatase K-p NPPase - ... para Nitrophenyl Phosphatase. para Nitrophenylphosphatase. para-Nitrophenyl Phosphatase. para-Nitrophenylphosphatase. Tree ... Nitrophenyl Phosphatase. p NPPase. p Nitrophenylphosphatase. p-NPPase. p-Nitrophenylphosphatase. p-Nitrophenylphosphatase, K- ... K-Dependent p-Nitrophenylphosphatase p-Nitrophenylphosphatase, K-Dependent Nitrophenyl Phosphatase - Broader Concept UI. ...
Ensembl Metazoa is a genome-centric portal for metazoan species of scientific interest
This graph shows the total number of publications written about "Phosphatidate Phosphatase" by people in Harvard Catalyst Profiles by year, and whether "Phosphatidate Phosphatase" was a major or minor topic of these publication ...
4-nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1 (C. elegans). ...
4-nitrophenylphosphatase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PHO13 PE=1 SV=2. 11. 302. 4.0E-69. ... 4-nitrophenylphosphatase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PHO13 PE=1 SV=2. 11. 302. 4.0E-69. ... 4-nitrophenylphosphatase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=pho2 PE=4 SV=2. 18. 306. 8.0E-94. ... 4-nitrophenylphosphatase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=pho2 PE=4 SV=2. 18. 306. 8.0E-94. ...
Descritores em Ciências da Saúde
Nesterovitch AB, Gyorfy Z, Hoffman MD, Moore EC, Elbuluk N, Tryniszewska B, Rauch TA, Simon M, Kang S, Fisher GJ, Mikecz K, Tharp MD, Glant TT. Alteration in the gene encoding protein tyrosine phosphatase nonreceptor type 6 (PTPN6/SHP1) may contribute to neutrophilic dermatoses. Am J Pathol. 2011 Apr; 178(4):1434-41 ...
K Dependent p Nitrophenylphosphatase use 4-Nitrophenylphosphatase K Epoxidase, Vitamin use Vitamin K Epoxide Reductases ... Kaposis Sarcoma Fibroblast Growth Factor use Fibroblast Growth Factor 4 Kaposis Sarcoma-Associated Herpesvirus use ... Kinase, Phosphatidylinositiol use 1-Phosphatidylinositol 4-Kinase Kinase, Phosphatidylinositol-3-OH use Phosphatidylinositol 3- ... Killer Cell Lectin Like Receptor Subfamily C, Member 4 use NK Cell Lectin-Like Receptor Subfamily C ...
K Dependent p Nitrophenylphosphatase use 4-Nitrophenylphosphatase K Epoxidase, Vitamin use Vitamin K Epoxide Reductases ... Kaposis Sarcoma Fibroblast Growth Factor use Fibroblast Growth Factor 4 Kaposis Sarcoma-Associated Herpesvirus use ... Kinase, Phosphatidylinositiol use 1-Phosphatidylinositol 4-Kinase Kinase, Phosphatidylinositol-3-OH use Phosphatidylinositol 3- ... Killer Cell Lectin Like Receptor Subfamily C, Member 4 use NK Cell Lectin-Like Receptor Subfamily C ...
K Dependent p Nitrophenylphosphatase use 4-Nitrophenylphosphatase K Epoxidase, Vitamin use Vitamin K Epoxide Reductases ... Kaposis Sarcoma Fibroblast Growth Factor use Fibroblast Growth Factor 4 Kaposis Sarcoma-Associated Herpesvirus use ... Killer Cell Lectin Like Receptor Subfamily C, Member 4 use NK Cell Lectin-Like Receptor Subfamily C ... Killer Cell Lectin-Like Receptor Subfamily C, Member 4 use NK Cell Lectin-Like Receptor Subfamily C ...
p Nitrophenylphosphatase use 4-Nitrophenylphosphatase p NPPase use 4-Nitrophenylphosphatase P Oxides, Cyclic use Cyclic P- ... p48 Subunit, Chromatin Assembly Factor 1 use Retinoblastoma-Binding Protein 4 p48 Subunit, Chromatin Assembly Factor-1 use ... p48-Binding Protein, Retinoblastoma use Retinoblastoma-Binding Protein 4 p49 3F12 Kinase use Mitogen-Activated Protein Kinase ... P Hydroxyphenylpyruvate Oxidase use 4-Hydroxyphenylpyruvate Dioxygenase p Methoxy N methylphenethylamine use p-Methoxy-N- ...
Van Vleet JH, Jeffries TW, Olsson L. Deleting the para-nitrophenyl phosphatase (pNPPase), PHO13, in recombinant Saccharomyces ... 4. Aerobic growth in 96 wells micro-titer plates of IMX730-△H (A) and IMX730-pGAL::XKS1 (B) in mineral medium containing 0.5% d ... 4A). In contrast, the IMX730-pGAL::XKS1 strain not only showed a reduced lag phase as compared to the IMX730 strain but was ... ZnSO4·7H2O, 4.5 mg/L; CoCl2·6H2O, 0.3 mg/L; MnCl2·2H2O, 0.84 mg/L; CuSO4·5H2O, 0.3 mg/L; CaCl2·2H2O, 4.5 mg/L; FeSO4·7H2O, 3.0 ...
... potassium-dependent p-nitrophenylphosphatase activity. Histochemistry 67(2): 125-138. https://doi.org/10.1007/BF00493231 ... Figure 4. Ultrastructure of the cuticle in the anterior chamber and the papillate region. A The cuticle in the anterior chamber ... 4. ). The basal labyrinth is mainly between 1 and 4 µm deep in the anterior chamber and between 3 and 10 µm deep in the ... Supplementary material 4 SI Figure 4. Boxplots depicting individual measurements of apical membrane labyrinth depth ...
4 Hydroxyphenylpyruvate Dioxygenase Deficiency Disease use Tyrosinemias 4-Nitrophenol-2-Hydroxylase use Cytochrome P-450 CYP2E1 ... 3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer ... 2H-Benzo(a)quinolizin-2-ol, 2-Ethyl-1,3,4,6,7,11b-hexahydro-3-isobutyl-9,10-dimethoxy- ... 3-Keto-5-alpha-Steroid delta-4-Dehydrogenase use Testosterone 5-alpha-Reductase ...
3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer ... 2H-Benzo(a)quinolizin-2-ol, 2-Ethyl-1,3,4,6,7,11b-hexahydro-3-isobutyl-9,10-dimethoxy- ... 3-Keto-5-alpha-Steroid delta-4-Dehydrogenase use Testosterone 5-alpha-Reductase ... 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester ...
4 Hydroxyphenylpyruvate Dioxygenase Deficiency Disease use Tyrosinemias 4-Nitrophenol-2-Hydroxylase use Cytochrome P-450 CYP2E1 ... 3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer ... 2H-Benzo(a)quinolizin-2-ol, 2-Ethyl-1,3,4,6,7,11b-hexahydro-3-isobutyl-9,10-dimethoxy- ... 3-Keto-5-alpha-Steroid delta-4-Dehydrogenase use Testosterone 5-alpha-Reductase ...
3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer ... 2H-Benzo(a)quinolizin-2-ol, 2-Ethyl-1,3,4,6,7,11b-hexahydro-3-isobutyl-9,10-dimethoxy- ... 3-Keto-5-alpha-Steroid delta-4-Dehydrogenase use Testosterone 5-alpha-Reductase ... 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester ...
3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer ... 2H-Benzo(a)quinolizin-2-ol, 2-Ethyl-1,3,4,6,7,11b-hexahydro-3-isobutyl-9,10-dimethoxy- ... 3-Keto-5-alpha-Steroid delta-4-Dehydrogenase use 3-Oxo-5-alpha-Steroid 4-Dehydrogenase ... 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester ...
3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer ... 2H-Benzo(a)quinolizin-2-ol, 2-Ethyl-1,3,4,6,7,11b-hexahydro-3-isobutyl-9,10-dimethoxy- ... 3-Keto-5-alpha-Steroid delta-4-Dehydrogenase use 3-Oxo-5-alpha-Steroid 4-Dehydrogenase ... 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester ...
3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer ... 2H-Benzo(a)quinolizin-2-ol, 2-Ethyl-1,3,4,6,7,11b-hexahydro-3-isobutyl-9,10-dimethoxy- ... 3-Keto-5-alpha-Steroid delta-4-Dehydrogenase use Testosterone 5-alpha-Reductase ... 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester ...
3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer ... 2H-Benzo(a)quinolizin-2-ol, 2-Ethyl-1,3,4,6,7,11b-hexahydro-3-isobutyl-9,10-dimethoxy- ... 3-Keto-5-alpha-Steroid delta-4-Dehydrogenase use Testosterone 5-alpha-Reductase ... 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester ...
ATP and cyclic AMP were competitive inhibitors of bis-(p-nitrophenyl) phosphatase activity of the enzyme and Ki values (25 mM ... While orthovanadate abolished p-nitrophenylphosphatase activity, ouabain inhibition reached 80% (KI=304.9+/-18.3 micromol L-1 ... 4. Regulation by the exogenous polyamine spermidine of Na,K-ATPase activity from the gills of the euryhaline swimming crab ... 4. A model for the action of zinc and magnesium ions on the modulation of the enzyme activity is proposed. ...
Berglund O, Sehlin J & T ljedal I-B: 86Rb+ fluxes and K+-stimulated nitrophenyl phosphatase activity in the pancreatic islets ... 4. R kstopp och lokal s rv rd - v rdstrategi som f rb ttrat behandlingsresultaten hos diabetiker: forskning och utbildning i ... 4. T ljedal I-B & Hellman B: Morphological characteristics of the epididymal adipose tissue in different types of hereditary ... Filosofisk tidskrift 2002(4):11-17.. 8. Strong holism, weak holism, and health. Medicine, Health Care and Philosophy 7:143-148 ...
  • Tartrate-resistant acid phosphatase (TRAP) is a basic iron-binding protein with high activity towards phosphoproteins, ATP and 4-nitrophenyl phosphatase. (thermofisher.com)
  • The systematic name of this enzyme class is 4-nitrophenylphosphate phosphohydrolase. (wikipedia.org)
  • The enzyme 4-nitrophenylphosphatase (EC 3.1.3.41) catalyzes the reaction 4-nitrophenyl phosphate + H2O ⇌ {\displaystyle \rightleftharpoons } 4-nitrophenol + phosphate This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. (wikipedia.org)
  • The plasma membrane-enriched fraction isolated from smooth muscle of rat gastric fundus was found to contain a substantial level of potassium-stimulated p-nitrophenylphosphatase activity (K-pNPPase), and its subcellular distribution closely resembled that of other plasma membrane enzyme markers. (mcmaster.ca)
  • However, we observed an 89% suppression of the activity of K+ -stimulated ouabain-sensitive p-nitrophenylphosphatase (pNPPase), an enzyme marker for the Na+ -K+ pump, in plasma membranes from infected compared with control rats. (mcmaster.ca)