An enzyme that catalyzes the hydrolysis of nitrophenyl phosphates to nitrophenols. At acid pH it is probably ACID PHOSPHATASE (EC 3.1.3.2); at alkaline pH it is probably ALKALINE PHOSPHATASE (EC 3.1.3.1). EC 3.1.3.41.

Hyperaldosteronemia in rabbits inhibits the cardiac sarcolemmal Na(+)-K(+) pump. (1/86)

Aldosterone upregulates the Na(+)-K(+) pump in kidney and colon, classical target organs for the hormone. An effect on pump function in the heart is not firmly established. Because the myocardium contains mineralocorticoid receptors, we examined whether aldosterone has an effect on Na(+)-K(+) pump function in cardiac myocytes. Myocytes were isolated from rabbits given aldosterone via osmotic minipumps and from controls. Electrogenic Na(+)-K(+) pump current, arising from the 3:2 Na(+):K(+) exchange ratio, was measured in single myocytes using the whole-cell patch clamp technique. Treatment with aldosterone induced a decrease in pump current measured when myocytes were dialyzed with patch pipette solution containing Na(+) in a concentration of 10 mmol/L, whereas there was no effect measured when the solution contained 80 mmol/L Na(+). Aldosterone had no effect on myocardial Na(+)-K(+) pump concentration evaluated by vanadate-facilitated [(3)H]ouabain binding or by K(+)-dependent paranitrophenylphosphatase activity in crude homogenates. Aldosterone induced an increase in intracellular Na(+) activity. The aldosterone-induced decrease in pump current and increased intracellular Na(+) were prevented by cotreatment with the mineralocorticoid receptor antagonist spironolactone. Our results indicate that hyperaldosteronemia decreases the apparent Na(+) affinity of the Na(+)-K(+) pump, whereas it has no effect on maximal pump capacity.  (+info)

Alphabeta protomers of Na+,K+-ATPase from microsomes of duck salt gland are mostly monomeric: formation of higher oligomers does not modify molecular activity. (2/86)

The distance that separates alphabeta protomers of the Na(+), K(+)-ATPase in microsomes and in purified membranes prepared from duck nasal salt glands was estimated by measuring fluorescence resonance energy transfer between anthroylouabain bound to a population of alphabeta protomers and either N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl]-6-aminohexyl ouabain or 5-(and-6)-carboxyfluorescein-6-aminohexyl ouabain bound to the rest. Energy transfer between probes bound in the microsomal preparation was less than in the purified membranes. The efficiency of energy transfer between anthroylouabain and N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl]-6-aminohexyl ouabain was 29.2% in the microsomes compared with 62.6% in the purified preparation. Similar results were obtained with 5-(and-6)-carboxyfluorescein-6-aminohexyl ouabain as acceptor. We calculate that either the protomer bound probes were on the average 13 A farther apart in the microsomes than in the purified membranes, or that 53% of the protomers are monomeric in the microsome preparation. Microsomes prepared in the presence of phalloidin (a toxin that binds to F actin and stabilizes the actin-based cytoskeleton) showed less quench than those prepared in its absence. The data support the hypothesis that protomers are kept apart by their association with the cytoskeleton. The turnover rate while hydrolyzing ATP is the same in the microsomal and purified preparations; higher oligomer formation has no significant effect on the enzyme reaction mechanism.  (+info)

HMG CoA reductase inhibition reduces sarcolemmal Na(+)-K(+) pump density. (3/86)

OBJECTIVES: HMG CoA reductase inhibitors reduce cellular availability of mevalonate, a precursor in cholesterol synthesis. Since the cholesterol content of cell membranes is an important determinant of Na(+)-K(+) pump function we speculated that treatment with HMG CoA reductase inhibitors affects Na(+)-K(+) pump activity. METHODS: We treated rabbits and rats for 2 weeks with the HMG CoA reductase inhibitor lovastatin and measured Na(+)-K(+) pump current (I(p)) in isolated rabbit cardiac myocytes using the whole cell patch-clamp technique, K-dependent p-nitrophenyl phosphatase (p-NPPase) activity in crude myocardial and skeletal muscle homogenates, and vanadate-facilitated 3H-ouabain binding in intact skeletal muscle samples from rats. RESULTS: Treatment with lovastatin caused statistically significant reductions in I(p), myocardial and skeletal muscle K-dependent p-NPPase activity and 3H-ouabain binding in the myocardium and skeletal muscle. The lovastatin-induced decrease in I(p) was eliminated by parenteral co-administration of mevalonate. However, this was not related to cardiac cholesterol content. CONCLUSIONS: Treatment with lovastatin reduces Na(+)-K(+) pump activity and abundance in rabbit and rat sarcolemma.  (+info)

Structural and immunological similarities between high molecular weight zinc ion-dependent p-nitrophenylphosphatase and fructose-1,6-bisphosphate aldolase from bovine liver. (4/86)

High molecular weight zinc ion-dependent acid p-nitrophenylphosphatase (HMW-ZnAPase) was purified from bovine liver to homogeneity as judged by native and sodium dodecyl sulfate polyacrylamide gel electrophoresis. The partial sequence of the purified enzyme electroblotted on PVDF membrane reveals a 95% sequence homology with human and bovine liver fructose-1,6-bisphosphate aldolase isozyme B (FALD B). FALD B was isolated from bovine liver using an affinity elution from phosphocellulose column. FALD B from bovine liver shows a native and subunit molecular weight that is indistinguishable from that of HMW-ZnAPase. In addition, an affinity purified antiserum raised in rabbits against purified HMW-ZnAPase cross-reacts with bovine liver FALD B and rabbit muscle isozymes. Despite these similarities, HMW-ZnAPase does not show FALD activity and bovine liver FALD does not display any zinc ion-p-nitrophenylphosphatase activity. These results suggested the existence of structural and immunological similarities between bovine liver HMW-ZnAPase and FALD B. Differences in some amino acid residues in enzyme activity indicate that they may be involved in different biochemical functions.  (+info)

p-Nitrophenylphosphatase activity catalyzed by plasma membrane (Ca(2+) + Mg(2+)ATPase: correlation with structural changes modulated by glycerol and Ca(2+). (5/86)

The plasma membrane (Ca(2+) + Mg(2+))ATPase hydrolyzes pseudo-substrates such as p-nitrophenylphosphate. Except when calmodulin is present, Ca(2+) ions inhibit the p-nitrophenylphosphatase activity. In this report it is shown that, in the presence of glycerol, Ca(2+) strongly stimulates phosphatase activity in a dose-dependent manner. The glycerol- and Ca(2+)-induced increase in activity is correlated with modifications in the spectral center of mass (average emission wavenumber) of the intrinsic fluorescence of the enzyme. It is concluded that the synergistic effect of glycerol and Ca(2+) is related to opposite long-term hydration effects on the substrate binding domain and the Ca(2+) binding domain.  (+info)

Characterization of a novel mammalian phosphatase having sequence similarity to Schizosaccharomyces pombe PHO2 and Saccharomyces cerevisiae PHO13. (6/86)

p34, a specific p-nitrophenyl phosphatase (pNPPase) was identified and purified from the murine cell line EL4 in a screen for the intracellular molecular targets of the antiinflammatory natural product parthenolide. A BLAST search analysis revealed that it has a high degree of sequence similarity to two yeast alkaline phosphatases. We have cloned, sequenced, and expressed p34 as a GST-tagged fusion protein in Escherichia coli and an EE-epitope-tagged fusion protein in mammalian cells. Using p-nitrophenyl phosphate (pNPP) as a substrate, p34 is optimally active at pH 7.6 with a K(m) of 1.36 mM and K(cat) of 0.052 min(-1). Addition of 1 mM Mg(2+) to the reaction mixture increases its activity by 14-fold. Other divalent metal ions such as Co(2+) and Mn(2+) also stimulated the activity of the enzyme, while Zn(2+), Fe(2+), and Cu(2+) had no effect. Furthermore, both NaCl and KCl enhanced the activity of the enzyme, having maximal effect at 50 and 75 mM, respectively. The enzyme is inhibited by sodium orthovanadate but not by sodium fluoride or okadaic acid. Mutational analysis data suggest that p34 belongs to the group of phosphatases characterized by the sequence motif DXDX(T/V).  (+info)

Transepithelial potential in mesonephric nephrons of 7-day-old chick embryos in relation to the histochemically detected sodium pump. (7/86)

In order to obtain basic information on the transport properties of differentiating embryonic nephrons, we examined the 7-day-old chick mesonephros by measuring the transtubular epithelial potential difference (TPD) and by histochemical detection of Na,K-ATPase activity. TPD as an indicator of the electrogenic transport was measured in individual segments of superficial nephrons in vivo. Their electric polarity was always lumen-negative. TPD was reduced by addition of 10 mM KCN applied to the mesonephric nephrons from the outside. In the proximal tubules, TPD was significantly lower (mean+/-SD: -1.0+/-0.5 mV) than in the distal and collecting tubules (-2.2+/-1.0 mV, p< or =0.05). Activity of the sodium pump was evaluated histochemically by detection of ouabain-sensitive potassium-dependent p-nitrophenyl phosphatase in cryostat sections of the mesonephros. The enzyme activity was demonstrated only in distal tubules and in the collecting ducts, but not in the proximal tubules. These findings have revealed significant differences between embryonic nephron segments: the distal tubule, in contrast to the proximal one, is supplied by the sodium pump and is able to generate higher TPD. Therefore, we consider that it is only the distal nephron, which possesses the ability of active transport.  (+info)

Cytochemistry of protein kinase C and Na-K-ATPase in rabbit ciliary processes treated with phorbol ester. (8/86)

Immunocytochemical localization of protein kinase C (PKC) in rabbit ciliary processes was investigated using anti-PKC monoclonal antibodies (MAbs) against rabbit Types 1, 2, and 3 PKC. Specific immunolabeling was observed in nonpigmented epithelial (NPE) cells and in the capillaries of the ciliary processes with anti-Types 2 and 3 MAbs. No apparent staining was seen with anti-Type 1 MAbs. Immunoelectron microscopy of Types 2 and 3 MAbs revealed a diffuse distribution of immunoreactive PKC in the cytoplasm, in the nucleus, and on the plasma membrane in the NPE cells. When incubated with phorbol 12-myristate 13-acetate (PMA), the distribution of PKC was basically similar to that of the untreated group. However, the labelling density on the plasma membrane at basolateral interdigitation increased considerably for anti-Types 2 and 3 PKC MAbs. In addition, the enzyme cytochemical activity of Na-K-ATPase (ouabain-sensitive K-NPPase) and its change after PMA administration in the ciliary processes were observed. An intense reaction was seen on the basolateral plasma membrane of the NPE cells. In the PMA-treated group, the enzyme activity of Na-K-ATPase apparently was decreased. These findings provide evidence that PKC plays a crucial role in the function of the NPE cells of the ciliary processes, possibly in aqueous humor production.  (+info)

1. These experiments examined the effects of a high NaCl diet on (Na+, K+)-ATPase in kidney, heart and cerebral cortex, on the level of circulating inhibitor of (Na+, K+)-ATPase in plasma, and on stimulation of (Na+, K+)-ATPase by treatment with dextro (d)-amphetamine.. 2. High salt diet increased indices of (Na+, K+)-ATPase activity (K+-activated p-nitrophenylphosphatase activity and ouabain binding) in kidney medulla, prevented stimulation by amphetamine in cerebral cortex and reduced amphetamine stimulation in heart.. 3. High NaCl feeding increased the plasma level of circulating inhibitor of (Na+, K+)-ATPase.. 4. Amphetamine alone had no effect on inhibitor level but amphetamine administration reduced the increase in inhibitor with high NaCl feeding.. ...
The NIPSNAP proteins comprise a family of evolutionarily well-conserved proteins with strong sequence similarity to the central portion of a protein encoded by C. elegans chromosome III between a 4-nitrophenylphosphatase (NIP) domain and non-neuronal SNAP25-like protein. NIPSNAP2, a novel gene encoding a protein with tyrosine phosphorylation sites and a transmembrane domain, is co-amplified with EGFR in approximately 40% of glioblastomas, the most common and malignant form of central nervous system tumors. While NIPSNAP3B is highly expressed skeletal muscle, NIPSNAP3A mRNA levels are low. NIPSNAP3A protein is associated with plasma membrane and partially localized in rafts. NIPSNAP proteins have been suggested to be important in vesicular transport. At least two isoforms of NIPSNAP3A are known to exist. NIPSNAP3A antibody is predicted to not cross-react with any other members of the NIPSNAP protein family. ...
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Story includes research items about: centronuclear myopathy; Duchenne, limb-girdle, and Emery-Dreifuss muscular dystrophies; and spinal muscular atrophy.Missing or reduced levels of an enzyme known as neuronal nitric oxide synthase (nNOS) at its normal location on muscle-fiber membranes prevents blood vessels that supply active muscles from relaxing normally, leading to exercise-associated fatigue, says a team of researchers from the University of Iowa in Iowa City, the University of Michigan in Ann Arbor, and the University of Washington School of Medicine in Seattle.. Read More ...
Crimson blood cells from mink ( em Mustela vison /em ) were characterized regarding their electrolyte content material and their cell membranes regarding enzymatic activity for cation transport. an inside-negative membrane potential in mink erythrocytes. Regardless of a steep calcium mineral gradient over the crimson cell membrane, neither a calmodulin-activated Ca2+-ATPase activity nor an ATP-activated Ca2+-pNPPase activity had been detectable in the plasma membrane small percentage. The origin of the supposed principal Ca2+ gradient for sustaining of osmotic stability thus appears uncertain. strong course=kwd-title Keywords: erythrocytes, plasma, electrolytes, crimson cell, mink crimson cells, Na+,K+-ATPase, membrane potential, osmotic stability, PM-CaATPase Launch The plasma membrane-embedded (Na++K+)-turned on ATPase (Na,K-ATPase, EC 3.6.1.37) of mammalian cells is normally supposed to have got an essential function in counterbalancing passive ionic leakages and oncotic pushes from ...
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1. Homogenates were prepared from sphaeroplasts of aerobically grown glucose-de-repressed Saccharomyces carlsbergensis and the distributions of marker enzymes were investigated after differential centrifugation. Cytochrome c oxidase and cytochrome c were sedimented almost completely at 105g-min, and this fraction also contained 37% of the catalase, 27% of the acid p-nitrophenyl phosphatase, 53 and 54% respectively of the NADH- and NADPH-cytochrome c oxidoreductases. 2. Zonal centrifugation indicated complex density distributions of the sedimentable portions of these enzymes and of adenosine triphosphatases and suggested the presence of two mitochondrial populations, as well as a bimodal distribution of peroxisomes and heterogeneity of the acid p-nitrophenyl phosphatase-containing particles. 3. Several different adenosine triphosphatases were distinguished in a post-mitochondrial supernatant that contained no mitochondrial fragments; these enzymes varied in their sensitivities to oligomycin and ...
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BioAssay record AID 88393 submitted by ChEMBL: In vitro inhibition of Hematopoietic protein-tyrosine phosphatase using p-nitrophenyl phosphate as substrate..
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Quite right, Donnachadh. And in those days everyone smelt like a decomposing kipper and I doubt very much if LOreal shifted a whole lot of product either. Why do these soap-dodging greenies want to take us back to being neanderthal cave dwellers? I wonder if they really fancy the whole authentic experience. How about a world with no antibiotics or anaesthetics? There you are, in your cave, nibbling on an old bit of tofu, shivering in the freezing cold and youve got a raging toothache and a streptococcal infection that could scare the shit out of vancomycin. As you lay there in absolute agony, all you can smell is the fetid armpits of Ugg sat next to you. Is that the sort of world these people really want us to live in? It cant be long before they call for mandatory limits on the number of showers we can take ...
Distinguish between (i) Peptic and oxyntic cells (ii) Lipases and peptidases (///) Villi and microvilli (iv) Sucrose and maltose (v) Extracellular and intracellular digestion.
Volvo V40 T5 now with R-Design exterior - RM180,888 - The facelifted Volvo V40 T5 now comes with the R-Design exterior package, replacing the Inscription kit that was available when the model was launched in... ...
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They fused the sequence for Bt toxin Cry1Ac with that of the nontoxic B-chain subunit of ricin (RB) in a recombinant plasmid. RB is a leptin that binds with galactose as well as N-acetylgalactosamine residues with high affinity, the latter of which are key components of Bt toxin-binding receptors. Then embryonic callus from mature maize seeds were bombarded with this BtRB fusion. The researchers tested their fusion toxin against stem borer Chilo suppressalis, a pest normally susceptible to Cry1Ac, and found that maize producing low levels of BtRB killed 75 per cent of larvae, compared with 17 per cent in Bt-only plants. Similar trials with the cotton leaf worm Spodoptera littoralis, which is resistant to Bt delta endotoxins, showed that after 4 days, nearly 78 per cent of larvae died on BtRB maize, compared with less than 20 per cent on Bt-only or nontransformed maize. In the leafhopper Cicadulina mbila, which like other homopterans was ordinarily not affected by Bt toxins, 95 per cent of ...
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Bullfrog tadpole stomachs of various metamorphic stages were examined to determine the fine-structural development of oxyntic cells and to correlate observed morphological development with the capacity to secrete HCl. It was found that in vitro tadpole stomachs can consistently be stimulated to secrete acid by stage XXIV of metamorphosis, when tail reabsorption is nearly complete. Concomitant with the appearance of HCl secretion, identifiable oxyntic cells were found in the gastric glands.. Prior to stage XXIV (stages XXI and XXII) the majority of cells present in the developing gastric glands exhibit features of cytological organization characteristic of undifferentiated cells: large nuclei, relatively scantry cytoplasm, and numerous ribosomal particles within the cytoplasmic matrix. The newly differentiated oxyntic cells of stage XXIV tadpole stomachs are recognizable by the accumulation of tubular members of the smooth-surfaced endoplasmic reticulum in the apical portion of the cells. These ...
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AP is purified from calf intestine by affinity chromatography. AP is an important label in enzyme immuno assays for substrates like pNPP and BCIP
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Thioglycosides have proved to be useful, enzymatically stable analogs of glycosides for structural and mechanistic studies and their synthesis is considerably simplified through the use of thioglycoligases. As part of an investigation into the use of thioglycosides as potential pharmacological chaperones, and as components of glycoproteins and glycolipids, the syntheses of p-nitrophenyl 3-thio-β-D-galactopyranoside, phenyl 1,4-dithio-β-D-glucopyranoside, p-nitrophenyl 4-thio-β-D-mannopyranoside and p-nitrophenyl 2-acetamido-2-deoxy-4-thio-β-D-mannopyranoside are described.
Alkaline phosphatase induces the hydrolysis of p-nitrophenyl phosphate in an alkaline solution, and forms a yellow colored complex whose intensity, measured at 405 nm, is directly proportional to the concentration of ALP in the sample.. ...
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TY - JOUR. T1 - Cloning, Expression, and Catalytic Mechanism of the Low Molecular Weight Phosphotyrosyl Protein Phosphatase from Bovine Heart. AU - Wo, Yu Yuan P.. AU - Zhou, Ming Ming. AU - Stevis, Panayiotis. AU - Davis, June P.. AU - Zhang, Zhong Yin. AU - Van Etten, Robert L.. PY - 1992/2/1. Y1 - 1992/2/1. N2 - The first representative of a group of mammalian, low molecular weight phosphotyrosyl protein phosphatases was cloned, sequenced and expressed in Escherichia coli. Using a 61-mer oligonucleotide probe based on the amino acid sequence of the purified enzyme, several overlapping cDNA clones were isolated from a bovine heart cDNA library. A full-length clone was obtained consisting of a 27-bp 5ʹ3ʹ noncoding region, an open reading frame encoding the expected 157 amino acid protein, and an extensive 3 nontranslated sequence. The identification of the clone as full length was consistent with results obtained in mRNA blotting experiments using poly(A)+ mRNA from bovine heart. The coding ...
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4-beta-glucosidase MeSH D08.811.277.450.420.200.600 - glucan endo-1,3-beta-d-glucosidase MeSH D08.811.277.450.420.375 - glucan ... map kinase kinase 4 MeSH D08.811.913.696.620.682.700.565.500 - map kinase kinase 5 MeSH D08.811.913.696.620.682.700.565.600 - ... map kinase kinase 4 MeSH D08.811.913.696.620.682.725.200.500 - map kinase kinase 5 MeSH D08.811.913.696.620.682.725.200.600 - ... type 4 MeSH D08.811.913.696.620.682.725.400.050 - proto-oncogene proteins c-kit MeSH D08.811.913.696.620.682.725.400.075 - ...
EC 3.2.1.91: cellulose 1,4-β-cellobiosidase (non-reducing end) EC 3.2.1.92: peptidoglycan β-N-acetylmuramidase EC 3.2.1.93: α,α ... 4-β-xylosidase EC 3.2.1.38: β-D-fucosidase EC 3.2.1.39: glucan endo-1,3-β-D-glucosidase EC 3.2.1.40: α-L-rhamnosidase EC 3.2. ... 4-α-glucosidase EC 3.2.1.4: cellulase EC 3.2.1.5: deleted EC 3.2.1.6: endo-1,3(4)-β-glucanase EC 3.2.1.7: inulinase EC 3.2.1.8 ... 4-β-xylanase EC 3.2.1.137: mannan exo-1,2-1,6-α-mannosidase EC 3.2.1.138: Now EC 4.2.2.15, anhydrosialidase EC 3.2.1.139: α- ...
Other names in common use include nitrophenyl phosphatase, p-nitrophenylphosphatase, para-nitrophenyl phosphatase, K-pNPPase, ... Attias J, Bonnet JL (1972). "A specific alkaline p-nitrophenylphosphatase activity from baker's yeast". Biochim. Biophys. Acta ... Attias J, Durand H (1973). "Further characterization of a specific p-nitrophenylphosphatase from baker's yeast". Biochim. ... NPPase, PNPPase, Ecto-p-nitrophenyl phosphatase, and p-nitrophenylphosphate phosphohydrolase. This enzyme participates in γ- ...
NIPSNAP14-Nitrophenylphosphatase Domain and Non-Neuronal SNAP25-Like Protein Homolog 1; Numb: Drosophila mutation that removes ... 1773 (4): 565-76. doi:10.1016/j.bbamcr.2007.01.001. PMID 17292493. Zhang SS, Xie X, Wen J, Luo KJ, Liu QW, Yang H, et al. ( ... 78 (4): 274-82. doi:10.1002/mrd.21303. PMID 21400627. S2CID 21140465. De Clercq K, Van den Eynde C, Hennes A, Van Bree R, Voets ... 57 (4): 427-50. doi:10.1124/pr.57.4.6. PMID 16382100. S2CID 17936350. Müller D, Hoenderop JG, Merkx GF, van Os CH, Bindels RJ ( ...
4-nitrophenylphosphatase (substance) {112042003 , SNOMED-CT } 6-phytase (substance) {69306002 , SNOMED-CT } Acetyl-coenzyme A ... Guanidinodeoxy-scyllo-inositol-4-phosphatase (substance) {34575009 , SNOMED-CT } Histidinol-phosphatase (substance) {14655001 ...
4)-beta-glucanase (substance) {33404002 , SNOMED-CT } Endo-1,4-beta-xylanase (substance) {77200000 , SNOMED-CT } Endo-alpha- ... 4-lactonase (substance) {77610004 , SNOMED-CT } 1,6-alpha-L-fucosidase (substance) {130551001 , SNOMED-CT } 11-cis-retinyl- ... 4-beta-glucosidase (substance) {60577003 , SNOMED-CT } Glucan 1,6-alpha-glucosidase (substance) {89224009 , SNOMED-CT } Glucan ... 4-beta-xylosidase (substance) {8368008 , SNOMED-CT } Xylan alpha-1,2-glucuronosidase (substance) {130555005 , SNOMED-CT } Xylan ...
Other names in common use include nitrophenyl phosphatase, p-nitrophenylphosphatase, para-nitrophenyl phosphatase, K-pNPPase, ... Attias J, Bonnet JL (1972). "A specific alkaline p-nitrophenylphosphatase activity from bakers yeast". Biochim. Biophys. Acta ... Attias J, Durand H (1973). "Further characterization of a specific p-nitrophenylphosphatase from bakers yeast". Biochim. ... NPPase, PNPPase, Ecto-p-nitrophenyl phosphatase, and p-nitrophenylphosphate phosphohydrolase. This enzyme participates in γ- ...
Dive into the research topics of Mechanistic insights into the manganese-dependent phosphodiesterase activity of yeast Dbr1 with bis-pnitrophenylphosphate and branched RNA substrates. Together they form a unique fingerprint. ...
4-NITROPHENYLPHOSPHATASE-RXN *Category: annotation *source: saccharina_japonica_genome; tool: pathwaytools; comment: n.a ...
4-nitrophenylphosphatase [Source:PomBase;Acc:SPBC15D4.15]. 0.02. OF. YDL236W. No alias. Conserved phosphatase acting as a ... Description : 4-nitrophenyl phosphatase;HAD-hyrolase-like;Haloacid dehalogenase-like hydrolase. Gene families : OG_03_0001149 ( ...
... "p-nitrophenyl phosphatase,4-nitrophenylphosphatase, " YHR030C -6.466319 INESSENTIAL SLT2 "serine threonine MAP kinase, protein ... 4-dienoyl-CoA reductase (NADPH), peroxisomal matrix" YBR083W 4.939094 INESSENTIAL TEC1 "transcription factor of the TEA ATTS ... imidazole-4-carboxamide isomerase, cell" YKR026C 2.47721 INESSENTIAL GCN3 34 KD alpha subunit of eIF2B YPL170W 2.474979 ... 4-glucan-6-(1,4-glucano)-transferase,1,4-alpha-glucan branching enzyme," YMR129W 1.973708 INESSENTIAL POM152 "Nuclear pore ...
... caspase-4 3.4.22.58 caspase-5 3.4.22.59 caspase-6 3.4.22.60 caspase-7 3.4.22.61 caspase-8 3.4.22.62 caspase-9 3.4.22.63 caspase ... 4-alpha-glucosidase 3.2.1.4 cellulase 3.2.1.5 Deleted entry 3.2.1.6 endo-1,3(4)-beta-glucanase 3.2.1.7 inulinase 3.2.1.8 endo-1 ... 4-beta-xylanase 3.2.1.9 Deleted entry 3.2.1.10 oligo-1,6-glucosidase 3.2.1.11 dextranase 3.2.1.12 Transferred entry: 3.2.1.54 ... 4-beta-xylosidase 3.2.1.38 beta-D-fucosidase 3.2.1.39 glucan endo-1,3-beta-D-glucosidase 3.2.1.40 alpha-L-rhamnosidase 3.2.1.41 ...
This graph shows the total number of publications written about "Phosphoric Monoester Hydrolases" by people in Harvard Catalyst Profiles by year, and whether "Phosphoric Monoester Hydrolases" was a major or minor topic of these publication ...
4-nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1 (C. elegans). 0.025. ... solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 4. 0.029. ...
The protein complex also contains glutamate dehydrogenase (GDH1), 4-nitrophenylphosphatase domain and non-neuronal SNAP25-like ... The protein complex also contains glutamate dehydrogenase (GDH1), 4-nitrophenylphosphatase domain and non-neuronal SNAP25-like ... The protein complex also contains glutamate dehydrogenase (GDH1), 4-nitrophenylphosphatase domain and non-neuronal SNAP25-like ... The protein complex also contains glutamate dehydrogenase (GDH1), 4-nitrophenylphosphatase domain and non-neuronal SNAP25-like ...
94; was PHOSPHOMONOESTERASES 1975-93; PHOSPHORIC MONOESTER HYDROLASES was see PHOSPHOMONOESTERASES 1975-93; PHOSPHATASES was heading 1963-93; PHOSPHOHYDROLASES was see PHOSPHATASES 1963- ...
... This is the entry page of medical terms started with 4. Please click to navigate more by the ...
Calcineurin-like properties of p-nitrophenylphosphatase isolated from the nuclear particulate fraction of the acellular slime ... Watts, J. M., Singh, J., Schoppe, C. H. & Johnson, A. J., Sep 2012, In: Clinical Neuroradiology. 22, 3, p. 253-256 4 p.. ... Dominiczak, A. F., Webb, R. C. & Bohr, D. F., Dec 1 1991, In: Journal of Vascular Medicine and Biology. 3, 4, p. 178-180 3 p.. ... Weissman, A. S., Patel, V. S. & Davis, L. S., Apr 2021, In: American Journal of Medicine. 134, 4, p. 474-476 3 p.. Research ...
Partial characterization of a nuclear p-nitrophenyl phosphatase from the acellular slime mold Physarum polycephalum. Marrero, M ... Clark, L. L. & Caughman, W. F., Sep 1 1984, In: Operative dentistry. 9, 4, p. 136-142 7 p.. Research output: Contribution to ... Quinn, W. H. & Davidson, B., Oct 1984, In: Journal of Marital and Family Therapy. 10, 4, p. 393-398 6 p.. Research output: ... Sutherland, J. C. & Griffin, K. P., Sep 1984, In: Photochemistry and Photobiology. 40, 3, p. 391-394 4 p.. Research output: ...
1,4-alpha-glucan branching enzyme. 1,4-beta-D-xylan synthase. 1,4-lactonase. 1,5-anhydro-D-fructose reductase. 1,6-alpha-L- ... ADAMTS-4 endopeptidase. ADP deaminase. ADP--thymidine kinase. ADP-dependent medium-chain-acyl-CoA hydrolase. ADP-dependent ... 4,5-dioxygenase. 4-(2-carboxyphenyl)-2-oxobut-3-enoate aldolase. 4-(cytidine 5-diphospho)-2-C-methyl-D-erythritol kinase. 4-( ... 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase. 4-hydroxy-4-methyl-2- ...
... and that K+-activated p-nitrophenylphosphatase (the enzyme responsible for the dephosphorylation of NKA) has been localized to ... 4). In L. semifasciata, AB+ material was detected only at the apical margin of the cells comprising the distal tubules (early ... 4) and no evidence for the upregulation of the muco-synthesis pathway (no change in the domain of expression of PAS+ material ... Sample sizes, per treatment: N. c. clarkii (N=5) and N. fasciata (N=5, 5, 4 and 3 for control, 0, 50 and 100% SW, respectively ...
p-nitrophenyl phosphatase 27, 274, 282. VNG0916G. ark. adaptive-response sensory-kinase 27, 274, 282. ... POSITION A C G T 1 0.0 0.0 0.0 1.0 2 1.0 0.0 0.0 0.0 3 1.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0 5 0.0 0.0 1.0 0.0 6 0.0 0.0 1.0 0.0 7 ... POSITION A C G T 1 0.0 1.0 0.0 0.0 2 0.2 0.6 0.0 0.2 3 0.0 0.2 0.6 0.2 4 0.0 0.0 1.0 0.0 5 0.0 0.0 0.0 1.0 6 0.0 0.0 0.0 1.0 7 ... POSITION A C G T 1 0.0 0.0 0.0 1.0 2 1.0 0.0 0.0 0.0 3 1.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0 5 0.0 0.0 1.0 0.0 6 0.0 0.0 1.0 0.0 7 ...
Calcineurin-like properties of p-nitrophenylphosphatase isolated from the nuclear particulate fraction of the acellular slime ... Huisman, T. H. J., Chen, S. S., Nakatsuji, T. & Kutlar, F., 1985, In: Hemoglobin. 9, 4, p. 393-398 6 p.. Research output: ... Pashley, E. L., Birdsong, N. L., Bowman, K. & Pashley, D. H., Dec 1985, In: Journal of endodontics. 11, 12, p. 525-528 4 p.. ... Goldenring, J. R., Otis, L. C., Yu, R. K. & DeLorenzo, R. J., Apr 1985, In: Journal of Neurochemistry. 44, 4, p. 1229-1234 6 p. ...
In this study, a probable p-nitrophenyl phosphatase was cloned from Archaeoglobus fulgidus genome, expressed in E. coli BL21( ... Guo Z, Wang F, Shen T, Huang J, Wang Y, Ji C (2014) Crystal structure of thermostable p-nitrophenylphosphatase from Bacillus ... Kaneko Y, Toh-E A, Banno I, Oshima Y (1989) Molecular characterization of a specific p-nitrophenylphosphatase gene, PHO13, and ... Moreover, the deduced amino-acid sequence of this phosphatase has modest identities with three identified p-nitrophenyl phosphatase ...
1100 in BB and created in p-nitrophenyl phosphatase disodium substrate remedy (pNPP; Sigma, St Louis, MO, USA). The absorbance ... Posted on June 4, 2017 , Comments Off on Although a cure for HCV is over the near horizon, emerging ... 4]. Among all genotypes, theres as much as 31C33% nucleotide variety [4]. Different genotypes of HCV have already been proven ...
K-Dependent p-Nitrophenylphosphatase K-p NPPase Nitrophenyl Phosphatase p-NPPase p-Nitrophenylphosphatase para-Nitrophenyl ... Nitrophenyl Phosphatase Broader Concept UI. M0014914. Registry Number. 0. Terms. Nitrophenyl Phosphatase Preferred Term Term UI ... K-Dependent p-Nitrophenylphosphatase Term UI T028571. Date11/24/1982. LexicalTag NON. ThesaurusID UNK (19XX). ... para-Nitrophenyl Phosphatase Term UI T028578. Date10/14/1983. LexicalTag NON. ThesaurusID UNK (19XX). ...
K-Dependent p-Nitrophenylphosphatase K-p NPPase Nitrophenyl Phosphatase p-NPPase p-Nitrophenylphosphatase para-Nitrophenyl ... Nitrophenyl Phosphatase Broader Concept UI. M0014914. Registry Number. 0. Terms. Nitrophenyl Phosphatase Preferred Term Term UI ... K-Dependent p-Nitrophenylphosphatase Term UI T028571. Date11/24/1982. LexicalTag NON. ThesaurusID UNK (19XX). ... para-Nitrophenyl Phosphatase Term UI T028578. Date10/14/1983. LexicalTag NON. ThesaurusID UNK (19XX). ...
27 N0000170401 D-Ala(2),MePhe(4),Met(0)-ol-enkephalin N0000168380 D-Alanine Transaminase N0000167841 D-Amino-Acid Oxidase ... 4,4-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide N0000166410 Benzene N0000007525 Benzene Derivatives ... 4,5-Tetrahydro-8-chloro-3-methyl-5-phenyl-1H-3-benzazepin-7-ol N0000179007 1 alpha-hydroxyergocalciferol N0000179008 1,1,1- ... 4-alpha-Glucosidase N0000167706 Glucan 1,4-beta-Glucosidase N0000167709 Glucan Endo-1,3-beta-D-Glucosidase N0000007623 Glucans ...
4-nitrophenylphosphatase domain and non-neuronal SNAP25-like protein homolog 1 (C. elegans). 0.010. ...
4-DINITROPHENOL DYES ACRIDINE ORANGE DYES ACRIFLAVINE DYES ALCIAN BLUE DYES AMARANTH DYES AMIDO BLACK DYES AMINACRINE DYES ... BLOOD PROTEINS COMPLEMENT 4 BLOOD PROTEINS COMPLEMENT 4A BLOOD PROTEINS COMPLEMENT 4B BLOOD PROTEINS COMPLEMENT 5 BLOOD ... 4)-GLY(5)- CENTRAL NERVOUS SYSTEM AGENTS ENKEPHALIN, D-PENICILLAMINE (2,5)- CENTRAL NERVOUS SYSTEM AGENTS EPHEDRINE CENTRAL ... NOXAE 4-NITROQUINOLINE-1-OXIDE NOXAE 6-AMINONICOTINAMIDE NOXAE 6-MERCAPTOPURINE NOXAE 7,8-DIHYDRO-7,8-DIHYDROXYBENZO(A)PYRENE ...
K+-stimulated p-nitrophenyl phosphatase is not a partial reaction of the gastric (H+ + K+)-transporting ATPase. Evidence ... Intact SEC were used to study the ouabain-sensitive Na+,K+-ATPase and K+-pNPPase (K+-stimulated p-nitrophenyl phosphatase) ... Half of the (Na+ + K+)-transporting-ATPase-associated K+-stimulated p-nitrophenyl phosphatase activity of gastric epithelial ... 4. The ouabain-sensitive ATPase activities were inactivated by N-ethylmaleimide but the insensitive ATPase activity was not. 5 ...
Ouyang B, Baxter CS, Lam HM, Yeramaneni S, Levin L, Haynes E, Ho SM. Hypomethylation of dual specificity phosphatase 22 promoter correlates with duration of service in firefighters and is inducible by low-dose benzo[a]pyrene. J Occup Environ Med. 2012 Jul; 54(7):774-80 ...
The following pages link to G11442.t1: View (previous 250 , next 250) (20 , 50 , 100 , 250 , 500) ...
ATPase and p-nitrophenyl phosphatase activities and phosphoenzyme formation. Reaction of the enzyme with Mal-pyrene, but not ... In contrast, only 4 out of 15 alpha-hemolytic bovine group G strains were able to bind fibronectin. The uptake of fibronectin ... 4. In the presence of ouabain, the maximum inhibition of Mg2+-dependent phosphorylation by Ca2+ was 50%. With saturating Pi, ... Both NGF and EGF are bound to PC12 cells in a competable form at 4 degrees C. At 37 degrees C both ligands are "sequestered," ...
... encoding an N-acetylglucosamine-1-phosphate transferase and a4-nitrophenylphosphatase, respectively. Five ORFs similar to known ... Yeast 2000 Mar;16(4):299-306. We report the complete sequence of two cosmids, SPBC19C7 (34815 bp insert, Accession No. AL023859 ...
In addition, they also reported that Hg2+ inhibited K+-p-nitrophenyl phosphatase (K+-PNPPase), the component enzyme catalyzing ... 4. 5. .. 4. ±. 5. .. 8. %. inhibition of the methylmercury effect). These authors also found that hydrogen peroxide as a vector ... 4, no. 6, pp. 1598-1605, 1990. View at: Google Scholar*W. E. Harris and W. L. Stahl, "Origin of the γ polypeptide of the Na+/K+ ... 4, pp. 329-336, 1986. View at: Google Scholar*S. Magour, "Studies on the inhibition of brain synaptosomal Na+/K+-ATPase by ...
On the ouabain-sensitive potassium activated p-nitrophenyl phosphatase activity of vascular muscle plasma membranes. Academic ... Properties of potassium activatedp-nitrophenyl phosphatase of plasma membranes isolated from rat stomach muscle Academic ... Actions of 4-chloro-3-ethyl phenol on internal Ca2+stores in vascular smooth muscle and endothelial cells Academic Article ... 4-Aryl-3-(hydroxyalkyl)quinolin-2-ones: Novel Maxi-K Channel Opening Relaxants of Corporal Smooth Muscle Targeted for Erectile ...
  • The enzyme 4-nitrophenylphosphatase (EC 3.1.3.41) catalyzes the reaction 4-nitrophenyl phosphate + H2O ⇌ {\displaystyle \rightleftharpoons } 4-nitrophenol + phosphate This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. (wikipedia.org)