A benzofuran derivative used as a protein reagent since the terminal N-NBD-protein conjugate possesses interesting fluorescence and spectral properties. It has also been used as a covalent inhibitor of both beef heart mitochondrial ATPase and bacterial ATPase.
Very toxic and complex pyrone derivatives from the fungus Calcarisporium arbuscula. They bind to and inhibit mitochondrial ATPase, thereby uncoupling oxidative phosphorylation. They are used as biochemical tools.
A sulfhydryl proteinase with cysteine at the active site from ficus latex. Preferential cleavage is at tyrosine and phenylalanine residues. EC 3.4.22.3.
Compounds that contain a BENZENE ring fused to a furan ring.
Multisubunit enzymes that reversibly synthesize ADENOSINE TRIPHOSPHATE. They are coupled to the transport of protons across a membrane.
A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.
The mitochondria of the myocardium.
2-Chloroadenosine. A metabolically stable analog of adenosine which acts as an adenosine receptor agonist. The compound has a potent effect on the peripheral and central nervous system.
A family of nonmetallic, generally electronegative, elements that form group 17 (formerly group VIIa) of the periodic table.
Compounds containing the -SH radical.
A centrally active muscarinic antagonist that has been used in the symptomatic treatment of PARKINSON DISEASE. Benztropine also inhibits the uptake of dopamine.
The rate dynamics in chemical or physical systems.
An essential amino acid. It is often added to animal feed.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
A greenish-yellow, diatomic gas that is a member of the halogen family of elements. It has the atomic symbol Cl, atomic number 17, and atomic weight 70.906. It is a powerful irritant that can cause fatal pulmonary edema. Chlorine is used in manufacturing, as a reagent in synthetic chemistry, for water purification, and in the production of chlorinated lime, which is used in fabric bleaching.
A group of 1,2-benzenediols that contain the general formula R-C6H5O2.
Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.
The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Drugs that block the transport of adrenergic transmitters into axon terminals or into storage vesicles within terminals. The tricyclic antidepressants (ANTIDEPRESSIVE AGENTS, TRICYCLIC) and amphetamines are among the therapeutically important drugs that may act via inhibition of adrenergic transport. Many of these drugs also block transport of serotonin.
Compounds that specifically inhibit the reuptake of serotonin in the brain.
The first highly specific serotonin uptake inhibitor. It is used as an antidepressant and often has a more acceptable side-effects profile than traditional antidepressants.
Mood-stimulating drugs used primarily in the treatment of affective disorders and related conditions. Several MONOAMINE OXIDASE INHIBITORS are useful as antidepressants apparently as a long-term consequence of their modulation of catecholamine levels. The tricyclic compounds useful as antidepressive agents (ANTIDEPRESSIVE AGENTS, TRICYCLIC) also appear to act through brain catecholamine systems. A third group (ANTIDEPRESSIVE AGENTS, SECOND-GENERATION) is a diverse group of drugs including some that act specifically on serotonergic systems.
A furancarbonitrile that is one of the SEROTONIN UPTAKE INHIBITORS used as an antidepressant. The drug is also effective in reducing ethanol uptake in alcoholics and is used in depressed patients who also suffer from tardive dyskinesia in preference to tricyclic antidepressants, which aggravate this condition.
A serotonin uptake inhibitor that is effective in the treatment of depression.

Specific and sensitive assay for alkaline and neutral ceramidases involving C12-NBD-ceramide. (1/347)

A fluorescent analogue of ceramide, C12-NBD-ceramide, was found to be hydrolyzed much faster than 14C-labeled ceramide by alkaline ceramidase from Pseudomonas aeruginosa and neutral ceramidase from mouse liver, while this substrate was relatively resistant to acid ceramidase from plasma of the horseshoe crab. The radioactive substrate was used more preferentially by the acid ceramidase. It should be noted that C6-NBD-ceramide, which is usually used for ceramidase assays, was hardly hydrolyzed by any of the enzymes examined, compared to C12-NBD-ceramide. For the alkaline and neutral enzymes, the Vmax and k (Vmax/Km) with C12-NBD-ceramide were much higher than those with 14C-ceramide. In contrast, for the acid enzyme these parameters with C12-NBD-ceramide were less than half those with the radioisotope-labeled substrate. It is noteworthy that the labeling of ceramide with NBD did not itself reduce the Km of the alkaline enzyme, but did that of the neutral enzyme. It was also found that C12-NBD-ceramide was preferentially hydrolyzed by the alkaline and neutral enzymes, but not the acid one, in several mammalian cell lines. This study clearly shows that the attachment of NBD, but not dansyl, increases the susceptibility of ceramide to alkaline and neutral enzyme, and decreases that to acid enzymes. Thus the use of this substrate provides a specific and sensitive assay for alkaline and neutral ceramidases.  (+info)

Maturation of the axonal plasma membrane requires upregulation of sphingomyelin synthesis and formation of protein-lipid complexes. (2/347)

Neuronal maturation is a gradual process; first axons and dendrites are established as distinct morphological entities; next the different intracellular organization of these processes occurs; and finally the specialized plasma membrane domains of these two compartments are formed. Only when this has been accomplished does proper neuronal function take place. In this work we present evidence that the correct distribution of a class of axonal membrane proteins requires a mechanism which involves formation of protein-lipid (sphingomyelin/cholesterol) detergent-insoluble complexes (DIGs). Using biochemistry and immunofluorescence microscopy we now show that in developing neurons the randomly distributed Thy-1 does not interact with lipids into DIGs (in fully developed neurons the formation of such complexes is essential for the correct axonal targeting of this protein). Using lipid mass spectrometry and thin layer chromatography we show that the DIG lipid missing in the developing neurons is sphingomyelin, but not cholesterol or glucosylceramide. Finally, by increasing the intracellular levels of sphingomyelin in the young neurons the formation of Thy-1/DIGs was induced and, consistent with a role in sorting, proper axonal distribution was facilitated. These results emphasize the role of sphingomyelin in axonal, and therefore, neuronal maturation.  (+info)

Saturable stimulation of fatty acid transport through model cytoplasm by soluble binding protein. (3/347)

To better define the role of soluble binding proteins in the cytoplasmic transport of amphipathic molecules, we measured the diffusional mobility of a fluorescent long-chain fatty acid, 12-N-methyl-(7-nitrobenz-2-oxa-1,3-diazol)aminostearate (NBD-stearate), through model cytoplasm as a function of soluble binding protein concentration. Diffusional mobilities were correlated with the partition of the fatty acid between membrane and protein binding sites. Cytoplasm was modeled as a dense suspension of liposomes, and albumin was used as a model binding protein. Albumin saturably increased NBD-stearate mobility through the membrane suspension approximately eightfold. Fatty acid mobility in the absence of albumin was identical to the mobility of the membrane vesicles (1.99 +/- 0.33 x 10(-8) cm(2)/s), whereas the mobility at saturating concentrations was identical to the mobility of albumin (1.65 +/- 0.12 x 10(-7) cm(2)/s). The protein concentration producing half-maximal stimulation of NBD-stearate diffusion (42.8 +/- 0.3 microM) was unexpectedly greater than that required to solubilize half of the NBD-stearate (17.9 +/- 3.0 microM). These results support a proposed mechanism for cytoplasmic transport of small amphipathic molecules in which aqueous diffusion of the protein-bound form of the molecule largely determines the transport rate. However, slow interchange of fatty acid between the binding protein and membranes also appears to influence the transport rate in this model system.  (+info)

Fluorescent phosphoinositide derivatives reveal specific binding of gelsolin and other actin regulatory proteins to mixed lipid bilayers. (4/347)

Fluorescent derivatives of phosphatidyl inositol (PtdIns)-(4,5)-P2 were synthesized and used to test the effects of the PtdIns-(4, 5)-P2-regulated proteins gelsolin, tau, cofilin, and profilin on labeled PtdIns-(4,5)-P2 that was either in micellar form or mixed with phosphatidylcholine (PtdCho) in bilayer vesicles. Gelsolin increased the fluorescence of 7-nitrobenz-2-oxa-1,3-diazole (NBD)- or pyrene-labeled PtdIns-(4,5)-P2 and NBD-PtdIns-(3,4,5)-P3. Cofilin and profilin produced no detectable change at equimolar ratios to PtdIns-(4,5)-P2, while tau decreased NBD-PtdIns-(4,5)-P2 fluorescence. Fluorescence enhancement by gelsolin of NBD-PtdIns-(4, 5)-P2 in mixed lipid vesicles depended on the mole fraction of PtdIns-(4,5)-P2 in the bilayer. Specific enhancement of 3% NBD-PtdIns-(4,5)-P2 : 97% PtdCho was much lower than that of 10% PtdIns-(4,5)-P2 : 90% PtdCho, but the enhancement of 3% NBD-PtdIns-(4,5)-P2 could be increased by addition of 7% unlabeled PtdIns-(4,5)-P2. The gelsolin-dependent increase in NBD-PtdIns-(4, 5)-P2 fluorescence was reversed by addition of Ca2+ or G-actin. Significant, but weaker, fluorescence enhancement was observed with the gelsolin N-terminal domain (residues 1-160) and a peptide comprised of gelsolin residues 150-169. Fluorescence energy transfer from gelsolin to pyrene-PtdIns-(4,5)-P2 was much stronger with intact gelsolin than the N-terminal region of gelsolin containing the PtdIns-(4,5)-P2 binding sites, suggesting that PtdIns-(4,5)-P2 may bind near a site formed by the juxtaposition of the N- and C-terminal domains of gelsolin.  (+info)

Rapid transbilayer movement of fluorescent phospholipid analogues in the plasma membrane of endocytosis-deficient yeast cells does not require the Drs2 protein. (5/347)

Evidence is presented that endocytosis-deficient Saccharomyces cerevisiae end4 yeast cells rapidly internalize the fluorescent phospholipid analogues 1-palmitoyl-2-{6-[7-nitro-2,1, 3-benzoxadiazol-4-yl(NBD)amino] caproyl}phosphatidylcholine (P-C6-NBD-PtdCho) and P-C6-NBD-phosphatidylserine (P-C6-NBD-PtdSer). Both analogues redistributed between the exoplasmic and cytoplasmic leaflet with a half-time of < 15 min at 0 degrees C. The plateau of internalized analogues was about 70%. Transbilayer movement is probably protein-mediated, as the flip-flop of both analogues was very slow in liposomes composed of plasma-membrane lipids. Rapid analogue internalization was not abolished on depletion of intracellular ATP by about 90%. For P-C6-NBD-PtdCho only was a moderate decrease in the plateau of internalized analogues of about 20% observed, while that of P-C6-NBD-PtdSer was not affected. The Drs2 protein plays only a minor role, if any, in the rapid transbilayer movement of analogues in S. cerevisiae end4 cells. In S. cerevisiae end4 Deltadrs2 cells harbouring both an end4 allele and a drs2 null allele, about 60% and 50% of P-C6-NBD-PtdCho and P-C6-NBD-PtdSer, respectively, became internalized within 15 min at 0 degrees C. The preferential orientation of P-C6-NBD-PtdSer to the cytoplasmic leaflet is in qualitative agreement with the sequestering of endogenous phosphatidylserine to the cytoplasmic leaflet, as assessed by binding of annexin V. Virtually no binding of annexin V to spheroplasts of the parent wild-type strain or the mutant strains was observed. Likewise, no difference in the exposure of endogenous aminophospholipids to the exoplasmic leaflet between these strains was found by labelling with trinitrobenzenesulfonic acid. Thus, lipid asymmetry, at least of aminophospholipids, was preserved in S. cerevisiae end4 cells independently of the presence of the Drs2 protein.  (+info)

Cytoplasmic transport of fatty acids in rat enterocytes: role of binding to fatty acid-binding protein. (6/347)

The intracellular movement of fatty acids is thought to be facilitated through codiffusion with fatty acid-binding protein (FABP). This facilitation may occur by decreasing binding to immobile membranes, leading to faster cytoplasmic diffusion. The aims of this study were to measure the intracellular transport of 12-N-methyl-(7-nitrobenzo-2-oxa-1,3-diazol)aminostearate (NBD-stearate) in villus rat enterocytes and to determine 1) the mechanism of its cytoplasmic transport and 2) if its transport rate correlated with the known variation of FABP binding capacity along the length of the small intestine. Two-dimensional laser photobleaching was used to measure the movement of a fluorescent fatty acid NBD-stearate in enterocytes isolated from different segments of rat intestine. The fraction of NBD-stearate found in the cytostol of enterocytes was determined by differential centrifugation. Cytoplasmic transport of NBD-stearate occurred solely by diffusion and not by convection. Diffusion was homogeneous (nondirectional), consistent with isotropic diffusion. The diffusion rate varied with location along the intestine, correlating with the local FABP concentration and measured cytosolic binding. We conclude that cytoplasmic proteins like FABP promote the intracellular transport of fatty acids by enhancing their diffusive flux. We suggest that facilitation is not specific for a particular cell type but occurs in a variety of cells that transport fatty acids and may contain different types of FABP.  (+info)

Brownian ratchets: molecular separations in lipid bilayers supported on patterned arrays. (7/347)

Brownian ratchets use a time-varying asymmetric potential that can be applied to separate diffusing particles or molecules. A new type of Brownian ratchet, a geometrical Brownian ratchet, has been realized. Charged, fluorescently labeled phospholipids in a two-dimensional fluid bilayer were driven in one direction by an electric field through a two-dimensional periodic array of asymmetric barriers to lateral diffusion fabricated from titanium oxide on silica. Diffusion spreads the phospholipid molecules in the orthogonal direction, and the asymmetric barriers rectify the Brownian motion, causing a directional transport of molecules. The geometrical ratchet can be used as a continuous molecular sieve to separate mixtures of membrane-associated molecules that differ in electrophoretic mobility and diffusion coefficient.  (+info)

Location of the catalytic nucleophile of phospholipase D of Streptomyces antibioticus in the C-terminal half domain. (8/347)

Phospholipase D (PLD) of Streptomyces antibioticus was labelled with fluorescent-labelled substrate, 1-hexanoyl-2-{6-[(7-nitro-2-1, 3-benzoxadiazol-4-yl)-amino]hexanoyl}-sn-glycero-3-phosphocholine, when it was incubated with the substrate and the reaction followed by SDS/PAGE. Mutant enzymes lacking the catalytic activity were not labelled under the same conditions, indicating that labelling of the PLD occurred as the result of its catalytic action. This confirmed that the labelled protein was the phosphatidyl PLD intermediate. PLDs contain two copies of the highly conserved catalytic HxKxxxxD (HKD) motif. Therefore, two protein fragments were separately prepared with recombinant strains of Escherichia coli. One of the fragments was the N-terminal half of the intact PLD containing one HKD motif, and the other was the C-terminal half with the other motif. An active enzyme was reconstructed from these two fragments, and therefore designated fragmentary PLD (fPLD). When fPLD was subjected to the labelling experiment, only the C-terminal half was labelled. Therefore, it was concluded that the catalytic nucleophile that bound directly to the phosphatidyl group of the substrate was located on the C-terminal half of PLD, and that the N-terminal half did not contain such a nucleophile.  (+info)

TY - JOUR. T1 - Selective N-terminal fluorescent labeling of proteins using 4-chloro-7-nitrobenzofurazan. T2 - A method to distinguish protein N-terminal acetylation. AU - Bernal-Perez, Lina F.. AU - Prokai, Laszlo. AU - Ryu, Youngha. PY - 2012/9/1. Y1 - 2012/9/1. N2 - A fluorogenic derivatization method was developed to distinguish the protein N-terminal acetylation status. The unacetylated protein selectively reacted with 4-chloro-7-nitrobenzofurazan (NBD-Cl) at neutral pH to provide high fluorescence. In contrast, the protein with N-terminal acetylation was essentially nonfluorescent under the same conditions despite the presence of many internal lysine residues. Fluorescence of the NBD-labeled protein was very stable, and only micromolar concentrations of proteins were required for reliable detection. This method also provides a general and practical way to quantify proteins when their N-terminal amino group is available.. AB - A fluorogenic derivatization method was developed to distinguish ...
TY - JOUR. T1 - Fluorimetric determination of D- and L-lactate derivatized with 4-(N,N-dimethylaminosulfonyl)-7-piperazino-2,1,3-benzoxadiazole (DBD-PZ) by high-performance liquid chromatography. AU - Fukushima, Takeshi. AU - Adachi, Sinya. AU - Ichihara, Hideaki. AU - Al-Kindy, Salma. AU - Imai, Kazuhiro. PY - 1999. Y1 - 1999. N2 - A highly sensitive method, based on fluorescence derivatization with 4-N,N-dimethylaminosulfonyl-7-piperazino-2,1,3-benzoxadiazole (DBD-PZ), was developed for the determination of D- and L-lactic acid. High-performance liquid chromatographic separation of the lactic acid derivative was achieved using an octadecylsilica column followed by enantiomeric separation on a phenylcarbamoylated β-cyclodextrin chiral column. The separation factor for D,L-lactic acid derivatives was 1.30 using MeOH/H2O (80/20) as a mobile phase, and the detection limits were approximately 360 and 300 fmol on column for D- and L-lactic acid derivative, respectively. The proposed method was ...
Learn more about 4-chloro-7-nitrobenzofurazan. We enable science by offering product choice, services, process excellence and our people make it happen.
Using brush-border membrane vesicles isolated from calf kidney cortex the effect of tyrosine-reactive reagents on sodium-dependent D-glucose transport was investigated. Treatment of the membranes for 60 min with NBD-Cl (7-chloro-4-nitrobenzo-2-oxa-1,3-diazole), N-acetylimidazole or tetranitromethane decreased D-glucose uptake 50, 70 and 40%, respectively. Tracer exchange experiments revealed that the inhibition of transport is due to a direct modification of the sodium-D-glucose cotransport system. The modification by NBD-Cl decreases the apparent Vmax of the transport system with respect to its interaction with sodium. In addition, the rate of inactivation of the transport system by NBD-Cl is reduced in the presence of high concentrations of sodium. The results indicate that tyrosine residues play an essential role in sodium-D-glucose cotransport and are probably involved in the binding and/or transport of sodium by the sodium-D-glucose cotransport system.
Cholesterol (Chol) is a crucial component of cellular membranes, but knowledge of its intracellular dynamics is scarce. Thus, it is of utmost interest to develop tools for visualization of Chol organization and dynamics in cells and tissues. For this purpose, many studies make use of fluorescently labeled Chol analogs. Unfortunately, the introduction of the label may influence the characteristics of the analog, such as its localization, interaction, and trafficking in cells; hence, it is important to get knowledge of such bias. In this report, we compared different fluorescent lipid analogs for their performance in cellular assays: 1) plasma membrane incorporation, specifically the preference for more ordered membrane environments in phase-separated giant unilamellar vesicles and giant plasma membrane vesicles; 2) cellular trafficking, specifically subcellular localization in Niemann-Pick type C disease cells; and 3) applicability in fluorescence correlation spectroscopy (FCS)-based and super-resolution
D-Glucose is one of the most important energy sources for the survival of various organisms, from E. colito mammals. For live-cell monitoring of glucose uptake at the single-cell level, a fl uorescent D-glucose derivative 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4- yl)amino]-2-deoxy-D-glucose [2-NBDG], which we developed, has been widely used invarious research fi elds. For the last ten years, however, researchers have awaited an optical control substance forevaluating the extent of non-specifi c adsorption of 2-NBDG upon plasma membrane and/or the rate of unhealthy2-NBDG uptake through partially( or transiently) damaged membrane. Here we introduce a fluorescent L-glucose derivative, 2-[N(- 7-Nitrobenz-2-oxa- 1,3-diazol-4-yl)amino]- 2-deoxy-Lglucose[2-NBDLG]. L-Glucosamine is a key intermediate toward the synthesis of 2-NBDLG, but not commerciallyavailable. Although a few papers on the synthesis of L-glucosamine have been reported, a new synthetic method ofL-glucosamine should be absolutely
I am planning to label some amino acids with NBD-F (4-fluoro-7-nitrobenzofurazan). In some papers Ive read, these derivatives are stated to be very stable. In other papers, they are said to be stable for up to two days when stored in the dark in a refrigerator. Does anyone know what the best storage conditions are? Although this is just a guess, I would think that evaporating the samples to dryness under nitrogen (or with a SpeedVac), and then storing them in a freezer, would increase their storage life. Of course, this assumes that they are more stable when dry, which may not be the case. Anyone know what conditions are most favorable for the preservation/storage of NBD-labeled amino acids? Phil Calvert . -- ------------------------------------ !-----------Phil Calvert-----------! !---calvert.NoSpam at eos.ncsu.edu----! ------------------------------------ NOTE: If present, the phrase .NoSpam must be removed from my return address before sending your reply ...
This study was undertaken to determine whether 4-benzylamino-7-nitrobenz-2-oxa-l,3-diazole (BBD) generates the first excited singlet state of molecular oxygen in liquid solution. The problem was first approached using the ...
Item #: SCP-827. Object Class: Safe. Special Containment Procedures: Site 827 has been established at the location of SCP-827s discovery. For the purposes of the Foundation, SCP-827 has been outfitted with a specialized cell reactor that allows for introduction of samples and removal of their products. Personnel actively interacting with SCP-827 are to wear full Level-C or higher Hazmat gear.. Samples introduced into SCP-827 require approval of project director. Samples from only one individual at a time are to be introduced to ensure there is no genetic cross-contamination. All samples are to be screened for genetic chimerism. In the event that more than one distinct genetic sample is introduced to SCP-827, the sample is to be removed using procedure 827-Hari and incinerated.. Tissue from the central nervous system is not to be used in SCP-827 tests following Incident 827-██.. Description: SCP-827 is a semi-solid mass of biologically active human stem cells. SCP-827 is capable of ...
Learn more about 4-chloro-6-trifluoromethyl-quinoline. We enable science by offering product choice, services, process excellence and our people make it happen.
Fluorescent glucose biosensors are devices that measure the concentration of glucose in diabetic patients by means of sensitive protein that relays the concentration by means of fluorescence, an alternative to amperometric sension of glucose. No device has yet entered the medical market, but, due to the prevalence of diabetes, it is the prime drive in the construction of fluorescent biosensors. Keeping glucose levels in check is crucial to minimize the onset of the damage caused by diabetes. As a consequence, in conjunction with insulin administrations, the prime requirement for diabetic patients is to regularly monitor their blood glucose levels. The monitoring systems currently in general use have the drawback of below optimal number of readings, due to their reliance on a drop of fresh blood. Some continuous glucose monitors are commercially available, but suffer from the severe drawback of a short working life of the probe. The majority of these work amperometrically. As a result, there is ...
Changes in the spectral properties of plasma membrane lipid analog during the first seconds of endocytosis in living cells, Biophys. , 72, 32-50, 1997. , A novel fluorescent ceramide analog for studying membrane traffic in animal cells; accumulation at the Golgi apparatus results in altered spectral properties of the sphingoid precursor, J. , 113, 1267-1279, 1991. 7 mM), a fluorescent probe of the Golgi apparatus. A leopard-skin appearance of fluorochrome-loaded Golgi sacks is obtained. Similar fluorescence images may be recorded when the cell is treated with fluorescent cytotoxic agents (see Chapter 3). Doctoral dissertation, Carnegie-Mellon University, Pittsburgh, PA, 1990. fm Page 14 Tuesday, November 18, 2003 8:40 AM 14 Atlas of Cell Fluorescence FIGURE 12 Lysosomes with lysososomotropic agent quinacrine. Color image; microphotograph. fm Page 15 Tuesday, November 18, 2003 8:40 AM Vital Fluorescence Probes of Cell Organelles FIGURE 13 Quinacrine imaging of lysosomes. SP micrograph. fm Page 16 ...
Figure 3. Sphingomyelin trafficking and metabolism measured in KB-3-1 and KB-CP.5 cells. A and B, NBD-sphingomyelin labeling of KB-3-1 (A) and KB-CP.5 (B) cells. The cells were labeled with NBD-sphingomyelin at 37° for 1 minute, chased for 30 minutes to achieve steady-state distribution, back exchanged on ice to remove excess cell surface label, and then imaged using wide-field microscopy. Bar, 10 μm. C and D, KB-CP.5 cells double labeled with Alexa 546-transferrin (C) and NBD-sphingomyelin (D). Negligible colocalization is observed. Bar, 10 μm. E, ratio of NBD-ceramide to NBD-sphingomyelin in KB-3-1 cells (black columns) and KB-CP.5 cells (white columns) after excess NBD-sphingomyelin on the plasma membrane has been removed by back exchange. The NBD-lipid ratio thus represents primarily the NBD-lipid proportions in the ERC (because most of the NBD-sphingomyelin from the plasma membranes is removed by the back-exchange procedure). The protocol used for the NBD-lipid ratio assay is explained ...
The sensor was successfully deployed in all five patients and did not interfere with clinical care, blood pressure monitoring or sampling. One patient suffered a cardiopulmonary arrest; the sensor functioned successfully during resuscitation and urgent return to the operating room. One hundred and twenty reference samples ranging from 5.9 to 13.4 mmol/l were collected; 107/120 (89.2%) of GluCath measurements met ISO 15197 criteria (within ±20% of reference when BG ,4.2 mmol/l; Figure 1). In Subject 1 the sensor was inadvertently retracted into the arterial catheter during the study, leading to measurement error from arterial flush solution contamination. In a sensitivity analysis excluding this patient, 89/95 (93.7%) of measurements met ISO 15197 with a mean absolute relative difference of 9.4 ...
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2-NBD-Glucose (186689-07-6) is a fluorescent glucose uptake probe. May be used to monitor glucose uptake in live cells and as a cell viability indi ...
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Item #: SCP-1816. Object Class: Safe. Special Containment Procedures: SCP-1816 is confined in an underground experimental room measuring 10m x 10m x 5m. The room is climate-controlled and equipped with sunlight-simulating lamps. Access to SCP-1816 is forbidden to all female personnel, unless approved by Dr. Coulloudon. The maintenance of SCP-1816, including pruning, fertilization and re-potting, is to be conducted by Professor H. Pak.. Description: SCP-1816 is a penjing containing a live specimen of an unidentified tree. The object is approximately 40 cm high.. SCP-1816 will only affect pregnant mammals. When a subject at an early stage of pregnancy is left in the same room as SCP-1816, the fetus may become an instance of SCP-1816-1. The distance and time of exposure required to cause this effect depends on the size and gestation period of the individual. In the case of murine test subjects, an exposure of 3 minutes/day within 5 meters was found to be sufficient. The requirements for larger ...
Begin Log,. Dr. Hayfield: How long have you been connected to SCP-1884-B?. SCP-1884-A: As long as I can remember. Wherever I have been, Luana has been there with me, even if only in my mind.. Dr. Hayfield: Where did SCP-1884-B come from?. SCP-1884-A: When I was still very young, I asked my mother the same question. She would not tell me. She said she did not want to frighten me.. Dr. Hayfield: Have your blindness and physical abnormalities been present since birth?. SCP-1884-A: Yes. Luana has always been my eyes. She feels the ground so I can walk. She helps me hold things. In my old age, there have been times she has carried me. I am very grateful to her.. Dr. Hayfield: What were the events that led to the incident at the hotel?. SCP-1884-A: That may take some time to explain.. Dr. Hayfield: Thats perfectly fine. Please proceed.. SCP-1884-A: When I was eight years old, men came to our house asking to buy me and Luana. My parents were upset. They always tried to hide us and keep us a secret, ...
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ethyl 7-chloro-4-hydroxy-3-quinolinecarboxylate - chemical structural formula, chemical names, chemical properties, synthesis references
We have examined intracellular transport and metabolism of the fluorescent analogue of phosphatidylserine, 1-palmitoyl-2-(N-[12[(7-nitrobenz-2-oxa-1,3-diazole-4-yl)amino] dodecanoyl])-phosphatidylserine ([palmitoyl-C12-NBD]-PS) in cultured fibroblasts. When monolayer cultures were incubated with liposomes containing (palmitoyl-C12-NBD)-PS at 37 degrees C, fluorescent PS was transported to the Golgi apparatus. NBD-containing analogues of phosphatidylcholine, phosphatidylethanolamine (PE), or phosphatidic acid did not accumulate in the Golgi apparatus under the same experimental conditions. We suggest that the transport is not due to endocytosis, but is the result of incorporation and trans-bilayer movement of the (palmitoyl-C12-NBD)-PS at the plasma membrane followed by translocation of the lipid from plasma membrane to the Golgi apparatus via nonvesicular mechanisms. Uptake of fluorescent PS was inhibited by depletion of cellular ATP and was blocked by structural analogues of the lipid or by ...
Materials and cell culture. 1,2-Dioleoyl-sn-glycero-3-phosphocholine, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, N-hexanoyl-d-erythro-sphingosine (C6), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy polyethylene glycol-2000], N-octanoyl-sphingosine-1-[succinyl(methoxy polyethylene glycol-750)] (PEG(750)-C8), N-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-d-erythro-sphingosine (NBD-C6), and N,N-dimethyl-d-erythro-sphingosine (DMSph) were purchased from Avanti Polar Lipids (Alabaster, AL). N-hexanoyl-d-erythro-sphingosine [hexanoyl 6-3H] ([3H]C6) was obtained from ARC (St. Louis, MO). Primary antibodies to caveolin-1 and CD31 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA) and BD PharMingen (San Jose, CA), respectively. Horseradish peroxidase-conjugated goat anti-rabbit IgG and rhodamine red-X-conjugated goat anti-rat IgG secondary antibodies were obtained from Santa Cruz Biotechnology and Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA), ...
You are viewing an interactive 3D depiction of the molecule 2-({6-[(bromoacetyl)amino]hexanoyl}amino)-2-deoxy-d-glucose (C14H25BrN2O7) from the PQR.
The time course of interaction of caldesmon with actin may be monitored by fluorescence changes that occur upon the binding of 12-(N-methyl-N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl))-labeled caldesmon to actin or to acrylodan ...
The time course of interaction of caldesmon with actin may be monitored by fluorescence changes that occur upon the binding of 12-(N-methyl-N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl))-labeled caldesmon to actin or to acrylodan ...
Sigma-Aldrich offers abstracts and full-text articles by [Raiyan T Zaman, Hisanori Kosuge, Guillem Pratx, Colin Carpenter, Lei Xing, Michael V McConnell].
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C07D233/66-Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms ...
Name: 2-Chloro-4-nitrobenzenamine CA Name: Benzenamine,2-chloro-4-nitro- Molecular Structure: 2-Chloro-4-nitrobenzenamine,Benzenamine,2-chloro-4-nitro-,CAS 121-87-9,172.57,C6H5ClN2O2 2-Chloro-4-nitrobenzenamine,Benzenamine,2-chloro-4-nitro-,CAS 121-87-9,172.57,C6H5ClN2O2 Molecular Formula:C6H5ClN2O2 Molecular Weight: 172.57 CAS Registry Number: 121-87-9
Entry Interview for SCP-4409. Interviewer: Dr. Simon Crossley. ,Begin Log,. Dr. Crossley: Good afternoon, SCP-4409, and welcome to Site-66. Im Dr. Crossley, and Ill be conducting both your initial interview and medical exam. Lets see, my boss gave me a list of questions Im supposed to ask you -. SCP-4409: Is the first one why do I look like an elephant?. Dr. Crossley: Ah, its worded a bit more generically than that, but yes. How long has your anomaly been present? Since birth?. SCP-4409: No, I was born as normal as normal can be. This here was done to me, a long time ago. Its not something I relish contemplating, but if you need to hear it I suppose I dont have a choice.. Dr. Crossley: Im all ears. (SCP-4409 glares at Dr. Crossley for several seconds) Ah, my apologies. Im not supposed to antagonize you unnecessarily. Please, tell me what happened to you?. SCP-4409: Ever hear of Herman Fullers Circus of the Disquieting?. Dr. Crossley: Im afraid I havent.. SCP-4409: There are days I ...
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7-chloro-2-ethyl-4-oxo-3,4-dihydro-6-quinazolinesulfonamide - chemical structural formula, chemical names, chemical properties, synthesis references
Object classification: Safe Special containment procedures: a 5 meter fence has been built around SCP-0001-HV in area 33, no team member is ... by @panconcajeta
Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.. ...
5-tetrahydro-8-chloro-3-methyl-5-phenyl-1h-3-benzazepin-7-ol MeSH D03.438.079.800 - 2,3,4,5-tetrahydro-7,8-dihydroxy-1-phenyl- ... 8-chloro-, 2-acetylhydrazide MeSH D03.494.347.500 - loxapine MeSH D03.494.347.500.040 - amoxapine MeSH D03.494.382.393 - ... 4,5-dihydro-1-(3-(trifluoromethyl)phenyl)-1h-pyrazol-3-amine MeSH D03.383.129.539.200 - epirizole MeSH D03.383.129.539.487 - ... 4-oxadiazole MeSH D03.383.312.649.290 - fanft MeSH D03.383.312.649.308 - furagin MeSH D03.383.312.649.313 - furazolidone MeSH ...
For the extraction of 250 mL of plasma, 4 mL of phosphate buffer (pH 7) was added, and the extraction was carried out at 25°C ... Similarly, FLX was detected in serum using 4-chloro-7-nitro-2,1,3-benzofurazan as a precolumn derivatizing agent in order to ... 51]. Other derivatizing agents have also been used to improve the sensitivity of the assay as 4-fluoro-7-nitrobenzofurazan [52 ... A. Oztunc, A. Onal, and S. Erturk, "7,7,8,8-Tetracyanoquinodimethane as a new derivatization reagent for high-performance ...
摘要: 以4-氯-7-硝基苯并呋咱(NBD-Cl)为原料,设计、合成了3种荧光化合物(NBD-N、NBD-NH、NBD-NH2),通过质谱、核磁共振对其结构进行了表征. 研究表明:这3种化合物在pH 7.0~12.0范围内对水溶液的酸碱度具有可逆的荧光识 ... 7]. LI Y, FAN X W, BAI F Q, et al. Water-soluble fluorescent probe for multiple ions detection based on different pH moderation ... 基于芘希夫碱的汞离子荧光探针的合成与性能研究[J]. 华南师范大学学
SV was labeled with the fluorescent probe 7-octadecylamino-4-nitrobenz-2-oxa-1,3-diazole (NBD-NH-C18) and was allowed to bind ... at 4 degrees C. The effect of lipophosphoglycan (LPG), isolated from Leishmania donovani, on virus fusion was investigated by ... of the value at 4 degrees C, reflecting the disappearance of labeled SV from the cell surface and diffusion of NBD-NH-C18 into ... SV was labeled with the fluorescent probe 7-octadecylamino-4-nitrobenz-2-oxa-1,3-diazole (NBD-NH-C18) and was allowed to bind ...
Arai, G. and M. Onozuka (1979) Mechanism of the reaction of chloro-p-benzoquinones with sodium sulfite. Nippon Kagaku Kaishi, ... A 7, 360-369.. *Tang, Q., Z. Liang, J. Liu, J. Xu and Q. Miao (2010) N-heteroquinones: Quadruple weak hydrogen bonds and n- ... Critical Tables 7, 173-211.. *Brode, W. R. (1943) Chemical Spectroscopy. 2nd Ed., pp. 239-245. John Wiley and Sons, Inc., New ... 4, 50097-50101.. *Heldt, J. R., J. Heldt, M. Stoń and H. A. Diehl (1995) Photophysical properties of 4-alkyl- and 7- ...
Arai, G. and M. Onozuka (1979) Mechanism of the reaction of chloro-p-benzoquinones with sodium sulfite. Nippon Kagaku Kaishi, ... A 7, 360-369.. *Tang, Q., Z. Liang, J. Liu, J. Xu and Q. Miao (2010) N-heteroquinones: Quadruple weak hydrogen bonds and n- ... Critical Tables 7, 173-211.. *Brode, W. R. (1943) Chemical Spectroscopy. 2nd Ed., pp. 239-245. John Wiley and Sons, Inc., New ... 4, 50097-50101.. *Heldt, J. R., J. Heldt, M. Stoń and H. A. Diehl (1995) Photophysical properties of 4-alkyl- and 7- ...
Arai, G. and M. Onozuka (1979) Mechanism of the reaction of chloro-p-benzoquinones with sodium sulfite. Nippon Kagaku Kaishi, ... A 7, 360-369.. *Tang, Q., Z. Liang, J. Liu, J. Xu and Q. Miao (2010) N-heteroquinones: Quadruple weak hydrogen bonds and n- ... Critical Tables 7, 173-211.. *Brode, W. R. (1943) Chemical Spectroscopy. 2nd Ed., pp. 239-245. John Wiley and Sons, Inc., New ... 4, 50097-50101.. *Heldt, J. R., J. Heldt, M. Stoń and H. A. Diehl (1995) Photophysical properties of 4-alkyl- and 7- ...
Arai, G. and M. Onozuka (1979) Mechanism of the reaction of chloro-p-benzoquinones with sodium sulfite. Nippon Kagaku Kaishi, ... A 7, 360-369.. *Tang, Q., Z. Liang, J. Liu, J. Xu and Q. Miao (2010) N-heteroquinones: Quadruple weak hydrogen bonds and n- ... Critical Tables 7, 173-211.. *Brode, W. R. (1943) Chemical Spectroscopy. 2nd Ed., pp. 239-245. John Wiley and Sons, Inc., New ... 4, 50097-50101.. *Heldt, J. R., J. Heldt, M. Stoń and H. A. Diehl (1995) Photophysical properties of 4-alkyl- and 7- ...
Seed germination occurred over a broad range of temperatures (17/7, 25/10, and 30/20 C), but germination being highest at ... 4-chloro-7-nitrobenzofurazan, did not indicate involvement of any metabolic enzymes inhibited by these compounds. The absence ... but there was no difference in the final germination percentage between 4 and 22.8 C. The base temperature was determined to be ...
Isolation of acetylated ESAT-6 was achieved via ASB-14 or 6M guanidine and was confirmed via 4-chloro-7-nitrobenzofurazan (NBD- ... Isolation of acetylated ESAT-6 was achieved via ASB-14 or 6M guanidine and was confirmed via 4-chloro-7-nitrobenzofurazan (NBD- ...
Arai, G. and M. Onozuka (1979) Mechanism of the reaction of chloro-p-benzoquinones with sodium sulfite. Nippon Kagaku Kaishi, ... A 7, 360-369.. *Tang, Q., Z. Liang, J. Liu, J. Xu and Q. Miao (2010) N-heteroquinones: Quadruple weak hydrogen bonds and n- ... Critical Tables 7, 173-211.. *Brode, W. R. (1943) Chemical Spectroscopy. 2nd Ed., pp. 239-245. John Wiley and Sons, Inc., New ... 4, 50097-50101.. *Heldt, J. R., J. Heldt, M. Stoń and H. A. Diehl (1995) Photophysical properties of 4-alkyl- and 7- ...
... This is the entry page of medical terms started with 7. Please click to navigate more by the ... 7 Chloro N methyl 5 phenyl 3H 1,4 benzodiazepin 2 amine 4 oxide ... 7-Chloro-N-methyl-5-phenyl-3H-1,4-benzodiazepin-2-amine 4-oxide ... 7-Chloro-1,3-dihydro-1-methyl-5-phenyl-2H-1,4-benzodiazepin-2-one ... Further Alphabetical Sectioning for Medical Terms: 7. *7*. *7a ...
4-Benzoyl-L-phenylalanine. Bzl. Benzyl. BC-NH2 [O6-(4-Aminomethyl-benzyl)guanine]. Resorufin benzyl ether *CAS 87687-02-3*. BG- ... 4-Methoxybenzenesulfonyl. Mbzl. 4-Methylbenzyl. BC-NH2 [O6-(4-Aminomethyl-benzyl)guanine]. BG-NH2 [O6-(4-Aminomethyl-benzyl) ... 7-Aminoheptanoic acid. Ahx. 6-Aminohexanoic acid (Ahx, C6). C6. DNP-X acid [6-(2,4-Dinitrophenyl)aminohexanoic acid] *CAS 10466 ... 4-MbNA. 4-Methoxy-β-naphthylamide. AFC. 7-Amino-4-trifluoromethyl-coumarin. AFC [7-Amino-4-trifluoromethylcoumarin] *CAS 53518- ...
... This is the entry page of medical terms started with 4. Please click to navigate more by the ...
Arai, G. and M. Onozuka (1979) Mechanism of the reaction of chloro-p-benzoquinones with sodium sulfite. Nippon Kagaku Kaishi, ... A 7, 360-369.. *Tang, Q., Z. Liang, J. Liu, J. Xu and Q. Miao (2010) N-heteroquinones: Quadruple weak hydrogen bonds and n- ... Critical Tables 7, 173-211.. *Brode, W. R. (1943) Chemical Spectroscopy. 2nd Ed., pp. 239-245. John Wiley and Sons, Inc., New ... 4, 50097-50101.. *Heldt, J. R., J. Heldt, M. Stoń and H. A. Diehl (1995) Photophysical properties of 4-alkyl- and 7- ...
Arai, G. and M. Onozuka (1979) Mechanism of the reaction of chloro-p-benzoquinones with sodium sulfite. Nippon Kagaku Kaishi, ... A 7, 360-369.. *Tang, Q., Z. Liang, J. Liu, J. Xu and Q. Miao (2010) N-heteroquinones: Quadruple weak hydrogen bonds and n- ... Critical Tables 7, 173-211.. *Brode, W. R. (1943) Chemical Spectroscopy. 2nd Ed., pp. 239-245. John Wiley and Sons, Inc., New ... 4, 50097-50101.. *Heldt, J. R., J. Heldt, M. Stoń and H. A. Diehl (1995) Photophysical properties of 4-alkyl- and 7- ...
Arai, G. and M. Onozuka (1979) Mechanism of the reaction of chloro-p-benzoquinones with sodium sulfite. Nippon Kagaku Kaishi, ... A 7, 360-369.. *Tang, Q., Z. Liang, J. Liu, J. Xu and Q. Miao (2010) N-heteroquinones: Quadruple weak hydrogen bonds and n- ... Critical Tables 7, 173-211.. *Brode, W. R. (1943) Chemical Spectroscopy. 2nd Ed., pp. 239-245. John Wiley and Sons, Inc., New ... 4, 50097-50101.. *Heldt, J. R., J. Heldt, M. Stoń and H. A. Diehl (1995) Photophysical properties of 4-alkyl- and 7- ...
Arai, G. and M. Onozuka (1979) Mechanism of the reaction of chloro-p-benzoquinones with sodium sulfite. Nippon Kagaku Kaishi, ... A 7, 360-369.. *Tang, Q., Z. Liang, J. Liu, J. Xu and Q. Miao (2010) N-heteroquinones: Quadruple weak hydrogen bonds and n- ... Critical Tables 7, 173-211.. *Brode, W. R. (1943) Chemical Spectroscopy. 2nd Ed., pp. 239-245. John Wiley and Sons, Inc., New ... 4, 50097-50101.. *Heldt, J. R., J. Heldt, M. Stoń and H. A. Diehl (1995) Photophysical properties of 4-alkyl- and 7- ...
Arai, G. and M. Onozuka (1979) Mechanism of the reaction of chloro-p-benzoquinones with sodium sulfite. Nippon Kagaku Kaishi, ... A 7, 360-369.. *Tang, Q., Z. Liang, J. Liu, J. Xu and Q. Miao (2010) N-heteroquinones: Quadruple weak hydrogen bonds and n- ... Critical Tables 7, 173-211.. *Brode, W. R. (1943) Chemical Spectroscopy. 2nd Ed., pp. 239-245. John Wiley and Sons, Inc., New ... 4, 50097-50101.. *Heldt, J. R., J. Heldt, M. Stoń and H. A. Diehl (1995) Photophysical properties of 4-alkyl- and 7- ...
Arai, G. and M. Onozuka (1979) Mechanism of the reaction of chloro-p-benzoquinones with sodium sulfite. Nippon Kagaku Kaishi, ... A 7, 360-369.. *Tang, Q., Z. Liang, J. Liu, J. Xu and Q. Miao (2010) N-heteroquinones: Quadruple weak hydrogen bonds and n- ... Critical Tables 7, 173-211.. *Brode, W. R. (1943) Chemical Spectroscopy. 2nd Ed., pp. 239-245. John Wiley and Sons, Inc., New ... 4, 50097-50101.. *Heldt, J. R., J. Heldt, M. Stoń and H. A. Diehl (1995) Photophysical properties of 4-alkyl- and 7- ...
Arai, G. and M. Onozuka (1979) Mechanism of the reaction of chloro-p-benzoquinones with sodium sulfite. Nippon Kagaku Kaishi, ... A 7, 360-369.. *Tang, Q., Z. Liang, J. Liu, J. Xu and Q. Miao (2010) N-heteroquinones: Quadruple weak hydrogen bonds and n- ... Critical Tables 7, 173-211.. *Brode, W. R. (1943) Chemical Spectroscopy. 2nd Ed., pp. 239-245. John Wiley and Sons, Inc., New ... 4, 50097-50101.. *Heldt, J. R., J. Heldt, M. Stoń and H. A. Diehl (1995) Photophysical properties of 4-alkyl- and 7- ...
Arai, G. and M. Onozuka (1979) Mechanism of the reaction of chloro-p-benzoquinones with sodium sulfite. Nippon Kagaku Kaishi, ... A 7, 360-369.. *Tang, Q., Z. Liang, J. Liu, J. Xu and Q. Miao (2010) N-heteroquinones: Quadruple weak hydrogen bonds and n- ... Critical Tables 7, 173-211.. *Brode, W. R. (1943) Chemical Spectroscopy. 2nd Ed., pp. 239-245. John Wiley and Sons, Inc., New ... 4, 50097-50101.. *Heldt, J. R., J. Heldt, M. Stoń and H. A. Diehl (1995) Photophysical properties of 4-alkyl- and 7- ...
5-Amino-3-((5-nitro-2-furyl)vinyl)-1,2,4-oxadiazole [D03.383.129.462.580.200] ... This graph shows the total number of publications written about "5-Amino-3-((5-nitro-2-furyl)vinyl)-1,2,4-oxadiazole" by people ... Below are the most recent publications written about "5-Amino-3-((5-nitro-2-furyl)vinyl)-1,2,4-oxadiazole" by people in ... "5-Amino-3-((5-nitro-2-furyl)vinyl)-1,2,4-oxadiazole" is a descriptor in the National Library of Medicine's controlled ...
Synthesis of substituted tetrahydrofurans via intermolecular reactions of γ-chlorocarbanions of 3-substituted 3-chloro- ... سنتز 4- (4-تولون سولفونیل) کینولین از نیتروآرن ها و آللی سولفون ها با استفاده از روش گام به گام ... Synthesis of 4-(4-toluenesulfonyl)quinolines from nitroarenes and allyl sulfones using step-by-step procedure ... دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2011 سفارش ترجمه ...
4 [(1 tert butyl 1h tetrazol 5 yl)(piperidin 1 yl)methyl] 6 methoxyquinoline; antimalarial agent;.... None. View. ... 5,5' dihydroxy 4',7 dimethoxyflavanone 5,5' di o beta dextro glucopyranoside; 5,7 dihydroxy 4' me.... None. View. ... 6,7 dihydro 2 nitro 6 (4 trifluoromethoxybenzyloxy) 5h imidazo[2,1 b][1,3]oxazine; bedaquiline; i.... None. View. ... 4 [(2 hydroxy 7 (3 oxobutyl)naphthalen 1 yl)diazenyl] 3,5 dinitrobenzoic acid; 4 [(2 hydroxynapht.... None. View. ...
SV was labeled with the fluorescent probe 7-octadecylamino-4-nitrobenz-2-oxa-1,3-diazole (NBD-NH-C18) and was allowed to bind ... at 4 degrees C. The effect of lipophosphoglycan (LPG), isolated from Leishmania donovani, on virus fusion was investigated by ... of the value at 4 degrees C, reflecting the disappearance of labeled SV from the cell surface and diffusion of NBD-NH-C18 into ... SV was labeled with the fluorescent probe 7-octadecylamino-4-nitrobenz-2-oxa-1,3-diazole (NBD-NH-C18) and was allowed to bind ...
5-tetrahydro-8-chloro-3-methyl-5-phenyl-1h-3-benzazepin-7-ol MeSH D03.438.079.800 - 2,3,4,5-tetrahydro-7,8-dihydroxy-1-phenyl- ... 8-chloro-, 2-acetylhydrazide MeSH D03.494.347.500 - loxapine MeSH D03.494.347.500.040 - amoxapine MeSH D03.494.382.393 - ... 4,5-dihydro-1-(3-(trifluoromethyl)phenyl)-1h-pyrazol-3-amine MeSH D03.383.129.539.200 - epirizole MeSH D03.383.129.539.487 - ... 4-oxadiazole MeSH D03.383.312.649.290 - fanft MeSH D03.383.312.649.308 - furagin MeSH D03.383.312.649.313 - furazolidone MeSH ...
For the extraction of 250 mL of plasma, 4 mL of phosphate buffer (pH 7) was added, and the extraction was carried out at 25°C ... Similarly, FLX was detected in serum using 4-chloro-7-nitro-2,1,3-benzofurazan as a precolumn derivatizing agent in order to ... 51]. Other derivatizing agents have also been used to improve the sensitivity of the assay as 4-fluoro-7-nitrobenzofurazan [52 ... A. Oztunc, A. Onal, and S. Erturk, "7,7,8,8-Tetracyanoquinodimethane as a new derivatization reagent for high-performance ...
4 [(1 tert butyl 1h tetrazol 5 yl)(piperidin 1 yl)methyl] 6 methoxyquinoline; antimalarial agent;.... None. View. ... 5,5 dihydroxy 4,7 dimethoxyflavanone 5,5 di o beta dextro glucopyranoside; 5,7 dihydroxy 4 me.... None. View. ... 6,7 dihydro 2 nitro 6 (4 trifluoromethoxybenzyloxy) 5h imidazo[2,1 b][1,3]oxazine; bedaquiline; i.... None. View. ... 4 [(2 hydroxy 7 (3 oxobutyl)naphthalen 1 yl)diazenyl] 3,5 dinitrobenzoic acid; 4 [(2 hydroxynapht.... None. View. ...
Isolation of acetylated ESAT-6 was achieved via ASB-14 or 6M guanidine and was confirmed via 4-chloro-7-nitrobenzofurazan (NBD- ... Isolation of acetylated ESAT-6 was achieved via ASB-14 or 6M guanidine and was confirmed via 4-chloro-7-nitrobenzofurazan (NBD- ...
摘要: 以4-氯-7-硝基苯并呋咱(NBD-Cl)为原料,设计、合成了3种荧光化合物(NBD-N、NBD-NH、NBD-NH2),通过质谱、核磁共振对其结构进行了表征. 研究表明:这3种化合物在pH 7.0~12.0范围内对水溶液的酸碱度具有可逆的荧光识 ... 7]. LI Y, FAN X W, BAI F Q, et al. Water-soluble fluorescent probe for multiple ions detection based on different pH moderation ... 基于芘希夫碱的汞离子荧光探针的合成与性能研究[J]. 华南师范大学学
Seed germination occurred over a broad range of temperatures (17/7, 25/10, and 30/20 C), but germination being highest at ... 4-chloro-7-nitrobenzofurazan, did not indicate involvement of any metabolic enzymes inhibited by these compounds. The absence ... but there was no difference in the final germination percentage between 4 and 22.8 C. The base temperature was determined to be ...
Synthesis of substituted tetrahydrofurans via intermolecular reactions of γ-chlorocarbanions of 3-substituted 3-chloro- ... سنتز 4- (4-تولون سولفونیل) کینولین از نیتروآرن ها و آللی سولفون ها با استفاده از روش گام به گام ... Synthesis of 4-(4-toluenesulfonyl)quinolines from nitroarenes and allyl sulfones using step-by-step procedure ... دانلود رایگان متن کامل مقاله ISI 4 صفحه سال انتشار : 2011 سفارش ترجمه ...
... This is the entry page of medical terms started with 7. Please click to navigate more by the ... 7 Chloro N methyl 5 phenyl 3H 1,4 benzodiazepin 2 amine 4 oxide ... 7-Chloro-N-methyl-5-phenyl-3H-1,4-benzodiazepin-2-amine 4-oxide ... 7-Chloro-1,3-dihydro-1-methyl-5-phenyl-2H-1,4-benzodiazepin-2-one ... Further Alphabetical Sectioning for Medical Terms: 7. *7*. *7a ...
5-Amino-3-((5-nitro-2-furyl)vinyl)-1,2,4-oxadiazole [D03.383.129.462.580.200] ... This graph shows the total number of publications written about "5-Amino-3-((5-nitro-2-furyl)vinyl)-1,2,4-oxadiazole" by people ... Below are the most recent publications written about "5-Amino-3-((5-nitro-2-furyl)vinyl)-1,2,4-oxadiazole" by people in ... "5-Amino-3-((5-nitro-2-furyl)vinyl)-1,2,4-oxadiazole" is a descriptor in the National Library of Medicines controlled ...
Arai, G. and M. Onozuka (1979) Mechanism of the reaction of chloro-p-benzoquinones with sodium sulfite. Nippon Kagaku Kaishi, ... A 7, 360-369.. *Tang, Q., Z. Liang, J. Liu, J. Xu and Q. Miao (2010) N-heteroquinones: Quadruple weak hydrogen bonds and n- ... Critical Tables 7, 173-211.. *Brode, W. R. (1943) Chemical Spectroscopy. 2nd Ed., pp. 239-245. John Wiley and Sons, Inc., New ... 4, 50097-50101.. *Heldt, J. R., J. Heldt, M. Stoń and H. A. Diehl (1995) Photophysical properties of 4-alkyl- and 7- ...
Arai, G. and M. Onozuka (1979) Mechanism of the reaction of chloro-p-benzoquinones with sodium sulfite. Nippon Kagaku Kaishi, ... A 7, 360-369.. *Tang, Q., Z. Liang, J. Liu, J. Xu and Q. Miao (2010) N-heteroquinones: Quadruple weak hydrogen bonds and n- ... Critical Tables 7, 173-211.. *Brode, W. R. (1943) Chemical Spectroscopy. 2nd Ed., pp. 239-245. John Wiley and Sons, Inc., New ... 4, 50097-50101.. *Heldt, J. R., J. Heldt, M. Stoń and H. A. Diehl (1995) Photophysical properties of 4-alkyl- and 7- ...
To characterize the vesicular interactions of the C. pneumoniae inclusion, we used a fluorescent analogue of ceramide, (N-[7-(4 ...
MeSH Terms: 4-Chloro-7-nitrobenzofurazan/analogs & derivatives; 4-Chloro-7-nitrobenzofurazan/chemistry; 4-Chloro-7- ... nitrobenzofurazan/metabolism; Allosteric Regulation; Apoproteins; Binding Sites; Databases, Protein; Fluorescent Dyes/chemistry ...
4. The ultrastructure of E. chaffeensis was impaired by MβCD treatment. Host cell-free E. chaffeensis was treated with 10 mM M ... 2010 Feb 10;167(2-4):155-66. doi: 10.1016/j.vetpar.2009.09.017. Epub 2009 Sep 19. Vet Parasitol. 2010. PMID: 19836896 Free PMC ... 2022 Aug 30;13(4):e0070322. doi: 10.1128/mbio.00703-22. Epub 2022 Jul 18. mBio. 2022. PMID: 35862781 Free PMC article. ...
2008 Jan 4;375(1):240-56. doi: 10.1016/j.jmb.2007.10.038. Epub 2007 Oct 22. J Mol Biol. 2008. PMID: 18005990 ... Figure 7. Model for lipid-induction of apoC-II fibril assembly The model proposes a lipid-induced tetramerisation followed by a ... Figure 4 Sedimentation velocity analysis of the interaction between NBD-Lyso-12-PC and apoC-II. Sedimentation velocity data ... 2015 Feb 18;35(7):2871-84. doi: 10.1523/JNEUROSCI.2912-14.2015. J Neurosci. 2015. PMID: 25698727 Free PMC article. ...
200 5-Amino-3-((5-nitro-2-furyl)vinyl)-1,2,4-oxadiazol e D02.640.600.290 FANFT D02. ... 200 5-Amino-3-((5-nitro-2-furyl)vinyl)- ... 2 , Vinyl Cyanide (See Acrylonitrile) Vinyl Cyclohexene 14,2 2581 - wee STL Marked Irritation s s 4 Dioxide Skin/Suspect . ... NLM Digital Collections - Index-catalogue of the Library of the Surgeon Generals Office, United States Army (Series 4, ... ... J. M. lechn., 1944, 6: 1-4.-Burg, A. B. The behavior of trimethyl- amine, tt.methylamminosulfur trioxide and trimethylamine ...
Biomacromolecules, 12(7), 2524-2533 (2011-05-25). Nanopharmaceutics composed of a carrier and a protein have the potential to ... Scientific reports, 7(1), 10945-10945 (2017-09-10). We report a simple single-molecule fluorescence imaging method that ... The monodispersity of alpha allowed the studies of nucleotide-binding and inhibitory effect of 4-Chloro-7-nitrobenzofurazan ( ...
4-Chloro-7-nitrobenzofurazan Preferred Term Term UI T027826. Date08/07/1981. LexicalTag NON. ThesaurusID ... 4-Chloro-7-nitrobenzofurazan Preferred Concept UI. M0014530. Registry Number. EQF2794IRE. Related Numbers. 10199-89-0. Scope ... 7-Chloro-4-nitrobenzofurazan Term UI T027827. Date08/07/1981. LexicalTag NON. ThesaurusID ... 7-Chloro-4-nitrobenzofurazan Chloronitrobenzoxadiazole NBD Chloride NBF-Cl Nitrobenzoxadiazole Chloride Pharm Action. Enzyme ...
4-Chloro-7-nitrobenzofurazan Preferred Term Term UI T027826. Date08/07/1981. LexicalTag NON. ThesaurusID ... 4-Chloro-7-nitrobenzofurazan Preferred Concept UI. M0014530. Registry Number. EQF2794IRE. Related Numbers. 10199-89-0. Scope ... 7-Chloro-4-nitrobenzofurazan Term UI T027827. Date08/07/1981. LexicalTag NON. ThesaurusID ... 7-Chloro-4-nitrobenzofurazan Chloronitrobenzoxadiazole NBD Chloride NBF-Cl Nitrobenzoxadiazole Chloride Pharm Action. Enzyme ...
1,3-butadiene, 2-chloro-, Homopolymer. *Polychloroprene. *Chloroprene Polymer. o-Chlorobenzylidenemalonitrile *O- ... 514 results in 0.056 seconds 26 pages viewing page 7 of 4-Chloro-7-nitrobenzofurazan *4-chloro-7-nitrobenzofurazan ...
5-Tetrahydro-8-chloro-3-methyl-5-phenyl-1H-3-benzazepin-7-ol N0000166927 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3- ... 8-chloro-, 2-acetylhydrazide N0000178916 Edetic Acid, Disodium Salt N0000169572 Fatty Acid-Binding Proteins N0000005700 ... 4-Diisocyanate N0000167971 L-Iditol 2-Dehydrogenase N0000166931 2H-Benzo(a)quinolizin-2-ol, 2-Ethyl-1,3,4,6,7,11b-hexahydro-3- ... 4,4-(3-oxo-1,5-pentanediyl)bis(N,N-dimethyl-N-2-propenyl-), Dibromide N0000168635 Phosphatidylinositol 4,5-Diphosphate ...
2008;4:1105-15 pubmed publisher *. Langemeyer L, Engelbrecht S. Essential arginine in subunit a and aspartate in subunit c of ... 2009;191:2400-4 pubmed publisher ..Independent of the carbon source used for growth and independent of the presence of other ... 2008;283:9871-7 pubmed publisher ..Positive charges in the cytoplasmic loop of F(0)c are important determinants for YidC ... 2006;281:37861-7 pubmed ..Other key residues in TMHs 2, 4, and 5, which were concluded previously to compose a possible aqueous ...
Immunoblots showed cross reactivity between a 53 kDa peptide and anti-$ (chloro- plast F(), as well as between 53, 50 and 47 ... Furthermore, the ATPase was inactivated by preincubation with 7-chloro-4-nitro-benzofurazan (NBD-C1). The ATPase activity of H ...
2019; 10(1): 20-25 » doi: 10.5530/srp.2019.1.4. *A Review of Pharmacoeconomics: the key to "Healthcare for All" Hasamnis AA, ... by reaction with 4-chloro-7-nitrobenzofurazan (NBD-Cl) as reagent in an alkaline interemediate. These methods are summarized on ...
InChI=1/C9H15O6P/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15/h1-6H2,(H,10,11)(H,12,13)(H,14,15)/p-3. ...
4-DINITROPHENOL DYES ACRIDINE ORANGE DYES ACRIFLAVINE DYES ALCIAN BLUE DYES AMARANTH DYE DYES AMIDO BLACK DYES AMINACRINE DYES ... 5-DIMETHOXY-4-METHYLAMPHETAMINE CENTRAL NERVOUS SYSTEM AGENTS 3,4-DICHLORO-N-METHYL-N-(2-(1-PYRROLIDI CENTRAL NERVOUS SYSTEM ... AND PE 7,8-DIHYDRO-7,8-DIHYDROXYBENZO(A)PYRENE ENVIRONMENTAL POLLUTANTS, NOXAE, AND PE 9,10-DIMETHYL-1,2-BENZANTHRACENE ... 4-DICHLORO-N-METHYL-N-(2-(1-PYRROLIDI SENSORY SYSTEM AGENTS ACETAMINOPHEN SENSORY SYSTEM AGENTS ADENOSINE SENSORY SYSTEM AGENTS ...
4-Chloro-7-nitrobenzofurazan, Animals, Anti-Bacterial Agents, Biological Transport, Active, Brefeldin A, Cell Membrane, ...
  • This is the entry page of medical terms started with 7. (charmhtml.com)
  • Furthermore, the ATPase was inactivated by preincubation with 7-chloro-4-nitro-benzofurazan (NBD-C1). (mpg.de)
  • Immunoblots showed cross reactivity between a 53 kDa peptide and anti-$ (chloro- plast F(), as well as between 53, 50 and 47 kDa peptides and an ATPase antibody of Methano-sarcina barkeri. (mpg.de)
  • The monodispersity of alpha allowed the studies of nucleotide-binding and inhibitory effect of 4-Chloro-7-nitrobenzofurazan (NBD-Cl) to ATP/ADP-binding. (sigmaaldrich.com)
  • Langemeyer L, Engelbrecht S. Essential arginine in subunit a and aspartate in subunit c of FoF1 ATP synthase: effect of repositioning within helix 4 of subunit a and helix 2 of subunit c. (labome.org)