A benzofuran derivative used as a protein reagent since the terminal N-NBD-protein conjugate possesses interesting fluorescence and spectral properties. It has also been used as a covalent inhibitor of both beef heart mitochondrial ATPase and bacterial ATPase.
Very toxic and complex pyrone derivatives from the fungus Calcarisporium arbuscula. They bind to and inhibit mitochondrial ATPase, thereby uncoupling oxidative phosphorylation. They are used as biochemical tools.
A sulfhydryl proteinase with cysteine at the active site from ficus latex. Preferential cleavage is at tyrosine and phenylalanine residues. EC 3.4.22.3.
Compounds that contain a BENZENE ring fused to a furan ring.
Multisubunit enzymes that reversibly synthesize ADENOSINE TRIPHOSPHATE. They are coupled to the transport of protons across a membrane.
A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.
The mitochondria of the myocardium.
2-Chloroadenosine. A metabolically stable analog of adenosine which acts as an adenosine receptor agonist. The compound has a potent effect on the peripheral and central nervous system.
A family of nonmetallic, generally electronegative, elements that form group 17 (formerly group VIIa) of the periodic table.
Compounds containing the -SH radical.
A centrally active muscarinic antagonist that has been used in the symptomatic treatment of PARKINSON DISEASE. Benztropine also inhibits the uptake of dopamine.
The rate dynamics in chemical or physical systems.
An essential amino acid. It is often added to animal feed.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
A greenish-yellow, diatomic gas that is a member of the halogen family of elements. It has the atomic symbol Cl, atomic number 17, and atomic weight 70.906. It is a powerful irritant that can cause fatal pulmonary edema. Chlorine is used in manufacturing, as a reagent in synthetic chemistry, for water purification, and in the production of chlorinated lime, which is used in fabric bleaching.
A group of 1,2-benzenediols that contain the general formula R-C6H5O2.
Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.
The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Formation of an acetyl derivative. (Stedman, 25th ed)
Aminopeptidases that remove METHIONINE from the amino-terminus of a peptide chain, such as the initiator METHIONINE found on nascent peptide chains.
An N-terminal acetyltransferase subtype that consists of the Naa10p catalytic subunit and the Naa15p auxiliary subunit. The structure of this enzyme is conserved between lower and higher eukaryotes. It has specificity for N-terminal SERINE; ALANINE; THREONINE; GLYCINE; VALINE; and CYSTINE residues and acts on nascent peptide chains after the removal of the initiator METHIONINE by METHIONYL AMINOPEPTIDASES.
An N-terminal acetyltransferase subtype that consists of the Naa20p catalytic subunit and the Naa25p auxiliary subunit. The structure of this enzyme is conserved between YEASTS and HUMAN. It has specificity for the N-terminal METHIONINE of peptides where the next amino acid in the chain is either ASPARTATE; GLUTAMATE; ASPARAGINE; OR GLUTAMINE.
An N-terminal acetyltransferase subtype that consists of the Naa50p catalytic subunit, and the Naa10p and Naa15p auxiliary subunits. It has specificity for the N-terminal METHIONINE of peptides where the next amino acid in the chain is hydrophobic.
A species of gram-positive, asporogenous, non-pathogenic, soil bacteria that produces GLUTAMIC ACID.
A genus of asporogenous bacteria that is widely distributed in nature. Its organisms appear as straight to slightly curved rods and are known to be human and animal parasites and pathogens.
An enzyme catalyzing the oxidation of 2 moles of glutathione in the presence of hydrogen peroxide to yield oxidized glutathione and water. EC 1.11.1.9.
The genetic unit consisting of three structural genes, an operator and a regulatory gene. The regulatory gene controls the synthesis of the three structural genes: BETA-GALACTOSIDASE and beta-galactoside permease (involved with the metabolism of lactose), and beta-thiogalactoside acetyltransferase.
A species of ORTHOPOXVIRUS causing an epidemic disease among captive primates.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
A viral disease infecting PRIMATES and RODENTS. Its clinical presentation in humans is similar to SMALLPOX including FEVER; HEADACHE; COUGH; and a painful RASH. It is caused by MONKEYPOX VIRUS and is usually transmitted to humans through BITES or via contact with an animal's BLOOD. Interhuman transmission is relatively low (significantly less than smallpox).
A competitive histamine H2-receptor antagonist. Its main pharmacodynamic effect is the inhibition of gastric secretion.
A non-imidazole blocker of those histamine receptors that mediate gastric secretion (H2 receptors). It is used to treat gastrointestinal ulcers.
A publication issued at stated, more or less regular, intervals.
A histamine congener, it competitively inhibits HISTAMINE binding to HISTAMINE H2 RECEPTORS. Cimetidine has a range of pharmacological actions. It inhibits GASTRIC ACID secretion, as well as PEPSIN and GASTRIN output.
Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Individual's rights to obtain and use information collected or generated by others.
A benzothiazepine derivative with vasodilating action due to its antagonism of the actions of CALCIUM ion on membrane functions.
Drugs that block the transport of adrenergic transmitters into axon terminals or into storage vesicles within terminals. The tricyclic antidepressants (ANTIDEPRESSIVE AGENTS, TRICYCLIC) and amphetamines are among the therapeutically important drugs that may act via inhibition of adrenergic transport. Many of these drugs also block transport of serotonin.
Compounds that specifically inhibit the reuptake of serotonin in the brain.
The first highly specific serotonin uptake inhibitor. It is used as an antidepressant and often has a more acceptable side-effects profile than traditional antidepressants.
Mood-stimulating drugs used primarily in the treatment of affective disorders and related conditions. Several MONOAMINE OXIDASE INHIBITORS are useful as antidepressants apparently as a long-term consequence of their modulation of catecholamine levels. The tricyclic compounds useful as antidepressive agents (ANTIDEPRESSIVE AGENTS, TRICYCLIC) also appear to act through brain catecholamine systems. A third group (ANTIDEPRESSIVE AGENTS, SECOND-GENERATION) is a diverse group of drugs including some that act specifically on serotonergic systems.
A furancarbonitrile that is one of the SEROTONIN UPTAKE INHIBITORS used as an antidepressant. The drug is also effective in reducing ethanol uptake in alcoholics and is used in depressed patients who also suffer from tardive dyskinesia in preference to tricyclic antidepressants, which aggravate this condition.
A serotonin uptake inhibitor that is effective in the treatment of depression.

Specific and sensitive assay for alkaline and neutral ceramidases involving C12-NBD-ceramide. (1/347)

A fluorescent analogue of ceramide, C12-NBD-ceramide, was found to be hydrolyzed much faster than 14C-labeled ceramide by alkaline ceramidase from Pseudomonas aeruginosa and neutral ceramidase from mouse liver, while this substrate was relatively resistant to acid ceramidase from plasma of the horseshoe crab. The radioactive substrate was used more preferentially by the acid ceramidase. It should be noted that C6-NBD-ceramide, which is usually used for ceramidase assays, was hardly hydrolyzed by any of the enzymes examined, compared to C12-NBD-ceramide. For the alkaline and neutral enzymes, the Vmax and k (Vmax/Km) with C12-NBD-ceramide were much higher than those with 14C-ceramide. In contrast, for the acid enzyme these parameters with C12-NBD-ceramide were less than half those with the radioisotope-labeled substrate. It is noteworthy that the labeling of ceramide with NBD did not itself reduce the Km of the alkaline enzyme, but did that of the neutral enzyme. It was also found that C12-NBD-ceramide was preferentially hydrolyzed by the alkaline and neutral enzymes, but not the acid one, in several mammalian cell lines. This study clearly shows that the attachment of NBD, but not dansyl, increases the susceptibility of ceramide to alkaline and neutral enzyme, and decreases that to acid enzymes. Thus the use of this substrate provides a specific and sensitive assay for alkaline and neutral ceramidases.  (+info)

Maturation of the axonal plasma membrane requires upregulation of sphingomyelin synthesis and formation of protein-lipid complexes. (2/347)

Neuronal maturation is a gradual process; first axons and dendrites are established as distinct morphological entities; next the different intracellular organization of these processes occurs; and finally the specialized plasma membrane domains of these two compartments are formed. Only when this has been accomplished does proper neuronal function take place. In this work we present evidence that the correct distribution of a class of axonal membrane proteins requires a mechanism which involves formation of protein-lipid (sphingomyelin/cholesterol) detergent-insoluble complexes (DIGs). Using biochemistry and immunofluorescence microscopy we now show that in developing neurons the randomly distributed Thy-1 does not interact with lipids into DIGs (in fully developed neurons the formation of such complexes is essential for the correct axonal targeting of this protein). Using lipid mass spectrometry and thin layer chromatography we show that the DIG lipid missing in the developing neurons is sphingomyelin, but not cholesterol or glucosylceramide. Finally, by increasing the intracellular levels of sphingomyelin in the young neurons the formation of Thy-1/DIGs was induced and, consistent with a role in sorting, proper axonal distribution was facilitated. These results emphasize the role of sphingomyelin in axonal, and therefore, neuronal maturation.  (+info)

Saturable stimulation of fatty acid transport through model cytoplasm by soluble binding protein. (3/347)

To better define the role of soluble binding proteins in the cytoplasmic transport of amphipathic molecules, we measured the diffusional mobility of a fluorescent long-chain fatty acid, 12-N-methyl-(7-nitrobenz-2-oxa-1,3-diazol)aminostearate (NBD-stearate), through model cytoplasm as a function of soluble binding protein concentration. Diffusional mobilities were correlated with the partition of the fatty acid between membrane and protein binding sites. Cytoplasm was modeled as a dense suspension of liposomes, and albumin was used as a model binding protein. Albumin saturably increased NBD-stearate mobility through the membrane suspension approximately eightfold. Fatty acid mobility in the absence of albumin was identical to the mobility of the membrane vesicles (1.99 +/- 0.33 x 10(-8) cm(2)/s), whereas the mobility at saturating concentrations was identical to the mobility of albumin (1.65 +/- 0.12 x 10(-7) cm(2)/s). The protein concentration producing half-maximal stimulation of NBD-stearate diffusion (42.8 +/- 0.3 microM) was unexpectedly greater than that required to solubilize half of the NBD-stearate (17.9 +/- 3.0 microM). These results support a proposed mechanism for cytoplasmic transport of small amphipathic molecules in which aqueous diffusion of the protein-bound form of the molecule largely determines the transport rate. However, slow interchange of fatty acid between the binding protein and membranes also appears to influence the transport rate in this model system.  (+info)

Fluorescent phosphoinositide derivatives reveal specific binding of gelsolin and other actin regulatory proteins to mixed lipid bilayers. (4/347)

Fluorescent derivatives of phosphatidyl inositol (PtdIns)-(4,5)-P2 were synthesized and used to test the effects of the PtdIns-(4, 5)-P2-regulated proteins gelsolin, tau, cofilin, and profilin on labeled PtdIns-(4,5)-P2 that was either in micellar form or mixed with phosphatidylcholine (PtdCho) in bilayer vesicles. Gelsolin increased the fluorescence of 7-nitrobenz-2-oxa-1,3-diazole (NBD)- or pyrene-labeled PtdIns-(4,5)-P2 and NBD-PtdIns-(3,4,5)-P3. Cofilin and profilin produced no detectable change at equimolar ratios to PtdIns-(4,5)-P2, while tau decreased NBD-PtdIns-(4,5)-P2 fluorescence. Fluorescence enhancement by gelsolin of NBD-PtdIns-(4, 5)-P2 in mixed lipid vesicles depended on the mole fraction of PtdIns-(4,5)-P2 in the bilayer. Specific enhancement of 3% NBD-PtdIns-(4,5)-P2 : 97% PtdCho was much lower than that of 10% PtdIns-(4,5)-P2 : 90% PtdCho, but the enhancement of 3% NBD-PtdIns-(4,5)-P2 could be increased by addition of 7% unlabeled PtdIns-(4,5)-P2. The gelsolin-dependent increase in NBD-PtdIns-(4, 5)-P2 fluorescence was reversed by addition of Ca2+ or G-actin. Significant, but weaker, fluorescence enhancement was observed with the gelsolin N-terminal domain (residues 1-160) and a peptide comprised of gelsolin residues 150-169. Fluorescence energy transfer from gelsolin to pyrene-PtdIns-(4,5)-P2 was much stronger with intact gelsolin than the N-terminal region of gelsolin containing the PtdIns-(4,5)-P2 binding sites, suggesting that PtdIns-(4,5)-P2 may bind near a site formed by the juxtaposition of the N- and C-terminal domains of gelsolin.  (+info)

Rapid transbilayer movement of fluorescent phospholipid analogues in the plasma membrane of endocytosis-deficient yeast cells does not require the Drs2 protein. (5/347)

Evidence is presented that endocytosis-deficient Saccharomyces cerevisiae end4 yeast cells rapidly internalize the fluorescent phospholipid analogues 1-palmitoyl-2-{6-[7-nitro-2,1, 3-benzoxadiazol-4-yl(NBD)amino] caproyl}phosphatidylcholine (P-C6-NBD-PtdCho) and P-C6-NBD-phosphatidylserine (P-C6-NBD-PtdSer). Both analogues redistributed between the exoplasmic and cytoplasmic leaflet with a half-time of < 15 min at 0 degrees C. The plateau of internalized analogues was about 70%. Transbilayer movement is probably protein-mediated, as the flip-flop of both analogues was very slow in liposomes composed of plasma-membrane lipids. Rapid analogue internalization was not abolished on depletion of intracellular ATP by about 90%. For P-C6-NBD-PtdCho only was a moderate decrease in the plateau of internalized analogues of about 20% observed, while that of P-C6-NBD-PtdSer was not affected. The Drs2 protein plays only a minor role, if any, in the rapid transbilayer movement of analogues in S. cerevisiae end4 cells. In S. cerevisiae end4 Deltadrs2 cells harbouring both an end4 allele and a drs2 null allele, about 60% and 50% of P-C6-NBD-PtdCho and P-C6-NBD-PtdSer, respectively, became internalized within 15 min at 0 degrees C. The preferential orientation of P-C6-NBD-PtdSer to the cytoplasmic leaflet is in qualitative agreement with the sequestering of endogenous phosphatidylserine to the cytoplasmic leaflet, as assessed by binding of annexin V. Virtually no binding of annexin V to spheroplasts of the parent wild-type strain or the mutant strains was observed. Likewise, no difference in the exposure of endogenous aminophospholipids to the exoplasmic leaflet between these strains was found by labelling with trinitrobenzenesulfonic acid. Thus, lipid asymmetry, at least of aminophospholipids, was preserved in S. cerevisiae end4 cells independently of the presence of the Drs2 protein.  (+info)

Cytoplasmic transport of fatty acids in rat enterocytes: role of binding to fatty acid-binding protein. (6/347)

The intracellular movement of fatty acids is thought to be facilitated through codiffusion with fatty acid-binding protein (FABP). This facilitation may occur by decreasing binding to immobile membranes, leading to faster cytoplasmic diffusion. The aims of this study were to measure the intracellular transport of 12-N-methyl-(7-nitrobenzo-2-oxa-1,3-diazol)aminostearate (NBD-stearate) in villus rat enterocytes and to determine 1) the mechanism of its cytoplasmic transport and 2) if its transport rate correlated with the known variation of FABP binding capacity along the length of the small intestine. Two-dimensional laser photobleaching was used to measure the movement of a fluorescent fatty acid NBD-stearate in enterocytes isolated from different segments of rat intestine. The fraction of NBD-stearate found in the cytostol of enterocytes was determined by differential centrifugation. Cytoplasmic transport of NBD-stearate occurred solely by diffusion and not by convection. Diffusion was homogeneous (nondirectional), consistent with isotropic diffusion. The diffusion rate varied with location along the intestine, correlating with the local FABP concentration and measured cytosolic binding. We conclude that cytoplasmic proteins like FABP promote the intracellular transport of fatty acids by enhancing their diffusive flux. We suggest that facilitation is not specific for a particular cell type but occurs in a variety of cells that transport fatty acids and may contain different types of FABP.  (+info)

Brownian ratchets: molecular separations in lipid bilayers supported on patterned arrays. (7/347)

Brownian ratchets use a time-varying asymmetric potential that can be applied to separate diffusing particles or molecules. A new type of Brownian ratchet, a geometrical Brownian ratchet, has been realized. Charged, fluorescently labeled phospholipids in a two-dimensional fluid bilayer were driven in one direction by an electric field through a two-dimensional periodic array of asymmetric barriers to lateral diffusion fabricated from titanium oxide on silica. Diffusion spreads the phospholipid molecules in the orthogonal direction, and the asymmetric barriers rectify the Brownian motion, causing a directional transport of molecules. The geometrical ratchet can be used as a continuous molecular sieve to separate mixtures of membrane-associated molecules that differ in electrophoretic mobility and diffusion coefficient.  (+info)

Location of the catalytic nucleophile of phospholipase D of Streptomyces antibioticus in the C-terminal half domain. (8/347)

Phospholipase D (PLD) of Streptomyces antibioticus was labelled with fluorescent-labelled substrate, 1-hexanoyl-2-{6-[(7-nitro-2-1, 3-benzoxadiazol-4-yl)-amino]hexanoyl}-sn-glycero-3-phosphocholine, when it was incubated with the substrate and the reaction followed by SDS/PAGE. Mutant enzymes lacking the catalytic activity were not labelled under the same conditions, indicating that labelling of the PLD occurred as the result of its catalytic action. This confirmed that the labelled protein was the phosphatidyl PLD intermediate. PLDs contain two copies of the highly conserved catalytic HxKxxxxD (HKD) motif. Therefore, two protein fragments were separately prepared with recombinant strains of Escherichia coli. One of the fragments was the N-terminal half of the intact PLD containing one HKD motif, and the other was the C-terminal half with the other motif. An active enzyme was reconstructed from these two fragments, and therefore designated fragmentary PLD (fPLD). When fPLD was subjected to the labelling experiment, only the C-terminal half was labelled. Therefore, it was concluded that the catalytic nucleophile that bound directly to the phosphatidyl group of the substrate was located on the C-terminal half of PLD, and that the N-terminal half did not contain such a nucleophile.  (+info)

TY - JOUR. T1 - Selective N-terminal fluorescent labeling of proteins using 4-chloro-7-nitrobenzofurazan. T2 - A method to distinguish protein N-terminal acetylation. AU - Bernal-Perez, Lina F.. AU - Prokai, Laszlo. AU - Ryu, Youngha. PY - 2012/9/1. Y1 - 2012/9/1. N2 - A fluorogenic derivatization method was developed to distinguish the protein N-terminal acetylation status. The unacetylated protein selectively reacted with 4-chloro-7-nitrobenzofurazan (NBD-Cl) at neutral pH to provide high fluorescence. In contrast, the protein with N-terminal acetylation was essentially nonfluorescent under the same conditions despite the presence of many internal lysine residues. Fluorescence of the NBD-labeled protein was very stable, and only micromolar concentrations of proteins were required for reliable detection. This method also provides a general and practical way to quantify proteins when their N-terminal amino group is available.. AB - A fluorogenic derivatization method was developed to distinguish ...
Learn more about 4-chloro-7-nitrobenzofurazan. We enable science by offering product choice, services, process excellence and our people make it happen.
Using brush-border membrane vesicles isolated from calf kidney cortex the effect of tyrosine-reactive reagents on sodium-dependent D-glucose transport was investigated. Treatment of the membranes for 60 min with NBD-Cl (7-chloro-4-nitrobenzo-2-oxa-1,3-diazole), N-acetylimidazole or tetranitromethane decreased D-glucose uptake 50, 70 and 40%, respectively. Tracer exchange experiments revealed that the inhibition of transport is due to a direct modification of the sodium-D-glucose cotransport system. The modification by NBD-Cl decreases the apparent Vmax of the transport system with respect to its interaction with sodium. In addition, the rate of inactivation of the transport system by NBD-Cl is reduced in the presence of high concentrations of sodium. The results indicate that tyrosine residues play an essential role in sodium-D-glucose cotransport and are probably involved in the binding and/or transport of sodium by the sodium-D-glucose cotransport system.
D-Glucose is one of the most important energy sources for the survival of various organisms, from E. colito mammals. For live-cell monitoring of glucose uptake at the single-cell level, a fl uorescent D-glucose derivative 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4- yl)amino]-2-deoxy-D-glucose [2-NBDG], which we developed, has been widely used invarious research fi elds. For the last ten years, however, researchers have awaited an optical control substance forevaluating the extent of non-specifi c adsorption of 2-NBDG upon plasma membrane and/or the rate of unhealthy2-NBDG uptake through partially( or transiently) damaged membrane. Here we introduce a fluorescent L-glucose derivative, 2-[N(- 7-Nitrobenz-2-oxa- 1,3-diazol-4-yl)amino]- 2-deoxy-Lglucose[2-NBDLG]. L-Glucosamine is a key intermediate toward the synthesis of 2-NBDLG, but not commerciallyavailable. Although a few papers on the synthesis of L-glucosamine have been reported, a new synthetic method ofL-glucosamine should be absolutely
I am planning to label some amino acids with NBD-F (4-fluoro-7-nitrobenzofurazan). In some papers Ive read, these derivatives are stated to be very stable. In other papers, they are said to be stable for up to two days when stored in the dark in a refrigerator. Does anyone know what the best storage conditions are? Although this is just a guess, I would think that evaporating the samples to dryness under nitrogen (or with a SpeedVac), and then storing them in a freezer, would increase their storage life. Of course, this assumes that they are more stable when dry, which may not be the case. Anyone know what conditions are most favorable for the preservation/storage of NBD-labeled amino acids? Phil Calvert . -- ------------------------------------ !-----------Phil Calvert-----------! !---calvert.NoSpam at eos.ncsu.edu----! ------------------------------------ NOTE: If present, the phrase .NoSpam must be removed from my return address before sending your reply ...
This study was undertaken to determine whether 4-benzylamino-7-nitrobenz-2-oxa-l,3-diazole (BBD) generates the first excited singlet state of molecular oxygen in liquid solution. The problem was first approached using the ...
Item #: SCP-827. Object Class: Safe. Special Containment Procedures: Site 827 has been established at the location of SCP-827s discovery. For the purposes of the Foundation, SCP-827 has been outfitted with a specialized cell reactor that allows for introduction of samples and removal of their products. Personnel actively interacting with SCP-827 are to wear full Level-C or higher Hazmat gear.. Samples introduced into SCP-827 require approval of project director. Samples from only one individual at a time are to be introduced to ensure there is no genetic cross-contamination. All samples are to be screened for genetic chimerism. In the event that more than one distinct genetic sample is introduced to SCP-827, the sample is to be removed using procedure 827-Hari and incinerated.. Tissue from the central nervous system is not to be used in SCP-827 tests following Incident 827-██.. Description: SCP-827 is a semi-solid mass of biologically active human stem cells. SCP-827 is capable of ...
Learn more about 3-chloro-5-trifluoromethyl-pyridin-2-yloxy-acetic-acid-ethyl-ester. We enable science by offering product choice, services, process excellence and our people make it happen.
Fluorescent glucose biosensors are devices that measure the concentration of glucose in diabetic patients by means of sensitive protein that relays the concentration by means of fluorescence, an alternative to amperometric sension of glucose. No device has yet entered the medical market, but, due to the prevalence of diabetes, it is the prime drive in the construction of fluorescent biosensors. Keeping glucose levels in check is crucial to minimize the onset of the damage caused by diabetes. As a consequence, in conjunction with insulin administrations, the prime requirement for diabetic patients is to regularly monitor their blood glucose levels. The monitoring systems currently in general use have the drawback of below optimal number of readings, due to their reliance on a drop of fresh blood. Some continuous glucose monitors are commercially available, but suffer from the severe drawback of a short working life of the probe. The majority of these work amperometrically. As a result, there is ...
Changes in the spectral properties of plasma membrane lipid analog during the first seconds of endocytosis in living cells, Biophys. , 72, 32-50, 1997. , A novel fluorescent ceramide analog for studying membrane traffic in animal cells; accumulation at the Golgi apparatus results in altered spectral properties of the sphingoid precursor, J. , 113, 1267-1279, 1991. 7 mM), a fluorescent probe of the Golgi apparatus. A leopard-skin appearance of fluorochrome-loaded Golgi sacks is obtained. Similar fluorescence images may be recorded when the cell is treated with fluorescent cytotoxic agents (see Chapter 3). Doctoral dissertation, Carnegie-Mellon University, Pittsburgh, PA, 1990. fm Page 14 Tuesday, November 18, 2003 8:40 AM 14 Atlas of Cell Fluorescence FIGURE 12 Lysosomes with lysososomotropic agent quinacrine. Color image; microphotograph. fm Page 15 Tuesday, November 18, 2003 8:40 AM Vital Fluorescence Probes of Cell Organelles FIGURE 13 Quinacrine imaging of lysosomes. SP micrograph. fm Page 16 ...
Figure 3. Sphingomyelin trafficking and metabolism measured in KB-3-1 and KB-CP.5 cells. A and B, NBD-sphingomyelin labeling of KB-3-1 (A) and KB-CP.5 (B) cells. The cells were labeled with NBD-sphingomyelin at 37° for 1 minute, chased for 30 minutes to achieve steady-state distribution, back exchanged on ice to remove excess cell surface label, and then imaged using wide-field microscopy. Bar, 10 μm. C and D, KB-CP.5 cells double labeled with Alexa 546-transferrin (C) and NBD-sphingomyelin (D). Negligible colocalization is observed. Bar, 10 μm. E, ratio of NBD-ceramide to NBD-sphingomyelin in KB-3-1 cells (black columns) and KB-CP.5 cells (white columns) after excess NBD-sphingomyelin on the plasma membrane has been removed by back exchange. The NBD-lipid ratio thus represents primarily the NBD-lipid proportions in the ERC (because most of the NBD-sphingomyelin from the plasma membranes is removed by the back-exchange procedure). The protocol used for the NBD-lipid ratio assay is explained ...
The sensor was successfully deployed in all five patients and did not interfere with clinical care, blood pressure monitoring or sampling. One patient suffered a cardiopulmonary arrest; the sensor functioned successfully during resuscitation and urgent return to the operating room. One hundred and twenty reference samples ranging from 5.9 to 13.4 mmol/l were collected; 107/120 (89.2%) of GluCath measurements met ISO 15197 criteria (within ±20% of reference when BG ,4.2 mmol/l; Figure 1). In Subject 1 the sensor was inadvertently retracted into the arterial catheter during the study, leading to measurement error from arterial flush solution contamination. In a sensitivity analysis excluding this patient, 89/95 (93.7%) of measurements met ISO 15197 with a mean absolute relative difference of 9.4 ...
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2-NBD-Glucose (186689-07-6) is a fluorescent glucose uptake probe. May be used to monitor glucose uptake in live cells and as a cell viability indi ...
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This work has been made available to the staff and students of the University of Sydney for the purposes of research and study only. It constitutes material that is held by the University for the purposes of reporting for HERDC and the ERA. This work may not be downloaded, copied and distributed to any third party ...
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Item #: SCP-1816. Object Class: Safe. Special Containment Procedures: SCP-1816 is confined in an underground experimental room measuring 10m x 10m x 5m. The room is climate-controlled and equipped with sunlight-simulating lamps. Access to SCP-1816 is forbidden to all female personnel, unless approved by Dr. Coulloudon. The maintenance of SCP-1816, including pruning, fertilization and re-potting, is to be conducted by Professor H. Pak.. Description: SCP-1816 is a penjing containing a live specimen of an unidentified tree. The object is approximately 40 cm high.. SCP-1816 will only affect pregnant mammals. When a subject at an early stage of pregnancy is left in the same room as SCP-1816, the fetus may become an instance of SCP-1816-1. The distance and time of exposure required to cause this effect depends on the size and gestation period of the individual. In the case of murine test subjects, an exposure of 3 minutes/day within 5 meters was found to be sufficient. The requirements for larger ...
Begin Log,. Dr. Hayfield: How long have you been connected to SCP-1884-B?. SCP-1884-A: As long as I can remember. Wherever I have been, Luana has been there with me, even if only in my mind.. Dr. Hayfield: Where did SCP-1884-B come from?. SCP-1884-A: When I was still very young, I asked my mother the same question. She would not tell me. She said she did not want to frighten me.. Dr. Hayfield: Have your blindness and physical abnormalities been present since birth?. SCP-1884-A: Yes. Luana has always been my eyes. She feels the ground so I can walk. She helps me hold things. In my old age, there have been times she has carried me. I am very grateful to her.. Dr. Hayfield: What were the events that led to the incident at the hotel?. SCP-1884-A: That may take some time to explain.. Dr. Hayfield: Thats perfectly fine. Please proceed.. SCP-1884-A: When I was eight years old, men came to our house asking to buy me and Luana. My parents were upset. They always tried to hide us and keep us a secret, ...
5-chloro-2-methyl-4-nitroaniline chemical properties, What are the chemical properties of 5-chloro-2-methyl-4-nitroaniline 13852-51-2, What are the physical properties of 5-chloro-2-methyl-4-nitroaniline ect.
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Experimentation log for SCP-682-2 Item: SCP-053 Termination Test Record: When the subject was first introduced into SCP-053s containment cell, its reaction cou
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ethyl 7-chloro-4-hydroxy-3-quinolinecarboxylate - chemical structural formula, chemical names, chemical properties, synthesis references
We have examined intracellular transport and metabolism of the fluorescent analogue of phosphatidylserine, 1-palmitoyl-2-(N-[12[(7-nitrobenz-2-oxa-1,3-diazole-4-yl)amino] dodecanoyl])-phosphatidylserine ([palmitoyl-C12-NBD]-PS) in cultured fibroblasts. When monolayer cultures were incubated with liposomes containing (palmitoyl-C12-NBD)-PS at 37 degrees C, fluorescent PS was transported to the Golgi apparatus. NBD-containing analogues of phosphatidylcholine, phosphatidylethanolamine (PE), or phosphatidic acid did not accumulate in the Golgi apparatus under the same experimental conditions. We suggest that the transport is not due to endocytosis, but is the result of incorporation and trans-bilayer movement of the (palmitoyl-C12-NBD)-PS at the plasma membrane followed by translocation of the lipid from plasma membrane to the Golgi apparatus via nonvesicular mechanisms. Uptake of fluorescent PS was inhibited by depletion of cellular ATP and was blocked by structural analogues of the lipid or by ...
Materials and cell culture. 1,2-Dioleoyl-sn-glycero-3-phosphocholine, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, N-hexanoyl-d-erythro-sphingosine (C6), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy polyethylene glycol-2000], N-octanoyl-sphingosine-1-[succinyl(methoxy polyethylene glycol-750)] (PEG(750)-C8), N-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-d-erythro-sphingosine (NBD-C6), and N,N-dimethyl-d-erythro-sphingosine (DMSph) were purchased from Avanti Polar Lipids (Alabaster, AL). N-hexanoyl-d-erythro-sphingosine [hexanoyl 6-3H] ([3H]C6) was obtained from ARC (St. Louis, MO). Primary antibodies to caveolin-1 and CD31 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA) and BD PharMingen (San Jose, CA), respectively. Horseradish peroxidase-conjugated goat anti-rabbit IgG and rhodamine red-X-conjugated goat anti-rat IgG secondary antibodies were obtained from Santa Cruz Biotechnology and Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA), ...
You are viewing an interactive 3D depiction of the molecule 2-({6-[(bromoacetyl)amino]hexanoyl}amino)-2-deoxy-d-glucose (C14H25BrN2O7) from the PQR.
The time course of interaction of caldesmon with actin may be monitored by fluorescence changes that occur upon the binding of 12-(N-methyl-N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl))-labeled caldesmon to actin or to acrylodan ...
The time course of interaction of caldesmon with actin may be monitored by fluorescence changes that occur upon the binding of 12-(N-methyl-N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl))-labeled caldesmon to actin or to acrylodan ...
Sigma-Aldrich offers abstracts and full-text articles by [Raiyan T Zaman, Hisanori Kosuge, Guillem Pratx, Colin Carpenter, Lei Xing, Michael V McConnell].
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C07D233/66-Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms ...
Name: 2-Chloro-4-nitrobenzenamine CA Name: Benzenamine,2-chloro-4-nitro- Molecular Structure: 2-Chloro-4-nitrobenzenamine,Benzenamine,2-chloro-4-nitro-,CAS 121-87-9,172.57,C6H5ClN2O2 2-Chloro-4-nitrobenzenamine,Benzenamine,2-chloro-4-nitro-,CAS 121-87-9,172.57,C6H5ClN2O2 Molecular Formula:C6H5ClN2O2 Molecular Weight: 172.57 CAS Registry Number: 121-87-9
Entry Interview for SCP-4409. Interviewer: Dr. Simon Crossley. ,Begin Log,. Dr. Crossley: Good afternoon, SCP-4409, and welcome to Site-66. Im Dr. Crossley, and Ill be conducting both your initial interview and medical exam. Lets see, my boss gave me a list of questions Im supposed to ask you -. SCP-4409: Is the first one why do I look like an elephant?. Dr. Crossley: Ah, its worded a bit more generically than that, but yes. How long has your anomaly been present? Since birth?. SCP-4409: No, I was born as normal as normal can be. This here was done to me, a long time ago. Its not something I relish contemplating, but if you need to hear it I suppose I dont have a choice.. Dr. Crossley: Im all ears. (SCP-4409 glares at Dr. Crossley for several seconds) Ah, my apologies. Im not supposed to antagonize you unnecessarily. Please, tell me what happened to you?. SCP-4409: Ever hear of Herman Fullers Circus of the Disquieting?. Dr. Crossley: Im afraid I havent.. SCP-4409: There are days I ...
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7-chloro-2-ethyl-4-oxo-3,4-dihydro-6-quinazolinesulfonamide - chemical structural formula, chemical names, chemical properties, synthesis references
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Era of polyurethane foam cells, an necessary stage for change cholesterol transportation research, uses the technique of receptor-dependent macrophage launching with radiolabeled acetylated LDL. of lyso-PtdCho Cholesterol was solubilized in PBS by mixing lyso-PtdCho and cholesterol jointly. To determine if lyso-PtdCho could boost the solubility of cholesterol in PBS, continuous quantities of cholesterol, NBD-cholesterol, and an raising quantity of lyso-PtdCho had been used in five cup pipes. After drying out, aqueous solution was produced with 1 ml PBS as defined in Methods and Textiles. Ready blended micelles included 1 millimeter cholesterol (200 d from 5 millimeter share) and 5 Meters NBD-cholesterol (2.5 l from 2 mM stock) and lyso-PtdCho varying from 0C500 M (0, 2.5, 5, 12.5, and 25 d from 20 mM share). Fluorescence strength was motivated in the very clear filtrate. Fluorescence strength of the aqueous option containing only cholesterol and fluorescent cholesterol (but no lyso-PtdCho) was ...
When bovine heart mitochondrial F1-ATPase was labeled with low concentrations of NBD-Cl* in the dark in the presence of ATP at pH 7, the NBD-label was almost exclusively attached to a specific...
The sheath of ,I,Leptothrix cholodnii,/I, is a glycoconjugate composed of a polysaccharide and a peptide rich in cysteine. In this study, structural determination of the hydrazinolyzate of the sheath was carried out. Since the hydrazinolyzate is a polysaccharide incorporated with cysteine, it was ,I,S,/I,-derivatized with a thiol-specific fluorogenic reagent, 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F). Fluorescent fragments were purified by HPLC, and their structures were analyzed by mass spectrometry and NMR spectroscopy. The sheath was found to contain 2-(cysteinyl)amido-2-deoxy-,small,D,/small,-galacturonic acid residue.. ...
TY - JOUR. T1 - Defining raft domains in the plasma membrane. AU - Kusumi, Akihiro. AU - Fujiwara, Takahiro K.. AU - Tsunoyama, Taka A.. AU - Kasai, Rinshi S.. AU - Liu, An An. AU - Hirosawa, Koichiro M.. AU - Kinoshita, Masanao. AU - Matsumori, Nobuaki. AU - Komura, Naoko. AU - Ando, Hiromune. AU - Suzuki, Kenichi G.N.. PY - 2020/1/1. Y1 - 2020/1/1. N2 - Many plasma membrane (PM) functions depend on the cholesterol concentration in the PM in strikingly nonlinear, cooperative ways: fully functional in the presence of physiological cholesterol levels (35~45 mol%), and nonfunctional below 25 mol% cholesterol; namely, still in the presence of high concentrations of cholesterol. This suggests the involvement of cholesterol-based complexes/domains formed cooperatively. In this review, by examining the results obtained by using fluorescent lipid analogs and avoiding the trap of circular logic, often found in the raft literature, we point out the fundamental similarities of liquid-ordered (Lo)-phase ...
2440 We previously reported that EGCG inhibits growth and activation of the EGF receptor (EGFR) and downstream signaling pathways in human colon cancer cells (Shimizu et al., Clin. Cancer Res. 11, 2735-2746, 2005), but the precise mechanism is not known. Several proteins, including EGFR, are believed to partition into detergent-insoluble liquid ordered membrane domains enriched in cholesterol, sphingomyelin, and gangliosides, so-called lipid rafts. Therefore, we examined the effects of EGCG on ordered membrane domains in HT29 colon cancer cells. When the cells were first treated with the fluorescent lipid analog DiIC16,, which preferentially incorporates into ordered domains in the plasma membrane, subsequent treatment with EGCG caused a marked increase the sensitivity of the plasma membrane to extraction by cold Triton X-100, indicating that liquid ordered domains are decreased. Pretreatment with EGCG also inhibited subsequent incorporation of DiIC16 into the plasma membrane, indicating that ...
We report a new 7-nitrobenzo-2-oxa-1, 3-diazolyl (NBD)-based chemosensor containing a piperazine derivative, NBDP, for detection of mercury ions in almost 100% aqueous medium. The chemosensor shows sensing exclusively toward Hg2+ with a switch-on fluorescence response at 543 nm, which could be attributed to the blocking of PET (photo-induced electron transfer) process upon complexation with mercur ...
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Item #: SCP-3068. Object Class: Euclid Keter. Special Containment Procedures: Several long-range Scranton Reality Cannons are trained on SCP-3068. These are to fire upon any containment breaches by SCP-3068-A instances, preventing these instances from existence in ordinary space without the need for costly physical assaults.. It should be noted that Scranton Reality Cannons are an unstable prototype. Their usage is to be restricted to SCP-3068 only. Extreme caution must be observed in their operation.. A diplomatic presence is to be maintained on SCP-3068 at all times. Travel to and from SCP-3068 is to take place using vessels fitted with a Scranton Reality Engine; again, this is an unstable prototype which should only be operated with extreme caution.. Update 09/07/2016: Attempts to locate SCP-3068-B are currently underway. Current protocol is to take SCP-3068-B in for questioning, with the intention of ascertaining its exact link to SCP-3068.. Update 05/10/2017: All use of Scranton Reality ...
Item #: SCP-086. Object Class: Keter. Special Containment Procedures: Item SCP-086 is stored in a heavily soundproofed room, isolated from the rest of the facility by an airlock.. As there is some leakage from SCP-086, the surrounding area is also cordoned off. Object is currently safe, provided no unprotected personnel enter the containment zone, so handling instructions are only provided for the possibility of a security breach, or if needing to move SCP-086.. Unprotected personnel who enter the containment zone should be removed from the area as soon as possible. If they return, or show signs of being compromised by SCP-086, they must be terminated; warnings of these consequences must be displayed prominently around the containment zone.. Description: SCP-086 appears to be a regular tetrahedron approximately 25cm on each side. It constantly emits sounds described as whispering by survivors of its effects, though in no known language.. These sounds are capable of a wide range of effects on ...
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Methyl 5-chloro-6-methylpyrazine-2-carboxylate;77168-85-5;Methyl 5-chloro-6-methylpyrazine-2-carboxylate;ABP001119.Active Biopharma Corp
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5-tetrahydro-8-chloro-3-methyl-5-phenyl-1h-3-benzazepin-7-ol MeSH D03.438.079.800 - 2,3,4,5-tetrahydro-7,8-dihydroxy-1-phenyl- ... 8-chloro-, 2-acetylhydrazide MeSH D03.494.347.500 - loxapine MeSH D03.494.347.500.040 - amoxapine MeSH D03.494.382.393 - ... 4,5-dihydro-1-(3-(trifluoromethyl)phenyl)-1h-pyrazol-3-amine MeSH D03.383.129.539.200 - epirizole MeSH D03.383.129.539.487 - ... 4-oxadiazole MeSH D03.383.312.649.290 - fanft MeSH D03.383.312.649.308 - furagin MeSH D03.383.312.649.313 - furazolidone MeSH ...
It is based on the specificity of the incorporation of the nitrobenzofurazan group, and the ready removal of this group by ... 1. Modification of a single amino acid residue by introduction of the nitrobenzofurazan group inactivates mitochondrial ATPase ... reaction of 4-chloro-7-nitrobenzofurazan with the adenosine triphosphatase of bovine heart submitochondrial particles S J ... reaction of 4-chloro-7-nitrobenzofurazan with the adenosine triphosphatase of bovine heart submitochondrial particles. Biochem ...
4-Chloro-7-nitrobenzofurazan is used as a derivatizing reagent for chromatography analysis of amino acids and low molecular ... It is utilized in the preparation of fluorescent phospholipid-derivative, hydroxynaphthofurazan and 4-chloro-7- ... nitrobenzofurazan- didecanoylphosphatidylethanolamine. It serves as a fluorescent reagent to label free sulfhydryls and N- ... functionalized hydroxynaphthofurazan and 7-nitrobenzofurazan (NBD)-labeled maleimide. ...
Learn more about 4-chloro-7-nitrobenzofurazan. We enable science by offering product choice, services, process excellence and ...
2-Chloro-4-nitrophenol, 97%. ×Close. 2-Chloro-4-nitrophenol, 97% Structure ... 4-(2-Pyridylazo)resorcinol monosodium salt monohydrate. ×Close. 4-(2-Pyridylazo)resorcinol monosodium salt monohydrate ...
Bernal-Perez LF, Prokai L, Ryu Y. Selective N-terminal fluorescent labeling of proteins using 4-chloro-7-nitrobenzofurazan: A ... Bernal-Perez, L. F., Prokai, L., & Ryu, Y. (2012). Selective N-terminal fluorescent labeling of proteins using 4-chloro-7- ... Bernal-Perez, LF, Prokai, L & Ryu, Y 2012, Selective N-terminal fluorescent labeling of proteins using 4-chloro-7- ... nitrobenzofurazan: A method to distinguish protein N-terminal acetylation, Analytical Biochemistry, vol. 428, no. 1, pp. 13-15 ...
7-Methoxycoumarin-3-carboxylic acid N-succinimidyl ester. MeOH. 358. 410. 64958. 2-Methoxy-2,4-diphenyl-3(2H)-furanone. Amine ... 7-Hydroxy-4-methyl-3-coumarinylacetic acid. pH 10.0. 360. 445. 55627. 7-Hydroxy-4-methyl-2(1H)-quinolone. pH 5.0. 321. 357. pH ... 4-Chloro-7-nitrobenzofurazan. MeOH. 336. Aliphatic amine adducts. 475. 540. 29059. 1,3-Cyclohexanedione. Protein adduct. 375. ... 7-(Diethylamino)coumarin-3-carbonyl azide. MeOH. 432. 480. 36799. 7-(Diethylamino)coumarin-3-carboxylic acid. pH 9.0. 409. 473 ...
Novel application of 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole to identify cysteine sulfenic acid in the AhpC component of alkyl ... As shown in Figure 7, only the addition of the oxidized CosR into the Trx system showed a consumption of NADPH. No reduction in ... The formation of Cys-SOH in CosR variants is measured by the 4-chloro-7-nitrobenzofurazan (NBD-Cl) labeling assay [31-33]. ... The formation of disulfide bond and sulfenic acid in CosR WT and its variants are performed with the thiol-reactive probe 4- ...
2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose ...
Masturah Bte Mohd Abdul Rashid 1 , Tan Boon Toh 2 , Aleidy Silva 3 , Lissa Nurrul Abdullah 2 , Chih-Ming Ho 3 , Dean Ho 4 , ... 4 Division of Oral Biology and Medicine, Division of Advanced Prosthodontics, The Jane and Jerry Weintraub Center for ... Specifically, we showed that a fluorescent 2-deoxyglucose analog, 2-[N-(7-nitrobenz-2- oxa-1,3-diazol-4-yl)amino]-2- ... 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose ...
Description: the structure of bovine f1-ATPase covalently inhibited with 4-chloro-7-nitrobenzofurazan. Class: ATP synthase. ... Keywords: ATP synthase, f1fo ATP synthase, f1-ATPase, 4-chloro-7-nitrobenzofurazan, inhibition. Deposited on 1998-04-30, ...
4 A and D, and of 4ASU for Fig. 4B that were simplified from SI Appendix, Fig. S1 to emphasize mechanistic features. Several ... 4). As the catalytic dwell ends (Fig. 4A), catalytic site βD hydrolyzed ATP to Pi and ADP. Behind subunit γ, catalytic site βT ... 4, ) to enable tight winding that can store elastic energy. In all F1 structures known to date, subunit γ is tethered to the ( ... 4C) is thought to result from force applied to subunit γ as a crankshaft from ATP binding-dependent closure βE-lever (i.e., the ...
Cells were labeled with 4 μM C6NBD-GlcCer or C6-NBD-SM at 37°C for 30 min. ... Cells were labeled with 4 μM C6NBD-GlcCer or C6-NBD-SM at 37°C for 30 min. Both lipid analogues were present in the BC ( ... Cells were labeled with 4 μM C6NBD-GlcCer or C6-NBD-SM at 37°C for 30 min. Both lipid analogues were present in the BC ( ... As a control, cells were incubated in HBSS in the absence of sodiumdithionite at 4°C for 7 min. After extensively washing (15 ...
Description: the structure of bovine f1-atpase covalently inhibited with 4-chloro-7-nitrobenzofurazan. Deposited on 1998-04-30 ...
4 (a) SEM micrograph of the anode non-woven carbon mat coated with PVFc CNT with details of coated and uncoated areas. (b and c ... Figure 4. XRD pattern of ash mixed with MS and SS under different conditions (a: MS; b: SS; c: M9S1; and d: M8S2). (1) SiO2, (2 ... Figure 7. Scatter plots of the cell performance against αCO, xCO2in, and L in (a c) the IND scenario (circles) and (d f) the ... Table 4: For the next few graphics, there is a parameter called ICP for Indicator of Comprehensive Performance. There are ...
Quantitative analysis of sertraline in human serum by LC with fluorescence detection after pre-column derivatization with 4- ... chloro-7-nitrobenzofurazan. Bahrami, G; Mohammadi, B; Farshchi, A; Ghiasi, G. * Development of a sensitive liquid-liquid ...
Similarly, chloro drug derivative of diltiazem was formed by means of reaction involving tertiary amino group of diltiazem and ... the 8-chloro derivative of diltiazem (clentiazem maleate) and their deacetyl forms were resolved on ovomucoid-bonded chiral ... "Direct high-performance liquid chromatographic separation of the enantiomers of diltiazem hydrochloride and its 8-chloro ... Nighat Shafi,1 Farhan Ahmed Siddiqui,1 Huma Naseem,2 Nawab Sher,3 Arif Zubair,4 Azhar Hussain,5 Ali Akbar Sial,1 and Mirza ...
4, pp. 763-770, 2008. View at: Publisher Site , Google Scholar*S. M. R. Wille, P. van Hee, H. M. Neels, C. H. van Peteghem, and ... 4, pp. 345-349, 2005. View at: Publisher Site , Google Scholar*C. Fernandes, P. Jiayu, P. Sandra, and F. M. Lanças, "Stir bar ... 4, pp. 955-962, 2007. View at: Publisher Site , Google Scholar*V. F. Samanidou and P. V. Kourti, "Rapid HPLC method for the ... 4, pp. 467-473, 2008. View at: Publisher Site , Google Scholar*H. Zhu, J. Luo, G. Zheng, and J. Shentu, "Sensitive and specific ...
5-tetrahydro-8-chloro-3-methyl-5-phenyl-1h-3-benzazepin-7-ol MeSH D03.438.079.800 - 2,3,4,5-tetrahydro-7,8-dihydroxy-1-phenyl- ... 8-chloro-, 2-acetylhydrazide MeSH D03.494.347.500 - loxapine MeSH D03.494.347.500.040 - amoxapine MeSH D03.494.382.393 - ... 4,5-dihydro-1-(3-(trifluoromethyl)phenyl)-1h-pyrazol-3-amine MeSH D03.383.129.539.200 - epirizole MeSH D03.383.129.539.487 - ... 4-oxadiazole MeSH D03.383.312.649.290 - fanft MeSH D03.383.312.649.308 - furagin MeSH D03.383.312.649.313 - furazolidone MeSH ...
CAS: 4197-24-4 Molecular Formula: C21H22ClN3 Molecular Weight (g/mol): 351.878 MDL Number: MFCD00143923 InChI Key: ... 4-Chloro-7-nitrobenzofurazan, 4-Chloro-7-nitrobenzo-2-oxa-1, 3-diazole PubChem CID: 25043 ChEBI: CHEBI:78878 ... 7-Hydroxy-3H-phenoxazin-3-one 10-oxide, sodium salt PubChem CID: 112939 ...
4. The variations in I50 values for efrapeptin closely followed changes in specific activity of ATPase, as would be expected ... 4-chloro-7-nitrobenzofurazan, quercetin and spegazzinine, all of which show different sensitivity profiles from one another. 5 ...
FIG 4 B22 enters the cytoplasm and induces membrane blebbing in regions of active peptidoglycan turnover. (A) Time-lapse phase- ... Initial attachment (no flow) proceeded for 30 min followed by a flow rate of 0.5 ml min−1 for 4 h in medium containing a 20,000 ... FIG 7 B22 induces oxidative stress, cell shortening, and membrane blebbing. (A) Time-lapse phase-contrast and fluorescent image ... One milliliter of culture was centrifuged at 14,000 × g for 5 min at 4°C. Cell pellets were washed once with cold medium, ...
4 CRL Guidance Paper . -CRLs view on state of the art analytical methods for the national residue control plans; ( 2007 ); http ... 4 CRL Guidance Paper . -CRLs view on state of the art analytical methods for the national residue control plans; ( 2007 ); http ... Equipment and instruments Kinetex XB C-18 HPLC column (100 mm × 3 mm, 2.6 μm) and C-18 security guard column (4 mm × 2 mm); ... Equipment and instruments Kinetex XB C-18 HPLC column (100 mm × 3 mm, 2.6 μm) and C-18 security guard column (4 mm × 2 mm); ...
GLUTATHIONE-S-TRANSFERA 1-Chloro-2,4-Dinitrobenzene as SubstrateSE -Enzymatic Assay Protocol ...
4-Chloro-7-nitrobenzofurazan - Preferred Concept UI. M0014530. Scope note. A benzofuran derivative used as a protein reagent ... 4-Chloro-7-nitrobenzofurazan Entry term(s). 4 Chloro 7 nitrobenzofurazan 7 Chloro 4 nitrobenzofurazan 7-Chloro-4- ... nitrobenzofurazan Chloride, NBD Chloride, Nitrobenzoxadiazole Chloronitrobenzoxadiazole NBD Chloride NBF Cl NBF-Cl ... 4-Chloro-7-nitro-2,1,3-benzoxadiazole Entry term(s):. 4 Chloro 7 nitrobenzofurazan. 7 Chloro 4 nitrobenzofurazan. 7-Chloro-4- ...
UNITED STATES AGENCY FOR HEALTH CARE POLICY AND RESEARCH ...
Prokai, L., Guo, J. & Prokai-Tatrai, K., 1 Jan 2014, In : Nature Protocols. 9, 4, p. 882-895 14 p.. Research output: ... Nguyen, V., Zharikova, A. D., Prokai-Tatrai, K. & Prokai, L., 1 Apr 2010, In : Brain Research Bulletin. 82, 1-2, p. 83-86 4 p. ... Rauniyar, N., Prokai-Tatrai, K. & Prokai, L., 1 Apr 2010, In : Journal of Mass Spectrometry. 45, 4, p. 398-410 13 p.. Research ... Prokai, L., Szarka, S., Wang, X. & Prokai-Tatrai, K., 6 Apr 2012, In : Journal of Chromatography A. 1232, p. 281-287 7 p.. ...
We show that N-[N-(7-nitro-2,1,3-benzoxadiazol-4-yl)-epsilon-aminohexanoyl]- sphingosylphosphorylcholine (C6-NBD-SM) in ...
During repetitive stimulation of skeletal muscle, extracellular ATP levels raise, activating purinergic receptors, increasing Ca2+ influx, and enhancing contractile force, a response called potentiation. We found that ATP appears to be released through pannexin1 hemichannels (Panx1 HCs). Immunocytochemical analyses and function were consistent with pannexin1 localization to T-tubules intercalated with dihydropyridine and ryanodine receptors in slow (soleus) and fast (extensor digitorum longus, EDL) muscles. Isolated myofibers took up ethidium (Etd+) and released small molecules (as ATP) during electrical stimulation. Consistent with two glucose uptake pathways, induced uptake of 2-NBDG, a fluorescent glucose derivative, was decreased by inhibition of HCs or glucose transporter (GLUT4), and blocked by dual blockade. Adult skeletal muscles apparently do not express connexins, making it unlikely that connexin hemichannels contribute to the uptake and release of small molecules. ATP release, Etd+ ...
  • An accurate and sensitive reversed-phase high-performance liquid chromatograph-ic method for determination of sertraline in human serum is described using 4-chloro-7-nitrobenzofurazan as pre-column derivatization agent. (ijps.ir)
  • For this, the cells were labeled with fluorescent acyl chain-labeled 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-amino]hexanoic acid (C6-NBD) derivatives of either GlcCer (C6-NBD-GlcCer) or SM (C6-NBD-SM). (nih.gov)
  • 1. Modification of a single amino acid residue by introduction of the nitrobenzofurazan group inactivates mitochondrial ATPase (adenosine triphosphatase) when membrane-bound in submitochondrial particles. (portlandpress.com)
  • The similarity between the reactions of both membrane-bound and isolated ATPase with 4-chloro-7-nitrobenzofurazan indicates that the single essential tryosine residue identified in the isolated enzyme [Ferguson, Loyd, Lyons & Radda (1975) Eur. (portlandpress.com)
  • Using inhibitors of known action, such as the H+ ATPase inhibitors NBD-Cl and NEM, the Ca2+ ATPase inhibitor vanadate as well as the second messenger inositol 1,4,5-trisphosphate (IP3) and its analog inositol 1,4,5-trisphosphorothioate (IPS3), we could functionally differentiate two nonmitochondrial Ca2+ pools. (ox.ac.uk)
  • Both methods are based on coupling with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in borate buffer of pH 7.8 and measuring the reaction product spectrophotometrically at 395 nm (Method I) or spectrofluorimetrically at 530 nm upon excitation at 480 nm (Method II). (biomedcentral.com)
  • The present study was aimed to develop an accurate and sensitive method to measure sertraline in human serum, using 4-chloro-7-nitrobenzofurazan (NBD-Cl) as labeling agent and test its applicability for analysis of the drug in a healthy volunteers following single dose administration. (ijps.ir)
  • In addition it is used in the synthesis of fluorescent phospholipid-derivative, NBD-didecanoylphosphatidylethanolamine, functionalized hydroxynaphthofurazan and 7-nitrobenzofurazan (NBD)-labeled maleimide. (alfa.com)
  • Cathelicidins are thought to interact with each other within the membranes of susceptible bacteria to form assemblages that affect permeability and result in cell death ( 4 ). (asm.org)
  • The fluorescent emission spectra of the 7-nitrobenz-2-oxa-1,3-diazole-4-yl (NBD)-labelled alpha-5 peptides displayed a blue shift upon binding to insect (Spodoptera littoralis) mid-gut membranes, reflecting the relocation of the fluorescent probes to an environment of increased apolarity, i.e. within the lipidic constituent of the membrane. (ox.ac.uk)
  • Bernal-Perez, LF , Prokai, L & Ryu, Y 2012, ' Selective N-terminal fluorescent labeling of proteins using 4-chloro-7-nitrobenzofurazan: A method to distinguish protein N-terminal acetylation ', Analytical Biochemistry , vol. 428, no. 1, pp. 13-15. (unthsc.edu)
  • The method is based on the interaction between D-penicillamine in aqueous solution and 4-chloro-7-nitro-benzofurazan [NBD-C1], 0.1% w/v in methanol , in presence of 0.1 M borax solution. (bvsalud.org)
  • Characterization of inositol 1,4,5-trisphosphate-sensitive (IsCaP) and -insensitive (IisCaP) nonmitochondrial Ca2+ pools in rat pancreatic acinar cells. (ox.ac.uk)
  • C. glutamicum cells are frequently confronted with excessive reactive oxygen species (ROS) production, triggering sudden changes of fermentative conditions in temperature, pH, osmotic pressure, or toxic compounds [ 4 - 5 ]. (portlandpress.com)
  • Specifically, we showed that a fluorescent 2-deoxyglucose analog, 2-[N-(7-nitrobenz-2- oxa-1,3-diazol-4-yl)amino]-2-deoxyglucose, and CellTrace Calcein Red-Orange AM can be used reliably as readouts for glucose uptake and proliferative index, respectively, to identify drug candidates that simultaneously reduce glucose uptake and induce cell death in HCC cells. (nih.gov)
  • Cells were labeled with 4 μM C6NBD-GlcCer or C6-NBD-SM at 37°C for 30 min. (nih.gov)
  • Note that in order to visualize intracellular fluorescence in b and d, basolateral membrane-located lipid analogues were partly removed by a brief back exchange procedure (5-10 min, 4°C). Alternatively, cells incubated as in a and c were subsequently subjected to a back exchange procedure at 4°C for 30 min to completely remove the basolateral PM pool of lipid analogues. (nih.gov)
  • After back exchange, the cells were incubated in HBSS, supplemented with 30 mM sodiumdithionite at 4°C for 7 min. (nih.gov)
  • 4-Chloro-7-nitrobenzofurazan is used as a derivatizing reagent for chromatography analysis of amino acids and low molecular weight amines. (alfa.com)
  • Addition of sulfhydryl groups to Escherichia coli ribosomes by protein modification with 2-iminothiolane (methyl 4-mercaptobutyrimidate). (naver.com)
  • Sertraline hydrochloride [(cis-(1S,4S)-N-methyl-4 (3,4-dichlorophenyl)-1,2,3,4-tetrahydro-1 naphthalenamine)] is a relatively new selective serotonin reuptake inhibitor (SSRI) which is administrated in treatment of depression, panic disorder, anxiety and social phobia [1]. (ijps.ir)
  • It is utilized in the preparation of fluorescent phospholipid-derivative, hydroxynaphthofurazan and 4-chloro-7-nitrobenzofurazan- didecanoylphosphatidylethanolamine. (alfa.com)
  • The unacetylated protein selectively reacted with 4-chloro-7-nitrobenzofurazan (NBD-Cl) at neutral pH to provide high fluorescence. (unthsc.edu)
  • Diltiazem hydrochloride (DLZ), d-cis diltiazem, d-cis-3-acetyloxy-5-[2-(dimethylamino) ethyl]-2,3-dihydro-2-(4-methoxyphenyl)-1,5-benzothiazepin-4(5H)one)hydrochloride (Figure 1 ) are one of the widely used benzothiazepine Ca 2+ -blocking drugs (calcium channel blockers). (hindawi.com)
  • Photophosphorylation in vivo by Chlorobium limicola was inhibited by lipophilic cations and the energy-transfer inhibitors diphenylphosphorylazide, Dio-9, 4-chloro-7-nitrobenzofurazan and chlorhexidene. (microbiologyresearch.org)
  • The sensitivity of the processes producing this membrane potential to uncouplers, energy-transfer inhibitors and 2-heptyl-4-hydroxyquinoline- N -oxide was measured in the light and the dark. (microbiologyresearch.org)
  • Two inhibitors of energy conservation- N,N′ -dicyclohexylcarbodi-imide and 4-chloro-7-nitrobenzofurazan-inhibited respiration maximally at 00 and 06 of a cycle whilst an inhibitor of respiration-2-heptyl-4-hydroxyquinoline- N -oxide-exerted maximal effect only at 04 of a cycle. (microbiologyresearch.org)
  • Histamine H 2 receptor antagonists: cimetidine (CIM), ranitidine (RAN), and famotidine (FAM) classified as class III drugs (high solubility, low permeability) according to the Biopharmaceutics Classification System (BCS) [ 3 , 4 ] are used in the treatment of gastrooesophageal reflux disease and gastric and duodenal ulceration [ 5 ]. (hindawi.com)
  • This methods, however, are not sensitive enough to measure low drug levels which are obtained in human single dose studies [3-5, 7], or need an expensive instrument which is not available in many laboratories [6-9, 11]. (ijps.ir)
  • below pH 7, they form brittle, continuous sheets, and at pH 8, they form lace-like networks that deform plastically under shear. (sigmaaldrich.com)
  • This MRPL was changed to 10 μg kg−1 as a recommended concentration (RC) in 2007 (3, 4). (deepdyve.com)
  • Regression analysis of Beer 's plot showed good correlation [r=0.9990] in a general concentration range of 4-20 micro g ml-1 with a detection limit of 0.078, which represent the minimum absorbance value that can be measured. (bvsalud.org)
  • The drug is slowly absorbed with time to peak plasma concentration of about 4-8 h and elimination half life of 22-35 h. (ijps.ir)
  • The new generation antidepressant drugs are the most widespread class of drug and are in fact becoming the drugs of first choice for the treatment of depression, because they are considered to be more potent than other antidepressant groups [ 5 , 7 ]. (hindawi.com)
  • these fluctuations were different from those for aurovertin, Dio-9, 4-chloro-7-nitrobenzofurazan, quercetin and spegazzinine, all of which show different sensitivity profiles from one another. (biochemj.org)
  • We show that N-[N-(7-nitro-2,1,3-benzoxadiazol-4-yl)-epsilon-aminohexanoyl]- sphingosylphosphorylcholine (C6-NBD-SM) in endocytosed as bulk membrane, and it transits the endocytic system kinetically and morphologically identically to fluorescently labeled transferrin in a CHO cell line. (mblwhoilibrary.org)
  • It is based on the specificity of the incorporation of the nitrobenzofurazan group, and the ready removal of this group by compounds that contain a thiol group. (portlandpress.com)