4-Chloro-7-nitrobenzofurazan: A benzofuran derivative used as a protein reagent since the terminal N-NBD-protein conjugate possesses interesting fluorescence and spectral properties. It has also been used as a covalent inhibitor of both beef heart mitochondrial ATPase and bacterial ATPase.OxadiazolesBenzoxazolesAurovertins: Very toxic and complex pyrone derivatives from the fungus Calcarisporium arbuscula. They bind to and inhibit mitochondrial ATPase, thereby uncoupling oxidative phosphorylation. They are used as biochemical tools.Ficain: A sulfhydryl proteinase with cysteine at the active site from ficus latex. Preferential cleavage is at tyrosine and phenylalanine residues. EC 3.4.22.3.Benzofurans: Compounds that contain a BENZENE ring fused to a furan ring.Proton-Translocating ATPases: Multisubunit enzymes that reversibly synthesize ADENOSINE TRIPHOSPHATE. They are coupled to the transport of protons across a membrane.Chemistry: A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.Chemical Phenomena: The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.Mitochondria, Heart: The mitochondria of the myocardium.2-Chloroadenosine: 2-Chloroadenosine. A metabolically stable analog of adenosine which acts as an adenosine receptor agonist. The compound has a potent effect on the peripheral and central nervous system.Halogens: A family of nonmetallic, generally electronegative, elements that form group 17 (formerly group VIIa) of the periodic table.Sulfhydryl Compounds: Compounds containing the -SH radical.ChlorobenzenesBenztropine: A centrally active muscarinic antagonist that has been used in the symptomatic treatment of PARKINSON DISEASE. Benztropine also inhibits the uptake of dopamine.Kinetics: The rate dynamics in chemical or physical systems.Lysine: An essential amino acid. It is often added to animal feed.Structure-Activity Relationship: The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Chlorine: A greenish-yellow, diatomic gas that is a member of the halogen family of elements. It has the atomic symbol Cl, atomic number 17, and atomic weight 70.906. It is a powerful irritant that can cause fatal pulmonary edema. Chlorine is used in manufacturing, as a reagent in synthetic chemistry, for water purification, and in the production of chlorinated lime, which is used in fabric bleaching.Catechols: A group of 1,2-benzenediols that contain the general formula R-C6H5O2.Biodegradation, Environmental: Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.Stereoisomerism: The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Molecular Structure: The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.Magnetic Resonance Spectroscopy: Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
(1/347) Specific and sensitive assay for alkaline and neutral ceramidases involving C12-NBD-ceramide.

A fluorescent analogue of ceramide, C12-NBD-ceramide, was found to be hydrolyzed much faster than 14C-labeled ceramide by alkaline ceramidase from Pseudomonas aeruginosa and neutral ceramidase from mouse liver, while this substrate was relatively resistant to acid ceramidase from plasma of the horseshoe crab. The radioactive substrate was used more preferentially by the acid ceramidase. It should be noted that C6-NBD-ceramide, which is usually used for ceramidase assays, was hardly hydrolyzed by any of the enzymes examined, compared to C12-NBD-ceramide. For the alkaline and neutral enzymes, the Vmax and k (Vmax/Km) with C12-NBD-ceramide were much higher than those with 14C-ceramide. In contrast, for the acid enzyme these parameters with C12-NBD-ceramide were less than half those with the radioisotope-labeled substrate. It is noteworthy that the labeling of ceramide with NBD did not itself reduce the Km of the alkaline enzyme, but did that of the neutral enzyme. It was also found that C12-NBD-ceramide was preferentially hydrolyzed by the alkaline and neutral enzymes, but not the acid one, in several mammalian cell lines. This study clearly shows that the attachment of NBD, but not dansyl, increases the susceptibility of ceramide to alkaline and neutral enzyme, and decreases that to acid enzymes. Thus the use of this substrate provides a specific and sensitive assay for alkaline and neutral ceramidases.  (+info)

(2/347) Maturation of the axonal plasma membrane requires upregulation of sphingomyelin synthesis and formation of protein-lipid complexes.

Neuronal maturation is a gradual process; first axons and dendrites are established as distinct morphological entities; next the different intracellular organization of these processes occurs; and finally the specialized plasma membrane domains of these two compartments are formed. Only when this has been accomplished does proper neuronal function take place. In this work we present evidence that the correct distribution of a class of axonal membrane proteins requires a mechanism which involves formation of protein-lipid (sphingomyelin/cholesterol) detergent-insoluble complexes (DIGs). Using biochemistry and immunofluorescence microscopy we now show that in developing neurons the randomly distributed Thy-1 does not interact with lipids into DIGs (in fully developed neurons the formation of such complexes is essential for the correct axonal targeting of this protein). Using lipid mass spectrometry and thin layer chromatography we show that the DIG lipid missing in the developing neurons is sphingomyelin, but not cholesterol or glucosylceramide. Finally, by increasing the intracellular levels of sphingomyelin in the young neurons the formation of Thy-1/DIGs was induced and, consistent with a role in sorting, proper axonal distribution was facilitated. These results emphasize the role of sphingomyelin in axonal, and therefore, neuronal maturation.  (+info)

(3/347) Saturable stimulation of fatty acid transport through model cytoplasm by soluble binding protein.

To better define the role of soluble binding proteins in the cytoplasmic transport of amphipathic molecules, we measured the diffusional mobility of a fluorescent long-chain fatty acid, 12-N-methyl-(7-nitrobenz-2-oxa-1,3-diazol)aminostearate (NBD-stearate), through model cytoplasm as a function of soluble binding protein concentration. Diffusional mobilities were correlated with the partition of the fatty acid between membrane and protein binding sites. Cytoplasm was modeled as a dense suspension of liposomes, and albumin was used as a model binding protein. Albumin saturably increased NBD-stearate mobility through the membrane suspension approximately eightfold. Fatty acid mobility in the absence of albumin was identical to the mobility of the membrane vesicles (1.99 +/- 0.33 x 10(-8) cm(2)/s), whereas the mobility at saturating concentrations was identical to the mobility of albumin (1.65 +/- 0.12 x 10(-7) cm(2)/s). The protein concentration producing half-maximal stimulation of NBD-stearate diffusion (42.8 +/- 0.3 microM) was unexpectedly greater than that required to solubilize half of the NBD-stearate (17.9 +/- 3.0 microM). These results support a proposed mechanism for cytoplasmic transport of small amphipathic molecules in which aqueous diffusion of the protein-bound form of the molecule largely determines the transport rate. However, slow interchange of fatty acid between the binding protein and membranes also appears to influence the transport rate in this model system.  (+info)

(4/347) Fluorescent phosphoinositide derivatives reveal specific binding of gelsolin and other actin regulatory proteins to mixed lipid bilayers.

Fluorescent derivatives of phosphatidyl inositol (PtdIns)-(4,5)-P2 were synthesized and used to test the effects of the PtdIns-(4, 5)-P2-regulated proteins gelsolin, tau, cofilin, and profilin on labeled PtdIns-(4,5)-P2 that was either in micellar form or mixed with phosphatidylcholine (PtdCho) in bilayer vesicles. Gelsolin increased the fluorescence of 7-nitrobenz-2-oxa-1,3-diazole (NBD)- or pyrene-labeled PtdIns-(4,5)-P2 and NBD-PtdIns-(3,4,5)-P3. Cofilin and profilin produced no detectable change at equimolar ratios to PtdIns-(4,5)-P2, while tau decreased NBD-PtdIns-(4,5)-P2 fluorescence. Fluorescence enhancement by gelsolin of NBD-PtdIns-(4, 5)-P2 in mixed lipid vesicles depended on the mole fraction of PtdIns-(4,5)-P2 in the bilayer. Specific enhancement of 3% NBD-PtdIns-(4,5)-P2 : 97% PtdCho was much lower than that of 10% PtdIns-(4,5)-P2 : 90% PtdCho, but the enhancement of 3% NBD-PtdIns-(4,5)-P2 could be increased by addition of 7% unlabeled PtdIns-(4,5)-P2. The gelsolin-dependent increase in NBD-PtdIns-(4, 5)-P2 fluorescence was reversed by addition of Ca2+ or G-actin. Significant, but weaker, fluorescence enhancement was observed with the gelsolin N-terminal domain (residues 1-160) and a peptide comprised of gelsolin residues 150-169. Fluorescence energy transfer from gelsolin to pyrene-PtdIns-(4,5)-P2 was much stronger with intact gelsolin than the N-terminal region of gelsolin containing the PtdIns-(4,5)-P2 binding sites, suggesting that PtdIns-(4,5)-P2 may bind near a site formed by the juxtaposition of the N- and C-terminal domains of gelsolin.  (+info)

(5/347) Rapid transbilayer movement of fluorescent phospholipid analogues in the plasma membrane of endocytosis-deficient yeast cells does not require the Drs2 protein.

Evidence is presented that endocytosis-deficient Saccharomyces cerevisiae end4 yeast cells rapidly internalize the fluorescent phospholipid analogues 1-palmitoyl-2-{6-[7-nitro-2,1, 3-benzoxadiazol-4-yl(NBD)amino] caproyl}phosphatidylcholine (P-C6-NBD-PtdCho) and P-C6-NBD-phosphatidylserine (P-C6-NBD-PtdSer). Both analogues redistributed between the exoplasmic and cytoplasmic leaflet with a half-time of < 15 min at 0 degrees C. The plateau of internalized analogues was about 70%. Transbilayer movement is probably protein-mediated, as the flip-flop of both analogues was very slow in liposomes composed of plasma-membrane lipids. Rapid analogue internalization was not abolished on depletion of intracellular ATP by about 90%. For P-C6-NBD-PtdCho only was a moderate decrease in the plateau of internalized analogues of about 20% observed, while that of P-C6-NBD-PtdSer was not affected. The Drs2 protein plays only a minor role, if any, in the rapid transbilayer movement of analogues in S. cerevisiae end4 cells. In S. cerevisiae end4 Deltadrs2 cells harbouring both an end4 allele and a drs2 null allele, about 60% and 50% of P-C6-NBD-PtdCho and P-C6-NBD-PtdSer, respectively, became internalized within 15 min at 0 degrees C. The preferential orientation of P-C6-NBD-PtdSer to the cytoplasmic leaflet is in qualitative agreement with the sequestering of endogenous phosphatidylserine to the cytoplasmic leaflet, as assessed by binding of annexin V. Virtually no binding of annexin V to spheroplasts of the parent wild-type strain or the mutant strains was observed. Likewise, no difference in the exposure of endogenous aminophospholipids to the exoplasmic leaflet between these strains was found by labelling with trinitrobenzenesulfonic acid. Thus, lipid asymmetry, at least of aminophospholipids, was preserved in S. cerevisiae end4 cells independently of the presence of the Drs2 protein.  (+info)

(6/347) Cytoplasmic transport of fatty acids in rat enterocytes: role of binding to fatty acid-binding protein.

The intracellular movement of fatty acids is thought to be facilitated through codiffusion with fatty acid-binding protein (FABP). This facilitation may occur by decreasing binding to immobile membranes, leading to faster cytoplasmic diffusion. The aims of this study were to measure the intracellular transport of 12-N-methyl-(7-nitrobenzo-2-oxa-1,3-diazol)aminostearate (NBD-stearate) in villus rat enterocytes and to determine 1) the mechanism of its cytoplasmic transport and 2) if its transport rate correlated with the known variation of FABP binding capacity along the length of the small intestine. Two-dimensional laser photobleaching was used to measure the movement of a fluorescent fatty acid NBD-stearate in enterocytes isolated from different segments of rat intestine. The fraction of NBD-stearate found in the cytostol of enterocytes was determined by differential centrifugation. Cytoplasmic transport of NBD-stearate occurred solely by diffusion and not by convection. Diffusion was homogeneous (nondirectional), consistent with isotropic diffusion. The diffusion rate varied with location along the intestine, correlating with the local FABP concentration and measured cytosolic binding. We conclude that cytoplasmic proteins like FABP promote the intracellular transport of fatty acids by enhancing their diffusive flux. We suggest that facilitation is not specific for a particular cell type but occurs in a variety of cells that transport fatty acids and may contain different types of FABP.  (+info)

(7/347) Brownian ratchets: molecular separations in lipid bilayers supported on patterned arrays.

Brownian ratchets use a time-varying asymmetric potential that can be applied to separate diffusing particles or molecules. A new type of Brownian ratchet, a geometrical Brownian ratchet, has been realized. Charged, fluorescently labeled phospholipids in a two-dimensional fluid bilayer were driven in one direction by an electric field through a two-dimensional periodic array of asymmetric barriers to lateral diffusion fabricated from titanium oxide on silica. Diffusion spreads the phospholipid molecules in the orthogonal direction, and the asymmetric barriers rectify the Brownian motion, causing a directional transport of molecules. The geometrical ratchet can be used as a continuous molecular sieve to separate mixtures of membrane-associated molecules that differ in electrophoretic mobility and diffusion coefficient.  (+info)

(8/347) Location of the catalytic nucleophile of phospholipase D of Streptomyces antibioticus in the C-terminal half domain.

Phospholipase D (PLD) of Streptomyces antibioticus was labelled with fluorescent-labelled substrate, 1-hexanoyl-2-{6-[(7-nitro-2-1, 3-benzoxadiazol-4-yl)-amino]hexanoyl}-sn-glycero-3-phosphocholine, when it was incubated with the substrate and the reaction followed by SDS/PAGE. Mutant enzymes lacking the catalytic activity were not labelled under the same conditions, indicating that labelling of the PLD occurred as the result of its catalytic action. This confirmed that the labelled protein was the phosphatidyl PLD intermediate. PLDs contain two copies of the highly conserved catalytic HxKxxxxD (HKD) motif. Therefore, two protein fragments were separately prepared with recombinant strains of Escherichia coli. One of the fragments was the N-terminal half of the intact PLD containing one HKD motif, and the other was the C-terminal half with the other motif. An active enzyme was reconstructed from these two fragments, and therefore designated fragmentary PLD (fPLD). When fPLD was subjected to the labelling experiment, only the C-terminal half was labelled. Therefore, it was concluded that the catalytic nucleophile that bound directly to the phosphatidyl group of the substrate was located on the C-terminal half of PLD, and that the N-terminal half did not contain such a nucleophile.  (+info)

*  List of MeSH codes (D03)
5-tetrahydro-8-chloro-3-methyl-5-phenyl-1h-3-benzazepin-7-ol MeSH D03.438.079.800 --- 2,3,4,5-tetrahydro-7,8-dihydroxy-1-phenyl ... 8-chloro-, 2-acetylhydrazide MeSH D03.494.347.500 --- loxapine MeSH D03.494.347.500.040 --- amoxapine MeSH D03.494.382.393 --- ... 4,5-dihydro-1-(3-(trifluoromethyl)phenyl)-1h-pyrazol-3-amine MeSH D03.383.129.539.200 --- epirizole MeSH D03.383.129.539.487 ... 4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, methyl ester MeSH D03.383.725.210 --- dimethindene MeSH D03.383. ...
10199-89-0 - 4-Chloro-7-nitrobenzofurazan, 99% - 4-Chloro-7-nitrobenzo-2,1,3-oxadiazole - NBD chloride - A14165 - Alfa Aesar  10199-89-0 - 4-Chloro-7-nitrobenzofurazan, 99% - 4-Chloro-7-nitrobenzo-2,1,3-oxadiazole - NBD chloride - A14165 - Alfa Aesar
4-Chloro-7-nitrobenzofurazan is used as a derivatizing reagent for chromatography analysis of amino acids and low molecular ... It is utilized in the preparation of fluorescent phospholipid-derivative, hydroxynaphthofurazan and 4-chloro-7- ... nitrobenzofurazan- didecanoylphosphatidylethanolamine. It serves as a fluorescent reagent to label free sulfhydryls and N- ... functionalized hydroxynaphthofurazan and 7-nitrobenzofurazan (NBD)-labeled maleimide. ...
more infohttps://www.alfa.com/de/catalog/A14165/
4-chloro-7-nitrobenzofurazan | VWR  4-chloro-7-nitrobenzofurazan | VWR
Learn more about 4-chloro-7-nitrobenzofurazan. We enable science by offering product choice, services, process excellence and ...
more infohttps://us.vwr.com/store/product/7674484/4-chloro-7-nitrobenzofurazan
Indicators and Dyes for Chemical Processing - Alfa Aesar  Indicators and Dyes for Chemical Processing - Alfa Aesar
2-Chloro-4-nitrophenol, 97%. ×Close. 2-Chloro-4-nitrophenol, 97% Structure ... 4-(2-Pyridylazo)resorcinol monosodium salt monohydrate. ×Close. 4-(2-Pyridylazo)resorcinol monosodium salt monohydrate ...
more infohttps://www.alfa.com/en/indicators-and-dyes/
CosR is an oxidative stress sensing a MarR-type transcriptional repressor in Corynebacterium glutamicum | Biochemical Journal |...  CosR is an oxidative stress sensing a MarR-type transcriptional repressor in Corynebacterium glutamicum | Biochemical Journal |...
Novel application of 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole to identify cysteine sulfenic acid in the AhpC component of alkyl ... As shown in Figure 7, only the addition of the oxidized CosR into the Trx system showed a consumption of NADPH. No reduction in ... The formation of Cys-SOH in CosR variants is measured by the 4-chloro-7-nitrobenzofurazan (NBD-Cl) labeling assay [31-33]. ... The formation of disulfide bond and sulfenic acid in CosR WT and its variants are performed with the thiol-reactive probe 4- ...
more infohttps://portlandpress.com/biochemj/article/475/24/3979/49840/CosR-is-an-oxidative-stress-sensing-a-MarR-type
Wavelength Index | Sigma-Aldrich  Wavelength Index | Sigma-Aldrich
7-Methoxycoumarin-3-carboxylic acid N-succinimidyl ester. MeOH. 358. 410. 64958. 2-Methoxy-2,4-diphenyl-3(2H)-furanone. Amine ... 7-Hydroxy-4-methyl-3-coumarinylacetic acid. pH 10.0. 360. 445. 55627. 7-Hydroxy-4-methyl-2(1H)-quinolone. pH 5.0. 321. 357. pH ... 4-Chloro-7-nitrobenzofurazan. MeOH. 336. Aliphatic amine adducts. 475. 540. 29059. 1,3-Cyclohexanedione. Protein adduct. 375. ... 7-(Diethylamino)coumarin-3-carbonyl azide. MeOH. 432. 480. 36799. 7-(Diethylamino)coumarin-3-carboxylic acid. pH 9.0. 409. 473 ...
more infohttps://www.sigmaaldrich.com/life-science/cell-biology/detection/learning-center/wavelength-index.html
Labeling of HepG2 cells with exogenously administered C | Open-i  Labeling of HepG2 cells with exogenously administered C | Open-i
Cells were labeled with 4 μM C6NBD-GlcCer or C6-NBD-SM at 37°C for 30 min. ... Cells were labeled with 4 μM C6NBD-GlcCer or C6-NBD-SM at 37°C for 30 min. Both lipid analogues were present in the BC ( ... Cells were labeled with 4 μM C6NBD-GlcCer or C6-NBD-SM at 37°C for 30 min. Both lipid analogues were present in the BC ( ... As a control, cells were incubated in HBSS in the absence of sodiumdithionite at 4°C for 7 min. After extensively washing (15 ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC2139765_JCB.vanijzendoorn1af&req=4
SCOP 1.55: Structural Classification of Proteins - extended  SCOP 1.55: Structural Classification of Proteins - extended
Description: the structure of bovine f1-atpase covalently inhibited with 4-chloro-7-nitrobenzofurazan. Deposited on 1998-04-30 ...
more infohttp://scop.berkeley.edu/pdb/code=1nbm&ver=1.55
Elastic coupling power stroke mechanism of the F1-ATPase molecular motor | PNAS  Elastic coupling power stroke mechanism of the F1-ATPase molecular motor | PNAS
4 A and D, and of 4ASU for Fig. 4B that were simplified from SI Appendix, Fig. S1 to emphasize mechanistic features. Several ... 4). As the catalytic dwell ends (Fig. 4A), catalytic site βD hydrolyzed ATP to Pi and ADP. Behind subunit γ, catalytic site βT ... 4, ) to enable tight winding that can store elastic energy. In all F1 structures known to date, subunit γ is tethered to the ( ... 4C) is thought to result from force applied to subunit γ as a crankshaft from ATP binding-dependent closure βE-lever (i.e., the ...
more infohttps://www.pnas.org/content/115/22/5750
A Novel Liquid-Liquid Extraction for the Determination of Sertraline in Tap Water and Waste Water at Trace Levels by GC-MS,...  A Novel Liquid-Liquid Extraction for the Determination of Sertraline in Tap Water and Waste Water at Trace Levels by GC-MS,...
Quantitative analysis of sertraline in human serum by LC with fluorescence detection after pre-column derivatization with 4- ... chloro-7-nitrobenzofurazan. Bahrami, G; Mohammadi, B; Farshchi, A; Ghiasi, G. * Development of a sensitive liquid-liquid ...
more infohttps://www.deepdyve.com/lp/springer_journal/a-novel-liquid-liquid-extraction-for-the-determination-of-sertraline-HIYYbqzCCw
Find Research Outputs
             - UNT Health Science Center  Find Research Outputs - UNT Health Science Center
Prokai, L., Guo, J. & Prokai-Tatrai, K., 1 Jan 2014, In : Nature Protocols. 9, 4, p. 882-895 14 p.. Research output: ... Nguyen, V., Zharikova, A. D., Prokai-Tatrai, K. & Prokai, L., 1 Apr 2010, In : Brain Research Bulletin. 82, 1-2, p. 83-86 4 p. ... Rauniyar, N., Prokai-Tatrai, K. & Prokai, L., 1 Apr 2010, In : Journal of Mass Spectrometry. 45, 4, p. 398-410 13 p.. Research ... Prokai, L., Szarka, S., Wang, X. & Prokai-Tatrai, K., 6 Apr 2012, In : Journal of Chromatography A. 1232, p. 281-287 7 p.. ...
more infohttps://experts.unthsc.edu/en/publications/?descending=true&showAdvanced=false&allConcepts=true&inferConcepts=true&publicationYear=2010&publicationYear=2011&publicationYear=2012&publicationYear=2013&publicationYear=2014&publicationYear=2015&publicationYear=2016&publicationYear=2017&publicationYear=2018&publicationYear=2019&author=aa953fce-55b7-491d-91ec-6a59879611c8
An Overview of Analytical Determination of  Diltiazem, Cimetidine, Ranitidine, and Famotidine by  UV Spectrophotometry and HPLC...  An Overview of Analytical Determination of Diltiazem, Cimetidine, Ranitidine, and Famotidine by UV Spectrophotometry and HPLC...
Similarly, chloro drug derivative of diltiazem was formed by means of reaction involving tertiary amino group of diltiazem and ... the 8-chloro derivative of diltiazem (clentiazem maleate) and their deacetyl forms were resolved on ovomucoid-bonded chiral ... "Direct high-performance liquid chromatographic separation of the enantiomers of diltiazem hydrochloride and its 8-chloro ... Nighat Shafi,1 Farhan Ahmed Siddiqui,1 Huma Naseem,2 Nawab Sher,3 Arif Zubair,4 Azhar Hussain,5 Ali Akbar Sial,1 and Mirza ...
more infohttps://www.hindawi.com/journals/jchem/2013/184948/
Mitochondrial adenosine triphosphatase of the fission yeast Schizosaccharomyces pombe 972h-. Changes in inhibitor sensitivities...  Mitochondrial adenosine triphosphatase of the fission yeast Schizosaccharomyces pombe 972h-. Changes in inhibitor sensitivities...
4. The variations in I50 values for efrapeptin closely followed changes in specific activity of ATPase, as would be expected ... 4-chloro-7-nitrobenzofurazan, quercetin and spegazzinine, all of which show different sensitivity profiles from one another. 5 ...
more infohttp://www.biochemj.org/content/162/3/581
Terteriary Colors Stains Dyes and Indicators  Terteriary Colors Stains Dyes and Indicators
CAS: 4197-24-4 Molecular Formula: C21H22ClN3 Molecular Weight (g/mol): 351.878 MDL Number: MFCD00143923 InChI Key: ... 4-Chloro-7-nitrobenzofurazan, 4-Chloro-7-nitrobenzo-2-oxa-1, 3-diazole PubChem CID: 25043 ChEBI: CHEBI:78878 ... 7-Hydroxy-3H-phenoxazin-3-one 10-oxide, sodium salt PubChem CID: 112939 ...
more infohttps://www.fishersci.com/us/en/products/I9C8K9PL/terteriary-colors-stains-dyes-indicators.html
List of MeSH codes (D03) - Wikipedia  List of MeSH codes (D03) - Wikipedia
5-tetrahydro-8-chloro-3-methyl-5-phenyl-1h-3-benzazepin-7-ol MeSH D03.438.079.800 --- 2,3,4,5-tetrahydro-7,8-dihydroxy-1-phenyl ... 8-chloro-, 2-acetylhydrazide MeSH D03.494.347.500 --- loxapine MeSH D03.494.347.500.040 --- amoxapine MeSH D03.494.382.393 --- ... 4,5-dihydro-1-(3-(trifluoromethyl)phenyl)-1h-pyrazol-3-amine MeSH D03.383.129.539.200 --- epirizole MeSH D03.383.129.539.487 ... 4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, methyl ester MeSH D03.383.725.210 --- dimethindene MeSH D03.383. ...
more infohttps://en.wikipedia.org/wiki/List_of_MeSH_codes_(D03)
Determination of Thyreostats in Urine Using Supported Liquid Extraction and Mixed-Mode Cation-Exchange Solid-Phase Extraction:...  Determination of Thyreostats in Urine Using Supported Liquid Extraction and Mixed-Mode Cation-Exchange Solid-Phase Extraction:...
4 CRL Guidance Paper . -CRLs view on state of the art analytical methods for the national residue control plans; ( 2007 ); http ... 4 CRL Guidance Paper . -CRLs view on state of the art analytical methods for the national residue control plans; ( 2007 ); http ... Equipment and instruments Kinetex XB C-18 HPLC column (100 mm × 3 mm, 2.6 μm) and C-18 security guard column (4 mm × 2 mm); ... Equipment and instruments Kinetex XB C-18 HPLC column (100 mm × 3 mm, 2.6 μm) and C-18 security guard column (4 mm × 2 mm); ...
more infohttps://www.deepdyve.com/lp/ou_press/determination-of-thyreostats-in-urine-using-supported-liquid-4VjyGhnwQ0
NAVER 학술정보 >...  NAVER 학술정보 >...
1978 B B Kaplan 외 4 명 BIOCHEMISTRY 57회 피인용 Animals, Base Sequence, Brain Chemistry, Cell Line, Cerebellum, analysis, Cerebral ... Lateral diffusion of a hydrophobic peptide, N-4-nitrobenz-2-oxa-1,3-diazole gramicidin S, in phospholipid multibilayers.. 1978 ... start page previous page 1 2 3 4 5 next page end page ... methyl 4-mercaptobutyrimidate).. 1978 R Jue 외 3 명 BIOCHEMISTRY ... 4-Chloro-7-nitrobenzofurazan, Calorimetry, Differential Scanning, Cholesterol, Diffusion, Gramicidin, Kinetics, Membranes, ...
more infohttps://academic.naver.com/search.naver?field=3&query=BIOCHEMISTRY+17%EA%B6%8C+25%ED%98%B8
NAVER 학술정보 >...  NAVER 학술정보 >...
1993 Raditsch Martin 외 7 명 FEBS LETTERS 16회 피인용 Cloned NMDA receptor channels of the NR1-NR2A, NR1-NR2B and NR1-NR2C type show ... 1993 Saeki Tohru 외 4 명 FEBS LETTERS 28회 피인용 Understanding of the interactions between P-glycoprotein and multidrug resistance ( ... start page previous page 1 2 3 4 next page end page ... 1993 Artymiuk Peter J 외 4 명 FEBS LETTERS 12회 피인용 ... 4-Chloro-7-nitrobenzofurazan, Animals, Chickens, Dimyristoylphosphatidylcholine, Fluorescent Dyes, Gizzard, Avian, Liposomes, ...
more infohttps://academic.naver.com/search.naver?field=3&query=FEBS+LETTERS+324%EA%B6%8C+1%ED%98%B8
Probing the interaction between daptomycin and model membranes - UBC Library Open Collections  Probing the interaction between daptomycin and model membranes - UBC Library Open Collections
2004, 11 (7), 949-957. 66. Ho, S. W., et al., Effect of divalent cations on the structure of the antibiotic daptomycin. ... A serial dilution of the solution containing daptomycin in POPC/POPG/CL and 7 mM Ca2+, when the vesicles are in fused state, is ... 1986, 30 (4), 532-535. 42. Kirkpatrick, P., et al., Daptomycin. Nat. Rev. Drug Discov. 2003, 2 (12), 943-4. 43. Fowler, V. G., ... 45 Table 2.1 Kd and Kp of 4 µM daptomycin and NBD-daptomycin binding to different membrane models. Peptide Kd (µM) with DMPC/PG ...
more infohttps://open.library.ubc.ca/cIRcle/collections/ubctheses/24/items/1.0166658
INTERCHIM: Derivatization Reagents  INTERCHIM: Derivatization Reagents
INTERCHIM UPTIMA 1-FLUORO-2,4-DINITROPHENYL-5-L-ALANINE AMIDE (FDAA, MARFEY'S REAGENT) ...
more infohttp://www.interchim.com/catalogue/161/derivatization-procedures.html
  • 4-Chloro-7-nitrobenzofurazan (NBD-chloride) was used in the following studies, Synthesis of fluorescent phospholipid-derivative, NBD-didecanoylphosphatidylethanolamine, Synthesis of functionalized hydroxynaphthofurazan, Spectrophotometric and spectrofluorometric determination of clemastine hydrogen fumarate, loratadine, losartan potassium and ramipril in pharmaceutical formulations. (alfa.com)
  • It is utilized in the preparation of fluorescent phospholipid-derivative, hydroxynaphthofurazan and 4-chloro-7-nitrobenzofurazan- didecanoylphosphatidylethanolamine. (alfa.com)
  • For this, the cells were labeled with fluorescent acyl chain-labeled 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-amino]hexanoic acid (C6-NBD) derivatives of either GlcCer (C6-NBD-GlcCer) or SM (C6-NBD-SM). (nih.gov)
  • C. glutamicum cells are frequently confronted with excessive reactive oxygen species (ROS) production, triggering sudden changes of fermentative conditions in temperature, pH, osmotic pressure, or toxic compounds [ 4 - 5 ]. (portlandpress.com)
  • Cells were labeled with 4 μM C6NBD-GlcCer or C6-NBD-SM at 37°C for 30 min. (nih.gov)
  • Note that in order to visualize intracellular fluorescence in b and d, basolateral membrane-located lipid analogues were partly removed by a brief back exchange procedure (5-10 min, 4°C). Alternatively, cells incubated as in a and c were subsequently subjected to a back exchange procedure at 4°C for 30 min to completely remove the basolateral PM pool of lipid analogues. (nih.gov)
  • After back exchange, the cells were incubated in HBSS, supplemented with 30 mM sodiumdithionite at 4°C for 7 min. (nih.gov)
  • Addition of sulfhydryl groups to Escherichia coli ribosomes by protein modification with 2-iminothiolane (methyl 4-mercaptobutyrimidate). (naver.com)
  • P.B. Ghosh and M.W. Whitehouse: "7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole: a new fluorigenic reagent for amino acids and the other amines", Biochem. (edu.pl)
  • 7] K. Imai and Y. Watanabe: "Fluorimetric determination of secondary amino acids by 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole", Anal. (edu.pl)
  • L. Johnson, S. Lagerkvist, P. Lindroth, M. Ahnoff, K. Martinsson: "Derivatization of secondary amino acids with 7-nitro-4-benzofurazanyl ethers", Anal. (edu.pl)