4-Chloro-7-nitrobenzofurazan: A benzofuran derivative used as a protein reagent since the terminal N-NBD-protein conjugate possesses interesting fluorescence and spectral properties. It has also been used as a covalent inhibitor of both beef heart mitochondrial ATPase and bacterial ATPase.OxadiazolesBenzoxazolesAurovertins: Very toxic and complex pyrone derivatives from the fungus Calcarisporium arbuscula. They bind to and inhibit mitochondrial ATPase, thereby uncoupling oxidative phosphorylation. They are used as biochemical tools.Ficain: A sulfhydryl proteinase with cysteine at the active site from ficus latex. Preferential cleavage is at tyrosine and phenylalanine residues. EC 3.4.22.3.Benzofurans: Compounds that contain a BENZENE ring fused to a furan ring.Proton-Translocating ATPases: Multisubunit enzymes that reversibly synthesize ADENOSINE TRIPHOSPHATE. They are coupled to the transport of protons across a membrane.Chemistry: A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.Chemical Phenomena: The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.Mitochondria, Heart: The mitochondria of the myocardium.2-Chloroadenosine: 2-Chloroadenosine. A metabolically stable analog of adenosine which acts as an adenosine receptor agonist. The compound has a potent effect on the peripheral and central nervous system.Halogens: A family of nonmetallic, generally electronegative, elements that form group 17 (formerly group VIIa) of the periodic table.Sulfhydryl Compounds: Compounds containing the -SH radical.ChlorobenzenesBenztropine: A centrally active muscarinic antagonist that has been used in the symptomatic treatment of PARKINSON DISEASE. Benztropine also inhibits the uptake of dopamine.Kinetics: The rate dynamics in chemical or physical systems.Lysine: An essential amino acid. It is often added to animal feed.Structure-Activity Relationship: The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Chlorine: A greenish-yellow, diatomic gas that is a member of the halogen family of elements. It has the atomic symbol Cl, atomic number 17, and atomic weight 70.906. It is a powerful irritant that can cause fatal pulmonary edema. Chlorine is used in manufacturing, as a reagent in synthetic chemistry, for water purification, and in the production of chlorinated lime, which is used in fabric bleaching.Catechols: A group of 1,2-benzenediols that contain the general formula R-C6H5O2.Biodegradation, Environmental: Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.Stereoisomerism: The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Molecular Structure: The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.Magnetic Resonance Spectroscopy: Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Corynebacterium glutamicum: A species of gram-positive, asporogenous, non-pathogenic, soil bacteria that produces GLUTAMIC ACID.Corynebacterium: A genus of asporogenous bacteria that is widely distributed in nature. Its organisms appear as straight to slightly curved rods and are known to be human and animal parasites and pathogens.Glutathione Peroxidase: An enzyme catalyzing the oxidation of 2 moles of glutathione in the presence of hydrogen peroxide to yield oxidized glutathione and water. EC 1.11.1.9.Lac Operon: The genetic unit consisting of three structural genes, an operator and a regulatory gene. The regulatory gene controls the synthesis of the three structural genes: BETA-GALACTOSIDASE and beta-galactoside permease (involved with the metabolism of lactose), and beta-thiogalactoside acetyltransferase.Monkeypox virus: A species of ORTHOPOXVIRUS causing an epidemic disease among captive primates.beta-Galactosidase: A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.Monkeypox: A viral disease infecting PRIMATES and RODENTS. Its clinical presentation in humans is similar to SMALLPOX including FEVER; HEADACHE; COUGH; and a painful RASH. It is caused by MONKEYPOX VIRUS and is usually transmitted to humans through BITES or via contact with an animal's BLOOD. Interhuman transmission is relatively low (significantly less than smallpox).Famotidine: A competitive histamine H2-receptor antagonist. Its main pharmacodynamic effect is the inhibition of gastric secretion.Ranitidine: A non-imidazole blocker of those histamine receptors that mediate gastric secretion (H2 receptors). It is used to treat gastrointestinal ulcers.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Cimetidine: A histamine congener, it competitively inhibits HISTAMINE binding to HISTAMINE H2 RECEPTORS. Cimetidine has a range of pharmacological actions. It inhibits GASTRIC ACID secretion, as well as PEPSIN and GASTRIN output.Spectrophotometry, Ultraviolet: Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Access to Information: Individual's rights to obtain and use information collected or generated by others.Diltiazem: A benzothiazepine derivative with vasodilating action due to its antagonism of the actions of CALCIUM ion on membrane functions.Schizosaccharomyces: A genus of ascomycetous fungi of the family Schizosaccharomycetaceae, order Schizosaccharomycetales.Trialkyltin Compounds: Organometallic compounds which contain tin and three alkyl groups.Schizosaccharomyces pombe Proteins: Proteins obtained from the species Schizosaccharomyces pombe. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.Adenosine Triphosphatases: A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.Oxidative Phosphorylation Coupling FactorsCarbodiimidesBrain Chemistry: Changes in the amounts of various chemicals (neurotransmitters, receptors, enzymes, and other metabolites) specific to the area of the central nervous system contained within the head. These are monitored over time, during sensory stimulation, or under different disease states.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Cerebral Cortex: The thin layer of GRAY MATTER on the surface of the CEREBRAL HEMISPHERES that develops from the TELENCEPHALON and folds into gyri and sulchi. It reaches its highest development in humans and is responsible for intellectual faculties and higher mental functions.Cerebellum: The part of brain that lies behind the BRAIN STEM in the posterior base of skull (CRANIAL FOSSA, POSTERIOR). It is also known as the "little brain" with convolutions similar to those of CEREBRAL CORTEX, inner white matter, and deep cerebellar nuclei. Its function is to coordinate voluntary movements, maintain balance, and learn motor skills.Hypothalamus: Ventral part of the DIENCEPHALON extending from the region of the OPTIC CHIASM to the caudal border of the MAMMILLARY BODIES and forming the inferior and lateral walls of the THIRD VENTRICLE.Hippocampus: A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.

Specific and sensitive assay for alkaline and neutral ceramidases involving C12-NBD-ceramide. (1/347)

A fluorescent analogue of ceramide, C12-NBD-ceramide, was found to be hydrolyzed much faster than 14C-labeled ceramide by alkaline ceramidase from Pseudomonas aeruginosa and neutral ceramidase from mouse liver, while this substrate was relatively resistant to acid ceramidase from plasma of the horseshoe crab. The radioactive substrate was used more preferentially by the acid ceramidase. It should be noted that C6-NBD-ceramide, which is usually used for ceramidase assays, was hardly hydrolyzed by any of the enzymes examined, compared to C12-NBD-ceramide. For the alkaline and neutral enzymes, the Vmax and k (Vmax/Km) with C12-NBD-ceramide were much higher than those with 14C-ceramide. In contrast, for the acid enzyme these parameters with C12-NBD-ceramide were less than half those with the radioisotope-labeled substrate. It is noteworthy that the labeling of ceramide with NBD did not itself reduce the Km of the alkaline enzyme, but did that of the neutral enzyme. It was also found that C12-NBD-ceramide was preferentially hydrolyzed by the alkaline and neutral enzymes, but not the acid one, in several mammalian cell lines. This study clearly shows that the attachment of NBD, but not dansyl, increases the susceptibility of ceramide to alkaline and neutral enzyme, and decreases that to acid enzymes. Thus the use of this substrate provides a specific and sensitive assay for alkaline and neutral ceramidases.  (+info)

Maturation of the axonal plasma membrane requires upregulation of sphingomyelin synthesis and formation of protein-lipid complexes. (2/347)

Neuronal maturation is a gradual process; first axons and dendrites are established as distinct morphological entities; next the different intracellular organization of these processes occurs; and finally the specialized plasma membrane domains of these two compartments are formed. Only when this has been accomplished does proper neuronal function take place. In this work we present evidence that the correct distribution of a class of axonal membrane proteins requires a mechanism which involves formation of protein-lipid (sphingomyelin/cholesterol) detergent-insoluble complexes (DIGs). Using biochemistry and immunofluorescence microscopy we now show that in developing neurons the randomly distributed Thy-1 does not interact with lipids into DIGs (in fully developed neurons the formation of such complexes is essential for the correct axonal targeting of this protein). Using lipid mass spectrometry and thin layer chromatography we show that the DIG lipid missing in the developing neurons is sphingomyelin, but not cholesterol or glucosylceramide. Finally, by increasing the intracellular levels of sphingomyelin in the young neurons the formation of Thy-1/DIGs was induced and, consistent with a role in sorting, proper axonal distribution was facilitated. These results emphasize the role of sphingomyelin in axonal, and therefore, neuronal maturation.  (+info)

Saturable stimulation of fatty acid transport through model cytoplasm by soluble binding protein. (3/347)

To better define the role of soluble binding proteins in the cytoplasmic transport of amphipathic molecules, we measured the diffusional mobility of a fluorescent long-chain fatty acid, 12-N-methyl-(7-nitrobenz-2-oxa-1,3-diazol)aminostearate (NBD-stearate), through model cytoplasm as a function of soluble binding protein concentration. Diffusional mobilities were correlated with the partition of the fatty acid between membrane and protein binding sites. Cytoplasm was modeled as a dense suspension of liposomes, and albumin was used as a model binding protein. Albumin saturably increased NBD-stearate mobility through the membrane suspension approximately eightfold. Fatty acid mobility in the absence of albumin was identical to the mobility of the membrane vesicles (1.99 +/- 0.33 x 10(-8) cm(2)/s), whereas the mobility at saturating concentrations was identical to the mobility of albumin (1.65 +/- 0.12 x 10(-7) cm(2)/s). The protein concentration producing half-maximal stimulation of NBD-stearate diffusion (42.8 +/- 0.3 microM) was unexpectedly greater than that required to solubilize half of the NBD-stearate (17.9 +/- 3.0 microM). These results support a proposed mechanism for cytoplasmic transport of small amphipathic molecules in which aqueous diffusion of the protein-bound form of the molecule largely determines the transport rate. However, slow interchange of fatty acid between the binding protein and membranes also appears to influence the transport rate in this model system.  (+info)

Fluorescent phosphoinositide derivatives reveal specific binding of gelsolin and other actin regulatory proteins to mixed lipid bilayers. (4/347)

Fluorescent derivatives of phosphatidyl inositol (PtdIns)-(4,5)-P2 were synthesized and used to test the effects of the PtdIns-(4, 5)-P2-regulated proteins gelsolin, tau, cofilin, and profilin on labeled PtdIns-(4,5)-P2 that was either in micellar form or mixed with phosphatidylcholine (PtdCho) in bilayer vesicles. Gelsolin increased the fluorescence of 7-nitrobenz-2-oxa-1,3-diazole (NBD)- or pyrene-labeled PtdIns-(4,5)-P2 and NBD-PtdIns-(3,4,5)-P3. Cofilin and profilin produced no detectable change at equimolar ratios to PtdIns-(4,5)-P2, while tau decreased NBD-PtdIns-(4,5)-P2 fluorescence. Fluorescence enhancement by gelsolin of NBD-PtdIns-(4, 5)-P2 in mixed lipid vesicles depended on the mole fraction of PtdIns-(4,5)-P2 in the bilayer. Specific enhancement of 3% NBD-PtdIns-(4,5)-P2 : 97% PtdCho was much lower than that of 10% PtdIns-(4,5)-P2 : 90% PtdCho, but the enhancement of 3% NBD-PtdIns-(4,5)-P2 could be increased by addition of 7% unlabeled PtdIns-(4,5)-P2. The gelsolin-dependent increase in NBD-PtdIns-(4, 5)-P2 fluorescence was reversed by addition of Ca2+ or G-actin. Significant, but weaker, fluorescence enhancement was observed with the gelsolin N-terminal domain (residues 1-160) and a peptide comprised of gelsolin residues 150-169. Fluorescence energy transfer from gelsolin to pyrene-PtdIns-(4,5)-P2 was much stronger with intact gelsolin than the N-terminal region of gelsolin containing the PtdIns-(4,5)-P2 binding sites, suggesting that PtdIns-(4,5)-P2 may bind near a site formed by the juxtaposition of the N- and C-terminal domains of gelsolin.  (+info)

Rapid transbilayer movement of fluorescent phospholipid analogues in the plasma membrane of endocytosis-deficient yeast cells does not require the Drs2 protein. (5/347)

Evidence is presented that endocytosis-deficient Saccharomyces cerevisiae end4 yeast cells rapidly internalize the fluorescent phospholipid analogues 1-palmitoyl-2-{6-[7-nitro-2,1, 3-benzoxadiazol-4-yl(NBD)amino] caproyl}phosphatidylcholine (P-C6-NBD-PtdCho) and P-C6-NBD-phosphatidylserine (P-C6-NBD-PtdSer). Both analogues redistributed between the exoplasmic and cytoplasmic leaflet with a half-time of < 15 min at 0 degrees C. The plateau of internalized analogues was about 70%. Transbilayer movement is probably protein-mediated, as the flip-flop of both analogues was very slow in liposomes composed of plasma-membrane lipids. Rapid analogue internalization was not abolished on depletion of intracellular ATP by about 90%. For P-C6-NBD-PtdCho only was a moderate decrease in the plateau of internalized analogues of about 20% observed, while that of P-C6-NBD-PtdSer was not affected. The Drs2 protein plays only a minor role, if any, in the rapid transbilayer movement of analogues in S. cerevisiae end4 cells. In S. cerevisiae end4 Deltadrs2 cells harbouring both an end4 allele and a drs2 null allele, about 60% and 50% of P-C6-NBD-PtdCho and P-C6-NBD-PtdSer, respectively, became internalized within 15 min at 0 degrees C. The preferential orientation of P-C6-NBD-PtdSer to the cytoplasmic leaflet is in qualitative agreement with the sequestering of endogenous phosphatidylserine to the cytoplasmic leaflet, as assessed by binding of annexin V. Virtually no binding of annexin V to spheroplasts of the parent wild-type strain or the mutant strains was observed. Likewise, no difference in the exposure of endogenous aminophospholipids to the exoplasmic leaflet between these strains was found by labelling with trinitrobenzenesulfonic acid. Thus, lipid asymmetry, at least of aminophospholipids, was preserved in S. cerevisiae end4 cells independently of the presence of the Drs2 protein.  (+info)

Cytoplasmic transport of fatty acids in rat enterocytes: role of binding to fatty acid-binding protein. (6/347)

The intracellular movement of fatty acids is thought to be facilitated through codiffusion with fatty acid-binding protein (FABP). This facilitation may occur by decreasing binding to immobile membranes, leading to faster cytoplasmic diffusion. The aims of this study were to measure the intracellular transport of 12-N-methyl-(7-nitrobenzo-2-oxa-1,3-diazol)aminostearate (NBD-stearate) in villus rat enterocytes and to determine 1) the mechanism of its cytoplasmic transport and 2) if its transport rate correlated with the known variation of FABP binding capacity along the length of the small intestine. Two-dimensional laser photobleaching was used to measure the movement of a fluorescent fatty acid NBD-stearate in enterocytes isolated from different segments of rat intestine. The fraction of NBD-stearate found in the cytostol of enterocytes was determined by differential centrifugation. Cytoplasmic transport of NBD-stearate occurred solely by diffusion and not by convection. Diffusion was homogeneous (nondirectional), consistent with isotropic diffusion. The diffusion rate varied with location along the intestine, correlating with the local FABP concentration and measured cytosolic binding. We conclude that cytoplasmic proteins like FABP promote the intracellular transport of fatty acids by enhancing their diffusive flux. We suggest that facilitation is not specific for a particular cell type but occurs in a variety of cells that transport fatty acids and may contain different types of FABP.  (+info)

Brownian ratchets: molecular separations in lipid bilayers supported on patterned arrays. (7/347)

Brownian ratchets use a time-varying asymmetric potential that can be applied to separate diffusing particles or molecules. A new type of Brownian ratchet, a geometrical Brownian ratchet, has been realized. Charged, fluorescently labeled phospholipids in a two-dimensional fluid bilayer were driven in one direction by an electric field through a two-dimensional periodic array of asymmetric barriers to lateral diffusion fabricated from titanium oxide on silica. Diffusion spreads the phospholipid molecules in the orthogonal direction, and the asymmetric barriers rectify the Brownian motion, causing a directional transport of molecules. The geometrical ratchet can be used as a continuous molecular sieve to separate mixtures of membrane-associated molecules that differ in electrophoretic mobility and diffusion coefficient.  (+info)

Location of the catalytic nucleophile of phospholipase D of Streptomyces antibioticus in the C-terminal half domain. (8/347)

Phospholipase D (PLD) of Streptomyces antibioticus was labelled with fluorescent-labelled substrate, 1-hexanoyl-2-{6-[(7-nitro-2-1, 3-benzoxadiazol-4-yl)-amino]hexanoyl}-sn-glycero-3-phosphocholine, when it was incubated with the substrate and the reaction followed by SDS/PAGE. Mutant enzymes lacking the catalytic activity were not labelled under the same conditions, indicating that labelling of the PLD occurred as the result of its catalytic action. This confirmed that the labelled protein was the phosphatidyl PLD intermediate. PLDs contain two copies of the highly conserved catalytic HxKxxxxD (HKD) motif. Therefore, two protein fragments were separately prepared with recombinant strains of Escherichia coli. One of the fragments was the N-terminal half of the intact PLD containing one HKD motif, and the other was the C-terminal half with the other motif. An active enzyme was reconstructed from these two fragments, and therefore designated fragmentary PLD (fPLD). When fPLD was subjected to the labelling experiment, only the C-terminal half was labelled. Therefore, it was concluded that the catalytic nucleophile that bound directly to the phosphatidyl group of the substrate was located on the C-terminal half of PLD, and that the N-terminal half did not contain such a nucleophile.  (+info)

Learn more about 4-chloro-7-nitrobenzofurazan. We enable science by offering product choice, services, process excellence and our people make it happen.
Using brush-border membrane vesicles isolated from calf kidney cortex the effect of tyrosine-reactive reagents on sodium-dependent D-glucose transport was investigated. Treatment of the membranes for 60 min with NBD-Cl (7-chloro-4-nitrobenzo-2-oxa-1,3-diazole), N-acetylimidazole or tetranitromethane decreased D-glucose uptake 50, 70 and 40%, respectively. Tracer exchange experiments revealed that the inhibition of transport is due to a direct modification of the sodium-D-glucose cotransport system. The modification by NBD-Cl decreases the apparent Vmax of the transport system with respect to its interaction with sodium. In addition, the rate of inactivation of the transport system by NBD-Cl is reduced in the presence of high concentrations of sodium. The results indicate that tyrosine residues play an essential role in sodium-D-glucose cotransport and are probably involved in the binding and/or transport of sodium by the sodium-D-glucose cotransport system.
D-Glucose is one of the most important energy sources for the survival of various organisms, from E. colito mammals. For live-cell monitoring of glucose uptake at the single-cell level, a fl uorescent D-glucose derivative 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4- yl)amino]-2-deoxy-D-glucose [2-NBDG], which we developed, has been widely used invarious research fi elds. For the last ten years, however, researchers have awaited an optical control substance forevaluating the extent of non-specifi c adsorption of 2-NBDG upon plasma membrane and/or the rate of unhealthy2-NBDG uptake through partially( or transiently) damaged membrane. Here we introduce a fluorescent L-glucose derivative, 2-[N(- 7-Nitrobenz-2-oxa- 1,3-diazol-4-yl)amino]- 2-deoxy-Lglucose[2-NBDLG]. L-Glucosamine is a key intermediate toward the synthesis of 2-NBDLG, but not commerciallyavailable. Although a few papers on the synthesis of L-glucosamine have been reported, a new synthetic method ofL-glucosamine should be absolutely
I am planning to label some amino acids with NBD-F (4-fluoro-7-nitrobenzofurazan). In some papers Ive read, these derivatives are stated to be very stable. In other papers, they are said to be stable for up to two days when stored in the dark in a refrigerator. Does anyone know what the best storage conditions are? Although this is just a guess, I would think that evaporating the samples to dryness under nitrogen (or with a SpeedVac), and then storing them in a freezer, would increase their storage life. Of course, this assumes that they are more stable when dry, which may not be the case. Anyone know what conditions are most favorable for the preservation/storage of NBD-labeled amino acids? Phil Calvert . -- ------------------------------------ !-----------Phil Calvert-----------! !---calvert.NoSpam at eos.ncsu.edu----! ------------------------------------ NOTE: If present, the phrase .NoSpam must be removed from my return address before sending your reply ...
This study was undertaken to determine whether 4-benzylamino-7-nitrobenz-2-oxa-l,3-diazole (BBD) generates the first excited singlet state of molecular oxygen in liquid solution. The problem was first approached using the ...
Learn more about 1-3-chloro-4-methoxyphenyl-sulfonyl-piperazine. We enable science by offering product choice, services, process excellence and our people make it happen.
Fluorescent glucose biosensors are devices that measure the concentration of glucose in diabetic patients by means of sensitive protein that relays the concentration by means of fluorescence, an alternative to amperometric sension of glucose. No device has yet entered the medical market, but, due to the prevalence of diabetes, it is the prime drive in the construction of fluorescent biosensors. Keeping glucose levels in check is crucial to minimize the onset of the damage caused by diabetes. As a consequence, in conjunction with insulin administrations, the prime requirement for diabetic patients is to regularly monitor their blood glucose levels. The monitoring systems currently in general use have the drawback of below optimal number of readings, due to their reliance on a drop of fresh blood. Some continuous glucose monitors are commercially available, but suffer from the severe drawback of a short working life of the probe. The majority of these work amperometrically. As a result, there is ...
Changes in the spectral properties of plasma membrane lipid analog during the first seconds of endocytosis in living cells, Biophys. , 72, 32-50, 1997. , A novel fluorescent ceramide analog for studying membrane traffic in animal cells; accumulation at the Golgi apparatus results in altered spectral properties of the sphingoid precursor, J. , 113, 1267-1279, 1991. 7 mM), a fluorescent probe of the Golgi apparatus. A leopard-skin appearance of fluorochrome-loaded Golgi sacks is obtained. Similar fluorescence images may be recorded when the cell is treated with fluorescent cytotoxic agents (see Chapter 3). Doctoral dissertation, Carnegie-Mellon University, Pittsburgh, PA, 1990. fm Page 14 Tuesday, November 18, 2003 8:40 AM 14 Atlas of Cell Fluorescence FIGURE 12 Lysosomes with lysososomotropic agent quinacrine. Color image; microphotograph. fm Page 15 Tuesday, November 18, 2003 8:40 AM Vital Fluorescence Probes of Cell Organelles FIGURE 13 Quinacrine imaging of lysosomes. SP micrograph. fm Page 16 ...
The sensor was successfully deployed in all five patients and did not interfere with clinical care, blood pressure monitoring or sampling. One patient suffered a cardiopulmonary arrest; the sensor functioned successfully during resuscitation and urgent return to the operating room. One hundred and twenty reference samples ranging from 5.9 to 13.4 mmol/l were collected; 107/120 (89.2%) of GluCath measurements met ISO 15197 criteria (within ±20% of reference when BG ,4.2 mmol/l; Figure 1). In Subject 1 the sensor was inadvertently retracted into the arterial catheter during the study, leading to measurement error from arterial flush solution contamination. In a sensitivity analysis excluding this patient, 89/95 (93.7%) of measurements met ISO 15197 with a mean absolute relative difference of 9.4 ...
Boc Sciences offers cas 864685-37-0 Fmoc-{[4-(6-t-butoxycarbonyl)hexanoyl]piperazin-4-yl}-glycine in bulk,please inquire us to get a quote for 864685-37-0 Fmoc-{[4-(6-t-butoxycarbonyl)hexanoyl]piperazin-4-yl}-glycine.
This work has been made available to the staff and students of the University of Sydney for the purposes of research and study only. It constitutes material that is held by the University for the purposes of reporting for HERDC and the ERA. This work may not be downloaded, copied and distributed to any third party ...
We,China 4-chloro-6-iodoquinazoline 98556-31-1 Suppliers and China 4-chloro-6-iodoquinazoline 98556-31-1 Manufacturers, provide 4-chloro-6-iodoquinazoline 98556-31-1 product and the products related with China 4-chloro-6-iodoquinazoline 98556-31-1 - princechem
Item #: SCP-1816. Object Class: Safe. Special Containment Procedures: SCP-1816 is confined in an underground experimental room measuring 10m x 10m x 5m. The room is climate-controlled and equipped with sunlight-simulating lamps. Access to SCP-1816 is forbidden to all female personnel, unless approved by Dr. Coulloudon. The maintenance of SCP-1816, including pruning, fertilization and re-potting, is to be conducted by Professor H. Pak.. Description: SCP-1816 is a penjing containing a live specimen of an unidentified tree. The object is approximately 40 cm high.. SCP-1816 will only affect pregnant mammals. When a subject at an early stage of pregnancy is left in the same room as SCP-1816, the fetus may become an instance of SCP-1816-1. The distance and time of exposure required to cause this effect depends on the size and gestation period of the individual. In the case of murine test subjects, an exposure of 3 minutes/day within 5 meters was found to be sufficient. The requirements for larger ...
Begin Log,. Dr. Hayfield: How long have you been connected to SCP-1884-B?. SCP-1884-A: As long as I can remember. Wherever I have been, Luana has been there with me, even if only in my mind.. Dr. Hayfield: Where did SCP-1884-B come from?. SCP-1884-A: When I was still very young, I asked my mother the same question. She would not tell me. She said she did not want to frighten me.. Dr. Hayfield: Have your blindness and physical abnormalities been present since birth?. SCP-1884-A: Yes. Luana has always been my eyes. She feels the ground so I can walk. She helps me hold things. In my old age, there have been times she has carried me. I am very grateful to her.. Dr. Hayfield: What were the events that led to the incident at the hotel?. SCP-1884-A: That may take some time to explain.. Dr. Hayfield: Thats perfectly fine. Please proceed.. SCP-1884-A: When I was eight years old, men came to our house asking to buy me and Luana. My parents were upset. They always tried to hide us and keep us a secret, ...
We,China 4-(3-chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-ol 184475-71-6 Suppliers and China 4-(3-chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-ol 184475-71-6 Manufacturers, provide 4-(3-chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-ol 184475-71-6 product and the products related with China 4-(3-chloro-4-fluorophenylamino)-7-methoxyquinazolin-6-ol 184475-71-6 - princechem
5-chloro-2-methyl-4-nitroaniline chemical properties, What are the chemical properties of 5-chloro-2-methyl-4-nitroaniline 13852-51-2, What are the physical properties of 5-chloro-2-methyl-4-nitroaniline ect.
Guidchem offer qualified suppliers for 9H-Purine,6-chloro-2-fluoro- (CAS NO.1651-29-2) ,Find latest products of 9H-Purine,6-chloro-2-fluoro- manufacturers, suppliers, exporters and producers on guidechem.com.
Experimentation log for SCP-682-2 Item: SCP-053 Termination Test Record: When the subject was first introduced into SCP-053s containment cell, its reaction cou
Boc Sciences offers cas 64358-13-0 3-Chloro-4-n-propylbenzophenone in bulk,please inquire us to get a quote for 64358-13-0 3-Chloro-4-n-propylbenzophenone.
Alfa Aesar is a leading manufacturer and supplier of research chemicals, pure metals and materials for a wide span of applications.
Alfa Chemistry is the worlds leading provider for special chemicals. We offer qualified products for 51522-07-7(4-chloro-3-methylsulfonyl-benzoic acid),please inquire us for 51522-07-7(4-chloro-3-methylsulfonyl-benzoic acid).
China 6-Chloro-1-Hydroxibenzotriazol (Cl-HOBt) [26198-19-6], Find details about China Cl-Hobt, 26198-19-6 from 6-Chloro-1-Hydroxibenzotriazol (Cl-HOBt) [26198-19-6] - Shanghai Medpep Co., Ltd.
ethyl 7-chloro-4-hydroxy-3-quinolinecarboxylate - chemical structural formula, chemical names, chemical properties, synthesis references
We have examined intracellular transport and metabolism of the fluorescent analogue of phosphatidylserine, 1-palmitoyl-2-(N-[12[(7-nitrobenz-2-oxa-1,3-diazole-4-yl)amino] dodecanoyl])-phosphatidylserine ([palmitoyl-C12-NBD]-PS) in cultured fibroblasts. When monolayer cultures were incubated with liposomes containing (palmitoyl-C12-NBD)-PS at 37 degrees C, fluorescent PS was transported to the Golgi apparatus. NBD-containing analogues of phosphatidylcholine, phosphatidylethanolamine (PE), or phosphatidic acid did not accumulate in the Golgi apparatus under the same experimental conditions. We suggest that the transport is not due to endocytosis, but is the result of incorporation and trans-bilayer movement of the (palmitoyl-C12-NBD)-PS at the plasma membrane followed by translocation of the lipid from plasma membrane to the Golgi apparatus via nonvesicular mechanisms. Uptake of fluorescent PS was inhibited by depletion of cellular ATP and was blocked by structural analogues of the lipid or by ...
Materials and cell culture. 1,2-Dioleoyl-sn-glycero-3-phosphocholine, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, N-hexanoyl-d-erythro-sphingosine (C6), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy polyethylene glycol-2000], N-octanoyl-sphingosine-1-[succinyl(methoxy polyethylene glycol-750)] (PEG(750)-C8), N-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-d-erythro-sphingosine (NBD-C6), and N,N-dimethyl-d-erythro-sphingosine (DMSph) were purchased from Avanti Polar Lipids (Alabaster, AL). N-hexanoyl-d-erythro-sphingosine [hexanoyl 6-3H] ([3H]C6) was obtained from ARC (St. Louis, MO). Primary antibodies to caveolin-1 and CD31 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA) and BD PharMingen (San Jose, CA), respectively. Horseradish peroxidase-conjugated goat anti-rabbit IgG and rhodamine red-X-conjugated goat anti-rat IgG secondary antibodies were obtained from Santa Cruz Biotechnology and Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA), ...
You are viewing an interactive 3D depiction of the molecule 2-({6-[(bromoacetyl)amino]hexanoyl}amino)-2-deoxy-d-glucose (C14H25BrN2O7) from the PQR.
The time course of interaction of caldesmon with actin may be monitored by fluorescence changes that occur upon the binding of 12-(N-methyl-N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl))-labeled caldesmon to actin or to acrylodan ...
Sigma-Aldrich offers abstracts and full-text articles by [Raiyan T Zaman, Hisanori Kosuge, Guillem Pratx, Colin Carpenter, Lei Xing, Michael V McConnell].
PeproTech the producer of high quality recombinant cytokines and growth factors supports life science research, cellular therapy and regenerative medicine.
Name: 2-Chloro-4-nitrobenzenamine CA Name: Benzenamine,2-chloro-4-nitro- Molecular Structure: 2-Chloro-4-nitrobenzenamine,Benzenamine,2-chloro-4-nitro-,CAS 121-87-9,172.57,C6H5ClN2O2 2-Chloro-4-nitrobenzenamine,Benzenamine,2-chloro-4-nitro-,CAS 121-87-9,172.57,C6H5ClN2O2 Molecular Formula:C6H5ClN2O2 Molecular Weight: 172.57 CAS Registry Number: 121-87-9
Find quality suppliers and manufacturers of 4999-45-5(1H-2,1,3-Benzothiadiazin-4(3H)-one, 6-chloro-3-ethyl-1-methyl-, 2-oxide) for price inquiry. where to buy 4999-45-5(1H-2,1,3-Benzothiadiazin-4(3H)-one, 6-chloro-3-ethyl-1-methyl-, 2-oxide).Also offer free database of 4999-45-5(1H-2,1,3-Benzothiadiazin-4(3H)-one, 6-chloro-3-ethyl-1-methyl-, 2-oxide) including MSDS sheet(poisoning, toxicity, hazards and safety),chemical properties,Formula, density and structure, solution etc.
Sodium 5-chloro-2-(4-chloro-2-nitrophenoxy)benzenesulfonate/ACM85136034 can be provided in Alfa Chemistry. We are dedicated to provide our customers the best products and services.
Alfa Aesar™ 2-Chloro-6-fluorophenylacetonitrile, 98% 25g Alfa Aesar™ 2-Chloro-6-fluorophenylacetonitrile, 98% Chlorot to Chola -Organics
Alfa Aesar™ N-n-Butyl-4-chloro-3-sulfamoylbenzamide, 95% 250mg Alfa Aesar™ N-n-Butyl-4-chloro-3-sulfamoylbenzamide, 95% Nonaf to Nz -Organics
2-CHLORO-6-METHOXY-3-QUINOLINECARBALDEHYDEOXIME Cas No 93299-50-4 - A wide of 2-CHLORO-6-METHOXY-3-QUINOLINECARBALDEHYDEOXIME knowledge covers characteristics, safety, usage, MSDS/SDS & Reach registration data at Echemi.com
7-chloro-2-ethyl-4-oxo-3,4-dihydro-6-quinazolinesulfonamide - chemical structural formula, chemical names, chemical properties, synthesis references
Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.. ...
Era of polyurethane foam cells, an necessary stage for change cholesterol transportation research, uses the technique of receptor-dependent macrophage launching with radiolabeled acetylated LDL. of lyso-PtdCho Cholesterol was solubilized in PBS by mixing lyso-PtdCho and cholesterol jointly. To determine if lyso-PtdCho could boost the solubility of cholesterol in PBS, continuous quantities of cholesterol, NBD-cholesterol, and an raising quantity of lyso-PtdCho had been used in five cup pipes. After drying out, aqueous solution was produced with 1 ml PBS as defined in Methods and Textiles. Ready blended micelles included 1 millimeter cholesterol (200 d from 5 millimeter share) and 5 Meters NBD-cholesterol (2.5 l from 2 mM stock) and lyso-PtdCho varying from 0C500 M (0, 2.5, 5, 12.5, and 25 d from 20 mM share). Fluorescence strength was motivated in the very clear filtrate. Fluorescence strength of the aqueous option containing only cholesterol and fluorescent cholesterol (but no lyso-PtdCho) was ...
When bovine heart mitochondrial F1-ATPase was labeled with low concentrations of NBD-Cl* in the dark in the presence of ATP at pH 7, the NBD-label was almost exclusively attached to a specific...
The sheath of ,I,Leptothrix cholodnii,/I, is a glycoconjugate composed of a polysaccharide and a peptide rich in cysteine. In this study, structural determination of the hydrazinolyzate of the sheath was carried out. Since the hydrazinolyzate is a polysaccharide incorporated with cysteine, it was ,I,S,/I,-derivatized with a thiol-specific fluorogenic reagent, 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (ABD-F). Fluorescent fragments were purified by HPLC, and their structures were analyzed by mass spectrometry and NMR spectroscopy. The sheath was found to contain 2-(cysteinyl)amido-2-deoxy-,small,D,/small,-galacturonic acid residue.. ...
2440 We previously reported that EGCG inhibits growth and activation of the EGF receptor (EGFR) and downstream signaling pathways in human colon cancer cells (Shimizu et al., Clin. Cancer Res. 11, 2735-2746, 2005), but the precise mechanism is not known. Several proteins, including EGFR, are believed to partition into detergent-insoluble liquid ordered membrane domains enriched in cholesterol, sphingomyelin, and gangliosides, so-called "lipid rafts". Therefore, we examined the effects of EGCG on ordered membrane domains in HT29 colon cancer cells. When the cells were first treated with the fluorescent lipid analog DiIC16,, which preferentially incorporates into ordered domains in the plasma membrane, subsequent treatment with EGCG caused a marked increase the sensitivity of the plasma membrane to extraction by cold Triton X-100, indicating that liquid ordered domains are decreased. Pretreatment with EGCG also inhibited subsequent incorporation of DiIC16 into the plasma membrane, indicating that ...
We report a new 7-nitrobenzo-2-oxa-1, 3-diazolyl (NBD)-based chemosensor containing a piperazine derivative, NBDP, for detection of mercury ions in almost 100% aqueous medium. The chemosensor shows sensing exclusively toward Hg2+ with a switch-on fluorescence response at 543 nm, which could be attributed to the blocking of PET (photo-induced electron transfer) process upon complexation with mercur ...
Item #: SCP-3068. Object Class: Euclid Keter. Special Containment Procedures: Several long-range Scranton Reality Cannons are trained on SCP-3068. These are to fire upon any containment breaches by SCP-3068-A instances, preventing these instances from existence in ordinary space without the need for costly physical assaults.. It should be noted that Scranton Reality Cannons are an unstable prototype. Their usage is to be restricted to SCP-3068 only. Extreme caution must be observed in their operation.. A diplomatic presence is to be maintained on SCP-3068 at all times. Travel to and from SCP-3068 is to take place using vessels fitted with a Scranton Reality Engine; again, this is an unstable prototype which should only be operated with extreme caution.. Update 09/07/2016: Attempts to locate SCP-3068-B are currently underway. Current protocol is to take SCP-3068-B in for questioning, with the intention of ascertaining its exact link to SCP-3068.. Update 05/10/2017: All use of Scranton Reality ...
Item #: SCP-086. Object Class: Keter. Special Containment Procedures: Item SCP-086 is stored in a heavily soundproofed room, isolated from the rest of the facility by an airlock.. As there is some leakage from SCP-086, the surrounding area is also cordoned off. Object is currently safe, provided no unprotected personnel enter the containment zone, so handling instructions are only provided for the possibility of a security breach, or if needing to move SCP-086.. Unprotected personnel who enter the containment zone should be removed from the area as soon as possible. If they return, or show signs of being compromised by SCP-086, they must be terminated; warnings of these consequences must be displayed prominently around the containment zone.. Description: SCP-086 appears to be a regular tetrahedron approximately 25cm on each side. It constantly emits sounds described as whispering by survivors of its effects, though in no known language.. These sounds are capable of a wide range of effects on ...
Alfa Aesar™ 4-Chloro-2-fluoro-3-methoxybenzoyl chloride, 97% 1g Alfa Aesar™ 4-Chloro-2-fluoro-3-methoxybenzoyl chloride, 97% Chlorofluorobenzi to...
Item #: SCP-3083. Object Class: Euclid. Special Containment Procedures: The orbit of the SCPS Kama has been altered to allow for easier monitoring of SCP-3083. The status of SCP-3083 is to be checked once daily, and its orientation noted if an activation event has occurred. SCP-3083-1 should always be retrieved if present, however samples of SCP-3083 need only be taken if its form is visibly altered. SCP-3083-1 instances are to be transferred to Site-22 during routine cargo exchanges with the Kama and stored in a secure locker.. As SCP-3083 is too small to be observed by amateur telescopes, disinformation campaigns need only be directed at major observatories. As the number of independent sightings since containment has been miniscule, disinformation is considered a Foxtrot-Omega Priority containment task.. Investigation into possible anomalous activity regarding Researcher Benich has concluded as of 14/05/2017, with negative results. Doctor Benich is not to be informed of the existence of ...
1-Oleoyl-2-(6-((4,4-difluoro-1,3-dimethyl-5-(4-methoxyphenyl)-4-bora-3a,4a-Diaza-s-indacene-2-propionyl)amino)hexanoyl)-sn-glycero-3- ...
Ive heard flip-flops can cause injuries, but kids wear them all summer. How can parents make them as safe as possible? Flip-flops are functional for use around the pool but ideally shouldnt be worn
Buy high quality 2-Acetamido-6-chloro-9-(2,3,5-tri-O-acetyl-β-D-ribofuranosyl)purine 137896-02-7 from toronto research chemicals Inc.
Lookchem Provide Cas No.181282-80-4 Basic information: Properties,Safety Data,Sds and Other Datebase. We also Provide Trading Suppliers & Manufacture for 181282-80-4 Methyl 4-chloro-3-cyanobenzoate.
Buy Diazole (metronidazole) 400mg, 200mg online without prescription in USA, Canada, Australia, UK and Europe. Fast order delivery. Worldwide shipping. FDA approved RX online pharmacy.
Principal Investigator:YAMADA Kazuhiro, Project Period (FY):1993 - 1995, Research Category:Grant-in-Aid for General Scientific Research (B), Research Field:神経・脳内生理学
Item #: SCP-092. Object Class: Safe. Special Containment Procedures: The three thousand one hundred and twenty-five instances of SCP-092 are to be held in individual cases suitable for containing non-anomalous audio compact discs (CDs), and stored in standard inanimate-object lockers at Site 37. Each instance is to be individually numbered with permanent marker.. Testing of instances of SCP-092 is to be done in soundproof rooms. Only one instance of SCP-092 may be examined at a time. Only D-class personnel are to listen to previously-unexamined instances of SCP-092. Research proposals which involve non-D-class personnel listening to instances of SCP-092 require written approval from site command.. The cadaver of SCP-092-B is not currently considered anomalous except by association, and is preserved in the morgue freezer at Site 19.. Description: SCP-092 is a set of 3125 audio CDs, each labeled The Absolute Absolute Absolute Absolute BEST of The 5th Dimension!!!!!, and marked with the names of ...
This page contains information about Fine Chemicals of 4-chloro-3-(2-chloro-1-hydroxyethyl)phenol. Purchase 4-chloro-3-(2-chloro-1-hydroxyethyl)phenol online
As we know that most of the optical brughteneing agent are ionic in their nature so why do some optical brightener form suspension in water? some important optical brightener agents are: 1.Stilbene 2.Diazole 3. Triazole 4. Coumarins 5.Imidazoles Also submitted: Do optical brighteners contain chromophores ...
2-CHLORO-5-NITROACETOPHENONE 23082-50-0 NMR spectrum, 2-CHLORO-5-NITROACETOPHENONE H-NMR spectral analysis, 2-CHLORO-5-NITROACETOPHENONE C-NMR spectral analysis ect.
Benzene,chloro- 108-90-7 Suppliers,provide Benzene,chloro- 108-90-7 product and the products related with China (Mainland) Benzene,chloro- 108-90-7 HANGZHOU YUNUO CHEMICAL CO.,LTD China (Mainland)
TY - JOUR. T1 - Suppression of Rft1 expression does not impair the transbilayer movement of Man5GlcNAc2-P-P-dolichol in sealed microsomes from yeast. AU - Rush, Jeffrey S.. AU - Gao, Ningguo. AU - Lehrman, Mark A.. AU - Matveev, Sergey. AU - Waechter, Charles J.. PY - 2009/7/24. Y1 - 2009/7/24. N2 - To further evaluate the role of Rft1 in the transbilayer movement of Man5GlcNAc2-P-P-dolichol (M5-DLO), a series of experiments was conducted with intact cells and sealed microsomal vesicles. First, an unexpectedly large accumulation (37-fold) of M5-DLO was observed in Rft1-depleted cells (YG1137) relative to Glc3Man9GlcNAc2-P-P-Dol in wild type (SS328) cells when glycolipid levels were compared by fluorophore-assisted carbohydrate electrophoresis analysis. When sealed microsomes from wild type cells and cells depleted of Rft1 were incubated with GDP-[3H]mannose or UDP-[3H]GlcNAc in the presence of unlabeled GDP-Man, no difference was observed in the rate of synthesis of [3H]Man9GlcNAc2-P-P-dolichol ...
It is known that ceramides can influence the lateral organization in biological membranes. In particular ceramides have been shown to alter the composition of cholesterol and sphingolipid enriched nanoscopic domains, by displacing cholesterol, and forming gel phase domains with sphingomyelin. Here we have investigated how the bilayer content of ceramides and their chain length influence sterol partitioning into the membranes. The effect of ceramides with saturated chains ranging from 4 to 24 carbons in length was investigated. In addition, unsaturated 18:1- and 24:1-ceramides were also examined. The sterol partitioning into bilayer membranes was studied by measuring the distribution of cholestatrienol, a fluorescent cholesterol analogue, between methyl-beta-cyclodextrin and large unilamellar vesicle with defined lipid composition. Up to 15 mol% ceramide was added to bilayers composed of DOPC:PSM:cholesterol (3:1:1), and the effect on sterol partitioning was measured. Both at 23 and 37 degrees C ...
24) A comparative study on fluorescent cholesterol analogs as versatile cellular reporters (2016) Sezgin E, Can FB, Schneider F, Clausen MP, Galiani S, Stanly TA, Waithe D, Colaco A, Honigmann A, Wustner D, Platt F, Eggeling C. J Lipid Res 57: 299-309. 25) Bifunctional Sphingosine for Cell-Based Analysis of Protein-Sphingolipid Interactions (2016) Haberkant P, Stein F, Höglinger D, Gerl MJ, Brügger B, Van Veldhoven PP, Krijgsveld J, Gavin AC, Schultz C ACS Chem Biol 11:222-230. 26) Proteomic dataset of the sea urchin Paracentrotus lividus adhesive organs and secreted adhesive (2016) Lebesgue N, da Costa G, Ribeiro RM, Ribeiro-Silva C, Martins GG, Matranga V, Scholten A, Cordeiro C, Heck AJ, Santos R. Data Brief 7: 1497-1505. 27) Deciphering the molecular mechanisms underlying sea urchin reversible adhesion: A quantitative proteomics approach (2016) Lebesgue N, da Costa G, Ribeiro RM, Ribeiro-Silva C, Martins GG, Matranga V, Scholten A, Cordeiro C, Heck AJ, Santos R J Proteomics 138: ...
An accurate and sensitive reversed-phase high-performance liquid chromatograph-ic method for determination of sertraline in human serum is described using 4-chloro-7-nitrobenzofurazan as pre-column derivatization agent. The drug and an internal standard (azithrimycin) were extracted from serum using a mixture of diethyl ether-chloroform and subjected to the pre-column derivatization with the reagent. Analysis of the resulted derivatives was performed on a Lichrosorb CN (250×4.0 mm) column using a mobile phase composed of methanol and sodium phosphate buffer (0.05 M; pH 3.7) containing 2 ml/lit triethylamine (63:37 v/v). Detector response was monitored at excitation and emission wavelengths of 470 and 537 nm, respectively. The calibration curve was linear over the concentration range of 2 to 640 ng/ml. The lower limits of detection and quantification were 0.5 and 2 ng/ml, respectively. The validation of the analysis was carried out in terms of specificity, sensitivity, linearity, precision,
Our immunocytochemistry results from dissociated rat and toad olfactory sensory neurons and SCs revealed the presence of GLUT3 in their respective apical organelles. This notion was strengthened by the ability of the cilia and dendritic knob to incorporate 2-NBDG, as observed in the rat whole-mount preparation. Because the peripheral olfactory system stays intact in this preparation, it is highly unlikely that the sugar reached the apical structures of the epithelium in any other manner. The cilia exhibited marked 2-NBDG fluorescence, indicating glucose uptake; however, due to their small dimensions the microvilli could not be resolved. The measurements performed in dissociated cells confirmed that both, cilia and microvilli (and also the cell bodies), could accumulate 2-NBDG. Importantly, the GLUT blocker cytochalasin B drastically reduced the uptake of the glucose analog. Together, these results suggest that the apical organelles of the OSNs and SCs possess the capability of transporting ...
A direct continuous fluorescence assay for translocase II MurG based on fluorescence resonance energy transfer (FRET) has been developed using a 6-substituted fluorescent analogue of UDP-N-acetylglucosamine.. ...
0020 ETHANE HEXA- CHLORO- CHLORO- CHLORO- ETHYL- WATER METHYL- BENZENE ETHANE BENZENE UNFLTRD BROMIDE TOTAL TOTAL TOTAL RECOVER TOTAL (UG/L) (UG/L) (UG/L) (UG/L) (UG/L) 34301 34311 34371 34396 34413 <.028 <.120 <.030 <.362 <.148 METHYL TETRA- TRI- METHYL- ENE CHLORO- CHLORO- 1,1-DI- CHLO- CHLO- ETHYL- FLUORO- CHLORO- RIDE RIDE ENE METHANE ETHANE TOTAL TOTAL TOTAL TOTAL TOTAL (UG/L) (UG/L) (UG/L) (UG/L) (UG/L) 34418 34423 34475 34488 34496 <.254 <.382 <.038 <.092 <.066 ETHANE, BENZENE 1,1-DI- 1,1,1- 1,1,2- 1,1,2,2 O-DI- CHLORO- TRI- TRI- TETRA- CHLORO- ETHYL- CHLORO- CHLORO- CHLORO- WATER ENE ETHANE ETHANE WAT UNF UNFLTRD TOTAL TOTAL TOTAL REC REC (UG/L) (UG/L) (UG/L) (UG/L) (UG/L) 34501 34506 34511 34516 34536 <.044 <.032 <.064 <.132 <.048 BENZENE BENZENE BENZENE TRANS- 1,2,4- 1,3-DI- 1,4-DI- 1,2-DI- 1,2-DI- TRI- CHLORO- CHLORO- CHLORO- CHLORO- CHLORO- WATER WATER PROPANE ETHENE WAT UNF UNFLTRD UNFLTRD TOTAL TOTAL REC REC REC (UG/L) (UG/L) (UG/L) (UG/L) (UG/L) 343541 134541 34551 34556 34571 ...
0020 ETHANE HEXA- CHLORO- CHLORO- CHLORO- ETHYL- WATER METHYL- BENZENE ETHANE BENZENE UNFLTRD BROMIDE TOTAL TOTAL TOTAL RECOVER TOTAL (UG/L) (UG/L) (UG/L) (UG/L) (UG/L) (34301) (34311) (34371) (34396) (34413) <.100 <.200 <.100 <.100 <.200 METHYL TETRA- TRI- METHYL- ENE CHLORO- CHLORO- 1,1-DI- CHLO- CHLO- ETHYL- FLUORO- CHLORO- RIDE RIDE ENE METHANE ETHANE TOTAL TOTAL TOTAL TOTAL TOTAL (UG/L) (UG/L) (UG/L) (UG/L) (UG/L) (34418) (34423) (34475) (34488) (34496) <.400 <.200 <.100 <.200 <.100 ETHANE, BENZENE 1,1-DI- 1,1,1- 1,1,2- 1,1,2,2 O-DI- CHLORO- TRI- TRI- TETRA- CHLORO- ETHYL- CHLORO- CHLORO- CHLORO- WATER ENE ETHANE ETHANE WAT UNF UNFLTRD TOTAL TOTAL TOTAL REC REC (UG/L) (UG/L) (UG/L) (UG/L) (UG/L) (34501) (34506) (34511) (34516) (34536) <.200 <.100 <.200 <.200 <.100 BENZENE BENZENE BENZENE TRANS- 1,2,4- 1,3-DI- 1,4-DI- 1,2-DI- 1,2-DI- TRI- CHLORO- CHLORO- CHLORO- CHLORO- CHLORO- WATER WATER PROPANE ETHENE WAT UNF UNFLTRD UNFLTRD TOTAL TOTAL REC REC REC (UG/L) (UG/L) (UG/L) (UG/L) (UG/L) ...
Item #: SCP-1953. Object Class: Safe. Special Containment Procedures: Instances of SCP-1953 may be kept in a standard containment locker at Site 20. Individuals infected with SCP-1953-1 are to be housed in a secure containment cell at Bio-Research Area-17.. Description: SCP-1953 is the collective designation of 26 objects commonly found in bedrooms, such as pillows, blankets, stuffed animals and alarm clocks. SCP-1953-1 refers to an unknown number of bipedal beings measuring 4 cm on average and resembling Periplaneta americana (American cockroach) which appear when a human subject enters REM sleep within 5 meters of an instance of SCP-1953. Evidence suggests instances of SCP-1953-1 are capable of reading and understanding spoken English, but so far have not responded to attempts to communicate.. When such an event occurs, instances of SCP-1953-1 will search for any surrounding human subjects and attempt to enter their mouths. SCP-1953 instances exhibit an unexplained ability to calm living human ...
Flagyl ER. Active Ingredient: Metronidazole. Flagyl ER is used in the place of treating certain bacterial infections of the vagina (bacterial vaginosis).. Flagyl ER taken in the character of known as: Acea, Acuzole, Aldezol, Aldezole, Amebidal, Amevan, Aminidazole, Amobin, Amodis, Amotein, Amotrex, Amrizole, Anabact, Anaerobex, Anaeromet, Anamet, Anazol, Anegyn, Anerobia, Anerozol, Arilin, Aristogyl, Asuzol, Avidal, Bemetrazole, Bi missilor, Biatron, Biozyl, Birodogyl, Buccoval, Camezol, Chemagyl, Clont, Collazole, Colpocin t, Colpofilin, Corsagyl, Cresac, Dazotron, Deflamon, Deprocid, Dequazol, Diazole, Dirozyl, Dumozol, Efectimax, Efloran, Elyzol, Emedal, Entizol, Etron, Etronil, Farnat, Filmet, Fladex, Fladystin, Flagemed, Flagenase, Flagicure, Flagolin, Flagystatin, Flagystatine, Flanizol, Flazol, Flazole, Flegyl, Florazole, Fortagyl, Geloderm, Giardyl, Ginerella, Ginkan, Gnostol, Grinazole, Gynomix, Gynoplix, Gynotran, Imizine, Kilpro, Klion, Klont, Lindoplus, Litagyl, M-zed, Mebadiol, ...
Structure, properties, spectra, suppliers and links for: 7-[(2Z)-3-Chloro-2-buten-1-yl]-8-[(2E)-2-(2-hydroxybenzylidene)hydrazino]-3-methyl-3,7-dihydro-1.
Make a mark! The unmissable Ducati Corse flip-flops cleverly mix taste and comfort while leaving the Ducati Corse logo on the sand behind you. Simple and bold at the same time, they are available in a unisex version.
Structure, properties, spectra, suppliers and links for: Methyl 5-[(1S,2S)-2-chloro-1-{[(4R)-1-methyl-4-propyl-L-prolyl]amino}propyl]-1-thio-β-L-arabinop.
You are viewing an interactive 3D depiction of the molecule [(5R,6R,7S,8S,9R)-6-bromo-11-oxa-9-azatricyclo[BLAH.BLAH.BLAH.BLAH]nonan-8-yl]methanol (C8H12BrNO2) from the PQR.
Looking for TCI AMERICAS 3-Chloro-2,4(3h,5h)-furandione,5g (29MC66)? Graingers got your back. Price:$110.50. Easy ordering & convenient delivery. Log-in or register for your pricing.
Sigma-Aldrich offers Aldrich-411582, 1-Chloro-1-methylsilacyclobutane for your research needs. Find product specific information including CAS, MSDS, protocols and references.
Bruen, Danielle and McColgan, Adam and Delaney, Colm and Florea, Larisa and Diamond, Dermot (2016) Fluorescence Sensing for Non-Invasive and Continuous Glucose Detection. In: American Advanced Materials Congress 2016, 4th-9th Dec 2016, The Caribbean Sea.. Daikuzono, Cristiane and Florea, Larisa and Delaney, Colm and Tesfay, Henok and Morrin, Aoife and Diamond, Dermot and Novais de Oliveira Jr., Osvaldo (2016) Impedance spectroscopy for the detection of monosaccharides using functionalized carbon screen-printed electrodes on paper. In: E-MRS 2016 Fall Meeting, 19 to 22 Sept 2016., Warsaw, Poland.. Bruen, Danielle and Delaney, Colm and Florea, Larisa and Diamond, Dermot (2016) Boronic acid derivatives for indirect fluorescent glucose sensing. In: 3rd Insight Student Conference 2016, 14 Sept. 2016, Dublin City University, Dublin, Ireland.. Tudor, Alexandru and McColgan, Adam and Delaney, Colm and Florea, Larisa and Diamond, Dermot (2016) Functional ionic liquids for solvent sensing. In: The 3rd ...
The steady-state ATPase activity of sarcoplasmic-reticulum (Ca(2+)-Mg2+)-ATPase is inhibited by thapsigargin at a molar ratio of 1:1, with a dissociation constant for thapsigargin estimated to be in the sub-nanomolar range. In the presence of thapsigargin, only a single Ca2+ ion binds to the ATPase. Similarly, addition of thapsigargin to the ATPase incubated in the presence of Ca2+ results in the release of one of the two originally bound Ca2+ ions. As monitored by the fluorescence of nitrobenzo-2-oxa-1,3-diazole-labelled ATPase, thapsigargin appears to shift the transition between E1 and E2 conformations towards E2. Addition of thapsigargin prevents phosphorylation of the ATPase by P(i) and results in a very low steady-state level of phosphorylation of the ATPase by ATP, as observed previously for nonylphenol. ...
Item #: SCP-995. Object Class: Keter. Special Containment Procedures: SCP-995 is to be kept in a pure holy water solution blessed by [DATA EXPUNGED]. The water should be held by 7 decimeters of Pb, also blessed by [DATA EXPUNGED], or any other clergyman above the rank of archbishop. Item should be moved by only Class-D personnel of Catholic belief. Under NO circumstances should the Item be removed from its encasing, and any personnel attempting to remove the casing should be terminated with extreme prejudice. In no case should Item 995 contact SCP-013, SCP-616, SCP-640, or SCP-861, as well as any item related to an XK class end-of-the-world scenario(See Document #995-1).. Description: The visual perception of SCP-995 is impossible to describe as personnel who have seen it have been blinded by what appears to be damage to the occipital lobe. Memory is scarred, and personnel who attempt to remember will pass out from hypertension. One personnel ██████████ ██████ was ...
[4-Chloro-1-butanol] [928-51-8] | Buy and find out price and availability, MSDS, properties of TCIs high quality specialty chemicals.
buy HYDRAZINE,(4-CHLORO-3-FLUOROPHENYL)-,HYDROCHLORIDE (1:1) (cas 299163-41-0), a product for lab from labseeker, ,MDL: MFCD11617228,MF: C6H6ClFN2.ClH
AASraw adalah pengilang untuk pengeluaran pukal (17183-98-1) di bawah peraturan CGMP, dan menyediakan jualan dalam talian, kimia sintetik dan disesuaikan,
Should be a law against some wearing like flip-flops to work and stuff... and no just because its unprofessional its just that some no matter how good they look - their feets are just damn ugly -- sorry had to post this ...
Photophosphorylation in vivo by Chlorobium limicola was inhibited by lipophilic cations and the energy-transfer inhibitors diphenylphosphorylazide, Dio-9, 4-chloro-7-nitrobenzofurazan and chlorhexidene. Membrane-bound ATPase activity was also inhibited by these energy-transfer inhibitors. The formation of a membrane potential was stimulated approximately 1.7-fold on illumination, rising to a value between −110 and −150 mV. The sensitivity of the processes producing this membrane potential to uncouplers, energy-transfer inhibitors and 2-heptyl-4-hydroxyquinoline-N-oxide was measured in the light and the dark.
... aims at providing comprehensive data on 4-chloro-2-methoxyphenylboronic
... aims at providing comprehensive data on 2-chloro-3,5-dinitropyridine market
Item #: SCP-935. Object Class: Euclid. Special Containment Procedures: SCP-935 is kept within a standard containment chamber, located in Site 19. It is to be monitored at all times, and in the event of a demanifestation agents are to begin monitoring all subjects known to carry Genetic Marker Bowe-1A. Genetic testing is to be done on all personnel applying for positions in the Foundation, and those who test positive for Genetic Marker Bowe-1A are to be placed under supervision by Foundation agents. If SCP-935 de-materializes, agents are instructed first to begin surveillance of all subjects carrying Genetic Marker Bowe-1A. Agents will be briefed on how to identify these individuals prior to being assigned to SCP-935s containment. When SCP-935 reappears, it is to be retrieved and returned to containment, with containment procedures being updated.. Description: SCP-935 is a box of playing cards, appearing to date from pre-1700 Germany, specifically Brandenburg. All cards composing SCP-935 are ...
chemBlink provides information about CAS # 51527-19-6, 2-(5-Chloro-3-methylbenzo[b]thiophen-2-yl)acetic acid, molecular formula: C11H9ClO2S.
Product Name: 1H-Pyrazole-4-carboxylic acid, 5-(aminosulfonyl)-3-chloro-1-methyl-, methyl ester. CAS RN: 100784-27-8. Spec.: colorless fine flaky crystal ...
Srinivasan, T.; Yuvaraj, P.; Reddy, B.S.R.; Velmurugan, D., 2013: 2-Chloro-8,8-dimethyl-8,9-dihydro-7H-chromeno[2,3-b]quinoline-10,12-dione
The nematode Caenorhabditis elegans is a genetically tractable model organism to investigate sterol transport. In vivo imaging of the fluorescent sterol, dehydroergosterol (DHE), is challenged by C. elegans high autofluorescence in the same spectral region as emission of DHE. We present a method to detect DHE selectively, based on its rapid bleaching kinetics compared to cellular autofluorescence. Worms were repeatedly imaged on an ultraviolet-sensitive wide field (UV-WF) microscope, and bleaching kinetics of DHE were fitted on a pixel-basis to mathematical models describing the intensity decay. Bleach-rate constants were determined for DHE in vivo and confirmed in model membranes. Using this method, we could detect enrichment of DHE in specific tissues like the nerve ring, the spermateca and oocytes. We confirm these results in C. elegans gut-granule-loss (glo) mutants with reduced autofluorescence and compare our method with three-photon excitation microscopy of sterol in selected tissues. Bleach
The nematode Caenorhabditis elegans is a genetically tractable model organism to investigate sterol transport. In vivo imaging of the fluorescent sterol, dehydroergosterol (DHE), is challenged by C. elegans high autofluorescence in the same spectral region as emission of DHE. We present a method to detect DHE selectively, based on its rapid bleaching kinetics compared to cellular autofluorescence. Worms were repeatedly imaged on an ultraviolet-sensitive wide field (UV-WF) microscope, and bleaching kinetics of DHE were fitted on a pixel-basis to mathematical models describing the intensity decay. Bleach-rate constants were determined for DHE in vivo and confirmed in model membranes. Using this method, we could detect enrichment of DHE in specific tissues like the nerve ring, the spermateca and oocytes. We confirm these results in C. elegans gut-granule-loss (glo) mutants with reduced autofluorescence and compare our method with three-photon excitation microscopy of sterol in selected tissues. Bleach
Inflammation is associated with the development of insulin resistance in Type 2 diabetes mellitus. In the present study, mouse FL83B cells were treated with tumor necrosis factor-alpha (TNF-α) to induce insulin resistance, and then co-incubated with a fraction from wax apple fruit extract (FWFE). This fraction significantly increased the uptake of the nonradioactive fluorescent indicator 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-d-glucose (2-NBDG) in insulin resistant cells. Western blot analysis revealed that, compared with the TNF-α-treated control group, FWFE increased the expression of the insulin receptor (IR), insulin receptor substrate-1 (IRS-1), protein kinase B (Akt/PKB), phosphatidylinositol-3 kinase (PI3K), and glucose transporter 2 (GLUT-2), and increased IR tyrosyl phosporylation, in insulin resistant FL83B cells. However, FWFE decreased phosphorylation of c-Jun N-terminal kinases (JNK), but not the expression of the intercellular signal-regulated kinases (ERK), in the same
BioAssay record AID 416393 submitted by ChEMBL: Inhibition of human MMP1 catalytic domain at 50 uM after 1 hr by fluorescent substrate assay.
ChemicalBook provide Chemical industry users with 2-CHLORO-8-METHYL-3-QUINOLINE CARBOXALDEHYDE(73568-26-0) Boiling point Melting point,2-CHLORO-8-METHYL-3-QUINOLINE CARBOXALDEHYDE(73568-26-0) Density MSDS Formula Use,If You also need to 2-CHLORO-8-METHYL-3-QUINOLINE CARBOXALDEHYDE(73568-26-0) Other information,welcome to contact us.
Alfa Aesar™ 1-(2-Chloro-6-nitrophenyl)piperazine, 97% 5g Alfa Aesar™ 1-(2-Chloro-6-nitrophenyl)piperazine, 97% Chloronitrod to Chlorophenyla...
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy ...
96122-36-0 - ZKSGBNQSDRTERE-SPIKMXEPSA-N - Piperazine, 1-(2-((2-chloro-6,11-dihydrodibenzo(b,e)thiepin-11-yl)oxy)ethyl)-, (Z)-2-butenedioate (1:2) - Similar structures search, synonyms, formulas, resource links, and other chemical information.
3-Chloro-4-ethoxy-N-furan-2-ylmethyl-benzamide | C14H14ClNO3 | CID 881556 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
1-phenyl-3-dimethylamino-6-chloro-7-hydroxy-1,2,3,4-tetrahydronaphthalene: RN refers to (trans)-isomer; a phenylaminotetralin; structure given in first source
5-tetrahydro-8-chloro-3-methyl-5-phenyl-1h-3-benzazepin-7-ol MeSH D03.438.079.800 --- 2,3,4,5-tetrahydro-7,8-dihydroxy-1-phenyl ... 8-chloro-, 2-acetylhydrazide MeSH D03.494.347.500 --- loxapine MeSH D03.494.347.500.040 --- amoxapine MeSH D03.494.382.393 --- ... 4,5-dihydro-1-(3-(trifluoromethyl)phenyl)-1h-pyrazol-3-amine MeSH D03.383.129.539.200 --- epirizole MeSH D03.383.129.539.487 ... 4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, methyl ester MeSH D03.383.725.210 --- dimethindene MeSH D03.383. ...
4-Chloro-7-nitrobenzofurazan is used as a derivatizing reagent for chromatography analysis of amino acids and low molecular ... It is utilized in the preparation of fluorescent phospholipid-derivative, hydroxynaphthofurazan and 4-chloro-7- ... nitrobenzofurazan- didecanoylphosphatidylethanolamine. It serves as a fluorescent reagent to label free sulfhydryls and N- ... functionalized hydroxynaphthofurazan and 7-nitrobenzofurazan (NBD)-labeled maleimide. ...
Learn more about 4-chloro-7-nitrobenzofurazan. We enable science by offering product choice, services, process excellence and ...
2-Chloro-4-nitrophenol, 97%. ×Close. 2-Chloro-4-nitrophenol, 97% Structure ... 4-(2-Pyridylazo)resorcinol monosodium salt monohydrate. ×Close. 4-(2-Pyridylazo)resorcinol monosodium salt monohydrate ...
Novel application of 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole to identify cysteine sulfenic acid in the AhpC component of alkyl ... As shown in Figure 7, only the addition of the oxidized CosR into the Trx system showed a consumption of NADPH. No reduction in ... The formation of Cys-SOH in CosR variants is measured by the 4-chloro-7-nitrobenzofurazan (NBD-Cl) labeling assay [31-33]. ... The formation of disulfide bond and sulfenic acid in CosR WT and its variants are performed with the thiol-reactive probe 4- ...
2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose ...
I agree Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our website without changing the browser settings you grant us permission to store that information on your device. ...
7-Methoxycoumarin-3-carboxylic acid N-succinimidyl ester. MeOH. 358. 410. 64958. 2-Methoxy-2,4-diphenyl-3(2H)-furanone. Amine ... 7-Hydroxy-4-methyl-3-coumarinylacetic acid. pH 10.0. 360. 445. 55627. 7-Hydroxy-4-methyl-2(1H)-quinolone. pH 5.0. 321. 357. pH ... 4-Chloro-7-nitrobenzofurazan. MeOH. 336. Aliphatic amine adducts. 475. 540. 29059. 1,3-Cyclohexanedione. Protein adduct. 375. ... 7-(Diethylamino)coumarin-3-carbonyl azide. MeOH. 432. 480. 36799. 7-(Diethylamino)coumarin-3-carboxylic acid. pH 9.0. 409. 473 ...
4 A and D, and of 4ASU for Fig. 4B that were simplified from SI Appendix, Fig. S1 to emphasize mechanistic features. Several ... 4). As the catalytic dwell ends (Fig. 4A), catalytic site βD hydrolyzed ATP to Pi and ADP. Behind subunit γ, catalytic site βT ... 4, ) to enable tight winding that can store elastic energy. In all F1 structures known to date, subunit γ is tethered to the ( ... 4C) is thought to result from force applied to subunit γ as a crankshaft from ATP binding-dependent closure βE-lever (i.e., the ...
Cells were labeled with 4 μM C6NBD-GlcCer or C6-NBD-SM at 37°C for 30 min. ... Cells were labeled with 4 μM C6NBD-GlcCer or C6-NBD-SM at 37°C for 30 min. Both lipid analogues were present in the BC ( ... Cells were labeled with 4 μM C6NBD-GlcCer or C6-NBD-SM at 37°C for 30 min. Both lipid analogues were present in the BC ( ... As a control, cells were incubated in HBSS in the absence of sodiumdithionite at 4°C for 7 min. After extensively washing (15 ...
Description: the structure of bovine f1-atpase covalently inhibited with 4-chloro-7-nitrobenzofurazan. Deposited on 1998-04-30 ...
Synthesis of 7-nitrobenzofurazan (NBD)-labeled maleimide, via Diels-Alder reaction. NBD-Cl is widely used to label peptides, ... 4-Chloro-7-nitrobenzofurazan (NBD-chloride) was used in the following studies, Synthesis of fluorescent phospholipid-derivative ... Utility of 4-chloro-7-nitrobenzofurazan (NBD-CI) for the Spectrophotometric and spectrofluorometric determination of several ...
4 (a) SEM micrograph of the anode non-woven carbon mat coated with PVFc CNT with details of coated and uncoated areas. (b and c ... Figure 4. XRD pattern of ash mixed with MS and SS under different conditions (a: MS; b: SS; c: M9S1; and d: M8S2). (1) SiO2, (2 ... Figure 7. Scatter plots of the cell performance against αCO, xCO2in, and L in (a c) the IND scenario (circles) and (d f) the ... Table 4: For the next few graphics, there is a parameter called ICP for Indicator of Comprehensive Performance. There are ...
Quantitative analysis of sertraline in human serum by LC with fluorescence detection after pre-column derivatization with 4- ... chloro-7-nitrobenzofurazan. Bahrami, G; Mohammadi, B; Farshchi, A; Ghiasi, G. * Development of a sensitive liquid-liquid ...
Similarly, chloro drug derivative of diltiazem was formed by means of reaction involving tertiary amino group of diltiazem and ... the 8-chloro derivative of diltiazem (clentiazem maleate) and their deacetyl forms were resolved on ovomucoid-bonded chiral ... "Direct high-performance liquid chromatographic separation of the enantiomers of diltiazem hydrochloride and its 8-chloro ... Nighat Shafi,1 Farhan Ahmed Siddiqui,1 Huma Naseem,2 Nawab Sher,3 Arif Zubair,4 Azhar Hussain,5 Ali Akbar Sial,1 and Mirza ...
CAS: 4197-24-4 Molecular Formula: C21H22ClN3 Molecular Weight (g/mol): 351.878 MDL Number: MFCD00143923 InChI Key: ... 4-Chloro-7-nitrobenzofurazan, 4-Chloro-7-nitrobenzo-2-oxa-1, 3-diazole PubChem CID: 25043 ChEBI: CHEBI:78878 ... 7-Hydroxy-3H-phenoxazin-3-one 10-oxide, sodium salt PubChem CID: 112939 ...
5-tetrahydro-8-chloro-3-methyl-5-phenyl-1h-3-benzazepin-7-ol MeSH D03.438.079.800 --- 2,3,4,5-tetrahydro-7,8-dihydroxy-1-phenyl ... 8-chloro-, 2-acetylhydrazide MeSH D03.494.347.500 --- loxapine MeSH D03.494.347.500.040 --- amoxapine MeSH D03.494.382.393 --- ... 4,5-dihydro-1-(3-(trifluoromethyl)phenyl)-1h-pyrazol-3-amine MeSH D03.383.129.539.200 --- epirizole MeSH D03.383.129.539.487 ... 4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, methyl ester MeSH D03.383.725.210 --- dimethindene MeSH D03.383. ...
4. The variations in I50 values for efrapeptin closely followed changes in specific activity of ATPase, as would be expected ... 4-chloro-7-nitrobenzofurazan, quercetin and spegazzinine, all of which show different sensitivity profiles from one another. 5 ...
FIG 4 B22 enters the cytoplasm and induces membrane blebbing in regions of active peptidoglycan turnover. (A) Time-lapse phase- ... Initial attachment (no flow) proceeded for 30 min followed by a flow rate of 0.5 ml min−1 for 4 h in medium containing a 20,000 ... FIG 7 B22 induces oxidative stress, cell shortening, and membrane blebbing. (A) Time-lapse phase-contrast and fluorescent image ... One milliliter of culture was centrifuged at 14,000 × g for 5 min at 4°C. Cell pellets were washed once with cold medium, ...
4 CRL Guidance Paper . -CRLs view on state of the art analytical methods for the national residue control plans; ( 2007 ); http ... 4 CRL Guidance Paper . -CRLs view on state of the art analytical methods for the national residue control plans; ( 2007 ); http ... Equipment and instruments Kinetex XB C-18 HPLC column (100 mm × 3 mm, 2.6 μm) and C-18 security guard column (4 mm × 2 mm); ... Equipment and instruments Kinetex XB C-18 HPLC column (100 mm × 3 mm, 2.6 μm) and C-18 security guard column (4 mm × 2 mm); ...
GLUTATHIONE-S-TRANSFERA 1-Chloro-2,4-Dinitrobenzene as SubstrateSE -Enzymatic Assay Protocol ...
Prokai, L., Guo, J. & Prokai-Tatrai, K., 1 Jan 2014, In : Nature Protocols. 9, 4, p. 882-895 14 p.. Research output: ... Nguyen, V., Zharikova, A. D., Prokai-Tatrai, K. & Prokai, L., 1 Apr 2010, In : Brain Research Bulletin. 82, 1-2, p. 83-86 4 p. ... Rauniyar, N., Prokai-Tatrai, K. & Prokai, L., 1 Apr 2010, In : Journal of Mass Spectrometry. 45, 4, p. 398-410 13 p.. Research ... Prokai, L., Szarka, S., Wang, X. & Prokai-Tatrai, K., 6 Apr 2012, In : Journal of Chromatography A. 1232, p. 281-287 7 p.. ...
UNITED STATES AGENCY FOR HEALTH CARE POLICY AND RESEARCH ...
During repetitive stimulation of skeletal muscle, extracellular ATP levels raise, activating purinergic receptors, increasing Ca2+ influx, and enhancing contractile force, a response called potentiation. We found that ATP appears to be released through pannexin1 hemichannels (Panx1 HCs). Immunocytochemical analyses and function were consistent with pannexin1 localization to T-tubules intercalated with dihydropyridine and ryanodine receptors in slow (soleus) and fast (extensor digitorum longus, EDL) muscles. Isolated myofibers took up ethidium (Etd+) and released small molecules (as ATP) during electrical stimulation. Consistent with two glucose uptake pathways, induced uptake of 2-NBDG, a fluorescent glucose derivative, was decreased by inhibition of HCs or glucose transporter (GLUT4), and blocked by dual blockade. Adult skeletal muscles apparently do not express connexins, making it unlikely that connexin hemichannels contribute to the uptake and release of small molecules. ATP release, Etd+ ...
Although the flavonoid tangeretin (5, 6, 7, 8, 4-pentamethoxyflavone) is known to possess beneficial health effects, the anti- ... 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose ...
2012;7:e32603 pubmed publisher ..The possibility to study separate and dynamic interactions between SNARE proteins, complexin ... 4 chloro 7 nitrobenzofurazan*filipin*snare proteins*freeze fracturing*seminal plasma proteins*sphingomyelins*phase contrast ...
  • For this, the cells were labeled with fluorescent acyl chain-labeled 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-amino]hexanoic acid (C6-NBD) derivatives of either GlcCer (C6-NBD-GlcCer) or SM (C6-NBD-SM). (nih.gov)
  • 4-Chloro-7-nitrobenzofurazan (NBD-chloride) was used in the following studies, Synthesis of fluorescent phospholipid-derivative, NBD-didecanoylphosphatidylethanolamine, Synthesis of functionalized hydroxynaphthofurazan, Spectrophotometric and spectrofluorometric determination of clemastine hydrogen fumarate, loratadine, losartan potassium and ramipril in pharmaceutical formulations. (alfa.com)
  • Expression data for sodium/iodide symporter (solute carrier family 5 member 5, SLC5A5), hexokinase (HK1-3), glucose-6-phosphate dehydrogenase (G6PD), and glucose transporter (solute carrier family 2, SLC2A1-4) mRNA were collected. (bvsalud.org)
  • In addition, the plasma glucose and triglyceride levels increased significantly after 4 weeks of a high-fat diet. (bvsalud.org)
  • Diltiazem hydrochloride (DLZ), d-cis diltiazem, d-cis-3-acetyloxy-5-[2-(dimethylamino) ethyl]-2,3-dihydro-2-(4-methoxyphenyl)-1,5-benzothiazepin-4(5H)one)hydrochloride (Figure 1 ) are one of the widely used benzothiazepine Ca 2+ -blocking drugs (calcium channel blockers). (hindawi.com)
  • Characterization of inositol 1,4,5-trisphosphate-sensitive (IsCaP) and -insensitive (IisCaP) nonmitochondrial Ca2+ pools in rat pancreatic acinar cells. (ox.ac.uk)
  • Using inhibitors of known action, such as the H+ ATPase inhibitors NBD-Cl and NEM, the Ca2+ ATPase inhibitor vanadate as well as the second messenger inositol 1,4,5-trisphosphate (IP3) and its analog inositol 1,4,5-trisphosphorothioate (IPS3), we could functionally differentiate two nonmitochondrial Ca2+ pools. (ox.ac.uk)
  • Sertraline hydrochloride [(cis-(1S,4S)-N-methyl-4 (3,4-dichlorophenyl)-1,2,3,4-tetrahydro-1 naphthalenamine)] is a relatively new selective serotonin reuptake inhibitor (SSRI) which is administrated in treatment of depression, panic disorder, anxiety and social phobia . (ijps.ir)
  • The present study was aimed to develop an accurate and sensitive method to measure sertraline in human serum, using 4-chloro-7-nitrobenzofurazan (NBD-Cl) as labeling agent and test its applicability for analysis of the drug in a healthy volunteers following single dose administration. (ijps.ir)
  • Both methods are based on coupling with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in borate buffer of pH 7.8 and measuring the reaction product spectrophotometrically at 395 nm (Method I) or spectrofluorimetrically at 530 nm upon excitation at 480 nm (Method II). (biomedcentral.com)
  • Addition of sulfhydryl groups to Escherichia coli ribosomes by protein modification with 2-iminothiolane (methyl 4-mercaptobutyrimidate). (naver.com)
  • C. glutamicum cells are frequently confronted with excessive reactive oxygen species (ROS) production, triggering sudden changes of fermentative conditions in temperature, pH, osmotic pressure, or toxic compounds [ 4 - 5 ]. (portlandpress.com)
  • Cells were labeled with 4 μM C6NBD-GlcCer or C6-NBD-SM at 37°C for 30 min. (nih.gov)
  • Note that in order to visualize intracellular fluorescence in b and d, basolateral membrane-located lipid analogues were partly removed by a brief back exchange procedure (5-10 min, 4°C). Alternatively, cells incubated as in a and c were subsequently subjected to a back exchange procedure at 4°C for 30 min to completely remove the basolateral PM pool of lipid analogues. (nih.gov)
  • After back exchange, the cells were incubated in HBSS, supplemented with 30 mM sodiumdithionite at 4°C for 7 min. (nih.gov)
  • Histamine H 2 receptor antagonists: cimetidine (CIM), ranitidine (RAN), and famotidine (FAM) classified as class III drugs (high solubility, low permeability) according to the Biopharmaceutics Classification System (BCS) [ 3 , 4 ] are used in the treatment of gastrooesophageal reflux disease and gastric and duodenal ulceration [ 5 ]. (hindawi.com)
  • Cathelicidins are thought to interact with each other within the membranes of susceptible bacteria to form assemblages that affect permeability and result in cell death ( 4 ). (asm.org)
  • This methods, however, are not sensitive enough to measure low drug levels which are obtained in human single dose studies [3-5, 7], or need an expensive instrument which is not available in many laboratories [6-9, (ijps.ir)
  • Cytokine array analysis revealed a remarkable increase in the expression of both CXCL12 and CXCL13, whereas the proinflammatory cytokines remained low after 4 weeks of a high-fat diet. (bvsalud.org)
  • Although the flavonoid tangeretin (5, 6, 7, 8, 4'-pentamethoxyflavone) is known to possess beneficial health effects, the anti-diabetic effects and the mechanism of action have not been elucidated. (nih.gov)
  • CONCLUSION: A significant increase in plasma levels of CXCL12 and CXCL13 was observed after 4 weeks of a high-fat diet, which might induce the migration of B lymphocytes, T lymphocytes, and monocytes from the blood to expanding adipose tissue or fat associated lymphoid clusters, playing a key role in adipose tissue remodeling and local immunity during the early stages of high-fat diet-induced obesity. (bvsalud.org)
  • The genital fat depot was enlarged along with an increase in the number and the mean size of white adipocytes as early as 4 weeks after a high-fat diet. (bvsalud.org)