A non-penetrating amino reagent (commonly called SITS) which acts as an inhibitor of anion transport in erythrocytes and other cells.
An inhibitor of anion conductance including band 3-mediated anion transport.
A class of organic compounds that contains a naphthalene moiety linked to a sulfonic acid salt or ester.
A subclass of purinergic P2 receptors that signal by means of a ligand-gated ion channel. They are comprised of three P2X subunits which can be identical (homotrimeric form) or dissimilar (heterotrimeric form).
Compounds that bind to and block the stimulation of PURINERGIC P2 RECEPTORS.
This is the active form of VITAMIN B 6 serving as a coenzyme for synthesis of amino acids, neurotransmitters (serotonin, norepinephrine), sphingolipids, aminolevulinic acid. During transamination of amino acids, pyridoxal phosphate is transiently converted into pyridoxamine phosphate (PYRIDOXAMINE).
A class of cell surface receptors for PURINES that prefer ATP or ADP over ADENOSINE. P2 purinergic receptors are widespread in the periphery and in the central and peripheral nervous system.
A polyanionic compound with an unknown mechanism of action. It is used parenterally in the treatment of African trypanosomiasis and it has been used clinically with diethylcarbamazine to kill the adult Onchocerca. (From AMA Drug Evaluations Annual, 1992, p1643) It has also been shown to have potent antineoplastic properties.
A colorimetric reagent for iron, manganese, titanium, molybdenum, and complexes of zirconium. (From Merck Index, 11th ed)
A group of broad-spectrum antibiotics first isolated from the Mediterranean fungus ACREMONIUM. They contain the beta-lactam moiety thia-azabicyclo-octenecarboxylic acid also called 7-aminocephalosporanic acid.
Organic compounds that contain 1,2-diphenylethylene as a functional group.
Inorganic compounds derived from hydrochloric acid that contain the Cl- ion.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
One of the CEPHALOSPORINS that has a broad spectrum of activity against both gram-positive and gram-negative microorganisms.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Inorganic salts that contain the -HCO3 radical. They are an important factor in determining the pH of the blood and the concentration of bicarbonate ions is regulated by the kidney. Levels in the blood are an index of the alkali reserve or buffering capacity.
Derivatives of acetamide that are used as solvents, as mild irritants, and in organic synthesis.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
The N-acetyl derivative of galactosamine.
Spectrophotometry in the infrared region, usually for the purpose of chemical analysis through measurement of absorption spectra associated with rotational and vibrational energy levels of molecules. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The N-acetyl derivative of glucosamine.
Membrane transporters that co-transport two or more dissimilar molecules in the opposite direction across a membrane. Usually the transport of one ion or molecule is against its electrochemical gradient and is "powered" by the movement of another ion or molecule with its electrochemical gradient.
The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.
The characteristic 3-dimensional shape of a carbohydrate.
Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.
Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
The giving of drugs, chemicals, or other substances by mouth.
The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
The rate dynamics in chemical or physical systems.
Substances that reduce the growth or reproduction of BACTERIA.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Nucleic acid which complements a specific mRNA or DNA molecule, or fragment thereof; used for hybridization studies in order to identify microorganisms and for genetic studies.
DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.
Exclusive legal rights or privileges applied to inventions, plants, etc.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
A proteolytic enzyme in the serine protease family found in many tissues which converts PLASMINOGEN to FIBRINOLYSIN. It has fibrin-binding activity and is immunologically different from UROKINASE-TYPE PLASMINOGEN ACTIVATOR. The primary sequence, composed of 527 amino acids, is identical in both the naturally occurring and synthetic proteases.
A product of the lysis of plasminogen (profibrinolysin) by PLASMINOGEN activators. It is composed of two polypeptide chains, light (B) and heavy (A), with a molecular weight of 75,000. It is the major proteolytic enzyme involved in blood clot retraction or the lysis of fibrin and quickly inactivated by antiplasmins.
A proteolytic enzyme that converts PLASMINOGEN to FIBRINOLYSIN where the preferential cleavage is between ARGININE and VALINE. It was isolated originally from human URINE, but is found in most tissues of most VERTEBRATES.
Precursor of plasmin (FIBRINOLYSIN). It is a single-chain beta-globulin of molecular weight 80-90,000 found mostly in association with fibrinogen in plasma; plasminogen activators change it to fibrinolysin. It is used in wound debriding and has been investigated as a thrombolytic agent.
A serine proteinase inhibitor used therapeutically in the treatment of pancreatitis, disseminated intravascular coagulation (DIC), and as a regional anticoagulant for hemodialysis. The drug inhibits the hydrolytic effects of thrombin, plasmin, and kallikrein, but not of chymotrypsin and aprotinin.
A member of the serpin family of proteins. It inhibits both the tissue-type and urokinase-type plasminogen activators.
A heterogeneous group of proteolytic enzymes that convert PLASMINOGEN to FIBRINOLYSIN. They are concentrated in the lysosomes of most cells and in the vascular endothelium, particularly in the vessels of the microcirculation.
... isothiocyanatostilbene-2,2-disulfonic acid sodium salt, fluorescent marker (ab144879). ... 4-acetamido-4-isothiocyanatostilbene-22-disulfonic-acid-sodium-salt-fluorescent-marker-ab144879.pdf ... 4-Acetamido-4-isothiocyanatostilbene-2,2-disulfonic acid sodium salt, fluorescent marker ...
The systems and methods described herein also relate to apparatus for sequencing nucleic acids which include gapped probes, as ... and the use of sets of gapped probes in sequencing by hybridization to determine the sequence of nucleic acid sequences. The ... The systems and methods described herein relate to nucleic acid probes comprising a a pattern of universal and designate ... disulfonic acid; pyrene-3-sulfonic acid; 2-toluidinonaphthalene-6-sulfonate; N-phenyl-N-methyl-2-aminoaphthalene-6-sulfonate; ...
... disulfonic Acid / analogs & derivatives * 4-Acetamido-4-isothiocyanatostilbene-2,2-disulfonic Acid / pharmacology ... isothiocyanatostilbene-2,2- ... its preferential site of action and that of iodoacetic acid ... acetate exhibits a much lower capacity for cell entry and that the structural analog alpha-cyano-3-hydroxycinnamic acid ...
The primary sequence, composed of 527 amino acids, is identical in both the naturally occurring and synthetic proteases. ... disulfonic Acid (SITS) Related Therapies and Procedures. 1. Thrombolytic Therapy 2. Catheters ... Acetamido- 4- isothiocyanatostilbene- 2,2- ... The primary sequence, composed of 527 amino acids, is identical ... Amino Acids, Peptides, and Proteins*Proteins: 90489*Blood Proteins: 4303*Blood Coagulation Factors: 2147*Plasminogen Activators ...
... disulfonic acid, 4-acetamido-4-isothiocyanato-stilbene-2,2-disulfonic acid, 4,4-diisothiocyanatostilbene-2,2-disulfonic ... disulfonic acid4-acetamido-4-isothiocyanato- 336 438 Mstilbene-2,2-disulfonic acid4,4-Diisothiocyanatostilbene 342 419 M2,2 ... Target nucleic acid sequences to be hybridized in the present methods can be present in any nucleic acid-containing sample so ... For instance, nucleic acids can be isolated from a variety of nucleic acid-containing samples including body tissue, such as ...
... disulfonic acid known to block Cl- conductances. It is suggested the the voltage-sensitive dye signals recorded from the ... acetamido-4isothiocyanatostilbene-2,2′- ...
... disulfonic acid (SITS), indicating the absence of SITS-sensitive HCO3(-)-dependent mechanisms. Recovery instead appeared mostly ... isothiocyanatostilbene-2,2- ... using the pH-sensitive dye 2,7-bis(2-carboxyethyl)-5(6)- ... Recovery of pHi was dependent on extracellular Na+ (Km, 13.1 mM); however, it was not attenuated by 4-acetamido-4- ...
The channel is blocked by carboxylic acid derivatives and Cd2+ but is largely unaffected by disulfonic stilbene derivatives and ... isothiocyanatostilbene-2,2′-disulfonic acid (SITS) have been identified as effective blockers of a variety of cardiac5 38 39 ... Several compounds including arylaminoalkyl benzoates derivatives such as 9-anthracene-carboxylic acid (9-AC) and disulfonic ... A, Carboxylic acid derivative 9-AC blocked ICl.ir in mouse atrial myocytes. A-a, Mean I-V curves of ICl.ir obtained from 4 ...
... disulfonic acid (DIDS) produced a permanent block. The channel was rarely active in cell-attached patches and usually required ... isothiocyanatostilbene-2,2′-disulfonic acid (SITS), while 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS) produced a ... Add Journal to My Library The Journal of Membrane Biology , Volume 158 (2) - Jul 15, 1997 ... A flickery, reversible block was produced by the diuretic stilbene 4-acetamido-4′-isothiocyanatostilbene-2,2′-disulfonic acid ( ...
... disulfonic acid (DIDS). The haemolysis caused by mannan was inhibited by DIDS, SITS (4-acetamido-4′-isothiocyanatostilbene-2,2 ... disulfonic acid) and bis(sulfosuccinimidyl) suberate, but not by pyridoxal 5-phosphate. These results indicated that a ... isothiocyanatostilbene-2,2′-disulfonic acid) and bis(sulfosuccinimidyl) suberate, but not by pyridoxal 5-phosphate. These ... disulfonic acid (DIDS). The haemolysis caused by mannan was inhibited by DIDS, SITS (4-acetamido-4′- ...
Sialic acid and sodium sulfate, which are transported only very slowly into the lumen of Golgi vesicles, are transported at low ... The resulting proteoliposomes transport CMP-N-acetylneuraminic acid (CMP-AcNeu) and adenosine 3-phosphate 5-phosphosulfate ... Disulfonic Acid; 4-Acetamido-4-isothiocyanatostilbene-2,2-disulfonic Acid; derivatives; Adenine Nucleotides; Animals; ... Sialic acid and sodium sulfate, which are transported only very slowly into the lumen of Golgi vesicles, are transported at low ...
DISULFONIC ACID. DOM. 2,5-DIMETHOXY-4-METHYLAMPHETAMINE. EIMERIINA. EIMERIIDA. EPN. PHENYLPHOSPHONOTHIOIC ACID, 2-ETHYL 2-(4- ... ISOTHIOCYANATOSTILBENE-2,2-DISULFONIC ACID. SPOROZOEA. APICOMPLEXA. SPOROZOEA INFECTIONS. PROTOZOAN INFECTIONS. TYPHOID. ...
... disulfonic Acid * National Institute of Neurological Disorders and Stroke (U.S.) ... isothiocyanatostilbene-2,2- ... Impact of long-term gastric acid suppression on spontaneous ... Efficacy of phenytoin, valproic acid, carbamazepine and new antiepileptic drugs on control of late-onset post-stroke epilepsy ... Ko, T. H., Chiu, W. T., Chao, Y. T. & Chan, L., Jan 1 2016, In : Neurology India. 64, 1, p. 164-165 2 p.. Research output: ...
Ohkuma, M., Kawai, F., Horiguchi, M. & Miyachi, E. I., 03-2007, In: Photochemistry and Photobiology. 83, 2, p. 317-322 6 p.. ... Kawai, F., Horiguchi, M., Suzuki, H. & Miyachi, E. I., 2001, In: Neuron. 30, 2, p. 451-458 8 p.. Research output: Contribution ... Kawai, F., 2002, In: Biophysical Journal. 82, 4, p. 2005-2015 11 p.. Research output: Contribution to journal › Article › peer- ... Kawai, F. & Miyachi, E. I., 08-09-2000, In: Brain Research. 876, 1-2, p. 180-184 5 p.. Research output: Contribution to journal ...
... disulfonic acid, and rhodamine B isothiocyanate for the characterization and identification of microorganisms was tested with ... The suitability of using binary mixtures of 1-pyrene butyric acid, 3,6-dimethylamino acridine, 4-acetamido-4′- ... isothiocyanatostilbene-2,2′-disulfonic acid, and rhodamine B isothiocyanate for the characterization and identification of ... The suitability of using binary mixtures of 1-pyrene butyric acid, 3,6-dimethylamino acridine, 4-acetamido-4′- ...
... disulfonic Acid Medicine & Life Sciences * National Institute of Neurological Disorders and Stroke (U.S.) Medicine & Life ... isothiocyanatostilbene-2,2- ... increased the risk of the occurrence of SICH by nearly 2-fold ( ... Admission hyperglycemia increased the risk of the occurrence of SICH by nearly 2-fold (adjusted RR: 1.891 [0.977-3.657], p = ... Admission hyperglycemia increased the risk of the occurrence of SICH by nearly 2-fold (adjusted RR: 1.891 [0.977-3.657], p = ...
... disulfonic acid (SITS; 1 mM), and disodium 4,4-diisothiocyanatostilbene-2,2-disulfonate (DIDS; 1 mM), but only DIDS produced ... isothiocyanatostilbene-2,2-disulfonic acid (SITS; 1 mM), and disodium 4,4-diisothiocyanatostilbene-2,2-disulfonate (DIDS; 1 ... disulfonic acid (SITS; 1 mM), and disodium 4,4-diisothiocyanatostilbene-2,2-disulfonate (DIDS; 1 mM), but only DIDS produced ... disulfonic acid (SITS; 1 mM), and disodium 4,4-diisothiocyanatostilbene-2,2-disulfonate (DIDS; 1 mM), but only DIDS produced ...
... disulfonic Acid , Asian Continental Ancestry Group , Haplotypes , HapMap Project , Humans , Polymorphism, Single Nucleotide ... isothiocyanatostilbene-2,2'-disulfonic Acid / Haplotypes / Humans / Polymorphism, Single Nucleotide / Asian Continental ... disulfonic Acid / Haplotypes / Humans / Polymorphism, Single Nucleotide / Asian Continental Ancestry Group / HapMap Project ... Full text: Available Index: WPRIM (Western Pacific) Main subject: 4-Acetamido-4'-isothiocyanatostilbene-2,2'- ...
Short-Term Ascorbic Acid Deficiency Induced Oxidative Stress in the Retinas of Young Guinea Pigs. Ohta, Y., Okubo, T., Niwa, T ... Prolonged marginal ascorbic acid deficiency induces oxidative stress in retina of guinea pigs. Ohta, Y., Okubo, T., Niwa, T., ... Kachi, S. & Hirano, K., 08-05-2002, In: Cornea. 21, 4, p. 421-423 3 p.. Research output: Contribution to journal › Article › ... Kawai, F., Horiguchi, M., Suzuki, H. & Miyachi, E. I., 2001, In: Neuron. 30, 2, p. 451-458 8 p.. Research output: Contribution ...
... disulfonic Acid , Anastomotic Leak , Animals , Capillaries , Delayed-Action Preparations , Female , Fibrin Tissue Adhesive , ... isothiocyanatostilbene-2,2'-disulfonic Acid / Wound Healing / Female / Humans / Male / Capillaries / Fibrin / Sodium ... Full text: Available Index: WPRIM (Western Pacific) Main subject: Rabbits / 4-Acetamido-4'- ... New Zealand white rabbits underwent intraperitoneal infection subsequent ileal anastomosis surgery were divided randomly into 4 ...
Disulfonic Acid, 4-Acetamido-4-isothiocyanatostilbene-2,2-disulfonic Acid, analogs & derivatives, pharmacology, Animals, ... Sensory neurons signal for an increase in rat gastric mucosal blood flow in the face of pending acid injury.. 1991 P Holzer 외 2 ... Activation of mast cells by bile acids.. 1991 R G Quist 외 4 명 Gastroenterology 118회 피인용 ... Mechanism of short-chain fatty acid uptake by apical membrane vesicles of rat distal colon.. 1991 N Mascolo 외 2 명 ...
Disulfonic Acid, 4-Acetamido-4-isothiocyanatostilbene-2,2-disulfonic Acid, analogs & derivatives, pharmacology, Animals, ... 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine, Animals, Cell Count, Cell Survival, drug effects, Mice, Mice, Inbred C57BL, ... start page previous page 1 2 3 4 5 next page end page ... 41 results for Journal of neuroscience research 30권 4호 * ...
... disulfonic acid (SITS, 0.5 mM), bumetanide (10 μM) or ouabain (1 mM). The cell shrinkage caused by a reduction of [Cl](o) from ... isothiocyanato-stilbene-2,2-disulfonic acid (SITS, 0.5 mM), bumetanide (10 μM) or ouabain (1 mM). The cell shrinkage caused by ... disulfonic acid (SITS, 0.5 mM), bumetanide (10 μM) or ouabain (1 mM). The cell shrinkage caused by a reduction of [Cl](o) from ... disulfonic acid (SITS, 0.5 mM), bumetanide (10 μM) or ouabain (1 mM). The cell shrinkage caused by a reduction of [Cl](o) from ...
  • A flickery, reversible block was produced by the diuretic stilbene 4-acetamido-4′-isothiocyanatostilbene-2,2′-disulfonic acid (SITS), while 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS) produced a permanent block. (deepdyve.com)
  • The binding of mannan to RBCs was demonstrated by flow cytometric analysis and was inhibited by the addition of band 3 protein inhibitor, 4,4′-diisothio-cyanatostilbene-2,2′-disulfonic acid (DIDS). (microbiologyresearch.org)
  • The haemolysis caused by mannan was inhibited by DIDS, SITS (4-acetamido-4′-isothiocyanatostilbene-2,2′-disulfonic acid) and bis(sulfosuccinimidyl) suberate, but not by pyridoxal 5-phosphate. (microbiologyresearch.org)
  • The stilbene derivative DIDS hyperpolarized the cell membrane potential from -57 +/- 2 to -71 +/- 4 mV and led to a significant increase of the K+-induced cell membrane depolarization, but prevented the K+-induced intracellular alkalinization. (nih.gov)
  • The stilbene DIDS (4,4-diisothiocyanatostilbenedisulphonic acid, 200 microM) also partially inhibited pHi recovery following an induced intracellular acidosis. (ox.ac.uk)
  • We conclude, that in the presence of CO2-HCO3-, a Na(+)- and HCO3-dependent (DIDS inhibitable) mechanism aids acid extrusion. (ox.ac.uk)
  • Sialic acid and sodium sulfate, which are transported only very slowly into the lumen of Golgi vesicles, are transported at low rates by the reconstituted proteoliposomes. (umassmed.edu)
  • 22Na+ uptake into confluent monolayers of cultured bovine corneal endothelial cells was studied in the presence of ouabain (10(-4)M) to inhibit active sodium extrusion. (mdc-berlin.de)
  • however, it was not attenuated by 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid (SITS), indicating the absence of SITS-sensitive HCO3(-)-dependent mechanisms. (ahajournals.org)
  • Admission hyperglycemia increased the risk of the occurrence of SICH by nearly 2-fold (adjusted RR: 1.891 [0.977-3.657], p = 0.0585 with the SITS-MOST criteria and adjusted RR: 1.884 [1.138-3.121], p = 0.0139 with the NINDS criteria). (elsevier.com)
  • In bicarbonate saline, uptake was reduced to a similar degree either by amiloride (10(-3)M) or by 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid (SITS) (10(-3)M). A further reduction was obtained with SITS-pretreated cells in the presence of amiloride. (mdc-berlin.de)
  • SITS-sensitive uptake was further characterized in saline containing both ouabain (10(-4)M) and amiloride (10(-3)M). It was absolutely dependent on bicarbonate, which could not be substituted by other plasma membrane permeable buffers (50 mM acetate or 25 mM glycodiazine). (mdc-berlin.de)
  • The primary sequence, composed of 527 amino acids, is identical in both the naturally occurring and synthetic proteases. (curehunter.com)
  • The extracellular domains (about 57 and 25 amino acids, respectively) share regions of sequence identity, but are quite variable in the position and number of amino acids with charged side chains, suggesting they might have different effects on the paracellular diffusion of ions. (jci.org)
  • Nucleic acid labeling compounds are disclosed. (google.com.au)
  • The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism. (google.com.au)
  • We investigated in a physiological salt solution (PSS) containing HCO3- the intracellular pH (pHi) regulating mechanisms in smooth muscle cells cultured from human internal mammary arteries, using the pH-sensitive dye 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) and 22Na+ influx rates. (ahajournals.org)
  • In the absence of HCO 3 - , intracellular alkalinization and acidification produced by NH 4 Cl exposure and withdrawal produced membrane hyperpolarization and depolarization, respectively, as expected for pH i -induced changes in g k+ . (elsevier.com)
  • Finally, continuous exposure to 10% CO 2 in the presence of HCO 3 - produced intracellular acidification, and the rate of pH i recovery from intracellular acidosis was inhibited by Ba 2+ , which blocks pH i -induced changes in g K+ , and by 4-acetamido-4′-isothiocyanatostilbene-2,2′-disulfonic acid, which inhibits Na + /HCO 3 - cotransport. (elsevier.com)
  • Mechanism of short-chain fatty acid uptake by apical membrane vesicles of rat distal colon. (naver.com)
  • The resulting proteoliposomes transport CMP-N-acetylneuraminic acid (CMP-AcNeu) and adenosine 3'-phosphate 5'-phosphosulfate with very similar affinity and inhibition characteristics as intact Golgi vesicles. (umassmed.edu)
  • Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon Patent Grant Nazarenko , et al. (uspto.report)
  • The optically recorded glomerular layer response was largely resistant to bath application of GABAA receptor antagonists but was sensitive to manipulations of external chloride concentration and to bath application of a stilbene derivative, 4- acetamido-4′isothiocyanatostilbene-2,2′-disulfonic acid known to block Cl- conductances. (jneurosci.org)
  • I Cl.ir was blocked by 9-anthracene-carboxylic acid and cadmium but not by stilbene disulfonates and tamoxifen. (ahajournals.org)
  • The systems and methods described herein relate to nucleic acid probes comprising a a pattern of universal and designate nucleotides, or 'gapped' probes, and the use of sets of gapped probes in sequencing by hybridization to determine the sequence of nucleic acid sequences. (google.com)
  • determining extension of the primer by detecting the unique signal from the labeled NTP to genotype or to sequence the target nucleic acid. (freepatentsonline.com)
  • Reverse transcription polymerase chain reaction and Northern blot analysis confirmed transcriptional expression of ClC-2 in both atrial and ventricular tissues and isolated myocytes of mouse and guinea pig hearts. (ahajournals.org)
  • A decrease in conductance correlated directly with the kinetics of claudin-4 induction. (jci.org)
  • 4. A method of claim 1 where the surface of the solid support comprises an array of locations each bearing a single molecule of polymerase or target DNA. (freepatentsonline.com)
  • 'Gastroenterology 101권 2호' 에 대한 전체 검색결과. (naver.com)
  • One hundred twenty-eight male and female New Zealand white rabbits underwent intraperitoneal infection subsequent ileal anastomosis surgery were divided randomly into 4 groups, including 32 animals in each, applied with saline solution, FG, rhVEGF165 and a mixture of rhVEGF165 with FG (VEGF + FG) on the anastomoses, respectively. (bvsalud.org)
  • To test whether members of the claudin family of tight junction proteins influence paracellular ionic selectivity, we expressed human claudin-4 in cultured MDCK cells using an inducible promoter. (jci.org)
  • 2. A method of claim 1 where the solution contains at least two different types of dNTP. (freepatentsonline.com)
  • 2. The method of claim 1, further comprising the step of linearizing said nucleic acid. (patentsencyclopedia.com)
  • 10. The method of claim 1, further comprising digesting the nucleic acid with one or more enzymes prior to the imaging step. (patentsencyclopedia.com)
  • The systems and methods described herein also relate to apparatus for sequencing nucleic acids which include gapped probes, as well as computer systems capable of analyzing data generated using gapped probes in such apparatus. (google.com)
  • The methods include comparing the restriction map of the nucleic acid with a restriction map database, and determining antibiotic resistance of the bacterium by matching regions of the nucleic acid to corresponding regions in the database. (patentsencyclopedia.com)
  • and(e) determining antibiotic resistance of the bacterium by matching regions of said nucleic acid to corresponding regions in said database. (patentsencyclopedia.com)
  • These results indicate that a novel I Cl.ir is present in mammalian heart and support a potentially important role of ClC-2 channels in the regulation of cardiac electrical activity and cell volume under physiological and pathological conditions. (ahajournals.org)
  • Recently, another member of the ClC Cl − channel family, ClC-2, has been cloned originally from rat heart and brain 18 and then from rabbit heart. (ahajournals.org)
  • M is a connecting group comprising a structure that does not interfere with the nucleic acid labeling compound's function. (google.com.au)
  • This invention relates to improved methods for sequencing and genotyping nucleic acid in a single molecule configuration. (freepatentsonline.com)
  • In the present study we directly test whether overexpression of one claudin, claudin-4, can confer ionic discrimination to the paracellular pathway. (jci.org)
  • The properties of I Cl.ir are consistent with currents generated by expression of ClC-2 Cl − channels. (ahajournals.org)
  • The findings that [14C] acetate exhibits a much lower capacity for cell entry and that the structural analog alpha-cyano-3-hydroxycinnamic acid inhibits pyruvate release provide additional support for the presence of a pyruvate transporter. (nih.gov)
  • By contrast, in the presence of HCO 3 - , NH 4 Cl exposure and withdrawal produced a decrease in apparent buffering capacity and changes in membrane potential difference consistent with compensatory regulation of electrogenic Na + /HCO 3 - cotransport. (elsevier.com)
  • Sensory neurons signal for an increase in rat gastric mucosal blood flow in the face of pending acid injury. (naver.com)
  • In proximal tubules an increase of the K+ concentration in the peritubular perfusate from 3 to 15 mmol/liter decreased the peritubular cell membrane potential from -55 +/- 2 to -38 +/- 1 mV paralleled by an increase of pHi from 7.54 +/- 0.02 to 7.66 +/- 0.02. (nih.gov)
  • Activation of mast cells by bile acids. (naver.com)
  • 2. Steady-state pHi in cells bathed in a HEPES-buffered Tyrode solution (pH 7.4) was found to be remarkably alkaline (pHi = 7.77) whereas cells bathed in a CO2-HCO3(-)-buffered Tyrode solution (pH 7.4) had a more 'normal' pHi (pHi = 7.28). (ox.ac.uk)
  • However, hyperpolarization-activated ClC-2 channels expressed in heterologous expression systems bear a striking resemblance to the well-characterized hyperpolarization-activated cationic pacemaker current, I f (or I h ), found in many mammalian cardiac cell types 21 22 and are now known to belong to the HCN gene family. (ahajournals.org)