4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid: A non-penetrating amino reagent (commonly called SITS) which acts as an inhibitor of anion transport in erythrocytes and other cells.4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid: An inhibitor of anion conductance including band 3-mediated anion transport.Naphthalenesulfonates: A class of organic compounds that contains a naphthalene moiety linked to a sulfonic acid salt or ester.Receptors, Purinergic P2X: A subclass of purinergic P2 receptors that signal by means of a ligand-gated ion channel. They are comprised of three P2X subunits which can be identical (homotrimeric form) or dissimilar (heterotrimeric form).Purinergic P2 Receptor Antagonists: Compounds that bind to and block the stimulation of PURINERGIC P2 RECEPTORS.Pyridoxal Phosphate: This is the active form of VITAMIN B 6 serving as a coenzyme for synthesis of amino acids, neurotransmitters (serotonin, norepinephrine), sphingolipids, aminolevulinic acid. During transamination of amino acids, pyridoxal phosphate is transiently converted into pyridoxamine phosphate (PYRIDOXAMINE).Receptors, Purinergic P2: A class of cell surface receptors for PURINES that prefer ATP or ADP over ADENOSINE. P2 purinergic receptors are widespread in the periphery and in the central and peripheral nervous system.Suramin: A polyanionic compound with an unknown mechanism of action. It is used parenterally in the treatment of African trypanosomiasis and it has been used clinically with diethylcarbamazine to kill the adult Onchocerca. (From AMA Drug Evaluations Annual, 1992, p1643) It has also been shown to have potent antineoplastic properties.1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt: A colorimetric reagent for iron, manganese, titanium, molybdenum, and complexes of zirconium. (From Merck Index, 11th ed)Cephalosporins: A group of broad-spectrum antibiotics first isolated from the Mediterranean fungus ACREMONIUM. They contain the beta-lactam moiety thia-azabicyclo-octenecarboxylic acid also called 7-aminocephalosporanic acid.Stilbenes: Organic compounds that contain 1,2-diphenylethylene as a functional group.Chlorides: Inorganic compounds derived from hydrochloric acid that contain the Cl- ion.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Adenosine Triphosphate: An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.Cefotiam: One of the CEPHALOSPORINS that has a broad spectrum of activity against both gram-positive and gram-negative microorganisms.Structure-Activity Relationship: The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.Chemistry: A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.Chemical Phenomena: The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Bicarbonates: Inorganic salts that contain the -HCO3 radical. They are an important factor in determining the pH of the blood and the concentration of bicarbonate ions is regulated by the kidney. Levels in the blood are an index of the alkali reserve or buffering capacity.Acetamides: Derivatives of acetamide that are used as solvents, as mild irritants, and in organic synthesis.Magnetic Resonance Spectroscopy: Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).Acetylgalactosamine: The N-acetyl derivative of galactosamine.Spectrophotometry, Infrared: Spectrophotometry in the infrared region, usually for the purpose of chemical analysis through measurement of absorption spectra associated with rotational and vibrational energy levels of molecules. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Acetylglucosamine: The N-acetyl derivative of glucosamine.Antiporters: Membrane transporters that co-transport two or more dissimilar molecules in the opposite direction across a membrane. Usually the transport of one ion or molecule is against its electrochemical gradient and is "powered" by the movement of another ion or molecule with its electrochemical gradient.Carbohydrate Sequence: The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.Carbohydrate Conformation: The characteristic 3-dimensional shape of a carbohydrate.Plant Lectins: Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.Microbial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.Oligosaccharides: Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.Administration, Oral: The giving of drugs, chemicals, or other substances by mouth.Membrane Potentials: The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).Lectins: Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.Kinetics: The rate dynamics in chemical or physical systems.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Molecular Structure: The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Nucleic Acid Probes: Nucleic acid which complements a specific mRNA or DNA molecule, or fragment thereof; used for hybridization studies in order to identify microorganisms and for genetic studies.Peptide Nucleic Acids: DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.DNA Probes: Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.Tissue Plasminogen Activator: A proteolytic enzyme in the serine protease family found in many tissues which converts PLASMINOGEN to FIBRINOLYSIN. It has fibrin-binding activity and is immunologically different from UROKINASE-TYPE PLASMINOGEN ACTIVATOR. The primary sequence, composed of 527 amino acids, is identical in both the naturally occurring and synthetic proteases.Fibrinolysin: A product of the lysis of plasminogen (profibrinolysin) by PLASMINOGEN activators. It is composed of two polypeptide chains, light (B) and heavy (A), with a molecular weight of 75,000. It is the major proteolytic enzyme involved in blood clot retraction or the lysis of fibrin and quickly inactivated by antiplasmins.Urokinase-Type Plasminogen Activator: A proteolytic enzyme that converts PLASMINOGEN to FIBRINOLYSIN where the preferential cleavage is between ARGININE and VALINE. It was isolated originally from human URINE, but is found in most tissues of most VERTEBRATES.Plasminogen: Precursor of plasmin (FIBRINOLYSIN). It is a single-chain beta-globulin of molecular weight 80-90,000 found mostly in association with fibrinogen in plasma; plasminogen activators change it to fibrinolysin. It is used in wound debriding and has been investigated as a thrombolytic agent.Gabexate: A serine proteinase inhibitor used therapeutically in the treatment of pancreatitis, disseminated intravascular coagulation (DIC), and as a regional anticoagulant for hemodialysis. The drug inhibits the hydrolytic effects of thrombin, plasmin, and kallikrein, but not of chymotrypsin and aprotinin.Plasminogen Activator Inhibitor 1: A member of the serpin family of proteins. It inhibits both the tissue-type and urokinase-type plasminogen activators.Plasminogen Activators: A heterogeneous group of proteolytic enzymes that convert PLASMINOGEN to FIBRINOLYSIN. They are concentrated in the lysosomes of most cells and in the vascular endothelium, particularly in the vessels of the microcirculation.
List of MeSH codes (D02)... disulfonic acid MeSH D02.455.426.559.389.150.700.550 --- stilbamidines MeSH D02.455.426.559.389.150.700.900 --- tamoxifen MeSH ... isothiocyanatostilbene-2,2'-disulfonic acid MeSH D02.455.426.559.389.150.700.075 --- bibenzyls MeSH D02.455.426.559.389.150. ... edetic acid MeSH D02.241.081.038.455 --- egtazic acid MeSH D02.241.081.038.581 --- iodoacetic acid MeSH D02.241.081.038.581.400 ... muramic acids MeSH D02.241.081.844.562 --- neuraminic acids MeSH D02.241.081.844.562.668 --- sialic acids MeSH D02.241.081.844. ...
4,4'-Diisothiocyano-2,2'-stilbenedisulfonic acid (DIDS) is an anion exchange inhibitor, blocking reversibly, and later irreversibly, exchangers such as chloride-bicarbonate exchanger. Jessen, Flemming; Sjøholm, C; Hoffmann, EK (1986), "Identification of the anion exchange protein of ehrlich cells: A kinetic analysis of the inhibitory effects of 4,4′-diisothiocyano-2,2′-stilbene-disulfonic acid (DIDS) and labeling of membrane proteins with3H-DIDS", Journal of Membrane Biology, 92 (3): 195, doi:10.1007/BF01869388, PMID 3783658 Lane, Michelle; Baltz, Jay M.; Bavister, Barry D. (1999), "Bicarbonate/Chloride Exchange Regulates Intracellular pH of Embryos but Not Oocytes of the Hamster", Biology of Reproduction, 61 (2): 452-457, doi:10.1095/biolreprod61.2.452, PMID 10411526 ...
... (EC 2.3.1.201, WbpD, WlbB) is an enzyme with systematic name acetyl-CoA:UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate N-acetyltransferase. This enzyme catalyses the following chemical reaction acetyl-CoA + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate ⇌ {\displaystyle \rightleftharpoons } CoA + UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate This enzyme participates in the biosynthetic pathway for UDP-alpha-D-ManNAc3NAcA (UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-mannuronic acid). Westman, E.L.; McNally, D.J.; Charchoglyan, A.; Brewer, D.; Field, R.A.; Lam, J.S. (2009). "Characterization of WbpB, WbpE, and WbpD and reconstitution of a pathway for the biosynthesis of UDP-2,3-diacetamido-2,3-dideoxy-D-mannuronic acid in Pseudomonas aeruginosa". J. Biol. Chem. 284: 11854-11862. doi:10.1074/jbc.M808583200. PMC 2673254 . PMID 19282284. Larkin, A.; Imperiali, B. (2009). ...
4,4'-Diisothiocyano-2,2'-stilbenedisulfonic acid (DIDS) is an anion exchange inhibitor, blocking reversibly, and later irreversibly, exchangers such as chloride-bicarbonate exchanger. Jessen, Flemming; Sjøholm, C; Hoffmann, EK (1986), "Identification of the anion exchange protein of ehrlich cells: A kinetic analysis of the inhibitory effects of 4,4′-diisothiocyano-2,2′-stilbene-disulfonic acid (DIDS) and labeling of membrane proteins with3H-DIDS", Journal of Membrane Biology, 92 (3): 195, doi:10.1007/BF01869388, PMID 3783658 Lane, Michelle; Baltz, Jay M.; Bavister, Barry D. (1999), "Bicarbonate/Chloride Exchange Regulates Intracellular pH of Embryos but Not Oocytes of the Hamster", Biology of Reproduction, 61 (2): 452-457, doi:10.1095/biolreprod61.2.452, PMID 10411526 ...
Cos allargat i recobert d'escates. Línia lateral única i situada al llarg de tot el cos. Boca terminal i protràctil. 1 única i allargada aleta dorsal, la qual té 4-7 espines curtes i 19-27 radis tous. Aleta anal més curta que la dorsal, amb 1-2 espines i 17-25 radis tous. Aletes pelvianes amb 1 espina i 5 radis tous. Aleta caudal de truncada a força semilunar i amb 13-15 radis ramificats. La inserció de les aletes pelvianes es troba per sota o una mica abans de les pectorals.[5][6] ...
... (or signaling: see American and British English differences) is a form of extracellular signalling mediated by purine nucleotides and nucleosides such as adenosine and ATP. It involves the activation of purinergic receptors in the cell and/or in nearby cells, thereby regulating cellular functions. The purinergic signalling complex of a cell is sometimes referred to as the "purinome". Purinergic receptors, represented by several families, are among the most abundant receptors in living organisms and appeared early in evolution. Among invertebrates, the purinergic signalling system has been found in bacteria, amoeba, ciliates, algae, fungi, anemones, ctenophores, platyhelminthes, nematodes, crustacea, molluscs, annelids, echinoderms, and insects. In green plants, extracellular ATP and other nucleotides induce an increase in the cytosolic concentration of calcium ions, in addition to other downstream changes that influence plant growth and modulate responses to stimuli. In ...
Adrian K, Bernhard MK, Breitinger HG, Ogilvie A (Sep 2000). "Expression of purinergic receptors (ionotropic P2X1-7 and metabotropic P2Y1-11) during myeloid differentiation of HL60 cells". Biochim Biophys Acta 1492 (1): 127-38. PMID 11004484. Cite uses deprecated parameter ...
... , often abbreviated AOA or AOAA, is a compound that inhibits 4-aminobutyrate aminotransferase (GABA-T) activity in vitro and in vivo, leading to less gamma-aminobutyric acid (GABA) being broken down. Subsequently, the level of GABA is increased in tissues. At concentrations high enough to fully inhibit 4-aminobutyrate aminotransferase activity, aminooxyacetic acid is indicated as a useful tool to study regional GABA turnover in rats. Aminooxyacetic acid is a general inhibitor of pyridoxal phosphate (PLP)-dependent enzymes (this includes GABA-T). It functions as an inhibitor by attacking the Schiff base linkage between PLP and the enzyme, forming oxime type complexes. Aminooxyacetic acid inhibits aspartate aminotransferase, another PLP-dependent enzyme, which is an essential part of the malate-aspartate shuttle. The inhibition of the malate-aspartate shuttle prevents the reoxidation of cytosolic NADH by the mitochondria in nerve ...
Two enzymes convert L-amino acids to D-amino acids. D-Amino-acid racemase, a PLP-dependent enzyme, racemizes amino acids via the formation of the alpha-iminoacids, where the stereogenic center is lost. L-amino-acid oxidases convert L-amino acids to the alpha-ketoacids, which are susceptible to reductive amination. Some amino acids are prone to racemization, one example being lysine, which racemizes via formation pipecolic acid. In peptides, L-amino acid residues slowly racemize, resulting in the formation of some D-amino acid residues. Racemization occurs via deprotonation of the methyne that is alpha to the amido group. Rates increase with pH. Many D-amino acids found in higher organisms are derived from microbial sources. The D-alanine in peptidoglycans that comprise bacterial cell walls ...
... or tryptophan synthetase is an enzyme that catalyzes the final two steps in the biosynthesis of tryptophan. It is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae. However, it is absent from Animalia. It is typically found as an α2β2 tetramer. The α subunits catalyze the reversible formation of indole and glyceraldehyde-3-phosphate (G3P) from indole-3-glycerol phosphate (IGP). The β subunits catalyze the irreversible condensation of indole and serine to form tryptophan in a pyridoxal phosphate (PLP) dependent reaction. Each α active site is connected to a β active site by a 25 angstrom long hydrophobic channel contained within the enzyme. This facilitates the diffusion of indole formed at α active sites directly to β active sites in a process known as substrate channeling. The active sites of tryptophan synthase are allosterically coupled. Subunits: Tryptophan synthase typically exists as an α-ββ-α complex. The α and β subunits have ...
... catalyzed by aminotransferase occurs in two stages. In the first step, the α amino group of an aminoacid is transferred to the enzyme, producing the corresponding α-keto acid and the aminated enzyme. During the second stage, the amino group is transferred to the keto acid acceptor, forming the amino acid product while regenerating the enzyme. The chirality of an amino acid is determined during transamination. For the reaction to complete, aminotransferases require participation of aldehyde containing coenzyme, pyridoxal-5'-phosphate (PLP), a derivative of Pyridoxine (Vitamin B6). The amino group is accommodated by conversion of this coenzyme to pyridoxamine-5'-phosphate (PMP). PLP is covalently attached to the enzyme via a Schiff Base linkage formed by the condensation of its aldehyde group with the ε-amino group of an enzymatic Lys residue. The Schiff base, which is conjugated to the enzymes pyridinium ring is the ...
GAD67 and GAD65 are also regulated differently post-translationally. Both GAD65 and GAD67 are regulated via phosphorylation of a dynamic catalytic loop,[10][11] but the regulation of these isoforms differs; GAD65 is activated by phosphorylation while GAD67 is inhibited by phosphorylation. GAD67 is predominantly found activated (~92%), whereas GAD65 is predominantly found inactivated (~72%).[12] GAD67 is phosphorylated at threonine 91 by protein kinase A (PKA), while GAD65 is phosphorylated, and therefore regulated by, protein kinase C (PKC). Both GAD67 and GAD65 are also regulated post-translationally by Pyridoxal 5'-phosphate (PLP); GAD is activated when bound to PLP and inactive when not bound to PLP.[12] Majority of GAD67 is bound to PLP at any given time, whereas GAD65 binds PLP when GABA is needed for neurotransmission.[12] This reflects the functional properties of the two isoforms; GAD67 must be active at all times for normal cellular functioning, and is therefore constantly activated by ...
Cette enzyme utilise le phosphate de pyridoxal comme cofacteur[2]. Chez les archées et les eucaryotes, la formation du sélénocystéinyl-ARNtSec se déroule en effet en deux étapes[3],[4],[5] : la O-phosphoséryl-ARNtSec kinase catalyse tout d'abord la phosphorylation d'un L-séryl-ARNtSec en O-phosphoL-séryl-ARNtSec, lequel est ensuite converti en L-sélénocystéinyl-ARNtSec par la O-phosphoséryl-ARNt:sélénocystéinyl-ARNt synthase. ...
在人体中，组氨酸脱羧酶由HDC基因编码。[4][5]. 生物胺组织胺在许多生理过程中有重要的调节作用，包括神经传递、胃酸分泌、平滑肌舒张。从组氨酸到组织胺的生物合成由L-组氨酸脱羧酶催化。这个同源二聚体酶是一个依赖磷酸吡哆醛（PLP）的脱羧酶，并对其底物组氨酸有高度特异性。[4]. ...
Patent US6689563 - System and methods for sequencing by hybridization - Google PatentsThe systems and methods described herein also relate to apparatus for sequencing nucleic acids which include gapped probes, as ... and the use of sets of gapped probes in sequencing by hybridization to determine the sequence of nucleic acid sequences. The ... The systems and methods described herein relate to nucleic acid probes comprising a a pattern of universal and designate ... disulfonic acid; pyrene-3-sulfonic acid; 2-toluidinonaphthalene-6-sulfonate; N-phenyl-N-methyl-2-aminoaphthalene-6-sulfonate; ...
Tissue Plasminogen Activator
- Alteplase
Summary Report | CureHunterThe primary sequence, composed of 527 amino acids, is identical in both the naturally occurring and synthetic proteases. ... disulfonic Acid (SITS) Related Therapies and Procedures. 1. Thrombolytic Therapy 2. Catheters ... Acetamido- 4- isothiocyanatostilbene- 2,2- ... The primary sequence, composed of 527 amino acids, is identical ... Amino Acids, Peptides, and Proteins*Proteins: 90489*Blood Proteins: 4303*Blood Coagulation Factors: 2147*Plasminogen Activators ...
... disulfonic acid, 4-acetamido-4-isothiocyanato-stilbene-2,2-disulfonic acid, 4,4-diisothiocyanatostilbene-2,2-disulfonic ... disulfonic acid4-acetamido-4-isothiocyanato- 336 438 Mstilbene-2,2-disulfonic acid4,4-Diisothiocyanatostilbene 342 419 M2,2 ... Target nucleic acid sequences to be hybridized in the present methods can be present in any nucleic acid-containing sample so ... For instance, nucleic acids can be isolated from a variety of nucleic acid-containing samples including body tissue, such as ...
Plus it... disulfonic acid known to block Cl- conductances. It is suggested the the voltage-sensitive dye signals recorded from the ... acetamido-4′isothiocyanatostilbene-2,2′- ...
Intracellular pH in human arterial smooth muscle. Regulation by Na+/H+ exchange and a novel 5-(N-ethyl-N-isopropyl)amiloride...... disulfonic acid (SITS), indicating the absence of SITS-sensitive HCO3(-)-dependent mechanisms. Recovery instead appeared mostly ... isothiocyanatostilbene-2,2- ... using the pH-sensitive dye 2,7-bis(2-carboxyethyl)-5(6)- ... Recovery of pHi was dependent on extracellular Na+ (Km, 13.1 mM); however, it was not attenuated by 4-acetamido-4- ...
The channel is blocked by carboxylic acid derivatives and Cd2+ but is largely unaffected by disulfonic stilbene derivatives and ... isothiocyanatostilbene-2,2′-disulfonic acid (SITS) have been identified as effective blockers of a variety of cardiac5 38 39 ... Several compounds including arylaminoalkyl benzoates derivatives such as 9-anthracene-carboxylic acid (9-AC) and disulfonic ... A, Carboxylic acid derivative 9-AC blocked ICl.ir in mouse atrial myocytes. A-a, Mean I-V curves of ICl.ir obtained from 4 ...
A Large-Conductance Chloride Channel in Pigmented Ciliary Epithelial Cells Activated by GTPγS, The Journal of Membrane Biology ...... disulfonic acid (DIDS) produced a permanent block. The channel was rarely active in cell-attached patches and usually required ... isothiocyanatostilbene-2,2′-disulfonic acid (SITS), while 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS) produced a ... Add Journal to My Library The Journal of Membrane Biology , Volume 158 (2) - Jul 15, 1997 ... A flickery, reversible block was produced by the diuretic stilbene 4-acetamido-4′-isothiocyanatostilbene-2,2′-disulfonic acid ( ...
Microbiology Society Journals | Characterization of a haemolytic factor from Candida albicans... disulfonic acid (DIDS). The haemolysis caused by mannan was inhibited by DIDS, SITS (4-acetamido-4′-isothiocyanatostilbene-2,2 ... disulfonic acid) and bis(sulfosuccinimidyl) suberate, but not by pyridoxal 5-phosphate. These results indicated that a ... isothiocyanatostilbene-2,2′-disulfonic acid) and bis(sulfosuccinimidyl) suberate, but not by pyridoxal 5-phosphate. These ... disulfonic acid (DIDS). The haemolysis caused by mannan was inhibited by DIDS, SITS (4-acetamido-4′- ...
DISULFONIC ACID. DOM. 2,5-DIMETHOXY-4-METHYLAMPHETAMINE. EIMERIINA. EIMERIIDA. EPN. PHENYLPHOSPHONOTHIOIC ACID, 2-ETHYL 2-(4- ... ISOTHIOCYANATOSTILBENE-2,2-DISULFONIC ACID. SPOROZOEA. APICOMPLEXA. SPOROZOEA INFECTIONS. PROTOZOAN INFECTIONS. TYPHOID. ...
Sustained-released mixture of vascular endothelial growth factor 165 and fibrin glue strengthens healing of ileal anastomoses...... disulfonic Acid , Anastomotic Leak , Animals , Capillaries , Delayed-Action Preparations , Female , Fibrin Tissue Adhesive , ... isothiocyanatostilbene-2,2'-disulfonic Acid / Wound Healing / Female / Humans / Male / Capillaries / Fibrin / Sodium ... Full text: Available Index: WPRIM (Western Pacific) Main subject: Rabbits / 4-Acetamido-4'- ... New Zealand white rabbits underwent intraperitoneal infection subsequent ileal anastomosis surgery were divided randomly into 4 ...
NAVER 학술정보 >...Disulfonic Acid, 4-Acetamido-4-isothiocyanatostilbene-2,2-disulfonic Acid, analogs & derivatives, pharmacology, Animals, ... Sensory neurons signal for an increase in rat gastric mucosal blood flow in the face of pending acid injury.. 1991 P Holzer 외 2 ... Activation of mast cells by bile acids.. 1991 R G Quist 외 4 명 Gastroenterology 118회 피인용 ... Mechanism of short-chain fatty acid uptake by apical membrane vesicles of rat distal colon.. 1991 N Mascolo 외 2 명 ...
Disulfonic Acid, 4-Acetamido-4-isothiocyanatostilbene-2,2-disulfonic Acid, analogs & derivatives, pharmacology, Animals, ... 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine, Animals, Cell Count, Cell Survival, drug effects, Mice, Mice, Inbred C57BL, ... start page previous page 1 2 3 4 5 next page end page ... 41 results for Journal of neuroscience research 30권 4호 * ...
Disulfonic Acid, 4-Acetamido-4-isothiocyanatostilbene-2,2-disulfonic Acid, analogs & derivatives, pharmacology, Anion ... Formation of alpha-tocopherol complexes with fatty acids. Nature of complexes.. 1985 A N Erin et al. Biochimica et biophysica ... Fatty Acids, Hydrogen Bonding, Magnetic Resonance Spectroscopy, Models, Molecular, Spectrophotometry, Ultraviolet, Temperature ... Adaptation, Physiological, Calorimetry, Cell Membrane, physiology, Fatty Acids, Fluorescence Polarization, Geobacillus ...
Fused cells of frog proximal tubule: II. Voltage-dependent intracellular pH. - PubMed - NCBI... disulfonic Acid/analogs & derivatives. *4-Acetamido-4-isothiocyanatostilbene-2,2-disulfonic Acid/pharmacology ... isothiocyanatostilbene-2,2- ... 2 to -71 +/- 4 mV and led to a significant increase of the K+- ... 2 to -38 +/- 1 mV paralleled by an increase of pHi from 7.54 +/- 0.02 to 7.66 +/- 0.02. The stilbene derivative DIDS ...
... disulfonic Acid/analogs & derivatives. *4-Acetamido-4-isothiocyanatostilbene-2,2-disulfonic Acid/pharmacology ... isothiocyanatostilbene-2,2- ... the specific band 3 anion channel blocking agents niflumic acid ... molecules.mu-2.s-1 was calculated. The hypoxia induced ATP release was blocked in three different ways: first, by application ...
... disulfonic acid, which inhibits Na+/HCO3- cotransport. These findings suggest that in hepatocytes, changes in transmembrane ... isothiocyanatostilbene-2,2′-disulfonic acid, which inhibits Na+/HCO3- cotransport. These findings suggest that in hepatocytes, ... disulfonic acid, which inhibits Na+/HCO3- cotransport. These findings suggest that in hepatocytes, changes in transmembrane ... disulfonic acid, which inhibits Na+/HCO3- cotransport. These findings suggest that in hepatocytes, changes in transmembrane ...
The regulated release of transmitters from astrocytes | Springer for Research & Development... disulfonic acid. SD. Spreading depression. SITS. 4-acetamido-4′-isothiocyanato-stilbene-2,2′-disulfonate ... Seal RP, Amara SG (1999) Excitatory amino acid transporters: a family in flux. Annu Rev Pharmacol Toxicol 39:431-456.PubMed ... Maycox PR, Hell JW, Jahn R (1990) Amino acid neurotransmission: spotlight on synaptic vesicles. Trends Neurosci 13:83-87.PubMed ... Duan S, Anderson CM, Keung EC, Chen Y, Swanson RA (2003) P2X7 receptor-mediated release of excitatory amino acids from ...
The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a ... Nucleic acid labeling compounds are disclosed. The compounds are synthesized by condensing a heterocyclic derivative with a ... disulfonic acid; pyrene-3-sulfonic acid; 2-toluidinonapththalene-6-sulfonate; N-phenyl, N-methyl 2-aminonaphthalene-6-sulfonate ... attaching at least one nucleic acid to a support; and. coupling the at least one nucleic acid to the nucleic acid labeling ...
... disulfonic acid; pyrene-3-sulfonic acid; 2-toluidinonaphthalene-6-sulfonate; N-phenyl-N-methyl-2-aminoaphthalene-6-sulfonate; ... Method for analyzing nucleic acids by means of a substrate having a microchannel structure containing immobilized nucleic acid ... Integrated nucleic acid diagnostic device. US6213151. 25 Apr 2000. 10 Apr 2001. Ut-Battelle, Llc. Microfluidic circuit designs ... In some cases, the amino acid tryptophan, which is either part of a peptide or protein of interest (i.e., it is endogenous to ...
... disulfonic Acid, Bumetanide, Calcium, Chloride Channels, Chlorides, Culture Media, Cytosol, Epithelium, Humans, Intestine, ... isothiocyanatostilbene-2,2- ... disulphonic acid) also abolished the cell-swelling activated ... disulphonic acid) also abolished the cell-swelling activated increase in SCC; DIDS is without effect upon the VIP-stimulated ... disulphonic acid) also abolished the cell-swelling activated increase in SCC; DIDS is without effect upon the VIP-stimulated ...
... disulfonic Acid , Anastomotic Leak , Animals , Capillaries , Delayed-Action Preparations , Female , Fibrin Tissue Adhesive , ... isothiocyanatostilbene-2,2'- ... serum uric acid,FBG,FINS,hs-CRP,ISI and HOMA-IR between the two ... Objective To compare the effects of losartan and irbesartan on blood pressure,blood uric acid,insulin sensitivity and insulin ... the serum uric acid in the observation group was lower than that in the control group [(387.21 ± 25.56) μmol/L vs.(429.67 ± ...
Toronto Research Chemicals | Page 6... disulfonic Acid, Sodium Salt CAS number: 51023-76-8 Alternate CAS: Free Acid: 27816-59-7 ... O-(5-Acetamido-4,7,8,9-tetra-O-acetyl-3-thiophenyl-α-neuraminosyl Methyl Ester)-2-α-3-O-(6-O-benzyl-β-D-galactopyranosyl)-1-β-4 ... isothiocyanatostilbene-2,2- ... Acexamic Acid; CY 153; N-Acetyl-6-aminohexanoic Acid; AACA; N- ... 6-(Acetylamino)hexanoic Acid; 6-Acetamidocaproic Acid; Acemin; Acetaminocaproic Acid; ...
Disulfonic Acid. *4-Acetamido-4-isothiocyanatostilbene-2,2-disulfonic Acid. *5-Hydroxytryptophan ...
METHODS OF DETERMINING ANTIBIOTIC RESISTANCE - Patent application... disulfonic acid; 4,4-diisothiocyanatostilbene-2,2-disulfonic acid; 5-[dimethylamino]naphthalene-1-sulfonyl chloride (DNS, ... isothiocyanatostilbene-2,2disulfonic acid; acridine and derivatives: acridine, acridine isothiocyanate; 5-(2-aminoethyl) ... 8. The method of claim 1, wherein the nucleic acid is deoxyribonucleic acid. 9. The method of claim 1, wherein the nucleic acid ... 0011]The nucleic acid can be deoxyribonucleic acid (DNA), a ribonucleic acid (RNA) or can be a cDNA copy of an RNA obtained ...
System and method for nucleic acid sequencing by polymerase synthesis - LI-COR, Inc.This invention relates to improved methods for sequencing and genotyping nucleic acid in a single molecule configuration. The ... disulfonic acid; acridine and derivatives: acridine, acridine isothiocyanate; 5-(2′-aminoethyl)aminonaphthalene-1-sulfonic acid ... isothiocyanatostilbene-2,2′ ... Sources of target nucleic acid. [0030] The target nucleic acid ... Nucleic Acids Research, 15: 5305-5321 (1987) (3′ thiol group on oligonucleotide); Sharma et al., Nucleic Acids Research, 19: ...
- A flickery, reversible block was produced by the diuretic stilbene 4-acetamido-4′-isothiocyanatostilbene-2,2′-disulfonic acid (SITS), while 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS) produced a permanent block. (deepdyve.com)
- The binding of mannan to RBCs was demonstrated by flow cytometric analysis and was inhibited by the addition of band 3 protein inhibitor, 4,4′-diisothio-cyanatostilbene-2,2′-disulfonic acid (DIDS). (microbiologyresearch.org)
- The haemolysis caused by mannan was inhibited by DIDS, SITS (4-acetamido-4′-isothiocyanatostilbene-2,2′-disulfonic acid) and bis(sulfosuccinimidyl) suberate, but not by pyridoxal 5-phosphate. (microbiologyresearch.org)
- The stilbene derivative DIDS hyperpolarized the cell membrane potential from -57 +/- 2 to -71 +/- 4 mV and led to a significant increase of the K+-induced cell membrane depolarization, but prevented the K+-induced intracellular alkalinization. (nih.gov)
- The optically recorded glomerular layer response was largely resistant to bath application of GABAA receptor antagonists but was sensitive to manipulations of external chloride concentration and to bath application of a stilbene derivative, 4- acetamido-4′isothiocyanatostilbene-2,2′-disulfonic acid known to block Cl- conductances. (jneurosci.org)
- I Cl.ir was blocked by 9-anthracene-carboxylic acid and cadmium but not by stilbene disulfonates and tamoxifen. (ahajournals.org)
- Formation of alpha-tocopherol complexes with fatty acids. (naver.com)
- Resistance of tumor microsomes to lipid peroxidation is associated with the low content of substrates in the form of polyunsaturated fatty acids (PUFAs), higher level of alpha-tocopherol, reduced glutathione and protein thiols and altered enzymic antioxidant potential (catalase and superoxide dismutase). (bvsalud.org)
- The effects of changes in fatty acid composition of the cell membrane on different biological functions of Salmonella typhimurium have been studied with the help of a temperature sensitive fatty acid auxotroph which cannot synthesise unsaturated fatty acids at high temperature. (bvsalud.org)
- determining extension of the primer by detecting the unique signal from the labeled NTP to genotype or to sequence the target nucleic acid. (freepatentsonline.com)
- 12. A method claim 1 wherein the target nucleic acid is deoxynucleotide acid, the polymerase is either a DNA dependent DNA polymerase or a RNA dependent DNA polymerase and the NTPs are dNTPs. (freepatentsonline.com)
- 13. A method of claim 1 wherein the target nucleic acid is double stranded DNA and the polymerase is RNA polymerase and the NTPs are rNTPs. (freepatentsonline.com)
- The primary sequence, composed of 527 amino acids, is identical in both the naturally occurring and synthetic proteases. (curehunter.com)
- Biochimica et Biophysica Acta , 1135 (2), 180-3. (elsevier.com)
- Nucleic acid labeling compounds are disclosed. (google.com.au)
- The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism. (google.com.au)
- In proximal tubules an increase of the K+ concentration in the peritubular perfusate from 3 to 15 mmol/liter decreased the peritubular cell membrane potential from -55 +/- 2 to -38 +/- 1 mV paralleled by an increase of pHi from 7.54 +/- 0.02 to 7.66 +/- 0.02. (nih.gov)
- however, it was not attenuated by 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid (SITS), indicating the absence of SITS-sensitive HCO3(-)-dependent mechanisms. (ahajournals.org)
- Mechanism of short-chain fatty acid uptake by apical membrane vesicles of rat distal colon. (naver.com)
- Groups P, PP, and C underwent anastomosis of the distal colon, and subgroups (n = 4) of each were sacrificed on postoperative day 3, 7, 14 and 21. (bvsalud.org)
- 12. The method of claim 11 wherein each R 1 , R 2 , and R 3 is phenyl and R 4 is butyl, or benzyl. (patentsencyclopedia.com)
- METHODS: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were performed to investigate the effect of PAB treatment in various cervical cancer cell lines. (bvsalud.org)
- 1992 Jun 10;1135(2):180-3. (elsevier.com)
- 4. The method according to claim 3, wherein the S. aureus is a community-acquired methicillin-resistant strain of S. aureus. (patentsencyclopedia.com)
- The methods include comparing the restriction map of the nucleic acid with a restriction map database, and determining antibiotic resistance of the bacterium by matching regions of the nucleic acid to corresponding regions in the database. (patentsencyclopedia.com)
- and(e) determining antibiotic resistance of the bacterium by matching regions of said nucleic acid to corresponding regions in said database. (patentsencyclopedia.com)
- Reverse transcription polymerase chain reaction and Northern blot analysis confirmed transcriptional expression of ClC-2 in both atrial and ventricular tissues and isolated myocytes of mouse and guinea pig hearts. (ahajournals.org)
- In group 2, colon anastomosis then hyperthermic oxaliplatin perfusion was performed. (bvsalud.org)
- This invention relates to improved methods for sequencing and genotyping nucleic acid in a single molecule configuration. (freepatentsonline.com)
- The invention generally relates to methods for detecting fetal nucleic acids and methods for diagnosing fetal abnormalities. (patentsencyclopedia.com)
- In certain embodiments, the invention provides methods for determining whether fetal nucleic acid is present in a maternal sample including obtaining a maternal sample suspected to include fetal nucleic acids, and performing a sequencing reaction on the sample to determine presence of at least a portion of a Y chromosome in the sample, thereby determining that fetal nucleic acid is present in the sample. (patentsencyclopedia.com)
- In other embodiments, the invention provides methods for quantitative or qualitative analysis to detect fetal nucleic acid in a maternal sample, regardless of the ability to detect the Y chromosome, particularly for samples including normal nucleic acids from a female fetus. (patentsencyclopedia.com)
- In the absence of HCO 3 - , intracellular alkalinization and acidification produced by NH 4 Cl exposure and withdrawal produced membrane hyperpolarization and depolarization, respectively, as expected for pH i -induced changes in g k+ . (elsevier.com)
- The systems and methods described herein also relate to apparatus for sequencing nucleic acids which include gapped probes, as well as computer systems capable of analyzing data generated using gapped probes in such apparatus. (google.com)
- M is -CH 2 -NR 3 -, wherein R 3 is hydrogen or alkyl. (google.com.au)
- We investigated the effect of transforming growth factor beta 1 (TGF-β1) on equine hyaluronan synthase 2 (HAS2) gene expression and hyaluronan (HA) synthesis in culture models of articular chondrocytes. (bvsalud.org)
- M is a connecting group comprising a structure that does not interfere with the nucleic acid labeling compound's function. (google.com.au)
- Results:After 2 weeks of treatment in high-and low-dose osimertinib alone, tumor volume in the high-dose group was significantly less than in low-dose osimertinib-alone group (P0.05). (bvsalud.org)
- The bupropion hydrochloride sustained-release tablets was orally administered in the drug group for 7 weeks, 150 mg each time, once a day for the first 3 days, twice daily from day 4 to day 7, and once a day after day 8. (bvsalud.org)
- Activation of mast cells by bile acids. (naver.com)
- Exogenous nucleic acids induce an innate immune response in mammalian host cells through activation of the retinoic acid-inducible gene I (RIG-I). We evaluated RIG-I protein for RNA binding and ATPase stimulation with RNA ligands to investigate the correlation with the extent of immune response through RIG-I activation in cells. (bvsalud.org)
- However, hyperpolarization-activated ClC-2 channels expressed in heterologous expression systems bear a striking resemblance to the well-characterized hyperpolarization-activated cationic pacemaker current, I f (or I h ), found in many mammalian cardiac cell types 21 22 and are now known to belong to the HCN gene family. (ahajournals.org)
- One hundred twenty-eight male and female New Zealand white rabbits underwent intraperitoneal infection subsequent ileal anastomosis surgery were divided randomly into 4 groups, including 32 animals in each, applied with saline solution, FG, rhVEGF165 and a mixture of rhVEGF165 with FG (VEGF + FG) on the anastomoses, respectively. (bvsalud.org)
- We investigated in a physiological salt solution (PSS) containing HCO3- the intracellular pH (pHi) regulating mechanisms in smooth muscle cells cultured from human internal mammary arteries, using the pH-sensitive dye 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) and 22Na+ influx rates. (ahajournals.org)
- Finally, continuous exposure to 10% CO 2 in the presence of HCO 3 - produced intracellular acidification, and the rate of pH i recovery from intracellular acidosis was inhibited by Ba 2+ , which blocks pH i -induced changes in g K+ , and by 4-acetamido-4′-isothiocyanatostilbene-2,2′-disulfonic acid, which inhibits Na + /HCO 3 - cotransport. (elsevier.com)
- By contrast, in the presence of HCO 3 - , NH 4 Cl exposure and withdrawal produced a decrease in apparent buffering capacity and changes in membrane potential difference consistent with compensatory regulation of electrogenic Na + /HCO 3 - cotransport. (elsevier.com)