A non-penetrating amino reagent (commonly called SITS) which acts as an inhibitor of anion transport in erythrocytes and other cells.
An inhibitor of anion conductance including band 3-mediated anion transport.
A class of organic compounds that contains a naphthalene moiety linked to a sulfonic acid salt or ester.
A subclass of purinergic P2 receptors that signal by means of a ligand-gated ion channel. They are comprised of three P2X subunits which can be identical (homotrimeric form) or dissimilar (heterotrimeric form).
Compounds that bind to and block the stimulation of PURINERGIC P2 RECEPTORS.
This is the active form of VITAMIN B 6 serving as a coenzyme for synthesis of amino acids, neurotransmitters (serotonin, norepinephrine), sphingolipids, aminolevulinic acid. During transamination of amino acids, pyridoxal phosphate is transiently converted into pyridoxamine phosphate (PYRIDOXAMINE).
A class of cell surface receptors for PURINES that prefer ATP or ADP over ADENOSINE. P2 purinergic receptors are widespread in the periphery and in the central and peripheral nervous system.
A polyanionic compound with an unknown mechanism of action. It is used parenterally in the treatment of African trypanosomiasis and it has been used clinically with diethylcarbamazine to kill the adult Onchocerca. (From AMA Drug Evaluations Annual, 1992, p1643) It has also been shown to have potent antineoplastic properties.
A colorimetric reagent for iron, manganese, titanium, molybdenum, and complexes of zirconium. (From Merck Index, 11th ed)
Organic compounds that contain 1,2-diphenylethylene as a functional group.
Inorganic compounds derived from hydrochloric acid that contain the Cl- ion.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
Inorganic salts that contain the -HCO3 radical. They are an important factor in determining the pH of the blood and the concentration of bicarbonate ions is regulated by the kidney. Levels in the blood are an index of the alkali reserve or buffering capacity.
Membrane transporters that co-transport two or more dissimilar molecules in the opposite direction across a membrane. Usually the transport of one ion or molecule is against its electrochemical gradient and is "powered" by the movement of another ion or molecule with its electrochemical gradient.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Cell membrane glycoproteins that form channels to selectively pass chloride ions. Nonselective blockers include FENAMATES; ETHACRYNIC ACID; and TAMOXIFEN.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A disorder characterized by a reduction of oxygen in the blood combined with reduced blood flow (ISCHEMIA) to the brain from a localized obstruction of a cerebral artery or from systemic hypoperfusion. Prolonged hypoxia-ischemia is associated with ISCHEMIC ATTACK, TRANSIENT; BRAIN INFARCTION; BRAIN EDEMA; COMA; and other conditions.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
Inorganic compounds that contain bromine as an integral part of the molecule.
The semi-permeable outer structure of a red blood cell. It is known as a red cell 'ghost' after HEMOLYSIS.
Proteins encoded by the TAT GENES of the HUMAN IMMUNODEFICIENCY VIRUS.
Trans-acting transcription factors produced by retroviruses such as HIV. They are nuclear proteins whose expression is required for viral replication. The tat protein stimulates LONG TERMINAL REPEAT-driven RNA synthesis for both viral regulatory and viral structural proteins. tat stands for trans-activation of transcription.
The two lipoprotein layers in the MITOCHONDRION. The outer membrane encloses the entire mitochondrion and contains channels with TRANSPORT PROTEINS to move molecules and ions in and out of the organelle. The inner membrane folds into cristae and contains many ENZYMES important to cell METABOLISM and energy production (MITOCHONDRIAL ATP SYNTHASE).
The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte.
A multisubunit enzyme complex containing CYTOCHROME A GROUP; CYTOCHROME A3; two copper atoms; and 13 different protein subunits. It is the terminal oxidase complex of the RESPIRATORY CHAIN and collects electrons that are transferred from the reduced CYTOCHROME C GROUP and donates them to molecular OXYGEN, which is then reduced to water. The redox reaction is simultaneously coupled to the transport of PROTONS across the inner mitochondrial membrane.
Cytochromes of the c type that are found in eukaryotic MITOCHONDRIA. They serve as redox intermediates that accept electrons from MITOCHONDRIAL ELECTRON TRANSPORT COMPLEX III and transfer them to MITOCHONDRIAL ELECTRON TRANSPORT COMPLEX IV.
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)
A group of disorders caused by defective salt reabsorption in the ascending LOOP OF HENLE. It is characterized by severe salt-wasting, HYPOKALEMIA; HYPERCALCIURIA; metabolic ALKALOSIS, and hyper-reninemic HYPERALDOSTERONISM without HYPERTENSION. There are several subtypes including ones due to mutations in the renal specific SODIUM-POTASSIUM-CHLORIDE SYMPORTERS.
A condition caused by the overproduction of ALDOSTERONE. It is characterized by sodium retention and potassium excretion with resultant HYPERTENSION and HYPOKALEMIA.
Abnormally low potassium concentration in the blood. It may result from potassium loss by renal secretion or by the gastrointestinal route, as by vomiting or diarrhea. It may be manifested clinically by neuromuscular disorders ranging from weakness to paralysis, by electrocardiographic abnormalities (depression of the T wave and elevation of the U wave), by renal disease, and by gastrointestinal disorders. (Dorland, 27th ed)
A characteristic symptom complex.
A subclass of symporters that specifically transport SODIUM CHLORIDE and/or POTASSIUM CHLORIDE across cellular membranes in a tightly coupled process.
Genetic defects in the selective or non-selective transport functions of the KIDNEY TUBULES.
Potassium channels whose activation is dependent on intracellular calcium concentrations.
A disorder caused by hemizygous microdeletion of about 28 genes on chromosome 7q11.23, including the ELASTIN gene. Clinical manifestations include SUPRAVALVULAR AORTIC STENOSIS; MENTAL RETARDATION; elfin facies; impaired visuospatial constructive abilities; and transient HYPERCALCEMIA in infancy. The condition affects both sexes, with onset at birth or in early infancy.
A suspension of metallic gold particles.
A family of membrane-associated proteins responsible for the attachment of the cytoskeleton. Erythrocyte-related isoforms of ankyrin attach the SPECTRIN cytoskeleton to a transmembrane protein (ANION EXCHANGE PROTEIN 1, ERYTHROCYTE) in the erythrocyte plasma membrane. Brain-related isoforms of ankyrin also exist.
A heterogeneous group of disorders characterized by renal electrolyte transport dysfunctions. Congenital forms are rare autosomal disorders characterized by neonatal hypertension, HYPERKALEMIA, increased RENIN activity and ALDOSTERONE concentration. The Type I features HYPERKALEMIA with sodium wasting; Type II, HYPERKALEMIA without sodium wasting. Pseudohypoaldosteronism can be the result of a defective renal electrolyte transport protein or acquired after KIDNEY TRANSPLANTATION.
A variant of the GUILLAIN-BARRE SYNDROME characterized by the acute onset of oculomotor dysfunction, ataxia, and loss of deep tendon reflexes with relative sparing of strength in the extremities and trunk. The ataxia is produced by peripheral sensory nerve dysfunction and not by cerebellar injury. Facial weakness and sensory loss may also occur. The process is mediated by autoantibodies directed against a component of myelin found in peripheral nerves. (Adams et al., Principles of Neurology, 6th ed, p1313; Neurology 1987 Sep;37(9):1493-8)
Electroneutral chloride bicarbonate exchangers that allow the exchange of BICARBONATE IONS exchange for CHLORIDE IONS across the cellular membrane. The action of specific antiporters in this class serve important functions such as allowing the efficient exchange of bicarbonate across red blood cell membranes as they passage through capillaries and the reabsorption of bicarbonate ions by the kidney.
Glands of external secretion that release its secretions to the body's cavities, organs, or surface, through a duct.
Substances that dissociate into two or more ions, to some extent, in water. Solutions of electrolytes thus conduct an electric current and can be decomposed by it (ELECTROLYSIS). (Grant & Hackh's Chemical Dictionary, 5th ed)
Ability of ERYTHROCYTES to change shape as they pass through narrow spaces, such as the microvasculature.
Oxygen-carrying RED BLOOD CELLS in mammalian blood that are abnormal in structure or function.
The solid substance formed by the FREEZING of water.
Anemia characterized by larger than normal erythrocytes, increased mean corpuscular volume (MCV) and increased mean corpuscular hemoglobin (MCH).
Positively charged atoms, radicals or groups of atoms which travel to the cathode or negative pole during electrolysis.
An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.
... disulfonic acid (DIDS), a chloride (Cl−) channel blocker, was injected into chronic cerebral ischemia-hypoxia rat models at ... Furthermore, the elevated number of caspase-3 and neural/glial antigen 2 (NG-2) double-labeled positive cells was attenuated by ... Our results showed that DIDS significantly reduced the elevated mRNA levels and protein expression of chloride channel 2 (ClC-2 ... disulfonic acid (DIDS), a chloride (Cl−) channel blocker, was injected into chronic cerebral ischemia-hypoxia rat models at ...
... disulfonic acid (DIDS). The kinetics of the residual chloride transport (0.2%, 106 pmol.cm-2 x s-1) at 38 degrees C, pH 7.2) ... diisothiocyanostilbene-2,2-disulfonic acid binding.. P K Gasbjerg, J Funder, J Brahm ... The outside apparent affinity, expressed by Ko1/2,c, was 34 mM, as determined by substituting external KCl by sucrose. The ... Kinetics of residual chloride transport in human red blood cells after maximum covalent 4,4-diisothiocyanostilbene-2,2- ...
Disulfonic Acid" was a major or minor topic of these publications. To see the data from this visualization as text, click here. ... Disulfonic Acid" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... Disulfonic Acid" by people in this website by year, and whether "4,4-Diisothiocyanostilbene-2,2- ... Below are the most recent publications written about "4,4-Diisothiocyanostilbene-2,2-Disulfonic Acid" by people in Profiles. ...
... disulfonic acid (DIDS) protects isolated mitochondria against Tat-induced mitochondrial membrane permeabilization (MMP), ... diisothiocyanostilbene-2,2- ... Proto-Oncogene Proteins c-bcl-2 * tat Gene Products, Human ... Pharmacologic inhibitors of the permeability transition pore, Bax/Bak inhibitors, and recombinant Bcl-2 and Bcl-XL proteins do ...
... disulfonic Acid / analogs & derivatives * 4-Acetamido-4-isothiocyanatostilbene-2,2-disulfonic Acid / pharmacology ... Diisothiocyanostilbene-2,2-Disulfonic Acid * 4-Acetamido-4-isothiocyanatostilbene-2,2- ... Rather, its preferential site of action and that of iodoacetic acid were identified by radiolabeling studies and microsequence ... acetate exhibits a much lower capacity for cell entry and that the structural analog alpha-cyano-3-hydroxycinnamic acid ...
... disulfonic acid. NHE. =. sodium-proton exchange. PKA. =. protein kinase A. PKC. =. protein kinase C. ... diisothiocyano-stilbene-2,2′- ... Novelty and Significance: 1) What Is New, 2) What Is Relevant? ... Na+/H+ and Cl−/HCO3− exchanges were enhanced in erythrocytes of all patients with Bartter-like syndrome (Table 2⇓) partly due ... Two hundred micromoles per liter of the red blood cell suspension was treated with 4 mCi/L 86Rb as a radioactive analogue of ...
... disulfonic acid. BSP. sulfobromophthalein. CDNB. 1-choloro-2,4-dinitrobenzene. 2-DG. 2-deoxyglucose. A. apical. S. serosal. M. ... diisothiocyanostilbene-2,2′- ... 2,4-dinitrophenyl-S-glutathione. SD rat. Sprague-Dawley rat. ... Permeability of ME3277 across monolayer of Caco-2 cells with P-glycoprotein (P-gp) and indomethacin-sensitive efflux pump ... The efflux transport of ME3277 was inhibited by 1-naphthol, 1-choloro-2,4-dinitrobenzene, and sulfobromophthalein, and efflux ...
... disulfonic acid, but not by small anionic compounds, such as lactic acid and acetic acid. The inhibitory effect of pravastatin ... diisothiocyanostilbene-2,2′- ...
... disulfonic acid (2) * adult (2) * bodily secretions (2) Filters. * Has Multimedia (1) ... diisothiocyanostilbene-2,2- ... Tissue-specific expression of alpha-2-macroglobulin by choroid ...
... disulfonic acid), ouabain, rotenone, BSA, and alanine-proline were purchased from Sigma Chemical Co. (St. Louis, MO). KCN was ... diisothiocyanostilbene-2, 2′- ... the monocarboxylic acid benzoic acid (100 μM), the nonspecific ... As both enalaprilat and temocaprilat are dicarboxylic acids and enalapril is a monocarboxylic acid, it is plausible that ... Benzoic acid, a putative substrate of the monocarboxylate transporter MCT2 (Garcia et al., 1995) and not of Oatp1 (Pang et al ...
... disulfonic acid binding. J Gen Physiol. 1993 May101(5):715-32.PubMed ID8393066 ... diisothiocyanostilbene-2,2- ... Fig.2), the apparent permeability was reduced to 1.2×10^-6cm/ ... Gasbjerg PK, Funder J, Brahm J. Kinetics of residual chloride transport in human red blood cells after maximum covalent 4,4- ... The permeability calculated from the irreversible-DIDS-inhibited bicarbonate efflux (Fig.2) is 1.7×10^-6 cm/sec at pH 7.8, ...
... disulfonic acid. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009]. Expression. ... diisothiocyanostilbene-2,2- ... Transcript Variant: This variant (4) differs in the 5 UTR ... Variants 1, 2, 3 and 4 encode the same protein.. Source sequence(s). AI963799, AK075248, AK315132, BC035900, DA290933 Consensus ... Transcript Variant: This variant (3) differs in the 5 UTR compared to variant 1. Variants 1, 2, 3 and 4 encode the same ...
... disulfonic acid (DIDS) was obtained from Invitrogen. The bath solution for HEK293 cells (solution A) contained 145 mM NaCl, 1 ... diisothiocyanostilbene-2,2′-disulfonic acid; GFP, green fluorescent protein; HEK, human embryonic kidney. ... 2 (C and D), we measured the Clo− dependency of HCO3− and Cl− transport. To measure the transport at close to the resting pHi ... 2 D. It is clear that the slc26a3-mediated Cl− and HCO3− fluxes have the same dependency on Clo−. Interestingly, the Clo− ...
... disulfonic acid (DIDS), and anthracene-9-carboxylic acid (A-9-C), on Ca2+ signalling in rat pulmonary artery smooth muscle ... Diisothiocyanostilbene-2,2-disulfonic acid. *Furthermore, Trk1p-mediated chloride conductance was blocked by 4,4- ... disulfonic acid inhibits CD3-T cell antigen receptor-stimulated Ca2+ influx in human T lymphocytes. Rosoff, P.M., Hall, C., ... disulfonic acid of the plasma membrane (Ca(2+)+Mg2+)ATPase from kidney proximal tubules. Guilherme, A., Meyer-Fernandes, J.R., ...
... disulfonic acid (DIDS) and its dihydro derivative (H2DIDS) J Membr Biol 29(1-2):147-77. ... diisothiocyanostilbene-2,2′-disulfonic acid, disodium salt (DIDS). Aliquots were taken for ektacytometry or gradient ... stilbene disulfonic acid derivatives), a chloride channel inhibitor NS1652, and the inhibitor of the KNHE, HOE-642. Our data ... of band 3 have been found to be less efficient in transporting anions and have a decreased affinity to stilbene disulfonic acid ...
... disulfonic acid on apical efflux and cellular retention. Ulrika Lindencrona, Eva Forssell-Aronsson, Mikael Nilsson Nuclear ... diisothiocyanostilbene-2,2- ... 5th Swedish Cancer Research Meeting, Umeå, 2019, 3-4 October - ... Radiation Induces Up-Regulation of Somatostatin Receptors 1, 2, and 5 in Small Cell Lung Cancer In Vitro Also at Low Absorbed ... Differential expression of vesicular monoamine transporter (VMAT) 1 and 2 in gastrointestinal endocrine tumours. ...
... disulfonic acid and protonophore carbonylcyanide p-trifluoromethoxyphenylhydrazone, respectively. These observations ... diisothiocyanostilbene-2,2- ... The initial uptakes of L-[(14)C]lactic acid which are driven by ... Anion antiport mechanism is involved in transport of lactic acid across intestinal epithelial brush-border membrane.. Tamai I1 ... Kinetic analysis of L-[(14)C]lactic acid uptake revealed the involvement of two saturable processes in the presence of both ...
... disulfonic acid (DIDS) and an antibody against the ClC-3 channels, but accelerated by increases in the intracellular ATP:ADP ... diisothiocyanostilbene-2,2- ... The cyanobacterial amino acid β-N-methylamino-L-alanine (BMAA) ... The amino acid sequence of eEF2 is highly conserved in different organisms. eEF2 from S. cerevisiae contains 842 amino acids. ... The amino acid sequence of eEF2 is highly conserved in different organisms. eEF2 from S. cerevisiae contains 842 amino acids. ...
... disulfonic acid (DIDS) stimulates K+ efflux into the portal space in proportion to choleresis (13), suggesting a functional ... diisothiocyanostilbene-2,2′- ... The overall sequence identity at the amino acid level is 90%. ... The longer deduced isoform (4.2a) has 30 additional NH3-terminal amino acids, which identifies this as a new isoform. The short ... A maltose-binding protein/Kir4.2 fusion protein containing the final, COOH-terminal 170 amino acid residues of the channel was ...
Thus, fugu-NBCe1 represents the first ortholog-based tool to study amino acid substitutions in NBCe1 and how those change ion ... Thus, fugu-NBCe1 represents the first ortholog-based tool to study amino acid substitutions in NBCe1 and how those change ion ... Thus, fugu-NBCe1 represents the first ortholog-based tool to study amino acid substitutions in NBCe1 and how those change ion ... Thus, fugu-NBCe1 represents the first ortholog-based tool to study amino acid substitutions in NBCe1 and how those change ion ...
Unlabeled biotin and the structural analogs with free carboxyl groups (thioctic acid, desthiobiotin) but not those with blocked ... Unlabeled biotin and the structural analogs with free carboxyl groups (thioctic acid, desthiobiotin) but not those with blocked ... Unlabeled biotin and the structural analogs with free carboxyl groups (thioctic acid, desthiobiotin) but not those with blocked ... Unlabeled biotin and the structural analogs with free carboxyl groups (thioctic acid, desthiobiotin) but not those with blocked ...
2#-disulfonic acid, 100 mm Tricine-KOH, pH 8.0). Control experiments with membranes from yeast cells harboring the empty vector ... 4#-diisothiocyanostilbene-2,2#-disulfonic acid also effectively inhibited GspPT-mediated import of [32P]phosphate (data not ... 2A), AMP, UMP, gluconate-6-P, ribulose-1,5-bisP, ADP-Glc, UDP-Glc, UDP-Gal, α-ketoglutaric acid, malic acid, pyruvic acid, or ... fatty acids, several amino acids, nucleic acids, and the reductive assimilation of inorganic ions like nitrate and sulfate ( ...
... disulfonic acid (DIDS) and bromosulphalein (BSP). We tested the efficiency of these drugs on HSP70 secretion and confirmed the ... diisothiocyanostilbene-2,2- ... Serum adipocyte fatty acid binding protein as a new biomarker ... Adipocyte fatty acid-binding protein is released from adipocytes by a non-conventional mechanism. Int. J. Obes. 38:1251-1254. ... Identification of fatty acid binding protein 4 as an adipokine that regulates insulin secretion during obesity. Mol. Metab. 3: ...
2#-disulfonic acid, 100 mm Tricine-KOH, pH 8.0). Control experiments with membranes from yeast cells harboring the empty vector ... 4#-diisothiocyanostilbene-2,2#-disulfonic acid also effectively inhibited GspPT-mediated import of [32P]phosphate (data not ... 2A), AMP, UMP, gluconate-6-P, ribulose-1,5-bisP, ADP-Glc, UDP-Glc, UDP-Gal, α-ketoglutaric acid, malic acid, pyruvic acid, or ... fatty acids, several amino acids, nucleic acids, and the reductive assimilation of inorganic ions like nitrate and sulfate ( ...
... disulfonic acid (DIDS) to block non-CFTR Cl− channels, followed by forskolin and IBMX to increase cellular concentrations of ... diisothiocyanostilbene-2,2′- ... E) to (H) are H&E stain and (I) to (M) are periodic acid-Schiff ... Protein was measured with the bicinchoninic acid (BCA) assay (Thermo Fisher). Protein (10 to 25 mg) was used per lane as ... CFTR+/+ (n = 5) combined with CFTR+/ΔF508 (n = 4); CFTRΔF508/ΔF508 (n = 11). Compared to an earlier study that showed ...
The researchers found that, in macrophages from Alzheimers patients, 1,25D3 potentiated the 4,4-diisothiocyanostilbene-2,2- ... disulfonic acid-sensitive chloride channel (CIC-3) currents in type I and II cells. In addition, 1,25D3 upregulated CIC-3 mRNA ...
... isothiocyanostilbene disulfonic acid pHi intracellular pH pHe extracellular pH PAGE 12. 12 Abstract of Dissertation presented ... diisothiocyanostilbene 2,2 disulfonic acid (DIDS) was purchased from Sigma Aldrich; 4 acetamido 4 isothiocyanostilbene 2,2 ... The coincidence of high lactic acid out put and low pHe has led to a popular belief that lactic acid is the source of the acid ... The pHi was measured using the distribution of the weak acid [7 14 C] benzoic acid as describ ed in in Chapter 2. The pHi of ...
The quinolines 2-(4-methylphenyl)-6-methyl quinoline (THQ-4S) and 2-(4-aminophenyl)-6-methylquinoline (THQ-55) inhibited …in ... Genomic and Nongenomic Signaling Induced by 1α,25(OH) 2-Vitamin D 3 Promotes the Recovery of Amyloid-β Phagocytosis by ... 2 -vitamin D3 (1,25D3) and blocked by the nuclear vitamin D receptor (VDR) antagonist (23S)-25-dehydro-1α(OH)-vitamin D3 -26,23 ... 2-disulfonic acid-sensitive chloride channel (i.e., ClC-3) currents in both Type I and II AD macrophages, but curcumin only ...
... disulfonic acid (H2-DIDS), but not by a specific CFTR inhibitor, CFTR(inh)-172. Our data show that SeV vector is a potential ... The cAMP stimulated Cl-/HCO3- exchange was inhibited by dihydro-4,4-diisothiocyanostilbene-2,2- ... diisothiocyanostilbene-2,2-disulfonic acid (H2-DIDS), but not by a specific CFTR inhibitor, CFTR(inh)-172. Our data show that ... The cAMP stimulated Cl-/HCO3- exchange was inhibited by dihydro-4,4- ...
  • In this study, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), a chloride (Cl − ) channel blocker, was injected into chronic cerebral ischemia-hypoxia rat models at different time points. (mdpi.com)
  • Our results showed that DIDS significantly reduced the elevated mRNA levels and protein expression of chloride channel 2 (ClC-2) in neonatal rats induced by ischemia-hypoxia. (mdpi.com)
  • Furthermore, the elevated number of caspase-3 and neural/glial antigen 2 (NG-2) double-labeled positive cells was attenuated by DIDS after ischemia anoxic injury. (mdpi.com)
  • Irreversible inhibition, 99.8% of control values for chloride transport in human red blood cells, was obtained by well-established methods of maximum covalent binding of 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS). (rupress.org)
  • The anion-channel inhibitor 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) protects isolated mitochondria against Tat-induced mitochondrial membrane permeabilization (MMP), whereas ruthenium red, a ryanodine receptor blocker, does not. (nih.gov)
  • The permeability calculated from the irreversible-DIDS-inhibited bicarbonate efflux (Fig.2) is 1.7×10^-6 cm/sec at pH 7.8, almost twice as high as the permeability for irreversible DIDS-inhibited chloride efflux from intact red blood cells (P=1.0×10^-6 cm/sec, primary source). (harvard.edu)
  • dids 5-isothiocyanato-2-[(E)-2- (4. (wikigenes.org)
  • Furthermore, the divalent organic anion 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid (DIDS) stimulates K + efflux into the portal space in proportion to choleresis ( 13 ), suggesting a functional coupling between sinusoidal K + and canalicular anion efflux. (physiology.org)
  • Accordingly, granule acidification and priming are inhibited by agents that prevent transgranular Cl(-) fluxes, such as 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) and an antibody against the ClC-3 channels, but accelerated by increases in the intracellular ATP:ADP ratio or addition of hypoglycemic sulfonylureas. (diva-portal.org)
  • The cAMP stimulated Cl-/HCO3- exchange was inhibited by dihydro-4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (H2-DIDS), but not by a specific CFTR inhibitor, CFTR(inh)-172. (cfgenetherapy.org.uk)
  • MTT assay showed that SIN-1 could decrease the cell viability of rat hippocampal neurons, which could be restored by the chloride channel inhibitor, DIDS (4, 4'-diisothiocyanostilbene-2, 2'- disulfonic acid). (alliedacademies.org)
  • Moreover, VDAC inhibitor 4, 4'-diisothiocyano stilbene-2, 2'-disulfonic acid (DIDS) alleviated QCT-induced cell death and VDAC2 oligomerization. (readbyqxmd.com)
  • 4) Intracellular alkalinization led to a depolarization (delta V = 24.7 +/- 2.3 mV), and intracellular acidification resulted in a hyperpolarization (delta V = 9.4 +/- 1.1 mV) that was inhibited by DIDS and dependent on extracellular HCO3(-)-CO2 and sodium. (uni-regensburg.de)
  • Activated by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) in a sustained intracellular acidification-dependence manner. (genecards.org)
  • Elevating external Ca 2+ (0.2 to 2 mM) neutralized the inhibition of DIDS. (ntnu.edu.tw)
  • Both the currents and pH(i) recovery were blocked by 200 microM 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS). (ox.ac.uk)
  • pHi recovery from an acute acid load was dependent on external Na+ and partially inhibited by the absence of HCO3(-) [N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES)-buffered solution] or by the anion transport inhibitor 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS). (mysciencework.com)
  • Kísérleteink célja az volt, hogy összehasonlítsuk két ismert gátlószer, a 9-antracén karboxilsav (9 AC) és a 4,4'-diisotiocianostilbén-2,2'-diszulfonsav (DIDS) hatásait, és eldöntsük, hogy melyik alkalmasabb a ICl(Ca) detektálására. (unideb.hu)
  • Megállapítottuk, hogy a 9-AC (0,5 mmol/l) és a DIDS (0,2 mmol/l) is növelte az AP időtartamot. (unideb.hu)
  • In this study we aimed to explore which blocker, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) or 9-anthracene carboxylic acid (9 AC) is preferable to visualize the profile of ICl(Ca). We aimed to study ICl(Ca) at normal Ca2+ cycling and by AP voltage-clamp as well as in conditions designed to change [Ca2+]i in canine ventricular myocytes. (unideb.hu)
  • The effect of Cl(-) channel (10 µmol/L niflumic acid and 1 mmol/L 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS)), K(+) channel (10 mmol/L tetraethylammonium chloride (TEA)), or Na(+) channel blockers (1 mmol/L amiloride) on 8-bromo-cAMP-induced Isc change was also studied. (bvsalud.org)
  • Finally, niflumic acid and DIDS, but not TEA or amiloride, significantly prevented the Isc increase induced by 8-bromo-cAMP. (bvsalud.org)
  • Using a combination of human B lymphoid leukemia cells and mouse models, we now show that AID expression can be harnessed for antileukemic effect, after inhibition of the RAD51 homologous recombination (HR) factor with 4,4'-diisothiocyanatostilbene-2-2'-disulfonic acid (DIDS). (jax.org)
  • In this work, we report that transient or stable expression of human NBC3 (hNBC3) in HEK cells resulted in a Na + -dependent, DIDS (4,4′-diisothiocyanostilbene-2,2′-disulfonic acid)- and 5-ethylisopropylamiloride-insensitive HCO 3 - transport. (elsevier.com)
  • Blockade of KCa3.1, low Cl - in the medium, and low dose of 4,4′-diisothiocyano-2,2′-stilbenedisulfonic acid (DIDS) impaired CCL19/CCL21-induced Ca 2+ influx, cell volume change, and DC migration. (elsevier.com)
  • route blockers, including diisothiocyanostilbene-2,20-disulfonic acidity (DIDS), 5-nitro-2C3-phenylpropylamino benzoic acidity (NPPB), and R(+)-indyanyloxyacetic acidity 94 (RIAA) (3) will be expected to impact MCPyV ST-induced cell motility. (cp-724714.com)
  • 3 H]Estrone-3-sulfate transport was decreased by pravastatin, aromatic anion compounds, and the anion exchange inhibitor 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid, but not by small anionic compounds, such as lactic acid and acetic acid. (aspetjournals.org)
  • Anion antiport mechanism is involved in transport of lactic acid across intestinal epithelial brush-border membrane. (nih.gov)
  • Intestinal epithelial membrane transport of L-lactic acid was characterized using rabbit jejunal brush-border membrane vesicles (BBMVs). (nih.gov)
  • The uptake of L-[(14)C]lactic acid by BBMVs showed an overshoot phenomenon in the presence of outward-directed bicarbonate and/or inward-directed proton gradients. (nih.gov)
  • Kinetic analysis of L-[(14)C]lactic acid uptake revealed the involvement of two saturable processes in the presence of both proton and bicarbonate gradients. (nih.gov)
  • An arginyl residue-modifying agent, phenylglyoxal, inhibited L-[(14)C]lactic acid transport by the proton cotransporter, but not by the anion antiporter. (nih.gov)
  • The initial uptakes of L-[(14)C]lactic acid which are driven by bicarbonate ion and proton gradients were inhibited commonly by monocarboxylic acids and selectively by anion exchange inhibitor 4, 4'-diisothiocyanostilbene-2,2'-disulfonic acid and protonophore carbonylcyanide p-trifluoromethoxyphenylhydrazone, respectively. (nih.gov)
  • These observations demonstrate that L-lactic acid is transported across the intestinal brush-border membrane by multiple mechanisms, including an anion antiporter and a previously known proton cotransporter. (nih.gov)
  • We provided evidence that CAIX contributes to metabolic dysfunction through studies on pH, lactic acid production, glucose uptake, and CAIX inhibition. (ufl.edu)
  • The researchers found that, in macrophages from Alzheimer's patients, 1,25D3 potentiated the 4,4-diisothiocyanostilbene-2,2-disulfonic acid-sensitive chloride channel (CIC-3) currents in type I and II cells. (empr.com)
  • Among its related pathways are Salivary secretion and Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds . (genecards.org)
  • It was found that MDR1 activity and its inhibition by cyclosporine A or flufenamic acid were unaffected by hypotonicity alone or in combination with Cl - channel blockers. (elsevier.com)
  • 5-Nitro-2-(3-phenyl-propylamino)benzoic acid (NPPB), furosemide, and niflumate blocked the malaria-induced transport of monovalent cations, neutral amino acids, sugars, nucleosides, and monovalent anions. (ox.ac.uk)
  • The resulting proteoliposomes transport CMP-N-acetylneuraminic acid (CMP-AcNeu) and adenosine 3'-phosphate 5'-phosphosulfate with very similar affinity and inhibition characteristics as intact Golgi vesicles. (umassmed.edu)
  • 4,4'-Diisothiocyanatostilbene-2,2'-disulphonic acid (100 microM) produced a rapid and reversible voltage-dependent block (60 +/- 5% and 10 +/- 7% inhibition of current, measured at +/- 60 mV, respectively). (ox.ac.uk)
  • In the present study, fatty acid (FA) transport is characterized with regard to its specificity and susceptibility to inhibition by protein modifiers. (wustl.edu)
  • The outside apparent affinity, expressed by Ko1/2,c, was 34 mM, as determined by substituting external KCl by sucrose. (rupress.org)
  • 1 Mutated forms of band 3 have been found to be less efficient in transporting anions and have a decreased affinity to stilbene disulfonic acid derivatives. (haematologica.org)
  • Thus, fugu-NBCe1 represents the first ortholog-based tool to study amino acid substitutions in NBCe1 and how those change ion and voltage dependence. (elsevier.com)
  • Multiple alignment of AkMLC2f amino acid sequences with MLC2 from other species. (bioz.com)
  • Nucleotide and deduced amino acid sequences of Akmlc2f cDNA. (bioz.com)
  • 5) pHi backregulation after an acid load occurred in both the presence and absence of extracellular bicarbonate but not in the absence of extracellular sodium. (uni-regensburg.de)
  • We have functionally characterized Na+-driven bicarbonate transporter (NBC)4, originally cloned from human heart by Pushkin et al. (ox.ac.uk)
  • The anion exchanger (AE) plays critical roles in physiological processes including CO 2 transport and volume regulation in erythrocytes and acid-base regulation in renal tubules. (ntnu.edu.tw)
  • Citrate excretion was sensitive to anion channel blockers, such as niflumic acid and anthracene-9-carboxylic acid. (oup.com)
  • Mechanism of short-chain fatty acid uptake by apical membrane vesicles of rat distal colon. (naver.com)
  • 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (wakehealth.edu)
  • Kinetics of residual chloride transport in human red blood cells after maximum covalent 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid binding. (rupress.org)
  • The kinetics of the residual chloride transport (0.2%, 106 pmol.cm-2 x s-1) at 38 degrees C, pH 7.2) was studied by means of 36Cl- efflux. (rupress.org)
  • The mutant protein is retained in the endoplasmic reticulum (ER) and rapidly degraded ( 2 - 4 ), and as a result, CFTR-ΔF508 fails to reach the apical membrane. (sciencemag.org)
  • Fingerprint Dive into the research topics of 'Permeation of long-chain fatty acid into adipocytes. (wustl.edu)
  • An appreciation of the functional properties of the cytoplasmic fatty acid binding protein 4 (FABP4) has advanced with the recent demonstration that an extracellular form secreted by adipocytes regulates a wide range of physiological functions. (rupress.org)
  • Permeation of long-chain fatty acid into adipocytes. (wustl.edu)
  • Pronase but not trypsin treatment of intact cells reduces fatty acid influx. (wustl.edu)
  • 3. The transgenic pig of claim 2, wherein said intestinal cell-specific promoter is an intestinal fatty acid binding protein (iFABP) promoter. (patentsencyclopedia.com)
  • Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. (genecards.org)
  • Pharmacologic inhibitors of the permeability transition pore, Bax/Bak inhibitors, and recombinant Bcl-2 and Bcl-XL proteins do not reduce Tat-induced MMP. (nih.gov)
  • Polyacrylamide gel electrophoresis of plasma membrane proteins from cells treated with [ 3 H] 4,4'-diisothiocyanostilbene-2,2'-disulfonate shows a peak of radioactivity at about M(r) = 85,000. (wustl.edu)
  • MCPyV ST features as an inhibitor of NF-BCmediated transcription (11) and network marketing leads towards the Salvianolic acid D manufacture hyperphosphorylation from the translation regulatory proteins, 4E-BP1, leading to dysregulation of cap-dependent translation (10). (cp-724714.com)
  • Sialic acid and sodium sulfate, which are transported only very slowly into the lumen of Golgi vesicles, are transported at low rates by the reconstituted proteoliposomes. (umassmed.edu)
  • The encoded protein is a sodium independent sulfate transporter that is sensitive to the anion exchanger inhibitor 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid. (nih.gov)
  • Permeability of ME3277 across monolayer of Caco-2 cells with P-glycoprotein (P-gp) and indomethacin-sensitive efflux pump expression did not show any directionality and verapamil, an inhibitor of P-gp, and indomethacin did not affect the permeability of ME3277 across rat intestinal tissue. (aspetjournals.org)
  • The efflux transport of ME3277 was inhibited by 1-naphthol, 1-choloro-2,4-dinitrobenzene, and sulfobromophthalein, and efflux of ME3277 and monoacid metabolites from intestinal tissue preloaded with ME3229 fell in the presence of 1-naphthol and sulfobromophthalein. (aspetjournals.org)
  • 2. The transgenic pig of claim 1, wherein the promoter is intestinal cell-specific. (patentsencyclopedia.com)
  • It is strongly and irreversibly blocked by pretreatment of the cells with the stilbene compounds, 4,4'-diisothiocyanostilbene-2,2'-disulfonate and 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid, but only slightly inhibited by dipyridamole. (wustl.edu)
  • A biologic function for an "orphan" messenger: D-myo-inositol 3,4,5,6-tetrakisphosphate selectively blocks epithelial calcium-activated chloride channels. (ac.be)
  • We reported that amyloid beta peptide (Abeta42) activated neutral sphingomyelinase 2 (nSMase2) thereby increasing the concentration of the sphingolipid ceramide in astrocytes. (readbyqxmd.com)
  • Intrathecal injection of a VDAC1 inhibitor significantly reversed the pain hypersensitivity and reduced the over-expression of Toll-like receptor 4 (TLR4). (readbyqxmd.com)
  • Concanamycin A, an inhibitor of V-type ATPase, had no effect on the rates of acid extrusion. (edu.au)
  • These acid-loading and acid-extruding transport mechanisms together with the Na(+), K(+), ATPase may be sufficient to account not only for pH(i) regulation in brain endothelial cells but also for the net secretion of HCO(-)(3) across the blood-brain barrier. (edu.au)
  • Requires for normal levels of gastric acid secretion, secretory membrane development, parietal cell maturation and/or differentiation and at least secondarily for chief cell differentiation (By similarity). (genecards.org)
  • The kidney biopsies of patients with Bartter's syndrome showed juxtaglomerular hyperplasia, and elevated prostaglandin levels were found in their blood 2 and urine. (ahajournals.org)
  • Following relatively small acid loads with pH(i) approximately 6.5, HCO(-)(3) influx accounted for most of the acid extrusion from the cell with both Cl(-)-independent and Cl(-)-dependent, Na(+)-dependent transporters involved. (edu.au)
  • The longer deduced isoform (4.2a) has 30 additional NH 3 -terminal amino acids, which identifies this as a new isoform. (physiology.org)
  • The findings that [14C] acetate exhibits a much lower capacity for cell entry and that the structural analog alpha-cyano-3-hydroxycinnamic acid inhibits pyruvate release provide additional support for the presence of a pyruvate transporter. (nih.gov)
  • and was later shown to function as a SO 4 = transporter. (rupress.org)
  • The Cl(-)-independent component has the same properties as the NBC-like transporter previously shown to account for most of the acid extrusion near the resting pH(i). (edu.au)
  • This exchange had the same properties as the AE-like transporter previously identified as a major acid loader near resting pH(i). (edu.au)
  • Transport activities involved in intracellular pH (pH(i)) recovery after acid or alkali challenge were investigated in cultured rat brain microvascular endothelial cells by monitoring pH(i) using a pH-sensitive dye. (edu.au)
  • Intracellular Cl- depletion of arteries did not affect the rate of pHi recovery in PSS from an acid load. (mysciencework.com)
  • These data suggest that human small arteries maintain pHi by Na+/H+ exchange and Na(+)-dependent HCO3(-) exchange in response to an acid load, and Na(+)-independent Cl-/HCO3(-) exchange to counteract intracellular alkalosis. (mysciencework.com)
  • After correcting for a 4.4-fold lower apparent Stern-Volmer constant for intravesicular lucigenin, a maximum rate of Cl(-) transport of 31 nmol m(-2) sec(-1) was calculated, in good agreement with values obtained for the plant vacuolar membrane using other techniques. (ox.ac.uk)
  • Although voltage dependent anion channel 1 (VDAC1) involved in regulating QCT-induced apoptotic cell death has been established, the role of voltage dependent anion channel 2 (VDAC2) in QCT-induced toxicity remains unclear. (readbyqxmd.com)
  • The rate of citrate release from IPG cells was not affected by the concentration of aluminum (0 and 50 µM), soluble P i (0 or 2 mM) and the pH (4.5-5.6) of the medium, suggesting that anion channels would not be regulated by such external conditions. (oup.com)
  • The cell viability was assessed by the MTT (3-(4, 5-Dimethythiazol-2 yl)- diphenytetra zolium bromide) assay. (alliedacademies.org)
  • Fig.2), the apparent permeability was reduced to 1.2×10^-6cm/sec. (harvard.edu)
  • Sensory neurons signal for an increase in rat gastric mucosal blood flow in the face of pending acid injury. (naver.com)
  • At 24 h after transfection, the cells were fixed with 4% paraformaldehyde in PBS (pH 7.4), mounted using VECTASHIELD ® with DAPI, and viewed using a confocal laser scanning microscope. (bioz.com)
  • In this study, we found that the expression of VDAC1 was upregulated in the L2-5 segments of the spinal dorsal horn at 2 and 3 weeks after injection of tumor cells into the tibial cavity. (readbyqxmd.com)
  • Activation of mast cells by bile acids. (naver.com)
  • Ki67 immunoreactivity, 5-ethynyl-2'-deoxyuridine incorporation, and cell-cycle analysis of cells grown in low-Cl(-) media showed fewer proliferating cells than in cultures grown in regular medium. (digestivediseasescenters.org)
  • Salvianolic acid D manufacture That is also backed by recent research displaying that engraftment of MCC cell lines into SCID mice leads to circulating tumor cells and metastasis development (16). (cp-724714.com)