Transformation mediated by RhoA requires activity of ROCK kinases. (1/10733)
BACKGROUND: The Ras-related GTPase RhoA controls signalling processes required for cytoskeletal reorganisation, transcriptional regulation, and transformation. The ability of RhoA mutants to transform cells correlates not with transcription but with their ability to bind ROCK-I, an effector kinase involved in cytoskeletal reorganisation. We used a recently developed specific ROCK inhibitor, Y-27632, and ROCK truncation mutants to investigate the role of ROCK kinases in transcriptional activation and transformation. RESULTS: In NIH3T3 cells, Y-27632 did not prevent the activation of serum response factor, transcription of c-fos or cell cycle re-entry following serum stimulation. Repeated treatment of NIH3T3 cells with Y-27632, however, substantially disrupted their actin fibre network but did not affect their growth rate. Y-27632 blocked focus formation by RhoA and its guanine-nucleotide exchange factors Dbl and mNET1. It did not affect the growth rate of cells transformed by Dbl and mNET1, but restored normal growth control at confluence and prevented their growth in soft agar. Y-27632 also significantly inhibited focus formation by Ras, but had no effect on the establishment or maintenance of transformation by Src. Furthermore, it significantly inhibited anchorage-independent growth of two out of four colorectal tumour cell lines. Consistent with these data, a truncated ROCK derivative exhibited weak ability to cooperate with activated Raf in focus formation assays. CONCLUSIONS: ROCK signalling is required for both the establishment and maintenance of transformation by constitutive activation of RhoA, and contributes to the Ras-transformed phenotype. These observations provide a potential explanation for the requirement for Rho in Ras-mediated transformation. Moreover, the inhibition of ROCK kinases may be of therapeutic use. (+info)Polarized distribution of Bcr-Abl in migrating myeloid cells and co-localization of Bcr-Abl and its target proteins. (2/10733)
Bcr-Abl plays a critical role in the pathogenesis of Philadelphia chromosome-positive leukemia. Although a large number of substrates and interacting proteins of Bcr-Abl have been identified, it remains unclear whether Bcr-Abl assembles multi-protein complexes and if it does where these complexes are within cells. We have investigated the localization of Bcr-Abl in 32D myeloid cells attached to the extracellular matrix. We have found that Bcr-Abl displays a polarized distribution, colocalizing with a subset of filamentous actin at trailing portions of migrating 32D cells, and localizes on the cortical F-actin and on vesicle-like structures in resting 32D cells. Deletion of the actin binding domain of Bcr-Abl (Bcr-AbI-AD) dramatically enhances the localization of Bcr-Abl on the vesicle-like structures. These distinct localization patterns of Bcr-Abl and Bcr-Abl-AD enabled us to examine the localization of Bcr-Abl substrate and interacting proteins in relation to Bcr-Abl. We found that a subset of biochemically defined target proteins of Bcr-Abl redistributed and co-localized with Bcr-Abl on F-actin and on vesicle-like structures. The co-localization of signaling proteins with Bcr-Abl at its sites of localization supports the idea that Bcr-Abl forms a multi-protein signaling complex, while the polarized distribution and vesicle-like localization of Bcr-Abl may play a role in leukemogenesis. (+info)Expression of the naturally occurring truncated trkB neurotrophin receptor induces outgrowth of filopodia and processes in neuroblastoma cells. (3/10733)
We have investigated the effects of the truncated trkB receptor isoform T1 (trkB.T1) by transient transfection into mouse N2a neuroblastoma cells. We observed that expression of trkB.T1 leads to a striking change in cell morphology characterized by outgrowth of filopodia and processes. A similar morphological response was also observed in SH-SY5Y human neuroblastoma cells and NIH3T3 fibroblasts transfected with trkB.T1. N2a cells lack endogenous expression of trkB isoforms, but express barely detectable amounts of its ligands, brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT-4). The morphological change was ligand-independent, since addition of exogenous BDNF or NT-4 or blockade of endogenous trkB ligands did not influence this response. Filopodia and process outgrowth was significantly suppressed when full-length trkB.TK+ was cotransfected together with trkB.T1 and this inhibitory effect was blocked by tyrosine kinase inhibitor K252a. Transfection of trkB.T1 deletion mutants showed that the morphological response is dependent on the extracellular, but not the intracellular domain of the receptor. Our results suggest a novel ligand-independent role for truncated trkB in the regulation of cellular morphology. (+info)Plasma membrane recruitment of RalGDS is critical for Ras-dependent Ral activation. (4/10733)
In COS cells, Ral GDP dissociation stimulator (RalGDS)-induced Ral activation was stimulated by RasG12V or a Rap1/Ras chimera in which the N-terminal region of Rap1 was ligated to the C-terminal region of Ras but not by Rap1G12V or a Ras/Rap1 chimera in which the N-terminal region of Ras was ligated to the C-terminal region of Rap1, although RalGDS interacted with these small GTP-binding proteins. When RasG12V, Ral and the Rap1/Ras chimera were individually expressed in NIH3T3 cells, they localized to the plasma membrane. Rap1Q63E and the Ras/Rap1 chimera were detected in the perinuclear region. When RalGDS was expressed alone, it was abundant in the cytoplasm. When coexpressed with RasG12V or the Rap1/Ras chimera, RalGDS was detected at the plasma membrane, whereas when coexpressed with Rap1Q63E or the Ras/Rap1 chimera, RalGDS was observed in the perinuclear region. RalGDS which was targeted to the plasma membrane by the addition of Ras farnesylation site (RalGDS-CAAX) activated Ral in the absence of RasG12V. Although RalGDS did not stimulate the dissociation of GDP from Ral in the absence of the GTP-bound form of Ras in a reconstitution assay using the liposomes, RalGDS-CAAX could stimulate it without Ras. RasG12V activated Raf-1 when they were coexpressed in Sf9 cells, whereas RasG12V did not affect the RalGDS activity. These results indicate that Ras recruits RalGDS to the plasma membrane and that the translocated RalGDS induces the activation of Ral, but that Rap1 does not activate Ral due to distinct subcellular localization. (+info)Id helix-loop-helix proteins inhibit nucleoprotein complex formation by the TCF ETS-domain transcription factors. (5/10733)
The Id subfamily of helix-loop-helix (HLH) proteins plays a fundamental role in the regulation of cellular proliferation and differentiation. Id proteins are thought to inhibit differentiation mainly through interaction with other HLH proteins and by blocking their DNA-binding activity. Members of the ternary complex factor (TCF) subfamily of ETS-domain proteins have key functions in regulating immediate-early gene expression in response to mitogenic stimulation. TCFs form DNA-bound complexes with the serum response factor (SRF) and are direct targets of MAP kinase (MAPK) signal transduction cascades. In this study we demonstrate functional interactions between Id proteins and TCFs. Ids bind to the ETS DNA-binding domain and disrupt the formation of DNA-bound complexes between TCFs and SRF on the c-fos serum response element (SRE). Inhibition occurs by disrupting protein-DNA interactions with the TCF component of this complex. In vivo, the Id proteins cause down-regulation of the transcriptional activity mediated by the TCFs and thereby block MAPK signalling to SREs. Therefore, our results demonstrate a novel facet of Id function in the coordination of mitogenic signalling and cell cycle entry. (+info)A cytomegalovirus glycoprotein re-routes MHC class I complexes to lysosomes for degradation. (6/10733)
Mouse cytomegalovirus (MCMV) early gene expression interferes with the major histocompatibility complex class I (MHC class I) pathway of antigen presentation. Here we identify a 48 kDa type I transmembrane glycoprotein encoded by the MCMV early gene m06, which tightly binds to properly folded beta2-microglobulin (beta2m)-associated MHC class I molecules in the endoplasmic reticulum (ER). This association is mediated by the lumenal/transmembrane part of the protein. gp48-MHC class I complexes are transported out of the ER, pass the Golgi, but instead of being expressed on the cell surface, they are redirected to the endocytic route and rapidly degraded in a Lamp-1(+) compartment. As a result, m06-expressing cells are impaired in presenting antigenic peptides to CD8(+) T cells. The cytoplasmic tail of gp48 contains two di-leucine motifs. Mutation of the membrane-proximal di-leucine motif of gp48 restored surface expression of MHC class I, while mutation of the distal one had no effect. The results establish a novel viral mechanism for downregulation of MHC class I molecules by directly binding surface-destined MHC complexes and exploiting the cellular di-leucine sorting machinery for lysosomal degradation. (+info)Activation of c-Jun N-terminal kinase 1 by UV irradiation is inhibited by wortmannin without affecting c-iun expression. (7/10733)
Activation of c-Jun N-terminal kinases (JNKs)/stress-activated protein kinases is an early response of cells upon exposure to DNA-damaging agents. JNK-mediated phosphorylation of c-Jun is currently understood to stimulate the transactivating potency of AP-1 (e.g., c-Jun/c-Fos; c-Jun/ATF-2), thereby increasing the expression of AP-1 target genes. Here we show that stimulation of JNK1 activity is not a general early response of cells exposed to genotoxic agents. Treatment of NIH 3T3 cells with UV light (UV-C) as well as with methyl methanesulfonate (MMS) caused activation of JNK1 and an increase in c-Jun protein and AP-1 binding activity, whereas antineoplastic drugs such as mafosfamide, mitomycin C, N-hydroxyethyl-N-chloroethylnitrosourea, and treosulfan did not elicit this response. The phosphatidylinositol 3-kinase inhibitor wortmannin specifically blocked the UV-stimulated activation of JNK1 but did not affect UV-driven activation of extracellular regulated kinase 2 (ERK2). To investigate the significance of JNK1 for transactivation of c-jun, we analyzed the effect of UV irradiation on c-jun expression under conditions of wortmannin-mediated inhibition of UV-induced stimulation of JNK1. Neither the UV-induced increase in c-jun mRNA, c-Jun protein, and AP-1 binding nor the activation of the collagenase and c-jun promoters was affected by wortmannin. In contrast, the mitogen-activated protein kinase/ERK kinase inhibitor PD98056, which blocked ERK2 but not JNK1 activation by UV irradiation, impaired UV-driven c-Jun protein induction and AP-1 binding. Based on the data, we suggest that JNK1 stimulation is not essential for transactivation of c-jun after UV exposure, whereas activation of ERK2 is required for UV-induced signaling leading to elevated c-jun expression. (+info)Cell growth inhibition by farnesyltransferase inhibitors is mediated by gain of geranylgeranylated RhoB. (8/10733)
Recent results have shown that the ability of farnesyltransferase inhibitors (FTIs) to inhibit malignant cell transformation and Ras prenylation can be separated. We proposed previously that farnesylated Rho proteins are important targets for alternation by FTIs, based on studies of RhoB (the FTI-Rho hypothesis). Cells treated with FTIs exhibit a loss of farnesylated RhoB but a gain of geranylgeranylated RhoB (RhoB-GG), which is associated with loss of growth-promoting activity. In this study, we tested whether the gain of RhoB-GG elicited by FTI treatment was sufficient to mediate FTI-induced cell growth inhibition. In support of this hypothesis, when expressed in Ras-transformed cells RhoB-GG induced phenotypic reversion, cell growth inhibition, and activation of the cell cycle kinase inhibitor p21WAF1. RhoB-GG did not affect the phenotype or growth of normal cells. These effects were similar to FTI treatment insofar as they were all induced in transformed cells but not in normal cells. RhoB-GG did not promote anoikis of Ras-transformed cells, implying that this response to FTIs involves loss-of-function effects. Our findings corroborate the FTI-Rho hypothesis and demonstrate that gain-of-function effects on Rho are part of the drug mechanism. Gain of RhoB-GG may explain how FTIs inhibit the growth of human tumor cells that lack Ras mutations. (+info)
3T3 cells
... are several cell lines of mouse embryonic fibroblasts. The original 3T3 cell line (3T3-Swiss albino) was established ... 3T3' cells. Since then, several cell lines have been established with this procotol: 3T3-Swiss albino, the original 1962 cell ... "Sublines of mouse 3T3 cells that accumulate lipid". Cell. 1 (3): 113-116. doi:10.1016/0092-8674(74)90126-3. NIH 3T3 Cell Line ... These cells are also contact inhibited. The cells are sensitive to sarcoma virus and leukemia virus focus formation. 3T3 cells ...
3T3-L1
... is a cell line derived from (mouse) 3T3 cells that is used in biological research on adipose tissue. 3T3-L1 cells have a ... "3T3-L1 Cell Line". Retrieved 22 July 2018. Green H, Kehinde O (1975). "An established preadipose cell line and its ... Cellosaurus entry for 3T3-L1 v t e (Rodent cell lines, All stub articles, Cell biology stubs). ... 3T3-L1 cells of the adipocyte morphology increase the synthesis and accumulation of triglycerides and acquire the signet ring ...
Alternatives to animal testing
... detects the viability of 3T3 cells after exposure to a chemical in the presence or absence of light. The 3T3 cell line was ... Cell culture can be an alternative to animal use in some cases. For example, cultured cells have been developed to create ... Tumoroids-3D cell cultures derived from cells biopsied from human patients-can be used in studying the genomics and drug ... "NIH3T3 General Information". NIH 3T3 Cell Line. Retrieved 12 March 2021. Kristian Björnstad; Anders Helander; Peter Hultén; ...
Phoslactomycin B
Effects on the Cytoskeleton in NIH/3T3 Cells". The Journal of Biochemistry. 125 (5): 960-965. doi:10.1093/oxfordjournals.jbchem ... growth factor of the cell such as to induce the formation of mitogen-activated protein interaction and playing a role in cell ...
PEPD
Expression of the mutant enzyme in NIH 3T3 cells". The Journal of Clinical Investigation. 86 (1): 351-5. doi:10.1172/JCI114708 ... Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell. 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. ... doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131. "Infection and Immunity Immunophenotyping (3i) Consortium". Tanoue ... July 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2 ...
Sodium pyruvate
"Exogenous Sodium Pyruvate Stimulates Adipogenesis of 3T3-L1 Cells". Journal of Cellular Biochemistry. 117 (1): 39-48. doi: ... In the body, one way in which sodium pyruvate provides energy to cells is through pyruvate conversion to acetyl-CoA which then ... It is commonly added to cell culture media as an additional source of energy, but may also have protective effects against ... sodium pyruvate has been used in many experiments involving cell cultures to provide more energy. In adipocytes it was found ...
ERBB4
Cohen BD, Green JM, Foy L, Fell HP (Mar 1996). "HER4-mediated biological and biochemical properties in NIH 3T3 cells. Evidence ... Cell. 127 (1): 185-97. doi:10.1016/j.cell.2006.07.037. PMID 17018285. S2CID 18779079. "Entrez Gene: ERBB4 v-erb-a ... Culouscou JM, Plowman GD, Carlton GW, Green JM, Shoyab M (Sep 1993). "Characterization of a breast cancer cell differentiation ... Carpenter G (Mar 2003). "ErbB-4: mechanism of action and biology". Experimental Cell Research. 284 (1): 66-77. doi:10.1016/ ...
Mouse embryonic fibroblast
These cells developed into the commonly used cell line NIH 3T3. MEFs treated by mitomycin or gamma rays (such treatment makes ... Finally digest the remains by enzymes to obtain single isolated cells and culture the cells in a tissue culture dishes. MEF ... Cell. 126 (4): 663-676. doi:10.1016/j.cell.2006.07.024. hdl:2433/159777. PMID 16904174. (All articles with dead external links ... "Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines". J. Cell Biol ...
Adipogenesis
In vitro studies on differentiation have used the pre-committed preadipocyte lineage, such as 3T3-L1 and 3T3-F442A cell line, ... Green H, Kehinde O (28 February 1974). "Sublines of mouse 3T3 cells that accumulate lipid". Cell. 1 (3): 113-116. doi:10.1016/ ... PPARγ is necessary and sufficient to promote fat cell differentiation. PPARγ is required for embryonic stem cells (ES cells) ... "Spontaneous heritable changes leading to increased adipose conversion in 3T3 cells". Cell. 7 (1): 105-13. doi:10.1016/0092-8674 ...
Fibroblast growth factor
Armelin HA (Sep 1973). "Pituitary extracts and steroid hormones in the control of 3T3 cell growth". Proceedings of the National ... For example: FGF15 and FGF19 (FGF15/19) are produced by intestinal cells but act on FGFR4-expressing liver cells to ... "Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth". Nature. 249 (453 ... While many FGFs can be secreted by cells to act on distant targets, some FGF act locally within a tissue, and even within a ...
Infections associated with diseases
Fusinski, Keith A (2008). Adenovirus 36 E4orf1 gene induces differentiation of 3T3-L1 cells (PhD Dissertation). Wayne State ... Cell. 141 (7): 1135-45. doi:10.1016/j.cell.2010.05.009. PMC 2908380. PMID 20602997. Gavranich, John B; Chang, Anne B (2015). " ... and Merkel cell polyomavirus (MCPyV) in non small cell lung cancer". Experimental and Molecular Pathology. 89 (3): 222-6. doi: ... February 2014). "Coxsackievirus B1 is associated with induction of β-cell autoimmunity that portends type 1 diabetes". Diabetes ...
MAN2A1
Moremen KW, Touster O (Jun 1985). "Biosynthesis and modification of Golgi mannosidase II in HeLa and 3T3 cells". The Journal of ... Velasco A, Hendricks L, Moremen KW, Tulsiani DR, Touster O, Farquhar MG (Jul 1993). "Cell type-dependent variations in the ... The Journal of Cell Biology. 115 (6): 1521-34. doi:10.1083/jcb.115.6.1521. PMC 2289207. PMID 1757461. Oka T, Ungar D, Hughson ... Molecular Biology of the Cell. 15 (5): 2423-35. doi:10.1091/mbc.E03-09-0699. PMC 404034. PMID 15004235. "Entrez Gene: MAN2A1 ...
GPR84
explored 3T3-L1 adipocytes cocultured with RAW264.7 cells to examine this potential interaction. RAW264.7 coculture increases ... There was also a GPR84 downregulation in dentritic cell derived from FcRgamma chain KO mice. In microglial cells, the GPR84 ... EST clones corresponding to hgpr84 were from B cells (leukemia), neuroendocrine lung as well as in microglial cells and ... Ly6G + MDSCs in Lal-/- mice show strong immunosuppression on T cells, which contributes to impaired T cell proliferation and ...
Vitisin A (stilbenoid)
"Vitisin a inhibits adipocyte differentiation through cell cycle arrest in 3T3-L1 cells". Biochemical and Biophysical Research ... and NF-κB activation in RAW 264.7 cells". International Immunopharmacology. 9 (3): 319-323. doi:10.1016/j.intimp.2008.12.005. ...
C-Raf
Kolch W, Heidecker G, Lloyd P, Rapp UR (January 1991). "Raf-1 protein kinase is required for growth of induced NIH/3T3 cells". ... Romero F, Martínez-A C, Camonis J, Rebollo A (June 1999). "Aiolos transcription factor controls cell death in T cells by ... cell migration, inhibition of apoptosis, and cell differentiation. Hereditary gain-of-function mutations of c-Raf are ... "X-linked and cellular IAPs modulate the stability of C-RAF kinase and cell motility". Nat. Cell Biol. 10 (12): 1447-55. doi: ...
Cytosol
"Hindered diffusion of inert tracer particles in the cytoplasm of mouse 3T3 cells". Proc. Natl. Acad. Sci. U.S.A. 84 (14): 4910- ... Examples of these processes include signal transduction from the cell membrane to sites within the cell, such as the cell ... cell signaling, and the generation of action potentials in excitable cells such as endocrine, nerve and muscle cells. The ... without damaging the other cell membranes, only about one quarter of cell protein was released. These cells were also able to ...
Ronald Vale
"Purified plasma membranes inhibit polypeptide growth factor-induced DNA synthesis in subconfluent 3T3 cells". Journal of Cell ... Cell. 126 (2): 335-348. doi:10.1016/j.cell.2006.05.046. PMC 2851639. PMID 16873064. Carter, Andrew P.; Garbarino, Joan E.; ... He was the president of the American Society for Cell Biology in 2012. He has also been an investigator at the Howard Hughes ... Ronald David Vale (born 1959) is a biochemist and cell biologist. He is a professor at the Department of Cellular and Molecular ...
CEBPA
"Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells". Genes Dev. 5 (9): 1538-52. doi:10.1101 ... Cell. 8 (4): 817-28. doi:10.1016/S1097-2765(01)00366-5. PMID 11684017. Ho PA, Alonzo TA, Gerbing RB, Pollard J, Stirewalt DL, ... The encoded protein is a key regulator of adipogenesis (the process of forming new fat cells) and the accumulation of lipids in ... Wang H, Iakova P, Wilde M, Welm A, Goode T, Roesler WJ, Timchenko NA (October 2001). "C/EBPalpha arrests cell proliferation ...
CEBPB
Cao Z, Umek RM, McKnight SL (Oct 1991). "Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells ... Cell. Biol. 18 (10): 5880-7. doi:10.1128/mcb.18.10.5880. PMC 109174. PMID 9742105. Zhang F, Lin M, Abidi P, Thiel G, Liu J ( ... Cell. 4 (5): 735-43. doi:10.1016/s1097-2765(00)80384-6. PMID 10619021. Liu YW, Tseng HP, Chen LC, Chen BK, Chang WC (July 2003 ... Cell. Biol. 23 (12): 4066-82. doi:10.1128/mcb.23.12.4066-4082.2003. PMC 156132. PMID 12773552. Mo X, Kowenz-Leutz E, Xu H, ...
Induced stem cells
While initial studies revealed that co-culturing epithelial cells with Swiss 3T3 cells J2 was essential for CRC induction, with ... May 2013). "TAP-deficient human iPS cell-derived myeloid cell lines as unlimited cell source for dendritic cell-like antigen- ... June 2014). "Human somatic cell nuclear transfer using adult cells". Cell Stem Cell. 14 (6): 777-80. doi:10.1016/j.stem.2014.03 ... June 2013). "Human embryonic stem cells derived by somatic cell nuclear transfer". Cell. 153 (6): 1228-38. doi:10.1016/j.cell. ...
CEBPD
Cao Z, Umek RM, McKnight SL (Sep 1991). "Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells ... CEBPD is involved in regulation of apoptosis and cell proliferation. It probably acts as tumor suppressor. One study in mice ... Cell Biology. 29 (12): 1525-39. doi:10.1016/S1357-2725(97)00083-6. PMID 9570146. Zhu Y, Saunders MA, Yeh H, Deng WG, Wu KK (Mar ... Cytogenetics and Cell Genetics. 70 (3-4): 188-91. doi:10.1159/000134030. PMID 7789168. Cleutjens CB, van Eekelen CC, van Dekken ...
Cyclin D1
"Requirement for ras proto-oncogene function during serum-stimulated growth of NIH 3T3 cells". Nature. 313 (5999): 241-3. ... Cyclin D1 is expressed in all adult human tissues with the exception of cells derived from bone marrow stem cell lines (both ... Amplification of the CCND1 gene is present in: non-small cell lung cancers (30-46%) head and neck squamous cell carcinomas (30- ... Chromosomal translocation around the cyclin D1 gene locus is often seen in B mantle cell lymphoma. In mantle cell lymphoma, ...
MDM4
"Entrez Gene: MDM4 Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse)". Strachan GD, Jordan-Sciutto KL, ... regulates p53-induced growth arrest and neuronal cell death during early embryonic mouse development". Mol. Cell. Biol. 22 (15 ... Cell. Biol. 22 (21): 7562-71. doi:10.1128/MCB.22.21.7562-7571.2002. PMC 135668. PMID 12370303. Badciong JC, Haas AL (2003). " ... Sabbatini P, McCormick F (2002). "MDMX inhibits the p300/CBP-mediated acetylation of p53". DNA Cell Biol. 21 (7): 519-25. doi: ...
Alpha-actinin-1
"Modulation of alpha-actinin levels affects cell motility and confers tumorigenicity on 3T3 cells". J. Cell Sci. 107 (7): 1773- ... "Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin". J. Cell Biol. 130 (1): 67 ... and alpha-actinin-binding protein localized at cell-cell adherens junctions". J. Biol. Chem. 278 (6): 4103-11. doi:10.1074/jbc. ... Cell. 10 (4): 819-32. doi:10.1091/mbc.10.4.819. PMC 25204. PMID 10198040. Vallenius T, Luukko K, Mäkelä TP (April 2000). "CLP- ...
Bone morphogenetic protein 4
"Bone morphogenetic protein-2 causes commitment and differentiation in C3H10T1/2 and 3T3 cells". Growth Factors. 9 (1): 57-71. ... "Regeneration of Thyroid Function by Transplantation of Differentiated Pluripotent Stem Cells". Cell Stem Cell. 17 (5): 527-42. ... If these cells also receive signals from FGF, they will differentiate into the spinal cord; in the absence of FGF the cells ... Also in conjunction with FGF2 it can produce progenitor thyroid cells from pluripotent stem cells in mice and humans. BMP4 has ...
CCAAT-enhancer-binding proteins
Cao Z, Umek RM, McKnight SL (Sep 1991). "Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells ... C/EBPβ plays a role in neuronal differentiation, in learning, in memory processes, in glial and neuronal cell functions, and in ... In contrast, ectopic expression of C/EBPβ and δ in 3T3-L1 preadipocytes promotes adipogenesis, even in the absence of ... These proteins are found in hepatocytes, adipocytes, hematopoietic cells, spleen, kidney, brain, and many other organs. C/EBP ...
1,1,2,2-Tetrachloroethane
Enhancement of BALB/c 3T3 cells transformation by 1,2- dibromoethane promoting effect. Carcinogenesis 17(2):225-231. Story DL, ... In 1962, a study showed that 2⁄3 of the investigated rats exposed to 9000 ppm TeCA for 29 days had decreased red blood cells ... Workers in an artificial silk factory that had regularly inhaled TeCA, showed elevated white blood cell levels and slight ...
Victimless Leather
... coated it with 3T3 mouse cells to form connective tissue and topped it up with human bone cells in order to create a stronger ... The 3T3 mouse cells all come from a mouse who lived in the 1970s. The research and development of "Victimless Leather" has been ... To idealize the "victimless" of the jacket, immortalized cell lines or cells that divide and multiply forever once they are ... Victimless Leather - a prototype of a stitch-less jacket, grown from cell cultures into a layer of tissue supported by a coat ...
Ravi Iyengar
Ram, P. T.; Horvath, C. M.; Iyengar, R (2000). "Stat3-mediated transformation of NIH-3T3 cells by the constitutively active ... His laboratory focuses on how cell signals are routed and processed through cellular signaling networks within cells to ... "Stat3-mediated transformation of NIH-3T3 cells by the constitutively active Q205L Galphao protein". Science. 287 (5450): 142-4 ... Cell. 133 (4): 666-80. doi:10.1016/j.cell.2008.04.025. PMC 2728678. PMID 18485874. Bhalla, U. S.; Iyengar, R (1999). "Emergent ...
Melanocortin 2 receptor accessory protein
"Identification of novel putative membrane proteins selectively expressed during adipose conversion of 3T3-L1 cells". ... and also on the cell membrane, which indicates that MC2 needs MRAP to reach the cell membrane. In addition to cell trafficking ... MRAP mutations were found to disable the movement of MC2 to the cell surface of adrenal gland cells; this would make MC2 ... In fat cells, where MC2 is expressed, MRAP was found to facilitate MC2 activated lipolysis and therefore regulating energy ...
NOL1
Overexpression of p120 leads to malignant transformation of 3T3 cells while treatment with antisense p120 mRNA causes the ... "Increased growth of NIH/3T3 cells by transfection with human p120 complementary DNA and inhibition by a p120 antisense ... Cell. 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. v t e (Genes on human chromosome 12, All ... Cell Biol. 4 (7): 529-33. doi:10.1038/ncb814. PMID 12080348. S2CID 24923289. Holsinger LJ, Ward K, Duffield B, Zachwieja J, ...
Syntaxin 3
"Interaction of Munc-18-2 with syntaxin 3 controls the association of apical SNAREs in epithelial cells". J. Cell Sci. 111 (17 ... Tellam JT, Macaulay SL, McIntosh S, Hewish DR, Ward CW, James DE (1997). "Characterization of Munc-18c and syntaxin-4 in 3T3-L1 ... "Human syntaxin 3 is localized apically in human intestinal cells". J. Cell Sci. 110 (18): 2207-14. doi:10.1242/jcs.110.18.2207 ... Cell. 9 (6): 1437-48. doi:10.1091/mbc.9.6.1437. PMC 25366. PMID 9614185. Riento K, Galli T, Jansson S, Ehnholm C, Lehtonen E, ...
Dock11
... is expressed at lower levels in NIH-3T3 fibroblasts, C2C12 myoblasts and Neuro-2A neuroblastoma cells. Dock11 mRNA has ... Côté JF, Vuori K (August 2007). "GEF what? Dock180 and related proteins help Rac to polarize cells in new ways". Trends in Cell ... Meller N, Merlot S, Guda C (November 2005). "CZH proteins: a new family of Rho-GEFs". Journal of Cell Science. 118 (Pt 21): ... Cell Cycle. 6 (6): 750-7. doi:10.4161/cc.6.6.3986. PMC 2040307. PMID 17361101. Lin Q, Yang W, Baird D, Feng Q, Cerione RA ( ...
Androgen
"Testosterone inhibits adipogenic differentiation in 3T3-L1 cells: nuclear translocation of androgen receptor complex with beta- ... These are Leydig cells. Soon after they differentiate, Leydig cells begin to produce androgens. The androgens function as ... The mesoderm-derived epithelial cells of the sex cords in developing testes become the Sertoli cells, which will function to ... Androgens promote the enlargement of skeletal muscle cells in a coordinated manner by acting on several cell types in skeletal ...
HSPA8
... factor-1 and the heat shock cognate 70 protein associate in high molecular weight complexes in the cytoplasm of NIH-3T3 cells ... Cell. 122 (6): 957-68. doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070. S2CID 8235923. Imai Y ... an apoptotic cell undergoes structural changes including cell shrinkage, plasma membrane blebbing, nuclear condensation, and ... "Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells". Cell Structure ...
Ludwigia octovalvis
... system and AMPK pathway in 3T3-L1 cells". Food Chem. Toxicol. 48 (2): 716-21. doi:10.1016/j.fct.2009.12.001. PMID 19963029. Lin ...
PIKFYVE
... inhibitors cause cell death also in A-375 melanoma cells, which depend on autophagy for growth and proliferation, due ... "ArPIKfyve-PIKfyve interaction and role in insulin-regulated GLUT4 translocation and glucose transport in 3T3-L1 adipocytes". ... Cell. 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. (Genes on human chromosome 2). ... "PIKfyve regulates CaV1.2 degradation and prevents excitotoxic cell death". The Journal of Cell Biology. 187 (2): 279-94. doi: ...
Alan Hall
DNA from a rhabdomyosarcoma cell line and a fibrosarcoma cell line transformed a NIH/3T3 mouse fibroblast cell line. After ... He first realised that the addition of bovine fetal calf serum (FCS) to Swiss 3T3 cells increased the polymerisation of actin ... "Prominent Cell Biologist Dies". In Memoriam: Alan Hall, Pioneer in Rho GTPases and Chair of Cell Biology at Sloan-Kettering "At ... cells. Downregulation of RhoA in the HBE cell lines using siRNAs showed a lack of apical junction formation in contrast with ...
Simpson-Golabi-Behmel syndrome
These cells are similar to human primary preadipocytes, and may or may not become a popular model instead of Mouse 3T3-L1 cells ... It has been suggested that SGBS type II may be caused by duplication of the GPC4 gene, which helps to regulate cell division ... The function of this gene is to produce a protein that acts as a cell surface receptor that binds to transcription factors. ... SGBS Cells are a unique tool to study the function of Human adipocyte biology. ...
Avicularin
It suppresses lipid accumulation through repression of C/EBPα-activated GLUT4-mediated glucose uptake in 3T3-L1 cells. LC ... suppresses lipid accumulation through repression of C/EBPα-activated GLUT4-mediated glucose uptake in 3T3-L1 cells. Fujimori K ...
VAMP2
Journal of Cell Science. 112. 112 (6): 845-54. doi:10.1242/jcs.112.6.845. PMID 10036234. Margittai M, Otto H, Jahn R (Mar 1999 ... "Role of SNAP23 in insulin-induced translocation of GLUT4 in 3T3-L1 adipocytes. Mediation of complex formation between syntaxin4 ... Cell. 83 (1): 111-9. doi:10.1016/0092-8674(95)90239-2. PMID 7553862. S2CID 675343. Pérez-Brangulí F, Muhaisen A, Blasi J (Jun ... The Journal of Cell Biology. 143 (4): 957-71. doi:10.1083/jcb.143.4.957. PMC 2132958. PMID 9817754. Nishimura Y, Hayashi M, ...
EBF1
"Gene expression analysis suggests that EBF-1 and PPARgamma2 induce adipogenesis of NIH-3T3 cells with similar efficiency and ... EBF1 stands for Early B-Cell Factor 1. EBF1 controls the expression of key proteins required for B cell differentiation, signal ... "Early B-cell factor 1 (EBF1) is critical for transcriptional control of SLAMF1 gene in human B cells". Biochimica et Biophysica ... "Early B Cell Factor 1 Regulates B Cell Gene Networks by Activation, Repression, and Transcription- Independent Poising of ...
Platelet-derived growth factor
... vascular smooth muscle cells and mesenchymal stem cells as well as chemotaxis, the directed migration, of mesenchymal cells. ... Paul D, Lipton A, Klinger I (1971). "Serum factor requirements of normal and simian virus 40-transformed 3T3 mouse fibroplasts ... PDGF is a potent mitogen for cells of mesenchymal origin, including fibroblasts, smooth muscle cells and glial cells. In both ... "Cell Death and Control of Cell Survival in the Oligodendrocyte Lineage". Cell. 70 (1): 31-46. doi:10.1016/0092-8674(92)90531-G ...
Karen Leach
Type 3 protein kinase C localization to the nuclear envelope of phorbol ester-treated NIH 3T3 cells. J. Cell. Biol., 109:685- ... "Type 3 protein kinase C localization to the nuclear envelope of phorbol ester-treated NIH 3T3 cells". The Journal of Cell ... Molecular Cell 26: 393-402, 2007. Nagiec, Eva E.; Wu, Luping; Swaney, Steve M.; Chosay, John G.; Ross, Daniel E.; Brieland, ... By showing mitochondrial protein synthesis was the link to Zyvox cytotoxicity in human cells, this research led to important ...
Macrophage colony-stimulating factor
... and downmodulation of CSF-1 receptors in NIH 3T3 cells transformed by cotransfection of the human CSF-1 and c-fms (CSF-1 ... and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By ... is a secreted cytokine which causes hematopoietic stem cells to differentiate into macrophages or other related cell types. ... Eukaryotic cells also produce M-CSF in order to combat intercellular viral infection. It is one of the three experimentally ...
Taxillus kaempferi
... suppresses lipid accumulation through repression of C/EBPα-activated GLUT4-mediated glucose uptake in 3T3-L1 cells. Fujimori K ...
PDGFRB
In vitro studies using cultured cells indicate that endothelial cells secrete PDGF, which recruits PDGFRβ-expressing pericytes ... Chen M, She H, Kim A, Woodley DT, Li W (November 2000). "Nckbeta adapter regulates actin polymerization in NIH 3T3 fibroblasts ... Olson LE, Soriano P (2011). "PDGFRβ signaling regulates mural cell plasticity and inhibits fat development". Developmental Cell ... Cell. Biol. 12 (12): 5824-33. doi:10.1128/MCB.12.12.5824. PMC 360522. PMID 1333047. Chen M, She H, Davis EM, Spicer CM, Kim L, ...
SNAP23
"Interaction of Munc-18-2 with syntaxin 3 controls the association of apical SNAREs in epithelial cells". Journal of Cell ... "Role of SNAP23 in insulin-induced translocation of GLUT4 in 3T3-L1 adipocytes. Mediation of complex formation between syntaxin4 ... "Relocation of the t-SNARE SNAP-23 from lamellipodia-like cell surface projections regulates compound exocytosis in mast cells ... Synaptobrevin/VAMP and syntaxin are believed to be involved in vesicular transport in most, if not all cells, while SNAP25 is ...
EHD1
doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. v t e (Genes on human chromosome 11, All stub articles, Human ... "Role of EHD1 and EHBP1 in perinuclear sorting and insulin-regulated GLUT4 recycling in 3T3-L1 adipocytes". J. Biol. Chem. 279 ( ... Cell. 15 (5): 2410-22. doi:10.1091/mbc.E03-10-0733. PMC 404033. PMID 15020713. Guilherme A, Soriano NA, Furcinitti PS, Czech MP ... 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635-48. ...
FGD1
It also promotes the transition through G1 during the cell cycle and causes tumorgenic transformation of NIH/3T3 fibroblasts. ... enabling the cells to migrate. FGD1 also activates the c-Jun N-terminal kinase (JNK) signaling cascade, important in cell ... a key player in the establishment of cell polarity in all eukaryotic cells. The GEF activity of FGD1, which activates Cdc42, is ... Cell. 20 (9): 2413-27. doi:10.1091/mbc.E08-11-1136. PMC 2675621. PMID 19261807. Olson MF, Pasteris NG, Gorski JL, Hall A ( ...
List of MeSH codes (A11)
... l cells MeSH A11.329.228.900 - 3t3 cells MeSH A11.329.228.900.080 - balb 3t3 cells MeSH A11.329.228.900.550 - nih 3t3 cells ... cho cells MeSH A11.251.210.505 - l cells (cell line) MeSH A11.251.210.520 - llc-pk1 cells MeSH A11.251.210.700 - 3t3 cells MeSH ... balb 3t3 cells MeSH A11.251.210.700.550 - nih 3t3 cells MeSH A11.251.210.700.775 - Swiss 3t3 cells MeSH A11.251.210.700.775.800 ... Swiss 3t3 cells MeSH A11.329.228.900.775.800 - 3t3-l1 cells MeSH A11.329.228.950 - mesangial cells MeSH A11.329.372.300 - ...
Nuclear protein in testis gene
Studies on cultured immortalized human dermal keratinocyte (i.e. HDK) and mouse embryonic fibroblast NIH-3T3 cell lines found ... Overexpression of the MYC and SOX2 genes can also act to maintain cells in an undifferentiated stem cell-like state similar to ... round cell, and epithelioid cell-like features which are regarded as sarcomas. Sarcomas with NUTM1 fusion genes typically a) ... Genome-Wide H4 Hyperacetylation in Male Germ Cells" (PDF). Cell Reports. 24 (13): 3477-3487.e6. doi:10.1016/j.celrep.2018.08. ...
Canarium indicum
... production in 3T3 Swiss Albino fibroblast cells, which workes in the same ways as aspirin. However, the IgE specific to ...
Mir-872 microRNA precursor family
... miR-872 levels are reduced with inhibition of adipocytes of the 3T3-L1 cell line, along with those of miRNAs-155 and -183. ... 2011). "Loss of Dicer in Sertoli cells has a major impact on the testicular proteome of mice". Mol Cell Proteomics. 10 (4): ... Chang CL, Au LC, Huang SW, Fai Kwok C, Ho LT, Juan CC (2011). "Insulin up-regulates heme oxygenase-1 expression in 3T3-L1 ... miR-872 has been found to be expressed in sertoli cells and to post-transcriptionally target the Sod-1 gene, which encodes the ...
Rho family of GTPases
Virtually all eukaryotic cells form such processes upon Rho activation. Fibroblasts such as Swiss 3T3 cells are often used to ... Before the cell can bud, Cdc42 is used to locate the region of the cell's membrane that will begin to bulge into the new cell. ... Before cells can undergo key processes such as budding, mitosis, or locomotion, it must have some manner of cell polarity. One ... Animal cells form many different shapes based on their function and location in the body. Rho proteins help cells regulate ...
PTGFRN
"Synthesis and accumulation of a receptor regulatory protein associated with lipid droplet accumulation in 3T3-L1 cells". J. ... 2001). "PGRL is a major CD81-associated protein on lymphocytes and distinguishes a new family of cell surface proteins". J. ... 2006). "Contrasting effects of EWI proteins, integrins, and protein palmitoylation on cell surface CD9 organization". J. Biol. ...
N-alpha-acetyltransferase 10
Two alternative splicing products of mouse Naa10, mNaa10235 and mNaa10225, were reported in NIH-3T3 and JB6 cells that may have ... including cell migration, cell cycle control, DNA damage control, caspase-dependent cell death, p53 dependent apoptosis, cell ... Cell. 146 (4): 607-20. doi:10.1016/j.cell.2011.06.050. PMC 3182480. PMID 21854985. Kuo HP, Lee DF, Chen CT, Liu M, Chou CK, Lee ... Whiteway M, Szostak JW (December 1985). "The ARD1 gene of yeast functions in the switch between the mitotic cell cycle and ...
Creator: Nathans, Daniel, 1928-1999 / Subject: BALB 3T3 Cells and Simian virus 40 / Exhibit Tags: enzymes - Daniel Nathans -...
Embryonic Swiss Mouse Fibroblast Cells (3T3) | Olympus LS
Embryonic Swiss Mouse Fibroblast Cells (3T3). Alexa Fluor 568 conjugated to phalloidin was utilized to label a 3T3 cell culture ... Cells in Culture/ 3t3/ Embryonic Swiss Mouse Fibroblast Cells (3T3). ... The cells were simultaneously stained with Alexa Fluor 488 conjugated to concanavalin A, a lectin displaying very high affinity ... for intracellular filamentous actin, while DAPI was used to target DNA in the cell nuclei. ...
Effects of Berberine on Adipose Tissues and Kidney Function in 3T3-L1 Cells and Spontaneously Hypertensive Rats. | Nat Prod...
... as well as its effect on renal injury in 3T3-L1 cells and spontaneously hypertensive rats. 3T3-L1 cells were cultured and ... Effects of Berberine on Adipose Tissues and Kidney Function in 3T3-L1 Cells and Spontaneously Hypertensive Rats. ... Effects of Berberine on Adipose Tissues and Kidney Function in 3T3-L1 Cells and Spontaneou ... and inhibit 3T3-Ll fibroblast differentiation to adipocytes. Male spontaneously hypertensive rats received either 150 mg/day of ...
Calcium content and distribution as a function of growth and transformation in the mouse 3T3 cell - Fingerprint - Experts...
ARCHIVED - Environment and Climate Change Canada - Screening Assessment for the Challenge Naphthalene
Cell transformation assay. Negative: RLV-infected Fischer rat embryo cell; without activation (Freeman et al. 1973); BALB/3T3 ... Criterion development for the application of BALB/c-3T3 cells to routine testing for chemical carcinogenic potential. Res J ... Transformation of cell cultures as an indication of the carcinogenic potential of chemicals. J Natl Cancer Inst 51:799-808 [ ... In vitro prediction of carcinogenicity using a bovine papillomavirus DNA-carrting C3H/10T½ cell line (T1). II: Results from the ...
A novel mouse model for inhibition of DOHH-mediated hypusine modification reveals a crucial function in embryonic development,...
Human Papillomavirus16 Variant E7 Gene Induces Transformation of NIH 3T3 Cells Via Up-Regulation of cdc25A and Cyclin A |...
Human Papillomavirus16 Variant E7 Gene Induces Transformation of NIH 3T3 Cells Via Up-Regulation of cdc25A and Cyclin A ... Human Papillomavirus16 Variant E7 Gene Induces Transformation of NIH 3T3 Cells Via Up-Regulation of cdc25A and Cyclin A ... Human Papillomavirus16 Variant E7 Gene Induces Transformation of NIH 3T3 Cells Via Up-Regulation of cdc25A and Cyclin A ...
Steroid Sulfatase in the Mouse NIH-3T3 Fibroblast Cell Line: Characterization, and Downregulation by Glucocorticoids - Cotinis...
Single cell analyses revealed that the expression of mA3 in NIH 3T3 cells was limited to 20% of the cells, which likely ... NIH-3T3). Using a radioactive estrone sulfate (E1S) conversion assay, we detected STS activity in cultured NIH-3T3 cells. ... and antioxidants depletion in NIH-3T3 cells.. Treatment with naringin (60 µM) prior UVB exposure prevented the cell ... NIH/3T3 BALB Cell Slide (Fibroblast from 14- to 17-day-old BALB/c mouse embryos) (5 slides/pk). from Alpha Diagnostics ...
ArboCat Virus: Parana (PARV)
NIOSHTIC-2 Search Results - Full View
Non-transformed BALB/c-3T3 cells and cells from 10 transformed cell lines induced by CdCl(2) were injected into both axillary ... and the transformed cells induced by CdCl(2) can form tumors in nude mice. BALB/c-3T3 cells were treated with different ... Cell transformation; Gene transfection; Tumorigenicity; Cadmium chloride; BALB/c-3T3 cells ... induced transformed cell lines were isolated and gene transfection assay was performed using NIH-3T3 cells. ...
Ecm gel | Sigma-Aldrich
A proteomic approach to investigate AuNPs effects in Balb/3T3 cells.. Sabrina Gioria. et al. ... Stem cell research & therapy, 13(1), 389-389 (2022-07-31). Induced pluripotent stem cells (iPSCs) have the potential to promote ... Stem cells international, 2020, 9054514-9054514 (2020-07-03). Adipose-derived stem cell- (ADSC-) based regenerative medicine ... Interactions with the extracellular matrix (ECM) play an important role in regulating cell function. Cells cultured in, or on, ...
Isolation of Waddlia malaysiensis, A Novel Intracellular Bacterium, from Fruit Bat (Eonycteris spelaea) - Volume 11, Number 2...
... and B-lymphoblastoid cells lines; and of simian kidney (LLC-MK2) and rodent epithelial (3T3, BHK) cell lines. Figures 1B and 1B ... Total DNA was extracted from a 72-h culture of the bacterium in Vero cells. The infected cells were scraped from a 25-cm2 ... On negative-stain electron microscopy of the supernatant from G817 cultured on Vero cells, small bacterial cells (0.4-0.6 μ) ... To determine the range of cells susceptible to infection, different cells were cultured in 25-m2 plastic flasks (Becton ...
Thrombus Degradation by Fibrinolytic Enzyme of Stenotrophomonas sp. Originated from Indonesian Soybean-Based Fermented Food on...
Blood clot in the tail was induced by kappa carrageenan injection at 1 mg/kg BW.,i, Results,/i,. Experiment with cell ... The effect on expression of tissue plasminogen activator was also analysed in the cell culture. For,i, in vivo ,/i,studies, 25 ... of Indonesia.,i, Methods,/i,. Prior to animal study, the enzyme safety was tested using cell culture. ... 3T3 cell counts after treatment with crude enzyme (a); HeLa S3 cell counts after treatment with crude enzyme; (b) HeLa S3 cell ...
Influenza infection rewires energy metabolism and induces browning features in adipose cells and tissues | Communications...
Importantly, viral RNA- and viral antigen-harboring cells are detected in the WAT of infected mice. Using in vitro approaches, ... reprograms host cells glucose and lipid metabolism to promote its own replication. However, the impact of influenza infection ... The differentiation of 3T3-L1 cells into mature adipocytes was conducted as indicated in ref. 40: 3T3-L1 preadipose cells were ... The mouse 3T3-L1 preadipose cell-line (ATCC®, CL-173TM) was used. 3T3-L1 cells were cultured in Dulbeccos modified Eagles ...
The E3 ubiquitin ligase TRIM23 regulates adipocyte differentiation via stabilization of the adipogenic activator PPARγ | eLife
D) Cell proliferation analysis of 3T3-L1 cells during adipocyte differentiation. 3T3-L1 cells were counted at each time point. ... B) Changes in Trim23 mRNA during adipocyte differentiation in 3T3-L1 cells. Total RNA was isolated from 3T3-L1 cells on the ... Cell proliferation assay. Request a detailed protocol Total cell number per dish was counted during a period of 6 days. 3T3-L1 ... A) Real-time PCR analysis of mRNA expression of Trim23 during 3T3-L1 differentiation. Total RNA was isolated from 3T3-L1 cells ...
CD28 Costimulatory Domain-Targeted Mutations Enhance Chimeric Antigen Receptor T-cell Function | Cancer Immunology Research |...
CAR T cells were stimulated with 3T3-mCD19 target cells at a 10:1 E:T ratio for 24 hours. Cells were sorted using a FACSAria II ... A, B-cell and CAR T-cell counts in the blood in weeks 1 to 6. B, B-cell and CAR T-cell percentage in BM after 8 weeks. C, CD28 ... A, B-cell and CAR T-cell counts in the blood in weeks 1 to 6. B, B-cell and CAR T-cell percentage in BM after 8 weeks. C, CD28 ... To generate 3T3-mPD-L1 target cells, 1 × 105 NIH/3T3 cells (ATCC) were transduced with mouse PD-L1 (pLTC-mPD-L1; G&P ...
Recombinant Human FGF-acidic
Biological Activity: Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is ≤ 0.5 ng/ml in the ... Cell Culture FGF Superfamily Immune System Inflammation Neurobiology Stem Cells & Differentiation Wound Healing ... smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through ... Targeted delivery of regulatory macrophages to lymph nodes interferes with T cell priming by preventing the formation of stable ...
George M. Gray - SourceWatch
Gray, G.M., and Macara, I.G. (1989) Serum-stimulated Phosphatidylinositol Turnover is Enhanced in 3T3 Cells with Active pp60v- ... Macara, I.G., and Gray, G.M. (1987) Vanadate-Activated Calcium Influx in A431 Cells Is Dependent on the Plasma Membrane ... Gray, G.M., and Macara, I.G. (1988) The pp60v-src Tyrosine Kinase Desensitizes Epidermal Growth Factor Binding to 3T3 ... and Vanadate-Activated Calcium Influx in A431 Cells. in Growth Factors, Tumor Promoters, and Cancer Genes, Alan R. Liss, Inc. ...
JCI -
Sustained activation of SMAD3/SMAD4 by FOXM1 promotes TGF-β-dependent cancer metastasis
... and the cells were fused with murine NIH 3T3 cells. NIH 3T3 nuclei that are easily distinguished by the presence of brightly ... MDA-MB-231 cells (1 × 106 cells/mouse) were injected into the lateral tail vein of mice. 4T07 (1 × 105 cells/mouse) or 4T1 (5 ... 24 hours later, the cells were replated together with NIH 3T3 cells. After coculture overnight, 100 μg/ml cycloheximide (Sigma- ... Cell culture and transfection. HEK 293T, MDA-MB-231, BT-474 cells, and immortalized FOXM1 conditional knockout Foxm1fl/fl MEFs ...
Fatty Acid Synthase/FASN Antibody (NB400-114): Novus Biologicals
Andrographolide inhibits adipogenesis of 3T3-L1 cells by suppressing C/EBPbeta expression and activation. Toxicol. Appl. ... FASN was found to localize to the cytoplasm of tumor cells and a representative image is shown in Figure 1F. IHC-P. Human. ... In ICC/IF cytoplasmic staining can be seen in MCF7 cells. The observed molecular weight of the protein may vary from the listed ... Androgen deprivation increases p300 expression in prostate cancer cells. Cancer Res;67(7):3422-30. 2007-04-01 [PMID: 17409453] ...
Microglia LPS-Related Module Antibody Sampler Kit | Cell Signaling Technology
Flow cytometric analysis of NIH/3T3 cells (red) and 32D cells (blue), using HS1 (D5A9) XP® Rabbit mAb.. Show Less Show More ... Flow cytometric analysis of NIH/3T3 cells (red) and 32D cells (blue), using HS1 (D5A9) XP® Rabbit mAb.. ... Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells, untreated or staurosporine-treated (1 µM), and Jurkat cells ... Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells, untreated or staurosporine-treated (1 µM), and Jurkat cells ...
Recombinant Human FGFR1 alpha (IIIb) Fc Chimera Protein, CF 655-FR-050: R&D Systems
Measured by its ability to inhibit FGF acidic-dependent proliferation of NR6R‑3T3 mouse fibroblast cells. The ED50 for this ... Mouse myeloma cell line, NS0-derived human FGF R1 alpha protein. Human FGF R1 alpha (IIIb) (Arg22 - Lys310). Accession # NP_ ... In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. ... FGF2 posttranscriptionally down-regulates expression of SDF1 in bone marrow stromal cells through FGFR1 IIIc. Authors: Nakayama ...
Protective effect of avicularin on rheumatoid arthritis and its associated mechanisms
Fujimori and Shibano (18) indicated that avicularin may suppresses lipid accumulation in mouse adipocytic 3T3-L1 cells. These ... Cell apoptosis assay. MH7A cells were treated with 10, 30, 100 or 300 µM avicularin and 10 ng/ml TNF-α. Then, MH7A cell ... An MTT assay was used to detect cell viability. The MH7A cells were seeded into each well (5×105 cells per well) and incubated ... Effect of avicularin on MEK/nuclear factor kappa light-chain-enhancer of activated B-cells pathway in MH7A cells. MH7A cells ...
Maritime Pine: MedlinePlus Supplements
Mochizuki, M. and Hasegawa, N. Pycnogenol stimulates lipolysis in 3t3-L1 cells via stimulation of beta-receptor mediated ... Cell Mol Life Sci 1998;54:1168-72. View abstract.. *Tixier JM, et al. Evidence by in vivo and in vitro studies that binding of ... Cell Mol Life Sci 2000;57:834-841. View abstract.. * Araghi-Niknam, M., Hosseini, S., Larson, D., Rohdewald, P., and Watson, R ... Bito T, Roy S, Sen CK, Packer L. Pine bark extract pycnogenol downregulates IFN-gamma-induced adhesion of T cells to human ...
The Heterogeneity of White Adipose Tissue | IntechOpen
... and microRNAs that affect the function of cells and tissues throughout the body. Accumulation of visceral white adipose tissue ... Cell Metabolism. 2009;. 9. (3):287-302. *211. Ling HY et al. MicroRNA-375 promotes 3T3-L1 adipocyte differentiation through ... Adipocyte lineage cells contribute to the skin stem cell niche to drive hair cycling. Cell. 2011;. 146. (5):761-771. ... anti-inflammatory cells (i.e. M2 macrophages, regulatory T cells (Treg), and T helper type 2 cells (Th2)) also have defined ...
Biomolecules | Free Full-Text | Bio-Based Formulations for Sustainable Applications in Agri-Food-Pharma
This study revealed that 6-Shogaol to inhibit the growth of both cell types without affecting the normal cell lines (normal 3T3 ... human hepatocellular carcinoma cells, B16 4A5: murine melanoma cells, and S17: murine bone marrow (normal) cells. Bio- ... which caused tumoral cell death, but without any cytotoxic effects on human healthy breast cells. The authors proposed this to ... investigated the anticancer properties of 6-Shogaol against two different human liposarcoma cell lines (SW872 and 93T449 cells ...
Plus it
Utilization of Newly Developed Mouse Monoclonal Anti-Acetyl Lysine Antibodies in Immunofluorescence Applications
Anti-Acetyl Lysine Monoclonal Antibodies are useful to detect and measure acetylated proteins throughout the cell, including ... Cell Culture and Reagents. Swiss 3T3 and A431 cells were grown in DMEM media (ATCC, VA) supplemented with 10% FBS (Altas ... Cells were seeded on glass coverslips for at least 24 hours before treatment. For Swsis 3T3 experiment, cells were either ... Figure 1 Legend: Swiss 3T3 cells were treated with TSA (1 mM for 6 h) and stained as described in the methods. Acetylated ...
BALBFibroblast CellsProliferationAssayTransformationVitroDifferentiationAdipocytesLipidAntibodyHeLa CellsAdipogenesisProteinsRegulationMammalian cellsLineLung cancerActinInduceGenesSubstrateTissueProteinInductionGrowthViabilityConnectiveEnzymesMembranesRegulatoryConcentrationsProtocolMCF7CollagenLinesCultureMiceConfocalSubjectReductionLysosomalHighFoundHealthyExpression
BALB7
- Transforming and carcinogenic potential of cadmium chloride in BALB/c-3T3 cells. (cdc.gov)
- BALB/c-3T3 cells were treated with different concentrations of CdCl(2) for 72 h. (cdc.gov)
- Non-transformed BALB/c-3T3 cells and cells from 10 transformed cell lines induced by CdCl(2) were injected into both axillary regions of nude mice. (cdc.gov)
- Within this time period, no tumors were found in nude mice injected with non-transformed BALB/c-3T3 cells. (cdc.gov)
- A proteomic approach to investigate AuNPs effects in Balb/3T3 cells. (sigmaaldrich.com)
- Determined by a cell proliferation assay using Balb/c 3T3 cells. (peprotech.com)
- Perocco P, Paolini M, Mazzullo M, Biagi GL and Cantelli-Forti G. (1999) -Carotene as enhancer of cell transforming activity of powerful carcinogens and cigarette-smoke condensate on BALB/c 3T3 cells in vitro. (nih.gov)
Fibroblast Cells2
- Measured by its ability to inhibit FGF acidic-dependent proliferation of NR6R‑3T3 mouse fibroblast cells. (rndsystems.com)
- The mouse fibroblast cells undergo an adipose-like conversion as they move to a confluent and contact-inhibited state. (bvsalud.org)
Proliferation5
- Aquaporin 1 is overexpressed in lung cancer and stimulates NIH-3T3 cell proliferation and anchorage-independent growth. (nih.gov)
- Forced expression of full-length AQP1 cDNA in NIH-3T3 cells induced many phenotypic changes characteristic of transformation, including cell proliferation-enhancing activity by the MTT assay and anchorage-independent growth in soft agar. (nih.gov)
- At the cellular level, we observed reduced proliferation and induction of senescence in 3T3 Dohh-/- cells as well as reduced capability for malignant transformation. (nih.gov)
- Finally, in ferrets given high dose beta-carotene supplements and exposed to smoke, there also were elevated expressions of C-jun and C-fos genes, which are involved in cell proliferation. (nih.gov)
- Effects of physiological versus pharmacological -carotene supplementation on cell proliferation and histopathological changes in the lungs of cigarette smoke-exposed ferrets. (nih.gov)
Assay5
- The NRU cytotoxicity assay procedure is a cell survival/viability chemosensitivity assay based on the ability of viable cells to incorporate and bind neutral red (NR), a supravital dye. (nih.gov)
- Such changes brought about by the action of xenobiotics result in a decreased uptake and binding of NR. It is thus possible to distinguish between viable, damaged, or dead cells, which is the basis of this assay. (nih.gov)
- Using a radioactive estrone sulfate (E 1 S) conversion assay, we detected STS activity in cultured NIH-3T3 cells. (cotinis.com)
- DNAs from five CdCl(2)-induced transformed cell lines were isolated and gene transfection assay was performed using NIH-3T3 cells. (cdc.gov)
- Cell viability and apoptosis were detected using an MTT assay and flow cytometry, respectively. (spandidos-publications.com)
Transformation4
- Studies have been performed to determine if cadmium chloride (CdCl(2)) can induce morphological cell transformation, DNA from CdCl(2)-induced transformed cells can transform other mammalian cells, and the transformed cells induced by CdCl(2) can form tumors in nude mice. (cdc.gov)
- DNA from all five CdCl(2)-induced transformed cell lines tested was found to induce varying degrees of transfection-mediated transformation in NIH-3T3 cells. (cdc.gov)
- These results indicate that CdCl(2) is capable of inducing morphological cell transformation and that the transformed cells induced by CdCl(2) are potentially tumorigenic. (cdc.gov)
- The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION . (nih.gov)
Vitro4
- The 3T3 NRU test will be performed to analyze the in vitro toxicity of 60 blinded/coded test chemicals. (nih.gov)
- Despite a growing need for well-understood models for evaluating adipogenic and potential obesogenic contaminants, there has been a reliance on decades-old in vitro models that have not been appropriately managed by cell line providers. (nih.gov)
- Tetraarsenic oxide-induced inhibition of malignant glioma cell invasion in vitro via a decrease in matrix metalloproteinase secretion and protein kinase B phosphorylation. (sigmaaldrich.com)
- To perform this investigation, an in vitro model of RA was established by treatment of the human RA synovial MH7A cell line with tumor necrosis factor‑α (TNF‑α). (spandidos-publications.com)
Differentiation2
- Multilayered dense collagen-silk fibroin hybrid: a platform for mesenchymal stem cell differentiation towards chondrogenic and osteogenic lineages. (sigmaaldrich.com)
- Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. (rndsystems.com)
Adipocytes3
- The 3T3-L1 cell line is one of the most well-characterized and reliable models for studying adipocytes . (bvsalud.org)
- We confirmed that berberine markedly inhibited fat accumulation and lipid droplets in 3T3-L1 adipocytes and decreased triglyceride content. (bvsalud.org)
- Fat cells, also known as adipocytes, arise from precursor cells via a process called adipogenesis. (elifesciences.org)
Lipid1
- Cells were co-stained with Hoechst (nuclei) and Nile red (lipid). (nih.gov)
Antibody1
- Immunocytochemistry/ Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NB400-114] - The Fas antibody was tested in MCF-7 cells at a 1:2000 dilution against Dylight 488 (Green). (novusbio.com)
HeLa Cells3
- Western blot analysis of extracts from control HeLa cells (lane 1) or HeLa cells with an apparent in-frame truncation mutation in the gene encoding LMNA (lane 2) using Lamin A/C (4C11) Mouse mAb #4777 (upper) or α-actinin (D6F6) XP ® Rabbit mAb #6487 (lower). (cellsignal.com)
- The change in LMNA molecular weight in the mutated HeLa cells is consistent with an in-frame deletion. (cellsignal.com)
- Confocal immunofluorescent analysis of HeLa cells using Lamin A/C (4C11) Mouse mAb (green). (cellsignal.com)
Adipogenesis1
- For adipogenesis to proceed, cells have to maintain the appropriate levels of PPARγ. (elifesciences.org)
Proteins3
- Using Franz diffusion cell systems studied the transdermal effect of DSCH and then examined the percutaneous rate and molecular weight distribution of percutaneous proteins (PP). (cotinis.com)
- We have expressed mutant RhoG proteins fused to the green fluorescent protein and analyzed subsequent changes in cell surface morphology and modifications of cytoskeletal structures. (cnrs.fr)
- Adipose tissue not only stores energy, but also controls metabolism through secretion of hormones, cytokines, proteins, and microRNAs that affect the function of cells and tissues throughout the body. (intechopen.com)
Regulation2
- With regard to regulation, treatments of cultured NIH-3T3 cells revealed that cortisol and the synthetic glucocorticoids dexamethasone and prednisolone decreased STS activity, as we have found for cell lines from other tissues. (cotinis.com)
- Our results could have important implications regarding local estrogen production by STS in fibroblasts , which are the most common connective tissue cells in the body, and on possible regulation of local estrogen levels by cortisol. (cotinis.com)
Mammalian cells1
- Healthy mammalian cells, when maintained in culture, continuously divide and multiply over time. (nih.gov)
Line5
- This study was designed to characterize STS activity in a mouse fibroblast cell line (NIH-3T3). (cotinis.com)
- In this study, the application of this system is reported for targeting Rag genes to produce mutant mouse NIH/3T3 cell line. (cotinis.com)
- Flow cytometric analysis of Raw 264.7 cells using IKKε (D61F9) XP ® Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (dashed line). (cellsignal.com)
- 6. Construction of tsc2 knockout cell line using CRISPR/Cas9 system and demonstration of its effects on NIH-3T3 cells. (nih.gov)
- A continuous cell line that is a substrain of SWISS 3T3 CELLS developed though clonal isolation. (bvsalud.org)
Lung cancer1
- Here, we tested 10 non-small cell lung cancer cell lines of various origins by reverse transcriptase-polymerase chain reaction and Western blot analysis and identified clear expression of aquaporin 1 (AQP1) in seven cell lines. (nih.gov)
Actin1
- Alexa Fluor 568 conjugated to phalloidin was utilized to label a 3T3 cell culture for intracellular filamentous actin, while DAPI was used to target DNA in the cell nuclei. (olympus-lifescience.com)
Induce1
- Taken together, these results demonstrated that avicularin may inhibit the inflammatory response, prevent cell viability and induce apoptosis in human RA synovial cells through preventing the activation of the MEK/NF‑κB pathway. (spandidos-publications.com)
Genes1
- PPARγ is an important transcription factor that drives the expression of the genes that are needed to convert a precursor cell to a mature adipocyte. (elifesciences.org)
Substrate3
- Michaelis-Menten's analysis of the NIH-3T3 microsomes indicated a Km of 10.9 µM using E 1 S as substrate. (cotinis.com)
- This is consistent with the idea that cortisol inhibits STS in NIH-3T3 cells through a regulatory mechanism rather than by substrate inhibition. (cotinis.com)
- We show here that cells can respond to protease/substrate imbalance in this compartment by de novo expression of multiple lysosomal hydrolases. (nature.com)
Tissue5
- AQP1 was overexpressed in 62% (13 of 21) and 75% (6 of 8) of adenocarcinoma and bronchoalveolar carcinoma, respectively, whereas all cases of squamous cell carcinoma and normal lung tissue were negative. (nih.gov)
- Endosomes and lysosomes are now known to participate in multiple aspects of cell and tissue physiology besides their classical role in degradation of endocytosed substrates. (nature.com)
- Distinct phenotypes of cancer cells on tissue matrix gel. (sigmaaldrich.com)
- The effect on expression of tissue plasminogen activator was also analysed in the cell culture. (hindawi.com)
- In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)
Protein4
- We next examined the distribution of the AQP1 protein in several types of primary lung tumors (16 squamous cell carcinomas, 21 adenocarcinomas, and 7 bronchoalveolar carcinomas) by immunohistochemical staining. (nih.gov)
- Microtubule depolymerization upon nocodazole treatment leads to a loss of RhoG protein from the cell periphery associated with a reversal of the RhoG phenotype, whereas PDGF or bradykinin stimulation of nocodazole-treated cells could still promote Rac1- and Cdc42Hs-dependent cytoskeletal reorganization. (cnrs.fr)
- analyzed this process in mouse cells and identified a protein called TRIM23 that is produced in precursor cells. (elifesciences.org)
- In addition, these data suggested that avicularin prevented the activation of the mitogen‑activated protein kinase kinase (MEK)/nuclear factor kappa light‑chain‑enhancer of activated B‑cells (NF‑κB) pathway activated by TNF‑α. (spandidos-publications.com)
Induction1
- Two day post-confluent 3T3-L1 cells were treated for 8 days with induction agents (MDI) and exposed to TBBPA. (nih.gov)
Growth3
- A toxic chemical, regardless of site or mechanism of action, will interfere with this process and result in a reduction of the growth rate as reflected by cell number. (nih.gov)
- Cytotoxicity is expressed as a concentration dependent reduction of the uptake of the NR after chemical exposure thus providing a sensitive, integrated signal of both cell integrity and growth inhibition. (nih.gov)
- FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. (peprotech.com)
Viability1
- It was also identified that TNF‑α administration significantly promoted MH7A cell viability and inhibited cell apoptosis, and avicularin treatment dose‑dependently inhibited MH7A cell viability and induced cell apoptosis. (spandidos-publications.com)
Connective1
- Breast cancer cells invading the connective tissues outside the mammary lobule or duct immerse in a reservoir of extracellular matrix (ECM) that is structurally and biochemically distinct from that of their site of origin. (sigmaaldrich.com)
Enzymes1
- The cells were simultaneously stained with Alexa Fluor 488 conjugated to concanavalin A, a lectin displaying very high affinity for specific carbohydrate residues in glycoproteins, enzymes, and cell membranes. (olympus-lifescience.com)
Membranes1
- NR is a weak cationic dye that readily penetrates cell membranes by non-ionic diffusion and accumulates intracellularly in lysosomes. (nih.gov)
Regulatory1
- Targeted delivery of regulatory macrophages to lymph nodes interferes with T cell priming by preventing the formation of stable immune synapses. (peprotech.com)
Concentrations1
- MH7A cells were then treated with various concentrations (10, 30, 100 and 300 µM) of avicularin. (spandidos-publications.com)
Protocol1
- Protocol for automated production of human stem cell derived liver spheres. (peprotech.com)
MCF72
- In ICC/IF cytoplasmic staining can be seen in MCF7 cells. (novusbio.com)
- Confocal immunofluorescent analysis of MCF7 (positive, left) and SNB19 (negative, right) cells using RAB11FIP1 (D9D8P) Rabbit mAb (green). (cellsignal.com)
Collagen1
- However, collagen gels exhibit unstable geometrical properties, arising from extensive cell-mediated contraction. (sigmaaldrich.com)
Lines3
- All 10 CdCl(2)-induced transformed cell lines formed fibrosarcomas in nude mice within 39 days of inoculation. (cdc.gov)
- Flow cytometric analysis of Daudi cells (blue) and MJ cells (green) using Lamin A/C (4C11) Mouse mAb (solid lines) or concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (dashed lines). (cellsignal.com)
- Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). (nih.gov)
Culture4
- Prior to animal study, the enzyme safety was tested using cell culture. (hindawi.com)
- Experiment with cell culture confirmed the enzyme safety at the concentration used and increased expression of tPA. (hindawi.com)
- Safety of the bacterial enzyme from the Indonesian fermented Oncom was tested using cell culture and experimental rats, while the effect of Stenotrophomonas enzyme on degrading thrombus was observed using the experimental rats. (hindawi.com)
- The TESA SELEX was performed using infected NIH-3T3 cell culture supernatant. (nih.gov)
Mice1
- Similar lysosomal adaptations are seen in mice and cells expressing a constitutively active form of STAT3. (nature.com)
Confocal3
- Confocal immunofluorescent analysis of RAW 264.7 (left) and C2C12 (right) cells using IKKepsilon (D61F9) XP ® Rabbit mAb (green). (cellsignal.com)
- Confocal immunofluorescent analysis of 32D cells (left) and C2C12 cells (right), using HS1 (D5A9) XP ® Rabbit mAb (green). (cellsignal.com)
- Confocal immunofluorescent analysis of A549 (left) and Hep G2 (right) cells using IQGAP1 (D8K4X) XP ® Rabbit mAb (green). (cellsignal.com)
Subject1
- Survey experience is sufficient to conclude that laboratory aerosol infection does not occur in the course of routine work with cell cultures and animals not subject to chronic infection. (cdc.gov)
Reduction2
- The reduction in STS activity by dexamethasone in whole cells was rversed by the glucocorticoid receptor antagonist RU-486, indicating that glucocorticoid downregulation of STS activity is receptor mediated. (cotinis.com)
- Plaque reduction in Vero cells. (cdc.gov)
Lysosomal3
- Alterations of the cell surface or the sensitive lysosomal membrane lead to lysosomal fragility and other changes that gradually become irreversible. (nih.gov)
- Diverse cellular processes depend on the lysosomal protease system but how cells regulate lysosomal proteolytic capacity is only partly understood. (nature.com)
- Our results reveal how cells can increase lysosomal protease capacity under 'fed' rather than 'starved' conditions that activate the TFEB system. (nature.com)
High1
- We also found that microsomes prepared from NIH-3T3 cells had relatively high STS activity and that cytosols had low activity , consistent with the known distribution of this enzyme to the endoplasmic reticulum. (cotinis.com)
Found1
- Increased oxidative stress in accumulated fat was found in 3T3-L1 cells . (bvsalud.org)
Healthy1
- Obesity is caused when excess fat is stored in fat cells, and overweight individuals have larger fat cells compared to healthy weight people. (elifesciences.org)
Expression1
- Berberine inhibited increased oxidative stress in 3T3-L1 cells by suppressing reactive oxygen species (ROS) production , and increased glutathione peroxidase (GPx) gene expression and GPx activity. (bvsalud.org)
