Authorship: The profession of writing. Also the identity of the writer as the creator of a literary production.Knowledge Bases: Collections of facts, assumptions, beliefs, and heuristics that are used in combination with databases to achieve desired results, such as a diagnosis, an interpretation, or a solution to a problem (From McGraw Hill Dictionary of Scientific and Technical Terms, 6th ed).Editorial Policies: The guidelines and policy statements set forth by the editor(s) or editorial board of a publication.Scientific Misconduct: Intentional falsification of scientific data by presentation of fraudulent or incomplete or uncorroborated findings as scientific fact.Publishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.NIH 3T3 Cells: A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. The cells are useful for DNA transfection and transformation studies. (From ATCC [Internet]. Virginia: American Type Culture Collection; c2002 [cited 2002 Sept 26]. Available from http://www.atcc.org/)Game Theory: Theoretical construct used in applied mathematics to analyze certain situations in which there is an interplay between parties that may have similar, opposed, or mixed interests. In a typical game, decision-making "players," who each have their own goals, try to gain advantage over the other parties by anticipating each other's decisions; the game is finally resolved as a consequence of the players' decisions.Dominica: An island republic of the West Indies. Its capital is Roseau. It was discovered in 1493 by Columbus and held at different times by the French and the British in the 18th century. A member of the West Indies Federation, it achieved internal self-government in 1967 but became independent in 1978. It was named by Columbus who discovered it on Sunday, Domingo in Spanish, from the Latin Dominica dies, the Lord's Day. (From Webster's New Geographical Dictionary, 1988, p338 & Room, Brewer's Dictionary of Names, 1992, p151)Cooperative Behavior: The interaction of two or more persons or organizations directed toward a common goal which is mutually beneficial. An act or instance of working or acting together for a common purpose or benefit, i.e., joint action. (From Random House Dictionary Unabridged, 2d ed)Expert Systems: Computer programs based on knowledge developed from consultation with experts on a problem, and the processing and/or formalizing of this knowledge using these programs in such a manner that the problems may be solved.Hemangiosarcoma: A rare malignant neoplasm characterized by rapidly proliferating, extensively infiltrating, anaplastic cells derived from blood vessels and lining irregular blood-filled or lumpy spaces. (Stedman, 25th ed)Reticulin: A scleroprotein fibril consisting mostly of type III collagen. Reticulin fibrils are extremely thin, with a diameter of between 0.5 and 2 um. They are involved in maintaining the structural integrity in a variety of organs.Glass: Hard, amorphous, brittle, inorganic, usually transparent, polymerous silicate of basic oxides, usually potassium or sodium. It is used in the form of hard sheets, vessels, tubing, fibers, ceramics, beads, etc.Mice, Inbred BALB CCell Transformation, Neoplastic: Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.Cell Adhesion: Adherence of cells to surfaces or to other cells.Polycarboxylate Cement: Water-soluble low-molecular-weight polymers of acrylic or methacrylic acid that form solid, insoluble products when mixed with specially prepared ZnO powder. The resulting cement adheres to dental enamel and is also used as a luting agent.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Embryo, Mammalian: The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Deoxy SugarsDeoxyglucose: 2-Deoxy-D-arabino-hexose. An antimetabolite of glucose with antiviral activity.Cell Transformation, Viral: An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.Abelson murine leukemia virus: A replication-defective strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) capable of transforming lymphoid cells and producing a rapidly progressing lymphoid leukemia after superinfection with FRIEND MURINE LEUKEMIA VIRUS; MOLONEY MURINE LEUKEMIA VIRUS; or RAUSCHER VIRUS.Cell Transformation, Neoplastic: Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.Herpesvirus 4, Human: The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Biological Transport, Active: The movement of materials across cell membranes and epithelial layers against an electrochemical gradient, requiring the expenditure of metabolic energy.Monosaccharide Transport Proteins: A large group of membrane transport proteins that shuttle MONOSACCHARIDES across CELL MEMBRANES.Mevalonic AcidCell Cycle: The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.Hydroxymethylglutaryl CoA Reductases: Enzymes that catalyze the reversible reduction of alpha-carboxyl group of 3-hydroxy-3-methylglutaryl-coenzyme A to yield MEVALONIC ACID.Dolichol: Eicosamethyl octacontanonadecasen-1-o1. Polyprenol found in animal tissues that contains about 20 isoprene residues, the one carrying the alcohol group being saturated.Lovastatin: A fungal metabolite isolated from cultures of Aspergillus terreus. The compound is a potent anticholesteremic agent. It inhibits 3-hydroxy-3-methylglutaryl coenzyme A reductase (HYDROXYMETHYLGLUTARYL COA REDUCTASES), which is the rate-limiting enzyme in cholesterol biosynthesis. It also stimulates the production of low-density lipoprotein receptors in the liver.Cholesterol: The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.Hydroxycholesterols: Cholesterol which is substituted by a hydroxy group in any position.Hydroxymethylglutaryl-CoA Reductase Inhibitors: Compounds that inhibit HMG-CoA reductases. They have been shown to directly lower cholesterol synthesis.GlutaratesCell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.BooksPublishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.MEDLINE: The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).Serial Publications: Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)Biological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Chromosome Mapping: Any method used for determining the location of and relative distances between genes on a chromosome.Abstracting and Indexing as Topic: Activities performed to identify concepts and aspects of published information and research reports.Information Storage and Retrieval: Organized activities related to the storage, location, search, and retrieval of information.Insulin Receptor Substrate Proteins: A structurally-related group of signaling proteins that are phosphorylated by the INSULIN RECEPTOR PROTEIN-TYROSINE KINASE. The proteins share in common an N-terminal PHOSPHOLIPID-binding domain, a phosphotyrosine-binding domain that interacts with the phosphorylated INSULIN RECEPTOR, and a C-terminal TYROSINE-rich domain. Upon tyrosine phosphorylation insulin receptor substrate proteins interact with specific SH2 DOMAIN-containing proteins that are involved in insulin receptor signaling.Receptor, Insulin: A cell surface receptor for INSULIN. It comprises a tetramer of two alpha and two beta subunits which are derived from cleavage of a single precursor protein. The receptor contains an intrinsic TYROSINE KINASE domain that is located within the beta subunit. Activation of the receptor by INSULIN results in numerous metabolic changes including increased uptake of GLUCOSE into the liver, muscle, and ADIPOSE TISSUE.Oxidative Stress: A disturbance in the prooxidant-antioxidant balance in favor of the former, leading to potential damage. Indicators of oxidative stress include damaged DNA bases, protein oxidation products, and lipid peroxidation products (Sies, Oxidative Stress, 1991, pxv-xvi).Autophagy: The segregation and degradation of damaged or unwanted cytoplasmic constituents by autophagic vacuoles (cytolysosomes) composed of LYSOSOMES containing cellular components in the process of digestion; it plays an important role in BIOLOGICAL METAMORPHOSIS of amphibians, in the removal of bone by osteoclasts, and in the degradation of normal cell components in nutritional deficiency states.Insulin: A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).PhosphoproteinsReactive Oxygen Species: Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of signal transduction and gene expression, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Phosphatidylinositol 3-Kinases: Phosphotransferases that catalyzes the conversion of 1-phosphatidylinositol to 1-phosphatidylinositol 3-phosphate. Many members of this enzyme class are involved in RECEPTOR MEDIATED SIGNAL TRANSDUCTION and regulation of vesicular transport with the cell. Phosphatidylinositol 3-Kinases have been classified both according to their substrate specificity and their mode of action within the cell.Cytoskeleton: The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.Actins: Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.Cell Line, Transformed: Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.Telomerase: An essential ribonucleoprotein reverse transcriptase that adds telomeric DNA to the ends of eukaryotic CHROMOSOMES.Tubulin: A microtubule subunit protein found in large quantities in mammalian brain. It has also been isolated from SPERM FLAGELLUM; CILIA; and other sources. Structurally, the protein is a dimer with a molecular weight of approximately 120,000 and a sedimentation coefficient of 5.8S. It binds to COLCHICINE; VINCRISTINE; and VINBLASTINE.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Actin Cytoskeleton: Fibers composed of MICROFILAMENT PROTEINS, which are predominately ACTIN. They are the smallest of the cytoskeletal filaments.Organelles: Specific particles of membrane-bound organized living substances present in eukaryotic cells, such as the MITOCHONDRIA; the GOLGI APPARATUS; ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Cercaria: The free-swimming larval forms of parasites found in an intermediate host.Microscopy, Fluorescence: Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.Microscopy: The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.Fluorescent Dyes: Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.Theaceae: A plant family of the order THEALES, subclass Dilleniidae, class Magnoliopsida, best known for CAMELLIA SINENSIS, which is the source of Oriental TEA.Microscopy, Confocal: A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.Phalloidine: Very toxic polypeptide isolated mainly from AMANITA phalloides (Agaricaceae) or death cup; causes fatal liver, kidney and CNS damage in mushroom poisoning; used in the study of liver damage.Microscopy, Fluorescence, Multiphoton: Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore. Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged.Microscopy, Electron, Scanning: Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.Image Processing, Computer-Assisted: A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.
(1/10733) Transformation mediated by RhoA requires activity of ROCK kinases.

BACKGROUND: The Ras-related GTPase RhoA controls signalling processes required for cytoskeletal reorganisation, transcriptional regulation, and transformation. The ability of RhoA mutants to transform cells correlates not with transcription but with their ability to bind ROCK-I, an effector kinase involved in cytoskeletal reorganisation. We used a recently developed specific ROCK inhibitor, Y-27632, and ROCK truncation mutants to investigate the role of ROCK kinases in transcriptional activation and transformation. RESULTS: In NIH3T3 cells, Y-27632 did not prevent the activation of serum response factor, transcription of c-fos or cell cycle re-entry following serum stimulation. Repeated treatment of NIH3T3 cells with Y-27632, however, substantially disrupted their actin fibre network but did not affect their growth rate. Y-27632 blocked focus formation by RhoA and its guanine-nucleotide exchange factors Dbl and mNET1. It did not affect the growth rate of cells transformed by Dbl and mNET1, but restored normal growth control at confluence and prevented their growth in soft agar. Y-27632 also significantly inhibited focus formation by Ras, but had no effect on the establishment or maintenance of transformation by Src. Furthermore, it significantly inhibited anchorage-independent growth of two out of four colorectal tumour cell lines. Consistent with these data, a truncated ROCK derivative exhibited weak ability to cooperate with activated Raf in focus formation assays. CONCLUSIONS: ROCK signalling is required for both the establishment and maintenance of transformation by constitutive activation of RhoA, and contributes to the Ras-transformed phenotype. These observations provide a potential explanation for the requirement for Rho in Ras-mediated transformation. Moreover, the inhibition of ROCK kinases may be of therapeutic use.  (+info)

(2/10733) Polarized distribution of Bcr-Abl in migrating myeloid cells and co-localization of Bcr-Abl and its target proteins.

Bcr-Abl plays a critical role in the pathogenesis of Philadelphia chromosome-positive leukemia. Although a large number of substrates and interacting proteins of Bcr-Abl have been identified, it remains unclear whether Bcr-Abl assembles multi-protein complexes and if it does where these complexes are within cells. We have investigated the localization of Bcr-Abl in 32D myeloid cells attached to the extracellular matrix. We have found that Bcr-Abl displays a polarized distribution, colocalizing with a subset of filamentous actin at trailing portions of migrating 32D cells, and localizes on the cortical F-actin and on vesicle-like structures in resting 32D cells. Deletion of the actin binding domain of Bcr-Abl (Bcr-AbI-AD) dramatically enhances the localization of Bcr-Abl on the vesicle-like structures. These distinct localization patterns of Bcr-Abl and Bcr-Abl-AD enabled us to examine the localization of Bcr-Abl substrate and interacting proteins in relation to Bcr-Abl. We found that a subset of biochemically defined target proteins of Bcr-Abl redistributed and co-localized with Bcr-Abl on F-actin and on vesicle-like structures. The co-localization of signaling proteins with Bcr-Abl at its sites of localization supports the idea that Bcr-Abl forms a multi-protein signaling complex, while the polarized distribution and vesicle-like localization of Bcr-Abl may play a role in leukemogenesis.  (+info)

(3/10733) Expression of the naturally occurring truncated trkB neurotrophin receptor induces outgrowth of filopodia and processes in neuroblastoma cells.

We have investigated the effects of the truncated trkB receptor isoform T1 (trkB.T1) by transient transfection into mouse N2a neuroblastoma cells. We observed that expression of trkB.T1 leads to a striking change in cell morphology characterized by outgrowth of filopodia and processes. A similar morphological response was also observed in SH-SY5Y human neuroblastoma cells and NIH3T3 fibroblasts transfected with trkB.T1. N2a cells lack endogenous expression of trkB isoforms, but express barely detectable amounts of its ligands, brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT-4). The morphological change was ligand-independent, since addition of exogenous BDNF or NT-4 or blockade of endogenous trkB ligands did not influence this response. Filopodia and process outgrowth was significantly suppressed when full-length trkB.TK+ was cotransfected together with trkB.T1 and this inhibitory effect was blocked by tyrosine kinase inhibitor K252a. Transfection of trkB.T1 deletion mutants showed that the morphological response is dependent on the extracellular, but not the intracellular domain of the receptor. Our results suggest a novel ligand-independent role for truncated trkB in the regulation of cellular morphology.  (+info)

(4/10733) Plasma membrane recruitment of RalGDS is critical for Ras-dependent Ral activation.

In COS cells, Ral GDP dissociation stimulator (RalGDS)-induced Ral activation was stimulated by RasG12V or a Rap1/Ras chimera in which the N-terminal region of Rap1 was ligated to the C-terminal region of Ras but not by Rap1G12V or a Ras/Rap1 chimera in which the N-terminal region of Ras was ligated to the C-terminal region of Rap1, although RalGDS interacted with these small GTP-binding proteins. When RasG12V, Ral and the Rap1/Ras chimera were individually expressed in NIH3T3 cells, they localized to the plasma membrane. Rap1Q63E and the Ras/Rap1 chimera were detected in the perinuclear region. When RalGDS was expressed alone, it was abundant in the cytoplasm. When coexpressed with RasG12V or the Rap1/Ras chimera, RalGDS was detected at the plasma membrane, whereas when coexpressed with Rap1Q63E or the Ras/Rap1 chimera, RalGDS was observed in the perinuclear region. RalGDS which was targeted to the plasma membrane by the addition of Ras farnesylation site (RalGDS-CAAX) activated Ral in the absence of RasG12V. Although RalGDS did not stimulate the dissociation of GDP from Ral in the absence of the GTP-bound form of Ras in a reconstitution assay using the liposomes, RalGDS-CAAX could stimulate it without Ras. RasG12V activated Raf-1 when they were coexpressed in Sf9 cells, whereas RasG12V did not affect the RalGDS activity. These results indicate that Ras recruits RalGDS to the plasma membrane and that the translocated RalGDS induces the activation of Ral, but that Rap1 does not activate Ral due to distinct subcellular localization.  (+info)

(5/10733) Id helix-loop-helix proteins inhibit nucleoprotein complex formation by the TCF ETS-domain transcription factors.

The Id subfamily of helix-loop-helix (HLH) proteins plays a fundamental role in the regulation of cellular proliferation and differentiation. Id proteins are thought to inhibit differentiation mainly through interaction with other HLH proteins and by blocking their DNA-binding activity. Members of the ternary complex factor (TCF) subfamily of ETS-domain proteins have key functions in regulating immediate-early gene expression in response to mitogenic stimulation. TCFs form DNA-bound complexes with the serum response factor (SRF) and are direct targets of MAP kinase (MAPK) signal transduction cascades. In this study we demonstrate functional interactions between Id proteins and TCFs. Ids bind to the ETS DNA-binding domain and disrupt the formation of DNA-bound complexes between TCFs and SRF on the c-fos serum response element (SRE). Inhibition occurs by disrupting protein-DNA interactions with the TCF component of this complex. In vivo, the Id proteins cause down-regulation of the transcriptional activity mediated by the TCFs and thereby block MAPK signalling to SREs. Therefore, our results demonstrate a novel facet of Id function in the coordination of mitogenic signalling and cell cycle entry.  (+info)

(6/10733) A cytomegalovirus glycoprotein re-routes MHC class I complexes to lysosomes for degradation.

Mouse cytomegalovirus (MCMV) early gene expression interferes with the major histocompatibility complex class I (MHC class I) pathway of antigen presentation. Here we identify a 48 kDa type I transmembrane glycoprotein encoded by the MCMV early gene m06, which tightly binds to properly folded beta2-microglobulin (beta2m)-associated MHC class I molecules in the endoplasmic reticulum (ER). This association is mediated by the lumenal/transmembrane part of the protein. gp48-MHC class I complexes are transported out of the ER, pass the Golgi, but instead of being expressed on the cell surface, they are redirected to the endocytic route and rapidly degraded in a Lamp-1(+) compartment. As a result, m06-expressing cells are impaired in presenting antigenic peptides to CD8(+) T cells. The cytoplasmic tail of gp48 contains two di-leucine motifs. Mutation of the membrane-proximal di-leucine motif of gp48 restored surface expression of MHC class I, while mutation of the distal one had no effect. The results establish a novel viral mechanism for downregulation of MHC class I molecules by directly binding surface-destined MHC complexes and exploiting the cellular di-leucine sorting machinery for lysosomal degradation.  (+info)

(7/10733) Activation of c-Jun N-terminal kinase 1 by UV irradiation is inhibited by wortmannin without affecting c-iun expression.

Activation of c-Jun N-terminal kinases (JNKs)/stress-activated protein kinases is an early response of cells upon exposure to DNA-damaging agents. JNK-mediated phosphorylation of c-Jun is currently understood to stimulate the transactivating potency of AP-1 (e.g., c-Jun/c-Fos; c-Jun/ATF-2), thereby increasing the expression of AP-1 target genes. Here we show that stimulation of JNK1 activity is not a general early response of cells exposed to genotoxic agents. Treatment of NIH 3T3 cells with UV light (UV-C) as well as with methyl methanesulfonate (MMS) caused activation of JNK1 and an increase in c-Jun protein and AP-1 binding activity, whereas antineoplastic drugs such as mafosfamide, mitomycin C, N-hydroxyethyl-N-chloroethylnitrosourea, and treosulfan did not elicit this response. The phosphatidylinositol 3-kinase inhibitor wortmannin specifically blocked the UV-stimulated activation of JNK1 but did not affect UV-driven activation of extracellular regulated kinase 2 (ERK2). To investigate the significance of JNK1 for transactivation of c-jun, we analyzed the effect of UV irradiation on c-jun expression under conditions of wortmannin-mediated inhibition of UV-induced stimulation of JNK1. Neither the UV-induced increase in c-jun mRNA, c-Jun protein, and AP-1 binding nor the activation of the collagenase and c-jun promoters was affected by wortmannin. In contrast, the mitogen-activated protein kinase/ERK kinase inhibitor PD98056, which blocked ERK2 but not JNK1 activation by UV irradiation, impaired UV-driven c-Jun protein induction and AP-1 binding. Based on the data, we suggest that JNK1 stimulation is not essential for transactivation of c-jun after UV exposure, whereas activation of ERK2 is required for UV-induced signaling leading to elevated c-jun expression.  (+info)

(8/10733) Cell growth inhibition by farnesyltransferase inhibitors is mediated by gain of geranylgeranylated RhoB.

Recent results have shown that the ability of farnesyltransferase inhibitors (FTIs) to inhibit malignant cell transformation and Ras prenylation can be separated. We proposed previously that farnesylated Rho proteins are important targets for alternation by FTIs, based on studies of RhoB (the FTI-Rho hypothesis). Cells treated with FTIs exhibit a loss of farnesylated RhoB but a gain of geranylgeranylated RhoB (RhoB-GG), which is associated with loss of growth-promoting activity. In this study, we tested whether the gain of RhoB-GG elicited by FTI treatment was sufficient to mediate FTI-induced cell growth inhibition. In support of this hypothesis, when expressed in Ras-transformed cells RhoB-GG induced phenotypic reversion, cell growth inhibition, and activation of the cell cycle kinase inhibitor p21WAF1. RhoB-GG did not affect the phenotype or growth of normal cells. These effects were similar to FTI treatment insofar as they were all induced in transformed cells but not in normal cells. RhoB-GG did not promote anoikis of Ras-transformed cells, implying that this response to FTIs involves loss-of-function effects. Our findings corroborate the FTI-Rho hypothesis and demonstrate that gain-of-function effects on Rho are part of the drug mechanism. Gain of RhoB-GG may explain how FTIs inhibit the growth of human tumor cells that lack Ras mutations.  (+info)

*  3T3 cells
The 3T3 cell line has become the standard fibroblast cell line. Todaro and Green originally obtained their 3T3 cells from Swiss ... Cellosaurus entry for 3T3 Nikon MicroscopyU Digital Video Gallery: 3T3 Cell Motility - a set of films of 3T3 cells in culture. ... These cells are also contact inhibited. The cells are sensitive to sarcoma virus and leukemia virus focus formation. 3T3 cells ... 3T3' cells. Specifically, "3T3-L1" is one of the current lines. Swiss 3T3 can be inhibited by temazepam and other ...
*  Adipogenesis
Green, Howard; Kehinde, Olaniyi (28 February 1974). "Sublines of mouse 3T3 cells that accumulate lipid". Cell. 1 (3): 113-116. ... "Spontaneous heritable changes leading to increased adipose conversion in 3T3 cells". Cell. 7 (1): 105-13. doi:10.1016/0092-8674 ... 3T3-F4424 cells when implanted into an athymic (nude) mice gave rise to fat pads that were similar to endogenous white adipose ... Spiegelman, BM; Green, H (Sep 25, 1980). "Control of specific protein biosynthesis during the adipose conversion of 3T3 cells ...
*  Fibroblast
3T3 cells Fibrocartilage callus Fibrous connective tissue "Fibroblast". Genetics Home Reference. U.S. National Library of ... The suffix "-blast" is used in cellular biology to denote a stem cell or a cell in an activated state of metabolism. ... Receptors on the surface of fibroblasts also allow regulation of hematopoietic cells and provide a pathway for immune cells to ... Mouse embryonic fibroblasts (MEFs) are often used as "feeder cells" in human embryonic stem cell research. However, many ...
*  PEPD
Expression of the mutant enzyme in NIH 3T3 cells". The Journal of Clinical Investigation. 86 (1): 351-5. doi:10.1172/JCI114708 ... Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. PMID ... Cell. 154 (2): 452-64. doi:10.1016/j.cell.2013.06.022. PMC 3717207 . PMID 23870131. "Infection and Immunity Immunophenotyping ( ... Surazyński A, Pałka J (2002). "FAK-independent regulation of prolidase activity and collagen biosynthesis in MCF-7 cells". ...
*  ERBB4
Cohen BD, Green JM, Foy L, Fell HP (Mar 1996). "HER4-mediated biological and biochemical properties in NIH 3T3 cells. Evidence ... Cell. 127 (1): 185-97. doi:10.1016/j.cell.2006.07.037. PMID 17018285. "Entrez Gene: ERBB4 v-erb-a erythroblastic leukemia viral ... Culouscou JM, Plowman GD, Carlton GW, Green JM, Shoyab M (Sep 1993). "Characterization of a breast cancer cell differentiation ... Carpenter G (Mar 2003). "ErbB-4: mechanism of action and biology". Experimental Cell Research. 284 (1): 66-77. doi:10.1016/ ...
*  MAN2A1
Moremen KW, Touster O (Jun 1985). "Biosynthesis and modification of Golgi mannosidase II in HeLa and 3T3 cells". The Journal of ... Velasco A, Hendricks L, Moremen KW, Tulsiani DR, Touster O, Farquhar MG (Jul 1993). "Cell type-dependent variations in the ... The Journal of Cell Biology. 115 (6): 1521-34. doi:10.1083/jcb.115.6.1521. PMC 2289207 . PMID 1757461. Oka T, Ungar D, Hughson ... Molecular Biology of the Cell. 15 (5): 2423-35. doi:10.1091/mbc.E03-09-0699. PMC 404034 . PMID 15004235. "Entrez Gene: MAN2A1 ...
*  Rho family of GTPases
Virtually all eukaryotic cells form such processes upon Rho activation. Fibroblasts such as Swiss 3T3 cells are often used to ... Before the cell can bud, Cdc42 is used to locate the region of the cell's membrane that will begin to bulge into the new cell. ... Before cells can undergo key processes such as budding, mitosis, or locomotion, it must have some manner of cell polarity. One ... Animal cells form many different shapes based on their function and location in the body. Rho proteins help cells regulate ...
*  C-Raf
Kolch W, Heidecker G, Lloyd P, Rapp UR (January 1991). "Raf-1 protein kinase is required for growth of induced NIH/3T3 cells". ... Romero F, Martínez-A C, Camonis J, Rebollo A (June 1999). "Aiolos transcription factor controls cell death in T cells by ... cell migration, inhibition of apoptosis, and cell differentiation. Hereditary gain-of-function mutations of c-Raf are ... "X-linked and cellular IAPs modulate the stability of C-RAF kinase and cell motility". Nat. Cell Biol. 10 (12): 1447-55. doi: ...
*  Cytosol
"Hindered diffusion of inert tracer particles in the cytoplasm of mouse 3T3 cells". Proc. Natl. Acad. Sci. U.S.A. 84 (14): 4910- ... Examples of these processes include signal transduction from the cell membrane to sites within the cell, such as the cell ... cell signaling, and the generation of action potentials in excitable cells such as endocrine, nerve and muscle cells. The ... without damaging the other cell membranes, only about one quarter of cell protein was released. These cells were also able to ...
*  Fibroblast growth factor
Armelin HA (Sep 1973). "Pituitary extracts and steroid hormones in the control of 3T3 cell growth". Proceedings of the National ... For example: FGF15 and FGF19 (FGF15/19) are produced by intestinal cells but act on FGFR4-expressing liver cells to ... "Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth". Nature. 249 (453 ... FGF23 is produced by bone but acts on FGFR1-expressing kidney cells to regulate the synthesis of vitamin D and phosphate ...
*  Actinin alpha 1
"Modulation of alpha-actinin levels affects cell motility and confers tumorigenicity on 3T3 cells". J. Cell Sci. 107 (7): 1773- ... "Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin". J. Cell Biol. 130 (1): 67 ... Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal ... and alpha-actinin-binding protein localized at cell-cell adherens junctions". J. Biol. Chem. 278 (6): 4103-11. doi:10.1074/jbc. ...
*  Cyclin D1
"Requirement for ras proto-oncogene function during serum-stimulated growth of NIH 3T3 cells". Nature. 313 (5999): 241-3. ... Cyclin D1 is expressed in all adult human tissues with the exception of cells derived from bone marrow stem cell lines (both ... Amplification of the CCND1 gene is present in: non-small cell lung cancers (30-46%) head and neck squamous cell carcinomas (30- ... Chromosomal translocation around the cyclin D1 gene locus is often seen in B mantle cell lymphoma. In mantle cell lymphoma, ...
*  MDM4
"Entrez Gene: MDM4 Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse)". Strachan GD, Jordan-Sciutto KL, ... regulates p53-induced growth arrest and neuronal cell death during early embryonic mouse development". Mol. Cell. Biol. 22 (15 ... Cell. Biol. 22 (21): 7562-71. doi:10.1128/MCB.22.21.7562-7571.2002. PMC 135668 . PMID 12370303. Badciong JC, Haas AL (2003). " ... Sabbatini P, McCormick F (2002). "MDMX inhibits the p300/CBP-mediated acetylation of p53". DNA Cell Biol. 21 (7): 519-25. doi: ...
*  Bone morphogenetic protein 4
"Bone morphogenetic protein-2 causes commitment and differentiation in C3H10T1/2 and 3T3 cells". Growth Factors. 9 (1): 57-71. ... "Regeneration of Thyroid Function by Transplantation of Differentiated Pluripotent Stem Cells". Cell Stem Cell. 17 (5): 527-42. ... If these cells also receive signals from FGF, they will differentiate into the spinal cord; in the absence of FGF the cells ... Also in conjunction with FGF2 it can produce progenitor thyroid cells from pluripotent stem cells in mice and hmans. BMP4 has ...
*  Ravi Iyengar
Ram, P. T.; Horvath, C. M.; Iyengar, R (2000). "Stat3-mediated transformation of NIH-3T3 cells by the constitutively active ... His laboratory focuses on how cell signals are routed and processed through cellular signaling networks within cells to ... "Stat3-mediated transformation of NIH-3T3 cells by the constitutively active Q205L Galphao protein". Science. 287 (5450): 142-4 ... Cell. 133 (4): 666-80. doi:10.1016/j.cell.2008.04.025. PMC 2728678 . PMID 18485874. Bhalla, U. S.; Iyengar, R (1999). "Emergent ...
*  ABI1
"Human spectrin Src homology 3 domain binding protein 1 regulates macropinocytosis in NIH 3T3 cells". J. Cell Sci. 113 (21): ... Cell. Neurosci. 16 (3): 244-57. doi:10.1006/mcne.2000.0865. PMID 10995551. Fan PD, Goff SP (2000). "Abl Interactor 1 Binds to ... Cell. Biol. 20 (20): 7591-601. doi:10.1128/MCB.20.20.7591-7601.2000. PMC 86315 . PMID 11003655. Xu J, Ziemnicka D, Merz GS, ... Cell. 15 (1): 91-8. doi:10.1091/mbc.E03-06-0427. PMC 307530 . PMID 14565974. Lanzetti L, Rybin V, Malabarba MG, Christoforidis ...
*  Muscarinic acetylcholine receptor M1
"Signaling through transforming G protein-coupled receptors in NIH 3T3 cells involves c-Raf activation. Evidence for a protein ... Cell. Biol. 14 (4): 2343-51. doi:10.1128/mcb.14.4.2343. PMC 358601 . PMID 8139539. Offermanns S, Wieland T, Homann D, Sandmann ... Burford NT, Nahorski SR (1996). "Muscarinic m1 receptor-stimulated adenylate cyclase activity in Chinese hamster ovary cells is ... Ashkenazi A, Ramachandran J, Capon DJ (1989). "Acetylcholine analogue stimulates DNA synthesis in brain-derived cells via ...
*  Phosphatidylinositol-3-phosphatase
"The discovery of a 3-phosphomonoesterase that hydrolyzes phosphatidylinositol 3-phosphate in NIH 3T3 cells". J. Biol. Chem. 264 ...
*  Muscarinic acetylcholine receptor M3
"Signaling through transforming G protein-coupled receptors in NIH 3T3 cells involves c-Raf activation. Evidence for a protein ... cell M3 muscarinic acetylcholine receptors in regulating insulin release and blood glucose homeostasis in vivo". Cell ... These receptors are highly expressed on pancreatic beta cells and are critical regulators of glucose homoestasis by modulating ... However, with respect to vasculature, activation of M3 on vascular endothelial cells causes increased synthesis of nitric oxide ...
*  Translationally-controlled tumor protein
"Transcriptional and translational control of cytoplasmic proteins after serum stimulation of quiescent Swiss 3T3 cells". ... Telerman A, Amson R. "TCTP/tpt1 - Remodeling Signaling from Stem Cell to Disease". Results and Problems in Cell Differentiation ... Expressing TCTP in U2OS (Human Bone Osteosarcoma Epithelial Cells) protected them from cell death induced by etoposide over ... Reducing TCTP (dTCTP) levels in Drosophila reduces cell size, cell number and organ size, which mimicsDrosophila Rheb (dRheb) ...
*  Hepatocyte growth factor
Kochhar KS, Johnson ME, Volpert O, Iyer AP (1995). "Evidence for autocrine basis of transformation in NIH-3T3 cells transfected ... It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, but also acts ... As well as the well-characterised effects of HGF on epithelial cells, endothelial cells and haemopoietic progenitor cells, HGF ... Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial ...
*  PPP1R12A
Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to ... Cell Biol. 3 (4): 346-52. doi:10.1038/35070019. PMID 11283607. Murányi A, Zhang R, Liu F, et al. (2001). "Myotonic dystrophy ... PPP1R12A Info with links in the Cell Migration Gateway. ... muscle and interaction of actin and myosin in nonmuscle cells. ...
*  Start point (yeast)
in 1985 provided evidence for a commitment point in Swiss 3T3 cells, or mouse embryo fibroblasts, when grown in serum-rich or ... Thus, larger cells spend less time in the Start checkpoint compared to smaller cells. Morgan, David. The Cell Cycle: Principles ... "Distinct Interactions Select and Maintain a Specific Cell Fate." Molecular Cell 43.4 (2011): 528-39. Mitosis Cell cycle S-phase ... "Kinetic Analysis of Regulatory Events in G1 Leading to Proliferation or Quiescence of Swiss 3T3 Cells." PNAS 82 (1985): 5365- ...
*  Muscarinic acetylcholine receptor M5
1994). "Signaling through transforming G protein-coupled receptors in NIH 3T3 cells involves c-Raf activation. Evidence for a ... Cell. Biochem. 228 (1-2): 57-72. doi:10.1023/A:1013368509855. PMID 11855742. Strausberg RL, Feingold EA, Grouse LH, et al. ( ... 1996). "Identification and molecular characterization of a m5 muscarinic receptor in A2058 human melanoma cells. Coupling to ... receptor subtypes and neuronal nicotinic acetylcholine receptor subunits in human mononuclear leukocytes and leukemic cell ...
*  Warren Jackson Pledger
Platelet-derived growth factor-induced alterations in vinculin and actin distribution in BALB/c-3T3 cells. J Cell Biol. 1985 ... An ordered sequence of events is required before BALB/c-3T3 cells become committed to DNA synthesis. Proc Natl Acad Sci U S A. ... Induction of DNA synthesis in BALB/c 3T3 cells by serum components: reevaluation of the commitment process. Proc Natl Acad Sci ... A model of cell cycle control: sequential events regulated by growth factors. Mol Cell Endocrinol. 1983 Aug;31(2-3):167-86. ...
*  NOL1
Overexpression of p120 leads to malignant transformation of 3T3 cells while treatment with antisense p120 mRNA causes the ... "Increased growth of NIH/3T3 cells by transfection with human p120 complementary DNA and inhibition by a p120 antisense ... Cell Biol. 4 (7): 529-33. doi:10.1038/ncb814. PMID 12080348. Holsinger LJ, Ward K, Duffield B, Zachwieja J, Jallal B (2002). " ... Cell. 13 (11): 4100-9. doi:10.1091/mbc.E02-05-0271. PMC 133617 . PMID 12429849. Beausoleil SA, Jedrychowski M, Schwartz D, ...
3T3 cells (RSV-transformed) from Exalpha Biologicals, Inc.  3T3 cells (RSV-transformed) from Exalpha Biologicals, Inc.
3T3 cells (RSV-transformed) Application: Western Blotting Control,biological,biology supply,biology supplies,biology product ... 3T3 cells (RSV-transformed) from Exalpha Biologicals, Inc., ... 3T3 cells (RSV-transformed). Source: Cell Lysate. Form: Ready ... pLivSelect PCR Cloning Kit L (Without competent cells) from Bio S&T. 2. CopyRight pSMART VC BamHI Cloning Kit (without cells) ... CopyRight pEZ BAC Blunt Cloning Kit (without cells) from Lucigen. 10. CopyRight pEZ BAC BamHI Cloning Kit (without cells) from ...
more infohttp://bio-medicine.org/biology-products/3T3-cells--28RSV-transformed-29-from-Exalpha-Biologicals--Inc--4905-1/
WikiGenes - NIH 3T3 Cells  WikiGenes - NIH 3T3 Cells
EWS/FLI1-induced manic fringe renders NIH 3T3 cells tumorigenic [1].. *NIH 3T3 cells transfected with the cDNA of this protein ... Biological context of NIH 3T3 Cells. *Transformants of NIH 3T3 cells induced by transfection with MMTV LTR-p21 hybrid DNA have ... Disease relevance of NIH 3T3 Cells. *Overexpression of MFNG in NIH 3T3 cells renders them tumorigenic in mice with severe ... Stimulation of alpha 1 (I) procollagen gene expression in NIH-3T3 cells by the human T cell leukemia virus type 1 (HTLV-1) Tax ...
more infohttps://www.wikigenes.org/e/mesh/e/22574.html
3t3 cells Protocols and Video...  '3t3 cells' Protocols and Video...
3t3 cells' include 'Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence ... Generation of Human CD40-activated B cells', 'A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP- ... Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays (MEA)', 'A Quantitative Evaluation of Cell ... Cell Analyzer as a Tool to Delineate Molecular Pathways Involved in Neurotoxicity and Neuroprotection in a Neuronal Cell Line ...
more infohttps://www.jove.com/keyword/3t3+cells
Tumorigenic 3T3 cells maintain an alkaline intracellular pH under physiological conditions. | PNAS  Tumorigenic 3T3 cells maintain an alkaline intracellular pH under physiological conditions. | PNAS
Tumorigenic 3T3 cells maintain an alkaline intracellular pH under physiological conditions.. R J Gillies, R Martinez-Zaguilan, ... Tumorigenic 3T3 cells maintain an alkaline intracellular pH under physiological conditions.. R J Gillies, R Martinez-Zaguilan, ... Tumorigenic 3T3 cells maintain an alkaline intracellular pH under physiological conditions.. R J Gillies, R Martinez-Zaguilan, ... Tumorigenic 3T3 cells maintain an alkaline intracellular pH under physiological conditions. Message Subject (Your Name) has ...
more infohttp://www.pnas.org/content/87/19/7414
3T3 cells - Wikipedia  3T3 cells - Wikipedia
The 3T3 cell line has become the standard fibroblast cell line. Todaro and Green originally obtained their 3T3 cells from Swiss ... Cellosaurus entry for 3T3 Nikon MicroscopyU Digital Video Gallery: 3T3 Cell Motility - a set of films of 3T3 cells in culture. ... These cells are also contact inhibited. The cells are sensitive to sarcoma virus and leukemia virus focus formation. 3T3 cells ... 3T3' cells. Specifically, "3T3-L1" is one of the current lines. Swiss 3T3 can be inhibited by temazepam and other ...
more infohttps://en.wikipedia.org/wiki/3T3_cells
Vasoformative Sarcomas Arising from BALB/3T3 Cells Attached to Solid Substrates | Cancer Research  Vasoformative Sarcomas Arising from BALB/3T3 Cells Attached to Solid Substrates | Cancer Research
That the tumors were derived from BALB/3T3 cells and not host cells was proved when tumors arising in BALB/c × C57BL/6 F1 ... Vasoformative Sarcomas Arising from BALB/3T3 Cells Attached to Solid Substrates. Charles W. Boone, Noritoshi Takeichi, Meera ... Vasoformative Sarcomas Arising from BALB/3T3 Cells Attached to Solid Substrates. Charles W. Boone, Noritoshi Takeichi, Meera ... Vasoformative Sarcomas Arising from BALB/3T3 Cells Attached to Solid Substrates. Charles W. Boone, Noritoshi Takeichi, Meera ...
more infohttp://cancerres.aacrjournals.org/content/36/5/1626.long
Molecular analysis and chromosomal mapping of amplified genes isolated from a transformed mouse 3T3 cell line | SpringerLink  Molecular analysis and chromosomal mapping of amplified genes isolated from a transformed mouse 3T3 cell line | SpringerLink
... in a spontaneously transformed derivative of mouse 3T3 cells. Toward that goal, we have... ... Somatic Cell cDNA Library cDNA Clone Mapping Study Cell Hybrid These keywords were added by machine and not by the authors. ... Molecular analysis and chromosomal mapping of amplified genes isolated from a transformed mouse 3T3 cell line. ... these cells and have isolated cDNA clones representing sequences that are amplified and overexpressed in these 3T3-DM cells. ...
more infohttps://link.springer.com/article/10.1007%2FBF01535205
Regulation of the hydrolysis of phosphatidylcholine in Swiss 3T3 cells | Biochemical Society Transactions  Regulation of the hydrolysis of phosphatidylcholine in Swiss 3T3 cells | Biochemical Society Transactions
Regulation of the hydrolysis of phosphatidylcholine in Swiss 3T3 cells. Michael J. O. Wakelam, Simon J. Cook, Susan Currie, ... Regulation of the hydrolysis of phosphatidylcholine in Swiss 3T3 cells. Michael J. O. Wakelam, Simon J. Cook, Susan Currie, ... Regulation of the hydrolysis of phosphatidylcholine in Swiss 3T3 cells Message Subject (Your Name) has forwarded a page to you ...
more infohttp://www.biochemsoctrans.org/content/19/2/321
IJERPH | Free Full-Text | Nickel-Refining Fumes Induced DNA Damage and Apoptosis of NIH/3T3 Cells via Oxidative Stress  IJERPH | Free Full-Text | Nickel-Refining Fumes Induced DNA Damage and Apoptosis of NIH/3T3 Cells via Oxidative Stress
The exposure of NIH/3T3 cells to nickel-refining fumes significantly reduced cell viability and induced cell apoptotic death in ... In our research, NIH/3T3 cells were exposed to nickel-refining fumes at the concentrations of 0, 6.25, 12.50, 25, 50 and 100 μg ... DNA damage and apoptosis of NIH/3T3 cells. ... for 24 h. Cell viability, cell apoptosis, reactive oxygen ... The exposure of NIH/3T3 cells to nickel-refining fumes significantly reduced cell viability and induced cell apoptotic death in ...
more infohttp://www.mdpi.com/1660-4601/13/7/629
SV40 Virus Transformation of Mouse 3T3 Cells Does Not Specifically Enhance Sugar Transport | Science  SV40 Virus Transformation of Mouse 3T3 Cells Does Not Specifically Enhance Sugar Transport | Science
The apparent enhancement of 2-deoxy-D-glucose uptake by mouse 3T3 cells accompanying transformation by SV40 virus is not due ... SV40 Virus Transformation of Mouse 3T3 Cells Does Not Specifically Enhance Sugar Transport ... SV40 Virus Transformation of Mouse 3T3 Cells Does Not Specifically Enhance Sugar Transport ... SV40 Virus Transformation of Mouse 3T3 Cells Does Not Specifically Enhance Sugar Transport ...
more infohttp://science.sciencemag.org/content/179/4079/1238
High-resolution kinetics of transferrin acidification in BALB/c 3T3 cells: exposure to pH 6 followed by temperature-sensitive...  High-resolution kinetics of transferrin acidification in BALB/c 3T3 cells: exposure to pH 6 followed by temperature-sensitive...
The kinetics of acidification of diferric human transferrin in BALB/c mouse 3T3 cells were determined by flow cytometry using a ... High-resolution kinetics of transferrin acidification in BALB/c 3T3 cells: exposure to pH 6 followed by temperature-sensitive ... High-resolution kinetics of transferrin acidification in BALB/c 3T3 cells: exposure to pH 6 followed by temperature-sensitive ... High-resolution kinetics of transferrin acidification in BALB/c 3T3 cells: exposure to pH 6 followed by temperature-sensitive ...
more infohttps://www.pnas.org/content/84/20/7119?ijkey=7f4bb89957d2b60a0b7129994a094e8a86308160&keytype2=tf_ipsecsha
Mevalonic Acid Products as Mediators of Cell Proliferation in Simian Virus 40-transformed 3T3 Cells | Cancer Research  Mevalonic Acid Products as Mediators of Cell Proliferation in Simian Virus 40-transformed 3T3 Cells | Cancer Research
... on the cell cycle of simian virus 40-transformed 3T3 fibroblasts, designated SV-3T3 cells, were studied and compared with ... Mevalonic Acid Products as Mediators of Cell Proliferation in Simian Virus 40-transformed 3T3 Cells. Olle Larsson and Britt- ... Mevalonic Acid Products as Mediators of Cell Proliferation in Simian Virus 40-transformed 3T3 Cells ... Mevalonic Acid Products as Mediators of Cell Proliferation in Simian Virus 40-transformed 3T3 Cells ...
more infohttp://cancerres.aacrjournals.org/content/47/18/4825
The ras oncogenes increase the intrinsic resistance of NIH 3T3 cells to ionizing radiation | Science  The ras oncogenes increase the intrinsic resistance of NIH 3T3 cells to ionizing radiation | Science
The intrinsic radiation resistance (D0) of NIH 3T3 cells was markedly and significantly increased by transformation with ras ... The ras oncogenes increase the intrinsic resistance of NIH 3T3 cells to ionizing radiation ... The ras oncogenes increase the intrinsic resistance of NIH 3T3 cells to ionizing radiation ... The ras oncogenes increase the intrinsic resistance of NIH 3T3 cells to ionizing radiation ...
more infohttps://science.sciencemag.org/content/239/4840/645?ijkey=e99c1697efeec7f82977f897cd2c4cb6d5b7fe59&keytype2=tf_ipsecsha
Molecular analysis and chromosomal mapping of amplified genes isolated from a transformed mouse 3T3 cell line.  - PubMed - NCBI  Molecular analysis and chromosomal mapping of amplified genes isolated from a transformed mouse 3T3 cell line. - PubMed - NCBI
Somat Cell Mol Genet. 1987 May;13(3):235-44. Comparative Study; Research Support, U.S. Gov't, P.H.S. ... Molecular analysis and chromosomal mapping of amplified genes isolated from a transformed mouse 3T3 cell line.. Cahilly-Snyder ... these cells and have isolated cDNA clones representing sequences that are amplified and overexpressed in these 3T3-DM cells. ... in a spontaneously transformed derivative of mouse 3T3 cells. Toward that goal, we have constructed a cDNA library using RNA ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/3474784?dopt=Abstract
The effect of phosphatidic acid on the proliferation of Swiss 3T3 cells | Biochemical Society Transactions  The effect of phosphatidic acid on the proliferation of Swiss 3T3 cells | Biochemical Society Transactions
The effect of phosphatidic acid on the proliferation of Swiss 3T3 cells. C. AYLISH WOOD, LAUREN PADMORE, GEORGE K. RADDA ... The effect of phosphatidic acid on the proliferation of Swiss 3T3 cells ... The effect of phosphatidic acid on the proliferation of Swiss 3T3 cells ... The effect of phosphatidic acid on the proliferation of Swiss 3T3 cells ...
more infohttp://www.biochemsoctrans.org/content/21/4/369S
Insulin receptor substrate-1 prevents autophagy-dependent cell death caused by oxidative stress in mouse NIH/3T3 cells.  Insulin receptor substrate-1 prevents autophagy-dependent cell death caused by oxidative stress in mouse NIH/3T3 cells.
To establish cell lines with stable expression of GFP-LC3, control NIH/3T3 cells and NIH/3T3 cells overexpressing IRS-1 were ... Establishment of NIH/3T3 cells with stable overexpression of functional IRS-1. (A) Wild-type NIH/3T3 cells were serum starved ... Establishment of NIH/3T3 cells overexpressing functional IRS-1 We chose NIH/3T3 cells as an experimental model to investigate ... we established NIH/3T3 cells with stable overexpression of IRS-1. The levels of total IRS-1 in both the control NIH/3T3 cells ...
more infohttp://www.biomedsearch.com/nih/Insulin-receptor-substrate-1-prevents/22788551.html
Transformation of NIH 3T3 cells by cotransfection with c-src and nuclear oncogenes. | Molecular and Cellular Biology  Transformation of NIH 3T3 cells by cotransfection with c-src and nuclear oncogenes. | Molecular and Cellular Biology
Transformation of NIH 3T3 cells by cotransfection with c-src and nuclear oncogenes.. D Shalloway, P J Johnson, E O Freed, D ... Transformation of NIH 3T3 cells by cotransfection with c-src and nuclear oncogenes. ... Transformation of NIH 3T3 cells by cotransfection with c-src and nuclear oncogenes. ... Transformation of NIH 3T3 cells by cotransfection with c-src and nuclear oncogenes. ...
more infohttps://mcb.asm.org/content/7/10/3582
Suppression of vinculin expression by antisense transfection confers changes in cell morphology, motility, and anchorage...  Suppression of vinculin expression by antisense transfection confers changes in cell morphology, motility, and anchorage...
J. Cell Biol. 119:427). To study the effect of reduced vinculin expression on cell behavior, 3T3 cells were transfected with an ... The expression of vinculin, a major component of adhesion plaques and cell-cell junctions, is markedly modulated in cells ... These cells showed a round phenotype with smaller and fewer vinculin-positive plaques localized mostly at the cell periphery. ... The results demonstrate that the regulation of vinculin expression in cells can affect, in a major way, cell shape and motility ...
more infohttp://jcb.rupress.org/content/122/6/1285?ijkey=d7b345cf00cb93d89a966ca198627f0254eaaf62&keytype2=tf_ipsecsha
AID 1290984 - Antitumor activity against mouse NIH/3T3 cells expressing IGF-1 receptor implanted in athymic nude mouse assessed...  AID 1290984 - Antitumor activity against mouse NIH/3T3 cells expressing IGF-1 receptor implanted in athymic nude mouse assessed...
Antitumor activity against mouse NIH/3T3 cells expressing IGF-1 receptor implanted in athymic nude mouse assessed as tumor ...
more infohttps://pubchem.ncbi.nlm.nih.gov/bioassay/1290984
Cellular Ras and Cyclin D1 Are Required during Different Cell Cycle Periods in Cycling NIH 3T3 Cells | Molecular and Cellular...  Cellular Ras and Cyclin D1 Are Required during Different Cell Cycle Periods in Cycling NIH 3T3 Cells | Molecular and Cellular...
Cell age and cell cycle position. Asynchronous NIH 3T3 cells were followed for 20 h in time lapse, with the last 30 min in the ... NIH 3T3 cells and reveal a sequence of signaling events required for cell cycle progression in proliferating NIH 3T3 cells. ... In a cell with a relatively short G1 phase, such as the NIH 3T3 cells studied here, Ras must become activated during G2phase to ... 1978) Exponential 3T3 cells escape in mid-G1 from their high serum requirement. Exp. Cell Res. 116:103-113. ...
more infohttps://mcb.asm.org/content/19/7/4623
Ecdysone-inducible expression of oncogenic Ha-Ras in NIH 3T3 cells leads to transient nuclear localization of activated...  Ecdysone-inducible expression of oncogenic Ha-Ras in NIH 3T3 cells leads to transient nuclear localization of activated...
Ecdysone-inducible expression of oncogenic Ha-Ras in NIH 3T3 cells leads to transient nuclear localization of activated ... Ecdysone-inducible expression of oncogenic Ha-Ras in NIH 3T3 cells leads to transient nuclear localization of activated ... Ecdysone-inducible expression of oncogenic Ha-Ras in NIH 3T3 cells leads to transient nuclear localization of activated ... Ecdysone-inducible expression of oncogenic Ha-Ras in NIH 3T3 cells leads to transient nuclear localization of activated ...
more infohttp://www.biochemj.org/content/362/2/305
Microscopy  -  NIH 3t3 cells  Microscopy - NIH 3t3 cells
Max Image from a series scan. Depth of section 6.56 µm. Nuclei are stained in blue, f-actin filaments in red and microtubule associated proteins in green ...
more infohttp://digitalcollections.stlawu.edu/collections/microscopy/nih-3t3-cells
Embryonic Swiss Mouse Fibroblast Cells (3T3 Line) | MicroscopyU  Embryonic Swiss Mouse Fibroblast Cells (3T3 Line) | MicroscopyU
The culture was counterstained for DNA in the cell nucleus with DAPI. Images were recorded in grayscale with a 12-bit digital ... The culture of embryonic Swiss mouse fibroblast cells presented in the digital image above was labeled with MitoTracker Red ... Embryonic Swiss Mouse Fibroblast Cells (3T3 Line). The culture of embryonic Swiss mouse fibroblast cells presented in the ... The culture was counterstained for DNA in the cell nucleus with DAPI. Images were recorded in grayscale with a 12-bit digital ...
more infohttps://www.microscopyu.com/gallery-images/embryonic-swiss-mouse-fibroblast-cells-3t3-line-4
  • In addition to inducing necrosis and apoptosis, ROS induces autophagic cell death. (biomedsearch.com)
  • Here we show that cotransfection, but not solo transfection, of expression plasmids for c-src and either adenovirus E1A, v-myc, c-myc, or the 5' half of polyomavirus large T (pltN) antigen into NIH 3T3 cells induces anchorage-independent growth, enhanced focus formation, and, for pltN cotransfection, tumorigenicity in adult NFS mice. (asm.org)
  • The BALB/3T3 mouse embryo cell line, noted for its marked postconfluence inhibition of proliferation, anchorage dependence, and high serum requirement, and frequently studied as a prototype nontumorigenic "fibroblast" line that is compared with tumorigenic sublines transformed with various agents, produced tumors within 2 to 3 months when an average of 3 × 10 4 cells were implanted s.c. attached to 1- × 5- × 10-mm polycarbonate platelets. (aacrjournals.org)
  • The tumors, as well as others arising from implants of BALB/3T3 cells attached to 3-mm glass beads, were given the histological diagnosis of "vasoformative saroma" because the tumor cells frequently formed vascular channels. (aacrjournals.org)
  • That the tumors were derived from BALB/3T3 cells and not host cells was proved when tumors arising in BALB/c × C57BL/6 F 1 hybrids were shown to be transplantable to BALB/c but not to C57BL/6 mice. (aacrjournals.org)
  • We conclude that BALB/3T3 cells are preneoplastic and give rise to different spontaneously transformed clones bearing unique tumor rejection antigens when implanted in vivo attached to a solid substrate. (aacrjournals.org)
  • The kinetics of acidification of diferric human transferrin in BALB/c mouse 3T3 cells were determined by flow cytometry using a modification of the fluorescein-rhodamine fluorescence ratio technique. (pnas.org)
  • We have compared the effects of ellipticine, several of its analogues, and two aza-analogue ellipticine derivatives (BD-40 and BR-1376) on cell cycle progression of BALB/c 3T3 mouse cells under different growth conditions. (aacrjournals.org)
  • Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. (wikipedia.org)
  • The cultured tumor cells showed loss of both postconfluence inhibition of proliferation and anchorage dependence. (aacrjournals.org)
  • In order to analyze whether some of the products in the mevalonic acid biosynthetic pathway may be of importance in the control of G 1 traverse and cell proliferation of SV-3T3 cells, cholesterol, Coenzyme Q, and dolichol were added as supplements to cells treated with 25-OH. (aacrjournals.org)
  • Indeed, since the overall length of the cell cycle appears to depend in large part on the length of the G 1 phase, it is likely that this cell cycle period must be compressed as much as possible for rapid proliferation, potentially requiring the transfer of activities present in the G 1 phase of quiescent cells to other cell cycle periods when they continuously cycle ( 26 ). (asm.org)
  • Not long after FGF1 and FGF2 were isolated, another group isolated a pair of heparin-binding growth factors that they named HBGF-1 and HBGF-2, while a third group isolated a pair of growth factors that caused proliferation of cells in a bioassay containing blood vessel endothelium cells, which they called ECGF1 and ECGF2. (wikipedia.org)
  • Independent of CDK, cyclin D1 binds to nuclear receptors (including estrogen receptor α, thyroid hormone receptor, PPARγ and AR ) to regulate cell proliferation, growth, and differentiation. (wikipedia.org)
  • Cyclin D1 also binds to histone acetylases and histone deacetylases to regulate cell proliferation and cell differentiation genes in the early to mid-G1 phase. (wikipedia.org)
  • Removing MDM2 simultaneously with the H2AK119 E3 ligase Ring1B/RNF2 further induced these genes and synthetically arrested cell proliferation. (wikipedia.org)
  • This transient refractory state was accompanied by a total conservation of long-term viability of these cells at least for the next 6 h following serum and drug addition. (aacrjournals.org)
  • Tumorigenic 3T3 cells maintain an alkaline intracellular pH under physiological conditions. (pnas.org)
  • Only the MBD2 cell lines were highly tumorigenic and invasive when injected into Nude mice while also showing a remarkable ability to invade and degrade bone tissue when injected into the tibia of SCID mice. (aacrjournals.org)
  • The Journal of Cell Biology. (wikipedia.org)
  • He has over 150 publications in journals, including Science, Nature, Proceedings of the National Academy of Sciences, USA, Molecular Cell Biology, and Journal of Cell Biology. (wikipedia.org)
  • This may result in differential responses by different cell types during exposure to tumor promoters. (aacrjournals.org)
  • Long-term viability of these cells was completely suppressed after a short exposure to the drugs. (aacrjournals.org)
  • Expressing TCTP in U2OS (Human Bone Osteosarcoma Epithelial Cells) protected them from cell death induced by etoposide over various concentrations and durations of exposure. (wikipedia.org)
  • In contrast, while ellipticine and its derivatives caused identical effects in cells recovering from serum starvation, BD-40 and BR-1376 did not block cells in G 2 phase and did not prevent the completion of the first division round occurring after serum addition to quiescent cells. (aacrjournals.org)
  • While results obtained with quiescent cells are important, there is reason to believe the situation might be different for cells continuously progressing through the cell cycle. (asm.org)
  • The suffix "-blast" is used in cellular biology to denote a stem cell or a cell in an activated state of metabolism. (wikipedia.org)
  • these saw that a 20% reduction in the amount of water in a cell inhibits metabolism, with metabolism decreasing progressively as the cell dries out and all metabolic activity halting when the water level reaches 70% below normal. (wikipedia.org)
  • Cell Metabolism. (wikipedia.org)
  • 3T3-F4424 cells when implanted into an athymic (nude) mice gave rise to fat pads that were similar to endogenous white adipose tissue. (wikipedia.org)
  • TCTP knockdown in primary mammary tumor cells, results in increased p53 expression and a decreased number of stem-like cancer cells. (wikipedia.org)
  • Elevated phosphocholine concentration in ras-transformed NIH 3T3 cells arises from increased choline kinase activity, not from phosphatidylcholine breakdown. (asm.org)
  • It was suggested that the phosphocholine arises from constitutive hydrolysis of phosphatidylcholine by phospholipase C, an activity that would also account for the elevated 1,2-diacylglycerol found in ras-transformed cells. (asm.org)
  • No increased breakdown of phosphatidylcholine was observed in ras-transformed cells. (asm.org)
  • Cell viability, cell apoptosis, reactive oxygen species (ROS) level, lactate dehydrogenase (LDH) assay, the level of glutathione (GSH), activities of superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) level were detected. (mdpi.com)
  • Specifically, "3T3-L1" is one of the current lines. (wikipedia.org)
  • Moreover, the effect is not specifically a function of the presence of the viral genome, but is a reflection of the overall growth rate and physiological state of the cell. (sciencemag.org)
  • 1233-1239, 1986) that 25-OH inhibits the cell cycle traverse of SV-3T3 cells specifically in early G 1 . (aacrjournals.org)
  • However, when the ability of all these compounds to kill tumor cells in cell culture was investigated, it turned out that the antitumor potency did not at all depend on whether or not the respective compound could inhibit COX-2, showing that inhibition of COX-2 was not required for the anticancer effects. (wikipedia.org)
  • In untreated control cells, most of this activity found with the nuclear fraction can be extracted by chelators. (aacrjournals.org)
  • Autophagy usually serves as a survival mechanism in response to stress conditions, but excessive induction of autophagy results in cell death. (biomedsearch.com)