An enzyme of the shikimate pathway of AROMATIC AMINO ACID biosynthesis, it generates 5-enolpyruvylshikimate 3-phosphate and ORTHOPHOSPHATE from PHOSPHOENOLPYRUVATE and shikimate-3-phosphate. The shikimate pathway is present in BACTERIA and PLANTS but not in MAMMALS.
A somewhat heterogeneous class of enzymes that catalyze the transfer of alkyl or related groups (excluding methyl groups). EC 2.5.
A species of gram-positive, aerobic bacteria that produces TUBERCULOSIS in humans, other primates, CATTLE; DOGS; and some other animals which have contact with humans. Growth tends to be in serpentine, cordlike masses in which the bacilli show a parallel orientation.
A tri-hydroxy cyclohexene carboxylic acid important in biosynthesis of so many compounds that the shikimate pathway is named after it.
Any of the infectious diseases of man and other animals caused by species of MYCOBACTERIUM.
MYCOBACTERIUM infections of the lung.
Drugs used in the treatment of tuberculosis. They are divided into two main classes: "first-line" agents, those with the greatest efficacy and acceptable degrees of toxicity used successfully in the great majority of cases; and "second-line" drugs used in drug-resistant cases or those in which some other patient-related condition has compromised the effectiveness of primary therapy.
A species of gram-negative bacteria that grows preferentially in the vacuoles of the host cell. It is the etiological agent of Q FEVER.
A genus of gram-negative, rod-shaped bacteria that is widely distributed in TICKS and various mammals throughout the world. Infection with this genus is particularly prevalent in CATTLE; SHEEP; and GOATS.
An acute infectious disease caused by COXIELLA BURNETII. It is characterized by a sudden onset of FEVER; HEADACHE; malaise; and weakness. In humans, it is commonly contracted by inhalation of infected dusts derived from infected domestic animals (ANIMALS, DOMESTIC).
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A species of gram-negative bacteria, in the family Aeromonadaceae. It is strictly parasitic and often pathogenic causing FURUNCULOSIS in SALMONIDS and ulcer disease in GOLDFISH.
A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that occurs singly, in pairs, or in short chains. Its organisms are found in fresh water and sewage and are pathogenic to humans, frogs, and fish.
A persistent skin infection marked by the presence of furuncles, often chronic and recurrent. In humans, the causative agent is various species of STAPHYLOCOCCUS. In salmonid fish (SALMONIDS), the pathogen is AEROMONAS SALMONICIDA.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria that may be pathogenic for frogs, fish, and mammals, including man. In humans, cellulitis and diarrhea can result from infection with this organism.
Diseases of freshwater, marine, hatchery or aquarium fish. This term includes diseases of both teleosts (true fish) and elasmobranchs (sharks, rays and skates).
The degree of 3-dimensional shape similarity between proteins. It can be an indication of distant AMINO ACID SEQUENCE HOMOLOGY and used for rational DRUG DESIGN.
Macromolecular molds for the synthesis of complementary macromolecules, as in DNA REPLICATION; GENETIC TRANSCRIPTION of DNA to RNA, and GENETIC TRANSLATION of RNA into POLYPEPTIDES.
The process of pictorial communication, between human and computers, in which the computer input and output have the form of charts, drawings, or other appropriate pictorial representation.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The portion of an interactive computer program that issues messages to and receives commands from a user.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria that is extremely pathogenic and causes severe dysentery. Infection with this organism often leads to ulceration of the intestinal epithelium.
A species of gram-negative bacteria (currently incertae sedis) causing multisystem disease in CATTLE.
Amino acids containing an aromatic side chain.
Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.
A species of bacteria that causes ANTHRAX in humans and animals.
A species of gram-negative, aerobic bacteria primarily found in purulent venereal discharges. It is the causative agent of GONORRHEA.
Proteins synthesized by organisms belonging to the phylum ARTHROPODA. Included in this heading are proteins from the subdivisions ARACHNIDA; CRUSTACEA; and HORSESHOE CRABS. Note that a separate heading for INSECT PROTEINS is listed under this heading.
Antigens from the house dust mites (DERMATOPHAGOIDES), mainly D. farinae and D. pteronyssinus. They are proteins, found in mite feces or mite extracts, that can cause ASTHMA and other allergic diseases such as perennial rhinitis (RHINITIS, ALLERGIC, PERENNIAL) and atopic dermatitis (DERMATITIS, ATOPIC). More than 11 groups of Dermatophagoides ALLERGENS have been defined. Group I allergens, such as Der f I and Der p I from the above two species, are among the strongest mite immunogens in humans.
Any arthropod of the subclass ACARI except the TICKS. They are minute animals related to the spiders, usually having transparent or semitransparent bodies. They may be parasitic on humans and domestic animals, producing various irritations of the skin (MITE INFESTATIONS). Many mite species are important to human and veterinary medicine as both parasite and vector. Mites also infest plants.
Species of American house dust mite, in the family PYROGLYPHIDAE.
An enzyme catalyzing the oxidation of 2 moles of glutathione in the presence of hydrogen peroxide to yield oxidized glutathione and water. EC 1.11.1.9.
Small proteinaceous infectious particles which resist inactivation by procedures that modify NUCLEIC ACIDS and contain an abnormal isoform of a cellular protein which is a major and necessary component. The abnormal (scrapie) isoform is PrPSc (PRPSC PROTEINS) and the cellular isoform PrPC (PRPC PROTEINS). The primary amino acid sequence of the two isoforms is identical. Human diseases caused by prions include CREUTZFELDT-JAKOB SYNDROME; GERSTMANN-STRAUSSLER SYNDROME; and INSOMNIA, FATAL FAMILIAL.
The systematic study of the complete DNA sequences (GENOME) of organisms.
Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)
Inorganic salts of phosphoric acid.
Substances or mixtures that are added to the soil to supply nutrients or to make available nutrients already present in the soil, in order to increase plant growth and productivity.
Acetic acid derivatives of the heterocyclic compound indole. (Merck Index, 11th ed)
A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.
Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments.

Molecular cloning and sequencing of the aroA gene from Actinobacillus pleuropneumoniae and its use in a PCR assay for rapid identification. (1/105)

The gene (aroA) of Actinobacillus pleuropneumoniae, serotype 2, encoding 5-enolpyruvylshikimate-3-phosphate synthase was cloned by complementation of the aroA mutation in Escherichia coli K-12 strain AB2829, and the nucleotide sequence was determined. A pair of primers from the 5' and 3' termini were selected to be the basis for development of a specific PCR assay. A DNA fragment of 1,025 bp was amplified from lysed A. pleuropneumoniae serotypes 1 to 12 of biovar 1 or from isolated DNA. No PCR products were detected when chromosomal DNAs from other genera were used as target DNAs; however, a 1,025-bp DNA fragment was amplified when Actinobacillus equuli chromosomal DNA was used as a target, which could be easily differentiated by its NAD independence. The PCR assay developed was very sensitive, with lower detection limits of 12 CFU with A. pleuropneumoniae cells and 0.8 pg with extracted DNA. Specificity and sensitivity make this PCR assay a useful method for the rapid identification and diagnosis of A. pleuropneumoniae infections.  (+info)

Evaluation of Salmonella typhimurium mutants in a model of experimental gastroenteritis. (2/105)

Salmonella typhimurium strains harboring independent, defined mutations in aroA, invA, ssrA, or msbB were assessed for their ability to induce fluid accumulation, tissue damage, and local inflammation in rabbit ileal loops. Three wild-type strains of S. typhimurium, TML, HWSH, and SL1344, and two mutant strains, S. typhimurium SL1344 ssrA and S. typhimurium SL1344 msbB, consistently induced fluid accumulation in the lumen of loops and inflammation of loop-associated tissues. In contrast, three different S. typhimurium aroA strains and an invA mutant of SL1344 did not induce significant fluid accumulation in the rabbit ileal loops. However, the S. typhimurium aroA strains did induce an inflammatory infiltrate and some local villus-associated damage, but the invA mutant did not. Histologically, wild-type S. typhimurium, S. typhimurium SL1344 ssrA, and S. typhimurium SL1344 msbB demonstrated more severe effects on villus architecture than S. typhimurium aroA strains, whereas S. typhimurium invA-infected loops showed no detectable damage. This suggests that villus damage most likely contributes to fluid accumulation within the loop.  (+info)

Characterization of Streptococcus pneumoniae 5-enolpyruvylshikimate 3-phosphate synthase and its activation by univalent cations. (3/105)

The aroA gene (Escherichia coli nomenclature) encoding 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase from the gram-positive pathogen Streptococcus pneumoniae has been identified, cloned and overexpressed in E. coli, and the enzyme purified to homogeneity. It was shown to catalyze a reversible conversion of shikimate 3-phosphate (S3P) and phosphoenolpyruvate (PEP) to EPSP and inorganic phosphate. Activation by univalent cations was observed in the forward reaction, with NH+4, Rb+ and K+ exerting the greatest effects. Km(PEP) was lowered by increasing [NH+4] and [K+], whereas Km(S3P) rose with increasing [K+], but fell with increasing [NH+4]. Increasing [NH+4] and [K+] resulted in an overall increase in kcat. Glyphosate (GLP) was found to be a competitive inhibitor with PEP, but the potency of inhibition was profoundly affected by [NH+4] and [K+]. For example, increasing [NH+4] and [K+] reduced Ki(GLP versus PEP) up to 600-fold. In the reverse reaction, the enzyme catalysis was less sensitive to univalent cations. Our analysis included univalent cation concentrations comparable with those found in bacterial cells. Therefore, the observed effects of these metal ions are more likely to reflect the physiological behavior of EPSP synthase and also add to our understanding of how to inhibit this enzyme in the host organism. As there is a much evidence to suggest that EPSP synthase is essential for bacterial survival, its discovery in the serious gram-positive pathogen S. pneumoniae and its inhibition by GLP indicate its potential as a broad-spectrum antibacterial target.  (+info)

Extending the CD4(+) T-cell epitope specificity of the Th1 immune response to an antigen using a Salmonella enterica serovar typhimurium delivery vehicle. (4/105)

We analyzed the CD4 T-cell immunodominance of the response to a model antigen (Ag), MalE, when delivered by an attenuated strain of Salmonella enterica serovar Typhimurium (SL3261*pMalE). Compared to purified MalE Ag administered with adjuvant, the mapping of the peptide-specific proliferative responses showed qualitative differences when we used the Salmonella vehicle. We observed the disappearance of one out of eight MalE peptides' T-cell reactivity upon SL3261*pMalE immunization, but this phenomenon was probably due to a low level of T-cell priming, since it could be overcome by further immunization. The most striking effect of SL3261*pMalE administration was the activation and stimulation of new MalE peptide-specific T-cell responses that were silent after administration of purified Ag with adjuvant. Ag presentation assays performed with MalE-specific T-cell hybridomas showed that infection of Ag-presenting cells by this intracellular attenuated bacterium did not affect the processing and presentation of the different MalE peptides by major histocompatibility complex (MHC) class II molecules and therefore did not account for immunodominance modulation. Thus, immunodominance of the T-cell response to microorganisms is governed not only by the frequency of the available T-cell repertoire or the processing steps in Ag-presenting cells that lead to MHC presentation but also by other parameters probably related to the infectious process and to the bacterial products. Our results indicate that, upon infection by a microorganism, the specificity of the T-cell response induced against its Ags can be much more effective than with purified Ags and that it cannot completely be mimicked by purified Ags administered with adjuvant.  (+info)

Comparison of abilities of Salmonella enterica serovar typhimurium aroA aroD and aroA htrA mutants to act as live vectors. (5/105)

We compared the ability of Salmonella enterica serovar Typhimurium SL1344 aroA aroD (BRD509) and aroA htrA (BRD807) mutants to act as live vectors for delivery of fragment C of tetanus toxin (FrgC). FrgC was expressed in these strains from either pTETnir15 or pTEThtrA1. BRD509FrgC(+) strains elicited approximately 2-log-higher serum anti-FrgC antibody titers than BRD807FrgC(+) strains. All mice immunized with BRD807pTEThtrA1, BRD509pTEThtrA1, and BRD509pTETnir15 (but not BRD807pTETnir15) were protected against tetanus.  (+info)

Interaction of the herbicide glyphosate with its target enzyme 5-enolpyruvylshikimate 3-phosphate synthase in atomic detail. (6/105)

Biosynthesis of aromatic amino acids in plants, many bacteria, and microbes relies on the enzyme 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase, a prime target for drugs and herbicides. We have identified the interaction of EPSP synthase with one of its two substrates (shikimate 3-phosphate) and with the widely used herbicide glyphosate by x-ray crystallography. The two-domain enzyme closes on ligand binding, thereby forming the active site in the interdomain cleft. Glyphosate appears to occupy the binding site of the second substrate of EPSP synthase (phosphoenol pyruvate), mimicking an intermediate state of the ternary enzyme.substrates complex. The elucidation of the active site of EPSP synthase and especially of the binding pattern of glyphosate provides a valuable roadmap for engineering new herbicides and herbicide-resistant crops, as well as new antibiotic and antiparasitic drugs.  (+info)

Genetic background of attenuated Salmonella typhimurium has profound influence on infection and cytokine patterns in human dendritic cells. (7/105)

Salmonella typhimurium (ST) can cause infection in man, and attenuated strains are under consideration as live vaccine vectors. However, little is known about the interaction of ST with human dendritic cells (DC). Here, we compared the consequences of exposure of human, monocyte-derived DC with different attenuated strains of ST. Infection was observed with all four strains tested (wild type, PhoP-, PhoPc, and AroA), but the PhoPc strain was by far the most efficient. Intracellular persistence of wild type and PhoP- was longer than that of PhoPc and AroA, both of which were largely eliminated within 24 h. Most DC survived infection by the attenuated strains, although apoptosis was observed in a fraction of the exposed cells. All strains induced DC maturation, independent from the extent of infection. Although all strains stimulated secretion of TNF-alpha and IL-12 strongly, PhoPc induced significantly less IL-10 than the other three strains and as much as 10 times less IL-10 than heat-killed PhoPc, suggesting that this mutant suppressed the secretion of IL-10 by the DC. These data indicate that infectivity, bacterial elimination, and cytokine secretion in human DC are controlled by the genetic background of ST.  (+info)

Chemical shift mapping of shikimate-3-phosphate binding to the isolated N-terminal domain of 5-enolpyruvylshikimate-3-phosphate synthase. (8/105)

To facilitate evaluation of enzyme-ligand complexes in solution, we have isolated the 26-kDa N-terminal domain of 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase for analysis by NMR spectroscopy. The isolated domain is capable of binding the substrate shikimate-3-phosphate (S3P), and this letter reports the localization of the S3P binding site using chemical shift mapping. Based on the NMR data, we propose that Ser23, Arg27, Ser197, and Tyr200 are directly involved in S3P binding. We also describe changes in the observed nuclear Overhauser effects (NOEs) that are consistent with a partial conformational change in the N-terminal domain upon S3P binding.  (+info)

5-enolpyruvylshikimate-3-phosphate (EPSP) synthase is an enzyme produced by plants and microorganisms. It catalyzes the chemical reaction: phosphoenolpyruvate + 3-phosphoshikimate ⇌ {\displaystyle \rightleftharpoons } phosphate + 5-enolpyruvylshikimate-3-phosphate (EPSP) Thus, the two substrates of this enzyme are phosphoenolpyruvate (PEP) and 3-phospho-shikimate, whereas its two products are phosphate and 5-enolpyruvylshikimate-3-phosphate. It is the biological target of the herbicide glyphosate, and a glyphosate-resistant version of this gene has been used in genetically modified crops. The enzyme belongs to the family of transferases, to be specific those transferring aryl or alkyl groups other than methyl groups. The systematic name of this enzyme class is phosphoenolpyruvate:3-phosphoshikimate 5-O-(1-carboxyvinyl)-transferase. Other names in common use include: 5-enolpyruvylshikimate-3-phosphate synthase, 3-enolpyruvylshikimate 5-phosphate synthase, 3-enolpyruvylshikimic acid-5-phosphate ...
Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.
THIRD WORLD NETWORK BIOSAFETY INFORMATION SERVICE. Dear Friends and Colleagues. Glyphosate-Resistant Weeds A Growing Major Problem Worldwide. Glyphosate-resistant soybean was introduced in 1996, the first of several (genetically modified) glyphosate-resistant crops, which allowed the herbicide, glyphosate, to be used as a selective post-emergence herbicide. Glyphosates widespread use has resulted in a situation where glyphosate-resistant weed species are evolving at a steady pace and the more virulent existing glyphosate-resistant weeds are spreading rapidly.. A recent review (Item 1) gives us an overview of the incidence of glyphosate-resistant weeds in the world. Thirty-eight weed species have now evolved resistance to glyphosate, distributed across 37 countries and in 34 different crops and six non-crop situations. Glyphosate-resistant weeds in glyphosate-resistant crop systems account for more than 90% of the area infested and the economic damage caused by glyphosate-resistant weeds ...
Controversy over the herbicide glyphosate has been raging for years in Europe but the plant protection product has neither been subject to an outright ban or given the all-clear. Katia Merten-Lentz of international law firm Keller and Heckman explains how glyphosate found itself in limbo.
Buy aroA recombinant protein, 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) Recombinant Protein-YP_001088340.1 (MBS1290666) product datasheet at MyBioSource, Recombinant Proteins
Article Dynamics and environmental risk assessment of the herbicide glyphosate and its metabolite AMPA in a small vineyard river of the Lake Geneva catchment. The use of pesticides may lead to environmental problems, such as surface water pollution, ...
1G6S: Interaction of the herbicide glyphosate with its target enzyme 5-enolpyruvylshikimate 3-phosphate synthase in atomic detail.
1G6T: Interaction of the herbicide glyphosate with its target enzyme 5-enolpyruvylshikimate 3-phosphate synthase in atomic detail.
Given the importance of soybean to the global economy and as a food source, improving crop management techniques is integral to obtaining higher yields. As such, this study aimed to assess the yield of soybean cultivars as a function of different glyphosate salt formulations, doses and application times. Two field experiments were conducted simultaneously using a randomized block design and 3x4x2 factorial scheme, with three repetitions. Factor A consisted of three glyphosate formulations: isopropylamine salt (Roundup Original®), ammonium salt (Roundup WG®), and potassium salt (Zapp Qi®), factor B four application times: 0, 14, 28, 42 days after planting (DAP), and factor C two doses: the recommended dose of 720 grams of acid equivalent per hectare and twice that (1440 grams of acid equivalent per hectare). There was no difference between the glyphosate salts for the BMX Ativa RR® cultivar, but yields of BMX Apolo RR® submitted to ammonium salt were 28% higher than those recorded for the ...
An fragrant amino acid referred to as EPSP synthase is a vital enzyme that just about absolutely will get disrupted by glyphosate by this mechanism of substituting for glycine. This will get a bit technical, however it will be significant. The plant model of EPSP synthase binds a phosphate group in its substrate phosphoenolpyruvate at a web site the place theres a highly-conserved glycine residue (extremely conserved normally implies that its vital for correct operate). It has been proven experimentally that, in the event you change the DNA code in order that the glycine is substituted by an amino acid referred to as alanine (one additional methyl group), the enzyme turns into fully insensitive to glyphosate at any focus. It additionally takes a success on phosphate binding as a result of of the additional methyl group, however you may tweak one other amino acid close by to repair this drawback, whereas nonetheless conserving its insensitivity to glyphosate. Researchers from Dow-Dupont did ...
Gyphosate is a commonly used nonselective herbicide that inhibits plant growth through interference with the production of essential aromatic amino acids. In vivo studies in mammals with radiolabeled glyphosate have shown that 34% of radioactivity was associated with intestinal tissue 2 h after oral administration. The aim of our research was to investigate the transport, binding, and toxicity of glyphosate to the cultured human intestinal epithelial cell line, Caco‐2, and the rat small intestinal crypt‐derived cell line, ileum epithelial cells‐18 (IEC‐18 ). An in vitro analysis of the transport kinetics of [14C]‐glyphosate showed that 4 h after exposure, approximately 8% of radiolabeled glyphosate moved through the Caco‐2 monolayer in a dose‐dependent manner. Binding of glyphosate to cells was saturable and approximately 4 1011 binding sites/cell were estimated from bound [14C]. Exposure of Caco‐2 cells to ≥10 mg/ml glyphosate reduced transmembrane electrical resistance (TEER) ...
Gyphosate is a commonly used nonselective herbicide that inhibits plant growth through interference with the production of essential aromatic amino acids. In vivo studies in mammals with radiolabeled glyphosate have shown that 34% of radioactivity was associated with intestinal tissue 2 h after oral administration. The aim of our research was to investigate the transport, binding, and toxicity of glyphosate to the cultured human intestinal epithelial cell line, Caco‐2, and the rat small intestinal crypt‐derived cell line, ileum epithelial cells‐18 (IEC‐18 ). An in vitro analysis of the transport kinetics of [14C]‐glyphosate showed that 4 h after exposure, approximately 8% of radiolabeled glyphosate moved through the Caco‐2 monolayer in a dose‐dependent manner. Binding of glyphosate to cells was saturable and approximately 4 1011 binding sites/cell were estimated from bound [14C]. Exposure of Caco‐2 cells to ≥10 mg/ml glyphosate reduced transmembrane electrical resistance (TEER) ...
Health Canada has notified Stine Seed Farm, Inc. that it has no objection to the food use of corn expressing a version of corn 5-enolpyruvylshikimate-3-phosphate synthase to confer tolerance to glyphosate herbicide. The Department conducted a comprehensive assessment of this corn event according to its Guidelines for the Safety Assessment of Novel Foods.
Many people are concerned that pesticides, in particular, glyphosate, alter the gut microbiome and somehow cause autism. […] We can calculate how much glyphosate you would ingest on average from one piece of produce and see if that is enough to kill bacteria. So if you eat a 5 ounce (~150 g) piece of vegetable treated with glyphosate (5 ounces is about the size of an average apple for reference). Using his [Credible Hulks] high estimate of 5 ppm [glyphosate residue], we calculate that you get 0.75 mg of glyphosate. 98% is not absorbed so lets assume it stays in your stomach for as long as it takes to pass through your digestive system. The 2% lost is not enough to worry about for this estimate. So 0.75 mg in 1 L (the volume of a full stomach) is 4.4 uM (micromolar). The papers I found looking at toxicity of glyphosate in bacteria were using MILLIMOLAR range concentrations of glyphosate. Micromolar means 10e-6 molar, or 0.001 Millimolar. So 4.4 micromolar is 4.4 x 10e-6 Molar or 0.0044 ...
The parental MON 89034 has the expression cassettes of genes Cry1A.105 and cry2Ab 2 (derived from Bacillus thuringiensis), which encode the proteins Cry1A.105 and Cry2Ab2, respectively, responsible for insect resistance. The parental NK 603 maize contains two expression cassettes of the cp4 epsps gene (derived from Agrobacterium sp. CP4 strain), with the respective regulatory sequence. The cp4 epsps gene encodes the protein 5-enolpyruvyl-shikimate-3-phosphate synthesis (CP4 EPSPS), conferring tolerance to the herbicide glyphosate. The pyramided parental maize MON 89034 x NK 603 has been extensively tested, and its biosafety been examined in separate cases, both approved by CTNBio. The proteins present in NK 603 x MON89034 maize have a history of safe use by the consumption of MON 89034 maize since 2008, and the NK603 maize since 2000. The MON 89034 x NK 603 maize is approved in Japan, North Korea, the Philippines, Taiwan, and the USA. Concerning the proteins expressed by MON 89034 x NK 603 ...
A weed resistant to a widely used chemical to protect crops has spread for the first time to Western Canada, the countrys grain and canola belt.
Unter NoTill entfällt das Pflügen als unkrautkontrollierende Maßnahme, daher beruht hier die Unkrautbekämpfung aktuell vor allem auf dem Einsatz von Herbiziden.,ref,Reddy, P. (Hrsg.; 2017): Agro-ecological Approaches to Pest Management for Sustainable Agriculture. Springer Nature. Singapur.,/ref, Das Totalherbizid Glyphosat bzw. N(Phosphonomethyl)glycin wurde in den letzten 40 Jahren in großem Umfang eingesetzt.,ref name=:8,Van Bruggen, A., Shin, K., Mai, V., Jeong, K., Finckh, M., Morris, J. (2018): Environmental and health effects of the herbicide glyphosate. Science of the Total Environment, 616, 255-268.,/ref, Auch wenn die akute Toxidität von Glyphosat auf Säugetiere gering ist, ist die Toxizität formulierter, glyphosathaltiger Herbizide häufig deutlich höher.,ref name=:8 /,,ref name=:9,Fortes, C., Mastroeni, S., Segatto, M., Hohmann, C., Miligi, L., Bakos, L., Bonamigo, R. (2016): Occupational exposure to pesticides with occupational sun exposure increases the risk for ...
Furthermore, the document itself admits that control data for carcinomas, adenomas, hyperplasia, and other carcinogenic responses in the rats was provided by none other than MONSANTO.. Furthermore, as Sustainable Pulse points out, the Class C designation for glyphosate was changed by the EPA six years later to a Class E category which suggests evidence of non-carcinogenicity for humans. Mysteriously, this change in glyphosates classification occurred during the same period that Monsanto was developing its first Roundup-Ready (glyphosate-resistant) GM crops.. Is it any wonder it has taken so long for a large body with power to state that glyphosate and the other toxic chemicals that Monsanto makes are killing us?. The good news is that the world is now taking action against glyphosate and Monsanto at large.. ...
Mannose tolerance Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glyphosate Selectable marker genes and reporter ...
This study aimed to assess the water quality of the Perequê River, Porto Belo, Santa Catarina, Brazil, through a biomarker approach in the native fish species Geophagus brasiliensis, and the sediment chemical quantification of the herbicides glyphosate and bentazone used in irrigated rice plantations. This river is used for the public water supply of two municipalities. The first sampling (S1) was ...
SWISS-MODEL Repository entry for C4ZQ34 (AROA_ECOBW), 3-phosphoshikimate 1-carboxyvinyltransferase. Escherichia coli (strain K12 / MC4100 / BW2952)
common questions, predictably, is how to kill glyphosate-resistant marestail and giant ragweed and ... to kill in May, so this is an issue primarily in fields not treated last fall. The good news is that ... The bad news is that nothing we suggest here is going to be infallible on large marestail. A burndown .... ...
SWISS-MODEL Template Library (SMTL) entry for 2o0b.1. Mycobacterium tuberculosis epsp synthase in complex with S3P (partially photolyzed)
Many GMO critics believe that vaccines are contaminated with glyphosate. A look at how vaccines are made and how glyphosate is metabolized shows this hypothesis is flawed.
This German based new study concludes that Glyphosate residue could reach humans and animals through feed and excreted in urine. Presence of glyphosate in urine and its accumulation in animal tissues is alarming even at low concentrations.
by Robert Arnason Courtesy The Western Producer In a paper published online in Critical Reviews in Toxicology, four independent panels of experts looked at the relevant research on glyphosate and whether its carcinogenic. The group of 16 scientists, from Canada, the United States, Denmark, Brazil and the United Kingdom and other countries, decisively concluded that glyphosate is […]
Article Nanofiltration process of glyphosate simulated wastewater. Nanofiltration separation of glyphosate simulated wastewater was investigated using a DK membrane. The effects of operating parameters and the addition of impurities on membrane perfo...
Such microbes, says Zechel, are starved for phosphate. Often referred to as the staff of life, phosphate is critical to the chemical backbone of both DNA and RNA, not to mention the energy-carrying molecule adenosine triphosphate and the phospholipids that make up all cell membranes. So its not surprising that various organisms have evolved mechanisms for turning phosphonic acids - including glyphosate - into inorganic phosphate. The trick for scientists, therefore, is to harness this ability found in nature to reduce the potential impact of glyphosate in areas where it might have accumulated. Such a strategy would be preferable to an outright glyphosate ban, says Zechel, which could result in the creation of something far worse taking its place, simply because its so hard to improve on its mechanism of action, particularly in combination with genetically modified plants. Zechel describes the two-stage decomposition of glyphosate. First, microorganisms in the soil split the glyphosate ...
Under the control of Trump and Wheeler, the agency is virtually incapable of taking steps to protect people from dangerous chemicals like glyphosate.
This topic contains 78 study abstracts on Glyphosate indicating it may contribute to Lymphoma: Non-Hodgkin, DNA damage, and Hormonal Disorders: Children
This topic contains 78 study abstracts on Glyphosate indicating it may contribute to Lymphoma: Non-Hodgkin, DNA damage, and Hormonal Disorders: Children
This bombshell is something no one was expecting to come to light. Monsanto knew about the glyphosate link to cancer for more than 35 years!!! Research Scientist Dr.
Researcher finds a Monsanto document that reveals theyve known for 30 years that glyphosate can cause adenomas and carcinomas in the rats that theyve studied.
TY - CONF. T1 - INTRODUCTION AND EXPRESSION OF A GENE CONFERRING TOLERANCE TO THE HERBICIDE GLYPHOSATE IN POPULUS NC5339.. AU - Fillatti, J. J.. AU - McCown, B. H.. AU - Sellmer, James C.. AU - Haissig, B.. PY - 1986/12/1. Y1 - 1986/12/1. N2 - We have developed a plant regeneration and gene transfer system for Populus NC5339, using Agrobacterium tumefaciens as a vector. Employing this system we introduced a gene for glyphosate tolerance (aroA) into Populus NC5339 plants. This paper describes the strategy employed to achieve this goal.. AB - We have developed a plant regeneration and gene transfer system for Populus NC5339, using Agrobacterium tumefaciens as a vector. Employing this system we introduced a gene for glyphosate tolerance (aroA) into Populus NC5339 plants. This paper describes the strategy employed to achieve this goal.. UR - http://www.scopus.com/inward/record.url?scp=0022934435&partnerID=8YFLogxK. UR - ...
Glyphosate is the most important and widely used herbicide in world agriculture. Intensive glyphosate selection has resulted in the widespread evolution of glyphosate-resistant weed populations, threatening the sustainability of this valuable once-in-a-century agrochemical. Field-evolved glyphosate resistance due to known resistance mechanisms is generally low to modest. Here, working with a highly glyphosate-resistant Eleusine indica population, we identified a double amino acid substitution (T102I + P106S [TIPS]) in the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene in glyphosate-resistant individuals. This TIPSmutation recreates the biotechnology-engineered commercial first generation glyphosate-tolerant EPSPS in corn (Zea mays) and now in other crops. In E. indica, the naturally evolved TIPS mutants are highly (more than 180-fold) resistant to glyphosate compared with the wild type and more resistant (more than 32-fold) than the previously known P106S mutants. The E. indica TIPS ...
Glyphosate is the most important and widely used herbicide in world agriculture. Intensive glyphosate selection has resulted in the widespread evolution of glyphosate-resistant weed populations, threatening the sustainability of this valuable once-in-a-century agrochemical. Field-evolved glyphosate resistance due to known resistance mechanisms is generally low to modest. Here, working with a highly glyphosate-resistant Eleusine indica population, we identified a double amino acid substitution (T102I + P106S [TIPS]) in the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene in glyphosate-resistant individuals. This TIPSmutation recreates the biotechnology-engineered commercial first generation glyphosate-tolerant EPSPS in corn (Zea mays) and now in other crops. In E. indica, the naturally evolved TIPS mutants are highly (more than 180-fold) resistant to glyphosate compared with the wild type and more resistant (more than 32-fold) than the previously known P106S mutants. The E. indica TIPS ...
ROSO, A.C. and VIDAL, R.A.. A Modified phosphate-carrier protein theory is proposed as a non-target site mechanism For glyphosate resistance in weeds. Planta daninha [online]. 2010, vol.28, n.spe, pp.1175-1185. ISSN 0100-8358. http://dx.doi.org/10.1590/S0100-83582010000500025.. Glyphosate is an herbicide that inhibits the enzyme 5-enolpyruvyl-shikimate-3-phosphate synthase (EPSPs) (EC 2.5.1.19). EPSPs is the sixth enzyme of the shikimate pathway, by which plants synthesize the aromatic amino acids phenylalanine, tyrosine, and tryptophan and many compounds used in secondary metabolism pathways. About fifteen years ago it was hypothesized that it was unlikely weeds would evolve resistance to this herbicide because of the limited degree of glyphosate metabolism observed in plants, the low resistance level attained to EPSPs gene overexpression, and because of the lower fitness in plants with an altered EPSPs enzyme. However, today 20 weed species have been described with glyphosate resistant ...
A Saskatchewan study examined hybridization between two mustard (Brassica juncea and B. carinata) crops that were either adjacent to a glyphosate-resistant canola (B. napus) crop or separated by a 5-m strip. Overall, field hybridization levels, detected with glyphosate resistance and species-specific AFLP markers, were low: 0.024% and up to 400 m in the adjacent B. juncea field and 0.013% (up to 350 m) in the separated field, and 0.005% (up to 150 m) in the adjacent B. carinata field and 0.002% (up to 65 m) in the separated field. Based on fitness information under controlled conditions, the fertility of hybrid plants is expected to be low. ...
The aim of this study was cloning of wheat (Triticum aestivum L. cv. Bayraktar) Trehalose-6-phosphate synthase gene and examining of gene expression pattern of wheat seedlings in response to salt and drought stress conditions using Wheat GeneChip (Affymetrix). In this study, 10-days old wheat seedlings were subjected to the salt (350 mM NaCl) and drought stress (20% PEG) for 24 hours, then root and leaf tissues were used for wheat TPS gene cloning and microarray studies. RACE (Rapid Amplification of cDNA Ends) was used to determine cDNA sequence of wheat TPS gene, TaTPS. The ORF of TaTPS encodes a putative protein of 859 amino acids with a predicted molecular weight (MW) of 96.7 kDa and an isoelectric point (pI) of 5.97. Based on tblastx, TaTPS showed great similarity with other plants TPS genes. In root tissue, expression of TaTPS increased under drought stress while no change was observed under salt stress. In leaf tissue, both salt and drought treatments repressed the expression of TaTPS. ...
0136]B. Glyphosate (resistance conferred by mutant 5-enolpyruvlshikimate-3-phosphate synthase (EPSPS) and aroA genes, respectively) and other phosphono compounds such as glufosinate (phosphinothricin acetyl transferase (PAT) and Streptomyces hygroscopicus PAT bar genes), and pyridinoxy or phenoxy proprionic acids and cyclohexanediones (ACCase inhibitor-encoding genes). See, for example, U.S. Pat. No. 4,940,835 to Shah, et al., which discloses the nucleotide sequence of a form of EPSPS which can confer glyphosate resistance. U.S. Pat. No. 5,627,061 to Barry et al. also describes genes encoding EPSPS enzymes. See also U.S. Pat. Nos. 6,566,587; 6,338,961; 6,248,876 B1; 6,040,497; 5,804,425; 5,633,435; 5,145,783; 4,971,908; 5,312,910; 5,188,642; 4,940,835; 5,866,775; 6,225,114 B1; 6,130,366; 5,310,667; 4,535,060; 4,769,061; 5,633,448; 5,510,471; Re. 36,449; RE 37,287 E; and 5,491,288; and international publications EP1173580; WO 01/66704; EP1173581 and EP1173582, which are incorporated herein by ...
0137] B. Glyphosate (resistance conferred by mutant 5-enolpyruvlshikimate-3-phosphate synthase (EPSPS) and aroA genes, respectively) and other phosphono compounds, such as glufosinate (phosphinothricin acetyl transferase (PAT) and Streptomyces hygroscopicus PAT bar genes), pyridinoxy or phenoxy proprionic acids, and cyclohexanediones (ACCase inhibitor-encoding genes). See, for example, U.S. Pat. No. 4,940,835 to Shah, et al., which discloses the nucleotide sequence of a form of EPSPS which can confer glyphosate resistance. U.S. Pat. No. 5,627,061 to Barry, et al., also describes genes encoding EPSPS enzymes. See also, U.S. Pat. Nos. 6,566,587, 6,338,961, 6,248,876, 6,040,497, 5,804,425, 5,633,435, 5,145,783, 4,971,908, 5,312,910, 5,188,642, 4,940,835, 5,866,775, 6,225,114, 6,130,366, 5,310,667, 4,535,060, 4,769,061, 5,633,448, 5,510,471, RE 36,449, RE 37,287, and 5,491,288; and International Publications EP1173580, WO 01/66704, EP1173581, and EP1173582, which are incorporated herein by reference ...
Objective(s) Staphylococcus aureus is both a successful human commensal and a major pathogen. In this study we investigated the genetic diversity of 26 S. aureus isolates recovered from human skin and urinary tract infections. Materials and Methods Typing procedure for the studied S. aureus isolates was performed based on PCR amplification of the aroA gene, which encodes the enzyme 5-enolpyruvylshikmate-3-phosphate synthase (EPSPS) that involves in aromatic amino acid biosynthesis, and restriction fragment length polymorphism (RFLP) analysis of the product. Results All S. aureus isolates produced a single PCR amplification product of 1,153 bp. Digestion of the PCR products with the TaqI endonuclease revealed four different aroA gene patterns designated as A, B, N and H according to the nomenclature system of previous studies. In general, 80.77% of the studied isolates displayed type N, 7.69% were type B, 7.69% were type H and 3.85% displayed type A. Conclusion Divergent aroA types were detected
Scientists searching for clues to understand how superweeds obtain resistance to the popular herbicide glyphosate may have been missing a critical piece of information, a Purdue University study shows.
In 2008 and 2009, suspected glyphosate- resistant ryegrass samples were collected from a field in Franklin Parish (Figures 1 and 2). At the same time, ryegrass samples known to be susceptible to glyphosate were collected from a field at the Northeast Research Station in St. Joseph. Glyphosate at zero to 12 pounds per acre was applied to both populations when ryegrass was 6 to 8 inches tall with two to four tillers - shoots from the base of the plant. The amount of glyphosate required to reduce growth by 50 percent and dose responses were analyzed, and a seven-fold increase in glyphosate tolerance was observed in the Franklin Parish field compared with the Tensas Parish field. This is a strong indication of resistance. Every field in the state has not been tested for glyphosate resistance, but glyphosate resistance is likely in fields where this herbicide has failed to control ryegrass ...
Lawsuits are now beginning in the United States against Monsantos herbicide Roundup, the number one herbicide in the world used in modern agriculture. The
A 2016 study carried out by the Heinrich Böll Foundation in Germany has found that 99.6% of the German population has been contaminated by the herbicide
Fmoc-4-(phosphonomethyl)-phenylalanine 229180-64-7 safety info, Fmoc-4-(phosphonomethyl)-phenylalanine chemical safety search, Chemical Fmoc-4-(phosphonomethyl)-phenylalanine safety technical specifications ect.
Glyphosate, a chemical that controls weeds and grasses, is the most widely used herbicide worldwide. The major reason glyphosate is used so broadly is because many crops have been genetically engineered to be resistant to glyphosate, allowing it to target weeds while leaving crops unaffected. When applied as a mixture with other substances, plants can more readily absorb glyphosate, making it more effective. People can be exposed to residual amounts of glyphosate by ingestion of food or water; however, individuals who regularly handle glyphosate products as part of their occupation experience higher exposures ...
The profuse use of glyphosate on our crops and environment is a public health emergency. If you do not know the details of the herbicide glyphosate, please pay attention - you are eating, drinking and touching it all day, everyday. I recount the groundbreaking trials of cancer victims vs. Monsanto, so that we can all better understand that RoundUp is genotoxic and a known carcinogen. And thats just the beginning.
The NK603 line of maize was developed to allow the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option. NK603 contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows the plant to survive the otherwise lethal application of glyphosate ...
March 15, 2017 by: Mike Adams. (Natural News) Court documents released on March 13th show that Monsanto colluded with the EPA to bury scientific evidence linking its glyphosate product (RoundUp) to cancer in humans (specifically, Non-Hodgkins Lymphoma).. Glyphosate.news has published the full text of the court document Motion to Compel Deposition of Jess Rowland.. Also published on Glyphosate.news is this heartbreaking letter from a dying EPA scientist begging her colleagues to stop lying about the dangers of glyphosate.. Continuing with the full disclosure of these court documents - originally acquired by U.S. Right to Know - Glyphosate.news has now published the full text of the Third-Party Discovery and Pending Motions to Seal document.. Some of the more interesting highlights from this document include these passages:. Monsanto has trumpeted reports generated by the Environmental Protection Agency (EPA) concluding that glyphosate is not hazardous… In this phase, which is limited to ...
A tall waterhemp population from Missisippi was suspected to be resistant to glyphosate. Glyphosate dose response experiments resulted in GR50 (dose required to reduce plant growth by 50%) values of 1.28 and 0.28 kg ae ha-1 glyphosate for the glyphosate-resistant (GR) and -susceptible (GS) populations, respectively, indicating a five-fold resistance. The absorption pattern of 14C-glyphosate between the GR and GS populations was similar up to 24 h after treatment (HAT). Thereafter, the susceptible population absorbed more glyphosate (55 and 49% of applied) compared to the resistant population (41 and 40% of applied) by 48 and 72 HAT, respectively. Treatment of a single leaf in individual plants with glyphosate at 0.84 kg ha-1, in the form of 10 1-µl droplets, provided greater control (85 vs. 29%) and shoot fresh weight reduction (73 vs. 34% of nontreated control) of the GS plants compared to the GR plants, possibly indicating a reduced movement of glyphosate in the GR plants. The amount of ...
A tall waterhemp population from Missisippi was suspected to be resistant to glyphosate. Glyphosate dose response experiments resulted in GR50 (dose required to reduce plant growth by 50%) values of 1.28 and 0.28 kg ae ha-1 glyphosate for the glyphosate-resistant (GR) and -susceptible (GS) populations, respectively, indicating a five-fold resistance. The absorption pattern of 14C-glyphosate between the GR and GS populations was similar up to 24 h after treatment (HAT). Thereafter, the susceptible population absorbed more glyphosate (55 and 49% of applied) compared to the resistant population (41 and 40% of applied) by 48 and 72 HAT, respectively. Treatment of a single leaf in individual plants with glyphosate at 0.84 kg ha-1, in the form of 10 1-µl droplets, provided greater control (85 vs. 29%) and shoot fresh weight reduction (73 vs. 34% of nontreated control) of the GS plants compared to the GR plants, possibly indicating a reduced movement of glyphosate in the GR plants. The amount of ...
Famously corrupt and unscrupulous, Monsanto Corporation has now been discovered in covering up the highly toxic effects of the secret additives it combines with glyphosate in Roundup, the worlds most-used herbicide. The IARC, an agency of the World Health Organization, released a report in March, 2015 that declared the chemical glyphosate to be probably carcinogenic to humans (Group 2A). They were not provided tests that included the effects of glyphosate combined with specific trade secret additives. Monsanto is desperate to hide the true carcinogen in its Roundup weed-killer.. Glyphosate is the largest component of Monsanto Roundup, the worlds largest weed-killer and the toxin mandated in every Monsanto Genetically Manipulated (GMO) planting. But what Monsanto refuses to disclose is what additives it uses, otherwise termed surfactants or adjuvants, ostensibly to give the glyphosate a turbo weed-killer effectiveness boost.. Since late 2016 the United States District Court for the Northern ...
Unfortunately, the EPA has had a wishy-washy approach to regulating the use of glyphosate with regards to its reported adverse effects on human health. In its report, the agency said glyphosate is not likely carcinogenic to humans.. However, the International Agency for Research on Cancer (IARC) determined that glyphosate was a probable human carcinogen in 2015. The EPA had even agreed with this classification before it backtracked. Last October, it approved the use of Bayer AGs glyphosate-based weedkiller Roundup for the next five years.. Given the EPAs inconsistencies regarding its verdict on glyphosate, the advocacy group Center for Food Safety (CFS) filed a lawsuit against the agency last March in the Ninth Circuit Court of Appeals. Representing a broad coalition of farmers and conservationists, the CFS seeks to have glyphosate prohibited from use or sale to protect users from its adverse effects.. Last December, the CFS filed its opening arguments and evidence in its litigation ...
Glyphosate has been found in malformed piglets. The research study was conducted by a team of researchers from Germany and Egypt in collaboration with the Danish pig farmer Ib Pedersen, whose pigs were analysed for glyphosate content.The rate of malformations increased to one out of 260 born piglets if sow feeds contained 0.87-1.13 ppm glyphosate in the first 40 days of pregnancy. In the case of 0.25 ppm glyphosate in sow feeds, one out of 1432 piglets was malformed. In this case, therefore, a higher dose of glyphosate led to more malformations.. The piglets showed different abnormalities, including ear atrophy, spinal and cranial deformations, hole in the skull, and leg atrophy. In one piglet, one eye was not developed; it had a single large one (cyclopia, a malformation observed in Argentine populations exposed to Roundup spraying). There were piglets without a trunk, with an elephant tongue, and a female piglet with testes. One malformed piglet had a swollen belly and the foregut and ...
Xtend soybeans were planted on 1 million acres in 2016, and by 2020 were projected to be planted on 50 million acres. Some ... 3] Caviness, Charles E.; Walters, H.J. (1971). "Effect of phytophthora rot on yield and chemical composition of soybean seed". ... 1] National Agriculture Statistics Service (2005) in Acreage eds. Johanns, M. & Wiyatt, S. D. 6 30, (U.S. Dept. of Agriculture ... 1-carboxyvinyl)-transferase) that is resistant to glyphosate inhibition. The version used in genetically modified crops was ...
2 (1): 68-77. doi:10.1208/ps020108. PMC 2751003. PMID 11741224. Loo JA, DeJohn DE, Du P, Stevenson TI, Ogorzalek Loo RR (July ... As such a term similar to the one proposed above to modulate Vmax should be appropriate in most situations: K m 1 − ( K m 1 − K ... 36 (1): 13-24. doi:10.1016/S0041-0101(97)00074-3. PMID 9604278. Holmes CF, Maynes JT, Perreault KR, Dawson JF, James MN ( ... 1701 (1-2): 1-14. doi:10.1016/j.bbapap.2004.06.004. PMID 15450171. Web tutorial on enzyme inhibition, Tutorial by Dr Peter ...
... endo-1,4-beta xylanases MeSH D08.811.277.450.950.500 - xylan endo-1,3-beta-xylosidase MeSH D08.811.277.656.149 - atp-dependent ... glycogen synthase kinase 3 MeSH D08.811.913.696.620.682.700.494 - i-kappa B kinase MeSH D08.811.913.696.620.682.700.559 - map ... glucan 1,4-beta-glucosidase MeSH D08.811.277.450.420.200.600 - glucan endo-1,3-beta-d-glucosidase MeSH D08.811.277.450.420.375 ... map kinase kinase 1 MeSH D08.811.913.696.620.682.700.565.200 - map kinase kinase 2 MeSH D08.811.913.696.620.682.700.565.300 - ...
72 (1): 53-62. doi:10.1016/0168-9452(90)90186-r. Abdel-Meguid SS, Smith WW, Bild GS (Dec 1985). "Crystallization of 5- ... 87 (1): 232-8. doi:10.1104/pp.87.1.232. PMC 1054731. PMID 16666109. Pollegioni L, Schonbrunn E, Siehl D (Aug 2011). "Molecular ... 186 (3): 673. doi:10.1016/0022-2836(85)90140-8. PMID 3912512. Ream JE, Steinrücken HC, Porter CA, Sikorski JA (May 1988). " ... 63 (1): 73-105. doi:10.1146/annurev-arplant-042811-105439. PMID 22554242. The AAA pathways consist of the shikimate pathway ( ...
1. 1. −. [. I. ]. [. I. ]. +. K. i. =. V. max. −. V. max. [. I. ]. [. I. ]. +. K. i. {\displaystyle {\cfrac {V_{\max }}{\cfrac ... 1. +. [. I. ]. K. i. =. V. max. [. I. ]. +. K. i. K. i. {\displaystyle {\cfrac {V_{\max }}{1+{\cfrac {\ce {[I]}}{K_{i}}}}}={\ ... 1. −. (. K. m. 1. −. K. m. 2. ). [. X. ]. [. X. ]. +. K. x. {\displaystyle K_{m1}-(K_{m1}-K_{m2}){\cfrac {\ce {[X]}}{[{\ce {X ... 1. −. (. V. max. 1. −. V. max. 2. ). [. I. ]. [. I. ]. +. K. i. {\displaystyle V_{\max 1}-(V_{\max 1}-V_{\max 2}){\cfrac {\ce ...
3-deoxy-D-manno-octulosonic acid kinase EC 2.7.1.167: D-glycero-beta-D-manno-heptose-7-phosphate kinase EC 2.7.1.168: D-glycero ... D-inositol-3-phosphate glycosyltransferase EC 2.4.1.251: GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1- ... inositol 3-a-galactosyltransferase EC 2.4.1.124: now included with EC 2.4.1.87 EC 2.4.1.125: sucrose-1,6-a-glucan 3(6)-a- ... 4-benzoxazin-3(4H)-one 2 -D-glucosyltransferase EC 2.4.1.203: trans-zeatin O-b-D-glucosyltransferase EC 2.4.1.204: now EC 2.4. ...
... to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate. ... Ref.1. "Cloning and characterization of the aroA gene from Mycobacterium tuberculosis.". Garbe T., Jones C., Charles I.G., ... Ref.1. "Cloning and characterization of the aroA gene from Mycobacterium tuberculosis.". Garbe T., Jones C., Charles I.G., ... Ref.1. "Cloning and characterization of the aroA gene from Mycobacterium tuberculosis.". Garbe T., Jones C., Charles I.G., ...
1.70 Angstrom resolution structure of 3-phosphoshikimate 1-carboxyvinyltransferase(AroA) from Coxiella burnetii in complex with ... 3-phosphoshikimate 1-carboxyvinyltransferase A, B, C, D 441 Coxiella burnetii EC#: 2.5.1.19 IUBMB Gene Name(s): aroA CBU_0526 ... 3R,4S,5R)-3,4,5-TRIHYDROXYCYCLOHEX-1-ENE- 1-CARBOXYLIC ACID. SHIKIMATE (Synonym). C7 H10 O5 JXOHGGNKMLTUBP-HSUXUTPPSA-N ... 1,2-ETHANEDIOL. ETHYLENE GLYCOL (Synonym). C2 H6 O2 LYCAIKOWRPUZTN-UHFFFAOYSA-N ...
... to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate. ... TIGR01356, aroA, 1 hit. PROSITEi. View protein in PROSITE. PS00104, EPSP_SYNTHASE_1, 1 hit. PS00885, EPSP_SYNTHASE_2, 1 ... Q9JTT3-1 [UniParc]FASTAAdd to basketAdded to basket. « Hide. 10 20 30 40 50. MTESLRLPAA SLKPSTVALP GSKSISNRTL LLAALSDNAC ... Last modified:October 1, 2000 - v1. ,p>The checksum is a form of redundancy check that is calculated from the sequence. It is ...
3-phosphoshikimate 1-carboxyvinyltransferase (aroA) Recombinant Protein-WP_004685324.1 (MBS1047607) product datasheet at ... 0.05 mg (E-Coli) / $870 +1 FREE 8GB USB. 0.05 mg (Yeast) / $940 +1 FREE 8GB USB. 0.2 mg (E-Coli) / $1,160 +1 FREE 8GB USB. 0.05 ... 1 mg (E-Coli) / $1,940 +2 FREE 8GB USB. 0.5 mg (Baculovirus) / $2,200 +2 FREE 8GB USB. 0.1 mg (Mammalian-Cell) / $2,285 +2 FREE ... 0.2 mg (Yeast) / $1,275 +1 FREE 8GB USB. 0.5 mg (E-Coli) / $1,280 +1 FREE 8GB USB. 0.05 mg (Mammalian-Cell) / $1,410 +1 FREE ...
3-phosphoshikimate 1-carboxyvinyltransferase (aroA) Recombinant Protein-WP_000245895.1 (MBS1381469) product datasheet at ... 1 mg (E-Coli) / $1,895 +2 FREE 8GB USB. 0.5 mg (Baculovirus) / $2,170 +2 FREE 8GB USB. 1 mg (Yeast) / $2,255 +2 FREE 8GB USB. ... 0.05 mg (E-Coli) / $850 +1 FREE 8GB USB. 0.05 mg (Yeast) / $925 +1 FREE 8GB USB. 0.2 mg (E-Coli) / $1,135 +1 FREE 8GB USB. 0.05 ... 0.5 mg (E-Coli) / $1,250 +1 FREE 8GB USB. 0.2 mg (Yeast) / $1,255 +1 FREE 8GB USB. 0.05 mg (Mammalian-Cell) / $1,395 +1 FREE ...
Purchase Recombinant Aeromonas salmonicida 3-phosphoshikimate 1-carboxyvinyltransferase(aroA). It is produced in Yeast. High ... 1-427. Target Protein Sequence. MNSLRLEPIS RVAGEVNLPG SKSVSNRALL LAALARGTTR LTNLLDSDDI RHMLAALTQL GVKYKLSADK TECTVHGLGR ... Recombinant Aeromonas salmonicida 3-phosphoshikimate 1-carboxyvinyltransferase(aroA). Recombinant Aeromonas salmonicida 3- ... Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) ...
... entry for 2o0b.1. Mycobacterium tuberculosis epsp synthase in complex with S3P (partially photolyzed) ... 3 x SO4: SULFATE ION(Non-functional Binders). SO4.2: 10 residues within 4Å:*. Chain A: K.23, L.94, A.95, G.96, T.97, R.124, Q. ... 1 x S3P: SHIKIMATE-3-PHOSPHATE(Non-covalent). S3P.5: 15 residues within 4Å:*. Chain A: K.23, S.24, R.28, T.97, F.101, S.167, S. ... 1 x PO4: PHOSPHATE ION(Non-functional Binders). PO4.1: 8 residues within 4Å:*. Chain A: A.166, S.167, S.168, P.195, S.196, H. ...
3-phosphoshikimate 1-carboxyvinyltransferase. Magnetococcus marinus (strain ATCC BAA-1437 / JCM 17883 / MC-1). Loading... ... 3-phosphoshikimate 1-carboxyvinyltransferase. Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334). ... 3-phosphoshikimate 1-carboxyvinyltransferase. Methanothrix thermoacetophila (strain DSM 6194 / JCM 14653 / NBRC 101360 / PT). ... 3-phosphoshikimate 1-carboxyvinyltransferase. Pelobacter propionicus (strain DSM 2379 / NBRC 103807 / OttBd1). Loading... ...
... and 5-enolpyruvylshikimate-3-phosphate synthase (AroA) derived from X-ray structures of their tetrahedral reaction intermediate ... 3-phosphoshikimate 1-carboxyvinyltransferase Chain: A Molecule details › Chain: A. Length: 427 amino acids. Theoretical weight: ... Assembly 1 (atom only; mmCIF). FASTA (Entry). SIFTS XML file with residue-level mappings. Summary report (PDF). Full report ( ... P0A6D3 (Residues: 1-427; Coverage: 100%). Gene names: JW0891, aroA, b0908. Sequence domains: EPSP synthase (3-phosphoshikimate ...
3-phosphoshikimate 1-carboxyvinyltransferase. Escherichia coli (strain K12 / MC4100 / BW2952) ... 2009C-4446: A0A028E715 ; Escherichia coli 3-373-03_S4_C2: A0A069XQS1 ; Escherichia coli 2-210-07_S3_C3: A0A070SRU3 ; ... 3-phosphoshikimate 1-carboxyvinyltransferase UniProtKBInterProInteractive Modelling. 427 aa; Sequence (Fasta) 85 identical ... STRUCTURE OF EPSP SYNTHASE LIGANDED WITH SHIKIMATE-3-PHOSPHATE AND GLYPHOSATE. monomer 10×FMT; 1×S3P; 1×GPJ;. 1g6s. 1-427. ...
1.. Open image in new window. Fig. 1 Capsular typing of the strains. Explanation: Figure showing positive controls working on ... 1.. Biberstein EL. The pasteurelloses. In: Handbook series in zoonoses, H. Stoenner, M. Torten and W. Kaplan (eds.). CRC Press ... 1.Research Institute for Biological Safety ProblemsMinistry of Education and Science of Republic of Kazakhstan - Science ... 3.Research Institute of Wildlife EcologyUniversity of Veterinary Medicine ViennaViennaAustria ...
5-Enolpyruvylshikimate 3-phosphate (EPSP) synthase (3-phosphoshikimate 1-carboxyvinyltransferase; EC 2.5.1.19), 3- ... 3-Dehydroquinate synthase (EC 4.6.1.3) and shikimate kinase (EC 2.7.1.71) were detected together with the other pathway enzymes ... Lambert, J.M., Boocock, M.R., Coggins, J.R, (1985) The 3-dehydroquinate synthase of the arom enzyme complex of Neurospora ... Bowen, J.R., Kosuge, T. (1977) The formation of shikimate-3-phosphate in cell-free preparations of Sorghum. Phytochemistry 16, ...
EC 2.5.1. See separate file for EC 2.5.1.51 to EC 2.5.1.100 and EC 2.5.1.101 to EC 2.5.1.151 Contents. EC 2.5.1.1 dimethylallyl ... Int. 3 (1981) 669-675.. [EC 2.5.1.29 created 1984, modified 2011]. EC 2.5.1.30. Accepted name: heptaprenyl diphosphate synthase ... Glossary: O-acetyl-L-serine = (2S)-3-acetyloxy-2-aminopropanoic acid. Other name(s): O-acetyl-L-serine sulfhydrylase; O-acetyl- ... putrescine = butane-1,4-diamine. S-adenosyl 3-(methylsulfanyl)propylamine = (3-aminopropyl){[(2S,3S,4R,5R)-5-(6-amino-9H-purin- ...
... carboxyvinyltransferase is the sixth enzyme in the shikimate pathway and catalyzes the conversion of shikimate-3-phosphate and ... P 1 21 1 Unit Cell. a=46.25Å, b=186.64Å, c=95.79Å. α=90.00, β=91.51, γ=90.00 Solvent content. Matthews coefficient. Refinement ... This protein was co-crystallized with the substrate shikimate-3-phosphate and the commercially successful herbicide glyphosate ... Glyphosate binds adjacent to shikimate-3-phosphate in phosphoenolpyruvates binding site. Interestingly, two C-terminal apo ...
... associated receptor kinase 1, pecmet is pectin methylesterase, serthre is receptor serine-threonine protein, vichyd is vicianin ... associated receptor kinase 1, pecmet is pectin methylesterase, serthre is receptor serine-threonine protein, vichyd is vicianin ... and a brassinosteroid insensitive 1-associated receptor kinase (Table 4). There was no evidence that the scaffold identified as ... and a brassinosteroid insensitive 1-associated receptor kinase (Table 4). There was no evidence that the scaffold identified as ...
Column D: Organism (Only 1) (required) *Please list only one organism where the enzyme listed in Column A has been ...
1.↵. Alexander, J. E., P. W. Andrew, D. Jones, and I. S. Roberts. 1993. Characterization of an aromatic amino acid-dependent ... The levels of significance by the Mann-Whitney test were as follows: P = 0.04 at 1 h (A) and P = 0.004 at day 1 and P = 0.03 at ... 1. (A) Schematic representation of the aro operon and surrounding genes according to the published sequences of S. aureus COL ( ... 3.↵. Bowe, F., P. OGaora, D. Maskell, M. Cafferkey, and G. Dougan. 1989. Virulence, persistence, and immunogenicity of ...
125000000753 cycloalkyl group Chemical group 0 claims description 3 * 150000001204 N-oxides Chemical class 0 claims description ... 125000004648 C2-C8 alkenyl group Chemical group 0 description 3 * 125000004649 C2-C8 alkynyl group Chemical group 0 description ... 241001310178 Watermelon mosaic virus Species 0 description 3 * 241000723854 Zucchini yellow mosaic virus Species 0 description ... 229910052943 magnesium sulfate Inorganic materials 0 description 3 * 239000004127 magnesium sulphate Substances 0 description 3 ...
108010061844 Poly(ADP-ribose) Polymerases Proteins 0 description 3 * 102000012338 Poly(ADP-ribose) Polymerases Human genes 0 ... 239000005667 attractants Substances 0 claims description 3 * 230000031902 chemoattractant activity Effects 0 claims description ... 102000005370 poly(ADP-ribose) glycohydrolase family Human genes 0 description 3 * 159000000001 potassium salts Chemical class 0 ... 235000006618 Brassica rapa subsp oleifera Nutrition 0 description 3 * 235000004977 Brassica sinapistrum Nutrition 0 description ...
2005, 3 (2): e38-10.1371/journal.pbio.0030038.PubMedPubMed CentralView ArticleGoogle Scholar. ... 2004, 134 (1): 67-80. 10.1104/pp.103.030536.PubMedPubMed CentralView ArticleGoogle Scholar. ... 2005, 138 (3): 1216-1231. 10.1104/pp.105.060988.PubMedPubMed CentralView ArticleGoogle Scholar. ... 2007, 17 (1): 108-116. 10.1101/gr.5488207.PubMedPubMed CentralView ArticleGoogle Scholar. ...
FIGURE 1. Figure 1. The shikimate and phenylalanine derived secondary metabolite biosynthesis in plants. (A) Shikimate ... FIGURE 3. Figure 3. Heat map for isoforms of shikimate-phenylalanine biosynthetic genes in plant genomes and hypothetical ... 3-Deoxy-D-Arabino-Heptulosonate 7-Phosphate Synthase. The first enzymatic step of the shikimate pathway, 3-deoxy-D-arabino- ... 3-Dehydroquinate Synthase. The second step of the shikimate pathway is catalyzed by 3-dehydroquinate synthase (DHQS), an enzyme ...
... carboxyvinyltransferase from Vibrio cholerae in complex with shikimate-3-phosphate (partially photolyzed) and glyphosate. ... Title: Crystal structure of aminoglycoside acetyltransferase AAC(3)-Ib. Authors: P.J.Stogios,Z.Xu,E.Evdokimova,V.Yim,W.F. ... Title: 2.75 Angstrom Crystal Structure of Enolase 1 from Toxoplasma gondii.. Authors: G.Minasov,J.Ruan,L.Shuvalova,A.Halavaty,H ... Title: X-Ray Crystal Structure of a Fragment (1-75) of a Transcriptional Regulator PdhR from Escherichia coli CFT073 ...
Chemical reaction (3) KEGG ENZYME (3) Literature (1) PubMed (1) Taxonomy (1) TAX (1) All databases (5) Download RDF ... REFERENCE 1 (bases 1 to 2108637) AUTHORS Tae,H., Shallom,S., Settlage,R., Hawkins,G.N., Adams,L.G. and Garner,H.R. TITLE ... 194 (4), 910 (2012) PUBMED 22275106 REFERENCE 2 (bases 1 to 2108637) AUTHORS Tae,H., Shallom,S., Settlage,R., Hawkins,G.N., ... FEATURES Location/Qualifiers source 1..2108637 /organism="Brucella suis VBI22" /mol_type="genomic DNA" /strain="VBI22" /db_xref ...
2005;1:e27.View ArticlePubMed CentralPubMedGoogle Scholar. *. Price DRG, Feng H, Baker JD, Bavan S, Luetje CW, Wilson ACC. ... 2011;56:1-19.View ArticlePubMedGoogle Scholar. *. Hu J, De Barro P, Zhao H, Wang J, Nardi F, Liu SS. An extensive field survey ... 2002;18 Suppl 1:S225-232.View ArticlePubMedGoogle Scholar. *. Cottret L, Wildridge D, Vinson F, Barrett MP, Charles H, Sagot MF ... 2006;314:312-3.View ArticlePubMedGoogle Scholar. *. Sloan DB, Moran NA. Genome reduction and co-evolution between the primary ...
TABLE 1. Table 1. Statistics of the four metagenomic data sets derived after quality trimming from samples of a water kefir ... FIGURE 3. Figure 3. Metagenomic recruitment plots of species or genera found in the water kefir fermentation samples. Each dot ... TABLE 3. Table 3. Statistics of the assembly of the metagenomic sequence reads into contigs of four water kefir fermentation ... 2017). 4,3-α-glucanotransferase, a novel reaction specificity in glycoside hydrolase family 70 and clan GH-H. Sci. Rep. 7:39761 ...
Shwe, EiEi; Huang, Shuang-Quan; Show all 3 Authors. Source:. Plant diversity 2020 v.42 no.3 pp. 142-147. ISSN:. 2468-2659. ... Climate dynamics 2020 v.54 no.3-4 pp. 2387-2402. ISSN:. 0930-7575. Subject:. El Nino; General Circulation Models; advection; ... 1. An integrated photogrammetric and photoclinometric approach for illumination-invariant pixel-resolution 3D mapping of the ... Food & function 2020 v.11 no.3 pp. 2427-2438. ISSN:. 2042-650X. Subject:. Alpinia; Alzheimer disease; Oriental traditional ...
Buy 3 items of this product for 198.00 €/items Save 32.2Item % ... EPYC1 , Essential Pyrenoid Component 1. 292 € Info. Add to cart ... Buy 3 items of this product for 198.00 €/items Save 32.2Item % ... Buy 3 items of this product for 198.00 €/items Save 32.2Item % ... Buy 3 items of this product for 198.00 €/items Save 32.2Item % ... Buy 3 items of this product for 211.00 €/items Save 30.1Item % ... Buy 3 items of this product for 198.00 €/items Save 32.2Item % ... Buy 3 items of this product for 198.00 €/items Save 32.2Item % ...
10.1007/s00425-011-1368-1.PubMedView ArticleGoogle Scholar. *. Devaiah BN, Karthikeyan AS, Raghothama KG: WRKY75 transcription ... NO3)2.4H2O, 1.25 mM NH4NO3, 0.1 mM KCl, 0.65 mM K2SO4, 0.65 mM MgSO4, 10.0 mM H3BO3, 0.5 mM (NH4)6Mo7O24, 1.0 mM MnSO4, 0.1 mM ... As shown in Table 1 and Figure 1, after 3 days of LP treatment, the Pi contents in both roots and shoots had decreased slightly ... After 2-3 days of re-culturing in SP nutrient solutions, half of the seedlings were transplanted into a low-phosphate (LP, same ...
Xtend soybeans were planted on 1 million acres in 2016, and by 2020 were projected to be planted on 50 million acres. Some ... 3] Caviness, Charles E.; Walters, H.J. (1971). "Effect of phytophthora rot on yield and chemical composition of soybean seed". ... 1] National Agriculture Statistics Service (2005) in Acreage eds. Johanns, M. & Wiyatt, S. D. 6 30, (U.S. Dept. of Agriculture ... 1-carboxyvinyl)-transferase) that is resistant to glyphosate inhibition. The version used in genetically modified crops was ...
The K(m) for holoBCCP87 was 0.45 +/- 0.07 mM while the V(max) was 2031.8 +/- 231.0 min(-1). The V/K or catalytic efficiency of ... Crystals belong to space group P2(1)2(1)2(1) with the following unit cell dimensions: a = 58. 4 A, b = 87.8 A, and c = 101.6 A ... The BC subunit of PC from Aquifex aeolicus (PC-beta) was crystallized in an orthorhombic form with space group P2(1)2(1)2, unit ... The crystals are grown by microdialysis against 10 mM potassium phosphate (pH 7.0), 1 mM EDTA, 2 mM DTT and 1 mM NaN3 at 4 ...
  • protein of unknown function tnfn1_pw060420p02q181 FTN_0933 protein of unknown function tnfn1_pw060323p04q134 FTN_0938 hypothetical protein tnfn1_pw060419p01q170 FTN_1006 transporter-associated protein, HlyC/CorC family tnfn1_pw060323p08q141 FTN_1015 isochorismatase family protein tnfn1_pw060418p01q153 FTN_1055 lon DNA-binding, ATP-dependent protease La tnfn1_pw060510p01q114 FTN_1073 DNA/RNA endonuclease G tnfn1_pw060419p04q168 FTN_1091 aroA 3-phosphoshikimate 1- carboxyvinyltransferase tnfn1_pw060328p08q188 FTN_1098 conserved. (lbl.gov)
  • The initial enzyme of the shikimate pathway, 3-deoxy- d -arabino-heptulosonate 7-phosphate synthase (EC 4.1.2.15) was also associated with intact density-gradient-purified chloroplasts. (springer.com)
  • 3-Dehydroquinate synthase (EC 4.6.1.3) and shikimate kinase (EC 2.7.1.71) were detected together with the other pathway enzymes in stromal preparations from washed chloroplasts. (springer.com)
  • Top blast descriptors are as follows: ceram is ceramidase family protein, brass is brassinosteroid insensitive 1−associated receptor kinase 1, pecmet is pectin methylesterase, serthre is receptor serine-threonine protein, vichyd is vicianin hydrolase-live, p450_0 is cytochrome p450 82a3-like, germd is (-)-germacrene d synthase, and helica is u5 small nuclear ribonucleoprotein helicase. (nih.gov)
  • Furthermore, one of the enzymes of the pathway - 5- enol pyruvalshikimate-3-phosphate synthase (EPSP) - is one of the most widely employed herbicide target sites (see, Duke and Powles, 2008 ). (frontiersin.org)
  • How the mutation glycine96 to alanine confers glyphosate insensitivity to 5-enolpyruvyl shikimate-3-phosphate synthase from Escherichia coli. (nih.gov)
  • The enzyme 5-enolpyruvyl shikimate-3-phosphate (EPSP) synthase (EC 2.5.1.19) is essential for the biosynthesis of aromatic compounds in plants and microbes and is the unique target of the herbicide glyphosate. (nih.gov)
  • IDP00009 3-dehydroquinate synthase BA_1538 [Bacillus anthracis str. (sanfordburnham.org)
  • IDP00012 3-phosphoshikimate 1-carboxyvinyltransferase (5-enolpyruvylshikimate-3-phosphate synthase) (EPSP synthase) (EPSPS) BA_2953 [Bacillus anthracis str. (sanfordburnham.org)
  • We are studying two enzymes from the shikimate pathway, dehydroquinate synthase (DHQS) and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). (ox.ac.uk)
  • Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate. (uniprot.org)
  • phosphoenolpyruvate:3-phosphoshikimate 5-O-(1-carboxyvinyl)-transferase) that is resistant to glyphosate inhibition. (wikipedia.org)
  • 3-phosphoshikimate 1- carboxyvinyltransferase is the sixth enzyme in the shikimate pathway and catalyzes the conversion of shikimate-3-phosphate and phosphoenolpyruvate to 5-enolpyruvyl-3-shikimate phosphate. (csgid.org)
  • The mutant enzyme is insensitive to glyphosate with unaltered affinity for its first substrate, shikimate-3-phosphate (S3P), but displays a 30-fold lower affinity for its second substrate, phosphoenolpyruvate (PEP). (nih.gov)
  • 324050 FT /organism="Tropheryma whipplei TW08/27" FT /strain="TW08/27" FT /mol_type="genomic DNA" FT /db_xref="taxon:218496" FT CDS _ pept 1. (univ-lyon1.fr)
  • EC 2.5.1.19), 3-dehydroquinate dehydratase (EC 4.2.1.10) and shikimate: NADP + oxidoreductase (EC 1.1.1.25) were present in intact chloroplasts and root plastids isolated from pea seedling extracts by sucrose and modified-silica density gradient centrifugation. (springer.com)
  • IDP00015 UDP-N-acetylenolpyruvoylglucosamine reductase 1 (UDP-N-acetylmuramate dehydrogenase 1) BA_4048 [Bacillus anthracis str. (sanfordburnham.org)
  • 3-phosphoshikimate 1-carboxyvinyltransferase [Aeromonas hydrophila subsp. (violinet.org)
  • The mutant exhibited a 50% lethal dose (1 × 10 6 CFU/mouse) higher than that of the parental strain (4.3 × 10 4 CFU/mouse). (asm.org)
  • Flavihumibacter solisilvae 3-3 T (= KACC 17917 T = JCM 19891 T ) represents a type strain of the genus Flavihumibacter within the family Chitinophagaceae . (biomedcentral.com)
  • The genome analysis of F. solisilvae 3-3 T will provide the genomic basis for better understanding these mechanisms and applying the strain to industries and bioremediation more efficiently. (biomedcentral.com)
  • One of the isolated mutants, designated strain EC-2, produced 90% higher electric current than wild-type MR-1 in ECs and was found to have a transposon inserted in the SO_1860 ( uvrY ) gene, which encodes a DNA-binding response regulator of the BarA/UvrY two-component regulatory system. (beds.ac.uk)
  • Shewanella oneidensis MR-1 exhibits extracellular electron transfer (EET) activity that is influenced by various cellular components, including outer-membrane cytochromes, cell-surface polysaccharides (CPS), and regulatory proteins. (beds.ac.uk)
  • Aromatic-dependent mutants of Salmonella enterica serovar Typhimurium ( 38 ), Yersinia pestis ( 40 ), Bordetella pertussis ( 50 ), Corynebacterium pseudotuberculosis ( 53 ), Pseudomonas aeruginosa ( 44 ), and Listeria monocytogenes ( 1 ) have been shown to be avirulent and to stimulate protective immunity in different hosts. (asm.org)
  • and (ii) a polypeptide having 3-dehydroshikimate dehydratase (3DSD) activity. (justia.com)
  • The crystal structure of 3-hydroxydecanoyl-(acyl carrier protein) dehydratase from Vibrio cholerae O1 biovar El Tor str. (csgid.org)
  • Bowen, J.R., Kosuge, T. (1977) The formation of shikimate-3-phosphate in cell-free preparations of Sorghum . (springer.com)
  • This protein was co-crystallized with the substrate shikimate-3-phosphate and the commercially successful herbicide glyphosate. (csgid.org)
  • Glyphosate binds adjacent to shikimate-3-phosphate in phosphoenolpyruvate's binding site. (csgid.org)
  • 1 The other plausible reason for the intervention of computational approaches is to avoid unnecessary risks from research experiments related to bio-war pathogens like Anthrax , Clostridium , and Brucella , and at the same time provide prospective research feasibilities to unveil drug targets in harmful pathogens. (dovepress.com)
  • Despite these studies, a deeper understanding of the molecular mechanisms of EET is required to optimize and enhance microbial electron transfer rates in BESs because studies in S. oneidensis MR-1 have also indicated that EET is a complex process that is influenced by various intracellular and extracellular components. (beds.ac.uk)
  • Seven of the 12 transcripts that were down-regulated in resistant compared to susceptible families were annotated as a viacianin hydrolase-like protein, a pectin methylesterase, a ceramidase family protein, an ATP-binding protein, and a brassinosteroid insensitive 1-associated receptor kinase (Table 4). (nih.gov)
  • F. solisilvae 3-3 T owns a genome size of 5.41 Mbp, 47 % GC content and a total of 4,698 genes, including 4,215 protein coding genes, 439 pseudo genes and 44 RNA encoding genes. (biomedcentral.com)
  • In both EC-2 and ∆SO_1860, the transcription of genes related to CPS synthesis was decreased compared to wild-type MR-1, suggesting that CPS negatively affects current generation. (beds.ac.uk)
  • In this study, the draft genomic information of F. solisilvae 3-3 T is described. (biomedcentral.com)
  • Interactions between endosymbionts and insects are widespread in nature [ 1 , 2 ], and are particularly important in members of the suborder Sternorrhyncha such as whiteflies, aphids, psyllids and mealybugs. (biomedcentral.com)
  • Xtend soybeans were planted on 1 million acres in 2016, and by 2020 were projected to be planted on 50 million acres. (wikipedia.org)
  • Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate. (uniprot.org)