3-Phosphoshikimate 1-Carboxyvinyltransferase: An enzyme of the shikimate pathway of AROMATIC AMINO ACID biosynthesis, it generates 5-enolpyruvylshikimate 3-phosphate and ORTHOPHOSPHATE from PHOSPHOENOLPYRUVATE and shikimate-3-phosphate. The shikimate pathway is present in BACTERIA and PLANTS but not in MAMMALS.Coxiella burnetii: A species of gram-negative bacteria that grows preferentially in the vacuoles of the host cell. It is the etiological agent of Q FEVER.Coxiella: A genus of gram-negative, rod-shaped bacteria that is widely distributed in TICKS and various mammals throughout the world. Infection with this genus is particularly prevalent in CATTLE; SHEEP; and GOATS.Alkyl and Aryl Transferases: A somewhat heterogeneous class of enzymes that catalyze the transfer of alkyl or related groups (excluding methyl groups). EC 2.5.Shikimic Acid: A tri-hydroxy cyclohexene carboxylic acid important in biosynthesis of so many compounds that the shikimate pathway is named after it.Q Fever: An acute infectious disease caused by COXIELLA BURNETII. It is characterized by a sudden onset of FEVER; HEADACHE; malaise; and weakness. In humans, it is commonly contracted by inhalation of infected dusts derived from infected domestic animals (ANIMALS, DOMESTIC).Crystallography, X-Ray: The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Crystallization: The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Genomics: The systematic study of the complete DNA sequences (GENOME) of organisms.Structural Homology, Protein: The degree of 3-dimensional shape similarity between proteins. It can be an indication of distant AMINO ACID SEQUENCE HOMOLOGY and used for rational DRUG DESIGN.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Communicable DiseasesComputational Biology: A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Plant Roots: The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)Phosphates: Inorganic salts of phosphoric acid.Fertilizers: Substances or mixtures that are added to the soil to supply nutrients or to make available nutrients already present in the soil, in order to increase plant growth and productivity.Indoleacetic Acids: Acetic acid derivatives of the heterocyclic compound indole. (Merck Index, 11th ed)Arabidopsis: A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.Gene Expression Regulation, Plant: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.Arabidopsis Proteins: Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments.Zea mays: A plant species of the family POACEAE. It is a tall grass grown for its EDIBLE GRAIN, corn, used as food and animal FODDER.Plants, Genetically Modified: PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.Phosphorus: A non-metal element that has the atomic symbol P, atomic number 15, and atomic weight 31. It is an essential element that takes part in a broad variety of biochemical reactions.Online Systems: Systems where the input data enter the computer directly from the point of origin (usually a terminal or workstation) and/or in which output data are transmitted directly to that terminal point of origin. (Sippl, Computer Dictionary, 4th ed)Information Services: Organized services to provide information on any questions an individual might have using databases and other sources. (From Random House Unabridged Dictionary, 2d ed)Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Consumer Health Information: Information intended for potential users of medical and healthcare services. There is an emphasis on self-care and preventive approaches as well as information for community-wide dissemination and use.Computer Communication Networks: A system containing any combination of computers, computer terminals, printers, audio or visual display devices, or telephones interconnected by telecommunications equipment or cables: used to transmit or receive information. (Random House Unabridged Dictionary, 2d ed)Information Storage and Retrieval: Organized activities related to the storage, location, search, and retrieval of information.Information Systems: Integrated set of files, procedures, and equipment for the storage, manipulation, and retrieval of information.User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Vaccines: Suspensions of killed or attenuated microorganisms (bacteria, viruses, fungi, protozoa), antigenic proteins, synthetic constructs, or other bio-molecular derivatives, administered for the prevention, amelioration, or treatment of infectious and other diseases.Databases as Topic: Organized collections of computer records, standardized in format and content, that are stored in any of a variety of computer-readable modes. They are the basic sets of data from which computer-readable files are created. (from ALA Glossary of Library and Information Science, 1983)Desulfovibrio vulgaris: A species of gram-negative, anaerobic, rod-shaped bacteria isolated from soil, animal intestines and feces, and fresh and salt water.Desulfovibrio: A genus of gram-negative, anaerobic, rod-shaped bacteria capable of reducing sulfur compounds to hydrogen sulfide. Organisms are isolated from anaerobic mud of fresh and salt water, animal intestines, manure, and feces.Staphylococcaceae: Family of gram-positive, facultatively anaerobic bacteria, in the order Bacillales. Genera include Gemella, Macrococcus, Salinicoccus, and STAPHYLOCOCCUS.Amino Acid Motifs: Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Hydrogenase: An enzyme found in bacteria. It catalyzes the reduction of FERREDOXIN and other substances in the presence of molecular hydrogen and is involved in the electron transport of bacterial photosynthesis.
(1/105) Molecular cloning and sequencing of the aroA gene from Actinobacillus pleuropneumoniae and its use in a PCR assay for rapid identification.

The gene (aroA) of Actinobacillus pleuropneumoniae, serotype 2, encoding 5-enolpyruvylshikimate-3-phosphate synthase was cloned by complementation of the aroA mutation in Escherichia coli K-12 strain AB2829, and the nucleotide sequence was determined. A pair of primers from the 5' and 3' termini were selected to be the basis for development of a specific PCR assay. A DNA fragment of 1,025 bp was amplified from lysed A. pleuropneumoniae serotypes 1 to 12 of biovar 1 or from isolated DNA. No PCR products were detected when chromosomal DNAs from other genera were used as target DNAs; however, a 1,025-bp DNA fragment was amplified when Actinobacillus equuli chromosomal DNA was used as a target, which could be easily differentiated by its NAD independence. The PCR assay developed was very sensitive, with lower detection limits of 12 CFU with A. pleuropneumoniae cells and 0.8 pg with extracted DNA. Specificity and sensitivity make this PCR assay a useful method for the rapid identification and diagnosis of A. pleuropneumoniae infections.  (+info)

(2/105) Evaluation of Salmonella typhimurium mutants in a model of experimental gastroenteritis.

Salmonella typhimurium strains harboring independent, defined mutations in aroA, invA, ssrA, or msbB were assessed for their ability to induce fluid accumulation, tissue damage, and local inflammation in rabbit ileal loops. Three wild-type strains of S. typhimurium, TML, HWSH, and SL1344, and two mutant strains, S. typhimurium SL1344 ssrA and S. typhimurium SL1344 msbB, consistently induced fluid accumulation in the lumen of loops and inflammation of loop-associated tissues. In contrast, three different S. typhimurium aroA strains and an invA mutant of SL1344 did not induce significant fluid accumulation in the rabbit ileal loops. However, the S. typhimurium aroA strains did induce an inflammatory infiltrate and some local villus-associated damage, but the invA mutant did not. Histologically, wild-type S. typhimurium, S. typhimurium SL1344 ssrA, and S. typhimurium SL1344 msbB demonstrated more severe effects on villus architecture than S. typhimurium aroA strains, whereas S. typhimurium invA-infected loops showed no detectable damage. This suggests that villus damage most likely contributes to fluid accumulation within the loop.  (+info)

(3/105) Characterization of Streptococcus pneumoniae 5-enolpyruvylshikimate 3-phosphate synthase and its activation by univalent cations.

The aroA gene (Escherichia coli nomenclature) encoding 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase from the gram-positive pathogen Streptococcus pneumoniae has been identified, cloned and overexpressed in E. coli, and the enzyme purified to homogeneity. It was shown to catalyze a reversible conversion of shikimate 3-phosphate (S3P) and phosphoenolpyruvate (PEP) to EPSP and inorganic phosphate. Activation by univalent cations was observed in the forward reaction, with NH+4, Rb+ and K+ exerting the greatest effects. Km(PEP) was lowered by increasing [NH+4] and [K+], whereas Km(S3P) rose with increasing [K+], but fell with increasing [NH+4]. Increasing [NH+4] and [K+] resulted in an overall increase in kcat. Glyphosate (GLP) was found to be a competitive inhibitor with PEP, but the potency of inhibition was profoundly affected by [NH+4] and [K+]. For example, increasing [NH+4] and [K+] reduced Ki(GLP versus PEP) up to 600-fold. In the reverse reaction, the enzyme catalysis was less sensitive to univalent cations. Our analysis included univalent cation concentrations comparable with those found in bacterial cells. Therefore, the observed effects of these metal ions are more likely to reflect the physiological behavior of EPSP synthase and also add to our understanding of how to inhibit this enzyme in the host organism. As there is a much evidence to suggest that EPSP synthase is essential for bacterial survival, its discovery in the serious gram-positive pathogen S. pneumoniae and its inhibition by GLP indicate its potential as a broad-spectrum antibacterial target.  (+info)

(4/105) Extending the CD4(+) T-cell epitope specificity of the Th1 immune response to an antigen using a Salmonella enterica serovar typhimurium delivery vehicle.

We analyzed the CD4 T-cell immunodominance of the response to a model antigen (Ag), MalE, when delivered by an attenuated strain of Salmonella enterica serovar Typhimurium (SL3261*pMalE). Compared to purified MalE Ag administered with adjuvant, the mapping of the peptide-specific proliferative responses showed qualitative differences when we used the Salmonella vehicle. We observed the disappearance of one out of eight MalE peptides' T-cell reactivity upon SL3261*pMalE immunization, but this phenomenon was probably due to a low level of T-cell priming, since it could be overcome by further immunization. The most striking effect of SL3261*pMalE administration was the activation and stimulation of new MalE peptide-specific T-cell responses that were silent after administration of purified Ag with adjuvant. Ag presentation assays performed with MalE-specific T-cell hybridomas showed that infection of Ag-presenting cells by this intracellular attenuated bacterium did not affect the processing and presentation of the different MalE peptides by major histocompatibility complex (MHC) class II molecules and therefore did not account for immunodominance modulation. Thus, immunodominance of the T-cell response to microorganisms is governed not only by the frequency of the available T-cell repertoire or the processing steps in Ag-presenting cells that lead to MHC presentation but also by other parameters probably related to the infectious process and to the bacterial products. Our results indicate that, upon infection by a microorganism, the specificity of the T-cell response induced against its Ags can be much more effective than with purified Ags and that it cannot completely be mimicked by purified Ags administered with adjuvant.  (+info)

(5/105) Comparison of abilities of Salmonella enterica serovar typhimurium aroA aroD and aroA htrA mutants to act as live vectors.

We compared the ability of Salmonella enterica serovar Typhimurium SL1344 aroA aroD (BRD509) and aroA htrA (BRD807) mutants to act as live vectors for delivery of fragment C of tetanus toxin (FrgC). FrgC was expressed in these strains from either pTETnir15 or pTEThtrA1. BRD509FrgC(+) strains elicited approximately 2-log-higher serum anti-FrgC antibody titers than BRD807FrgC(+) strains. All mice immunized with BRD807pTEThtrA1, BRD509pTEThtrA1, and BRD509pTETnir15 (but not BRD807pTETnir15) were protected against tetanus.  (+info)

(6/105) Interaction of the herbicide glyphosate with its target enzyme 5-enolpyruvylshikimate 3-phosphate synthase in atomic detail.

Biosynthesis of aromatic amino acids in plants, many bacteria, and microbes relies on the enzyme 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase, a prime target for drugs and herbicides. We have identified the interaction of EPSP synthase with one of its two substrates (shikimate 3-phosphate) and with the widely used herbicide glyphosate by x-ray crystallography. The two-domain enzyme closes on ligand binding, thereby forming the active site in the interdomain cleft. Glyphosate appears to occupy the binding site of the second substrate of EPSP synthase (phosphoenol pyruvate), mimicking an intermediate state of the ternary enzyme.substrates complex. The elucidation of the active site of EPSP synthase and especially of the binding pattern of glyphosate provides a valuable roadmap for engineering new herbicides and herbicide-resistant crops, as well as new antibiotic and antiparasitic drugs.  (+info)

(7/105) Genetic background of attenuated Salmonella typhimurium has profound influence on infection and cytokine patterns in human dendritic cells.

Salmonella typhimurium (ST) can cause infection in man, and attenuated strains are under consideration as live vaccine vectors. However, little is known about the interaction of ST with human dendritic cells (DC). Here, we compared the consequences of exposure of human, monocyte-derived DC with different attenuated strains of ST. Infection was observed with all four strains tested (wild type, PhoP-, PhoPc, and AroA), but the PhoPc strain was by far the most efficient. Intracellular persistence of wild type and PhoP- was longer than that of PhoPc and AroA, both of which were largely eliminated within 24 h. Most DC survived infection by the attenuated strains, although apoptosis was observed in a fraction of the exposed cells. All strains induced DC maturation, independent from the extent of infection. Although all strains stimulated secretion of TNF-alpha and IL-12 strongly, PhoPc induced significantly less IL-10 than the other three strains and as much as 10 times less IL-10 than heat-killed PhoPc, suggesting that this mutant suppressed the secretion of IL-10 by the DC. These data indicate that infectivity, bacterial elimination, and cytokine secretion in human DC are controlled by the genetic background of ST.  (+info)

(8/105) Chemical shift mapping of shikimate-3-phosphate binding to the isolated N-terminal domain of 5-enolpyruvylshikimate-3-phosphate synthase.

To facilitate evaluation of enzyme-ligand complexes in solution, we have isolated the 26-kDa N-terminal domain of 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase for analysis by NMR spectroscopy. The isolated domain is capable of binding the substrate shikimate-3-phosphate (S3P), and this letter reports the localization of the S3P binding site using chemical shift mapping. Based on the NMR data, we propose that Ser23, Arg27, Ser197, and Tyr200 are directly involved in S3P binding. We also describe changes in the observed nuclear Overhauser effects (NOEs) that are consistent with a partial conformational change in the N-terminal domain upon S3P binding.  (+info)

*  EPSP synthase
72 (1): 53-62. doi:10.1016/0168-9452(90)90186-r. Abdel-Meguid SS, Smith WW, Bild GS (Dec 1985). "Crystallization of 5- ... 87 (1): 232-8. doi:10.1104/pp.87.1.232. PMC 1054731 . PMID 16666109. Pollegioni L, Schonbrunn E, Siehl D (Aug 2011). "Molecular ... 186 (3): 673. doi:10.1016/0022-2836(85)90140-8. PMID 3912512. Ream JE, Steinrücken HC, Porter CA, Sikorski JA (May 1988). " ... 63 (1): 73-105. doi:10.1146/annurev-arplant-042811-105439. PMID 22554242. The AAA pathways consist of the shikimate pathway ( ...
*  Enzyme inhibitor
As such a term similar to the one proposed above to modulate Vmax should be appropriate in most situations: K m 1 − ( K m 1 − K ... 2 (1): 68-77. doi:10.1208/ps020108. PMC 2751003 . PMID 11741224. Loo JA, DeJohn DE, Du P, Stevenson TI, Ogorzalek Loo RR; ... 18 (1): 65-72. doi:10.1111/j.1432-1033.1971.tb01215.x. PMID 4322209. Reardon, JE (1989). "Herpes simplex virus type 1 and human ... 36 (1): 13-24. doi:10.1016/S0041-0101(97)00074-3. PMID 9604278. Holmes, CF; Maynes, JT; Perreault, KR; Dawson, JF; James, MN ( ...
*  List of MeSH codes (D08)
... glucan 1,4-beta-glucosidase MeSH D08.811.277.450.420.200.600 --- glucan endo-1,3-beta-d-glucosidase MeSH D08.811.277.450. ... endo-1,4-beta xylanases MeSH D08.811.277.450.950.500 --- xylan endo-1,3-beta-xylosidase MeSH D08.811.277.656.149 --- atp- ... glycogen synthase kinase 3 MeSH D08.811.913.696.620.682.700.494 --- i-kappa B kinase MeSH D08.811.913.696.620.682.700.559 --- ... map kinase kinase 1 MeSH D08.811.913.696.620.682.700.565.200 --- map kinase kinase 2 MeSH D08.811.913.696.620.682.700.565.300 ...
*  List of EC numbers (EC 2)
3-deoxy-D-manno-octulosonic acid kinase EC 2.7.1.167: D-glycero-beta-D-manno-heptose-7-phosphate kinase EC 2.7.1.168: D-glycero ... D-inositol-3-phosphate glycosyltransferase EC 2.4.1.251: GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1- ... inositol 3-a-galactosyltransferase EC 2.4.1.124: now included with EC 2.4.1.87 EC 2.4.1.125: sucrose-1,6-a-glucan 3(6)-a- ... 4-benzoxazin-3(4H)-one 2 -D-glucosyltransferase EC 2.4.1.203: trans-zeatin O-b-D-glucosyltransferase EC 2.4.1.204: now EC 2.4. ...
RCSB PDB 









- 3SLH: 1.70 Angstrom resolution structure of 3-phosphoshikimate 1-carboxyvinyltransferase (AroA) from...  RCSB PDB - 3SLH: 1.70 Angstrom resolution structure of 3-phosphoshikimate 1-carboxyvinyltransferase (AroA) from...
1.70 Angstrom resolution structure of 3-phosphoshikimate 1-carboxyvinyltransferase(AroA) from Coxiella burnetii in complex with ... 3-phosphoshikimate 1-carboxyvinyltransferase A, B, C, D 441 Coxiella burnetii EC#: 2.5.1.19 IUBMB Gene Name(s): aroA CBU_0526 ... 3R,4S,5R)-3,4,5-TRIHYDROXYCYCLOHEX-1-ENE- 1-CARBOXYLIC ACID. SHIKIMATE (Synonym). C7 H10 O5 JXOHGGNKMLTUBP-HSUXUTPPSA-N ... 1,2-ETHANEDIOL. ETHYLENE GLYCOL (Synonym). C2 H6 O2 LYCAIKOWRPUZTN-UHFFFAOYSA-N ...
more infohttp://www.rcsb.org/pdb/explore/explore.do?structureId=3slh
RCSB PDB - 3SLH: 1.70 Angstrom resolution structure of 3-phosphoshikimate 1-carboxyvinyltransferase (AroA) from Coxiella...  RCSB PDB - 3SLH: 1.70 Angstrom resolution structure of 3-phosphoshikimate 1-carboxyvinyltransferase (AroA) from Coxiella...
1.70 Angstrom resolution structure of 3-phosphoshikimate 1-carboxyvinyltransferase (AroA) from Coxiella burnetii in complex ... 3R,4S,5R)-3,4,5-TRIHYDROXYCYCLOHEX-1-ENE-1-CARBOXYLIC ACID. SHIKIMATE. C7 H10 O5. JXOHGGNKMLTUBP-HSUXUTPPSA-N. ... 3-phosphoshikimate 1-carboxyvinyltransferase. A, B, C, D. 441. Coxiella burnetii (strain RSA 493 / Nine Mile phase I). Gene ... 1,2-ETHANEDIOL. ETHYLENE GLYCOL. C2 H6 O2. LYCAIKOWRPUZTN-UHFFFAOYSA-N. ...
more infohttps://www.rcsb.org/structure/3slh
aroA recombinant protein | 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) Recombinant Protein-WP 004685324.1  aroA recombinant protein | 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) Recombinant Protein-WP 004685324.1
3-phosphoshikimate 1-carboxyvinyltransferase (aroA) Recombinant Protein-WP_004685324.1 (MBS1047607) product datasheet at ... 0.05 mg (E-Coli) / $870 +1 FREE 8GB USB. 0.05 mg (Yeast) / $940 +1 FREE 8GB USB. 0.2 mg (E-Coli) / $1,160 +1 FREE 8GB USB. 0.05 ... 1 mg (E-Coli) / $1,940 +2 FREE 8GB USB. 0.5 mg (Baculovirus) / $2,200 +2 FREE 8GB USB. 0.1 mg (Mammalian-Cell) / $2,285 +2 FREE ... 0.2 mg (Yeast) / $1,275 +1 FREE 8GB USB. 0.5 mg (E-Coli) / $1,280 +1 FREE 8GB USB. 0.05 mg (Mammalian-Cell) / $1,410 +1 FREE ...
more infohttps://www.mybiosource.com/aroa-recombinant-protein/3-phosphoshikimate-1-carboxyvinyltransferase-aroa/1047607
aroA recombinant protein | 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) Recombinant Protein-WP 000245895.1  aroA recombinant protein | 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) Recombinant Protein-WP 000245895.1
3-phosphoshikimate 1-carboxyvinyltransferase (aroA) Recombinant Protein-WP_000245895.1 (MBS1381469) product datasheet at ... 1 mg (E-Coli) / $1,895 +2 FREE 8GB USB. 0.5 mg (Baculovirus) / $2,170 +2 FREE 8GB USB. 1 mg (Yeast) / $2,255 +2 FREE 8GB USB. ... 0.05 mg (E-Coli) / $850 +1 FREE 8GB USB. 0.05 mg (Yeast) / $925 +1 FREE 8GB USB. 0.2 mg (E-Coli) / $1,135 +1 FREE 8GB USB. 0.05 ... 0.5 mg (E-Coli) / $1,250 +1 FREE 8GB USB. 0.2 mg (Yeast) / $1,255 +1 FREE 8GB USB. 0.05 mg (Mammalian-Cell) / $1,395 +1 FREE ...
more infohttps://www.mybiosource.com/aroa-recombinant-protein/3-phosphoshikimate-1-carboxyvinyltransferase-aroa/1381469
Proteins matched: EPSP SYNTHASE 2 (PS00885) | InterPro | EMBL-EBI  Proteins matched: EPSP SYNTHASE 2 (PS00885) | InterPro | EMBL-EBI
3-phosphoshikimate 1-carboxyvinyltransferase. Magnetococcus marinus (strain ATCC BAA-1437 / JCM 17883 / MC-1). Loading... ... 3-phosphoshikimate 1-carboxyvinyltransferase. Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334). ... 3-phosphoshikimate 1-carboxyvinyltransferase. Methanothrix thermoacetophila (strain DSM 6194 / JCM 14653 / NBRC 101360 / PT). ... 3-phosphoshikimate 1-carboxyvinyltransferase. Pelobacter propionicus (strain DSM 2379 / NBRC 103807 / OttBd1). Loading... ...
more infohttp://www.ebi.ac.uk/interpro/ISignatureProteins?sig=PS00885&query=P
EC 2.5.1.1-50  EC 2.5.1.1-50
EC 2.5.1. See separate file for EC 2.5.1.51 to EC 2.5.1.100 and EC 2.5.1.101 to EC 2.5.1.151 Contents. EC 2.5.1.1 dimethylallyl ... Int. 3 (1981) 669-675.. [EC 2.5.1.29 created 1984, modified 2011]. EC 2.5.1.30. Accepted name: heptaprenyl diphosphate synthase ... Glossary: O-acetyl-L-serine = (2S)-3-acetyloxy-2-aminopropanoic acid. Other name(s): O-acetyl-L-serine sulfhydrylase; O-acetyl- ... putrescine = butane-1,4-diamine. S-adenosyl 3-(methylsulfanyl)propylamine = (3-aminopropyl){[(2S,3S,4R,5R)-5-(6-amino-9H-purin- ...
more infohttps://www.qmul.ac.uk/sbcs/iubmb/enzyme/EC2/05.html
Biological characterization of Pasteurella multocida present in the Saiga population | SpringerLink  Biological characterization of Pasteurella multocida present in the Saiga population | SpringerLink
1.. Open image in new window. Fig. 1 Capsular typing of the strains. Explanation: Figure showing positive controls working on ... 1.. Biberstein EL. The pasteurelloses. In: Handbook series in zoonoses, H. Stoenner, M. Torten and W. Kaplan (eds.). CRC Press ... 1.Research Institute for Biological Safety ProblemsMinistry of Education and Science of Republic of Kazakhstan - Science ... 3.Research Institute of Wildlife EcologyUniversity of Veterinary Medicine ViennaViennaAustria ...
more infohttps://link.springer.com/article/10.1186%2Fs12866-019-1407-9
Confirmation of RNA-seq results. (A) Bar plot of edgeR  | Open-i  Confirmation of RNA-seq results. (A) Bar plot of edgeR | Open-i
... associated receptor kinase 1, pecmet is pectin methylesterase, serthre is receptor serine-threonine protein, vichyd is vicianin ... associated receptor kinase 1, pecmet is pectin methylesterase, serthre is receptor serine-threonine protein, vichyd is vicianin ... and a brassinosteroid insensitive 1-associated receptor kinase (Table 4). There was no evidence that the scaffold identified as ... and a brassinosteroid insensitive 1-associated receptor kinase (Table 4). There was no evidence that the scaffold identified as ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC4199709_2035f7&req=4
Center for Structural Genomics of Infectious Diseases - Deposits  Center for Structural Genomics of Infectious Diseases - Deposits
... carboxyvinyltransferase is the sixth enzyme in the shikimate pathway and catalyzes the conversion of shikimate-3-phosphate and ... P 1 21 1 Unit Cell. a=46.25Å, b=186.64Å, c=95.79Å. α=90.00, β=91.51, γ=90.00 Solvent content. Matthews coefficient. Refinement ... This protein was co-crystallized with the substrate shikimate-3-phosphate and the commercially successful herbicide glyphosate ... Glyphosate binds adjacent to shikimate-3-phosphate in phosphoenolpyruvate's binding site. Interestingly, two C-terminal apo ...
more infohttps://csgid.org/deposits/view/3SLH
Differential gene expression in an elite hybrid rice cultivar (Oryza sativa, L) and its parental lines based on SAGE data | BMC...  Differential gene expression in an elite hybrid rice cultivar (Oryza sativa, L) and its parental lines based on SAGE data | BMC...
2005, 3 (2): e38-10.1371/journal.pbio.0030038.PubMedPubMed CentralView ArticleGoogle Scholar. ... 2004, 134 (1): 67-80. 10.1104/pp.103.030536.PubMedPubMed CentralView ArticleGoogle Scholar. ... 2005, 138 (3): 1216-1231. 10.1104/pp.105.060988.PubMedPubMed CentralView ArticleGoogle Scholar. ... 2007, 17 (1): 108-116. 10.1101/gr.5488207.PubMedPubMed CentralView ArticleGoogle Scholar. ...
more infohttps://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-7-49
aroA recombinant protein product blog  aroA recombinant protein product blog
Tags: Recombinant Protein; aroA recombinant protein; aroA; 3-phosphoshikimate 1-carboxyvinyltransferase (aroA); ...
more infohttp://rec.mybiosource.com/aroa-recombinant-protein_1381469
Center for Structural Genomics of Infectious Diseases - Pages  Center for Structural Genomics of Infectious Diseases - Pages
... carboxyvinyltransferase from Vibrio cholerae in complex with shikimate-3-phosphate (partially photolyzed) and glyphosate. ... Title: Crystal structure of aminoglycoside acetyltransferase AAC(3)-Ib. Authors: P.J.Stogios,Z.Xu,E.Evdokimova,V.Yim,W.F. ... Title: 2.75 Angstrom Crystal Structure of Enolase 1 from Toxoplasma gondii.. Authors: G.Minasov,J.Ruan,L.Shuvalova,A.Halavaty,H ... Title: X-Ray Crystal Structure of a Fragment (1-75) of a Transcriptional Regulator PdhR from Escherichia coli CFT073 ...
more infohttps://csgid.org/pages/community_request_targets/
Phosphate starvation of maize inhibits lateral root formation and alters gene expression in the lateral root primordium zone |...  Phosphate starvation of maize inhibits lateral root formation and alters gene expression in the lateral root primordium zone |...
10.1007/s00425-011-1368-1.PubMedView ArticleGoogle Scholar. *. Devaiah BN, Karthikeyan AS, Raghothama KG: WRKY75 transcription ... NO3)2.4H2O, 1.25 mM NH4NO3, 0.1 mM KCl, 0.65 mM K2SO4, 0.65 mM MgSO4, 10.0 mM H3BO3, 0.5 mM (NH4)6Mo7O24, 1.0 mM MnSO4, 0.1 mM ... As shown in Table 1 and Figure 1, after 3 days of LP treatment, the Pi contents in both roots and shoots had decreased slightly ... After 2-3 days of re-culturing in SP nutrient solutions, half of the seedlings were transplanted into a low-phosphate (LP, same ...
more infohttps://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-12-89
An improved probability mapping approach to assess genome mosaicism | BMC Genomics | Full Text  An improved probability mapping approach to assess genome mosaicism | BMC Genomics | Full Text
2002, 3: 4-10.1186/1471-2164-3-4.PubMed CentralView ArticlePubMedGoogle Scholar. ... Table 1 summarizes these findings. There are many datasets which don't conform to the 16S rRNA based expectation in more than ... Figure 3 Maps of bootstrap support from extended datasets for genome quartets containing Synechocystis sp. The three numbers ... A1) between a mesophilic bacterium and a mesophilic archaeon. A2) between the extremely mesophilic bacteria Aquifex and ...
more infohttps://0-bmcgenomics-biomedcentral-com.brum.beds.ac.uk/articles/10.1186/1471-2164-4-37
aroC protein (Sulfurovum sp. NBC371) - STRING interaction network  aroC protein (Sulfurovum sp. NBC371) - STRING interaction network
Ribonuclease 3; Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate ... 3-dehydroquinate synthase; Catalyzes the conversion of 3-deoxy-D-arabino- heptulosonate 7-phosphate (DAHP) to dehydroquinate ( ... 3-dehydroquinate synthase; Catalyzes the conversion of 3-deoxy-D-arabino- heptulosonate 7-phosphate (DAHP) to dehydroquinate ( ... 3-dehydroquinate synthase; Catalyzes the conversion of 3-deoxy-D-arabino- heptulosonate 7-phosphate (DAHP) to dehydroquinate ( ...
more infohttps://string-db.org/network/387093.SUN_2260
VIOLIN: Vaccine Investigation and Online Information Network  VIOLIN: Vaccine Investigation and Online Information Network
3. Mu et al., 2011: Mu W, Guan L, Yan Y, Liu Q, Zhang Y. A novel in vivo inducible expression system in Edwardsiella tarda for ... 1. Hernanz et al., 1998: Hernanz Moral C, Flaño del Castillo E, López Fierro P, Villena Cortés A, Anguita Castillo J, Cascón ... Protein Note : Also known as NAF; GCP1; LECT; LUCT; NAP1; CXCL8; GCP-1; LYNAP; MDNCF; MONAP; NAP-1 ... gi,225007536,ref,NP_001139345.1, T-cell surface glycoprotein CD8 alpha chain isoform 1 precursor [Homo sapiens] ...
more infohttp://www.violinet.org/vaxquery/query_detail.php?c_pathogen_id=211
Acin 0027 protein (Acidaminococcus intestini) - STRING interaction network  Acin 0027 protein (Acidaminococcus intestini) - STRING interaction network
Phospho-2-dehydro-3-deoxyheptonate aldolase. Chorismate synthase; Catalyzes the anti-1,4-elimination of the C-3 phosphate and ... Phospho-2-dehydro-3-deoxyheptonate aldolase. Shikimate kinase; Catalyzes the specific phosphorylation of the 3-hydroxyl group ... Phospho-2-dehydro-3-deoxyheptonate aldolase. Shikimate kinase; Catalyzes the specific phosphorylation of the 3-hydroxyl group ... Phospho-2-dehydro-3-deoxyheptonate aldolase. Shikimate dehydrogenase; Involved in the biosynthesis of the chorismate, which ...
more infohttps://string-db.org/network/568816.Acin_0027
Subject: Ochrobactrum - PubAg Search Results  Subject: 'Ochrobactrum' - PubAg Search Results
3 Biotech 2019 v.9 no.2 pp. 52. ISSN:. 2190-572X. Subject:. Ochrobactrum anthropi, etc ; biodegradation; carbon; catechol; ... 3 Biotech 2019 v.9 no.3 pp. 74. ISSN:. 2190-572X. Subject:. Ochrobactrum, etc ; 3-phosphoshikimate 1-carboxyvinyltransferase; ... 3. Simultaneous nitrification and denitrification without nitrite accumulation by a novel isolated Ochrobactrum anthropic LJ81 ... 1. Biosorption behavior of the Ochrobactrum MT180101 on ionic copper and chelate copper ...
more infohttps://pubag.nal.usda.gov/?q=%22Ochrobactrum%22&
VIOLIN: Vaccine Investigation and Online Information Network  VIOLIN: Vaccine Investigation and Online Information Network
1. Bagnoli et al., 2015: Bagnoli F, Fontana MR, Soldaini E, Mishra RP, Fiaschi L, Cartocci E, Nardi-Dei V, Ruggiero P, Nosari S ... 2005; 45(1); 45-51. [PubMed: 15985222].. 6. Delfani et al., 2016: Delfani S, Mohabati Mobarez A, Imani Fooladi AA, Amani J, ... 3. Castagliuolo et al., 2006: Castagliuolo I, Piccinini R, Beggiao E, Palù G, Mengoli C, Ditadi F, Vicenzoni G, Zecconi A. ... 3. Introduction Staphylococcus aureus is a facultatively anaerobic, gram-positive coccus and is the most common cause of staph ...
more infohttp://www.violinet.org/vaxquery/query_detail.php?c_pathogen_id=138
Network Portal - Gene DVU0123  Network Portal - Gene DVU0123
POSITION A C G T 1 1.0 0.0 0.0 0.0 2 1.0 0.0 0.0 0.0 3 1.0 0.0 0.0 0.0 4 0.8 0.0 0.0 0.2 5 0.0 0.6 0.4 0.0 6 0.4 0.0 0.0 0.6 7 ... POSITION A C G T 1 0.0 0.8 0.0 0.2 2 1.0 0.0 0.0 0.0 3 0.0 1.0 0.0 0.0 4 0.0 0.4 0.6 0.0 5 0.0 0.0 0.0 1.0 6 0.8 0.2 0.0 0.0 7 ... 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this ... 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this ...
more infohttp://networks.systemsbiology.net/dvu/gene/DVU0123