3-Deoxy-7-Phosphoheptulonate Synthase: An enzyme that catalyzes the formation of 7-phospho-2-keto-3-deoxy-D-arabinoheptonate from phosphoenolpyruvate and D-erythrose-4-phosphate. It is one of the first enzymes in the biosynthesis of TYROSINE and PHENYLALANINE. This enzyme was formerly listed as EC 4.1.2.15.Deoxy SugarsHemoglobin A: Normal adult human hemoglobin. The globin moiety consists of two alpha and two beta chains.Citrate (si)-Synthase: Enzyme that catalyzes the first step of the tricarboxylic acid cycle (CITRIC ACID CYCLE). It catalyzes the reaction of oxaloacetate and acetyl CoA to form citrate and coenzyme A. This enzyme was formerly listed as EC 4.1.3.7.CarboxyhemoglobinGlycogen Synthase: An enzyme that catalyzes the transfer of D-glucose from UDPglucose into 1,4-alpha-D-glucosyl chains. EC 2.4.1.11.Chromomycin A3: Glycosidic antibiotic from Streptomyces griseus used as a fluorescent stain of DNA and as an antineoplastic agent.Odontogenic Cysts: Cysts found in the jaws and arising from epithelium involved in tooth formation. They include follicular cysts (e.g., primordial cyst, dentigerous cyst, multilocular cyst), lateral periodontal cysts, and radicular cysts. They may become keratinized (odontogenic keratocysts). Follicular cysts may give rise to ameloblastomas and, in rare cases, undergo malignant transformation.Reticular Formation: A region extending from the PONS & MEDULLA OBLONGATA through the MESENCEPHALON, characterized by a diversity of neurons of various sizes and shapes, arranged in different aggregations and enmeshed in a complicated fiber network.Search Engine: Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.Oxidoreductases, N-DemethylatingInformation Storage and Retrieval: Organized activities related to the storage, location, search, and retrieval of information.Semiconductors: Materials that have a limited and usually variable electrical conductivity. They are particularly useful for the production of solid-state electronic devices.Desulfovibrio vulgaris: A species of gram-negative, anaerobic, rod-shaped bacteria isolated from soil, animal intestines and feces, and fresh and salt water.Desulfovibrio: A genus of gram-negative, anaerobic, rod-shaped bacteria capable of reducing sulfur compounds to hydrogen sulfide. Organisms are isolated from anaerobic mud of fresh and salt water, animal intestines, manure, and feces.Staphylococcaceae: Family of gram-positive, facultatively anaerobic bacteria, in the order Bacillales. Genera include Gemella, Macrococcus, Salinicoccus, and STAPHYLOCOCCUS.Computational Biology: A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.Amino Acid Motifs: Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.Pyrococcus furiosus: A species of strictly anaerobic, hyperthermophilic archaea which lives in geothermally-heated marine sediments. It exhibits heterotropic growth by fermentation or sulfur respiration.Aldehyde-Lyases: Enzymes that catalyze a reverse aldol condensation. A molecule containing a hydroxyl group and a carbonyl group is cleaved at a C-C bond to produce two smaller molecules (ALDEHYDES or KETONES). EC 4.1.2.Pyrococcus: A genus of strictly anaerobic ultrathermophilic archaea, in the family THERMOCOCCACEAE, occurring in heated seawaters. They exhibit heterotrophic growth at an optimum temperature of 100 degrees C.Phenylalanine: An essential aromatic amino acid that is a precursor of MELANIN; DOPAMINE; noradrenalin (NOREPINEPHRINE), and THYROXINE.Myo-Inositol-1-Phosphate Synthase: An enzyme that catalyzes the formation of myo-inositol-1-phosphate from glucose-6-phosphate in the presence of NAD. EC 5.5.1.4.Pyrococcus horikoshii: Anaerobic hyperthermophilic species of ARCHAEA, isolated from hydrothermal fluid samples. It is obligately heterotrophic with coccoid cells that require TRYPTOPHAN for growth.Hexosaminidases: Enzymes that catalyze the hydrolysis of N-acylhexosamine residues in N-acylhexosamides. Hexosaminidases also act on GLUCOSIDES; GALACTOSIDES; and several OLIGOSACCHARIDES.Man-Machine Systems: A system in which the functions of the man and the machine are interrelated and necessary for the operation of the system.5,10-Methylenetetrahydrofolate Reductase (FADH2): An FAD-dependent oxidoreductase found primarily in BACTERIA. It is specific for the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate. This enzyme was formerly listed as EC 1.1.1.68 and 1.1.99.15.Pterygoid Muscles: Two of the masticatory muscles: the internal, or medial, pterygoid muscle and external, or lateral, pterygoid muscle. Action of the former is closing the jaws and that of the latter is opening the jaws, protruding the mandible, and moving the mandible from side to side.Neurospora crassa: A species of ascomycetous fungi of the family Sordariaceae, order SORDARIALES, much used in biochemical, genetic, and physiologic studies.Nicotinamide N-Methyltransferase: An enzyme found primarily in the LIVER that catalyzes the N-methylation of NICOTINAMIDE and other structurally related compounds.Guanidinoacetate N-Methyltransferase: This enzyme catalyzes the last step of CREATINE biosynthesis by catalyzing the METHYLATION of guanidinoacetate to CREATINE.Histamine N-Methyltransferase: An enzyme that catalyzes the transfer of a methyl group from S-adenosylmethionine to histamine, forming N-methylhistamine, the major metabolite of histamine in man. EC 2.1.1.8.Glycine N-Methyltransferase: An enzyme that catalyzes the METHYLATION of GLYCINE using S-ADENOSYLMETHIONINE to form SARCOSINE with the concomitant production of S-ADENOSYLHOMOCYSTEINE.Phosphatidylethanolamine N-Methyltransferase: An enzyme that catalyses three sequential METHYLATION reactions for conversion of phosphatidylethanolamine to PHOSPHATIDYLCHOLINE.Betaine-Homocysteine S-Methyltransferase: A ZINC metalloenzyme that catalyzes the transfer of a methyl group from BETAINE to HOMOCYSTEINE to produce dimethylglycine and METHIONINE, respectively. This enzyme is a member of a family of ZINC-dependent METHYLTRANSFERASES that use THIOLS or selenols as methyl acceptors.Methyltransferases: A subclass of enzymes of the transferase class that catalyze the transfer of a methyl group from one compound to another. (Dorland, 28th ed) EC 2.1.1.

Metal-catalyzed oxidation of phenylalanine-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Escherichia coli: inactivation and destabilization by oxidation of active-site cysteines. (1/95)

The in vitro instability of the phenylalanine-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase [DAHPS(Phe)] from Escherichia coli has been found to be due to a metal-catalyzed oxidation mechanism. DAHPS(Phe) is one of three differentially feedback-regulated isoforms of the enzyme which catalyzes the first step of aromatic biosynthesis, the formation of DAHP from phosphoenolpyruvate and D-erythrose-4-phosphate. The activity of the apoenzyme decayed exponentially, with a half-life of about 1 day at room temperature, and the heterotetramer slowly dissociated to the monomeric state. The enzyme was stabilized by the presence of phosphoenolpyruvate or EDTA, indicating that in the absence of substrate, a trace metal(s) was the inactivating agent. Cu2+ and Fe2+, but none of the other divalent metals that activate the enzyme, greatly accelerated the rate of inactivation and subunit dissociation. Both anaerobiosis and the addition of catalase significantly reduced Cu2+-catalyzed inactivation. In the spontaneously inactivated enzyme, there was a net loss of two of the seven thiols per subunit; this value increased with increasing concentrations of added Cu2+. Dithiothreitol completely restored the enzymatic activity and the two lost thiols in the spontaneously inactivated enzyme but was only partially effective in reactivation of the Cu2+-inactivated enzyme. Mutant enzymes with conservative replacements at either of the two active-site cysteines, Cys61 or Cys328, were insensitive to the metal attack. Peptide mapping of the Cu2+-inactivated enzyme revealed a disulfide linkage between these two cysteine residues. All results indicate that DAHPS(Phe) is a metal-catalyzed oxidation system wherein bound substrate protects active-site residues from oxidative attack catalyzed by bound redox metal cofactor. A mechanism of inactivation of DAHPS is proposed that features a metal redox cycle that requires the sequential oxidation of its two active-site cysteines.  (+info)

UV light selectively coinduces supply pathways from primary metabolism and flavonoid secondary product formation in parsley. (2/95)

The UV light-induced synthesis of UV-protective flavonoids diverts substantial amounts of substrates from primary metabolism into secondary product formation and thus causes major perturbations of the cellular homeostasis. Results from this study show that the mRNAs encoding representative enzymes from various supply pathways are coinduced in UV-irradiated parsley cells (Petroselinum crispum) with two mRNAs of flavonoid glycoside biosynthesis, encoding phenylalanine ammonia-lyase and chalcone synthase. Strong induction was observed for mRNAs encoding glucose 6-phosphate dehydrogenase (carbohydrate metabolism, providing substrates for the shikimate pathway), 3-deoxyarabinoheptulosonate 7-phosphate synthase (shikimate pathway, yielding phenylalanine), and acyl-CoA oxidase (fatty acid degradation, yielding acetyl-CoA), and moderate induction for an mRNA encoding S-adenosyl-homocysteine hydrolase (activated methyl cycle, yielding S-adenosyl-methionine for B-ring methylation). Ten arbitrarily selected mRNAs representing various unrelated metabolic activities remained unaffected. Comparative analysis of acyl-CoA oxidase and chalcone synthase with respect to mRNA expression modes and gene promoter structure and function revealed close similarities. These results indicate a fine-tuned regulatory network integrating those functionally related pathways of primary and secondary metabolism that are specifically required for protective adaptation to UV irradiation. Although the response of parsley cells to UV light is considerably broader than previously assumed, it contrasts greatly with the extensive metabolic reprogramming observed previously in elicitor-treated or fungus-infected cells.  (+info)

Expression of tryptophan decarboxylase and tyrosine decarboxylase genes in tobacco results in altered biochemical and physiological phenotypes. (3/95)

The substrate specificity of tryptophan (Trp) decarboxylase (TDC) for Trp and tyrosine (Tyr) decarboxylase (TYDC) for Tyr was used to modify the in vivo pools of these amino acids in transgenic tobacco. Expression of TDC and TYDC was shown to deplete the levels of Trp and Tyr, respectively, during seedling development. The creation of artificial metabolic sinks for Trp and Tyr also drastically affected the levels of phenylalanine, as well as those of the non-aromatic amino acids methionine, valine, and leucine. Transgenic seedlings also displayed a root-curling phenotype that directly correlated with the depletion of the Trp pool. Non-transformed control seedlings could be induced to display this phenotype after treatment with inhibitors of auxin translocation such as 2,3,5-triiodobenzoic acid or N-1-naphthylphthalamic acid. The depletion of aromatic amino acids was also correlated with increases in the activities of the shikimate and phenylpropanoid pathways in older, light-treated transgenic seedlings expressing TDC, TYDC, or both. These results provide in vivo confirmation that aromatic amino acids exert regulatory feedback control over carbon flux through the shikimate pathway, as well as affecting pathways outside of aromatic amino acid biosynthesis.  (+info)

Histidine 268 in 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase plays the same role as histidine 202 in 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase. (4/95)

The enzyme 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAH 7-P) synthase (Phe) is inactivated by diethyl pyrocarbonate (DEPC). The inactivation is first order with respect to enzyme and DEPC concentrations with a pseudo-second order rate constant of inactivation by DEPC of 4.9 +/- 0.8 m(-1) s(-1) at pH 6.8 and 4 degrees C. The dependence of inactivation on pH and the spectral features of enzyme modified at specific pH values imply that both histidine and cysteine residues are modified, which is confirmed by site-directed mutagenesis. Analysis of the chemical modification data indicates that one histidine is essential for activity. DAH 7-P synthase (Phe) is protected against DEPC inactivation by phosphoenolpyruvate, whereas d-erythrose 4-phosphate offers only minimal protection. The conserved residues H-172, H-207, H-268, and H-304 were individually mutated to glycine. The H304G and H207G mutants retain some level of activity, whereas the H268G and H172G mutants are virtually inactive. A comparison of the circular dichroism spectra of wild-type enzyme and the various mutants demonstrates that H-172 may play a structural role. Comparison of the UV spectra of the H268G and wild-type enzymes saturated with Cu(2+) indicates that the metal-binding site of the H268G mutant resembles that of the wild-type enzyme. The residue H-268 may play a catalytic role based on the site-directed mutagenesis and spectroscopic studies. Cysteine 61 appears to influence the pK(a) of H-268 in the wild-type enzyme. The pK(a) of H-268 increases from 6.0 to 7.0 following mutation of C-61 to glycine.  (+info)

Microbial origin of plant-type 2-keto-3-deoxy-D-arabino-heptulosonate 7-phosphate synthases, exemplified by the chorismate- and tryptophan-regulated enzyme from Xanthomonas campestris. (5/95)

Enzymes performing the initial reaction of aromatic amino acid biosynthesis, 2-keto-3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthases, exist as two distinct homology classes. The three classic Escherichia coli paralogs are AroA(I) proteins, but many members of the Bacteria possess the AroA(II) class of enzyme, sometimes in combination with AroA(I) proteins. AroA(II) DAHP synthases until now have been shown to be specifically dedicated to secondary metabolism (e.g., formation of ansamycin antibiotics or phenazine pigment). In contrast, here we show that the Xanthomonas campestris AroA(II) protein functions as the sole DAHP synthase supporting aromatic amino acid biosynthesis. X. campestris AroA(II) was cloned in E. coli by functional complementation, and genes corresponding to two possible translation starts were expressed. We developed a 1-day partial purification method (>99%) for the unstable protein. The recombinant AroA(II) protein was found to be subject to an allosteric pattern of sequential feedback inhibition in which chorismate is the prime allosteric effector. L-Tryptophan was found to be a minor feedback inhibitor. An N-terminal region of 111 amino acids may be located in the periplasm since a probable inner membrane-spanning region is predicted. Unlike chloroplast-localized AroA(II) of higher plants, X. campestris AroA(II) was not hysteretically activated by dithiols. Compared to plant AroA(II) proteins, differences in divalent metal activation were also observed. Phylogenetic tree analysis shows that AroA(II) originated within the Bacteria domain, and it seems probable that higher-plant plastids acquired AroA(II) from a gram-negative bacterium via endosymbiosis. The X. campestris AroA(II) protein is suggested to exemplify a case of analog displacement whereby an ancestral aroA(I) species was discarded, with the aroA(II) replacement providing an alternative pattern of allosteric control. Three subgroups of AroA(II) proteins can be recognized: a large, central group containing the plant enzymes and that from X. campestris, one defined by a three-residue deletion near the conserved KPRS motif, and one possessing a larger deletion further downstream.  (+info)

The ARO4 gene of Candida albicans encodes a tyrosine-sensitive DAHP synthase: evolution, functional conservation and phenotype of Aro3p-, Aro4p-deficient mutants. (6/95)

The enzyme 3-deoxy-D-arabinoheptulosonate-7-phosphate (DAHP) synthase catalyses the first step in aromatic amino acid biosynthesis in prokaryotes, plants and fungi. Cells of Saccharomyces cerevisiae contain two catalytically redundant DAHP synthases, encoded by the genes ARO3 and ARO4, whose activities are feedback-inhibited by phenylalanine and tyrosine, respectively. ARO3/4 gene transcription is controlled by GCN4. The authors previously cloned an ARO3 gene orthologue from Candida albicans and found that: (1) it can complement an aro3 aro4 double mutation in S. cerevisiae, an effect inhibited by excess phenylalanine, and (2) a homozygous aro3-deletion mutant of C. albicans is phenotypically Aro(+), suggesting the existence of another isozyme(s). They now report the identification and functional characterization of the C. albicans orthologue of S. cerevisiae Aro4p. The two Aro4p enzymes share 68% amino acid identity. Phylogenetic analysis places the fungal DAHP synthases in a cluster separate from prokaryotic orthologues and suggests that ARO3 and ARO4 arose from a single gene via a gene duplication event early in fungal evolution. C. albicans ARO4 mRNA is elevated upon amino acid starvation, consistent with the presence of three putative Gcn4p-responsive elements (GCREs) in the gene promoter sequence. C. albicans ARO4 complements an aro3 aro4 double mutation in S. cerevisiae, an effect inhibited by excess tyrosine. The authors engineered Deltaaro3/Deltaaro3 Deltaaro4/MET3p::ARO4 cells of C. albicans (with one wild-type copy of ARO4 placed under control of the repressible MET3 promoter) and found that they fail to grow in the absence of aromatic amino acids when ARO4 expression is repressed, and that this growth defect can be partially rescued by aromatic amino acids and certain aromatic amino acid pathway intermediates. It is concluded that, like S. cerevisiae, C. albicans contains two DAHP synthases required for the first step in the aromatic amino acid biosynthetic pathway.  (+info)

Properties of tyrosine-inhibitable 3-deoxy-d-arabinoheptulosonic acid-7-phosphate synthase from Salmonella. (7/95)

Tyrosine-inhibitable 3-deoxy-D-arabinoheptulosonic acid-7-phosphate (DAHP) synthase was purified to homogeneity without significant loss of sensitivity to inhibition by tyrosine from an operator-constitutive strain (tyrOc) of Salmonella. The enzyme had an apparent molecular weight of 76,000 by gel filtration and a subunit molecular weight of 40,000 by sodium dodecyl sulfate-gel electrophoresis and by reaction with dimethyl suberimidate. It had an isoelectric point of 4.68. Inhibition by L-tyrosine showed a Hill coefficient of 1.8 at pH 7.0, suggesting cooperative interaction between tyrosine-binding sites, and was competitive with phosphoenol pyruvate and noncompetitive with erythrose-4-phosphate.  (+info)

Redox regulation of Arabidopsis 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase. (8/95)

The cDNA for 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase of Arabidopsis encodes a polypeptide with an amino-terminal signal sequence for plastid import. A cDNA fragment encoding the processed form of the enzyme was expressed in Escherichia coli. The resulting protein was purified to electrophoretic homogeneity. The enzyme requires Mn(2+) and reduced thioredoxin (TRX) for activity. Spinach (Spinacia oleracea) TRX f has an apparent dissociation constant for the enzyme of about 0.2 microM. The corresponding constant for TRX m is orders of magnitude higher. In the absence of TRX, dithiothreitol partially activates the enzyme. Upon alkylation of the enzyme with iodoacetamide, the dependence on a reducing agent is lost. These results indicate that the first enzyme in the shikimate pathway of Arabidopsis appears to be regulated by the ferredoxin/TRX redox control of the chloroplast.  (+info)

3-Deoxy-D-arabinoheptulosonate 7-phosphate (DAHP) synthase (EC 2.5.1.54) is the first enzyme in a series of metabolic reactions known as the shikimate pathway, which is responsible for the biosynthesis of the amino acids phenylalanine, tyrosine, and tryptophan. Since it is the first enzyme in the shikimate pathway, it controls the amount of carbon entering the pathway. Enzyme inhibition is the primary method of regulating the amount of carbon entering the pathway. Forms of this enzyme differ between organisms, but can be considered DAHP synthase based upon the reaction that is catalyzed by this enzyme. In enzymology, a DAHP synthase (EC 2.5.1.54) is an enzyme that catalyzes the chemical reaction phosphoenolpyruvate + D-erythrose 4-phosphate + H2O ⇌ {\displaystyle \rightleftharpoons } 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate The three substrates of this enzyme are phosphoenolpyruvate, D-erythrose 4-phosphate, and H2O, whereas its two products are ...
1OFB: Substrate and Metal Complexes of 3-Deoxy-D-Arabino-Heptulosonate-7-Phosphate Synthase from Saccharomyces Cerevisiae Provide New Insights Into the Catalytic Mechanism
Researches at Prof. Gad Galili s lab elicited a significant increase in the direct products of the shikimate pathway and in the aromatic amino acid Phenylalanine. A central regulator in the shikimate pathway is the first committed enzyme of the pathway; 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DAHPS). The bacterial DAHPS is feedback inhibited by a separate amino acid. At the core of this technology is the dominant isoform that is the AroG gene which is under the regulation of Phenylalanine and responsible for 80% of the total DAHPS activity.. By expressing a mutant bacterial AroG gene encoding a feedback insensitive DAHPS in transgenic Arabidopsis plants, researchers achieved increased levels of the shikimate direct metabolites, products and aromatic amino acids. Detailed analysis revealed that while no metabolite exhibited decreased levels in the transgenic plants, the levels of shikimate intermediate metabolites, phenylalanine, tryptophan, and a verity of secondary metabolites ...
1ZCO: Substrate Ambiguity and Crystal Structure of Pyrococcus furiosus 3-Deoxy-d-arabino-heptulosonate-7-phosphate Synthase: An Ancestral 3-Deoxyald-2-ulosonate-phosphate Synthase?(,)
Erythrose 4-phosphate is a phosphate of the simple sugar erythrose. It is an intermediate in the pentose phosphate pathway and the Calvin cycle. In addition, it serves as a precursor in the biosynthesis of the aromatic amino acids tyrosine, phenylalanine, and tryptophan. It is used in the first step of the shikimate pathway. At this stage, phosphoenolpyruvate and erythrose-4-phosphate react to form 3-deoxy-D-arabinoheptulosonate-7-phosphate (DAHP), in a reaction catalyzed by the enzyme DAHP synthase. It also used in 3-hydroxy-1-aminoacetone phosphate biosynthesis, which is a precursor of vitamin B6 in DXP-dependent pathway. Erythrose-4-phosphate dehydrogenase is used to produce erythronate-4-phosphate ...
ID A0A1V1RDQ8_9ACTN Unreviewed; 293 AA. AC A0A1V1RDQ8; DT 07-JUN-2017, integrated into UniProtKB/TrEMBL. DT 07-JUN-2017, sequence version 1. DT 31-JUL-2019, entry version 7. DE SubName: Full=3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase {ECO:0000313,EMBL:GAV30768.1}; GN Name=aroA {ECO:0000313,EMBL:GAV30768.1}; GN ORFNames=emb_1d0058 {ECO:0000313,EMBL:GAV30768.1}; OS Coriobacteriaceae bacterium EMTCatB1. OC Bacteria; Actinobacteria; Coriobacteriia; Coriobacteriales; OC Coriobacteriaceae. OX NCBI_TaxID=1927122 {ECO:0000313,EMBL:GAV30768.1}; RN [1] {ECO:0000313,EMBL:GAV30768.1} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=EMTCatB1 {ECO:0000313,EMBL:GAV30768.1}; RA Kobayashi H., Fu Q., Maeda H., Sato K.; RT "Draft genome sequence of a novel Coriobacteriaceae sp. strain RT EMTCatB1 reconstructed from the metagenome of a thermophilic RT electromethanogenic biocathode."; RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases. CC -!- CAUTION: The sequence shown here is ...
Amino Acids and Derivatives,Aromatic amino acids and derivatives,Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate),3-dehydroquinate synthase (EC 4.2.3.4 ...
harga kat Msia RM2000 tapi kat sana dia boleh dapat semurah RM1,270.dan pulak kawan kpd kawan ummi tu nak order secepat mungkin.so ummi ada sehari je nak decide.yang lawaknya, ummi memang tak sevey apa barang baby pun lg.nak2 pulak breastpump.yg ummi tau breastpump memang mahal.so teruslah menggoogle dan tanya2 pendapat orang.kira Medela FS ni yg plg recommended cuma mahal melampau.so lepas dah diskas2 ngan walid, kami decide to just grab this offer.mmg bargain la kan?ada la ummi terbaca2 what if nanti susu takde or etc.breastpump dah beli mahal2 huhu.tapi ada this forum kata kalau kita dah plan nak bf baby kita sejak pregnant and always think +ve bout it, inshaallah susu tu akan ada.ummi harap begitulah, boleh bf my lil miracle selama yg ummi mampu.amiin.apapun rezeki masing2 kan...ummi redha:) oh btw physically, my nipples dah bertukar wajah.haha.sekarang aerola(yg kaler itam keliling breast) dah makin besar diameter ...
hempp.. rasanya Aida pon dah tahu citer pasal kite kan? Julie lagi sedih.. at least Aida ada banyak susu.. boleh dipam.. Julie pon ceasar jugak mcm Aida.. & nurse2 kat hospital tu tak spend banyak masa utk ajar Julie breastfeedkan baby.. maybe dia ingat kita dah reti.. memula dlm kepala pk anak2 ni mmg akan minum susu dari puting ibunya no matter what.. tapi sebenarnya tak.. masa lepas bersalin, rupanya si hubby dah benarkan nurse bagi susu botol kat Aisya sbb Aisya dah lapar.. sedangkan Julie still tak keluar dari OT.. patutla dia tanak isap puting walaupon dipujuk.. lepas balik dari hospital, sampai je rumah, hati mula runsing.. Julie dah takde mak nak tunjuk macamana nak breastfeed.. mak mertua pon tak datang lagi time tu.. takut sangat baby terjaga dari tidur & minta susu.. at last dia bangun jugak & menangis kelaparan.. yang ada hanyalah Julie & hubby.. bila disua puting dia tak reti nak isap.. nak pegang baby pon belum pandai.. sampaila sejam dia nangis.. lastly Julie give up & minta hubby ...
Makan lah kitorang 4 beranak tanpa papa.. snap gambar ni send kat Pak Radie hehehe.. Eish tengok Leecha tu sekarang dia dah jadi minah kuat membebel.. bising betol mulut dia.. Amaani pulak dah jadi pendiam sikit.. sebab Aleesya dah overshadow dia sekarang.. sentap je Amaani sebab Aleesya suka jealous dengan dia.. tapi kalau baik dia memang boleh ngam berdua tu.. Kasih pulak dah besar.. so dia suka menyakat.. dia pun suka gaduh dengan Amaani.. nanti kalau gaduh dengan Amaani dia saja buat baik dengan Aleesya.. so Amaani terus takde gang.. hahaha kesian betul Amaani jadi mangsa buli.. Tapi kalau dia buat hal boleh tahan gak.. berebut phone or ipad ke.. dia sakit hati memang dia campak je kat Aleesya pastu dia cakap "Amaani tak suka Aleesya!!! Nah lah".. Pastu Aleesya pandang dia pakai ekor mata jeeee.. Aleesya kadang tak suka menjawab tapi dia suka tengok pakai ekor mata sambil jeling.. tapi dia memang confirm menang lah.. Amaani berlalu pergi sambil melalak.. hahahah ...
hi bro semua... yg butuh bungkus jok mobil nya bisa kontak2 k gw nih.... nih daftar harga nya : bahan semi kulit : merk MBtech Camaro untuk mobil 2
Baby ni anak sedara hubby. Anak adik perempuan hubby yg nombor 2. Umo 10 bulan. Sgt comel ok. Jenis x byk ragam, senang jaga. Sblm ni sy dah pernah wat entry buh gambar baby ni jugak.. ...
Sudah beberapa hari nak berkongsi hal berkenaan sakit gigi nih tapi bila dah sakit gigi memang mood ke laut ler nak berkongsi, yer dok? Siapa pernah sakit gigi? semua kan... termasuk kanak-kanak, dewasa dan warga emas sekali pun.. Aishhhh, kalau diingat ingatkan, sakit gigi dan sakit bersalin, memang sakit lagi bersalin cuma sekejap. Tapi kalau…
akhirnye..dia dah mula drive keta..mcm2 dibuatnye..tarik signal kanan kiri..tekan hon..tekan pemanas tingkap..tekan lampu kecemasan..on off lampu besar..adoiiyaa....mana abah ko ni..lambatnyee..ummi dah pening dgn ko ni ...
teringat kat anak saya ..bila tengok anak azni..memang macam nilah .. masa umur dia 3 tahun start jadi ..sampai umur 6 tahun ...memang kesian sangat ..garu sampai berdarah.. macam2 ubat dah bagi ,... pegi pakar kulit ..beribu2 dah abis duit.. kulit akan baik untuk sementara waktu jer ,,then akan jadi balik ... last2 saya guna DRII keluaran Dr Rozmey ... dua kotak minum ubat tu .. then kulit dia jadi bersih n luka2 jadi kering... syukur sangat .. sekarang dah sekolah tahun 2 nak masuk tahun 3.. kulit dah ok.. dulu hari2 saya nangis tengok dia menggaru ..time mandi menangis ..sebab pedih .. now dah ok.. (Wahida - SR) ...
hai tengah buat apa tu ,_, dah makan malam kew? kalau sudah alhamdulillah......gendang gendut tali kecapi, dah kenyang perut pon happy...hehehe.. cewahh layan pantun jap....hehehe..masak apa tadi..ingat..jangan makan berat2 waktu malam ceng gini, sebab nanti dah nak tido kan, lambat pula nak proses nanti..betul tak ?? Entri malam ni just nak share something dengan u all. Pasai BARAH PAYU DARA ..Umumnya semua orang tahu dan pernah membacanya dimana-mana sahaja dalam majalah, tengok kat media elektronik dan sebagainya.. Saya hanya ingin mengingatkan u all dan juga kepada diri sendiri supaya sentiasa lebih peka mengenai penyakit ini...ok jom layannnnn ...
Now dah start kerja, Nia tinggal dengan my sister kat area Balakong. Memang mencabar lah sebab kul6 pagi Nia dah kene ikut my hasben keluar sekali pergi kerja. Hurmm before kul6 jugak lah budak kecik ni dah pandai bangun. Mata bulat senyum memanjang. Nia memang......excited!! Alhamdulillah. I selalu doa agar Nia happy kat rumah sendiri and rumah orang. Tak mau Nia sedih-sedih. Kalau i balik dari sekolah ambil Nia, selalu Nia tengah tidur or tengah susu. Pastu sengeh-sengeh bila nampak i. Happy letewww. ...
oo. nice info. rupanya ada kategori baby SGA eyh. Aritu anak buah lahir kecik jgk. 2.5kg. tu pon induce. mmg sbb faktor kecil.before tu si mak warded dalam 2 minggu dah. then sampai due date terus induce. Bahaya jgk rupanya baby SGA ni. Tapi alhamdulillah baby skrg dah 3bln. sihat. tapi berat xnaik sangat.. ...
Rindu pantai? - dh agak lama MZ tak balik kg especially bersama mr hubby..last sekali balik, MZ balik dgn my boys jer (my nephew& niece tlg drive my car) so last few weeks... ...
BERAT BADAN 45 KG... siyes, b4 ni nk cecah 40 kg pun susah... sejak dua mnjak org dok kata badan dah berisi, gemuk, saya happy sebab memang misi dan matlamat saya untuk menambah berat badan.. last week timbang berat badan.. and SUNGGUH TAK PERCAYA BERAT BADAN SUDAH 45 KG..! 45 KG tu wey..ingt senang ke nak jadi 45 KG?? sekarang BMI dah jadi normal... first time BMI normal.. sejak dilahirkan, BMI mmg salu xnormal..UNDERWEIGHT! but now saya bangga saya sudah tergolong dalam geng NORMAL! ...
Betul ke boleh buat bisnes dengan tmodal kecik?rm80 sahaja?Pada awalnya saya juga tidak percaya..tetapi sekarang saya dah terjebak. Jom rebut peluang bisnes online modal rm80.. Pernah tak anda berfikir nak tambah pendapatan atau nak jana sideincome dengan bisnes? Tentu ada terlintas nak buat…. Read more →. ...
7 Mei 2013, genaplah umur saya 27 tahun. Makin tua dah upanya.... Tahun ni sudah berdua...Terima kasih buat Incik Suami untuk pemberian di atas ...
Looking for ARO Retaining Ring (42CJ31)? Graingers got your back. Price:$9.65. Easy ordering & convenient delivery. Log-in or register for your pricing.
Enzymes are biological catalysts that are essential to life: they are largely very efficient and exquisitely specialised to their substrate. They are, however, predicted to have evolved from simple promiscuous catalysts (able to catalyse multiple reactions on multiple substrates). The goal of this thesis was to explore the multiple models of evolution with three model enzymes. In a complementary model for enzyme evolution, it is hypothesised that some ancient enzymes may have exhibited higher catalytic rates than their extant descendants. This model was investigated through the reconstruction of core bacterial enzymes AroA (aromatic amino acid biosynthesis) and MurA (peptidoglycan biosynthesis), from the common ancestor of modern Streptoccocci species. The ~300 million year old ancestral enzyme conformed to the model, with 20-fold higher activity than MurA enzymes in modern Streptococci. Several models for enzyme evolution, both primordial and contemporary, require a multifunctional precursor ...
Cubist Pharmaceuticals (USA) (now Merck) was conducting research on KD08 phosphate synthase inhibitors which may have potential as antibacterial agents. KD08
Minta maaf la. Baru nak update. Busy dan malas.Hehehe.Entry sebelum ni ada cakap kan saya dan husband nak pi broga hill. Akhirnya cita-cita itu menjadi. Sebelum ni hangat-hangat tahi ayam jer. Tak pergi-pergi. Lepas jer saya tulis entry tu balek terus husband ajak pi. Tanye dia baca entry saya ke. Dia kata tak. Kan memang dah plan awal-awal nak pi. Lagi pun cuti rayakan. Tapi kami pi ahad lepas. Sabtu tido umah mertua. Pagi-pagi kol 430 dah bangun. Semangat sungguh. Kol 5 dah gerak. Pagi-pagi buta dengan jalan lengang. Kami ikut jalan kajang menghala ke semenyih. Minta tolong GPS tunjuk jalan/ Alhamdulillah tak sesat. Setengah jam jugak la sampai dari serdang tu. Sampai terus berhenti kat masjid solat subuh. Memang sebelum nak sampai ke broga tu ade masjid. Lupa plak nama masjid tu ape. Lepas solat teruskan perjalanan. Nanti jumpa nottingham university. Memang dah dekat la tu. Sampai-sampai cari parking. Sebelum ni saya dah pernah pergi dah sini bersama dengan budak-budak kelas cs110. Rindu la ...
Bila Sofie nak pergi pagi tadi, Sofie cepat je meluru pada abi sofie. Sofie menganggap dirinya akan pergi berjalan-jalan. Saya dah pakaikan Sofie baju cantik. Seluar jeans dan blouse warna pink. Comel sangat Sofie. Saya tak dapat kawal air mata saya. Abi sofie kata, "dah la...jgn nangis depan Sofie". Saya tahu, abi Sofie pun sebenarnya sedih. Orang lelaki,kan. Boleh je kawal perasaan. Kita ni ibu, pasti kalah dengan perasaan sendiri. Saya menangis akhirnya. Sofie salam dan mencium tangan saya. Saya cium pipi Sofie. Saya cium tangan suami. Dan kereta suami saya terus meluncur laju, meninggalkan saya dalam esakan. Sungguh! Saya tak boleh tahan perasaan ...
Tunggu ayah mai amik kat umah asal nurse kat klinik desa tuh tak bagi ibu bawa keta dah Sejak kejadian minggu lepas sakiti perut sambil bawa keta demi takut yer beranak dalam keta. Hmmmm lepas g klinik pergi beli makan asal mmg ibu dah tak masak dah. Tak larat sgt dah Ag pon Faris dah merungut lapo sgt. Saja amik gambo Faris makan dengan laponya. Semangat gila. Lauk yang dibeli pons dia yang pilih sendiri. Ikan keli goreng ...
Tunggu punya tunggu, sampai sudah giliran saya.. Macam biasa, doc TVS terlebih dahulu.. Kali ni bukan 14 telur tapi lebih daripada 20!! Bila dah terlalu banyak, maka telur2 terpaksa la berebut sumber dan space dalam ovary.. Maka size yg terhasil rata2 adalah 10-11mm... Sepatutnya dah 16mm doc kata.. Jadi saya punya progress dah delay 3 hari.. Dinding rahim (endometrium lining) pulak Alhamdulilah dah 7.0mm ...
Azizi lebih bijak sekarang, panjang akal..... pantang nampak nenek pakai tudung, dah perasan nenek nak bawa jalan-jalan. Yelah, manja sangat dengan nenek, almaklum duduk dengan nenek dari lahir sampai sekarang ...
Sebab satu hidangan bancuhan Cinch Shake Shaklee membekalkan kepada kita 24 gram protein, 24 jenis vitamin dan mineral serta 6 gram serat. Satu hidangan tu dah cukup lengkap untuk you rasa kenyang dan perut akan rasa penuh je. Tak perlu dah nak makan makanan-makanan lain yang lebih tinggi nilai kalorinya. Dah tak perlu nak kunyah-kunyah something. Bila dah kenyang, jadi nafsu makan yang meluap-luap tu pun dapat dikurangkan betul tak ...
Sebelum try herbal blend dia dah jumpa pakar kulit dah tapi ubat tu tak okay kat muka dia..so biras saya letak herbal blend and cuci dengan diluted basic H. alhamdulillah dalam masa 10 hari dah okay dahhh ...
Yezza.. bulan ni memang OFFER BEST ! Mana taknya Offer untuk Shaklee VIVIX ! Pasti ramai yang dah ternanti-nanti, tertunggu-tunggu bilalah agaknya Shaklee VIVIX ni nak offer lagi kan? Haa.. tu lar.. masa tengah offer takmo beli.. Dah abes offer tanya lak.. hihi.. Takpe, takpe.. ni offer dah datang lagi, jangan tunggu lama-lama, silalah rembat yea.. HARGA OFFER memang berbaloi.. ...
Dah mmg kerja kau mesyuarat, hadir ajelah. Nanti kena surat tunjuk sebab pulak. Macam kau chatting tu, tu pun dikira mesyuarat juga. Mesyuarat dua orang ahli aje iaitu Pengerusi dan 1 org JK. Kalau dah selalu mesyuarat chatting, mesyuarat lain tu dah jadi macam alah bisa tegal biasa aje ...
seronok dapat jumpe kawan-kawan lame..dapat update2 cite terbaru lagi sensasi..cewaah..hehe..almaklumlah, lame dah tinggalkan alam sekolah rendah.masing-masing sekarang dah besar,kalau tak dulu kecik2 pakai tudung senget2 laa..terberak dalam suar la...tu bukan saye eh..hihi, hai macam2 lah..serius, saya rindu zaman sekolah rendah dulu.masa sekolah rendah, saya betul2 rasa keseronokkannya..hari2 nak ke sekolah sebab nak jumpa kawan2..tanak ponteng2.huhu tapi budak sekarang..haishh..baru sakit sikit dah tanak g sekolah..okeh lupekan, meh tengok pelamin pengantin yang cantik tu..purple you..macam tahu2 je ai nak buat pelamin macam ni nanti..huhu..cantiknyer ...
Dan, Boy sekarang dah boleh di harap. Basuh baju sekolah sendiri. Basuh kasut sekolah sendiri hatta gosok baju pon sendiri. Kalau sebelum ni semua Mak yang tolong buatkan, so now harus berdikari. Tapi sebenarnya, kami adik beradik kecuali Boy memang dah di dedahkan untuk berdikari sejak masuk sekolah darjah 1 lagi. Cuma Boy agak lewat. So aku tak ada masalah dengan adik-adik yang lain. kecuali Boy. Mungkin sebab dia anak bongsu, so semua benda Mak buatkan. Tapi sekarang dia dah berdkari. Yes! Berjaya train dia ...
thanks a lot dear..sbb dah bg banyak pandangan sehingga i selamat lahirkan dihospital..klu tak kat umah or dah kat keta..dah kot..hehe.. ...
Sebenarnya dah berbulan register Chilindo ni tapi barang yang berkenan di hati bila bid tak pernah dapat harga yang nak atau memang tak berkenan barang yang ditengok/dicari. Ada je orang tanya dah macamana nak beli/bid harga barang kat Chilindo nih ...
Assalamualaikum alls... dah lama KakNgah tak menjenguk blog nih, hampir 4 bulan lamanya... Agak sibuk dengan business baru yang dijalankan namun Shaklee tetap di hati, masih setia. Dah hampir 4 bulan juga KakNgah mula berniaga nasi lemak, alhamdulillah... jualan laris, rezeki dariNYA. Semua idea adik KakNgah, KakNgah ni pula memang tak pandai masak, lagi-lagi…
hujung september br stat kumpul stok ebm.time umur danial 2bln.lg sebulan nk stat keje,br terhegeh² nk kumpul stok.org lain dlm pantang dah gigih kumpul stok dah.haha ...
Yay! Sekarang dah ade dah buku wedding planner ni. So tiap benda tiap perkara i akan catat dalam ni. So tak ade la nanti miss out ke ape ke. Then boleh buat reference untuk orang lain pulak nanti. ^__^ Yeap! I akan plan sendiri wedding i. Jimat cost ...
padahal org lain dah bertimbun dpt award..xkecoh pon..sbb br sket la kita nak kecoh kan..yg dah byk,bnda 2 da biase...hehehe...pape hal pon, tQ to my dear tik coz gave me this award..syg u ketat-ketat dear ...
eeh tetibe tersampai kat Bukit Tinggi plak eh? memang pon dah plan nak pergi sini lepas event tu.. sebelum ni memang mengidam sangat lah kan nak pergi Bukit Tinggi.. terpedaya dengan gambar2 orang... cantek gile.. ala2 perancis gituuu.. tapi cukup SEKALI ni jek!!! tak mahu pergi lagi... biase2 jek rupenya.. barang2 pon mahal gilo.. saket kaki jek jalan sini..just cantek untuk bergambar jek kot.. nak naek ke sini pon tak best..jalan die jalan kecik..hutan memanjang..ak dah pikir sesat ke hape ni..seb baek betul memang jalan ke bukit tinggi.. pening kepala jangan cakap ahh kan..sumpah pening! ak buat2 sehat jek depan hanis..supaya die tak give up memanjat bukit.. muahahhaaa!!! eh tapi kalau korang nak pergi, silakan lah pergi..harap2 tak menyesal macam ak.. ...
Jangan tangguh-tangguh lagi. Mulakan diet anda pada bulan puasa ini untuk nampak lebih menarik semasa Syawal nanti! Kenapa saya sarankan anda gunakan produk ini untuk mengatasi masalah berat badan anda? 1. Saya sendiri dah guna dan dapat turunkan 21kg! 2. Saya sendiri dah buat ujian darah dan semuanya ok termasuklah buah pinggang! 3. Saya sendiri…
JP Morgan reiterated its Neutral rating on Aeropostale (NYSE: ARO) and reduced its price target from $14.00 to $13.00. JP Morgan commented, [A]lthough ARO ...
I nampak ada entry pasal Gery tapi tak jumpa. Kesiannya.. I can feel you. Ive experience before. Snowy and I been lived together for a year. I jaga dia dari kecil baru pandai berjalan. Dah macam anak sendiri and paling sedih dia mati sebab kena langgar. Pity him.. Pasti sakit.. :( Im sure Gery happy dengan your family walaupun sekejap. Tuhan nak dia bahagia sebelum dia mati sebab tu di pertemukan dengan anak you.. ReplyDelete ...
Ok, this is what i m doing for my little boy Adam Amsyar.. Menabung for his education.. I nak ajar dia menabung dari dia kecik.. Its not easy nak disiplinkan budak.. Kena train dia from small.. Then they will use to it.. InsyaAllah.. And now adam dah ade RM20.. Hihihi.. Ok meh nak cite camana i buat :) ...
Fuhh settle satu bab.. tapi yg menunggu masih ada lagi few things.. but now time to rest kejap.. lgpun mau dengan kerja nih tak pernahnya habis.. so yeah take it easy nooo.. wow, cepat jer kan masa berlalu... dah nak habis penggal persekolahan 2016.. (ayat standard cikgu2 menjelang hujung tahun ...
seawal jam 7 pagi tadi, we all dah bertolak balik ke KB, dan jam 8.30 pagi sudah sampai ke SKKK 3 untuk melunasi kelas tambahan Atiqah ...
live the jobless life to the fullest! berdebar jugak nak tunggu kerja dan takut akan dicampak ke mana laaaahhh nanti dengan perut memboyot ni. boyot lah sangat. sumorang cakap perut kecik taknampak pon mcm pregnant. dah boncit sangat okaiiii. haha ...
live the jobless life to the fullest! berdebar jugak nak tunggu kerja dan takut akan dicampak ke mana laaaahhh nanti dengan perut memboyot ni. boyot lah sangat. sumorang cakap perut kecik taknampak pon mcm pregnant. dah boncit sangat okaiiii. haha ...
Looking for ARO Manual AirControl,3-Way,2-Position,5/32 (4LB53)? Graingers got your back. Price:$72.75. Easy ordering & convenient delivery. Log-in or register for your pricing.
Živjo sem novi lastnik aro-ta letnik 88 1,4 bencin. Kako(v kater polžaj ročko) se vklopi 4 pogon ,reduktor,in kaj se vklaplja na kolesih.? Je pa to aro 10. ...
Un pasionat de design a realizat un concept interesant de ARO. Chiar daca aceasta companie auto romaneasca nu mai exista de ani buni, fiind adusa la faliment de guvernul Nastase, pasionati si iubitori
Todas medidas e marcas de pneus nacionais e importados para moto aro 16. Enviamos para todo Brasil, consulte! GUAPOR PNEUS LONDRINA (43) 3032-1921.
Todas medidas e marcas de pneus nacionais e importados para moto aro 17. Enviamos para todo Brasil, consulte! GUAPOR PNEUS LONDRINA (43) 3032-1921.
Aroh And Veena BIG FIGHT; Heres What Happened | Bigg Boss Marathi 2 Latest Update

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The present disclosure relates to engineered microorganisms that produce amino acids and amino acid intermediates. In particular, the disclosure relates to recombinant nucleic acids encoding operons that increase production of aromatic amino acids and the aromatic amino acid intermediate shikimate; microorganisms with increased production of aromatic amino acids and the aromatic amino acid intermediate shikimate; and methods related to the production of aromatic amino acids, the aromatic amino acid intermediate shikimate, and commodity chemicals derived therefrom.
The objective of the present work consisted on determining the effects of PTS and Pyk isozymes inactivation on cell physiology, metabolic flux distribution and PEP availability for aromatics biosynthesis. The inactivation of PTS in E. coli abolishes PEP-dependent glucose transport; therefore PYR production from PEP is dependent only on Pyk isozyme activities. In this study, by inactivating each Pyk isozyme in a PTS- glc+ background, strains were generated where the PEP to PYR reaction was dependent only on PykA or PykF activity. These strains were characterized by flux analysis, thus providing the first quantitative description of the metabolic consequences of the sequential elimination of activities catalyzing the PEP to PYR reaction.. The inactivation of PTS in E. coli causes a strong reduction in qGlc and μ, therefore, such mutant strains display a PTS- glc- phenotype. To improve qGlc and μ, strain VH33 has a chromosomal modification that increases its capacity for non PTS-dependent glucose ...
Today baby dah 9 weeks. Erin mmg ade appointment kat Alpha Fertility. Mesti korg pening kan kenapa erin pergi 2 hospital. hahaha..Sebenarnya kalau ikutkan kami dah tak nak pergi ke Alpha Fertility dah. Tujuan asal mmg nak buat fertility treatment aje kat sini. Ingatkan, hari tu dah last jumpa doc dah. Tapi disebabkan ada spotting tu, hubby erin pon risau jugak. dia kata, baik kita jumpa je doc kan. At least kita tau keadaan baby kan. Erin bgtau la kat doc yang erin ade brownish spotting sikit. Doc kata, kalau spotting kaler coklat tu biasanya okay aje. Jangan spotting yang kaler fresh blood tu. yang merah tu bahaya. Pastu doc tanya erin ada sakit ke tak.. erin kata la tak de sakit langsung. Pastu doc terus scan. Kali ni doc dah tak buat TVS lagi. lega jugak la kan. Tak payah scan ikut bawah. doc kata baby dah cukup besar untuk nampak kalau scan kat perut. Inilah hasilnya... ...
Saya tak kira, saya tetap salahkan pemeriksa kertas, mesti dia gaduh dengan bini dia.. Saya pun buatlah semak semula, bayar rm50 kalau tak silap.. dalam 2/3minggu lepas tu, lecturer panggil pegi bilik dia.. Dia cakap, " Keputusan semak semula awak dah keluar ni..ish.. saya nak cakap pun salah, tak cakap pun salah.. Sape suruh awak rajin-rajin pegi cek balik.. Pointer awak dari 3.77 dah turun jadi 3.5, awak tau tak!! Ni lah orang panggil tamak! Saya kesal benar dengan awak".. Dengan muka bengis dia cakap.. Saya ni dah lah pantang kena herdik.. Terus nangis depan lecturer tu.. Mind u, lecturer tu lelaki ok.. saya tak segan silu dah nangis teresak-esak depan dia.. Masa tu dia tak bagi lagi slip periksa tu kat saya.. "Saya pun tak tau la nak cakap apa dengan student yang tak reti-reti bahasa ni.. Dulu saya dah cakap, jangan semak! Jangan semak! Ni pointer dah turun.. awak nak amek course apa nanti? 3.5 je.. ish! Nah! Amek ni! Tengok sendiri!" Time tu saya masuk bilik lecturer tu dengan my bestfren, ...
abid masih demam tapi kalini dia dah melepek, tak nak join dah kakaks bermain..makan pun tak nak susu pun tak minum..saya dah risau dah ni tapi masa tu serius saya tak terfikir langsung pasal denggi walaupun kawasan rumah kami ni black area..saya ingatkan demam biasa..petang balik keje tu tengok abid memang terbaring melepek je..lepas maghrib kami terus ke kk presint 9..check darah platlet 120++..doc kata denggi ni tapi untuk confirmkan darah abid tadi di check sekali lagi..dalam setengah jam menunggu doc panggil masuk balik dah sahkan abid POSITIF DENGGI..nak terduduk rasanya bila dengar doc cakap macamtu..ye lah dah la abid ada masalah jantung ni denggi pula, doube triple risau saya..bila tengok keadaan abid macam tu doc cepat-cepat call hospital dan abid kena dihantar dengan ambulan..ayahnya yang bawa abid dengan ambulan sementara saya dan ida (my twin) balik rumah hantar maryam dan kemaskan barang-barang dan keperluan abid dan ayahnya..dalam pukul 1.00 pagi saya dan ida sampai semula di ...
Assalamualaikum dan selamat sejahtera... arini skola amik cuti so bleh la berjimba2 kat sini jap.. lama da wat nih... dah jadi baja dah pon.. sori ehh.... hehe... sebelum terlupa, nak mintak maap yer sape2 yg tanya mana pegi sbox hanamemories tuh sebenonyer dah lama kene tendang! jatuh talak 3! malas nak rujuk dah..haha.. bg mereka2 yang biasa komen kat situ dan takleh nak regis sbg blogger, bleh ajukan apa2 persoalan kat emel yg ader kat side bar tuh eh... sori selori sekontena.. sebut psl kontena, arini barulah sampai brg2 kontena tuh ke kuantan... nak bukak and kemas balik tuh yg malessssss bangettt....... ada sape nak tulunnn?? huh! but siputih masih lg dalam proses... yg tuh lambat sket la... tp alhamdulillah rezeki sihitam dah tiba dulu.. :D .. k jom tengok resepi... resepi lama boleh tengok SINI or SINI cuma aku edit sket ...
adalah normal bila bayi selsema, akan datang pakej batuk dan demam sekali. kalau yang kuat sikit antibodinya, tak demam lah.. just batuk dan selsema.. itupun dah cukup buat ibu2 rasa gundah gulana bila baby kita tercungap2 nak bernafas, mukus tak berhenti mengalir (lambat lap dah jilat2 dah..hihi), dan batuk kong kong siang malam.. bila dah macam gitu keadaan baby, mestilah baby susah nak tidur.. bila baby tak lena tidur, kita pun tak lena tidur juga, betul tak? . baby Nailah saya (anak ke 3 saya), baru2 ni di serang selsema yang agak lama. sebelum ni pernah jugak selsema.. tapi sehari 2 dah sembuh sepenuhn ...
adalah normal bila bayi selsema, akan datang pakej batuk dan demam sekali. kalau yang kuat sikit antibodinya, tak demam lah.. just batuk dan selsema.. itupun dah cukup buat ibu2 rasa gundah gulana bila baby kita tercungap2 nak bernafas, mukus tak berhenti mengalir (lambat lap dah jilat2 dah..hihi), dan batuk kong kong siang malam.. bila dah macam gitu keadaan baby, mestilah baby susah nak tidur.. bila baby tak lena tidur, kita pun tak lena tidur juga, betul tak? . baby Nailah saya (anak ke 3 saya), baru2 ni di serang selsema yang agak lama. sebelum ni pernah jugak selsema.. tapi sehari 2 dah sembuh sepenuhn ...
hari khamis mmg totally havoc..terpacak di bawah kepanasan matari kat area parking inventQjaya dari pukul 7.30 pagi sampai pukul empat petang..pagi bermandi hujan sebab hujan turun renyai-renyai..then start dah dekat tengahari, matahari memang terik gila..tuhan je tahu macam mana rasanya berjemur bawah matahari kat CYBERJAYA..kita orang guna sistem nombor untuk pick up parents..nak hantar mereka ke cyberia..dah la parents ada dekat seratus lebih..saya memang dah tertekan gila menguruskan mak bapak yang tak tahu bahasa, dah la panas terik, mak bapak tu tak paham bahasa nak ikut giliran, contoh buruk bagi anak-anak mereka..siap ada 3 parents yang attack saya and jason serentak satu masa tu..bahasa pulak buruk..tapi kita orang sabar aje la..tapi alhamdullilah la, semuanya okay aje lepas saya bertegas dengan mak bapak..tapi memang kena sun burnt ah..saya ni dah la tak cerah mana..jadi makin gelap sekarang..haha..jumaat, sabtu, ahad, semuanya okay, agak sibuk jugak, driver bas banyak yang meragam, ...
Semalam, buat operasi bersih rumah. Boleh tahan juga, lantai semua dah lap. Mainan budak semua dah transfer ke bilik baru dia orang. Bilik tengah dijadikan bilik untuk budak-budak main dan buat homework. So sekarang, living room dah clear. Lega. Takde la kalau tetamu datang, rumah macam tongkang pecah macam dulu. Ramai yang dah balik kg. Selamat hari raya, maaf zahir dan batin pada semua yang mengenali Yati! Semoga hari raya kali ini, membawa seribu keinsafan dan pengertian pada semua! ...
Yeayy..kejap je dah hari Jumaat..Ye le sebab aku tak keje 4 hari...Ngeh3x..Isnin-Rabu ambik exam KPSL...Semalam hari untuk merehatkan otak dan hari ini dah hari Jumaat...So, esok kita enjoy...Tapikan bukannya boleh enjoy...Dah sah2 le kena mendobi baju2 yang berlambak kat dalam bakul tu, kemas rumah lagi...Wah, penatnya!!! Dahtu nak buat camne???? Dah memang rutin mingguannya gitu...Esok, Hubby kerja laks...Sudahnya melepaks aje le di rumah bersama anak2...So, korang semua apa aktiviti hujung minggu ni??? Apa lagi duk rumah kita layan TV pun jadi le... ...
im back..setelah seminggu bercuti balik kg, arini dah start keje balik..penat badan masih terasa..dlm hati rasa mau je smbung cuti tp bila fikirkan cuti pun dah xbyk maka gagahkan diri gakla keje arini..eniwey byk cerita nk cerita maklumla kata cuti seminggu kan???tp gambar2 masih dlm kamera so xleh update lagi..nti sy stori mori ek..now nk intai2 bos besar n bos kecik, nk check line sbb rasa2nya mata ni xleh tahan sgt dah..mau zzzzZZZZZzzzzzzzzzz...tadaaa.. ...
lupe nak btau...esok ciksu blk kg...cuti sempena ari deepavali...yeeehooo...xsaba sgt dah rase..lame gle dah xblk kg...insya Allah blk ni ciksu nak g cr bridal n kalo ade yg berkenan trus book sbb mase yg ade dah x byk..hope so,i will find the dais dat i like in affordable price...pray 4 me ek... ...
kemaman, cherating dan kawasana sekitarnya masa kami lalu malam tue dah tenggelam dek air, nasib baik la ada en lori didepan, so follow en lori dari belakang, dapat la kami redah air banjir tue alhamdulillah... banyak jalan dah ditutup, nasib kami baik sebab sepanjang jalan ada pihak bertanggungjawab yang menjaga jalan-jalan yang bahaya untuk dilalui so tak ada lah tenggelam and tersekat dalam air banjir. Dari jalan pantai ke jalan pelabuhan, berderet kereta bergerak menuju ke arah jalan besar by pas sebab situ je jalan yang paling tinggi dan tak tenggelam dek air untuk menuju ke kuantan. Alhamdulillah, dalam pukul 9 lebih kami selamat sampai di rumah... sentap jugalah hati sebab tengok depan rumah dah masuk air... dalam kepala me and en hubby... laptop ...
kemaman, cherating dan kawasana sekitarnya masa kami lalu malam tue dah tenggelam dek air, nasib baik la ada en lori didepan, so follow en lori dari belakang, dapat la kami redah air banjir tue alhamdulillah... banyak jalan dah ditutup, nasib kami baik sebab sepanjang jalan ada pihak bertanggungjawab yang menjaga jalan-jalan yang bahaya untuk dilalui so tak ada lah tenggelam and tersekat dalam air banjir. Dari jalan pantai ke jalan pelabuhan, berderet kereta bergerak menuju ke arah jalan besar by pas sebab situ je jalan yang paling tinggi dan tak tenggelam dek air untuk menuju ke kuantan. Alhamdulillah, dalam pukul 9 lebih kami selamat sampai di rumah... sentap jugalah hati sebab tengok depan rumah dah masuk air... dalam kepala me and en hubby... laptop ...
MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersRiboflavin, FMN, and FAD3,4-dihydroxy-2-butanone-4-phosphate synthase (TIGR00506; EC 4.1.99.12; HMM-score: 247.4) ...
upsss... ada n3 lagi?? ngeee~~~ ni la dia kalau dah malas update blog... bila sampai takat beku..., meroyan sakan la gamaknya... kali ni nak tepek cintan cintun si kakak dan si adik... si kakak ni, sejak2 ada adik... dia dah start jadik PENCINTA LELAKI = PENCIUM SI ADIK.. :) lalu dekat adik... "niya mau bobo amaa adik....".. kejap2.. "niya mauuuu yummmm adik..." jap jap... "jom niya egangggg adik..." mcm2 daaa ...
The ingredient: You only need Cili kering (if already blended dalam 2-3 sudu ikut suka la...), bawang merah, bawang putih and udang kering (dalam sesudu gitu) - All blended together. Tumis the blended ingredients with daun kari. Bila dah garing, bleh la masukkan udang. After letak garam and udang dah masak, bleh la tutup api and masukkan air limau kasturi (dalam 3-5 biji buah limau kasturi, ikut rasa yang kita suka). Gaul biar sebati. Sedap makan ngan nasik putih ...
i dah malas nak catu2 makan i lagi sbb i dah ada solution baru iaitu dengan pemakaian PREMIUM BEAUTIFUL... lagi best bila pakai set ni, u tak payah nak diet la sbb u boleh makan apa saja u nak (tp jgn l sampai semua benda u nampak, u all balun) bantu membakar kalori dalam badan, klu u all bersenam lagi cepat mmbantu... dan banyak lagi kelebihan & keistimewaannya.... ...
Sepanjang usia 7 bulan ni, Faris dah pandai duduk dengan agak stabil. Tapi still aku letak bantal-bantal keliling dia, takut tumbang. Kalu dah dapat duduk tu, makin kuat la dia main. Tapi....Faris ni belum pandai merangkak lagi, meniarap pun belum pandai bergerak ke depan, gear reverse pandai la dia ...
Pernah ada satu masa tu, bacaan gula abah di tahap maksimum..doktor paksa abah warded tapi abah taknak..dia serik...balik rumah cuba control sendiri..jaga makan.. Eina cuba bagi abah Vivix....ambil dengan konsisten selama lebih kurang 3-4 bulan…Alhamdulillah bacaan gula abah cantikkk. Itu pun dah cukup happy bila dengar. Abah pun terkejut..sejak hari tu, abah memang tak pernah tinggal Vivix sesudu kecil setiap hari. Bukan sahaja bacaan gula je cantikkk…malah darah tinggi juga dah cantikkk. ...
First mesti basuh cendawan putih ni bersih-bersih. Lepas dah basuh rebus dalam periuk sehingga cendawan putih kembang dan lembut dan air mendidih. Boleh tambah gula batu untuk rasa manis. Kadang-kadang saya rebus dengan buah lai. Kalau rebus dengan buah lai tak payah campur gula batu. Sebabnya buah lai tu dah manis ...
wa rasa sbb dia tau profile dia ada byk problem,byk kes,tu yg dia kena cepat tabur gula2 kat tanah.satu cara yg cepat,dgn ikut idea2 anwar.sume dia nak buat beb.1st dulu,idea lompat2 parti.pastu,16sept dah umum cuti penubuhan msia.skrg pun dah pandai guna perkataan rakyat.sekolah 1 aliran dia nak buat gak ...
Modificat si ranforsat sasiul, modificat suspensii, fuzete si bascule facute la comanda, inlocuit frane tambur cu discuri fata si spate si etriere Brembo de la Mercedes, montat motor Mercedes 3200 Diesel, cutie Mercedes cuplata prin cardan intermediar (construit din 2 cardane, Mercedes si Aro) cu reductor Aro, jante 17. Merge impecabil si franeaza incredibil ...
Nama nak taip panjang, I think I would name him as MS for Mr. Saifudin... Nak muntah, ur pleasure, sebab lepas ni, akan ada banyak lagi nama dia kat sini... Dia dah jadi the biggest part in my life ...
Ingredients, allergens, additives, nutrition facts, labels, origin of ingredients and information on product Gingembre moulu - ARO - 500g
... is situated in the heart of the Costa Brava has an array of hidden coves, spectacular views and crystal clear water making it the perfect destination...
Kharisma Aro is using Eventbrite to organize 3 upcoming events. Check out Kharisma Aros events, learn more, or contact this organizer.
... Informatii generale, dotari standard si optionale, afla detaliile amanuntite pentru modelul ales de tine.
Ofertas de Pneu Aro 17 225 45 incríveis, entrega rápida e garantida é no Shoptime. Aproveite para comprar no conforto da sua casa! Produtos em oferta no Shoptime, 24h com vocês.
ARO is the highest rated rafting company in two categories of the "'Adirondacks Best of 2018."  We are tops in the "Best kayaking...
Confira ➤ Kit 4 Pneus Kumho Aro 13 Solus Sense KR26 165/70R13 79T. ➽ O Preço em Promoção ou Cupom de Desconto da Oferta Podem Esgotar Rapidamente!
Com rcio de Acess rios e Pe as para Minis. Importamos directamente de Inglaterra. Pre os imbat veis, contacte-nos. Fazemos entregas em todo o pa s
P15 has a predicted Rho independent terminator at the 3' end but the function of P15 is unknown. Pseudomonas sRNA P1 ... P15 is conserved across several Pseudomonas species and is consistently located upstream of a 3-deoxy-7-phosphoheptulonate ... synthase gene. ...
DAH7-P synthase, DAHP synthase, DS-Co, DS-Mn, KDPH synthase, KDPH synthetase, deoxy-D-arabino-heptulosonate-7-phosphate ... Metal ions are required in order for DAHP synthase to catalyze reactions.[2] In DAHP synthase, it has been shown that binding ... The quaternary structure of DAHP synthase consists of two tightly bound dimers, which means that DAHP synthase is a tetramer.[5 ... To the right is an image of DAHP synthase that shows the quaternary structure of DAHP synthase. This image shows that DAHP ...
... acetolactate synthase EC 2.2.1.7: 1-deoxy-D-xylulose-5-phosphate synthase EC 2.2.1.8: fluorothreonine transaldolase EC 2.2.1.9 ... synthase EC 2.3.3.2: decylcitrate synthase EC 2.3.3.3: citrate (Re)-synthase EC 2.3.3.4: decylhomocitrate synthase EC 2.3.3.5: ... synthase EC 2.4.1.12: cellulose synthase (UDP-forming) EC 2.4.1.13: sucrose synthase EC 2.4.1.14: sucrose-phosphate synthase EC ... squalene synthase EC 2.5.1.22: spermine synthase EC 2.5.1.23: sym-norspermidine synthase EC 2.5.1.24: discadenine synthase EC ...
DAH7-P synthase, DAHP synthase, DS-Co, DS-Mn, KDPH synthase, KDPH synthetase, deoxy-D-arabino-heptulosonate-7-phosphate ... Metal ions are required in order for DAHP synthase to catalyze reactions.[2] In DAHP synthase, it has been shown that binding ... The quaternary structure of DAHP synthase consists of two tightly bound dimers, which means that DAHP synthase is a tetramer.[5 ... To the right is an image of DAHP synthase that shows the quaternary structure of DAHP synthase. This image shows that DAHP ...
Neisseria meningitidis 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (NmeDAH7PS) adopts a homotetrameric structure ... The Functional Unit of Neisseria Meningitidis 3-Deoxy-ᴅ-Arabino-Heptulosonate 7-Phosphate Synthase Is Dimeric PLoS One. 2016 ... Neisseria meningitidis 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (NmeDAH7PS) adopts a homotetrameric structure ...
... giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP). ... Chorismate synthase (aroC). This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic ... Belongs to the class-I DAHP synthase family.Curated. Phylogenomic databases. evolutionary genealogy of genes: Non-supervised ... 3. EBI-1125143,EBI-543750. GO - Molecular functioni. *identical protein binding Source: EcoCycInferred from direct assayi* ...
PABA synthase * Transaminases * Phosphotransferases * Phosphotransferases (Alcohol Group Acceptor) * shikimate kinase * 4- ... Four enzymes of the respective biosynthetic chain were demonstrated in isolated plasmodia: 3-deoxy-D-arabino-heptulosonate-7- ...
P15 has a predicted Rho independent terminator at the 3 end but the function of P15 is unknown. Pseudomonas sRNA P1 ... P15 is conserved across several Pseudomonas species and is consistently located upstream of a 3-deoxy-7-phosphoheptulonate ... synthase gene. ...
vsad1 is a previously identified allele of the transparent testa 4 gene (tt4), which encodes chalcone synthase (CHS), a key ... A chalcone synthase controls the verticillium disease resistance response in both Arabidopsis thaliana and cotton. ... Characterization and expression of chalcone synthase gene from Ginkgo biloba. Plant Science, 168, 1525-1531.CrossRefGoogle ... Enzymatic properties and mutational studies of chalcone synthase from Physcomitrella patens. International Journal of Molecular ...
egn:BMF35_a1970 CTP synthase K01937 545 102 ( -) 29 0.309 81 -, 1 fib:A6C57_11525 carbamoyl phosphate synthase small subu ... abam:B1s21122_03435 CTP synthase K01937 545 100 ( -) 29 0.326 92 -, 1 aep:AMC99_01220 CTP synthase K01937 544 100 ( -) 29 0.321 ... vmo:VMUT_0696 CTP synthase K01937 548 101 ( -) 29 0.347 75 -, 1 woc:BA177_01830 hypothetical protein 317 101 ( -) 29 0.317 82 ... hrb:Hrubri_2236 polyketide synthase 4172 115 ( -) 32 0.337 104 -, 1 orh:Ornrh_0096 putative N-acetylglucosamine kinase 287 115 ...
CHS (chalcone synthase) and CHI (chalcone isomerase) are rate-limiting enzymes and involved in male sterility when their ... An anther-specific chalcone synthase-like gene D 5 related to rice pollen development. Chin Sci Bull. 2000;45(21):1921-6.View ... LAP5 and LAP6 encode anther-specific proteins with similarity to chalcone synthase essential for pollen exine development in ... Kehrel B, Wiermann R. Immunochemical localization of phenylalanine ammonia-lyase and chalcone synthase in anthers. Planta. 1985 ...
DAH7-P synthase, DAHP synthase, DS-Co, DS-Mn, KDPH synthase, KDPH synthetase, deoxy-D-arabino-heptulosonate-7-phosphate ... Metal ions are required in order for DAHP synthase to catalyze reactions.[2] In DAHP synthase, it has been shown that binding ... The quaternary structure of DAHP synthase consists of two tightly bound dimers, which means that DAHP synthase is a tetramer.[5 ... synthase His_biosynth HMGL-like IGPS IMPDH KDGP_aldolase Lys-AminoMut_A MtrH NanE NAPRTase NeuB NMO OAM_alpha OMPdecase Orn_Arg ...
Banks IR, Specht CA, Donlin MJ, Gerik KJ, Levitz SM, Lodge JK: A chitin synthase and its regulator protein are critical for ... These components are diacylglycerol o-acyltransferase (DGAT), chitin synthase regulator 2 (CHSr), hemolysin-iii channel protein ... HSP70 and ATP synthase f0 subunit 9 (ATPS9). These genes were chosen because of their high frequency or as representatives of ... as indicated by up-regulation of DGAT and phosphatidyl synthase (PHS), which produce phospholipids. DGAT has been found in ...
Other names: G. violaceus, G. violaceus PCC 7421, Gloeobacter, Gloeobacter violaceus, Gloeobacter violaceus ATCC 29082, Gloeobacter violaceus PCC 7421, Gloeobacter violaceus str. PCC 7421, Gloeobacter violaceus strain PCC 7421, Gloeobacterales, Gloeobacterales Cavalier-Smith 2002, Gloeobacteria, Gloeobacteria Cavalier-Smith 2002 ...
Crystal Structure of the Double Complex of the Tyrosine Sensitive Dahp Synthase from Yeast rcsb ... Crystal structure of the tyrosine-regulated 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase from saccharomyces cerevisiae ...
C16 - Congenital, Hereditary, and Neonatal Diseases and Abnormalities ...
Double complex of the tyrosine sensitive dahp synthase from s. cerevisiae with co2+, pep and the e4p analogoue g3p ... Substrate and Metal Complexes of 3-Deoxy-D-Arabino-Heptulosonate-7-Phosphate Synthase from Saccharomyces Cerevisiae Provide New ...
3. Amino acid anaktsus abd tryptic fingerprints indicated that the subunits of the enzyme are very similar and possibly ... 1. 3-Deoxy-D-arabinoheptulosonate-7-phosphate synthetase(phe) from Escherichia coli K12 has been purified to near homogeneity. ... Studies on 3-Deoxy-d-arabinoheptulosonate-7-phosphate Synthetase(phe) from Escherichia coli K12. 1. Purification and Subunit ...
... synthase. cog/ cog. 3-deoxy-7-phosphoheptulonate synthase activity. go/ molecular_function. chorismate mutase activity. go/ ... POSITION A C G T 1 0.0 1.0 0.0 0.0 2 0.0 0.0 0.0 1.0 3 1.0 0.0 0.0 0.0 4 0.0 1.0 0.0 0.0 5 1.0 0.0 0.0 0.0 6 1.0 0.0 0.0 0.0 7 ... POSITION A C G T 1 0.0 1.0 0.0 0.0 2 0.0 0.0 1.0 0.0 3 1.0 0.0 0.0 0.0 4 0.0 0.0 1.0 0.0 5 0.0 1.0 0.0 0.0 6 0.0 0.0 1.0 0.0 ... 3-ketoacyl-(acyl-carrier-protein) reductase (RefSeq). 229, 356. BSU21700. ypoP. putative transcriptional regulator (MarR family ...
SPFH/Band 7 domain protein (VIMSS). 23, 256. GSU2443. GSU2443. dehydrogenase complex, E1 component, alpha subunit (VIMSS). 167 ... POSITION A C G T 1 0.8 0.0 0.2 0.0 2 0.2 0.0 0.0 0.8 3 0.0 0.8 0.0 0.2 4 1.0 0.0 0.0 0.0 5 0.2 0.0 0.8 0.0 6 0.0 1.0 0.0 0.0 7 ... POSITION A C G T 1 1.0 0.0 0.0 0.0 2 0.8 0.2 0.0 0.0 3 0.0 0.0 0.8 0.2 4 0.6 0.0 0.4 0.0 5 0.8 0.2 0.0 0.0 6 0.6 0.0 0.0 0.4 7 ... POSITION A C G T 1 0.0 0.0 1.0 0.0 2 0.0 0.8 0.2 0.0 3 0.2 0.4 0.0 0.4 4 0.8 0.0 0.0 0.2 5 0.0 0.6 0.0 0.4 6 0.0 0.8 0.2 0.0 7 ...
Substrate Ambiguity and Crystal Structure of Pyrococcus furiosus 3-Deoxy-d-arabino-heptulosonate-7-phosphate Synthase: An ... Crystal structure of pyrococcus furiosus 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase. ...
ATP synthase type III secretion protein N [EC:3.6.3.14]) but in no other rumen bacteria.. ... 2013;7:316-21.View ArticlePubMedPubMed CentralGoogle Scholar. *. Bain WE, Bezuidenhout L, Jopson NB, Pinares-Patino C, McEwan ... 1975;64:540-3.View ArticlePubMedGoogle Scholar. *. Porschen RK, Chan P. Anaerobic vibrio-like organisms cultured from blood: ... 2007;23:3251-3.View ArticlePubMedGoogle Scholar. *. R Core Team. R: a language and environment for statistical computing. ...
POSITION A C G T 1 1.0 0.0 0.0 0.0 2 0.0 0.0 1.0 0.0 3 0.0 0.5 0.5 0.0 4 0.0 0.0 1.0 0.0 5 1.0 0.0 0.0 0.0 6 1.0 0.0 0.0 0.0 7 ... POSITION A C G T 1 0.0 1.0 0.0 0.0 2 0.0 0.0 1.0 0.0 3 1.0 0.0 0.0 0.0 4 0.0 0.0 1.0 0.0 5 0.0 1.0 0.0 0.0 6 0.0 0.0 1.0 0.0 ... bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase (RefSeq). 229, 315. BSU30310. ytwF. putative sulfur ... POSITION A C G T 1 0.0 1.0 0.0 0.0 2 0.0 1.0 0.0 0.0 3 0.0 0.333333 0.0 0.666667 4 0.0 1.0 0.0 0.0 5 0.0 0.333333 0.666667 0.0 ...
pseudaminic acid synthase (TIGR03586; EC 2.5.1.97; HMM-score: 28.5) MetabolismAmino acid biosynthesisAromatic amino acid family ... Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) Chorismate mutase I (EC 5.4.99.5) / 2-keto-3- ... deoxy-D-arabino-heptulosonate-7-phosphate synthase I beta (EC 2.5.1.54) ... product: bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase. ⊟Genome View[edit source , edit]. Meta Function ...
cysteine synthase [1] (data from MRSA252). SACOL_RS02930. pyridoxal 5-phosphate synthase lyase subunit PdxS [1] (data from ... GMP synthase (glutamine-hydrolyzing) [1] (data from MRSA252). SACOL_RS02640. YbaB/EbfC family nucleoid-associated protein [1] ( ... beta-ketoacyl-[acyl-carrier-protein] synthase II [1] (data from MRSA252). SACOL_RS05135. oligoendopeptidase F [1] (data from ... 1,4-dihydroxy-2-naphthoyl-CoA synthase [1] (data from MRSA252). SACOL_RS05470. bifunctional methylenetetrahydrofolate ...
F0F1 ATP synthase subunit beta [1] (data from MRSA252). SAUSA300_1096. (carB). carbamoyl phosphate synthase large subunit [1] ( ... 3-oxoacyl-(acyl carrier protein) synthase III [1] (data from MRSA252). SAUSA300_2054. (fabZ). (3R)-hydroxymyristoyl-ACP ... translation initiation factor IF-3 [1] (data from MRSA252). SAUSA300_2506. (isaA). immunodominant antigen A [1] (data from ... glyceraldehyde-3-phosphate dehydrogenase, type I [1] (data from MRSA252). SAUSA300_1881. (gatA). aspartyl/glutamyl-tRNA ...
3 LACT02396 Cas9/Csn1 0 0 0 1/1 1/1 0 0 0 1/1 0 3 3 LCRIS_01207 cas-CT1978 0 0 0 0 0 0 0 0 0 1/1 1 1 LCRIS_01209 casE-Cse3 0 0 ... STMUK_2027 3-5 exoribonuclease from Salmonella enterica subsp. enterica serovar Typhimurium str. UK-1. Aligns to 2:178 / 189 ... EXOD_BPT4 / P04536 Exodeoxyribonuclease; 3-5 exonuclease; EC 3.1.11.1 from Enterobacteria phage T4 (Bacteriophage T4) (see 2 ... EXRBN_MYCTU / P9WJ73 3-5 exoribonuclease Rv2179c; EC 3.1.13.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) ( ...
... synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high ... concentration of tyrosine or tryptophan 2 3 4 Name Description. AROmatic amino acid requiring Comparative Info. Integrated ...
  • Structure of Aquifex aeolicus kdo8ps in complex with z-methyl-pep 2-dehydro-3-deoxyphosphooctonate aldolase. (wikipedia.org)
  • Forms of this enzyme differ between organisms, but can be considered DAHP synthase based upon the reaction that is catalyzed by this enzyme. (wikipedia.org)
  • The primary function of DAHP synthase is to catalyze the reaction of phosphoenolpyruvate and D-erythrose 4-phosphate to DAHP and phosphate . (wikipedia.org)
  • Feedback inhibition and transcriptional control are both mechanisms of regulating carbon in bacteria, but the only mechanism of regulation found in DAHP synthase found in plants is transcriptional control. (wikipedia.org)
  • In Escherichia coli , a species of bacteria, DAHP synthase is found as three isoenzymes , each of which sensitive to one of the amino acids produced in the shikimate pathway. (wikipedia.org)
  • [3] It was also determined that the enzyme is inhibited by inorganic phosphate through noncompetitive inhibition with respect to both substrates and inhibited by DAHP through competitive inhibition with respect to phosphoenolpyruvate and noncompetitive inhibition with respect to D-erythrose 4-phosphate. (wikipedia.org)
  • In plants, as the plants progressed through the growth cycle, the activity of DAHP synthase changed. (wikipedia.org)
  • Metal ions are required in order for DAHP synthase to catalyze reactions. (wikipedia.org)
  • In DAHP synthase, it has been shown that binding site contains patterns of cysteine and histidine residues bound to metal ions in a Cys-X-X-His fashion. (wikipedia.org)
  • It has been shown that, in general, DAHP synthases require a bivalent metal ion cofactor in order for the enzyme to function properly. (wikipedia.org)
  • Studies have suggested that one metal ion bonds to each monomer of DAHP synthase. (wikipedia.org)
  • Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP). (uniprot.org)
  • There are 3 DAHP synthases, AroH is feedback-inhibited by Trp. (uniprot.org)
  • The other 2 DAHP synthases are Tyr- and Phe-sensitive, respectively. (uniprot.org)
  • The three substrates of this enzyme are phosphoenolpyruvate , D-erythrose 4-phosphate , and H 2 O , whereas its two products are 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate and phosphate . (wikipedia.org)
  • [3] Studies of product inhibition have shown that phosphoenolpyruvate is the first substrate to bind to the enzyme complex, inorganic phosphate is the first product to dissociate from the enzyme complex. (wikipedia.org)
  • 3. Amino acid anaktsus abd tryptic fingerprints indicated that the subunits of the enzyme are very similar and possibly identical. (edu.au)
  • shock proteins, EC1303_c16710 and EC1303_c16770, respectively), and recE (exonuclease VIII, 5 ->3 specific dsDNA exonuclease, EC1303_c16970 ) also lie within the same prophage region. (lbl.gov)
  • Four enzymes of the respective biosynthetic chain were demonstrated in isolated plasmodia: 3-deoxy-D-arabino-heptulosonate-7-phosphate synthetase (EC 4.2.1.15), shikimate dehydrogenase (EC 1.1.1.25), shikimate kinase (EC 2.7.1.71) and pABA synthetase. (nih.gov)
  • Phosphoenolpyruvate + D-erythrose 4-phosphate + H 2 O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate. (uniprot.org)
  • P15 has a predicted Rho independent terminator at the 3' end but the function of P15 is unknown. (wikipedia.org)
  • Neisseria meningitidis 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (NmeDAH7PS) adopts a homotetrameric structure consisting of an extensive and a less extensive interface. (nih.gov)
  • Growth occurs optimally at 80°C and pH 2.5-3. (up.ac.za)
  • In this paper we present new results obtained with our previously presented high-throughput parallel SPM system [3,that showcase two key advances that are required for a successful deployment of SPM in high-throughput metrology, defect and mask inspection. (tudelft.nl)
  • [3] This method is also found in bacteria, but feedback inhibition is more prevalent. (wikipedia.org)