3,3'-DiaminobenzidineBenzidines: Very toxic industrial chemicals. They are absorbed through the skin, causing lethal blood, bladder, liver, and kidney damage and are potent, broad-spectrum carcinogens in most species.Aminobiphenyl Compounds: Biphenyl compounds substituted in any position by one or more amino groups. Permitted are any substituents except fused rings.PeroxidasesHistocytochemistry: Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.Cyanides: Inorganic salts of HYDROGEN CYANIDE containing the -CN radical. The concept also includes isocyanides. It is distinguished from NITRILES, which denotes organic compounds containing the -CN radical.Horseradish Peroxidase: An enzyme isolated from horseradish which is able to act as an antigen. It is frequently used as a histochemical tracer for light and electron microscopy. Its antigenicity has permitted its use as a combined antigen and marker in experimental immunology.Staining and Labeling: The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Organoids: An organization of cells into an organ-like structure. Organoids can be generated in culture. They are also found in certain neoplasms.Catalase: An oxidoreductase that catalyzes the conversion of HYDROGEN PEROXIDE to water and oxygen. It is present in many animal cells. A deficiency of this enzyme results in ACATALASIA.Immunoenzyme Techniques: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Pennisetum: A plant genus of the family POACEAE. The seed is one of the millets used in EDIBLE GRAIN. It contains vitexin. The common name of buffelgrass is also used for CENCHRUS.Panicum: A plant genus of the family POACEAE. The seed is one of the EDIBLE GRAINS used in millet cereals and in feed for birds and livestock (ANIMAL FEED). It contains diosgenin (SAPONINS).ArchivesBiological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Prokaryotic Initiation Factor-2: The largest of the three prokaryotic initiation factors with a molecular size of approximately 80 kD. It functions in the transcription initiation process by promoting the binding of formylmethionine-tRNA to the P-site of the 30S ribosome and by preventing the incorrect binding of elongator tRNA to the translation initiation site.Glass: Hard, amorphous, brittle, inorganic, usually transparent, polymerous silicate of basic oxides, usually potassium or sodium. It is used in the form of hard sheets, vessels, tubing, fibers, ceramics, beads, etc.Chloral Hydrate: A hypnotic and sedative used in the treatment of INSOMNIA.Plastics: Polymeric materials (usually organic) of large molecular weight which can be shaped by flow. Plastic usually refers to the final product with fillers, plasticizers, pigments, and stabilizers included (versus the resin, the homogeneous polymeric starting material). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Pharmaceutical Solutions: Homogeneous liquid preparations that contain one or more chemical substances dissolved, i.e., molecularly dispersed, in a suitable solvent or mixture of mutually miscible solvents. For reasons of their ingredients, method of preparation, or use, they do not fall into another group of products.Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Insect Vectors: Insects that transmit infective organisms from one host to another or from an inanimate reservoir to an animate host.Kinetics: The rate dynamics in chemical or physical systems.Alzheimer Disease: A degenerative disease of the BRAIN characterized by the insidious onset of DEMENTIA. Impairment of MEMORY, judgment, attention span, and problem solving skills are followed by severe APRAXIAS and a global loss of cognitive abilities. The condition primarily occurs after age 60, and is marked pathologically by severe cortical atrophy and the triad of SENILE PLAQUES; NEUROFIBRILLARY TANGLES; and NEUROPIL THREADS. (From Adams et al., Principles of Neurology, 6th ed, pp1049-57)Amyloid beta-Peptides: Peptides generated from AMYLOID BETA-PEPTIDES PRECURSOR. An amyloid fibrillar form of these peptides is the major component of amyloid plaques found in individuals with Alzheimer's disease and in aged individuals with trisomy 21 (DOWN SYNDROME). The peptide is found predominantly in the nervous system, but there have been reports of its presence in non-neural tissue.Amyloid beta-Protein Precursor: A single-pass type I membrane protein. It is cleaved by AMYLOID PRECURSOR PROTEIN SECRETASES to produce peptides of varying amino acid lengths. A 39-42 amino acid peptide, AMYLOID BETA-PEPTIDES is a principal component of the extracellular amyloid in SENILE PLAQUES.Plaque, Amyloid: Accumulations of extracellularly deposited AMYLOID FIBRILS within tissues.Disease Models, Animal: Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.Mice, Transgenic: Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.Macrophages: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)Glioma: Benign and malignant central nervous system neoplasms derived from glial cells (i.e., astrocytes, oligodendrocytes, and ependymocytes). Astrocytes may give rise to astrocytomas (ASTROCYTOMA) or glioblastoma multiforme (see GLIOBLASTOMA). Oligodendrocytes give rise to oligodendrogliomas (OLIGODENDROGLIOMA) and ependymocytes may undergo transformation to become EPENDYMOMA; CHOROID PLEXUS NEOPLASMS; or colloid cysts of the third ventricle. (From Escourolle et al., Manual of Basic Neuropathology, 2nd ed, p21)Brain Neoplasms: Neoplasms of the intracranial components of the central nervous system, including the cerebral hemispheres, basal ganglia, hypothalamus, thalamus, brain stem, and cerebellum. Brain neoplasms are subdivided into primary (originating from brain tissue) and secondary (i.e., metastatic) forms. Primary neoplasms are subdivided into benign and malignant forms. In general, brain tumors may also be classified by age of onset, histologic type, or presenting location in the brain.Survivors: Persons who have experienced a prolonged survival after serious disease or who continue to live with a usually life-threatening condition as well as family members, significant others, or individuals surviving traumatic life events.Cyclic AMP Response Element-Binding Protein: A protein that has been shown to function as a calcium-regulated transcription factor as well as a substrate for depolarization-activated CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES. This protein functions to integrate both calcium and cAMP signals.Reproducibility of Results: The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.Prognosis: A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.BooksPublishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.MEDLINE: The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).Serial Publications: Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)Hypothalamic Area, Lateral: Area in the hypothalamus bounded medially by the mammillothalamic tract and the anterior column of the FORNIX (BRAIN). The medial edge of the INTERNAL CAPSULE and the subthalamic region form its lateral boundary. It contains the lateral hypothalamic nucleus, tuberomammillary nucleus, lateral tuberal nuclei, and fibers of the MEDIAL FOREBRAIN BUNDLE.Preoptic Area: Region of hypothalamus between the ANTERIOR COMMISSURE and OPTIC CHIASM.Sleep: A readily reversible suspension of sensorimotor interaction with the environment, usually associated with recumbency and immobility.Arousal: Cortical vigilance or readiness of tone, presumed to be in response to sensory stimulation via the reticular activating system.Histamine: An amine derived by enzymatic decarboxylation of HISTIDINE. It is a powerful stimulant of gastric secretion, a constrictor of bronchial smooth muscle, a vasodilator, and also a centrally acting neurotransmitter.Histidine Decarboxylase: An enzyme that catalyzes the decarboxylation of histidine to histamine and carbon dioxide. It requires pyridoxal phosphate in animal tissues, but not in microorganisms. EC 4.1.1.22.Wakefulness: A state in which there is an enhanced potential for sensitivity and an efficient responsiveness to external stimuli.
Diaminobenzidine). Use of one of these reagents after exposure to the necessary enzyme (e.g., horseradish peroxidase conjugated ... Some examples of substrates used (also known as chromogens) are AEC (3-Amino-9-EthylCarbazole), or DAB (3,3'- ...
Diaminobenzidine or diaminobenzidine, commonly used in immunohistochemical staining 4-Dimethylaminoazobenzene (methyl yellow), ...
... with diaminobenzidine reagent for hemoglobin) and then count individual colonies, each arising from a single plated progenitor ... IL-3 etc.) and more time for growth that will also delay the terminal differentiation and hemoglobinization. Wu H, Liu X, ... and thus can be induced to terminally differentiate in vitro in 2-3 days in the presence of only erythropoietin (Epo) (together ...
... diaminobenzidine is not melted by exposure to a temperature of 770 degrees and loses only 30% of its weight after exposure to ... ISBN 978-3-540-11774-2. Iwakura, Yoshio; Uno, Keikichi; Imai, Yoshio (June 1964). "Polyphenylenebenzimidazoles". Journal of ... and 3,3',4,4'-tetraaminodiphenyl (II) (Figure 1). The spontaneous cyclization of the intermediately formed animo-amide (III) to ... The polymer synthesized from isophthalic acid and 3,3'- ...
... followed by a standard or metal-enhanced diaminobenzidine (DAB) reaction. Samples can then be analyzed by optical microscopy as ... 3 kDa) yields sensitive and detailed retrograde labeling of neuronal cell bodies. Reiner A, Veenman CL, Medina L, Jiao Y, Del ...
In 1969 they developed a staining technique (alkaline diaminobenzidine, or DAB) by which they studied the structure of another ... Novikoff, AB; Goldfischer, S (1969). "Visualization of peroxisomes (microbodies) and mitochondria with diaminobenzidine". The ... 137 (3): 391-7. doi:10.1111/j.1432-1033.1983.tb07841.x. PMID 6319122. Essner, E; Novikoff, AB (1962). "CYTOLOGICAL STUDIES ON ... 3-4. ISBN 9781461465614. Novikoff, AB (1955). "Some aspects of hepatoma NK". Journal of the National Cancer Institute. 15 (5, ...
... diaminobenzidine", issued 1976-03-09, assigned to Celanese Corporation "D.A.B. (Diaminobenzidine)". Chesapeake Bay Division, ... Diaminobenzidine is oxidized by hydrogen peroxide in the presence of hemoglobin to give a dark-brown color. This color change ... Diaminobenzidine, which is commercially available, is prepared by treating 3,3'-dichlorobenzidine with ammonia with a copper ... Diaminobenzidine (DAB) is an organic compound with the formula (C6H3(NH2)2)2. This derivative of benzidine is a precursor to ...
... fluorescent molecules can be utilized to drive the oxidation of diaminobenzidine (DAB) to create a reaction product that can be ... 92-3. ISBN 978-3-642-04609-4. Walton JD; Hallen-Adams He; Luo H (4 August 2010). "Ribosomal biosynthesis of the cyclic peptide ... 162 (3): 583-8. doi:10.1111/j.1432-1033.1987.tb10679.x. PMID 3830158. Wehland J, Osborn M, Weber K (December 1977). "Phalloidin ... 3 (6): 339-350. doi:10.1351/pac196306030339. Schröder, Eberhard; Lübke, Klaus (2014). The Peptides, Volume II: Synthesis, ...
HRP then converts diaminobenzidine (DAB) into an insoluble brown product, which can be detected in a bright-field microscope ... 3-20. doi:10.1007/978-1-60761-789-1_1. ISBN 978-1-60761-788-4. PMID 20809300. Lambros, M. B.; Simpson, P. T.; Jones, C; ... 46 (3): 207-16. PMC 3854374 . PMID 23558859. Kumamoto, H; Sasano, H; Taniguchi, T; Suzuki, T; Moriya, T; Ichinohasama, R (2001 ... 119 (3): 381-7. doi:10.1309/P40P2EAD42PUKDMG. PMID 12645340. Lambros, M. B.; Natrajan, R; Reis-Filho, J. S. (2007). " ...
... diaminobenzidine MeSH D02.455.426.559.389.185.220 --- dianisidine MeSH D02.455.426.559.389.185.226 --- 3,3'-dichlorobenzidine ... 5-amino-3-((5-nitro-2-furyl)vinyl)-1,2,4-oxadiazole MeSH D02.640.600.290 --- fanft MeSH D02.640.600.308 --- furagin MeSH ... 3-oxo-1,5-pentanediyl)bis(n,n-dimethyl-n-2-propenyl-), dibromide MeSH D02.092.146.325 --- p-dimethylaminoazobenzene MeSH ... vitamin k 3 MeSH D02.455.849.291.850 --- taxoids MeSH D02.455.849.291.850.777 --- paclitaxel MeSH D02.455.849.365 --- dolichol ...
... FRS (3 March 1875, Glasgow - 29 September 1955) was a British pathologist. Hubert Turnbull's father was the manager and actuary of the Scottish Widows Fund and Life Assurance Society and his mother was a daughter of the publisher Adam Black. After education at St Ninian's School, Moffat from 1884 to 1888 and at Charterhouse School from 1888 to 1894, Hubert Turnbull matriculated in 1894 at Magdalen College, Oxford, where he graduated B.A. (Oxon.) in 1898. He pursued graduate study at Oxford and in 1899 received Oxford's Hugh Russell Welsh prize for the study of human anatomy and the art of drawing in relation thereto. In 1900 he began further study of medicine at the London Hospital and in 1902 received there M.B., B.Chir. (Oxon.). In 1902 he also received M.A. (Oxon.) and qualified M.R.C.S. and L.R.C.P. From February to June 1903, he was a house physician at London Hospital but, due to illness, from June to November was unable to work. From November 1903 to July 1904, ...
The resignation of outgoing Treasurer Joe Hockey triggered the 2015 North Sydney by-election which was won by Liberal candidate Trent Zimmerman, a controversially pre-selected, former Hockey staffer, with a 48.2 percent primary vote after a larger than predicted 12.8 percent swing against the Turnbull Coalition Government. This was only the second time in North Sydney since federation that the successful Liberal candidate did not obtain a majority of the primary vote and had to rely on preferences after a large double-digit primary vote swing − more than triple that of the 2015 Canning by-election − all in the absence of a Labor candidate, whom have never been successful in the safe Liberal seat.[195]. The Liberal two-candidate vote of 60.2 percent against independent Stephen Ruff compares to the previous election vote of 65.9 percent against Labor.[195] The reduction of 5.7 percent cannot be considered a "two-party/candidate preferred swing" − when a major party is absent, preference ...
Following the passage of same-sex marriage legislation in Australia in 2017, a federal review was commissioned by the Turnbull Government to examine the suitability of current religious freedom protections, specifically within the context of the new Marriage Amendment (Definition and Religious Freedoms) Act 2017.[23] The Religious Freedom Review was chaired by Philip Ruddock, the former Attorney-General for Australia at the time same-sex marriage was banned by the Howard Government in 2004, and had no associated terms of reference.[24] The review panel was composed of Ruddock alongside Jesuit priest Frank Brennan, former judge Annabelle Bennett, Human Rights Commission president Ros Croucher and constitutional lawyer Nicholas Aroney. The expert review panel first met on 10 January 2018, and on 18 May the final review was handed to the Turnbull Government. The Government has declined to release the review in full pending cabinet discussion on its recommendations.[25] The Ruddock review has been ...
The first phone conversation between the United States President Donald Trump and Australian Prime Minister Malcolm Turnbull took place in February 2017 and lasted around 25 minutes.[60] During the call, Trump disagreed with Turnbull on a deal that had been made during President Barack Obama's presidency. The agreement aims to take about 1,250 asylum seekers into the United States, who are currently located on Nauru and Manus Island by Australian authorities.[61] The deal will involve a swap of the 1,250 refugees located on Nauru and Manus with several thousand refugees originating in Honduras, Guatemala, and other Central American nations. Though the details of the trade were not made transparent to the public, a public briefing announced the deal would be applied only to existing refugees and that they would be resettled in America in the coming year.[62][63] On Twitter, February 2, 2017, Trump tweeted that the refugee agreement was a "dumb deal".[64] Notwithstanding the disagreement, Vice ...
Spindle transfer, where the nuclear DNA is transferred to another healthy egg cell leaving the defective mitochondrial DNA behind, is a potential treatment procedure that has been successfully carried out on monkeys.[22][23] Using a similar pronuclear transfer technique, researchers at Newcastle University led by Douglass Turnbull successfully transplanted healthy DNA in human eggs from women with mitochondrial disease into the eggs of women donors who were unaffected.[24][25] In such cases, ethical questions have been raised regarding biological motherhood, since the child receives genes and gene regulatory molecules from two different women. Using genetic engineering in attempts to produce babies free of mitochondrial disease is controversial in some circles and raises important ethical issues.[26][27] A male baby was born in Mexico in 2016 from a mother with Leigh syndrome using spindle transfer.[28] In September 2012 a public consultation was launched in the UK to explore the ethical issues ...
... resumed racing in the spring over 1,400 m (0.87 mi), but while being reasonably competitive, she did not win any major races. Second up at her next start in the Group 3 Stock Stakes, she came from behind to finish fourth, beaten by just over two lengths. She then started 5-1 equal favourite in the Group 2 Turnbull Stakes, where she again raced at the back of the field, before finishing fourth, beaten by only one length. After finishing as a 14-1 outsider in the Caulfield Cup, she began her partnership with Sydney jockey Glen Boss. Coming from near last with 800 m (0.50 mi) to go in the 2400-m race, she finished fourth behind the Lee Freedman-trained Mummify. The first Tuesday in November 2003 was her first Melbourne Cup victory.[2] Starting as an $8 second favourite, Makybe Diva raced at the back of the field until the finishing straight, where jockey Boss picked his way through the field to win by one-and-a-half lengths.[citation needed] In the autumn of 2004, she resumed over 1400 ...
The Kingston Upon Hull Centre for Magnetic Resonance Investigations (CMRI), which opened in 1992, is a magnetic resonance imaging centre located in the city of Kingston upon Hull (Hull) in the East Riding of Yorkshire, England, UK. It is situated in the grounds of the Hull Royal Infirmary hospital in the centre of the city. The centre carries out both cancer research studies, under the auspices of the University of Hull, and clinical scanning, under the auspices of the local NHS trust, Hull and East Yorkshire Hospitals. The centre currently has three MRI scanners; two 1.5 tesla scanners owned by the NHS (a Philips Intera and a GE Signa) and a 3.0 tesla MR 750 GE Signa scanner owned by the University which was installed in January 2009 (one of the first 3.0 tesla whole-body capable systems in Europe). The medical research is carried out under the Directorship of Professor Lindsay W. Turnbull, and is devoted to the application of magnetic resonance imaging and magnetic resonance spectroscopy ...
La HD 10307 és una estrella semblant al sol en massa, temperatura i contingut en metall, situada a uns 42 anys llum de la Terra a la constel·lació d'Andròmeda. Fou identificat en el setembre de 2003 per l'astrobiòloga Margaret Turnbull de la Universitat d'Arizona a Tucson com a un dels candidats propers més prometedors per a hostatjar vida basada en la seva anàlisi de la llista HabCat d'estrelles. ...
An ellipsoid is a surface that may be obtained from a sphere by deforming it by means of directional scalings, or more generally, of an affine transformation. An ellipsoid is a quadric surface; that is, a surface that may be defined as the zero set of a polynomial of degree two in three variables. Among quadric surfaces, an ellipsoid is characterized by either of the two following properties. Every planar cross section is either an ellipse, or is empty, or is reduced to a single point (this explains the name, meaning "ellipse like"). It is bounded, which means that it may be enclosed in a sufficiently large sphere. An ellipsoid has three pairwise perpendicular axes of symmetry which intersect at a center of symmetry, called the center of the ellipsoid. The line segments that are delimited on the axes of symmetry by the ellipsoid are called the principal axes, or simply axes of the ellipsoid. If the three axes have different lengths, the ellipsoid is said to be tri-axial or (rarely) scalene, and ...
Substances, mixtures and exposure circumstances in this list have been classified by the International Agency for Research on Cancer (IARC) as Group 1: The agent (mixture) is carcinogenic to humans. The exposure circumstance entails exposures that are carcinogenic to humans. This category is used when there is sufficient evidence of carcinogenicity in humans. Exceptionally, an agent (mixture) may be placed in this category when evidence of carcinogenicity in humans is less than sufficient but there is sufficient evidence of carcinogenicity in experimental animals and strong evidence in exposed humans that the agent (mixture) acts through a relevant mechanism of carcinogenicity. 2-Naphthylamine Acetaldehyde associated with consumption of alcoholic beverages 4-Aminobiphenyl Aflatoxins Aristolochic acids, and plants containing them Arsenic and inorganic arsenic compounds1 Asbestos Azathioprine Benzene Benzidine, and dyes metabolized to Benzo[a]pyrene Beryllium and beryllium compounds2 Chlornapazine ...
The ability to adhere to medical devices and subsequently form biofilms is a major virulence factor associated with S. haemolyticus.[3][5][13][14] Biofilm formation increases antibiotic resistance[5][13][14] and often leads to persistent infections.[15][16] S. haemolyticus biofilms are not polysaccharide intercellular adhesin (PIA) dependent, and the lack of the ica operon (the gene cluster that encodes the production of PIA) can be used to distinguish S. haemolyticus isolates from other CoNS species.[3][12][14] Biofilm formation is influenced by a variety of factors including carbohydrates, proteins, and extracellular DNA. Detachment assays with NaIO4, proteinase K, or DNase result in 38%, 98%, and 100% detachment, respectively. The high level of detachment associated with DNase treatment has led several authors to suggest a cell-to-surface and/or cell-to-cell adhesion function for extracellular DNA. Biofilm formation also appears to be influenced by the presence of glucose and NaCl. Biofilm ...
Substances, mixtures and exposure circumstances in this list have been classified by the International Agency for Research on Cancer (IARC) as Group 1: The agent (mixture) is carcinogenic to humans. The exposure circumstance entails exposures that are carcinogenic to humans. This category is used when there is sufficient evidence of carcinogenicity in humans. Exceptionally, an agent (mixture) may be placed in this category when evidence of carcinogenicity in humans is less than sufficient but there is sufficient evidence of carcinogenicity in experimental animals and strong evidence in exposed humans that the agent (mixture) acts through a relevant mechanism of carcinogenicity. 2-Naphthylamine Acetaldehyde associated with consumption of alcoholic beverages 4-Aminobiphenyl Aflatoxins Aristolochic acids, and plants containing them Arsenic and inorganic arsenic compounds1 Asbestos Azathioprine Benzene Benzidine, and dyes metabolized to Benzo[a]pyrene Beryllium and beryllium compounds2 Chlornapazine ...
... is often involved in xenobiotic metabolism of substances such as drugs, pollutants, bilirubin, androgens, estrogens, mineralocorticoids, glucocorticoids, fatty acid derivatives, retinoids, and bile acids. These linkages involve glycosidic bonds. Glucuronidation consists of transfer of the glucuronic acid component of uridine diphosphate glucuronic acid to a substrate by any of several types of UDP-glucuronosyltransferase. UDP-glucuronic acid (glucuronic acid linked via a glycosidic bond to uridine diphosphate) is an intermediate in the process and is formed in the liver. One example is the N-glucuronidation of an aromatic amine, 4-aminobiphenyl, by UGT1A4 or UGT1A9 from human, rat, or mouse liver. The substances resulting from glucuronidation are known as glucuronides (or glucuronosides) and are typically much more water-soluble than the non-glucuronic acid-containing substances from which they were originally synthesised. The human body uses glucuronidation to make a large ...
... diaminobenzidine (DAB) assay In vitro localization of H2O2 was determined by DAB assay as described by Kumari et al. (2015). ... Further, 3, 3′ Diaminobenzidin (DAB) analysis detected in-situ H2O2 accumulation. H2O2 was visually detected by staining the ... Table 3. Shoot length, tap root length, number of lateral roots, and total chlorophyll content of three transgenic plants and ... These lines exhibited the predicted Mendelian ratio as 3:1 (Figure ​(Figure4A4A and Table ​Table2).2). Simultaneously, all ...
Diaminobenzidine Tetrahydrochloride hydrate,97%, Contains Up to 10% Water, ACROS Organics™ 5g; Glass bottle 3,3- ... Diaminobenzidine Tetrahydrochloride hydrate,97%, Contains Up to 10% Water, ACROS Organics™ ... Diaminobenzidine Tetrahydrochloride hydrate,97%, Contains Up to 10% Water, ACROS Organics™ ...
... diaminobenzidine", issued 1976-03-09, assigned to Celanese Corporation "D.A.B. (Diaminobenzidine)". Chesapeake Bay Division, ... Diaminobenzidine is oxidized by hydrogen peroxide in the presence of hemoglobin to give a dark-brown color. This color change ... Diaminobenzidine, which is commercially available, is prepared by treating 3,3-dichlorobenzidine with ammonia with a copper ... Diaminobenzidine (DAB) is an organic compound with the formula (C6H3(NH2)2)2. This derivative of benzidine is a precursor to ...
Diaminobenzidine is used to detect fingerprints in blood. 3,3-Diaminobenzidine is used in immunohistology and immunoblotting ... diaminobenzidine chromogen to the light and electron microscopic detection of the luteinizing hormone-releasing hormone system ... diaminobenzidine (DAB)-H2O2-horseradish peroxidase (HRP) has been presented and used for the sensitive detection of ... diaminobenzidine chromogen to the light and electron microscopic detection of the luteinizing hormone-releasing hormone system ...
Diaminobenzidine explanation free. What is 3,3-Diaminobenzidine? Meaning of 3,3-Diaminobenzidine medical term. What does 3,3 ... Looking for online definition of 3,3-Diaminobenzidine in the Medical Dictionary? 3,3- ... Diaminobenzidine). Also found in: Wikipedia. diaminobenzidine. (dī″ă-mēn″ō-bĕn′zĭ-dēn″), DAB. A chromogen, C12H14N4, used in ... diaminobenzidine tetrahydrochloride (DAB) in the presence of urea hydrogen peroxide and Co[Cl.. Low doses of bisphenol A and ...
Chromogen of choice for immunoperoxidase techniques. The brown reaction product is highly insoluble, and will not diffuse from the site of localization.
Diaminobenzidine and peroxidase forms a visible insoluble product. This approach has been used to assay activity of glutamate ... diaminobenzidine-tetrahydrochloride-dihydrate (DAB) was used as a chromogen, for detecting the antigen-antibody complex. It is ... The powder has a shelf-life of 3 years. Store in cool place. Keep container tightly closed in a dry and well-ventilated place. ...
... diaminobenzidine tetrahydrochloride hydrate, for spectrophotometric det. of se. IUPAC Name. 4-(3,4-diaminophenyl)benzene-1,2- ... Diaminobenzidine Tetrahydrochloride hydrate,97%, Contains Up to 10% Water, ACROS Organics™ 1g; Glass bottle ... diaminobenzidine tetrahydrochloride hydrate, 1,1-biphenyl-3,3,4,4-tetraamine tetrahydrochloride hydrate, acmc-209qbm, 3,3- ... diaminobenzidine tetrahydrochloride hydrate, isopac r, 1,1-biphenyl-3,3,4,4-tetraamine tetrahydrochloride xhydrate, 3,3 ...
Selective cytochemical localization of peroxidase, cytochrome oxidase and catalase in rat liver with 3,3-diaminobenzidine ...
Diaminobenzidine is used in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase ...
TMB), (3,3,5,5 Tetramethylbenzidine) 3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative ... 5-Bromo-4-chloro-3-indolyl Phosphate/Nitroblue Tetrazolium)Stabilized Chromogen SolutionSpecially prepared for immunoblotting ...
Magnification: ×25; Chromogen diaminobenzidine).. Discussion. Malignant tumors of the small intestine are very rare in the ... 3.. Solem CA, Harmsen WS, Zinsmeister AR, Loftus EV: Small intestinal adenocarcinoma in Crohns disease. A case control study. ... Figure 3 Strong immunoreactivity for Chromogranin A of carcinoid tumor infiltrating the muscular layer of the intestinal wall ... 3.Istituto di Medicina CardiovascolareUniversity of Milan, Fondazione Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena ...
Mean ± SD (n = 3). (d) The proliferation ability of SW480/IMPDH2 and LoVo/IMPDH2 cells were determined by CCK8 assay. *P , 0.05 ... Mean ± SD (n = 3). *P , 0.05; **P , 0.01. (g) Inhibition of the mTOR activity induced upregulation of E-cadherin and ... Mean ± SD (n = 3). (c and d) IMPDH2 overexpression promoted proliferation ability of SW480 and LoVo cells as determined by ... Mean ± SD (n = 3). Scale bars, 100 μm. (f) IMPDH2 knockdown significantly inhibited the migration ability of HCT116 and SW620 ...
... diaminobenzidine (DAB) tetrahydrochloride in the presence of 0.3 mg/ml urea hydrogen peroxide in 0.05 M Tris buffer and 0.15 M ... Figure 3 Competition of E-HRP binding by estriol, 17β-estradiol, DES, BPA, and o.p′-DDT, all used at 30 μM. The results are ... 3). The pesticide o.p′-DDT, which was weaker in inducing [Ca2+]i signals, presented low competition. However, we should note ... 3,3′-diaminobenzidine;. NE,. norepinephrine;. [Ca2+]i,. intracellular Ca2+ concentration. ...
... diaminobenzidine (DAB) staining was performed on Arabidopsis seedlings sprayed with Pp spores as described (29). For bacterial ... 3B). Collectively, these data suggest that AtrbohD is responsible for most of the ROI produced in response to inoculation of ... 3A). Mutations in atrbohF and atrbohD act redundantly in this assay. atrbohF mutants exhibit reproducibly greater diminution of ... 3A). This corresponds to a time when leaves begin to exhibit macroscopic HR. The atrbohD/F double mutant displayed ...
... diaminobenzidine (DAB) stain. Sections were then lightly counterstained with haematoxylin, dehydrated and slides were mounted ... 3 Histology and immunohistochemistry of CAG-CreERTg/−;Pax6fl/+ tissues after a 12 week chase period. a-d H&E stained sections ... 3.. Hill RE, Favor J, Hogan BLM, Ton CCT, Saunders GF, Hanson IM, Prosser J, Jordan T, Hastie ND, VanHeyningen V. Mouse small ... 3). For comparison, previously published corneal histology and immunostaining are shown for wild-type Pax6+/+ and heterozygous ...
3.. Xu ZS, Chen M, Li LC, Ma YZ. Functions of the ERF transcription factor family in plants. Botany. 2008;86(9):969-77.CrossRef ... 3a). It may suggest that these genes play an important role in soybean roots.. Open image in new window. ... 3b, the ten MYB-related genes presented with an exon-intron structure. The results showed that the MYB-related genes tended to ... 3a). No previous studies have shown that the MYB-related gene is the most expressed in the root and performs some certain ...
3.. Kern SE, Shibata D (2007) The fuzzy math of solid tumor stem cells: a perspective. Cancer Res 67:8985-8988CrossRefPubMed ... 39-diaminobenzidine. NO. Nitric oxide. This is a preview of subscription content, log in to check access. ...
Diaminobenzidine. FFPE:. Formalin-fixed paraffin-embedded. IgH-PCR:. Immunoglobulin heavy chain gene polymerase chain reaction ... Lilla Turiak,1 Bálint Kaszás,2 Krisztián Katona,2,3 Ágnes Lacza,2 László Márk,4 Károly Vékey,1 László Drahos,1 and Tamás ... The ratio of the κ/(κ+λ) light chains in the studied samples is shown in Table 3. Values close to 1 indicate κ dominance, while ... In patients 1, 3, and 5, λ dominance was seen at a ratio of 4x, 19x, and 5x, respectively (Table 1), while in patient 4 6x κ ...
... diaminobenzidine (DAB). Following washing, Mayers hematoxylin was used as counter staining. Finally, the sections were washed ... 3. Results. 3.1. General Histology. The HCM specimens showed variations in cardiomyocyte size and form but the majority of the ... 3, pp. 279-292, 1994. View at: Publisher Site , Google Scholar*J. E. Jalil, W. Doering, J. S. Janicki et al., "Structural vs. ... 3, pp. 668-673, 1990. View at: Google Scholar*A. Waldenström, H. J. Martinussen, B. Gerdin, and R. Hällgren, "Accumulation of ...
... diaminobenzidine (DAB+; K3468, Dako). Sections were counterstained with hematoxylin, dehydrated and mounted in Pertex. For HCA2 ... 3.Department of PathologyLeiden University Medical CenterLeidenThe Netherlands. *4.Department of StatisticsLeiden University ... 3 Kaplan-Meier survival estimates based on CD8+ TILs, stratified on HLA class 1 status. a Effect of CD8+ tumor infiltrate on ... 2 and Online Resource 3).. The expression of HLA class 1 is a prerequisite for CD8+ CTLs to recognize tumor cells [36] and ...
Diaminobenzidine. DMSO. Dimethyl sulphoxide. DNPH. Dinitrophenylhydrazine. MDA. Malondialdehyde,. MTT. 3-(4,5-Dimethylthiazol-2 ... 3.. England K, Cotter T (2005) Direct oxidative modifications of signalling proteins in mammalian cells and their effects on ...
Diaminobenzidine - Similar structures search, synonyms, formulas, resource links, and other chemical information. ... Diaminobenzidine. RN: 91-95-2. UNII: 2RV4T6KHQI. InChIKey: HSTOKWSFWGCZMH-UHFFFAOYSA-N. Note. *. A chemically and ... 1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2. Download. ...
... diaminobenzidine. ... 3⇓). The sustained increase in uptake in the presence of 25 μM ... 3⇑). Involvement of PI3-kinase in the signal transduction pathway of IL-13 is further supported by its activation in epithelial ... FIGURE 3. IL-4 and IL-13 induction of endocytic uptake is inhibited by wortmannin. Shown are representative uptake results from ... 3 Abbreviations used in this paper: MR, mannose receptor; MRM, mannose receptor-mediated; GM-CSF, granulocyte-macrophage CSF; ...
... diaminobenzidine. Catalog Number: SK-4100. Unit Size: 1 kit. *Add to Wishlist ... BCIP/NBT Alkaline Phosphatase (AP) Substrate Kit, 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium. Catalog Number: ... AEC Peroxidase (HRP) Substrate Kit, 3-amino-9-ethylcarbazole Catalog Number: SK-4200 ... TMB Peroxidase (HRP) Substrate Kit, 3,3,5,5-tetramethylbenzidine. Catalog Number: SK-4400 ...
  • Methylcellulose is a semisolid media additive that allows an investigator to stain (with diaminobenzidine reagent for hemoglobin) and then count individual colonies, each arising from a single plated progenitor that is at the CFU-e stage. (wikipedia.org)
  • These manmade chemicals enter the body by ingestion or adsorption and mimic the genomic actions of estrogens ( 3 ) via intracellular estrogen receptors ( 4 - 6 ). (pnas.org)
  • Their chemokine receptor profile lacked the lymph node-homing receptor CCR7, but included the tissue-homing receptors CX 3 CR1 and CXCR3. (nature.com)
  • Among the four subtypes of adenosine receptors, A 1 , A 2A , A 2B , and A 3 ( 15 ), A 1 R and/or A 2A R subtypes have been reported to mediate the sleep-promoting effect of adenosine. (pnas.org)
  • The former is active on all Y receptors and is converted to PYY 3-36 during cleavage by dipeptidase IV. (jneurosci.org)
  • Thus, the functional expression of voltage-dependent L-type Ca 2+ channel (VDCC) is a hallmark of early cardiomyogenesis, whereas IP 3 receptors and sarcoplasmic Ca 2+ -ATPases are expressed before the initiation of cardiomyogenesis. (rupress.org)
  • 13 The blockade of GP IIb/IIIa by abciximab was primarily responsible for the decrease in thrombin generation, but the blockade of platelet α v β 3 receptors by abciximab probably also contributed to the effect. (ahajournals.org)
  • 3 A number of immunohistochemical studies have demonstrated that the immune cell infiltrate of many germ cell tumors includes substantial yet variable numbers of T cells, macrophages, and B cells ( 3 , 4 , 5 ). (jimmunol.org)
  • Stimulation of the insulin and insulin-like growth factor I (IGF-I) receptor activates the phosphoinositide-3-kinase/Akt/mTOR pathway causing pleiotropic cellular effects including an mTOR-dependent loss in insulin receptor substrate-1 expression leading to feedback down-regulation of signaling through the pathway. (aacrjournals.org)
  • In model systems, tumors exhibiting mutational activation of phosphoinositide-3-kinase/Akt kinase, a common event in cancers, are hypersensitive to mTOR inhibitors, including rapamycin. (aacrjournals.org)
  • Activation of phosphoinositide-3-kinase (PI3K)/Akt/mTOR signaling through mutation of pathway components as well as through activation of upstream signaling molecules occurs in a majority of cancers contributing to deregulation of proliferation, resistance to apoptosis, and changes in metabolism characteristic of transformed cells ( 1 , 2 ). (aacrjournals.org)
  • By day 3 post bFGF treatment, inflammatory cells, primarily comprising mononuclear cells, migrated to the perichondrial region, and the proliferation of matrix metalloproteinase 1 positive cells peaked. (springer.com)
  • In mammalian cells, 3 major types of MAPK cascades have been identified: extracellular signal-regulated kinase 1/2 (ERK) 1/2 MAPK, Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) kinase, and p38 kinase. (bloodjournal.org)
  • Mucosal vaccination, in contrast to parenteral vaccination, is of particular interest because it can elicit both systemic and mucosal immune responses, mainly secretory IgA (sIgA) Abs, at the very portal of entry of most infectious pathogens ( 3 ). (jimmunol.org)
  • Actinic keratosis is a well documented disease in humans, which also affects dogs and cats [1-3]. (scirp.org)
  • Lectins are carbohydrate-binding proteins that recognize specific oligosaccharides on glycoproteins or glycolipids ( 3 , 4 ). (jimmunol.org)
  • In the Goto-Kakizaki (GK) rat, a genetic model of type 2 diabetes, the neonatal β-cell mass deficit is considered to be the primary defect leading to basal hyperglycemia, which is detectable for the first time 3 weeks after birth. (diabetesjournals.org)
  • The quantitative real time-PCR results confirmed higher expression of PgeIF4A gene in L1-5, L8-3, and L16-2 plants imposed with drought/salt stress. (pubmedcentralcanada.ca)
  • Exposure of astroglia to PACAP increased the maximal velocity of [ 3 H]glutamate uptake by promoting the expression of GLT-1, GLAST, and GS. (jneurosci.org)
  • Moreover, ghrelin increases the expression of NPY mRNA ( 3 - 5 ), and ICV administration of a NPY Y1 antagonist suppresses the ghrelin-stimulated food intake ( 3 - 5 , 15 ). (diabetesjournals.org)
  • HSP70 and glypican-3 were expressed in HCCs, but minimally in NTs and NLs with no significant difference between expression in NTs and NLs. (mdpi.com)
  • In the hypertrophic heart, the best-characterised ECM components are the collagens I and III [ 3 ]. (hindawi.com)
  • Chinese hamster ovary cells were transiently transfected with an AhR-responsive reporter gene, wild-type mouse AhR, or CA-AhR, treated with 10 nM 2,3,7,8-tetrachlorodibenzo- p -dioxin (TCDD) or vehicle alone (1% DMSO), and analyzed as described ( 9 ). (pnas.org)
  • GATA-1 is found in erythroid cells as well as a few other hematopoietic cell lineages ( 3 - 5 ) and also is expressed in Sertoli cells in the testis ( 6 , 7 ). (pnas.org)
  • Here we show that myristoyl-CM4, a new synthetic analog generated by N-myristoylation of AMPs CM4, had anticancer activity against MCF-7, MDA-MB-231, MX-1 breast cancer cells (IC 50 of 3-6 μM) and MDA-MB-231 xenograft tumors. (frontiersin.org)
  • IP 3 -sensitive intracellular Ca 2+ stores and Ca 2+ -ATPases are present during all stages of differentiation in both GFP-positive and GFP-negative cells. (rupress.org)
  • They include astrocytomas, oligodendrogliomas, and oligoastrocytomas based on type of cells they originate from, with the latter having components of both cell types ( 3 ). (aacrjournals.org)
  • GSK-3β is ubiquitously expressed in eukaryotic cells and is highly enriched in the brain and has been implicated in central nervous system dysfunctions like Alzheimer's disease ( 15 ), schizophrenia ( 16 ), dopamine-associated behaviors ( 17 ), bipolar disorders ( 18 ), and Parkinson's disease ( 19 ). (aacrjournals.org)
  • After isolation islets were loaded by incubation in 5 μM Fluo-3 AM (Molecular Probes) for at least 1 h at room temperature before imaging intracellular calcium. (pnas.org)
  • During the defense response, ROI can inhibit the pathogen by strengthening cell walls via oxidative cross-linking of cell-wall glycoproteins ( 5 ) or by directly killing the pathogen ( 3 ). (pnas.org)
  • Because these isoenzymes were specifically or preferentially expressed in xylem, PXP 3-4 and PXP 5 are suggested to be involved in lignin polymerization. (plantphysiol.org)
  • These tumors are frequently resistant to conventional chemotherapeutic drugs and antibody-based therapies, which poorly penetrate the blood brain barrier ( 2, 3 ). (aacrjournals.org)
  • On the other hand, induction of oxidative metabolic processes by the AhR also can cause the production of highly carcinogenic metabolites, creating a link between AhR activation and chemical carcinogenesis ( 3 ). (pnas.org)
  • Significant analysis of microarrays identified 31 upregulated and 3 downregulated genes specifically in GBMs. (aacrjournals.org)
  • Two of the five isolated peroxidases (PXP 3-4 and PXP 5) could oxidize the lignin monomer analog syringaldazine, an activity previously correlated with lignification in poplar. (plantphysiol.org)
  • Several kinases including Akt can attenuate GSK-3β enzymatic activity by phosphorylating the NH 2 -terminal serine, Ser 9 ( 13 ). (aacrjournals.org)