Very toxic industrial chemicals. They are absorbed through the skin, causing lethal blood, bladder, liver, and kidney damage and are potent, broad-spectrum carcinogens in most species.
Biphenyl compounds substituted in any position by one or more amino groups. Permitted are any substituents except fused rings.
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
Inorganic salts of HYDROGEN CYANIDE containing the -CN radical. The concept also includes isocyanides. It is distinguished from NITRILES, which denotes organic compounds containing the -CN radical.
An enzyme isolated from horseradish which is able to act as an antigen. It is frequently used as a histochemical tracer for light and electron microscopy. Its antigenicity has permitted its use as a combined antigen and marker in experimental immunology.
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
An organization of cells into an organ-like structure. Organoids can be generated in culture. They are also found in certain neoplasms.
An oxidoreductase that catalyzes the conversion of HYDROGEN PEROXIDE to water and oxygen. It is present in many animal cells. A deficiency of this enzyme results in ACATALASIA.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
A plant genus of the family POACEAE. The seed is one of the millets used in EDIBLE GRAIN. It contains vitexin. The common name of buffelgrass is also used for CENCHRUS.
A plant genus of the family POACEAE. The seed is one of the EDIBLE GRAINS used in millet cereals and in feed for birds and livestock (ANIMAL FEED). It contains diosgenin (SAPONINS).
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
A publication issued at stated, more or less regular, intervals.
The largest of the three prokaryotic initiation factors with a molecular size of approximately 80 kD. It functions in the transcription initiation process by promoting the binding of formylmethionine-tRNA to the P-site of the 30S ribosome and by preventing the incorrect binding of elongator tRNA to the translation initiation site.
Institutional committees established to protect the welfare of animals used in research and education. The 1971 NIH Guide for the Care and Use of Laboratory Animals introduced the policy that institutions using warm-blooded animals in projects supported by NIH grants either be accredited by a recognized professional laboratory animal accrediting body or establish its own committee to evaluate animal care; the Public Health Service adopted a policy in 1979 requiring such committees; and the 1985 amendments to the Animal Welfare Act mandate review and approval of federally funded research with animals by a formally designated Institutional Animal Care and Use Committee (IACUC).
Gloves, usually rubber, worn by surgeons, examining physicians, dentists, and other health personnel for the mutual protection of personnel and patient.
The protection of animals in laboratories or other specific environments by promoting their health through better nutrition, housing, and care.
The use of animals as investigational subjects.
A polypeptide that is secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Growth hormone, also known as somatotropin, stimulates mitosis, cell differentiation and cell growth. Species-specific growth hormones have been synthesized.
Hard, amorphous, brittle, inorganic, usually transparent, polymerous silicate of basic oxides, usually potassium or sodium. It is used in the form of hard sheets, vessels, tubing, fibers, ceramics, beads, etc.
A hypnotic and sedative used in the treatment of INSOMNIA.
Polymeric materials (usually organic) of large molecular weight which can be shaped by flow. Plastic usually refers to the final product with fillers, plasticizers, pigments, and stabilizers included (versus the resin, the homogeneous polymeric starting material). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Homogeneous liquid preparations that contain one or more chemical substances dissolved, i.e., molecularly dispersed, in a suitable solvent or mixture of mutually miscible solvents. For reasons of their ingredients, method of preparation, or use, they do not fall into another group of products.
Neoplasms composed of primordial GERM CELLS of embryonic GONADS or of elements of the germ layers of the EMBRYO, MAMMALIAN. The concept does not refer to neoplasms located in the gonads or present in an embryo or FETUS.
Tumors or cancer of the TESTIS. Germ cell tumors (GERMINOMA) of the testis constitute 95% of all testicular neoplasms.
A malignant neoplasm of the germinal tissue of the GONADS; MEDIASTINUM; or pineal region. Germinomas are uniform in appearance, consisting of large, round cells with vesicular nuclei and clear or finely granular eosinophilic-staining cytoplasm. (Stedman, 265th ed; from DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, pp1642-3)
The reproductive cells in multicellular organisms at various stages during GAMETOGENESIS.
A radiosensitive, malignant neoplasm of the testis, thought to be derived from primordial germ cells of the sexually undifferentiated embryonic gonad. There are three variants: classical (typical), the most common type; anaplastic; and spermatocytic. The classical seminoma is composed of fairly well differentiated sheets or cords of uniform polygonal or round cells (seminoma cells), each cell having abundant clear cytoplasm, distinct cell membranes, a centrally placed round nucleus, and one or more nucleoli. In the female, a grossly and histologically identical neoplasm, known as dysgerminoma, occurs. (Dorland, 27th ed)
An unusual and aggressive tumor of germ-cell origin that reproduces the extraembryonic structures of the early embryo. It is the most common malignant germ cell tumor found in children. It is characterized by a labyrinthine glandular pattern of flat epithelial cells and rounded papillary processes with a central capillary (Schiller-Duval body). The tumor is rarely bilateral. Before the use of combination chemotherapy, the tumor was almost invariably fatal. (From DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, p1189)
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Ring compounds having atoms other than carbon in their nuclei. (Grant & Hackh's Chemical Dictionary, 5th ed)
Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed)
Five-membered heterocyclic ring structures containing an oxygen in the 1-position and a nitrogen in the 3-position, in distinction from ISOXAZOLES where they are at the 1,2 positions.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).
A concave exterior region on some POLYCYCLIC AROMATIC HYDROCARBONS that have three phenyl rings in a non-linear arrangement.
Diaminobenzidine). Use of one of these reagents after exposure to the necessary enzyme (e.g., horseradish peroxidase conjugated ... Some examples of substrates used (also known as chromogens) are AEC (3-Amino-9-EthylCarbazole), or DAB (3,3'- ... ISBN 978-1-4419-1304-3. Renshaw, Simon (2017). Immunohistochemistry and Immunocytochemistry: Essential Methods, Second Edition ...
Diaminobenzidine or diaminobenzidine, commonly used in immunohistochemical staining Digital Audio Broadcasting, or DAB, a ...
... with diaminobenzidine reagent for hemoglobin) and then count individual colonies, each arising from a single plated progenitor ... IL-3 etc) and more time for growth that will also delay the terminal differentiation and hemoglobinization. Miller, Cindy L.; ... and thus can be induced to terminally differentiate in vitro in 2-3 days in the presence of only erythropoietin (Epo) (together ...
... diaminobenzidine is not melted by exposure to a temperature of 770°C and loses only 30% of its weight after exposure to high ... 2 (3): 1720-1726. doi:10.1021/acsaem.8b01820. Wainright JS, Wang JT, Weng D, Savinell RF, Litt, M (July 1995). "Acid-doped ... ISBN 978-3-540-11774-2. Iwakura, Yoshio; Uno, Keikichi; Imai, Yoshio (June 1964). "Polyphenylenebenzimidazoles". Journal of ... CS1 maint: discouraged parameter (link) "PBI History". Archived from the original on 3 March 2016. Retrieved 14 February 2014. ...
... followed by a standard or metal-enhanced diaminobenzidine (DAB) reaction. Samples can then be analyzed by optical microscopy as ... 3 kDa) yields sensitive and detailed retrograde labeling of neuronal cell bodies. Reiner A, Veenman CL, Medina L, Jiao Y, Del ...
In 1969 they developed a staining technique (alkaline diaminobenzidine, or DAB) by which they studied the structure of another ... Novikoff, AB; Goldfischer, S (1969). "Visualization of peroxisomes (microbodies) and mitochondria with diaminobenzidine". The ... 137 (3): 391-7. doi:10.1111/j.1432-1033.1983.tb07841.x. PMID 6319122. Essner, E; Novikoff, AB (1962). "CYTOLOGICAL STUDIES ON ... 3-4. ISBN 9781461465614. Novikoff, AB (1955). "Some aspects of hepatoma NK". Journal of the National Cancer Institute. 15 (5, ...
... diaminobenzidine", issued 1976-03-09, assigned to Celanese Corporation "D.A.B. (Diaminobenzidine)". Chesapeake Bay Division, ... Diaminobenzidine is oxidized by hydrogen peroxide in the presence of hemoglobin to give a dark-brown color. This color change ... Diaminobenzidine, which is commercially available, is prepared by treating 3,3′-dichlorobenzidine with ammonia with a copper ... Diaminobenzidine (DAB) is an organic compound with the formula (C6H3(NH2)2)2. This derivative of benzidine is a precursor to ...
... fluorescent molecules can be utilized to drive the oxidation of diaminobenzidine (DAB) to create a reaction product that can be ... 92-3. ISBN 978-3-642-04609-4. Walton JD; Hallen-Adams He; Luo H (4 August 2010). "Ribosomal biosynthesis of the cyclic peptide ... 162 (3): 583-8. doi:10.1111/j.1432-1033.1987.tb10679.x. PMID 3830158. Wehland J, Osborn M, Weber K (December 1977). "Phalloidin ... 3 (6): 339-350. doi:10.1351/pac196306030339. Schröder, Eberhard; Lübke, Klaus (2014). The Peptides, Volume II: Synthesis, ...
HRP then converts diaminobenzidine (DAB) into an insoluble brown product, which can be detected in a bright-field microscope ... 3-20. doi:10.1007/978-1-60761-789-1_1. ISBN 978-1-60761-788-4. PMID 20809300. Lambros, M. B.; Simpson, P. T.; Jones, C; ... 46 (3): 207-16. doi:10.1590/1414-431x20132483. PMC 3854374. PMID 23558859. Kumamoto, H; Sasano, H; Taniguchi, T; Suzuki, T; ... 119 (3): 381-7. doi:10.1309/P40P2EAD42PUKDMG. PMID 12645340. Lambros, M. B.; Natrajan, R; Reis-Filho, J. S. (2007). " ...
APEX can also be used for local deposition of diaminobenzidine, a precursor for an electron microscopy stain. APEX2 is a ... 69 (3): 517-532.e11. doi:10.1016/j.molcel.2017.12.020. ISSN 1097-2765. PMID 29395067. Bersuker, Kirill; Peterson, Clark W. H.; ... 3 (1): 1-13. doi:10.1038/s42003-020-0758-y. ISSN 2399-3642. Tamura, Tomonori; Takato, Mikiko; Shiono, Keiya; Hamachi, Itaru ( ...
Formula: C12H14N4 / (NH2)2C6H3C6H3(NH2)2. Molecular mass: 214.3 Melting point: 176°C Solubility in water: very poor. Flash ... The recommendations on this Card also apply to 3,3-Diaminobenzidine tetrahydrochloride, CAS-number 7411-49-6. ...
... diaminobenzidine", issued 1976-03-09, assigned to Celanese Corporation "D.A.B. (Diaminobenzidine)". Chesapeake Bay Division, ... Diaminobenzidine is oxidized by hydrogen peroxide in the presence of hemoglobin to give a dark-brown color. This color change ... Diaminobenzidine, which is commercially available, is prepared by treating 3,3′-dichlorobenzidine with ammonia with a copper ... Diaminobenzidine (DAB) is an organic compound with the formula (C6H3(NH2)2)2. This derivative of benzidine is a precursor to ...
Diaminobenzidine is used to detect fingerprints in blood. 3,3-Diaminobenzidine is used in immunohistology and immunoblotting ... diaminobenzidine chromogen to the light and electron microscopic detection of the luteinizing hormone-releasing hormone system ... diaminobenzidine (DAB)-H2O2-horseradish peroxidase (HRP) has been presented and used for the sensitive detection of ... diaminobenzidine chromogen to the light and electron microscopic detection of the luteinizing hormone-releasing hormone system ...
Diaminobenzidine explanation free. What is 3,3-Diaminobenzidine? Meaning of 3,3-Diaminobenzidine medical term. What does 3,3 ... Looking for online definition of 3,3-Diaminobenzidine in the Medical Dictionary? 3,3- ... Diaminobenzidine). Also found in: Wikipedia. diaminobenzidine. (dī″ă-mēn″ō-bĕn′zĭ-dēn″), DAB. A chromogen, C12H14N4, used in ... diaminobenzidine tetrahydrochloride (DAB) in the presence of urea hydrogen peroxide and Co[Cl.. Low doses of bisphenol A and ...
Chromogen of choice for immunoperoxidase techniques. The brown reaction product is highly insoluble, and will not diffuse from the site of localization.
Diaminobenzidine and peroxidase forms a visible insoluble product. This approach has been used to assay activity of glutamate ... diaminobenzidine-tetrahydrochloride-dihydrate (DAB) was used as a chromogen, for detecting the antigen-antibody complex. It is ... The powder has a shelf-life of 3 years. Store in cool place. Keep container tightly closed in a dry and well-ventilated place. ...
... diaminobenzidine tetrahydrochloride hydrate, for spectrophotometric det. of se. IUPAC Name. 4-(3,4-diaminophenyl)benzene-1,2- ... Diaminobenzidine Tetrahydrochloride hydrate,97%, Contains Up to 10% Water, ACROS Organics™ 1g; Glass bottle ... diaminobenzidine tetrahydrochloride hydrate, 1,1-biphenyl-3,3,4,4-tetraamine tetrahydrochloride hydrate, acmc-209qbm, 3,3- ... diaminobenzidine tetrahydrochloride hydrate, isopac r, 1,1-biphenyl-3,3,4,4-tetraamine tetrahydrochloride xhydrate, 3,3 ...
Selective cytochemical localization of peroxidase, cytochrome oxidase and catalase in rat liver with 3,3-diaminobenzidine ...
Diaminobenzidine is used in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase ...
... and type-3 (neuroblast) progenitors but not type-1 neural stem cells. These results together suggest that activation of type 2 ... 3 progenitor cells simultaneously. At one week into abstinence, animals in the ethanol exposure groups had an increase in ... Diaminobenzidine Tetrahydrochloride (DAB) Labeled IHC. For antibodies to the neurogenesis-related and cell cycle-related ... Table 2 illustrates that the mean BECs [F(3,27) = 4.17; p , 0.05] and mean WD scores [F(3,29) = 3.09; p , 0.05] were ...
Diaminobenzidine (DAB) Staining of Tobacco Plants. Staining of tobacco leaves from SbDhn1, SbDhn2, VT and NT plants was done ... diaminobenzidine; DCPIP, 2,6-dichlorophenolindophenol; DHE, dihydroethidium; Fv/Fm, maximum quantum yield of PSII; H2O2, ... Enzyme activities were measured at 0 day, 3 days, and 5 days of stress treatment. (A) SOD, (B) POX, (C) APX, and (D) CAT. Error ... All the parameters were collected at 0 day, 3 days, and 5 days of stress treatment. (A) Proline content, (B) MDA content, (C) ...
TMB), (3,3,5,5 Tetramethylbenzidine) 3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative ... 5-Bromo-4-chloro-3-indolyl Phosphate/Nitroblue Tetrazolium)Stabilized Chromogen SolutionSpecially prepared for immunoblotting ...
Magnification: ×25; Chromogen diaminobenzidine).. Discussion. Malignant tumors of the small intestine are very rare in the ... 3.. Solem CA, Harmsen WS, Zinsmeister AR, Loftus EV: Small intestinal adenocarcinoma in Crohns disease. A case control study. ... Figure 3 Strong immunoreactivity for Chromogranin A of carcinoid tumor infiltrating the muscular layer of the intestinal wall ... 3.Istituto di Medicina CardiovascolareUniversity of Milan, Fondazione Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena ...
Mean ± SD (n = 3). (d) The proliferation ability of SW480/IMPDH2 and LoVo/IMPDH2 cells were determined by CCK8 assay. *P , 0.05 ... Mean ± SD (n = 3). *P , 0.05; **P , 0.01. (g) Inhibition of the mTOR activity induced upregulation of E-cadherin and ... Mean ± SD (n = 3). (c and d) IMPDH2 overexpression promoted proliferation ability of SW480 and LoVo cells as determined by ... Mean ± SD (n = 3). Scale bars, 100 μm. (f) IMPDH2 knockdown significantly inhibited the migration ability of HCT116 and SW620 ...
... diaminobenzidine (DAB) tetrahydrochloride in the presence of 0.3 mg/ml urea hydrogen peroxide in 0.05 M Tris buffer and 0.15 M ... Figure 3 Competition of E-HRP binding by estriol, 17β-estradiol, DES, BPA, and o.p′-DDT, all used at 30 μM. The results are ... 3). The pesticide o.p′-DDT, which was weaker in inducing [Ca2+]i signals, presented low competition. However, we should note ... 3,3′-diaminobenzidine;. NE,. norepinephrine;. [Ca2+]i,. intracellular Ca2+ concentration. ...
... diaminobenzidine (DAB) staining was performed on Arabidopsis seedlings sprayed with Pp spores as described (29). For bacterial ... 3B). Collectively, these data suggest that AtrbohD is responsible for most of the ROI produced in response to inoculation of ... 3A). Mutations in atrbohF and atrbohD act redundantly in this assay. atrbohF mutants exhibit reproducibly greater diminution of ... 3A). This corresponds to a time when leaves begin to exhibit macroscopic HR. The atrbohD/F double mutant displayed ...
Epub 2015 Aug 3. Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Govt ... diaminobenzidine ; H&E, hematoxylin and eosin; LPS, lipopolysaccharide; RMs, rhesus macaques; SIV, simian immunodeficiency ... Evans TI1, Li H2, Schafer JL2, Klatt NR3, Hao XP4, Traslavina RP4, Estes JD4, Brenchley JM5, Reeves RK6. ... Figure 3.. CXCL16-producing mDCs correlate with accumulation of microbial products in the liver. A, Correlations of liver LPS ...
... diaminobenzidine (DAB) assay In vitro localization of H2O2 was determined by DAB assay as described by Kumari et al. (2015). ... Further, 3, 3′ Diaminobenzidin (DAB) analysis detected in-situ H2O2 accumulation. H2O2 was visually detected by staining the ... Table 3. Shoot length, tap root length, number of lateral roots, and total chlorophyll content of three transgenic plants and ... These lines exhibited the predicted Mendelian ratio as 3:1 (Figure ​(Figure4A4A and Table ​Table2).2). Simultaneously, all ...
Diaminobenzidine. FFPE:. Formalin-fixed paraffin-embedded. IgH-PCR:. Immunoglobulin heavy chain gene polymerase chain reaction ... Lilla Turiak,1 Bálint Kaszás,2 Krisztián Katona,2,3 Ágnes Lacza,2 László Márk,4 Károly Vékey,1 László Drahos,1 and Tamás ... The ratio of the κ/(κ+λ) light chains in the studied samples is shown in Table 3. Values close to 1 indicate κ dominance, while ... In patients 1, 3, and 5, λ dominance was seen at a ratio of 4x, 19x, and 5x, respectively (Table 1), while in patient 4 6x κ ...
... diaminobenzidine (DAB). Following washing, Mayers hematoxylin was used as counter staining. Finally, the sections were washed ... 3. Results. 3.1. General Histology. The HCM specimens showed variations in cardiomyocyte size and form but the majority of the ... 3, pp. 279-292, 1994. View at: Publisher Site , Google Scholar*J. E. Jalil, W. Doering, J. S. Janicki et al., "Structural vs. ... 3, pp. 668-673, 1990. View at: Google Scholar*A. Waldenström, H. J. Martinussen, B. Gerdin, and R. Hällgren, "Accumulation of ...
... diaminobenzidine (EnVision; DAKO) as chromogen. Nuclei were counterstained using hematoxylin. Pictures were taken with a ... 3. Human brain CD8+ T cells distinctly express surface markers, based on CD69 and CD103 co-expression. a-p Quantification of ... 3.. Mueller, S. N. & Mackay, L. K. Tissue-resident memory T cells: local specialists in immune defence. Nat. Rev. Immunol. 16, ... A low expression of CX3CR1 has been associated with a CD4+ TRM-cell phenotype22. However, the CD4+ T cells we isolated rather ...
Zhongyu Liu,1 Xin Xu,1 Hui-Chen Hsu,1 Albert Tousson,2 Ping-Ar Yang,1 Qi Wu,1 Cunren Liu,1 Shaohua Yu,1 Huang-Ge Zhang,1,3 and ... 3(Suppl.):S133-S140. *Tarner, IH, et al. Retroviral gene therapy of collagen-induced arthritis by local delivery of IL-4. Clin ... Figure 3. CII-DC-AdTRAIL+DOX treatment partly decreased production of anti-CII Ab. The level of anti-CII Ab in the sera of mice ... The endogenous peroxidases were inactivated by incubating the slides with 3% H2O2 at RT for 5 minutes. After washing with H2O, ...
Diaminobenzidine - Similar structures search, synonyms, formulas, resource links, and other chemical information. ... Diaminobenzidine. RN: 91-95-2. UNII: 2RV4T6KHQI. InChIKey: HSTOKWSFWGCZMH-UHFFFAOYSA-N. Note. *. A chemically and ... 1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2. Download. ...
... diaminobenzidine) film was deposited on a platinum disc electrode in dichloromethane containing tetrabuthylammonium perchlorate ... A Novel Polyaniline-Poly(3-Methylthiophene)-Poly(3,3 -Diaminobenzidine) Electrode for the Determination of Dopamine in Human ...
Diaminobenzidine Tetrahydrochloride hydrate,97%, Contains Up to 10% Water, ACROS Organics™ 5g; Glass bottle 3,3- ... Diaminobenzidine Tetrahydrochloride hydrate,97%, Contains Up to 10% Water, ACROS Organics™ ... Diaminobenzidine Tetrahydrochloride hydrate,97%, Contains Up to 10% Water, ACROS Organics™ ...
3,3-Diaminobenzidine (DAB) is definitely a trusted chromogen in histological staining strategies. Posted on February 6, 2019. ... 3,3-Diaminobenzidine (DAB) is definitely a trusted chromogen in histological staining strategies and stained tissues is often ... Launch The chromogen 3,3-Diaminobenzidine (DAB) is often employed being a staining agent in histochemical (HC) and ...
... diaminobenzidine. ... 3⇓). The sustained increase in uptake in the presence of 25 μM ... 3⇑). Involvement of PI3-kinase in the signal transduction pathway of IL-13 is further supported by its activation in epithelial ... FIGURE 3. IL-4 and IL-13 induction of endocytic uptake is inhibited by wortmannin. Shown are representative uptake results from ... 3 Abbreviations used in this paper: MR, mannose receptor; MRM, mannose receptor-mediated; GM-CSF, granulocyte-macrophage CSF; ...
After further washing, immunoperoxidase staining was developed using a diaminobenzidine (DAB) chromogen (DakoCytomation) and ... diaminobenzidine; LCM, laser capture microdissection; FDC, follicular dendritic cell. ... 3⇓). The naive repertoire consisted mostly of the IgM isotype (Fig. 3⇓A) and most had acquired few somatic mutations (Fig. 3⇓B ... 3⇓A) and had acquired extensive somatic mutations (Fig. 3⇓B). Given that the frequency of a specific B cell clone in the ...
... diaminobenzidine (DAB; Vector Laboratories) substrate according to the manufacturers instructions. The slides were counter- ... Figure 3. In vitro functional analysis of MaMIF. (A) Data shows the MaMIF-tautomerase activity under the increasing ... Hamsters are important animal models for studying various infectious diseases of humans2,3,4,5,6,7,8,9. The hamster models of ... 3.. Milazzo, M. L., Eyzaguirre, E. J., Molina, C. P. & Fulhorst, C. F. Maporal viral infection in the Syrian golden hamster: a ...
... diaminobenzidine. Catalog Number: SK-4100. Unit Size: 1 kit. *Add to Wishlist ... BCIP/NBT Alkaline Phosphatase (AP) Substrate Kit, 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium. Catalog Number: ... AEC Peroxidase (HRP) Substrate Kit, 3-amino-9-ethylcarbazole Catalog Number: SK-4200 ... TMB Peroxidase (HRP) Substrate Kit, 3,3,5,5-tetramethylbenzidine. Catalog Number: SK-4400 ...
  • 3,3'-Diaminobenzidine is used in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. (alfa.com)
  • diaminobenzidine (DAB)-H2O2-horseradish peroxidase (HRP) has been presented and used for the sensitive detection of carcinoembryonic antigen (CEA) in human serum. (alfa.com)
  • A new sensitive colorimetric assay for peroxidase using 3,3? (alfa.com)
  • In a positive reaction, the surface of the fish egg was stained brown as a result of the oxidation of 3,3'-diaminobenzidine (substrate) by horseradish peroxidase bound to the egg surface by the antibody. (thefreedictionary.com)
  • Reaction between 3,3'-Diaminobenzidine and peroxidase forms a visible insoluble product. (alfa.com)
  • 3,3'-Diaminobenzidine is used in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity.Slides stained with DAB may be coverslipped in the traditional manner and stored for future reference. (mpbio.com)
  • ER, PR, Ki67, p-ERK and HER2 were detected by immunohistochemistry with biotinylated species-specific secondary antibodies, avidin-linked horseradish peroxidase (HRP) (ABC Kit) and 3,3-diaminobenzidine or SG Blue (Vector Laboratories) HRP chromogen substrate. (bioz.com)
  • 3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. (mpbio.com)
  • 3. Quench endogenous peroxidase for 30 min in 100% methanol + 1% H2O2. (novusbio.com)
  • Bound antibody was detected using avidin-biotin-peroxidase (Vector Labs) and diaminobenzidine (DAB). (cellimagelibrary.org)
  • diaminobenzidine chromogen to the light and electron microscopic detection of the luteinizing hormone-releasing hormone system of the rat brain. (alfa.com)
  • For the most part, the vast majority of microscopic pathology is still done by viewing or imaging one marker at a time in serial sections, using a hematoxylin counterstain and either eosin (H&E) or, for protein IF1C measurements, 3,3'-diaminobenzidine (DAB) as a chromogen. (thefreedictionary.com)
  • Specific primary antibody binding was detected using EnVision+ (Dako North America, Carpinteria, California) with 3,3'-diaminobenzidine tetrahydrochloride as chromogen. (thefreedictionary.com)
  • Slides were again washed and incubated with 3,3'-diaminobenzidine (DAB) chromogen, washed with [H. (thefreedictionary.com)
  • diaminobenzidine-tetrahydrochloride-dihydrate (DAB) was used as a chromogen, for detecting the antigen-antibody complex. (alfa.com)
  • 5-Bromo-4-chloro-3-indolyl Phosphate/Nitroblue Tetrazolium)Stabilized Chromogen SolutionSpecially prepared for immunoblotting procedures only. (mpbio.com)
  • 3,3-Diaminobenzidine (DAB) is definitely a trusted chromogen in histological staining strategies and stained tissues is often found in downstream molecular analyses such as for example quantitative PCR (qPCR). (az628.com)
  • Launch The chromogen 3,3-Diaminobenzidine (DAB) is often employed being a staining agent in histochemical (HC) and immunohistochemical (IHC) techniques performed for scientific and research reasons. (az628.com)
  • 3,3'Diaminobenzidine (DAB) is a widely used chromogen for immunohistochemical staining. (abcam.com)
  • 3,3' Diaminobenzidine (DAB) is a widely used chromogen for immunohistochemical staining. (abcam.com)
  • The catalyzed product was visualized with 3,3′-diaminobenzidine Chromogen Kit and lightly counterstained with Mayer's hematoxylin. (aacrjournals.org)
  • The reaction was revealed by 3,3'-diaminobenzidine and counterstained with hematoxylin. (thefreedictionary.com)
  • Diaminobenzidine is oxidized by hydrogen peroxide in the presence of hemoglobin to give a dark-brown color. (wikipedia.org)
  • E-HRP binding was visualized by the precipitate formed when reacting with 3,3'-diaminobenzidine tetrahydrochloride (DAB) in the presence of urea hydrogen peroxide and Co[Cl. (thefreedictionary.com)
  • Five different poplar xylem peroxidases (PXP 1, PXP 2, PXP 3-4, PXP 5, and PXP 6) were isolated. (plantphysiol.org)
  • Two of the five isolated peroxidases (PXP 3-4 and PXP 5) could oxidize the lignin monomer analog syringaldazine, an activity previously correlated with lignification in poplar. (plantphysiol.org)
  • A combination of the glucose oxidase-diaminobenzidine (DAB) method and the DAB-nickel method can successfully bring out details of immunoreactive structures in immunostained preparations. (nih.gov)
  • After isolation islets were loaded by incubation in 5 μM Fluo-3 AM (Molecular Probes) for at least 1 h at room temperature before imaging intracellular calcium. (pnas.org)
  • Following several additional washes, sections were treated for 10 minutes with freshly prepared reaction mixture, containing a 10 mg tablet of the substrate 3,3'-diaminobenzidine tetrachloride (DAB, Sigma, St. (thefreedictionary.com)
  • Collagen fibres are made up by three protein chains that form a stiff triple-stranded helical structure and are distributed throughout the entire myocardium [ 3 ]. (hindawi.com)
  • 3,3',4,4'-Biphenyltetramin) Formula: C 12 H 14 N 4 Formula Weight: 214.27 CAS #: 91-95-2 Melting Point: 173°C Used as an ultrastructural protein tracer for immuno-cytochemistry. (thomassci.com)
  • Tumor sections were also analyzed for vascular endothelial growth factor and insulin-like growth factor-binding protein 3 protein expression, and a slightly decreased and a moderately increased cytoplasmic immunostaining in silibinin-fed groups were observed as compared with the control group, respectively. (aacrjournals.org)
  • The present study was conducted to investigate the clinical significance of cAMP responsive element binding protein 3 like 1 (CREB3L1) and pleiotrophin (PTN) expression in prognosis of patients with brain gliomas. (portlandpress.com)
  • cAMP responsive element binding protein 3 like 1 (CREB3L1), a member of the unfolded protein response, has recently been identified as a metastasis suppressor in both breast and bladder cancer [ 6 ]. (portlandpress.com)
  • In mammalian cells, 3 major types of MAPK cascades have been identified: extracellular signal-regulated kinase 1/2 (ERK) 1/2 MAPK, Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) kinase, and p38 kinase. (bloodjournal.org)
  • However, AKR1B10 levels are minimal in normal liver tissues (NLs), similar to the 70-kilodalton heat shock protein (HSP70) and glypican-3. (mdpi.com)
  • The 70-kilodalton heat shock protein (HSP70) and glypican-3 (GPC3) are upregulated even in early, well-differentiated HCC [ 3 , 4 ], while accumulations of mutated beta-catenin and p53 are mainly observed in moderately to poorly differentiated HCC [ 5 , 6 ]. (mdpi.com)
  • Vi overvågede synaptic vesikel protein endocytose af fusing en synaptic vesikulære membran proteiner, herunder synaptophysin og VAMP2/synaptobrevin, på den vesikulære lumenal side, med pHluorin, en pH-følsom grøn fluorescerende proteiner, der øger sin Fluorescens intensiteten som pH stiger. (jove.com)
  • Således, en stigning på pHluorin fluorescens intensitet angiver fusion, mens et fald angiver endocytose af mærket synaptic vesikel protein. (jove.com)
  • Upon binding of TRAIL to either DR4 or DR5, a cascade of protein activation occurs, leading to initiator (caspase-8 and caspase-10) and effector (caspase-3) caspase activation ( 16 - 20 ). (aacrjournals.org)
  • We therefore examined human blood vessels exposed to radiotherapy and studied C-reactive protein (CRP) and pentraxin 3 (PTX3), a new marker for adverse cardiovascular outcome dependent on TNF- alpha (TNFα) or interleukin-1beta (IL-1β) expression. (biomedcentral.com)
  • Objective: To evaluate the effects of ischemic postconditioning on expressions of pentraxin-related protein 3 (PTX3) and neutrophil CD11b in the plasma of patients with acute myocardial infarction (AMI) after percutaneous coronary intervention (PCI). (thefreedictionary.com)
  • Transmembrane Emp24 protein transport domain containing 3 (TMED3) is a member of the p24 protein family that serves an important role in the vesicular trafficking of proteins at the secretory endoplasmic reticulum (ER)-Golgi interface, and is involved in different signaling pathways in eukaryotic cells ( 9 , 10 ). (spandidos-publications.com)
  • PMP is classified into low-grade (LG) and high-grade (HG) subtypes ( 2 , 3 ), the five-year survival being 63% for the LG and only 23% for the HG disease ( 4 ). (mcponline.org)
  • 3 A number of immunohistochemical studies have demonstrated that the immune cell infiltrate of many germ cell tumors includes substantial yet variable numbers of T cells, macrophages, and B cells ( 3 , 4 , 5 ). (jimmunol.org)
  • 16 , 17 Crabb and colleagues 3 , 18 , 19 have shown that oxidatively modified (carboxyethylpyrrole [CEP]-adducted) proteins are more abundant in the eyes and plasma of AMD patients than of controls. (arvojournals.org)
  • By day 3 post bFGF treatment, inflammatory cells, primarily comprising mononuclear cells, migrated to the perichondrial region, and the proliferation of matrix metalloproteinase 1 positive cells peaked. (springer.com)
  • 7 8 We have previously shown a persistent increase in lamina propria mononuclear cells, immunoglobulin A plasma cells, and cytokine mRNAs in apparently normal mucosa, 2-3 months following acute bacterial colitis. (bmj.com)
  • These manmade chemicals enter the body by ingestion or adsorption and mimic the genomic actions of estrogens ( 3 ) via intracellular estrogen receptors ( 4 - 6 ). (pnas.org)
  • Their chemokine receptor profile lacked the lymph node-homing receptor CCR7, but included the tissue-homing receptors CX 3 CR1 and CXCR3. (nature.com)
  • Thus, the functional expression of voltage-dependent L-type Ca 2+ channel (VDCC) is a hallmark of early cardiomyogenesis, whereas IP 3 receptors and sarcoplasmic Ca 2+ -ATPases are expressed before the initiation of cardiomyogenesis. (rupress.org)
  • 13 The blockade of GP IIb/IIIa by abciximab was primarily responsible for the decrease in thrombin generation, but the blockade of platelet α v β 3 receptors by abciximab probably also contributed to the effect. (ahajournals.org)
  • GK rats were treated with daily injections of glucagon-like peptide-1 (400 μg · kg −1 · day −1 ) or Ex-4 (3 μg · kg −1 · day −1 ) from day 2 to day 6 after birth and were evaluated against Wistar and untreated GK rats. (diabetesjournals.org)
  • IL-4 and IL-13 enhanced fluid phase pinocytosis and mannose receptor-mediated uptake by activation of phosphatidylinositol 3-kinase. (jimmunol.org)
  • Mannose receptor (MR) 3 -mediated (MRM) endocytosis provides an important route for uptake of a broad range of mannosylated glycoproteins and phagocytosed particles ( 6 ). (jimmunol.org)
  • NAGly is believed to activate the cannabinoid receptor GPR18 with no affinity for cannabinoid receptor (CB) 1 [ 1 , 2 , 3 ] and transient receptor potential vanilloid 1 (TRPV1) [ 1 ]. (mdpi.com)
  • 3 In the ERK1/2 (p44/42) MAPK pathway, activation of receptor tyrosine kinases by growth factors induces son of sevenless (SOS) translocation to the plasma membrane and phosphorylation and activation of a cascade of kinases, including Ras, Raf, MAP/ERK kinase (MEK), and ERK. (bloodjournal.org)
  • 8 The addition of the mouse-human chimeric monoclonal antibody abciximab (c7E3 Fab, ReoPro), which blocks GP IIb/IIIa and the related α v β 3 receptor, 8 9 to a regimen of aspirin and heparin decreases early postprocedural complications of PCI and improves the short-term clinical outcome. (ahajournals.org)
  • The antibody enhances the binding of 125 I insulin binding to the insulin receptor of HIR3.5/3T3 cells and stimulates insulin mediated 3 H thymidine incorporation in these cells. (abcam.com)
  • A gene on chromosome 16p11.2 that encodes the integrin alpha M chain, which combines with the beta-2 chain (ITGB2) to form a leukocyte-specific integrin, macrophage receptor 1 (Mac-1), or inactivated C3b receptor 3 (CR3). (thefreedictionary.com)
  • A polyaniline-poly(3-methylthiophene)-poly(3,3 '-diaminobenzidine) film was deposited on a platinum disc electrode in dichloromethane containing tetrabuthylammonium perchlorate supporting electrolyte and used for the amperometric determination of dopamine. (erdogan.edu.tr)
  • Brain dopamine (DA) has been implicated in central responses to intake of palatable sugars through proposed hedonic 1,2 , effort-related 3 and habit-based 4,5 mechanisms of action. (jove.com)
  • MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine), which damages dopamine nerve endings and cell bodies, causes extensive microglial activation in striatum as well as in the substantia nigra. (aspetjournals.org)
  • Actinic keratosis is a well documented disease in humans, which also affects dogs and cats [1-3]. (scirp.org)
  • Additionally, there were more cytotoxic CD8^sup +^ T cells in the tumors of the GTPs+UVB group than in the UVB group and their tumor cells exhibited greater activation of caspase-3, indicating the apoptotic death of the tumor cells. (herbs.org)
  • Combination treatment in vitro produced enhanced caspase-3 and caspase-8 activation. (aacrjournals.org)
  • To further characterize the individual subtypes of neural progenitor cells (NPCs) affected by adolescent binge ethanol exposure, a fluorescent quadruple labeling technique was utilized to differentiate type 1, 2a, 2b, and 3 progenitor cells simultaneously. (frontiersin.org)
  • At one week into abstinence, animals in the ethanol exposure groups had an increase in proliferating type 2 (intermediate progenitors) and type 3 (neuroblast) progenitors but not type 1 neural stem cells. (frontiersin.org)
  • Exposure of astroglia to PACAP increased the maximal velocity of [ 3 H]glutamate uptake by promoting the expression of GLT-1, GLAST, and GS. (jneurosci.org)
  • 3,3′-Diaminobenzidine (DAB) is an organic compound with the formula (C6H3(NH2)2)2. (wikipedia.org)
  • These tumors are frequently resistant to conventional chemotherapeutic drugs and antibody-based therapies, which poorly penetrate the blood brain barrier ( 2, 3 ). (aacrjournals.org)
  • The cells were then incubated with 20 µM heme for a period of 3 (white) or 20 (grey) hours. (bioz.com)
  • IP 3 -sensitive intracellular Ca 2+ stores and Ca 2+ -ATPases are present during all stages of differentiation in both GFP-positive and GFP-negative cells. (rupress.org)
  • They include astrocytomas, oligodendrogliomas, and oligoastrocytomas based on type of cells they originate from, with the latter having components of both cell types ( 3 ). (aacrjournals.org)
  • Staining of mtHSP 70 in DAP.3 cells results in a worm-like staining pattern, consistent with mitochondrial localization. (abcam.com)
  • Significant analysis of microarrays identified 31 upregulated and 3 downregulated genes specifically in GBMs. (aacrjournals.org)
  • Sections are representative of 3 naive and 5 chronically SIV-infected animals. (nih.gov)
  • Although stage-I and -II OSCCs are treated with surgery or radiotherapy, advanced stage-III and -IV disease is treated with a combination of surgery, radiotherapy and chemotherapy ( 3 ). (spandidos-publications.com)
  • The quantitative real time-PCR results confirmed higher expression of PgeIF4A gene in L1-5, L8-3, and L16-2 plants imposed with drought/salt stress. (pubmedcentralcanada.ca)
  • Moreover, ghrelin increases the expression of NPY mRNA ( 3 - 5 ), and ICV administration of a NPY Y1 antagonist suppresses the ghrelin-stimulated food intake ( 3 - 5 , 15 ). (diabetesjournals.org)
  • This change allows calculation of the rCBV, a measure that has been shown to correlate with glioma vascularity 2 , 3 and the expression of VEGF. (ajnr.org)
  • The aim of this study was to evaluate the expression of AKR1B10, HSP70, and glypican-3 in 61 HCC tissue samples compared to corresponding non-tumorous liver tissues (NTs), comprising 42 chronic hepatitis and 19 cirrhosis cases to clarify the significance of molecular changes at the preneoplastic stages of HCC. (mdpi.com)
  • HSP70 and glypican-3 were expressed in HCCs, but minimally in NTs and NLs with no significant difference between expression in NTs and NLs. (mdpi.com)
  • At present, surgeries (e.g. hysterectomy and myomectomy by hysteroscopy or laparoscopy) and pharmacotherapies (e.g. oral contraceptives, progestins, and GnRH analogs) represent the first-line therapeutic approaches for patients diagnosed with uterine leiomyoma [ 3 , 4 ]. (portlandpress.com)
  • 3-5 In bone regeneration, many material fabrication approaches have been implemented for the production of functional substrates able to interact and properly integrate with the host tissue and/or trigger cell differentiation towards osteoblastic lineage. (rsc.org)
  • N-arachidonoyl glycine (NAGly) is a product of the oxidative metabolism of anandamide and shares a structural similarity with this endocannabinoid [ 1 , 2 , 3 ]. (mdpi.com)
  • We found αA-crystallin gene mutations in all 19 F0-generation pups (100%) with indel mutations in the αA-crystallin gene ranging from 3 to 52 bp. (arvojournals.org)
  • 3, 5- 8 The use of resin embedded semithin sections results in improved cytomorphology compared with that seen in paraffin wax embedded sections. (bmj.com)
  • Some examples of substrates used (also known as chromogens) are AEC (3-Amino-9-EthylCarbazole), or DAB (3,3'-Diaminobenzidine). (wikipedia.org)
  • 3 Departamento de Nutrição e Pastagem, Instituto de Zootecnia, Universidade Federal Rural do Rio de Janeiro (UFRRJ), BR-465 Km 7, Seropédica, RJ 23890-000, Brazil. (scielo.br)