'3,3'-Diaminobenzidine (DAB) is a chemical compound used in histology and immunohistochemistry as a chromogen for the visualization of an antigen-antibody reaction, where it forms an insoluble brown precipitate at the site of the reaction, facilitating microscopic analysis.
Very toxic industrial chemicals. They are absorbed through the skin, causing lethal blood, bladder, liver, and kidney damage and are potent, broad-spectrum carcinogens in most species.
Biphenyl compounds substituted in any position by one or more amino groups. Permitted are any substituents except fused rings.
Peroxidases are enzymes that catalyze the reduction of hydrogen peroxide to water, while oxidizing various organic and inorganic compounds, playing crucial roles in diverse biological processes including stress response, immune defense, and biosynthetic reactions.
Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.
Inorganic salts of HYDROGEN CYANIDE containing the -CN radical. The concept also includes isocyanides. It is distinguished from NITRILES, which denotes organic compounds containing the -CN radical.
An enzyme isolated from horseradish which is able to act as an antigen. It is frequently used as a histochemical tracer for light and electron microscopy. Its antigenicity has permitted its use as a combined antigen and marker in experimental immunology.
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
An organization of cells into an organ-like structure. Organoids can be generated in culture. They are also found in certain neoplasms.
An oxidoreductase that catalyzes the conversion of HYDROGEN PEROXIDE to water and oxygen. It is present in many animal cells. A deficiency of this enzyme results in ACATALASIA.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.

3,3'-Diaminobenzidine (DAB) is a chemical compound that is commonly used as a chromogen in histological and immunohistochemical staining techniques. It is a type of polymerization substrate that reacts with horseradish peroxidase (HRP) to produce an insoluble, dark-brown precipitate at the site of the antigen-antibody reaction. This allows for the visualization and localization of specific proteins or other antigens within tissue sections.

The chemical formula for DAB is C12H12N2O2, and it is a light-sensitive compound that should be handled and stored in a dark environment to prevent unwanted photochemical reactions. It is important to note that DAB is considered a potential carcinogen and should be handled with appropriate safety precautions, including the use of gloves, lab coats, and eye protection.

Benzidines are a class of chemical compounds with the basic structure of two benzene rings linked by a central nitrogen atom. The term "benzidine" can refer specifically to the parent compound, but it is more commonly used as a general term for a group of related compounds known as benzidine congeners or benzidine derivatives.

Benzidines are primarily used in the manufacture of dyes and pigments, although they have also been used in some industrial and laboratory applications. Exposure to benzidines has been linked to an increased risk of bladder cancer and other health problems, so their use is regulated in many countries.

It's worth noting that the medical definition of "benzidines" primarily focuses on their chemical structure and potential health effects, rather than their specific medical uses or applications.

Aminobiphenyl compounds are a group of chemical substances that contain two phenyl rings linked by a single carbon-nitrogen bond. The amino group (-NH2) is attached to one of the phenyl rings.

These compounds have been historically used in the manufacture of dyes and were also used as rubber accelerators. However, they have been largely phased out due to their carcinogenic properties. Exposure to certain aminobiphenyl compounds has been associated with an increased risk of bladder cancer in humans.

It is important to note that the medical definition of 'aminobiphenyl compounds' generally refers to their chemical structure and potential health hazards, rather than a specific medical condition or treatment.

Peroxidases are a group of enzymes that catalyze the oxidation of various substrates using hydrogen peroxide (H2O2) as the electron acceptor. These enzymes contain a heme prosthetic group, which plays a crucial role in their catalytic activity. Peroxidases are widely distributed in nature and can be found in plants, animals, and microorganisms. They play important roles in various biological processes, including defense against oxidative stress, lignin degradation, and host-pathogen interactions. Some common examples of peroxidases include glutathione peroxidase, which helps protect cells from oxidative damage, and horseradish peroxidase, which is often used in laboratory research.

Histochemistry is the branch of pathology that deals with the microscopic localization of cellular or tissue components using specific chemical reactions. It involves the application of chemical techniques to identify and locate specific biomolecules within tissues, cells, and subcellular structures. This is achieved through the use of various staining methods that react with specific antigens or enzymes in the sample, allowing for their visualization under a microscope. Histochemistry is widely used in diagnostic pathology to identify different types of tissues, cells, and structures, as well as in research to study cellular and molecular processes in health and disease.

Cyanides are a group of chemical compounds that contain the cyano group, -CN, which consists of a carbon atom triple-bonded to a nitrogen atom. They are highly toxic and can cause rapid death due to the inhibition of cellular respiration. Cyanide ions (CN-) bind to the ferric iron in cytochrome c oxidase, a crucial enzyme in the electron transport chain, preventing the flow of electrons and the production of ATP, leading to cellular asphyxiation.

Common sources of cyanides include industrial chemicals such as hydrogen cyanide (HCN) and potassium cyanide (KCN), as well as natural sources like certain fruits, nuts, and plants. Exposure to high levels of cyanides can occur through inhalation, ingestion, or skin absorption, leading to symptoms such as headache, dizziness, nausea, vomiting, rapid heartbeat, seizures, coma, and ultimately death. Treatment for cyanide poisoning typically involves the use of antidotes that bind to cyanide ions and convert them into less toxic forms, such as thiosulfate and rhodanese.

Horseradish peroxidase (HRP) is not a medical term, but a type of enzyme that is derived from the horseradish plant. In biological terms, HRP is defined as a heme-containing enzyme isolated from the roots of the horseradish plant (Armoracia rusticana). It is widely used in molecular biology and diagnostic applications due to its ability to catalyze various oxidative reactions, particularly in immunological techniques such as Western blotting and ELISA.

HRP catalyzes the conversion of hydrogen peroxide into water and oxygen, while simultaneously converting a variety of substrates into colored or fluorescent products that can be easily detected. This enzymatic activity makes HRP a valuable tool in detecting and quantifying specific biomolecules, such as proteins and nucleic acids, in biological samples.

'Staining and labeling' are techniques commonly used in pathology, histology, cytology, and molecular biology to highlight or identify specific components or structures within tissues, cells, or molecules. These methods enable researchers and medical professionals to visualize and analyze the distribution, localization, and interaction of biological entities, contributing to a better understanding of diseases, cellular processes, and potential therapeutic targets.

Medical definitions for 'staining' and 'labeling' are as follows:

1. Staining: A process that involves applying dyes or stains to tissues, cells, or molecules to enhance their contrast and reveal specific structures or components. Stains can be categorized into basic stains (which highlight acidic structures) and acidic stains (which highlight basic structures). Common staining techniques include Hematoxylin and Eosin (H&E), which differentiates cell nuclei from the surrounding cytoplasm and extracellular matrix; special stains, such as PAS (Periodic Acid-Schiff) for carbohydrates or Masson's trichrome for collagen fibers; and immunostains, which use antibodies to target specific proteins.
2. Labeling: A process that involves attaching a detectable marker or tag to a molecule of interest, allowing its identification, quantification, or tracking within a biological system. Labels can be direct, where the marker is directly conjugated to the targeting molecule, or indirect, where an intermediate linker molecule is used to attach the label to the target. Common labeling techniques include fluorescent labels (such as FITC, TRITC, or Alexa Fluor), enzymatic labels (such as horseradish peroxidase or alkaline phosphatase), and radioactive labels (such as ³²P or ¹⁴C). Labeling is often used in conjunction with staining techniques to enhance the specificity and sensitivity of detection.

Together, staining and labeling provide valuable tools for medical research, diagnostics, and therapeutic development, offering insights into cellular and molecular processes that underlie health and disease.

Electron microscopy (EM) is a type of microscopy that uses a beam of electrons to create an image of the sample being examined, resulting in much higher magnification and resolution than light microscopy. There are several types of electron microscopy, including transmission electron microscopy (TEM), scanning electron microscopy (SEM), and reflection electron microscopy (REM).

In TEM, a beam of electrons is transmitted through a thin slice of the sample, and the electrons that pass through the sample are focused to form an image. This technique can provide detailed information about the internal structure of cells, viruses, and other biological specimens, as well as the composition and structure of materials at the atomic level.

In SEM, a beam of electrons is scanned across the surface of the sample, and the electrons that are scattered back from the surface are detected to create an image. This technique can provide information about the topography and composition of surfaces, as well as the structure of materials at the microscopic level.

REM is a variation of SEM in which the beam of electrons is reflected off the surface of the sample, rather than scattered back from it. This technique can provide information about the surface chemistry and composition of materials.

Electron microscopy has a wide range of applications in biology, medicine, and materials science, including the study of cellular structure and function, disease diagnosis, and the development of new materials and technologies.

Organoids are 3D tissue cultures grown from stem cells that mimic the structure and function of specific organs. They are used in research to study development, disease, and potential treatments. The term "organoid" refers to the fact that these cultures can organize themselves into structures that resemble rudimentary organs, with differentiated cell types arranged in a pattern similar to their counterparts in the body. Organoids can be derived from various sources, including embryonic stem cells, induced pluripotent stem cells (iPSCs), or adult stem cells, and they provide a valuable tool for studying complex biological processes in a controlled laboratory setting.

Catalase is a type of enzyme that is found in many living organisms, including humans. Its primary function is to catalyze the decomposition of hydrogen peroxide (H2O2) into water (H2O) and oxygen (O2). This reaction helps protect cells from the harmful effects of hydrogen peroxide, which can be toxic at high concentrations.

The chemical reaction catalyzed by catalase can be represented as follows:

H2O2 + Catalase → H2O + O2 + Catalase

Catalase is a powerful antioxidant enzyme that plays an important role in protecting cells from oxidative damage. It is found in high concentrations in tissues that produce or are exposed to hydrogen peroxide, such as the liver, kidneys, and erythrocytes (red blood cells).

Deficiency in catalase activity has been linked to several diseases, including cancer, neurodegenerative disorders, and aging. On the other hand, overexpression of catalase has been shown to have potential therapeutic benefits in various disease models, such as reducing inflammation and oxidative stress.

Immunoenzyme techniques are a group of laboratory methods used in immunology and clinical chemistry that combine the specificity of antibody-antigen reactions with the sensitivity and amplification capabilities of enzyme reactions. These techniques are primarily used for the detection, quantitation, or identification of various analytes (such as proteins, hormones, drugs, viruses, or bacteria) in biological samples.

In immunoenzyme techniques, an enzyme is linked to an antibody or antigen, creating a conjugate. This conjugate then interacts with the target analyte in the sample, forming an immune complex. The presence and amount of this immune complex can be visualized or measured by detecting the enzymatic activity associated with it.

There are several types of immunoenzyme techniques, including:

1. Enzyme-linked Immunosorbent Assay (ELISA): A widely used method for detecting and quantifying various analytes in a sample. In ELISA, an enzyme is attached to either the capture antibody or the detection antibody. After the immune complex formation, a substrate is added that reacts with the enzyme, producing a colored product that can be measured spectrophotometrically.
2. Immunoblotting (Western blot): A method used for detecting specific proteins in a complex mixture, such as a protein extract from cells or tissues. In this technique, proteins are separated by gel electrophoresis and transferred to a membrane, where they are probed with an enzyme-conjugated antibody directed against the target protein.
3. Immunohistochemistry (IHC): A method used for detecting specific antigens in tissue sections or cells. In IHC, an enzyme-conjugated primary or secondary antibody is applied to the sample, and the presence of the antigen is visualized using a chromogenic substrate that produces a colored product at the site of the antigen-antibody interaction.
4. Immunofluorescence (IF): A method used for detecting specific antigens in cells or tissues by employing fluorophore-conjugated antibodies. The presence of the antigen is visualized using a fluorescence microscope.
5. Enzyme-linked immunosorbent assay (ELISA): A method used for detecting and quantifying specific antigens or antibodies in liquid samples, such as serum or culture supernatants. In ELISA, an enzyme-conjugated detection antibody is added after the immune complex formation, and a substrate is added that reacts with the enzyme to produce a colored product that can be measured spectrophotometrically.

These techniques are widely used in research and diagnostic laboratories for various applications, including protein characterization, disease diagnosis, and monitoring treatment responses.

Immunohistochemistry (IHC) is a technique used in pathology and laboratory medicine to identify specific proteins or antigens in tissue sections. It combines the principles of immunology and histology to detect the presence and location of these target molecules within cells and tissues. This technique utilizes antibodies that are specific to the protein or antigen of interest, which are then tagged with a detection system such as a chromogen or fluorophore. The stained tissue sections can be examined under a microscope, allowing for the visualization and analysis of the distribution and expression patterns of the target molecule in the context of the tissue architecture. Immunohistochemistry is widely used in diagnostic pathology to help identify various diseases, including cancer, infectious diseases, and immune-mediated disorders.

... diaminobenzidine", issued 1976-03-09, assigned to Celanese Corporation "D.A.B. (Diaminobenzidine)". Chesapeake Bay Division, ... Diaminobenzidine is oxidized by hydrogen peroxide in the presence of hemoglobin to give a dark-brown color. This color change ... Diaminobenzidine, which is commercially available, is prepared by treating 3,3′-dichlorobenzidine with ammonia with a copper ... Diaminobenzidine (DAB) is an organic compound with the formula (C6H3(NH2)2)2. This derivative of benzidine is a precursor to ...
"Axotomy of single fluorescent nerve fibers in developing mammalian spinal cord by photoconversion of diaminobenzidine". J ... 225 (3): 637-56. doi:10.1113/jphysiol.1972.sp009961. PMC 1331135. PMID 4342522. S2CID 32192574. Fuchs, P. A.; Henderson, L. P ...
Diaminobenzidine). Use of one of these reagents after exposure to the necessary enzyme (e.g., horseradish peroxidase conjugated ... Some examples of substrates used (also known as chromogens) are AEC (3-Amino-9-EthylCarbazole), or DAB (3,3'- ... ISBN 978-1-4419-1304-3. Renshaw, Simon (2017). Immunohistochemistry and Immunocytochemistry: Essential Methods, Second Edition ...
Keefer, Donald A.; Christ, Jacob F. (November 1976). "Distribution of endogenous diaminobenzidine-staining cells in the normal ... 133 (3): 510-22. doi:10.1016/j.cell.2008.02.046. PMC 2394859. PMID 18455991. Cakir T, Alsan S, Saybaşili H, Akin A, Ulgen KO ( ... 12 (3): 342-53. doi:10.1016/j.stem.2012.12.015. PMC 3700554. PMID 23472873. Brancaccio M, Edwards MD, Patton AP, Smyllie NJ, ... 62 (3): 557-62. doi:10.1016/S0031-9384(97)00018-8. PMID 9272664. S2CID 41925078. Frot M, Magnin M, Mauguière F, Garcia-Larrea L ...
Diaminobenzidine or diaminobenzidine, commonly used in immunohistochemical staining 1,4-Diaminobutane 10-Deacetylbaccatin, or ...
... with diaminobenzidine reagent for hemoglobin) and then count individual colonies, each arising from a single plated progenitor ... IL-3 etc) and more time for growth that will also delay the terminal differentiation and hemoglobinization. Miller, Cindy L.; ... and thus can be induced to terminally differentiate in vitro in 2-3 days in the presence of only erythropoietin (Epo) (together ...
... diaminobenzidine is not melted by exposure to a temperature of 770 °C and loses only 30% of its weight after exposure to high ... 2 (3): 1720-1726. doi:10.1021/acsaem.8b01820. S2CID 140074080. Wainright JS, Wang JT, Weng D, Savinell RF, Litt, M (July 1995 ... ISBN 978-3-540-11774-2. Iwakura, Yoshio; Uno, Keikichi; Imai, Yoshio (June 1964). "Polyphenylenebenzimidazoles". Journal of ... "PBI History". Archived from the original on 3 March 2016. Retrieved 14 February 2014. Haertsch, Emilie; Meyer, Michal (2016). " ...
... followed by a standard or metal-enhanced diaminobenzidine (DAB) reaction. Samples can then be analyzed by optical microscopy as ... 3 kDa) yields sensitive and detailed retrograde labeling of neuronal cell bodies. Reiner A, Veenman CL, Medina L, Jiao Y, Del ...
In 1969 they developed a staining technique (alkaline diaminobenzidine, or DAB) by which they studied the structure of another ... Novikoff, AB; Goldfischer, S (1969). "Visualization of peroxisomes (microbodies) and mitochondria with diaminobenzidine". The ... 137 (3): 391-7. doi:10.1111/j.1432-1033.1983.tb07841.x. PMID 6319122. Essner, E; Novikoff, AB (1962). "CYTOLOGICAL STUDIES ON ... 3-4. ISBN 9781461465614. Novikoff, AB (1955). "Some aspects of hepatoma NK". Journal of the National Cancer Institute. 15 (5, ...
... fluorescent molecules can be utilized to drive the oxidation of diaminobenzidine (DAB) to create a reaction product that can be ... 92-3. ISBN 978-3-642-04609-4. Walton JD; Hallen-Adams He; Luo H (4 August 2010). "Ribosomal biosynthesis of the cyclic peptide ... 162 (3): 583-8. doi:10.1111/j.1432-1033.1987.tb10679.x. PMID 3830158. Wehland J, Osborn M, Weber K (December 1977). "Phalloidin ... 3 (6): 339-350. doi:10.1351/pac196306030339. Schröder, Eberhard; Lübke, Klaus (2014). The Peptides, Volume II: Synthesis, ...
HRP then converts diaminobenzidine (DAB) into an insoluble brown product, which can be detected in a bright-field microscope ... 3-20. doi:10.1007/978-1-60761-789-1_1. ISBN 978-1-60761-788-4. PMID 20809300. Lambros, M. B.; Simpson, P. T.; Jones, C; ... 46 (3): 207-16. doi:10.1590/1414-431x20132483. PMC 3854374. PMID 23558859. Kumamoto, H; Sasano, H; Taniguchi, T; Suzuki, T; ... 119 (3): 381-7. doi:10.1309/P40P2EAD42PUKDMG. PMID 12645340. Lambros, M. B.; Natrajan, R; Reis-Filho, J. S. (2007). " ...
... diaminobenzidine MeSH D02.455.426.559.389.185.220 - dianisidine MeSH D02.455.426.559.389.185.226 - 3,3'-dichlorobenzidine MeSH ... 5-amino-3-((5-nitro-2-furyl)vinyl)-1,2,4-oxadiazole MeSH D02.640.600.290 - fanft MeSH D02.640.600.308 - furagin MeSH D02.640. ... 3-oxo-1,5-pentanediyl)bis(n,n-dimethyl-n-2-propenyl-), dibromide MeSH D02.092.146.325 - p-dimethylaminoazobenzene MeSH D02.092. ... vitamin k 3 MeSH D02.455.849.291.850 - taxoids MeSH D02.455.849.291.850.777 - paclitaxel MeSH D02.455.849.365 - dolichol MeSH ...
APEX can also be used for local deposition of diaminobenzidine, a precursor for an electron microscopy stain. APEX2 is a ... 69 (3): 517-532.e11. doi:10.1016/j.molcel.2017.12.020. ISSN 1097-2765. PMID 29395067. Bersuker, Kirill; Peterson, Clark W. H.; ... 3 (1): 38. doi:10.1038/s42003-020-0758-y. ISSN 2399-3642. PMC 6976632. PMID 31969649. Tamura, Tomonori; Takato, Mikiko; Shiono ...
... complex technique using diaminobenzidine (DAB) as a substrate. Tumor Microenvironment: Tissue cytometry is heavily used in ... 6 (3): 256-265. doi:10.4252/wjsc.v6.i3.256. ISSN 1948-0210. PMC 4131268. PMID 25126376. Wong, Tzyy Yue; Chang, Chiung-Hsin; Yu ... 16 (3): 451-460. doi:10.1111/acel.12567. ISSN 1474-9726. PMC 5418204. PMID 28474484. Pillat, Micheli Mainardi; Oliveira- ... 206 (3): 524-530. doi:10.4049/jimmunol.2000433. ISSN 0022-1767. PMC 7978153. PMID 33328214. Munemura, Ryusuke; Maehara, Takashi ...
... diaminobenzidine", issued 1976-03-09, assigned to Celanese Corporation "D.A.B. (Diaminobenzidine)". Chesapeake Bay Division, ... Diaminobenzidine is oxidized by hydrogen peroxide in the presence of hemoglobin to give a dark-brown color. This color change ... Diaminobenzidine, which is commercially available, is prepared by treating 3,3′-dichlorobenzidine with ammonia with a copper ... Diaminobenzidine (DAB) is an organic compound with the formula (C6H3(NH2)2)2. This derivative of benzidine is a precursor to ...
Formula: C12H14N4 / (NH2)2C6H3C6H3(NH2)2. Molecular mass: 214.3 Melting point: 176°C Solubility in water: very poor. Flash ... The recommendations on this Card also apply to 3,3-Diaminobenzidine tetrahydrochloride, CAS-number 7411-49-6. ...
DIAMINOBENZIDINE TABLETS (DAB) - 50 Tabs Biotechnology Grade Each tablet will yield 10ml of Substrate Solution, enough to ... DIAMINOBENZIDINE TABLETS (DAB) - 50 Tabs. Biotechnology Grade Each tablet will yield 10ml of Substrate Solution, enough to ...
Soyez le premier à laisser votre avis sur "(DAB-HCl), 3,3-Diaminobenzidine Tetra Hydrochloride, EM Grade" Annuler la réponse. ... Accueil / MET / Réactifs / (DAB-HCl), 3,3-Diaminobenzidine Tetra Hydrochloride, EM Grade. ...
The chromagen was diaminobenzidine. Negative controls were the BLV-negative control cell line Tb1Lu and adjacent human tissue ... The chromagen was diaminobenzidine. Outcome measurement was a semiquantitative judgment of color density of cells: 1+, light ... Figure 3 shows IS-PCR results obtained by using tax primers for the BLV-negative human sample (no. 143), 1 of the BLV-positive ... Table 3. BLV primers and cycling conditions used for L-PCR, IS-PCR, and preparation of DNA for sequencing for detection of BLV ...
... diaminobenzidine tetrahydrochloride (DAB, Sigma-Aldrich, St. Louis, MO, USA; catalog number D5905) were used as chromophores ( ... diaminobenzidine tetrahydrocholoride; DMSO, dimethylsulfoxide; FB, Fast Blue; FG, Fluorogold; L, lateral; M, medial; PHA-L, ... FIGURE 3. Figure 3. Frontal midline cortical input to PPC. Retrogradely labeled cells in the frontal midline cortex resulting ... 3) Every column of the group being processed was adjusted back to the length value calculated previously for the corresponding ...
50 mg/kg/day via gastric gavage for 3 weeks after weaning). We found that postnatal HF intake aggravated prenatal DEX-induced ... diaminobenzidine (DAB) as the chromogen. The sections were then lightly counterstained with hematoxylin and preserved under ... treatment on systolic blood pressure between 3 and 16 weeks of age. versus control; versus DEX; versus HF; versus DEX + HF. ... Figure 3 Effects of prenatal dexamethasone (DEX), postnatal high-fat (HF) diet, and dimethyl fumarate treatment (DMF) on mRNA ...
Substance Name:4-Nitroaniline; 1-Amino-4-nitrobenzene; 4-Amino-1-nitrobenzene; 4-Aminonitrobenzene; 4-Nitro-1-aminobenzene. CAS Number:100-01-6. ...
Water treatment is required to maintain effluent compliance with standards established by regulatory bodies. Learn about the stages of wastewater and process water treatment along with the corresponding analytical testing methods.
... diaminobenzidine (DAB substrate kit; Vector Laboratories, Burlingame, CA, USA). Nuclei were counterstained by hematoxylin. ... 3Weilin Zhao. ,1Kaoru Midorikawa. ,1Shosuke Kawanishi. ,4Yusuke Hiraku. ,1Shinji Oikawa. ,1Zhe Zhang. ,2Guangwu Huang. ,2and ... Sections of 3 μm thickness were incubated overnight at room temperature with mouse monoclonal anti-CD24 (1 : 100, Abcam). The ... Figure 3 Double immunofluorescence staining of ALDH1A1 and CD44v6 in chronic nasopharyngitis and NPC tissues. The expression of ...
... diaminobenzidine (DAB) detection kit (Dako Cytomation, Denmark) containing secondary antibody. The intensity of PKM2 and ... diaminobenzidine; IRS: immunoreaction score; CCK8: cell Counting Kit-8; PVDF: polyvinylidene fluoride; Co-IP: co- ... 2019;120(3): 317-330.. *Jin S, Yang X, Li J, Yang W, Ma H, Zhang Z p53-targeted lincRNA-p21 acts as a tumor suppressor by ... 2016;213(3): 337-354.. *Yu G, Yu W, Jin G, Xu D, Chen Y, Xia T, et al. PKM2 regulates neural invasion of and predicts poor ...
Cited in 47 publication(s). Independently reviewed in 3 review(s). Immunogen corresponding to… ... Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and ... Lane 3 : Mouse lung tissue lysate at 20 µg. Lane 4 : A431 (Human epidermoid carcinoma cell line) whole cell lysate at 10 µg. ... Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate. Lysates/proteins at 25 µg per lane.. ...
Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and ... Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were ... peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes ( ...
... diaminobenzidine (DAB) horseradish peroxidase biotin-avidin amplification Processing History. unprocessed raw data Data ... Bound antibody was detected using avidin-biotin-peroxidase (Vector Labs) and diaminobenzidine (DAB). Sections were lightly ... Gleason Pattern 3. 50. 0. 50. 2. The table indicates the percent of each cell type with the indicated staining intensity. ...
... diaminobenzidine (DAB) horseradish peroxidase biotin-avidin amplification Processing History. unprocessed raw data Data ... Bound antibody was detected using avidin-biotin-peroxidase (Vector Labs) and diaminobenzidine (DAB). Sections were lightly ...
... diaminobenzidine tetrahydrochloride (DAB, Sigma-Aldrich) and then counterstained with Massons Hemalun and Lithum Carbonate ... 3. Cannabinoid receptors expression in human fetal testis. (A) RNA-seq quantification of transcripts coding for CB1 (CNR1) and ... 3A) and confirmed in RT-qPCR on bulk testes of 7 DW (Fig. 3B). The expression of CB1 and CB2 receptor transcripts in the fetal ... 3C). Dots corresponding to CNR1 transcripts were more abundant than those corresponding to CNR2, in line with our RNA-seq and ...
... diaminobenzidine tetrahydrochloride (DAB)/H2O2 solution. CD3, CD4, and CD8 cells, as well as neutrophils were detected by ... The fatty alcohols consisted of 14:0 (2%), 16:0 (66%), 18:0 (3%), 20:0 (8%), and 22:0 (21%). The sterols of Parauronema acutum ... Unsaturated fatty acids containing one to three double bonds, primarily 18:1(9), 18:2 (9,12), 18:3 (9, 12, 15) and 20:3 (11, 14 ...
... and diaminobenzidine (DAB) as the chromogen. Antigen retrieval was performed by pressurized heating at 125°C in a citrate- ... diaminobenzidine (DAB) chromogen and hematoxylin counterstain. Bar = 60μm (C) and 80μm (D). E. There is moderate positive ... diaminobenzidine (DAB) chromogen and hematoxylin counterstain. Bar = 60μm (C) and 80μm (D). E. There is moderate positive ... diaminobenzidine (DAB) chromogen and hematoxylin counterstain. Bar = 60μm (C) and 80μm (D). E. There is moderate positive ...
3: The epichaperome mediates pathologic changes in proteome connectivity and function in AD.. ... Graph, mean ± SEM, unpaired two-tailed t-test, n = 3. d, e Viability of iPSC-derived neurons cultured for 24 h with Vehicle (V ... 2b, 3e, 7b-d, 8b-d and Supplementary Figs. 4-8 were randomized and investigators were blinded to allocation and outcome ... 3. c, d Affinity-purification of the epichaperome and its AD interactome shows no specific interaction between the epichaperome ...
3: The epichaperome mediates pathologic changes in proteome connectivity and function in AD.. ... Graph, mean ± SEM, unpaired two-tailed t-test, n = 3. d, e Viability of iPSC-derived neurons cultured for 24 h with Vehicle (V ... 2b, 3e, 7b-d, 8b-d and Supplementary Figs. 4-8 were randomized and investigators were blinded to allocation and outcome ... 3. c, d Affinity-purification of the epichaperome and its AD interactome shows no specific interaction between the epichaperome ...
The colour was developed using diaminobenzidine as a chromogen. Finally, this was counter-stained with haematoxylin for 15 ... 3. Huo D, Ikpatt F, and Khramtsov A, et al (2009) Population differences in breast cancer: survey in indigenous African women ... Muibat Haruna1, Adetola Olubunmi Daramola1,2, Nicholas Awodele Awolola1,2, Nahla Mustafa Badr3,4, Adekunbiola Aina Fehintola ... Table 3. Clinicopathological parameters of the tumour phenotypes in relationship to AR expression in TNBC. ...
... diaminobenzidine (DAB) and 0.0005% hydrogen peroxide. Few sections at a time were developed in DAB, and identical developing ... In 3- to 4-month-old male mice, sleeping time was 159 ± 9.4 min in wild-type mice and 218 ± 7 min in Fgf2 knock-outs; in 4-5 ... 3) and of layer V/VI as assessed by latexin (Fig. 5) were not altered. Together, these data suggest that cortical cell ... 3. The disruption of the Fgf2 gene decreases the density of cortical pyramidal cells but not that of calbindin-positive ...
TUNEL positive nuclei were visualized by peroxidase-diaminobenzidine (DAB) reaction. The sections were then counterstained with ... Boffi FM1; Muriel MG1; López RA1; Ferreira V1 Seoane A2,3 And Picco SJ2,3 ... Warming up: 3 min of walk at 1.8 m/sec on a treadmill and 3 min of trot at 3.6 m/sec on a sloped treadmill (5%).. Severe ... 3. Al-Khalidi, U.A.S., and Chaglassian, T.H. The species distribution of xanthine oxidase. Biochem. J., 97: 318-320, 1965. [ ...
This step was followed by incubation with diaminobenzidine and hydrogen peroxide. The sections were counterstained with ... 3B). In the experiment shown in Fig. 3C, the trans-stimulatory effect of estrone sulfate on OAT3-mediated efflux of estrone ... Louis, MO); [3H]acyclovir (333 GBq/mmol), [3H]methotrexate (555 GBq/mmol), and [3H]ochratoxin A (547.6 GBq/mmol) were from ... Figure 3 The transport properties of estrone sulfate via hOAT3. A, the uptake of 50 nM [3H]estrone sulfate in control oocytes ...
Diaminobenzidine tetra hydrochloride), (BD Cat. No. 550880), or Anti-Rat Ig HRP Detection Kit (Cat. No. 551013). ... 3. Place 20 µl of the cell suspension in each well of the adhesion slides and let them adhere at room temperature (RT) for 20 ... 3. Endogenous Biotin Blocking Buffer: Biotin/Avidin Blocking Kit (Vector Laboratories, Cat. No. SP-2001). ... 1. Assemble the Cytospins sample chamber (e.g. Cytospin 3, Shandon, UK or comparable centrifuge), filter card, slide and ...
... diaminobenzidine solution. Slides were then dehydrated and counterstained with Harris hematoxylin. For double immunostaining ... 3. Orimo A, Gupta P, Sgroi DC, et al. Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and ... 3. Orimo A, Gupta P, Sgroi DC, et al. Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and ... Treatment was administered i.v. to groups of six or eight mice and commenced when tumors had reached a size of 100 to 250 mm3. ...
DAB (diaminobenzidine) was used for revelation and hematoxylin for counterstaining. Imaging was carried out on an Axio Imager. ... Generation of Nestin-Cre;Ptpn1lox/lox;Socs-3lox/lox mice.. Socs-3lox mice were obtained from Dr. Yoshimura and Ptpn1lox mice ... A: Body weight curve of double mutants (N-P1/S3-KO; n = 8), Ptpn1 (N-P1-KO; n = 12), and Socs-3 (N-S3-KO; n = 13) single ... A: Body weight curve of double mutants (N-P1/S3-KO; n = 8), Ptpn1 (N-P1-KO; n = 12), and Socs-3 (N-S3-KO; n = 13) single ...
... diaminobenzidine tetrahydrochloride) Liquid System (DakoCytomation) was used to detect immunostaining. Omission of primary ... 3). As EGFR is expressed in the majority of cases of NSCLC, it is an attractive target for the development of therapeutic ... For 3 of those 5 patients, we were able to obtain pairs of tumor specimens before treatment and after the development of ... For the 3 patients who showed intrinsic resistance and 8 of the 13 patients who had shown partial response, tumor specimens ...
... diaminobenzidine (Vector Laboratories, Burlingame, CA, USA).. 2.6. Immunoblot Analysis. A series of immunoblot analyses was ... Dis. 2016, 3, ofw175. [Google Scholar] [CrossRef] [PubMed]. *Lo, M.K.; Shi, P.Y.; Chen, Y.L.; Flint, M.; Spiropoulou, C.F. In ... Figure 3. Profiling of viral structural protein expression. BHK-21 cells were mock-transfected or transfected with 2 μg of the ... Figure 3. Profiling of viral structural protein expression. BHK-21 cells were mock-transfected or transfected with 2 μg of the ...
... with diaminobenzidine as the chromagen. Positive controls were lung sections from rats that had been instilled with ... 2 After blocking endogenous peroxidase in 3% H202:Methanol (1:1), slides were placed in 10% BSA for 3Q min, then incubated ...
  • The recommendations on this Card also apply to 3,3'-Diaminobenzidine tetrahydrochloride, CAS-number 7411-49-6. (inchem.org)
  • Bound antibody was detected using avidin-biotin-peroxidase (Vector Labs) and diaminobenzidine (DAB). (cellimagelibrary.org)
  • 2 After blocking endogenous peroxidase in 3% H202:Methanol (1:1), slides were placed in 10% BSA for 3Q min, then incubated overnight in the primary antibody (Monoclonal anti-iNOS, Transduction Laboratories, N32020, 1 :50 dilution). (cdc.gov)
  • The DAKO LSAB-2 kit for rat specimens (K0609) was used to label the antibody, with diaminobenzidine as the chromagen. (cdc.gov)
  • Here, we showed that hepatocyte growth factor (HGF), a ligand of MET oncoprotein, induces gefitinib resistance of lung adenocarcinoma cells with EGFR -activating mutations by restoring the phosphatidylinositol 3-kinase/Akt signaling pathway via phosphorylation of MET, but not EGFR or ErbB3. (aacrjournals.org)
  • There is increasing interest in the role played by the innate and adaptive immune systems in the regulation of tumour development and progression 2 , 3 and it is anticipated that a better understanding of the molecular and cellular immunology of NSCLC will lead to the identification of novel targets for immunotherapy. (ersjournals.com)
  • Although Barrett's adenocarcinoma is the most rapidly increasing cancer in Western countries, esophageal squamous cell carcinoma (ESCC) remains dominant in East Asia ( 3 ). (spandidos-publications.com)
  • Esophageal Squamous Cell Carcinoma (ESCC) and esophageal adenocarcinoma[ 2 , 3 ]. (ijpsonline.com)
  • 3,3′-Diaminobenzidine (DAB) is an organic compound with the formula (C6H3(NH2)2)2. (wikipedia.org)
  • A cDNA encoding a multispecific organic anion transporter 3 (hOAT3) was isolated from a human kidney cDNA library. (aspetjournals.org)
  • For example, even though glucocorticoids are recommended to women at risk of preterm birth to accelerate fetal lung maturation [ 2 ], a growing body of evidence indicates that prenatal glucocorticoid exposure induces a variety of adult diseases, including hypertension [ 3 - 5 ]. (hindawi.com)
  • This signal together with the T Cell Receptor (TCR) induces the expression of GATA-3. (bvsalud.org)
  • During inflammation, reactive oxygen species (ROS) and reactive nitrogen species (RNS) are generated from inflammatory cells and are considered to play key roles in carcinogenesis [ 3 ]. (hindawi.com)
  • These results show that the biological roles of SOCS-3 and PTP-1B do not fully overlap and that targeting both factors might improve therapeutic effects of their inhibition in obesity and type 2 diabetes. (diabetesjournals.org)
  • Usually, protective and destructive roles are assigned to the Th1 and Th17 3,9-11 cells, while Th2 and Treg cells are more involved in processes that reduce the destruction of the periodontium 10 . (bvsalud.org)
  • Most hereditary colorectal cancers are and hMSH6 ) in colorectal cancer stained with haematoxylin, dehydrated attributable to 2 recognized syndromes: cases operated at our institution over in ascending concentrations of alcohol familial adenomatous polyposis and a 3-year period. (who.int)
  • Inflammation-associated tissue injury may activate stem/progenitor cells, and mutagenic stimuli from inflammation can accumulate multiple mutations and epigenetic changes in stem/progenitor cells [ 3 , 10 ]. (hindawi.com)
  • We generated mice with combined inactivation of the genes coding for SOCS-3 and PTP-1B in brain cells, examined their sensitivity to hormone action, and analyzed the contribution of each gene to the resulting phenotype. (diabetesjournals.org)
  • Urothelial carcinoma (UC) as a common genitourinary cancer, accounting for 4.5% of all new cancer cases and 3% of cancer-related deaths in 2020, is the sixth most common type of cancer [ 1 ]. (biomedcentral.com)
  • In the United States, ≈38% of beef herds, 84% of all dairy herds, and 100% of large-scale dairy operation herds are infected ( 3 , 4 ). (cdc.gov)
  • These findings suggest that the response to anti-PD-1 hinges on pre-existing anti-tumor immune response and that anti-PD-1 acts to free the CD8 T cells from inhibition to exert their anti-tumor activities [ 3 ]. (biomedcentral.com)
  • GATA-3 and IFN-γ R1 subunit expressions were evaluated during different stages of induced periodontal disease (early and advanced) and these data were correlated with the severity of periodontal destruction, which was determined clinically and histologically. (bvsalud.org)
  • The interleukin-1 (IL-1) family is a growing group of cytokines, consisting of at least 11 members, and the balance between pro- and anti-inflammatory cytokines is crucial in the pathogenesis of many human diseases ( 3 ). (frontiersin.org)
  • Results: Light microscopy analysis revealed a decreased expression of GATA-3-positive cells when bone loss progressed. (bvsalud.org)
  • TNBC accounts for 170,000 cases of the global breast cancer burden and has been associated with disparities in racial incidence [ 3 ]. (ecancer.org)
  • Genetics the status of 2 major microsatellite in- Diamino benzidine was used as a chro- may play a role in up to 30% of cases [3]. (who.int)
  • Estrone sulfate did not show any trans -stimulatory effects on either influx or efflux of [ 3 H]estrone sulfate via hOAT3. (aspetjournals.org)
  • The function is performed by identifying 3′-UTRs (untranslated regions) of target mRNAs with conserved complementarities to the seed (nucleotides 2-7) of the miRNA. (spandidos-publications.com)
  • Four thoroughbreds, ranging in aged from 3 to 4 years old were used for this study. (scielo.org.ar)
  • Nonetheless, combined inactivation of SOCS-3 and PTP-1B in brain revealed additive effects on several parameters, including partial resistance to DIO and associated glucose intolerance. (diabetesjournals.org)
  • OLR tend to be unilateral and erosive, with a slightly more diffuse lymphocytic infiltrate with increased eosinophils, plasmocytes, and cytoid bodies than OLP, although it is common to find similar histological characteristics in both entities [ 1 - 3 ]. (ecancer.org)
  • 14. Use of progesterone-3(O-carboxymethyl oxime)-horseradish peroxidase in a sensitive microtitre-plate EIA and its application to a visual membrane EIA of progesterone. (nih.gov)
  • 12. A simple enhancement method for the silver-gold-intensified diaminobenzidine reaction in the light microscopic immunoperoxidase technique. (nih.gov)
  • Postnuclear supernatants of homogenized cells were incubated with 3,3'-diaminobenzidine (DAB) and H2O2. (rupress.org)
  • DAB (3, 3 -diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications. (selectscience.net)
  • 3,3′-Diaminobenzidine (DAB) is an organic compound with the formula (C6H3(NH2)2)2. (wikipedia.org)
  • PDD is characterized by severe lymphoplasmacytic inflammation in peripheral, central, and autonomic nervous tissues ( 3 - 5 ). (cdc.gov)
  • 20. Stability and solubility of 3,3'-diaminobenzidine (DAB). (nih.gov)
  • The pathogenesis of VA is still poorly understood, despite recent findings pointing to mutations in genes such as phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha ( PIK3CA ) ( 4 ) and angiopoietin receptor ( TIE2 ) ( 5 ) as a possible underlying cause. (iiarjournals.org)
  • GATA-3 and IFN-γ R1 subunit expressions were evaluated during different stages of induced periodontal disease (early and advanced) and these data were correlated with the severity of periodontal destruction, which was determined clinically and histologically. (bvsalud.org)
  • FeLV-B is caused by recombination in the env gene between FeLV-A and the endogenous FeLV genome, and FeLV-C is derived from mutations in the env gene of FeLV-A [ 3 , 4 ]. (biomedcentral.com)
  • RNA derived from AVM (n=4) and normal skin control (n=3) tissues was evaluated by RNA sequencing. (iiarjournals.org)
  • Moreover, the survival rate is only 12-15 months in patients with cancers of an advanced stage undergoing classical cisplatin-based systemic chemotherapy ( 3 ). (spandidos-publications.com)
  • The human anti-PD-1 antibodies, nivolumab and pembrolizumab, have shown significant survival benefits over docetaxel in phase 3 trials of refractory NSCLC patients ( 5 - 7 ). (iiarjournals.org)
  • It is a multi-ligand receptor and regarded as a central mediator in chronic inflammatory and immune responses 3 . (nature.com)
  • The current immunomodulating drugs for MS, such as interferon β , glatiramer acetate, and natalizumab, prevent inflammatory damage to the CNS, which do not effectively prevent the progressive clinical neurological decline most likely because they lack a direct action on CNS axons and myelin [ 3 ]. (hindawi.com)
  • 3 Division of Clinical Immunology, Karolinska Institutet at Karolinska University Hospital Huddinge, Stockholm, Sweden. (jci.org)
  • 3. Department of Biological Sciences, Faculty of Sciences and Letters, UNESP - Univ. (jcancer.org)
  • these impairments approximate 3-5 years of accelerated cognitive loss ( Chen and Schwartz 2009 ). (nih.gov)
  • 1 Division of Pulmonary and Critical Care Medicine, 2 Division of Nutrition, and 3 Departments of Pathology and Pediatrics, David Geffen School of Medicine at University of California-Los Angeles, Los Angeles, California, USA. (jci.org)
  • Diabetes is also associated with raised AGE levels and subsequent up regulation of inflammation occurs as a consequence of AGE activation of RAGE 3 . (nature.com)
  • This study examined adipose and muscle inflammatory markers after treatment of humans with FOs and measured the effects of ω-3 fatty acids on adipocytes and macrophages in vitro. (diabetesjournals.org)
  • 3 The development of SOS after HSCT is associated with injury to sinusoidal endothelial cells (ECs) and hepatocytes via a variety of factors, including a hepatotoxic conditioning regimen, immunosuppressive treatments with calcineurin inhibitors, and lipopolysaccharide (LPS) released by gram-negative bacteria. (haematologica.org)
  • The cells were subsequently harvested, washed and recultured with recombinant human IL-2 and recombinant human IL-4 for an additional 3 days. (bdbiosciences.com)
  • In addition, forced overexpression of CYP2J2, and CYP BM3F87V or addition of epoxyeicosatrienoic acids (EET) in cultured carcinoma cell lines in vitro markedly accelerated proliferation by analyses of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, cell accounts, and cell cycle analysis, and protected carcinoma cells from apoptosis induced by tumor necrosis factor α (TNF-α) in cultures. (aacrjournals.org)
  • Mitochondria are the powerhouse of eukaryotic cells and are responsible for regulating energy metabolism, respiration and cell apoptosis [ 3 ]. (oncotarget.com)
  • Results: Light microscopy analysis revealed a decreased expression of GATA-3-positive cells when bone loss progressed. (bvsalud.org)
  • Usually, protective and destructive roles are assigned to the Th1 and Th17 3,9-11 cells, while Th2 and Treg cells are more involved in processes that reduce the destruction of the periodontium 10 . (bvsalud.org)
  • Insulin-resistant, nondiabetic subjects were treated with Omega-3-Acid Ethyl Esters (4 g/day) or placebo for 12 weeks. (diabetesjournals.org)
  • Other significantly down-regulated genes included those coding osteocalcin, bone morphogenetic proteins (-3, -4 and -7), collagens (-1a1, -14a1) or Phex . (frontiersin.org)
  • After exposure to nPM, the OE and OB had rapid increases of 4-hydroxy-2-nonenal (4-HNE) and 3-nitrotyrosine (3-NT) protein adducts, whereas the cerebral cortex and cerebellum did not respond at any time. (nih.gov)
  • While Mel therapy was unable to reduce Her-4 and phosphoinositide 3-kinase (PI3K) levels, it was able to suppress the OC-related increase in the levels of the Her-2, p38 mitogen-activated protein kinases (p38 MAPK), protein kinase B (phospho-AKT), and mammalian target of rapamycin (mTOR). (jcancer.org)
  • When cultured in vitro they can undergo only a finite number of cell divisions before entering a nondividing state, referred to as replicative senescence, 1 , 2 , 3 which has been suggested to potentially contribute to the aging process and the development of age-related diseases. (nature.com)
  • 3. Chromogen-based immunohistochemical method for elucidation of the coexpression of two antigens using antibodies from the same species. (nih.gov)
  • IHC staining was done using the Avidin-Biotin complex (ABC) method, diaminobenzidine serving as a chromogen. (ijpmonline.org)
  • After activation, caspase-8 processes down-stream executor caspases (-3, -6, -7), which cleave their substrates and ensure apoptotic progression ( Opdenbosch and Lamkanfi, 2019 ). (frontiersin.org)
  • Bacteria are capable of damaging directly the host tissue, but it can also be destroyed by inflammatory processes resulting from this aggression as consequence of the exaggerated immunoinflammatory and proteolytic processes 2 that activate osteoclasts 3 . (bvsalud.org)
  • Levels are lower in diabetic patients with complications versus those without 3 . (nature.com)
  • Vascular anomalies (VA) of the head and neck area are a highly heterogeneous group of rare vascular diseases ( 1 - 3 ). (iiarjournals.org)
  • 0.05) with the use of Pro+Glu 2 and Pro+Glu 3 than in the control. (bvsalud.org)
  • e) 120 dB startle amplitude (Vmax) over blocks of 5 trials and (f) percent inhibition of 120 dB response from pre-pulse stimulus intensities of 3, 6, 12, or 15 dB above background. (nih.gov)
  • Quantitative PCR confirmed the presence of the virus in brain, proventriculus, and adrenal gland in 3 birds with PDD but not in 4 unaffected birds. (cdc.gov)
  • Resistance to chemotherapy is a major factor limiting long-term treatment against OC [ 2 ], and paclitaxel resistance has been recognized to induce protumor activities [ 3 , 4 ]. (jcancer.org)