2S Albumins, Plant: A major class of water-soluble seed storage proteins. Many proteins from this class are major PLANT ALLERGENS.Albumins: Water-soluble proteins found in egg whites, blood, lymph, and other tissues and fluids. They coagulate upon heating.Antigens, Plant: Substances found in PLANTS that have antigenic activity.Plants, Genetically Modified: PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.Serum Albumin: A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.Sesamum: A plant genus of the family PEDALIACEAE that is the source of the edible seed and SESAME OIL.Plant Proteins: Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.Bertholletia: A plant genus of the family Lecythidaceae which is the source of edible Brazil nuts.Nuts: Botanically, a type of single-seeded fruit in which the pericarp enclosing the seed is a hard woody shell. In common usage the term is used loosely for any hard, oil-rich kernel. Of those commonly eaten, only hazel, filbert, and chestnut are strictly nuts. Walnuts, pecans, almonds, and coconuts are really drupes. Brazil nuts, pistachios, macadamias, and cashews are really seeds with a hard shell derived from the testa rather than the pericarp.Plant Leaves: Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)Seeds: The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield.Plants, Toxic: Plants or plant parts which are harmful to man or other animals.Plant Roots: The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)Genes, Plant: The functional hereditary units of PLANTS.Serum Albumin, Bovine: Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)Arachis hypogaea: A plant species of the family FABACEAE that yields edible seeds, the familiar peanuts, which contain protein, oil and lectins.Plant Extracts: Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard.Plant Shoots: New immature growth of a plant including stem, leaves, tips of branches, and SEEDLINGS.Protein PrecursorsPlants, Medicinal: Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals.DNA, Plant: Deoxyribonucleic acid that makes up the genetic material of plants.Plant Development: Processes orchestrated or driven by a plethora of genes, plant hormones, and inherent biological timing mechanisms facilitated by secondary molecules, which result in the systematic transformation of plants and plant parts, from one stage of maturity to another.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Plant Cells: Basic functional unit of plants.Plant Stems: Parts of plants that usually grow vertically upwards towards the light and support the leaves, buds, and reproductive structures. (From Concise Dictionary of Biology, 1990)Genome, Plant: The genetic complement of a plant (PLANTS) as represented in its DNA.Arabidopsis: A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Plants, Edible: An organism of the vegetable kingdom suitable by nature for use as a food, especially by human beings. Not all parts of any given plant are edible but all parts of edible plants have been known to figure as raw or cooked food: leaves, roots, tubers, stems, seeds, buds, fruits, and flowers. The most commonly edible parts of plants are FRUIT, usually sweet, fleshy, and succulent. Most edible plants are commonly cultivated for their nutritional value and are referred to as VEGETABLES.Plant Structures: The parts of plants, including SEEDS.Plant Growth Regulators: Any of the hormones produced naturally in plants and active in controlling growth and other functions. There are three primary classes: auxins, cytokinins, and gibberellins.
(1/59) Co-introduction of an antisense gene for an endogenous seed storage protein can increase expression of a transgene in Arabidopsis thaliana seeds.

We have investigated whether the expression in Arabidopsis thaliana seeds of a transgene (the Phaseolus vulgaris arcelin (arc)5-I gene) could be enhanced by the simultaneous introduction of an antisense gene for an endogenous seed storage protein (2S albumin). Seeds of plants transformed with both the arc5-I gene and a 2S albumin antisense gene contained reduced amounts of 2S albumins and increased arcelin-5 (Arc5) accumulation levels compared to lines harboring the arc5-I gene only. Arc5 production could be enhanced to more than 24% of the total seed protein content, suggesting that antisense technology could be of great utility to favor high expression of transgenes.  (+info)

(2/59) Direct kinetic evidence for folding via a highly compact, misfolded state.

The 2 S seed storage protein, sunflower albumin 8 (SFA-8), contains an unusually high proportion of hydrophobic residues including 16 methionines (some of which may form a surface hydrophobic patch) in a disulfide cross-linked, alpha-helical structure. Circular dichroism and fluorescence spectroscopy show that SFA-8 is highly stable to denaturation by heating or chaotropic agents, the latter resulting in a reversible two-state unfolding transition. The small m(U) (-4.7 M(-1) at 10 degrees C) and DeltaC(p) (-0.95 kcal mol(-1) K(-1)) values indicate that relatively little nonpolar surface of the protein is exposed during unfolding. Commensurate with the unusual distribution of hydrophobic residues, stopped-flow fluorescence data show that the folding pathway of SFA-8 is highly atypical, in that the initial product of the rapid collapse phase of folding is a compact nonnative state (or collection of nonnative states) that must unfold before acquiring the native conformation. The inhibited folding reaction of SFA-8, in which the misfolded state (m(M) = -0.95 M(-1) at 10 degrees C) is more compact than the transition state for folding (m(T) = -2.5 M(-1) at 10 degrees C), provides direct kinetic evidence for the transient misfolding of a protein.  (+info)

(3/59) GerN, an antiporter homologue important in germination of Bacillus cereus endospores.

A homologue of the grmA spore germination gene of Bacillus megaterium and of a NaH-antiporter gene (napA) of Enterococcus hirae has been identified in Bacillus cereus 569 (ATCC 10876). The putative protein product has 58 and 43% amino acid identity with GrmA and NapA, respectively. Insertional inactivation of this B. cereus gene, named gerN, did not affect vegetative growth or sporulation. The null mutant spores were 30-fold slower to germinate in inosine (5 mM) but germinated almost normally in response to L-alanine (10 mM). The null mutant spores germinated after several hours with inosine as the sole germinant, but germination was asynchronous and the normal order of germination events was perturbed. At a suboptimal germinant concentration (50 microM), inosine germination was completely blocked in the mutant, while the rate of germination in 50 microM L-alanine was reduced to one-third of that of the wild type. The requirement for GerN function in the response to a particular germinant suggests that a germination receptor may have a specifically associated antiporter, which is required at the initiation of germination and which, in the case of the inosine receptor, is GerN. Since germination in suboptimal concentrations of L-alanine shows a delay, additional germination transporters may be required for optimal response at low germinant concentrations.  (+info)

(4/59) Calcium-mediated association of a putative vacuolar sorting receptor PV72 with a propeptide of 2S albumin.

PV72, a type I membrane protein with three epidermal-growth factor (EGF)-like motifs, was found to be localized on the membranes of the precursor-accumulating (PAC) vesicles that accumulated precursors of various seed storage proteins. To clarify the function of PV72 as a sorting receptor, we expressed four modified PV72s and analyzed their ability to bind the internal propeptide (the 2S-I peptide) of pro2S albumin by affinity chromatography and surface plasmon resonance. The recombinant PV72 specifically bound to the 2S-I peptide with a K(D) value of 0.2 microm, which was low enough for it to function as a receptor. The EGF-like motifs modulated the Ca(2+)-dependent conformational change of PV72 to form a functional pocket for the ligand binding. The binding of Ca(2+) stabilizes the receptor-ligand complex even at pH 4.0. The association and dissociation of PV72 with the ligand is modulated by the Ca(2+) concentration (EC(50) value = 40 microm) rather than the environmental pH. Overall results suggest that Ca(2+) regulates the vacuolar sorting mechanism in higher plants.  (+info)

(5/59) Recombinant pronapin precursor produced in Pichia pastoris displays structural and immunologic equivalent properties to its mature product isolated from rapeseed.

2S albumin storage proteins from rapeseed (Brassica napus), called napins, consist of two different polypeptide chains linked by disulphide bridges, which are derived by proteolytic cleavage from a single precursor. The precursor form of the napin BnIb (proBnIb) has been cloned using a PCR strategy and sequenced. The amino-acid sequence deduced from the clone includes 31 residues of the small chain and 75 of the large chain, which are connected by the peptide Ser-Glu-Asn. Expression of the cDNA encoding proBnIb has been carried out in the methylotrophic yeast Pichia pastoris. The induced protein was secreted to the extracellular medium at a yield of 80 mg.L(-1) of culture and was purified by means of size-exclusion chromatography and reverse phase-HPLC. Recombinant proBnIb appeared properly folded as its molecular and spectroscopic properties were equivalent to those of the mature heterodimeric protein. As 2S albumin storage proteins from Brassicaceae have been shown to be type I allergy inducers, the immunological activity of the recombinant proBnIb was analysed as a measure of its structural integrity. The immunological properties of the recombinant precursor and the natural napin were indistinguishable by immunoblotting and ELISA inhibition using polyclonal antisera and sera of patients allergic to mustard and rapeseed. In conclusion, the recombinant expression of napin precursors in P. pastoris has been shown to be a successful method for high yield production of homogeneous and properly folded proteins whose polymorphism and complex maturation process limited hitherto their availability.  (+info)

(6/59) Protein structure plays a critical role in peanut allergen stability and may determine immunodominant IgE-binding epitopes.

Hypersensitivity to peanuts is a reaction mediated by IgE Abs in response to several peanut protein allergens. Among these allergenic proteins, Ara h 2 is one of the most commonly recognized allergens. Ara h 2 is a 17-kDa protein that has eight cysteine residues that could form up to four disulfide bonds. Circular dichroism studies showed substantial changes in the secondary and tertiary structures of the reduced Ara h 2 as compared with the native protein. Upon treatment with trypsin, chymotrypsin, or pepsin, a number of relatively large fragments are produced that are resistant to further enzymatic digestion. These resistant Ara h 2 peptide fragments contain intact IgE-binding epitopes and several potential enzyme cut sites that are protected from the enzymes by the compact structure of the protein. The enzyme-treated allergen remains essentially intact despite the action of proteases until the fragments are dissociated when the disulfide linkages are reduced. Amino acid sequence analysis of the resistant protein fragments indicates that they contain most of the immunodominant IgE-binding epitopes. These results provide a link between allergen structure and the immunodominant IgE-binding epitopes within a population of food-allergic individuals.  (+info)

(7/59) Engineered recombinant peanut protein and heat-killed Listeria monocytogenes coadministration protects against peanut-induced anaphylaxis in a murine model.

Peanut allergy (PNA) is the major cause of fatal and near-fatal anaphylactic reactions to foods. Traditional immunotherapy using peanut (PN) protein is not an option for PNA therapy because of the high incidence of adverse reactions. We investigated the effects of s.c. injections of engineered (modified) recombinant PN proteins and heat-killed Listeria monocytogenes (HKLM) as an adjuvant on anaphylactic reactions in a mouse model of PN allergy. PN-allergic C3H/HeJ mice were treated s.c. with a mixture of the three major PN allergens and HKLM (modified (m)Ara h 1-3 plus HKLM). The effects on anaphylactic reactions following PN challenge and the association with Ab levels and cytokine profiles were determined. Although all mice in the sham-treated groups exhibited anaphylactic symptoms with a median symptom score of 3, only 31% of mice in the mAra h 1-3 plus HKLM group developed mild anaphylaxis, with a low median symptom score of 0.5. Alterations in core body temperature, bronchial constriction, plasma histamine, and PN-specific IgE levels were all significantly reduced. This protective effect was markedly more potent than in the mAra h 1-3 protein alone-treated group. HKLM alone did not have any protective effect. Reduced IL-5 and IL-13, and increased IFN-gamma levels were observed only in splenocytes cultures from mAra h 1-3 plus HKLM-treated mice. These results show that immunotherapy with modified PN proteins and HKLM is effective for treating PN allergy in this model, and may be a potential approach for treating PNA.  (+info)

(8/59) A plant-based allergy vaccine suppresses experimental asthma via an IFN-gamma and CD4+CD45RBlow T cell-dependent mechanism.

Allergic asthma is currently considered a chronic airway inflammatory disorder associated with the presence of activated CD4(+) Th2-type lymphocytes, eosinophils, and mast cells. Interestingly, therapeutic strategies based on immune deviation and suppression have been shown to successfully attenuate the development of the asthma phenotype. In this investigation, we have for the first time used a genetically modified (GM) plant, narrow leaf lupin (Lupinus angustifolius L.), expressing a gene for a potential allergen (sunflower seed albumin) (SSA-lupin) to examine whether a GM plant/food-based vaccine strategy can be used to suppress the development of experimental asthma. We show that oral consumption of SSA-lupin promoted the induction of an Ag-specific IgG2a Ab response. Furthermore, we demonstrate that the plant-based vaccine attenuated the induction of delayed-type hypersensitivity responses and pathological features of experimental asthma (mucus hypersecretion, eosinophilic inflammation, and enhanced bronchial reactivity (airways hyperreactivity). The suppression of experimental asthma by SSA-lupin was associated with the production of CD4(+) T cell-derived IFN-gamma and IL-10. Furthermore, we show that the specific inhibition of experimental asthma was mediated via CD4(+)CD45RB(low) regulatory T cells and IFN-gamma. Thus, our data demonstrate that a GM plant-based vaccine can promote a protective immune response and attenuate experimental asthma, suggesting that plant-based vaccines may be potentially therapeutic for the protection against allergic diseases.  (+info)

*  Cupin superfamily
... seed storage proteins of grasses and cereals belong to the eponymous prolamin superfamily which also includes plant albumins(2S ... "Germins and germin-like proteins: Plant do-all proteins. But what do they do exactly?". Plant Physiology and Biochemistry. 39: ... but involved in plant responses to biotic and abiotic stress. "G-OXOs and GLPs are plant do-all proteins." Germin of the "true ... 400 plant species are susceptible including stone fruits. Thomas Burr Osborne at the end of the 19th century was the first ...
*  Phytepsin
... bonds in 2S albumin from plant seeds This enzyme is present in barley grain and other plants. Runeberg-Roos, P.; Törmäkangas, K ... A plant aspartic proteinase resembling mammalian cathepsin D". Eur. J. Biochem. 202 (3): 1021-1027. doi:10.1111/j.1432- ... "Structure and possible function of aspartic proteinases in barley and other plants". Adv. Exp. Med. Biol. Advances in ...
*  Plant lipid transfer proteins
Bruix M, Santoro J, Rico M, Gimenez-Gallego G, Pantoja-Uceda D (2003). "Solution structure of RicC3, a 2S albumin storage ... Plant lipid transfer proteins, also known as plant LTPs or PLTPs, are a group of highly-conserved proteins of about 9kDa found ... Plant lipid transfer proteins share the same structural domain with seed storage proteins and trypsin-alpha amylase inhibitors ... LTPs in plants may be involved in: cutin biosynthesis surface wax formation mitochondrial growth pathogen defense reactions ...
*  Soy protein
Soybeans contain a small but newly very significant 2S Albumin storage protein. Legume proteins, such as soy and pulses, belong ... However, Julian's plant must have also been the source of the "soy protein isolate" which Ford's Robert Boyer and Frank Calvert ... "2S Albumin Storage Proteins: What Makes them Food Allergens?". Open Biochem J. 2: 16-28. 2008. doi:10.2174/1874091X00802010016 ... The plant's eventual daily output of 40 tons of soy protein isolate made the Soya Products Division into Glidden's most ...
*  Lunasin
Ben de Lumen at the University of California-Berkeley identified the peptide as a subunit of the cotyledon-specific 2S albumin ... AF005030)" in "The Electronic Plant Gene Register". Plant Physiology. 114 (4): 1567-9. 1997. doi:10.1104/pp.114.4.1567. PMC ...
*  Gaseous signaling molecules
Ethylene serves as a hormone in plants. It acts at trace levels throughout the life of the plant by stimulating or regulating ... Additionally, H 2S reacts with intracellular S-nitrosothiols to form the smallest S-nitrosothiol (HSNO), and a role of hydrogen ... Sampath V, Zhao XJ, Caughey WS (Apr 27, 2001). "Anesthetic-like interactions of nitric oxide with albumin and hemeproteins. A ... It wasn't until 1934 that Gane reported that plants synthesize ethylene. In 1935, Crocker proposed that ethylene was the plant ...
DiVA - Search result  DiVA - Search result
The allergenicity of 2S plant albumins2011In: Clinical and Experimental Allergy, ISSN 0954-7894, E-ISSN 1365-2222, Vol. 41, no ... Mustard plants belong to the Brassicaceae/Cruciferae family. Mustard is present as an ingredient in different foods, sauces and ...
more infohttp://www.diva-portal.org/smash/resultList.jsf?searchType=SUBJECT&language=en&onlyFullText=false&af=%5B%5D&aq=%5B%5B%7B%22categoryId%22%3A%2211553%22%7D%5D%5D
DiVA - Search result  DiVA - Search result
The allergenicity of 2S plant albumins2011In: Clinical and Experimental Allergy, ISSN 0954-7894, E-ISSN 1365-2222, Vol. 41, no ...
more infohttp://www.diva-portal.org/smash/resultList.jsf?af=%5B%5D&aq=%5B%5B%7B%22journalId%22%3A%228710%22%7D%5D%5D&aqe=%5B%5D&aq2=%5B%5B%5D%5D&language=en&query=
DiVA - Search result  DiVA - Search result
The allergenicity of 2S plant albumins2011In: Clinical and Experimental Allergy, ISSN 0954-7894, E-ISSN 1365-2222, Vol. 41, no ... The ins and outs of stable isotopes in plants.2007In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 174, no 3, 473- ... Ber e 1 was also one of the first food allergens to be unintentionally transferred from one plant to another and was involved ... A comparison of the D isotopomer abundances of soluble sugars of annual plants and of trees, and of tree-ring cellulose shows ...
more infohttp://umu.diva-portal.org/smash/resultList.jsf?af=%5B%5D&aq=%5B%5B%7B%22organisationId%22%3A%22791%22%7D%5D%5D&aqe=%5B%5D&aq2=%5B%5B%5D%5D&language=en&query=
DiVA - Search result  DiVA - Search result
The allergenicity of 2S plant albumins2011In: Clinical and Experimental Allergy, ISSN 0954-7894, E-ISSN 1365-2222, Vol. 41, no ... The ins and outs of stable isotopes in plants.2007In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 174, no 3, p. 473 ... Ber e 1 was also one of the first food allergens to be unintentionally transferred from one plant to another and was involved ... A comparison of the D isotopomer abundances of soluble sugars of annual plants and of trees, and of tree-ring cellulose shows ...
more infohttp://umu.diva-portal.org/smash/resultList.jsf?searchType=ORGANISATION&language=en&onlyFullText=false&af=%5B%5D&aq=%5B%5B%7B%22organisationId%22%3A%22791%22%7D%5D%5D
Cupin superfamily - Wikipedia  Cupin superfamily - Wikipedia
... seed storage proteins of grasses and cereals belong to the eponymous prolamin superfamily which also includes plant albumins(2S ... "Germins and germin-like proteins: Plant do-all proteins. But what do they do exactly?". Plant Physiology and Biochemistry. 39: ... but involved in plant responses to biotic and abiotic stress. "G-OXOs and GLPs are plant do-all proteins." Germin of the "true ... 400 plant species are susceptible including stone fruits. Thomas Burr Osborne at the end of the 19th century was the first ...
more infohttps://en.wikipedia.org/wiki/Cupin_superfamily
Table of Contents - August 01, 2006, 177 (3) | The Journal of Immunology  Table of Contents - August 01, 2006, 177 (3) | The Journal of Immunology
Differential Polarization of Immune Responses by Plant 2S Seed Albumins, Ber e 1, and SFA8 Dorothy E. Kean, Helen S. Goodridge ...
more infohttp://www.jimmunol.org/content/177/3
Peptides (v.30, #7) | www.chemweb.com  Peptides (v.30, #7) | www.chemweb.com
Recombinant rJug r 1 adopts the canonical α-helical fold of plant 2S albumins as checked on CD spectra. Four IgE-binding ... Jug r 1, the 2S albumin allergen from walnut, was isolated from ripe nuts as a native allergen and expressed in Escherichia ... Expression of Jug r 1, the 2S albumin allergen from walnut (Juglans regia), as a correctly folded and functional recombinant ...
more infohttps://chemweb.com/articles/01969781/00300007
Oral immunization with transgenic plants - The Texas A&M University System  Oral immunization with transgenic plants - The Texas A&M University System
... the present invention are produced in transgenic plants and then administered through the consumption of the transgenic plant. ... In such plants such as but not limited to Brassica napus (canola), expression vectors including the 2S albumin promoter and ... This term includes, without limitation, whole plants, plant cells, plant organs, plant seeds, protoplasts, callus, cell ... Ann De Clercq et al., Expression and Processing of an Arabidopsis 2S Albumin in Transgenic Tobacco, Plant Physiol. (1990) vol. ...
more infohttp://www.freepatentsonline.com/6395964.html
Phytepsin - Wikipedia  Phytepsin - Wikipedia
... bonds in 2S albumin from plant seeds This enzyme is present in barley grain and other plants. Runeberg-Roos, P.; Törmäkangas, K ... A plant aspartic proteinase resembling mammalian cathepsin D". Eur. J. Biochem. 202 (3): 1021-1027. doi:10.1111/j.1432- ... "Structure and possible function of aspartic proteinases in barley and other plants". Adv. Exp. Med. Biol. Advances in ...
more infohttps://en.wikipedia.org/wiki/Phytepsin
Tomato  - Phadia - Setting the Standard - Phadia.com  Tomato - Phadia - Setting the Standard - Phadia.com
The sequence of Lec2SA was similar to that of 2S albumins from different plants, such as Brazil nut and castor beans (26). The ... The study concluded that treatment with plant hormones induced a more intense cutaneous response (25).. A 2S storage albumin, ... Oguri S, Kamoshida M, Nagata Y, Momonoki YS, Kamimura H. Characterization and sequence of tomato 2S seed albumin: a storage ... Plant Biotech J 2006;4(2):231-42 *Lorenz Y, Enrique E, Lequynh L, Fotisch K, Retzek M, Biemelt S, Sonnewald U, Vieths S, ...
more infohttp://www.phadia.com/en/Products/Allergy-testing-products/ImmunoCAP-Allergen-Information/Food-of-Plant-Origin/Vegetables/Tomato-/
Evidence for the rapid expansion of microRNA-mediated regulation in early land plant evolution | BMC Plant Biology | Full Text  Evidence for the rapid expansion of microRNA-mediated regulation in early land plant evolution | BMC Plant Biology | Full Text
Thus, the number and function of miRNAs must have significantly expanded during the evolution of early land plants. As we have ... Frequently, not only single miRNAs but whole families of closely related miRNAs have been found in animals and plants. Some ... the coupled maturation of two miRNAs from a shared precursor has not been previously identified in plants. ... families are widely conserved among different plant taxa. Hence, it is evident that these conserved miRNAs are of ancient ...
more infohttps://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-7-13
Transport of Storage Proteins to Protein Storage Vacuoles Is Mediated by Large Precursor-Accumulating Vesicles | Plant Cell  Transport of Storage Proteins to Protein Storage Vacuoles Is Mediated by Large Precursor-Accumulating Vesicles | Plant Cell
Immunocytochemical analysis with 2S albumin-specific antibodies demonstrated that the ER aggregates contained 2S albumin, as ... 1993a). Vesicle transport and processing of the precursor to 2S albumin in pumpkin. Plant J. 4, 793-800. ... 1996). Compartmentation of proteins in the endomembrane system of plant cells. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47, ... G indicates 11S globulin subunits; 2S, 2S albumin; and 51kD, the 51-kD protein. ...
more infohttp://www.plantcell.org/content/10/5/825
Manipulation of the napin primary structure alters its packaging and deposition in transgenic tobacco (Nicotiana tabacum L.)...  Manipulation of the napin primary structure alters its packaging and deposition in transgenic tobacco (Nicotiana tabacum L.)...
Plant Molecular Biology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic ... Modified 2S albumins with improved tryptophan content are correctly expressed in transgenic tobacco plants ... Expression and processing of an Arabidopsis 2S albumin in transgenic tobacco. De Clercq, A.; Vandewiele, M.; De Rycke, R.; Van ... Enkephalins produced in transgenic plants using modified 2S seed storage proteins. Vandekerckhove, J.; Van Damme, J.; Van ...
more infohttps://www.deepdyve.com/lp/springer_journal/manipulation-of-the-napin-primary-structure-alters-its-packaging-and-WYmJGPqVR2
STUDIES OF THE ROLE OF THE PROPEPTIDES OF THE ARABIDOPSIS-THALIANA 2S ALBUMIN.  STUDIES OF THE ROLE OF THE PROPEPTIDES OF THE ARABIDOPSIS-THALIANA 2S ALBUMIN.
1993). STUDIES OF THE ROLE OF THE PROPEPTIDES OF THE ARABIDOPSIS-THALIANA 2S ALBUMIN. PLANT PHYSIOLOGY, 102(2), 425-433. ... "STUDIES OF THE ROLE OF THE PROPEPTIDES OF THE ARABIDOPSIS-THALIANA 2S ALBUMIN." PLANT PHYSIOLOGY 102.2 (1993): 425-433. Print. ... "STUDIES OF THE ROLE OF THE PROPEPTIDES OF THE ARABIDOPSIS-THALIANA 2S ALBUMIN." Plant Physiology 102 (2): 425-433. ... STUDIES OF THE ROLE OF THE PROPEPTIDES OF THE ARABIDOPSIS-THALIANA 2S ALBUMIN. PLANT PHYSIOLOGY. 1993;102(2):425-33. ...
more infohttps://biblio.ugent.be/publication/202158
Delivery of Prolamins to the Protein Storage Vacuole in Maize Aleurone Cells | Plant Cell  Delivery of Prolamins to the Protein Storage Vacuole in Maize Aleurone Cells | Plant Cell
The 2S albumin large chain antibody (Scarafoni et al., 2001) was used in a 1:300 dilution, and it was kindly donated by A. ... Your Name) has sent you a message from Plant Cell Message Body (Your Name) thought you would like to see the Plant Cell web ... 2005). Autophagic recycling: Lessons from yeast help define the process in plants. Curr. Opin. Plant Biol. 8: 165-173. ... 2004). Processing of ATG8s, ubiquitin-like proteins, and their deconjugation by ATG4s are essential for plant autophagy. Plant ...
more infohttp://www.plantcell.org/content/23/2/769.long
Protein sorting and expression of a unique soybean cotyledon protein, GmSBP, destined for the protein storage vacuole, Plant...  Protein sorting and expression of a unique soybean cotyledon protein, GmSBP, destined for the protein storage vacuole, Plant...
Plant Molecular Biology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic ... Vesicle transport and processing of the precursor to 2S albumin in pumpkin ... Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology. ISSN. 0167-4412. eISSN. 1573-5028. D.O.I.. 10.1023/A: ... Program in Plant Physiology, School of Molecular Sciences, Washington State University, Pullman, WA 99164- ...
more infohttps://www.deepdyve.com/lp/springer_journal/protein-sorting-and-expression-of-a-unique-soybean-cotyledon-protein-aVOAWoivPC
Clustering of Microarray Data Reveals Transcript Patterns Associated with Somatic Embryogenesis in Soybean | Plant Physiology  Clustering of Microarray Data Reveals Transcript Patterns Associated with Somatic Embryogenesis in Soybean | Plant Physiology
Genes for seed proteins such as glycinins, lipoxygenases, Kunitz trypsin inhibitors, and 2S albumin were also up-regulated in ... 1997) Biosynthesis and action of jasmonates in plants. Annu Rev Plant Physiol Plant Mol Biol 48:355-381. ... 2000) Plant glutathione S-transferases: enzymes with multiple functions in sickness and in health. Trends Plant Sci 5:193-198. ... Set 4 includes conglycinin genes, a Met-rich 2S albumin, but also lipoxygenase 1, 2, and 3. Lipoxygenases constitute 1% to 2% ...
more infohttp://www.plantphysiol.org/content/132/1/118
In Defense against Pathogens. Both Plant Sentinels and Foot Soldiers Need to Know the Enemy | Plant Physiology  In Defense against Pathogens. Both Plant Sentinels and Foot Soldiers Need to Know the Enemy | Plant Physiology
Plant nonspecific lipid transfer proteins, 2S albumins, proteinase inhibitors, and puroindolines act synergistically with ... Both Plant Sentinels and Foot Soldiers Need to Know the Enemy Message Subject (Your Name) has sent you a message from Plant ... 1994) The physiology and molecular biology of plant 1,3-β-d-glucanases and 1,3;1,4-β-d-glucanases. Crit Rev Plant Sci 13:325- ... 1999) Plants have a sensitive perception system for the most conserved domain of bacterial flagellin. Plant J 18:265-276. ...
more infohttp://www.plantphysiol.org/content/131/4/1580
A novel chitin-binding protein from Moringa oleifera seed with potential for plant disease control. - Semantic Scholar  A novel chitin-binding protein from Moringa oleifera seed with potential for plant disease control. - Semantic Scholar
Altogether, these results suggest that Mo-CBP3 might be involved in plant defense mechanisms and could be used as potential ... antifungal agent for controlling fungal pathogens in plants. ... Is a Member of the 2S Albumin Family. *José Ednésio da Cruz ... Plant antifungal proteins and their applications in agriculture. *Juan Yan, Su-su Yuan, Luan-luan Jiang, Xiu-juan Ye, T. B. Ng ... A novel chitin-binding protein from Moringa oleifera seed with potential for plant disease control.. @article{Gifoni2012ANC, ...
more infohttps://www.semanticscholar.org/paper/A-novel-chitin-binding-protein-from-Moringa-seed-Gifoni-Oliveira/c562451883166543d5ea0d911b36438728cc0f1f
Modified Bean Seed Protein Phaseolin Did Not Accumulate Stably in Transgenic  Tobacco Seeds after Methionine  Enhancement...  Modified Bean Seed Protein Phaseolin Did Not Accumulate Stably in Transgenic Tobacco Seeds after Methionine Enhancement...
... regenerating transgenic tobacco plants, and examining the accumulation of phaseolin protein in mature transgenic tobacco seeds ... Modified 2S Albumins with Improved Tryptophan Content Are Correctly Expressed in Transgenic Tobacco Plants. Federation of ... Endoplasmic Reticulum Protein Quality and Its Relationship to Environmental Stress Responses in Plants. Plant Cell, 22, 2930- ... 3Plant Physiology and Genetic Research Unit, US Arid-Land Agricultural Research Center, USDA-ARS, Maricopa, AZ, USA.. ...
more infohttps://www.scirp.org/Journal/PaperInformation.aspx?PaperID=54719
  • 9) Similarly to other 2S albumins, cashew allergens strongly resist heat denaturation (roasting) and hydrolysis by digestive proteases (pepsin, trypsin), and thus display a high allergenic potency. (phadia.com)
  • Do Nascimento V.V., Castro H.C., Abreu P.A., Oliveira A.E., Fernandez J.H., Araujo Jda S. & Machado O.L. 2011: In silico structural characteristics and α-amylase inhibitory properties of Ric c 1 and Ric c 3, allergenic 2S albumins from Ricinus communis seeds. (eje.cz)
  • It was suggested that "germin" , {first found and only known to occur in the "true cereals": barley, corn, oat, rice, and wheat} a plant enzyme, oxalate oxidase 'one-very-tough-little- protein' as such an ancestor. (wikipedia.org)
  • Besides its biological relevance as a model to study plant development, cell differentiation, and programmed cell death, the cereal endosperm is very important in terms of its nutritional value. (plantcell.org)
  • Franco O.L., Rigden D.J., Melo F.R. & Grossi-de-Sa M.F. 2002: Plant α-amylase inhibitors and their interaction with insect α-amylase. (eje.cz)
  • Frequently, not only single miRNAs but whole families of closely related miRNAs have been found in animals and plants. (biomedcentral.com)
  • The largest differences in nutrient composition were found between the two conventional breeds and between conventional Desiree and its near-isogenic genetically modified potato plant, indicating that genetic modification as well as conventional breeding can influence nutrient composition. (scirp.org)
  • Foliar diseases of sesame such as Alternaria leaf blight may cause significant yield losses, with reductions in plant health and seed quality. (ppjonline.org)
  • The pathogenicity and virulence of these isolates of Alternaria alternata was confirmed in inoculations of sesame plants resulting in typical symptoms of leaf blight disease. (ppjonline.org)
  • 8. The method of any one of claim 1 or 2, wherein the plant characterized by sialic acid-mediated systemic acquired resistance (SA-mediated SAR), comprises a monocot or dicot. (patentsencyclopedia.com)
  • The chimeric genes have been expressed in tobacco plants under the control of the seed-specific napA gene promoter. (deepdyve.com)
  • In these specific host-pathogen interactions, resistance to a particular pathogen is conditional on the presence of a specific Avr (avirulence) gene of the pathogen and a specific R (resistance) gene (usually a single dominant gene) in the plant host. (plantphysiol.org)
  • In recent years, predominantly transgenic plants of the so-called first generation, with input traits like insect resistance or herbicide tolerance, were generated, cultivated and analyzed . (scirp.org)
  • It is still uncertain whether plant surveillance systems utilize molecular pattern recognition to identify pathogen challenges. (plantphysiol.org)
  • We now understand that the Avr gene system of potential plant pathogens directly or indirectly provides a biochemical target for a plant surveillance system in which the R gene plays a central role. (plantphysiol.org)