2S Albumins, Plant: A major class of water-soluble seed storage proteins. Many proteins from this class are major PLANT ALLERGENS.Albumins: Water-soluble proteins found in egg whites, blood, lymph, and other tissues and fluids. They coagulate upon heating.Antigens, Plant: Substances found in PLANTS that have antigenic activity.Plants, Genetically Modified: PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.Serum Albumin: A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.Sesamum: A plant genus of the family PEDALIACEAE that is the source of the edible seed and SESAME OIL.Plant Proteins: Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.Bertholletia: A plant genus of the family Lecythidaceae which is the source of edible Brazil nuts.Nuts: Botanically, a type of single-seeded fruit in which the pericarp enclosing the seed is a hard woody shell. In common usage the term is used loosely for any hard, oil-rich kernel. Of those commonly eaten, only hazel, filbert, and chestnut are strictly nuts. Walnuts, pecans, almonds, and coconuts are really drupes. Brazil nuts, pistachios, macadamias, and cashews are really seeds with a hard shell derived from the testa rather than the pericarp.Plant Leaves: Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)Seeds: The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield.Plants, Toxic: Plants or plant parts which are harmful to man or other animals.Plant Roots: The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)Genes, Plant: The functional hereditary units of PLANTS.Serum Albumin, Bovine: Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)Arachis hypogaea: A plant species of the family FABACEAE that yields edible seeds, the familiar peanuts, which contain protein, oil and lectins.Plant Extracts: Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard.Plant Shoots: New immature growth of a plant including stem, leaves, tips of branches, and SEEDLINGS.Protein PrecursorsPlants, Medicinal: Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals.DNA, Plant: Deoxyribonucleic acid that makes up the genetic material of plants.Plant Development: Processes orchestrated or driven by a plethora of genes, plant hormones, and inherent biological timing mechanisms facilitated by secondary molecules, which result in the systematic transformation of plants and plant parts, from one stage of maturity to another.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Plant Cells: Basic functional unit of plants.Plant Stems: Parts of plants that usually grow vertically upwards towards the light and support the leaves, buds, and reproductive structures. (From Concise Dictionary of Biology, 1990)Genome, Plant: The genetic complement of a plant (PLANTS) as represented in its DNA.Arabidopsis: A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Plants, Edible: An organism of the vegetable kingdom suitable by nature for use as a food, especially by human beings. Not all parts of any given plant are edible but all parts of edible plants have been known to figure as raw or cooked food: leaves, roots, tubers, stems, seeds, buds, fruits, and flowers. The most commonly edible parts of plants are FRUIT, usually sweet, fleshy, and succulent. Most edible plants are commonly cultivated for their nutritional value and are referred to as VEGETABLES.Plant Structures: The parts of plants, including SEEDS.Plant Growth Regulators: Any of the hormones produced naturally in plants and active in controlling growth and other functions. There are three primary classes: auxins, cytokinins, and gibberellins.Deuterium: Deuterium. The stable isotope of hydrogen. It has one neutron and one proton in the nucleus.Magnetic Resonance Spectroscopy: Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).Environmental Health: The science of controlling or modifying those conditions, influences, or forces surrounding man which relate to promoting, establishing, and maintaining health.Climate Change: Any significant change in measures of climate (such as temperature, precipitation, or wind) lasting for an extended period (decades or longer). It may result from natural factors such as changes in the sun's intensity, natural processes within the climate system such as changes in ocean circulation, or human activities.Deuterium Oxide: The isotopic compound of hydrogen of mass 2 (deuterium) with oxygen. (From Grant & Hackh's Chemical Dictionary, 5th ed) It is used to study mechanisms and rates of chemical or nuclear reactions, as well as biological processes.Climate: The longterm manifestations of WEATHER. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Isotopes: Atomic species differing in mass number but having the same atomic number. (Grant & Hackh's Chemical Dictionary, 5th ed)Food Hypersensitivity: Gastrointestinal disturbances, skin eruptions, or shock due to allergic reactions to allergens in food.Organizations, Nonprofit: Organizations which are not operated for a profit and may be supported by endowments or private contributions.Food: Any substances taken in by the body that provide nourishment.Voluntary Health Agencies: Non-profit organizations concerned with various aspects of health, e.g., education, promotion, treatment, services, etc.Eating Disorders: A group of disorders characterized by physiological and psychological disturbances in appetite or food intake.Food Habits: Acquired or learned food preferences.Foundations: Organizations established by endowments with provision for future maintenance.Tobacco: A plant genus of the family SOLANACEAE. Members contain NICOTINE and other biologically active chemicals; its dried leaves are used for SMOKING.Tobacco Mosaic Virus: The type species of TOBAMOVIRUS which causes mosaic disease of tobacco. Transmission occurs by mechanical inoculation.Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.Enzymes: Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however CATALYTIC RNA and CATALYTIC DNA molecules have also been identified.Spheroplasts: Cells, usually bacteria or yeast, which have partially lost their cell wall, lost their characteristic shape and become round.Saccharomyces cerevisiae Proteins: Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.Cell Wall: The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.

Co-introduction of an antisense gene for an endogenous seed storage protein can increase expression of a transgene in Arabidopsis thaliana seeds. (1/59)

We have investigated whether the expression in Arabidopsis thaliana seeds of a transgene (the Phaseolus vulgaris arcelin (arc)5-I gene) could be enhanced by the simultaneous introduction of an antisense gene for an endogenous seed storage protein (2S albumin). Seeds of plants transformed with both the arc5-I gene and a 2S albumin antisense gene contained reduced amounts of 2S albumins and increased arcelin-5 (Arc5) accumulation levels compared to lines harboring the arc5-I gene only. Arc5 production could be enhanced to more than 24% of the total seed protein content, suggesting that antisense technology could be of great utility to favor high expression of transgenes.  (+info)

Direct kinetic evidence for folding via a highly compact, misfolded state. (2/59)

The 2 S seed storage protein, sunflower albumin 8 (SFA-8), contains an unusually high proportion of hydrophobic residues including 16 methionines (some of which may form a surface hydrophobic patch) in a disulfide cross-linked, alpha-helical structure. Circular dichroism and fluorescence spectroscopy show that SFA-8 is highly stable to denaturation by heating or chaotropic agents, the latter resulting in a reversible two-state unfolding transition. The small m(U) (-4.7 M(-1) at 10 degrees C) and DeltaC(p) (-0.95 kcal mol(-1) K(-1)) values indicate that relatively little nonpolar surface of the protein is exposed during unfolding. Commensurate with the unusual distribution of hydrophobic residues, stopped-flow fluorescence data show that the folding pathway of SFA-8 is highly atypical, in that the initial product of the rapid collapse phase of folding is a compact nonnative state (or collection of nonnative states) that must unfold before acquiring the native conformation. The inhibited folding reaction of SFA-8, in which the misfolded state (m(M) = -0.95 M(-1) at 10 degrees C) is more compact than the transition state for folding (m(T) = -2.5 M(-1) at 10 degrees C), provides direct kinetic evidence for the transient misfolding of a protein.  (+info)

GerN, an antiporter homologue important in germination of Bacillus cereus endospores. (3/59)

A homologue of the grmA spore germination gene of Bacillus megaterium and of a NaH-antiporter gene (napA) of Enterococcus hirae has been identified in Bacillus cereus 569 (ATCC 10876). The putative protein product has 58 and 43% amino acid identity with GrmA and NapA, respectively. Insertional inactivation of this B. cereus gene, named gerN, did not affect vegetative growth or sporulation. The null mutant spores were 30-fold slower to germinate in inosine (5 mM) but germinated almost normally in response to L-alanine (10 mM). The null mutant spores germinated after several hours with inosine as the sole germinant, but germination was asynchronous and the normal order of germination events was perturbed. At a suboptimal germinant concentration (50 microM), inosine germination was completely blocked in the mutant, while the rate of germination in 50 microM L-alanine was reduced to one-third of that of the wild type. The requirement for GerN function in the response to a particular germinant suggests that a germination receptor may have a specifically associated antiporter, which is required at the initiation of germination and which, in the case of the inosine receptor, is GerN. Since germination in suboptimal concentrations of L-alanine shows a delay, additional germination transporters may be required for optimal response at low germinant concentrations.  (+info)

Calcium-mediated association of a putative vacuolar sorting receptor PV72 with a propeptide of 2S albumin. (4/59)

PV72, a type I membrane protein with three epidermal-growth factor (EGF)-like motifs, was found to be localized on the membranes of the precursor-accumulating (PAC) vesicles that accumulated precursors of various seed storage proteins. To clarify the function of PV72 as a sorting receptor, we expressed four modified PV72s and analyzed their ability to bind the internal propeptide (the 2S-I peptide) of pro2S albumin by affinity chromatography and surface plasmon resonance. The recombinant PV72 specifically bound to the 2S-I peptide with a K(D) value of 0.2 microm, which was low enough for it to function as a receptor. The EGF-like motifs modulated the Ca(2+)-dependent conformational change of PV72 to form a functional pocket for the ligand binding. The binding of Ca(2+) stabilizes the receptor-ligand complex even at pH 4.0. The association and dissociation of PV72 with the ligand is modulated by the Ca(2+) concentration (EC(50) value = 40 microm) rather than the environmental pH. Overall results suggest that Ca(2+) regulates the vacuolar sorting mechanism in higher plants.  (+info)

Recombinant pronapin precursor produced in Pichia pastoris displays structural and immunologic equivalent properties to its mature product isolated from rapeseed. (5/59)

2S albumin storage proteins from rapeseed (Brassica napus), called napins, consist of two different polypeptide chains linked by disulphide bridges, which are derived by proteolytic cleavage from a single precursor. The precursor form of the napin BnIb (proBnIb) has been cloned using a PCR strategy and sequenced. The amino-acid sequence deduced from the clone includes 31 residues of the small chain and 75 of the large chain, which are connected by the peptide Ser-Glu-Asn. Expression of the cDNA encoding proBnIb has been carried out in the methylotrophic yeast Pichia pastoris. The induced protein was secreted to the extracellular medium at a yield of 80 mg.L(-1) of culture and was purified by means of size-exclusion chromatography and reverse phase-HPLC. Recombinant proBnIb appeared properly folded as its molecular and spectroscopic properties were equivalent to those of the mature heterodimeric protein. As 2S albumin storage proteins from Brassicaceae have been shown to be type I allergy inducers, the immunological activity of the recombinant proBnIb was analysed as a measure of its structural integrity. The immunological properties of the recombinant precursor and the natural napin were indistinguishable by immunoblotting and ELISA inhibition using polyclonal antisera and sera of patients allergic to mustard and rapeseed. In conclusion, the recombinant expression of napin precursors in P. pastoris has been shown to be a successful method for high yield production of homogeneous and properly folded proteins whose polymorphism and complex maturation process limited hitherto their availability.  (+info)

Protein structure plays a critical role in peanut allergen stability and may determine immunodominant IgE-binding epitopes. (6/59)

Hypersensitivity to peanuts is a reaction mediated by IgE Abs in response to several peanut protein allergens. Among these allergenic proteins, Ara h 2 is one of the most commonly recognized allergens. Ara h 2 is a 17-kDa protein that has eight cysteine residues that could form up to four disulfide bonds. Circular dichroism studies showed substantial changes in the secondary and tertiary structures of the reduced Ara h 2 as compared with the native protein. Upon treatment with trypsin, chymotrypsin, or pepsin, a number of relatively large fragments are produced that are resistant to further enzymatic digestion. These resistant Ara h 2 peptide fragments contain intact IgE-binding epitopes and several potential enzyme cut sites that are protected from the enzymes by the compact structure of the protein. The enzyme-treated allergen remains essentially intact despite the action of proteases until the fragments are dissociated when the disulfide linkages are reduced. Amino acid sequence analysis of the resistant protein fragments indicates that they contain most of the immunodominant IgE-binding epitopes. These results provide a link between allergen structure and the immunodominant IgE-binding epitopes within a population of food-allergic individuals.  (+info)

Engineered recombinant peanut protein and heat-killed Listeria monocytogenes coadministration protects against peanut-induced anaphylaxis in a murine model. (7/59)

Peanut allergy (PNA) is the major cause of fatal and near-fatal anaphylactic reactions to foods. Traditional immunotherapy using peanut (PN) protein is not an option for PNA therapy because of the high incidence of adverse reactions. We investigated the effects of s.c. injections of engineered (modified) recombinant PN proteins and heat-killed Listeria monocytogenes (HKLM) as an adjuvant on anaphylactic reactions in a mouse model of PN allergy. PN-allergic C3H/HeJ mice were treated s.c. with a mixture of the three major PN allergens and HKLM (modified (m)Ara h 1-3 plus HKLM). The effects on anaphylactic reactions following PN challenge and the association with Ab levels and cytokine profiles were determined. Although all mice in the sham-treated groups exhibited anaphylactic symptoms with a median symptom score of 3, only 31% of mice in the mAra h 1-3 plus HKLM group developed mild anaphylaxis, with a low median symptom score of 0.5. Alterations in core body temperature, bronchial constriction, plasma histamine, and PN-specific IgE levels were all significantly reduced. This protective effect was markedly more potent than in the mAra h 1-3 protein alone-treated group. HKLM alone did not have any protective effect. Reduced IL-5 and IL-13, and increased IFN-gamma levels were observed only in splenocytes cultures from mAra h 1-3 plus HKLM-treated mice. These results show that immunotherapy with modified PN proteins and HKLM is effective for treating PN allergy in this model, and may be a potential approach for treating PNA.  (+info)

A plant-based allergy vaccine suppresses experimental asthma via an IFN-gamma and CD4+CD45RBlow T cell-dependent mechanism. (8/59)

Allergic asthma is currently considered a chronic airway inflammatory disorder associated with the presence of activated CD4(+) Th2-type lymphocytes, eosinophils, and mast cells. Interestingly, therapeutic strategies based on immune deviation and suppression have been shown to successfully attenuate the development of the asthma phenotype. In this investigation, we have for the first time used a genetically modified (GM) plant, narrow leaf lupin (Lupinus angustifolius L.), expressing a gene for a potential allergen (sunflower seed albumin) (SSA-lupin) to examine whether a GM plant/food-based vaccine strategy can be used to suppress the development of experimental asthma. We show that oral consumption of SSA-lupin promoted the induction of an Ag-specific IgG2a Ab response. Furthermore, we demonstrate that the plant-based vaccine attenuated the induction of delayed-type hypersensitivity responses and pathological features of experimental asthma (mucus hypersecretion, eosinophilic inflammation, and enhanced bronchial reactivity (airways hyperreactivity). The suppression of experimental asthma by SSA-lupin was associated with the production of CD4(+) T cell-derived IFN-gamma and IL-10. Furthermore, we show that the specific inhibition of experimental asthma was mediated via CD4(+)CD45RB(low) regulatory T cells and IFN-gamma. Thus, our data demonstrate that a GM plant-based vaccine can promote a protective immune response and attenuate experimental asthma, suggesting that plant-based vaccines may be potentially therapeutic for the protection against allergic diseases.  (+info)

Presented in this dissertation are comprehensive studies of the structures of the peanut allergen protein Ara h 2 and the effect of food processing (roasting) on it. A detailed elucidation of the primary structure and PTM of Ara h 2 from the raw peanuts has been described. Ara h 2 isoforms were purified and cleaved via microwave accelerated trypsin digestion. The peptide mixtures were analyzed by LC-MS/MS and targeted CID. De novo sequencing of the MS/MS spectra revealed the protein sequence of each Ara h 2 isoform. Several hydroxyproline sites have been discovered while disulfide bond structures have been partially determined. Using anti-Ara h 2 antibodies, Western blotting of 1-D gels of the raw and dark roasted peanuts was carried out in order to characterize the changes of Ara h 2 between these two samples. The result indicates that Ara h 2 may present in a much heavier form in the roasted peanuts, possibly due to crosslinking and aggregation with other proteins. Subsequent LC-MS/MS studies ...
A competitive lateral flow assay for detecting the major peanut allergen, Ara h1, has been developed. The detector reagents are Ara h1-tagged liposomes, and the capture reagents are anti-Ara h1 polyclonal antibodies. Two types of rabbit polyclonal antibodies were raised either against the entire Ara h1 molecules (anti-Ara h1 Ab) or against an immunodominant epitope on Ara h1 (anti-peptide Ab). All of them reacted specifically with Ara h1 in Western Blot against crude peanut proteins. Moreover, the anti-Ara h1 Ab was chosen for this assay development because of its highest immunoactivity to Ara h1-tagged liposomes in the lateral flow assay. The calculated limit of detection (LOD) of this assay is 0.45 μg mLâˆ1 of Ara h1 with a dynamic range between 0.1 and 10 μg mLâˆ1 of Ara h1 in buffer. Additionally, the visually determined detection range is from 1 to 10 μg mLâˆ1 of Ara h1 in buffer. Results using this assay can be obtained within 30 min without the need of sophisticated ...
Despite the skeleton of cysteine residues of 2S albumins being highly conserved, a relatively low amino acid sequence homology, within and among plant species, has been demonstrated. (9) Overall, the degree of sequence homology of 2S allergens ranges from 14 to 40%, and does not reflect phylogenetic relationships, with the exception of 2S albumins belonging to the Brassicaceae family (i.e. Bra j 1, Bra n 1 and Sin a 1). (9). Although 2S albumins have high structural homology, cross-reactivity seems to be uncommon in this protein family. (9). For example, Ber e 1, a 2S albumin, a major allergen of Brazil nut, has been shown to have the following homology with 2S albumins from other tree and ground nuts: Ara h 2 (40%), Ara h 6 (48%), Ana o 3 (61%), Car i 1 (66%) and Jug r 1 (68%). (14) Yet in a study evaluating the ability of Brazil nut to react against other nuts (almond, hazelnut, pecan, cashew, walnut and peanut) or legumes (pea and chickpea), the cross-reactivity of antisera against all ...
Ara h 1, a major peanut allergen, is known as a stable trimeric protein. Nevertheless, upon purification of native Ara h 1 from peanuts using only size exclusion chromatography, the allergen appeared to exist in an oligomeric structure, rather than as a trimeric structure. The oligomeric structure was independent of the salt concentration applied. Subjecting the allergen to anion exchange chromatography induced the allergen to dissociate into trimers. Ammonium sulfate precipitation did not bring about any structural changes, whereas exposing the allergen to hydrophobic interaction chromatography caused it to partly dissociate into trimers, with increasing amounts of trimers at higher ionic strengths. The (partial) dissociation into trimers led to a change in the tertiary structure of the monomeric subunits of the allergen, with the monomers in Ara h 1 oligomers having a more compact tertiary structure compared with the monomers in Ara h 1 trimers. As structural characteristics are important for ...
Ara h3 belongs to the glycinin family of seed storage proteins and is one of the major peanut allergens. It comprises over 20% of the total peanut protein mass, making it a logical target for the detection of trace quantities of undeclared peanut contamination in foods. Both Ara h1 and Ara h3 are detected in lower quantities in cooked foods, either because of the failure to completely resolubilize the denatured proteins or because of the disruption of conformational epitopes required for monoclonal antibody recognition. A new reagent containing a proprietary non-detergent sulfobetaine (NDSB) is described which solubilizes more total protein and yields more Ara h3 protein from both raw and roasted peanut than other commonly used ELISA-compatible reagents.
Recombinant Allergen Ara h 1.0101 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 63,484 Dalton.
Peanut allergy is a significant health problem because of the prevalence and severity of the reaction. The only therapeutic option currently available for the prevention of a peanut hypersensitivity reaction is food avoidance. Therefore, the characterization of peanut allergens is important for developing diagnostic and therapeutic capabilities. As a basis for the biochemical and biophysical studies of the two allergenic proteins, a recombinant expression strategy of authentically folded Ara h 2 and Ara h 6 was established in this work. This efficient large-scale method exploiting on special E. coli strains with an oxidizing cytoplasm is clearly suited for recombinant expression of properly folded and disulfide bridged peanut 2S albumins and highly likely other members of this protein family. The intact secondary and tertiary structure, correct disulfide pattern and immunological reactivity of the recombinant proteins was confirmed using the native Ara h 2 protein from peanut as a reference. The ...
Heating Ara h 2/6 at 110°C resulted in extensive denaturation whilst Ara h 2/6 extracted from roasted peanut retained its native conformation. Allergen stimulation of PBMC from peanut allergic patients induced proliferation of mainly CD4+ T-cells and induction of Th2 cytokine secretion which was unaffected by thermal processing. IgE reactivity and functionality of Ara h 2/6 was decreased by heating. Whilst heating-glycation further reduced the IgE binding capacity of the proteins, it moderated their loss of histamine releasing capacity. Ara h 2/6 purified from roasted peanut demonstrated the same IgE reactivity as unheated, native Ara h 2/6. ...
Prefers hydrophobic residues Phe, Val, Ile, Leu, and Ala at P1 and P1, but also cleaves -Phe-,-Asp- and -Asp-,-Asp- bonds in 2S albumin from plant seeds ...
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Learn Jr. Sx- Anesthesia Know Hows 2 facts using a simple interactive process (flashcard, matching, or multiple choice). Finally a format that helps you memorize and understand. Browse or search in thousands of pages or create your own page using a simple wizard. No signup required!
SX-200 series is a silicone and olefin thermoplastic moulding material for injection moulding of automotive interior and exterior parts like seals and grips and construction sash window seals.. Features:. ...
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BACKGROUND: The 2S albumin Ber e 1 is the major allergen in Brazil nuts. Previous findings indicated that the protein alone does not cause an allergenic response in mice, but the addition of components from a Brazil nut lipid fraction were required. Structural details of Ber e 1 may contribute to the understanding of the allergenic properties of the protein and its potential interaction partners. METHODOLOGY/PRINCIPAL FINDINGS: The solution structure of recombinant Ber e 1 was solved using NMR spectroscopy and measurements of the protein back bone dynamics at a residue-specific level were extracted using (15)N-spin relaxation. A hydrophobic cavity was identified in the structure of Ber e 1. Using the paramagnetic relaxation enhancement property of Cu(2+) in conjunction with NMR, it was shown that Ber e 1 is able to specifically interact with the divalent copper ion and the binding site was modeled into the structure. The IgE binding region as well as the copper binding site show increased ...
Allergic reactions to food are on the rise worldwide and there is a corresponding increase in interest to understand the molecular mechanisms responsible. Peanut allergies are the most problematic because the reaction often persists into adulthood and can be as severe as anaphylaxis and death. The purpose of the work presented here was to develop a reproducible method to produce large quantities of pure recombinant Ara h 1(rAra h 1) that will enable standardization of immunological tests for patients and allow structural and immunological studies on the wild type and mutagenized forms of the protein. Ara h 1 is initially a pre-pro-protein which, following two endoproteolytic cleavages, becomes the mature form found in peanut. The mature form however has flexible regions that make it refractory to some structural studies including crystallography. Therefore, independent purification of the mature and core regions was desirable. Expression constructs were synthesized cDNA clones for each in a pET plasmid
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RICHMOND, Va. -- Rold Gold pretzel products sold nationwide have been recalled over concerns that an ingredient not listed could be dangerous to people with allergies.
Protein solubility was modified by processing and extraction was optimal using harsh denaturing conditions. The 2D protein profiles were reflective of processing type and comparison to raw peanut highlighted spot to spot variations. Roasting caused aggregation leading to smearing but most notable was the apparent loss of proteins including Ara h 1 and Ara h 2/6. Boiling resulted in a loss of proteins into the cooking water and was most extensive in samples boiled for 4-6 hours. Serum IgE from peanut allergic patients bound to Ara h 1, Ara h 3 and aggregates in both roasted and extensively boiled peanuts. In contrast IgE reactivity to Ara h 2/6 in the seeds was lost in boiled peanuts but retained to protein in cooking water. ...
... The amount of protein in pecan nut is about 9%. Some of these proteins are known for being allergenic, like the 2S albumin Car I 1 or the 11S legumin Car I 4 . Compared to other nuts the allergenic potential of pecan nut
Still I Rise on postuumisti julkaistu yhteistyöalbumi 2Pacin ja hänen ryhmänsä Outlawzien kanssa, lukuun ottamatta alkuperäisjäsentä Hussein Fatalia, joka jätettiin pois levyltä tuntemattomista syistä. Albumin kaikki kappaleet ovat ennen julkaisemattomia. Still I Risen julkaisi 14. joulukuuta 1999 Interscope Records, Death Row Recordsin alaisuudessa, ja se saavutti platinarajan myytyään 1,5 miljoonaa kappaletta. Albumilta julkaistiin single "Baby Dont Cry (Keep Ya Head Up II)", joka on samantyyppinen tunnelmasoinnultaan kuin 2Pacin suositut singlet "Brendas Got a Baby" ja "Keep Ya Head Up". "Baby Dont Cry (Keep Ya Head Up II)" on myös albumin ainoa kappale, josta kuvattiin musiikkivideo. ...
Forhøjet ammonium er resultatet af en defekt urinstofcyklus, som kan være forårsaget af erhvervede eller arvelige sygdomme. En erhvervet defekt opstår sekundært til leversygdom, mens de arvelige årsager inkluderer defekter i et af de seks enzymer i urinstofcyklus samt sygdomme, herunder organiske acidæmier, ved hvilke der enten dannes metabolitter, der inhiberer cyklus eller forårsager en mangel på metabolitter afgørende for urinstofcyklus funktion. De hyppigste af urinstofcyklusenzymdefekterne er ornithintranskarbamylasedefekt, carbamylfosfatsyntetasedefekt samt argininosuccinatsyntasedefekt. For alle disse defekter forekommer neonatal debut ofte, men debut kan ses i alle aldre. De kliniske symptomer er primært forårsaget af hyperammoniæmi med encefalopati, koma og cerebralt ødem i de svære tilfælde, mens der ved de senere debuterende ofte mildere tilfælde ses intermitterende neurologiske og psykiatriske problemer samt gastro-intestinale og hepatiske symptomer, og på længere ...
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The IUPHAR/BPS Guide to Pharmacology. SX-517 ligand page. Quantitative data and detailed annnotation of the targets of licensed and experimental drugs.
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Das eg-Allel codiert eine Fellzeichnung, die beim Afghanen Domino und beim Saluki Grizzle genannt wird. Es wurde bislang ausschließlich in diesen beiden Rassen und beim Barsoi nachgewiesen. Das eg-Allel ist rezessiv gegenüber EM und E, aber dominant gegenüber e. Die typische Domino-Zeichnung ...
Tuğba Eray Biber, Karadeniz Rumları ve Yunanistan, İstanbul: Yeditepe Yayınları, 2016, XXXIV+315 s., ISBN: 978-605-9787-49-9 ...
Resistance to proteolytic enzymes and heat is thought to be a prerequisite property of food allergens. Allergens from peanut (Arachis hypogaea) are the most frequent cause of fatal food allergic reactions. The allergenic 2S albumin Ara h 2 and the homologous minor allergen Ara h 6 were studied at the molecular level with regard to allergenic potency of native and protease-treated allergen. A high-resolution solution structure of the protease-resistant core of Ara h 6 was determined by NMR spectroscopy, and homology modelling was applied to generate an Ara h 2 structure. Ara h 2 appeared to be the more potent allergen, even though the two peanut allergens share substantial cross-reactivity. Both allergens contain cores that are highly resistant to proteolytic digestion and to temperatures of up to 100 °C. Even though IgE antibody-binding capacity was reduced by protease treatment, the mediator release from a functional equivalent of a mast cell or basophil, the humanized RBL (rat basophilic ...
Ara h 1 is a seed storage protein from Arachis hypogaea (peanuts). It is a heat stable 7S vicilin-like globulin with a stable trimeric form that comprises 12-16% of the total protein in peanut extracts. Ara h 1 is known because sensitization to it was found in 95% of peanut-allergic patients from North America. In spite of this high percentage, peanut-allergic patients of European populations have fewer sensitizations to Ara h 1. Ara h 1 is a vicilin, located in the protein fraction of the peanut cotyledon. Ara h 1 forms homotrimers, and due to its highly stable structure, mediated through hydrophobic interactions, it has been established as an allergen. Hydrophobic residues on α-helical bundles are located on the ends of each trimer monomer. Ara h 1 presents an overall pleat like bicupins, N- and C-terminal domains are superposed in 1.9 Å. The molecule has two modules related by an axis perpendicular to the three pleat axes of the trimer. They interact through their hydrophobic parts. The ...
Peanut allergy is a common ailment in the United States. Research suggests that the prevalence of peanut allergy in the United States has doubled over the last 5 years. Currently, the only effective treatment for peanut allergy is a peanut-free diet and quick access to self-injectable epinephrine. This study will evaluate the safety of a rectally administered product, EMP-123, consisting of three recombinant modified peanut protein antigens encapsulated within dead E. coli. E. coli is a common bacterium found in everyones colon. E. coli acts like a package to hold the modified peanut proteins. EMP-123 is designed to act as an allergy vaccine with an eventual goal to induce tolerance to the major peanut proteins responsible for peanut allergy.. This study will involve weekly dosing and for females, a pregnancy test will occur at 48 hours before the start of product administration and later during the study. The study will involve two steps. Step 1 will enroll 5 healthy participants who will ...
The Veratox for Peanut Allergen test is a sandwich enzyme-linked immunosorbent assay (S-ELISA). Peanut protein residue is extracted from samples with a phosphate buffered salt solution (PBS) by shaking in a heated water bath, followed by centrifugation or filtration. Extracted peanut residue is sampled and added to antibody-coated wells (capture antibody) where it binds to the antibody during an incubation. Any unbound peanut residue is washed away and a second antibody (detector antibody), which is enzyme labelled, is added. The detector antibody binds to the already bound peanut residue. After a second wash, substrate is added. Colour develops as a result of the presence of bound detector antibody. Red Stop reagent is added and the colour of the resulting solution is observed. The test is read in a microwell reader to yield optical densities. The optical densities of the controls form a standard curve, and the sample optical densities are plotted against the curve to calculate the exact ...
Peanut allergies diminish with early expose, a new study says. The New England Journal of Medicine announced that when introduced at a young age, children who would likely develop peanut allergies did not contract them. The study was conducted at Kings College in London and involved 600 babies who were prone to allergies.. The babies either already had skin conditions such as eczema or had a family history of peanut allergies. The parents in the study had to feed their infant a peanut protein or avoid it. By five years of age, the children exposed to peanut proteins had significantly lower risk of developing peanut allergies. By eight years of age, most children never developed the allergy. Peanut allergies can be severe, causing anaphylaxis. This involves the inability to breathe because the throat closes up. For this reason, doctors have erred on the side of avoidance, cautioning parents to steer clear of the legume. A less severe reaction to peanut exposure includes hives, digestion problems ...
Obesity is a chronic disease associated to an inflammatory process resulting in oxidative stress that leads to morpho-functional microvascular damage that could be improved by some dietary interventions. In this study, the intake of Brazil nuts (Bertholletia excelsa), composed of bioactive substances like selenium, α- e γ- tocopherol, folate and polyunsaturated fatty acids, have been investigated on antioxidant capacity, lipid and metabolic profiles and nutritive skin microcirculation in obese adolescents. Obese female adolescents (n = 17), 15.4 ± 2.0 years and BMI of 35.6 ± 3.3 kg/m2, were randomized 1:1 in two groups with the diet supplemented either with Brazil nuts [BNG, n = 08, 15-25 g/day (equivalent to 3 to 5 units/day)] or placebo [PG (lactose), n = 09, one capsule/day] and followed for 16 weeks. Anthropometry, metabolic-lipid profiles, oxidative stress and morphological (capillary diameters) and functional [functional capillary density, red blood cell velocity (RBCV) at baseline and peak
Medicinal properties of Brazil nuts Characteristics of brazil nuts Common English name: Brazil-nut Tree, Para Nut, Brazilnuts (fruit), Juvia (tree) Ilustration of Brazil-nut tree - Spanish / Castellano: Nuez de Brasil, nuez amazónica o nuez del Amazonas, castaña de monte, tuercas
Brazil Nut is a nutritional food and must be added to the diet.In this article you can find more health benefits of brazil nuts for skin hair
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the contacts provided below. For general information, Learn About Clinical Studies. ...
was the definitive trial that proved that old approaches to preventing allergies were wrong. The investigators question was formed out of an observation: The risk of peanut allergy among Jewish children in the United Kingdom was 10x as high than Israeli children with similar ancestry. It was noted that while peanuts were generally withheld in the first year of life in the United Kingdom, they tended to be introduced at around 7 months in Israel. The investigators hypothesis was that early introduction of peanut protein into the diet might reduce the risk of peanut allergy.. This was a randomized, open-label study of 640 children in the United Kingdom. To be eligible to participate, children had to be 4 months to 11 months of age, and had severe eczema, egg allergy or both. The average age at screening was 8 months. All children received skin tests for peanut allergy and were then randomly assigned to dietary peanut consumption or avoidance. Children randomized to peanut consumption received 6 ...
Risk of allergy from genetically engineered (GE) foods has been made a major biosafety issue, based on two, but now defunct cases. A gene for the Brazil nut protein was introduced into soybean to increase the content of methionine, an essential amino acid. The serum from people allergic to Brazil nuts cross-reacted with extracts of transgenic soybean and not with extracts of its isogenic. Though no one actually developed allergy by eating the transgenic soybean, since the transgenic is likely to affect people who are allergenic to Brazil nuts, Pioneer Hi-Bred International, the developer of the product, did not proceed with it, an example of self-regulation. The Bt Cry9 protein in the Aventis Starlink Bt corn is more stable in simulated digestion than other Bt proteins, and so it was thought that it might be allergenic. The United States Department of Agriculture (USDA) cleared it for use as both food and feed, but the US Environment Protection Agency (EPA) took a precautionary measure and ...
Dr. Lack: Clearly early consumption of peanuts needs to occur in a safe manner. Peanut was never the first solid introduced into the infants diet but was only introduced once the infant had been accustomed to eating solids over at least a week. Whole peanuts were not given because of the risk of choking. The Israeli snack Bamba (a common puffed corn and peanut food) was commonly used. Peanut butter mixed with fruits in a purée was frequently eaten. Some babies had peanut soup.. Clearly this study has provided great information regarding the development of peanut allergies and the potential for prevention. Questions still remain and future studies will need to be conducted. Dr. Lack was careful to state that the results of the LEAP study answered some questions about those children at high risk for developing peanut allergies. He has an ongoing study called EAT (Enquiring About Tolerance) that will help answer the question about whether or not the results are the same for children without risk ...
Buah Brazil nut yang mencapai usia 14 bulan akan mula gugur... Buahnya bersaiz besar dengan diameter 10-15 cm menyerupai kelapa endocarp dan beratnya mencapai sehingga 2 kilogram... Bijinya dilindungi satu lapisan kulit tebal 8-12 mm yang agak rapuh diikuti pula dengan satu lagi lapisan kulit keras setebal 8-12 mm... Di dalamnya terdapat 8-24 biji yang juga berkulit keras berbentuk seperti ulas limau,,, 4-5 cm panjang... Terdapat 18% protein,,, 13% karbohidrat dan 69% lemak yang terkandung dalam isi Brazil nut... Brazil nut juga digunakan sebagai minyak dalam industri pelincir dan kosmetik...Jumlah selenium yang tinggi di dalam Brazil nut dikatakan baik untuk mencegah kanser prostat,,, kanser payudara dan mencegah risiko terkena sakit jantung... * (selenium bersifat toksik dalam jumlah yang besar). http://en.wikipedia.org/wiki/Brazil_nut. ...
high in protein and essential fatsperhaps the richest dietary source of seleniumexcellent source of dietary fiber and various vitamins; thiamin, Vitamin E, and minerals; magnesium, phosphorus, manganese, zinc High in protein and essential fats, brazil nuts are technically a seed from a fruiting tree. Nutritionally, an
Brazil nuts contain more selenium than any other food. While this sounds like a benefit, you can still have too much of a good thing.
jessica cox | grain free & vegan friendly recipe for strawberry & brazil nut muffins. These muffins are packed with selenium, the healthy thyroid nutrient!
250.00. 50g Inert Blank Matrix certified ,1 ppm total peanut by both Neogen Veratox Peanut and R-Biopharm R6202. Material stores at room temperature and has been additionally evaluated as fit for use with Lateral flow tests.. read more. ...
The report is built using data and information traced from the researchers proprietary databases, company/university websites, clinical trial registries, conferences, SEC filings, investor presentations, and featured press releases from company/university web sites and industry-specific third-party sources, etc.. Peanut Allergy Pipeline. The overall dynamics of the Peanut Allergy market is anticipated to change in the coming years owing to the expected launch of emerging therapies. DBV Technologies, AnaptysBio, Roche, Regeneron Pharmaceuticals, Prota Therapeutics, Camallergy, HAL Allergy B.V., and others are also developing treatments for Peanut allergies. Viaskin Peanut (DBV Technologies) is developed by using proprietary Viaskin Peanut technology platform, which delivers biologically active compounds, including allergens, via intact skin. Therefore, it administers an allergen directly onto the superficial layers of the skin to activate the immune system by specifically targeting ...
Subscription Software as a Service Cloud Portal Platform with two apps for maintaining on-going compliance to the Food Safety Modernization Act (FSMA) & conformity to ISO 22000 with a Single Management System
Some concerns about the use of biotechnology for food production include possible allergic reactions to the transferred protein . For example, if a gene from Brazil nuts that produces an allergen were transferred to soybeans, an individual who is allergic to Brazil nuts might now also be allergic to soybeans. As a result, companies in the United States that develop genetically engineered foods must demonstrate to the U.S. Food and Drug Administration (FDA) that they did not transfer proteins that could result in food allergies . When, in fact, a company attempted to transfer a gene from Brazil nuts to soybeans, the companys tests revealed that they had transferred a gene for an allergen, and work on the project was halted. In 2000 a brand of taco shells was discovered to contain a variety of genetically engineered corn that had been approved by the FDA for use in animal feed, but not for human consumption. Although several antibiotechnology groups used this situation as an example of potential ...
The The Body Shop Brazil Nut soap is mildly scented with a nutty fragrance, of perhaps the Brazil Nut (Not sure of the same as I have no idea of how the Brazil Nut essence smells like), which doesnt linger for long and disappears soon after bath. Though the scent of the soap is pleasant, it is something that I would not prefer, as I personally feel more drawn towards fruity fragrances. It creates abundant creamy lather, helping it to cleanse the skin well. It is easy to rinse off and does so without leaving any oily/creamy residue on the skin. It doesnt strip the natural oils of the skin or dry it out but keeps the skin soft and supple. It refreshes the skin, keeps it oil free and also doesnt cause acne. It dissolves readily on coming in contact with water and hence has to be kept in a draining soapdish away from moisture/water when not in use. That being said, with proper storage, a single bar of soap lasts a little over a month with once daily use. Also, this soap, much like other The Body ...
Scientists in the UK are warning that the incidence of peanut allergies is increasing, and that it might be caused by early exposure to peanuts or peanut oil for children with eczema. By linking the fact that 90% of children with peanut allergies had previously experienced eczema, researchers have speculated that exposing peanuts to broken skin may actually spark off the allergy.
... seed storage proteins of grasses and cereals belong to the eponymous prolamin superfamily which also includes plant albumins(2S ... "Germins and germin-like proteins: Plant do-all proteins. But what do they do exactly?". Plant Physiology and Biochemistry. 39: ... but involved in plant responses to biotic and abiotic stress. "G-OXOs and GLPs are plant do-all proteins." Germin of the "true ... 400 plant species are susceptible including stone fruits. Thomas Burr Osborne at the end of the 19th century was the first ...
Bruix M, Santoro J, Rico M, Gimenez-Gallego G, Pantoja-Uceda D (2003). "Solution structure of RicC3, a 2S albumin storage ... Plant lipid transfer proteins, also known as plant LTPs or PLTPs, are a group of highly-conserved proteins of about 9kDa found ... Plant lipid transfer proteins share the same structural domain with seed storage proteins and trypsin-alpha amylase inhibitors ... LTPs in plants may be involved in: cutin biosynthesis surface wax formation mitochondrial growth pathogen defense reactions ...
... bonds in 2S albumin from plant seeds This enzyme is present in barley grain and other plants. Runeberg-Roos, P.; Törmäkangas, K ... A plant aspartic proteinase resembling mammalian cathepsin D". Eur. J. Biochem. 202 (3): 1021-1027. doi:10.1111/j.1432- ... "Structure and possible function of aspartic proteinases in barley and other plants". Adv. Exp. Med. Biol. Advances in ...
Soybeans contain a small but newly very significant 2S Albumin storage protein. Legume proteins, such as soy and pulses, belong ... However, Julian's plant must have also been the source of the "soy protein isolate" which Ford's Robert Boyer and Frank Calvert ... "2S Albumin Storage Proteins: What Makes them Food Allergens?". Open Biochem J. 2: 16-28. 2008. doi:10.2174/1874091X00802010016 ... The plant's eventual daily output of 40 tons of soy protein isolate made the Soya Products Division into Glidden's most ...
Peanuts, for instance, contain 20% 2S albumin but only 6% 7S globulin and 74% 11S.[35] It is the high 2S albumin and low 7S ... Like most plants, soybeans grow in distinct morphological stages as they develop from seeds into fully mature plant. ... Soybeans contain a small but significant 2S storage protein.[36][37][38] 2S albumin are grouped in the prolamin superfamily.[39 ... 2S albumins form a major group of homologous storage proteins in many dicot species and in some monocots but not in grasses ( ...
Ben de Lumen at the University of California-Berkeley identified the peptide as a subunit of the cotyledon-specific 2S albumin ... AF005030)" in "The Electronic Plant Gene Register". Plant Physiology. 114 (4): 1567-9. 1997. doi:10.1104/pp.114.4.1567. PMC ...
En groten Deel dorvun is dat 2S-Albumin.[1] Ok in de Butenschaal vun't Weetkoorn is Albumin in Form vun Leukosin binnen. Dat is ... P. R. Shewry, J. A. Napier, A. S. Tatham: Seed storage proteins: structures and biosynthesis. In: The Plant cell. Band 7, ... Albumin gifft dat in Melk (Lactalbumin) as ok in Höhner-Eiwitt (Ovalbumin). Homologen vun Albumin sünd in all Chordadeerter ... In de Forschung warrt Rinneralbumin (BSA, vun engl. bovine serum albumin) vör allen in de Immunologie insett, üm ünner annern ...
Ethylene serves as a hormone in plants. It acts at trace levels throughout the life of the plant by stimulating or regulating ... Additionally, H 2S reacts with intracellular S-nitrosothiols to form the smallest S-nitrosothiol (HSNO), and a role of hydrogen ... Sampath V, Zhao XJ, Caughey WS (Apr 27, 2001). "Anesthetic-like interactions of nitric oxide with albumin and hemeproteins. A ... It wasn't until 1934 that Gane reported that plants synthesize ethylene. In 1935, Crocker proposed that ethylene was the plant ...
The allergenicity of 2S plant albumins2011In: Clinical and Experimental Allergy, ISSN 0954-7894, E-ISSN 1365-2222, Vol. 41, no ... Mustard plants belong to the Brassicaceae/Cruciferae family. Mustard is present as an ingredient in different foods, sauces and ...
The allergenicity of 2S plant albumins2011In: Clinical and Experimental Allergy, ISSN 0954-7894, E-ISSN 1365-2222, Vol. 41, no ...
Bruix M, Santoro J, Rico M, Gimenez-Gallego G, Pantoja-Uceda D (2003). "Solution structure of RicC3, a 2S albumin storage ... Plant lipid transfer proteins, also known as plant LTPs or PLTPs, are a group of highly-conserved proteins of about 9kDa found ... Plant lipid transfer proteins share the same structural domain with seed storage proteins and trypsin-alpha amylase inhibitors ... LTPs in plants may be involved in: cutin biosynthesis surface wax formation mitochondrial growth pathogen defense reactions ...
... the present invention are produced in transgenic plants and then administered through the consumption of the transgenic plant. ... In such plants such as but not limited to Brassica napus (canola), expression vectors including the 2S albumin promoter and ... This term includes, without limitation, whole plants, plant cells, plant organs, plant seeds, protoplasts, callus, cell ... Ann De Clercq et al., Expression and Processing of an Arabidopsis 2S Albumin in Transgenic Tobacco, Plant Physiol. (1990) vol. ...
The 2S albumin large chain antibody (Scarafoni et al., 2001) was used in a 1:300 dilution, and it was kindly donated by A. ... Your Name) has sent you a message from Plant Cell Message Body (Your Name) thought you would like to see the Plant Cell web ... 2005). Autophagic recycling: Lessons from yeast help define the process in plants. Curr. Opin. Plant Biol. 8: 165-173. ... 2004). Processing of ATG8s, ubiquitin-like proteins, and their deconjugation by ATG4s are essential for plant autophagy. Plant ...
The compositions comprise genetically modified organisms, including fungi, yeast, bacterial and plant organisms that have been ... 1990) Plant Cell 2(6):533-46), 2S albumin (Saalbach et al. (1996) Plant Physiol 112(3):975-85), aleurain (Holwerda et al., 1992 ... Transformation of plant cells can be accomplished in similar fashion. By "plant" is intended whole plants, plant organs (e.g., ... "Transgenic plants" or "transformed plants" or "stably transformed" plants or cells or tissues refer to plants that have ...
Differential Polarization of Immune Responses by Plant 2S Seed Albumins, Ber e 1, and SFA8 Dorothy E. Kean, Helen S. Goodridge ...
... seed storage proteins of grasses and cereals belong to the eponymous prolamin superfamily which also includes plant albumins(2S ... "Germins and germin-like proteins: Plant do-all proteins. But what do they do exactly?". Plant Physiology and Biochemistry. 39: ... but involved in plant responses to biotic and abiotic stress. "G-OXOs and GLPs are plant do-all proteins." Germin of the "true ... 400 plant species are susceptible including stone fruits. Thomas Burr Osborne at the end of the 19th century was the first ...
The allergenicity of 2S plant albumins2011In: Clinical and Experimental Allergy, ISSN 0954-7894, E-ISSN 1365-2222, Vol. 41, no ... The ins and outs of stable isotopes in plants.2007In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 174, no 3, 473- ... Ber e 1 was also one of the first food allergens to be unintentionally transferred from one plant to another and was involved ... A comparison of the D isotopomer abundances of soluble sugars of annual plants and of trees, and of tree-ring cellulose shows ...
Sensitization to 2S albumin proteins such as Jug r 1 is known to be associated with systemic food reactions.7,8,18,19 ... Allergies to plant-derived foods can occur as the result of sensitization to relatively stable proteins, such as the seed ... Ana o 3, an important cashew nut (Anacardium occidentale L.) allergen of the 2S albumin family. J Allergy Clin Immunol. 2005 ... Sensitization to the storage protein Jug r 1 (2S albumin) indicates a primary walnut allergy.6,7 ...
... and also 2S albumins found in many tree nuts-including Brazil nuts, English walnuts-and also in seeds, including yellow mustard ... Virtually all foods-including both plant and animal foods-contain lectins. Among plant foods, the best-studied sources of ... Plant-Based Way of Eating *Organic Foods Healthy Cooking. *Recipe of the Week *Nutrient-Rich Cooking *300 Recipes *Cooking with ... These are some common allergens found in plant foods: *prolamins (a very large family of allergens that includes seed storage ...
... anti-2S albumin, and anti-GFP antibodies to detect 12S globulin subunits and 12S globulin precursors (D), 2S albumin and 2S ... 2S albumin and 12S globulin. In total extracts derived from seeds, precursor proteins of both 2S albumin and 12S globulin ... M1 plants were then self-pollinated, and seeds of approximately 3,500 M1 plants were collected in pools containing five plants ... fyve1-1 mutants are impaired in 12S globulin and 2S albumin processing. A, Seeds of the wild type (WT; left) together with GFP- ...
1993). Vesicle transport and processing of the precursor to 2S albumin in pumpkin. Plant J. 4, 790-800. ... Your Name) has sent you a message from Plant Cell Message Body (Your Name) thought you would like to see the Plant Cell web ... Plant Material. Pea (Pisum sativum var Exzellent) plants were grown hydroponically. Cotyledons with 8- and 9-mm-long axis ... 1997). The syntaxin homolog AtPEP12p resides on a late post-Golgi compartment in plants. Plant Cell 9, 571-582. ...
Plant Molecular Biology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic ... Modified 2S albumins with improved tryptophan content are correctly expressed in transgenic tobacco plants ... Expression and processing of an Arabidopsis 2S albumin in transgenic tobacco. De Clercq, A.; Vandewiele, M.; De Rycke, R.; Van ... Enkephalins produced in transgenic plants using modified 2S seed storage proteins. Vandekerckhove, J.; Van Damme, J.; Van ...
Thus, the number and function of miRNAs must have significantly expanded during the evolution of early land plants. As we have ... Frequently, not only single miRNAs but whole families of closely related miRNAs have been found in animals and plants. Some ... the coupled maturation of two miRNAs from a shared precursor has not been previously identified in plants. ... families are widely conserved among different plant taxa. Hence, it is evident that these conserved miRNAs are of ancient ...
Plant Molecular Biology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic ... Vesicle transport and processing of the precursor to 2S albumin in pumpkin ... Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology. ISSN. 0167-4412. eISSN. 1573-5028. D.O.I.. 10.1023/A: ... Program in Plant Physiology, School of Molecular Sciences, Washington State University, Pullman, WA 99164- ...
This is a new addition to the group which now consists of the trypsin/alpha-amylase inhibitors, 2S seed storage albumins, and ... Apparently, the four-helix conformation has been a successful vehicle in plant evolution for providing protection from ... trypsin/alpha-amylase inhibitor family is evolutionarily related to the family of nonspecific lipid-transfer proteins of plants ...
Plant nonspecific lipid transfer proteins, 2S albumins, proteinase inhibitors, and puroindolines act synergistically with ... Both Plant Sentinels and Foot Soldiers Need to Know the Enemy Message Subject (Your Name) has sent you a message from Plant ... 1994) The physiology and molecular biology of plant 1,3-β-d-glucanases and 1,3;1,4-β-d-glucanases. Crit Rev Plant Sci 13:325- ... 1999) Plants have a sensitive perception system for the most conserved domain of bacterial flagellin. Plant J 18:265-276. ...
Walnut allergens identified include Jug r 1 (walnut 2S albumin), Jug 3 r (vicillin-like protein), and Jug 3 r (a 9-kd lipid ... The PLANTS Database ( http://plants.usda.gov, 28 Oct 2007 ). National Plant Data Center, Baton Rouge, LA 70874-4490 USA.. 2. ... Lipid transfer proteins and 2S albumins as allergens. Allergy . 2001;56(suppl 67):45-47.. 36. Pastorello EA, Farioli L, ... Pharmacognosy, Phytochemistry, Medicinal Plants . Paris; New York: Lavoisier Publishing; 1995:348.. 5. DAmelio FS. Botanicals ...
Altogether, these results suggest that Mo-CBP3 might be involved in plant defense mechanisms and could be used as potential ... antifungal agent for controlling fungal pathogens in plants. ... Is a Member of the 2S Albumin Family. *José Ednésio da Cruz ... Plant antifungal proteins and their applications in agriculture. *Juan Yan, Su-su Yuan, Luan-luan Jiang, Xiu-juan Ye, T. B. Ng ... A novel chitin-binding protein from Moringa oleifera seed with potential for plant disease control.. @article{Gifoni2012ANC, ...
Enhanced methionine and cysteine levels in transgenic rice seeds by the accumulation of sesame 2S albumin. Biosci Biotechnol ... Plant and seed nutrient uptake in common bean in seven organic and conventional production systems. Can J Plant Sci (2011) 91: ... Increased sulfur amino acids in soybean plants overexpressing the maize 15 kDa zein protein. In Vitro Cell Dev Biol Plant (2001 ... Recent advances in the ecological chemistry of plant terpenoids. Ecol Chem Biochem Plant Terpenoids (1991) 6:399-426. ...
The vacuolar transport of aleurain-GFP and 2S albumin-GFP fusions is mediated by the same pre-vacuolar compartments in tobacco ... Plant Cell Physiol. 2008 Feb;49(2):142-56. doi: 10.1093/pcp/pcn006. Epub 2008 Jan 25. Erratum in: Plant Cell Physiol. 2008 Apr; ... Sorting of plant vacuolar proteins is initiated in the ER.. Niemes S, Labs M, Scheuring D, Krueger F, Langhans M, Jesenofsky B ... Plant J. 2010 May 1;62(4):601-14. doi: 10.1111/j.1365-313X.2010.04171.x. Epub 2010 Feb 10. ...
BMC Plant Biology 9 (1), 133, 2009. 81. 2009. Transport of ricin and 2S albumin precursors to the storage vacuoles of Ricinus ... Overexpression of a plant reticulon remodels the lumen of the cortical endoplasmic reticulum but does not perturb protein ... David G. RobinsonProfessor for Plant Cell Biology, University of HeidelbergVerified email at urz.uni-heidelberg.de ... Protein quality control along the route to the plant vacuole.. E Pedrazzini, G Giovinazzo, A Bielli, M de Virgilio, L Frigerio ...
Walnut allergens identified include Jug r 1 (walnut 2S albumin), Jug 3 r (vicillin-like protein), and Jug 3 r (a 9-kd lipid ... The PLANTS Database (http://plants.usda.gov, April 2017). National Plant Data Team, Greensboro, NC 27401-4901 USA. ... Lipid transfer proteins and 2S albumins as allergens. Allergy. 2001;56(suppl 67):45-47.11298008. ... Pharmacognosy, Phytochemistry, Medicinal Plants. Paris; New York: Lavoisier Publishing; 1995:348.. 6. DAmelio FS. Botanicals: ...
The sequence of Lec2SA was similar to that of 2S albumins from different plants, such as Brazil nut and castor beans (26). The ... The study concluded that treatment with plant hormones induced a more intense cutaneous response (25).. A 2S storage albumin, ... Oguri S, Kamoshida M, Nagata Y, Momonoki YS, Kamimura H. Characterization and sequence of tomato 2S seed albumin: a storage ... Plant Biotech J 2006;4(2):231-42 *Lorenz Y, Enrique E, Lequynh L, Fotisch K, Retzek M, Biemelt S, Sonnewald U, Vieths S, ...
  • This is a new addition to the group which now consists of the trypsin/alpha-amylase inhibitors, 2S seed storage albumins, and the lipid-transfer family. (rcsb.org)
  • Apparently, the four-helix conformation has been a successful vehicle in plant evolution for providing protection from predators, food for the embryo, and lipid transfer. (rcsb.org)
  • Based on their molecular masses, plant nsLTPs were first separated into two types: type I (9 kDa) and type II (7 kDa) that are distinct both in terms of primary sequence identity (less than 30%) and lipid transfer efficiency [ 3 ]. (biomedcentral.com)
  • Because they have been shown to transfer lipid molecules between membranes in vitro , plant nsLTPs were first suggested to be involved in membrane biogenesis [ 1 ]. (biomedcentral.com)
  • Croy, Ronald 2004-10-03 00:00:00 Napin is a 2S storage protein found in the seeds of oilseed rape (Brassica napus L.) and related species. (deepdyve.com)
  • Here, we attempted to test the structural stability of the methionine-enhanced phaseolin by introducing phaseolin cDNA to tobacco via Agrobacterium tumefaciens -mediated transformation of leaf disks, regenerating transgenic tobacco plants, and examining the accumulation of phaseolin protein in mature transgenic tobacco seeds. (scirp.org)
  • Castor meal is a concentrated residue matter produced after extraction of oil from castor seeds.Its application is so restricted due to ricin and poor molecular biochemical knowledge about the material.A surprising breakthrough has been made by the discovery of galactinol, a carbohydrate derivative associated with raffinose metabolism in plants to an appreciable extent on mass basis. (castoroil.in)
  • Results of this study clearly indicate that proteases play a vital role in castor seed germination as we normally observe with plant seed germination and a comprehensive profiling of proteases would enable biologists to exploit them as candidate markers for the selection of seeds with effective germination and productive stand in the field. (castoroil.in)
  • Do Nascimento V.V., Castro H.C., Abreu P.A., Oliveira A.E., Fernandez J.H., Araujo Jda S. & Machado O.L. 2011: In silico structural characteristics and α-amylase inhibitory properties of Ric c 1 and Ric c 3, allergenic 2S albumins from Ricinus communis seeds. (eje.cz)
  • Asp- bonds in 2S albumin from plant seeds. (cbrc.jp)
  • Like most plants, soybeans grow in distinct morphological stages as they develop from seeds into fully mature plant. (wikipedia.org)
  • The easiest way to germinate tobacco seeds Simple techniques for producing the largest tobacco plants Hands free maintenance allowing you to set it and forget it The very best time for harvesting Drying and curing for maximum flavour and quality The different types of tobacco available to you. (europeanmedical.info)
  • How to choose the best seeds for the best plants. (europeanmedical.info)
  • The introduction of a phytoene synthase gene with an RNAi cassette directed to suppress the storage protein 2S albumin resulted in seeds with an 11-24 % elevation in overall protein. (bireme.br)
  • Stacking an RNAi to suppress the major 2S storage protein resulted in seeds that contain elevated protein and slight decrease in oil and carbohydrate amounts showing that Camelina rebalances its proteome within an enlarged protein content genotype. (bireme.br)
  • 7 ] transformed the sesame 2S albumin gene into rice and produced "sesame nutrition rice", and the methionine content of the seeds reached 0.40% in the T 2 generation rice lines, which represented a 38% increase relative to that of the control rice lines. (biomedcentral.com)
  • 2S-Albumine falten sich wie alle Mitglieder der Prolamin-Superfamilie in ein kompaktes Bündel aus vier bis fünf α-Helices, die durch vier bis fünf konservierte. (springer.com)
  • With the exception of Peanut, 2S albumins could therefore account for the IgE-binding cross-reactivity observed among some other dietary nuts, e.g. (phadia.com)
  • We have used a discriminative seeding DNA motif discovery algorithm for an in-depth analysis of 54 seed storage protein (SSP) gene promoters from three plant families, namely Brassicaceae (mustards), Fabaceae (legumes) and Poaceae (grasses) using backgrounds based on complete sets of promoters from a representative species in each family, namely Arabidopsis ( Arabidopsis thaliana (L.) Heynh. (biomedcentral.com)
  • The genomes of higher plants, including Arabidopsis thaliana , Glycine max (soybean), Oryza sativa (rice), and Zea mays (maize), encode approximately 10 to 20 members of the PDI family, which show wide variation in the organization of their thioredoxin (TRX)-fold domains [ 4 , 5 ]. (hindawi.com)
  • Foliar diseases of sesame such as Alternaria leaf blight may cause significant yield losses, with reductions in plant health and seed quality. (ppjonline.org)
  • The pathogenicity and virulence of these isolates of Alternaria alternata was confirmed in inoculations of sesame plants resulting in typical symptoms of leaf blight disease. (ppjonline.org)
  • Sesame ( Sesamum indicum ) is an oil seed plant of the family of Pedaliaceae, originating in India and cultivated in Africa, Asia, the Balkans, the Middle East, Latin America and USA. (manchester.ac.uk)
  • The chimeric genes have been expressed in tobacco plants under the control of the seed-specific napA gene promoter. (deepdyve.com)
  • This phenomenon, which is known as posttranscriptional gene silencing, was first identified in C. elegans [ 4 ], but was soon shown to be a regulatory mechanism in plants and animals. (biomedcentral.com)
  • Part of plant defense strategy includes an active offense against invading microbes using an array of antimicrobial gene products. (plantphysiol.org)
  • Specific host-pathogen interaction models describing induced defense responses in plants have been greatly influenced in recent years by the gene-for-gene interactions originally reported by Flor (1956) . (plantphysiol.org)
  • In these specific host-pathogen interactions, resistance to a particular pathogen is conditional on the presence of a specific Avr (avirulence) gene of the pathogen and a specific R (resistance) gene (usually a single dominant gene) in the plant host. (plantphysiol.org)
  • We now understand that the Avr gene system of potential plant pathogens directly or indirectly provides a biochemical target for a plant surveillance system in which the R gene plays a central role. (plantphysiol.org)
  • A novel chemical-induced gene regulatory system for plants consisting of two molecular components is described. (usda.gov)
  • It has gained particular interest as in the early 1990's it was considered to transfer the gene coding for the Brazil nut 2S albumin by transgenic techniques to soybean in order to improve its nutritional value for animal feed which however never reached the market due to the allergenic nature of the Brazil nut 2S albumin [ 3 ]. (biomedcentral.com)
  • or (2) expression of a recombinant or mutant AtSR1 sequence (or AtSR1 gene ortholog or homolog sequence) encoding a modified AtSR1, or AtSR1 ortholog or homolog protein, in a plant or plant cell, wherein said protein modification reduces or eliminates the calmodulin-binding activity of the respective AtSR1 or AtSR1 ortholog or homolog protein. (patentsencyclopedia.com)
  • Furthermore, transgenic plants overexpressing a TF of interest can present pleiotropic phenotypes and/or result in a high number of indirect gene expression changes. (biomedcentral.com)
  • Agroinfiltration is a simple plant biology method that allows transient gene expression. (biomedcentral.com)
  • Evidence of the presence of a variant of the TATA-box is found in the SSP gene promoters from the three plant families. (biomedcentral.com)
  • Such plants exhibit HR-like spontaneous lesions and mutations in the bO gene that affect the proton channeling capacity abolish this phenotype. (europeanmedical.info)
  • We characterized the biological and molecular functions of AtC3H17, a unique Arabidopsis gene encoding a non-tandem CCCH zinc finger protein, in plant development. (omicsdi.org)
  • In the present study, the P. japonicus βAS gene was transformed into the rice cultivar 'Taijing 9' using an Agrobacterium -mediated approach, resulting in 68 rice transgenic plants of the T 0 generation. (biomedcentral.com)
  • Approximately 1-2 copies of the βAS gene were detected in transgenic rice plants. (biomedcentral.com)
  • Our model shows a typical structural features of 2S albumins such as a four helical segments forming a compact hydrophobic core organised in a "up and down" architecture. (unipd.it)
  • Exposure to redox-cycling drugs causes one-electron oxidation of SoxR's [2Fe-2S] centers, and the oxidized protein facilitates soxS transcription by mediating a structural change in the promoter DNA that stimulates initiation by RNA polymerase ( 14 , 15 , 21 , 22 , 28 ). (asm.org)
  • Besides its biological relevance as a model to study plant development, cell differentiation, and programmed cell death, the cereal endosperm is very important in terms of its nutritional value. (plantcell.org)
  • DNA sequences both natural and synthetic encoding for the expression of immunogenic agents which are capable of causing an immune response in animals when fed in edible plants, plant tissues, or derived plant materials are constructed, and plants transformed for stable or transient expression in plant cells. (freepatentsonline.com)
  • The present invention furthermore relates to nucleic acid sequences which code for a protein which confers an increased pathogen resistance in plants. (patentsencyclopedia.com)
  • 4. A recombinant nucleic acid molecule, comprising the following elements in 5'-3' orientation:regulatory sequences of a promoter which is active in plant cells,the nucleic acid sequence according to claim 1 in operable linkage thereto, optionally, regulatory sequences which may act as transcription, termination and/or polyadenylation signals in the plant cell, in operable linkage thereto. (patentsencyclopedia.com)
  • 14. A transgenic plant cell, comprising the nucleic acid sequence as claimed in claim 1 or a recombinant nucleic acid molecule comprising in 5'-3' orientation regulatory sequences of a promoter which is active in plant cells and the nucleic acid sequence according to claim 1 in operable linkage thereto. (patentsencyclopedia.com)
  • Collections of experimentally characterized plant cis -regulatory elements sequences such as the PLACE database [ 5 ] nevertheless remain an invaluable resource e . g . for annotating motifs discovered in sequences that have not been characterized experimentally. (biomedcentral.com)
  • 400 plant species are susceptible including stone fruits. (wikipedia.org)
  • Thus, the analysis of an early-branching species provides a window into the early evolution of fundamental regulatory processes in plants. (biomedcentral.com)
  • Jbilou R., Amri H., Bouayad N., Ghailani N., Ennabili A. & Sayah F. 2008: Insecticidal effects of extracts of seven plant species on larval development, α-amylase activity and offspring production of Tribolium castaneum (Herbst) (Insecta: Coleoptera: Tenebrionidae). (eje.cz)
  • People with tree nut allergies also often suffer from reactions triggered by a number of different types of nuts, even though they do not come from closely related plant species. (manchester.ac.uk)
  • This included the identification of target genes of a TF not normally expressed in leaves, and targets of TFs from other plant species. (biomedcentral.com)
  • Infiltration-RNAseq can be easily adapted to other plant species where agroinfiltration protocols have been optimised. (biomedcentral.com)
  • About 90 species of plant pollen have been identified as aeroallergens. (medicinalplantsarchive.us)
  • Research materials are represented by the lupin collection of the N.I. Vavilov Institute of Plant Industry (VIR) storing now more than 2500 accessions of different lupin species collected by numerous expeditions over the globe. (blogspot.com)
  • Many years of observing plants sown in various regions have enabled us to find out new regularities in the variability of characters depending on genetic features of the species and accessions, and also on ecological and agronomic conditions of their growth. (blogspot.com)
  • Key genes that differentiated species responses were linked to photoprotection/redox balance, phytohormone/signalling, primary photosynthesis/cellular metabolism and secondary metabolism based on plant metabolic pathway network analysis. (biomedcentral.com)
  • B. Nüsse und Hülsenfrüchte) wurden drei Gruppen als Allergene beschrieben: die 2S-Albumine, die 7S-Globuline und die 11S-Globuline. (springer.com)
  • Die Sensibilisierungen gegen die Allergene aus der Proteinfamilie 2S Albumine, nsLTPs und Bet v 1-Homologen waren häufiger in der Gruppe der erdnuss- und baumnussallergischen Patienten nachweisbar als in der Gruppe der erdnussallergischen Patienten. (meduniwien.ac.at)
  • Die Strategie zur effizienten Präparation großer Mengen Protein basiert auf der Anwendung spezieller E. coli Stämme mit oxidativem Cytoplasma und eignet sich zur Expression nativ gefalteter und disulfidverbrückter rekombinanter Erdnussallergene vom 2S Albumintyp und mit hoher Wahrscheinlichkeit weiterer Mitglieder dieser Proteinfamilie. (uni-bayreuth.de)
  • Franco O.L., Rigden D.J., Melo F.R. & Grossi-de-Sa M.F. 2002: Plant α-amylase inhibitors and their interaction with insect α-amylase. (eje.cz)
  • 1 Department of Plant Pathology and Crop Physiology, Louisiana State University and LSU Agricultural Center, Baton Rouge, LA, USA . (scirp.org)
  • Plant Physiology, 61, 918-923. (scirp.org)
  • Enno Krebbers , Lydia Herdies , Ann De Clercq , Jef Seurinck , Jan Leemans , Jozef Van Damme UGent , Magdalena Segura , Godelieve Gheysen UGent , Marc Van Montagu UGent and Joël Vandekerckhove ( 1988 ) PLANT PHYSIOLOGY . (ugent.be)
  • K DHONDT , J VANDAMME , C VANDENBOSSCHE , S LEEJEERAJUMNEAN , R DERYCKE , J DERKSEN , Joël Vandekerckhove and E KREBBERS ( 1993 ) PLANT PHYSIOLOGY . (ugent.be)
  • Plant Physiology 102 (2): 425-433. (ugent.be)
  • It was suggested that "germin" , {first found and only known to occur in the "true cereals": barley, corn, oat, rice, and wheat} a plant enzyme, oxalate oxidase 'one-very-tough-little- protein' as such an ancestor. (wikipedia.org)
  • A complex array of interactions between plants and microbes has evolved that reflects both the nutrient acquisition strategies of microbes and defense strategies of plants. (plantphysiol.org)
  • Plant defense systems are also induced by microbial products in compatible (resulting in disease) and incompatible (failure to result in disease) plant-microbe interactions. (plantphysiol.org)
  • Obwohl Erdnuss und Baumnüsse zu unterschiedlichen taxonomischen Pflanzengruppen gehören, sind die Hauptallergene Angehörige derselben Proteinfamilien, wie die 2S Albumine, Viciline, Legumine, nicht spezifischen Lipidtransferproteinen (nsLTPs) und Bet v 1-Homologen. (meduniwien.ac.at)
  • Until such time as specific physiological functions are defined, it seems relevant to categorize plant nsLTPs on the basis of sequence similarity and/or phylogenetic clustering. (biomedcentral.com)
  • All known plant nsLTPs are synthesized as precursors with a N-terminal signal peptide. (biomedcentral.com)
  • The identification of genes and transcriptional networks regulated by a TF often requires stable transformation and expression changes in plant cells. (biomedcentral.com)
  • Expression of these genes depends on the production of the benzochromanequinone antibiotic actinorhodin and requires intact [2Fe-2S] clusters in SoxR. (asm.org)
  • It is still uncertain whether plant surveillance systems utilize molecular pattern recognition to identify pathogen challenges. (plantphysiol.org)
  • Chenopodium 2S albumin is, however, antigenically unrelated to the low-molecular-weight albumins of dicots belonging to other families (19). (phadia.com)
  • The scope of environmental conditions tolerated by the genus Eucalyptus , and the diversity of physiological responses to the availability of water detected among contrasting taxonomic groups [ 5 ] suggests both isohydric and anisohydric approaches to plant growth and survival are prevalent within the Genus. (biomedcentral.com)
  • The compositions comprise genetically modified organisms, including fungi, yeast, bacterial and plant organisms that have been engineered to express heterologous genes involved in chitin and chitosan synthesis. (freepatentsonline.com)
  • The experiment was designed to identify differentially expressed genes between WT and AtC3H17-overexpressing transgenic plants (AtC3H17 OXs) grown for 14 days under SD growth conditions. (omicsdi.org)
  • 2011). Plant cystatins (phytocystatins) are assumed to regulate endogenous cysteine protease activities in response to biotic and abiotic stresses during seed development and germination, when stored reserves are mobilized for embryogenesis and seedling growth (Abe et al. (johnnyfavourit.com)