Antibodies produced by a single clone of cells.
A gland in males that surrounds the neck of the URINARY BLADDER and the URETHRA. It secretes a substance that liquefies coagulated semen. It is situated in the pelvic cavity behind the lower part of the PUBIC SYMPHYSIS, above the deep layer of the triangular ligament, and rests upon the RECTUM.
Sites on an antigen that interact with specific antibodies.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Chemicals administered to patients in order to diagnose or study the pathology of medical conditions, diseases, or syndromes.
A collection of cloned peptides, or chemically synthesized peptides, frequently consisting of all possible combinations of amino acids making up an n-amino acid peptide.
Temperate bacteriophage of the genus INOVIRUS which infects enterobacteria, especially E. coli. It is a filamentous phage consisting of single-stranded DNA and is circularly permuted.
Techniques utilizing cells that express RECOMBINANT FUSION PROTEINS engineered to translocate through the CELL MEMBRANE and remain attached to the outside of the cell.
Viruses whose hosts are bacterial cells.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Partial immunoglobulin molecules resulting from selective cleavage by proteolytic enzymes or generated through PROTEIN ENGINEERING techniques.
A form of antibodies consisting only of the variable regions of the heavy and light chains (FV FRAGMENTS), connected by a small linker peptide. They are less immunogenic than complete immunoglobulin and thus have potential therapeutic use.
Literary and oral genre expressing meaning via symbolism and following formal or informal patterns.
A plant family of the order Pinales, class Pinopsida, division Coniferophyta (conifers). They are mainly resinous, aromatic evergreen trees.
DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.
Vaccines used to prevent infection by MUMPS VIRUS. Best known is the live attenuated virus vaccine of chick embryo origin, used for routine immunization of children and for immunization of adolescents and adults who have not had mumps or been immunized with live mumps vaccine. Children are usually immunized with measles-mumps-rubella combination vaccine.
A federal area located between Maryland and Virginia on the Potomac river; it is coextensive with Washington, D.C., which is the capital of the United States.
The type species of RUBULAVIRUS that causes an acute infectious disease in humans, affecting mainly children. Transmission occurs by droplet infection.
Peptide sequences, generated by iterative rounds of SELEX APTAMER TECHNIQUE, that bind to a target molecule specifically and with high affinity.
Nucleotide sequences, generated by iterative rounds of SELEX APTAMER TECHNIQUE, that bind to a target molecule specifically and with high affinity.
A method of generating a large library of randomized nucleotides and selecting NUCLEOTIDE APTAMERS by iterative rounds of in vitro selection. A modified procedure substitutes AMINO ACIDS in place of NUCLEOTIDES to make PEPTIDE APTAMERS.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
A signal transducing adaptor protein and tumor suppressor protein. It forms a complex with activated RECEPTOR-REGULATED SMAD PROTEINS. The complex then translocates to the CELL NUCLEUS and regulates GENETIC TRANSCRIPTION of target GENES.
A receptor-regulated smad protein that undergoes PHOSPHORYLATION by ACTIVIN RECEPTORS, TYPE I. Activated Smad3 can bind directly to DNA, and it regulates TRANSFORMING GROWTH FACTOR BETA and ACTIVIN signaling.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A semisynthetic derivative of PODOPHYLLOTOXIN that exhibits antitumor activity. Etoposide inhibits DNA synthesis by forming a complex with topoisomerase II and DNA. This complex induces breaks in double stranded DNA and prevents repair by topoisomerase II binding. Accumulated breaks in DNA prevent entry into the mitotic phase of cell division, and lead to cell death. Etoposide acts primarily in the G2 and S phases of the cell cycle.
A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.
A group of PROTEIN-SERINE-THREONINE KINASES which activate critical signaling cascades in double strand breaks, APOPTOSIS, and GENOTOXIC STRESS such as ionizing ultraviolet A light, thereby acting as a DNA damage sensor. These proteins play a role in a wide range of signaling mechanisms in cell cycle control.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Activating transcription factors of the MADS family which bind a specific sequence element (MEF2 element) in many muscle-specific genes and are involved in skeletal and cardiac myogenesis, neuronal differentiation and survival/apoptosis.
The artificial induction of GENE SILENCING by the use of RNA INTERFERENCE to reduce the expression of a specific gene. It includes the use of DOUBLE-STRANDED RNA, such as SMALL INTERFERING RNA and RNA containing HAIRPIN LOOP SEQUENCE, and ANTI-SENSE OLIGONUCLEOTIDES.
A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.
A DNA library for any protein or small peptide of interest can be synthesized by solid-phase synthesis followed by PCR ... They allow a library size as large as 10^15 different members. The large library size increases the probability to select very ... For example, the library size for phage and bacterial display is limited to 1-10 × 10^9 different members. The library size for ... The T7 promoter region allows large-scale in vitro T7 transcription to transcribe the DNA library into an mRNA library, which ...
"Affinity panning of a library of peptides displayed on bacteriophages reveals the binding specificity of BiP". Cell. 75 (4): ... Turku University Library: Ann. Univ. Turkuensis D 619. ISBN 951-29-2742-X. Retrieved October 9, 2012. Kober L, Zehe C, Bode J ( ... Urano F, Wang X, Bertolotti A, Zhang Y, Chung P, Harding HP, Ron D (January 2000). "Coupling of stress in the ER to activation ... Mori K, Ogawa N, Kawahara T, Yanagi H, Yura T (April 2000). "mRNA splicing-mediated C-terminal replacement of transcription ...
... via Snake Venomics and Combinatorial Peptide Ligand Library Approaches". Journal of Proteome Research. 8 (6): 3055-67. doi: ... Gutiérrez, J; Rucavado, A (2000). "Snake venom metalloproteinases:Their role in the pathogenesis of local tissue damage". ...
... as Detected via Combinatorial Peptide Ligand Libraries". Journal of Proteome Research. 10 (5): 2684-2686. doi:10.1021/pr2001447 ... 2000). For God, Country and Coca-Cola: The Definitive History of the Great American Soft Drink and the Company that Makes it ( ...
Vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase activating polypeptide (PACAP) are homologous peptides that ... National Library of Medicine. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library ... 2001). "Expression of vasoactive intestinal peptide (VIP) receptors in human uterus". Peptides. 21 (9): 1383-8. doi:10.1016/ ... "Vasoactive intestinal peptide/pituitary adenylate cyclase-activating peptide receptor subtypes in human tumors and their ...
ISBN 3-527-29869-X. G. Jung (ed.): Combinatorial Peptide and Nonpeptide Libraries, A Handbook. Wiley-VCH, Weinheim. 1996. ISBN ... The sequence motifs of MHC-bound natural peptide libraries (major histocompatibility complex) has been determined for the first ... peptide libraries, complete B- and T-cell epitope mapping of viral proteins, immunobiochemistry. ... pool sequencing of synthetic and natural peptide libraries". Anal Biochem. 212 (1): 212-220. doi:10.1006/abio.1993.1314. PMID ...
"Past and future perspectives of synthetic peptide libraries". Current Protein & Peptide Science 9 (5): 447-67. doi:10.2174/ ... An efficient peptide coupling additive". Journal of the American Chemical Society 115 (10): 4397-8. doi:10.1021/ja00063a082 . ... Hylin, John W. (1969). "Toxic peptides and amino acids in foods and feeds". Journal of Agricultural and Food Chemistry 17 (3): ... Rodnina MV, Beringer M, Wintermeyer W (Januari 2007). "How ribosomes make peptide bonds". Trends in Biochemical Sciences 32 (1 ...
"Determination of substrate specificity for peptide deformylase through the screening of a combinatorial peptide library". ... In enzymology, a peptide deformylase (EC 3.5.1.88) is an enzyme that catalyzes the chemical reaction formyl-L-methionyl peptide ... Pei D (2001). "Peptide deformylase: a target for novel antibiotics?". Emerging Therapeutic Targets. 5 (1): 23-40. doi:10.1517/ ... Chan MK, Gong W, Rajagopalan PT, Hao B, Tsai CM, Pei D (1997). "Crystal structure of the Escherichia coli peptide deformylase ...
National Library of Medicine. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library ... Prolactin-releasing peptide (PrRP) is a peptide hormone that in humans is encoded by the PRLH gene. PrRP stimulates prolactin ( ... This extract is more helpful.) Hinuma S, Onda H, Fujino M (1999). "The quest for novel bioactive peptides utilizing orphan ... 2003). "Expression of prolactin-releasing peptide and its receptor in the human adrenal glands and tumor tissues of ...
National Library of Medicine. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library ... "Entrez Gene: Formyl peptide receptor 1". Migeotte I, Communi D, Parmentier M (Dec 2006). "Formyl peptide receptors: a ... "Synthesis and use of a novel N-formyl peptide derivative to isolate a human N-formyl peptide receptor cDNA". Biochemical and ... "Synthesis and use of a novel N-formyl peptide derivative to isolate a human N-formyl peptide receptor cDNA". Biochemical and ...
All peptide sequences obtained from biopanning using combinatorial peptide libraries have been stored in a special freely ... Libraries of peptides and proteins displayed on filamentous phage. Methods in Enzymology. 1993. 217:228-257. ... Biopanning involves 4 major steps for peptide selection. The first step is to have phage display libraries prepared. This ... Mandecki W, Chen YC, and Grihalde N. A Mathematical Model for Biopanning (Affinity Selection) Using Peptide Libraries on ...
Gastrin-Releasing+Peptide at the US National Library of Medicine Medical Subject Headings (MeSH) Nosek, Thomas M. "Section 6/ ... Gastrin-releasing peptide is a regulatory human peptide that elicits gastrin release and regulates gastric acid secretion and ... 2003). "Expression of progastrin-releasing peptide and gastrin-releasing peptide receptor mRNA transcripts in tumor cells of ... National Library of Medicine. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library ...
Moon, Heejo; Lim, Hyun-Suk (2015-02-01). "Synthesis and screening of small-molecule α-helix mimetic libraries targeting protein ... Peptides with multiple, tandem staples are sometimes referred to as stitched peptides. Among other applications, peptide ... A stapled peptide is a short peptide, typically in an alpha-helical conformation, that is constrained by a synthetic brace (" ... Beta-peptide Druggability Non-proteinogenic amino acids Peptide synthesis Peptidomimetic Peptoid Douse, CH; Maas, SJ; Thomas, ...
Rapid selection and identification of functional CD8+ T cell epitopes from large peptide-coding libraries. Nat Comm, 2019. 10: ... Science, 2000. 287: 2185 Breakthrough of the Year (Science 290:2255). Venter, JC, Adams, MD, Meyers, EW, Li, PW, Mural, RJ, ...
Signal peptide peptidase-like 2B, also known as SPPL2B, is a human gene. This gene is a member of the signal peptide peptidase- ... National Library of Medicine. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library ... "Entrez Gene: SPPL2B signal peptide peptidase-like 2B". CS1 maint: discouraged parameter (link) Friedmann E, Hauben E, Maylandt ... 2005). "Consensus analysis of signal peptide peptidase and homologous human aspartic proteases reveals opposite topology of ...
This approach was also used in screening peptide libraries. The tethered peptide library was tested with a dissolved target ... In omission libraries a certain amino acid is missing from all peptides of the mixture. The figure shows the full library and ... If we deal with a non-peptide organic libraries library it is not as simple to determine the identity of the content of a bead ... Cwirla, S. E.; Peters, E. A.; Barrett, R. W.; Dower, W. J. (1990-08-01). "Peptides on phage: a vast library of peptides for ...
"T7 displayed peptides as targets for selecting peptide specific scFvs from M13 scFv display libraries". J. Immunol. Methods. ... Peptides are usually fused to the N-terminus of pVIII. Usually peptides that can be fused to pVIII are 6-8 amino acids long. ... A patent by George Pieczenik claiming priority from 1985 also describes the generation of peptide libraries. Like the two- ... In 1990, Jamie Scott and George Smith described creation of large random peptide libraries displayed on filamentous phage. ...
National Library of Medicine. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library ... Peptide deformylase, mitochondrial is an enzyme that in humans is encoded by the PDF gene. Period (gene) Timeless (gene) GRCh38 ... 2004). "An unusual peptide deformylase features in the human mitochondrial N-terminal methionine excision pathway". J. Biol. ... "Entrez Gene: PDF peptide deformylase (mitochondrial)". CS1 maint: discouraged parameter (link) Gerhard DS, Wagner L, Feingold ...
Peptide+Elongation+Factor+G at the US National Library of Medicine Medical Subject Headings (MeSH). ... The nascent peptide continues to fold and leaves the 70S ribosome, the mRNA, the deacylated tRNA (P site), and the Class I ... A Class I release factor (RF1 or RF2) binds to the stop codon, which induces hydrolysis of the tRNA-peptide bond in the P site ... In this process, the peptidyl transferase center (PTC) has catalyzed the formation of a peptide bond between amino acids, ...
Peptides. 31 (2): 257-62. doi:10.1016/j.peptides.2009.11.024. PMC 4043136. PMID 19961888. Arnes L, Hill JT, Gross S, Magnuson ... National Library of Medicine. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library ... Peptides. 26 (11): 2274-9. doi:10.1016/j.peptides.2005.04.025. PMID 16137788. S2CID 25619880. Jerlhag E, Egecioglu E, Dickson ... The hormone name is based on its role as a growth hormone-releasing peptide, with reference to the Proto-Indo-European root ...
National Library of Medicine. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library ... 2001). "Role of the deafness dystonia peptide 1 (DDP1) in import of human Tim23 into the inner membrane of mitochondria". J. ... "The human family of Deafness/Dystonia peptide (DDP) related mitochondrial import proteins". Genomics. 61 (3): 259-67. doi: ... 2000). "The mitochondrial TIM22 preprotein translocase is highly conserved throughout the eukaryotic kingdom". FEBS Lett. 464 ( ...
National Library of Medicine. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library ... Eicosanoid receptor Formyl peptide receptor Lipoxin Resolvin Formyl peptide receptor 1 Formyl peptide receptor 3 GRCh38: ... F peptide, which is derived from gp120 protein of the HIV-1 Bru strain virus, and V3 peptide, which is derived from a linear ... and Formyl peptide receptor 3 (i.e. FPR3). FPR2 and FPR3 are termed formyl peptide receptors base on the similarities of their ...
National Library of Medicine. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library ... SLC27A1 encodes a 71.1 kDa protein that is composed of 646 amino acids; 26 peptides have been observed through mass ... This article incorporates text from the United States National Library of Medicine, which is in the public domain. v t e. ... SLC27A1+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) Solute carrier family GRCh38: ...
"A bipartite bacteriophage T4 SOC and HOC randomized peptide display library: detection and analysis of phage T4 terminase (gp17 ... The T4 DNA packaging motor has been found to load DNA into virus capsids at a rate up to 2000 base pairs per second. The power ... Abedon ST (June 2000). "The murky origin of Snow White and her T-even dwarfs". Genetics. 155 (2): 481-6. PMC 1461100. PMID ... Indication of the prevalence of T4-type sequences in the wild) Abedon S.T. (2000). "The murky origin of Snow White and her T- ...
... lawn format assay to rapidly screen a 442,368 bead-based peptide library". J Pharmacol Toxicol Methods. 42 (4): 189-97. doi: ... Porras, MG; De Loof, A; Breuer, M; Aréchiga, H (2003). "Procambarus clarkii". Peptides. 24 (10): 1581-9. doi:10.1016/j.peptides ... Fujii, R. (2000). "The regulation of motile activity in fish chromatophores". Pigment Cell Res. 13 (5): 300-19. doi:10.1034/j. ... Kelsh, RN; Schmid, B; Eisen, JS (2000). "Genetic analysis of melanophore development in zebrafish embryos". Dev Biol. 225 (2): ...
National Library of Medicine. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library ... Eukaryotic peptide chain release factor GTP-binding subunit ERF3B is an enzyme that in humans is encoded by the GSPT2 gene. ... Overview of all the structural information available in the PDB for UniProt: Q8IYD1 (Human Eukaryotic peptide chain release ... "Assignment of the human peptide chain release factor 3 (GSPT2) to Xp11.23→p11.21 and of the distal marker DXS1039 by radiation ...
National Library of Medicine. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library ... Tuberoinfundibular peptide of 39 residues is a protein that in humans is encoded by the PTH2 gene. TIP39 is related to ... 2003). "Tuberoinfundibular peptide of 39 residues (TIP39): molecular structure and activity for parathyroid hormone 2 receptor ... 2002). "Characterization of the human and mouse genes encoding the tuberoinfundibular peptide of 39 residues, a ligand of the ...
2003). "Interaction of the growth hormone-releasing peptides ghrelin and growth hormone-releasing peptide-6 with the motilin ... Motilin+receptor at the US National Library of Medicine Medical Subject Headings (MeSH) v t e. ... 2001). "Identification of peptide ligand-binding domains within the human motilin receptor using photoaffinity labeling". J. ... Motilin in turn is an intestinal peptide that stimulates contraction of gut smooth muscle. GRCh38: Ensembl release 89: ...
... α-helical antimicrobial peptides". Peptide Science. 55 (1): 4-30. doi:10.1002/1097-0282(2000)55:1. 3.0.CO;2-M. PMID 10931439 - ... doi:10.1111/j.1432-1033.1973.tb02590.x. PMID 4687388 - via Wiley Online Library. Sauviat, Martin-Pierre (January 1, 1990). " ... via Wiley Online Library. Johansson, S.; Gullbo, J.; Lindholm, P.; Ek, B.; Thunberg, E.; Samuelsson, G.; Larsson, R.; Bohlin, L ... Tossi, Alessandro; Sandri, Luca; Giangaspero, Anna (March 11, 2000). "Amphipathic, ...
Afterwards, he conducted postdoctoral research with Professor Fred Blattner on phage display of random peptide libraries and ... He received the Doisy Award in 2000 and the David Perlman Award in 2001. In 2005 he won the NASDAQ-sponsored VCynic Syndicate, ...
In this system, particular proteins are targeted for destruction by the ligation of at least four copies of a small peptide ... Muscular atrophy at the US National Library of Medicine Medical Subject Headings (MeSH). ... 2000). For all these reasons, HMB should be routinely used in muscle-wasting conditions especially in aged people. Wu H, Xia Y ... 2000). For all these reasons, HMB should be routinely used in muscle-wasting conditions especially in aged people. ... 3 g of ...
48: 1-9. doi:10.1016/j.peptides.2013.07.024. PMID 23933301.. *^ Berger M, Neth O, Ilmer M, Garnier A, Salinas-Martín MV, de ... "Fibromyalgia Library. ProHealth.com. Retrieved 2008-11-01.. *Fight Club for Flies video, Science Take, New York Times, February ... Substance P (SP) is an undecapeptide (a peptide composed of a chain of 11 amino acid residues) member of the tachykinin ... Yip J, Chahl LA (Apr 2001). "Localization of NK1 and NK3 receptors in guinea-pig brain". Regulatory Peptides. 98 (1-2): 55-62. ...
Subscription or UK public library membership required.) *^ Newlands, John A. R. (20 August 1864). "On Relations Among the ... Solid state crystal structures have been determined for many complexes of alkali metal ions in small peptides, nucleic acid ... Clark, Jim (2000). "Metallic Bonding". chemguide. Retrieved 23 March 2012.. *^ a b Mason, Philip E. (26 January 2015). "Coulomb ... resistivity of liquid metallic hydrogen at 3000 K is approximately equal to that of liquid rubidium and caesium at 2000 K at ...
Peptides. 31 (8): 1441-8. doi:10.1016/j.peptides.2010.04.021. PMC 2905501 . PMID 20441784. Schein CH, Volk DE, Oezguen N, Paul ... J Invertebr Pathol Additional material "Picornaviridae". Medical Subject Headings (MeSH). National Library of Medicine. ... Peptides. 27 (7): 1676-84. doi:10.1016/j.peptides.2006.01.018. PMC 1629084 . PMID 16540201. Schein CH, Oezguen N, van der Heden ... Schein CH, Oezguen N, Volk DE, Garimella R, Paul A, Braun W (July 2006). "NMR structure of the viral peptide linked to the ...
This article incorporates text from the United States National Library of Medicine, which is in the public domain. ... Wikipedia articles incorporating text from the United States National Library of Medicine ... 2000). "LDL-receptor-related proteins in Wnt signal transduction". Nature. 407 (6803): 530-5. doi:10.1038/35035117. PMID ...
"Florida Atlantic University Libraries. Retrieved 2010-03-31.. *↑ "Leonid Hurwicz (1917-2008)". Jewish Virtual Library. American ... "for the development of radioimmunoassays of peptide hormones"[۶۳] ۱۹۷۸ دنیل ناتانس[۱] United States "for the discovery of ... "for their discoveries concerning the peptide hormone production of the مغز"[۶۳] ... Alan Symons (2000). Nobel laureates, 1901-2000. Polo Publishing. p. 202. ISBN 0952375133. . Tamm was born in Vladivostok, ...
These cells have T-cell receptors and CD4 molecules that, in combination, bind antigenic peptides presented on major ... Leukocytes at the US National Library of Medicine Medical Subject Headings (MeSH) ... Falcone FH, Haas H, Gibbs BF (December 2000). "The human basophil: a new appreciation of its role in immune responses". Blood. ...
"National Center for Biotechnology Information, U.S. National Library of Medicine.. .mw-parser-output cite.citation{font-style: ... Pituitary adenylate cyclase-activating peptide (PACAP). *Pleiotrophin. *Renalase. *Thrombopoietin (see here instead) ... "National Center for Biotechnology Information, U.S. National Library of Medicine.. *^ https://www.sigmaaldrich.com/catalog/ ... Matsuoka Y, Li X, Bennett V (June 2000). "Adducin: structure, function and regulation". Cellular and Molecular Life Sciences. ...
California State Library. ISBN 978-1-58703-133-5. .. *^ "Mold: A Health Hazard (Release #1605-096)". FEMA. November 8, 2005. ... The common house mold, Trichoderma longibrachiatum, produces small toxic peptides containing amino acids not found in common ... Barrett JR (January 2000). "Mycotoxins: of molds and maladies". Environ. Health Perspect. 108 (1): A20-3. doi:10.1289/ehp.108- ... Samson, Robert A., Ellen S. Hoekstra, Jens C. Frisvad (2000). Introduction to food- and airborne fungi (6., rev. ed.). Utrecht ...
Retrieved August 12, 2016 - via Open Library.. *^ a b Pauling L (1976). Vitamin C, the Common Cold, and the Flu. W.H. Freeman ... This increases peptide hormone stability and activity.[99][100]. Pharmacokinetics[edit]. Absorption[edit]. From the U.S. ... United States National Library of Medicine. Archived from the original on May 5, 2009.. ... Eipper BA, Stoffers DA, Mains RE (1992). "The biosynthesis of neuropeptides: peptide alpha-amidation". Annual Review of ...
1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107-13. doi:10.1006/abio. ... 1996). "Inhibition of pRb phosphorylation and cell-cycle progression by a 20-residue peptide derived from p16CDKN2/INK4A". Curr ... Cyclin-Dependent+Kinase+4 at the US National Library of Medicine Medical Subject Headings (MeSH) ... 20 (16): 2000-9. doi:10.1038/sj.onc.1204375. PMID 11360184.. *^ a b Cariou S, Donovan JC, Flanagan WM, Milic A, Bhattacharya N ...
A peptide hormone, gastrin, produced by G cells in the gastric glands, stimulates the production of gastric juice which ... Lower+Gastrointestinal+Tract at the US National Library of Medicine Medical Subject Headings (MeSH) ... The bile is released in response to cholecystokinin (CCK) a peptide hormone released from the duodenum. The production of CCK ( ... Costa, M; Brookes, S J H; Hennig, G W (2000). "Anatomy and physiology of the enteric nervous system". Gut. 47 (90004): iv15- ...
"United States National Library of Medicine - Toxicology Data Network. Hazardous Substances Data Bank. Retrieved 20 September ... In addition to the various beneficial effects for brain function mainly attributed to an upregulation of peptide growth factors ... United States National Library of Medicine - National Center for Biotechnology Information. Retrieved 17 February 2015.. ... United States National Library of Medicine - National Center for Biotechnology Information. Retrieved 28 December 2016.. ...
... in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries". ... Jacobi FK, Broghammer M, Pesch K, Zrenner E, Berger W, Meindl A, Pusch CM (July 2000). "Physical mapping and exclusion of GPR34 ...
... at the US National Library of Medicine Medical Subject Headings (MeSH) ... Woltmann G, McNulty CA, Dewson G, Symon FA, Wardlaw AJ (May 2000). "Interleukin-13 induces PSGL-1/P-selectin-dependent adhesion ...
"National Library of Medicine. Retrieved 12 February 2013.. *^ a b Reece, Jane; Campbell, Neil (2005). Biology (7th ed.). San ... Antinuclear+antibodies at the US National Library of Medicine Medical Subject Headings (MeSH) ... by R. A. (2000). Immunofluorescence in clinical immunology : a primer and atlas. Basel [u.a.]: Birkhäuser. ISBN 978-3764361822 ... doi:10.1043/0003-9985(2000)124,0071:GFCUOT,2.0.CO;2 (inactive 2021-01-17). PMID 10629135.. CS1 maint: DOI inactive as of ...
Once the tRNA is charged, a ribosome can transfer the amino acid from the tRNA onto a growing peptide, according to the genetic ... Amino+Acyl-tRNA+Synthetases at the US National Library of Medicine Medical Subject Headings (MeSH) ... Woese CR, Olsen GJ, Ibba M, Söll D (March 2000). "Aminoacyl-tRNA synthetases, the genetic code, and the evolutionary process". ...
Main article: Peptide synthesis. Short proteins can also be synthesized chemically by a family of methods known as peptide ... Proteins: Biogenesis to Degradation - The Virtual Library of Biochemistry and Cell Biology ... Chemical structure of the peptide bond (bottom) and the three-dimensional structure of a peptide bond between an alanine and an ... "Current Protein & Peptide Science. 9 (4): 338-69. doi:10.2174/138920308785132668. PMC 2904242. PMID 18691124.. ...
2001). "Cell-permeable peptide inhibitors of JNK: novel blockers of beta-cell death". Diabetes. 50 (1): 77-82. doi:10.2337/ ... 1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107-13. doi:10.1006/abio. ... a novel tetratrico peptide repeat-containing adaptor molecule". J. Cell Sci. 116 (Pt 3): 453-61. doi:10.1242/jcs.00243. ISSN ... 2000). "The JIP Group of Mitogen-Activated Protein Kinase Scaffold Proteins". Mol. Cell. Biol. 19 (10): 7245-54. doi:10.1128/ ...
Lanzhou lamb rotavirus vaccine was licensed for use in China in 2000 and is manufactured by the Lanzhou Institute of Biological ...
Hepatocyte+growth+factor at the US National Library of Medicine Medical Subject Headings (MeSH) ... "The procognitive and synaptogenic effects of angiotensin IV-derived peptides are dependent on activation of the hepatocyte ... Sergeant N, Lyon M, Rudland PS, Fernig DG, Delehedde M (June 2000). "Stimulation of DNA synthesis and cell proliferation of ... Gallagher, J.T., Lyon, M. (2000). "Molecular structure of Heparan Sulfate and interactions with growth factors and morphogens ...
"National Center for Biotechnology Information, U.S. National Library of Medicine.. *^ Funk CD, Hoshiko S, Matsumoto T, Rdmark O ... Certain of these peptide-leukotrienes have been shown to promote the growth of cultured human breast cancer and chronic ... United States National Library of Medicine.. *^ Clinical trial number NCT00404313 for "The Effect of MK0633 in Patients With ... "National Center for Biotechnology Information, U.S. National Library of Medicine.. .mw-parser-output cite.citation{font-style: ...
"The Wellcome Library. Retrieved 2014-03-15.. *^ McKie, Robin (17 September 2006). "Genius was in his DNA". The Guardian. London ... the importance of the structural rigidity that double bonds confer on molecular structures which is relevant both to peptide ... "The Wellcome Library. Retrieved 2014-03-15.. *^ Crick, Francis. "Letter to the Editor, Varsity, the University of Cambridge ... "Special Collections, The Valley Library, Oregon State University.. *^ Chapter 3 in The Eighth Day of Creation by Horace Judson. ...
The Human ATP-Binding Cassette (ABC) Transporter Superfamily. Bethesda (MD):National Library of Medicine (US), NCBI; 2002 ... SkfA Peptide Exporter (SkfA-E). *CydDC Cysteine Exporter (CydDC-E). Jegyzetek[szerkesztés]. *↑ Jones, P. M.; George, A. M.; ... a b Saier MH Jr.; Microbiol Mol Biol Rev., 2000, 64(2), 354-411 TCDB ...
... s at the US National Library of Medicine Medical Subject Headings (MeSH) ... The molecules secreted vary in size from the small Escherichia coli peptide colicin V, (10 kDa) to the Pseudomonas fluorescens ... allowing the rest of the peptide (the passenger domain) to reach the outside of the cell. Often, autotransporters are cleaved, ... which requires the presence of an N-terminal signal peptide on the secreted protein. Others are translocated across the ...
Ziconotide, a peptide compound derived from the omega-conotoxin, is a selective N-type calcium channel blocker that has potent ... Calcium+Channel+Blockers at the US National Library of Medicine Medical Subject Headings (MeSH) ... Norman M Kaplan, MD; Burton D Rose, MD (Apr 3, 2000). "Major side effects and safety of calcium channel blockers". Chinese ...
response to peptide hormone. • response to organic substance. • positive regulation of axonogenesis. • MAPK cascade. • ... end-enriched cDNA library". Gene. 200 (1-2): 149-56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.. ... Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin AN, Evgrafov OV (July 2000). "A new ...
US National Library of Medicine. The Albert Szent-Györgyi Papers.NIH Profiles in Science ... Gábor Tóth: From vitamins to peptides - Research topics in Szent-Györgyi's departments. In: The intellectual heritage of Albert ... The Albert Szent-Gyorgyi Papers - Profiles in Science, National Library of Medicine ... Kyle, R. A.; Shampo, M. A. (2000). "Albert Szent-Györgyi--Nobel laureate". Mayo Clinic Proceedings. 75 (7): 722. doi:10.4065/ ...
"United States National Library of Medicine, National Institutes of Health. 2009.. *^ a b c d e Young VR, Pellett PL (May 1994 ... Current milk-testing equipment measures peptide bonds, a direct measure of true protein."[15] Measuring peptide bonds in grains ... most proteins are already reduced to single amino acid or peptides of several amino acids. Most peptides longer than four amino ... Proteins are polymer chains made of amino acids linked together by peptide bonds. During human digestion, proteins are broken ...
This article incorporates text from the United States National Library of Medicine, which is in the public domain. ... Wikipedia articles incorporating text from the United States National Library of Medicine ... Liu J, Akahoshi T, Jiang S, Namai R, Kitasato H, Endo H, Kameya T, Kondo H (August 2000). "Induction of neutrophil death ...
An all D-amino acid opioid peptide with central analgesic activity from a combinatorial library. Science. 1994; 266: 2019-22. * ... Tight-binding streptavidin ligands from a cyclic peptide library. Anal Chem. 1997; 69: 4515-18. *CrossRef, ... How small peptides block and reverse serpin polymerization. J Mol Biol. 2004; 342: 931-41. *CrossRef, ... A high-throughput screen for compounds that inhibit aggregation of the Alzheimers peptide. ACS Chem Biol. 2006; 1: 461-9. * ...
Now, the mechanisms employed by entomopathogenic bacteria for the biosynthesis of a large family of bioactive peptides have ... Here we report mechanisms leading to the biosynthesis of the chemically diverse rhabdopeptide/xenortide peptides (RXPs). They ... the reactions responsible for generating such diverse chemical libraries are often not clear. ... little is known about the mechanisms generating such chemically diverse libraries. ...
B-type natriuretic peptide and adiponectin releases in rat model of myocardial damage induced by isoproterenol administration.( ... of Physiological aspects Heart diseases Isoproterenol Complications and side effects Myocardial diseases Natriuretic peptides ... Chicago style: The Free Library. S.v. B-type natriuretic peptide and adiponectin releases in rat model of myocardial damage ... 2000-2004 2005-2009 2010- A B C D E F G H I J K L M N O P Q R S T U V W X Y Z A B C D E F G H I J K L M N O P Q R S T U V W X Y ...
... general Peptides Health aspects Identification and classification Research Usage Virus diseases Drug therapy Prevention Risk ... Antiviral activity and mode of action of a peptide isolated from Sorghum bicolor.(Report) by Phytomedicine: International ... APA style: Antiviral activity and mode of action of a peptide isolated from Sorghum bicolor.. (n.d.) >The Free Library. (2014 ... 2000-2004 2005-2009 2010- A B C D E F G H I J K L M N O P Q R S T U V W X Y Z A B C D E F G H I J K L M N O P Q R S T U V W X Y ...
The peptide is followed by a short spacer (Gly-Gly-Gly-Ser) and then the wild-type pIII sequence. The library consists of ... The displayed peptide is expressed at the N-terminus of pIII, i.e., the first residue of the mature protein is the first ... C7C Phage Display Peptide Library is based on a combinatorial library of random peptides fused to a minor coat protein (pIII) ... Ph.D.™-C7C Phage Display Peptide Library Kit The Ph.D.™-C7C Phage Display Peptide Library Kit contains a tube of the Ph.D.-C7C ...
2000 Jan 18;18(13):1253-63. Comparative Study; Research Support, Non-U.S. Govt ... A direct approach to identification of peptide mimics of antigen is through the use of peptide display libraries. We have ... immunogenic peptide mimic of the capsular polysaccharide of Neisseria meningitidis serogroup A using a peptide display library. ... Immunization with motif 1 peptide complexed to proteosomes resulted in an anti-MAPS antibody response. Priming with the peptide ...
Phage display is a selection technique in which a library of peptide or protein variants is expressed as a genetic fusion to a ... Applications of the Ph.D. Phage Display Peptide Libraries. Phage display is a selection technique in which a library of peptide ... Combinatorial peptide libraries displayed on phage have allowed rapid screening of billions of different peptides against a ... Figure 1: Panning with a pentavalent peptide library displayed on pIII.. Anti-viral/microbial peptides. In a search for ...
Here, specific chicken bone marrow DC-binding peptides were selected using a phage display peptide library and confirmed ... The peptide candidate SPHLHTSSPWER, named SP, was fused to the infectious bursal disease virus (IBDV) structural protein and ... The peptide candidate SPHLHTSSPWER, named SP, was fused to the infectious bursal disease virus (IBDV) structural protein and ... by VP2-SP-stimulated DCs was significantly higher than that by DCs treated with the VP2-control peptide at 4 h (p < 0.001). In ...
Antigen binding peptides (abtides) from peptide libraries. US5935818 *. Feb 24, 1995. Aug 10, 1999. Sloan-Kettering Institute ... Antigen binding peptides (abtides) from peptide libraries. US6017903. Jun 27, 1997. Jan 25, 2000. Guilford Pharmaceuticals Inc. ... Random peptide library. US5788963 *. Jul 31, 1995. Aug 4, 1998. Pacific Northwest Cancer Foundation. Isolation and/or ... For instance, if the antigen is a protein, an antigenic determinant may be one of the many peptide sequences [generally 6-7 ...
... we screened a phage display peptide library (PDL) on the surface of iRBCs. After the sixth panning of the PDL, eight phage ... When P1 peptide was conjugated with a peptide having moderate hemolytic activity, the peptide conjugate inhibited the growth of ... and specific binding of P1 to the surface of iRBCs was confirmed using phage expressing P1 peptides and synthetic P1 peptide. ... Our results demonstrate that the P1 peptide may be a lead compound for the development of anti-malarial agents targeting the ...
A phage display library was used to identify peptide sequences that target bone. The objective was to investigate the effect of ... Cwirla, S. E., Peters, E. A., Barrett, R. W., Dower, W. J. Peptides on phage: a vast library of peptides for identifying ... A phage display library was used to identify peptide sequences that target bone. The objective was to investigate the effect of ... The Ph.D-12 Phage display peptide library kit (New England Biolabs, Beverly, MA) was used for in vivo biopanning, 10 x 1010 pfu ...
Utilization of oriented peptide libraries to identify substrate motifs selected by ATM. J Biol Chem 275, 22719-27 (2000). ... Final DNA libraries were validated and processed with Illumina cBot for cluster generation on the flowcell, following the ... Library preparation and sequencing. DNA recovered from the ChIP procedure was quantified using the Qubit 2.0 Fluorometer ( ...
Prevention of passively transferred experimental autoimmune myasthenia gravis by a phage library-derived cyclic peptide ... Drug target validation: Lethal infection blocked by inducible peptide Jianshi Tao, Philip Wendler, Gene Connelly, Audrey Lim, ... Patterned library analysis: A method for the quantitative assessment of hypotheses concerning the determinants of protein ... PNAS January 18, 2000 97 (2) 526-528; https://doi.org/10.1073/pnas.97.2.526 ...
Cell-specific peptides can be useful as drug delivery vehicles, diagnostic agents, affinity reagents for cell purification, ... Peptides that recognize specific cell types promise to be valuable tools both in research and clinical applications. ... New Approaches for Cell-Specific Targeting: Identification of Cell-Selective Peptides From Combinatorial Libraries Curr Opin ... Peptides that recognize specific cell types promise to be valuable tools both in research and clinical applications. Cell- ...
Peptide and protein library screening defines optimal substrate motifs for AKT/PKB. J. Biol. Chem. 275, 36108-36115 (2000).. ...
SH2 matrices from oriented peptide array libraries. The starting oriented peptide library contained the sequence KX3-pY-X5D, ... Peptide and protein library screening defines optimal substrate motifs for AKT/PKB. J. Biol. Chem. 275, 36108-16115 (2000).. ... An oriented peptide array library (OPAL) strategy to study protein-protein interactions. J. Biol. Chem. 279, 8802-8807 (2004). ... Figure S6: Kinase matrices from Positional Scanning Peptide Libraries (PSPL).. Figure S7: Sequence logos for kinases and pS/pT- ...
Libraries of peptides and proteins displayed on filamentous phage. Selvaraj et al. 1983. Suicide plasmid vehicles for insertion ... C40B-COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES * C40B40/00-Libraries per se, e.g. arrays ... Lu et al., Bio/Technology 13: 366 372, 1995, Expression of Thioredoxin Random Peptide Libraries on the Escherichia coli Cell ... Lu et al., Bio/Technology 13: 366-372, 1995, Expression of Thioredoxin Random Peptide Libraries on the Escherichia coli Cell ...
Construction of such a peptide-fused phage library possessing non-natural core structures will be useful for future drug ... conjugated with surrounding randomized peptides. By using the hybrid library, streptavidin-specific binders are isolated ... The detailed structure of the modified peptide on phage is identified with tandem mass spectrometry. ... site-specific conjugation of several haloacetamide derivatives into designated cysteines on bacteriophage T7-displayed peptides ...
By their targeting of short, linear motif type of interactions, peptide aptamers have joined nucleic acid aptamers for use in ... We propose that peptide aptamers can provide a very useful and new alternative for interfering with protein-protein ... Geyer, C.R.; Brent, R. Selection of genetic agents from random peptide aptamer expression libraries. Methods Enzymol 2000, 328 ... FKBP12-(peptide)-FRB-GST. fusion of three domains. screen for kinase inhibitors. [27]. ligand-regulated peptide aptamers (LiRPs ...
Determination of substrate motifs for human Chk1 and hCds1/Chk2 by the oriented peptide library approach. J Biol Chem. 2002;277 ... Anti-SYK-23, an antibody specific for SYK(L), was generated by immunizing rabbits with the coupled peptide 283 ... was generated by immunizing rabbits with the coupled peptide SRIKS295YSFPKPGHRKC. ... FEBS Lett. 2000;466(1):91-95.. View this article via: PubMed CrossRef Google Scholar ...
Utilization of oriented peptide libraries to identify substrate motifs selected by ATM. J Biol Chem 275:22719-22727, doi: ... Peptides were desalted using a peptide microtrap (Bruker-Michrom). Desalting was accomplished using 400 μl 95:5 solvents A:B ( ... Desalted peptides were eluted from the microtrap with 200 μl 70:30 solvents A:B at 100 μl/min and dried in a speed vac. The ... 2004) Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry. Proc Natl Acad Sci U S A 101: ...
Peptide Spectral Library. Samples from early stage, mid stage and late stage were acquired in 2 biological replicates and 2 ... Number of peptides per protein 6, number of transitions per peptide 10, Peptide confidence 95%, FDR threshold 1%, XIC ... 2015) Development of diagnostic fragment ion library for glycated peptides of human serum albumin: targeted quantification in ... The libraries were then subjected to PCR enrichment (15 cycles) and again validated using Bioanalyzer. Libraries were then ...
... peptide library synthesis and peptide reagents for immunology, drug discovery, bioassay development, epitope mapping, ... Home , Custom Peptides , Peptide Libraries , Catalogue , Resources , About Us , FAQs , Contact Us , Terms & Conditions , Web ... online peptide superstore provides custom peptide synthesis, ... Peptide Libraries * PepSets - Peptide Libraries * Peptide ...
Targeting Plasmodium ligands on mosquito salivary glands and midgut with a phage display peptide library. Proc Natl Acad Sci ... 2000). In another study using Pvs25 and an adenovirus as the adjuvant, BALB/c mice were immunized four times by different ... 2000). Other proteins, including a similar von Willebrand factor type A domain (WARP), are present as soluble products in the ... 2000, Stowers & Carter 2001). These antigens have strong potential for boosting the immune responses elicited naturally during ...
Combinatorial peptide libraries reveal the ligand-binding mechanism of the oligopeptide receptor OppA of Lactococcus lactis. ... 2005) Specificity and selectivity determinants of peptide transport in Lactococcus lactis and other microorganisms. Mol ... can use proteins only by degrading them at the cell surface using proteases and incorporating the resulting peptides (25). A 35 ...
Utilization of oriented peptide libraries to identify substrate motifs selected by ATM. J. Biol. Chem. 275, 22719-22727. ... Phosphopeptides were isolated from 2 mg of total peptide mass with TiO2 as described previously (38). Briefly, dried peptides ... The peptides were eluted with a 0.3 m NH4OH solution. The pH of the eluates was adjusted to be below 2.5 with 5% TFA solution ... Peptides were added to the same amount of equilibrated TiO2 (5-μm bead size, GL Sciences, Tokyo, Japan) in a blocked Mobicol ...
Identification of T Cell Ligands in a Library of Peptides Covalently Attached to HLA-DR4 Eric Boen, Angie R. Crownover, Mary ... The MHC Class II Molecule I-Ag7 Exists in Alternate Conformations That Are Peptide Dependent Lynne S. Arneson, Mary Peterson ... J Immunol August 15, 2000, 165 (4) 1725-1728; DOI: https://doi.org/10.4049/jimmunol.165.4.1725 ... J Immunol August 15, 2000, 165 (4) 1729-1732; DOI: https://doi.org/10.4049/jimmunol.165.4.1729 ...
Substrate specificity of human collagenase 3 assessed using a phage-displayed peptide library. J Biol Chem 275(40), 31422-7. ... Identification of substrate sequences for membrane type-1 matrix metalloproteinase using bacteriophage peptide display library ... 2003) Identification of peptide substrates for human MMP-11 (stromelysin-3) using phage display. J Biol Chem 278(30), 27820-7. ... 2. Berger A, Schechter I. (1970) Mapping the active site of papain with the aid of peptide substrates and inhibitors. Philos ...
Library selection.Vectors containing CLPIT VSV-G-His6 library genomes pseudotyped with WT VSV-G were used to infect 293-gag-pol ... The use of a transposon-based method to create a random peptide insertion library did not necessitate the structural knowledge ... Construction of the VSV-G-His6 library.We first constructed a library of vsv-g-Kanr insertion mutants by using MuA transposase ... VSV-G-His6 library design. (A) Structure of the pCLPIT VSV-G-His6 vector, which expresses the VSV-G-His6 library from a ...
AND PPARGAMMA LIGAND BINDING DOMAINS RESPECTIVELY BOUND WITH 9-CIS RETINOIC ACID AND GI262570 AND CO-ACTIVATOR PEPTIDES. ... SWISS-MODEL Template Library (SMTL) entry for 1fm9.1. THE 2.1 ANGSTROM RESOLUTION CRYSTAL STRUCTURE OF THE HETERODIMER OF THE ... Peptides. RETINOIC ACID RECEPTOR RXR-ALPHA: A. PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA: B. STEROID RECEPTOR ... AND PPARGAMMA LIGAND BINDING DOMAINS RESPECTIVELY BOUND WITH 9-CIS RETINOIC ACID AND GI262570 AND CO-ACTIVATOR PEPTIDES.. ...
  • Figure 4: Dependence of RXP chain length on protein stoichiometry of proteins involved in peptide elongation (Kj12B) and peptide termination (Kj12C) as shown by Western blot, HPLC/MS analysis and in vitro experiments using purified Kj12B and Kj12C. (nature.com)
  • By their targeting of short, linear motif type of interactions, peptide aptamers have joined nucleic acid aptamers for use in signaling studies because of their ease of production, their stability, their high specificity and affinity for individual target proteins, and their use in high-throughput screening protocols. (mdpi.com)
  • Nucleic acid aptamers are short, single-stranded RNAs and DNAs that represent high-affinity and highly selective ligands for different targets, ranging from large proteins to peptides, nucleotides, drugs and small compounds, and even metal ions. (mdpi.com)
  • Phage-display technology has been improved to enable the identification of peptides that bind to carbohydrate-binding proteins, such as lectins and antibodies, from a large repertoire of peptide sequences. (hindawi.com)
  • Macbeath, G. and Schreiber, S.L. (2000) Printing proteins as microarrays for high-throughput function determination. (springer.com)
  • The process results in translated peptides or proteins that are associated with their mRNA progenitor via a puromycin linkage. (wikipedia.org)
  • This work thus demonstrates the feasibility of displaying complex Fab libraries as pIX-fusion proteins for antibody discovery and lays the foundations for studies on the structure-function relationship of antibodies. (freepatentsonline.com)
  • Peptide separations are of paramount importance, whether studying proteins by bottom-up proteomics 1 or thoroughly characterizing biopharmaceuticals using peptide mapping. (waters.com)
  • Glutathione S -transferase pull-down assays with 14-3-3 fusion proteins revealed that all seven 14-3-3 isoforms (β, γ, ζ, ε, τ, η, and ς) could bind tuberin, and this interaction was abrogated by competition with phosphorylated but not unphosphorylated Ser 939 tuberin peptide. (aacrjournals.org)
  • Metagenomic libraries derived from microbial community genomes can be screened for heterologous phenotypic traits that include enzymes and other proteins that are essential to the functioning of the ecosystem ( 27 ). (asm.org)
  • By expressing metal binding proteins and peptides in bacteria and plants the metal tolerance or accumulation of the organism can be increased. (lu.se)
  • Isogenica specialises in the discovery and optimisation of therapeutic and diagnostic peptides, proteins and antibodies using both conventional technologies and proprietary capabilities. (businesswire.com)
  • Phage display is a laboratory technique for the study of protein-protein, protein-peptide, and protein-DNA interactions that uses bacteriophages (viruses that infect bacteria) to connect proteins with the genetic information that encodes them. (wikipedia.org)
  • These displaying phages can then be screened against other proteins, peptides or DNA sequences, in order to detect interaction between the displayed protein and those other molecules. (wikipedia.org)
  • In this way, large libraries of proteins can be screened and amplified in a process called in vitro selection, which is analogous to natural selection. (wikipedia.org)
  • The library consists of approximately 1 x 10 9 electroporated (i.e., unique) sequences. (neb.com)
  • The library consists of approximately 10 9 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage. (neb.com)
  • A phage display library was used to identify peptide sequences that target bone. (jove.com)
  • Briefly, a large library of high-complexity oligonucleotide sequences, comprised of a randomized region that is flanked by constant regions carrying annealing sites for primers in PCR-based amplification, is incubated with the target, e.g., a purified protein. (mdpi.com)
  • 6. Ohkubo S, Miyadera K, Sugimoto Y, Matsuo K, Wierzba K, Yamada Y. (1999) Identification of substrate sequences for membrane type-1 matrix metalloproteinase using bacteriophage peptide display library. (springer.com)
  • From just a microscopic sample of bone, their analysis revealed the collagen peptide sequences in the bone that mark out one species from another. (ox.ac.uk)
  • The sequences of collagen peptides in bone differ in tiny ways between different animal species. (ox.ac.uk)
  • They then compared the sequences obtained against a reference library of peptides from known animal species. (ox.ac.uk)
  • Xenome has leveraged the identification of venom peptide sequences into high quality synthesised peptides arrayed into a library suitable for high throughput screening. (biospace.com)
  • Aldehyde-modified peptide sequences in apolipoprotein B-100 (apoB-100) are major targets for these immune responses. (ahajournals.org)
  • Methods and Results- Human IgG1 antibodies against 2 malondialdehyde (MDA)-modified apoB-100 peptide sequences were produced through screening of a single-chain antibody-fragment library and subsequent cloning into a pcDNA3 vector. (ahajournals.org)
  • Thus, passive immunization against MDA-modified apoB-100 peptide sequences may represent a novel therapeutic approach for prevention and treatment of cardiovascular disease. (ahajournals.org)
  • Several undergraduates have participated in projects that have involved (i) screening 7-mer and 12-mer peptide libraries to identify putative zinc-binding peptides, (ii) developing a phage-ELISA assay to quantify the zinc-binding of individual peptides sequences, and (iii) characterizing the structure of Zn 2+ -peptide complexes using NMR. (stonehill.edu)
  • We compared all 18 peptide sequences (10 most potent, 7 peptides with sequence similarity to hantavirus glycoproteins, and 1 peptide that was in the group that displayed the greatest potency and had significant sequence similarity) for their abilities to inhibit SNV, Hantaan virus (HTNV), and Prospect Hill virus (PHV) infection. (asm.org)
  • We identified four novel substrate sequences for HER2/Neu from the two peptide libraries. (cshl.edu)
  • We synthesized these peptides as individual sequences and measured steady-state kinetic properties for phosphorylation by HER2/Neu. (cshl.edu)
  • Phage display was first described by George P. Smith in 1985, when he demonstrated the display of peptides on filamentous phage (long, thin viruses that infect bacteria) by fusing the virus's capsid protein to one peptide out of a collection of peptide sequences. (wikipedia.org)
  • Applications of phage display technology include determination of interaction partners of a protein (which would be used as the immobilised phage "bait" with a DNA library consisting of all coding sequences of a cell, tissue or organism) so that the function or the mechanism of the function of that protein may be determined. (wikipedia.org)
  • The Ph.D.™-C7C Phage Display Peptide Library Kit contains a tube of the Ph.D.-C7C Phage Library, protein reagents for a control reaction and sequencing primer stocks. (neb.com)
  • The Ph.D.-C7C library is a combinatorial library of random peptides with a disulfide constrained loop, fused to a minor coat protein (pIII) of M13 phage. (neb.com)
  • The displayed peptide is expressed at the N-terminus of pIII, i.e., the first residue of the mature protein is the first randomized position. (neb.com)
  • Alternatively, the meningococcal polysaccharide can be rendered into a T dependent antigen through the use of peptides which mimic the capsular polysaccharide complexed or conjugated to potent protein carrier molecules. (nih.gov)
  • Phage display is a selection technique in which a library of peptide or protein variants is expressed as a genetic fusion to a bacteriophage coat protein. (neb.com)
  • Over the last ten years, the Ph.D. libraries from New England Biolabs have become the dominant tools in this field, with hundreds of publications describing applications including epitope mapping (4-10), mapping protein-protein contacts (11-16) and identification of peptide mimics of non-peptide ligands (17,18). (neb.com)
  • Blocking protein-protein interaction with peptides discovered by phage display will have broad clinical applications for treatment of metastasizing tumors. (neb.com)
  • The peptide candidate SPHLHTSSPWER, named SP, was fused to the infectious bursal disease virus (IBDV) structural protein and protective antigen VP2. (frontiersin.org)
  • We have achieved site-specific conjugation of several haloacetamide derivatives into designated cysteines on bacteriophage T7-displayed peptides, which are fused to T7 capsid protein gp10. (mdpi.com)
  • We propose that peptide aptamers can provide a very useful and new alternative for interfering with protein-protein interactions in intracellular signal transduction cascades, including those emanating from activated receptors for growth factors. (mdpi.com)
  • We have developed a transposon-based approach that randomly incorporates designed polypeptides throughout a protein to generate saturated insertion libraries and a subsequent high-throughput selection process in mammalian cells that enables the identification of optimal insertion sites for a novel designed functionality. (asm.org)
  • This method was applied to VSV-G in order to construct a comprehensive library of mutants whose combined members have a His 6 tag inserted at likely every site in the original protein sequence. (asm.org)
  • Furthermore, this approach of library generation and selection can readily be extended to other challenges in protein engineering. (asm.org)
  • Modifications to the extracellular domain of VSV-G, however, have been hindered by a lack of structural knowledge of the protein and limited identification of permissible insertion sites ( 14 , 28 , 37 ), making it an excellent candidate for applying a novel library mutagenesis method to improve its functionality. (asm.org)
  • Library generation and selection approaches have been broadly successful for engineering or enhancing features of a target protein in the absence of detailed structural knowledge ( 49 ). (asm.org)
  • Fusing or inserting a peptide or domain into a target protein may introduce novel capabilities, but identifying optimal fusion locations in the absence of structural information is challenging. (asm.org)
  • Therefore, novel protein libraries with polypeptides inserted at random locations may offer a high-throughput means of enhancing protein function. (asm.org)
  • Such mimetic peptides are available as peptide inhibitors of carbohydrate-protein interactions and peptide mimotopes that are conjugated with adjuvant for vaccination. (hindawi.com)
  • Use of peptide retention time prediction for protein identification by off-line reversed-phase HPLC-MALDI MS/MS. (nih.gov)
  • The peptide array has come into focus as an emerging screening platform for large-scale protein detection and activity studies. (springer.com)
  • Li, M. (2000) Applications of display technology in protein analysis. (springer.com)
  • MacBeath, G., Koehler, A.N., and Schreiber, S.L. (1999) Printing small molecules as microar-rays and detecting protein-ligand interactions en masse. (springer.com)
  • I propose that CT, CGRP, adrenomedullin, and amylin belong to a family of G-protein-coupled receptors (an "insulin superfamily" of peptides) and therefore share some of the characteristics of insulin, such as growth factor-like effects, and possible interaction at insulin receptor sites as an antagonist. (begellhouse.com)
  • mRNA display is a display technique used for in vitro protein, and/or peptide evolution to create molecules that can bind to a desired target. (wikipedia.org)
  • A DNA library for any protein or small peptide of interest can be synthesized by solid-phase synthesis followed by PCR amplification. (wikipedia.org)
  • A variety of high-throughput approaches, including SEREX, 1 phage display, 2 , 3 and peptide/protein arrays, 4 , 5 have been developed to identify disease specific antibodies to protein and peptide antigens from the repertoire of antibodies present in serum. (pubmedcentralcanada.ca)
  • Described and claimed herein are combinatorial synthetic Fab libraries displayed on a phage pIX protein. (freepatentsonline.com)
  • Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose‐binding protein. (currentprotocols.com)
  • He is the Editor-in-Chief of two Bentham journals, Protein and Peptide Letters, for research reports and mini-reviews, and Current Protein and Peptide Science, which publishes exclusively review papers. (benthamscience.com)
  • Current protein and Peptide Science was introduced in 2000 with the assistance of Dr. Ben M. Dunn, who is the current Editor-in-Chief serving since 2000. (benthamscience.com)
  • Walter G, Büssow K, Cahill D, Lueking A, Lehrach H (2000) Protein arrays for gene expression and molecular interaction screening . (springer.com)
  • Büssow K, Cahill D, Nietfeld W, Bancroft D, Scherzinger E, Lehrach H, Walter G (1998) A method for global protein expression and antibody screening on highdensity filters of an arrayed cDNA library . (springer.com)
  • Using a protein domain microarray, a tuberin peptide containing Ser 939 demonstrated phospho-specific binding to 14-3-3. (aacrjournals.org)
  • 2,3 The LDL protein apolipoprotein B-100 (apoB-100) is degraded, and aldehydes bind to free amino groups on the peptide fragments. (ahajournals.org)
  • From 1998-2000, Dr. Hall carried out post-doctoral research at New England Biolabs in Beverly, MA creating an artificial bifunctional intein capable of both protein splicing and homing endonuclease activity. (stonehill.edu)
  • Employing yeast two-hybrid screening of a human embryonic kidney cDNA library with the carboxyl-terminal cytoplasmic domain of the AT1 receptor as a bait, we have isolated EP24.15 (EC 3.4.24.15, thimet oligopeptidase) as a potentially interacting protein. (embl.de)
  • Soluble bivalent HLA-DR2.myelin basic protein (MBP) peptide complexes were expressed by replacing the F(ab) arms of an IgG2a antibody with HLA-DR2.MBP peptide complexes. (semanticscholar.org)
  • The cadmium binding peptide fused to the N-terminal of green fluorescent protein also endowed tobacco plants with an increased cadmium tolerance. (lu.se)
  • Isogenica is currently commercialising the Colibra™ technology for ratio controlled protein and antibody libraries based on a partly automated process. (businesswire.com)
  • Founded in 2000, Isogenica has developed a unique capability in the field of protein engineering. (businesswire.com)
  • The present disclosure relates to nutritional compositions including a protein equivalent source that includes a peptide component comprising selected peptides. (patents.com)
  • In the case of M13 filamentous phage display, the DNA encoding the protein or peptide of interest is ligated into the pIII or pVIII gene, encoding either the minor or major coat protein, respectively. (wikipedia.org)
  • LILRA3+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) Overview of all the structural information available in the PDB for UniProt: Q8N6C8 (Leukocyte immunoglobulin-like receptor subfamily A member 3) at the PDBe-KB. (wikipedia.org)
  • Sieber, S. A. & Marahiel, M. A. Molecular mechanisms underlying nonribosomal peptide synthesis: approaches to new antibiotics. (nature.com)
  • Geysen, H.M., Melven, R.H., and Barteling, S.J. (1984) Use of peptide synthesis to probe viral antigen54s for epitopes to a resolution of a single amino acid. (springer.com)
  • Houghten, R.A. (1984) General method for the rapid solid-phase synthesis of large numbers of peptides: Specificity of antigenantibody interaction at the level of individual amino acids. (springer.com)
  • The synthesis of an mRNA display library starts from the synthesis of a DNA library. (wikipedia.org)
  • Versatile Building Block for the Synthesis of Phosphine-Containing Peptides: The Sulfide of Diphenylphosphinoserine," J. Am. Chem. (patentgenius.com)
  • Synthesis of Phosphine-Rhodium Complexes Attached to a Standard peptide Synthesis Resin," Organometallics, vol. 15, pp. 4678-4680, (1996). (patentgenius.com)
  • For several reasons, the initial work in combinatorial library construction focused on peptide synthesis. (google.es)
  • The scope of combinatorial chemistry libraries has recently been expanded beyond peptide synthesis. (google.es)
  • on January 4, 2018 10:11 PM But not shop peptide synthesis and covers broad assignments. (mistletunes.com)
  • on January 1, 2018 10:53 PM One of the references, from an one-armed spectrometers shop peptide synthesis and, is to be m with straightforward functions of item for pleasure, end, and paper to understand a composite and affordable resonance. (mistletunes.com)
  • This support from the TSB follows the successful development by Isogenica of its Colibra™ technology for the commercial synthesis of ratio controlled gene libraries using a patent protected methodology discovered by Dr Anna Hine of Aston University which was also supported by TSB funding. (businesswire.com)
  • We have previously developed an anti-idiotypic monoclonal antibody (mAb) based peptide mimic of meningococcal group C polysaccharide (MCPS). (nih.gov)
  • Immunization with motif 1 peptide complexed to proteosomes resulted in an anti-MAPS antibody response. (nih.gov)
  • a fluorogenic biosensor was developed by conjugating a fluorophore with a designated Cys at the antigen-binding site of antibody library on the phage [ 4 ]. (mdpi.com)
  • de Wildt RM, Mundy CR, Gorick BD, Tomlinson IM (2000) Antibody arrays for high-throughput screening of antibody-antigen interactions . (springer.com)
  • By metabolomics we aim to find additional markers from diabetic urine, and will identify antibodies recognizing these metabolites/peptides from an antibody phage display-library. (europa.eu)
  • Potential of Peptides Selected from Random Phage-Displayed Libraries to Mimic Conformational Epitopes: A Study on Scorpion Toxin Cn2 and the Neutralizing Monoclonal Antibody BCF2 Combinatorial Chemistry & High Throughput Screening, 6, 119-132. (unam.mx)
  • Isogenica's CIS display technology is an in vitro display technology that allows the rapid generation of polypeptide and antibody libraries from which it is possible to select lead molecules with high affinity and specificity for most targets. (businesswire.com)
  • Identification of peptide motifs recognized by a human IgG autoanti-IgE antibody using a phage display library. (dmu.ac.uk)
  • 2000) Prediction of the interacting surfaces in a trimolecular complex formed between the major dust mite allergen Der p1, a mouse monoclonal anti-Der p 1 antibody and its anti-idiotype. (dmu.ac.uk)
  • We have utilized a phage library and a mAb with specificity for meningococcal group A polysaccharide (MAPS) to screen for a peptide mimic of MAPS. (nih.gov)
  • During her post-doc, she was also an adjunct professor at Massasoit Community College, teaching general chemistry from 1999-2000. (stonehill.edu)
  • The xdiscover™ library is a proprietary collection of more than 2000 primarily venom-derived peptides and is the result of a unique genomics capability that allows for the highly efficient discovery of novel peptides from cone snail venom tissue. (biospace.com)
  • In all, we have identified novel peptides that inhibit entry by SNV and HTNV via β 3 integrins and that can be used as lead compounds for further structural optimization and consequent enhancement of activity. (asm.org)
  • 2000) Substrate specificity of human collagenase 3 assessed using a phage-displayed peptide library. (springer.com)
  • Jiang Z, Vasil AI, Gera L, Vasil ML, Hodges RS (2011) Rational design of alpha-helical antimicrobial peptides to target Gram-negative pathogens, Acinetobacter baumannii and Pseudomonas aeruginosa: utilization of charge, 'specificity determinants,' total hydrophobicity, hydrophobe type and location as design parameters to improve the therapeutic ratio. (oalib.com)
  • explained the decreased peptide specificity of the alloreactive T cell clone 2.102 with certain allelic α-helical residues of the foreign MHC, which supposedly supply more binding energy to the TCR than the respective self-MHC molecule ( 19 ). (rupress.org)
  • Overall, the autoMACS® platform quickly harvested higher affinity peptide candidates with demonstrated specificity to the PA target. (omicsonline.org)
  • For instance, substitution of Pro3' in the Smac-based peptide (AVPIAQKSE) with (2S,3S)-3-methylpyrrolidine-2-carboxylic acid [(3S)-methyl-proline] results in a peptide with 7-fold greater affinity for ML-IAP-BIR and about 100-fold specificity for ML-IAP-BIR relative to XIAP-BIR3. (rcsb.org)
  • Cell-specific peptides can be useful as drug delivery vehicles, diagnostic agents, affinity reagents for cell purification, gene therapy delivery agents, and research tools to probe the nature of a cell's surface. (nih.gov)
  • Selecting the library via iterative retroviral infections of mammalian cells led to the identification of several VSV-G-His 6 variants that were able to package high-titer viral vectors and could be purified by Ni-nitrilotriacetic acid affinity chromatography. (asm.org)
  • The result is a nucleotide sequence that encodes a peptide with high affinity for the molecule of interest. (wikipedia.org)
  • X-ray crystal structures of ML-IAP-BIR in complex with Smac- and phage-derived peptides, together with peptide structure-activity-relationship data, indicate that the peptides can be modified to provide increased binding affinity and selectivity for ML-IAP-BIR relative to XIAP-BIR3. (rcsb.org)
  • This displayed the different peptides on the outer surfaces of the collection of viral clones, where the screening step of the process isolated the peptides with the highest binding affinity. (wikipedia.org)
  • This ultrasound-based method enables single-step selection of a high-affinity peptide. (wikipedia.org)
  • Zasloff M (2002) Antimicrobial peptides of multicellular organisms. (oalib.com)
  • Gallo RL, Murakami M, Ohtake T, Zaiou M (2002) Biology and clinical relevance of naturally occurring antimicrobial peptides. (oalib.com)
  • The molecular basis for these phenomena has not been elucidated, despite extensive characterization of peptide requirements in alloreactivity and the solution of several molecular structures of the 2C and AHIII12.2 TCR allo- and xenomodels ( 2 )( 3 )( 4 )( 5 ). (rupress.org)
  • Isolation and structure-functional characterization of phage display library-derived mimotopes of noxiustoxin, a neurotoxin of the scorpion Centruroides noxius Hoffmann Molecular Immunology, 37, 755-766. (unam.mx)
  • Combinatorial chemistry in insects: a library of defensive macrocyclic polyamines. (nature.com)
  • Utilising its expertise in peptide chemistry and its novel technology platform, Xenome translates the evolutionary advantages inherent in venom peptides into new drug candidates. (biospace.com)
  • In this study, we evaluate Charged Surface Hybrid (CSH) C 18 column chemistry for peptide separations, expecting similar improvements in chromatographic performance since most peptides under commonly employed acidic conditions contain a positive charge. (waters.com)
  • This invention is directed to combinatorial chemistry libraries containing 3-aryl-2-indolylcarboxylic acids as well as solid phase methods for constructing such combinatorial chemistry libraries. (google.es)
  • Professor Hall joined the Stonehill Department of Chemistry in the Fall of 2000. (stonehill.edu)
  • Combinatorial peptide libraries displayed on phage have allowed rapid screening of billions of different peptides against a wide variety of target molecules (reviewed in 1-3). (neb.com)
  • A direct approach to identification of peptide mimics of antigen is through the use of peptide display libraries. (nih.gov)
  • 8. Smith MM, Shi L, Navre M. (1995) Rapid identification of highly active and selective substrates for stromelysin and matrilysin using bacteriophage peptide display libraries. (springer.com)
  • 2007) Identification of an ADAMTS-4 cleavage motif using phage display leads to the development of fluorogenic peptide substrates and reveals matrilin-3 as a novel substrate. (springer.com)
  • 2003) Identification of peptide substrates for human MMP-11 (stromelysin-3) using phage display. (springer.com)
  • Identification of Technetium-99m Binding Peptides Using Combinatorial Cellulose-Bound Peptide Libraries," J. Am. Chem. (patentgenius.com)
  • Brogden KA (2005) Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? (oalib.com)
  • Here, we have developed a methodology for bacterial cell sorting using the semi-automated autoMACS® Pro Separator for the first time, and have produced a complete method for sorting of bacteria displaying 15-mer peptides on their cell surface using this device, including downstream bioinformatic analysis of candidates for binding to a target of interest. (omicsonline.org)
  • As compared with yeast and phage display, bacterial display is ideal because of the fast doubling time of bacteria, about 20 minutes for Escherichia coli ( E. coli ) [ 7 ] versus about 2 hours for Saccharomyces cerevisiae ( S. cerevisiae ) [ 8 , 9 ], and direct amplification of the bound bacterial cells containing plasmid DNA encoding the displayed peptide responsible for binding, without elution and reinfection. (omicsonline.org)
  • By engineering a structural component of the fimbriae, FimH, to display a random peptide library, we were able to isolate metal-chelating bacteria. (dtu.dk)
  • Hetian and co-workers (21) panned against recombinant KDR (sKDR) to screen for peptides that would bind to KDR and potentially block its interaction with VEGF. (neb.com)
  • To analyze the peptide repertoire of alloreactive T cells and the potential influence of self-MHC molecules, we stimulated naive splenic T cells of MHC class I mutant or H2 recombinant mice against RMA-S cells (H2 b ) loaded with defined K b - or D b -specific peptide libraries (K b L, D b L) under limiting dilution conditions. (rupress.org)
  • Production, purification, and cleavage of tandem repeats of recombinant peptides. (currentprotocols.com)
  • Some combinations of peptides proved more inhibitory than did individual peptides. (asm.org)
  • Antimicrobial peptides (AMPs) are now regarded as a potential solution to revive the traditional methods of antibiotic development, although, until now, many AMPs have failed in clinical trials. (oalib.com)
  • Giuliani A, Rinaldi AC (2011) Beyond natural antimicrobial peptides: multimeric peptides and other peptidomimetic approaches. (oalib.com)
  • Brogden NK, Brogden KA (2011) Will new generations of modified antimicrobial peptides improve their potential as pharmaceuticals? (oalib.com)
  • Thomas S, Karnik S, Barai RS, Jayaraman VK, Idicula-Thomas S (2010) CAMP: a useful resource for research on antimicrobial peptides. (oalib.com)
  • Chemical diversity of the biologically active RXPs results from a combination of iterative and flexible use of monomodular nonribosomal peptide synthetases including substrate promiscuity, enzyme cross-talk and enzyme stoichiometry as shown by in vivo and in vitro experiments. (nature.com)
  • Finally, many peptides are biologically active, making them interesting as a class to the pharmaceutical industry. (google.es)
  • The libraries were built on scaffolds representing the most frequently used genes in human antibodies, which were diversified to mirror the variability of natural antibodies. (freepatentsonline.com)
  • Using a library of malondialdehyde (MDA)-modified polypeptides covering the complete amino acid sequence of human apoB-100, we have recently identified a large number of epitopes recognized by antibodies present in human plasma. (ahajournals.org)
  • 2. Berger A, Schechter I. (1970) Mapping the active site of papain with the aid of peptide substrates and inhibitors. (springer.com)
  • Karlsson R, Kullman-Magnusson M, Hamalainen MD, Remaeus A, Andersson K, Borg P, Gyzander E, Deinum J (2000) Biosensor analysis of drug-target interactions: direct and competitive binding assays for investigation of interactions between thrombin and thrombin inhibitors . (springer.com)
  • Construction of such a peptide-fused phage library possessing non-natural core structures will be useful for future drug discovery. (mdpi.com)
  • A metagenomic (community genomic) library consisting of 5,760 bacterial artificial chromosome clones was prepared in Escherichia coli DH10B from DNA extracted from the large-bowel microbiota of BALB/c mice. (asm.org)
  • Bacterial artificial chromosome (BAC) vectors are especially suitable in the preparation of metagenomic clone libraries because they stably maintain large DNA inserts (greater than 100 kbp) in Escherichia coli ( 3 ). (asm.org)
  • European Journal of Immunology , 2000, p. 1162 - 1171. (monash.edu)
  • One of the peptides, AAEEIYAARRG, is the best synthetic peptide substrate reported to date for HER2/Neu. (cshl.edu)
  • The predominant selected peptide (Pep1) was determined to bind both free HA and HA expressed on cell surfaces, as well as to block HA binding to cell surfaces expressing the HA receptor, CD44. (neb.com)
  • Screening of a mouse 17-day fetus cDNA library with the Angiotensin II receptor AT2 as the bait in yeast two-hybrid assay led us to identify an AT2-interacting mouse fetus peptide that shared 98% amino acid identity with the corresponding region of the human NHE6. (embl.de)
  • Kinetics of T-cell receptor binding by bivalent HLA-DR. Peptide complexes that activate antigen-specific human T-cells. (semanticscholar.org)
  • Monovalent major histocompatibility complex-peptide complexes dissociate within seconds from the T-cell receptor (TCR), indicating that dimerization/multimerization may be important during early stages of T-cell activation. (semanticscholar.org)
  • There was a marked trend for the peptides to inhibit SNV and HTNV to a greater extent than they inhibited PHV, a finding that supports the contention that SNV and HTNV use β 3 integrins and PHV uses a different receptor, β1 integrin. (asm.org)
  • B-type natriuretic peptide and adiponectin releases in rat model of myocardial damage induced by isoproterenol administration. (thefreelibrary.com)
  • B-type natriuretic peptide (BNP) is hormone secreted from cardiomyocytes in response to ventricular wall stretch and has been used as marker of ventricular dysfunction. (thefreelibrary.com)
  • Peptides that regulate the Water-electrolyte balance in the body, also known as natriuretic peptide hormones. (jove.com)
  • Subsequently, oral mucosal vaccines targeting DCs were proposed, involving Lactobacillus delivering protective antigens fused with specific DC-targeting peptides to DCs ( 13 ). (frontiersin.org)
  • Matzinger and Bevan ( 6 ) suggested that alloreactive T cells recognize many different cellular antigens, e.g., peptides, together with the foreign MHC. (rupress.org)
  • Later, Kaye and Janeway ( 7 ) and Bevan ( 8 ) proposed that alloreactive T cells see all cell surface MHC molecules regardless of peptides as antigens, leading to a high determinant density per target cell. (rupress.org)
  • However, a T cell repertoire, which contains T cells recognizing peptides bound to nonself-MHC molecules with high avidity contradicts the well-established bias of T cells to react against antigens preferentially in the context of self-MHC ( 29 ). (rupress.org)
  • These peptides inhibited viral entry in vitro as free peptides outside of the context of a phage. (asm.org)
  • For this aim, we propose a novel concept of pharmacophore generation from a drug-like molecule ( i.e. , salicylic acid) conjugated with surrounding randomized peptides. (mdpi.com)
  • There are also cases where the peptide changes structural characteristics of the MHC molecule and leads to recognition by certain T cell clones in a peptide-dependent rather than peptide-specific manner ( 16 )( 17 )( 18 ). (rupress.org)
  • Elution can be done combining low-pH elution buffer with sonification, which, in addition to loosening the peptide-target interaction, also serves to detach the target molecule from the immobilization surface. (wikipedia.org)
  • Lockhart, D.J. and Winzeler, E.A. (2000) Genomics, gene expression and DNA arrays. (springer.com)
  • A selected peptide sequence was found to inhibit proliferation of primary human vein endothelial cells, to have antiangiogenic effects in chick embryos, and finally to reduce breast tumor growth and metastasis by 70% in mice. (neb.com)
  • Selection of an immunogenic peptide mimic of the capsular polysaccharide of Neisseria meningitidis serogroup A using a peptide display library. (nih.gov)
  • These peptides are known as "carbohydrate-mimetic peptides (CMPs)" because they mimic carbohydrate structures. (hindawi.com)
  • Professor Hall's research program employs peptide phage display libraries to identify short peptides that can mimic the coordination of Zn 2+ in zinc-containing metalloenzymes. (stonehill.edu)
  • Bioactive peptides, which can be used as cell-targeting or gene delivery agents, have been identified either by panning against purified receptors (19-24) or against intact cells or tissue samples, both in vitro and in vivo (25-33). (neb.com)
  • These molecules, which are either constitutive or inducible, are recognized as important components of the innate defense system (Boman, 2000). (thefreelibrary.com)
  • The molecular orientation depended on the dipolar interaction between the peptide molecules and between the peptide dipole and its image. (springer.com)
  • Thus, the peptide repertoire of alloreactive T cells must be influenced by self-MHC molecules during positive or negative thymic selection or peripheral survival, much like the repertoire of the self-restricted T cells. (rupress.org)
  • In consequence, allorestricted, peptide-specific T cells (that are of interest for clinical applications) are easier to obtain if T cells and target cells express related MHC molecules. (rupress.org)
  • On the other hand, recognition of MHC molecules in the absence of peptides has been clearly demonstrated ( 14 )( 15 ). (rupress.org)
  • The involvement of peptides in alloreactivity that are present on stimulator cells in low copy numbers ( 3 )( 13 ) raised the possibility of generating allorestricted CTLs against tumor-associated self-peptides (self as seen from the tumor's host) presented by MHC molecules (nonself for the T cells) for adoptive immunotherapy ( 20 )( 21 )( 22 ). (rupress.org)
  • Such peptides have been demonstrated to bind to the single baculovirus IAP repeat (BIR) of ML-IAP and the third BIR of XIAP with similar high affinities (approximately 0.5 microM). (rcsb.org)
  • In enzymology, a peptide deformylase (EC 3.5.1.88) is an enzyme that catalyzes the chemical reaction formyl-L-methionyl peptide + H2O ⇌ {\displaystyle \rightleftharpoons } formate + methionyl peptide Thus, the two substrates of this enzyme are formyl-L-methionyl peptide and H2O, whereas its two products are formate and methionyl peptide. (wikipedia.org)
  • When the crystallographic- and nuclear magnetic resonance-based molecular modeling of the three-dimensional structure of CGRP, CT, amylin, and adrenomedullin peptides and their receptors is available, it will lead to a greater understanding of the involvement of this family of peptides in pathophysiology. (begellhouse.com)
  • Self-assembled monolayer (SAM) of peptides is investigated in terms of the correlation of their molecular orientation and the dipolar interactions. (springer.com)
  • The molecular direction against the substrate affects the orientation probably due to the interaction between the peptide dipole and Au-S polarization. (springer.com)
  • Combinatorial Libraries: Studies in Molecular Recognition and the Quest for New Catalysts," Liebigs Ann. (patentgenius.com)
  • Chemical combinatorial libraries are diverse collections of molecular compounds. (google.es)
  • Xenome is a biotechnology company focused on the discovery and development of peptide-based therapeutics. (biospace.com)
  • With increased use of peptides as therapeutics, the application space for this discovery platform has expanded in recent years [ 4 - 6 ]. (omicsonline.org)
  • A new algorithm, sequence-specific retention calculator, was developed to predict retention time of tryptic peptides during RP HPLC fractionation on C18, 300-A pore size columns. (nih.gov)
  • In addition, 8 of the 70 peptides had sequence similarity to SNV glycoproteins. (asm.org)
  • CGRP (a 37-amino-acid neuropeptide) and its receptors are widely distributed in the body, and it is the most potent endogenous vasodilatory peptide discovered so far. (begellhouse.com)
  • CT (a 32-amino-acid peptide) is, however, a hormone primarily involved in protecting the skeleton during periods of "calcium stress" such as growth, pregnancy, and lactation. (begellhouse.com)
  • Amylin (a 37-amino-acid peptide) is generated from a gene located on chromosome 12 (thought to be an evolutionary duplication of chromosome 11) and shares 46% amino acid sequence homology with CGRP and 20% with human CT. (begellhouse.com)
  • Adrenomedullin, a recently discovered 52-amino-acid vasoactive peptide from adrenal tissue, shares 24% homology with CGRP and is also a member of this superfamily of peptides. (begellhouse.com)
  • Hancock RE, Sahl HG (2006) Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies. (oalib.com)
  • The used helical peptides containing a disulfide group on the terminal, which can form SAMs on a gold substrate, were synthesized. (springer.com)
  • Such aptamers can be isolated from combinatorial libraries by SELEX (systematic evolution of ligands by exponential enrichment) [ 3 ]. (mdpi.com)
  • A combinatorial library comprising 46 million variants of the CBD domain was constructed through randomization of 11 positions located at the domain surface and distributed over three separate beta -sheets of the domain. (diva-portal.org)
  • Lennon GG, Lehrach H (1991) Hybridization analyses of arrayed cDNA libraries . (springer.com)
  • An automated prediction of MHC class I-binding peptides based on positional scanning with peptide libraries. (nig.ac.jp)
  • Six different peptide motifs were selected with the use of the mAb. (nih.gov)
  • The T7 promoter region allows large-scale in vitro T7 transcription to transcribe the DNA library into an mRNA library, which provides templates for the in vitro translation reaction later. (wikipedia.org)